title: Analytical quality assurance in veterinary drug residue analysis methods: matrix effects determination and monitoring for sulfonamides analysis.
abstract: In residue analysis of veterinary drugs in foodstuff, matrix effects are one of the most critical points. This work present a discuss considering approaches used to estimate, minimize and monitoring matrix effects in bioanalytical methods. Qualitative and quantitative methods for estimation of matrix effects such as post-column infusion, slopes ratios analysis, calibration curves (mathematical and statistical analysis) and control chart monitoring are discussed using real data. Matrix effects varying in a wide range depending of the analyte and the sample preparation method: pressurized liquid extraction for liver samples show matrix effects from 15.5 to 59.2% while a ultrasound-assisted extraction provide values from 21.7 to 64.3%. The matrix influence was also evaluated: for sulfamethazine analysis, losses of signal were varying from -37 to -96% for fish and eggs, respectively. Advantages and drawbacks are also discussed considering a workflow for matrix effects assessment proposed and applied to real data from sulfonamides residues analysis.
authors.affiliation: Instituto de Química, UFRGS, Porto Alegre, RS, Brazil; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário-LANAGRO/RS, Porto Alegre, RS, Brazil. Electronic address: rodrigo.hoff@agricultura.gov.br.
authors.forename: Rodrigo Barcellos
authors.initials: RB
authors.lastname: Hoff
authors.affiliation: Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário-LANAGRO/RS, Porto Alegre, RS, Brazil.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rübensam
authors.affiliation: Instituto de Química, UFRGS, Porto Alegre, RS, Brazil; Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário-LANAGRO/RS, Porto Alegre, RS, Brazil.
authors.forename: Louise
authors.initials: L
authors.lastname: Jank
authors.affiliation: Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Nacional Agropecuário-LANAGRO/RS, Porto Alegre, RS, Brazil; Faculdade de Farmácia, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Fabiano
authors.initials: F
authors.lastname: Barreto
authors.affiliation: Instituto de Química, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Maria do Carmo Ruaro
authors.initials: Mdo C
authors.lastname: Peralba
authors.affiliation: Instituto de Química, UFRGS, Porto Alegre, RS, Brazil.
authors.forename: Tânia Mara
authors.initials: TM
authors.lastname: Pizzolato
authors.affiliation: Department of Environmental Chemistry, IDAEA, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Silvia Díaz-Cruz
authors.affiliation: Department of Environmental Chemistry, IDAEA, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain; Catalan Institute for Water Research (ICRA), Parc Científic i Tecnològic de la Universitat de Girona, C/Emili Grahit, 101 Edifici H2O, E-17003 Girona, Spain.
authors.forename: Damià
authors.initials: D
authors.lastname: Barceló
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002417:Cattle; D002645:Chickens; D002853:Chromatography, Liquid / Q000592:standards; D004350:Drug Residues / Q000302:isolation & purification*; D004531:Eggs / Q000032:analysis*; D005399:Fishes; D008099:Liver / Q000737:chemistry*; D009928:Organ Specificity; D011786:Quality Control; D013449:Sulfonamides / Q000302:isolation & purification*; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000592:standards; D019155:Veterinary Drugs / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D013449:Sulfonamides; D019155:Veterinary Drugs
keywords: Antibiotics; Control charts; Ion suppression; Matrix effects; Quality control; Sulfonamides; Validation; Veterinary drug residues