title: Purification and characterization of a novel NADPH-dependent 2-aminoacetophenone reductase from Arthrobacter sulfureus.
abstract: A novel 2-aminoacetophenone reductase was purified to homogeneity from Arthrobacter sulfureus BW1010. The enzyme is a monomer with a molecular weight of approximately 60 kDa. Using NADPH as coenzyme, it catalyzes the reduction of ketones, especially amine phenyl ketones, and stereospecifically reduces 2-aminoacetophenone to (S)-2-amino-1-phenylethanol (e.e > 99.8%) with the optimal pH at 7.5.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China; Department of Internal Medicine, University of Kentucky School of Medicine, Lexington, KY 40536, USA.
authors.forename: Guogang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Wanru
authors.initials: W
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China. Electronic address: wangjj@im.ac.cn.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000098:Acetophenones / Q000378:metabolism*; D001173:Arthrobacter / Q000201:enzymology*; D001592:Benzyl Alcohols / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000098:Acetophenones; D001592:Benzyl Alcohols; D007659:Ketones; C523155:2-amino-1-phenylethanol; D009249:NADP; C055495:2-aminoacetophenone; D010088:Oxidoreductases
keywords: (S)-2-Amino-1-phenylethanol; 2-Aminoacetophenone reductase; Arthrobacter sulfureus; NADPH-dependent; Reduction