title: MicroRNA-206 regulates surfactant secretion by targeting VAMP-2.
abstract: Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UTR) luciferase assay. Mutations in the predicated miR-206 binding sites reduced the binding of miR-206 to the 3'-UTR of VAMP-2. miR-206 decreased the expression of VAMP-2 protein and decreased the lung surfactant secretion in alveolar type II cells. In conclusion, miR-206 regulates lung surfactant secretion by limiting the availability of VAMP-2 protein.
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States.
authors.forename: Honghao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States; Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK 73034, United States.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States.
authors.forename: Amarjit
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Gou
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States.
authors.forename: Narendranath Reddy
authors.initials: NR
authors.lastname: Chintagari
authors.affiliation: Lundberg-Kienlen Lung Biology and Toxicology Laboratory, Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 73034, United States; Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK 73034, United States. Electronic address: lin.liu@okstate.edu.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000502:physiology; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050988:Vesicle-Associated Membrane Protein 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D011663:Pulmonary Surfactants; C494807:VAMP2 protein, human; C494706:Vamp2 protein, rat; D050988:Vesicle-Associated Membrane Protein 2
keywords: Lung surfactant secretion; MicroRNA-206; Vesicle-associated membrane protein 2