title: Prevalence of human papillomavirus in laryngeal and hypopharyngeal squamous cell carcinomas in northern Spain.
abstract: BACKGROUND
  Recent studies support a role for human papillomavirus (HPV) in oropharyngeal squamous cell carcinomas (SCCs); however, the significance of HPV in non-oropharyngeal head and neck cancers is uncertain. The aim of this study was to determine the prevalence of HPV in a large cohort of laryngeal and hypopharyngeal SCCs in northern Spain.
  
  
  METHODS
  Clinical records and paraffin-embedded tumor specimens of 124 consecutive patients surgically treated for laryngeal (62 cases) and hypopharyngeal (62 cases) SCCs between 2002 and 2007 were retrieved. All cases were histologically evaluated, and presence of HPV was assessed by p16-immunohistochemistry followed by GP5+/6+-PCR-based DNA detection. Samples positive in both assays were subjected to HPV genotyping and HPV E6 transcript analysis.
  
  
  RESULTS
  Seventeen cases (14%) were positive for p16 immunostaining, of which 2 (1 larynx, 1 hypopharynx, 1.6% of total series) were found positive for HPV DNA by subsequent GP5+6+-PCR. Both SCCs contained HPV type 16 and showed HPV16 E6 mRNA expression.
  
  
  CONCLUSIONS
  HPV is only occasionally involved in laryngeal and hypopharyngeal SCC patients in northern Spain.
authors.affiliation: Department of Otolaryngology, IUOPA, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain. Electronic address: juanpablo.rodrigo@sespa.princast.es.
authors.forename: Juan P
authors.initials: JP
authors.lastname: Rodrigo
authors.affiliation: Department of Otolaryngology, IUOPA, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Hermsen
authors.affiliation: Department of Pathology, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain.
authors.forename: Manuel F
authors.initials: MF
authors.lastname: Fresno
authors.affiliation: Department of Otolaryngology/Head and Neck Surgery, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Ruud H
authors.initials: RH
authors.lastname: Brakenhoff
authors.affiliation: Department of Otolaryngology, IUOPA, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain.
authors.forename: Fabian
authors.initials: F
authors.lastname: García-Velasco
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Peter J F
authors.initials: PJ
authors.lastname: Snijders
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Daniëlle A M
authors.initials: DA
authors.lastname: Heideman
authors.affiliation: Department of Otolaryngology, IUOPA, Hospital Universitario Central de Asturias, University of Oviedo, Oviedo, Spain.
authors.forename: Juana M
authors.initials: JM
authors.lastname: García-Pedrero
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000453:epidemiology* / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006258:Head and Neck Neoplasms / Q000453:epidemiology* / Q000821:virology; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000453:epidemiology* / Q000821:virology; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000453:epidemiology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012097:Repressor Proteins / Q000235:genetics; D012189:Retrospective Studies; D013030:Spain / Q000453:epidemiology; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins
keywords: Human papillomavirus; Hypopharynx; Immunohistochemistry; In situ hybridization; Larynx; PCR; Squamous cell carcinoma