title: Growth optimisation of microalga mutant at high CO₂ concentration to purify undiluted anaerobic digestion effluent of swine manure.
abstract: Growth rate of the microalga Chlorella PY-ZU1 mutated by nuclear irradiation was optimised for use in the purification of undiluted anaerobic digestion effluent of swine manure (UADESM) with 3745 mg L(-1) chemical oxygen demand (COD) and 1135 mg L(-1) total nitrogen content. The problem of accessible carbon in UADESM was solved by continuous introduction of 15% (v/v) CO2. Adding phosphorus to UADESM and aeration of UADESM before inoculation both markedly reduced the lag phase of microalgal growth. In addition, the biomass yield and average growth rate of Chlorella PY-ZU1 increased significantly to 4.81 g L(-1) and 601.2 mg L(-1) d(-1), respectively, while the removal efficiencies of total phosphorus, COD and ammonia nitrogen increased to 95%, 79% and 73%, respectively. Thus, the findings indicate that Chlorella PY-ZU1 can be used for effective purification of UADESM, while the biomass can be safely used as animal feed supplement.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan.
authors.forename: Yuyou
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D000693:Anaerobiosis / Q000187:drug effects; D000818:Animals; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D002467:Cell Nucleus / Q000528:radiation effects; D002708:Chlorella / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D005720:Gamma Rays; D008372:Manure / Q000032:analysis*; D019216:Metals, Heavy / Q000302:isolation & purification; D058086:Microalgae / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000528:radiation effects; D009154:Mutation / Q000235:genetics*; D013552:Swine; D014865:Waste Disposal, Fluid; D014874:Water Pollutants, Chemical / Q000302:isolation & purification; D018508:Water Purification / Q000379:methods*
chemical_list: D008372:Manure; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D002245:Carbon Dioxide
keywords: Anaerobic digestion effluent; High CO(2) concentration; Microalgae; Nuclear irradiation; Pollutant removal