title: Osteogenesis of human adipose-derived stem cells on hydroxyapatite-mineralized poly(lactic acid) nanofiber sheets.
abstract: Electrospun fiber sheets with various orientations (random, partially aligned, and aligned) and smooth and roughened casted membranes were prepared. Hydroxyapatite (HA) crystals were in situ formed on these material surfaces via immersion in 10× simulated body fluid solution. The size and morphology of the resulting fibers were examined using scanning electron microscopy. The average diameter of the fibers ranged from 225±25 to 1050±150 nm depending on the electrospinning parameters. Biological experiment results show that human adipose-derived stem cells exhibit different adhesion and osteogenic differentiation on the three types of fiber. The cell proliferation and osteogenic differentiation were best on the aligned fibers. Similar results were found for phosphorylated focal adhesion kinase expression. Electrospun poly(lactic acid) aligned fibers mineralized with HA crystals provide a good environment for cell growth and osteogenic differentiation and thus have great potential in the tissue engineering field.
authors.affiliation: Department of Health Developing and Health Marketing, Kainan University, Taiwan.
authors.forename: Fu-Chen
authors.initials: FC
authors.lastname: Kung
authors.affiliation: Department of Chemical and Materials Engineering, Tunghai University, Taiwan. Electronic address: chichang31@thu.edu.tw.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Graduate Institute of Clinical Medicine, Taipei Medical University, Taiwan. Electronic address: Laitw@tmu.edu.tw.
authors.forename: Wen-Fu T
authors.initials: WF
authors.lastname: Lai
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry* / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013818:Thermogravimetry
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D015675:Osteocalcin; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite; D051417:Focal Adhesion Kinase 1
keywords: Bone tissue; Electrospun; Human adipose-derived stem cells; Mineralization; Simulated body fluid