title: A method for selecting modified DNAzymes without the use of modified DNA as a template in PCR.
abstract: Modified DNAzyme selections typically depend on recopying catalytically active modified DNA (mDNA) into cDNA in a PCR amplification step. However mDNA is often a poor template in PCR. Herein we propose a selection method in which the catalytically active, mDNA strand is covalently linked to the unmodified DNA template strand from which it was polymerized. Following selection, the unmodified DNA template is amplified in a PCR instead of the mDNA. This method circumvents the PCR amplification of mDNA.
authors.affiliation: Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver BC, V6T 1Z1, Canada. dperrin@chem.ubc.ca.
authors.forename: Marleen
authors.initials: M
authors.lastname: Renders
authors.forename: Emily
authors.initials: E
authors.lastname: Miller
authors.forename: Marcel
authors.initials: M
authors.lastname: Hollenstein
authors.forename: David
authors.initials: D
authors.lastname: Perrin
mesh_terms: D055162:Biocatalysis; D021881:DNA, Catalytic / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000235:genetics / Q000378:metabolism
chemical_list: D021881:DNA, Catalytic; D012313:RNA