title: Theranostic vitamin E TPGS micelles of transferrin conjugation for targeted co-delivery of docetaxel and ultra bright gold nanoclusters.
abstract: The aim of this work was to develop an advanced theranostic micelles of D-alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS), which are conjugated with transferrin for targeted co-delivery of docetaxel (DTX) as a model drug and ultra bright gold clusters (AuNC) as a model imaging agent for simultaneous cancer imaging and therapy. The theranostic micelles with and without transferrin conjugation were prepared by the solvent casting method and characterized for their particle size, polydispersity, surface chemistry, drug encapsulation efficiency, drug loading and cellular uptake efficiency. Transferrin receptors expressing MDA-MB-231-luc breast cancer cells and NIH-3T3 fibroblast cells (control cells without transferrin receptor expression) were employed as an in vitro model to access cytotoxicity of the formulations. The overexpression of transferrin receptor on the surface of MDA-MB-231-luc cells was confirmed by flow cytometry. The biodistribution study and theranostic efficacy of the micelles were investigated by using the Xenogen IVIS(®) Spectrum imaging system, which includes AuNC based fluorescence imaging and luciferase induced bioluminescence imaging on MDA-MB-231-luc tumor bearing SCID mice. The IC50 values demonstrated that the non-targeted and targeted micelles could be 15.31 and 71.73 folds more effective than Taxotere(®) after 24 h treatment with the MDA-MB-231-luc cells. Transferrin receptor targeted delivery of such micelles was imaged in xenograft model and showed their great advantages for real-time tumor imaging and inhibition of tumor growth.
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore; Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Madaswamy S
authors.initials: MS
authors.lastname: Muthu
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore.
authors.forename: Rajaletchumy Veloo
authors.initials: RV
authors.lastname: Kutty
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore.
authors.forename: Zhentao
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore; International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, Shanghai 200433, China. Electronic address: chefss@nus.edu.sg.
authors.forename: Si-Shen
authors.initials: SS
authors.lastname: Feng
mesh_terms: D000818:Animals; D001943:Breast Neoplasms; D002460:Cell Line; D000077143:Docetaxel; D004337:Drug Carriers / Q000737:chemistry*; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D016513:Mice, SCID; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D041681:NIH 3T3 Cells; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D043823:Taxoids / Q000008:administration & dosage* / Q000737:chemistry*; D014168:Transferrin / Q000737:chemistry*; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D008823:Micelles; D043823:Taxoids; D014168:Transferrin; D014810:Vitamin E; D000077143:Docetaxel; D011092:Polyethylene Glycols; D006046:Gold; C014225:tocophersolan
keywords: Cancer nanotechnology; Diagnostic imaging; Gold nanoclusters; Molecular biomaterials; Nanomedicine; Nanotheranostics