title: MiR-203 inhibits melanoma invasive and proliferative abilities by targeting the polycomb group gene BMI1.
abstract: Metastasis is the major problem in malignant melanoma, posing a therapeutic challenge to clinicians. The investigation of the underlying mechanism driving this progress remains a large unmet need. In this study, we revealed a miR-203-BMI1 axis that regulated melanoma metastasis. We found significantly deregulation of miR-203 and up-regulation of BMI1 in melanoma, particularly in metastatic melanoma. An inverse correlation between the levels of miR-203 and BMI1 was further observed in melanoma tissues and cell lines. We also identified BMI1 as a downstream target gene of miR-203, which bound to the 3'UTR of BMI1. Overexpression of miR-203 was associated with decreased BMI1 expression and impaired cell invasion and tumor sphere formation activities. Re-expression of BMI1 markedly rescued miR-203-mediated suppression of these events. Taken together, our results demonstrated that miR-203 regulated melanoma invasive and proliferative abilities in part by targeting BMI1, providing new insights into potential mechanisms of melanoma metastasis.
authors.affiliation: Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Medical Oncology, Jinling Hospital, Nanjing 210002, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Dermatology and Venereal Disease, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. Electronic address: zhanghaiping_2000@163.com.
authors.forename: Haiping
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Dermatology and Venereal Disease, Capital Medical University, Beijing 100069, China. Electronic address: lianshi_2020@163.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Lian
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000628:therapy; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C069077:BMI1 protein, human; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: BMI1; Invasion; Melanoma; Metastasis; Proliferation; miR-203