title: Establishment of an efficient and rapid method of multiple shoot regeneration and a comparative phenolics profile in in vitro and greenhouse-grown plants of Psophocarpus tetragonolobus (L.) DC.
abstract: An in vitro method of multiple shoot induction and plant regeneration in Psophocarpus tetragonolobus (L.) DC was developed. Cotyledons, hypocotyls, epicotyls, internodal and young seedling leaves were used as explants. MS media supplemented with various concentrations of either thidiazuron (TDZ) or N6-benzylaminopurine (BAP) along with NAA or IAA combinations were used to determine their influence on multiple shoot induction. MS media supplemented with TDZ induced direct shoot regeneration when epicotyls and internodal segments were used as explants. TDZ at 3 mg L(-1) induced highest rate (89.2 ± 3.28%) of regeneration with (13.4 ± 2.04) shoots per explant. MS media supplemented with BAP in combination with NAA or IAA induced callus mediated regeneration when cotyledons and hypocotyls were used as explants. BAP (2.5 mg L(-1)) and IAA (0.2 mg L(-1)) induced highest rate (100 ± 2.66%) of regeneration with (23.2 ± 2.66) shoots per explant. Mature plants produced from regenerated shoots were transferred successfully to the greenhouse. In a comparative study, the phenolics contents of various parts of greenhouse-grown plants with that of in vitro-raised plants showed significant variations.
authors.affiliation: a Plant Molecular Biology and Genetic Engineering Division ; National Botanical Research Institute ; Rana Pratap Marg ; Lucknow , Uttar Pradesh , India.
authors.forename: Vinayak
authors.initials: V
authors.lastname: Singh
authors.forename: Namita Singh
authors.initials: NS
authors.lastname: Chauhan
authors.forename: Mohit
authors.initials: M
authors.lastname: Singh
authors.forename: Asif
authors.initials: A
authors.lastname: Idris
authors.forename: Raju
authors.initials: R
authors.lastname: Madanala
authors.forename: Veena
authors.initials: V
authors.lastname: Pande
authors.forename: Chandra Sekhar
authors.initials: CS
authors.lastname: Mohanty
mesh_terms: D001593:Benzyl Compounds / Q000494:pharmacology; D018548:Cotyledon / Q000187:drug effects / Q000502:physiology; D007887:Fabaceae / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D018546:Hypocotyl / Q000187:drug effects / Q000502:physiology; D007210:Indoleacetic Acids / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010636:Phenols / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology*; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism; D011687:Purines / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects; D064210:Secondary Metabolism / Q000187:drug effects; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D001593:Benzyl Compounds; D005419:Flavonoids; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010636:Phenols; D011687:Purines; C034182:1-naphthaleneacetic acid; C030737:indoleacetic acid; C480551:benzylaminopurine
keywords: BAP; BAP, 6-benzylaminopurine; IAA; IAA, 3-indoleacetic acid; NAA; NAA, naphthalene-acetic acid; TDZ; Winged bean; hypocotyls