title: Autophagy in zebrafish.
abstract: From a hitherto underappreciated phenomenon, autophagy has become one of the most intensively studied cellular processes in recent years. Its role in cellular homeostasis, development and disease is supported by a fast growing body of evidence. Surprisingly, only a small fraction of new observations regarding the physiological functions of cellular "self-digestion" comes from zebrafish, one of the most popular vertebrate model organisms. Here we review the existing information about autophagy reporter lines, genetic knock-down assays and small molecular reagents that have been tested in this system. As we argue, some of these tools have to be used carefully due to possible pleiotropic effects. However, when applied rigorously, in combination with novel mutant strains and genome editing techniques, they could also transform zebrafish into an important animal model of autophagy research.
authors.affiliation: Department of Genetics, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Máté
authors.initials: M
authors.lastname: Varga
authors.affiliation: Department of Genetics, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Fodor
authors.affiliation: Department of Genetics, Eötvös Loránd University, Budapest, Hungary. Electronic address: vellai@falco.elte.hu.
authors.forename: Tibor
authors.initials: T
authors.lastname: Vellai
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D004195:Disease Models, Animal; D016678:Genome; D015027:Zebrafish
keywords: Autophagy; Morpholinos; Regeneration; Reporter lines; Small molecules; Zebrafish