title: Improving the detection of IgM antibodies against glycolipids complexes of GM1 and Galactocerebroside in Multifocal Motor Neuropathy using glycoarray and ELISA assays.
abstract: Antibodies against complexes of GM1:GalC are detected in multifocal motor neuropathy. Previous studies used different techniques, explaining disparities in the results. Antibodies against GM1 and GM1:GalC with different proportions of GalC were measured with both glycoarray and ELISA in 20 multifocal motor neuropathies, and 45 controls. The 1:5 ratio and the 1:1 ratio of GM1:GalC (weight ratio) were respectively the most effective for glycoarray and for ELISA. Testing for anti-GM1:GalC antibodies increased the sensitivity from 40% with anti-GM1 antibodies to 65% with array and 60% with ELISA without loss in specificity (above 91%). Anti-GM1:GalC antibodies are effective biological tools to diagnose multifocal motor neuropathy.
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom; Referral Centre for ALS and Neuromuscular diseases, Nice, France. Electronic address: emilien.delmo@gmail.com.
authors.forename: Emilien
authors.initials: E
authors.lastname: Delmont
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Halstead
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom.
authors.forename: Francesc
authors.initials: F
authors.lastname: Galban-Horcajo
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom.
authors.forename: Denggao
authors.initials: D
authors.lastname: Yao
authors.affiliation: Referral Centre for ALS and Neuromuscular diseases, Nice, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Desnuelle
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom.
authors.forename: Hugh
authors.initials: H
authors.lastname: Willison
mesh_terms: D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005699:Galactosylceramides / Q000276:immunology*; D016537:Gangliosidosis, GM1 / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D011115:Polyneuropathies / Q000097:blood*; D012372:ROC Curve; D018709:Statistics, Nonparametric
chemical_list: D005699:Galactosylceramides; D007075:Immunoglobulin M; C002454:galactocerebroside
keywords: Anti-ganglioside antibodies; ELISA; Gangliosides complexes; Multifocal motor neuropathy