title: A new direct laser photo-induced fluorescence method coupled on-line with liquid chromatographic separation for the simultaneous determination of anilides pesticides.
abstract: A new direct laser photo-induced fluorescence high performance liquid chromatography (DL-PIF-HPLC) method is developed for the simultaneous determination of three anilide pesticides, namely carboxin, monalide and propanil. DL-PIF-HPLC uses a tunable Nd:YAG-OPO laser to obtain fluorescent photoproduct(s) and to simultaneously analyze their fluorescence in a short acquisition time with an intensified CCD camera, which improves the selectivity (by choosing the suitable excitation wavelength), increases the sensitivity (due to the high energy of the laser beam) and reduces the time of analysis, relative to the classical PIF methods. However, one of the main drawbacks of PIF methods is the presence of interferences with other compounds, such as other pesticides from the same group yielding similar fluorescent photoproducts, which reduces their selectivity. The analytical interest of DL-PIF-HPLC to avoid these interferences is demonstrated. The DL-PIF spectra, chromatographic conditions and analytical performances of DL-PIF-HPLC are presented for the simultaneous determination of three anilide pesticides. The calibration curves are linear over one order of magnitude and the limits of detection are in the ng mL(-1) range. The new DL-PIF-HPLC system has the advantage to combine the performances of both techniques, DL-PIF and liquid chromatography, and to improve the analysis selectivity.
authors.affiliation: Laboratoire de Photochimie et d׳Analyse, Faculté des Sciences et Techniques, Université C.A. Diop, Dakar, Sénégal.
authors.forename: O M A
authors.initials: OM
authors.lastname: Mbaye
authors.affiliation: Université de Brest, UMR CNRS 6521, Faculté des Sciences, Université de Brest, 6 Avenue Victor Le Gorgeu, 29285 Brest Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Maroto
authors.affiliation: Laboratoire de Photochimie et d׳Analyse, Faculté des Sciences et Techniques, Université C.A. Diop, Dakar, Sénégal.
authors.forename: M D
authors.initials: MD
authors.lastname: Gaye-Seye
authors.affiliation: Université de Brest, UMR CNRS 6521, Faculté des Sciences, Université de Brest, 6 Avenue Victor Le Gorgeu, 29285 Brest Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Stephan
authors.affiliation: Université de Brest, UMR CNRS 6521, Faculté des Sciences, Université de Brest, 6 Avenue Victor Le Gorgeu, 29285 Brest Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Deschamps
authors.affiliation: Université Paris-Est, Laboratoire Géomatériaux et Environnement (EA 4508), UPEMLV, 77454 Marne-la-Vallée Cedex 2, France.
authors.forename: J J
authors.initials: JJ
authors.lastname: Aaron
authors.affiliation: Université de Brest, UMR CNRS 6521, Faculté des Sciences, Université de Brest, 6 Avenue Victor Le Gorgeu, 29285 Brest Cedex, France. Electronic address: philippe.giamarchi@univ-brest.fr.
authors.forename: P
authors.initials: P
authors.lastname: Giamarchi
mesh_terms: D002138:Calibration; D002261:Carboxin / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008027:Light; D057230:Limit of Detection; D000432:Methanol; D010575:Pesticides / Q000032:analysis*; D011411:Propanil / Q000032:analysis*; D012996:Solutions; D012997:Solvents; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014867:Water
chemical_list: D010575:Pesticides; D012996:Solutions; D012997:Solvents; D014867:Water; D002261:Carboxin; D011411:Propanil; D000432:Methanol
keywords: Anilide pesticides; HPLC; Laser fluorescence; Photo-induced fluorescence (PIF); Photoproducts