title: Roseimaritima ulvae gen. nov., sp. nov. and Rubripirellula obstinata gen. nov., sp. nov. two novel planctomycetes isolated from the epiphytic community of macroalgae.
abstract: Four isolates, belonging to the deep-branching phylum Planctomycetes, were recovered from the biofilm of two marine macroalgae, Ulva sp. and Laminaria sp., from the Northern coast of Portugal. These strains were light pink- or red-pigmented; the cells were variable in shape and usually organized in rosettes. They had a dimorphic cell cycle with budding reproduction. The organisms were chemoheterotrophic, strictly aerobic and mesophilic. The 16S rRNA gene sequence analysis showed that the strains belong to the family Planctomycetaceae with Rhodopirellula as the closest genus. The isolates form two separate branches (strain LF1(T) forms one branch and the strains UC8(T), UF3 and UF42 form a second branch) clearly separated from Rhodopirellula baltica with 94.2% and 93.8% 16S rRNA gene sequence similarity, respectively. Based on differential characteristics that distinguish the novel genera from R. baltica, such as cell size and shape, ultrastructure, enzymatic activities, substrate utilization pattern, fatty acid composition, phospholipid profiles and phylogeny we propose that the isolates represent two novel genera of the order Planctomycetales, Roseimaritima ulvae gen. nov., sp. nov. (type strain is UC8(T)=DSM 25454(T)=LMG 27778(T)) and Rubripirellula obstinata gen. nov., sp. nov. (type strain is LF1(T)=LMG 27779(T)=CECT 8602(T)).
authors.affiliation: Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal; CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. Electronic address: joana.bondoso@fc.up.pt.
authors.forename: Joana
authors.initials: J
authors.lastname: Bondoso
authors.affiliation: Centro de Neurociências e Biologia Celular, Universidade de Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Luciana
authors.initials: L
authors.lastname: Albuquerque
authors.affiliation: Departamento de Ciências da Vida, Apartado 3046, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
authors.forename: M Fernanda
authors.initials: MF
authors.lastname: Nobre
authors.affiliation: CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal; Laboratório de Biologia Celular, Instituto de Ciências Biomédicas Abel Salazar, ICBAS, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Lobo-da-Cunha
authors.affiliation: Departamento de Ciências da Vida, Apartado 3046, Universidade de Coimbra, 3001-401 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
authors.affiliation: Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007 Porto, Portugal; CIMAR/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental - Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal.
authors.forename: Olga Maria
authors.initials: OM
authors.lastname: Lage
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D058889:Molecular Typing; D010802:Phylogeny; D061328:Planctomycetales / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D012624:Seaweed / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Biofilm; Macroalgae; New taxa; Planctomycetes