title: In vitro mutual interaction between tenocytes and adipose-derived mesenchymal stromal cells.
abstract: OBJECTIVE
  Tendon is a complex tissue with a reduced regenerative ability. Nowadays, little or nothing is known about the regenerative effect of adipose-derived mesenchymal stromal cells (ADSCs) on tendons.
  
  
  METHODS
  The study aimed to evaluate the in vitro mutual interaction of ADSCs and tenocytes in standard culture conditions and a microwound healing model. Tenocyte viability, microwound recovery and the expression of genes encoding for the main extracellular matrix components and ADSC viability, differentiation and growth factor gene expression were evaluated.
  
  
  RESULTS
  The effects of ADSCs on tenocytes were observed more in the microwound healing model, in which the rate of microwound healing and the expression of decorin, tenascin and collagens were significantly increased. The influence of tenocytes on ADSCs was also found in standard culture conditions: ADSCs were directed toward a tenogenic lineage, and growth factor expression increased.
  
  
  CONCLUSIONS
  This study clarifies some aspects of the mutual interaction of ADSCs and tenocytes and provides in vitro evidence for a possible future application of ADSCs as a therapeutic strategy for tendon repair.
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy. Electronic address: francesca.veronesi@ior.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Veronesi
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory of Biocompatibility, Innovative Technologies and Advanced Therapies, Department Rizzoli RIT, Bologna, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Torricelli
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy; Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Della Bella
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory of Biocompatibility, Innovative Technologies and Advanced Therapies, Department Rizzoli RIT, Bologna, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Pagani
authors.affiliation: Laboratory of Preclinical and Surgical Studies, Rizzoli Orthopedic Institute, Bologna, Italy; Laboratory of Biocompatibility, Innovative Technologies and Advanced Therapies, Department Rizzoli RIT, Bologna, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Fini
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002450:Cell Communication; D002454:Cell Differentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D003094:Collagen / Q000378:metabolism; D058575:Decorin / Q000378:metabolism; D005109:Extracellular Matrix; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019063:Tenascin / Q000378:metabolism; D013708:Tendon Injuries / Q000628:therapy*; D013710:Tendons / Q000166:cytology / Q000502:physiology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: C546878:DCN protein, human; D058575:Decorin; D019063:Tenascin; D003094:Collagen
keywords: adipose-derived mesenchymal stromal cells; gene expression; mutual interaction; tendon healing; tenocytes