title: Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract.
abstract: We investigated the end processing and degradation of premature tRNAs in wheat germ extract (WGE), which led to the discovery of end protectors useful for stabilizing an in vitro transcript against various ribonucleases and thereby enhancing its apparent activity in WGE.
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Doi
mesh_terms: D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010936:Plant Extracts / Q000494:pharmacology*; D020871:RNA Stability / Q000187:drug effects*; D012333:RNA, Messenger / Q000737:chemistry; D012343:RNA, Transfer / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012333:RNA, Messenger; D012343:RNA, Transfer