title: Correlation of ECR1 A3650G polymorphism with neonatal respiratory distress syndrome.
abstract: OBJECTIVE
  This study aims to determine the correlation of erythrocyte complement receptor 1 (ECR1) A3650G (His1208Arg, rs2274567) polymorphism with the pathogenesis and development of neonatal respiratory distress syndrome (NRDS).
  
  
  METHODS
  This case-control study was conducted at the Huaihe Hospital of Henan University. Sixty-six infants who met the diagnostic criteria of NRDS were placed into the case group. There were 60 healthy infants without respiratory symptoms in the control group. Genetic analyses were conducted using peripheral blood-extracted genomic DNA. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied to detect SNP.
  
  
  RESULTS
  No apparent differences in gender, weight, and gestational age were observed between NRDS and healthy infants (all p>0.05). However, the NRDS group had more premature infants than the control group (59.1% vs. 38.3%, p=0.019). In the NRDS group, the premature infants had shorter gestational ages than the full-term infants, while the duration of mechanical ventilation and oxygen inhalation for premature infants was longer than those of full-term infants (all p<0.05). Genotype frequencies were in Hardy-Weinberg equilibrium in both the NRDS and control groups (both p>0.05). The results showed that the A carrier (AG/GG) and the A allele of the ECR1 A3650G polymorphism were associated with risk for NRDS (both p<0.05). Furthermore, premature infants with NRDS had higher frequencies of the A carrier (AG/GG) of ECR1 A3650G polymorphism than full-term infants (p=0.002).
  
  
  CONCLUSIONS
  Our findings provide evidence that the A carrier (AG/GG) and the A allele of the ECR1 A3650G polymorphism may be correlated to the pathogenesis of NRDS and, hence, might be involved in the susceptibility to NRDS.
authors.affiliation: 1 Department of Pediatrics, Huaihe Hospital, Henan University , Kaifeng, Henan, People's Republic of China .
authors.forename: Ying
authors.initials: Y
authors.lastname: Dai
authors.forename: Qi-Wei
authors.initials: QW
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: He
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
mesh_terms: D000483:Alleles; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007223:Infant; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D017463:Receptors, Complement 3b / Q000235:genetics*; D012127:Respiratory Distress Syndrome, Newborn / Q000235:genetics*
chemical_list: D017463:Receptors, Complement 3b