title: Virtual karyotyping reveals greater chromosomal stability in neural cells derived by transdifferentiation than those from stem cells.
abstract: Neural cells can be derived either from pluripotent or adult stem cells via differentiation or by transdifferentiation from other cell types with the aid of tissue regulators. We compared the chromosomal stability of over 500 neural cell samples from human and mouse with virtual karyotyping (e-karyotyping). We detected notable genomic instability in cells derived from pluripotent or adult stem cells, but surprisingly, transdifferentiated cells seemed more chromosomally stable, except if they were reprogrammed using pluripotency factors.
authors.affiliation: Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Weissbein
authors.affiliation: Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
authors.forename: Uri
authors.initials: U
authors.lastname: Ben-David
authors.affiliation: Azrieli Center for Stem Cells and Genetic Research, Department of Genetics, Silberman Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel. Electronic address: nissimb@cc.huji.ac.il.
authors.forename: Nissim
authors.initials: N
authors.lastname: Benvenisty
mesh_terms: D053687:Adult Stem Cells / Q000502:physiology*; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D054338:Cell Transdifferentiation; D065150:Cellular Reprogramming; D043171:Chromosomal Instability; D002869:Chromosome Aberrations / Q000706:statistics & numerical data*; D019295:Computational Biology / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007621:Karyotyping; D051379:Mice; D009474:Neurons / Q000502:physiology*; D059467:Transcriptome