title: In vivo evolution of metabolic pathways: Assembling old parts to build novel and functional structures.
abstract: In our recent article "In vivo evolution of metabolic pathways by homeologous recombination in mitotic cells" we proposed a useful alternative to directed evolution methods that permits the generation of yeast cell libraries containing recombinant metabolic pathways from counterpart genes. The methodology was applied to generate single mosaic genes and intragenic mosaic pathways. We used flavonoid metabolism genes as a working model to assembly and express evolved pathways in DNA repair deficient cells. The present commentary revises the principles of gene and pathway mosaicism and explores the scope and perspectives of our results as an additional tool for synthetic biology.
authors.affiliation: a EVIAGENICS ; Villejuif , France.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Luque
authors.forename: Sarra C
authors.initials: SC
authors.lastname: Sebai
authors.forename: Vincent
authors.initials: V
authors.lastname: Sauveplane
authors.forename: Odile
authors.initials: O
authors.lastname: Ramaen
authors.forename: Rudy
authors.initials: R
authors.lastname: Pandjaitan
mesh_terms: D019020:Directed Molecular Evolution / Q000379:methods*; D059765:Homologous Recombination; D060847:Metabolic Engineering / Q000379:methods*; D008938:Mitosis / Q000235:genetics*; D012441:Saccharomyces cerevisiae
keywords: DNA repair deficiency; OXA genes; flavonoids; homeologous/homologous recombination; in vivo evolution; metabolic engineering; mosaic genes and pathways; phenylpropanoids; yeast