title: Comparison of mesenchymal stem cell markers in multiple human adult stem cells.
abstract: OBJECTIVE
  Mesenchymal stem cells (MSCs) are adult stem cells which identified by adherence to plastic, expression of cell surface markers including CD44, CD90, CD105, CD106, CD166, and Stro-1, lack of the expression of hematopoietic markers, no immunogenic effect and replacement of damaged tissues. These properties led to development of progressive methods to isolation and characterization of MSCs from various sources for therapeutic applications in regenerative medicine.
  
  
  METHODS
  We isolated MSC-like cells from testis biopsies, ovary, hair follicle and umbilical cord Wharton's jelly and investigated the expression of specific cell surface antigens using flow cytometry in order to verify stemness properties of these cells.
  
  
  RESULTS
  All four cell types adhered to plastic culture flask a few days after primary culture. All our cells positively expressed common MSC- specific cell surface markers. Moreover, our results revealed the expression of CD19and CD45 antigens in these cells.
  
  
  CONCLUSIONS
  According to our results, high expression of CD44 in spermatogonial stem cells (SSCs), hair follicle stem cells (HFSCs),granulosa cells (GCs)and Wharton's jelly- MSCs (WJ-MSCs)may help them to maintain stemness properties. Furthermore, we suggest that CD105+SSCs, HFSCs and WJ-MSCs revealed the osteogenic potential of these cells. Moreover, high expression of CD90 in SSCs and HFSCs may associate to higher growth and differentiation potential of these cells. Further, the presence of CD19 on SSCs and GCs may help them to efficiency in response to trans-membrane signals. Thus, these four types of MSCs may be useful in clinical applications and cell therapy.
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran ; Stem Cell Research Lab, Azarbaijan ART Centre, ACECR East Azarbaijan Branch, Tabriz, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Maleki
authors.affiliation: Stem Cell Research Lab, Azarbaijan ART Centre, ACECR East Azarbaijan Branch, Tabriz, Iran.
authors.forename: Farideh
authors.initials: F
authors.lastname: Ghanbarvand
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Reza Behvarz
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Ejtemaei
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Ghadirkhomi
keywords: Cell Surface Markers; Flow Cytometry; Mesenchymal Stem Cells