title: CD152 and PD-1 down-regulation on CD8 T cells is associated with human acute liver allograft rejection.
abstract: OBJECTIVE
  To investigate CD152 and PD-1 expression on T lymphocytes and the function of CD152- and PD-1-positive CD8 T cells in human acute liver allograft rejection.
  
  
  METHODS
  Sixty-three patients undergoing liver transplantation were enrolled in this study, including 26 cases with acute allograft rejection (Gr-AR) and 37 cases with stable allograft liver function (Gr-SF). The expression of CD152 and PD-1 on T lymphocytes and the expression of granzyme and perforin on CD152- and PD-1-positive CD8 T cells in peripheral blood were analyzed using flow cytometry.
  
  
  RESULTS
  The peripheral CD4/CD8 ratio in Gr-AR was significantly lower than that in Gr-SF (P < .01). The expression of CD152 and PD-1 on CD8 and CD4 T cells was significantly lower in Gr-AR than in Gr-SF (all P < .01). The expression of granzyme B and perforin was significantly higher in Gr-AR than in Gr-SF (P < .01).
  
  
  CONCLUSIONS
  Down-regulation of the expression of negative costimulatory molecules such as CD152 and PD-1 on CD8 T cells may be associated with human acute liver allograft rejection.
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Peking University 302 Hospital, Beijing, China.
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.affiliation: Peking University 302 Hospital, Beijing, China.
authors.forename: W
authors.initials: W
authors.lastname: Ren
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: M
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: H o
authors.initials: Ho
authors.lastname: Wang
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Research Center for Biological Therapy, Beijing 302 Hospital, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China.
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.affiliation: Research Center for Liver Transplantation, Beijing 302 Hospital, Beijing, China. Electronic address: shiming302@sina.com.
authors.forename: M
authors.initials: M
authors.lastname: Shi
mesh_terms: D064591:Allografts; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D015536:Down-Regulation; D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D016031:Liver Transplantation; D014184:Transplantation, Homologous