title: MMP-9 expression is increased in B lymphocytes during multiple sclerosis exacerbation and is regulated by microRNA-320a.
abstract: B cells are necessary to maintain disease activity in relapsing multiple sclerosis (MS) and produce matrix metallopeptidase-9 (MMP-9), which disrupts the blood-brain barrier. MMP-9 protein expression was increased and expression of microRNA-320a (miR-320a), which targets MMP-9 mRNA, was significantly decreased in B lymphocytes of MS patients during a disease relapse compared to remission. Functional significance of these findings was demonstrated by transfecting human B lymphocytes with miR-320a inhibitor, which led to increased MMP-9 expression and secretion. In summary, expression of miR-320a is decreased in B cells of MS patients and may contribute to increased blood-brain barrier permeability and neurological disability.
authors.affiliation: Department of Neurology, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street, New Brunswick, NJ 08901, United States.
authors.forename: Latt Latt
authors.initials: LL
authors.lastname: Aung
authors.affiliation: Department of Neurology, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street, New Brunswick, NJ 08901, United States.
authors.forename: M Maral
authors.initials: MM
authors.lastname: Mouradian
authors.affiliation: Department of Neurology, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street, New Brunswick, NJ 08901, United States.
authors.forename: Suhayl
authors.initials: S
authors.lastname: Dhib-Jalbut
authors.affiliation: Department of Neurology, Rutgers-Robert Wood Johnson Medical School, 125 Paterson Street, New Brunswick, NJ 08901, United States. Electronic address: konstantin.balashov@rutgers.edu.
authors.forename: Konstantin E
authors.initials: KE
authors.lastname: Balashov
mesh_terms: D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000201:enzymology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D055815:Young Adult
chemical_list: D015850:Interleukin-6; C572916:MIRN320 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9
keywords: B lymphocytes; MMP-9; MicroRNA; Multiple sclerosis; miR-320a