title: Nanostructured layer-by-layer films containing phaeophytin-b: electrochemical characterization for sensing purposes.
abstract: This paper reports the study and characterization of a new platform for practical applications, where the use of phaeophytin-b (phaeo-b), a compound derived from chlorophyll, was characterized and investigated for sensing purposes. Modified electrodes with nanostructured phaeo-b films were fabricated via the layer-by-layer (LbL) technique, where phaeo-b was assembled with cashew gum, a polysaccharide, or with poly(allylamine) hydrochloride (PAH). The multilayer formation was investigated with UV-Vis spectroscopy by monitoring the absorption band associated to phaeo-b at approximately 410 nm, where distinct molecular interactions between the materials were verified. The morphology of the films was analyzed by atomic force microscopy (AFM). The electrochemical properties through redox behavior of phaeo-b were studied with cyclic voltammetry. The produced films were applied as sensors for hydrogen peroxide (H2O2) detection. In terms of sensing, the cashew/phaeo-b film exhibited the most promising result, with a fast response and broad linear range upon the addition of H2O2. This approach provides a simple and inexpensive method for development of a nonenzymatic electrochemical sensor for H2O2.
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC 88040900, Brazil.
authors.forename: Gisele Elias Nunes
authors.initials: GE
authors.lastname: Pauli
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, Brazil.
authors.forename: Felipe B
authors.initials: FB
authors.lastname: Araruna
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, Brazil; Laboratório Interdisciplinar de Materiais Avançados, LIMAV, CCN, UFPI, Teresina, PI 64049-550, Brazil.
authors.forename: Carla
authors.initials: C
authors.lastname: Eiras
authors.affiliation: Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaíba, Brazil.
authors.forename: José Roberto S A
authors.initials: JR
authors.lastname: Leite
authors.affiliation: Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, 58051-970 João Pessoa, Paraíba, Brazil.
authors.forename: Otemberg Souza
authors.initials: OS
authors.lastname: Chaves
authors.affiliation: Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, 58051-970 João Pessoa, Paraíba, Brazil.
authors.forename: Severino Gonçalves
authors.initials: SG
authors.lastname: Brito Filho
authors.affiliation: Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Universidade Federal da Paraíba, 58051-970 João Pessoa, Paraíba, Brazil.
authors.forename: Maria de Fátima Vanderlei
authors.initials: Mde F
authors.lastname: de Souza
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC 88040900, Brazil.
authors.forename: Lucas Natálio
authors.initials: LN
authors.lastname: Chavero
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC 88040900, Brazil.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Sartorelli
authors.affiliation: Departamento de Física, Universidade Federal de Santa Catarina, Florianópolis, SC 88040900, Brazil. Electronic address: ivan.bechtold@ufsc.br.
authors.forename: Ivan H
authors.initials: IH
authors.lastname: Bechtold
mesh_terms: D002734:Chlorophyll / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006861:Hydrogen Peroxide / Q000737:chemistry; D018625:Microscopy, Atomic Force / Q000379:methods; D049329:Nanostructures / Q000737:chemistry*; D010084:Oxidation-Reduction; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D002734:Chlorophyll; D006861:Hydrogen Peroxide
keywords: Electrochemical sensor; LbL technique; Phaeophytin-b