title: Effect of Lactobacillus acidophilus NS1 on plasma cholesterol levels in diet-induced obese mice.
abstract: We investigated the probiotic properties of Lactobacillus acidophilus NS1, such as acid resistance, bile tolerance, adherence to HT-29 cells, and cholesterol assimilation activity. In an animal study, 7-wk-old male C57BL/6 mice were fed a normal diet, a high-fat diet (HFD), or an HFD with L. acidophilus NS1 (ca. 1.0×10(8) cfu/mL) for 10 wk. Total cholesterol and low-density lipoprotein (LDL) cholesterol levels were significantly lower in mice fed an HFD with L. acidophilus NS1 than in those fed an HFD only, whereas high-density lipoprotein cholesterol levels were similar between these 2 groups. To understand the mechanism of the cholesterol-lowering effect of L. acidophilus NS1 on the HFD-mediated increase in plasma cholesterol levels, we determined mRNA levels of genes involved in cholesterol homeostasis in the liver. Expression of sterol regulatory element-binding protein 2 (Srebp2) and LDL receptor (Ldlr) in the liver was dramatically reduced in mice fed a HFD compared with those fed a normal diet. When L. acidophilus NS1 was administered orally to HFD-fed mice, an HFD-induced suppression of Srebp2 and Ldlr expression in the liver was abolished. These results suggest that the oral administration of L. acidophilus NS1 to mice fed an HFD increased the expression of Srebp2 and Ldlr in the liver, which was inhibited by high fat intake, thus leading to a decrease in plasma cholesterol levels. Lactobacillus acidophilus NS1 could be a useful probiotic microorganism for cholesterol-lowering dairy products and the improvement of hyperlipidemia and hepatic lipid metabolism.
authors.affiliation: Division of Animal Science, and Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: M
authors.initials: M
authors.lastname: Song
authors.affiliation: Department of Biological Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.affiliation: Research & Business Development Center, Nong Shim Co. Ltd., 112 Yeouidaebang-ro, Seoul, 156-709, Republic of Korea.
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.affiliation: Research & Business Development Center, Nong Shim Co. Ltd., 112 Yeouidaebang-ro, Seoul, 156-709, Republic of Korea.
authors.forename: B
authors.initials: B
authors.lastname: Min
authors.affiliation: Research & Business Development Center, Nong Shim Co. Ltd., 112 Yeouidaebang-ro, Seoul, 156-709, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Jung
authors.affiliation: Research & Business Development Center, Nong Shim Co. Ltd., 112 Yeouidaebang-ro, Seoul, 156-709, Republic of Korea.
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea. Electronic address: ekim@chonnam.ac.kr.
authors.forename: E
authors.initials: E
authors.lastname: Kim
authors.affiliation: Division of Animal Science, and Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 500-757, Republic of Korea. Electronic address: soh@chonnam.ac.kr.
authors.forename: S
authors.initials: S
authors.lastname: Oh
mesh_terms: D000818:Animals; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood*; D059305:Diet, High-Fat / Q000009:adverse effects*; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000209:etiology / Q000382:microbiology; D019936:Probiotics / Q000008:administration & dosage; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D012336:RNA, Ribosomal, 16S; D011973:Receptors, LDL; C497391:Srebf2 protein, mouse; D051782:Sterol Regulatory Element Binding Protein 2; D014280:Triglycerides
keywords: Lactobacillus acidophilus; cholesterol; obesity; probiotic