title: Antibiotic Susceptibility Pattern of ESβL Producing Klebsiella pneumoniae Isolated from Urine Samples of Pregnant Women in Karnataka.
abstract: BACKGROUND
  Klebsiella pneumoniae possess a new problem to health care professionals worldwide, which complicates and limits therapeutic options. It is one of the leading nosocomial bacterial pathogens, and the present study aims to determine the prevalence of ESβL producing K. pneumoniae isolates with their antibiotic susceptibility pattern in urine samples of the pregnant women with UTI.
  
  
  METHODS
  Using standard isolation and identification procedures a total of 41 isolates were obtained from 417 midstream urine samples of pregnant women with suspected UTI in Karnataka. The antibiotic resistance profile of each isolate was performed by Kirby-Bauer disc diffusion method and ESβL production by standard phenotypic method.
  
  
  RESULTS
  Isolation rate of K. pneumoniae in pregnant women was 19.9% and overall incidence rate was 9.8%. Among the 41 K. pneumoniae isolates, 26 (63.4%) were ESβL producers and all were found to be Multi Drug Resistance (MDR). The antibiotic susceptibility test (AST) for the isolates revealed that the highest number of K. pneumoniae were resistant to ampicillin (75.6%) followed by, nitrofurontoin and cefuroxime (73.1%) and least to chloramphenicol (12.1%). ESβL producers were highly resistance to nitrofurontoin (69.2%) and cotrimonazole (65.2%) and lower resistance was (7.6%) to amaikacin, observed. A higher resistance pattern to these two antibiotics was observed against ESβL non producing K. pneumonia but lowest to polymyxin B (13.3%) instead of amikacin (26.6%). All the isolates were found to be susceptible to imipenem.
  
  
  CONCLUSIONS
  Present investigation revealed high prevalence of MDR- ESβL producing Klebsiella pneumoniae, which indicates dire need for effective ESβL surveillance in the community by using cost effective antimicrobials agents.
authors.affiliation: Research Scholar, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University , Gulbarga, India .
authors.forename: Manjula
authors.initials: M
authors.lastname: N G
authors.affiliation: Research Scholar, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University , Gulbarga, India .
authors.forename: Girish
authors.initials: G
authors.lastname: C Math
authors.affiliation: Guest Faculty, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University , Gulbarga, India .
authors.forename: Kavita
authors.initials: K
authors.lastname: Nagshetty
authors.affiliation: Additional Professor, Department of Neuromicrobiology, National Institute of Mental Health and Neurosciences , Bangalore, India .
authors.forename: Shripad A
authors.initials: SA
authors.lastname: Patil
authors.affiliation: Professor, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University , Gulbarga, India .
authors.forename: Subhashchandra M
authors.initials: SM
authors.lastname: Gaddad
authors.affiliation: Professor, Department of Post Graduate Studies and Research in Microbiology, Gulbarga University , Gulbarga, India .
authors.forename: Channappa T
authors.initials: CT
authors.lastname: Shivannavar
keywords: ESβL; Enterobacteriaceae K. pneumoniae; UTI. Pregnant women; Uropathogens