title: The utilization of SiNWs/AuNPs-modified indium tin oxide (ITO) in fabrication of electrochemical DNA sensor.
abstract: This work describes the incorporation of SiNWs/AuNPs composite as a sensing material for DNA detection on indium tin-oxide (ITO) coated glass slide. The morphology of SiNWs/AuNPs composite as the modifier layer on ITO was studied by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The morphological studies clearly showed that SiNWs were successfully decorated with 20 nm-AuNPs using self-assembly monolayer (SAM) technique. The effective surface area for SiNWs/AuNPs-modified ITO enhanced about 10 times compared with bare ITO electrode. SiNWs/AuNPs nanocomposite was further explored as a matrix for DNA probe immobilization in detection of dengue virus as a bio-sensing model to evaluate its performance in electrochemical sensors. The hybridization of complementary DNA was monitored by differential pulse voltammetry (DPV) using methylene blue (MB) as the redox indicator. The fabricated biosensor was able to discriminate significantly complementary, non-complementary and single-base mismatch oligonucleotides. The electrochemical biosensor was sensitive to target DNA related to dengue virus in the range of 9.0-178.0 ng/ml with detection limit of 3.5 ng/ml. In addition, SiNWs/AuNPs-modified ITO, regenerated up to 8 times and its stability was up to 10 weeks at 4°C in silica gel.
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Chemistry and Biology, Centre for Defense Foundation Studies, National Defense University of Malaysia, Sungai Besi Camp, 57000 Kuala Lumpur, Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Jahwarhar Izuan Abdul
authors.initials: JI
authors.lastname: Rashid
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: azahy@upm.edu.my.
authors.forename: Nor Azah
authors.initials: NA
authors.lastname: Yusof
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Jaafar
authors.initials: J
authors.lastname: Abdullah
authors.affiliation: Institute of Nanoelectronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
authors.forename: Uda
authors.initials: U
authors.lastname: Hashim
authors.affiliation: Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: rezahajian@upm.edu.my.
authors.forename: Reza
authors.initials: R
authors.lastname: Hajian
mesh_terms: D015374:Biosensing Techniques; D015342:DNA Probes / Q000737:chemistry / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis*; D003716:Dengue Virus / Q000235:genetics; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D061925:Immobilized Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D008751:Methylene Blue / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053770:Nanowires / Q000737:chemistry*; D010084:Oxidation-Reduction; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012825:Silicon / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D061925:Immobilized Nucleic Acids; D012367:RNA, Viral; D017971:Tin Compounds; C109984:indium tin oxide; D006046:Gold; D008751:Methylene Blue; D012825:Silicon
keywords: Electrochemical DNA sensor; Gold nanoparticles (AuNPs); Hybridization; Indium tin oxide (ITO); Silicon nanowires (SiNWs)