title: Portal vein clamping alone confers protection against hepatic ischemia-reperfusion injury via preserving hepatocyte function in cirrhotic rats.
abstract: BACKGROUND
  Chronic liver diseases always increase the risk of liver failure after hepatectomy. We aimed to explore the protective effect of portal vein clamping without hepatic artery blood control (PVC) on a cirrhotic rat liver that underwent ischemia and reperfusion.
  
  
  METHODS
  Carbon tetrachloride-induced cirrhotic rats were randomly assigned to four groups as follows: cirrhotic control, PVC, portal triad clamping (PTC), and intermittent portal triad clamping (IC). After 45 min of portal vascular clamping, hepatic injury and liver function were investigated by assessing the 7-d survival rate, liver blood loss, serum alanine aminotransferase, liver tissue malondialdehyde, liver tissue adenosine triphosphate, indocyanine green retention rate, and morphology changes of the rat liver.
  
  
  RESULTS
  The 7-d survival rates in the PVC and IC groups were much higher than in the PTC group. The PVC group had more liver blood loss during the hepatectomy than the PTC group, but had much less than the cirrhotic control group (P < 0.01). In addition, there were no differences between the IC group and PVC group. The PVC rats had a significantly higher adenosine triphosphate level in the liver tissue and a markedly lower indocyanine green retention rate than the PTC and IC rats (P < 0.05). At 1, 6, and 24 h after reperfusion, the alanine aminotransferase and malondialdehyde levels in the PTC group were much higher than those in the PVC and IC groups (P < 0.05). Based on the histopathologic analysis, hepatic injury in the PVC and IC groups were similar but less prominent than in the PTC group.
  
  
  CONCLUSIONS
  Although both PVC and IC can confer protection against hepatic ischemic-reperfusion injury in cirrhotic rats, the PVC method is more efficient in preserving the energy and function of hepatocytes than the IC method, suggesting better prognosis after hepatectomy.
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Jian Jun
authors.initials: JJ
authors.lastname: Hu
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Chong Hui
authors.initials: CH
authors.lastname: Li
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Hong Dong
authors.initials: HD
authors.lastname: Wang
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Wen Li
authors.initials: WL
authors.lastname: Xu
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China.
authors.forename: Ai Qun
authors.initials: AQ
authors.lastname: Zhang
authors.affiliation: Department & Institute of Hepatobiliary Surgery, Chinese PLA General Hospital, Beijing, China. Electronic address: Dongjh301@163.com.
authors.forename: Jia Hong
authors.initials: JH
authors.lastname: Dong
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D022781:Hepatocytes / Q000502:physiology*; D008099:Liver / Q000098:blood supply*; D008106:Liver Cirrhosis, Experimental / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D011169:Portal Vein / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*
chemical_list: D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D000410:Alanine Transaminase
keywords: CCl4; Cirrhosis; Hepatic artery; Ischemia–reperfusion; Liver; Portal vein