title: Desulfovibrio desulfuricans isolates from the gut of a single individual: structural and biological lipid A characterization.
abstract: The levels of sulfate-reducing bacteria (SRB), including Desulfovibrionaceae, in the gut increase following a fat-enriched diet. Endotoxins from gut microbiota contribute to the inflammation process, leading to metabolic diseases. Thus, we sought to characterize the lipid A structures of Desulfovibrionaceae lipopolysaccharides (LPS) that are associated with the microbiota inflammatory properties. LPS variants were obtained from two SRB isolates from the gut of a single individual. These LPS variants shared similar lipid A moieties with Enterobacterial LPS, but differed from one another with regard to fatty-acid numbers and endotoxic activity. This first complete structural characterization of Desulfovibrio lipid A gives new insights into previously published data on Desulfovibrio lipid A biosynthesis. LPS microdiversity within SRBs illustrates how adaptation can influence pro-inflammatory potential.
authors.affiliation: Equipe «Endotoxins: Structures and Activities», Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS UMR 8621, Orsay, France.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang-Sun
authors.affiliation: Equipe «Endotoxins: Structures and Activities», Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS UMR 8621, Orsay, France.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Augusto
authors.affiliation: Laboratory of Molecular Microbial Ecology and Ecogenomics, Department of Biological Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Equipe «Endotoxins: Structures and Activities», Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS UMR 8621, Orsay, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Caroff
mesh_terms: D002236:Carbohydrate Conformation; D045285:Desulfovibrio desulfuricans / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008050:Lipid A / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity
chemical_list: D008050:Lipid A
keywords: Cytokine; Desulfovibrionaceae; Endotoxin; Inflammation; Lipid A; Mass spectrometry