title: Changes of spontaneous parthenogenetic activation and development potential of golden hamster oocytes during the aging process.
abstract: The golden hamster is an excellent animal experimental model for oocyte research. The hamster oocytes are very useful in clinical examination of human spermatozoan activity. Non-fertile oocytes can lead to time-dependent processes of aging, which will affect the results of human spermatozoa examination. As a consequence there is a need to investigate the aging and anti-aging processes of golden hamster oocytes. In order to study the aging processes and parthenogenetic activation of golden hamster oocytes, in vivo oocytes, oocytes cultured with or without cumulus cells, and oocytes treated with Trichostatin A (TSA) or caffeine were collected and investigated. We found that: (1) spontaneous parthenogenetic activation, developmental potential (cleavage rate), and zona pellucida (ZP) hardening undergo age-dependent changes in in vivo, in vitro, and after TSA or caffeine treatment; (2) in vivo, oocytes became spontaneously parthenogenetic 25 h post-hCG treatment; (3) in vitro, cumulus cells did not significantly increase the parthenogenetic activation rate of cultured hamster oocytes; and (4) TSA or caffeine could delay spontaneous oocyte parthenogenetic activation and the aging processes by at least 5h, but also accelerated the hardening of the ZP. These results define the conditions for the aging and anti-aging processes in golden hamster oocytes. TSA and caffeine play roles in controlling spontaneous activation, which could facilitate the storage and use of golden hamster oocytes for studying processes relevant to human reproduction.
authors.affiliation: Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
authors.forename: Han
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Ce
authors.initials: C
authors.lastname: Wang
authors.affiliation: Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Guan
authors.affiliation: Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China. Electronic address: wangli@ohsu.edu.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China. Electronic address: ziyi_li86@163.com.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Li
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008647:Mesocricetus; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010312:Parthenogenesis; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D010726:Phosphodiesterase Inhibitors; D002110:Caffeine; C012589:trichostatin A
keywords: Caffeine; Cortical granules; Cumulus cells; Trichostatin A; Zona pellucida