title: Molecular characterization and phylogenetic analysis of the eukaryotic translation initiation factor 4A gene in Antheraea pernyi (Lepdoptera: Saturniidae).
abstract: Eukaryotic initiation factor 4A (eIF-4A) is an essential component for protein translation in eukaryotes. The eIF-4A gene (ApeIF-4A) was isolated and characterized from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae). The obtained cDNA sequence was 1,435-bp long with an open reading frame of 1,266 bp encoding 421 amino acids. The predicted amino acid sequence shared several conserved features as found in known eIF-4As and revealed 74 and 78% identities with eIF-4As of Homo sapiens L. and Drosophila melanogaster (Meigen), respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that ApeIF-4A was transcribed at four developmental stages and in all tissues tested, suggesting that it plays an important role in development of A. pernyi. Homologous alignment suggested that eIF-4As are highly conserved throughout evolution of eukaryote organisms. Phylogenetic trees based on the amino acid and nucleotide sequences of eIF-4A demonstrated a similar topology with the classical systematics, suggesting that it has the potential value in phylogenetic inference of eukaryotes.
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China *These authors contributed equally to this work.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China Key Laboratory of Wild Silkworms of Liaoning Province, Sericultural Institute of Liaoning Province, Fengcheng 118100, China *These authors contributed equally to this work.
authors.forename: Yan-Qun
authors.initials: YQ
authors.lastname: Liu
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yao
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Run-Xi
authors.initials: RX
authors.lastname: Xia
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China liqunmuzi2000@163.com.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics* / Q000378:metabolism; D020224:Expressed Sequence Tags; D005260:Female; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000502:physiology*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D019476:Insect Proteins; D039601:Eukaryotic Initiation Factor-4A
keywords: Antheraea pernyi; eukaryotic initiation factor 4A; expression pattern; phylogenetic inference