title: Optimization of Ultrasonic Extraction of Phenolic Compounds from Epimedium brevicornum Maxim Using Response Surface Methodology and Evaluation of Its Antioxidant Activities In Vitro.
abstract: The ultrasound-assisted extraction of phenolic compounds from Epimedium brevicornu Maxim was modeled using response surface methodology. A Central Composite Design (CCD) was employed to optimize three extraction variables, including ethanol concentration (X 1), extraction time (X 2), and ratio of aqueous ethanol to raw material (X 3), for the achievement of high extraction yield of the phenolic compounds. The optimized conditions are X 1 of 50% (v/v), X 2 of 27.5 min, and X 3 of 250 mL/g. Under these conditions, the experimental yield is 4.29 ± 0.033% (n = 3). The antioxidant activity was evaluated using the DPPH assay and ferric-reducing antioxidant power (FRAP). And it indicates that the phenolic compounds from Epimedium brevicornu Maxim possess significant antioxidant activity. HPLC analysis reveals that the main phenolic compound in the extract product was identified as gallic acid, catechin (Cianidanol), p-hydroxybenzoic acid, vanillic acid, caffeic acid, ferulaic acid, rutin, benzoic acid, and quercetin.
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hou
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Tang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Enbo
authors.initials: E
authors.lastname: Cai
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Shuangli
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: He
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Chinese Medicinal Materials, Jilin Agricultural University, No. 2888 Xincheng Street, Changchun, Jilin 130118, China.
authors.forename: Lianxue
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Traditional Chinese Medicine, Jilin Agricultural Science and Technology University, Jilin 132101, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Wang