title: Biotransformation on the triterpenoid saponin of Ardisia gigantifolia by Aspergillus avenaceus AS 3.4454.
abstract: Compound 1, a triterpenoid saponin from Ardisia gigantifolia Stapf. showing potential anti-tumor activity, was transformed into three derivatives (2-4) by Aspergillus avenaceus 3.4454. Among them, compounds 2 and 3 are new compounds. Their structures were elucidated on the basis of 1D NMR, 2D NMR, HR-ESI-MS, and optical rotation data. Compounds 1-3 were evaluated for their cytotoxicity against human hepatocellular carcinoma and normal liver cells by cell counting kit 8 colorimetric assay. Compound 3 displayed better cytotoxicity against Bel-7402 and HepG2 cell lines and much weaker cytotoxicity against normal liver L02 cell than that of positive control (epirubicin hydrochloride).
authors.affiliation: a Department of Clinical Pharmacology , General Hospital of PLA , Beijing 100853 , China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mu
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Gu
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wang
authors.forename: Bai-Ping
authors.initials: BP
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031644:Ardisia / Q000737:chemistry*; D001230:Aspergillus / Q000378:metabolism; D001711:Biotransformation; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D012503:Saponins; D014315:Triterpenes; C087916:cyclamiretin A; D009828:Oleanolic Acid
keywords: Ardisia gigantifolia; Aspergillus avenaceus; biotransformation; triterpenoid saponin