title: Leukotriene production profiles and actions in the bovine endometrium during the oestrous cycle.
abstract: We have previously shown the influence of leukotrienes (LTs) on reproductive functions in vivo: LTB4 is luteotrophic and supports corpus luteum function inducing PGE2 and progesterone (P4) secretion, whereas LTC4 is luteolytic and stimulates PGF2α secretion in cattle. The aim of this study was to examine expression and production profiles of LTs and their actions in the endometrium. LT receptors (LTB4R for LTB4 and CysLTR2 for LTC4), 5-lipoxygenase (LO), 12-LO synthase (LTCS) and LTA4 hydrolase (LTAH) mRNA and protein expression, as well as LT production were measured in bovine endometrial tissue during the luteal phases of the oestrous cycle. The action of LTs on uterine function was studied by measuring the level of PGs after stimulating uterine slices with LTs on Days 8-10 of the cycle. Expression of 5-LO and LTB4R mRNA and protein were highest on Days 2-4 of the cycle, while CysLTR2 and LTCS were highest on Days 16-18 (P<0.05). LTB4 concentration was highest on Days 2-4 of the cycle, whereas the greatest LTC4 level was on Days 16-18 (P<0.05). Both LTB4 and C4 increased the content of PGE2 and F2α in endometrial slices at a dose of 10(-7)M (P<0.05). In summary, mRNA expression and activation of receptors for LTB4 and production occur in the first part of the cycle, whereas LTC4 and its receptors predominate at the end of the cycle. The 12-LO and 5-LO pathways are complementary routes of LT production in the bovine uterus.
authors.affiliation: Department of Reproductive Immunology and Pathology of the Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 St., 10-747 Olsztyn, Poland.
authors.forename: Anna J
authors.initials: AJ
authors.lastname: Korzekwa
authors.affiliation: Department of Statistics and Medical Informatics, Medical University of Bialystok, Szpitalna 37 St., 15-295 Bialystok, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Milewski
authors.affiliation: Department of Reproductive Immunology and Pathology of the Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 St., 10-747 Olsztyn, Poland.
authors.forename: Martyna
authors.initials: M
authors.lastname: Łupicka
authors.affiliation: Department of Reproductive Immunology and Pathology of the Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 St., 10-747 Olsztyn, Poland.
authors.forename: Dariusz J
authors.initials: DJ
authors.lastname: Skarzynski
mesh_terms: D000003:Abattoirs; D000818:Animals; D017849:Animals, Inbred Strains; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D003612:Dairying; D004717:Endometrium / Q000201:enzymology / Q000378:metabolism*; D030762:Estrous Cycle / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D015971:Gene Expression Regulation, Enzymologic; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D007975:Leukotriene B4 / Q000378:metabolism; D017997:Leukotriene C4 / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism*; D008183:Luteal Phase / Q000378:metabolism*; D011044:Poland; D011453:Prostaglandins / Q000819:agonists / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018077:Receptors, Leukotriene / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D018102:Receptors, Leukotriene B4 / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D015289:Leukotrienes; D011453:Prostaglandins; D012333:RNA, Messenger; D018077:Receptors, Leukotriene; D018102:Receptors, Leukotriene B4; D007975:Leukotriene B4; D017997:Leukotriene C4; C412375:cysteinyl leukotriene receptor 2; D001094:Arachidonate 5-Lipoxygenase; D005982:Glutathione Transferase; C041116:leukotriene-C4 synthase