title: The effects of initial substrate concentration, C/N ratio, and temperature on solid-state anaerobic digestion from composting rice straw.
abstract: This study investigated the possibilities of improving the biogasification from solid-state anaerobic digestion (SS-AD) of composting rice straw (RS) based on the optimized digestion temperature, initial substrate concentration (ISC) and C/N ratio. RS compounds, such as lignin, cellulose, and hemicellulose, were significantly degraded after composting. A significant interactive effect of temperature, ISC and C/N ratio was found on the biogasification of SS-AD of composting RS, and a maximum biogas production was achieved at 35.6°C, with a 20% ISC and a C/N ratio of 29.6:1. The verification experiment confirmed the optimization results. High-throughput sequencing analysis indicated that microbial communities in the SS-AD mainly consist of Methanobacteria, Bacteroidia, Clostridia, Betaproteobacteria, and Gammaproteobacteria. A dominant Methanobacteria was shifted from Methanobacterium to Methanoculleus during the SS-AD process. This study provides novel information about the interdependent effects and microbial behavior of AD.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Song
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China.
authors.forename: Yuexiang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041, China. Electronic address: lxf3636@163.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Nanjing Normal University, Nanjing 210023, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zheng
mesh_terms: D000693:Anaerobiosis; D000704:Analysis of Variance; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D056804:Biofuels / Q000032:analysis; D002244:Carbon / Q000032:analysis*; D014644:Genetic Variation; D059014:High-Throughput Nucleotide Sequencing; D008697:Methane / Q000032:analysis; D008962:Models, Theoretical; D009584:Nitrogen / Q000032:analysis*; D012275:Oryza / Q000737:chemistry*; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012037:Refuse Disposal / Q000379:methods*; D012987:Soil; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014866:Waste Products
chemical_list: D056804:Biofuels; D012336:RNA, Ribosomal, 16S; D012987:Soil; D014866:Waste Products; D002244:Carbon; D009584:Nitrogen; D008697:Methane
keywords: Anaerobic digestion; Composting; Optimization; Sequencing; Solid-state