title: DNA nanoswitches: a quantitative platform for gel-based biomolecular interaction analysis.
abstract: We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions using a gel electrophoresis readout. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrated this low-cost, versatile, 'lab-on-a-molecule' system by characterizing ten different interactions, including a complex four-body interaction with five discernible states.
authors.affiliation: Program in Neuroscience, Department of Neurobiology, Harvard Medical School, Boston, MA, United States.
authors.forename: Mounir A
authors.initials: MA
authors.lastname: Koussa
authors.affiliation: The RNA Institute, University at Albany, Albany, NY, United States.|Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, United States.
authors.forename: Ken
authors.initials: K
authors.lastname: Halvorsen
authors.affiliation: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, United States.
authors.forename: Andrew
authors.initials: A
authors.lastname: Ward
authors.affiliation: Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, United States.|Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, United States.|Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, United States.
authors.forename: Wesley P
authors.initials: WP
authors.lastname: Wong
mesh_terms: D001710:Biotin / Q000737:chemistry; D004270:DNA, Circular / Q000737:chemistry* / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008024:Ligands; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D019809:Streptavidin / Q000737:chemistry
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D008024:Ligands; D011506:Proteins; D001710:Biotin; D019809:Streptavidin