title: Roadblock termination by reb1p restricts cryptic and readthrough transcription.
abstract: Widely transcribed compact genomes must cope with the major challenge of frequent overlapping or concurrent transcription events. Efficient and timely transcription termination is crucial to control pervasive transcription and prevent transcriptional interference. In yeast, transcription termination of RNA polymerase II (RNAPII) occurs via two possible pathways that both require recognition of termination signals on nascent RNA by specific factors. We describe here an additional mechanism of transcription termination for RNAPII and demonstrate its biological significance. We show that the transcriptional activator Reb1p bound to DNA is a roadblock for RNAPII, which pauses and is ubiquitinated, thus triggering termination. Reb1p-dependent termination generates a class of cryptic transcripts that are degraded in the nucleus by the exosome. We also observed transcriptional interference between neighboring genes in the absence of Reb1p. This work demonstrates the importance of roadblock termination for controlling pervasive transcription and preventing transcription through gene regulatory regions.
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France.
authors.forename: Jessie
authors.initials: J
authors.lastname: Colin
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France.
authors.forename: Tito
authors.initials: T
authors.lastname: Candelli
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France.
authors.forename: Odil
authors.initials: O
authors.lastname: Porrua
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France.
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Boulay
authors.affiliation: European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France.
authors.forename: François
authors.initials: F
authors.lastname: Lacroute
authors.affiliation: European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69117 Heidelberg, Germany; Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors.forename: Lars M
authors.initials: LM
authors.lastname: Steinmetz
authors.affiliation: Centre de Génétique Moléculaire, CNRS UPR3404, 91190 Gif sur Yvette, France. Electronic address: libri.domenico@ijm.univ-paris-diderot.fr.
authors.forename: Domenico
authors.initials: D
authors.lastname: Libri
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000378:metabolism*; D016681:Genome, Fungal; D008957:Models, Genetic; D012319:RNA Polymerase II / Q000378:metabolism*; D020871:RNA Stability; D012331:RNA, Fungal / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D054875:Ubiquitination
chemical_list: D004268:DNA-Binding Proteins; C065681:REB1 protein, S cerevisiae; D012331:RNA, Fungal; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D012319:RNA Polymerase II