title: Identification and characterization of the antigenic site (epitope) on bovine β-lactoglobulin: common residues in linear and conformational epitopes.
abstract: BACKGROUND
  β-Lactoglobulin is recognised as one of major allergens in milk and its epitopes include linear and conformational epitopes contributed to milk allergy.
  
  
  RESULTS
  In our work, two types of epitopes have been identified. Linear epitopes identified by using SPOT™ peptide arrays approach and three common peptide sequences AA77-82 (KIPAVF), AA126-131 (PEVDNE) and AA142-147 (ALPMHI) were obtained by reacting with specific sera from two rabbits. At the same time, mimotopes were screened by the panning of a phage display peptide library and the corresponding conformational epitopes were calculated by the web tool of Peptiope server with Mapitope algorithm. Three conformational epitopes against two specific sera were identified, in which there were 15 common residues as well and located in the different position and appeared mainly as an α-helix.
  
  
  CONCLUSIONS
  Common residues on the linear and conformational epitopes were identified in the first time, respectively, which could be regarded as informative epitopes for detection of allergen in dairy products.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P.R. China.|School of Life Sciences and Food Engineering, Nanchang University, Nanchang, 330031, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P.R. China.|School of Life Sciences and Food Engineering, Nanchang University, Nanchang, 330031, P.R. China.
authors.forename: Shuilin
authors.initials: S
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P.R. China.|School of Life Sciences and Food Engineering, Nanchang University, Nanchang, 330031, P.R. China.
authors.forename: Shengfa
authors.initials: S
authors.lastname: He
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P.R. China.|School of Life Sciences and Food Engineering, Nanchang University, Nanchang, 330031, P.R. China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P.R. China.|Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang, 330047, P.R. China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Chen
mesh_terms: D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D018604:Epitope Mapping; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D007782:Lactoglobulins / Q000737:chemistry*; D008892:Milk / Q000737:chemistry / Q000276:immunology*; D016269:Milk Hypersensitivity / Q000276:immunology*; D010455:Peptides / Q000032:analysis; D017433:Protein Structure, Secondary; D011817:Rabbits
chemical_list: D000485:Allergens; D000939:Epitopes; D007782:Lactoglobulins; D010455:Peptides
keywords: allergen; bovine β-lactoglobulin; common residues; epitope