title: Programmed folding of DNA origami structures through single-molecule force control.
abstract: Despite the recent development in the design of DNA origami, its folding yet relies on thermal or chemical annealing methods. We here demonstrate mechanical folding of the DNA origami structure via a pathway that has not been accessible to thermal annealing. Using magnetic tweezers, we stretch a single scaffold DNA with mechanical tension to remove its secondary structures, followed by base pairing of the stretched DNA with staple strands. When the force is subsequently quenched, folding of the DNA nanostructure is completed through displacement between the bound staple strands. Each process in the mechanical folding is well defined and free from kinetic traps, enabling us to complete folding within 10 min. We also demonstrate parallel folding of DNA nanostructures through multiplexed manipulation of the scaffold DNAs. Our results suggest a path towards programmability of the folding pathway of DNA nanostructures.
authors.affiliation: 1] National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea [2] Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Wooli
authors.initials: W
authors.lastname: Bae
authors.affiliation: 1] National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea [2] Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Kipom
authors.initials: K
authors.lastname: Kim
authors.affiliation: 1] National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea [2] Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Duyoung
authors.initials: D
authors.lastname: Min
authors.affiliation: 1] National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea [2] Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Je-Kyung
authors.initials: JK
authors.lastname: Ryu
authors.affiliation: Korea Institute for Advanced Study, Seoul 130-722, South Korea.
authors.forename: Changbong
authors.initials: C
authors.lastname: Hyeon
authors.affiliation: 1] National Creative Research Initiative Center for Single-Molecule Systems Biology, KAIST, Daejeon 305-701, South Korea [2] Department of Physics, KAIST, Daejeon 305-701, South Korea.
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Yoon
mesh_terms: D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D059346:Magnets; D049329:Nanostructures; D036103:Nanotechnology / Q000379:methods*; D009690:Nucleic Acid Conformation
chemical_list: D004247:DNA