title: A survey of the use of soy in processed Turkish meat products and detection of genetic modification.
abstract: To screen for possible illegal use of soybeans in meat products, the performance characteristics of a commercial polymer chain reaction (PCR) kit for detection of soybean DNA in raw and cooked meat products were established. Minced chicken and beef products containing soybean at levels from 0.1% to 10.0% were analysed by real-time PCR to amplify the soybean lectin gene. The PCR method could reliably detect the addition of soybean at a level of 0.1%. A survey of 38 Turkish processed meat products found only six samples to be negative for the presence of soybean. In 32 (84%) positive samples, 13 (34%) contained levels of soy above 0.1%. Of soybean positive samples, further DNA analysis was conducted by real-time PCR to detect whether genetically modified (GM) soybean had been used. Of 32 meat samples containing soybean, two samples were positive for GM modification.
authors.affiliation: a A&T Food Laboratory , Mega Center No 29, Istanbul , Turkey.
authors.forename: Pelin
authors.initials: P
authors.lastname: Ulca
authors.forename: Handan
authors.initials: H
authors.lastname: Balta
authors.forename: Hamide Z
authors.initials: HZ
authors.lastname: Senyuva
mesh_terms: D000818:Animals; D002417:Cattle; D018744:DNA, Plant / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis* / Q000331:legislation & jurisprudence; D030841:Food, Genetically Modified; D006801:Humans; D008461:Meat Products / Q000032:analysis*; D037121:Plant Lectins / Q000235:genetics*; D030821:Plants, Genetically Modified; D011200:Poultry; D060888:Real-Time Polymerase Chain Reaction; D030262:Soybean Proteins / Q000235:genetics*; D013025:Glycine max / Q000235:genetics*; D014421:Turkey; D010835:Phytohemagglutinins
chemical_list: D018744:DNA, Plant; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins
keywords: GM soybean; GMO; PCR; Turkish meat products