title: An updated insight into the Sialotranscriptome of Triatoma infestans: developmental stage and geographic variations.
abstract: BACKGROUND
  Triatoma infestans is the main vector of Chagas disease in South America. As in all hematophagous arthropods, its saliva contains a complex cocktail that assists blood feeding by preventing platelet aggregation and blood clotting and promoting vasodilation. These salivary components can be immunologically recognized by their vector's hosts and targeted with antibodies that might disrupt blood feeding. These antibodies can be used to detect vector exposure using immunoassays. Antibodies may also contribute to the fast evolution of the salivary cocktail.
  
  
  METHODS
  Salivary gland cDNA libraries from nymphal and adult T. infestans of breeding colonies originating from different locations (Argentina, Chile, Peru and Bolivia), and cDNA libraries originating from F1 populations of Bolivia, were sequenced using Illumina technology. Coding sequences (CDS) were extracted from the assembled reads, the numbers of reads mapped to these CDS, sequences were functionally annotated and polymorphisms determined.
  Over five thousand CDS, mostly full length or near full length, were publicly deposited on GenBank. Transcripts that were over 10-fold overexpressed from different geographical regions, or from different developmental stages were identified. Polymorphisms were mapped to derived coding sequences, and found to vary between developmental instars and geographic origin of the biological material. This expanded sialome database from T. infestans should be of assistance in future proteomic work attempting to identify salivary proteins that might be used as epidemiological markers of vector exposure, or proteins of pharmacological interest.
authors.affiliation: Institute of Parasitology, Biology Centre of the Academy of Sciences of Czech Republic, Ceske Budejovice, Czech Republic.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schwarz
authors.affiliation: Laboratory of Chagas Disease and Immunoparasitology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
authors.forename: Nora
authors.initials: N
authors.lastname: Medrano-Mercado
authors.affiliation: Zoology/Parasitology Group, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Günter A
authors.initials: GA
authors.lastname: Schaub
authors.affiliation: Escola Nacional de Saúde Pública. Fiocruz, Rio de Janeiro, Brazil.
authors.forename: Claudio J
authors.initials: CJ
authors.lastname: Struchiner
authors.affiliation: Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Valencia, Spain.
authors.forename: M Dolores
authors.initials: MD
authors.lastname: Bargues
authors.affiliation: Universidad Peruana Cayetano Heredia, Sede de Arequipa, Arequipa, Peru; Department of Biostatistics and Epidemiology, Center for Clinical Epidemiology and Biostatistics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
authors.forename: Michael Z
authors.initials: MZ
authors.lastname: Levy
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland, United States of America.
authors.forename: José M C
authors.initials: JM
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D015723:Gene Library; D012463:Saliva / Q000737:chemistry*; D012471:Salivary Proteins and Peptides / Q000235:genetics* / Q000378:metabolism; D013020:South America; D059467:Transcriptome / Q000235:genetics*; D014224:Triatoma / Q000235:genetics* / Q000378:metabolism
chemical_list: D012471:Salivary Proteins and Peptides