title: TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation.
abstract: The cellular functions of the trans-Golgi network protein TGN38 remain unknown. In this research, we studied the expression, localization and functions of TGN38 in the meiotic maturation of mouse oocytes. TGN38 was expressed at every stage of oocyte meiotic maturation and colocalized with γ-tubulin at metaphase I and metaphase II. The spindle microtubule disturbing agents nocodazole and taxol did not affect the colocalization of TGN38 and γ-tubulin. Depletion of TGN38 with specific siRNAs resulted in increased metaphase I arrest, accompanied with spindle assembly checkpoint activation and decreased first polar extrusion (PB1). In the oocytes that had extruded the PB1 after the depletion of TGN38, symmetric division occurred, leading to the production of 2 similarly sized cells. Moreover, the peripheral migration of metaphase I spindle and actin cap formation were impaired in TGN38-depleted oocytes. Our data suggest that TGN38 may regulate the metaphase I/anaphase I transition and asymmetric cell division in mouse oocytes.
authors.affiliation: a Reproductive Medicine Center ; First Affiliated Hospital of Zhengzhou University ; Zhengzhou , Henan Province , China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Zhao-Jia
authors.initials: ZJ
authors.lastname: Ge
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
authors.forename: Tianyi
authors.initials: T
authors.lastname: Sun
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.forename: Ying-Pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D000199:Actins / Q000378:metabolism; D000705:Anaphase / Q000187:drug effects; D000818:Animals; D060049:Asymmetric Cell Division / Q000187:drug effects; D005260:Female; D055785:Gene Knockdown Techniques; D008540:Meiosis / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008677:Metaphase / Q000187:drug effects; D008813:Mice, Inbred ICR; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D059705:Polar Bodies / Q000166:cytology / Q000187:drug effects; D021381:Protein Transport; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D000199:Actins; D008562:Membrane Glycoproteins; D034741:RNA, Small Interfering; C513047:Tgoln1 protein, mouse; D017239:Paclitaxel; D015739:Nocodazole
keywords: GV, germinal vesicle; GVBD, germinal vesicle breakdown; Golgi apparatus; MI, metaphase of the first meiosis; MII, metaphase of the second meiosis; MTOCs, microtubule organizing centers; PB1, first polar body extrusion; TGN, trans-Golgi network; TGN38; asymmetric cell division; metaphase I/anaphase I transition