title: Determination of ursolic and oleanolic acid in Sambuci fructus.
abstract: Elderberries are used in the preparation of pie, jelly, punch, wine, or liqueur, as well as in many herbal remedies and food supplements. Elderberry products may provide diaphoretic, diuretic, antioxidant, and immunostimulant activities that offer protection against cold and flu. Herein, we report for the first time the qualitative and quantitative evaluation of two isomeric triterpenoids isolated from Sambuci fructus. The analysis revealed that ursolic acid and oleanolic acid are present in Sambuci fructus. The average concentration of ursolic acid was ca. three times higher than the concentration of oleanolic acid. The triterpenoids were detected and quantified using chromatographic methods such as TLC and HPLC. Spectroscopic techniques, including HR-MS and 2D-NMR, allowed unequivocal structure determination.
authors.affiliation: Department of Pharmacognosy, Wroclaw Medical University, Borowska 211 A, PL-50-556 Wrocław, (phone/fax: +48-71-7840218). michal.glensk@umed.wroc.pl.
authors.forename: Michał
authors.initials: M
authors.lastname: Gleńsk
authors.forename: Jan A
authors.initials: JA
authors.lastname: Gliński
authors.forename: Maciej
authors.initials: M
authors.lastname: Włodarczyk
authors.forename: Piotr
authors.initials: P
authors.lastname: Stefanowicz
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D013058:Mass Spectrometry; D009828:Oleanolic Acid / Q000032:analysis*; D066244:Proton Magnetic Resonance Spectroscopy; D027923:Sambucus / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid
keywords: Elderberries; Oleanolic acid; Sambuci fructus; Triterpenes; Ursolic acid