title: Amelioration of lesions associated with 24-hour suboptimal platelet storage at 16 °C by a p38MAPK inhibitor, VX-702.
abstract: OBJECTIVE
  Previous studies with p38MAPK inhibitors at room temperature demonstrated that they improve a large number of platelet storage parameters, but cannot substantially inhibit p38MAPK activation nor protect against widespread decrements in platelet quality parameters during 4 °C storage. In this study, platelet quality parameters and inhibition of p38MAPK by VX-702 were studied after incubation of platelets at 16 °C without agitation, suboptimal storage conditions which produce moderate platelet decrements.
  
  
  METHODS
  Trima apheresis units were collected and aliquoted into three 60-ml CLX storage bags: (i) a control aliquot which was held at 20-24 °C with constant agitation; (ii) a test aliquot which was held at 20-24 °C with agitation until Day 2, when it was reincubated at 16 ± 1 °C for 24 ± 0·5 h without agitation and then returned 20-24 °C with agitation; (iii) a test aliquot containing 1 μm VX-702 stored in an identical fashion as aliquot 2. Aliquots were tested for an array of platelet storage parameters and p38MAPK activation on Days 1, 4 and 7.
  
  
  RESULTS
  Many platelet storage parameters and p38MAPK activation were adversely affected by 24-h incubation at 16 °C without agitation. With the exception of ESC, addition of VX-702 prevented p38MAPK activation and the decrements in most observed parameters.
  
  
  CONCLUSIONS
  Unlike 4 °C storage, VX-702 prevents activation of p38MAPK and decrements in many platelet storage parameters after exposure to 16 °C without agitation for 24 h.
authors.affiliation: Blood Components Department, American Red Cross Holland Laboratory, Rockville, MD, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Wagner
authors.forename: A
authors.initials: A
authors.lastname: Skripchenko
authors.forename: S
authors.initials: S
authors.lastname: Seetharaman
authors.forename: J
authors.initials: J
authors.lastname: Kurtz
mesh_terms: D001792:Blood Platelets / Q000187:drug effects*; D001793:Blood Preservation / Q000379:methods*; D003080:Cold Temperature; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D010671:Phenylurea Compounds / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D010671:Phenylurea Compounds; C517771:VX 702; D048051:p38 Mitogen-Activated Protein Kinases
keywords: apheresis; platelet components; platelet function