title: Foot-and-mouth disease vaccination induces cross-reactive IFN-γ responses in cattle that are dependent on the integrity of the 140S particles.
abstract: Interferon-γ (IFN-γ) recall responses against foot-and-mouth disease virus (FMDV) in FMD vaccinated cattle are utilized to study T-lymphocyte immunity against this virus. Here, a recall IFN-γ assay based on a commercial ELISA was set up using 308 samples from naïve and vaccinated cattle. The assay was used to study cross-reactive responses between different FMDV vaccine strains. Blood samples from cattle immunized with monovalent vaccines containing A24/Cruzeiro/Brazil/55, A/Argentina/2001 or O1/Campos/Brazil/58 strains were tested using purified-inactivated FMDV from homologous and heterologous strains. A24/Cruzeiro was the most efficient IFN-γ inducer in all vaccinated animals, both when included in the vaccine or as stimulating antigen. We demonstrate that this was mainly due to the structural stability of the whole viral particle. These results show that IFN-γ production relies on the presence of 140S particles that can maintain their integrity along the incubation process in vitro, and throughout the vaccine's shelf-life, when used in vivo.
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. Electronic address: bucafusco.danilo@inta.gob.ar.
authors.forename: Danilo
authors.initials: D
authors.lastname: Bucafusco
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina. Electronic address: digiacomo.sebastian@inta.gob.ar.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Di Giacomo
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. Electronic address: pega.juan@inta.gob.ar.
authors.forename: Juan
authors.initials: J
authors.lastname: Pega
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina. Electronic address: schammas.juan@inta.gob.ar.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Schammas
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina. Electronic address: cardoso.nancy@inta.gob.ar.
authors.forename: Nancy
authors.initials: N
authors.lastname: Cardoso
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. Electronic address: capozzo.alejandra@inta.gob.ar.
authors.forename: Alejandra Victoria
authors.initials: AV
authors.lastname: Capozzo
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto Nacional de Tecnología Agropecuaria (INTA), N Repetto y De Los Reseros s/n, Hurlingham (1686), Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina. Electronic address: perez.mariano@inta.gob.ar.
authors.forename: Mariano
authors.initials: M
authors.lastname: Perez-Filgueira
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology*; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*
chemical_list: D014765:Viral Vaccines; D007371:Interferon-gamma
keywords: Capsid integrity; Cattle; FMD vaccines; FMDV; IFN-γ