title: The neutralizing antibody GNbAC1 abrogates HERV-W envelope protein-mediated oligodendroglial maturation blockade.
abstract: BACKGROUND
  The envelope protein (ENV) of the human endogenous retrovirus type W is implicated in inflammatory reactions in multiple sclerosis (MS) but also interferes with oligodendroglial maturation. A neutralizing antibody GNbAC1 has been developed and successfully been tested in clinical trials.
  
  
  OBJECTIVE
  We stimulated primary oligodendroglial cells with ENV upon preincubation with GNbAC1 and assessed for nitrosative stress and myelin expression.
  
  
  RESULTS
  Neutralization of ENV by GNbAC1 reduces its ability to induce stress reactions resulting in a rescue of myelin expression.
  
  
  CONCLUSIONS
  Beyond immune cell modulation, this monoclonal antibody may therefore help to overcome the oligodendroglial differentiation blockade in MS.
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany/These authors contributed equally to this study.
authors.forename: David
authors.initials: D
authors.lastname: Kremer
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany/These authors contributed equally to this study.
authors.forename: Moritz
authors.initials: M
authors.lastname: Förster
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Schichel
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Göttle
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Hartung
authors.affiliation: GeNeuro, Geneva, Switzerland.
authors.forename: Hervé
authors.initials: H
authors.lastname: Perron
authors.affiliation: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany kuery@uni-duesseldorf.de.
authors.forename: Patrick
authors.initials: P
authors.lastname: Küry
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D020077:Endogenous Retroviruses; D006801:Humans; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects* / Q000821:virology; D014759:Viral Envelope Proteins
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D057134:Antibodies, Neutralizing; D014759:Viral Envelope Proteins; C581064:temelimab
keywords: Myelin repair; antibody; human endogenous retrovirus; multiple sclerosis-associated retrovirus; nitrosative stress; oligodendrocytes; regeneration; therapy