title: Identification and characterization of nine atypical Candida dubliniensis clinical isolates.
abstract: Candida dubliniensis is a pathogenic yeast of the genus Candida closely related to Candida albicans. The phenotypic similarity of these two species often leads to misidentification of C. dubliniensis isolates in clinical samples. DNA-based methods continue to be the most effective means of discriminating accurately between the two species. Here, we report on the identification of nine unusual Candida isolates that showed ambiguous identification patterns on the basis of their phenotypic and immunological traits. The isolates were categorized into two groups. Group I isolates were unable to produce germ tubes and chlamydospores, and to agglutinate commercial latex particles coated with a mAb highly specific for C. dubliniensis. Group II isolates grew as pink and white colonies on CHROMagar Candida and ChromID Candida, respectively. Carbohydrate assimilation profiles obtained with API/ID32C together with PCR amplification with specific primers and DNA sequencing allowed reliable identification of the nine unusual clinical isolates as C. dubliniensis.
authors.affiliation: Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, University of the Basque Country UPV/EHU, Bilbao, Spain.
authors.forename: Olatz
authors.initials: O
authors.lastname: Albaina
authors.affiliation: Department of Microbiology, Faculty of Medicine, Hawler Medical University, Hawler, Kurdistan, Iraq.|Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, University of the Basque Country UPV/EHU, Bilbao, Spain.
authors.forename: Ismail H
authors.initials: IH
authors.lastname: Sahand
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Odontología, Universidad de Buenos Aires, Argentina.
authors.forename: María I
authors.initials: MI
authors.lastname: Brusca
authors.affiliation: Microbiology Research Laboratory, Division of Oral Biosciences, School of Dental Science and Dublin Dental University Hospital, Trinity College, Dublin 2, Ireland.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Sullivan
authors.affiliation: Departamento de Química Aplicada, Facultad de Ciencias Químicas, University of the Basque Country UPV/EHU, Donostia-San Sebastián, Spain.
authors.forename: Iñigo
authors.initials: I
authors.lastname: Fernández de Larrinoa
authors.affiliation: Departamento de Enfermería I, Escuela Universitaria de Enfermería, University of the Basque Country UPV/EHU, Bilbao, Spain.|Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, University of the Basque Country UPV/EHU, Bilbao, Spain.
authors.forename: María D
authors.initials: MD
authors.lastname: Moragues
mesh_terms: D000908:Antibodies, Fungal / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D002177:Candidiasis / Q000382:microbiology*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007841:Latex Fixation Tests; D008969:Molecular Sequence Data; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D004271:DNA, Fungal