title: A wheat CCAAT box-binding transcription factor increases the grain yield of wheat with less fertilizer input.
abstract: Increasing fertilizer consumption has led to low fertilizer use efficiency and environmental problems. Identifying nutrient-efficient genes will facilitate the breeding of crops with improved fertilizer use efficiency. This research performed a genome-wide sequence analysis of the A (NFYA), B (NFYB), and C (NFYC) subunits of Nuclear Factor Y (NF-Y) in wheat (Triticum aestivum) and further investigated their responses to nitrogen and phosphorus availability in wheat seedlings. Sequence mining together with gene cloning identified 18 NFYAs, 34 NFYBs, and 28 NFYCs. The expression of most NFYAs positively responded to low nitrogen and phosphorus availability. In contrast, microRNA169 negatively responded to low nitrogen and phosphorus availability and degraded NFYAs. Overexpressing TaNFYA-B1, a low-nitrogen- and low-phosphorus-inducible NFYA transcript factor on chromosome 6B, significantly increased both nitrogen and phosphorus uptake and grain yield under differing nitrogen and phosphorus supply levels in a field experiment. The increased nitrogen and phosphorus uptake may have resulted from the fact that that overexpressing TaNFYA-B1 stimulated root development and up-regulated the expression of both nitrate and phosphate transporters in roots. Our results suggest that TaNFYA-B1 plays essential roles in root development and in nitrogen and phosphorus usage in wheat. Furthermore, our results provide new knowledge and valuable gene resources that should be useful in efforts to breed crops targeting high yield with less fertilizer input.
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Baoyuan
authors.initials: B
authors.lastname: Qu
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xue
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Teng
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: An
authors.initials: A
authors.lastname: Shao
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wenying
authors.initials: W
authors.lastname: Ma
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory for Plant Cell and Chromosome Engineering (B.Q., X.H., Y.Z., W.T., A.S., X.Z., W.M., Ju.W., B.L., Z.L., Y.T.) and National Center for Plant Gene Research (Ji.W.), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China yptong@genetics.ac.cn.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Tong
mesh_terms: D000383:Agriculture; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D005308:Fertilizers; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000172:deficiency; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism; D010758:Phosphorus / Q000172:deficiency; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development; D018520:Plant Shoots / Q000235:genetics; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D005308:Fertilizers; D035683:MicroRNAs; D009566:Nitrates; D028061:Phosphate Transport Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D010758:Phosphorus; D009584:Nitrogen