title: Impairments of mecA gene detection in bovine Staphylococcus spp.
abstract: Staphylococcus aureus antimicrobial resistance, especially to beta-lactams, favors treatment failures and its persistence in herd environment. This work aimed to develop a more specific primer for mecA gene detection based on the comparison of the conserved regions from distinct host origins and also investigated the presence of homologue mecA(LGA251) in bovine strains. A total of 43 Staphylococcus spp. were included in this study, comprising 38 bovine S. aureus, two human and three equine coagulase-negative staphylococci (CNS). Phenotypical methicillin-resistance detection was performed through oxacillin agar-screening and cefoxitin disk-diffusion test. None isolate tested positive for mecA(LGA251) gene. For mecA gene PCR, new primers were designed based on the sequences of human S. aureus (HE681097) and bovine S. sciuri (AY820253) mecA. The new primers based on the S. aureus mecA sequence amplified fragments of human and equine CNS and the ones based on S. sciuri mecA sequence only yielded fragments for S. aureus bovine strains. Multiples alignments of mecA gene sequences from bovine, human and equine revealed punctual but significant differences in bovine strains that can lead to the mecA gene detection impairment. The observed divergences of mecA gene sequences are not a matter of animal or human origin, it is a specificity of bovine samples.
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Dayanne Araújo
authors.initials: DA
authors.lastname: de Melo
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Irene da Silva
authors.initials: Ida S
authors.lastname: Coelho
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Cássia Couto
authors.initials: CC
authors.lastname: da Motta
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Anna Carolina Coelho Marín
authors.initials: AC
authors.lastname: Rojas
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Felipe Carlos
authors.initials: FC
authors.lastname: Dubenczuk
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Shana de Mattos de Oliveira
authors.initials: Sde M
authors.lastname: Coelho
authors.affiliation: Departamento de Microbiologia e Imunologia Veterinária Universidade Federal Rural do Rio de Janeiro SeropédicaRJ Brazil Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil.
authors.forename: Miliane Moreira Soares
authors.initials: MM
authors.lastname: de Souza
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006736:Horses; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction / Q000379:methods; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013203:Staphylococcal Infections / Q000382:microbiology* / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus
keywords: Staphylococccus; antimicrobial resistance; bovine mastitis; mecA gene