title: Multidimensional gas chromatography hyphenated to mass spectrometry and olfactometry for the volatile analysis of citrus hybrid peel extract.
abstract: In this work, the volatile composition of the peel extract of limequat, a natural citrus hybrid, was investigated by using both conventional (gas chromatography-mass spectrometry [GC-MS], deconvolution) and more advanced (multidimensional GC-MS/olfactometry [MDGC-MS/O] and enantioselective [Es]-MDGC-MS/O) analytical techniques. Although the GC-MS analysis identified most of the components, some peaks remained unidentified. These unknown peaks were elucidated by deconvolution processing and using a selectable one-dimensional or two-dimensional GC-MS system equipped with an olfactometry port. The MDGC results, with both polar and chiral second dimensions, hyphenated to mass spectrometry and olfactometry, proved to be particularly useful for the identification and chiral characterization of coeluted trace compounds. In particular, the application of the Es-MDGC-MS/O configurations was used to confirm, by on-line enrichment, the presence of cis-δ-jasminlactone. This technique was fundamental for the reliable identification and exact determination of the enantiomeric distribution of this chiral compound, as well as for the sensorial evaluation of each enantiomer at the sniffing port.
authors.affiliation: Analytical Innovation, Corporate R&D Division, Firmenich SA, Geneva, Switzerland; Universidade Federal do Rio de Janeiro, Instituto de Química, LADETEC, Ilha do Fundão, Rio de Janeiro 21941-909, RJ, Brazil. Electronic address: casilli@iq.ufrj.br.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Casilli
authors.affiliation: Analytical Innovation, Corporate R&D Division, Firmenich SA, Geneva, Switzerland.
authors.forename: Erik
authors.initials: E
authors.lastname: Decorzant
authors.affiliation: Analytical Innovation, Corporate R&D Division, Firmenich SA, Geneva, Switzerland.
authors.forename: Alain
authors.initials: A
authors.lastname: Jaquier
authors.affiliation: Analytical Innovation, Corporate R&D Division, Firmenich SA, Geneva, Switzerland. Electronic address: estelle.delort@firmenich.com.
authors.forename: Estelle
authors.initials: E
authors.lastname: Delort
mesh_terms: D002957:Citrus / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D064367:Olfactometry / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry*; D013237:Stereoisomerism; D014835:Volatilization
chemical_list: D010936:Plant Extracts
keywords: Chirality; Enantio-gas chromatography; Eustis limequat; On-line enrichment; Sniffing port