title: Comparative study of cultivation of feces in vermiculite or charcoal to obtain larvae of Strongyloides venezuelensis.
abstract: BACKGROUND
  We compared feces culturing in charcoal or vermiculite to obtain Strongyloides venezuelensis larvae.
  
  
  METHODS
  Feces (5 g) from infected rats was mixed with vermiculite (10 g) or coal (10 g) in plastic cups and incubated at 28°C for 48 h. Larvae were recovered using Baermann-Moraes method.
  
  
  RESULTS
  Significantly higher number of positive larval cultures were recovered from vermiculite than from charcoal (15/17 and 4/17, respectively; p < 0.001; 990.6 ± 307.5 and 215 ± 78.1 larvae, p = 0.027).
  
  
  CONCLUSIONS
  Vermiculite yields more larvae and provides cleaner pellets, improving larvae identification and facilitating their use for other purposes.
authors.affiliation: Programa Pós-Graduação em Doenças Infecciosas, Núcleo de Doenças Infecciosas, Universidade Federal de Espírito Santo, Vitória, ES, Brazil.
authors.forename: Steveen Rios
authors.initials: SR
authors.lastname: Ribeiro
authors.affiliation: Programa Pós-Graduação em Doenças Infecciosas, Núcleo de Doenças Infecciosas, Universidade Federal de Espírito Santo, Vitória, ES, Brazil.
authors.forename: Caroline Ohnesorge
authors.initials: CO
authors.lastname: Maia
authors.affiliation: Programa Pós-Graduação em Doenças Infecciosas, Núcleo de Doenças Infecciosas, Universidade Federal de Espírito Santo, Vitória, ES, Brazil.
authors.forename: Fausto Edmundo Lima
authors.initials: FE
authors.lastname: Pereira
authors.affiliation: Departamento de Patologia, Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Narcisa Imaculada Brant
authors.initials: NI
authors.lastname: Moreira
mesh_terms: D000538:Aluminum Silicates / Q000494:pharmacology*; D000818:Animals; D002606:Charcoal / Q000494:pharmacology*; D003470:Culture Media; D005243:Feces / Q000469:parasitology*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D010270:Parasite Egg Count; D051381:Rats; D017208:Rats, Wistar; D013321:Strongyloides / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors
chemical_list: D000538:Aluminum Silicates; D003470:Culture Media; C003760:vermiculite; D002606:Charcoal