title: Arabidopsis cold shock domain protein 2 influences ABA accumulation in seed and negatively regulates germination.
abstract: The cold shock domain (CSD) is the most conserved nucleic acid binding domain and is distributed from bacteria to animals and plants. CSD proteins are RNA chaperones that destabilize RNA secondary structures to regulate stress tolerance and development. AtCSP2 is one of the four CSD proteins in Arabidopsis and is up-regulated in response to cold. Since AtCSP2 negatively regulates freezing tolerance, it was proposed to be a modulator of freezing tolerance during cold acclimation. Here, we examined the function of AtCSP2 in seed germination. We found that AtCSP2-overexpressing lines demonstrated retarded germination as compared with the wild type, with or without stress treatments. The ABA levels in AtCSP2-overexpressing seeds were higher than those in the wild type. In addition, overexpression of AtCSP2 reduced the expression of an ABA catabolic gene (CYP707A2) and gibberellin biosynthesis genes (GA20ox and GA3ox). These results suggest that AtCSP2 negatively regulates seed germination by controlling ABA and GA levels.
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Sasaki
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Center for Plant Aging Research, Institute for Basic Science (IBS), Daegu 711-873, Republic of Korea.
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Kim
authors.affiliation: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kanno
authors.affiliation: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Seo
authors.affiliation: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan; Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan. Electronic address: rzi@affrc.go.jp.
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
mesh_terms: D000040:Abscisic Acid / Q000737:chemistry*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D057773:Cold Shock Proteins and Peptides / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018525:Germination; D005875:Gibberellins / Q000737:chemistry; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010940:Plant Proteins; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D018749:RNA, Plant / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D057773:Cold Shock Proteins and Peptides; C521041:GRP2 protein, Arabidopsis; D005875:Gibberellins; D010940:Plant Proteins; D018749:RNA, Plant; D016601:RNA-Binding Proteins; D000040:Abscisic Acid; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C107203:abscisic acid 8'-hydroxylase
keywords: ABA; Arabidopsis; Cold shock domain protein; Gibberellin; Seed germination