title: Fumigant activity of the Psidium guajava var. pomifera (Myrtaceae) essential oil in Drosophila melanogaster by means of oxidative stress.
abstract: The guava fruit, Psidium guajava var. pomifera (Myrtaceae family), is a native plant from South America. Its leaves and fruits are widely used in popular medicine in tropical and subtropical countries. Drosophila melanogaster has been used as one of the main model organisms in genetic studies since the 1900s. The extensive knowledge about this species makes it one of the most suitable organisms to study many aspects of toxic compound effects. Due to the lack of studies on the effects of the bioactive compounds present in the P. guajava var. pomifera essential oil, we performed a phytochemical characterization by CG-MS and evaluated the toxicity induced by the essential oil in the D. melanogaster insect model. In order to understand the biochemical mechanisms of toxicity, changes on the Nrf2 signaling as well as hallmarks of oxidative stress response were followed in the exposed flies. Our results showed that exposure of insects to the P. guajava oil increased mortality and locomotor deficits in parallel with an oxidative stress response signaling. Therefore, it suggested a bioinsecticidal activity for P. guajava volatile compounds by means of oxidative stress. Further studies are ongoing to identify which oil compounds are responsible for such effect.
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Antonio Ivanildo
authors.initials: AI
authors.lastname: Pinho
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIPBIOTEC), Universidade Federal do Pampa, Campus São Gabriel, Avenida Av Antonio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Gabriel Luz
authors.initials: GL
authors.lastname: Wallau
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIPBIOTEC), Universidade Federal do Pampa, Campus São Gabriel, Avenida Av Antonio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Mauro Eugenio Medina
authors.initials: ME
authors.lastname: Nunes
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Nadghia Figueiredo
authors.initials: NF
authors.lastname: Leite
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Saulo Relison
authors.initials: SR
authors.lastname: Tintino
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIPBIOTEC), Universidade Federal do Pampa, Campus São Gabriel, Avenida Av Antonio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Litiele Cezar
authors.initials: LC
authors.lastname: da Cruz
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Francisco Assis Bezerra
authors.initials: FA
authors.lastname: da Cunha
authors.affiliation: Laboratório de Pesquisa em Produtos Naturais, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: José Galberto Martins
authors.initials: JG
authors.lastname: da Costa
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, 63105-000 Crato, CE, Brazil.
authors.forename: Henrique
authors.initials: H
authors.lastname: Douglas Melo Coutinho
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIPBIOTEC), Universidade Federal do Pampa, Campus São Gabriel, Avenida Av Antonio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Thais
authors.initials: T
authors.lastname: Posser
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia (CIPBIOTEC), Universidade Federal do Pampa, Campus São Gabriel, Avenida Av Antonio Trilha 1847, Centro, 97300-000 São Gabriel, RS, Brazil.
authors.forename: Jeferson Luis
authors.identifier: 0000-0002-3874-8618
authors.initials: JL
authors.lastname: Franco
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000378:metabolism*; D005260:Female; D005651:Fumigation / Q000379:methods; D008297:Male; D027822:Myrtaceae / Q000737:chemistry*; D009822:Oils, Volatile / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D031647:Psidium / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts