title: A lectin with antifungal activity from the mussel Crenomytilus grayanus.
abstract: Lectins (carbohydrate-binding proteins) are well known to actively participate in the defense functions of vertebrates and invertebrates where they play an important role in the recognition of foreign particles. In this study, we investigated of in vitro antifungal activity of lectin from the mussel Crenomytilus grayanus (CGL). Enzyme-linked immunosorbent assay indicated that CGL was predominantly detectable in tissues of mantle and to a lesser degree in the tissues of muscle, hepatopancreas, gill and hemocytes. After challenged by Pichia pastoris the level of CGL was upregulated and reached the maximum level at 12 h post challenge and recovered to the original level at 24 h. The lectin was capable of inhibiting the germination of spores and hyphal growth in the fungi. All these results indicated that CGL is involved in the innate immune response in mollusc animals.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia; Far Eastern Federal University, 8, Sukhanova str., Vladivostok 690950, Russia.
authors.forename: Irina V
authors.initials: IV
authors.lastname: Chikalovets
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia. Electronic address: chernikovoleg@gmail.com.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Chernikov
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Pivkin
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
authors.forename: Valentina I
authors.initials: VI
authors.lastname: Molchanova
authors.affiliation: Far Eastern Federal University, 8, Sukhanova str., Vladivostok 690950, Russia.
authors.forename: Alina P
authors.initials: AP
authors.lastname: Litovchenko
authors.affiliation: College of Food Science and Engineering, Dalian Ocean University, 52, Heishijiaojie, Shahekou District, Dalian, Liaoning, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
authors.forename: Pavel A
authors.initials: PA
authors.lastname: Lukyanov
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000378:metabolism; D019004:Galanin / Q000235:genetics* / Q000378:metabolism; D037102:Lectins / Q000235:genetics* / Q000378:metabolism; D049879:Mytilidae / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D009928:Organ Specificity; D010843:Pichia / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D037102:Lectins; D019004:Galanin
keywords: Antifungal activity; Crenomytilus grayanus; GalNAc/Gal-specific lectin; Innate immunity; Lectin expression