title: Comparative analysis of genes regulated by Dzip1/iguana and hedgehog in zebrafish.
abstract: BACKGROUND
  The zebrafish genetic mutant iguana (igu) has defects in the ciliary basal body protein Dzip1, causing improper cilia formation. Dzip1 also interacts with the downstream transcriptional activators of Hedgehog (Hh), the Gli proteins, and Hh signaling is disrupted in igu mutants. Hh governs a wide range of developmental processes, including stabilizing developing blood vessels to prevent hemorrhage. Using igu mutant embryos and embryos treated with the Hh pathway antagonist cyclopamine, we conducted a microarray to determine genes involved in Hh signaling mediating vascular stability.
  
  
  RESULTS
  We identified 40 genes with significantly altered expression in both igu mutants and cyclopamine-treated embryos. For a subset of these, we used in situ hybridization to determine localization during embryonic development and confirm the expression changes seen on the array.
  
  
  CONCLUSIONS
  Through comparing gene expression changes in a genetic model of vascular instability with a chemical inhibition of Hh signaling, we identified a set of 40 differentially expressed genes with potential roles in vascular stabilization.
authors.affiliation: Department of Biochemistry and Molecular Biology and Alberta Children's Hospital Research Institute, University of Calgary, Canada.
authors.forename: Corey R
authors.initials: CR
authors.lastname: Arnold
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Lamont
authors.forename: John T
authors.initials: JT
authors.lastname: Walker
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Spice
authors.forename: Chi-Kin
authors.initials: CK
authors.lastname: Chan
authors.forename: Chi-Yip
authors.initials: CY
authors.lastname: Ho
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Childs
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D015533:Transcriptional Activation / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics
chemical_list: D002352:Carrier Proteins; C488008:Dzip1 protein, zebrafish; D053823:Hedgehog Proteins
keywords: cyclopamine; hemorrhage; microarray