title: Linking eggs and adults of Argulus spp. using mitochondrial DNA barcodes.
abstract: We have created barcode library for common Argulus spp. infecting Carassius auratus, which could also be used to identify premature forms of Argulus spp. even by non-professionals. Infected C. auratus was examined and purchased from ornamental fish-trading centers and the adult life stage of Argulus spp. was identified and DNA barcoded. The eggs of Argulus spp. were collected using bottle implants. The collected eggs are barcoded and precisely identified by matching with the adult sequences. Four species of adult Argulus spp. were identified, namely Argulus japonicus, Argulus indicus, Argulus siamensis, and Argulus foliaceus. Precise identification of egg samples was done by two different analyses, namely (i) BLAST analysis and (ii) phylogenetic clustering of adults and eggs. All egg samples including the control were precisely identified by BLAST analysis and the results are consistent with phylogenetic clustering of adult and egg's DNA barcodes. In order to establish the DNA barcode technology for the identification of all Argulus spp and its premature forms, the development of full-fledged barcode library that includes all species of this genus is very important for the benefit of ornamental fish industries.
authors.affiliation: a Faculty of Marine Sciences , Centre of Advanced Study in Marine Biology, Annamalai University , Parangipettai , Tamil Nadu , India.
authors.forename: K
authors.initials: K
authors.lastname: Feroz Khan
authors.affiliation: a Faculty of Marine Sciences , Centre of Advanced Study in Marine Biology, Annamalai University , Parangipettai , Tamil Nadu , India.
authors.forename: G
authors.initials: G
authors.lastname: Sanker
authors.affiliation: a Faculty of Marine Sciences , Centre of Advanced Study in Marine Biology, Annamalai University , Parangipettai , Tamil Nadu , India.
authors.forename: C
authors.initials: C
authors.lastname: Prasanna Kumar
mesh_terms: D000818:Animals; D033341:Arguloida / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D016000:Cluster Analysis; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D058893:DNA Barcoding, Taxonomic; D004272:DNA, Mitochondrial / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D030541:Databases, Genetic; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D054629:Genome, Mitochondrial; D006054:Goldfish / Q000235:genetics / Q000469:parasitology; D010063:Ovum / Q000378:metabolism; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D004247:DNA; D003576:Electron Transport Complex IV
keywords: Argulus spp.; COI gene; DNA barcode; ornamental fishes; parasite eggs