title: High pressure treatments combined with sodium lactate to inactivate Escherichia coli O157:H7 and spoilage microbiota in cured beef carpaccio.
abstract: High-pressure treatments (400 and 600 MPa) combined with the addition of sodium lactate (1 and 3%) were tested to reduce Escherichia coli O157:H7 (STEC O157) and spoilage microbiota contamination in a manufactured cured beef carpaccio in fresh or frozen conditions. Counts of spoilage microorganisms and STEC O157 were also examined during the curing step to prepare the carpaccio. STEC O157 counts remained almost unchanged through the curing process performed at 1 ± 1 °C for 12 days, with a small decrease in samples with 3% of sodium lactate. High-pressure treatments at 600 MPa for 5 min achieved an immediate reduction of up to 2 logarithmic units of STEC O157 in frozen carpaccio, and up to 1.19 log in fresh condition. Counts of spoilage bacteria diminished below detection limits in fresh or frozen carpaccio added with sodium lactate by the application of 400 and 600 MPa. Maximum injury on STEC O157 cells was observed at 600 MPa in carpaccio in fresh condition without added sodium lactate. Lethality of high-pressure treatments on STEC O157 was enhanced in frozen carpaccio, while the addition of sodium lactate at 3% reduced the lethality on STEC O157 in frozen samples, and the degree of injury in fresh carpaccio.
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), CC. 77, B1708WAB, Morón, Pcia. de Buenos Aires, Argentina. Electronic address: masana.marcelo@inta.gob.ar.
authors.forename: Marcelo Oscar
authors.initials: MO
authors.lastname: Masana
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), CC. 77, B1708WAB, Morón, Pcia. de Buenos Aires, Argentina; Departamento de Biotecnología y Tecnología Alimentaria, Facultad de Ingeniería y Ciencias Exactas, Universidad Argentina de la Empresa (UADE), Lima 717, C1073AAO, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Yanina Ximena
authors.initials: YX
authors.lastname: Barrio
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), CC. 77, B1708WAB, Morón, Pcia. de Buenos Aires, Argentina.
authors.forename: Pablo Martín
authors.initials: PM
authors.lastname: Palladino
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), CC. 77, B1708WAB, Morón, Pcia. de Buenos Aires, Argentina.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Sancho
authors.affiliation: Instituto Tecnología de Alimentos, Centro de Investigación de Agroindustria, Instituto Nacional de Tecnología Agropecuaria (INTA), CC. 77, B1708WAB, Morón, Pcia. de Buenos Aires, Argentina; Departamento de Biotecnología y Tecnología Alimentaria, Facultad de Ingeniería y Ciencias Exactas, Universidad Argentina de la Empresa (UADE), Lima 717, C1073AAO, Ciudad Autónoma de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia 1917, C1033AAJ, Ciudad Autónoma de Buenos Aires, Argentina.
authors.forename: Sergio Ramón
authors.initials: SR
authors.lastname: Vaudagna
mesh_terms: D000818:Animals; D002417:Cattle; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development*; D005519:Food Preservation / Q000295:instrumentation / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D064307:Microbiota / Q000187:drug effects; D011312:Pressure; D019354:Sodium Lactate / Q000494:pharmacology*
chemical_list: D019354:Sodium Lactate
keywords: Carpaccio; High hydrostatic pressure; STEC O157; Sodium lactate; Spoilage microbiota