title: Induction of caspase cascade pathway by kaempferol-3-O-rhamnoside in LNCaP prostate cancer cell lines.
abstract: Prostate cancer has become a leading cause of mortality in humans. Previous studies have shown the potential anticancer properties of kaempferol-3-O-rhamnoside in breast cancer cell lines. In the present study, the anticancer potential of kaempferol-3-O-rhamnoside was investigated in LNCaP human prostate cancer cell lines. The inhibition of cell proliferation was investigated using MTT assays, whereas its ability to induce the caspase-cascade pathway was investigated by western blotting. The results showed that kaempferol-3-O-rhamnoside inhibits the proliferation of LNCaP cells in a dose-dependent manner by upregulating the expression of caspase-8, caspase-9, caspase-3 and poly (ADP-ribose) polymerase proteins. Although further studies are required, the results of the present study indicate the potential application of kaempferol-3-O-rhamnoside in cancer treatment.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Eli
authors.initials: E
authors.lastname: Halimah
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Ajeng
authors.initials: A
authors.lastname: Diantini
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Dika P
authors.initials: DP
authors.lastname: Destiani
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Ivan S
authors.initials: IS
authors.lastname: Pradipta
authors.affiliation: Department of Clinical Pharmacology, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, West Java 45363, Indonesia, Japan.
authors.forename: Herri S
authors.initials: HS
authors.lastname: Sastramihardja
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Keri
authors.initials: K
authors.lastname: Lestari
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan.
authors.forename: Anas
authors.initials: A
authors.lastname: Subarnas
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Japan ; Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Rizky
authors.initials: R
authors.lastname: Abdulah
authors.affiliation: Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Koyama
keywords: LNCaP; apoptosis; cancer; caspase; kaempferol-3-O-rhamnoside