title: Functional analysis of an auxin-inducible DNA-binding protein gene.
abstract: Over the past decades, several studies indicate a correlation between the phytohormone auxin and cell division. The molecular players of this signaling pathway are now being uncovered. DNA Binding Protein1 from Arabidopsis (AtDBP1) is an auxin-inducible gene able to bind DNA non-specifically. In this work the tissue-expression pattern of this gene was investigated. Promoter-GUS analysis demonstrated that the AtDBP1 promoter is active in regions exhibiting intense cell division such as meristems and nematode feeding sites. Also, the promoter expression was modulated upon incubation with cell cycle blockers, indicating a potential role in cell division for this gene. Lastly, AtDBP1 antisense plants presented a higher insensitivity to auxin, and interfered negatively with auxin-induced callus formation and reduced apical dominance.
authors.affiliation: a Laboratório de Genômica Funcional e Transdução de Sinal; Departamento de Genética ; Universidade Federal do Rio de Janeiro ; Brazil.
authors.forename: Any
authors.initials: A
authors.lastname: Bernstein
authors.forename: Amanda
authors.initials: A
authors.lastname: Mangeon
authors.forename: Janice
authors.initials: J
authors.lastname: Almeida-Engler
authors.forename: Gilbert
authors.initials: G
authors.lastname: Engler
authors.forename: Marc
authors.initials: M
authors.lastname: Van Montagu
authors.forename: Gilberto
authors.initials: G
authors.lastname: Sachetto-Martins
authors.forename: Dulce Eleonora
authors.initials: DE
authors.lastname: de Oliveira
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015200:Fluoroimmunoassay; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D018745:Genome, Plant; D015698:Genomic Library; D007210:Indoleacetic Acids / Q000378:metabolism / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011994:Recombinant Proteins
chemical_list: D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D007210:Indoleacetic Acids; D011994:Recombinant Proteins; C000592774:DBP1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases
keywords: AtDBP1, DNA binding protein1 from Arabidopsis thaliana; DNA-binding protein1 (DBP1); auxin; cell cycle; developmental gene expression; nematode