title: [Protective effect of gangliosides against the toxic action of bacterial lipopolysaccharide of various serotypes on PC12 cells].
abstract: The effect of bacterial lipopolysaccharide (LPS) from E. coli on the viability of PC12 neuronal cell line was studied. LPS of 0111:B4 serotype and LPS of 055:B5 serotype were shown to have toxic effect on PC12 cells. But the toxic effect of LPS of 0127:B8 serotype was not revealed. Preincubation of PC12 cells with GM1 or GD1a gangliosides was found to diminish significantly the death of the cells and the formation of reactive oxygen species induced by LPS of 0111:B4 and 055:B5 serotypes in them. The protective effect of GM1 and GD1a was found not to depend on the presence of the inhibitor of Trk receptor tyrosine kinase in the medium, though activation of this protein kinase was previously shown to mediate the protective effect of gangliosides against the action of excitatory amino acids, pro-oxidants and other toxins on neurons and cells of neuronal cell lines. The protective effect of gangliosides against the LPS action on PC12 cells was similar to the effect of methyl-beta-cyclodextrin, which was shown to disturb cell membrane raft structure. The suggestion is put forward that the pronounced diminution of toxic effect of LPS on PC12 cells by gangliosides may be explained by the alteration of structural organization of lipid rafts caused by the incorporation of exogenous gangliosides in cell plasma membranes, which diminishes the translocation of TLR4 receptor into the rafts and their activation by LPS.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Vlasova
authors.forename: L V
authors.initials: LV
authors.lastname: Baiunova
authors.forename: E I
authors.initials: EI
authors.lastname: Mikurova
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Liakhovnenko
authors.forename: S D
authors.initials: SD
authors.lastname: Nikolaeva
authors.forename: R G
authors.initials: RG
authors.lastname: Parnova
authors.forename: N F
authors.initials: NF
authors.lastname: Avrova
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D004926:Escherichia coli / Q000737:chemistry; D005732:Gangliosides / Q000008:administration & dosage*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D065288:Serogroup; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: D005732:Gangliosides; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D051197:Toll-Like Receptor 4; C021298:ganglioside, GD1a; D006861:Hydrogen Peroxide