title: Isolation of microalgae tolerant to polybrominated diphenyl ethers (PBDEs) from wastewater treatment plants and their removal ability.
abstract: The present study isolated microalgae with tolerance to polybrominated diphenyl ethers (PBDEs) from wastewater aiming to discover isolates with high removal abilities. Nine isolates, Chlorella (STCh and SICh), Parachlorella (STPa1 and STPa2), Scenedesmus (STSc, TPSc1 and TPSc2), Nitzschia palea (YLBa) and Mychonastes (TPMy), were obtained. Four isolates, SICh, STCh, STPa1 and TPSc1, were very tolerant, and their growth was not affected by DE-71 and BDE-209 mixtures (5:1) at low (6 μg L(-1)), medium (60 μg L(-1)) or even high (600 μg L(-1)) levels for 7 days. The removal of PBDEs by one of the tolerant isolates, SICh, was the highest, with 82-90% removal at the end of 7-days exposure. SICh also accumulated more PBDEs than the other isolates. Bioaccumulation and biotransformation were important for PBDE removal. This is the first study isolated PBDE-tolerant microalgae from wastewater and obtained a Chlorella isolate, SICh, with high tolerance and removal ability.
authors.affiliation: Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region.
authors.forename: Dan
authors.initials: D
authors.lastname: Deng
authors.affiliation: Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region; State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region. Electronic address: bhntam@cityu.edu.hk.
authors.forename: Nora Fung-yee
authors.initials: NF
authors.lastname: Tam
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D002452:Cell Count; D002734:Chlorophyll / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D055768:Halogenated Diphenyl Ethers / Q000302:isolation & purification* / Q000633:toxicity*; D058086:Microalgae / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D010802:Phylogeny; D025341:Principal Component Analysis; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical; D018508:Water Purification
chemical_list: D002734:Chlorophyll; D004275:DNA, Ribosomal; D055768:Halogenated Diphenyl Ethers; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Microalgal isolate; PBDEs; Removal; Resistance; Wastewater treatment plant