title: Interleukin/chitosan (JY) adjuvant enhances the mucosal immunity of human papillomavirus 16 L1 virus-like particles in mice.
abstract: Mucosal immunity may provide a defense against human papillomavirus (HPV) but there are no FDA-approved adjuvants capable of stimulating immune responses within mucosal tissues. After mice were immunized intranasally three times with HPV16 L1 virus-like particles plus with JY adjuvant, which is composed of interleukin-2 and chitosan, sera IgG antibody titer, sera neutralizing antibody titer, sIgA concentration in respiratory tract washes, sIgA concentration in vaginal washes and the number of spot-forming cells (SFC) in splenic lymphocytes were 320 ± 15, 40 ± 2, 27 ± 1.3, 27 ± 1.7 μg/ml and 176.7 ± 6 SFC/10(6), respectively; In the group without JY adjuvant, the outcomes were 80 ± 9.4, null, 22 ± 1, 20 ± 2.4 μg/ml and 91 ± 5.2 SFC/10(6), respectively. Therefore, JY adjuvant may be an effective mucosal adjuvant for HPV vaccine in mice.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Yingxin Street 100, Beijing, 100052, China, mfl0729@126.com.
authors.forename: Fenlian
authors.initials: F
authors.lastname: Ma
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Lishu
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000281:Administration, Intranasal; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D048271:Chitosan / Q000008:administration & dosage*; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology*; D018928:Immunity, Mucosal; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D007376:Interleukin-2 / Q000008:administration & dosage*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D012137:Respiratory System / Q000276:immunology; D013154:Spleen / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000276:immunology*; D014621:Vagina / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007376:Interleukin-2; D058425:Vaccines, Virus-Like Particle; D048271:Chitosan