title: New type of BACE1 siRNA delivery to cells.
abstract: BACKGROUND
  Small interfering RNA (siRNA) gene therapy is a new molecular approach in the search for an efficient therapy for Alzheimer disease (AD), based on the principle of RNA interference. Reducing BACE activity can have great therapeutic potential for the treatment of AD. In this study, receptor-mediated delivery was used to deliver opioid peptide-conjugated BACE 1 to INR-32 human neuroblastoma cells.
  
  
  METHODS
  An INR-32 human neuroblastoma cell line was stably transfected to express the APP cDNA coding fragment containing the predicted sites for cleavage by α, β, or γ-secretase. This was then treated with BACE 1 siRNA to silence BACE gene expression. BACE gene transcription and translation was determined using BACE-1 siRNA cross-linked with opioid peptide, together with RT-PCR, Western blot analysis, and ELISA.
  
  
  RESULTS
  Receptor-mediated delivery was used to introduce BACE1 siRNA to the APP - INR 32 human neuroblastoma cells. Decreased BACE mRNA and protein expression were observed after the cells were transfected with BACE1 siRNA.
  
  
  CONCLUSIONS
  Delivery of BACE1 siRNA appears to specifically reduce the cleavage of APP by inhibiting BACE1 activity.
authors.affiliation: Department of Infectious and Liver Diseases, Medical University of Łódź, Łódź, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jabłkowski
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szemraj
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Oszajca
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Janiszewska
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Bartkowiak
authors.affiliation: Department of Medical Biochemistry, Medical University of Łódź, Łódź, Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Szemraj
mesh_terms: D053829:Amyloid Precursor Protein Secretases / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics / Q000378:metabolism; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000378:metabolism*; D001681:Biological Assay; D015153:Blotting, Western; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D011957:Receptors, Opioid / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C545487:APP protein, human; D016564:Amyloid beta-Protein Precursor; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D011957:Receptors, Opioid; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human