title: Application of confocal Raman microscopy to investigate casein micro-particles in blend casein/pectin films.
abstract: Pectin triggers formation of casein micro-particles during solution casting. Confocal Raman microscopy revealed their composition and spatial dimension in resulting films. Peaks in the Raman spectra corresponded to those found in films prepared by either casein or pectin. This suggested that no conformational changes occurred after mixing. Raman images revealed incompatibility of both polymers because particles consisted of casein only and the surrounding matrix of pectin. Deformation of micro-particles into an oblate shape took place during film formation. In dried films, an empty space between casein and pectin was found in lateral dimension. In contrast, casein micro-particles overlapped with the pectin matrix in the vertical dimension.
authors.affiliation: Chair of Food Process Engineering and Dairy Technology, Technische Universität München, 85354 Freising-Weihenstephan, Germany.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Chair of Food Packaging Technology, Technische Universität München, 85354 Freising, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Sterr
authors.affiliation: Research Centre for Nutrition and Food Science (ZIEL) - Section Technology, Technische Universität München, 85354 Freising-Weihenstephan, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kulozik
authors.affiliation: Chair of Food Process Engineering and Dairy Technology, Technische Universität München, 85354 Freising-Weihenstephan, Germany. Electronic address: ronald.gebhardt@tum.de.
authors.forename: Ronald
authors.initials: R
authors.lastname: Gebhardt
mesh_terms: D002364:Caseins / Q000737:chemistry*; D008823:Micelles; D018613:Microscopy, Confocal; D010368:Pectins / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D002364:Caseins; D008823:Micelles; D010368:Pectins
keywords: Blend films; Casein; Micro-particle; Particle deformation; Raman microscopy