title: Association of genetic polymorphisms for glutathione peroxidase genes with obesity in Spanish children.
abstract: OBJECTIVE
  Altered expression and activity of the antioxidant enzymes glutathione peroxidases (GPXs) have been observed in obesity in human and animal studies. We aimed to study 59 single nucleotide polymorphisms (SNPs) for GPX1-7 genes and to characterize their association with prepubertal childhood obesity and its associated biomarkers.
  
  
  METHODS
  This case-control study included 193 obese and 191 normal-weight prepubertal Spanish children, in whom anthropometry, biochemical parameters, adipokines, antioxidant enzyme erythrocyte activities and biomarkers of oxidative stress, inflammation and cardiovascular risk were measured. The genotype analysis was performed in the Illumina platform. PLINK and SPSS were used for statistical analyses.
  
  
  RESULTS
  We found SNPs rs757228 and rs8103188 (GPX4) to be negatively associated and rs445870 (GPX5) and rs406113 (GPX6) to be positively associated with obesity in children. The variant rs2074451 (GPX4) increased GPX activity in erythrocytes. Although we did not find significant differences in erythrocyte GPX activity between obese and normal-weight children, GPX activity was found to be positively and significantly correlated with blood pressure, adipocyte fatty acid-binding protein and high-sensitivity C-reactive protein.
  
  
  CONCLUSIONS
  The GPX variants rs757228, rs8103188, rs445870 and rs406113 were associated with prepubertal childhood obesity. However, erythrocyte GPX activity was not altered in obese prepubertal Spanish children.
authors.affiliation: Department of Biochemistry and Molecular Biology II, Institute of Nutrition and Food Technology, Centre of Biomedical Research, University of Granada, Armilla, Spain.
authors.forename: Azahara I
authors.initials: AI
authors.lastname: Rupérez
authors.forename: Josune
authors.initials: J
authors.lastname: Olza
authors.forename: Mercedes
authors.initials: M
authors.lastname: Gil-Campos
authors.forename: Rosaura
authors.initials: R
authors.lastname: Leis
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Mesa
authors.forename: Rafael
authors.initials: R
authors.lastname: Tojo
authors.forename: Ramón
authors.initials: R
authors.lastname: Cañete
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gil
authors.forename: Concepción M
authors.initials: CM
authors.lastname: Aguilera
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D008297:Male; D063766:Pediatric Obesity / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D013030:Spain / Q000453:epidemiology
chemical_list: D005979:Glutathione Peroxidase