title: Single nucleotide polymorphisms of mitochondrial DNA HVS-I and HVS-II in Chinese Bai ethnic group.
abstract: For forensic and population genetic purposes, a total of 125 unrelated volunteers' blood samples were collected from Chinese Bai ethnic minority group to analyze sequence variation of two hypervariable segments (HVS-I and HVS-II) in the mitochondrial DNA control region. Comparing the HVS-I and HVS-II sequences of the 125 Chinese Bais to the Anderson reference sequence, we found 86 polymorphic loci in HVS-I and 40 in HVS-II in mitochondrial DNA sequences of the Chinese Bai ethnic minority group, which defined 93 and 53 different haplotypes, respectively. Haplotype diversity and the mean pairwise differences were 0.992 ± 0.003 and 6.553 in HVS-I, and 0.877 ± 0.027 and 2.407 in HVS-II, respectively. We defined four macrohaplogroups R, M, N and D with the proportions ranging from 9.6% to 40.0%. With the analysis of the hypervariable domain from nucleotide 16 180-16 193 in HVS-I, our study revealed new haplotypes of sequence variations. In addition, the Fst metric, phylogenetic tree, and principal component analysis demonstrated a close genetic relationship between the Bai group and Chinese Han populations from South China, Changsha, and Guangdong. The results support that the Bai group is a multiorigin ethnic minority that has merged with the Chinese Han population.
authors.affiliation: Department of Forensic Medicine, Nanjing Medical University, Nanjing, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Cai-Yong
authors.initials: CY
authors.lastname: Yin
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Qian
authors.forename: Han-Ting
authors.initials: HT
authors.lastname: Fan
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Deng
authors.forename: Yu-Dang
authors.initials: YD
authors.lastname: Zhang
authors.forename: Hao-Tian
authors.initials: HT
authors.lastname: Meng
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shen
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Jin
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D005006:Ethnicity / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D025341:Principal Component Analysis
chemical_list: D004272:DNA, Mitochondrial
keywords: Chinese Bai ethnic group; HVS-I; HVS-II; Haplogroup assignment; mtDNA