title: Genome-wide determination of poly(A) site choice in plants.
abstract: Second generation DNA sequencing technologies have been a great boon for the study of mRNA polyadenylation. The experimental determination of large numbers of polyadenylation sites using high-throughput sequencing strategies has provided the necessary platform for deeper understanding the regulation of gene expression in eukaryotes. For generating large sets of data to map poly-A sites, specialized sample preparations that target the junction of 3'-UTR and the poly(A) tail are usually employed. Here, we describe three different protocols that are effectively used for global determinations of poly(A) site choice in plants.
authors.affiliation: Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
authors.forename: Pratap Kumar
authors.initials: PK
authors.lastname: Pati
authors.forename: Liuyin
authors.initials: L
authors.lastname: Ma
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Hunt
mesh_terms: D020413:3' Untranslated Regions; D015870:Gene Expression; D018745:Genome, Plant; D010944:Plants / Q000235:genetics / Q000378:metabolism*; D011061:Poly A / Q000235:genetics / Q000378:metabolism*; D026723:Polyadenylation; D012333:RNA, Messenger / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D012333:RNA, Messenger; D018749:RNA, Plant; D011061:Poly A