title: [Effect of glucose regulated protein 78 gene silencing on hyperoxia-induced apoptosis in alveolar epithelial cells].
abstract: OBJECTIVE
  To investigate the effect and possible mechanism of small interfering RNA (siRNA)-mediated glucose regulated protein 78 (GRP78) gene silencing on hyperoxia-induced apoptosis of alveolar epithelial cells.
  
  
  METHODS
  Human lung adenocarcinoma A549 cells in vitro were transfected with siRNA by Lipofectamine(TM)2000. Cells were divided into untransfected group, scrambled siRNA group and GRP78-siRNA group. A model of hyperoxia-induced cell injury was established by 95% oxygen. After 48 hours, the mRNA and protein levels of GRP78 and C/EBP homologous protein (CHOP) were detected respectively by real-time PCR and Western blotting, and cell apoptosis was measured by flow cytometry.
  
  
  RESULTS
  By GRP78 siRNA interference, the mRNA and protein levels of GRP78 decreased significantly, while CHOP increased significantly. The apoptosis rate of A549 cells was elevated significantly.
  
  
  CONCLUSIONS
  GRP78 gene silencing may enhance the CHOP apoptotic pathway and promote apoptosis in alveolar epithelial cells induced by hyperoxia, suggesting the potential value of GRP78 as a therapeutic target for the clinical treatment of bronchopulmonary dysplasia.
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Chuchu
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Pediatrics, Affiliated Hospital of Jiangsu University, Zhenjiang 212000, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis; D001997:Bronchopulmonary Dysplasia / Q000209:etiology* / Q000628:therapy; D015687:Cell Hypoxia; D000091342:Endoplasmic Reticulum Chaperone BiP; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D020868:Gene Silencing; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D011650:Pulmonary Alveoli / Q000166:cytology; D034741:RNA, Small Interfering / Q000235:genetics; D051742:Transcription Factor CHOP / Q000235:genetics / Q000502:physiology
chemical_list: C497249:DDIT3 protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D034741:RNA, Small Interfering; D051742:Transcription Factor CHOP