title: CirculatingSFRP1promoter methylation status in gastric adenocarcinoma and esophageal square cell carcinoma.
abstract: The secreted frizzled-related protein 1 (SFRP1) gene plays an important role in carcinogenesis of digestive system cancer. Previous studies proved that circulating DNA promoter methylation may be a suitable biomarker for cancer patients. The aim of the present study was to investigate whether the promoter methylation status of serumSFRP1is a potential biomarker for gastric adenocarcinoma (GAC) and esophageal square cell carcinoma (ESCC). The blood samples obtained from 42 GAC and 36 ESCC patients were detected for the promoter methylation status ofSFRP1by methylation-specific polymerase chain reaction. The control group included 42 benign gastrointestinal disease volunteers (24 benign gastric disease and 18 benign esophageal disease) and 20 healthy volunteers. SerumSFRP1methylation was evident in 30.95% (13/42) GAC patients and 38.89% (14/36) ESCC patients, which is clearly higher compared to 8.33% (2/24) in benign gastric disease, 11.11% (2/18) in benign esophageal disease and 5% (1/20) in healthy volunteers (P<0.05). The association between the serumSFRP1promoter methylation status and the clinical pathological features were further analyzed and methylation of theSFRP1gene was significantly associated with age >60 years in GAC patients (P=0.027). However, no correlations between theSFRP1methylation status and other clinicopathological parameters were found. In conclusion, theSFRP1promoter was detected frequently in the serum of GAC and ESCC patients. The detection of circulating methylatedSFRP1in the serum may be a useful biomarker for upper gastrointestinal cancer patients.
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Heng
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Zhengkai
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Youke
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Gastroenterology and Hepatology, Jinling Hospital, School of Medicine, Southern Medical University, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Fangyu
authors.initials: F
authors.lastname: Wang
keywords: SFRP1 gene; esophageal square cell carcinoma; gastric adenocarcinoma; methylation; methylation-specific polymerase chain reaction