title: Germacrane sesquiterpenes isolated from the rhizome of Curcuma xanthorrhiza Roxb. inhibit UVB-induced upregulation of MMP-1, -2, and -3 expression in human keratinocytes.
abstract: Four sesquiterpenes were isolated from the rhizome of Curcuma xanthorrhiza Roxb.: furanodiene (1), germacrone (2), furanodienone (3), and 13-hydroxygermacrone (4). Importantly, this was the first time compounds 1 and 4 were isolated from this plant. The chemical structures of these compounds were determined using 1D- and 2D-nuclear magnetic resonance, infrared spectroscopy, and electron ionization mass spectrometry analyses. Among the isolated compounds, compounds 2 and 4 inhibited UVB-induced upregulation of the mRNA and protein expression levels of MMP-1, MMP-2, and MMP-3 in human keratinocytes (HaCaT). Moreover, this upregulation occurred in a dose-dependent manner over the range of 1-10 μM for each compound.
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Ji-Hae
authors.initials: JH
authors.lastname: Park
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Mohamed Antar Aziz
authors.initials: MA
authors.lastname: Mohamed
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Ye-Jin
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Sabina
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Division of Metabolism and Functionality Research, Korea Food Research Institute, Songnam, 463-746, Republic of Korea.
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Division of Metabolism and Functionality Research, Korea Food Research Institute, Songnam, 463-746, Republic of Korea.
authors.forename: Daeseok
authors.initials: D
authors.lastname: Han
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, Institute of Life Sciences & Resources, Kyung Hee University, Yongin, 446-701, Republic of Korea. nibaek@khu.ac.kr.
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D002478:Cells, Cultured; D030024:Curcuma / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D009682:Magnetic Resonance Spectroscopy; D020781:Matrix Metalloproteinase 1 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D027343:Rhizome; D045788:Sesquiterpenes, Germacrane / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects* / Q000528:radiation effects
chemical_list: D012333:RNA, Messenger; D045788:Sesquiterpenes, Germacrane; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1
keywords: Curcuma xanthorrhiza; Germacrane; HaCaT; MMPs; NMR; UVB