title: Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata Delile, a marine macro alga.
abstract: Bioprospecting of marine natural products has recently produced a substantial number of drug candidates. Ulva fasciata Delile, belonging to the family Ulvaceae, is a green marine macro alga that grows profusely on the coastal seashore of South India. In the present study, we investigated the in vitro cytotoxic potential of a methanolic extract of U . fasciata Delile (MEUF) using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against human colon carcinoma (HT-29), human hepatocyte carcinoma (Hep-G2), and human breast carcinoma (MCF-7) cell lines. Response surface methodology (RSM) was applied using central-composite experimental design (CCD) to obtain optimum combined effect of concentration and cancer cells with highest cytotoxicity. The effect of concentration, cancer cell lines as independent variables on absorbance (OD), percent cell survival and percent cell inhibition as dependent variables was investigated. Maximum cytotoxic activity of MEUF was established for Hep-G2 with lowest OD or percent cell survival; highest percent cell inhibition with significant difference (p > 0.05) was compared to HT-29 and MCF-7.
authors.affiliation: Raghu College of Pharmacy, Dakamarri, Bheemili (M), Visakhapatnam 531162, Andhra Pradesh, India.
authors.forename: Mukesh Kumar
authors.initials: MK
authors.lastname: Das
authors.affiliation: Raghu College of Pharmacy, Dakamarri, Bheemili (M), Visakhapatnam 531162, Andhra Pradesh, India.
authors.forename: Prafulla Kumar
authors.initials: PK
authors.lastname: Sahu
authors.affiliation: Saastra College of Pharmaceutical Education and Research, Varigonda, Nellore 524311, Andhra Pradesh, India.
authors.forename: G Srinivasa
authors.initials: GS
authors.lastname: Rao
authors.affiliation: Institute of Science and Technology, JNT University, Kukutpally, Hyderabad 500072, Andhra Pradesh, India.
authors.forename: K
authors.initials: K
authors.lastname: Mukkanti
authors.affiliation: Avanthi Institute of Pharmaceutical Sciences, Cherukupalli, Vizianagaram 261152, Andhra Pradesh, India.
authors.forename: L
authors.initials: L
authors.lastname: Silpavathi
keywords: Cytotoxicity; Green marine macro alga; MTT assay; Response surface methodology; Ulva fasciata Delile