title: Generation of a syngeneic orthotopic transplant model of prostate cancer metastasis.
abstract: Progression to metastatic disease is the primary cause of mortality in men with prostate cancer (PCa). Mouse models which progress with spontaneous metastasis are limited. Such models would allow for extensive studies of molecular mechanisms of metastasis, and more definite pre- clinical therapy trials. Orthotopic murine models have been described; however a limiting biology of these models is their lack of an intact immune system. Within, we describe the development of an androgen sensitive and castrate resistant tractable orthotopic murine syngeneic (immune competent) model of prostate cancer. Both models develop primary tumors which spontaneously progress to metastatic disease in lymph tissue. These models will allow for more complete mechanistic and therapeutic studies in a short time period.
authors.affiliation: Genitourinary Program, Roswell Park Cancer Institute, Buffalo NY, USA ; Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo NY, USA.
authors.forename: Leigh
authors.initials: L
authors.lastname: Ellis
authors.affiliation: Genitourinary Program, Roswell Park Cancer Institute, Buffalo NY, USA.
authors.forename: Kristin
authors.initials: K
authors.lastname: Lehet
authors.affiliation: Genitourinary Program, Roswell Park Cancer Institute, Buffalo NY, USA ; Department of Cancer Prevention and Pathology, Roswell Park Cancer Institute, Buffalo NY, USA.
authors.forename: ShengYu
authors.initials: S
authors.lastname: Ku
authors.affiliation: Genitourinary Program, Roswell Park Cancer Institute, Buffalo NY, USA ; Department of Pathology, Roswell Park Cancer Institute, Buffalo NY, USA.
authors.forename: Gissou
authors.initials: G
authors.lastname: Azabdaftari
authors.affiliation: Genitourinary Program, Roswell Park Cancer Institute, Buffalo NY, USA ; Department of Medicine, Roswell Park Cancer Institute, Buffalo NY, USA.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pili
keywords: MYC; castrate resistant prostate cancer; metastasis; mouse models; prostate cancer