title: Rat Articular Cartilages Change Their Tissue and Protein Compositions During Perinatal Period.
abstract: Articular cartilage (AC) covers the surface of bones in joints and functions as a cushion against mechanical loading. The tissue contains abundant extracellular matrix (ECM), which mainly consists of proteoglycans (PG) and collagen (COL) fibres. The property of AC is gradually changing by ageing with gravity loading. To know the property change of AC by initial gravity loading during short period after birth, we performed histological assays and proteomics assay on the AC of the femoral condyle in knee joints of perinatal rats. The water content (%) was significantly decreased in neonate AC compared with fetal AC. During the perinatal stages (E19 and P0), the localizations of glycosaminoglycan (GAG) and type I and II COLs were homogeneous. The density of chondrocytes was significantly decreased in the deeper layers comparing with the surface layer in neonate AC. In addition, we found a drastic change in the protein expression pattern on proteomic analysis. The expressions of ECM components were relatively increased in neonate AC compared with fetal AC.
authors.affiliation: Department of Public Health, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi-Miura
authors.affiliation: Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyusyu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Osago
authors.affiliation: Department of Anatomy and Cell Biology, Graduate School of Medicine, Kyusyu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamaguchi
authors.affiliation: Department of Human Health Sciences, Graduate School of Medicine, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aoyama
authors.affiliation: Department of Public Health, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minamikogushi, Ube, Yamaguchi, 755-8505, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tanabe
authors.affiliation: Department of Biosignaling and Radioisotope Experiment, Interdisciplinary Center for Science Research, Organization for Research, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: K-I
authors.initials: KI
authors.lastname: Matsumoto
authors.affiliation: Department of Public Health, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane, 693-8501, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology* / Q000254:growth & development; D001834:Body Water; D002358:Cartilage, Articular / Q000737:chemistry* / Q000166:cytology / Q000196:embryology / Q000254:growth & development*; D002452:Cell Count / Q000662:veterinary; D019902:Chondrocytes / Q000166:cytology; D005260:Female; D007719:Knee Joint / Q000033:anatomy & histology; D011247:Pregnancy; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar / Q000033:anatomy & histology* / Q000196:embryology / Q000235:genetics / Q000254:growth & development
chemical_list: D011506:Proteins