title: A multivariate analysis using physiology and behavior to characterize robustness in two isogenic lines of rainbow trout exposed to a confinement stress.
abstract: Robustness is a complex trait difficult to characterize and phenotype. In the present study, two features of robustness in rainbow trout were investigated: sensitivity and resilience to an acute stressor. For that purpose, oxygen consumption, cortisol release, group dispersion and group activity of two isogenic lines of juvenile rainbow trout were followed before and after an environmental challenge. The effect of a 4h confinement protocol (~140kg/m(3)), which is generally considered as a highly stressful challenge, was investigated. Temporal patterns produced by this experiment were analyzed using multivariate statistics on curve characteristics to describe physiological and behavioral adaptive systems for each isogenic line. The two isogenic lines were found to be highly divergent in their corticosteroid reactivity. However, no correlation between physiological and behavioral sensitivity or resilience was observed. Furthermore, the multivariate analysis results indicated two separate and independent fish group coping strategies, i.e. by favoring either behavioral or physiological responses. In addition, considerable intra-line variabilities were observed, suggesting the importance of micro-environment effects on perturbation sensitivities. In this context, cortisol release rate variability was found to be related to the pre-stress social environment, with a strong correlation between pre-stress aggressiveness and cortisol release rate amplitude. Overall, this approach allowed us to extract important characteristics from dynamic data in physiology and behavior to describe components of robustness in two isogenic lines of rainbow trout.
authors.affiliation: INRA, LPGP Fish Physiology and Genomics UR1037, Rennes, France. Electronic address: bsadoul@rennes.inra.fr.
authors.forename: Bastien
authors.initials: B
authors.lastname: Sadoul
authors.affiliation: INRA, LPGP Fish Physiology and Genomics UR1037, Rennes, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Leguen
authors.affiliation: INRA, LPGP Fish Physiology and Genomics UR1037, Rennes, France.
authors.forename: Violaine
authors.initials: V
authors.lastname: Colson
authors.affiliation: INRA, Modélisation Systémique Appliquée aux Ruminants UMR0791, Paris, France; AgroParisTech, Modélisation Systémique Appliquée aux Ruminants UMR0791, Paris, France.
authors.forename: Nicolas C
authors.initials: NC
authors.lastname: Friggens
authors.affiliation: INRA, LPGP Fish Physiology and Genomics UR1037, Rennes, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Prunet
mesh_terms: D000223:Adaptation, Psychological; D000374:Aggression / Q000502:physiology; D000818:Animals; D006854:Hydrocortisone / Q000378:metabolism; D015999:Multivariate Analysis; D017686:Oncorhynchus mykiss / Q000502:physiology*; D010101:Oxygen Consumption; D012149:Restraint, Physical; D013223:Statistics as Topic; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D006854:Hydrocortisone
keywords: Adaptation; Aggressiveness; Cortisol; Stress