title: New type of Sendai virus vector provides transgene-free iPS cells derived from chimpanzee blood.
abstract: Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation and the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into the cellular DNA, and can be easily eliminated. TS12KOS carries KLF4, OCT3/4, and SOX2 in a single vector and can easily generate iPSCs from human blood cells. Using TS12KOS, we established iPSC lines from chimpanzee blood, and used DNA array analysis to show that the global gene-expression pattern of chimpanzee iPSCs is similar to those of human embryonic stem cell and iPSC lines. These results demonstrated that our new vector is useful for generating iPSCs from the blood cells of both human and chimpanzee. In addition, the chimpanzee iPSCs are expected to facilitate unique studies into human physiology and disease.
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Fujie
authors.affiliation: DNAVEC Corporation, 6 Ookubo, Tsukuba, Ibaragi 300-2611, Japan; Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, Japan.
authors.forename: Noemi
authors.initials: N
authors.lastname: Fusaki
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Katayama
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hamasaki
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Soejima
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Minami
authors.initials: M
authors.lastname: Soga
authors.affiliation: DNAVEC Corporation, 6 Ookubo, Tsukuba, Ibaragi 300-2611, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ban
authors.affiliation: DNAVEC Corporation, 6 Ookubo, Tsukuba, Ibaragi 300-2611, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: Department of Neurology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamashita
authors.affiliation: Department of Child Development, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Section of Comparative Microbiology and Immunology, Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Section of Language and Intelligence, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Matsuzawa
authors.affiliation: Section of Comparative Microbiology and Immunology, Center for Human Evolution Modeling Research, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan; Laboratory of Evolutional Virology, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, Kyoto, 606-8507, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Akari
authors.affiliation: Department of Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Era
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D065150:Cellular Reprogramming / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D000090062:Kruppel-Like Factor 4; D002679:Pan troglodytes; D029082:Sendai virus / Q000235:genetics*; D014161:Transduction, Genetic; D019076:Transgenes