title: Ecdysone regulates morphogenesis and function of Malpighian tubules in Drosophila melanogaster through EcR-B2 isoform.
abstract: Malpighian tubules are the osmoregulatory and detoxifying organs of Drosophila and its proper development is critical for the survival of the organism. They are made up of two major cell types, the ectodermal principal cells and mesodermal stellate cells. The principal and stellate cells are structurally and physiologically distinct from each other, but coordinate together for production of isotonic fluid. Proper integration of these cells during the course of development is an important pre-requisite for the proper functioning of the tubules. We have conclusively determined an essential role of ecdysone hormone in the development and function of Malpighian tubules. Disruption of ecdysone signaling interferes with the organization of principal and stellate cells resulting in malformed tubules and early larval lethality. Abnormalities include reduction in the number of cells and the clustering of cells rather than their arrangement in characteristic wild type pattern. Organization of F-actin and β-tubulin also show aberrant distribution pattern. Malformed tubules show reduced uric acid deposition and altered expression of Na(+)/K(+)-ATPase pump. B2 isoform of ecdysone receptor is critical for the development of Malpighian tubules and is expressed from early stages of its development.
authors.affiliation: Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005,Uttar Pradesh, India; Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow 226007, Uttar Pradesh, India.
authors.forename: Naveen Kumar
authors.initials: NK
authors.lastname: Gautam
authors.affiliation: Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005,Uttar Pradesh, India.
authors.forename: Puja
authors.initials: P
authors.lastname: Verma
authors.affiliation: Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005,Uttar Pradesh, India. Electronic address: madhu@bhu.ac.in.
authors.forename: Madhu G
authors.initials: MG
authors.lastname: Tapadia
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002452:Cell Count; D003599:Cytoskeleton / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000196:embryology* / Q000378:metabolism*; D004440:Ecdysone / Q000494:pharmacology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008317:Malpighian Tubules / Q000002:abnormalities / Q000196:embryology* / Q000201:enzymology / Q000473:pathology; D009024:Morphogenesis / Q000187:drug effects*; D009154:Mutation; D010641:Phenotype; D020033:Protein Isoforms / Q000378:metabolism; D011987:Receptors, Steroid / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism
chemical_list: D000199:Actins; D020033:Protein Isoforms; D011987:Receptors, Steroid; D014404:Tubulin; C055469:ecdysone receptor; D014527:Uric Acid; D004440:Ecdysone; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Drosophila melanogaster; Ecdysone receptor; Malpighian tubules; Na(+)/K(+) ATPase; Uric acid