title: Methicillin-resistant Staphylococcus sp. colonizing health care workers of a cancer hospital.
abstract: The aim of the study was to analyze epidemiological and microbiological aspects of oral colonization by methicillin-resistant Staphylococcus of health care workers in a cancer hospital. Interview and saliva sampling were performed with 149 health care workers. Antimicrobial resistance was determined by disk diffusion and minimum inhibitory concentration. Polymerase Chain Reaction, Internal Transcribed Spacer-Polymerase Chain Reaction and Pulsed Field Gel Electrophoresis were performed for genotypic characterization of methicillin-resistant Staphylococcus. Risk factors were determined by logistic regression. Methicillin-resistant Staphylococcus colonization prevalence was 19.5%, denture wearing (p = 0.03), habit of nail biting (p = 0.04) and preparation and administration of antimicrobial (p = 0.04) were risk factors identified. All methicillin-resistant Staphylococcus were S. epidermidis, 94.4% of them had mecA gene. Closely related and indistinguishable methicillin-resistant S. epidermidis were detected. These results highlight that HCWs which have contact with patient at high risk for developing infections were identified as colonized by MRSE in the oral cavity, reinforcing this cavity as a reservoir of these bacteria and the risk to themselves and patients safety, because these microorganisms may be spread by coughing and talking.
authors.affiliation: Núcleo de Estudos e Pesquisa de Enfermagem em Prevenção e Controle de Infecções Relacionadas à Assistência à Saúde Faculdade de Enfermagem Universidade Federal de Goiás GoiâniaGO Brazil Núcleo de Estudos e Pesquisa de Enfermagem em Prevenção e Controle de Infecções Relacionadas à Assistência à Saúde, Faculdade de Enfermagem, Universidade Federal de Goiás, Goiânia, GO, Brazil..
authors.forename: Dayane de Melo
authors.initials: Dde M
authors.lastname: Costa
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás GoiâniaGO Brazil Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Kipnis
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás GoiâniaGO Brazil Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Lara Stefânia Netto de Oliveira
authors.initials: LS
authors.lastname: Leão-Vasconcelos
authors.affiliation: Secretaria Municipal de Saúde de Goiânia GoiâniaGO Brazil Secretaria Municipal de Saúde de Goiânia, Goiânia, GO, Brazil.
authors.forename: Larissa Oliveira
authors.initials: LO
authors.lastname: Rocha-Vilefort
authors.affiliation: Faculdade de Enfermagem Universidade Federal de Goiás GoiâniaGO Brazil Faculdade de Enfermagem, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Sheila Araújo
authors.initials: SA
authors.lastname: Telles
authors.affiliation: Instituto de Patologia Tropical e Saúde Pública Universidade Federal de Goiás GoiâniaGO Brazil Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Maria Cláudia Dantas Porfírio Borges
authors.initials: MC
authors.lastname: André
authors.affiliation: Faculdade de Enfermagem Universidade Federal de Goiás GoiâniaGO Brazil Faculdade de Enfermagem, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Anaclara Ferreira Veiga
authors.initials: AF
authors.lastname: Tipple
authors.affiliation: Secretaria Municipal de Saúde de Goiânia GoiâniaGO Brazil Secretaria Municipal de Saúde de Goiânia, Goiânia, GO, Brazil.
authors.forename: Ana Beatriz Mori
authors.initials: AB
authors.lastname: Lima
authors.affiliation: Secretaria Municipal de Saúde de Goiânia GoiâniaGO Brazil Secretaria Municipal de Saúde de Goiânia, Goiânia, GO, Brazil.
authors.forename: Nádia Ferreira Gonçalves
authors.initials: NF
authors.lastname: Ribeiro
authors.affiliation: Núcleo de Estudos e Pesquisa de Enfermagem em Prevenção e Controle de Infecções Relacionadas à Assistência à Saúde Faculdade de Enfermagem Universidade Federal de Goiás GoiâniaGO Brazil Núcleo de Estudos e Pesquisa de Enfermagem em Prevenção e Controle de Infecções Relacionadas à Assistência à Saúde, Faculdade de Enfermagem, Universidade Federal de Goiás, Goiânia, GO, Brazil..
authors.forename: Mayara Regina
authors.initials: MR
authors.lastname: Pereira
authors.affiliation: Faculdade de Enfermagem Universidade Federal de Goiás GoiâniaGO Brazil Faculdade de Enfermagem, Universidade Federal de Goiás, Goiânia, GO, Brazil.
authors.forename: Marinésia Aparecida
authors.initials: MA
authors.lastname: Prado-Palos
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002173:Cancer Care Facilities; D002353:Carrier State / Q000453:epidemiology* / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006282:Health Personnel; D006801:Humans; D007407:Interviews as Topic; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012307:Risk Factors; D012463:Saliva / Q000382:microbiology; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; C487495:MecA protein, Staphylococcus epidermidis
keywords: Staphylococcus; health personnel; hospital; methicillin resistance; occupational health; oncology service