title: Platelet rich plasma enhances the immunohistochemical expression of platelet derived growth factor and vascular endothelial growth factor in the synovium of the meniscectomized rat models of osteoarthritis.
abstract: This study was carried out on a rat model of surgically-induced osteoarthritis (OA) to assess the histological and immunohistochemical changes in the synovial membrane and to evaluate the effects of intra-articular injection of platelet rich plasma (PRP) in such cases. Forty five male albino rats were divided into 3 equal groups; control, surgically-induced OA and surgically-induced OA followed by intra-articular injection of PRP. Knee joints were processed for histological and immunohistochemical staining with anti-platelet derived growth factor (PDGF-A) and anti-vascular endothelial growth factor (VEGF) and the area percentages of immunostaining were measured by digital image analysis. Serum levels of PDGF-A and VEGF were analyzed by ELISA. The osteoarthritis research society international (OARSI) score was significantly higher in OA (2433.8±254) than in control (230.4±37.8; p<0.001) and in PRP-treated tissues (759.7±45.8; p<0.001). The immunostained area percentages for PDGF-A was significantly higher in PRP-treated tissues (20.6±2.4) than in OA (11.06±1.3; p=0.007) and in control tissues (4.1±0.78; p<0.001). Likewise, the immunostained area percentage for VEGF was significantly higher in PRP-treated tissues (22.5±1.6) than in OA (14.9±1; p=0. 002) and in control tissues (6.5±0.7; p<0.001). ELISA analysis revealed a significant increase in serum levels of the PDGF-A and VEGF after intraarticular PRP injection when compared to the other groups (p<0.000). The present study concluded that intra-articular injection of PRP could produce optimizing effects in surgically induced OA in the form of; decreasing the OARSI score, improving the inflammatory events in synovium and modulating the PDGF-A and VEGF serum levels and synovial tissue immunoexpression. These effects could be reflected positively on the associated chondral defect.
authors.affiliation: Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Anatomy and Embryology, Almansoura University, Almansoura, Egypt. Electronic address: shaima.masry@yahoo.com.
authors.forename: Shaima M
authors.initials: SM
authors.lastname: Almasry
authors.affiliation: Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Histology and Cell Biology, Zagazig University, Zagazig, Egypt.
authors.forename: Hala M
authors.initials: HM
authors.lastname: Soliman
authors.affiliation: Department of Biochemistry and Molecular Medicine, Taibah University, Almadinah Almonawarah, Saudi Arabia; Department of Medical Biochemistry, Zagazig University, Zagazig, Egypt.
authors.forename: Shereen A
authors.initials: SA
authors.lastname: El-Tarhouny
authors.affiliation: Department of Anatomy, Taibah University, Almadinah Almonawarah, Saudi Arabia.
authors.forename: Sami A
authors.initials: SA
authors.lastname: Algaidi
authors.affiliation: Department of Orthopedic Surgery, Al Azhar University, Assuit Branch, Cairo, Egypt.
authors.forename: Ehab M
authors.initials: EM
authors.lastname: Ragab
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D007270:Injections, Intra-Articular; D007719:Knee Joint; D008297:Male; D010003:Osteoarthritis / Q000628:therapy*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D053657:Platelet-Rich Plasma / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013582:Synovial Fluid; D013583:Synovial Membrane / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A
keywords: Osteoarthritis; PDGF; PRP; Rat model; Synovium; VEGF