title: Proteomics investigation reveals cell death-associated proteins of basidiomycete fungus Trametes versicolor treated with Ferruginol.
abstract: Ferruginol has antifungal activity against wood-rot fungi (basidiomycetes). However, specific research on the antifungal mechanisms of ferruginol is scarce. Two-dimensional gel electrophoresis and fluorescent image analysis were employed to evaluate the differential protein expression of wood-rot fungus Trametes versicolor treated with or without ferruginol. Results from protein identification of tryptic peptides via liquid chromatography–electrospray ionization tandem mass spectrometry (LC–ESI-MS/MS) analyses revealed 17 protein assignments with differential expression. Downregulation of cytoskeleton β-tubulin 3 indicates that ferruginol has potential to be used as a microtubule-disrupting agent. Downregulation of major facilitator superfamily (MFS)–multiple drug resistance (MDR) transporter and peroxiredoxin TSA1 were observed, suggesting reduction in self-defensive capabilities of T. versicolor. In addition, the proteins involved in polypeptide sorting and DNA repair were also downregulated, while heat shock proteins and autophagy-related protein 7 were upregulated. These observations reveal that such cellular dysfunction and damage caused by ferruginol lead to growth inhibition and autophagic cell death of fungi.
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Chen
authors.forename: Ting-Feng
authors.initials: TF
authors.lastname: Yeh
authors.forename: Fang-Hua
authors.initials: FH
authors.lastname: Chu
authors.forename: Fu-Lan
authors.initials: FL
authors.lastname: Hsu
authors.forename: Shang-Tzen
authors.initials: ST
authors.lastname: Chang
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D016923:Cell Death / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics; D005659:Fungicides, Industrial / Q000494:pharmacology*; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D040901:Proteomics; D055454:Trametes / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D050257:Tubulin Modulators
chemical_list: D045784:Abietanes; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D050257:Tubulin Modulators; C431074:ferruginol