title: Exercise Increases Markers of Spermatogenesis in Rats Selectively Bred for Low Running Capacity.
abstract: The oxidative stress effect of exercise training on testis function is under debate. In the present study we used a unique rat model system developed by artificial selection for low and high intrinsic running capacity (LCR and HCR, respectively) to evaluate the effects of exercise training on apoptosis and spermatogenesis in testis. Twenty-four 13-month-old male rats were assigned to four groups: control LCR (LCR-C), trained LCR (LCR-T), control HCR (HCR-C), and trained HCR (HCR-T). Ten key proteins connecting aerobic exercise capacity and general testes function were assessed, including those that are vital for mitochondrial biogenesis. The VO2 max of LCR-C group was about 30% lower than that of HCR-C rats, and the SIRT1 levels were also significantly lower than HCR-C. Twelve weeks of training significantly increased maximal oxygen consumption in LCR by nearly 40% whereas HCR remained unchanged. LCR-T had significantly higher levels of peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1α), decreased levels of reactive oxygen species and increased acetylated p53 compared to LCR-C, while training produced no significant changes for these measures in HCR rats. BAX and Blc-2 were not different among all four groups. The levels of outer dense fibers -1 (Odf-1), a marker of spermatogenesis, increased in LCR-T rats, but decreased in HCR-TR rats. Moreover, exercise training increased the levels of lactate dehydrogenase C (LDHC) only in LCR rats. These data suggest that rats with low inborn exercise capacity can increase whole body oxygen consumption and running exercise capacity with endurance training and, in turn, increase spermatogenesis function via reduction in ROS and heightened activity of p53 in testes.
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Torma
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Koltai
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Enikő
authors.initials: E
authors.lastname: Nagy
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Mohammad Mosaferi
authors.initials: MM
authors.lastname: Ziaaldini
authors.affiliation: Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary.
authors.forename: Aniko
authors.initials: A
authors.lastname: Posa
authors.affiliation: Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America.
authors.forename: Lauren G
authors.initials: LG
authors.lastname: Koch
authors.affiliation: Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America.
authors.forename: Steven L
authors.initials: SL
authors.lastname: Britton
authors.affiliation: Department of Microbiology and Immunology, Sealy Center for Molecular Medicine, University of Texas Medical Branch at Galveston, Galveston, Texas, United States of America.
authors.forename: Istvan
authors.initials: I
authors.lastname: Boldogh
authors.affiliation: Research Institute of Sport Science, Semmelweis University, Budapest, Hungary.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Radak
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism*; D008297:Male; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012420:Running; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D017382:Reactive Oxygen Species