title: Towards Elucidating the Effects of Purified MWCNTs on Human Lung Epithelial cells.
abstract: Toxicity of engineered nanomaterials is associated with their inherent properties, both physical and chemical. Recent studies have shown that exposure to multi-walled carbon nanotubes (MWCNTs) promotes tumors and tumor-associated pathologies and lead to carcinogenesis in modelin vivosystems. Here in we examined the potential of purified MWCNTs used at occupationally relevant exposure doses for particles not otherwise regulated to affect human lung epithelial cells. The uptake of the purified MWCNTs was evaluated using fluorescence activated cell sorting (FACS), while the effects on cell fate were assessed using 2- (4-iodophenyl) - 3- (4-nitrophenyl) - 5-(2, 4-disulfophenyl) -2H-tetrazolium salt colorimetric assay, cell cycle and nanoindentation. Our results showed that exposure to MWCNTs reduced cell metabolic activity and induced cell cycle arrest. Our analysis further emphasized that MWCNTs-induced cellular fate results from multiple types of interactions that could be analyzed by means of intracellular biomechanical changes and are pivotal in understanding the underlying MWCNTs-induced cell transformation.
authors.affiliation: Department of Chemical Engineering, West Virginia University, Morgantown WV, 26506, USA.
authors.forename: Chenbo
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Chemical Engineering, West Virginia University, Morgantown WV, 26506, USA.
authors.forename: Reem
authors.initials: R
authors.lastname: EIdawud
authors.affiliation: National Institute for Occupational Safety and Health, Morgantown WV, 26505, USA.
authors.forename: Linda M
authors.initials: LM
authors.lastname: Sargent
authors.affiliation: National Institute for Occupational Safety and Health, Morgantown WV, 26505, USA.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Kashon
authors.affiliation: National Institute for Occupational Safety and Health, Morgantown WV, 26505, USA.
authors.forename: David
authors.initials: D
authors.lastname: Lowry
authors.affiliation: Department of Basic Pharmaceutical Sciences, West Virginia University, Morgantown WV, 26506, USA.
authors.forename: Yon
authors.initials: Y
authors.lastname: Rojanasakul
authors.affiliation: Department of Chemical Engineering, West Virginia University, Morgantown WV, 26506, USA.
authors.forename: Cerasela Zoica
authors.initials: CZ
authors.lastname: Dinu
keywords: Carbon nanotubes; biomechanical changes; cell cycle arrest; cyto-genotoxic effects