title: A rapid, simple, and inexpensive method for the preparation of strand-specific RNA-Seq libraries.
abstract: High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.
authors.affiliation: Department of Plant and Soil Sciences, University of Kentucky, 301A Plant Science Building, 1405 Veterans Drive, Lexington, KY, 40546-0312, USA, aghunt00@uky.edu.
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Hunt
mesh_terms: D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D026723:Polyadenylation; D012333:RNA, Messenger / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger