title: Repeated dose 28-day oral toxicity study of moniliformin in rats.
abstract: Moniliformin is a Fusarium mycotoxin mainly produced by several species infecting grains in different climatic conditions. According to our previous studies, it is acutely toxic to rats, with an LD50 cut-off value of 25mg/kg b.w. To further assess the possible health risks of low dose exposure to moniliformin, a subacute oral toxicity study was conducted in Sprague-Dawley rats, adapting OECD guideline 407. Five dose groups and two satellite groups, each consisting of five male rats, were daily exposed to moniliformin by gavage. Two rats in the highest dose group, showed decreased activity followed by acute heart failure and death. The rats of the lower doses (<9mg/kg b.w.) showed no signs of toxicity. The daily intake of moniliformin strongly reduced the phagocytic activity of neutrophils in all dose groups. The decrease continued in the satellite group during the follow-up period, indicating a severe impact on the immune system and a LOAEL value of 3mg/kg b.w. for moniliformin. Moniliformin was rapidly excreted into urine, ranging between 20.2 and 31.5% daily and showed no signs of accumulation. The concentration of moniliformin in faeces was less than 2%, which suggests efficient absorption from the gastrointestinal tract.
authors.affiliation: Chemistry and Toxicology Research Unit, Finnish Food Safety Authority (Evira), Mustialankatu 3, Helsinki FI-00790, Finland. Electronic address: martina.jonsson@evira.fi.
authors.forename: Martina
authors.initials: M
authors.lastname: Jonsson
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Turku, Turku FI-20014, Finland.
authors.forename: Janne
authors.initials: J
authors.lastname: Atosuo
authors.affiliation: Product Safety Unit, Finnish Food Safety Authority (Evira), Helsinki FI-00790, Finland.
authors.forename: Marika
authors.initials: M
authors.lastname: Jestoi
authors.affiliation: Chemistry and Toxicology Research Unit, Finnish Food Safety Authority (Evira), Mustialankatu 3, Helsinki FI-00790, Finland.
authors.forename: Alexis V
authors.initials: AV
authors.lastname: Nathanail
authors.affiliation: Veterinary Virology Research Unit, Finnish Food Safety Authority (Evira), Helsinki FI-00790, Finland.
authors.forename: Ulla-Maija
authors.initials: UM
authors.lastname: Kokkonen
authors.affiliation: Pathology Research Unit, Finnish Food Safety Authority (Evira), Helsinki FI-00790, Finland.
authors.forename: Marjukka
authors.initials: M
authors.lastname: Anttila
authors.affiliation: Chemistry and Toxicology Research Unit, Finnish Food Safety Authority (Evira), Mustialankatu 3, Helsinki FI-00790, Finland.
authors.forename: Pertti
authors.initials: P
authors.lastname: Koivisto
authors.affiliation: Department of Biochemistry and Food Chemistry, University of Turku, Turku FI-20014, Finland.
authors.forename: Esa-Matti
authors.initials: EM
authors.lastname: Lilius
authors.affiliation: Chemistry and Toxicology Research Unit, Finnish Food Safety Authority (Evira), Mustialankatu 3, Helsinki FI-00790, Finland.
authors.forename: Kimmo
authors.initials: K
authors.lastname: Peltonen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003503:Cyclobutanes / Q000633:toxicity* / Q000652:urine; D004305:Dose-Response Relationship, Drug; D005243:Feces / Q000382:microbiology; D005670:Fusarium / Q000737:chemistry; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D006333:Heart Failure / Q000139:chemically induced / Q000473:pathology; D007113:Immunity, Innate / Q000187:drug effects; D007928:Lethal Dose 50; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D059551:Toxicity Tests, Subacute
chemical_list: D003503:Cyclobutanes; C008539:moniliformin
keywords: Excretion; Fusarium mycotoxins; Moniliformin; Sprague-Dawley rats; Subacute toxicity