title: Lignans from the stems and leaves of Brandisia hancei and their effects on VEGF-induced vascular permeability and migration of HRECs and DLAV formation in zebrafish.
abstract: In our continuing search for novel antiangiogenic agents, a new lignan glycoside, (7R,8R)-1-(4-O-β-d-glucopyranosyl-3-methoxyphenyl)-2-{2-methoxy-4-[1-(E)-propene-3-ol]-phenoxyl}-propane-1,3-diol (1), along with three known lignans (2-4), were isolated from the 80% EtOH extract of Brandisia hancei stems and leaves. These isolates (1-4) were subjected to an in vitro bioassay to evaluate their effects on vascular endothelial growth factor (VEGF)-induced vascular permeability and migration of human retinal endothelial cells (HRECs). Of the compounds tested, compound 1 resulted in the greatest reduction in VEGF-induced vascular permeability by about 31.5% at 10 μM compared to the VEGF-treated control. In the migration assay, compounds 1 and 2 significantly decreased VEGF-induced HREC migration. Furthermore, zebrafish embryos treated with compounds 1 and 2 showed mild reductions of dorsal longitudinal anastomotic vessel (DLAV) formation.
authors.affiliation: a KM-Based Herbal Drug Development Group, Herbal Medicine Research Division , Korea Institute of Oriental Medicine , Daejeon , Republic of Korea.
authors.forename: Ik-Soo
authors.initials: IS
authors.lastname: Lee
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Seung-Hyun
authors.initials: SH
authors.lastname: Jung
authors.forename: Song Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Joo-Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Hang
authors.initials: H
authors.lastname: Sun
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D001681:Biological Assay; D001808:Blood Vessels / Q000187:drug effects* / Q000196:embryology; D002199:Capillary Permeability / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D004625:Embryo, Nonmammalian / Q000098:blood supply / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D000068378:Lamiales / Q000737:chemistry; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D009024:Morphogenesis / Q000187:drug effects / Q000235:genetics; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015027:Zebrafish
chemical_list: D020533:Angiogenesis Inhibitors; D006027:Glycosides; D017705:Lignans; D010936:Plant Extracts; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Brandisia hancei; Scrophulariaceae; lignans; vascular endothelial growth factor; zebrafish