title: Glutamic acid decarboxylase levels in the cochlear nucleus of rats with acoustic trauma-induced chronic tinnitus.
abstract: Tinnitus is the perception of phantom sounds, a phenomenon believed to be due to abnormal neuronal activity in auditory regions of the CNS such as the brainstem cochlear nucleus (CN). One possible mechanism for the abnormal neuronal activity in the CN, supported by recent animal studies, is a decrease in GABAergic inhibition. One possible explanation for this is a decrease in the enzyme responsible for the synthesis of GABA, glutamic acid decarboxylase (GAD). In this study, we used immunohistochemistry to analyse the levels of GAD in the dorsal and ventral CN of rats that had been exposed to noise trauma and had been confirmed to exhibit psychophysical evidence of tinnitus (at 17.5 weeks post-exposure) using a conditioned behavioural task. At 22 weeks following noise trauma or sham treatment, the levels of GAD in the dorsal and ventral CN were not significantly different. This result suggests that acoustic trauma that can cause chronic tinnitus is not associated with changes in GAD in the CN at 22 weeks post-exposure.
authors.affiliation: Department of Pharmacology and Toxicology, School of Medical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand. Electronic address: yiwen.zheng@otago.ac.nz.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Pharmacology and Toxicology, School of Medical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dixon
authors.affiliation: Department of Pharmacology and Toxicology, School of Medical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
authors.forename: Kate
authors.initials: K
authors.lastname: McPherson
authors.affiliation: Department of Pharmacology and Toxicology, School of Medical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
authors.forename: Kate
authors.initials: K
authors.lastname: MacPherson
authors.affiliation: Department of Pharmacology and Toxicology, School of Medical Sciences and the Brain Health Research Centre, University of Otago, Dunedin, New Zealand.
authors.forename: Paul F
authors.initials: PF
authors.lastname: Smith
mesh_terms: D000818:Animals; D001418:Baclofen / Q000494:pharmacology; D002908:Chronic Disease; D017626:Cochlear Nucleus / Q000187:drug effects / Q000201:enzymology*; D058786:GABA-B Receptor Agonists / Q000494:pharmacology; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D006317:Hearing Loss, Noise-Induced / Q000150:complications*; D008297:Male; D017208:Rats, Wistar; D014012:Tinnitus / Q000201:enzymology* / Q000209:etiology
chemical_list: D058786:GABA-B Receptor Agonists; D005968:Glutamate Decarboxylase; D001418:Baclofen
keywords: Cochlear nucleus; GABA; Glutamic acid decarboxylase; Rat; Tinnitus