title: Functional analysis of OsPGIP1 in rice sheath blight resistance.
abstract: As one of the most devastating diseases of rice, sheath blight causes severe rice yield loss. However, little progress has been made in rice breeding for sheath blight resistance. It has been reported that polygalacturonase inhibiting proteins can inhibit the degradation of the plant cell wall by polygalacturonases from pathogens. Here, we prokaryotically expressed and purified OsPGIP1 protein, which was verified by Western blot analysis. Activity assay confirmed the inhibitory activity of OsPGIP1 against the PGase from Rhizoctonia solani. In addition, the location of OsPGIP1 was determined by subcellular localization. Subsequently, we overexpressed OsPGIP1 in Zhonghua 11 (Oryza sativa L. ssp. japonica), and applied PCR and Southern blot analysis to identify the positive T0 transgenic plants with single-copy insertions. Germination assay of the seeds from T1 transgenic plants was carried out to select homozygous OsPGIP1 transgenic lines, and the expression levels of OsPGIP1 in these lines were analyzed by quantitative real-time PCR. Field testing of R. solani inoculation showed that the sheath blight resistance of the transgenic rice was significantly improved. Furthermore, the levels of sheath blight resistance were in accordance with the expression levels of OsPGIP1 in the transgenic lines. Our results reveal the functions of OsPGIP1 and its resistance mechanism to rice sheath blight, which will facilitate rice breeding for sheath blight resistance.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Liaoxun
authors.initials: L
authors.lastname: Lu
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Pan
authors.forename: Zongliang
authors.initials: Z
authors.lastname: Hu
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.forename: Yemao
authors.initials: Y
authors.lastname: Liu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Lin
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D012232:Rhizoctonia / Q000472:pathogenicity*
chemical_list: D017931:DNA Primers; C081479:PGIP protein, plant; D010940:Plant Proteins