title: MiR-21 and miR-222 inhibit apoptosis of adult dorsal root ganglion neurons by repressing TIMP3 following sciatic nerve injury.
abstract: MicroRNAs (miRNAs or miRs) are involved in phenotype modulation of neural cells after peripheral nerve injury. The effects of miRNAs on the survival of dorsal root ganglion (DRG) neurons, however, have not yet been well understood. In this study, microarray profiling indicated that 13 miRNAs were differentially expressed in rat DRGs (L4-L6) during the initial 7d period post sciatic nerve transection, and that the expressions of miR-21 and miR-222 (2 out of the 13 miRNAs) were continually increased over the time period. Tissue inhibitor of metalloproteinase 3 (TIMP3), a pro-apoptotic protein in various cancer cells, was identified as a common target of miR-21 and miR-222. Over-expression of miR-21 and miR-222 inhibited cell apoptosis and enhanced cell viability in cultured DRG neurons. IL-6 could induce up-regulation of miR-21 expression. All the results showed that miR-21 and miR-222 inhibited neuronal apoptosis at least partially through suppressing TIMP3 after peripheral nerve injury.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou, JS 215123, PR China; Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Shibo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Yi
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Key Lab of Systems Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China. Electronic address: nervegu@ntu.edu.cn.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Gu
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou, JS 215123, PR China; Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, 19 Qixiu Road, Nantong, JS 226001, PR China. Electronic address: dingfei@ntu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ding
mesh_terms: D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000473:pathology*; D057809:HEK293 Cells; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000473:pathology*; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000473:pathology*; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics / Q000378:metabolism
chemical_list: D015850:Interleukin-6; C568897:MIRN222 microRNA, rat; D035683:MicroRNAs; D019717:Tissue Inhibitor of Metalloproteinase-3; C546082:mirn21 microRNA, rat
keywords: Apoptosis; Dorsal root ganglion neuron; TIMP3; miR-21; miR-222