title: Digital pathology with Fourier ptychography.
abstract: Fourier ptychographic microscopy (FPM) is a recently introduced method of acquiring high-resolution, wide field of view (FOV) giga-pixel histology images. The FPM procedure first acquires a sequence of low-resolution images of a sample under variable-angle illumination. It then combines these images using a novel phase retrieval algorithm to improve the employed microscope's resolution beyond its conventional limit. Here, we first describe how FPM's resolution improvement can enhance wide FOV histology imaging. Second, we show that FPM also records a thin sample's optical phase, which can help pathologists digitally extract as much information as possible from a given histology slide.
authors.affiliation: Department of Electrical Engineering and Bioengineering, California Institute of Technology, Pasadena, CA 91125, United States. Electronic address: roarke@caltech.edu.
authors.forename: Roarke
authors.initials: R
authors.lastname: Horstmeyer
authors.affiliation: Department of Electrical Engineering and Bioengineering, California Institute of Technology, Pasadena, CA 91125, United States.
authors.forename: Xiaoze
authors.initials: X
authors.lastname: Ou
authors.affiliation: Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06268, United States.
authors.forename: Guoan
authors.initials: G
authors.lastname: Zheng
authors.affiliation: ePetri, Inc., Pasadena, CA 91106, United States.
authors.forename: Phil
authors.initials: P
authors.lastname: Willems
authors.affiliation: Department of Electrical Engineering and Bioengineering, California Institute of Technology, Pasadena, CA 91125, United States.
authors.forename: Changhuei
authors.initials: C
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005583:Fourier Analysis; D006652:Histological Techniques / Q000295:instrumentation / Q000379:methods*; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008029:Lighting / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
keywords: Computational imaging; Digital pathology; Microscopy; Structured illumination; Super-resolution