title: Regulated Expression Systems for Mycobacteria and Their Applications.
abstract: For bacterial model organisms likeEscherichia coliandBacillus subtilisgenetic tools to experimentally manipulate the activity of individual genes existed for decades. But for genetically less tractable yet medically important bacteria such asM. tuberculosissuch tools have rarely been available. More recently several groups developed genetic switches that function efficiently inM. tuberculosisand other mycobacteria. Together these systems utilize six different transcription factors, eight different regulated promoters, and three different regulatory principles. Here we describe their design features, review their main applications, and discuss advantages and disadvantages of regulating transcription, translation, or protein stability for controlling gene activities in bacteria.
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College, and Program in Molecular Biology, Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10065.
authors.forename: Dirk
authors.initials: D
authors.lastname: Schnappinger
authors.affiliation: Department of Microbiology and Immunology, Weill Medical College, and Program in Immunology and Microbial Pathogenesis, Weill Graduate School of Medical Sciences of Cornell University, New York, NY 10065.
authors.forename: Sabine
authors.initials: S
authors.lastname: Ehrt