title: Total synthesis of elastin peptide using high pressure-liquid phase synthesis assisted by a soluble tag strategy.
abstract: A highly aggregating elastin peptide was prepared efficiently using a high pressure-liquid phase synthesis approach assisted by a soluble tag strategy. Two standard syringes were connected to each other to construct a reactor. This simple reactor was used to apply high pressure to the highly viscous reaction mixture thereby maintaining its fluidity. The reactions were completely inhibited due to aggregation when conducted in a standard flask reactor, whereas our high pressure approach accelerated the couplings to realize complete conversion within 5-7 min. All steps were conducted at 0.10 M concentration, affording grams of the desired product.
authors.affiliation: Department of Applied Biological Chemistry, Tokyo University of Agriculture and Technology , 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Okada
authors.forename: Shoichi
authors.initials: S
authors.lastname: Hosoya
authors.forename: Hidenobu
authors.initials: H
authors.lastname: Suzuki
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
mesh_terms: D000595:Amino Acid Sequence; D004549:Elastin / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D010455:Peptides; D004549:Elastin