title: The tomato ubiquitin-conjugating enzyme variant Suv, but not SlUev1C and SlUev1D regulates Fen-mediated programmed cell death in Nicotiana benthamiana.
abstract: The unconventional, lysine-63-linked ubiquitination has been shown to play a central role in regulating human and animal innate and adaptive immunity. By contrast, the role and mechanism of K63-linked ubiquitination in plant biology remain largely unexplored. The tomato (Solanum lycopersicum) Fni3 ubiquitin-conjugating enzyme and its co-factor, Suv ubiquitin E2 variant (Uev) were shown recently to catalyze K63-linked ubiquitination and are essential for protein Fen and other resistance protein-mediated plant immunity. In this study we detected the subcellular localization of Fen, Fni3 and Suv and confirmed the interaction of Fni3 with Suv in tomato protoplasts. Additionally, we identified 2 tomato Uev1 homologs, SlUev1C and SlUev1D, respectively and showed they are not required for Fen-mediated programmed cell death in Nicotiana benthamiana, suggesting Uev homologs play differential role in the cell.
authors.affiliation: a Biology Department ; University of Arkansas ; Little Rock , AR USA.
authors.forename: Sadia
authors.initials: S
authors.lastname: Hamera
authors.forename: Ravi M
authors.initials: RM
authors.lastname: Mural
authors.forename: Yao
authors.initials: Y
authors.lastname: Liu
authors.forename: Lirong
authors.initials: L
authors.lastname: Zeng
mesh_terms: D017209:Apoptosis; D001483:Base Sequence; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D018551:Solanum lycopersicum / Q000201:enzymology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics; D044763:Ubiquitin-Conjugating Enzymes / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D044763:Ubiquitin-Conjugating Enzymes
keywords: K63-linked; Uev; cell death; plant immunity; ubiquitination