title: Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis.
abstract: The cell surface is the cellular compartment responsible for communication with the environment. The interior of mammalian cells undergoes dramatic reorganization when cells enter mitosis. These changes are triggered by activation of the CDK1 kinase and have been studied extensively. In contrast, very little is known of the cell surface changes during cell division. We undertook a quantitative proteomic comparison of cell surface-exposed proteins in human cancer cells that were tightly synchronized in mitosis or interphase. Six hundred and twenty-eight surface and surface-associated proteins in HeLa cells were identified; of these, 27 were significantly enriched at the cell surface in mitosis and 37 in interphase. Using imaging techniques, we confirmed the mitosis-selective cell surface localization of protocadherin PCDH7, a member of a family with anti-adhesive roles in embryos. We show that PCDH7 is required for development of full mitotic rounding pressure at the onset of mitosis. Our analysis provided basic information on how cell cycle progression affects the cell surface. It also provides potential pharmacodynamic biomarkers for anti-mitotic cancer chemotherapy.
authors.affiliation: Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey Proteomics Center at Children's Hospital Boston, Boston, MA, USA Department of Systems Biology, Harvard Medical School, Boston, MA, USA nozlu@ku.edu.tr.
authors.forename: Nurhan
authors.initials: N
authors.lastname: Özlü
authors.affiliation: Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey.
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: Qureshi
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Toyoda
authors.affiliation: Research Group Bioinformatics (NG 4), Robert Koch-Institute, Berlin, Germany.
authors.forename: Bernhard Y
authors.initials: BY
authors.lastname: Renard
authors.affiliation: Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey.
authors.forename: Gürkan
authors.initials: G
authors.lastname: Mollaoglu
authors.affiliation: Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey.
authors.forename: Nazlı E
authors.initials: NE
authors.lastname: Özkan
authors.affiliation: Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey.
authors.forename: Selda
authors.initials: S
authors.lastname: Bulbul
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Poser
authors.affiliation: Proteomics Center at Children's Hospital Boston, Boston, MA, USA.
authors.forename: Wiebke
authors.initials: W
authors.lastname: Timm
authors.affiliation: Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Hyman
authors.affiliation: Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Mitchison
authors.affiliation: Proteomics Center at Children's Hospital Boston, Boston, MA, USA Department of Neurobiology, Harvard Medical School and Children's Hospital Boston, Boston, MA, USA.
authors.forename: Judith A
authors.initials: JA
authors.lastname: Steen
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D019921:Biotinylation; D015820:Cadherins / Q000378:metabolism; D002846:Chromatography, Affinity; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000502:physiology*; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000378:metabolism*; D008938:Mitosis / Q000502:physiology*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D000091363:Protocadherins; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D015820:Cadherins; D008565:Membrane Proteins; C113391:PCDH7 protein, human; D020543:Proteome; D000091363:Protocadherins
keywords: PCDH7; SILAC; cell cycle; cell rounding; cell surface