title: Composition of polyphenol and polyamide compounds in common ragweed (Ambrosia artemisiifolia L.) pollen and sub-pollen particles.
abstract: Phenolic composition of Ambrosia artemisiifolia L. pollen and sub-pollen particles (SPP) aqueous extracts was determined, using a novel extraction procedure. Total phenolic and flavonoid content was determined, as well as the antioxidative properties of the extract. Main components of water-soluble pollen phenolics are monoglycosides and malonyl-mono- and diglycosides of isorhamnetin, quercetin and kaempferol, while spermidine derivatives were identified as the dominant polyamides. SPP are similar in composition to pollen phenolics (predominant isorhamnetin and quercetin monoglycosides), but lacking small phenolic molecules (<450Da). Ethanol-based extraction protocol revealed one-third lower amount of total phenolics in SPP than in pollen. For the first time in any pollen species, SPP and pollen phenolic compositions were compared in detail, with an UHPLC/ESI-LTQ-Orbitrap-MS-MS approach, revealing the presence of spermidine derivatives in both SPP and pollen, not previously reported in Ambrosia species.
authors.affiliation: Center of Excellence in Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia.
authors.forename: Luka
authors.initials: L
authors.lastname: Mihajlovic
authors.affiliation: Center of Excellence in Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Radosavljevic
authors.affiliation: Institute of Virology, Vaccines and Sera "Torlak", Belgrade, Serbia.
authors.forename: Lidija
authors.initials: L
authors.lastname: Burazer
authors.affiliation: Center of Excellence in Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia.
authors.forename: Katarina
authors.initials: K
authors.lastname: Smiljanic
authors.affiliation: Center of Excellence in Molecular Food Sciences, University of Belgrade - Faculty of Chemistry, Belgrade, Serbia. Electronic address: tcirkov@chem.bg.ac.rs.
authors.forename: Tanja
authors.initials: T
authors.lastname: Cirkovic Velickovic
mesh_terms: D031164:Ambrosia / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry; D044949:Kaempferols / Q000737:chemistry; D015394:Molecular Structure; D009757:Nylons / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D011058:Pollen / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry; D013095:Spermidine / Q000737:chemistry
chemical_list: D000975:Antioxidants; D044949:Kaempferols; D009757:Nylons; D010936:Plant Extracts; D059808:Polyphenols; C047368:3-methylquercetin; C006552:kaempferol; D011794:Quercetin; D013095:Spermidine
keywords: Ambrosia; Common ragweed; Flavonoids; Immunomodulatory role; Pollen; Polyamides; Polyphenolics; Sub-pollen particles; UHPLC/ESI-LTQ-Orbitrap-MS–MS