title: Immunoproteomic characterisation of Mycoplasma mycoides subspecies capri by mass spectrometry analysis of two-dimensional electrophoresis spots and western blot.
abstract: OBJECTIVE
  Mycoplasma mycoides subspecies capri is one of the causative agents of contagious agalactia in goats. The disease is characterised by mastitis, pneumonia, arthritis, keratitis and in acute cases septicaemia. No vaccine is currently available that has been demonstrated to prevent disease.
  
  
  METHODS
  This study used two-dimensional electrophoresis to separate proteins from whole-cell preparations and tandem mass spectrometry to identify them.
  
  
  RESULTS
  In total, 145 spots were successfully identified corresponding to 74 protein identities. Twenty of these proteins were found to be immunogenic by western blot analysis using a pooled serum sample from experimentally infected goats.
  
  
  CONCLUSIONS
  Six proteins were found to have a less than 95% amino acid similarity to a closely related Mycoplasma species showing that they warrant further evaluation in development of diagnostic tests. These proteins were a dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex, phosphoglycerate kinase, pyrimidine-nucleoside phosphorylase, 30S ribosomal protein S6, ribulose-phosphate 3-epimerase and D-lactate dehydrogenase.
authors.affiliation: School of Life Sciences, Faculty of Science, Engineering and Computing, Kingston University, London, KT1 2EE, UK.
authors.forename: Colin P
authors.initials: CP
authors.lastname: Churchward
authors.forename: Rubén S
authors.initials: RS
authors.lastname: Rosales
authors.forename: Adriana
authors.initials: A
authors.lastname: Gielbert
authors.forename: Mercedes
authors.initials: M
authors.lastname: Domínguez
authors.forename: Robin A J
authors.initials: RA
authors.lastname: Nicholas
authors.forename: Roger D
authors.initials: RD
authors.lastname: Ayling
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001426:Bacterial Proteins / Q000097:blood* / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006041:Goats; D013058:Mass Spectrometry / Q000379:methods; D009176:Mycoplasma mycoides / Q000145:classification / Q000378:metabolism*; D011002:Pleuropneumonia, Contagious / Q000097:blood* / Q000382:microbiology; D020543:Proteome; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D020543:Proteome
keywords: Immunoproteomics; biological characterisation of novel molecules