title: Epigenetic reprogramming by somatic cell nuclear transfer: questions and potential solutions.
abstract: Somatic cell nuclear transfer (SCNT) is a technology by which a highly differentiated somatic nucleus is transferred into an enucleated oocyte to generate a reconstructed embryo that subsequently develops to an offspring. However, to date, the efficiency of cloned animal is still low. The major reason is incomplete nuclear reprogramming of donor cells after nuclear transfer, which results in abnormal epigenetic modifications, including DNA methylation, histone acetylation, gene imprinting, X-chromosome inactivation, and telomere length. Most improvements have been made in somatic epigenetic reprogramming with small molecules and manipulating expression of specific genes. It is expected that SCNT will soon have broad applications in both basic research and practical production. In this review, we summarize the recent progress in epigenetic reprogramming by somatic cell nuclear transfer; in particular, we focus on strategies for rescuing the epigenetic errors occurring during SCNT.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China; Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
authors.forename: Ji
authors.initials: J
authors.lastname: Huili
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, Nanning 530004, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Haosheng
authors.affiliation: Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Dengke
mesh_terms: D000818:Animals; D065150:Cellular Reprogramming; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D018392:Genomic Imprinting; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D053652:Nuclear Transfer Techniques; D016615:Telomere
chemical_list: D056572:Histone Deacetylase Inhibitors