title: Structural characterization and gastroprotective property of a novel glucofructan from Allium ampeloprasum var. porrum.
abstract: A new polysaccharide with an estimated weight-average molar mass of 2.6×10(3) was isolated from Allium ampeloprasum var. porrum by hot water extraction, and purified by Sephacryl S-300 HR high-resolution chromatography. It was composed of D-fructose and D-glucose in 10:6 molar ratio, respectively. The structure of the glucofructan was investigated by chemical and spectroscopic methods, including methylation analysis, nuclear magnetic resonance, and electrospray mass spectrometry (ES-MS). The results permitted the structure of the glucofructan to be written as α-D-Glcp-(1→1)-β-D-Fruf-(2→1)-{[α-D-Glcp-(1→6)-β-D-Fruf-(2→6)]-β-D-Fruf-(2→1)}4-β-D-Fruf-(2↔1)-α-D-Glcp. Results of the present study indicated that this new glucofructan exhibited significant gastroprotective property, using in vivo experimental models.
authors.affiliation: Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, PO Box 68045, CEP 21941-971 Rio de Janeiro, Brazil.
authors.forename: Camila R A
authors.initials: CR
authors.lastname: Malafaia
authors.affiliation: Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, PO Box 68045, CEP 21941-971 Rio de Janeiro, Brazil.
authors.forename: Bernadete P
authors.initials: BP
authors.lastname: da Silva
authors.affiliation: Laboratório Multiusuário de Análises por Ressonância Magnética Nuclear, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, CEP 21941-902 Rio de Janeiro, Brazil.
authors.forename: Luzineide W
authors.initials: LW
authors.lastname: Tinoco
authors.affiliation: Laboratório de Química de Plantas Medicinais, Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, PO Box 68045, CEP 21941-971 Rio de Janeiro, Brazil. Electronic address: parente@pq.cnpq.br.
authors.forename: José P
authors.initials: JP
authors.lastname: Parente
mesh_terms: D000490:Allium; D000818:Animals; D002240:Carbohydrate Sequence; D019610:Cytoprotection / Q000187:drug effects*; D005630:Fructans / Q000737:chemistry* / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D013270:Stomach / Q000166:cytology* / Q000187:drug effects*
chemical_list: D005630:Fructans; C082843:glucofructan; D005947:Glucose
keywords: Alliaceae; Allium ampeloprasum var. porrum; Chemical structure; Gastroprotective property; Glucofructan; Polysaccharide