title: Stimulatory effect of plasma samples from fattening cattle on adipogenesis-related gene expression in preadipocyte cells.
abstract: It is desirable to produce beef with high levels of monounsaturated fatty acids (MUFA), as this is related to fat softness and palatability. However, the physiology of MUFA synthesis in bovine fat during the fattening process remains to be established. In this study, in order to elucidate the relationship between plasma components and the fatty acid composition of intramuscular fat, we investigated the effect of plasma obtained from fattening cattle on the messenger RNA (mRNA) expressions of the adipogenesis-related gene in a clonal bovine intramuscular preadipocyte line (BIP cells). The mRNA expressions of stearoyl-CoA desaturase, adipocyte Protein 2, peroxisome proliferator-activated receptor gamma and sterol regulatory element-binding protein 1 in BIP cells were significantly higher following treatment with those plasma samples collected from the cattle with the highest diaphragmatic unsaturated fatty acids to saturated fatty acids ratio (US/S). Furthermore, the concentration of nonesterified fatty acid (NEFA) in the plasma samples had an inverse correlation with carcass diaphragmatic US/S. These results indicate that cattle with a low ratio of US/S in fat may be discriminated from the population of fattening cattle before slaughter by measuring the effect of their plasma on gene expression in BIP cells as well as their plasma concentration and composition of NEFA.
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Xiangning
authors.initials: X
authors.lastname: Chen
authors.affiliation: Yamagata General Agricultural Research Center, Department of Livestock Science, Shinjo, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Shoji
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Saito
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sumiyoshi
authors.affiliation: Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Rose
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Natumi
authors.initials: N
authors.lastname: Okada
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Cellar Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle / Q000235:genetics* / Q000378:metabolism*; D003964:Diaphragm / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D063427:Food Quality; D015870:Gene Expression; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D000069466:Red Meat; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D047495:PPAR gamma; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1; D013230:Stearoyl-CoA Desaturase
keywords: BIP cell; NEFA; adipogenesis; meat quality; unsaturated fatty acid