title: [Long-term expansion of multipotent mesenchymal stromal cells under reduced oxygen tension].
abstract: We have shown that the decrease in oxygen tension in the culture medium of multipotent mesenchymal stromal cells (MMSCs) results in a short-term reduction in the proportion of CD73(+)-cells in the population, without effecting the number of cells expressing other constitutive surface markers (CD90 and CD105). In this case, the heterogeneity of the cell population declined: large spread cells disappeared. The proliferative activity of MMSCs significantly increased and remained stable in conditions in which the oxygen content was close to the tissue oxygen levels (5% O2). At lower oxygen concentration, proliferative activity of the cells gradually reduced from passages 3-4. The increase in proliferative activity was not accompanied by increased expression of telomerase gene indicateding the alsance of cell transformation. However, genome-wide analysis of MMSC gene expression level revealed changes in expression of cyclins (CCND2 and PCNA), regulatory subunit cyclin-dependent kinase (CKS2) and an inhibitor of cyclin-dependent kinase (CDKN2C), regulating the cell cycle, which is obviously facilitated the increase in the proliferative capacity of cells at lower oxygen tension.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Rylova
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D015720:5'-Nucleotidase / Q000235:genetics / Q000276:immunology; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D003470:Culture Media; D000071063:Endoglin; D058851:GPI-Linked Proteins / Q000235:genetics / Q000276:immunology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000276:immunology; D019098:Telomerase / Q000096:biosynthesis; D018800:Thy-1 Antigens / Q000235:genetics / Q000276:immunology
chemical_list: D015703:Antigens, CD; D003470:Culture Media; C064206:ENG protein, human; D000071063:Endoglin; D058851:GPI-Linked Proteins; D011956:Receptors, Cell Surface; D018800:Thy-1 Antigens; C509186:TERT protein, human; D019098:Telomerase; D015720:5'-Nucleotidase; C548241:NT5E protein, human; D010100:Oxygen