title: Role of caveolin-3 in lymphocyte activation.
abstract: OBJECTIVE
  Caveolins are structural proteins clustered in lipid-rich regions of plasma membrane involved in coordinating signal transduction in various organ systems. While caveolin-1 (Cav-1) has been shown to regulate lymphocyte activation, the role of caveolin-3 (Cav-3) in immune system signaling has not been investigated. We tested the hypothesis that Cav-3 modulates lymphocyte activation.
  
  
  METHODS
  Lymphocyte/leukocyte subpopulations from WT and Cav-3 mice were profiled with flow cytometry. Cytokine production in quiescent and activated splenocytes from WT and Cav-3 mice was assessed with ELISA.
  
  
  RESULTS
  Levels of T-cells, monocytes, and natural killer cells were not different between WT and KO mice, however KO mice had lower B-cell population-percentage. Functionally, activated lymphocytes from Cav-3 KO mice demonstrated significantly reduced expression of IL-2 compared to WT, while expression of TNFα, IL-6, and IL-10 was not different. Finally, expression of IL-17 was significantly reduced in T-helper cells from KO mice, while IFNγ was not, suggesting that Cav-3 is a determinant in the development of the Th-17 subpopulation.
  
  
  CONCLUSIONS
  This study is the first to demonstrate that Cav-3 may be a novel participant in B-cell expression, T-cell cytokine production and activation of inflammation.
authors.affiliation: Department of Anesthesiology, Veterans Affairs San Diego Healthcare System, University of California, San Diego, La Jolla, CA, United States.
authors.forename: Chinh
authors.initials: C
authors.lastname: Tran
authors.affiliation: Department of Anesthesia, Stanford University, Stanford, CA, United States.
authors.forename: Creed M
authors.initials: CM
authors.lastname: Stary
authors.affiliation: Department of Anesthesiology, Veterans Affairs San Diego Healthcare System, University of California, San Diego, La Jolla, CA, United States.
authors.forename: Jan M
authors.initials: JM
authors.lastname: Schilling
authors.affiliation: Department of Anesthesiology, Veterans Affairs San Diego Healthcare System, University of California, San Diego, La Jolla, CA, United States.
authors.forename: Brandon
authors.initials: B
authors.lastname: Bentley
authors.affiliation: Department of Anesthesiology, Veterans Affairs San Diego Healthcare System, University of California, San Diego, La Jolla, CA, United States.
authors.forename: Hemal H
authors.initials: HH
authors.lastname: Patel
authors.affiliation: Department of Anesthesiology, Veterans Affairs San Diego Healthcare System, University of California, San Diego, La Jolla, CA, United States. Electronic address: droth@ucsd.edu.
authors.forename: David M
authors.initials: DM
authors.lastname: Roth
mesh_terms: D000818:Animals; D051244:Caveolin 3 / Q000235:genetics / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007107:Immune System / Q000502:physiology; D008213:Lymphocyte Activation / Q000502:physiology*; D018655:Lymphocyte Count; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction / Q000502:physiology
chemical_list: D051244:Caveolin 3; D016207:Cytokines
keywords: Cytokine; Immune system; Inflammation; Leukocyte; Signaling