title: Antiosteoporotic effect of combined extract of Morus alba and Polygonum odoratum.
abstract: Due to the limitation of osteoporosis therapy, the alternative therapies from natural sources have been considered. In this study, we aimed to determine the antiosteoporotic effect of the combined extract of Morus alba and Polygonum odoratum leaves. Ovariectomized rats, weighing 200-220 g, were orally given the combined extract at doses of 5, 150, and 300 mg·kg(-1) BW for 3 months. At the end of study, blood was collected to determine serum osteocalcin, calcium, and alkaline phosphatase level. In addition, tibia bone was isolated to determine bone oxidative stress markers, cortical bone thickness, and density of osteoblast. The combined extract decreased oxidative stress and osteoclast density but increased osteoblast density and cortical thickness. The elevation of serum calcium, alkaline phosphatase, and osteocalcin was also observed. These results suggested the antiosteoporotic effect of the combined extract via the increased growth formation together with the suppression of bone resorption. However, further studies concerning chronic toxicity and the underlying mechanism are required.
authors.affiliation: Department of Physiology and Graduate School (Neuroscience Program), Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand ; Integrated Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40000, Thailand.
authors.forename: Sudarat
authors.initials: S
authors.lastname: Sungkamanee
authors.affiliation: Integrated Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40000, Thailand ; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand.
authors.forename: Jintanaporn
authors.identifier: 0000-0002-7383-2348
authors.initials: J
authors.lastname: Wattanathorn
authors.affiliation: Integrated Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40000, Thailand ; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand.
authors.forename: Supaporn
authors.identifier: 0000-0001-7756-1955
authors.initials: S
authors.lastname: Muchimapura
authors.affiliation: Integrated Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen 40000, Thailand ; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40000, Thailand.
authors.forename: Wipawee
authors.initials: W
authors.lastname: Thukham-mee
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000188:drug therapy; D005260:Female; D031625:Morus / Q000737:chemistry*; D010024:Osteoporosis / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D028502:Polygonum / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts