title: Dexamethasone loaded core-shell SF/PEO nanofibers via green electrospinning reduced endothelial cells inflammatory damage.
abstract: Silk fibroin (SF)/PEO nanofibers prepared by green electrospinning is safe, non-toxic and environment friendly, it is a potential drug delivery carrier for tissue engineering. In this study, a core-shell nanofibers named as Dex@SF/PEO were obtained by green electrospinning with SF/PEO as the shell and dexamethasone (Dex) in the core. The nanofiber morphology and core-shell structure were studied by Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM). The Dex release behavior from the nanofibers was tested by High Performance liquid (HPLC) method. The protective effect of drug loaded nanofibers mats on Porcine hip artery endothelial cells (PIECs) against LPS-induced inflammatory damage were determined by MTT assay. TEM result showed the distinct core-shell structure of nanofibers. In vitro drug release studies demonstrated that dexamethasone can sustain release over 192 h and core-shell nanofibers showed more slow release of Dex compared with the blending electrospinning nanofibers. Anti-inflammatory activity in vitro showed that released Dex can reduce the PIECs inflammatory damage and apoptosis which induced by lipopolysaccharide (LPS). Dex@SF/PEO nanofibers are safe and non-toxic because of no harmful organic solvents used in the preparation, it is a promising environment friendly drug carrier for tissue engineering.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; Engineering Research Center of Technical Textiles, Ministry of Education, College of Textiles, Donghua University, Shanghai, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ei-Shanshory
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt. Electronic address: melnewehy@ksu.edu.sa.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Newehy
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt.
authors.forename: Hany A
authors.initials: HA
authors.lastname: Ei-Hamshary
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Salem S
authors.initials: SS
authors.lastname: Al-Deyab
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
authors.forename: Chuanglong
authors.initials: C
authors.lastname: He
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Electronic address: xmm@dhu.edu.cn.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D003907:Dexamethasone / Q000737:chemistry / Q000494:pharmacology*; D055664:Electrochemical Techniques; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D057139:Nanofibers / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D047011:Silk / Q000737:chemistry; D023822:Tissue Engineering
chemical_list: D000893:Anti-Inflammatory Agents; D047011:Silk; D011092:Polyethylene Glycols; D003907:Dexamethasone; D005349:Fibroins
keywords: Control release; Dexamethasone; Electrospinning; Nanofibers