title: Effects of platelet-rich plasma, adipose-derived stem cells, and stromal vascular fraction on the survival of human transplanted adipose tissue.
abstract: Traditional adipose tissue transplantation has unpredictable viability and poor absorption rates. Recent studies have reported that treatment with platelet-rich plasma (PRP), adipose-derived stem cells (ASCs), and stromal vascular fraction (SVF) are related to increased survival of grafted adipose tissue. This study was the first simultaneous comparison of graft survival in combination with PRP, ASCs, and SVF. Adipose tissues were mixed with each other, injected subcutaneously into the back of nude mice, and evaluated at 4, 8, and 12 weeks. Human adipocytes were grossly maintained in the ASCs and SVF mixtures. Survival of the adipose tissues with PRP was observed at 4 weeks and with SVF at 8 and 12 weeks. At 12 weeks, volume reduction in the ASCs and SVF mixtures were 36.9% and 32.1%, respectively, which were significantly different from that of the control group without adjuvant treatment, 51.0%. Neovascular structures were rarely observed in any of the groups. Our results suggest that the technique of adding ASCs or SVF to transplanted adipose tissue might be more effective than the conventional grafting method. An autologous adipose tissue graft in combination with ASCs or SVF may potentially contribute to stabilization of engraftment.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Korea University College of Medicine, Seoul, Korea.
authors.forename: Deok-Yeol
authors.identifier: 0000-0001-6288-6597
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Medical Science Research Center, Ansan Hospital, Korea University Medical Center, Ansan, Korea.
authors.forename: Yi-Hwa
authors.identifier: 0000-0002-0556-2489
authors.initials: YH
authors.lastname: Ji
authors.affiliation: Department of Plastic and Reconstructive Surgery, Korea University College of Medicine, Seoul, Korea.
authors.forename: Deok-Woo
authors.identifier: 0000-0003-2422-9662
authors.initials: DW
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, Korea University College of Medicine, Seoul, Korea.
authors.forename: Eun-Sang
authors.identifier: 0000-0002-5695-3914
authors.initials: ES
authors.lastname: Dhong
authors.affiliation: Department of Plastic and Reconstructive Surgery, Korea University College of Medicine, Seoul, Korea.
authors.forename: Eul-Sik
authors.identifier: 0000-0001-5734-6625
authors.initials: ES
authors.lastname: Yoon
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000328:Adult; D000818:Animals; D005260:Female; D006085:Graft Survival; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053657:Platelet-Rich Plasma; D013234:Stem Cells; D017154:Stromal Cells / Q000637:transplantation*; D014183:Transplantation, Heterologous
keywords: Adipose Tissue; Platelet-Rich Plasma; Stem Cell; Tissue Graft