title: Protective effects of sildenafil citrate administration on cisplatin-induced ovarian damage in rats.
abstract: The aim of this study is to evaluate the effects of sildenafil citrate on cisplatin-induced ovarian toxicity. Thirty-two female rats were divided into four groups. Group 1: saline control; group 2: cisplatin; group 3: sildenafil citrate; and group 4: cisplatin plus sildenafil citrate group. In groups 2 and 4, the rats were injected with 5 mg/kg cisplatin intraperitoneally (i.p.). In groups 3 and 4, the rats were injected with 1.4 mg/kg sildenafil citrate i.p. The ovaries were removed two weeks later in all groups. Histopathologic examination, follicle counting and classification were performed. The expression of anti-Müllerian hormone (AMH) was detected immunohistochemically in the ovarian tissues. Sildenafil alleviated cisplatin-induced histopathological changes in the ovarian tissue. Primordial, secondary and tertiary follicles were diminished in group 2 compared with group 1 (p < 0.05). Pretreatment with sildenafil citrate preserved primordial follicle count in group 4 compared with group 2, and the difference was statistically significant (p < 0.05). According to our results, immunoreactivity intensity of AMH was lower in group 2 compared with group 1 (92.4 ± 3.97 versus 88.8 ± 1.77) but not significantly, whereas immunoreactivity intensity of AMH was higher in group 4 compared with group 2 (88.8 ± 1.77 versus 94.1 ± 2.36; p < 0.05). Our results demonstrated that pretreatment with sildenafil citrate is beneficial for protecting the ovaries from cisplatin-induced damage. Sildenafil citrate can be a choice for fertility preservation.
authors.affiliation: Department of Obstetrics and Gynecology, Balıkesir University School of Medicine , Balikesir , Turkey and.
authors.forename: Mine Islimye
authors.initials: MI
authors.lastname: Taskin
authors.forename: Arzu
authors.initials: A
authors.lastname: Yay
authors.forename: Ertan
authors.initials: E
authors.lastname: Adali
authors.forename: Esra
authors.initials: E
authors.lastname: Balcioglu
authors.forename: Umit
authors.initials: U
authors.lastname: Inceboz
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone / Q000378:metabolism; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry; D015415:Biomarkers / Q000378:metabolism; D002945:Cisplatin / Q000008:administration & dosage / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D005260:Female; D007150:Immunohistochemistry; D007247:Infertility, Female / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007274:Injections, Intraperitoneal; D009866:Oogenesis / Q000187:drug effects; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D058986:Phosphodiesterase 5 Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar; D000068677:Sildenafil Citrate / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D000970:Antineoplastic Agents; D015415:Biomarkers; D058986:Phosphodiesterase 5 Inhibitors; D020011:Protective Agents; D054304:Anti-Mullerian Hormone; D000068677:Sildenafil Citrate; D002945:Cisplatin
keywords: AMH; cisplatin; primordial follicle; sildenafil citrate