title: The establishment of the infant intestinal microbiome is not affected by rotavirus vaccination.
abstract: The microbial colonization of the intestine during the first months of life constitutes the most important process for the microbiota-induced host-homeostasis. Alterations in this process may entail a high-risk for disease in later life. However, the potential factors affecting this process in the infant are not well known. Moreover, the potential impact of orally administered vaccines upon the establishing microbiome remains unknown. Here we assessed the intestinal microbiome establishment process and evaluated the impact of rotavirus vaccination upon this process. Metagenomic, PCR-DGGE and faecal short chain fatty acids analyses were performed on faecal samples obtained from three infants before and after the administration of each dose of vaccine. We found a high inter-individual variability in the early life gut microbiota at microbial composition level, but a large similarity between the infants' microbiomes at functional level. Rotavirus vaccination did not show any major effects upon the infant gut microbiota. Thus, the individual microbiome establishment and development process seems to occur in a defined manner during the first stages of life and rotavirus vaccination appears to be inconsequential for this process.
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University, Hangzhou, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ang
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Arboleya
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University, Hangzhou, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Lihua
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University, Hangzhou, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chuihui
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, Zhejiang University, Hangzhou, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Nan
authors.affiliation: Pediatrics Service, Hospital Universitario Central de Asturias, SESPA, Oviedo, Asturias, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Suarez
authors.affiliation: Pediatrics Service, Hospital Universitario Central de Asturias, SESPA, Oviedo, Asturias, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Solís
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Clara G
authors.initials: CG
authors.lastname: de los Reyes-Gavilán
authors.affiliation: Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Villaviciosa, Asturias, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gueimonde
mesh_terms: D006801:Humans; D007223:Infant; D007422:Intestines / Q000382:microbiology*; D008297:Male; D056186:Metagenomics / Q000379:methods*; D064307:Microbiota / Q000502:physiology*; D022243:Rotavirus Vaccines / Q000008:administration & dosage* / Q000009:adverse effects; D014611:Vaccination
chemical_list: D022243:Rotavirus Vaccines