title: Avian area vasculosa and CAM as rapid in vivo pro-angiogenic and antiangiogenic models.
abstract: Angiogenesis, the development of new blood vessels from preexisting ones, is driven by coordinated signaling pathways governed by specific molecules, hemodynamic forces, and endothelial and periendothelial cells. The processes involve adhesion, migration, and survival machinery within the target endothelial and periendothelial cells. Factors that interfere with any of these processes may therefore influence angiogenesis either positively (pro-angiogenesis) or negatively (antiangiogenesis). The avian area vasculosa (AV) and the avian chorioallantoic membrane (CAM) are two useful tools for studying both angiogenesis and antiangiogenesis since they are amenable to both intravascular and topical administration of target, agents, are relatively rapid assays, and can be adapted very easily to study angiogenesis-dependent processes, such as tumor growth. Both models provide a physiological setting that permits investigation of pro-angiogenic and antiangiogenic agent interactions in vivo.
authors.affiliation: Institute of Anatomy, University of Bern, Baltzerstrasse 2, 3000, Bern 9, Switzerland, andrew.makanya@ana.unibe.ch.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Makanya
authors.forename: Beata
authors.initials: B
authors.lastname: Styp-Rekowska
authors.forename: Ivanka
authors.initials: I
authors.lastname: Dimova
authors.forename: Valentin
authors.initials: V
authors.lastname: Djonov
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply* / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D023421:Models, Animal; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010793:Phthalazines / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D046509:Tissue Culture Techniques; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors; D015017:Yolk Sac / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects
chemical_list: D003911:Dextrans; D010793:Phthalazines; D011480:Protease Inhibitors; D011725:Pyridines; D051880:Receptors, Notch; D042461:Vascular Endothelial Growth Factor A; C015219:fluorescein isothiocyanate dextran; C404768:vatalanib; D053829:Amyloid Precursor Protein Secretases; D016650:Fluorescein-5-isothiocyanate