title: Evidence of rapid coaggregation of globular proteins during amyloid formation.
abstract: The question of how an aggregating protein can influence aggregation of other proteins located in its vicinity is particularly significant because many proteins coexist in cells. We demonstrate in vitro coaggregation and cross-seeding of lysozyme, bovine serum albumin, insulin, and cytochrome c during their amyloid formation. The coaggregation process seems to be more dependent on the temperature-induced intermediate species of these proteins and less dependent on their sequence identities. Because amyloid-linked inclusions and plaques are recognized as multicomponent entities originating from aggregation of the associated protein, these findings may add new insights into the mechanistic understanding of amyloid-related pathologies.
authors.affiliation: Center for Biologically Inspired System Science, Indian Institute of Technology Jodhpur , Old Residency Road, Jodhpur, Rajasthan, India 342011.
authors.forename: Kriti
authors.initials: K
authors.lastname: Dubey
authors.forename: Bibin G
authors.initials: BG
authors.lastname: Anand
authors.forename: Mayur K
authors.initials: MK
authors.lastname: Temgire
authors.forename: Karunakar
authors.initials: K
authors.lastname: Kar
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000096:biosynthesis* / Q000737:chemistry* / Q000648:ultrastructure; D000686:Amyloidosis / Q000209:etiology / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009113:Muramidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D066329:Protein Aggregates; D066263:Protein Aggregation, Pathological / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence
chemical_list: D000682:Amyloid; D007328:Insulin; D066329:Protein Aggregates; D012710:Serum Albumin, Bovine; D045304:Cytochromes c; D009113:Muramidase