title: In situserial Laue diffraction on a microfluidic crystallization device.
abstract: Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform for serial Laue diffraction from macroscopic crystals and proposes that a collection of small slices of Laue data from many individual crystals is a realistic solution to the difficulties in dynamic studies of irreversible biochemical reactions.
authors.affiliation: Department of Chemical Engineering, The University of Massachusetts Amherst , Amherst, MA, USA ; Institute for Molecular Engineering, The University of Chicago , Chicago, IL, USA ; Department of Chemical and Biomolecular Engineering, The University of Illinois at Urbana-Champaign , Urbana, IL, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Perry
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Illinois at Urbana-Champaign , Urbana, IL, USA.
authors.forename: Sudipto
authors.initials: S
authors.lastname: Guha
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Illinois at Urbana-Champaign , Urbana, IL, USA.
authors.forename: Ashtamurthy S
authors.initials: AS
authors.lastname: Pawate
authors.affiliation: Center for Advanced Radiation Sources, The University of Chicago , Argonne, IL, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Henning
authors.affiliation: Center for Advanced Radiation Sources, The University of Chicago , Argonne, IL, USA.
authors.forename: Irina
authors.initials: I
authors.lastname: Kosheleva
authors.affiliation: Center for Advanced Radiation Sources, The University of Chicago , Argonne, IL, USA.
authors.forename: Vukica
authors.initials: V
authors.lastname: Srajer
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Illinois at Urbana-Champaign , Urbana, IL, USA.
authors.forename: Paul J A
authors.initials: PJ
authors.lastname: Kenis
authors.affiliation: Center for Advanced Radiation Sources, The University of Chicago , Argonne, IL, USA ; Renz Research Inc. , Westmont, IL, USA.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Ren
keywords: Laue diffraction; microfluidics; protein crystallization; serial crystallography