title: A novel GMO biosensor for rapid ultrasensitive and simultaneous detection of multiple DNA components in GMO products.
abstract: Since the introduction of genetically modified organisms (GMOs), there has been on-going and continuous concern and debates on the commercialization of products derived from GMOs. There is an urgent need for development of highly efficient analytical methods for rapid and high throughput screening of GMOs components, as required for appropriate labeling of GMO-derived foods, as well as for on-site inspection and import/export quarantine. In this study, we describe, for the first time, a multi-labeling based electrochemical biosensor for simultaneous detection of multiple DNA components of GMO products on the same sensing interface. Two-round signal amplification was applied by using both an exonuclease enzyme catalytic reaction and gold nanoparticle-based bio-barcode related strategies, respectively. Simultaneous multiple detections of different DNA components of GMOs were successfully achieved with satisfied sensitivity using this electrochemical biosensor. Furthermore, the robustness and effectiveness of the proposed approach was successfully demonstrated by application to various GMO products, including locally obtained and confirmed commercial GMO seeds and transgenetic plants. The proposed electrochemical biosensor demonstrated unique merits that promise to gain more interest in its use for rapid and on-site simultaneous multiple screening of different components of GMO products.
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: National Engineering Laboratory of Food Storage and Transportation, Nanjing University of Finances and Economics, Nanjing 210023, PR China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China; School of Chemistry, Monash University, Clayton, Vic 3800, Australia. Electronic address: sam.adeloju@monash.edu.
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Adeloju
authors.affiliation: School of Biotechnology & Food Engineering, Key Laboratory of Functional Materials and Devices of Anhui Province, Hefei University of Technology, Hefei 230009, PR China. Electronic address: chenweishnu@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D015342:DNA Probes; D004274:DNA, Recombinant / Q000032:analysis* / Q000235:genetics*; D005524:Food Technology; D030841:Food, Genetically Modified; D006046:Gold; D053768:Metal Nanoparticles; D021141:Nucleic Acid Amplification Techniques; D030781:Organisms, Genetically Modified / Q000235:genetics*; D010084:Oxidation-Reduction
chemical_list: D015342:DNA Probes; D004274:DNA, Recombinant; D006046:Gold
keywords: Electrochemical biosensor; Food safety; Genetically modified organism; Rapid detection; Simultaneous and multiple detection