title: Lead induced changes in phosphorylation of PSII proteins in low light grown pea plants.
abstract: Light-intensity and redox-state induced thylakoid proteins phosphorylation involved in structural changes and in regulation of protein turnover. The presence of heavy metal ions triggers a wide range of cellular responses including changes in plant growth and photosynthesis. Plants have evolved a number of mechanisms to protect photosynthetic apparatus. We have characterized the effect of lead on PSII protein phosphorylation in pea (Pisum sativum L.) plants grown in low light conditions. Pb ions affected only slightly photochemical efficiency of PSII and had no effect on organization of thylakoid complexes. Lead activated strongly phosphorylation of PSII core D1 protein and dephosphorylation of this protein did not proceed in far red light. D1 protein was also not degraded in this conditions. However, phosphorylation of LHCII proteins was not affected by lead. These results indicate that Pb(2+) stimulate the phosphorylation of PSII core proteins and by disturbing the disassembly of supercomplexes play a role in PSII repair mechanism. LHCII phosphorylation could control the distribution of energy between the photosystems in low light conditions. This demonstrates that plants may respond to heavy metals by induction different pathways responsible for protein protection under stress conditions.
authors.affiliation: Department of Molecular Plant Physiology, Faculty of Biology, Warsaw University, Miecznikowa 1, 02096, Warsaw, Poland.
authors.forename: Wasilewska
authors.initials: W
authors.lastname: Wioleta
authors.forename: Drożak
authors.initials: D
authors.lastname: Anna
authors.forename: Bacławska
authors.initials: B
authors.lastname: Ilona
authors.forename: Kąkol
authors.initials: K
authors.lastname: Kamila
authors.forename: Romanowska
authors.initials: R
authors.lastname: Elżbieta
mesh_terms: D007854:Lead / Q000633:toxicity*; D008027:Light; D018532:Pisum sativum / Q000187:drug effects* / Q000378:metabolism*; D010766:Phosphorylation; D010788:Photosynthesis / Q000187:drug effects; D045332:Photosystem II Protein Complex / Q000187:drug effects / Q000378:metabolism; D020524:Thylakoids / Q000187:drug effects / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; D007854:Lead