title: Complement activation in Glioblastoma multiforme pathophysiology: evidence from serum levels and presence of complement activation products in tumor tissue.
abstract: Inflammation plays a key role in the pathophysiology of Glioblastoma Multiforme (GBM). Here we focus on the contribution of the so far largely ignored complement system. ELISA and immunohistochemistry were combined to assess levels and localization of critical components of the initiation- and effector pathways of the complement cascade in sera and tumor tissue from GBM patients and matched controls. Serum levels of factor-B were decreased in GBM patients whereas C1q levels were increased. C1q and factor-B deposited in the tumor tissue. Deposition of C3 and C5b-9 suggests local complement activation.MBL deficiency, based on serum levels, was significantly less frequent among GBM patients compared to controls (14% vs. 33%). Therefore low levels of MBL may protect against the initiation/progression of GBM.
authors.affiliation: Erasmus University Medical Center, Brain Tumor Center, Department of Neurosurgery, Dr. Molewaterplein 50, 3015 GE Rotterdam, Netherlands.
authors.forename: T A M
authors.initials: TA
authors.lastname: Bouwens
authors.affiliation: Leiden University Medical Center, Department of Rheumatology, PO Box 9600, 2300 RC Leiden, Netherlands.
authors.forename: L A
authors.initials: LA
authors.lastname: Trouw
authors.affiliation: Vrije University Medical Center, Departments of Clinical Chemistry and Psychiatry, PO Box 7057, 1007 MB Amsterdam, Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Veerhuis
authors.affiliation: Erasmus University Medical Center, Brain Tumor Center, Department of Neurosurgery, Dr. Molewaterplein 50, 3015 GE Rotterdam, Netherlands.
authors.forename: C M F
authors.initials: CM
authors.lastname: Dirven
authors.affiliation: Erasmus University Medical Center, Brain Tumor Center, Department of Neurosurgery, Dr. Molewaterplein 50, 3015 GE Rotterdam, Netherlands.
authors.forename: M L M
authors.initials: ML
authors.lastname: Lamfers
authors.affiliation: Erasmus University Medical Center, Brain Tumor Center, Department of Neurosurgery, Dr. Molewaterplein 50, 3015 GE Rotterdam, Netherlands. Electronic address: h.alkhawaja@erasmusmc.nl.
authors.forename: H
authors.initials: H
authors.lastname: Al-Khawaja
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D003167:Complement Activation; D015922:Complement C1q / Q000378:metabolism; D011415:Complement Factor B / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005909:Glioblastoma / Q000097:blood / Q000276:immunology / Q000503:physiopathology; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000378:metabolism; D008875:Middle Aged; D016019:Survival Analysis
chemical_list: D037601:Mannose-Binding Lectin; D015922:Complement C1q; D011415:Complement Factor B
keywords: C1q; Complement system; Factor B; Glial tumor; Glioblastoma Multiforme; Mannose Binding Lectin (MBL)