title: Enhancing of sugar cane bagasse hydrolysis by Annulohypoxylon stygium glycohydrolases.
abstract: The aim of this study was to develop a bioprocess for the production of β-glucosidase and pectinase from the fungus Annulohypoxylon stygium DR47. Media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase at pH 4.0 and 10.13 U/mL of β-glucosidase at pH 5.0. In addition, the enzymes extracts were able to replace partially Celluclast 1.5L in sugar cane bagasse hydrolysis. Proteomic analysis from A. stygium cultures revealed accessory enzymes, mainly belong to the families GH3 and GH54, that would support enhancement of commercial cocktail saccharification yields. This is the first report describing bioreactor optimization for enzyme production from A. stygium with a view for more efficient degradation of sugar cane bagasse.
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo (USP), Avenida Lineu Prestes 1374, CEP 05508-900 São Paulo, Brazil; Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil. Electronic address: diogorobl@usp.br.
authors.forename: Diogo
authors.initials: D
authors.lastname: Robl
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Patrícia dos Santos
authors.initials: Pdos S
authors.lastname: Costa
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Büchli
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Deise Juliana da Silva
authors.initials: DJ
authors.lastname: Lima
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Priscila da Silva
authors.initials: Pda S
authors.lastname: Delabona
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: Fabio Marcio
authors.initials: FM
authors.lastname: Squina
authors.affiliation: Department of Basic Pathology, Federal University of Paraná (UFPR), CEP 81531-980 Curitiba, Paraná, Brazil.
authors.forename: Ida Chapaval
authors.initials: IC
authors.lastname: Pimentel
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo (USP), Avenida Lineu Prestes 1374, CEP 05508-900 São Paulo, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Padilla
authors.affiliation: Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Centre of Research in Energy and Materials (CNPEM), Rua Giuseppe Maximo Scolfaro 10000, Pólo II de Alta Tecnologia, CEP 13083-970 Campinas, São Paulo, Brazil.
authors.forename: José Geraldo da Cruz
authors.initials: JG
authors.lastname: Pradella
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D004795:Enzyme Stability / Q000187:drug effects; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D011096:Polygalacturonase / Q000096:biosynthesis; D040901:Proteomics; D031786:Saccharum / Q000737:chemistry*; D013696:Temperature; D001617:beta-Glucosidase / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D002244:Carbon; D002482:Cellulose; C027433:bagasse; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; D001617:beta-Glucosidase
keywords: Annulohypoxylon stygium; Hydrolysis; Pectinase; Sugar cane bagasse; β-Glucosidase