title: Differential expression analysis of miRNA in peripheral blood mononuclear cells of patients with non-segmental vitiligo.
abstract: Vitiligo is a common depigmentary skin disease that may follow a pattern of multifactorial inheritance. The essential factors of its immunopathogenesis is thought to be the selective destruction of melanocytes. As a new class of microregulators of gene expression, miRNA have been reported to play vital roles in autoimmune diseases, metabolic diseases and cancer. This study sought to characterize the different miRNA expression pattern in the peripheral blood mononuclear cells (PBMC) of patients with non-segmental vitiligo (NSV) and healthy individuals and to examine their direct responses to thymosin α1 (Tα1) treatment. The miRNA expression profile in the PBMC of patients with NSV was analyzed using Exiqon's miRCURY LNA microRNA Array. The differentially expressed miRNA were validated by real-time quantitative polymerase chain reaction. We found that the expression levels of miR-224-3p and miR-4712-3p were upregulated, and miR-3940-5p was downregulated in the PBMC. The common clinical immune modulator Tα1 changed the miRNA expression profile. Our analysis showed that differentially expressed miRNA were associated with the mechanism of immune imbalance of vitiligo and that Tα1 could play an important role in changing the expression of these miRNA in the PBMC of patients with NSV. This study provided further evidence that miRNA may serve as novel drug targets for vitiligo therapeutic evaluation.
authors.affiliation: Department of Dermatology, Qilu Hospital, Shandong University, Jinan, China; Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Keyu
authors.initials: K
authors.lastname: Wang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Liang
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Ningning
authors.initials: N
authors.lastname: Dang
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Yi
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002478:Cells, Cultured; D002648:Child; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000187:drug effects; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000494:pharmacology; D015854:Up-Regulation; D014820:Vitiligo / Q000235:genetics* / Q000276:immunology*; D055815:Young Adult
chemical_list: D000276:Adjuvants, Immunologic; D035683:MicroRNAs; D013947:Thymosin; D000077596:Thymalfasin
keywords: blood circulation; immune cells; miRNA; non-segmental vitiligo; peripheral blood mononuclear cells