title: Effects of resveratrol on ovarian response to controlled ovarian hyperstimulation in ob/ob mice.
abstract: OBJECTIVE
  To evaluate antidiabetic and anti-inflammatory effects of resveratrol on the ovarian response to controlled ovarian hyperstimulation (COH) in obesity-related infertility.
  
  
  METHODS
  Experimental.
  
  
  METHODS
  University laboratory.
  
  
  METHODS
  Sixteen female ob/ob mice and 16 female C57BL/6J mice undergoing COH.
  
  
  METHODS
  Wild-type placebo group; wild-type resveratrol group; ob/ob mice placebo group; ob/ob mice resveratrol group. Resveratrol 3.75 mg/kg daily for 20 days and undergoing COH protocol.
  
  
  METHODS
  Body and reproductive system weight, food intake, fasting blood glucose, plasma insulin and T levels, and Homeostatic Index of Insulin Resistance; interleukin-6 and tumor necrosis factor-α levels in adipose tissue by Western blot; assessment of quality and quantity of oocytes retrieved; and quantitative analysis of ovarian follicles.
  
  
  RESULTS
  Plasma insulin and T levels decreased and Homeostatic Index of Insulin Resistance improved in ob/ob mice treated with resveratrol. Interleukin-6 and tumor necrosis factor-α levels were significantly reverted back to near normalcy after resveratrol treatment in obese mice. Administration of resveratrol resulted in a significantly higher number of oocytes collected in wild-type mice. The number of primary, growing, preovulatory, and atretic follicles was found to be decreased in the group of obese mice treated with resveratrol when compared with the obese control group.
  
  
  CONCLUSIONS
  Resveratrol administration could exert benefits against loss of ovarian follicles, and these actions may be mediated, at least in part, via anti-inflammatory, insulin-sensitizing, and antihyperandrogenism effects. These observations further validate the therapeutic potential of resveratrol to preserve ovarian reserve in conditions associated with obesity. Our results suggest the possible clinical use of resveratrol to enhance the ovarian response to COH in normal-weight females.
authors.affiliation: Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Cabello
authors.affiliation: Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Garrido
authors.affiliation: Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Morán
authors.affiliation: Department of Pathology, Asturias Central University Hospital, Oviedo, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: González del Rey
authors.affiliation: Department of Obstetrics and Gynecology, Asturias Central University Hospital, Oviedo, Spain.
authors.forename: Plácido
authors.initials: P
authors.lastname: Llaneza
authors.affiliation: Centro de Fertilización in Vitro de Asturias (CEFIVA), Oviedo, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Llaneza-Suárez
authors.affiliation: Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Alonso
authors.affiliation: Department of Functional Biology, Physiology Area, University of Oviedo, Oviedo, Spain. Electronic address: tinog@uniovi.es.
authors.forename: Celestino
authors.initials: C
authors.lastname: González
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D016471:Ovarian Hyperstimulation Syndrome / Q000188:drug therapy* / Q000378:metabolism*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use*
chemical_list: D001786:Blood Glucose; D013267:Stilbenes; D000077185:Resveratrol
keywords: Obesity; controlled ovarian hyperstimulation; infertility; leptin-deficient mice; ovarian reserve; resveratrol