title: Influence of porcine-derived collagen matrix on endothelial progenitor cells: an in vitro study.
abstract: Porcine-derived collagen matrix (PDCM) has been reported as a promising alternative to autogenous soft tissue grafts in periodontal plastic surgery. The aim of this study was to analyze the influence of a novel PDCM on endothelial progenitor cells (EPC) in vitro. EPC were isolated from human peripheral blood, cultured and transferred on the PDCM (mucoderm®). Tissue culture polystyrene surface (TCPS) served as control. Cell viability of EPC on PDCM was measured by a MTT and PrestoBlue® assay. Migration ability was tested using a Boyden migration assay. A ToxiLight® assay was performed to analyze the influence of PDCM on adenylate kinase (ADK) release and apoptosis rate of EPC. Using the MTT assay, EPC cultured on PDCM demonstrated a significantly increased cell viability compared to the control group at days 3, 6 and 12 (p each <0.001). According to the PrestoBlue® assay, EPC showed a significant increase of cell viability compared to the control group at 48, 72, and 96 h (p each <0.001). In the Boyden migration assay, a significantly increased EPC migration ability could be observed after 3-12 days (p each ≤0.001). No significantly increased apoptosis rate of EPC on PDCM could be observed with exception after 96 h (p each >0.05). Overall, our results suggest a good biocompatibility of PDCM without any cytotoxic effects on EPC, which might support a rapid revascularization and therefore a sufficient ingrowth of the PDCM.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
authors.forename: Andreas Max
authors.initials: AM
authors.lastname: Pabst
authors.affiliation: Department of Prosthetic Dentistry, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
authors.forename: Karl-Martin
authors.initials: KM
authors.lastname: Lehmann
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Walter
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Krüger
authors.affiliation: Department of Periodontology, Victor Babes University of Medicine and Pharmacology, Piata E. Murgu 2, 300041, Timisoara, Romania.
authors.forename: Stefan-Ioan
authors.initials: SI
authors.lastname: Stratul
authors.affiliation: Department of Operative Dentistry and Periodontology, University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany. Kasaj@gmx.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Kasaj
mesh_terms: D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000502:physiology*; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
chemical_list: D003094:Collagen
keywords: Collagen matrix; EPC; Porcine acellular dermis; Revascularization; Tissue regeneration