title: Phosphoproteomic analysis of the resistant and susceptible genotypes of maize infected with sugarcane mosaic virus.
abstract: Protein phosphorylation plays a pivotal role in the regulation of many cellular events. No information is yet available, however, on protein phosphorylation in plants in response to virus infection. In this study, we characterized phosphoproteomes of resistant and susceptible genotypes of maize (Zea mays L.) in response to Sugarcane mosaic virus (SCMV) infection. Based on isotope tags for relative and absolute quantification technology, TiO2 enrichment method and LC-MS/MS analysis, we identified 65 and 59 phosphoproteins respectively, whose phosphorylation level regulated significantly in susceptible and resistant plants. Some identified phosphoproteins were shared by both genotypes, suggesting a partial overlapping of the responsive pathways to virus infection. While several phosphoproteins are well-known pathogen response phosphoproteins, virus infection differentially regulates most other phosphoproteins, which has not been reported in literature. Changes in protein phosphorylation status indicated that response to SCMV infection encompass a reformatting of major cellular processes. Our data provide new valuable insights into plant-virus interactions.
authors.affiliation: Henan Agricultural University and Synergetic Innovation Center of Henan Grain Crops, Zhengzhou, China.
authors.forename: Liuji
authors.initials: L
authors.lastname: Wu
authors.forename: Shunxi
authors.initials: S
authors.lastname: Wang
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wu
authors.forename: Zanping
authors.initials: Z
authors.lastname: Han
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Liancheng
authors.initials: L
authors.lastname: Wu
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hu
mesh_terms: D005838:Genotype; D054884:Host-Pathogen Interactions; D009029:Mosaic Viruses / Q000502:physiology*; D010750:Phosphoproteins / Q000378:metabolism*; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D003313:Zea mays / Q000378:metabolism / Q000821:virology
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome