title: Acute and sub-chronic toxicity studies of honokiol microemulsion.
abstract: The purpose of this study was to investigate the acute and sub-chronic toxicity of honokiol microemulsion. In the acute toxicity tests, the mice were intravenously injected graded doses of honokiol microemulsion and were observed for toxic symptoms and mortality daily for 14 days. In the sub-chronic toxicity study, rats were injected honokiol microemulsion at doses of 100, 500, 2500 μg/kg body weight (BW) for 30 days. After 30 days treatment and 14 days recovery, the rats were sacrificed for hematological, biochemical and histological examination. In the acute toxicity tests, the estimated median lethal dosage (LD50) was 50.5mg/kg body weight in mice. In the sub-chronic toxicity tests, the non-toxic reaction dose was 500 μg/kg body weight. In each treatment group, degeneration or/and necrosis in vascular endothelial cells and structure change of vessel wall can be observed in the injection site (cauda vein) of a few animals while there were no changes in the vessels of other organs. The overall findings of this study indicate that the honokiol microemulsion is non-toxic up to 500 μg/kg body weight, and it has irritation to the vascular of the injection site which should be paid attention to in clinical medication.
authors.affiliation: New Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Drug Safety Evaluation Center, China Institute for Radiation Protection, Taiyuan 030006, China; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
authors.affiliation: Drug Safety Evaluation Center, China Institute for Radiation Protection, Taiyuan 030006, China; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Drug Safety Evaluation Center, China Institute for Radiation Protection, Taiyuan 030006, China; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: An
authors.affiliation: Drug Safety Evaluation Center, China Institute for Radiation Protection, Taiyuan 030006, China; Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Taiyuan 030006, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Wen
authors.affiliation: New Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wang
authors.affiliation: New Drug Safety Evaluation Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, China. Electronic address: jinhongtao@imm.ac.cn.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Jin
authors.affiliation: School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. Electronic address: chenshizhong66@163.com.
authors.forename: Shizhong
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001713:Biphenyl Compounds / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004655:Emulsions; D007275:Injections, Intravenous; D007928:Lethal Dose 50; D017705:Lignans / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D008813:Mice, Inbred ICR; D019055:No-Observed-Adverse-Effect Level; D017207:Rats, Sprague-Dawley; D018570:Risk Assessment; D013997:Time Factors; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D004655:Emulsions; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
keywords: Acute toxicity; Honokiol microemulsion; Sub-chronic toxicity