title: Enantioseparation of Three Important Intermediates of Tanikolide with Immobilized Cellulose Chiral Stationary Phase.
abstract: Three stereoselective HPLC methods have been developed for the chiral separation of the enantiomers of three intermediates in the preparation of (+)-tanikolide. The enantiomers were separated on Chiralpak IC (250 × 4.6 mm, 5 μm) in normal phase HPLC. Three intermediates were all baseline separated (RS = 2.84, 2.58 and 3.58, respectively). By comparing the chromatograms of racemates and single enantiomers of the three intermediates, the e.e. values of the single enantiomers were determined and calculated.
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China Key Laboratory of State Ministry of Education for Pharmaceutical Technology, Zhengzhou, Henan 450001, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Jie
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Du
authors.initials: D
authors.lastname: Qiuzheng
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Suzhen
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Sun
authors.initials: S
authors.lastname: Fang
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xinyu
authors.affiliation: School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, PR China Key Laboratory of State Ministry of Education for Pharmaceutical Technology, Zhengzhou, Henan 450001, PR China zhangzz08@126.com.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zhenzhong
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol; D007783:Lactones / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D013237:Stereoisomerism; D013696:Temperature
chemical_list: D007783:Lactones; C400156:tanikolide; D000431:Ethanol; D002482:Cellulose