title: Multiresidue Determination of β2-Agonists Including Phenylethanolamine A in Animal-Derived Food by Ultra-High Performance Liquid Chromatography/Tandem Mass Spectrometry.
abstract: A comprehensive ultra-high performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) procedure for detection of nine β-agonists in animal-derived food is described. The method was based on enzymatic hydrolysis with β-glucuronidase from Helix pomatia, followed by a liquid-liquid extraction procedure with perchloric acid and a solid-phase extraction scheme using two kinds of cartridges, HLB and MCX. The influence of sample solution pH in the extraction recovery was studied, and pH 4.0 was found to give the best recovery. The analytes were eluted with methanol containing 4% ammonia. A validation procedure for quantitative analysis of β-agonists in animal-derived food was performed. The three kinds of internal standards, d3-salbutamol, d6-ractopamine and d9-clenpenterol, were applied in the sample preparation and detection of UHPLC/MS/MS. The recoveries from spiked samples ranged between 74.9 and 106.9%. The relative standard deviations of detection were at 0.7-9.6%. The limits of detection and quantification were 0.01-0.05 and 0.03-0.20 µg/kg, respectively. The effect of sample matrix in the detection was discussed in detail.
authors.affiliation: National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China Chengdu Centre for Disease Control and Prevention, Chengdu 610041, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Wang
authors.affiliation: West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Rong-chun
authors.initials: RC
authors.lastname: Pu
authors.affiliation: Chengdu Centre for Disease Control and Prevention, Chengdu 610041, China.
authors.forename: Xi-xi
authors.initials: XX
authors.lastname: Wang
authors.affiliation: Chengdu Centre for Disease Control and Prevention, Chengdu 610041, China.
authors.forename: Chun-ying
authors.initials: CY
authors.lastname: Luo
authors.affiliation: Chengdu Centre for Disease Control and Prevention, Chengdu 610041, China.
authors.forename: Li-chun
authors.initials: LC
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China State Key Laboratory of Hydraulics and the Exploitation and Protection of Mountain Rivers, Sichuan University, Chengdu 610065, China zhangxinshen@126.com.
authors.forename: Xin-shen
authors.initials: XS
authors.lastname: Zhang
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D004983:Ethanolamines / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D015203:Reproducibility of Results; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D000318:Adrenergic beta-Agonists; D004983:Ethanolamines; D019155:Veterinary Drugs