title: Alveolar bone regeneration in response to local application of calcitriol in vitamin D deficient rats.
abstract: OBJECTIVE
  Vitamin D deficiency is considered to diminish bone regeneration. Yet, raising the serum levels takes months. A topic application of the active vitamin D metabolite, calcitriol, may be an effective approach. Thus, it becomes important to know the effect of vitamin D deficiency and local application on alveolar bone regeneration.
  
  
  METHODS
  Sixty rats were divided into three groups; two vitamin depletion groups and a control group. Identical single defects (2 mm diameter) were created in the maxilla and mandible treated with calcitriol soaked collagen in one deficiency group while in the other two groups not. Histomorphometric analysis and micro CTs were performed after 1 and 3 weeks. Serum levels of 25(OH)D3 and PTH were determined.
  
  
  RESULTS
  Bone formation rate significantly increased within the observation period in all groups. Bone regeneration was higher in the maxilla than in the mandible. However, bone regeneration was lower in the control group compared to vitamin depletion groups, with no significant effects by local administration of calcitriol (micro CT mandible p = 0.003, maxilla p < 0.001; histomorphometry maxilla p = 0.035, mandible p = 0.18).
  
  
  CONCLUSIONS
  Vitamin D deficiency not necessarily impairs bone regeneration in the rat jaw and a single local calcitriol application does not enhance healing.
authors.affiliation: Department of Oral Surgery, Medical University of Vienna, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Alexander
authors.initials: A
authors.lastname: Fügl
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gruber
authors.forename: Hermann
authors.initials: H
authors.lastname: Agis
authors.forename: Hannah
authors.initials: H
authors.lastname: Lzicar
authors.forename: Claudia
authors.initials: C
authors.lastname: Keibl
authors.forename: Uwe Yacine
authors.initials: UY
authors.lastname: Schwarze
authors.forename: Gabriella
authors.initials: G
authors.lastname: Dvorak
mesh_terms: D000287:Administration, Topical; D016301:Alveolar Bone Loss / Q000188:drug therapy* / Q000473:pathology; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage*; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D001861:Bone Regeneration / Q000187:drug effects*; D002112:Calcifediol / Q000097:blood; D002113:Calcification, Physiologic / Q000187:drug effects; D002117:Calcitriol / Q000008:administration & dosage*; D008297:Male; D008336:Mandibular Diseases / Q000188:drug therapy / Q000473:pathology; D008439:Maxillary Diseases / Q000188:drug therapy / Q000473:pathology; D009929:Organ Size; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D010281:Parathyroid Hormone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014808:Vitamin D Deficiency / Q000150:complications*; D055114:X-Ray Microtomography / Q000379:methods
chemical_list: D050071:Bone Density Conservation Agents; D010281:Parathyroid Hormone; D002117:Calcitriol; D002112:Calcifediol
keywords: 1,25(OH)D3; bone healing; calcitriol; supplementation; topic administration; vitamin D deficiency