title: Cytogenetic and molecular markers for detecting Aegilops uniaristata chromosomes in a wheat background.
abstract: Aegilops uniaristata has many agronomically useful traits that can be used for wheat breeding. So far, a Triticum turgidum - Ae. uniaristata amphiploid and one set of Chinese Spring (CS) - Ae. uniaristata addition lines have been produced. To guide Ae. uniaristata chromatin transformation from these lines into cultivated wheat through chromosome engineering, reliable cytogenetic and molecular markers specific for Ae. uniaristata chromosomes need to be developed. Standard C-banding shows that C-bands mainly exist in the centromeric regions of Ae. uniaristata but rarely at the distal ends. Fluorescence in situ hybridization (FISH) using (GAA)8 as a probe showed that the hybridization signal of chromosomes 1N-7N are different, thus (GAA)8 can be used to identify all Ae. uniaristata chromosomes in wheat background simultaneously. Moreover, a total of 42 molecular markers specific for Ae. uniaristata chromosomes were developed by screening expressed sequence tag - sequence tagged site (EST-STS), expressed sequence tag - simple sequence repeat (EST-SSR), and PCR-based landmark unique gene (PLUG) primers. The markers were subsequently localized using the CS - Ae. uniaristata addition lines and different wheat cultivars as controls. The cytogenetic and molecular markers developed herein will be helpful for screening and identifying wheat - Ae. uniaristata progeny.
authors.affiliation: a School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Gong
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.forename: Jianping
authors.initials: J
authors.lastname: Zhou
authors.forename: Genying
authors.initials: G
authors.lastname: Li
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.forename: Chengyan
authors.initials: C
authors.lastname: Huang
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhao
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
mesh_terms: D002871:Chromosome Banding; D032461:Chromosomes, Plant; D005819:Genetic Markers; D017404:In Situ Hybridization, Fluorescence; D006109:Poaceae / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: Aegilops uniaristata; C-banding; FISH; coloration des bandes C; marqueur moléculaire; molecular marker