title: Nocardioides paucivorans sp. nov. isolated from soil.
abstract: One strain, designated KIS31-44(T), was isolated from a soil sample collected from Dokdo Island, South Korea. The strain is Gram-stain-positive, aerobic, non-spore-forming and non-motile. It grows optimally at 28-30°C, at pH 7.0 and 0% NaCl. 16S rRNA gene sequence analysis showed that strain KIS31-44(T) belonged to the genus Nocardioides and shared the highest sequence similarities with Nocardioides aestuarii JC2056(T) (95.5%) and Nocardioides terrae VA15(T) (95.0%). The major fatty acids of strain KIS31-44(T) were C(17:1) ω6c, C(18:1) ω9c, summed feature 3 (C(16:1) ω6c and/or C(16:1) ω7c), iso-C(16:0), C(18:0) 10-methyl (TBSA), C(16:0) 2-OH, C(17:0) 10-methyl, and iso-C(16:1) H. The major isoprenoid quinone was MK-8 (H₄). The strain contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as the major polar lipids. The peptidoglycan structure was A3γ-type with LL-diaminopimelic acid. Based on these data, the isolate represents one novel species in the genus Nocardioides, for which the name Nocardioides paucivorans sp. nov. (type strain KIS31-44(T) =DSM 27142(T) =KACC 17309(T)) is proposed.
authors.affiliation: Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Wanju-gun, 565-851, Republic of Korea.
authors.forename: Jae-Hyung
authors.initials: JH
authors.lastname: Ahn
authors.forename: Jun-Muk
authors.initials: JM
authors.lastname: Lim
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Jaekyeong
authors.initials: J
authors.lastname: Song
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.forename: Hang-Yeon
authors.initials: HY
authors.lastname: Weon
mesh_terms: D000192:Actinomycetales / Q000737:chemistry* / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002308:Cardiolipins / Q000032:analysis*; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000032:analysis*; D010716:Phosphatidylinositols / Q000032:analysis*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D002308:Cardiolipins; D005227:Fatty Acids; D010457:Peptidoglycan; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid