title: Transient expression using agroinfiltration to study polyadenylation in plants.
abstract: An important facet of the study of polyadenylation in plants entails the use of transient expression to characterize poly(A) sites and the subunits of the polyadenylation complex. In this report, a simple and adaptable approach to transient expression in Nicotiana benthamiana leaves is described. This approach has been used to evaluate protein-protein interactions amongst different plant polyadenylation factor subunits and to study the usage and behavior of individual poly(A) sites from plant genes.
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, 105 Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546, USA.
authors.forename: Carol
authors.initials: C
authors.lastname: Von Lanken
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Hunt
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics / Q000254:growth & development; D005822:Genetic Vectors; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D026723:Polyadenylation; D018749:RNA, Plant / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D014162:Transfection; D014170:Transformation, Genetic
chemical_list: D018749:RNA, Plant