title: RAB8A a new biomarker for endometrial cancer?
abstract: BACKGROUND
  We aimed to find different proteins between dendritic cells (DCs) and tumor antigen-pulsed DCs to help find a new biomarker for endometrial cancer (EC).
  
  
  METHODS
  Mononuclear cells were isolated from cord blood and induced to DCs by cytokines. A comparative proteomic analysis was performed on DCs and tumor lysate-pulsed DCs by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Differential proteins were identified by Western blot analysis.
  
  
  RESULTS
  The expression of Ras-related protein Rab-8A (RAB8A) was found to be different in the in two kinds of cells. This phenomenon was also proven in endometrial cancer tissues.
  
  
  CONCLUSIONS
  RAB8A might be a new biomarker for endometrial cancer. Using LC-MS/MS to perform a comparative proteomic analysis about DCs and tumor lysate-pulsed DCs may help us to find new biomarker of cancer.
authors.forename: Yachun
authors.initials: Y
authors.lastname: Bie
authors.affiliation: Department of Obstetrics and Gynecology, Beijing Chao-yang Hospital affiliated to Capital Medical University, No, 8 Gongti South Road, Beijing 100020, China. bjzhangzhy@sina.cn.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D002478:Cells, Cultured; D002853:Chromatography, Liquid; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D016889:Endometrial Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D008213:Lymphocyte Activation; D011379:Prognosis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C082377:RAB8A protein, human; D020691:rab GTP-Binding Proteins