title: Inactivation kinetics of foodborne pathogens by UV-C radiation and its subsequent growth in fresh-cut kailan-hybrid broccoli.
abstract: The inactivation of Escherichia coli, S. Enteritidis and Listeria monocytogenes after UV-C radiation with 0, 2.5, 5, 7.5, 10 and 15 kJ UV-C m(-2) on fresh-cut kailan-hybrid broccoli was explored. Inactivation did not follow linear kinetics. Hence, it was modelled by using the Weibull distribution function, obtaining adjusted R(2) values higher than 94%, indicative of the accuracy of the model to the experimental data. The UV-C doses needed to reduce 1 log cycle the E. coli, S. Enteritidis and L. monocytogenes counts were 1.07, 0.02 and 9.26 kJ m(-2), respectively, being S. Enteritidis the most sensitive microorganism to UV-C radiation while L. monocytogenes was the most resistant. According to experimental data, UV-C doses higher than 2.5 kJ m(-2) did not achieve great microbial reductions. No differences in the growth behaviour of these microorganisms was observed in the treated samples stored under air conditions at 5, 10 and 15 °C, compared to the control. Conclusively, low UV-C doses are effective to reduce E. coli, S. Enteritidis and L. monocytogenes populations in fresh-cut kailan-hybrid broccoli keeping such counts stable during shelf life at 5-10 °C. The current study provides inactivation models for these foodborne pathogens that can be used in microbial risk assessment.
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain.
authors.forename: Ginés Benito
authors.initials: GB
authors.lastname: Martínez-Hernández
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain.
authors.forename: Juan-Pablo
authors.initials: JP
authors.lastname: Huertas
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Navarro-Rico
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain.
authors.forename: Perla A
authors.initials: PA
authors.lastname: Gómez
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain; Department of Food Engineering, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203, Cartagena, Murcia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Artés
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain; Department of Food Engineering, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203, Cartagena, Murcia, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Palop
authors.affiliation: Institute of Plant Biotechnology, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Campus Muralla del Mar s/n, 30202, Cartagena, Murcia, Spain; Department of Food Engineering, Campus de Excelencia Internacional Regional 'Campus Mare Nostrum', Universidad Politécnica de Cartagena, Paseo Alfonso XIII, 48, 30203, Cartagena, Murcia, Spain. Electronic address: fr.artes-hdez@upct.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Artés-Hernández
mesh_terms: D001937:Brassica / Q000382:microbiology*; D004926:Escherichia coli / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D005514:Food Irradiation / Q000379:methods*; D007700:Kinetics; D008089:Listeria monocytogenes / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D012477:Salmonella enteritidis / Q000737:chemistry / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays
keywords: Brassica oleracea Italica × Alboglabra group; Food safety; Frequency distributions; Microbial inactivation; Minimally processed; Weibull model