title: Affinity composite cryogel discs functionalized with Reactive Red 120 and Green HE 4BD dye ligands: application on the separation of human immunoglobulin G subclasses.
abstract: Naturally produced by the human immune system, immunoglobulin nowadays is widely used for in vivo and in vitro purposes. The increased needs for pure immunoglobulin have prompted researchers to find new immunoglobulin chromatographic separation processes. Cryogels as chromatographic adsorbents, congregate several mechanical features including good compatibility, large pore structure, flexibility, short diffusion pathway and stability. These different characteristics make them a good alternative to conventional chromatographic methods and allowing their potential use in separation technology. In the present study, two sets of poly(2-hydroxyethyl methacrylate) (PHEMA) based beads were prepared and functionalized with Reactive Red 120 (RR) and Reactive Green HE 4BD (RG) dyes, and then embedded into supermacroporous cryogels. The morphology, physical and chemical features of the prepared bead embedded composite cryogel discs (CCDs) were performed by scanning electron microscopy (SEM), swelling test, elemental analysis and Fourier transform infrared spectroscopy (FTIR). The results showed that the embedded composite cryogel discs have a specific surface area of 192.0 m(2)/g with maximum adsorption capacity of HIgG 239.8 mg/g for the RR functionalized CCD and 170 mg/g for RG functionalized CCD columns, both at pH 6.2.
authors.affiliation: Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
authors.forename: Sabina
authors.initials: S
authors.lastname: Huseynli
authors.affiliation: Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
authors.forename: Gözde
authors.initials: G
authors.lastname: Baydemir
authors.affiliation: Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Sarı
authors.affiliation: Laboraory of "Valorisation des Ressources Naturelles et Produits de Santé (VRNPS)", Doctoral School of Sciences and Technology, Lebanese University, Rafic Hariri University Campus, Hadath, Lebanon.
authors.forename: Assem
authors.initials: A
authors.lastname: Elkak
authors.affiliation: Department of Chemistry, Biochemistry Division, Hacettepe University, Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002846:Chromatography, Affinity / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry; D014227:Triazines / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D059985:Cryogels; D007074:Immunoglobulin G; D014227:Triazines; D011102:Polyhydroxyethyl Methacrylate; C026268:procion green H-E4BD; C042516:reactive red 120 dye
keywords: Composite cryogels; Dye affinity chromatography; HIgG subclasses; PHEMA beads