title: Effects of polyunsaturated fatty acids on endometrial carcinoma Ishikawa cells.
abstract: OBJECTIVE
  This study aimed to explore the effects and mechanisms of polyunsaturated fatty acids in the endometrial carcinoma Ishikawa cells.
  
  
  METHODS
  The Ishikawa cells were treated with n-6 PUFAs, n-3 PUFAs, or the mixture of n-6/n-3 PUFAs at a ratio of 1:1/10:1. Cell proliferation was quantified by MTT assay, while expressions of Akt, mTOR and VEGF mRNAs by PCR.
  
  
  RESULTS
  Treatment with n-6 PUFAs and 10:1 n-6/n-3PUFAs markedly promoted cell proliferation and up-regulated expressions of Akt, mTOR and VEGF mRNAs. Further, n-3 PUFAs and 1:1 n-6/n-3 PUFAs markedly suppressed cell proliferation and down-regulated expressions of Akt, mTOR and VEGF mRNAs.
  
  
  CONCLUSIONS
  Different ratios of n-6/n-3PUFAs exhibit differential effects on the endometrial carcinoma Ishikawa cells through the PI3k/Akt/mTOR signaling pathway and differentially regulate VEGF expression.
authors.affiliation: Department of Obstetrics and Gynecology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, China.  xy_lu68@aliyun.com.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Lu
authors.forename: T
authors.initials: T
authors.lastname: Zeng
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005260:Female; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D014407:Tumor Cells, Cultured; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; D042461:Vascular Endothelial Growth Factor A; C467484:VEGFA protein, human; C546842:MTOR protein, human