title: Direct transesterification of fresh microalgal cells.
abstract: Transesterification of lipids is a vital step during the processes of both biodiesel production and fatty acid analysis. By comparing the yields and fatty acid profiles obtained from microalgal oil and dry microalgal cells, the reliability of method for the transesterification of micro-scale samples was tested. The minimum amount of microalgal cells needed for accurate analysis was found to be approximately 300μg dry cells. This direct transesterification method of fresh cells was applied to eight microalgal species, and the results indicate that the efficiency of the developed method is identical to that of conventional method, except for Spirulina whose lipid content is very low, which means the total lipid content should been considered.
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 16023, China. Electronic address: xuesong@dicp.ac.cn.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D002384:Catalysis; D004951:Esterification; D050356:Lipid Metabolism; D058086:Microalgae / Q000378:metabolism*; D013696:Temperature
chemical_list: D056804:Biofuels
keywords: Direct transesterification; Micro-scale sample; Microalgal cells without dehydration