title: Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells.
abstract: BACKGROUND
  In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells.
  
  
  METHODS
  Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.
  
  
  RESULTS
  Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion.
  
  
  CONCLUSIONS
  These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Yong Pill
authors.initials: YP
authors.lastname: Yoon
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jiho
authors.initials: J
authors.lastname: Ryu
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Su Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Hyun Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy, Yeungnam University, Daegu, Korea.
authors.forename: Seungho
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Pharmacy, College of Pharmacy, Seoul National University, Seoul, Korea.
authors.forename: Sang Kook
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Pulmonology Section, Department of Internal Medicine, Chungnam National University Hospital, Daejeon, Korea.
authors.forename: Ju-Ock
authors.initials: JO
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Seok
authors.affiliation: Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea.
authors.forename: Choong Jae
authors.initials: CJ
authors.lastname: Lee
keywords: MUC5AC Protein, Human; Methyl Linoleate; Mucins