title: Living cells imaging for copper and hydrogen sulfide by a selective "on-off-on" fluorescent probe.
abstract: A novel highly selective and sensitive fluorescent probe (NJ1) had been designed and synthesized for Cu(2+) detection by fluorescence quenching mechanism, and then the enhancement of fluorescence intensity with the addition of hydrogen sulfide in complex NJ1Cu aqueous solution at physiological conditions were described. This "on-off-on" type fluorescence recognition system was a reversible process, which could be utilized to monitor copper ion and hydrogen sulfide based on a complex NJ1Cu formation-Cu(2+) displacement approach. Importantly, this real-time recognition process of Cu(2+) and hydrogen sulfide exhibited excellent anti-interference ability. In addition, this new fluorescent probe also has potential utility for Cu(2+) and hydrogen sulfide detection in living cells, providing a potential tool for investigating copper ion and hydrogen sulfide in living systems or environment.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. Electronic address: yongqian@nju.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China.
authors.forename: Tianbao
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China. Electronic address: zhuhl@nju.edu.cn.
authors.forename: Hailiang
authors.initials: H
authors.lastname: Zhu
mesh_terms: D003300:Copper / Q000032:analysis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000138:chemical synthesis / Q000737:chemistry*; D006862:Hydrogen Sulfide / Q000032:analysis*; D057054:Molecular Imaging / Q000379:methods*; D009284:Naphthols / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: C000600056:7-(dimethylamino)-3-((2-(pyridine-2-yl)hydrazono)methyl)naphthalene-2-ol; D005456:Fluorescent Dyes; D006835:Hydrazones; D009284:Naphthols; D003300:Copper; D006862:Hydrogen Sulfide
keywords: Copper ion; Displacement approach; Fluorescent probe; Hydrogen sulfide; Imaging