title: Tools and methods to analyze autophagy in C. elegans.
abstract: For a long time, autophagy has been mainly studied in yeast or mammalian cell lines, and assays for analyzing autophagy in these models have been well described. More recently, the involvement of autophagy in various physiological functions has been investigated in multicellular organisms. Modification of autophagy flux is involved in developmental processes, resistance to stress conditions, aging, cell death and multiple pathologies. So, the use of animal models is essential to understand these processes in the context of different cell types and during the whole life. For ten years, the nematode Caenorhabditis elegans has emerged as a powerful model to analyze autophagy in physiological or pathological contexts. In this article, we present some of the established approaches and the emerging tools available to monitor and manipulate autophagy in C. elegans, and discuss their advantages and limitations.
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris Sud, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jenzer
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris Sud, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France.
authors.forename: Elena
authors.initials: E
authors.lastname: Simionato
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 1 Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris Sud, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France. Electronic address: renaud.legouis@cgm.cnrs-gif.fr.
authors.forename: Renaud
authors.initials: R
authors.lastname: Legouis
mesh_terms: D000375:Aging / Q000235:genetics* / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001343:Autophagy / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans
chemical_list: D029742:Caenorhabditis elegans Proteins
keywords: Aggrephagy; Aging; Allophagy; Cell death; Development; Pathologies