title: A near infrared spectroscopic assay for stalk soluble sugars, bagasse enzymatic saccharification and wall polymers in sweet sorghum.
abstract: In this study, 123 sweet sorghum (Sorghum bicolor L.) accessions and 50 mutants were examined with diverse stalk soluble sugars, bagasse enzymatic saccharification and wall polymers, indicating the potential near infrared spectroscopy (NIRS) assay for those three important parameters. Using the calibration and validation sets and modified squares method, nine calibration optimal equations were generated with high determination coefficient on the calibration (R(2)) (0.81-0.99), cross-validation (R(2)cv) (0.77-0.98), and the ratio performance deviation (RPD) (2.07-7.45), which were at first time applied by single spectra for simultaneous assay of stalk soluble sugars, bagasse hydrolyzed sugars, and three major wall polymers in bioenergy sweet sorghum.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Leiming
authors.initials: L
authors.lastname: Wu
authors.affiliation: Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; National Energy R&D Center for Non-food Biomass, Beijing 100193, China; College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Huang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Zou
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Hu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Chunfen
authors.initials: C
authors.lastname: Fan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Haichun
authors.initials: H
authors.lastname: Jing
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Peng
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: fengsq@mail.hzau.edu.cn.
authors.forename: Shengqiu
authors.initials: S
authors.lastname: Feng
mesh_terms: D018533:Biomass; D001704:Biopolymers / Q000737:chemistry*; D002138:Calibration; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis*; D002473:Cell Wall / Q000737:chemistry*; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D006868:Hydrolysis; D015203:Reproducibility of Results; D012995:Solubility; D045868:Sorghum / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D001704:Biopolymers; D002241:Carbohydrates; D002482:Cellulose; C027433:bagasse; D002480:Cellulase
keywords: Bioenergy crops; Cell wall polymers; Fermentable sugars; Near infrared spectroscopy (NIRS); Sweet sorghum