title: [Changes of total flavonoids and rutin contents in different parts of wild and cultivated Hypericum attenuatum ].
abstract: OBJECTIVE
  To determine the content of total flavonoids and rutin in different parts of wild and cultivated Hypericum attenuatum.
  
  
  METHODS
  Ethanol ultrasonic extraction method was used to extract total flavonoids and rutin in different parts of Hypericum attenuatum. With rutin as reference substance, UV spectrophotometry and high performance liquid chromatography were applied to deter- mine the total flavonoids and rutin content in Hypericum attenuatum samples (leaves, stems and flowers).
  
  
  RESULTS
  There were great differences between the total flavonoids in different parts of Hypericum attenuatum that the content of flavonoids in the wild one was leaves > flowers > stems; The content of flavonoids in the cultivated was leaves > flowers > fruits > stems. The content of flavonoids in each part of cultivated Hypericum attenuatum was higher than that in the wild,but did not reach a statistically significant level,while the difference of content of rutin was extremely significant.
  
  
  CONCLUSIONS
  There are differences between the content of flavonoids in different parts of Hypericum attenuatum. The highest content is found in leaves and the lowest in stems. The content of flavonoids in cultivated Hypericum attenuatum is higher than that in the wild.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.forename: Nan-yi
authors.initials: NY
authors.lastname: Zhang
authors.forename: Gui-ying
authors.initials: GY
authors.lastname: Chang
authors.forename: Feng-yan
authors.initials: FY
authors.lastname: Xiao
authors.forename: Ke-qin
authors.initials: KQ
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D035264:Flowers; D005638:Fruit; D020902:Hypericum / Q000737:chemistry*; D018515:Plant Leaves; D012431:Rutin / Q000032:analysis*
chemical_list: D005419:Flavonoids; D012431:Rutin