title: Physicochemical comparison of chitin and chitosan obtained from larvae and adult Colorado potato beetle (Leptinotarsa decemlineata).
abstract: Chitins and chitosans obtained from larva and adult Colorado potato beetles (Leptinotarsa decemlineata) were physico-chemically characterized and differences between adults and larvae were identified. The dry weight chitin contents of the adult Colorado potato beetles and larvae were determined as 20% and 7%, respectively. The chitin produced chitosan yields of 72% from the adult Colorado potato beetles and 67% from the larvae. FTIR analysis showed that the isolated chitins were in the alpha form. Crystalline index values, determined by XRD, were 72% for larvae and 76% for adults. The degradation temperatures of the isolated chitin structures were measured by TGA, and this showed that the chitin from adult Colorado potato beetles had a more stable structure than that from the larvae. The surface morphologies of the isolated chitin and chitosan structures were analysed with SEM and it was revealed that these structures consisted of nanofibres. According to elemental analysis, the purity of chitin and chitosan from adults was greater than that from the larvae. The results of molecular analysis showed that the chitosans from adults (2.722 kDa) and larvae (2.676 kDa) of the Colorado potato beetle have low molecular weights. Antimicrobial and antioxidant activities of both adult and larval chitosans were determined. The adult potato beetle is more appropriate than the larvae as an alternative chitin source because of the fact that its dry weight chitin content, chitosan yield and purity of chitin are higher than those from the larvae, and its antimicrobial and antioxidant activities are also higher than those from the larvae.
authors.affiliation: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey. Electronic address: muratkaya3806@yahoo.com.
authors.forename: Murat
authors.initials: M
authors.lastname: Kaya
authors.affiliation: Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey; Department of Chemistry, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey. Electronic address: talatbaran66@hotmail.com.
authors.forename: Talat
authors.initials: T
authors.lastname: Baran
authors.affiliation: Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey; Trakya University, Keşan Vocational College, Fisheries Programme, 22800 Keşan, Edirne, Turkey. Electronic address: serdogantrakya@gmail.com.
authors.forename: Sevil
authors.initials: S
authors.lastname: Erdoğan
authors.affiliation: Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey; Department of Chemistry, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey. Electronic address: amentes@aksaray.edu.tr.
authors.forename: Ayfer
authors.initials: A
authors.lastname: Menteş
authors.affiliation: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey. Electronic address: meltemozusaglam@gmail.com.
authors.forename: Meltem Aşan
authors.initials: MA
authors.lastname: Özüsağlam
authors.affiliation: Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey; Aksaray University, Science and Technology Application and Research Center, 68100 Aksaray, Turkey. Electronic address: yavuzselimcakmak@gmail.com.
authors.forename: Yavuz Selim
authors.initials: YS
authors.lastname: Çakmak
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism; D001407:Bacillus / Q000187:drug effects; D002175:Candida / Q000187:drug effects; D002686:Chitin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001517:Coleoptera / Q000254:growth & development*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D007814:Larva / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D002686:Chitin; D048271:Chitosan
keywords: Biomaterial; FTIR; Nanofibre; Porosity; TGA; XRD