title: Mass transfer coefficients determination from linear gradient elution experiments.
abstract: A procedure to estimate mass transfer coefficients in linear gradient elution chromatography is presented and validated by comparison with experimental data. Mass transfer coefficients are traditionally estimated experimentally through the van Deemter plot, which represents the HETP as a function of the fluid velocity. Up to now, the HETP was obtained under isocratic elution conditions. Unfortunately, isocratic elution experiments are often not suitable for large biomolecules which suffer from severe mass transfer hindrances. Yamamoto et al. were the first to propose a semi-empirical equation to relate HETPs measured from linear gradient elution experiments to those obtained under isocratic conditions [7]. Based on his pioneering work, the approach presented in this work aims at providing an experimental procedure supported by simple equations to estimate reliable mass transfer parameters from linear gradient elution chromatographic experiments. From the resolution of the transport model, we derived a rigorous analytical expression for the HETP in linear gradient elution chromatography.
authors.affiliation: Institute for Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Pfister
authors.affiliation: Institute for Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 8093 Zurich, Switzerland. Electronic address: massimo.morbidelli@chem.ethz.ch.
authors.forename: Massimo
authors.initials: M
authors.lastname: Morbidelli
mesh_terms: D002845:Chromatography / Q000379:methods*; D008956:Models, Chemical; D011506:Proteins / Q000737:chemistry
chemical_list: D011506:Proteins
keywords: Chromatography model; Linear gradient elution; Mass transfer coefficient; Parameter estimation