title: Anti-angiogenic activity of Entada africana root.
abstract: Entada africana roots are used in African traditional medicine for various diseases including inflammation. This application may be mediated through anti-angiogenic effects. Thus, in this study the anti-angiogenic activity of E. africana root extracts (n-hexane, chloroform, chloroform/methanol and methanol) was preliminarily evaluated by the quantitative determination of endogenous alkaline phosphatase in zebrafish embryos. A bioactivity-guided fractionation of chloroform/methanol extract yielded apigenin and robinetin as the main constituents from the most active fractions. In addition, a marked reduction on capillary formation was evidenced in chick chorioallantoic membrane after treatment with the active fractions or isolated compounds. Results obtained in this study suggest that the anti-angiogenic effects of E. africana root may account for its use in inflammatory diseases and other related pathological conditions.
authors.affiliation: a Dipartimento di Scienze del Farmaco e Prodotti per la Salute (SCIFAR) , University of Messina , SS. Annunziata, 98168 Messina , Italy.
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Germanò
authors.forename: Giovanna
authors.initials: G
authors.lastname: Certo
authors.forename: Valeria
authors.initials: V
authors.lastname: D'Angelo
authors.forename: Rokia
authors.initials: R
authors.lastname: Sanogo
authors.forename: Nicola
authors.initials: N
authors.lastname: Malafronte
authors.forename: Nunziatina
authors.initials: N
authors.lastname: De Tommasi
authors.forename: Antonio
authors.initials: A
authors.lastname: Rapisarda
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification; D002642:Chick Embryo; D004625:Embryo, Nonmammalian / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification; D016488:Medicine, African Traditional; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D015027:Zebrafish
chemical_list: D020533:Angiogenesis Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D047310:Apigenin; C040014:robinetin
keywords: CAM; Entada africana; anti-angiogenesis; chick chorioallantoic membrane; flavonoids; zebrafish