title: Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) redox function negatively regulates NRF2.
abstract: Apurinic/apyrimidinic endonuclease/redox factor-1 (APE1/Ref-1) (henceforth referred to as Ref-1) is a multifunctional protein that in addition to its base excision DNA repair activity exerts redox control of multiple transcription factors, including nuclear factor κ-light chain enhancer of activated B cells (NF-κB), STAT3, activator protein-1 (AP-1), hypoxia-inducible factor-1 (HIF-1), and tumor protein 53 (p53). In recent years, Ref-1 has emerged as a promising therapeutic target in cancer, particularly in pancreatic ductal carcinoma. Although a significant amount of research has centered on Ref-1, no wide-ranging approach had been performed on the effects of Ref-1 inhibition and transcription factor activity perturbation. Starting with a broader approach, we identified a previously unsuspected effect on the nuclear factor erythroid-related factor 2 (NRF2), a critical regulator of cellular defenses against oxidative stress. Based on genetic and small molecule inhibitor-based methodologies, we demonstrated that repression of Ref-1 potently activates NRF2 and its downstream targets in a dose-dependent fashion, and that the redox, rather than the DNA repair function of Ref-1 is critical for this effect. Intriguingly, our results also indicate that this pathway does not involve reactive oxygen species. The link between Ref-1 and NRF2 appears to be present in all cells tested in vitro, noncancerous and cancerous, including patient-derived tumor samples. In particular, we focused on understanding the implications of the novel interaction between these two pathways in primary pancreatic ductal adenocarcinoma tumor cells and provide the first evidence that this mechanism has implications for overcoming the resistance against experimental drugs targeting Ref-1 activity, with clear translational implications.
authors.affiliation: From the Departments of Pediatrics, Wells Center for Pediatric Research, Pharmacology and Toxicology, mfishel@iu.edu.
authors.forename: Melissa L
authors.initials: ML
authors.lastname: Fishel
authors.affiliation: Microbiology and Immunology.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
authors.affiliation: Medicine.
authors.forename: Cecilia M
authors.initials: CM
authors.lastname: Devlin
authors.affiliation: Pharmacology and Toxicology.
authors.forename: Derek P
authors.initials: DP
authors.lastname: Logsdon
authors.affiliation: From the Departments of Pediatrics, Wells Center for Pediatric Research.
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: From the Departments of Pediatrics, Wells Center for Pediatric Research, Pharmacology and Toxicology.
authors.forename: Meihua
authors.initials: M
authors.lastname: Luo
authors.affiliation: From the Departments of Pediatrics, Wells Center for Pediatric Research.
authors.forename: Ying
authors.initials: Y
authors.lastname: He
authors.affiliation: Biostatistics, and.
authors.forename: Zhangsheng
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Biostatistics, and.
authors.forename: Yan
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Pathology, Indiana University School of Medicine, Indianapolis, Indiana 46202 and.
authors.forename: Kelsey P
authors.initials: KP
authors.lastname: Lipking
authors.affiliation: the Departments of Oncology and Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: Anirban
authors.initials: A
authors.lastname: Maitra
authors.affiliation: the Departments of Oncology and Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
authors.forename: N V
authors.initials: NV
authors.lastname: Rajeshkumar
authors.affiliation: Medicine.
authors.forename: Glenda
authors.initials: G
authors.lastname: Scandura
authors.affiliation: From the Departments of Pediatrics, Wells Center for Pediatric Research, Pharmacology and Toxicology.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Kelley
authors.affiliation: Microbiology and Immunology, Medicine, mivan@iu.edu.
authors.forename: Mircea
authors.initials: M
authors.lastname: Ivan
mesh_terms: D045744:Cell Line, Tumor; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D017382:Reactive Oxygen Species; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
keywords: APE1/Ref-1; HMOX1; Hypoxia; Nuclear Factor 2 (Erythroid-derived 2-like Factor) (NFE2L2) (Nrf2); Pancreatic Adenocarcinoma; Reactive Oxygen Species (ROS); Redox Signaling; Transcription Factor