title: Poly(ADP-ribosyl)ation is recognized by ECT2 during mitosis.
abstract: Poly(ADP-ribosyl)ation is an unique posttranslational modification and required for spindle assembly and function during mitosis. However, the molecular mechanism of poly(ADP-ribose) (PAR) in mitosis remains elusive. Here, we show the evidence that PAR is recognized by ECT2, a key guanine nucleotide exchange factor in mitosis. The BRCT domain of ECT2 directly binds to PAR both in vitro and in vivo. We further found that α-tubulin is PARylated during mitosis. PARylation of α-tubulin is recognized by ECT2 and recruits ECT2 to mitotic spindle for completing mitosis. Taken together, our study reveals a novel mechanism by which PAR regulates mitosis.
authors.affiliation: a Division of Molecular Medicine and Genetics ; Department of Internal Medicine ; University of Michigan Medical School ; Ann Arbor , MI USA.
authors.forename: Mo
authors.initials: M
authors.lastname: Li
authors.forename: Chunjing
authors.initials: C
authors.lastname: Bian
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Yu
mesh_terms: D001665:Binding Sites; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D008954:Models, Biological; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000378:metabolism; D038501:Tankyrases / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D011064:Poly Adenosine Diphosphate Ribose; D011518:Proto-Oncogene Proteins; D038501:Tankyrases; D014404:Tubulin; C489850:ECT2 protein, human; C418109:TNKS protein, human
keywords: Abbreviations: PAR; BRCA1 C Terminus; CHFR; BRCA1-associated RING domain protein 1; BRCT; BRCT domain; Breast Cancer Gene 1; BARD1; Dbl homology; PH; ECT2; Ethylenediaminetetraacetic acid; GST; PAR; PAR binding; Poly(ADP-ribose) polymerase 1; RhoGEF; Rho guanine nucleotide exchange factor; TBST; Tris-Buffered Saline and Tween 20.; checkpoint with forkhead-associated and ring finger; DH; epithelial cell transforming sequence 2 oncogene; BRCA1; glutatione S-transferase; PARG; mitosis; pleckstrin homology; PBC; poly(ADP-ribose) glycohydrolase gene; PARP-1; poly(ADP-ribose); PARylation; poly(ADP-ribosyl)ation; ECT2; polybasic cluster; EDTA