title: Protective effects of green tea polyphenol against cisplatin-induced nephrotoxicity in rats.
abstract: OBJECTIVE
  This study is to compare the effects of green tea polyphenol (GTP) pre-treatment with those of GTP post-treatment on cisplatin (CP)-induced nephrotoxicity in rat.
  
  
  METHODS
  Male Sprague-Dawley rats were randomly divided into six groups. Animals in the control group received 0.9% saline (intraperitoneal); animals in the GTP group received 0.9% saline and GTP (0.2% GTP as their sole source of drinking water); the CP group received only CP (7 mg/kg, intraperitoneal); the CP+preGTP group received GTP from two days before CP to four days after CP and the CP+postGTP group received GTP for four days after CP. CP-induced renal toxicity was evaluated by plasma creatinine and blood urea nitrogen (BUN) concentrations; kidney tissue γ-glutamyl transpeptidase (GGT) and alkaline phosphatase (AP) activities and histopathological examinations.
  
  
  RESULTS
  High serume creatinine and BUN concentrations were observed in CP treated rats. The GGT and AP activites were lower in kidney of CP treated rats compared to control rats. In addition, treatment with CP resulted in development of a marked tubular necrosis, and tubular dilation in kidney of rats. Pretreatment with GTP resulted in markedly reduced elevation of serum creatinine and BUN amounts and changes of GGT and AP activity in kidney induced by CP. CP-induced histopathological changes, including tubular necrosis and dilation, were ameliorated in GTP pre-treated rats, compared to CP alone or GTP post-treated rats.
  
  
  CONCLUSIONS
  These results demonstrate that GTP might have some protective effect against CP-induced nephrotoxicity in rat, and GTP pre-treatment was more effective than GTP post-treatment on reduction of CP-induced renal dysfunction.
authors.affiliation: Department of Obstetrics and Gynecology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Tae-Gyu
authors.initials: TG
authors.lastname: Ahn
authors.affiliation: Department of Obstetrics and Gynecology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Han-Kyoung
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: So-Won
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Soo-Ah
authors.initials: SA
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Molecular Biology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Byoung-Rai
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Chosun University College of Medicine, Gwangju, Korea.
authors.forename: Sei Jun
authors.initials: SJ
authors.lastname: Han
keywords: Cisplatin; Green tea polyphenol; Nephrotoxicity