title: In-vivo optical tomography of small scattering specimens: time-lapse 3D imaging of the head eversion process in Drosophila melanogaster.
abstract: Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques, obtaining images only from subsurface features. This means that in order to obtain 3D volumetric information these specimens need to be studied either after fixation and a chemical clearing process, through an imaging window--thus perturbing physiological development -, or during early stages of development when the scattering contribution is negligible. In this paper we showcase how Optical Projection Tomography may be used to obtain volumetric images of the head eversion process in vivo in Drosophila melanogaster pupae, both in control and headless mutant specimens. Additionally, we demonstrate the use of Helical Optical Projection Tomography (hOPT) as a tool for high throughput 4D-imaging of several specimens simultaneously.
authors.affiliation: Institute for Biomedical Engineering, Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich, Switzerland.
authors.forename: Alicia
authors.initials: A
authors.lastname: Arranz
authors.affiliation: Institute of Automation, Chinese Academy of Sciences, Beijing. 100190, China.
authors.forename: Di
authors.initials: D
authors.lastname: Dong
authors.affiliation: School of Life Sciences and Technology, Xidian University, Xian, Shaanxi 710071, China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Zhu
authors.affiliation: B.S.R.C. Alexander Fleming, Varkiza. 16602, Greece.
authors.forename: Charalambos
authors.initials: C
authors.lastname: Savakis
authors.affiliation: 1] Institute of Automation, Chinese Academy of Sciences, Beijing. 100190, China [2] School of Life Sciences and Technology, Xidian University, Xian, Shaanxi 710071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
authors.affiliation: 1] Department of Bioengineering and Aerospace Engineering, Universidad Carlos III of Madrid, 28911 Madrid, Spain [2] Experimental Medicine and Surgery Unit, Instituto de Investigación Sanitaria del Hospital Gregorio Marañón, 28007 Madrid, Spain.
authors.forename: Jorge
authors.initials: J
authors.lastname: Ripoll
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods; D004331:Drosophila melanogaster / Q000502:physiology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D011679:Pupa / Q000502:physiology; D041622:Tomography, Optical / Q000379:methods*