title: Biosynthesis of silver nanoparticles using ethanolic petals extract of Rosa indica and characterization of its antibacterial, anticancer and anti-inflammatory activities.
abstract: The present study was aimed at biosynthesis of silver nanoparticles (AgNPs) using ethanolic extract of rose (Rosa indica) petals and testing their potential antibacterial activity using selective human pathogenic microbes, anticancer activity using human colon adenocarcinoma cancer cell line HCT 15 as well as anti-inflammatory activity using rat peritoneal macrophages in vitro. The biologically synthesized AgNPs were also characterized by UV-visible spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The characterized AgNPs showed an effective antibacterial activity against Gram negative (Escherichia coli, Klebsiella pneumoniae) than Gram positive (Streptococcus mutans, Enterococcus faecalis) bacteria. MTT assay, analysis of nuclear morphology, mRNA expression of Bcl-2, Bax and protein expression of caspase 3 as well as 9, indicated potential anticancer activity. In addition, green synthesized AgNPs also attenuated cytotoxicity, nuclear morphology and free radical generation (O2(-) and NO) by rat peritoneal macrophages in vitro. The results of our study show the potential green synthesis of silver nanoparticles in mitigating their toxicity while retaining their antibacterial activities.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: manikandanramar@yahoo.co.in.
authors.forename: Ramar
authors.initials: R
authors.lastname: Manikandan
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Beulaja
authors.initials: B
authors.lastname: Manikandan
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Thiagarajan
authors.initials: T
authors.lastname: Raman
authors.affiliation: Department of Biotechnology, Sri Sankara Arts and Science College, Enathur, Kanchipuram 631 561, India.
authors.forename: Koodalingam
authors.initials: K
authors.lastname: Arunagirinathan
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Narayanan Marimuthu
authors.initials: NM
authors.lastname: Prabhu
authors.affiliation: Department of Botany (DDE), Alagappa University, Karaikudi 630 003, India.
authors.forename: Muthuramalingam
authors.initials: M
authors.lastname: Jothi Basu
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Muthulakshmi
authors.initials: M
authors.lastname: Perumal
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Palanisamy
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: marumugam7@hotmail.com.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Munusamy
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000188:drug therapy; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D027826:Rosa / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D010936:Plant Extracts; D000431:Ethanol; D012834:Silver
keywords: Anti-inflammatory; Antibacterial; Anticancer; Rosa indica; Silver nanoparticles