title: Bacterial spectrum colonizing chronic leg ulcers: a 10-year comparison from a German wound care center.
abstract: OBJECTIVE
  Chronic wounds are colonized by many different bacteria.
  
  
  METHODS
  We analyzed microbiological swabs from chronic leg ulcers from 2012/2013 and matched the results with those from 2002/2003 and 2007/2008 from the same institution.
  
  
  RESULTS
  Results from 100 patients from our specialized wound care center were evaluated retrospectively. The etiologies were broadly variable with chronic venous insufficiency being the most common. As the most common bacterium Staphylococcus aureus was found in 53% of patients; 9% of patients were positive for methicillin-resistant S. aureus (MRSA). Pseudomonas aeruginosa was detected in 25% of patients. Different fecal bacteria and Enterobacteriaceae were found in 49% of the patients; 11% of the swabs were sterile. The detection rate for S. aureus has regressed by 17.1% over the past decade and in the case of MRSA by 12.5%. In contrast, colonization with gram-negative bacteria species significantly increased by 11.7%. This tendency was also seen in 2007/2008, but with a higher count of P. aeruginosa.
  
  
  CONCLUSIONS
  Although S. aureus is still the most frequently detected bacterium in our wound care center, there has been a shift in the bacterial spectrum from gram-positive towards gram-negative bacteria over the last decade.
authors.affiliation: Department of Dermatology, Venereology and Allergology, University Hospital Essen, Germany.
authors.forename: Finja
authors.initials: F
authors.lastname: Jockenhöfer
authors.forename: Valérie
authors.initials: V
authors.lastname: Chapot
authors.forename: Maren
authors.initials: M
authors.lastname: Stoffels-Weindorf
authors.forename: Andreas
authors.initials: A
authors.lastname: Körber
authors.forename: Joachim
authors.initials: J
authors.lastname: Klode
authors.forename: Jan
authors.initials: J
authors.lastname: Buer
authors.forename: Bernhard
authors.initials: B
authors.lastname: Küpper
authors.forename: Alexander
authors.initials: A
authors.lastname: Roesch
authors.forename: Joachim
authors.initials: J
authors.lastname: Dissemond
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D002908:Chronic Disease; D003880:Dermatology / Q000706:statistics & numerical data; D005260:Female; D005858:Germany; D006801:Humans; D007871:Leg Ulcer / Q000453:epidemiology* / Q000382:microbiology*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D014193:Trauma Centers / Q000706:statistics & numerical data