title: [Effect of incubation in sodium chloride hypertonic solutions on human blood lymphocyte DNA damage formation by long-wave UV-radiation].
abstract: Effect of incubation in NaCl hypertonic solutions (0.2, 0.35 and 0.5 mol/L for 1 h at 4 degrees C) on the DNA single-strand breaks (SSB) and alkaline-labile site (ALS) yields induced by long-wave UV-radiation (365 +/- 10 nm) in human blood peripheral lymphocytes in vitro was investigated. It was shown that compared to the cells incubated in NaCl isotonic solution (0.14 mol/L) statistically significant increases in the yields of both spontaneous (-1.5-1.9 times) and UV-A radiation induced (-1.6-1.7 times) DNA damage was observed only at the NaCl concentration of 0.5 mol/L. It is assumed that at this concentration of NaCl, dissociation of the linker histone H1 occurs, the structure of chromatin is disrupted and the free radical-induced DNA damage output dramatically increases.
authors.forename: N M
authors.initials: NM
authors.lastname: Smetanina
authors.forename: M V
authors.initials: MV
authors.lastname: Pustovalova
authors.forename: A N
authors.initials: AN
authors.lastname: Osipov
mesh_terms: D000328:Adult; D002843:Chromatin / Q000187:drug effects* / Q000528:radiation effects*; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects; D006657:Histones / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000528:radiation effects*; D008297:Male; D012462:Saline Solution, Hypertonic / Q000494:pharmacology; D014466:Ultraviolet Rays
chemical_list: D002843:Chromatin; D006657:Histones; D012462:Saline Solution, Hypertonic