title: Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers.
abstract: Mass spectrometry-based methods for prostate cancer biomarker discovery are hampered by their low-throughput capabilities because of extensive sample preparation. We present the parafilm-assisted microdissection technique coupled with label-free quantification and bioinformatics analysis as a means to evaluate directly protein expression changes on benign and tumor regions.
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de masse, INSERM, Université de Lille 1, Bât. SN3, 1er étage, F-59655 Villeneuve D'Ascq, France. isabelle.fournier@univ-lille1.fr.
authors.forename: J
authors.initials: J
authors.lastname: Quanico
authors.forename: J
authors.initials: J
authors.lastname: Franck
authors.forename: J P
authors.initials: JP
authors.lastname: Gimeno
authors.forename: R
authors.initials: R
authors.lastname: Sabbagh
authors.forename: M
authors.initials: M
authors.lastname: Salzet
authors.forename: R
authors.initials: R
authors.lastname: Day
authors.forename: I
authors.initials: I
authors.lastname: Fournier
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000096:biosynthesis; D019295:Computational Biology; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D042282:Microdissection / Q000379:methods*; D009363:Neoplasm Proteins / Q000032:analysis* / Q000096:biosynthesis; D010232:Paraffin; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D010232:Paraffin