title: Micro-CT evaluation of high pressure-decellularized cardiovascular tissues transplanted in rat subcutaneous accelerated-calcification model.
abstract: We have succeeded in reducing the calcification of acellular aortas or valves in porcine allogeneic system by removing the DNA and phospholipids, but its further reduction is desirable. Here, the calcification of the acellular tissue was evaluated in rat subcutaneous transplantation model which is known as calcification model. Acellular samples prepared by high-hydrostatic pressure (HHP) protocols with different washing media were implanted and the calcification was monitored under micro-computed tomography for 1 and 3 months. The amount of the calcium deposition was quantitatively evaluated by atomic absorption spectroscopy. A cell culture medium showed very good cell removal ability but led to severe calcification at 1 month, and surprisingly the calcium deposition increased as the washing period increased. This calcification was suppressed by removing the DNA fraction with high DNase concentration. On the other hand, the calcification was greatly reduced when washed with saline even at low DNase concentration after 2 weeks washing. These results suggest that the ion species in the washing medium and the residual DNase cooperatively affect the tendency of in vivo calcification, which led us to the possibility of reduced calcification of acellular cardiac tissues.
authors.affiliation: Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Mahara
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Sago
authors.forename: Haruka
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Tomo
authors.initials: T
authors.lastname: Ehashi
authors.forename: Kenji
authors.initials: K
authors.lastname: Minatoya
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tanaka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakatani
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Moritan
authors.forename: Toshiya
authors.initials: T
authors.lastname: Fujisato
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamaoka
mesh_terms: D000818:Animals; D001011:Aorta / Q000637:transplantation*; D002114:Calcinosis; D004195:Disease Models, Animal; D006874:Hydrostatic Pressure; D051381:Rats; D040521:Subcutaneous Tissue / Q000000981:diagnostic imaging* / Q000601:surgery*; D013552:Swine; D061205:Vascular Calcification / Q000000981:diagnostic imaging* / Q000209:etiology*; D055114:X-Ray Microtomography