title: Clinical systemic lupeol administration for canine oral malignant melanoma.
abstract: Canine oral malignant melanoma (COMM) is the most aggressive malignant tumor in dogs. Lupeol is a triterpene extracted from various fruits and vegetables that reportedly inhibits melanoma cell proliferationin vitroandin vivo. In this study, the efficacy of subcutaneous lupeol for spontaneous COMM was evaluated. A total of 11 dogs (3, 5 and 3 dogs diagnosed with clinical stage I, II and III melanoma, respectively) were evaluated. Subcutaneous lupeol (10 mg/kg) was administered postoperatively at various time points to treat these 11 COMM cases. Of the 11 subjects, 7 exhibited no local recurrence 180 days postoperatively and no severe adverse effects were observed in any of the cases. Furthermore, no distant metastasis was observed during the experimental period. Therefore, systemic lupeol may prevent local tumor progression and distant metastasis and may be a novel adjuvant treatment for the treatment of COMM.
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Inoru
authors.initials: I
authors.lastname: Yokoe
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Azuma
authors.affiliation: Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry and Fisheries Research Center, Akita 010-1623, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Hata
authors.affiliation: Sakamoto Bio Co., Ltd., Yuuwa-Memeki, Akita 010-1233, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Mukaiyama
authors.affiliation: Sakamoto Bio Co., Ltd., Yuuwa-Memeki, Akita 010-1233, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Goto
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsuka
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Imagawa
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Norihiko
authors.initials: N
authors.lastname: Itoh
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Murahata
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Osaki
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Saburo
authors.initials: S
authors.lastname: Minami
authors.affiliation: School of Veterinary Medicine, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
authors.forename: Yoshiharu
authors.initials: Y
authors.lastname: Okamoto
keywords: canine; lupeol; melanoma; survival time; systemic injection