title: MiR-153 inhibits migration and invasion of human non-small-cell lung cancer by targeting ADAM19.
abstract: MiR-153 was reported to be dysregulated in some human cancers. However, the function and mechanism of miR-153 in lung cancer cells remains unknown. In this study, we investigated the role of miR-153 in human non-small-cell lung cancer (NSCLC). Using qRT-PCR, we demonstrated that miR-153 was significantly decreased in clinical NSCLC tissues and cell lines, and downregulation of miR-153 was significantly correlated with lymph node status. We further found that ectopic expression of miR-153 significantly inhibited the proliferation and migration and invasion of NSCLC cells in vitro, suggesting that miR-153 may be a novel tumor suppressor in NSCLC. Further integrated analysis revealed that ADAM19 is as a direct and functional target of miR-153. Luciferase reporter assay demonstrated that miR-153 directly targeted 3'UTR of ADAM19, and correlation analysis revealed an inverse correlation between miR-153 and ADAM19 mRNA levels in clinical NSCLC tissues. Knockdown of ADAM19 inhibited migration and invasion of NSCLC cells which was similar with effects of overexpression of miR-153, while overexpression of ADAM19 attenuated the function of miR-153 in NSCLC cells. Taken together, our results highlight the significance of miR-153 and ADAM19 in the development and progression of NSCLC.
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Nianxi
authors.initials: N
authors.lastname: Shan
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Liangfang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China. Electronic address: duancjxy@163.com.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Duan
mesh_terms: D020413:3' Untranslated Regions; D051722:ADAM Proteins / Q000378:metabolism*; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness
chemical_list: D020413:3' Untranslated Regions; C556500:MIRN153 microRNA, human; D035683:MicroRNAs; D051722:ADAM Proteins; C411902:ADAM19 protein, human
keywords: ADAM19; Cell invasion; Cell migration; Human non-small-cell lung cancer; miR-153