title: Methods for sample acquisition and processing of serial blood and tumor biopsies for multicenter diffuse large B-cell lymphoma clinical trials.
abstract: Increasingly, targeted therapies are being developed to treat malignancies. To define targets, determine mechanisms of response and resistance, and develop biomarkers for the successful investigation of novel therapeutics, high-quality tumor biospecimens are critical. We have developed standard operating procedures (SOPs) to acquire and process serial blood and tumor biopsies from patients with diffuse large B-cell lymphoma enrolled in multicenter clinical trials. These SOPs allow for collection and processing of materials suitable for multiple downstream applications, including immunohistochemistry, cDNA microarrays, exome sequencing, and metabolomics. By standardizing these methods, we control preanalytic variables that ensure high reproducibility of results and facilitate the integration of datasets from such trials. This will facilitate translational research, better treatment selection, and more rapid and efficient development of new drugs. See all the articles in this CEBP Focus section, "Biomarkers, Biospecimens, and New Technologies in Molecular Epidemiology."
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada.
authors.forename: Torsten Holm
authors.initials: TH
authors.lastname: Nielsen
authors.affiliation: Quebec Clinical Research Organization in Cancer, Montreal, Quebec, Canada.
authors.forename: Zuanel
authors.initials: Z
authors.lastname: Diaz
authors.affiliation: Clinical Research Unit, Jewish General Hospital, Montreal, McGill University, Quebec, Canada.
authors.forename: Rosa
authors.initials: R
authors.lastname: Christodoulopoulos
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada.
authors.forename: Fredrick
authors.initials: F
authors.lastname: Charbonneau
authors.affiliation: Quebec Clinical Research Organization in Cancer, Montreal, Quebec, Canada.
authors.forename: Samia
authors.initials: S
authors.lastname: Qureshi
authors.affiliation: Quebec Clinical Research Organization in Cancer, Montreal, Quebec, Canada.
authors.forename: Caroline
authors.initials: C
authors.lastname: Rousseau
authors.affiliation: Department of Pathology, Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
authors.forename: Naciba
authors.initials: N
authors.lastname: Benlimame
authors.affiliation: Department of Radiology, Jewish General Hospital, Montreal, McGill University, Quebec, Canada.
authors.forename: Errol
authors.initials: E
authors.lastname: Camlioglu
authors.affiliation: Department of Radiology, Jewish General Hospital, Montreal, McGill University, Quebec, Canada.
authors.forename: André Marc
authors.initials: AM
authors.lastname: Constantin
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada.
authors.forename: Kathleen Klein
authors.initials: KK
authors.lastname: Oros
authors.affiliation: Institute of Computational Biology, Helmholtz Zentrum München, Germany. Division of Hematology and Oncology, Department of Medicine, Cornell University, New York, New York.
authors.forename: Jan
authors.initials: J
authors.lastname: Krumsiek
authors.affiliation: Princess Margaret Hospital, Toronto, Ontario, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: Crump
authors.affiliation: Department of Molecular Biology and Biochemistry, Simon Frasier University, Burnaby, British Columbia, Canada.
authors.forename: Ryan D
authors.initials: RD
authors.lastname: Morin
authors.affiliation: Division of Hematology and Oncology, Department of Medicine, Cornell University, New York, New York.
authors.forename: Leandro
authors.initials: L
authors.lastname: Cerchietti
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada. Department of Hematology, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. Department of Oncology, McGill University, Quebec, Canada.
authors.forename: Nathalie A
authors.initials: NA
authors.lastname: Johnson
authors.affiliation: Department of Pathology, Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
authors.forename: Tina
authors.initials: T
authors.lastname: Petrogiannis-Haliotis
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada. Department of Oncology, McGill University, Quebec, Canada.
authors.forename: Wilson H
authors.initials: WH
authors.lastname: Miller
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada. Department of Hematology, Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
authors.forename: Sarit E
authors.initials: SE
authors.lastname: Assouline
authors.affiliation: Lady Davis Institute for Medical Research, McGill University, Quebec, Canada. Department of Oncology, McGill University, Quebec, Canada. koren.mann@mcgill.ca.
authors.forename: Koren K
authors.initials: KK
authors.lastname: Mann
mesh_terms: D005260:Female; D006801:Humans; D008297:Male; D001706:Biopsy / Q000379:methods; D016393:Lymphoma, B-Cell / Q000175:diagnosis; D055432:Metabolomics; D009369:Neoplasms / Q000097:blood / Q000601:surgery; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods