title: Enhanced electrochemical sensing of thiols based on cobalt phthalocyanine immobilized on nitrogen-doped graphene.
abstract: A hybrid nanocomposite based on cobalt phthalocyanine (CoPc) immobilized on nitrogen-doped graphene (N-G) (N-G/CoPc) has been developed to modify glassy carbon electrode (GCE) for the sensitive detection of thiols. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and electrochemical impedance spectroscopy (EIS). Cyclic voltammetric studies showed that cobalt phthalocyanine and nitrogen doped graphene have a synergic effect and significantly enhance the electrocatalytic activity of the modified electrode towards thiols oxidation compared with electrodes modified with solely CoPc or N-G. The electrochemical oxidation responses were studied and the reaction mechanisms were discussed. The sensors exhibited a wide linear response range from 1μΜ to 16mM and a low detection limit of 1μΜ for the determination of l-cysteine, reduced l-glutathione and 2-mercaptoethanesulfonic acid in alkaline aqueous solution. The proposed N-G/CoPc hybrids contribute to the construction of rapid, convenient and low-cost electrochemical sensors for sensitive detection of thiols.
authors.affiliation: Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institute of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institute of Biomedical Sciences, Fudan University, Shanghai 200433, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institute of Biomedical Sciences, Fudan University, Shanghai 200433, PR China. Electronic address: bhliu@fudan.edu.cn.
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
authors.affiliation: PSL Research University, Chimie ParisTech, Unité de Technologies Chimiques et Biologiques pour la Santé, 75005 Paris, France; INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (n° 1022), 75005 Paris, France; CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, 75005 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, 75005 Paris, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Griveau
authors.affiliation: PSL Research University, Chimie ParisTech, Unité de Technologies Chimiques et Biologiques pour la Santé, 75005 Paris, France; INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (n° 1022), 75005 Paris, France; CNRS, Unité de Technologies Chimiques et Biologiques pour la santé UMR 8258, 75005 Paris, France; Université Paris Descartes, Sorbonne Paris Cité, Unité de Technologies Chimiques et Biologiques pour la Santé, 75005 Paris, France.
authors.forename: Fethi
authors.initials: F
authors.lastname: Bedioui
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D003545:Cysteine / Q000032:analysis; D055664:Electrochemical Techniques; D005978:Glutathione / Q000032:analysis; D006108:Graphite; D007211:Indoles; D057230:Limit of Detection; D015080:Mesna / Q000032:analysis; D053761:Nanocomposites; D009584:Nitrogen; D009942:Organometallic Compounds; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D007211:Indoles; D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds; C063633:cobalt phthalocyanine; D006108:Graphite; D005978:Glutathione; D003545:Cysteine; D009584:Nitrogen; D015080:Mesna
keywords: Cobalt phthalocyanine; Hybrid electrode; Nitrogen-doped graphene; Thiol oxidation