title: Susceptibility of GT1-7 cells to mouse-passaged field scrapie isolates with a long incubation.
abstract: A typical feature of scrapie in sheep and goats is the accumulation of disease-associated prion protein. Scrapie consists of many strains with different biological properties. Nine natural sheep scrapie cases were transmitted to wild-type mice and mouse-passaged isolates were classified into 2 types based on incubation time: short and long. These 2 types displayed a distinct difference in their pathology. We attempted to transmit these mouse-passaged isolates to 2 murine cell lines (GT1-7 and L929) to compare their properties. All of the isolates were transmitted to L929 cells. However, only mouse-passaged field isolates with a long incubation time were transmitted to GT1-7 cells. This specific susceptibility of GT1-7 cells was also confirmed with a primary-passaged isolate that was not completely adapted to the new host species. Characterization of the mechanisms of the specific susceptibility of GT1-7 cells to isolates with a long incubation time may lead to a greater understanding of the differences among prion strains.
authors.affiliation: a Influenza and Prion Disease Research Center ; National Institute of Animal Health; NARO ; Tsukuba , Ibaraki , Japan.
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Miyazawa
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Okada
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Iwamaru
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masujin
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice / Q000502:physiology; D018620:PrPSc Proteins / Q000032:analysis* / Q000378:metabolism; D011487:Protein Conformation; D055550:Protein Stability; D012608:Scrapie / Q000378:metabolism / Q000473:pathology / Q000635:transmission*; D012756:Sheep / Q000502:physiology
chemical_list: D018620:PrPSc Proteins
keywords: cell susceptibility, prion, scrapie, strains