title: A new study of the bacterial lipidome: HPTLC-MALDI-TOF imaging enlightening the presence of phosphatidylcholine in airborne Pseudomonas fluorescens MFAF76a.
abstract: Lipids are major functional components of bacterial cells that play fundamental roles in bacterial metabolism and the barrier function between cells and the environment. In an effort to investigate the bacterial lipidome, we adopted a protocol using MALDI-TOF MS imaging coupled to HPTLC to screen a large number of phospholipid classes in a short span of time. With this method, phospholipids of airborne Pseudomonas fluorescens MFAF76a were visualized and identified in sample extracts (measurement accuracy below 0.1 Da, phospholipid identification by means of four characteristic fragment peaks). Via this technique, the P. fluorescens lipidome was shown to comprise three major lipid classes: phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The protocol described herein is simple, rapid and effective for screening of bacterial phospholipid classes. The remarkable presence of a eukaryotic phospholipid, phosphatidylcholine, was observed in P. fluorescens MFAF76a. This lipid is known to play a role in bacteria-host interactions and had not been known to be found in P. fluorescens cells.
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment (LMSM) EA4312, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France; Aerothermic and Internal Combustion Engine Technological Research Center (CERTAM), 1 Rue Joseph Fourier, 76800 Saint Etienne du Rouvray, France. Electronic address: tatiana.kondakova@etu.univ-rouen.fr.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kondakova
authors.affiliation: Team Modified to Surface and Interface Analysis (SIMA), UMR 6014 COBRA, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France. Electronic address: nadine.merlet@univ-rouen.fr.
authors.forename: Nadine
authors.initials: N
authors.lastname: Merlet-Machour
authors.affiliation: Bruker AXS S.A.S., 77420 Champs-sur-Marne, France. Electronic address: manuel.chapelle@bruker.fr.
authors.forename: Manuel
authors.initials: M
authors.lastname: Chapelle
authors.affiliation: Aerothermic and Internal Combustion Engine Technological Research Center (CERTAM), 1 Rue Joseph Fourier, 76800 Saint Etienne du Rouvray, France. Electronic address: david.preterre@certam-rouen.com.
authors.forename: David
authors.initials: D
authors.lastname: Preterre
authors.affiliation: Aerothermic and Internal Combustion Engine Technological Research Center (CERTAM), 1 Rue Joseph Fourier, 76800 Saint Etienne du Rouvray, France. Electronic address: frederic.dionnet@certam-rouen.com.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Dionnet
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment (LMSM) EA4312, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France. Electronic address: marc.feuilloley@univ-rouen.fr.
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment (LMSM) EA4312, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France. Electronic address: nicole.orange@univ-rouen.fr.
authors.forename: Nicole
authors.initials: N
authors.lastname: Orange
authors.affiliation: Laboratory of Microbiology Signals and Microenvironment (LMSM) EA4312, Normandy Univ., Univ. Rouen, 55 rue St Germain, 27000 Evreux, France. Electronic address: cecile.poc@univ-rouen.fr.
authors.forename: Cécile
authors.initials: C
authors.lastname: Duclairoir Poc
mesh_terms: D000391:Air Microbiology; D002855:Chromatography, Thin Layer / Q000379:methods*; D010713:Phosphatidylcholines / Q000032:analysis*; D010714:Phosphatidylethanolamines / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D011551:Pseudomonas fluorescens / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids
keywords: HPTLC MALDI TOF MSI; Lipidomics; Mass spectrometry imaging; Phosphatidylcholine; Phospholipids; Pseudomonas fluorescens