title: Serological markers of enterocyte damage and apoptosis in patients with celiac disease, autoimmune diabetes mellitus and diabetes mellitus type 2.
abstract: Impairment of mucosal barrier integrity of small intestine might be causative in immune-mediated gastrointestinal diseases. We tested the markers of epithelial apoptosis - cytokeratin 18 caspase-cleaved fragment (cCK-18), and enterocyte damage - intestinal fatty acid-binding protein (I-FABP) and soluble CD14 (sCD14) in sera of patients with untreated celiac disease (CLD), those on gluten-free diet (CLD-GFD), patients with autoimmune diabetes mellitus (T1D), T1D with insulitis (T1D/INS), and diabetes mellitus type 2 (T2D). We found elevated levels of cCK-18 (P<0.001), I-FABP (P<0.01) and sCD14 (P<0.05) in CLD when compared to healthy controls. However, the levels of cCK-18 (P<0.01) and I-FABP (P<0.01) in CLD-GFD were higher when compared with controls. Interestingly, elevated levels of cCK-18 and I-FABP were found in T2D and T1D (P<0.001), and T1D/INS (P<0.01, P<0.001). Twenty-two out of 43 CLD patients were seropositive for cCK-18, 19/43 for I-FABP and 11/43 for sCD14; 9/30 of T2D patients were positive for cCK-18 and 5/20 of T1D/INS for sCD14, while in controls only 3/41 were positive for cCK-18, 3/41 for I-FABP and 1/41 for sCD14. We documented for the first time seropositivity for sCD14 in CLD and potential usefulness of serum cCK-18 and I-FABP as markers of gut damage in CLD, CLD-GFD, and diabetes.
authors.affiliation: Second Department of Internal Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic, Department of Immunology, Institute of Microbiology, v.v.i., Czech Academy of Sciences, Prague, Czech Republic. sanchez@biomed.cas.cz.
authors.forename: I
authors.initials: I
authors.lastname: Hoffmanová
authors.forename: D
authors.initials: D
authors.lastname: Sánchez
authors.forename: V
authors.initials: V
authors.lastname: Hábová
authors.forename: M
authors.initials: M
authors.lastname: Anděl
authors.forename: L
authors.initials: L
authors.lastname: Tučková
authors.forename: H
authors.initials: H
authors.lastname: Tlaskalová-Hogenová
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D002446:Celiac Disease / Q000097:blood* / Q000453:epidemiology / Q000473:pathology; D015897:Comorbidity; D016207:Cytokines / Q000097:blood*; D018153:Czech Republic / Q000453:epidemiology; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000453:epidemiology / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000453:epidemiology / Q000473:pathology; D020895:Enterocytes / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D008107:Liver Diseases / Q000097:blood* / Q000453:epidemiology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D015203:Reproducibility of Results; D012307:Risk Factors; D012680:Sensitivity and Specificity; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines