title: [Detection of HLA antibodies by Luminex and kidney transplantation decision making: a case report].
abstract: We report the case of a patient planned for living-related donor kidney transplantation. HLA antibodies research, performed by the Luminex method, is positive for all the available sera. Cross match was performed by complement-dependent lymphocytotoxicity technique with three family members. The first donor, her husband, was excluded due to the positivity of the cross-match and the presence of donor-specific anti HLA antibody (DSA). The second one, her daughter, was immediately excluded because the recipient also presented DSA. Despite the negativity of the cross match, the decision to transplant the patient with the third donor, her son, was difficult to take because of the presence of DSA identified by the Luminex method. Consequently, the patient could not be transplanted.
authors.affiliation: Unité d'histocompatibilité, Service de transfusion sanguine et d'hémovigilance, Centre hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Ouafa
authors.initials: O
authors.lastname: Atouf
authors.affiliation: Unité d'histocompatibilité, Service de transfusion sanguine et d'hémovigilance, Centre hospitalier Ibn Sina, Rabat, Maroc.
authors.forename: Chehrazade
authors.initials: C
authors.lastname: Brick
authors.affiliation: Unité d'histocompatibilité, Service de transfusion sanguine et d'hémovigilance, Centre hospitalier Ibn Sina, Rabat, Maroc, UPR d'immunologie, Faculté de médecine et de pharmacie de Rabat, Université Mohamed V, Maroc.
authors.forename: Malika
authors.initials: M
authors.lastname: Essakalli
mesh_terms: D000906:Antibodies / Q000032:analysis* / Q000097:blood; D000075202:Contraindications; D003657:Decision Making; D005260:Female; D006084:Graft Rejection / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing / Q000295:instrumentation / Q000379:methods; D006801:Humans; D016030:Kidney Transplantation; D008875:Middle Aged; D051437:Renal Insufficiency / Q000276:immunology / Q000628:therapy; D014019:Tissue Donors
chemical_list: D000906:Antibodies; D006680:HLA Antigens
keywords: Luminex; donor-specific anti HLA antibody; kidney transplantation