title: A longitudinal proteomic assessment of peptide degradation and loss under acidic storage conditions.
abstract: Sample preparation prior to analysis by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) usually involves the storage of frozen peptide samples in an acidic environment for variable time periods. Questions arose in our laboratory regarding the stability of peptides in acid under medium- to long-term storage. Thus, a 10-month longitudinal study was designed to assess the effect on storage of tryptic peptides at -20 and -80°C under acidic conditions. Our conclusion and proposal from this evaluation is that the optimal storage conditions of peptide samples in acid for proteomic experiments is at -80°C and, ideally, as separate aliquots.
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Melanie
authors.initials: M
authors.lastname: Planyavsky
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Marie L
authors.initials: ML
authors.lastname: Huber
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: Nico A
authors.initials: NA
authors.lastname: Staller
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria.
authors.forename: André C
authors.initials: AC
authors.lastname: Müller
authors.affiliation: CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria. Electronic address: kbennett@cemm.oeaw.ac.at.
authors.forename: Keiryn L
authors.initials: KL
authors.lastname: Bennett
mesh_terms: D000595:Amino Acid Sequence; D053000:Analytic Sample Preparation Methods; D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000737:chemistry*; D059748:Proteolysis; D040901:Proteomics; D013997:Time Factors
chemical_list: D010455:Peptides
keywords: Acid degradation; Orbitrap Velos mass spectrometer; Peptide stability; Peptide storage; Proteomics; Storage temperature