title: Crystallization and preliminary X-ray diffraction analysis of UspE from Escherichia coli.
abstract: Universal stress proteins (Usps) are among the most highly induced genes when bacteria are subjected to several stress conditions such as heat shock, nutrient starvation or the presence of oxidants or other stress agents. Escherichia coli has five small Usps and one tandem-type Usp. UspE (or YdaA) is the tandem-type Usp and consists of two Usp domains arranged in tandem. To date, the structure of UspE remains to be elucidated. To contribute to the molecular understanding of the function of the tandem-type UspE, UspE from E. coli was overexpressed and the recombinant protein was purified using Ni-NTA affinity, Q anion-exchange and gel-filtration chromatography. Crystals of UspE were obtained by sitting-drop vapour diffusion. A diffraction data set was collected to a resolution of 3.2 Å from flash-cooled crystals. The crystals belonged to the tetragonal space group I4122 or I4322, with unit-cell parameters a = b = 121.1, c = 241.7 Å.
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Chun Shan
authors.initials: CS
authors.lastname: Quan
authors.affiliation: College of Engineering, Yanbian University, Jilin Yanji 133002, People's Republic of China.
authors.forename: Xuanzhen
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, People's Republic of China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Jin
authors.affiliation: School of Physics and Materials Engineering, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Dedi
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Bioengineering, College of Life Science, Dalian Nationalities University, Dalian 116600, People's Republic of China.
authors.forename: Shengdi
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Nam Chul
authors.initials: NC
authors.lastname: Ha
mesh_terms: D001483:Base Sequence; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins
keywords: Usp domain; tandem-type UspE; universal stress proteins