title: SERS-based monitoring of the intracellular pH in endothelial cells: the influence of the extracellular environment and tumour necrosis factor-α.
abstract: The intracellular pH plays an important role in various cellular processes. In this work, we describe a method for monitoring of the intracellular pH in endothelial cells by using surface enhanced Raman spectroscopy (SERS) and 4-mercaptobenzoic acid (MBA) anchored to gold nanoparticles as pH-sensitive probes. Using the Raman microimaging technique, we analysed changes in intracellular pH induced by buffers with acid or alkaline pH, as well as in endothelial inflammation induced by tumour necrosis factor-α (TNFα). The targeted nanosensor enabled spatial pH measurements revealing distinct changes of the intracellular pH in endosomal compartments of the endothelium. Altogether, SERS-based analysis of intracellular pH proves to be a promising technique for a better understanding of intracellular pH regulation in various subcellular compartments.
authors.affiliation: Faculty of Chemistry, Jagiellonian University, 3 Ingardena Str., 30-060 Krakow, Poland. malek@chemia.uj.edu.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jaworska
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Jamieson
authors.forename: Kamilla
authors.initials: K
authors.lastname: Malek
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Campbell
authors.forename: Jaebum
authors.initials: J
authors.lastname: Choo
authors.forename: Stefan
authors.initials: S
authors.lastname: Chlopicki
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Baranska
mesh_terms: D001565:Benzoates / Q000737:chemistry; D005110:Extracellular Space / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000737:chemistry* / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry; D057054:Molecular Imaging; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D001565:Benzoates; D013438:Sulfhydryl Compounds; D014409:Tumor Necrosis Factor-alpha; C013594:4-mercaptobenzoate; D006046:Gold