title: Profiling of grape monoterpene glycosides (aroma precursors) by ultra-high performance-liquid chromatography-high resolution mass spectrometry (UHPLC/QTOF).
abstract: A 'suspect screening analysis' method for grape metabolomics by ultra-high performance-liquid chromatography (UHPLC) and high-resolution quadrupole-time of flight (QTOF) mass spectrometry was recently developed. This method was applied to study grape monoterpene glycosides, the main grape aroma precursors. Since standard compounds were not available, they were tentatively identified by overlapping various analytical approaches, in agreement with the indications recommended in mass spectrometry (MS)-based metabolomics. Accurate mass and isotopic pattern, MS/MS fragmentation, correlation between fragments observed and putative structures and between liquid chromatography coupled with mass spectrometry (LC/MS) and gas chromatography/mass spectrometry signals were studied. Seventeen monoterpene glycosides were identified without performing the hydrolytic artifacts commonly used to study these compounds which may affect sample profile. This is the first time that a detailed study of these aroma precursors has been carried out by direct LC/MS analysis.
authors.affiliation: Consiglio per la Ricerca e la Sperimentazione in Agricoltura, Centro di Ricerca per la Viticoltura (CRA-VIT), Viale XXVIII aprile 26, 31015, Conegliano, TV, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Flamini
authors.forename: Mirko
authors.initials: M
authors.lastname: De Rosso
authors.forename: Annarita
authors.initials: A
authors.lastname: Panighel
authors.forename: Antonio
authors.initials: A
authors.lastname: Dalla Vedova
authors.forename: Fabiola
authors.initials: F
authors.lastname: De Marchi
authors.forename: Luigi
authors.initials: L
authors.lastname: Bavaresco
keywords: aroma precursors; grape; metabolomics; monoterpene glycosides; time-of-flight mass spectrometry