title: Technical note: A portable on-chip assay system for absorbance and plasmonic detection of protein hormone in milk.
abstract: This paper reports a portable device and method to extract and detect protein hormone in milk samples. Recombinant protein hormone spiked into milk samples was extracted by solid-phase extraction, and detection was carried out using the plasmonic property of gold nanoislands deposited on a glass substrate. Trace levels of hormone spiked in milk were analyzed by their optical absorbance property using a microfluidic chip. We built a portable assay system using disposable lab-on-chip devices. The proposed method is able to detect spiked recombinant protein hormone in milk at concentrations as low as 5ng/mL.
authors.affiliation: Optical-Bio Microsystems Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, Quebec, Canada H3G 1M8.
authors.forename: Jayan
authors.initials: J
authors.lastname: Ozhikandathil
authors.affiliation: Optical-Bio Microsystems Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, Quebec, Canada H3G 1M8.
authors.forename: Simona
authors.initials: S
authors.lastname: Badilescu
authors.affiliation: Optical-Bio Microsystems Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, Quebec, Canada H3G 1M8. Electronic address: pmuthu@alcor.concordia.ca.
authors.forename: Muthukumaran
authors.initials: M
authors.lastname: Packirisamy
mesh_terms: D000818:Animals; D002417:Cattle; D004209:Disposable Equipment; D005260:Female; D006728:Hormones / Q000737:chemistry*; D056656:Lab-On-A-Chip Devices / Q000662:veterinary*; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry*
chemical_list: D006728:Hormones; D011994:Recombinant Proteins
keywords: hormone; lab-on-chip; milk; plasmonic detection