title: Cooling water before panicle initiation increases chilling-induced male sterility and disables chilling-induced expression of genes encoding OsFKBP65 and heat shock proteins in rice spikelets.
abstract: In rice (Oryza sativa L.), chilling-induced male sterility increased when plants experienced low water temperature (Tw , 18 °C for 14 d) before panicle initiation. The number of mature pollen grains after chilling at the booting stage (12 °C for 5 d) was only 45% of total pollen grains in low-Tw plants, whereas it was 71% in normal-Tw plants (Tw not controlled; approximately 23 °C under air temperature of 26 °C/21 °C, day/night). Microarray and quantitative PCR analyses showed that many stress-responsive genes (including OsFKBP65 and genes encoding the large heat shock protein OsHSP90.1, heat-stress transcription factors and many small heat shock proteins) were strongly up-regulated by chilling in normal-Tw spikelets, but were unaffected or even down-regulated by chilling in low-Tw spikelets. OsAPX2 and genes encoding some other antioxidant enzymes were also significantly down-regulated by low Tw in chilled spikelets. The levels of lipid peroxidation products (malondialdehyde equivalents) were significantly increased in low-Tw spikelets by chilling. Ascorbate peroxidase activity in chilled spikelets was significantly lower in low-Tw plants than in normal-Tw plants. Our data suggest that an OsFKBP65-related chilling response, which protects proteins from oxidative damage, is indispensable for chilling tolerance but is lost in low-Tw spikelets.
authors.affiliation: Plant Physiology Group, NARO Tohoku Agricultural Research Center, Morioka, Iwate, 020-0198, Japan.
authors.forename: Kensaku
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Aoki
authors.affiliation: Plant Physiology Group, NARO Tohoku Agricultural Research Center, Morioka, Iwate, 020-0198, Japan.
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Matsumura
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Okamura
authors.affiliation: Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan.
authors.forename: Ryu
authors.initials: R
authors.lastname: Ohsugi
authors.affiliation: Crop Science Laboratory, Faculty of Agriculture, Iwate University, Morioka, Iwate, 020-8850, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Shimono
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D018384:Oxidative Stress; D010937:Plant Growth Regulators / Q000378:metabolism; D051479:Plant Infertility / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011058:Pollen / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014867:Water / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D014867:Water; D008315:Malondialdehyde; D000040:Abscisic Acid
keywords: abscisic acid (ABA); chilling-induced pollen sterility; ethylene; low water temperature; malondialdehyde (MDA); oxidative stress