title: AEG-1 3'-untranslated region functions as a ceRNA in inducing epithelial-mesenchymal transition of human non-small cell lung cancer by regulating miR-30a activity.
abstract: Competitive endogenous messenger RNA regulates the transcription of other RNA moleculars through competing for the shared microRNAs. This study was carried out to explore the regulation of AEG-1 messenger RNA as a competitive endogenous RNA in the epithelial-mesenchymal transition and metastasis of lung tumor cells. It is shown that the epithelial-mesenchymal transition was associated with the down-regulation of miR-30a, up-regulation of AEG-1 and mesenchymal markers (Snail and Vimentin); miR-30a inhibited the metastasis of lung tumor A549 cells in vitro, whereas AEG-1 promoted it. These results suggested the potential linkage between miR-30a and genes (AEG-1, Snail and Vimentin) in the epithelial-mesenchymal transition and metastasis of lung tumor cell. It was verified later that the 3'-untranslated regions of AEG-1, Snail and Vimentin bind to miR-30a in A549 cells. Therefore, a competitive endogenous RNAs regulatory network among AEG-1, Snail and Vimentin mediated via competitive binding to miR-30a was proved. That is, the 3'-untranslated region of AEG-1, functioning as the competitive endogenous RNAs, indirectly regulated the expression of Vimentin and Snail in inducing epithelial-mesenchymal transition of human non-small cell lung cancer. In conclusion, our findings demonstrated a competitive endogenous RNAs regulatory network which will help understand the metastasis mechanisms of lung cancer and improve the prevention and treatment of lung cancer.
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009 Nanjing, China; Ningxia Key Laboratory of Cerebrocranial Diseases, School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Kunmei
authors.initials: K
authors.lastname: Liu
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China. Electronic address: guoletian1982@163.com.
authors.forename: Le
authors.initials: L
authors.lastname: Guo
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009 Nanjing, China.
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Guo
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009 Nanjing, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009 Nanjing, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: Ningxia Key Laboratory of Cerebrocranial Diseases, School of Laboratory Medicine, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: Medical School of Nantong University, Nantong University, Nantong 226001, China.
authors.forename: Runting
authors.initials: R
authors.lastname: Yin
authors.affiliation: School of Life Science and Technology, State Key Laboratory of Natural Medicines, China Pharmaceutical University, 210009 Nanjing, China. Electronic address: lkm198507@126.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Xi
mesh_terms: D020413:3' Untranslated Regions; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008565:Membrane Proteins; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D015815:Cell Adhesion Molecules; C546086:MIRN30b microRNA, human; C483039:MTDH protein, human; D008565:Membrane Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D014157:Transcription Factors
keywords: AEG-1; Competitive endogenous RNAs (ceRNAs); Epithelial–mesenchymal transition (EMT); Lung cancer; Snail; Vimentin; miR-30a