title: Downregulation of striatin leads to hyperphosphorylation of MAP2, induces depolymerization of microtubules and inhibits proliferation of HEK293T cells.
abstract: Microtubules are tubular polymers of α/β-tubulin that are involved in the maintenance of cell shape, motility, and intracellular transport and in the segregation of chromosomes during cell division. Microtubules are dynamic structures, and their assembly is regulated by phosphoproteins called microtubule-associated proteins (MAPs). We propose that striatin, a protein belonging to the striatin family of proteins, is involved in regulation of microtubules. In HEK293T cells, striatin colocalizes with microtubules and stably associates with PP2Ac. Inhibition of striatin expression results in hyperphosphorylation of MAP2 and destabilizes microtubules. Striatin-induced destabilization of microtubules inhibited the proliferation of HEK293T cells and caused the accumulation of cells in the G0/G1 phase of the cell cycle. These results suggest that the PP2A/striatin complex modulates microtubule dynamics by regulating MAP2 phosphorylation.
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza St., 90-363 Lodz, Poland. Electronic address: julia@cbmm.lodz.pl.
authors.forename: Julia
authors.initials: J
authors.lastname: Kaźmierczak-Barańska
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza St., 90-363 Lodz, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Pęczek
authors.affiliation: Institute for Medical Biology of the Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland. Electronic address: pprzygodzka@cbm.pan.pl.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Przygodzka
authors.affiliation: Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza St., 90-363 Lodz, Poland. Electronic address: marcin@cbmm.lodz.pl.
authors.forename: Marcin J
authors.initials: MJ
authors.lastname: Cieślak
mesh_terms: D002148:Calmodulin-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D049109:Cell Proliferation; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D002148:Calmodulin-Binding Proteins; C489788:MAP2 protein, human; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C491925:STRN protein, human
keywords: MAP2; Microtubule; Phosphorylation; Proliferation; Protein phosphatase 2A; Striatin