title: Enzyme-free and label-free fluorescence sensor for the detection of liver cancer related short gene.
abstract: Non-invasive early diagnosis of liver cancer is the most effective way to improve the survival rate. In this paper, we developed a label-free and enzyme-free fluorescent biosensor based on target recycling and Thioflavin T (ThT) induced G-quadruplex formation for MXR7 (liver cancer related short gene) detection in human serum. The proposed sensor can detect the target DNA in the concentration range of 0-350fM with the detection limit as low as 10fM. Due to the outstanding structural selectivity of ThT for G-quadruplex, this sensor possesses better discrimination ability and higher sensitivity. Furthermore, this enzyme-free and label-free fluorescence sensor has demonstrated to be capable of detecting target DNA in human serum samples because of its high selectivity and sensitivity. The mechanism employed in this study represents a promising path toward directing liver cancer detection in human serum. In addition, this strategy may be extended to detect other cancer related genes by choosing a rational DNA probe according to different sequences of targets.
authors.affiliation: Medical Technology and Engineering College, Fujian Medical University, Fuzhou 350004, Fujian, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, The 1st Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou 350004, Fujian, China.
authors.forename: Longjie
authors.initials: L
authors.lastname: Gan
authors.affiliation: Medical Technology and Engineering College, Fujian Medical University, Fuzhou 350004, Fujian, China; Department of Laboratory Medicine, The 1st Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou 350004, Fujian, China.
authors.forename: Qishui
authors.initials: Q
authors.lastname: Ou
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China.
authors.forename: Shuxian
authors.initials: S
authors.lastname: Cai
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China.
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China.
authors.forename: Yaokun
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou 350108, Fujian, China. Electronic address: cjh_huaxue@126.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The 1st Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou 350004, Fujian, China. Electronic address: yangbin607@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D052160:Benzothiazoles; D004247:DNA / Q000737:chemistry; D054856:G-Quadruplexes; D053673:Glypicans / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D008113:Liver Neoplasms / Q000097:blood*; D013050:Spectrometry, Fluorescence; D013844:Thiazoles / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; C105937:GPC3 protein, human; D053673:Glypicans; D013844:Thiazoles; C009462:thioflavin T; D004247:DNA
keywords: Enzyme-free; Fluorescence sensor; G-quadruplex; Label-free; Thioflavine T