title: Antifibrogenic effects of B16 melanoma-conditioned medium.
abstract: BACKGROUND
  Some malignant cancers show high levels of local invasiveness by the secretion of soluble factors that can degrade adjacent tissues and suppress surrounding cell growth. We investigated the possibility of treating fibroproliferative scars based on these properties of malignant melanoma.
  
  
  METHODS
  B16 melanoma-conditioned medium (B16 M-CM) was added to keloid fibroblasts (KFs), and proliferation, migration, and type I collagen production were measured. The cell cycle and signaling pathways were also analyzed. Proteins associated with cell proliferation were measured with Western blot analysis. Animal experiments using a rabbit ear model was performed to confirm the effect of B16 M-CM in vivo.
  
  
  RESULTS
  B16 M-CM reduced proliferation, migration, and type I collagen production of KFs. This treatment also increased the number of cells in the subG1 phase and decreased phosphorylation levels of AKT, extracellular signal-regulated kinase1/2, cyclin D1, and c-Myc of KFs. Additionally, B16 M-CM reduced the thickness of rabbit ear scars in the rabbit ear model in vivo.
  
  
  CONCLUSIONS
  B16 M-CM can suppress proliferation, migration, and type I collagen production of KFs. In addition, concentrated B16 M-CM reduced scar thickness in the rabbit ear model. The specific proteins involved should be identified in a future study.
authors.affiliation: Department of Plastic and Reconstructive Surgery, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam-si, Gyeonggi-do, Republic of Korea.
authors.forename: Chung Hun
authors.initials: CH
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Yonsei University, Incheon, Republic of Korea.
authors.forename: Wang Kyun
authors.initials: WK
authors.lastname: Kim
authors.affiliation: Department of Pharmacy, College of Pharmacy, Yonsei University, Incheon, Republic of Korea.
authors.forename: Chengjin
authors.initials: C
authors.lastname: Li
authors.affiliation: Dodream Plastic Surgery Clinic, Seoul, Republic of Korea.
authors.forename: Jea Yong
authors.initials: JY
authors.lastname: Song
authors.affiliation: Department of Pharmacy, College of Pharmacy, Yonsei University, Incheon, Republic of Korea.
authors.forename: Jong Hyuk
authors.initials: JH
authors.lastname: Sung
authors.affiliation: Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine, Seoul, Republic of Korea. Electronic address: iceberg554@naver.com.
authors.forename: Seung Yong
authors.initials: SY
authors.lastname: Song
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects*; D007627:Keloid / Q000517:prevention & control*; D008546:Melanoma, Experimental / Q000737:chemistry*; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011817:Rabbits
chemical_list: D017077:Culture Media, Conditioned; D003094:Collagen; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Hypertrophic scar; Keloid; Melanoma; Scar