title: Biomimetic receptors for bioanalyte detection by quartz crystal microbalances - from molecules to cells.
abstract: A universal label-free detection of bioanalytes can be performed with biomimetic quartz crystal microbalance (QCM) coatings prepared by imprinting strategies. Bulk imprinting was used to detect the endocrine disrupting chemicals (EDCs) known as estradiols. The estrogen 17β-estradiol is one of the most potent EDCs, even at very low concentrations. A highly sensitive, selective and robust QCM sensor was fabricated for real time monitoring of 17β-estradiol in water samples by using molecular imprinted polyurethane. Optimization of porogen (pyrene) and cross-linker (phloroglucinol) levels leads to improved sensitivity, selectivity and response time of the estradiol sensor. Surface imprinting of polyurethane as sensor coating also allowed us to generate interaction sites for the selective recognition of bacteria, even in a very complex mixture of interfering compounds, while they were growing from their spores in nutrient solution. A double molecular imprinting approach was followed to transfer the geometrical features of natural bacteria onto the synthetic polymer to generate biomimetic bacteria. The use of biomimetic bacteria as template makes it possible to prepare multiple sensor coatings with similar sensitivity and selectivity. Thus, cell typing, e.g., differentiation of bacteria strains, bacteria growth profile and extent of their nutrition, can be monitored by biomimetic mass sensors. Obviously, this leads to controlled cell growth in bioreactors.
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Waehringer Strasse 38, Vienna A-1090, Austria. usmanlatif@ciit.net.pk.
authors.forename: Usman
authors.initials: U
authors.lastname: Latif
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Waehringer Strasse 38, Vienna A-1090, Austria. Jianjin.qian@univie.ac.at.
authors.forename: Jianjin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Waehringer Strasse 38, Vienna A-1090, Austria. serpil.can@univie.ac.at.
authors.forename: Serpil
authors.initials: S
authors.lastname: Can
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Waehringer Strasse 38, Vienna A-1090, Austria. Franz.Dickert@univie.ac.at.
authors.forename: Franz L
authors.initials: FL
authors.lastname: Dickert
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification*; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D040761:Biomimetic Materials / Q000138:chemical synthesis*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004958:Estradiol / Q000032:analysis*; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation*; D054802:Molecular Imprinting / Q000379:methods
chemical_list: D001672:Biocompatible Materials; D004958:Estradiol