title: Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton.
abstract: Platelet-rich plasma (PRP) is a platelet concentrate in a small volume of plasma. It is highly enriched in growth factors able to stimulate the migration and growth of bone-forming cells. PRP is often used in clinical applications, as dental surgery and fracture healing. Platelet derived growth factor (PDGF), is highly concentrated in PRP and it was shown in our previous studies to provide the chemotactic stimulus to SaOS-2 osteoblasts to move in a microchemotaxis assay. Aim of the present studies is to analyze the effects of a PRP pretreatment (short time course: 30-150 min) of SaOS-2 cells with PRP on the organization of actin cytoskeleton, the main effector of cell mobility. The results indicate that a pretreatment with PRP increases chemokinesis and chemotaxis and concomitantly induces the organization of actin microfilaments, visualized by immunocytochemistry, in a directionally elongated phenotype, which is characteristic of the cells able to move. PRP also produces a transient increase in the expression of PGDF α receptor. This reorganization is blocked by the immunoneutralization of PDGF demonstrating the responsibility of this growth factor in triggering the mechanisms responsible for cellular movements.
authors.affiliation: a Department di Pharmacological and Biomedical Sciences ; University of Milano ; Milano , Italy.
authors.forename: Lavinia
authors.initials: L
authors.lastname: Casati
authors.forename: Fabio
authors.initials: F
authors.lastname: Celotti
authors.forename: Paola
authors.initials: P
authors.lastname: Negri-Cesi
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Sacchi
authors.forename: Paolo
authors.initials: P
authors.lastname: Castano
authors.forename: Alessandra
authors.initials: A
authors.lastname: Colciago
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002633:Chemotaxis; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D053657:Platelet-Rich Plasma
chemical_list: D010982:Platelet-Derived Growth Factor
keywords: PDGF receptor; PRP; SaOS-2; actin; chemotaxis; cytoskeleton; migration; osteoblasts