title: A proteomic analysis of transplanted liver in a rat model of chronic rejection.
abstract: BACKGROUND
  Chronic rejection (CR) is an important cause of liver allograft failure. In the latter condition, re-transplantation of the liver (ReLT) is the only option for survival. Unfortunately, with the current state of knowledge, it is difficult to diagnose and treat early CR.
  
  
  OBJECTIVE
  To explore the biomarkers of the chronic rejection in orthotopic liver transplantation (OLT).
  
  
  METHODS
  A rat model of chronic liver allograft rejection was established, and the differential protein expression in chronic allograft rejection (CR) was analyzed by iTRAQ-MALDI-TOF/TOF.
  
  
  RESULTS
  Expression of sixty-two proteins was found to be significantly changed in CR rats. In the present study, CLU, Lcn2 and Krt19 were identified and quantified as early and reliable biomarkers for chronic rejection.
  
  
  CONCLUSIONS
  Analysis of differential protein expression by iTRAQ-MALDI-TOF/TOF is a potentially effective method to help understand the mechanism of CR in orthotopic liver transplantation. The proteins CLU, Lcn2 and Krt19 might be potential prognostic markers for predicting chronic rejection after liver transplantation.
authors.affiliation: The First Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Xin-Hui
authors.initials: XH
authors.lastname: Huang
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liang
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Yong-Yi
authors.initials: YY
authors.lastname: Zeng
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Chuang
authors.initials: C
authors.lastname: Ma
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Wu
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Yun-Tong
authors.initials: YT
authors.lastname: Li
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Zeng
authors.affiliation: Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Electronic address: drjingfeng@sina.com.
authors.forename: Jing-Feng
authors.initials: JF
authors.lastname: Liu
mesh_terms: D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D006084:Graft Rejection / Q000378:metabolism*; D016031:Liver Transplantation; D008297:Male; D014176:Protein Biosynthesis; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley