title: ADAMTS-4 and ADAMTS-5 expression in human temporomandibular joint discs with internal derangement, correlates with degeneration.
abstract: Temporomandibular joint (TMJ) internal derangement (ID) is one of the most common form of temporomandibular disorders. There is evidence showing the increased expression of matrix metalloproteinases (MMPs) in the cells from degenerated TMJ disc. ADAMTS are a large family of metalloproteases which are responsible for proteoglycans degradation. The present study aimed to evaluate ADAMTS-4 and ADAMTS-5 immunohistochemical expression in human TMJ discs from patients affected by ID, and to find out if there is any correlation with the degree of histopathological changes. Eighteen temporomandibular displaced disc specimens and sixteen TMJ disc control were used for the present study. Specimens were immunohistochemically processed and ADAMTS-4 and ADAMTS-5 expression were obtained respectively for the anterior (AB), intermediate (IB) and posterior (PB) bands and compared to the histopathological degeneration score (HDS). Immunoreactivity for ADAMTS-4 and -5, was observed in both not degenerated and degenerated human TMJ discs. Both the percentage of ADAMTS-4 and -5 immunostained cells (ES) and the intensity of staining (IS) were significantly greater in affected specimens compared with those in control discs. The ADAMTS-5 ES and IS of the 3 bands of the disc correlated to the TMJ disc HDS (0.001 < P < 0.05), on the other hand only AB and IB, ADAMTS-4 immunostaining scores correlated to HDS. According to these findings it can be assumed in that the more histopathological changes in the disc are detected, the higher levels of ADAMTS are produced. This in turn can lead to ECM breakdown and in turn to a more advanced disc displacement.
authors.affiliation: Department of Orthodontics and Orofacial Pain, University of Catania, Catania, Italy.
authors.forename: Rosalia
authors.initials: R
authors.lastname: Leonardi
authors.affiliation: Department of Maxillofacial Surgery, University of Messina, Messina, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Crimi
authors.affiliation: Department of Oral Surgery, Marquette University, Milwaukee, WI, USA.
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Almeida
authors.affiliation: Department of Surgical Sciences, Institute of Pathology and Cytopathology, University of Foggia, Foggia, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Pannone
authors.affiliation: Department of Bio-Medical Sciences, Anatomy Section, University of Catania, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Musumeci
authors.affiliation: Department of Bio-Medical Sciences, Anatomy Section, University of Catania, Catania, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Castorina
authors.affiliation: Division of Anatomy, Faculty of Dental Medicine, 'Carol Davila' University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: Mugurel Constantin
authors.initials: MC
authors.lastname: Rusu
authors.affiliation: Department of Bio-Medical Sciences, Anatomy Section, University of Catania, Catania, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
mesh_terms: D051722:ADAM Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D000071121:ADAMTS4 Protein; D000071116:ADAMTS5 Protein; D000293:Adolescent; D000328:Adult; D000818:Animals; D000906:Antibodies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D004204:Joint Dislocations / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011348:Procollagen N-Endopeptidase / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D019224:Temporomandibular Joint Disc / Q000201:enzymology* / Q000473:pathology* / Q000601:surgery; D013705:Temporomandibular Joint Disorders / Q000201:enzymology* / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D000906:Antibodies; D051722:ADAM Proteins; D000071116:ADAMTS5 Protein; C121532:ADAMTS5 protein, human; D011348:Procollagen N-Endopeptidase; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human
keywords: matrix metalloproteinases; temporomandibular joint disorders