title: C4d immunoreactivity of intraoperative zero-hour biopsy in renal allograft.
abstract: C4d deposition in the peritubular capillaries is known to be correlated with antibody-mediated rejection (AMR) in renal allografts. An intraoperative zero-hour biopsy during transplantation is considered an indicator to indirectly determine the status of the donor kidney. In this study, we investigated the relationship between C4d immunoreactivity of intraoperative zero-hour biopsy in renal allograft, thought to be due to donor condition, and acute rejection episodes during follow-up. We collected 147 renal transplantation cases examining intraoperative zero-hour biopsy with C4d immunohistochemical staining. All cases were from the Seoul National University Hospital between 2010 and 2011. Of the 147 cases, 24 (16.3%) showed strong C4d staining in the glomeruli, 38 (25.9%) showed weak staining, and the remainder (57.8%) showed negative staining. Nine cases (6.1%) showed positive C4d staining in the arterioles, and the remainder (93.9%) were negative. There were no significant differences between acute T-cell-mediated rejection and acute AMR episodes in the renal allograft specimens during follow-up according to the glomerular or arteriolar C4d immunoreactivity of the intraoperative zero-hour biopsy specimens.
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Suh
authors.affiliation: Department of Pathology, Good Moonhwa Hospital, Busan, Korea.
authors.forename: H W
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Pathology, Seoul National University College of Medicine, Seoul, Korea. Electronic address: blue7270@snu.ac.kr.
authors.forename: K C
authors.initials: KC
authors.lastname: Moon
mesh_terms: D000917:Antibody Formation; D001706:Biopsy; D002196:Capillaries / Q000378:metabolism; D015935:Complement C4b / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D007432:Intraoperative Period; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000473:pathology; D007678:Kidney Glomerulus; D016030:Kidney Transplantation; D008875:Middle Aged; D010446:Peptide Fragments / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
chemical_list: D010446:Peptide Fragments; D015935:Complement C4b; C032261:complement C4d