title: Exogenous stimulation with Eclipta alba promotes hair matrix keratinocyte proliferation and downregulates TGF-β1 expression in nude mice.
abstract: Eclipta alba (L.) Hassk (E. alba) is a traditionally acclaimed medicinal herb used for the promotion of hair growth. However, to the best of our knowledge, no report has been issued to date on its effects on genetically distorted hair follicles (HFs). In this study, we aimed to identify an agent (stimuli) that may be beneficial for the restoration of human hair loss and which may be used as an alternative to synthetic drugs. We investigated the effects of petroleum ether extract (PEE) and different solvent fractions of E. alba on HFs of nude mice. Treatment was performed by topical application on the backs of nude mice and the changes in hair growth patterns were evaluated. Histological analysis was carried out to evaluate the HF morphology and the structural differences. Immunohistochemical (IHC) staining was performed to visualize follicular keratinocyte proliferation. The histological assessments revealed that the PEE-treated skin specimens exhibited prominent follicular hypertrophy. Subsequently, IHC staining revealed a significant increase (p<0.001) in the number of follicular keratinocytes in basal epidermal and matrix cells. Our results also demonstrated that PEE significantly (p<0.001) reduced the levels of transforming growth factor-β1 (TGF-β1) expression during early anagen and anagen-catagen transition. Our results suggest that PEE of E. alba acts as an important exogenous mediator that stimulates follicular keratinocyte proliferation and delays terminal differentiation by downregulating TGF-β1 expression. Thus, this study highlights the potential use of PEE of E. alba in the treatment of certain types of alopecia.
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Begum
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Mi Ra
authors.initials: MR
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Li Juan
authors.initials: LJ
authors.lastname: Gu
authors.affiliation: Molecular Genetics and Genomics Laboratory, Department of Horticulture, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Jamil
authors.initials: J
authors.lastname: Hossain
authors.affiliation: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
authors.forename: Chang Keun
authors.initials: CK
authors.lastname: Sung
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D036601:Eclipta / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis*
chemical_list: D010936:Plant Extracts; C508961:Tgfb1 protein, mouse; D053773:Transforming Growth Factor beta1