title: Combination of CD160 and CD200 as a useful tool for differential diagnosis between chronic lymphocytic leukemia and other mature B-cell neoplasms.
abstract: BACKGROUND
  Chronic lymphocytic leukemia is usually diagnosed through the characteristic morphology/immunophenotype of the lymphocytes, but some CLL cases remain atypical resulting in diagnostic uncertainty.
  
  
  METHODS
  Using flow cytometry analysis, we investigated the expression of CDs160/200 on B cells from 124 patients (82 CLL, 42 other B-cell neoplasms) and nine controls. CDs160/200 measurements were determined as a ratio of the mean fluorescence intensities of leukemic cells/controls and were considered positive when the ratios were ≥2 and 20, respectively.
  
  
  RESULTS
  Sixty and 83% CLL expressed CDs160/200 as compared to 5% and 10% of other B-cell neoplasms, respectively. None of the controls showed CDs160/200 expressions. Combination of both markers was observed in 55% of CLL but only in 2% of other B-cell neoplasms, and absence of both markers occurred in 12% of CLL but in 86% of other B-cell neoplasms.
  
  
  CONCLUSIONS
  CDs160/200 were associated with markers of the gold standard 'Matutes score' and could be useful markers to differentiate atypical CLL from other B-cell neoplasms in the absence of available biopsies or cytogenetics and molecular studies.
authors.affiliation: Laboratory of Hematology, University Hospital, Nancy, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Lesesve
authors.affiliation: Laboratory of Hematology, University Hospital, Nancy, France.|Department of Hematology, University Hospital, Nancy, France.
authors.forename: S
authors.initials: S
authors.lastname: Tardy
authors.affiliation: Laboratory of Hematology, University Hospital, Nancy, France.
authors.forename: B
authors.initials: B
authors.lastname: Frotscher
authors.affiliation: Laboratory of Hematology, University Hospital, Nancy, France.
authors.forename: V
authors.initials: V
authors.lastname: Latger-Cannard
authors.affiliation: Department of Hematology, University Hospital, Nancy, France.
authors.forename: P
authors.initials: P
authors.lastname: Feugier
authors.affiliation: Laboratory of Immunology, University Hospital, Nancy, France.
authors.forename: M
authors.initials: M
authors.lastname: De Carvalho Bittencourt
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000235:genetics* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D003937:Diagnosis, Differential; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008218:Lymphocytosis / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D018442:Lymphoma, B-Cell, Marginal Zone / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008224:Lymphoma, Follicular / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D020522:Lymphoma, Mantle-Cell / Q000175:diagnosis / Q000235:genetics / Q000276:immunology / Q000473:pathology; D008875:Middle Aged; D011237:Predictive Value of Tests; D012372:ROC Curve; D011971:Receptors, Immunologic / Q000235:genetics* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; C114505:CD160 protein, human; D058851:GPI-Linked Proteins; D011971:Receptors, Immunologic; C042739:antigens, CD200
keywords: B-cell neoplasms; CD160; CD200; CLL; immunophenotyping