title: Temporal separation of replication and transcription during S-phase progression.
abstract: Transcriptional events during S-phase are critical for cell cycle progression. Here, by using a nascent RNA capture assay coupled with high-throughput sequencing, we determined the temporal patterns of transcriptional events that occur during S-phase. We show that genes involved in critical S-phase-specific biological processes such as nucleosome assembly and DNA repair have temporal transcription patterns across S-phase that are not evident from total RNA levels. By comparing transcription timing with replication timing in S-phase, we show that early replicating genes show increased transcription late in S-phase whereas late replicating genes are predominantly transcribed early in S-phase. Global anti-correlation between replication and transcription timing was observed only based on nascent RNA but not total RNA. Our data provides a detailed view of ongoing transcriptional events during the S-phase of cell cycle, and supports that transcription and replication are temporally separated.
authors.affiliation: a Department of Medical Genetics; Institute of Biomedicine; The Sahlgrenska Academy ; University of Gothenburg ; Gothenburg , Sweden.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Meryet-Figuiere
authors.forename: Babak
authors.initials: B
authors.lastname: Alaei-Mahabadi
authors.forename: Mohamad Moustafa
authors.initials: MM
authors.lastname: Ali
authors.forename: Sanhita
authors.initials: S
authors.lastname: Mitra
authors.forename: Santhilal
authors.initials: S
authors.lastname: Subhash
authors.forename: Gaurav Kumar
authors.initials: GK
authors.lastname: Pandey
authors.forename: Erik
authors.initials: E
authors.lastname: Larsson
authors.forename: Chandrasekhar
authors.initials: C
authors.lastname: Kanduri
mesh_terms: D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D004261:DNA Replication / Q000235:genetics / Q000502:physiology*; D042522:DNA Replication Timing / Q000235:genetics / Q000502:physiology; D006801:Humans; D016196:S Phase / Q000235:genetics
keywords: Cell cycle; Ethynyl Uridine, EtU; Replication; Replication timing; S-phase; Transcription