title: Nanotemplated polyelectrolyte films as porous biomolecular delivery systems. Application to the growth factor BMP-2.
abstract: Biomaterials capable of delivering controlled quantities of bioactive agents, while maintaining mechanical integrity, are needed for a variety of cell contacting applications. We describe here a nanotemplating strategy toward porous, polyelectrolyte-based thin films capable of controlled biomolecular loading and release. Films are formed via the layer-by-layer assembly of charged polymers and nanoparticles (NP), then chemically cross-linked to increase mechanical rigidity and stability, and finally exposed to tetrahydrofuran to dissolve the NP and create an intra-film porous network. We report here on the loading and release of the growth factor bone morphogenetic protein 2 (BMP-2), and the influence of BMP-2 loaded films on contacting murine C2C12 myoblasts. We observe nanotemplating to enable stable BMP-2 loading throughout the thickness of the film, and find the nanotemplated film to exhibit comparable cell adhesion, and enhanced cell differentiation, compared with a non-porous cross-linked film (where BMP-2 loading is mainly confined to the film surface).
authors.affiliation: a Equipe de Recherche sur les Relations Matrice Extracellulaire Cellules; Institut des Matériaux; Université de Cergy-Pontoise; Cergy-Pontoise, France.
authors.forename: Adeline
authors.initials: A
authors.lastname: Gand
authors.forename: Mathilde
authors.initials: M
authors.lastname: Hindié
authors.forename: Diane
authors.initials: D
authors.lastname: Chacon
authors.forename: Paul R
authors.initials: PR
authors.lastname: Van Tassel
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D004573:Electrolytes / Q000737:chemistry*; D051379:Mice; D032446:Myoblasts / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D004573:Electrolytes; D011108:Polymers
keywords: BMP-2; biomaterial; cell differentiation; delivery system; layer-by-layer; polyelectrolyte; porous material