title: Omega-3 fatty acid production from enzyme saccharified hemp hydrolysate using a novel marine thraustochytrid strain.
abstract: In this work, a newly isolated marine thraustochytrid strain, Schizochytrium sp. DT3, was used for omega-3 fatty acid production by growing on lignocellulose biomass obtained from local hemp hurd (Cannabis sativa) biomass. Prior to enzymatic hydrolysis, hemp was pretreated with sodium hydroxide to open the biomass structure for the production of sugar hydrolysate. The thraustochytrid strain was able to grow on the sugar hydrolysate and accumulated polyunsaturated fatty acids (PUFAs). At the lowest carbon concentration of 2%, the PUFAs productivity was 71% in glucose and 59% in the sugars hydrolysate, as a percentage of total fatty acids. Saturated fatty acids (SFAs) levels were highest at about 49% of TFA using 6% glucose as the carbon source. SFAs of 41% were produced using 2% of SH. This study demonstrates that SH produced from lignocellulose biomass is a potentially useful carbon source for the production of omega-3 fatty acids in thraustochytrids, as demonstrated using the new strain, Schizochytrium sp. DT3.
authors.affiliation: Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Geelong, Waurn Ponds, Victoria 3217, Australia.
authors.forename: Adarsha
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Geelong, Waurn Ponds, Victoria 3217, Australia.
authors.forename: Reinu E
authors.initials: RE
authors.lastname: Abraham
authors.affiliation: Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Geelong, Waurn Ponds, Victoria 3217, Australia.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Barrow
authors.affiliation: Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Geelong, Waurn Ponds, Victoria 3217, Australia. Electronic address: munish.puri@deakin.edu.au.
authors.forename: Munish
authors.initials: M
authors.lastname: Puri
mesh_terms: D059001:Aquatic Organisms / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D018533:Biomass; D002188:Cannabis / Q000737:chemistry*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002241:Carbohydrates / Q000032:analysis; D002475:Cellobiose / Q000494:pharmacology; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006868:Hydrolysis; D010802:Phylogeny; D058009:Stramenopiles / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000494:pharmacology
chemical_list: D002241:Carbohydrates; D004952:Esters; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D002475:Cellobiose; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Bioactives; Hemp; Pretreatment; Schizochytrium; Sugar hydrolysate