title: A two-photon fluorescent probe with a large turn-on signal for imaging hydrogen sulfide in living tissues.
abstract: A two-photon fluorescence turn-on H2S probe GCTPOC-H2S based on a two-photon platform with a large cross-section, GCTPOC, and a sensitive H2S recognition site, dinitrophenyl ether was constructed. The probe GCTPOC-H2S exhibits desirable properties such as high sensitivity, high selectivity, functioning well at physiological pH and low cytotoxicity. In particular, the probe shows a 120-fold enhancement in the presence of Na2S (500 μM), which is larger than the reported two-photon fluorescent H2S probes. The large fluorescence enhancement of the two-photon probe GCTPOC-H2S renders it attractive for imaging H2S in living tissues with deep tissue penetration. Significantly, we have demonstrated that the probe GCTPOC-H2S is suitable for fluorescence imaging of H2S in living tissues with deep penetration by using two-photon microscopy. The further application of the two-photon probe for the investigation of biological functions and pathological roles of H2S in living systems is under progress.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.
authors.forename: Kaibo
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, University of Jinan, Jinan, Shandong 250022, PR China; State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China. Electronic address: weiyinglin2013@163.com.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008027:Light; D008099:Liver / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D017785:Photons; D011789:Quantum Theory
chemical_list: D005456:Fluorescent Dyes; D006862:Hydrogen Sulfide
keywords: Bioimaging; H(2)S; Tissues; Two-photon fluorescent probe