title: Analytical investigations on the lindane bioremediation capability of the demosponge Hymeniacidon perlevis.
abstract: Lindane is an organochlorine pesticide that has been widely used to treat agricultural pests. It is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. In this context, we investigated the ability of the demosponge Hymeniacidon perlevis to bioremediate lindane polluted seawater during in vitro experimentation. Lindane was extracted by solid-phase micro-extraction (SPME) and determined by gas chromatography-mass spectrometry (GC-MS). Furthermore, we assessed the role exerted in lindane degradation by bacteria isolated from the sponge. Sponges showed low mortality in experimental conditions (lindane concentration 1 μg/L) and were able to remove about 50% of the lindane content from seawater in 48 h. Bacteria isolated from sponges showed a remarkable remediating capacity (up to 97% of lindane removed after 8-days). A lindane metabolite was identified, 1,3,4,5,6-pentachloro-cyclohexene. The results obtained are a prelude to the development of future strategies for the in situ bioremediation of this pollutant.
authors.affiliation: Department of Chemistry, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy; Interdepartmental Research Center S.M.A.R.T., University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: antonellamaria.aresta@uniba.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Aresta
authors.affiliation: Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: carlotta.nonnismarzano@uniba.it.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Nonnis Marzano
authors.affiliation: Department of Chemistry, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: chiaralopane@live.it.
authors.forename: Chiara
authors.initials: C
authors.lastname: Lopane
authors.affiliation: Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: giuseppe.corriero@uniba.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Corriero
authors.affiliation: Department of Biology, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: caterina.longo@uniba.it.
authors.forename: Caterina
authors.initials: C
authors.lastname: Longo
authors.affiliation: Department of Chemistry, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy; Interdepartmental Research Center S.M.A.R.T., University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy. Electronic address: carlo.zambonin@uniba.it.
authors.forename: Carlo
authors.initials: C
authors.lastname: Zambonin
authors.affiliation: DiSTeBa, University of Salento, Via Prov.le Lecce-Monteroni, 73100 Lecce, Italy; IAMC, Section of Taranto-CNR, Via Roma, 3-70400 Taranto, Italy. Electronic address: loredana.stabili@iamc.cnr.it.
authors.forename: Loredana
authors.initials: L
authors.lastname: Stabili
mesh_terms: D000383:Agriculture; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D008401:Gas Chromatography-Mass Spectrometry; D001556:Hexachlorocyclohexane / Q000032:analysis / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis; D010575:Pesticides / Q000032:analysis; D011161:Porifera / Q000378:metabolism* / Q000382:microbiology; D012623:Seawater / Q000737:chemistry*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010575:Pesticides; D001556:Hexachlorocyclohexane
keywords: Bioremediation; Gas chromatography–mass spectrometry; Hymeniacidon perlevis; Lindane; Solid-phase microextraction