title: Simultaneous determination of blockers and agonists by on-fiber derivatization in self-made solid-phase microextraction coating fiber.
abstract: An environmentally friendly and sensitive method for determination of blockers and agonists was described in this paper. The method is based on a homemade sol-gel solid-phase microextraction (SPME) coating with simultaneous on-fiber derivatization and subsequent gas chromatography mass spectrometry (GC/MS) analysis. The influences of the main factors on the type and thickness of the homemade fiber coatings, conditions of the derivatization, extraction and desorption of SPME were investigated in detail. The proposed procedure showed limits of detection lower than 0.2 ng mL(-1). The linearity was in the 0.5-150 ng mL(-1) for clenbuterol and 1.0-100 ng mL(-1) for metoprolol and propranolol. The variation in measurements (repeatability) was below 8.7% (n=6) and the degree of difference between (reproducibility) was below 11.4% (n=3). In the application, spiked human saliva samples and real human saliva samples were analyzed, it was found that saliva would affect the detection of propranolol when it was a very low content. The established method can be feasible in practical application and helpful for agonists and blockers preliminary screening during the competition.
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China; Test Center of Fuzhou University, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China.
authors.forename: Xiayang
authors.initials: X
authors.lastname: Huang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China; Longyan Entry-Exit Inspection and Quarantine Bureau, Longyan, Fujian 364000, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China; Test Center of Fuzhou University, Fuzhou University, Fuzhou, Fujian 350002, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Lu
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China; Test Center of Fuzhou University, Fuzhou University, Fuzhou, Fujian 350002, China. Electronic address: zlan@fzu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000327:Adsorption; D002976:Clenbuterol / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D057230:Limit of Detection; D008790:Metoprolol / Q000032:analysis*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D011433:Propranolol / Q000032:analysis*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D000180:Acrylic Resins; D012833:Siloxanes; C402373:poly(methylhydrosiloxane); D011092:Polyethylene Glycols; C006903:carbopol 940; D011433:Propranolol; D008790:Metoprolol; D002976:Clenbuterol
keywords: Agonists; Blockers; Gas chromatography–mass spectrometry; On-fiber derivatization; Solid-phase microextraction