title: Backbone resonance assignments of the 54 kDa dimeric C-terminal domain of murine STING in complex with DMXAA.
abstract: The mammalian ER protein STING (stimulators of interferon genes) is an important innate immunity protein for linking detection of novel secondary messengers c-di-GMP, c-di-AMP, cGAMP or cytosolic dsDNA to the activation of TANK kinase 1 and its downstream interferon regulator factor 3. Recently quite a few of crystal structures representing different states of the C-terminal domain (CTD) of human and murine STING (hSTING and mSTING) in complex with c-di-GMP, cGAMP or DMXAA have been reported. However, the C-terminal 42 residues of STING-CTD, which may be important in mediating the downstream reactions, is invisible or absent in all reported X-ray structures. In addition, X-ray crystal structures may be subject to crystal packing force. Hence an alternate method of determining the structure and function of STING in a near physiological condition is essential. We now report the near complete backbone resonance assignments of the 54 kDa dimeric mSTING-CTD in complex with DMXAA, which is the first step in determining its complex structure and understanding why DMXAA, which is a very efficient agent for curing mouse cancer, is totally ineffective in human.
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan, ROC.
authors.forename: Yuan-Chao
authors.initials: YC
authors.lastname: Lou
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan, ROC.
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Kao
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung, 40227, Taiwan, ROC.
authors.forename: Ko-Hsin
authors.initials: KH
authors.lastname: Chin
authors.affiliation: Institute of Biochemistry, National Chung Hsing University, Taichung, 40227, Taiwan, ROC.
authors.forename: Jen-Kang
authors.initials: JK
authors.lastname: Chen
authors.affiliation: Institute of Biochemistry, National Chung Hsing University, Taichung, 40227, Taiwan, ROC.
authors.forename: Je-Le
authors.initials: JL
authors.lastname: Tu
authors.affiliation: Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan, ROC. bmchinp@ibms.sinica.edu.tw.
authors.forename: Chinpan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Agricultural Biotechnology Center, National Chung Hsing University, Taichung, 40227, Taiwan, ROC. shchou@nchu.edu.tw.|Institute of Biochemistry, National Chung Hsing University, Taichung, 40227, Taiwan, ROC. shchou@nchu.edu.tw.
authors.forename: Shan-Ho
authors.initials: SH
authors.lastname: Chou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D008565:Membrane Proteins / Q000737:chemistry*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy; D044004:Xanthones / Q000737:chemistry*; D000099197:STING Protein
chemical_list: D008565:Membrane Proteins; D044004:Xanthones; C530855:Sting1 protein, mouse; D000099197:STING Protein; C066668:vadimezan
keywords: DMXAA; Innate immunity; STING; c-di-AMP; c-di-GMP; cGAMP