title: Crystallization and preliminary X-ray crystallographic study of human Hikeshi, a new nuclear transport receptor for Hsp70.
abstract: Hikeshi is a new nuclear transport receptor that plays an important role in the nuclear import of Hsp70 heat-shock proteins under thermal stress. Wild-type human Hikeshi and its Phe97Ala mutant were overproduced and purified using an Escherichia coli expression system. The purified proteins were crystallized using the hanging-drop vapour-diffusion technique. Wild-type crystals grew in space group C2221, with unit-cell parameters a = 61.1, b = 137.8, c = 97.9 Å, α = 90.0, β = 90.0, γ = 90.0°. Phe97Ala mutant crystals were obtained in space group P32, with unit-cell parameters a = 85.7, b = 85.7, c = 69.1 Å, α = 90.0, β = 90.0, γ = 120.0°. These crystals diffracted to 1.8 and 2.5 Å resolution, respectively. This study is the first to yield structural insight into this highly unusual fourth import receptor after importins, NTF2 and TAP.
authors.affiliation: College of Pharmacy, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Jinsue
authors.initials: J
authors.lastname: Song
authors.affiliation: College of Pharmacy, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, Republic of Korea.
authors.forename: Soo Jae
authors.initials: SJ
authors.lastname: Lee
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism*; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D021381:Protein Transport
chemical_list: D002352:Carrier Proteins; D018840:HSP70 Heat-Shock Proteins; C572913:hikeshi protein, human
keywords: FG-nucleoporins; Hikeshi; heat-shock proteins; nuclear transport receptor