title: Effect of sequential medium with fibroblast growth factor-10 and follicle stimulating hormone on in vitro development of goat preantral follicles.
abstract: A sequential medium with fibroblast growth factor-10 (FGF-10) and follicle stimulating hormone (FSH) was evaluated on the survival, ultrastructure, activation and growth rate of caprine preantral follicles submitted to long-term culture, aiming to establish an ideal in vitro culture system. Ovarian fragments were cultured for 16 days in α-MEM(+) alone or supplemented with FGF-10 and/or FSH added sequentially on different days of culture. Ovarian fragments were cultured during the first (days 0-8) and second (days 8-16) halves of the culture period, generating 10 treatments: α-MEM(+)/α-MEM(+) (cultured control), FSH/FSH, FSH/FGF-10, FSH/FSH+FGF-10, FGF-10/FGF-10, FGF-10/FSH, FGF-10/FSH+FGF-10, FSH+FGF-10/FSH+FGF-10, FSH+FGF-10/FSH and FSH+FGF-10/FGF-10. Follicle morphology, viability and ultrastructure were analyzed. The FSH/FGF-10 treatment showed a higher (P<0.05) percentage of normal follicles compared to all other treatments. In addition, follicles from the FSH/FGF-10 treatment maintained ultrastructural integrity after the culture period. After 16 days of culture, the FSH/FGF-10 and FSH/FSH treatments showed a higher percentage of activation compared to the cultured control (α-MEM(+)/α-MEM(+)). Moreover, the FSH/FGF-10 treatment promoted greater follicular and oocyte diameters compared to the fresh control. In conclusion, this study showed that a sequential medium with FSH followed by FGF-10 (FSH/FGF-10 and FSH/FSH) maintains follicular viability and ultrastructure and promotes transition from the primordial to primary stage (activation) and growth in goat preantral follicles cultured in vitro.
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Almeida
authors.affiliation: Postgraduate Biotechnology, Potiguar University Laureate International Universities, Av. Senador Salgado Filho, 1610, Lagoa Nova, Natal, 59056-000, CE, Brazil. Electronic address: dmmvet@hotmail.com.
authors.forename: D M
authors.initials: DM
authors.lastname: Magalhães-Padilha
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Araújo
authors.affiliation: Laboratory of Animal Reproduction, Veterinary Department, Federal University of Viçosa, Viçosa, 36570-000, MG, Brazil.
authors.forename: S L
authors.initials: SL
authors.lastname: Costa
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: R N
authors.initials: RN
authors.lastname: Chaves
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: C A P
authors.initials: CA
authors.lastname: Lopes
authors.affiliation: Laboratory of Ultrastructural, CPqAM/Fiocruz, Federal University of Pernanbuco, Recife, PE, Brazil.
authors.forename: M A M
authors.initials: MA
authors.lastname: Donato
authors.affiliation: Laboratory of Ultrastructural, CPqAM/Fiocruz, Federal University of Pernanbuco, Recife, PE, Brazil.
authors.forename: C A
authors.initials: CA
authors.lastname: Peixoto
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Campello
authors.affiliation: Department of Physiology, Institute of Biosciences, State University Paulista, Botucatu, 18618-000, SP, Brazil.
authors.forename: J Buratini
authors.initials: JB
authors.lastname: Junior
authors.affiliation: Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Av. Paranjana 1700, Campus Itaperi, Fortaleza, 60740-000, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D003470:Culture Media / Q000737:chemistry; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006041:Goats; D006080:Ovarian Follicle / Q000187:drug effects* / Q000254:growth & development*; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D003470:Culture Media; D051526:Fibroblast Growth Factor 10; D005640:Follicle Stimulating Hormone
keywords: Caprine; FGF-10; FSH; Folliculogenesis; Preantral follicle