title: Association of nesfatin-1 level with body composition, dietary intake and resting metabolic rate in obese and morbid obese subjects.
abstract: OBJECTIVE
  Nesfatin-1 identified neuroendocrine peptide is involved in regulation of homeostasis via modulation of metabolism, energy homeostasis and food intake. We aimed to investigate the associations of circulating nesfatin-1 level with food intake, body composition and resting metabolic rate (RMR) and also examine the correlation between circulating peroxisome proliferator-activated receptor gamma (PPARγ) and nesfatin-1 levels in obese and morbid obese subjects.
  
  
  METHODS
  A total of 96 obese subjects (including 18 morbid obese subjects) were participated in the current cross-sectional study. We assessed the body composition with the use of Body Composition Analyzer. RMR was measured by means of the MetaCheck™, an instrument designed to measure RMR using indirect calorimetry. All baseline blood samples were obtained following an overnight fasting. Plasma concentrations of nesfatin-1 and circulating PPARγ were measured with the use of an ELISA method. Statistical analyses were performed using SPSS.
  
  
  RESULTS
  We found significant associations between fat percent and circulating nesfatin-1 in obese and morbid obese subjects. There was main association between circulating nesfatin-1 and PPARγ concentration in obese subjects and it was more strong association in morbid obese participants. There was marginally significant differences between percent predicted RMR between different categorized nesfatin-1 levels. There were also higher intakes of calorie, carbohydrate and protein in obese group who had lower concentration of nesfatin-1.
  
  
  CONCLUSIONS
  Our data indicated the fat percent as main determinant factor in circulating nesfatin-1 level. It appears nesfatin-1 and PPARγ might be concurrently involved in adipogenesis pathway.
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Mirzaei
authors.affiliation: Tehran University of Medical Sciences, Tehran, Iran; Department of Medicine, Section of Endocrinology, Nutrition, and Diabetes, Vitamin D, Skin and Bone Research Laboratory, Boston University Medical Center, Boston, MA, United States.
authors.forename: Arash
authors.initials: A
authors.lastname: Hossein-nezhad
authors.affiliation: Clinical Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Keshavarz
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Koohdani
authors.affiliation: Department of Statistics, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Eshraghian
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboor-Yaraghi
authors.affiliation: Clinical Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Hosseini
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Chamari
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Zareei
authors.affiliation: Cellular and Molecular Nutrition Department, School of Nutritional Science and Dietetics, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: jalalimahmoud@hotmail.com.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Djalali
mesh_terms: D000328:Adult; D001481:Basal Metabolism; D015415:Biomarkers / Q000097:blood*; D001823:Body Composition; D015992:Body Mass Index; D002135:Calcium-Binding Proteins / Q000097:blood*; D002153:Calorimetry, Indirect; D003430:Cross-Sectional Studies; D004268:DNA-Binding Proteins / Q000097:blood*; D002149:Energy Intake; D005215:Fasting; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D009419:Nerve Tissue Proteins / Q000097:blood*; D000080866:Nucleobindins; D009765:Obesity / Q000097:blood / Q000503:physiopathology*; D009767:Obesity, Morbid / Q000097:blood / Q000503:physiopathology*; D047495:PPAR gamma / Q000097:blood; D011379:Prognosis; D012307:Risk Factors
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; C000630650:NUCB2 protein, human; D009419:Nerve Tissue Proteins; D000080866:Nucleobindins; D047495:PPAR gamma
keywords: Dietary intake; Nesfatin-1; PPARγ, Obesity; Resting metabolic rate