title: Toxicological effects of short-term dietary acrylamide exposure in male F344 rats.
abstract: We recently reported that acrylamide, a known rodent and probable human carcinogen, does not increase the risk of azoxymethane (AOM)-induced rat colon precancerous lesions when administered through the diet. Here, we present toxicological data from non-AOM-injected rats. Briefly, male F344 rats were randomized into four dietary groups and received experimental diets based on AIN-93G formulation and containing acrylamide at 0 (control), 5, 10 or 50mg/kg diet (wt/wt) ad libitum for 10 weeks, after which they were killed and their blood collected for hematological and biochemical markers. Acrylamide at the higher doses (10 and 50mg/kg diet) significantly lowered (p<0.05) serum total high density lipoprotein and total testosterone and increased serum lipase in comparison to the control. Blood hematocrit values and lymphocyte counts were significantly lower (p<0.05) in the high dose acrylamide (50mg/kg diet) group compared to control, with a concomitant decrease in hemoglobin level, mean corpuscular volume and mean corpuscular hemoglobin. These results provide additional hazard characterization data and strengthen the notion that at high doses, acrylamide may involve systemic toxicity potentiating tumorigenesis in experimental animals. Further studies are required to understand the health effects of food-borne acrylamide, especially at the lower exposures typified by human diets.
authors.affiliation: Regulatory Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada. Electronic address: jayadev.raju@hc-sc.gc.ca.
authors.forename: Jayadev
authors.initials: J
authors.lastname: Raju
authors.affiliation: Regulatory Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Roberts
authors.affiliation: Regulatory Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Marnie
authors.initials: M
authors.lastname: Taylor
authors.affiliation: Scientific Services Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Dominique
authors.initials: D
authors.lastname: Patry
authors.affiliation: Scientific Services Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Emily
authors.initials: E
authors.lastname: Chomyshyn
authors.affiliation: Scientific Services Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Don
authors.initials: D
authors.lastname: Caldwell
authors.affiliation: Regulatory Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Gerard
authors.initials: G
authors.lastname: Cooke
authors.affiliation: Regulatory Toxicology Research Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch Health Canada, Ottawa, Ontario, Canada.
authors.forename: Rekha
authors.initials: R
authors.lastname: Mehta
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D004032:Diet; D006400:Hematocrit; D008049:Lipase / Q000097:blood; D008075:Lipoproteins, HDL / Q000097:blood; D018655:Lymphocyte Count; D008297:Male; D011916:Rats, Inbred F344; D013739:Testosterone / Q000097:blood
chemical_list: D002273:Carcinogens; D008075:Lipoproteins, HDL; D020106:Acrylamide; D013739:Testosterone; D008049:Lipase
keywords: Acrylamide; Clinical biochemistry; Diet; F344 rats; Hematology; Testosterone