title: Direct and indirect consequences on gene expression of a thyroid hormone receptor alpha 1 mutation restricted to Sertoli cells.
abstract: Thyroid hormone is required for the timely transition of Sertoli cells from proliferative to differentiating and maturing. This transition takes place during a critical developmental period in mammals, which in mice is the first post-natal week. In order to identify the underlying molecular mechanisms of this differentiation process, we used Cre/loxP technology to selectively block the function of the thyroid hormone receptor TRα1 in Sertoli cells. We then used RNA-seq to analyze the changes in gene expression induced in the post-natal testis. This differential analysis provides genetic clues to the initial testicular defects resulting from disrupted thyroid hormone signaling, and suggests that Sertoli cells influence germ cells soon after their birth.
authors.affiliation: Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Université Lyon 1, INRA, CNRS, Cedex, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chatonnet
authors.forename: Gabriel
authors.initials: G
authors.lastname: Livera
authors.forename: Betty
authors.initials: B
authors.lastname: Fumel
authors.forename: Sophie
authors.initials: S
authors.lastname: FouchÉCourt
authors.forename: Frédéric
authors.initials: F
authors.lastname: Flamant
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000235:genetics*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D009154:Mutation / Q000235:genetics; D017423:Sequence Analysis, RNA; D012708:Sertoli Cells / Q000378:metabolism*; D037021:Thyroid Hormone Receptors alpha / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D037021:Thyroid Hormone Receptors alpha