title: Isolation and identification of a 10-deacetyl baccatin-III-producing endophyte from Taxus wallichiana.
abstract: Endophytic fungi of inner root bark of Taxus wallichiana var. mairei were investigated in order to find endophytes producing 10-DABIII (10-deacetyl baccatin III). Purified colonies were cultured in potato dextrose broth (PDB), and then the organic extracts from fungi were analyzed with HPLC, LC-MS, and (1)H NMR. Of 102 fungal endophytes isolated from the inner root bark, only one strain named IRB54 can yield 10-DABIII but no taxol and baccatin III. In PDB culture medium, its productivity was 187.564 ug/l. Based on its morphological characteristics and molecular data, the IRB54 strain was identified as Trichoderma sp. The isolation of the fungus IRB54 yielding 10-DABIII will provide an alternative resource to manufacture taxol/taxotere via semi-synthesis and some useful clues for improving the understanding of taxane synthesis evolution.
authors.affiliation: School of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xiuren
authors.initials: X
authors.lastname: Zhou
authors.forename: Wenen
authors.initials: W
authors.lastname: Zhao
authors.forename: Zaiyou
authors.initials: Z
authors.lastname: Jian
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics*; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D043823:Taxoids / Q000302:isolation & purification* / Q000378:metabolism; D020946:Taxus / Q000382:microbiology*; D014242:Trichoderma / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004271:DNA, Fungal; D043823:Taxoids; C042213:10-deacetylbaccatine III