title: Pyroglutamic acid stimulates DNA synthesis in rat primary hepatocytes through the mitogen-activated protein kinase pathway.
abstract: We purified pyroglutamic acid from human placental extract and identified it as a potent stimulator of rat primary hepatocyte DNA synthesis. Pyroglutamic acid dose-dependently stimulated DNA synthesis, and this effect was inhibited by PD98059, a dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) inhibitor. Therefore, pyroglutamic acid stimulated DNA synthesis in rat primary hepatocytes via MAPK signaling.
authors.affiliation: a Japan Bio Products Co., Ltd. , Fukuoka , Japan.
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Inoue
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Okita
authors.forename: Andreia
authors.initials: A
authors.lastname: de Toledo
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miyazaki
authors.forename: Eiichi
authors.initials: E
authors.lastname: Hirano
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Morinaga
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D004247:DNA / Q000096:biosynthesis* / Q000187:drug effects; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D010920:Placenta / Q000737:chemistry; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000302:isolation & purification / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley
chemical_list: D005419:Flavonoids; D007093:Imidazoles; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D004247:DNA; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one; D011761:Pyrrolidonecarboxylic Acid
keywords: DNA synthesis; mitogen-activated protein kinase; placental extracts; pyroglutamic acid; rat primary hepatocyte