title: Generation and structural validation of a library of diverse xyloglucan-derived oligosaccharides, including an update on xyloglucan nomenclature.
abstract: Xyloglucans are structurally complex plant cell wall polysaccharides that are involved in cell growth and expansion, energy metabolism, and signaling. Determining the structure-function relationships of xyloglucans would benefit from the availability of a comprehensive and structurally diverse collection of rigorously characterized xyloglucan oligosaccharides. Here, we present a workflow for the semi-preparative scale generation and purification of neutral and acidic xyloglucan oligosaccharides using a combination of enzymatic and chemical treatments and size-exclusion chromatography. Twenty-six of these oligosaccharides were purified to near homogeneity and their structures validated using a combination of matrix-assisted laser desorption/ionization mass spectrometry, high-performance anion exchange chromatography, and 1H nuclear magnetic resonance spectroscopy. Mass spectrometry and analytical chromatography were compared as methods for xyloglucan oligosaccharide quantification. 1H chemical shifts were assigned using two-dimensional correlation spectroscopy. A comprehensive update of the nomenclature describing xyloglucan side-chain structures is provided for reference.
authors.affiliation: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd, Athens, GA 30602, USA.
authors.forename: Sami T
authors.initials: ST
authors.lastname: Tuomivaara
authors.affiliation: Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan.
authors.forename: Katsuro
authors.initials: K
authors.lastname: Yaoi
authors.affiliation: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd, Athens, GA 30602, USA.
authors.forename: Malcolm A
authors.initials: MA
authors.lastname: O'Neill
authors.affiliation: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd, Athens, GA 30602, USA; Department of Biochemistry and Molecular Biology, The University of Georgia, Athens, GA 30602, USA. Electronic address: will@ccrc.uga.edu.
authors.forename: William S
authors.initials: WS
authors.lastname: York
mesh_terms: D031002:Acer / Q000737:chemistry; D002240:Carbohydrate Sequence; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D003912:Dextrins / Q000737:chemistry; D005936:Glucans / Q000737:chemistry* / Q000302:isolation & purification; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010084:Oxidation-Reduction; D012639:Seeds / Q000737:chemistry; D032503:Selaginellaceae / Q000737:chemistry; D013402:Sugar Alcohols / Q000737:chemistry; D009626:Terminology as Topic; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification; D014995:Xylosidases / Q000378:metabolism
chemical_list: D003912:Dextrins; D005936:Glucans; D009844:Oligosaccharides; D013402:Sugar Alcohols; D014990:Xylans; C029353:xyloglucan; D002482:Cellulose; C068994:cellodextrin; D014995:Xylosidases
keywords: Glycosyl hydrolase; Nuclear magnetic resonance spectroscopy; Oligosaccharide; Plant cell wall; Size-exclusion chromatography; Xyloglucan