title: Controlling attachment and growth of Listeria monocytogenes in polyvinyl chloride model floor drains using a peroxide chemical, chitosan-arginine, or heat.
abstract: Listeria monocytogenes can colonize a poultry processing plant as a resident in floor drains. Limiting growth and attachment to drain surfaces may help lessen the potential for cross-contamination of product. The objective of this study was to compare a hydrogen peroxide-peroxyacetic acid-based chemical to chitosan-arginine or heat to prevent attachment of or destroy existing L. monocytogenes on the inner surface of model floor drains. L. monocytogenes was introduced to result in about 10(9) planktonic and attached cells within untreated polyvinyl chloride model drain pipes. Treatments (0.13 % peroxide-based sanitizer, 0.1 % chitosan-arginine, or 15 s of hot water at 95 to 100°C) were applied immediately after inoculation or after 24 h of incubation. Following treatment, all pipes were incubated for an additional 24 h; planktonic and attached cells were enumerated by plate count. All treatments significantly (P < 0.05) lowered numbers of planktonic and attached cells recovered. Chitosan-arginine resulted in approximately a 6-log reduction in planktonic cells when applied prior to incubation and a 3-log reduction after the inoculum had a chance to grow. Both heat and peroxide significantly outperformed chitosan-arginine (8- to 9-log reduction) and were equally effective before and after incubation. Heat was the only treatment that eliminated planktonic L. monocytogenes. All treatments were less effective against attached cells. Chitosan-arginine provided about a 4.5-log decrease in attached cells when applied before incubation and no significant decrease when applied after growth. Like with planktonic cells, peroxide-peroxyacetic acid and heat were equally effective before or after incubation, causing decreases ranging from 7 to 8.5 log for attached L. monocytogenes. Applied at the most efficacious time, any of these techniques may lessen the potential for L. monocytogenes to remain as a long-term resident in processing plant floor drains.
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Russell Research Center, Athens, Georgia 30605, USA. mark.berrang@ars.usda.gov.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Berrang
authors.affiliation: Department of Population Health, University of Georgia, Athens, Georgia 30605, USA.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Hofacre
authors.affiliation: Department of Food Science and Technology, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Frank
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology*; D048271:Chitosan / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D005430:Floors and Floorcoverings; D005525:Food-Processing Industry; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects*; D008460:Meat / Q000382:microbiology; D011143:Polyvinyl Chloride; D011200:Poultry
chemical_list: D004202:Disinfectants; D011143:Polyvinyl Chloride; D048271:Chitosan; D001120:Arginine; D006861:Hydrogen Peroxide