title: On the method development of immobilized polysaccharide chiral stationary phases in supercritical fluid chromatography using an extended range of modifiers.
abstract: Polysaccharide-derived selectors are often used in the separation of enantiomers by supercritical fluid chromatography (SFC). Their recognition patterns are normally investigated with alcohols and acetonitrile as modifiers. The present paper describes the results of a research program designed by Pfizer and Chiral Technologies Inc. to explore the potential of other solvents (i.e. ethyl acetate, tetrahydrofuran, dichloromethane) in SFC by using a series of polysaccharide-derived supports with broad solvent versatility (CHIRALPAK IA, IB, IC, ID, IE and IF). The contribution of such extended solvent range to the overall success rate, as well as to overcome racemization, solubility and stability issues was confirmed by using standard non-proprietary samples and research molecules. Elution patterns with such lower polarity solvents, compared to alcohols, and the role of the different additives were also investigated.
authors.affiliation: Chiral Technologies Inc., 800 North Five Pointe Road, West Chester, PA 19380, USA.
authors.forename: James
authors.initials: J
authors.lastname: Lee
authors.affiliation: Chiral Technologies Inc., 800 North Five Pointe Road, West Chester, PA 19380, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Lee
authors.affiliation: Chiral Technologies Inc., 800 North Five Pointe Road, West Chester, PA 19380, USA.
authors.forename: William L
authors.initials: WL
authors.lastname: Watts
authors.affiliation: Chiral Technologies Inc., 800 North Five Pointe Road, West Chester, PA 19380, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Barendt
authors.affiliation: Pfizer Global R&D, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA.
authors.forename: Tony Q
authors.initials: TQ
authors.lastname: Yan
authors.affiliation: Pfizer Global R&D, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Pfizer Global R&D, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA.
authors.forename: Frank
authors.initials: F
authors.lastname: Riley
authors.affiliation: Pfizer Global R&D, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Hardink
authors.affiliation: Pfizer Global R&D, Pfizer Inc., Eastern Point Road, Groton, CT 06340, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Bradow
authors.affiliation: Chiral Technologies Europe, Bd. Gonthier d'Andernach, 67404 Illkirch Cedex, France. Electronic address: pfranco@chiral.fr.
authors.forename: Pilar
authors.initials: P
authors.lastname: Franco
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D011134:Polysaccharides; D012997:Solvents
keywords: Additive effect; Chemically immobilized polysaccharide-based chiral stationary phases; Enantiomer resolution by supercritical fluid chromatography; Mobile phase effect; SFC