title: Concise Review: Differentiation of Human Adult Stem Cells Into Hepatocyte-like Cells In vitro.
abstract: Adult stem cells (ASCs) are undifferentiated cells found throughout the body that divide to replenish dying cells and regenerate damaged tissues, which are the powerful sources for cell therapy and tissue engineering. Bone marrow-derived mesenchymal stem cells (BMSCs), adipose tissue-derived mesenchymal stem cells (ADSCs), and peripheral blood monocytes (PBMCs) are the common ASCs, and many studies indicated that ASCs isolated from various adult tissues could be induced to hepatocyte-like cells in vitro. However, the isolation, culture protocols, characterization of ASCs and hepatocyte-like cells are different. This review aims to describe the isolation and culture procedures for ASCs, to summarize the molecular characterization of ASCs, to characterize function of hepatocyte-like cells, and to discuss the future role of ASCs in cell therapy and tissue engineering.
authors.affiliation: Department of Gastroenterology, Institute of Geriatrics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Yunwei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Institute of Geriatrics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Transfusion Blood Department, Chinese PLA General Hospital, Beijing, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Institute of Geriatrics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Changhao
authors.initials: C
authors.lastname: Cai
authors.affiliation: Department of Gastroenterology, Institute of Geriatrics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yan
keywords: Adipose tissue-derived mesenchymal stem cells Peripheral blood monocytes; Adult stem cells; Bone marrow-derived mesenchymal stem cells; Hepatocyte-like cells