title: Production of rohitukine in leaves and seeds of Dysoxylum binectariferum: an alternate renewable resource.
abstract: BACKGROUND
  Rohitukine is an important precursor for the synthesis of potential anticancer drugs flavopiridol (Sanofi-Aventis) and P-276-00 (Piramal Healthcare Limited, Mumbai, India). Trunk bark of Dysoxylum binectariferum (Roxb.) Hook. f. ex Bedd. (Meliaceae) is the widely used source for isolation of rohitukine. However, removal of trunk bark threatens the survival of the tree.
  
  
  OBJECTIVE
  To investigate the amount of rohitukine accumulated in other tissues of D. binectariferum.
  
  
  METHODS
  Rohitukine standard was isolated from leaves of D. binectariferum. Its purity was ascertained using HR-MS and NMR. Crude extracts were prepared from different tissues of D. binectariferum. Rohitukine content in all the tissues was quantified by HPLC.
  
  
  RESULTS
  Rohitukine accumulates in a significant amount in seeds, trunk bark, leaves, twigs, and fruits of D. binectariferum. Seeds have the highest rohitukine content (2.42%, dry weight) followed by trunk bark (1.34%, dry weight), leaves (1.064%, dry weight), twigs (0.844% dry weight), and fruits (0.4559% dry weight).
  
  
  CONCLUSIONS
  Seeds and leaves of D. binectariferum could be used as alternate renewable sources for isolation of rohitukine.
authors.affiliation: Indian Institute of Integrative Medicine (CSIR-IIIM), Council of Scientific and Industrial Research , Jammu , India and.
authors.forename: Vidushi
authors.initials: V
authors.lastname: Mahajan
authors.forename: Neha
authors.initials: N
authors.lastname: Sharma
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Vikram
authors.initials: V
authors.lastname: Bhardwaj
authors.forename: Asif
authors.initials: A
authors.lastname: Ali
authors.forename: R K
authors.initials: RK
authors.lastname: Khajuria
authors.forename: Y S
authors.initials: YS
authors.lastname: Bedi
authors.forename: Ram A
authors.initials: RA
authors.lastname: Vishwakarma
authors.forename: Sumit G
authors.initials: SG
authors.lastname: Gandhi
mesh_terms: D002867:Chromones / Q000302:isolation & purification* / Q000378:metabolism; D029582:Meliaceae / Q000378:metabolism; D010880:Piperidines / Q000302:isolation & purification* / Q000378:metabolism; D010936:Plant Extracts / Q000302:isolation & purification* / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism
chemical_list: C573233:5,7-dihydroxy-2-methyl-8-(4-(3-hydroxy-1-methyl)-piperidinyl)-4H-1-benzopyran-4-one; D002867:Chromones; D010880:Piperidines; D010936:Plant Extracts
keywords: Amoora rohituka; DNA barcoding; flavopiridol