title: Holographic imaging of unlabelled sperm cells for semen analysis: a review.
abstract: Male reproductive health in both humans and animals is an important research field in biological study. In order to characterize the morphology, the motility and the concentration of the sperm cells, which are the most important parameters to feature them, digital holography demonstrated to be an attractive technique. Indeed, it is a label-free, non-invasive and high-resolution method that enables the characterization of live specimen. The review is intended both for summarizing the state-of-art on the semen analysis and recent achievement obtained by means of digital holography and for exploring new possible applications of digital holography in this field. Quantitative phase maps of living swimming spermatozoa.
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples - National Research Council, Naples, 80121, Italy. dicaprio@rowland.harvard.edu.|Rowland Institute at Harvard, Harvard University, Cambridge, MA, 02142, USA. dicaprio@rowland.harvard.edu.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Caprio
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples - National Research Council, Naples, 80121, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Ferrara
authors.affiliation: Institute "E. Caianiello" - National Research Council, Pozzuoli, 80078, Italy.
authors.forename: Lisa
authors.initials: L
authors.lastname: Miccio
authors.affiliation: Institute "E. Caianiello" - National Research Council, Pozzuoli, 80078, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Merola
authors.affiliation: Institute "E. Caianiello" - National Research Council, Pozzuoli, 80078, Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Memmolo
authors.affiliation: Institute "E. Caianiello" - National Research Council, Pozzuoli, 80078, Italy.
authors.forename: Pietro
authors.initials: P
authors.lastname: Ferraro
authors.affiliation: Institute for Microelectronics and Microsystems, Unit of Naples - National Research Council, Naples, 80121, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Coppola
mesh_terms: D000818:Animals; D002465:Cell Movement; D058948:Cell Tracking; D006696:Holography / Q000379:methods*; D006801:Humans; D008297:Male; D055101:Semen Analysis / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology*
keywords: fertility; in vitro fertilization; quantitative phase microscopy; single cell analysis