title: Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids.
abstract: Oxidation of polyunsaturated fatty acids (PUFA) through enzymatic or non-enzymatic free radical-mediated reactions can yield an array of lipid metabolites including eicosanoids, octadecanoids, docosanoids and related species. In mammals, these oxygenated PUFA mediators play prominent roles in the physiological and pathological regulation of many key biological processes in the cardiovascular, renal, reproductive and other systems including their pivotal contribution to inflammation. Mass spectrometry-based technology platforms have revolutionized our ability to analyze the complex mixture of lipid mediators found in biological samples, with increased numbers of metabolites that can be simultaneously quantified from a single sample in few analytical steps. The recent development of high-sensitivity and high-throughput analytical tools for lipid mediators affords a broader view of these oxygenated PUFA species, and facilitates research into their role in health and disease. In this review, we illustrate current analytical approaches for a high-throughput lipidomic analysis of eicosanoids and related mediators in biological samples. This article is part of a Special Issue entitled "Oxygenated metabolism of PUFA: analysis and biological relevance."
authors.affiliation: Waters Corporation, Milford, MA, USA; Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, DC, USA. Electronic address: giuseppe_astarita@waters.com.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Astarita
authors.affiliation: Manchester Pharmacy School, Faculty of Medical and Human Sciences, The University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Kendall
authors.affiliation: Department of Chemistry/Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0601, USA; Department of Pharmacology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0601, USA.
authors.forename: Edward A
authors.initials: EA
authors.lastname: Dennis
authors.affiliation: Manchester Pharmacy School, Faculty of Medical and Human Sciences, The University of Manchester, Stopford Building, Oxford Road, Manchester M13 9PT, UK. Electronic address: anna.nicolaou@manchester.ac.uk.
authors.forename: Anna
authors.initials: A
authors.lastname: Nicolaou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D057166:High-Throughput Screening Assays; D006801:Humans; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D015398:Signal Transduction; D049490:Systems Biology
chemical_list: D015415:Biomarkers; D005231:Fatty Acids, Unsaturated
keywords: Eicosanoid; Lipid mediator; Lipidomics; Mass spectrometry; PUFA, polyunsaturated fatty acid; Prostaglandin