title: Synergistic anticancer activity of fluorescent copper nanoclusters and cisplatin delivered through a hydrogel nanocarrier.
abstract: Highly fluorescent red copper nanoclusters (Cu NCs) were synthesized in aqueous medium in the presence of dihydrolipoic acid and poly(vinylpyrrolidone) (PVP). The Cu NCs, in solid form, were stable, retained their optical properties for a month, and could be redispersed for use when required. The NCs in aqueous medium exhibited pH-tunable reversible optical properties. The PVP stabilized NCs, when converted into hydrogel by cross-linking with poly(vinyl alcohol), delivered anticancer drug to cervical cancer (HeLa) cells, thereby inducing apoptotic cell death. The red emission properties of the Cu NCs in the hydrogel were used for optical imaging as well as for flow cytometric probe of cellular uptake. Cell viability assay, Caspase3 assay, and cell cycle analyses demonstrated that the Cu NCs present in the hydrogel composite exhibited synergy of action, along with the drug, cisplatin, against HeLa cells.
authors.affiliation: Department of Chemistry, ‡Centre for Nanotechnology, and §Department of Biotechnology, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
authors.forename: Rama
authors.initials: R
authors.lastname: Ghosh
authors.forename: Upashi
authors.initials: U
authors.lastname: Goswami
authors.forename: Siddhartha Sankar
authors.initials: SS
authors.lastname: Ghosh
authors.forename: Anumita
authors.initials: A
authors.lastname: Paul
authors.forename: Arun
authors.initials: A
authors.lastname: Chattopadhyay
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D002945:Cisplatin / Q000737:chemistry / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000295:instrumentation*; D004357:Drug Synergism; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D003300:Copper; D002945:Cisplatin
keywords: biolabeling; cisplatin; copper nanoclusters; drug delivery; hydrogel