title: Development of carboxymethyl cellulose nonwoven sheet as a novel hemostatic agent.
abstract: Carboxymethyl cellulose (CMC) is a plant-derived material that has high biocompatibility and water solubility. We developed a CMC nonwoven sheet as a hemostatic agent by carboxymethylating a continuous filament cellulose nonwoven sheet. The CMC nonwoven sheet was able to absorb water and dissolve in it. The rates of absorption and dissolution depended on the degree of carboxymethylation. After dissolving in blood, CMC accelerated clot development (possibly owing to the incorporation of CMC into fibrin fibers) and increased the viscosity of the blood, both of which would contribute to the improved blood clotting of an injured surface. In vivo experiments using a rat tail cutting method showed that a CMC nonwoven sheet shortened the bleeding time of the tail when applied to the cut surface. The hemostatic effect of the CMC nonwoven sheet was almost at the same level as a commercial hemostatic bandage. These results suggest that a CMC nonwoven sheet could be used as a novel sheet-type hemostatic agent.
authors.affiliation: Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ohta
authors.affiliation: Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Department of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-ogino, Atsugi, Kanagawa 243-0292, Japan.
authors.forename: Megumu
authors.initials: M
authors.lastname: Sakoda
authors.affiliation: Asahi Kasei Fibers Corporation, 4-3400-1 Asahi-machi, Nobeoka-shi, Miyazaki 882-0847, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Machioka
authors.affiliation: Asahi Kasei Fibers Corporation, 4-3400-1 Asahi-machi, Nobeoka-shi, Miyazaki 882-0847, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Fuke
authors.affiliation: Department of Applied Bioscience, Kanagawa Institute of Technology, 1030 Shimo-ogino, Atsugi, Kanagawa 243-0292, Japan.
authors.forename: Shigetoshi
authors.initials: S
authors.lastname: Ichimura
authors.affiliation: Department of Surgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Fuyuki
authors.initials: F
authors.lastname: Inagaki
authors.affiliation: Department of Surgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimizu
authors.affiliation: Department of Surgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Hasegawa
authors.affiliation: Department of Surgery, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Kokudo
authors.affiliation: Department of Clinical Laboratory Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kaneko
authors.affiliation: Department of Clinical Laboratory Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yatomi
authors.affiliation: Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: taichi@m.u-tokyo.ac.jp.
authors.forename: Taichi
authors.initials: T
authors.lastname: Ito
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D001458:Bandages; D001760:Bleeding Time; D001777:Blood Coagulation / Q000187:drug effects; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry* / Q000494:pharmacology*; D002482:Cellulose / Q000737:chemistry; D005337:Fibrin / Q000737:chemistry; D006489:Hemostatic Techniques; D006490:Hemostatics / Q000097:blood / Q000737:chemistry* / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013623:Tail / Q000098:blood supply; D014783:Viscosity / Q000187:drug effects; D014867:Water / Q000737:chemistry
chemical_list: D006490:Hemostatics; D014867:Water; D005337:Fibrin; D002482:Cellulose; D002266:Carboxymethylcellulose Sodium
keywords: Bandage; Biomaterial; Blood clotting; Carboxymethyl cellulose; Hemostasis; Nonwoven sheet