title: Pyrosequencing analysis of free-living and attached bacterial communities in Meiliang Bay, Lake Taihu, a large eutrophic shallow lake in China.
abstract: To elucidate the relationship between particle-attached (PA, ≥ 5.0 μm) and free-living (FL, 0.2-5.0 μm) bacterial communities, samplings were collected seasonally from November 2011 to August 2012 in Meiliang Bay, Lake Taihu, China. We used 454 pyrosequencing of 16S rRNA genes to study bacterial diversity and structure of PA and FL communities. The analysis rendered 37,985 highly qualified reads, subsequently assigned to 1755 operational taxonomic units (97% similarity) for the 8 samples. Although 27 high-level taxonomic groups were obtained, the 3 dominant phyla (Proteobacteria, Actinobacteria, and Bacteroidetes) comprised about 75.9% and 82.4% of the PA and FL fractions, respectively. Overall, we found no significant differences between community types, as indicated by ANOSIM R statistics (R = 0.063, P > 0.05) and the Parsimony test (P = 0.222). Dynamics of bacterial communities were correlated with changes in concentrations of total suspended solids (TSS) and total phosphorus (TP). In summer, a significant taxonomic overlap in the 2 size fractions was observed when Cyanobacteria, a major contributor of TSS and TP, dominated in the water, highlighting the potential rapid exchange between PA and FL bacterial populations in large shallow eutrophic lakes.
authors.affiliation: a State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, People's Republic of China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Tang
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Keqiang
authors.initials: K
authors.lastname: Shao
authors.forename: Boweng
authors.initials: B
authors.lastname: Wang
authors.forename: Xianlei
authors.initials: X
authors.lastname: Cai
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jianying
authors.initials: J
authors.lastname: Chao
authors.forename: Guang
authors.initials: G
authors.lastname: Gao
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D061006:Bays / Q000032:analysis / Q000382:microbiology*; D044822:Biodiversity; D002681:China; D005068:Eutrophication; D060106:Lakes / Q000032:analysis / Q000382:microbiology*; D008969:Molecular Sequence Data; D010758:Phosphorus / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012621:Seasons; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S; D010758:Phosphorus
keywords: bactéries attachées à des particules; bactéries vivant à l’état libre; free-living bacteria; lac eutrophe peu profond; particle-attached bacteria; pyrosequencing; pyroséquençage; shallow eutrophic lake