title: Formulations for modulation of protein release from large-size PLGA microparticles for tissue engineering.
abstract: In this study we present an approach to pre-program lysozyme release from large size (100-300 μm) poly(DL-lactic acid-co-glycolic acid) (PLGA) microparticles. This approach involved blending in-house synthesized triblock copolymers with a PLGA 85:15. In this work it is demonstrated that the lysozyme release rate and the total release are related to the mass of triblock copolymer present in polymer formulation. Two triblock copolymers (PLGA-PEG1500-PLGA and PLGA-PEG1000-PLGA) were synthesized and used in this study. In a like-for-like comparison, these two triblock copolymers appeared to have similar effects on the release of lysozyme. It was shown that blending resulted in the increase of the total lysozyme release and shortened the release period (70% release within 30 days). These results demonstrated that blending PLGA-PEG-PLGA triblock copolymer with PLGA 85:15 can be used as a method to pre-program protein release from microparticles. These microparticles with modulated protein release properties may be used to create microparticle-based tissue engineering constructs with pre-programmed release properties.
authors.affiliation: Division of Drug Delivery and Tissue Engineering, Wolfson Centre for Stem Cell, Tissue Engineering and Modelling, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK; TherapeuTech Ltd., University of Nottingham Institute for Enterprise and Innovation, Sir Colin Campbell Building, Jubilee Campus, Nottingham NG7 2TU, UK. Electronic address: Roozbeh.qodratnama@gmail.com.
authors.forename: Roozbeh
authors.initials: R
authors.lastname: Qodratnama
authors.affiliation: Neotherix Ltd. Research Centre, York Science Park, York YO10 5DF, UK.
authors.forename: Lorenzo Pio
authors.initials: LP
authors.lastname: Serino
authors.affiliation: Division of Drug Delivery and Tissue Engineering, Wolfson Centre for Stem Cell, Tissue Engineering and Modelling, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: Helen C
authors.initials: HC
authors.lastname: Cox
authors.affiliation: Division of Drug Delivery and Tissue Engineering, Wolfson Centre for Stem Cell, Tissue Engineering and Modelling, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: Omar
authors.initials: O
authors.lastname: Qutachi
authors.affiliation: Division of Drug Delivery and Tissue Engineering, Wolfson Centre for Stem Cell, Tissue Engineering and Modelling, School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, UK.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: White
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D004337:Drug Carriers; D011108:Polymers; D011506:Proteins; C528571:poly(lactic-co-glycolic acid)-polyethylene glycol-poly(lactic-co-glycolic acid); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011098:Polyglactin 910; D011092:Polyethylene Glycols
keywords: Controlled release; Glass transition temperature; Lysozyme; Microparticle; PLGA–PEG–PLGA; Triblock copolymer