title: Dextran grafting on PTFE surface for cardiovascular applications.
abstract: The modification of biomaterial surfaces with biomolecules influences the biological response. In this work, caboxymethyldextrans (CMD) with different degrees of substitution have been grafted to surfaces by introduction of amino moieties directly onto the substrate surface. Polytetrafluoroethylene was selected as a model substrate for biomaterial as it is already largely used for cardiovascular clinical applications. Firstly, CMD polymers were characterized by FTIR, (1)H-NMR, and conductimetric titration. Then, the coatings have been analyzed by XPS to confirm the grafting and determine the composition. Once characterized, biological performances of CMD coatings were investigated. The hemocompatibility was ascertained using the free hemoglobin method. The effects on endothelial and smooth muscle cell adhesion were also studied. Results indicated that CMD at a 0.2 substitution degree, significantly influenced the biological property of PTFE by exhibiting non-thrombogenic properties as well as enhancing endothelial cell adhesion along with limiting smooth muscle cell adhesion. This work suggested the creation of versatile pro-active biomaterials suitable for different biomedical applications.
authors.affiliation: a Laboratory for Biomaterials & Bioengineering (CRC-I); Dept Min-Met-Materials Eng. & CHU de Quebec Research Center; Laval University; Quebec City, QC Canada.
authors.forename: Eléonore C
authors.initials: EC
authors.lastname: Michel
authors.forename: Vanessa
authors.initials: V
authors.lastname: Montaño-Machado
authors.forename: Pascale
authors.initials: P
authors.lastname: Chevallier
authors.forename: Amélie
authors.initials: A
authors.lastname: Labbé-Barrère
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002318:Cardiovascular Diseases / Q000628:therapy; D002448:Cell Adhesion; D002460:Cell Line; D003911:Dextrans / Q000737:chemistry*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D011138:Polytetrafluoroethylene / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D011138:Polytetrafluoroethylene; C014392:carboxymethyl dextran
keywords: anti-thrombogenic property; carboxymethyldextran; dextran coatings; endothelial cells; smooth muscle cells; surface immobilization