title: Cardiac cell proliferation assessed by EdU, a novel analysis of cardiac regeneration.
abstract: Emerging evidence suggests that mammalian hearts maintain the capacity for cardiac regeneration. Rapid and sensitive identification of cardiac cellular proliferation is prerequisite for understanding the underlying mechanisms and strategies of cardiac regeneration. The following immunologically related markers of cardiac cells were analyzed: cardiac transcription factors Nkx2.5 and Gata 4; specific marker of cardiomyocytes TnT; endothelial cell marker CD31; vascular smooth muscle marker smooth muscle myosin IgG; cardiac resident stem cells markers IsL1, Tbx18, and Wt1. Markers were co-localized in cardiac tissues of embryonic, neonatal, adult, and pathological samples by 5-ethynyl-2'-deoxyuridine (EdU) staining. EdU was also used to label isolated neonatal cardiomyocytes in vitro. EdU robustly labeled proliferating cells in vitro and in vivo, co-immunostaining with different cardiac cells markers. EdU can rapidly and sensitively label proliferating cardiac cells in developmental and pathological states. Cardiac cell proliferation assessed by EdU is a novel analytical tool for investigating the mechanism and strategies of cardiac regeneration in response to injury.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China. zbch_600@163.com.|Department of Developmental and Regenerative Biology, Mount sinai school of medicine, New York, NY, 10029, USA. zbch_600@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China.
authors.forename: Suiyang
authors.initials: S
authors.lastname: Tong
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Hei Longjiang, People's Republic of China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China. xiahao1966@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Xia
keywords: Cardiac cells; EdU; Injury; Proliferation; Regeneration