title: Promotion of cell adhesion by low-molecular-weight hydrogel by Lys based amphiphile.
abstract: Hydrogels formed by low-molecular hydrogelators have been used as anti-microbial agents and cell-attachment materials. However the biomedical application of low-molecular gelators is slowly progressing compared to the hydrogels formed by polymer hydrogelator that is applied to biomedical application such as tissue engineering and biomedical regions. To obtain a simple molecular model for potent and prospective usage of low-molecular hydrogelators, we designed a Lys-based hydrogelator which was mimic to the poly cationic poly-l-lysine that promotes cells to attach to a plastic plate nonspecifically. The gel-coating led to cause 10-fold cell attachment compared to no-coating well. Also five-time cells were attached to the well compared to the poly-l-lysine coating. From the competitive assay, these hydrogels could interact with cells through electrostatic interaction between positive charge from -NH3(+) in the hydrogelator and negative charge from substances on the cell surface such as glycosaminoglycans. This strong adhesive ability can be useful for the tissue engineering and molecular glue regions using low-molecular hydrogels in the future.
authors.affiliation: Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, 1 Honjo, Saga 840-8502, Japan.
authors.forename: Torao
authors.initials: T
authors.lastname: Suga
authors.affiliation: Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, 1 Honjo, Saga 840-8502, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Osada
authors.affiliation: Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, 1 Honjo, Saga 840-8502, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Narita
authors.affiliation: Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, 1 Honjo, Saga 840-8502, Japan.
authors.forename: Yushi
authors.initials: Y
authors.lastname: Oishi
authors.affiliation: Department of Chemistry and Applied Chemistry, Graduate School of Science and Engineering, Saga University, 1 Honjo, Saga 840-8502, Japan. Electronic address: hiroaki@cc.saga-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kodama
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry* / Q000494:pharmacology*; D019169:Jurkat Cells; D008239:Lysine / Q000737:chemistry* / Q000494:pharmacology*; D008970:Molecular Weight; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D055672:Static Electricity; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D011108:Polymers; D013501:Surface-Active Agents; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008239:Lysine
keywords: Cell attachment; Low-molecular hydrogel; Self-aggregation; Supramolecular hydrogelator