title: Changes in chemical components and cytotoxicity at different maturity stages of Pleurotus eryngii fruiting body.
abstract: The present study investigated the changes of the chemical components and cytotoxicity potency at 5 developmental stages of Pleurotus eryngii fruiting body. The carbohydrate and protein contents increased along the maturity of fruiting body while fat content decreased. By comparison, the polysaccharide-protein fractions had the highest antiproliferative effect on SGC-7901 and HepG-2 cells in vitro and increasing activity with growing maturity of P. eryngii fruiting body.The maturation process increased the protein content and acid property through the enhanced relative abundance of Asp, Thr, and Glu in polysaccharide-protein fractions. Further purification and electrophoresis identified that the polysaccharide-protein PEG-1with three subunits possibly was the target cytotoxical component. Our findings proved that mature fruiting body of P. eryngii containing these polysaccharide-proteins possessed highly nutritional values and therapeutical benefits.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University , Zhenjiang 212013, P. R. China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Cui
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Wenjing
authors.initials: W
authors.lastname: Sun
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.forename: Lifeng
authors.initials: L
authors.lastname: Ping
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry / Q000254:growth & development*; D005656:Fungal Proteins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020076:Pleurotus / Q000737:chemistry* / Q000254:growth & development; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides
keywords: Pleurotus eryngii; chemical component; cytotoxical activity; maturity stages