title: Characterization of the biological activities and physicochemical characteristics of recombinant bovine interferon-α₁₄.
abstract: A BoIFN-α gene that included signal sequence was amplified from bovine liver genomic DNA. The gene was named BoIFN-α14 according to the position at which the encoded gene of the bovine IFN was located in the bovine genome. The sequence included a 23-amino acid signal peptide and 166-amino acid mature peptide. The structural characteristics and phylogenetic relationships of the BoIFN-α14 gene were analyzed. A recombinant mature BoIFN-α14 (rBoIFN-α14) was expressed in the yeast Pichia pastoris. Antiviral activity, antiproliferation activity and physicochemical characteristics were determined in vitro. Recombinant BoIFN-α14 exhibited a considerable antiviral activity against Vesicular stomatitis virus (VSV), which was neutralized by a rabbit polyclonal anti-rBoIFN-α antibody, and could inhibit cell proliferation. It was also found to be highly sensitive to trypsin and stable at pH 2.0 or 65°C. This study revealed that rBoIFN-α14 has the typical characteristics of IFN-α and can be used for both research and industrial application.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Shao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; Synergetic Innovation Center of Food Safety and Nutrition, Harbin 150030, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Synergetic Innovation Center of Food Safety and Nutrition, Harbin 150030, China. Electronic address: gaomingchun@163.com.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Synergetic Innovation Center of Food Safety and Nutrition, Harbin 150030, China. Electronic address: jwwang@neau.edu.cn.
authors.forename: Junwei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D001483:Base Sequence; D015153:Blotting, Western; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors / Q000378:metabolism; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D016898:Interferon-alpha / Q000737:chemistry / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology*; D013696:Temperature; D014170:Transformation, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D011994:Recombinant Proteins
keywords: Antiproliferation activity; Antiviral activity; Expression; Physicochemical characteristics; Recombinant BoIFN-α(14)