title: A competitive strategy based on cucurbit[7]uril supramolecular interaction for simple and sensitive detection of dibucaine.
abstract: In this work, the competitive interaction between dibucaine and three fluorescent probes (i.e., berberine, palmatine, and coptisine) for occupancy of the cucurbit[7]uril (CB[7]) cavity was studied by fluorescence spectra, UV-visible absorption spectra, (1)H NMR spectra, and theoretical calculations in acidic aqueous solution. Based on the fluorescence enhancement of berberine, palmatine, and coptisine upon binding with CB[7], respectively, a series of fluorescence detection methods for dibucaine were proposed. At the optimized conditions, the fluorescence intensity of berberine-CB[7], palmatine-CB[7], and coptisine-CB[7] complexes showed negative correlation to the concentration of dibucaine, which led to a series of simple and sensitive fluorescence methods for the determination of dibucaine for the first time. Linear ranges obtained in the detection of the dibucaine were 0.018-3.34 μmol L(-1), 0.032-4.47 μmol L(-1), and 0.079-4.42 μmol L(-1) with detection limits of 6.0 nmol L(-1), 12.0 nmol L(-1), and 25.0 nmol L(-1), respectively. Moreover, the proposed method was successfully applied for the determination of the drug in biological fluids. The competitive mode based on CB[7] superstructure provided a promising assay strategy for fluorescence detection in various potential applications.
authors.affiliation: Analytical and Testing Center, Shanxi Normal University, Shanxi, Linfen 041004, PR China; School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, PR China. Electronic address: lichangfeng2@aliyun.com.
authors.forename: Chang-Feng
authors.initials: CF
authors.lastname: Li
authors.affiliation: Analytical and Testing Center, Shanxi Normal University, Shanxi, Linfen 041004, PR China. Electronic address: lmd@dns.sxnu.edu.cn.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Du
authors.affiliation: Analytical and Testing Center, Shanxi Normal University, Shanxi, Linfen 041004, PR China.
authors.forename: Jian-Xia
authors.initials: JX
authors.lastname: Feng
authors.affiliation: Analytical and Testing Center, Shanxi Normal University, Shanxi, Linfen 041004, PR China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Liu
authors.affiliation: School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, PR China.
authors.forename: Yun-Long
authors.initials: YL
authors.lastname: Fu
mesh_terms: D000779:Anesthetics, Local / Q000652:urine*; D001599:Berberine / Q000031:analogs & derivatives / Q000737:chemistry; D001600:Berberine Alkaloids / Q000737:chemistry; D001667:Binding, Competitive; D001952:Bridged-Ring Compounds / Q000737:chemistry*; D003992:Dibucaine / Q000652:urine*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D007700:Kinetics; D057230:Limit of Detection; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
chemical_list: D000779:Anesthetics, Local; D001599:Berberine; D001600:Berberine Alkaloids; D001952:Bridged-Ring Compounds; D003992:Dibucaine; D005456:Fluorescent Dyes; D007093:Imidazoles; D012996:Solutions; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils; C034384:coptisine; C005413:palmatine
keywords: Competitive interaction; Cucurbit[7]uril; Dibucaine; Spectrofluorometry; Supramolecular chemistry