title: Effect of Porphyromonas gingivalis lipopolysaccharide on bone marrow mesenchymal stem cell osteogenesis on a titanium nanosurface.
abstract: BACKGROUND
  Titanium (Ti) dental implants have been widely used for prosthetic reconstruction of dentition. Unfortunately, peri-implantitis can result in failure of dental implant osseointegration. Lipopolysaccharide (LPS) acts as a chronic inflammatory stimulus and maintains peri-implant inflammation, worsening the prognosis for implant osseointegration. The purpose of this study is to determine the effects of 10 M NaOH-modified Ti surface with nanonetwork structure on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMMSCs) in the context of Porphyromonas gingivalis LPS exposure.
  
  
  METHODS
  Titanium disks treated with 10 M NaOH solution and control were incubated with BMMSCs and exposed to P. gingivalis LPS (0, 0.1, or 1 μg/mL). The effects of the modified nanonetwork structure on osteogenic differentiation of rat BMMSCs were evaluated in the context of different concentrations of P. gingivalis LPS exposure.
  
  
  RESULTS
  Rat BMMSCs on the 10 M NaOH-modified Ti surface with nanonetwork structure had higher levels of osteogenesis-related gene expression and significantly greater cell proliferation, alkaline phosphatase activity, and extracellular matrix deposition and mineralization than cells on the untreated Ti surfaces, in all the groups with different doses of P. gingivalis LPS exposure.
  
  
  CONCLUSIONS
  The 10 M NaOH-modified Ti surface with nanonetwork structure has better endotoxin tolerance under P. gingivalis LPS exposure than the non-modified surface.
authors.affiliation: Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, Xi'an, China.
authors.forename: Helin
authors.initials: H
authors.lastname: Xing
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Taguchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Komasa
authors.forename: Isao
authors.initials: I
authors.lastname: Yamawaki
authors.forename: Tohru
authors.initials: T
authors.lastname: Sekino
authors.forename: Makoto
authors.initials: M
authors.lastname: Umeda
authors.forename: Joji
authors.initials: J
authors.lastname: Okazaki
mesh_terms: D000327:Adsorption; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002113:Calcification, Physiologic / Q000502:physiology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis; D003764:Dental Materials / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000032:analysis; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D049329:Nanostructures / Q000737:chemistry*; D015675:Osteocalcin / Q000032:analysis; D010012:Osteogenesis / Q000187:drug effects*; D015676:Osteonectin / Q000032:analysis; D016966:Porphyromonas gingivalis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012972:Sodium Hydroxide / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D003764:Dental Materials; D016326:Extracellular Matrix Proteins; C546873:Ibsp protein, rat; D058574:Integrin-Binding Sialoprotein; D008070:Lipopolysaccharides; D015676:Osteonectin; C493717:Runx2 protein, rat; D015675:Osteocalcin; D012972:Sodium Hydroxide; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: Lipopolysaccharides; Porphyromonas gingivalis; mesenchymal stromal cells; nanostructures; osseointegration; titanium