title: Inorganic-organic thin implant coatings deposited by lasers.
abstract: The lifetime of bone implants inside the human body is directly related to their osseointegration. Ideally, future materials should be inspired by human tissues and provide the material structure-function relationship from which synthetic advanced biomimetic materials capable of replacing, repairing, or regenerating human tissues can be produced. This work describes the development of biomimetic thin coatings on titanium implants to improve implant osseointegration. The assembly of an inorganic-organic biomimetic structure by UV laser pulses is reported. The structure consists of a hydroxyapatite (HA) film grown onto a titanium substrate by pulsed-laser deposition (PLD) and activated by a top fibronectin (FN) coating deposited by matrix-assisted pulsed laser evaporation (MAPLE). A pulsed KrF* laser source (λ = 248 nm, τ = 25 ns) was employed at fluences of 7 and 0.7J/cm(2) for HA and FN transfer, respectively. Films approximately 1500 and 450 nm thick were obtained for HA and FN, respectively. A new cryogenic temperature-programmed desorption mass spectrometry analysis method was employed to accurately measure the quantity of immobilized protein. We determined that less than 7 μg FN per cm(2) HA surface is adequate to improve adhesion, spreading, and differentiation of osteoprogenitor cells. We believe that the proposed fabrication method opens the door to combining and immobilizing two or more inorganic and organic materials on a solid substrate in a well-defined manner. The flexibility of this method enables the synthesis of new hybrid materials by simply tailoring the irradiation conditions according to the thermo-physical properties of the starting materials.
authors.affiliation: Lasers Department, National Institute for Lasers, Plasma and Radiation Physics , 409 Atomistilor Street, Magurele, Ilfov, RO-77125, Romania.
authors.forename: Felix
authors.initials: F
authors.lastname: Sima
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Davidson
authors.forename: Joseph
authors.initials: J
authors.lastname: Dentzer
authors.forename: Roger
authors.initials: R
authors.lastname: Gadiou
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
authors.forename: Olivier
authors.initials: O
authors.lastname: Gallet
authors.forename: Ion N
authors.initials: IN
authors.lastname: Mihailescu
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
mesh_terms: D032701:Biomimetics; D001842:Bone and Bones / Q000166:cytology; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009930:Organic Chemicals / Q000737:chemistry; D016348:Osseointegration; D010006:Osteoblasts / Q000166:cytology; D019736:Prostheses and Implants; D011474:Prosthesis Design; D011485:Protein Binding; D012038:Regeneration; D013053:Spectrophotometry; D013234:Stem Cells / Q000166:cytology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D009930:Organic Chemicals; D017886:Durapatite; D014025:Titanium
keywords: cellular studies; hybrid materials; hydroxyapatite-fibronectin thin films; laser techniques; protein quantification method