title: Endoplasmic reticulum stress-mediated aldosterone-induced apoptosis in vascular endothelial cells.
abstract: The aim of this study was to examine the effects of endoplasmic reticulum (ER) stress on aldosterone (Aldo)-induced apoptosis of endothelial cells. Glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP, a hallmark of ER-associated apoptosis) were used to evaluate ER stress. Western blotting and real-time PCR were used to analyze indicators of ER molecule. Apoptosis was detected by annexin V/propidium iodide staining and flow cytometry. Human umbilical vein endothelial cells (HUVECs) were stimulated with different concentrations of Aldo for different durations. Aldo promoted apoptosis of HUVECs and induced ER stress, as evidenced by increased expression of GRP78 and CHOP. siRNA knockdown of CHOP attenuated Aldo-mediated apoptosis. These results indicate that ER stress may be involved in Aldo-induced apoptosis of HUVECs.
authors.affiliation: Department of Internal Medicine & Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China. lujp1024@163.com.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Lu
authors.affiliation: Department of Internal Medicine & Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Internal Medicine & Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Ya-Lei
authors.initials: YL
authors.lastname: Jin
authors.affiliation: Department of Internal Medicine & Geriatrics, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China.
authors.forename: Mei-Xiang
authors.initials: MX
authors.lastname: Chen
mesh_terms: D000450:Aldosterone / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D051742:Transcription Factor CHOP / Q000096:biosynthesis
chemical_list: C497249:DDIT3 protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D051742:Transcription Factor CHOP; D000450:Aldosterone