title: Ensemble velocity of non-processive molecular motors with multiple chemical states.
abstract: We study the ensemble velocity of non-processive motor proteins, described with multiple chemical states. In particular, we discuss the velocity as a function of ATP concentration. Even a simple model which neglects the strain dependence of transition rates, reverse transition rates and nonlinearities in the elasticity can show interesting functional dependencies, which deviate significantly from the frequently assumed Michaelis-Menten form. We discuss how the order of events in the duty cycle can be inferred from the measured dependence. The model also predicts the possibility of velocity reversal at a certain ATP concentration if the duty cycle contains several conformational changes of opposite directionalities.
authors.affiliation: J. Stefan Institute , Jamova 39, 1000 Ljubljana , Slovenia ; Faculty of Mathematics and Physics , University of Ljubljana , Jadranska 19, 1000 Ljubljana , Slovenia.
authors.forename: Andrej
authors.identifier: 0000-0001-8985-6072
authors.initials: A
authors.lastname: Vilfan
keywords: biophysical theory; dynein; kinesin; motility assay; motor proteins; myosin