title: IL-6, IL-10, c-Jun and STAT3 expression in B-CLL.
abstract: Chronic lymphocytic leukemia is characterized by the accumulation of functionally abnormal, monoclonal B lymphocytes in the peripheral blood, bone marrow, lymph nodes and spleen, resulting in a reduction count of normal immunocompetent cells and their impaired immune function. The defect in transmission of signals from various types of extracellular receptors, leading to aberrant cytokines and transcription factors gene expression, may underlie the basis of immune failure in B-CLL. The aim of the study was to assess of IL-6, IL-10, c-Jun, and STAT3 expression. In response to antigenic stimulation IL-6, IL-10, c-Jun, and STAT3 proteins induce mutual activity. The subject of the study was subpopulations of leukemic lymphocytes (CD5+ CD19+) and CD19+ B cells from healthy donors (control group). Our results provide evidence that the regulation of IL-6, IL-10, c-Jun, and STAT3 gene expression in CLL B cells is clearly different from normal B lymphocytes. In B-CLL STAT3 expression in unstimulated lymphocytes is significantly higher (p<0.0001) compared with normal subpopulation of B cell. In contrast, IL-6, IL-10, and c-Jun mRNA expressions are statistically lower in B-CLL in comparison with the control group, in all cases (p<0.0001). When analyzing the relationship between c-Jun expression and B-CLL stage according Rai we revealed decreasing c-Jun expression, both at the mRNA and protein levels, along with advancing stage of disease.
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland. Electronic address: hagenetyka@wp.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Antosz
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wojciechowska
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Sajewicz
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Choroszyńska
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Marzec-Kotarska
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Osiak
authors.affiliation: Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pająk
authors.affiliation: Department of Haematooncology and Bone Marrow Transplantation, Medical University of Lublin, Lublin, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Tomczak
authors.affiliation: Department of Cardiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Jargiełło-Baszak
authors.affiliation: Department of Cardiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Baszak
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis / Q000235:genetics*; D015850:Interleukin-6 / Q000032:analysis / Q000235:genetics*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000032:analysis / Q000235:genetics*
chemical_list: D015850:Interleukin-6; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D016753:Interleukin-10
keywords: B-CLL; IL-10; IL-6; STAT3; c-Jun