title: Crystallization and preliminary crystallographic study of Feline infectious peritonitis virus main protease in complex with an inhibitor.
abstract: Feline infectious peritonitis virus (FIPV) causes a lethal systemic granulomatous disease in wild and domestic cats around the world. Currently, no effective vaccines or drugs have been developed against it. As a member of the genus Alphacoronavirus, FIPV encodes two polyprotein precursors required for genome replication and transcription. Each polyprotein undergoes extensive proteolytic processing, resulting in functional subunits. This process is mainly mediated by its genome-encoded main protease, which is an attractive target for antiviral drug design. In this study, the main protease of FIPV in complex with a Michael acceptor-type inhibitor was crystallized. The complex crystals diffracted to 2.5 Å resolution and belonged to space group I422, with unit-cell parameters a = 112.3, b = 112.3, c = 102.1 Å. There is one molecule per asymmetric unit.
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Tan
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06520, USA.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Fu
authors.affiliation: Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D016765:Coronavirus, Feline / Q000201:enzymology*; D003460:Crystallization; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry*; D011480:Protease Inhibitors / Q000737:chemistry*
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases
keywords: Feline infectious peritonitis virus; N3 inhibitor; main protease