title: Effect of silk fibroin nanofibers containing silver sulfadiazine on wound healing.
abstract: BACKGROUND
  One of the promising applications of silk fibroin (SF) in biomedical engineering is its use as a scaffolding material for skin regeneration. The purpose of this study was to determine the wound healing effect of SF nanofibrous matrices containing silver sulfadiazine (SSD) wound dressings.
  
  
  METHODS
  An SF nanofibrous matrix containing SSD was prepared by electrospinning. The cell attachment and spreading of normal human epidermal keratinocytes (NHEK) and normal human epidermal fibroblasts (NHEF) to SF nanofibers containing three different concentrations of SSD contents (0.1, 0.5, and 1.0 wt%) were determined. In addition, a rat wound model was used in this study to determine the wound healing effect of SF nanofibers containing SSD compared with that of Acticoat™, a commercially available wound dressing.
  
  
  RESULTS
  The number of NHEK and NHEF attached to SF nanofibers containing SSD decreased when the concentration of SSD increased. The number of attached NHEF cells was lower than that of attached NHEK cells. The SF matrix with 1.0 wt% SSD produced faster wound healing than Acticoat, although 1.0 wt% SSD inhibited the attachment of epidermal cells to SF nanofibers in vitro.
  
  
  CONCLUSIONS
  The cytotoxic effects of SF nanofibers with SSD should be considered in the development of silver-release dressings for wound healing through its antimicrobial activity. It is challenging to design wound dressings that maximize antimicrobial activity and minimize cellular toxicity.
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, Seoul, South Korea.
authors.forename: Lim
authors.initials: L
authors.lastname: Jeong
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, Seoul, South Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Chungnam National University, Daejeon, Seoul, South Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Jung
authors.affiliation: Department of Oral Biochemistry, School of Dentistry, Seoul National University, Seoul, South Korea.
authors.forename: Byung Moo
authors.initials: BM
authors.lastname: Min
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, Seoul, South Korea.
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Park
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012837:Silver Sulfadiazine / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000293:injuries; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D005349:Fibroins; D012837:Silver Sulfadiazine
keywords: nanofibrous matrix; silk fibroin; silver sulfadiazine; wound healing