title: Genetic targeting of chemical indicators in vivo.
abstract: Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.
authors.affiliation: 1] Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy. [2] Molecular Medicine Partnership Unit (MMPU), Heidelberg, Germany.
authors.forename: Guoying
authors.initials: G
authors.lastname: Yang
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Fernanda
authors.initials: F
authors.lastname: de Castro Reis
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Mayya
authors.initials: M
authors.lastname: Sundukova
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Sofia
authors.initials: S
authors.lastname: Pimpinella
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Antonino
authors.initials: A
authors.lastname: Asaro
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Castaldi
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Batti
authors.affiliation: Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
authors.forename: Daniel
authors.initials: D
authors.lastname: Bilbao
authors.affiliation: 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.affiliation: 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
authors.affiliation: 1] Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy. [2] Molecular Medicine Partnership Unit (MMPU), Heidelberg, Germany.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Heppenstall
mesh_terms: D000818:Animals; D016326:Extracellular Matrix Proteins / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D055879:Gene Knock-In Techniques / Q000379:methods*; D017930:Genes, Reporter; D019426:Integrases / Q000235:genetics; D008822:Mice, Transgenic; D019853:O(6)-Methylguanine-DNA Methyltransferase / Q000737:chemistry / Q000235:genetics; D008249:Protein-Lysine 6-Oxidase / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D013194:Staining and Labeling
chemical_list: D016326:Extracellular Matrix Proteins; D005456:Fluorescent Dyes; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D022661:RNA, Untranslated; D011993:Recombinant Fusion Proteins; C077112:Lox protein, mouse; D008249:Protein-Lysine 6-Oxidase; D019853:O(6)-Methylguanine-DNA Methyltransferase; C045073:Cre recombinase; D019426:Integrases