title: Microfluidic model of angiogenic sprouting.
abstract: Microfluidic systems have emerged as an important technology for modeling cellular microenvironments in vitro. These systems enable unprecedented levels of control of chemical gradients, fluid flow, and localized 3-D extracellular matrices (ECM), all of which can be integrated to provide a physiologically relevant context for studying complex cellular processes such as angiogenesis. Here, we describe the design and use of microfluidic systems for reproducing the dynamic events of vascular morphogenesis.
authors.affiliation: Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, MA, 02129, USA.
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Song
authors.forename: Despina
authors.initials: D
authors.lastname: Bazou
authors.forename: Lance L
authors.initials: LL
authors.lastname: Munn
mesh_terms: D018929:Cell Culture Techniques; D002470:Cell Survival; D004129:Dimethylpolysiloxanes; D020724:Elastomers; D005455:Fluorescent Antibody Technique; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D057054:Molecular Imaging; D018919:Neovascularization, Physiologic
chemical_list: D004129:Dimethylpolysiloxanes; D020724:Elastomers; C013830:baysilon