title: Ebola virus infection induces irregular dendritic cell gene expression.
abstract: Filoviruses subvert the human immune system in part by infecting and replicating in dendritic cells (DCs). Using gene arrays, a phenotypic profile of filovirus infection in human monocyte-derived DCs was assessed. Monocytes from human donors were cultured in GM-CSF and IL-4 and were infected with Ebola virus Kikwit variant for up to 48 h. Extracted DC RNA was analyzed on SuperArray's Dendritic and Antigen Presenting Cell Oligo GEArray and compared to uninfected controls. Infected DCs exhibited increased expression of cytokine, chemokine, antiviral, and anti-apoptotic genes not seen in uninfected controls. Significant increases of intracellular antiviral and MHC I and II genes were also noted in EBOV-infected DCs. However, infected DCs failed to show any significant difference in co-stimulatory T-cell gene expression from uninfected DCs. Moreover, several chemokine genes were activated, but there was sparse expression of chemokine receptors that enabled activated DCs to home to lymph nodes. Overall, statistically significant expression of several intracellular antiviral genes was noted, which may limit viral load but fails to stop replication. EBOV gene expression profiling is of vital importance in understanding pathogenesis and devising novel therapeutic treatments such as small-molecule inhibitors.
authors.affiliation: 1 Entomology Department, Walter Reed Army Institute of Research , Silver Spring, Maryland.
authors.forename: Vanessa R
authors.initials: VR
authors.lastname: Melanson
authors.forename: Warren V
authors.initials: WV
authors.lastname: Kalina
authors.forename: Priscilla
authors.initials: P
authors.lastname: Williams
mesh_terms: D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D029043:Ebolavirus / Q000276:immunology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology*; D006801:Humans; D046228:Microarray Analysis