title: Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.
abstract: Previous studies have demonstrated that Shadoo (Sho), a GPI-linked glycoprotein encoded by the Sprn gene with a membrane localization similar to PrP(C), is reduced in the brains of rodents with terminal prion disease. To determine the functional significance of Sho in prion disease pathogenesis, Sho-deficient mice were generated by gene targeting. Sho knockout and control wild-type (WT) mice were infected with themouse-adapted scrapie strains 22L or RML. No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice. In this model of prion disease, Sho removal had no effect on disease pathogenesis.
authors.affiliation: a Institute of Military Veterinary; Academy of Military Medical Science ; Changchun , China.
authors.forename: Sha
authors.initials: S
authors.lastname: Li
authors.forename: Chuanjing
authors.initials: C
authors.lastname: Ju
authors.forename: Chao
authors.initials: C
authors.lastname: Han
authors.forename: Zhongyi
authors.initials: Z
authors.lastname: Li
authors.forename: Wensen
authors.initials: W
authors.lastname: Liu
authors.forename: Xuemin
authors.initials: X
authors.lastname: Ye
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Liang
authors.initials: L
authors.lastname: Xulong
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.forename: Zhibao
authors.initials: Z
authors.lastname: Chen
authors.forename: Keyin
authors.initials: K
authors.lastname: Meng
authors.forename: Jiayu
authors.initials: J
authors.lastname: Wan
mesh_terms: D000818:Animals; D058851:GPI-Linked Proteins; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000502:physiology*; D012608:Scrapie / Q000235:genetics / Q000503:physiopathology*; D015996:Survival Rate
chemical_list: D058851:GPI-Linked Proteins; D009419:Nerve Tissue Proteins; C523856:Sprn protein, mouse
keywords: Shadoo; pathogenesis; prion disease