title: Saikosaponin D isolated from Bupleurum falcatum inhibits selectin-mediated cell adhesion.
abstract: Three saikosaponins were isolated from the MeOH extract of the roots of Bupleurum falcatum L.: saikosaponins B3 (1); B4 (2); and D (3). Of the three, compound 3 inhibited the interaction of selectins (E, L, and P) and THP-1 cells with IC50 values of 1.8, 3.0 and 4.3 µM, respectively. Also, the aglycone structure 4 of compound 3 showed moderate inhibitory activity on L-selectin-mediated cell adhesion. From these results, we suspect that compound 3 isolated from Bupleurum falcatum roots would be a good candidate for therapeutic strategies to treat inflammation.
authors.affiliation: Enviromental Agriculture Research Division, Gyeonggido Agricultural Reaearch and Extension Services(GARES), Hwaseong, Gyeonggi-do 445-784, Korea. jplant119@gmail.com.
authors.forename: Myoung-Jun
authors.initials: MJ
authors.lastname: Jang
authors.affiliation: Bioindustial Process Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si, Jeollabuk-do 580-185, Korea. yesolkim@kribb.re.kr.
authors.forename: Ye Sol
authors.initials: YS
authors.lastname: Kim
authors.affiliation: LINC project group, University Industry Cooperation, Daejeon University, Daejeon 300-716, Korea. eunjs75@dju.kr.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Bae
authors.affiliation: Department of Plant Resources, Kongju National University, Yesan 340-702, Korea. ots1022@naver.com.
authors.forename: Tae-Seok
authors.initials: TS
authors.lastname: Oh
authors.affiliation: Department of Beauty Science, Kwangju Women's University, Gwangju 506-713, Korea. rerived@kwu.ac.kr.
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: Department of Beauty Science, Kwangju Women's University, Gwangju 506-713, Korea. jh4471@kwu.ac.kr.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Bioindustial Process Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si, Jeollabuk-do 580-185, Korea. ohhm@kribb.re.kr.
authors.forename: Hyun-Mee
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Department of Chemistry, Mokwon University, Daejeon 302-729, Korea. lswdoc@mokwon.ac.kr.
authors.forename: Seung Woong
authors.initials: SW
authors.lastname: Lee
mesh_terms: D029972:Bupleurum / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D016130:Immunophenotyping; D015394:Molecular Structure; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D019087:Selectins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha
chemical_list: D015815:Cell Adhesion Molecules; D010936:Plant Extracts; D012503:Saponins; D019087:Selectins; D014409:Tumor Necrosis Factor-alpha; D009828:Oleanolic Acid; C025759:saikosaponin D