title: Study of microRNA in diabetic nephropathy: isolation, quantification and biological function.
abstract: In recent years, several studies have reported dysregulation of microRNA expression in disease with a growing interest focussed on targeting microRNAs as a novel therapy for human disease. This is especially true in diabetic nephropathy where the expression of several microRNAs is dysregulated, contributing to the increased expression and accumulation of extracellular matrix proteins and increased pro-fibrotic signalling, ultimately resulting in renal fibrosis. The development of various techniques and microRNA reagents has enabled work to progress very rapidly in this area. In the present article, the authors describe the methods they have used that have enabled them to contribute to our current understanding of the role of microRNAs in diabetic nephropathy.
authors.affiliation: JDRF Danielle Alberti Memorial Centre for Diabetes Complications, Diabetes Division, Baker IDI Heart and Diabetes Institute, Melbourne, Victoria, Australia.
authors.forename: Phillip
authors.initials: P
authors.lastname: Kantharidis
authors.forename: Shinji
authors.initials: S
authors.lastname: Hagiwara
authors.forename: Eoin
authors.initials: E
authors.lastname: Brennan
authors.forename: Aaron D
authors.initials: AD
authors.lastname: McClelland
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003928:Diabetic Nephropathies / Q000235:genetics* / Q000378:metabolism / Q000503:physiopathology; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense
keywords: QPCR; diabetic nephropathy; microRNA; reporter assay; transfection