title: Histiocytic typhlocolitis in two colony Beagle dogs.
abstract: Two young female Beagle dogs in a laboratory colony with clinical signs of loose stools and fecal blood were confirmed to have histiocytic ulcerative colitis by histologic evaluation. This syndrome is well recognized in other dog breeds such as Boxers and related French Bulldogs, Mastiffs, Alaskan malamutes and Doberman Pinschers. Formalin-fixed paraffin sections of large intestine from one dog demonstrated the presence of Escherichia coli strain LF82 by immunohistochemistry and 16S ribosomal RNA gene sequencing. E coli strain LF82 has been implicated in the pathogenesis of Crohn's disease and similar bacteria have been cultured from cases of histiocytic ulcerative colitis in Boxer dogs. Spontaneous histiocytic ulcerative colitis must be differentiated from test article-related findings in nonclinical toxicity studies in Beagle dogs.
authors.affiliation: California Animal Health and Food Safety Laboratory, 105 West Central Avenue, San Bernardino, CA 92408, USA.
authors.forename: Francisco R
authors.initials: FR
authors.lastname: Carvallo
authors.affiliation: Drug Safety, Research and Development, Pfizer Inc., 445 Eastern Point Road, MS 8274-1221, Groton, CT 06340, USA.
authors.forename: Roy
authors.initials: R
authors.lastname: Kerlin
authors.affiliation: Pharmatherapeutics Research, Worldwide Research and Development, Pfizer Inc., 445 Eastern Point Road, MS 8274-1253, Groton, CT 06340, USA.
authors.forename: Claudine
authors.initials: C
authors.lastname: Fredette
authors.affiliation: The Research Institute, Nationwide Children's Hospital, 700 Children's Drive, Columbus, OH, USA.
authors.forename: Harshan
authors.initials: H
authors.lastname: Pisharath
authors.affiliation: The E. coli Reference Center, The Pennsylvania State University, University Park, PA, USA.
authors.forename: Chitrita
authors.initials: C
authors.lastname: DebRoy
authors.affiliation: Animal Diagnostic Laboratory, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Subhashinie
authors.initials: S
authors.lastname: Kariyawasam
authors.affiliation: Drug Safety, Research and Development, Pfizer Inc., 445 Eastern Point Road, MS 8274-1221, Groton, CT 06340, USA. Electronic address: ingrid.d.pardo@pfizer.com.
authors.forename: Ingrid D
authors.initials: ID
authors.lastname: Pardo
mesh_terms: D000818:Animals; D002432:Cecum / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000382:microbiology / Q000473:pathology; D003424:Crohn Disease / Q000382:microbiology / Q000473:pathology* / Q000662:veterinary*; D004283:Dog Diseases / Q000382:microbiology / Q000473:pathology*; D004285:Dogs; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology* / Q000662:veterinary*; D005260:Female; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology
keywords: Crohn's disease; Escherichia coli strain LF82; Granulomatous colitis; Ulcerative colitis