title: Next-generation sequencing of 34 genes in sudden unexplained death victims in forensics and in patients with channelopathic cardiac diseases.
abstract: Sudden cardiac death (SCD) is responsible for a large proportion of sudden deaths in young individuals. In forensic medicine, many cases remain unexplained after routine postmortem autopsy and conventional investigations. These cases are called sudden unexplained deaths (SUD). Genetic testing has been suggested useful in forensic medicine, although in general with a significantly lower success rate compared to the clinical setting. The purpose of the study was to estimate the frequency of pathogenic variants in the genes most frequently associated with SCD in SUD cases and compare the frequency to that in patients with inherited cardiac channelopathies. Fifteen forensic SUD cases and 29 patients with channelopathies were investigated. DNA from 34 of the genes most frequently associated with SCD were captured using NimbleGen SeqCap EZ library build and were sequenced with next-generation sequencing (NGS) on an Illumina MiSeq. Likely pathogenic variants were identified in three out of 15 (20%) forensic SUD cases compared to 12 out of 29 (41%) patients with channelopathies. The difference was not statistically significant (p = 0.1). Additionally, two larger deletions of entire exons were identified in two of the patients (7%). The frequency of likely pathogenic variants was >2-fold higher in the clinical setting as compared to SUD cases. However, the demonstration of likely pathogenic variants in three out of 15 forensic SUD cases indicates that NGS investigations will contribute to the clinical investigations. Hence, this has the potential to increase the diagnostic rate significantly in the forensic as well as in the clinical setting.
authors.affiliation: Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 11 Frederik V's Vej, 2100, Copenhagen, Denmark, christin.hertz@sund.ku.dk.
authors.forename: C L
authors.initials: CL
authors.lastname: Hertz
authors.forename: S L
authors.initials: SL
authors.lastname: Christiansen
authors.forename: L
authors.initials: L
authors.lastname: Ferrero-Miliani
authors.forename: S L
authors.initials: SL
authors.lastname: Fordyce
authors.forename: M
authors.initials: M
authors.lastname: Dahl
authors.forename: A G
authors.initials: AG
authors.lastname: Holst
authors.forename: G L
authors.initials: GL
authors.lastname: Ottesen
authors.forename: R
authors.initials: R
authors.lastname: Frank-Hansen
authors.forename: H
authors.initials: H
authors.lastname: Bundgaard
authors.forename: N
authors.initials: N
authors.lastname: Morling
mesh_terms: D000293:Adolescent; D000328:Adult; D053447:Channelopathies / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D016757:Death, Sudden, Cardiac / Q000209:etiology*; D005260:Female; D053803:Forensic Genetics; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D054643:INDEL Mutation; D007223:Infant; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D055815:Young Adult