title: The alteration of SHARPIN expression in the mouse brainstem during herpes simplex virus 1-induced facial palsy.
abstract: Bell's palsy presents a unilateral weakness or paralysis of the face due to acute dysfunction of the peripheral facial nerve with no readily identifiable cause. Although data show that herpes simplex virus type 1 (HSV-1) may be the possible causative agent of Bell's palsy, the precise mechanism of the paralysis is still unknown. SHANK-associated RH domain-interacting protein (SHARPIN) is thought to play a role in the control of inflammatory responses. In order to clarify the molecular pathway of SHARPIN involved in the facial palsy caused by HSV-1 in mice and the inhibitory effect of corticosteroids, we used 4-week-old Balb/c mice inoculated with HSV-1 for experiments. The expression and location of SHARPIN in the facial nucleus of brainstem were detected respectively by quantitative real-time polymerase chain reaction, western blot and immunofluorescence. Expression level of SHARPIN increased and peaked at 2 days and then decreased in the facial nucleus of brainstem after the manifestation of the facial paralysis. After the administration of MPSS, the protein expression of SHARPIN at the peak point was down-regulated. Our results suggest that SHRPIN were activated during the inflammatory reaction in the HSV-1-induced facial paralysis. MPSS can effectively inhibit the expression of SHARPIN that may contribute to attenuate HSV-1-mediated nervous system damage.
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Otology, Jinan, China; Institute of Eye and ENT, Provincial Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Li
authors.affiliation: Shandong Provincial Key Laboratory of Otology, Jinan, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Liu
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Yuechen
authors.initials: Y
authors.lastname: Han
authors.affiliation: Otolaryngology-Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, China; Shandong Provincial Key Laboratory of Otology, Jinan, China; Institute of Eye and ENT, Provincial Hospital Affiliated to Shandong University, Jinan, China. Electronic address: whbotologic797@163.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005158:Facial Paralysis / Q000378:metabolism* / Q000821:virology; D006561:Herpes Simplex / Q000378:metabolism* / Q000821:virology; D018259:Herpesvirus 1, Human; D047908:Intracellular Signaling Peptides and Proteins; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C554944:Sipl1 protein, mouse
keywords: Facial paralysis; Herpes simplex virus type 1 (HSV-1); MPSS; RU486; SHARPIN