title: Exploring the origin of the D genome of oat by fluorescence in situ hybridization.
abstract: Further understanding of the origin of cultivated oat would accelerate its genetic improvement. In particular, it would be useful to clarify which diploid progenitor contributed the D genome of this allohexaploid species. In this study, we demonstrate that the landmarks produced by fluorescence in situ hybridization (FISH) of species of Avena using probes derived from Avena sativa can be used to explore the origin of the D genome. Selected sets of probes were hybridized in several sequential experiments performed on exactly the same chromosome spreads, with multiple probes of cytological preparations. Probes pITS and A3-19 showed there might be a similar distribution of pITS between the Ac and D genomes. These results indicated that the Ac genome is closely related to the D genome, and that Avena canariensis (AcAc) could be the D-genome donor of cultivated oat.
authors.affiliation: a Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Luo
authors.forename: Haiqin
authors.initials: H
authors.lastname: Zhang
authors.forename: Houyang
authors.initials: H
authors.lastname: Kang
authors.forename: Xing
authors.initials: X
authors.lastname: Fan
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Lina
authors.initials: L
authors.lastname: Sha
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D018554:Avena / Q000235:genetics*; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase
keywords: Avena canariensis; D genome; FISH; avoine hexaploïde; génome D; hexaploid oat; origin; origine