title: Proteomic analysis of the extracellular matrix in idiopathic pes equinovarus.
abstract: Idiopathic pes equinovarus is a congenital deformity of the foot and lower leg defined as a fixation of the foot in adduction, supination, and varus. Although the pathogenesis of clubfoot remains unclear, it has been suggested that fibroblasts and growth factors are involved. To directly analyze the protein composition of the extracellular matrix in contracted tissue of patients with clubfoot. A total of 13 infants with idiopathic clubfoot treated with the Ponseti method were included in the present study. Tissue samples were obtained from patients undergoing surgery for relapsed clubfeet. Contracted tissues were obtained from the medial aspect of the talonavicular joint. Protein was extracted after digestion and delipidation using zip-tip C18. Individual collagenous fractions were detected using a chemiluminescent assay. Amino acid analysis of tissue samples revealed a predominance of collagens, namely collagen types I, III, and VI. The high content of glycine and h-proline suggests a predominance of collagens I and III. A total of 19 extracellular matrix proteins were identified. The major result of the present study was the observation that the extracellular matrix in clubfoot is composed of an additional 16 proteins, including collagens V, VI, and XII, as well as the previously described collagen types I and III and transforming growth factor β. The characterization of the general protein composition of the extracellular matrix in various regions of clubfoot may help in understanding the pathogenesis of this anomaly and, thus, contribute to the development of more efficacious therapeutic approaches.
authors.affiliation: Department of Orthopaedics, 1st Faculty of Medicine, University Hospital Bulovka, Charles University, Budínova 2, 180 00, Prague 8, Czech Republic, martinostadal@yahoo.com.
authors.forename: Martin
authors.initials: M
authors.lastname: Ošt'ádal
authors.forename: Adam
authors.initials: A
authors.lastname: Eckhardt
authors.forename: Jan
authors.initials: J
authors.lastname: Herget
authors.forename: Ivan
authors.initials: I
authors.lastname: Mikšík
authors.forename: Pavel
authors.initials: P
authors.lastname: Dungl
authors.forename: Jiří
authors.initials: J
authors.lastname: Chomiak
authors.forename: Monika
authors.initials: M
authors.lastname: Frydrychová
authors.forename: Michal
authors.initials: M
authors.lastname: Burian
mesh_terms: D000596:Amino Acids / Q000032:analysis; D003025:Clubfoot / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D040901:Proteomics / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000596:Amino Acids; D016326:Extracellular Matrix Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen