title: Immittance electroanalysis in diagnostics.
abstract: Impedance derived electroanalytical assays are inherently spectroscopic (frequency resolved) and potentially exceedingly sensitive indicators of interfacial change (such as target binding at an appropriate receptor). We introduce here the use of a portfolio of mathematically derived immittance functions and related components, capable, from the same raw data sets, of enabling increased assay sensitivity and markedly shorter assay times in comparison to traditional impedance analyses. The methodology, applied herein to faradaic (redox probe amplified) and non-faradaic assays, requires no equivalent circuit analysis or prior assumption of response. Its focus is to optimize analytical potency and to enable the user to select and apply the most frequency-optimized reporter of interfacial change and to, thereafter, run rapid (optimized) analyses at single frequencies.
authors.affiliation: Department of Chemistry, University of Oxford , South Parks Road, Oxford OX1 3QZ, United Kingdom.
authors.forename: Amol V
authors.initials: AV
authors.lastname: Patil
authors.forename: Flávio C
authors.initials: FC
authors.lastname: Bedatty Fernandes
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Bueno
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Davis
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis*; D003955:Diagnostic Tests, Routine; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D008433:Mathematics; D010084:Oxidation-Reduction
chemical_list: D006046:Gold; D002097:C-Reactive Protein