title: Peste des petits ruminants virus-like particles induce both complete virus-specific antibodies and virus neutralizing antibodies in mice.
abstract: Peste des petits ruminants virus (PPRV), an etiological agent of peste des petits ruminants (PPR), is classified into the genus Morbillivirus in the family Paramyxoviridae. In a previous study, a recombinant baculovirus has been constructed to co-express the PPRV matrix (M), haemagglutinin (H) and nucleocapsid (N) proteins in insect cells, causing budding of PPR virus-like particles (VLPs) from insect cell membranes by viewing of ultrathin section with a transmission electron microscope. In this follow-up study, these PPR VLPs were purified by sucrose density gradient centrifugation for immunizing mice twice. Three weeks post-primary immunization and 2 weeks post-secondary immunization, all serum samples were obtained and subsequently subjected to indirect ELISA detection on complete virus-specific antibodies. In addition, all serum samples, which were collected 2 weeks post-secondary immunization, were used for virus neutralization test on PPRV neutralizing antibodies. The results showed that the purified PPR VLPs induced both types of antibodies mentioned above in mice, indicating a given potential of VLP-based vaccine candidate against PPR.
authors.affiliation: OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
authors.forename: Fuxiao
authors.initials: F
authors.lastname: Liu
authors.affiliation: OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wu
authors.affiliation: OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zou
authors.affiliation: OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: OIE Reference Laboratory for Peste des Petits Ruminants, National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, No.369 Nanjing Road, Qingdao, Shandong 266032, China. Electronic address: wangzhiliang@cahec.cn.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000276:immunology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000276:immunology*; D014611:Vaccination / Q000379:methods; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D014763:Viral Matrix Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D006388:Hemagglutinins; D019590:Nucleocapsid Proteins; D058425:Vaccines, Virus-Like Particle; D014763:Viral Matrix Proteins
keywords: ELISA; Neutralizing antibody; Peste des petits ruminants virus; Virus neutralization test; Virus-like particle