title: A one-step electrochemiluminescence immunosensor preparation for ultrasensitive detection of carbohydrate antigen 19-9 based on multi-functionalized graphene oxide.
abstract: A one-step electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of carbohydrate antigen 19-9 (CA19-9) was developed based on multi-functionalized graphene oxide (GO), which was prepared with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and CA19-9 antibody (anti-CA19-9) chemically bound to the surface of magnetic GO (nanoFe3O4@GO). ABEI and anti-CA19-9 acted as the electrochemiluminophore and the capture device for CA19-9 respectively. NanoFe3O4@GO enabled all the ABEI immobilized molecules electrochemically active due to its good conductivity, and brought multi-functionalized GO attracted on the surface of magnetic glass carbon electrode through magnetism. Thus the ECL immunosensor could be prepared through a one-step process that facilitates ultrasensitive detection of CA19-9. Under optimal conditions, the ECL intensity of the immunosensor decreased proportionally to the logarithmic concentrations of CA19-9 in the range of 0.001-5U/mL with a detection limit of 0.0005U/mL. This one-step ECL immunosensor showed good performance in specificity, stability, reproducibility, regeneration and application. It opened a new avenue to apply multi-functionalized bionanomaterials in ECL immunoassay.
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Sha
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China. Electronic address: nbuguo@163.com.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Chen
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
authors.forename: Sui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Faculty of Materials Science and Chemical Engineering, The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Ningbo University, Ningbo 315211, PR China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Ge
authors.affiliation: Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.affiliation: School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, PR China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D018395:CA-19-9 Antigen / Q000097:blood*; D055664:Electrochemical Techniques; D006108:Graphite; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000031:analogs & derivatives; D058185:Magnetite Nanoparticles; D015203:Reproducibility of Results
chemical_list: D018395:CA-19-9 Antigen; D058185:Magnetite Nanoparticles; C561085:N-(4-aminobutyl)-N-ethylisoluminol; D008165:Luminol; D006108:Graphite
keywords: Carbohydrate antigen 19-9; Electrochemiluminescence immunosensor; Multi-functionalized graphene oxide; N-(4-aminobutyl)-N-ethylisoluminol; One-step preparation