title: Evaluation of pooled ocular and vaginal swabs by the Cepheid GeneXpert CT/NG assay for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae compared to the GenProbe Aptima Combo 2 Assay.
abstract: Ocular swabs from Tanzania were tested for Chlamydia trachomatis (CT), and self-collected vaginal swabs collected through a home collection program, iwantthekit.org, were tested for Neisseria gonorrhoeae and CT to evaluate Cepheid GeneXpert for the use of pooling multiple specimens before testing. GeneXpert shows to be a promising test for pooling.
authors.affiliation: International Sexually Transmitted Diseases Research Laboratory, Division of Infectious Diseases, Johns Hopkins University, Baltimore, MD, USA. Electronic address: Ldize2@jhmi.edu.
authors.forename: L
authors.initials: L
authors.lastname: Dize
authors.affiliation: Dana Center for Preventive Ophthalmology, Wilmer Eye Institute, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: West
authors.affiliation: Kongwa Trachoma Project, Tanzania.
authors.forename: H
authors.initials: H
authors.lastname: Mkocha
authors.affiliation: International Sexually Transmitted Diseases Research Laboratory, Division of Infectious Diseases, Johns Hopkins University, Baltimore, MD, USA; Division of Intramural Research, National Institute for Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Quinn
authors.affiliation: International Sexually Transmitted Diseases Research Laboratory, Division of Infectious Diseases, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Gaydos
mesh_terms: D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005123:Eye / Q000382:microbiology; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis*; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D013636:Tanzania; D014621:Vagina / Q000382:microbiology
keywords: Chlamydia trachomatis; Neisseria gonorrhoeae; Pooling; Trachoma