title: Visualizing tributyltin (TBT) in bacterial aggregates by specific rhodamine-based fluorescent probes.
abstract: Here we present the first examples of fluorescent and colorimetric probes for microscopic TBT imaging. The fluorescent probes are highly selective and sensitive to TBT and have successfully been applied for imaging of TBT in bacterial Rhodobacter ferrooxidans sp. strain SW2 cell-EPS-mineral aggregates and in cell suspensions of the marine cyanobacterium Synechococcus PCC 7002 by using confocal laser scanning microscopy.
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors.forename: Xilang
authors.initials: X
authors.lastname: Jin
authors.affiliation: Center for Applied Geosciences Eberhard Karls University Tübingen, Hölderlinstr. 12, 72074 Tübingen, Germany.
authors.forename: Likai
authors.initials: L
authors.lastname: Hao
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: She
authors.affiliation: Center for Applied Geosciences Eberhard Karls University Tübingen, Hölderlinstr. 12, 72074 Tübingen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Obst
authors.affiliation: Center for Applied Geosciences Eberhard Karls University Tübingen, Hölderlinstr. 12, 72074 Tübingen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kappler
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China; College of Chemistry, Beijing Normal University, No. 19, Xinjiekouwai Street, Haidian District, Beijing 100875, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yin
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China. Electronic address: lijianli@nwu.edu.cn.
authors.forename: Jianli
authors.initials: J
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors.forename: Lanying
authors.initials: L
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Shi
mesh_terms: D003124:Colorimetry; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D008958:Models, Molecular; D008968:Molecular Conformation; D061848:Optical Imaging; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry*; D016992:Rhodobacter / Q000166:cytology / Q000378:metabolism*; D012545:Schiff Bases / Q000737:chemistry; D046940:Synechococcus / Q000166:cytology / Q000378:metabolism*; D014220:Trialkyltin Compounds / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012545:Schiff Bases; D014220:Trialkyltin Compounds; C011559:tributyltin
keywords: Fluorescence imaging; Fluorescent probes; Mapping TBT distribution; Rhodamine; Tributyltin (TBT)