title: Synthesis of silver nanoparticles using the Streptomyces coelicolor klmp33 pigment: an antimicrobial agent against extended-spectrum beta-lactamase (ESBL) producing Escherichia coli.
abstract: The increasing emergence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (E. coli) occurred mainly due to continuous persistent exposure to antibiotics causing high morbidity and mortality so studies in controlling this infection are required. In the present investigation, we developed a synthesis for silver nanoparticles employing a pigment produced by Streptomyces coelicolor klmp33, and assessed the antimicrobial activity of these nanoparticles against ESBL producing E. coli. The ESBL producing E. coli were isolated from urine samples collected from the Gulbarga region in India. As can been seen from our studies, the silver nanoparticles having irregular shapes and size of 28-50 nm showed remarkable antimicrobial activity and moreover the synthesis time is just 20 min and thus the same can be used for formulating pharmaceutical remedies.
authors.affiliation: Department of Microbiology, Gulbarga University, Gulbarga 585106, Karnataka, India.
authors.forename: Deene
authors.initials: D
authors.lastname: Manikprabhu
authors.affiliation: Department of Microbiology, Gulbarga University, Gulbarga 585106, Karnataka, India. Electronic address: lingappak123@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Lingappa
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D010860:Pigments, Biological / Q000737:chemistry; D018030:Silver Compounds / Q000138:chemical synthesis* / Q000494:pharmacology; D048372:Streptomyces coelicolor / Q000737:chemistry*; D014556:Urine / Q000382:microbiology; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010860:Pigments, Biological; D018030:Silver Compounds; C405902:GES-1 beta-lactamase; D001618:beta-Lactamases
keywords: Antimicrobial activity; Escherichia coli; Silver nanoparticles; Streptomyces coelicolor klmp33