title: Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov., thermophilic anaerobic representatives of the phylum Planctomycetes.
abstract: Two novel strains of thermophilic planctomycetes were recovered from terrestrial and subterranean habitats. Strain R1(T) was isolated from a hot spring (Kunashir Island, Russia) and strain SBP2(T) was isolated from a deep gold mine (South Africa). Both isolates grew in the temperature range 30-60 °C and pH range 5.0-8.0. Strain R1(T) grew optimally at 60 °C and pH 6.0-6.5; for SBP2(T) optimal conditions were at 52 °C and pH 7.5-8.0. Both strains were capable of anaerobic respiration with nitrate and nitrite as electron acceptors as well as of microaerobic growth. They also could grow by fermentation of mono-, di- and polysaccharides. Based on their phylogenetic position and phenotypic features we suggest that the new isolates represent two novel species belonging to a new genus in the order Planctomycetales, for which the names Thermogutta terrifontis gen. nov., sp. nov. and Thermogutta hypogea sp. nov. are proposed. The type strain of Thermogutta terrifontis, the type species of the genus, is R1(T) ( = DSM 26237(T) = VKM B-2805(T)), and the type strain of Thermogutta hypogea is SBP2(T) ( = JCM 19991(T) = VKM B-2782(T)).
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Galina B
authors.initials: GB
authors.lastname: Slobodkina
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Olga L
authors.initials: OL
authors.lastname: Kovaleva
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Margarita L
authors.initials: ML
authors.lastname: Miroshnichenko
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Slobodkin
authors.affiliation: Centre 'Bioengineering', Russian Academy of Sciences, Moscow, Russia.
authors.forename: Tatyana V
authors.initials: TV
authors.lastname: Kolganova
authors.affiliation: Gubkin Russian State University of Oil and Gas, Moscow, Russia.
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Novikov
authors.affiliation: University of Free State, Bloemfontein, South Africa.
authors.forename: Esta
authors.initials: E
authors.lastname: van Heerden
authors.affiliation: Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D013019:South Africa; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S