title: [Comparative study on alkaloids of tissue-culture seedling and wild plant of Dendrobium huoshanense ].
abstract: OBJECTIVE
  To compare the composition and content of alkaloid of Dendrobium huoshanense tissue-culture seedling and wild plant.
  
  
  METHODS
  A comparative evaluation on the quality was carried out by HPLC and TLC methods including the composition and the content of alkaloids.
  
  
  RESULTS
  Remarkable variation existed in the two kinds of Dendrobium huoshanense. For the tissue-culture plant, only two alkaloids were checked out by both HPLC and TLC while four alkaloids were observed in the wild plant. The alkaloid content of tissue-culture seedling and wild plant was(0. 29 ± 0. 11)%o and(0. 43 ± 0. 15) %o,respectively.
  
  
  CONCLUSIONS
  Distinguished difference is observed in both composition and content of alkaloids from the annual shoots of different provenances of Dendrobium huoshanense. It suggested that the quality of tissue-culture seedling of Dendrobium huoshanense might be inconsistent with the wild plant. Furthermore, the established alkaloids-knock-out HPLC method would provide a new research tool on quality control of Chinese medicinal materials which contain unknown alkaloids.
authors.forename: Nai-dong
authors.initials: ND
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D031667:Dendrobium / Q000737:chemistry*; D036226:Seedlings
chemical_list: D000470:Alkaloids