title: Development and validation of a screening procedure of microalgae for biodiesel production: application to the genus of marine microalgae Nannochloropsis.
abstract: Nannochloropsis has emerged as a promising alga for biodiesel production. However, the genus consists of 6 species and hundreds of strains making strain selection a challenge. Furthermore, oil productivity is instrumental to economic viability of any algal strain for industrial production, which is dependent on growth rate and oil content. In most cases, these two parameters have been studied independently. Thus, the goal of this study is to provide a combined method for evaluating strain performance in specially designed photobioreactors together with an in-depth lipidomic analyses. The nine strains of Nannochloropsis tested showed considerable variations in productivity and lipidomics highlighting the importance of strain selection. Finally, Nannochloropsis gaditana CCMP527 and Nannochloropsis salina CCMP537 emerged as the two most promising strains, with an oil content of 37 and 27 dry wt% after 11-day nitrogen starvation, respectively, resulting in TAG productivity of 13×10(-3) and 18×10(-3) kg m(-3) d(-1), respectively.
authors.affiliation: GEPEA, Université de Nantes, CNRS, UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France; Department of Food Science and Technology, Faculty of Agricultural and Veterinary Medicine, Lebanese University, Dekwaneh, Beirut, Lebanon; AZM Center for Biotechnology Research and its Applications, Laboratory of Applied Biotechnology, Lebanese University, EDST, Tripoli, Lebanon.
authors.forename: A
authors.initials: A
authors.lastname: Taleb
authors.affiliation: GEPEA, Université de Nantes, CNRS, UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France. Electronic address: jeremy.pruvost@univ-nantes.fr.
authors.forename: J
authors.initials: J
authors.lastname: Pruvost
authors.affiliation: GEPEA, Université de Nantes, CNRS, UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Legrand
authors.affiliation: GEPEA, Université de Nantes, CNRS, UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: H
authors.initials: H
authors.lastname: Marec
authors.affiliation: GEPEA, Université de Nantes, CNRS, UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: B
authors.initials: B
authors.lastname: Le-Gouic
authors.affiliation: CEA, IBEB, Lab Bioenerget Biotechnol Bacteries & Microalgues, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix Marseille Université, BVME UMR7265, Marseille, F-13284, France.
authors.forename: B
authors.initials: B
authors.lastname: Mirabella
authors.affiliation: CEA, IBEB, Lab Bioenerget Biotechnol Bacteries & Microalgues, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix Marseille Université, BVME UMR7265, Marseille, F-13284, France.
authors.forename: B
authors.initials: B
authors.lastname: Legeret
authors.affiliation: CEA, IBEB, Lab Bioenerget Biotechnol Bacteries & Microalgues, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix Marseille Université, BVME UMR7265, Marseille, F-13284, France.
authors.forename: S
authors.initials: S
authors.lastname: Bouvet
authors.affiliation: CEA, IBEB, Lab Bioenerget Biotechnol Bacteries & Microalgues, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix Marseille Université, BVME UMR7265, Marseille, F-13284, France.
authors.forename: G
authors.initials: G
authors.lastname: Peltier
authors.affiliation: CEA, IBEB, Lab Bioenerget Biotechnol Bacteries & Microalgues, Saint-Paul-lez-Durance, F-13108, France; CNRS, UMR 7265 Biol Veget & Microbiol Environ, Saint-Paul-lez-Durance, F-13108, France; Aix Marseille Université, BVME UMR7265, Marseille, F-13284, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Li-Beisson
authors.affiliation: Department of Food Science and Technology, Faculty of Agricultural and Veterinary Medicine, Lebanese University, Dekwaneh, Beirut, Lebanon.
authors.forename: S
authors.initials: S
authors.lastname: Taha
authors.affiliation: AZM Center for Biotechnology Research and its Applications, Laboratory of Applied Biotechnology, Lebanese University, EDST, Tripoli, Lebanon.
authors.forename: H
authors.initials: H
authors.lastname: Takache
mesh_terms: D059001:Aquatic Organisms / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002853:Chromatography, Liquid; D005227:Fatty Acids / Q000096:biosynthesis; D007700:Kinetics; D013058:Mass Spectrometry; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*; D058433:Photobioreactors / Q000382:microbiology; D015203:Reproducibility of Results; D058009:Stramenopiles / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D014280:Triglycerides
keywords: Biodiesel; Lipids; Microalgae; Photobioreactors; Triglycerides