title: In or out? On the tightness of glycosomal compartmentalization of metabolites and enzymes in Trypanosoma brucei.
abstract: Trypanosomatids sequester large parts of glucose metabolism inside specialised peroxisomes, called glycosomes. Many studies have shown that correct glycosomal compartmentalization of glycolytic enzymes is essential for bloodstream-form Trypanosoma brucei. The recent finding of pore-forming activities in glycosomal membrane preparations and extensions of the trypanosome glycolysis computer model with size-selective pores sparked again an old debate on the extent of (im)permeability of the glycosomal membrane and whether glycosomally located glycolytic enzymes could and should also be present with some activity in the cytosol. This review presents a critical discussion of the experimental and theoretical evidence for and against the different hypotheses.
authors.affiliation: Department of Pediatrics and Systems Biology Centre for Energy Metabolism and Ageing, Centre for Liver, Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen, The Netherlands; Department of Molecular Cell Physiology, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, The Netherlands. Electronic address: j.r.haanstra@vu.nl.
authors.forename: Jurgen R
authors.initials: JR
authors.lastname: Haanstra
authors.affiliation: Department of Pediatrics and Systems Biology Centre for Energy Metabolism and Ageing, Centre for Liver, Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen, The Netherlands; Department of Molecular Cell Physiology, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, The Netherlands.
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Bakker
authors.affiliation: Institute of Structural and Molecular Biology, School of Biological Sciences, University of Edinburgh, UK; Laboratorio de Enzimología de Parásitos, Departamento de Biología, Universidad de Los Andes, Mérida, Venezuela.
authors.forename: Paul A M
authors.initials: PA
authors.lastname: Michels
mesh_terms: D000818:Animals; D001692:Biological Transport; D006019:Glycolysis; D006801:Humans; D008830:Microbodies / Q000378:metabolism / Q000469:parasitology*; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014353:Trypanosomiasis, African / Q000378:metabolism* / Q000469:parasitology
chemical_list: D015800:Protozoan Proteins
keywords: Compartmentalization of metabolism; Glycolysis; Glycosomes; Pores