title: An LC-MS/MS method for the simultaneous determination of lycorine and galanthamine in rat plasma and its application to pharmacokinetic study of Lycoris radiata extract in rats.
abstract: A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometry was developed for the simultaneous determination of lycorine and galanthamine, two major constituents in Lycoris radiata extract, in rat plasma. Liquid-liquid extraction with ethyl ether was carried out using diphenhydramine as the internal standard. The two bioactive alkaloids were separated on a Zorbax SB-C18 reserved-phase column (150 mm × 4.6 mm, i.d., 5 μm) by gradient elution using a mobile phase consisting of methanol with 0.1% formic acid (A) and water with 0.1% formic acid (B) at a flow rate of 0.6 mL/min. All analytes showed good linearity over a wide concentration range (r (2)>0.99) and the lower limit of quantification was 3.00 ng/mL for each analyte. The average extraction recovery of the analytes from rat plasma was more than 82.15%, and the intra-day and inter-day accuracy and precision of the assay were less than 12.6%. The validated method was successfully applied to monitoring the concentrations and pharmacokinetic studies of two Amaryllidaceous alkaloids in rat plasma after an oral administration of Lycoris radiata extract.
authors.affiliation: Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China.
authors.forename: Yue-Bin
authors.initials: YB
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Pharmacy, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, 350025, China. yxkliuying@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000493:pharmacokinetics*; D000818:Animals; D002853:Chromatography, Liquid; D005702:Galantamine / Q000493:pharmacokinetics*; D031426:Lycoris / Q000737:chemistry*; D008297:Male; D010277:Parasympathomimetics / Q000493:pharmacokinetics*; D010617:Phenanthridines / Q000493:pharmacokinetics*; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D047151:Amaryllidaceae Alkaloids; D010277:Parasympathomimetics; D010617:Phenanthridines; D010936:Plant Extracts; D005702:Galantamine; C015330:lycorine