title: [Genome-wide research on fluid shear stress-sensitive signaling pathways in MC3T3-E1 cells].
abstract: OBJECTIVE
  To explore the differentially expressed genes and related signaling pathways in MC3T3-E1 osteo- blasts in response to suitable fluid shear stress values and action time with cDNA microarrays.
  
  
  METHODS
  MC3T3-E1 cells cultured on a cover slip were subjected to fluid shear stress using a parallel plate flow chamber. The harvested RNA was used for microarray hybridization comprising approximately 44 170 genes, as well as for the subsequent real-time quantitative polymerase chain reaction validation of expression levels for selected genes. Microarray results were analyzed by using both GO and Pathway analysis.
  
  
  RESULTS
  Microarray analysis indicated that 884 differentially expressed genes were found. Among these genes, 444 were upregulated, whereas 440 were downregulated. The Notch signal and RIG- I -like receptor signaling pathways were involved in the Pathway analysis. GO analysis mainly involved different functional classifications, such as prostaglandin biosynthesis, nitric oxide-mediated signal transduction, calcium mediated signal, and cellular immune response, among others.
  
  
  CONCLUSIONS
  The mechanism underlying the protective effect of fluid shear stress on MC3T3-E1 cells might be related to promoting cell survival- and inhibiting cell apoptosis-related signaling pathways and biological processes.
authors.forename: Shang
authors.initials: S
authors.lastname: Zhenzhen
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.forename: Sun
authors.initials: S
authors.lastname: Huiqiang
authors.forename: Jia
authors.initials: J
authors.lastname: Keli
mesh_terms: D017209:Apoptosis; D002118:Calcium; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts; D015398:Signal Transduction; D013314:Stress, Mechanical
chemical_list: D002118:Calcium