title: Externally controlled triggered-release of drug from PLGA micro and nanoparticles.
abstract: Biofilm infections are extremely hard to eradicate and controlled, triggered and controlled drug release properties may prolong drug release time. In this study, the ability to externally control drug release from micro and nanoparticles was investigated. We prepared micro/nanoparticles containing ciprofloxacin (CIP) and magnetic nanoparticles encapsulated in poly (lactic-co-glycolic acid) PLGA. Both micro/nanoparticles were observed to have narrow size distributions. We investigated and compared their passive and externally triggered drug release properties based on their different encapsulation structures for the nano and micro systems. In passive release studies, CIP demonstrated a fast rate of release in first 2 days which then slowed and sustained release for approximately 4 weeks. Significantly, magnetic nanoparticles containing systems all showed ability to have triggered drug release when exposed to an external oscillating magnetic field (OMF). An experiment where the OMF was turned on and off also confirmed the ability to control the drug release in a pulsatile manner. The magnetically triggered release resulted in a 2-fold drug release increase compared with normal passive release. To confirm drug integrity following release, the antibacterial activity of released drug was evaluated in Pseudomonas aeruginosa biofilms in vitro. CIP maintained its antimicrobial activity after encapsulation and triggered release.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Science, Harbin 150001, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Hua
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin, PR China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Tan
authors.affiliation: College of Pharmacy, The University of Texas at Austin, 1 University Station, A1920, Austin, TX 78712, United States of America.
authors.forename: H M H N
authors.initials: HM
authors.lastname: Bandara
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Northeast Forestry University, Harbin, PR China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Science, Harbin 150001, China.
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Pharmacy, The University of Texas at Austin, 1 University Station, A1920, Austin, TX 78712, United States of America.
authors.forename: Hugh D C
authors.initials: HD
authors.lastname: Smyth
mesh_terms: D018441:Biofilms / Q000187:drug effects; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000737:chemistry; D003692:Delayed-Action Preparations / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000472:pathogenicity
chemical_list: D003692:Delayed-Action Preparations; D058185:Magnetite Nanoparticles; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002939:Ciprofloxacin