title: Strategies for solid-state NMR investigations of supramolecular assemblies with large subunit sizes.
abstract: Solid-state NMR is a versatile tool to study structure and dynamics of insoluble and non-crystalline biopolymers. Supramolecular protein assemblies are formed by self-association of multiple copies of single small-sized proteins. Because of their high degree of local order, solid-state NMR spectra of such systems exhibit an unusually high level of resolution, rendering them an ideal target for solid-state NMR investigations. Recently, our group has solved the structure of one particular supramolecular assembly, the type-iii-secretion-system needle. The needle subunit comprises around 80 residues. Many interesting supramolecular assemblies with unknown structure have subunits larger in size, which requires development of tailored solid-state NMR strategies to address their structures. In this "Perspective" article, we provide a view on different approaches to enhance sensitivity and resolution in biological solid-state NMR with a focus on the possible application to supramolecular assemblies with large subunit sizes.
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Fricke
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Veniamin
authors.initials: V
authors.lastname: Chevelkov
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany.
authors.forename: Chaowei
authors.initials: C
authors.lastname: Shi
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. Electronic address: alange@fmp-berlin.de.
authors.forename: Adam
authors.initials: A
authors.lastname: Lange
mesh_terms: D000465:Algorithms; D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry*; D046912:Multiprotein Complexes / Q000032:analysis* / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D021122:Protein Subunits
chemical_list: D000596:Amino Acids; D046912:Multiprotein Complexes; D021122:Protein Subunits
keywords: Dynamic nuclear polarization; Homonuclear recoupling; Proton detection; Supramolecular protein assemblies