title: Production of mouse monoclonal antibody against Streptococcus dysgalactiae GapC protein and mapping its conserved B-cell epitope.
abstract: Streptococcus dysgalactiae (S. dysgalactiae) GapC protein is a protective antigen that induces partial immunity against S. dysgalactiae infection in animals. To identify the conserved B-cell epitope of S. dysgalactiae GapC, a mouse monoclonal antibody 1E11 (mAb1E11) against GapC was generated and used to screen a phage-displayed 12-mer random peptide library (Ph.D.-12). Eleven positive clones recognized by mAb1E11 were identified, most of which matched the consensus motif TGFFAKK. Sequence of the motif exactly matched amino acids 97-103 of the S. dysgalactiae GapC. In addition, the epitope (97)TGFFAKK(103) showed high homology among different streptococcus species. Site-directed mutagenic analysis further confirmed that residues G98, F99, F100 and K103 formed the core of (97)TGFFAKK(103), and this core motif was the minimal determinant of the B-cell epitope recognized by the mAb1E11. Collectively, the identification of conserved B-cell epitope within S. dysgalactiae GapC highlights the possibility of developing the epitope-based vaccine.
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Limeng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Fan
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Liquan
authors.initials: L
authors.lastname: Yu
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Hunan
authors.initials: H
authors.lastname: Sun
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wu
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Yongzhong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Baifen
authors.initials: B
authors.lastname: Song
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Ma
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Tong
authors.affiliation: College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China.
authors.forename: Zhanbo
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: College of Life Science and Technology, HeiLongJiang BaYi Agricultural University, Daqing 163319, China; College of Animal Science and Veterinary Medicine, HeiLongJiang BaYi Agricultural University, Daqing 163319, China. Electronic address: cuiyudong@yahoo.com.
authors.forename: Yudong
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D013045:Species Specificity; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary; D022541:Streptococcal Vaccines / Q000276:immunology; D013291:Streptococcus / Q000145:classification / Q000276:immunology*; D017108:Streptococcus Phages / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018985:Epitopes, B-Lymphocyte; C469817:GapC protein, Streptococcus; D022541:Streptococcal Vaccines
keywords: Epitope; GapC; Homology; Phage display; Streptococcus dysgalactiae