title: [Genotypic characteristics of Mycobacterium avium subsp. hominissuis strains].
abstract: Mycobacterium avium are typical environmental, non-tuberculosis microorganisms that occasionally cause mycotuberculosis, an infectious disease in wild and domestic animals, birds, and humans. Here, we report the results of the first study on the genetic diversity of the Russian population of M. avium. A total of 85 M. avium subsp. hominissuis (MAH) clinical strains were isolated from patients (including 30 HIV-positive individuals) with mycobacteriosis in St. Petersburg, 2008-2011. The biochemical identification of the microorganisms was carried out using the PCR detection of the mobile elements IS901 and IS900, as well as of the polymorphism of restriction fragments of the hsp65 gene. The genetic diversity of the isolates was evaluated by VNTR typing based on eight variable-number tandem repeats (VNTRs) (292, X3, 25, 47, 3, 7, 10, and 32 [Thibault et al., 2007]). The MAH population studied was characterized by 15 VNTR types, including nine unique patterns and six clusters of isolates with identical eight-digit profiles. The largest clusters (22221128 and 24221128) included 45 (59.2%) and 15 (19.7%) isolates, respectively; the others contained from 2-7 strains. The strains of the cluster 2533112'8 possessed a truncated TR10 locus (allele 2'). Taking into account the absence of the epidemiological links between the patients and the fact that the infection was delivered from the environment, the high rate of clustering of MAH isolates can be explained by the low discriminatory power of the eight-locus VNTR-typing scheme (HGDI 0-0.61).
authors.forename: D A
authors.initials: DA
authors.lastname: Starkova
authors.forename: T F
authors.initials: TF
authors.lastname: Otten
authors.forename: I V
authors.initials: IV
authors.lastname: Mokrousov
authors.forename: A A
authors.initials: AA
authors.lastname: Viazovaia
authors.forename: B I
authors.initials: BI
authors.lastname: Vishnevskiĭ
authors.forename: O V
authors.initials: OV
authors.lastname: Narvskaia
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D018834:Chaperonin 60 / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D009162:Mycobacterium avium / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D012426:Russia
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; C074119:heat-shock protein 65, Mycobacterium