title: Gaussian beam in two-photon fluorescence imaging of rat brain microvessel.
abstract: The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.
authors.affiliation: The City College of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031, United StatesbThe City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City Colle.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Shi
authors.affiliation: The City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Conve.
authors.forename: Adrián
authors.initials: A
authors.lastname: Rodríguez-Contreras
authors.affiliation: The City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Convent Avenue, New York, New York 10031, United States.
authors.forename: Robert R
authors.initials: RR
authors.lastname: Alfano
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply*; D002560:Cerebrovascular Circulation; D005260:Female; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D055806:Microvessels / Q000033:anatomy & histology*; D051381:Rats; D017207:Rats, Sprague-Dawley