title: Improving quantification using curtain flow chromatography columns in the analysis of labile compounds: a study on amino acids.
abstract: The performance of curtain flow chromatography column technology with MS detection was evaluated for the analysis of labile compounds. The curtain flow column design allows for separations that are faster and/or more sensitive than conventional columns, depending on how exactly the curtain flow column is configured. For example, when mass spectral detection is employed, the curtain flow column can yield separations that are 5-times faster than conventional columns when the curtain flow and the conventional columns have the same internal diameter. Or when the internal diameter of the conventional column is reduced in order to yield the same analytical through-put as the curtain flow column, the sensitivity on the curtain flow column can be as much as 66-fold higher than the conventional column. As a consequence of the higher analytical through-put less standardization is required in the analysis of labile compounds because less sample degradation is apparent. Consequently the sample integrity is preserved yielding data of a higher quality.
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW, Australia.
authors.forename: D
authors.initials: D
authors.lastname: Kocic
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: L
authors.initials: L
authors.lastname: Pereira
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: T
authors.initials: T
authors.lastname: Edge
authors.affiliation: Thermo Fisher Scientific, Manor Park, Tudor Road, Runcorn, UK.
authors.forename: H
authors.initials: H
authors.lastname: Ritchie
authors.affiliation: Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia.
authors.forename: X A
authors.initials: XA
authors.lastname: Conlan
authors.affiliation: Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, University of Western Sydney (Parramatta), Sydney, NSW, Australia. Electronic address: r.shalliker@uws.edu.au.
authors.forename: R A
authors.initials: RA
authors.lastname: Shalliker
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*
chemical_list: D000596:Amino Acids
keywords: Active flow technology; Amino acids; Curtain flow; HPLC–MS/MS; Labile compounds; Mass spectrometry