title: Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca).
abstract: The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33) did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin, and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH, and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10's activation of biosynthetic steps is inhibited by the strawberry repressor MYB1.
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited Auckland, New Zealand.
authors.forename: Kui
authors.initials: K
authors.lastname: Lin-Wang
authors.affiliation: Plant and Food Research Limited Palmerston North, New Zealand.
authors.forename: Tony K
authors.initials: TK
authors.lastname: McGhie
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited Auckland, New Zealand.
authors.forename: Mindy
authors.initials: M
authors.lastname: Wang
authors.affiliation: Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Agricultural University Lanzhou, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited Auckland, New Zealand.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Warren
authors.affiliation: Plant and Food Research Limited Te Puke, New Zealand.
authors.forename: Roy
authors.initials: R
authors.lastname: Storey
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited Auckland, New Zealand.
authors.forename: Richard V
authors.initials: RV
authors.lastname: Espley
authors.affiliation: The New Zealand Institute for Plant and Food Research Limited Auckland, New Zealand ; School of Biological Sciences, University of Auckland Auckland, New Zealand.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Allan
keywords: Fragaria vesca; FvMYB10; anthocyanin; regulation; strawberry; transcription factor