title: Efficient production of isoflavonoids by Astragalus membranaceus hairy root cultures and evaluation of antioxidant activities of extracts.
abstract: In this study, Astragalus membranaceus hairy root cultures (AMHRCs) were established as an attractive alternative source for the efficient production of isoflavonoids (IF). A. membranaceus hairy root line II was screened as the most efficient line and was confirmed by PCR amplification of rolB, rolC and aux1 genes. Culture parameters of AMHRCs were systematically optimized, and five main IF constituents were quali-quantitatively determined by LC-MS/MS. Under optimal conditions, the total IF accumulation of 34 day old AMHRCs was 234.77 μg/g dry weight (DW). This yield was significantly higher compared to that of 3 year old field grown roots (187.38 μg/g DW). Additionally, in vitro antioxidant assays demonstrated that AMHRC extracts exhibited antioxidant activities with lower IC50 values (1.40 and 1.73 mg/mL) as compared to those of field grown roots (1.96 and 2.17 mg/mL). Overall, AMHRCs may offer a promising and continuous product platform for naturally derived, high quality and valuable nutraceuticals.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding and §Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University , Harbin, Heilongjiang 150040, People's Republic of China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Jiao
authors.forename: Qing-Yan
authors.initials: QY
authors.lastname: Gai
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Xiao
authors.initials: X
authors.lastname: Peng
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Tan
authors.forename: Thomas
authors.initials: T
authors.lastname: Efferth
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D027885:Astragalus propinquus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000378:metabolism; D007529:Isoflavones / Q000032:analysis* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D007529:Isoflavones
keywords: Astragalus membranaceus; LC−MS/MS; antioxidant activities; hairy roots; isoflavonoids; response surface methodology