title: Application of a new oxidation-reduction potential assessment method in strenuous exercise-induced oxidative stress.
abstract: OBJECTIVE
  The aim of the study was to test a novel method for assessing oxidative stress, the RedoxSYS™ diagnostic system, a holistic, fast, minimally invasive, and requiring small sample volume method, that measures two parameters, the static (sORP) and the capacity (cORP) oxidation-reduction potential.
  
  
  METHODS
  The redox status of 14 athletes participating in a mountain marathon race was assessed. Redox status in blood obtained 1 day before the race and immediately after the race was assessed using the RedoxSYS diagnostic system as well as conventional oxidative stress markers such as glutathione levels (GSH), catalase activity (CAT), thiobarbituric acid reactive substances (TBARS), protein carbonyls (CARB), and total antioxidant activity.
  
  
  RESULTS
  The results showed that after the race, the sORP was increased significantly by 7% indicating oxidative stress induction, while cORP was decreased by 14.6% but not significantly. Moreover, the conventional oxidative stress markers GSH and CAT were decreased significantly by 13.1 and 23.4%, respectively, while TBARS and CARB were increased significantly by 26.1 and 15.6%, respectively, after the race indicating oxidative stress induction.
  
  
  CONCLUSIONS
  The present study demonstrated for the first time that the RedoxSYS diagnostic system can be used for evaluating the exercise-induced oxidative stress in athletes.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stagos
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Goutzourelas
authors.forename: David
authors.initials: D
authors.lastname: Bar-Or
authors.forename: Amalia-Maria
authors.initials: AM
authors.lastname: Ntontou
authors.forename: Evangelia
authors.initials: E
authors.lastname: Bella
authors.forename: Aphrodite Tousia
authors.initials: AT
authors.lastname: Becker
authors.forename: Argyro
authors.initials: A
authors.lastname: Statiri
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kafantaris
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Kouretas
mesh_terms: D000328:Adult; D000531:Altitude; D000975:Antioxidants / Q000032:analysis; D056352:Athletes; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis; D002374:Catalase / Q000097:blood; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D007249:Inflammation / Q000097:blood; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D005082:Physical Exertion / Q000502:physiology*; D050050:Protein Carbonylation; D012420:Running / Q000502:physiology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D017392:Thiobarbituric Acid Reactive Substances; D002097:C-Reactive Protein; D002374:Catalase; D005978:Glutathione
keywords: Exercise; Oxidation-reduction potential; Oxidative stress marker; RedoxSYS diagnostic system