title: Routes to and from the plasma membrane: bulk flow versus signal mediated endocytosis.
abstract: Transport of proteins via the secretory pathway is controlled by a combination of signal dependent cargo selection as well as unspecific bulk flow of membranes and aqueous lumen. Using the plant vacuolar sorting receptor as model for membrane spanning proteins, we have distinguished bulk flow from signal mediated protein targeting in biosynthetic and endocytic transport routes and investigated the influence of transmembrane domain length. More specifically, long transmembrane domains seem to prevent ER retention, either by stimulating export or preventing recycling from post ER compartments. Long transmembrane domains also seem to prevent endocytic bulk flow from the plasma membrane, but the presence of specific endocytosis signals overrules this in a dominant manner.
authors.affiliation: a Centre for Plant Sciences ; University of Leeds ; Leeds , United Kingdom.
authors.forename: David C
authors.initials: DC
authors.lastname: Gershlick
authors.forename: Carine De Marcos
authors.initials: Cde M
authors.lastname: Lousa
authors.forename: Lucy
authors.initials: L
authors.lastname: Farmer
authors.forename: Jurgen
authors.initials: J
authors.lastname: Denecke
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism; D021381:Protein Transport; D017384:Sequence Deletion; D015398:Signal Transduction; D014026:Nicotiana / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D050505:Mutant Proteins
keywords: bulk-flow; endocytosis; protein trafficking; sorting motifs; vacuolar sorting receptor