title: ADP1 affects abundance and endocytosis of PIN-FORMED proteins in Arabidopsis.
abstract: Auxin, as a vital plant hormone, regulates almost every aspect of plant growth and development. We previously identified a dominant mutant, adp1-D, displaying loss of apical dominance. We also demonstrated that down-regulation of local auxin biosynthesis in adp1-D was responsible for the bushy phenotype of this mutant. Consistent with the reduction of local auxin biosynthesis, we recently discovered that protein abundance of PIN1, PIN3, and PIN7 was reduced in adp1-D without accompanying transcription level changes. Additionally, subcellular analysis revealed that over-expression of ADP1 inhibited endocytosis of PIN proteins. Taken together, we conclude that ADP1 regulates plant architecture through the fine-tuning of local auxin biosynthesis and through post-transcriptional regulation of auxin transporters.
authors.affiliation: a State Key Laboratory of Protein and Plant Gene Research; Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology; Peking-Tsinghua Center for Life Sciences; College of Life Sciences ; Peking University ; Beijing , People's Republic of China.
authors.forename: Jieru
authors.initials: J
authors.lastname: Li
authors.forename: Ruixi
authors.initials: R
authors.lastname: Li
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Jiang
authors.forename: Hongya
authors.initials: H
authors.lastname: Gu
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Qu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; C000588907:At4g29140 protein, Arabidopsis; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; D027701:Organic Cation Transport Proteins; C116363:PIN1 protein, Arabidopsis; C449300:PIN3 protein, Arabidopsis; C479554:PIN7 protein, Arabidopsis
keywords: PIN; auxin; auxin biosynthesis; auxin polar; branch pattern; transport