title: Immune and anti-oxidant effects of in ovo selenium proteinate on post-hatch experimental avian necrotic enteritis.
abstract: This study was conducted to investigate the effects of in ovo administration of selenium (Se) incorporated into hydrolyzed soybean protein (B-Taxim [BT]) on protection against experimental avian necrotic enteritis (NE). Broiler eggs were injected with either 100 μl of PBS alone (BT0), or 20 or 40 μg/egg of BT in PBS (BT20, BT40) at 18 days of embryogenesis. On day 14 post-hatch, the chickens were uninfected or orally infected with 1.0 × 10(4) oocysts of Eimeria maxima (E. maxima). On day 18 post-hatch, E. maxima-infected chickens were orally infected with 1.0 × 10(9) CFU of Clostridium perfringens (C. perfringens). Compared with untreated and infected BT0 controls, BT20 and/or BT40 birds showed increased body weights, decreased fecal shedding of E. maxima oocysts, lower serum α-toxin and NetB levels, increased levels of serum antibodies against C. perfringens α-toxin and NetB toxin, decreased levels of serum malondialdehyde, reduced serum catalase and superoxide dismutase catalytic activities, and increased intestinal levels of gene transcripts encoding interleukin (IL)-1β, IL-6, IL-8, and peroxiredoxin-6, but decreased levels of transcripts for catalase and glutathione peroxidase. Interestingly, transcript levels for inducible nitric oxide synthase and paraoxonase/arylesterase 2 were decreased in the BT20 group and increased in the BT40 group, compared with BT0 controls. These results indicate that in ovo administration of broiler chickens with a Se-containing protein hydrolysate enhanced protection against experimental NE possibly by altering the expression of proinflammatory and anti-oxidant genes and their downstream pathways.
authors.forename: Sung Hyen
authors.initials: SH
authors.lastname: Lee
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Seung I
authors.initials: SI
authors.lastname: Jang
authors.forename: Misun
authors.initials: M
authors.lastname: Jeong
authors.forename: Duk Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Shouzhen
authors.initials: S
authors.lastname: Xu
authors.forename: Seung Kyu
authors.initials: SK
authors.lastname: Lee
authors.forename: Jung Bong
authors.initials: JB
authors.lastname: Kim
authors.forename: Hong Ju
authors.initials: HJ
authors.lastname: Park
authors.forename: Haeng Ran
authors.initials: HR
authors.lastname: Kim
authors.forename: David M
authors.initials: DM
authors.lastname: Bravo
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002645:Chickens / Q000276:immunology* / Q000382:microbiology; D003015:Clostridium Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D003016:Clostridium perfringens / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D004751:Enteritis / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D009336:Necrosis / Q000662:veterinary; D010063:Ovum / Q000378:metabolism; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D012643:Selenium / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016207:Cytokines; D012643:Selenium