title: Presentation of noise during acute restraint stress attenuates expression of immediate early genes and arginine vasopressin in the hypothalamic paraventricular nucleus but not corticosterone secretion in rats.
abstract: The present study investigated the effect of acoustic stimulation on the activation of the hypothalamic-pituitary-adrenal (HPA) axis in rats submitted to acute restraint stress, through semi-quantitative histochemical analysis of expression of immediate early gene products (c-Fos, JunB and phosphorylated c-Jun) and arginine vasopressin (AVP) hnRNA in the paraventricular nucleus (PVN). Simultaneous presentation of white or pink noise with restraint resulted in a significant attenuation of stress-induced c-Fos and JunB expression in the dorsal body of dorsal medial parvicellular subdivision (mpdd) of the PVN, as compared with restraint without noise. However, this presentation did not change phosphorylation of c-Jun and the plasma corticosterone level. Moreover, white noise presentation during restraint led to a reduction in the number of c-Fos- or JunB-expressing corticotropin-releasing hormone (CRH) neurons and the number of neurons expressing AVP hnRNA in the mpdd. Dual-histochemical labeling revealed co-expression of c-Fos and JunB, as well as JunB and AVP hnRNA in mpdd neurons. These data suggest that acoustic stimuli have an attenuation effect on the restraint-induced activation of neuroendocrine CRH neurons, resulting in the reduction in AVP production as an adaptation of HPA axis to repeated stress.
authors.affiliation: Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Sugimoto
authors.affiliation: Division of Biomedical Information Analysis, Iwate Tohoku Medical Megabank Organization, Iwate Medical University, Shiwa-gun, Iwate, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohmomo
authors.affiliation: Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; Laboratory of Neuroendocrinology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Shutoh
authors.affiliation: Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; Laboratory of Neuroendocrinology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Nogami
authors.affiliation: Laboratory of Neuroendocrinology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; Laboratory of Neuroendocrinology, Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan. Electronic address: shisano@md.tsukuba.ac.jp.
authors.forename: Setsuji
authors.initials: S
authors.lastname: Hisano
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D003345:Corticosterone / Q000097:blood; D017781:Genes, Immediate-Early / Q000502:physiology*; D008297:Male; D009474:Neurons / Q000378:metabolism; D009622:Noise; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D001127:Arginine Vasopressin; D003345:Corticosterone
keywords: Acoustic stimulus; Corticotropin-releasing hormone; JunB; White noise; c-Fos