title: Age-related differential gene and protein expression in postnatal cartilage canal and osteochondral junction chondrocytes.
abstract: Wnt/β-catenin, Indian hedgehog (Ihh)/Parathyroid-related peptide (PTHrP) and retinoid signaling pathways regulate cartilage differentiation, growth, and function during development and play a key role in endochondral ossification. The objective of this study was to elucidate the gene and protein expression of signaling molecules of these regulatory pathways in chondrocytes surrounding cartilage canals and the osteochondral junction during neonatal and pre-adolescent development. This study revealed cell-specific and age-related differences in gene and protein expression of signaling molecules of these regulatory pathways. A trend for higher gene expression of PTHrP along the cartilage canals and Ihh along the osteochondral junction suggests the presence of paracrine feedback in articular-epiphyseal cartilage. Differential expression of canonical (β-catenin, Wnt-4, Lrp4, Lrp6) and noncanonical Wnt signaling (Wnt-5b, Wnt-11) and their inhibitors (Dkk1, Axin1, sFRP3, sFRP5, Wif-1) surrounding the cartilage canals and osteochondral junction provides evidence of the complex interactions occurring during endochondral ossification.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Katja
authors.initials: K
authors.lastname: Duesterdieck-Zellmer
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA. Electronic address: stacy.semevolos@oregonstate.edu.
authors.forename: Stacy
authors.initials: S
authors.lastname: Semevolos
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Kinsley
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
authors.forename: Tara
authors.initials: T
authors.lastname: Riddick
mesh_terms: D000375:Aging / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation; D019902:Chondrocytes / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D006132:Growth Plate / Q000378:metabolism*; D006736:Horses / Q000235:genetics* / Q000378:metabolism; D010012:Osteogenesis; D060449:Wnt Signaling Pathway
keywords: Beta-catenin; Cartilage development; Indian hedgehog; PTHrP; Wnt signaling