title: Oleanolic acid suppresses the proliferation of lung carcinoma cells by miR-122/Cyclin G1/MEF2D axis.
abstract: Oleanolic acid (OA) is a natural compound from plants with anti-tumor activities. However, the mechanism of the inhibitory effect of OA on cell cycle progression has not been completely explored. We employed several lung carcinoma cell lines to investigate the cell cycle-related molecular pathway affected by OA. The data revealed that OA suppressed the proliferation of lung cancer cells in both dose- and time-dependent manners, along with an increase in miR-122 abundance. The suppression of miR-122 abolished the effect of OA on lung cancer cells. CCNG1 and MEF2D, two putative miR-122 targets, were found to be downregulated by OA treatment. Restoring their expression counteracted the effect of OA on lung carcinoma cells. OA was further shown to induce the expression of miR-122-regulating transcriptional factors in lung cancer cells. Collectively, OA induced cell cycle arrest in lung cancer cells through miR-122/Cyclin G1/MEF2D pathway. This finding may contribute to the understanding of the molecular mechanism of OA's anti-tumor activity.
authors.affiliation: Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, 440 Jiyan Road, Jinan, 250117, Shandong, People's Republic of China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Zhao
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Daotang
authors.initials: D
authors.lastname: Li
mesh_terms: D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D056766:Cyclin G1 / Q000096:biosynthesis* / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D064326:MEF2 Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009828:Oleanolic Acid / Q000008:administration & dosage*
chemical_list: C534702:CCNG1 protein, human; D056766:Cyclin G1; D064326:MEF2 Transcription Factors; C578043:MEF2D protein, human; C531907:MIRN122 microRNA, human; D035683:MicroRNAs; D009828:Oleanolic Acid