title: Using a non-invasive assessment of lung injury in a murine model of acute lung injury.
abstract: Arterial oxygen saturation has not been assessed sequentially in conscious mice as a direct consequence of an in vivo murine model of acute lung injury. Here, we report daily changes in arterial oxygen saturation and other cardiopulmonary parameters by using infrared pulse oximetry following intratracheal lipopolysaccharide (IT-LPS) for up to 9 days, and following IT-phosphate buffered saline up to 72 h as a control. We show that arterial oxygen saturation decreases, with maximal decline at 96 h post IT-LPS. Blood oxygen levels negatively correlate with 7 of 10 quantitative markers of murine lung injury, including neutrophilia and interleukin-6 expression. This identifies infrared pulse oximetry as a method to non-invasively monitor arterial oxygen saturation following direct LPS instillations.
authors.affiliation: Department of Clinical Respiratory Sciences , Centre for Translational Inflammation Research, University of Birmingham Research Laboratories, Queen Elizabeth Hospital , Birmingham , UK.
authors.forename: Siân
authors.initials: S
authors.lastname: Lax
authors.affiliation: Department of Anaesthetics, Pain Medicine and Intensive Care, Faculty of Medicine , Imperial College London, Chelsea and Westminster Hospital , London , UK.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Wilson
authors.affiliation: Department of Anaesthetics, Pain Medicine and Intensive Care, Faculty of Medicine , Imperial College London, Chelsea and Westminster Hospital , London , UK.
authors.forename: Masao
authors.initials: M
authors.lastname: Takata
authors.affiliation: Department of Clinical Respiratory Sciences , Centre for Translational Inflammation Research, University of Birmingham Research Laboratories, Queen Elizabeth Hospital , Birmingham , UK.
authors.forename: David R
authors.initials: DR
authors.lastname: Thickett
keywords: Neutrophil Biology; Pulmonary oedema; Respiratory Infection