title: [Autophagy and apoptosis of HeLa cells induced by recombinant human endostatin combined with hypoxia].
abstract: OBJECTIVE
  To observe the impact of recombinant human endostatin (rhES) combined with hypoxia on the autophagy and apoptosis of human cervical cancer HeLa cells.
  
  
  METHODS
  Under hypoxia, HeLa cells in logarithmic growth phase were treated with PBS, rhES, rhES combined with 3-methyladenine (3-MA, inhibitor of autophagy), respectively. Twenty-four hours later, cell apoptosis rate was detected using Hochest33528 staining and annexin V-FITC/PI staining and flow cytometry; the expressions of microtubule-associated protein light chain 3 (LC-3), Bcl-2, Bax proteins were determined using Western blotting.
  
  
  RESULTS
  Hochest33528 staining suggested that cells of the hypoxia plus rhES group showed nuclear fragmentation; Annexin V-FITC/PI staining combined with flow cytometry revealed that the apoptosis rates were respectively (2.94±0.45)%, (21.38±0.92)% and (6.87±0.58)% in the three groups. It was significantly higher in the hypoxia plus rhES group than the other two groups. Western blotting showed that the expression levels of LC3 (LC-3II) and Bax increased and Bcl-2 decreased in the hypoxia plus rhES group as compared with the other two groups.
  
  
  CONCLUSIONS
  Endostatin combined hypoxia can induce autophagy and apoptosis of HeLa cells.
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Affiliated to Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Affiliated to Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Tingjun
authors.initials: T
authors.lastname: Bao
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Affiliated to Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Hospital Affiliated to Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Nian
authors.initials: N
authors.lastname: Wang
authors.affiliation: Medical School, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Medical School, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Wensi
authors.initials: W
authors.lastname: Huang
authors.affiliation: Medical School, Jiangsu University, Zhenjiang 212001, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D015687:Cell Hypoxia; D043169:Endostatins / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000032:analysis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D011994:Recombinant Proteins / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000032:analysis
chemical_list: D043169:Endostatins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D011994:Recombinant Proteins; D051028:bcl-2-Associated X Protein