title: Magnetic tweezers-based 3D microchannel electroporation for high-throughput gene transfection in living cells.
abstract: A novel high-throughput magnetic tweezers-based 3D microchannel electroporation system capable of transfecting 40 000 cells/cm(2) on a single chip for gene therapy, regenerative medicine, and intracellular detection of target mRNA for screening cellular heterogeneity is reported. A single cell or an ordered array of individual cells are remotely guided by programmable magnetic fields to poration sites with high (>90%) cell alignment efficiency to enable various transfection reagents to be delivered simultaneously into the cells. The present technique, in contrast to the conventional vacuum-based approach, is significantly gentler on the cellular membrane yielding >90% cell viability and, moreover, allows transfected cells to be transported for further analysis. Illustrating the versatility of the system, the GATA2 molecular beacon is delivered into leukemia cells to detect the regulation level of the GATA2 gene that is associated with the initiation of leukemia. The uniform delivery and a sharp contrast of fluorescence intensity between GATA2 positive and negative cells demonstrate key aspects of the platform for gene transfer, screening and detection of targeted intracellular markers in living cells.
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Lingqian
authors.initials: L
authors.lastname: Chang
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Marci
authors.initials: M
authors.lastname: Howdyshell
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Wei-Ching
authors.initials: WC
authors.lastname: Liao
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Chi-Ling
authors.initials: CL
authors.lastname: Chiang
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gallego-Perez
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Wu
authors.initials: W
authors.lastname: Lu
authors.affiliation: Comprehensive Cancer Center, The Ohio State University, Columbus, OH, 43209, USA. Division of Hematology, The Comprehensive Cancer Center, Ohio State University, Columbus, OH, 43209, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Byrd
authors.affiliation: Division of Hematology, The Comprehensive Cancer Center, Ohio State University, Columbus, OH, 43209, USA.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Muthusamy
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: L James
authors.initials: LJ
authors.lastname: Lee
authors.affiliation: NSEC Center for Affordable Nanoengineering of Polymeric Biomedical Devices, Ohio State University, Columbus, OH 43209, USA.
authors.forename: Ratnasingham
authors.initials: R
authors.lastname: Sooryakumar
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D060526:Magnetic Fields; D059346:Magnets; D052898:Optical Tweezers; D014162:Transfection / Q000295:instrumentation* / Q000379:methods
chemical_list: D004247:DNA
keywords: arrays; electroporation; gene delivery; magnetic tweezers; microchannels