title: Prediction of Fetal Growth Restriction by Analyzing the Messenger RNAs of Angiogenic Factor in the Plasma of Pregnant Women.
abstract: OBJECTIVE
  To predict the occurrence of fetal growth restriction (FGR) by analyzing messenger RNA (mRNA) expression levels of vascular endothelial growth factor receptor 1 (fms-like tyrosine kinase 1 [Flt-1]) in maternal blood.
  
  
  METHODS
  Eleven women with FGR were matched with 88 controls. Plasma samples were obtained during each trimester. The Flt-1 mRNA expression levels were compared between groups. Predicted probabilities were calculated, and sensitivity-specificity (receiver-operating characteristic [ROC]) curves were assessed based on regression models for each trimester measurement and possible combinations of measurements.
  
  
  RESULTS
  The mRNA levels of the FGR group during all trimesters were significantly higher than those of the control group. The ROC curve of combined first and second trimester data yielded a detection rate of 60% at a 10% false-positive rate, with an area under curve of 0.79.
  
  
  CONCLUSIONS
  The Flt-1 mRNA expression in maternal blood can be used as a marker to predict the development of FGR, long before a clinical diagnosis is made.
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan shin-take@med.showa-u.ac.jp.
authors.forename: Shin
authors.initials: S
authors.lastname: Takenaka
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan Fetal Medicine Unit, Instituto Nacional Materno Perinatal, Lima, Peru.
authors.forename: Walter
authors.initials: W
authors.lastname: Ventura
authors.affiliation: Department of Statistical Sciences, University of Bologna, Bologna, Italy.
authors.forename: Anna Freni
authors.initials: AF
authors.lastname: Sterrantino
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kawashima
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Koide
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Hori
authors.affiliation: Department of Medicine and Surgery (DIMEC), Division of Prenatal Medicine, University of Bologna, Bologna, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Farina
authors.affiliation: Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
mesh_terms: D000328:Adult; D019540:Area Under Curve; D016022:Case-Control Studies; D042241:Early Diagnosis; D005189:False Positive Reactions; D005260:Female; D005317:Fetal Growth Retardation / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008137:Longitudinal Studies; D011237:Predictive Value of Tests; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics*; D012372:ROC Curve; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000097:blood / Q000235:genetics*; D055815:Young Adult
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1
keywords: fetal growth restriction; messenger RNA; small for gestational age; vascular endothelial growth factor receptor 1