title: Significance of IL-1RA Polymorphism in Iranian Patients with Inflammatory Bowel Disease.
abstract: OBJECTIVE
  Interleukin (IL)-1 family members play an important role in the pathogenesis of inflammatory bowel disease (IBD). There are conflicting results regarding the association of IL-1 gene cluster single nucleotide polymorphisms (SNPs) with IBD and its clinical features. The aim of this study was to examine IL-1α -889 C/T, IL-1β -511 C/T, IL-1β +3962 C/T, IL-1R Pst-I1970 C/T, and IL-1RA Mspa-I11100 C/T SNPs in Iranian patients.
  
  
  METHODS
  In this study, SNPs of IL-1 family members were investigated in 75 patients with IBD (40 CD and 35 UC), using polymerase chain reaction with sequence-specific primers method.
  
  
  RESULTS
  IL-1β -511 CC genotype was significantly less present in UC compared to controls, while IL-1RA Mspa-I11100 CC was significantly associated with both Crohn's disease (CD) and ulcerative colitis (UC). IL-1α -889 TT genotype was more frequently associated with extraintestinal manifestations. A significant association was observed between IL-1β +3962 TT genotype and the disease activity in IBD. IL-1RA Mspa-I11100 CC was significantly less frequent in CD patients who need immunosuppressive therapy. IL-1RA Mspa-I11100 CT was associated with earlier age of onset in IBD, while TT genotype was associated with higher age of onset in IBD.
  
  
  CONCLUSIONS
  IL-1 SNPs seem to be associated with IBD and could affect the disease severity as well.
authors.affiliation: Department of Gastroenterology and Hepatology, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nasser Ebrahimi
authors.initials: NE
authors.lastname: Daryani
authors.forename: Maryam
authors.initials: M
authors.lastname: Sadr
authors.forename: Shirin
authors.initials: S
authors.lastname: Moossavi
authors.forename: Sepideh
authors.initials: S
authors.lastname: Shahkarami
authors.forename: Samaneh
authors.initials: S
authors.lastname: Soltani
authors.forename: Elham
authors.initials: E
authors.lastname: Farhadi
authors.forename: Nima
authors.initials: N
authors.lastname: Rezaei
mesh_terms: D000328:Adult; D017668:Age of Onset; D003093:Colitis, Ulcerative / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D003424:Crohn Disease / Q000175:diagnosis / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics*; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics; D007492:Iran / Q000453:epidemiology; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012720:Severity of Illness Index
chemical_list: C508584:IL1A protein, human; C508585:IL1B protein, human; C507763:IL1RN protein, human; D007166:Immunosuppressive Agents; D053590:Interleukin 1 Receptor Antagonist Protein; D053582:Interleukin-1alpha; D053583:Interleukin-1beta