title: Osteogenic differentiation of stem cells from human exfoliated deciduous teeth on poly(ε-caprolactone) nanofibers containing strontium phosphate.
abstract: Mimicking the architecture of the extracellular matrix is an effective strategy for tissue engineering. Composite nanofibers similar to natural bone structure can be prepared via an electrospinning technique and used in biomedical applications. Stem cells from human exfoliated deciduous teeth (SHEDs) can differentiate into multiple cell lineages, such as cells that are alternative sources of stem cells for tissue engineering. Strontium has important functions in bone remodeling; for example, this element can simulate bone formation and decrease bone resorption. Incorporating strontium phosphate into nanofibers provides a potential material for bone tissue engineering. This study investigated the potential of poly(ε-caprolactone) (PCL) nanofibers coated or blended with strontium phosphate for the osteogenic differentiation of SHEDs. Cellular morphology and MTT assay revealed that nanofibers effectively support cellular attachment, spreading, and proliferation. Strontium-loaded PCL nanofibers exhibited higher expressions of collagen type I, alkaline phosphatase, biomineralization, and bone-related genes than pure PCL nanofibers during the osteogenic differentiation of SHEDs. This study demonstrated that strontium can be an effective inducer of osteogenesis for SHEDs. Understanding the function of bioceramics (such as strontium) is useful in designing and developing strategies for bone tissue engineering.
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan. Electronic address: f10549@ntut.edu.tw.
authors.forename: Wen-Ta
authors.initials: WT
authors.lastname: Su
authors.affiliation: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
authors.forename: Pai-Shuen
authors.initials: PS
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Surgery, Mackay Memorial Hospital, Taipei, Taiwan.
authors.forename: Te-Yang
authors.initials: TY
authors.lastname: Huang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation; D017931:DNA Primers; D006801:Humans; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D010012:Osteogenesis; D010710:Phosphates; D011091:Polyesters; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013052:Spectrometry, X-Ray Emission; D013234:Stem Cells / Q000166:cytology*; D013324:Strontium; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D017931:DNA Primers; D010710:Phosphates; D011091:Polyesters; C016240:polycaprolactone; C052774:strontium phosphate; D000469:Alkaline Phosphatase; D002118:Calcium; D013324:Strontium
keywords: Osteogenic differentiation; Poly(ε-caprolactone) nanofiber; Stem cells from human exfoliated deciduous teeth; Strontium