title: Removal of Carbapenem-Resistant Enterobacteriaceae (CRE) from blood by heparin-functional hemoperfusion media.
abstract: Bloodstream infections due to Carbapenem-Resistant Enterobacteriaceae (CRE) are becoming more frequent and are associated with a high mortality. At present, combination antimicrobial therapy yields the best outcomes, but treatment options are limited. Many bacteria utilize heparan sulfate to bind to human cells. We studied the ability of a biomimetic device composed of polyethylene beads with endpoint-attached heparin to bind both sensitive and (CRE) E. coli and Klebsiella pneumoniae from spiked blood samples. Greater than 90% of susceptible, E. coli, CRE E. coli and CRE Klebsiella were removed by the beads. Future studies in human bacteremia with this technology are planned.
authors.affiliation: ExThera Medical Corporation, 813 Heinz Avenue, Berkeley, CA 94710, United States of America.
authors.forename: Keith
authors.initials: K
authors.lastname: McCrea
authors.affiliation: ExThera Medical Corporation, 813 Heinz Avenue, Berkeley, CA 94710, United States of America.
authors.forename: Robert
authors.initials: R
authors.lastname: Ward
authors.affiliation: Infectious Disease, Beverly Hospital/Lahey Health System, 85 Herrick Street, Beverly, MA 01915, United States of America.
authors.forename: Steven P
authors.initials: SP
authors.lastname: LaRosa
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000097:blood / Q000382:microbiology*; D001422:Bacterial Adhesion; D015780:Carbapenems / Q000494:pharmacology; D004926:Escherichia coli / Q000302:isolation & purification* / Q000502:physiology; D006464:Hemoperfusion / Q000379:methods*; D006493:Heparin / Q000737:chemistry*; D006736:Horses; D006801:Humans; D007711:Klebsiella pneumoniae / Q000302:isolation & purification* / Q000502:physiology; D018440:beta-Lactam Resistance
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D006493:Heparin