title: PHBVHHx scaffolds loaded with umbilical cord-derived mesenchymal stem cells or hepatocyte-like cells differentiated from these cells for liver tissue engineering.
abstract: More attention has recently been focused on the treatment of various kinds of hepatic diseases based on cell-based therapies. In this study, mesenchymal stem cells were isolated from umbilical cord (UC-MSCs). Results confirmed that UC-MSCs could differentiate into adipocytes, osteoblasts and hepatocytes. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) (PHBVHHx), a new member of polyhydroxyalkanoate (PHA) family, was produced by bacteria. Liver-injured mouse model was established by CCl4 injection. PHBVHHx scaffolds were transplanted into the liver-injured mice. Liver morphology on day 28 post-transplantation of scaffolds loaded with UC-MSCs or hepatocyte-like cells differentiated from UC-MSCs significantly improved and looked similar to the normal liver. Concentrations of albumin (ALB) significantly increased, and total bilirubin (TB) and alanine axminotransferase (ALT) significantly decreased on days 14 and 28 post-transplantation of scaffolds loaded with UC-MSCs or differentiated UC-MSCs. HE staining showed that on day 28 post-transplantation of scaffolds loaded with UC-MSCs or differentiated UC-MSCs, livers had similar tissue structure of normal livers. Masson staining showed that on day 28 post-transplantation of scaffolds loaded with UC-MSCs or differentiated UC-MSCs, livers had less blue staining for collagen deposition compared with the others. These results demonstrated that PHBVHHx scaffolds loaded with UC-MSCs or differentiated UC-MSCs had the similar effect on injured livers and significantly promoted the recovery of injured livers.
authors.affiliation: Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Zhongchun
authors.initials: Z
authors.lastname: Su
authors.affiliation: Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Pengshan
authors.initials: P
authors.lastname: Li
authors.affiliation: Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Bogang
authors.initials: B
authors.lastname: Wu
authors.affiliation: Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Medicine, Jinan University, Guangzhou 510632, China.
authors.forename: Gexiu
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Huilan
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Department of Bone, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Biomedicine, College of Life Science and Technology, National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou 510632, China. Electronic address: wei70@hotmail.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000418:Albumins / Q000378:metabolism; D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D002454:Cell Differentiation; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000166:cytology*; D008099:Liver / Q000502:physiology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology; D011108:Polymers / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014470:Umbilical Cord / Q000166:cytology; D000094122:Polyhydroxybutyrates
chemical_list: D000418:Albumins; D001663:Bilirubin; D011108:Polymers; C435024:poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates
keywords: Mesenchymal stem cells; Scaffolds; Tissue engineering