title: Heterogeneity of Bordetella bronchiseptica adenylate cyclase (cyaA) RTX domain.
abstract: Bordetella bronchiseptica is a widespread pathogen, with a broad host range, occasionally including humans. Diverse virulence factors (adhesins, toxins) allow its adaptation to its host, but this property of the adenylate cyclase (cyaA) toxin is not well understood. In this study, we analyzed the repeats-in-toxin domain of B. bronchiseptica cyaA with PCR, followed by restriction fragment length analysis. Of ninety-two B. bronchiseptica strains collected from different hosts and geographic regions, 72 (78.3 %) carried cyaA and four RFLP types (A-D) were established using NarI and SalI. However, in 20 strains, cyaA was replaced with a peptide transport protein operon. A phylogenetic tree based on partial nucleotide sequences of cyaA revealed that group 2 contains strains of specifically human origin, whereas subgroup 1a contains all but one of the strains from pigs. The human strains showed many PCR-RFLP and sequence variants, confirming the clonal population structure of B. bronchiseptica.
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, 1143, Budapest, Hungary, wehmann.eniko@agrar.mta.hu.
authors.forename: Enikő
authors.initials: E
authors.lastname: Wehmann
authors.forename: Bernadett
authors.initials: B
authors.lastname: Khayer
authors.forename: Tibor
authors.initials: T
authors.lastname: Magyar
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics; D037361:Adenylate Cyclase Toxin / Q000737:chemistry* / Q000235:genetics*; D018829:Adhesins, Bacterial; D000818:Animals; D016950:Bordetella bronchiseptica / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D009876:Operon; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017434:Protein Structure, Tertiary; D013552:Swine
chemical_list: D018528:ATP-Binding Cassette Transporters; D037361:Adenylate Cyclase Toxin; D018829:Adhesins, Bacterial