title: Gene expression profiles responses to aphid feeding in chrysanthemum (Chrysanthemum morifolium).
abstract: BACKGROUND
  Chrysanthemum is an important ornamental plant all over the world. It is easily attacked by aphid, Macrosiphoniella sanbourni. The molecular mechanisms of plant defense responses to aphid are only partially understood. Here, we investigate the gene expression changes in response to aphid feeding in chrysanthemum leaf by RNA-Seq technology.
  
  
  RESULTS
  Three libraries were generated from pooled leaf tissues of Chrysanthemum morifolium 'nannongxunzhang' that were collected at different time points with (Y) or without (CK) aphid infestations and mock puncture treatment (Z), and sequenced using an Illumina HiSeqTM 2000 platform. A total of 7,363,292, 7,215,860 and 7,319,841 clean reads were obtained in library CK, Y and Z, respectively. The proportion of clean reads was >97.29% in each library. Approximately 76.35% of the clean reads were mapped to a reference gene database including all known chrysanthemum unigene sequences. 1,157, 527 and 340 differentially expressed genes (DEGs) were identified in the comparison of CK-VS-Y, CK-VS-Z and Z-VS-Y, respectively. These DEGs were involved in phytohormone signaling, cell wall biosynthesis, photosynthesis, reactive oxygen species (ROS) pathway and transcription factor regulatory networks, and so on.
  
  
  CONCLUSIONS
  Changes in gene expression induced by aphid feeding are shown to be multifaceted. There are various forms of crosstalk between different pathways those genes belonging to, which would allow plants to fine-tune its defense responses.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Xia
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Shao
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.forename: Weimin
authors.initials: W
authors.lastname: Fang
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guan
authors.affiliation: College of Horticulture, Nanjing Agricultural University, No,1 Weigang, Nanjing 210095, China. chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001042:Aphids / Q000472:pathogenicity*; D001483:Base Sequence; D031202:Chrysanthemum / Q000235:genetics* / Q000469:parasitology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology; D017422:Sequence Analysis, DNA; D059467:Transcriptome / Q000235:genetics*