title: Green synthesis and characterization of silver nanoparticles using alcoholic flower extract of Nyctanthes arbortristis and in vitro investigation of their antibacterial and cytotoxic activities.
abstract: Here we report the synthesis of silver nanoparticles using ethanolic flower extract of Nyctanthes arbortristis, UVvisible spectra and TEM indicated the successful formation of silver nanoparticles. Crystalline nature of the silver nanoparticles was confirmed by X-ray diffraction. Fourier Transform Infra-Red Spectroscopy analysis established the capping of the synthesized silver nanoparticles with phytochemicals naturally occurring in the ethanolic flower extract of N. arbortristis. The synthesized silver nanoparticles showed antibacterial activity against the pathogenic strain of Escherichia coli MTCC 443. Furthermore, cytotoxicity of the silver nanoparticles was tested on mouse fibroblastic cell line (L929) and found to be non-toxic, which thus proved their biocompatibility. Antibacterial activity and cytotoxicity assay carried out in this study open up an important perspective of the synthesized silver nanoparticles.
authors.affiliation: Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Nayanmoni
authors.initials: N
authors.lastname: Gogoi
authors.affiliation: Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Punuri Jayasekhar
authors.initials: PJ
authors.lastname: Babu
authors.affiliation: Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Department of Civil Engineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.
authors.forename: Chandan
authors.initials: C
authors.lastname: Mahanta
authors.affiliation: Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India; Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address: ubora@iitg.ernet.in.
authors.forename: Utpal
authors.initials: U
authors.lastname: Bora
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002460:Cell Line; D000431:Ethanol / Q000737:chemistry; D066298:In Vitro Techniques; D053768:Metal Nanoparticles; D051379:Mice; D046529:Microscopy, Electron, Transmission; D029592:Oleaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D000431:Ethanol; D012834:Silver
keywords: Antibacterial; Cell viability; Crystal growth of silver nanoparticles; Electron microscopy; FTIR; Nanocrystalline materials; Nyctanthes arbortristis; Spectroscopy