title: Chemical sympathectomy restores baroreceptor-heart rate reflex and heart rate variability in rats with chronic nitric oxide deficiency.
abstract: Nitric oxide (NO) plays a crucial role not only in regulation of blood pressure but also in maintenance of cardiac autonomic tone and its deficiency induced hypertension is accompanied by cardiac autonomic dysfunction. However, underlying mechanisms are not clearly defined. We hypothesized that sympathetic activation mediates hemodynamic and cardiac autonomic changes consequent to deficient NO synthesis. We used chemical sympathectomy by 6-hydroxydopamine to examine the influence of sympathetic innervation on baroreflex sensitivity (BRS) and heart rate variability (HRV) of chronic N(G)-nitro-L-arginine methyl ester (L-NAME) treated adult Wistar rats. BRS was determined from heart rate responses to changes in systolic arterial pressure achieved by intravenous administration of phenylephrine and sodium nitroprusside. Time and frequency domain measures of HRV were calculated from 5-min electrocardiogram recordings. Chronic L-NAME administration (50 mg/kg per day for 7 days orally through gavage) in control rats produced significant elevation of blood pressure, tachycardia, attenuation of BRS for bradycardia and tachycardia reflex and fall in time as well as frequency domain parameters of HRV. Sympathectomy completely abolished the pressor as well as tachycardic effect of chronic L-NAME. In addition, BRS and HRV improved after removal of sympathetic influence in chronic L-NAME treated rats. These results support the concept that an exaggerated sympathetic activity is the principal mechanism of chronic L-NAME hypertension and associated autonomic dysfunction.
authors.affiliation: Department of Physiology, Vardhaman Mahavir Medical College & Safdarjung Hospital, New Delhi, India, Department of Physiology, Hamdard Institute of Medical Sciences & Research, Jamia Hamdard, New Delhi, India. vpciphysiology@yahoo.com.
authors.forename: M
authors.initials: M
authors.lastname: Chaswal
authors.forename: S
authors.initials: S
authors.lastname: Das
authors.forename: J
authors.initials: J
authors.lastname: Prasad
authors.forename: A
authors.initials: A
authors.lastname: Katyal
authors.forename: M
authors.initials: M
authors.lastname: Fahim
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002908:Chronic Disease; D006339:Heart Rate; D008297:Male; D009569:Nitric Oxide / Q000172:deficiency* / Q000378:metabolism*; D016627:Oxidopamine; D011311:Pressoreceptors / Q000187:drug effects / Q000503:physiopathology*; D051381:Rats; D013563:Sympathectomy, Chemical / Q000379:methods*; D016896:Treatment Outcome
chemical_list: D009569:Nitric Oxide; D016627:Oxidopamine