title: CYP2B6 gene single-nucleotide polymorphisms in an Italian population sample and relationship with nicotine dependence.
abstract: The extensively polymorphic CYP2B6 gene metabolizes endogenous and exogenous compounds, among which are nicotine and bupropion, although its contribution to the systemic metabolism of nicotine still remains controversial. In the present study, the distribution of the CYP2B6 variant and genotype frequencies were analyzed in a sample of 202 Italian individuals who were also invited to answer the Fagerström test for nicotine dependence (FTND), in an effort to assess the involvement of CYP2B6 polymorphisms in nicotine dependence. Eight single-nucleotide polymorphisms of CYP2B6 were tested and seven different variants were identified showing frequencies similar to the European population. The reduced activity of the CYP2B6*6 variant was significantly (p=0.025) distributed among the nicotine-dependent individuals compared to non-nicotine dependents. Also, the CYP2B6*1/*6 genotype achieved statistical significance (p=0.016) within the nicotine-dependent individuals. The high occurrence of CYP2B6*6 carriers among nicotine-dependent individuals may suggest a possible involvement in nicotine dependence, with a potential impact on smoking cessation treatments tailored to the individual smoker's genotype.
authors.affiliation: 1 Department of Medical and Surgical Sciences (DIMEC), Institute of Legal Medicine, University of Bologna , Bologna, Italy .
authors.forename: Laura Natalia
authors.initials: LN
authors.lastname: Riccardi
authors.forename: Francesco
authors.initials: F
authors.lastname: Carano
authors.forename: Carla
authors.initials: C
authors.lastname: Bini
authors.forename: Stefania
authors.initials: S
authors.lastname: Ceccardi
authors.forename: Gianmarco
authors.initials: G
authors.lastname: Ferri
authors.forename: Susi
authors.initials: S
authors.lastname: Pelotti
mesh_terms: D000483:Alleles; D001711:Biotransformation / Q000235:genetics; D065702:Cytochrome P-450 CYP2B6 / Q000235:genetics* / Q000502:physiology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007558:Italy / Q000453:epidemiology; D020641:Polymorphism, Single Nucleotide; D012494:Sampling Studies; D011795:Surveys and Questionnaires; D014029:Tobacco Use Disorder / Q000453:epidemiology / Q000235:genetics*
chemical_list: C585599:CYP2B6 protein, human; D065702:Cytochrome P-450 CYP2B6