title: MicroRNA-196b promotes cell proliferation and suppress cell differentiation in vitro.
abstract: MicroRNA-196b (miR-196b) is frequently amplified and aberrantly overexpressed in acute leukemias. To investigate the role of miR-196b in acute leukemias, it has been observed that forced expression of this miRNA increases proliferation and inhibits apoptosis in human cell lines. More importantly, we show that this miRNA can significantly increase the colony-forming capacity of mouse normal bone marrow progenitor cells alone, as well as partially blocking the cells from differentiation. Taken together, our studies suggest that miRNA-196b may play an essential role in the development of MLL-associated leukemias through inhibiting cell differentiation and apoptosis, while promoting cell proliferation.
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China. Electronic address: caodlgz@sina.com.
authors.forename: Donglin
authors.initials: D
authors.lastname: Cao
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Liangshan
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Da
authors.initials: D
authors.lastname: Lei
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Maorui
authors.initials: M
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Huawen
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, Guangdong No. 2 Provincial People's Hospital, Guangzhou 510317, PR China.
authors.forename: Limei
authors.initials: L
authors.lastname: Zhong
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D005786:Gene Expression Regulation; D006801:Humans; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: Cell apoptosis; Colony-forming/replating assay; Differentiation; Proliferation; Viability; miR-196b