title: Infusion of dendritic cells carrying donor lymphocytes treated with 8-methoxypsoralen and ultraviolet A light induces CD19+ IL-10+ regulatory B cells and promotes skin allograft survival.
abstract: PUVA-SP DCs are immature dendritic cells (imDCs) that have taken up donor splenic lymphocytes treated with 8-methoxypsoralen and ultraviolet A light (PUVA-SPs). Phagocytosis of donor PUVA-SPs does not stimulate phenotype maturation of recipient imDCs, and infusion of PUVA-SP DCs can induce CD4(+)CD25(high)Foxp3(+) regulatory T cells (Treg). However, the regulatory effect of PUVA-SP DCs on B cells is poorly understood. In this study, we compared PUVA-SP DCs with imDCs in terms of lipopolysaccharide (LPS) responsiveness and flow cytometric analysis of interleukin (IL) 10 expression in splenic CD19(+)B cells. Our results demonstrate that PUVA-SP DCs can suppress subsequent LPS-induced DC maturation and that infusion of PUVA-SP DCs, in the absence of an immunosuppressant, significantly promotes skin allograft survival. This effect was associated with up-regulation of circulating regulatory B cells exhibiting preferential IL-10 secretion. Our results suggest that effective treatments involving infusion of PUVA-SP DCs is likely related to the modulation not only of T-cell and Treg functions but also of B-cell and regulatory B-cell (Breg) functions.
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: D
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Qian
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China.
authors.forename: C
authors.initials: C
authors.lastname: Ming
authors.affiliation: Organ Transplantation Institute, 309th Hospital of the Chinese People's Liberation Army, Beijing, People's Republic of China. Electronic address: shibingyi@medmail.com.cn.
authors.forename: B
authors.initials: B
authors.lastname: Shi
mesh_terms: D064591:Allografts; D000818:Animals; D018941:Antigens, CD19 / Q000276:immunology*; D060151:B-Lymphocytes, Regulatory / Q000276:immunology*; D002454:Cell Differentiation / Q000187:drug effects; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D006085:Graft Survival / Q000187:drug effects / Q000528:radiation effects; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D008730:Methoxsalen / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation; D014466:Ultraviolet Rays
chemical_list: D018941:Antigens, CD19; D016753:Interleukin-10; D008730:Methoxsalen