title: Alleviated mucosal and neuronal damage in a rat model of Crohn's disease.
abstract: OBJECTIVE
  To establish a rat model suitable to investigate the repetitive relapsing inflammations (RRI) characteristic to Crohn's disease.
  
  
  METHODS
  Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). RRI were mimicked by repeating administrations of TNBS. Tissue samples were taken from control, once, twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation. The means of the ulcerated area were measured to evaluate the macroscopic mucosal damage. The density of myenteric neurons was determined on whole mounts by HuC/HuD immunohistochemistry. Heme oxygenase-1 (HO-1) expression was evaluated by molecular biological techniques.
  
  
  RESULTS
  TNBS-treated rats displayed severe colitis, but the mortality was negligible, and an increase of body weight was characteristic throughout the experimental period. The widespread loss of myenteric neurons, and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration. After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased, and the neuronal loss was on a smaller scale and was limited to the inflamed area. HO-1 mRNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined. Nevertheless, the HO-1 protein up-regulation after the second TNBS treatment proved to be transient. Following the third treatment HO-1 protein expression could not be detected.
  
  
  CONCLUSIONS
  Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage. The persistent up-regulation of HO-1 mRNA expression may correlate with this.
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Petra
authors.initials: P
authors.lastname: Talapka
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Lajos István
authors.initials: LI
authors.lastname: Nagy
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Pál
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Marietta Zita
authors.initials: MZ
authors.lastname: Poles
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Anikó
authors.initials: A
authors.lastname: Berkó
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Bagyánszki
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: László Géza
authors.initials: LG
authors.lastname: Puskás
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Fekete
authors.affiliation: Petra Talapka, Alexandra Pál, Marietta Zita Poles, Anikó Berkó, Mária Bagyánszki, Éva Fekete, Nikolett Bódi, Department of Physiology, Anatomy and Neuroscience, Faculty of Sciences and Informatics, University of Szeged, H-6726 Szeged, Hungary.
authors.forename: Nikolett
authors.initials: N
authors.lastname: Bódi
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D003106:Colon / Q000201:enzymology / Q000294:innervation / Q000473:pathology*; D003424:Crohn Disease / Q000139:chemically induced / Q000201:enzymology / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000378:metabolism; D007413:Intestinal Mucosa / Q000473:pathology*; D008297:Male; D009197:Myenteric Plexus / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D012008:Recurrence; D012074:Remission Induction; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger; D014302:Trinitrobenzenesulfonic Acid; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat
keywords: Crohn’s disease; Experimental rat model; Heme oxygenase-1; Myenteric neurons; Repetitive relapsing inflammation