title: Comparative experimental subcutaneous glanders and melioidosis in the common marmoset (Callithrix jacchus).
abstract: Glanders and melioidosis are caused by two distinct Burkholderia species and have generally been considered to have similar disease progression. While both of these pathogens are HHS/CDC Tier 1 agents, natural infection with both these pathogens is primarily through skin inoculation. The common marmoset (Callithrix jacchus) was used to compare disease following experimental subcutaneous challenge. Acute, lethal disease was observed in marmosets following challenge with between 26 and 1.2 × 10(8) cfu Burkholderia pseudomallei within 22-85 h. The reproducibility and progression of the disease were assessed following a challenge of 1 × 10(2) cfu of B. pseudomallei. Melioidosis was characterised by high levels of bacteraemia, focal microgranuloma progressing to non-necrotic multifocal solid lesions in the livers and spleens and multi-organ failure. Lethal disease was observed in 93% of animals challenged with Burkholderia mallei, occurring between 5 and 10.6 days. Following challenge with 1 × 10(2) cfu of B. mallei, glanders was characterised with lymphatic spread of the bacteria and non-necrotic, multifocal solid lesions progressing to a multifocal lesion with severe necrosis and pneumonia. The experimental results confirmed that the disease pathology and presentation is strikingly different between the two pathogens. The marmoset provides a model of the human syndrome for both diseases facilitating the development of medical countermeasures.
authors.affiliation: Biomedical Sciences, Defence Science and Technology Laboratory (Dstl), Salisbury, Wiltshire, UK.
authors.forename: Michelle
authors.initials: M
authors.lastname: Nelson
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: Rachel E
authors.initials: RE
authors.lastname: Dean
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Ngugi
authors.forename: Sophie J
authors.initials: SJ
authors.lastname: Smither
authors.forename: Timothy P
authors.initials: TP
authors.lastname: Atkins
authors.forename: Mark S
authors.initials: MS
authors.lastname: Lever
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D058491:Bacterial Load; D042726:Burkholderia mallei; D016957:Burkholderia pseudomallei; D002144:Callithrix; D004195:Disease Models, Animal; D005260:Female; D005896:Glanders / Q000382:microbiology* / Q000401:mortality / Q000473:pathology*; D007279:Injections, Subcutaneous; D008297:Male; D008554:Melioidosis / Q000382:microbiology* / Q000401:mortality / Q000473:pathology*; D012720:Severity of Illness Index
chemical_list: D000942:Antigens, Bacterial
keywords: Burkholderia; animal model; histology