title: Bioconversion of agro-industrial by-products in rhamnolipids toward applications in enhanced oil recovery and bioremediation.
abstract: In this work, biosurfactant production by a Pseudomonas aeruginosa strain was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/l) was obtained using a culture medium containing corn steep liquor (10% (v/v)) and molasses (10% (w/v)). The biosurfactant reduced the surface tension of water up to 30 mN/m, and exhibited a high emulsifying activity (E24=60%), with a critical micelle concentration as low as 50 mg/l. The biosurfactant produced in this alternative medium was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10. However, using LB medium, nine different rhamnolipid congeners were identified, being the most abundant the di-rhamnolipid Rha-Rha-C10-C10. The rhamnolipid mixture produced in the alternative medium exhibited a better performance in removing oil from contaminated sand when compared with two chemical surfactants, suggesting its potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation.
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal. Electronic address: egudina@deb.uminho.pt.
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Gudiña
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Rodrigues
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: Eliana
authors.initials: E
authors.lastname: Alves
authors.affiliation: Mass Spectrometry Centre, QOPNA, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
authors.forename: M Rosário
authors.initials: MR
authors.lastname: Domingues
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.affiliation: CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
authors.forename: Lígia R
authors.initials: LR
authors.lastname: Rodrigues
mesh_terms: D000383:Agriculture; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D003470:Culture Media; D004655:Emulsions; D006017:Glycolipids / Q000737:chemistry*; D007220:Industrial Waste / Q000032:analysis*; D008969:Molecular Sequence Data; D010578:Petroleum / Q000032:analysis*; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D003470:Culture Media; D004655:Emulsions; D006017:Glycolipids; D007220:Industrial Waste; D010578:Petroleum; D013501:Surface-Active Agents; C418382:rhamnolipid
keywords: Bioremediation; Corn steep liquor; Molasses; Pseudomonas aeruginosa; Rhamnolipid