title: Hydrolysed fumonisin B1 and N-(deoxy-D-fructos-1-yl)-fumonisin B1: stability and catabolic fate under simulated human gastrointestinal conditions.
abstract: Food processing may induce thermal degradation of fumonisins in corn via Maillard-type reactions, or alkaline hydrolysis via loss of the two tricarballylic acid moieties. In the former case, N-(1-deoxy-D-fructos-1-yl)-fumonisin B(1) (NDF) can be formed, while the latter derivative is called hydrolysed fumonisin B(1) (HFB(1)). The aim of this study was to deepen the knowledge about the gastrointestinal stability of HFB(1) and NDF in humans. Due to the lack of standard, NDF was chemically synthesised and cleaned up in high purity to be used for further experiments. While NDF is already partially cleaved (about 41%) during simulated digestion, it remained rather stable towards human colon microflora. In contrast to this, HFB(1) is partially metabolised by the colon microflora to unknown compounds after 24 h of fermentation, as seen by a loss of about 22%. Concluding, the cleavage of NDF during digestion as well as the likely metabolisation of HFB(1) emphasise the need for animal trials to ascertain their toxicity in vivo.
authors.affiliation: LS9 Bioactives&Health, Interlaboratory Group, Department of Food Science, University of Parma , Parma , Italy and.
authors.forename: Martina
authors.initials: M
authors.lastname: Cirlini
authors.forename: Irene
authors.initials: I
authors.lastname: Hahn
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Varga
authors.forename: Margherita
authors.initials: M
authors.lastname: Dall'Asta
authors.forename: Claudia
authors.initials: C
authors.lastname: Falavigna
authors.forename: Luca
authors.initials: L
authors.lastname: Calani
authors.forename: Franz
authors.initials: F
authors.lastname: Berthiller
authors.forename: Daniele
authors.initials: D
authors.lastname: Del Rio
authors.forename: Chiara
authors.initials: C
authors.lastname: Dall'Asta
mesh_terms: D002853:Chromatography, Liquid; D005285:Fermentation; D005506:Food Contamination; D005511:Food Handling; D005516:Food Microbiology; D037341:Fumonisins / Q000737:chemistry*; D041981:Gastrointestinal Tract / Q000187:drug effects* / Q000382:microbiology; D006801:Humans; D006868:Hydrolysis; D008954:Models, Biological; D053719:Tandem Mass Spectrometry; D014233:Tricarboxylic Acids; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology
chemical_list: D037341:Fumonisins; D014233:Tricarboxylic Acids; C056933:fumonisin B1; C028021:tricarballylic acid
keywords: Bioavailability; food processing; fumonisins; gut microflora; mycotoxins