title: Implantation of an ultrafiltration device in the ileum and spiral colon of steers to continuously collect intestinal fluid.
abstract: Collection of fluid from the lumen of the gastrointestinal tract is commonly necessary for research projects, but presents challenges including intestinal motility and potential for leakage of intestinal contents. In this study, ultrafiltration collection devices were surgically implanted in the ileum and spiral colon of 12 steers for repeated collection of intestinal fluid over 48 hours. There were no significant complications associated with surgery or during the post-operative period, nor were there any significant pathologic changes found at necropsy 3 or 4 days post-surgery. Over 48 hours, we obtained 88% of the desired 212 samples. Only two devices failed to routinely collect samples. Use of ultrafiltration probes is a novel, consistent and humane method to repeatedly sample the gastrointestinal contents.
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Chelsea D
authors.initials: CD
authors.lastname: Warren
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Timo
authors.initials: T
authors.lastname: Prange
authors.affiliation: Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Nigel B
authors.initials: NB
authors.lastname: Campbell
authors.affiliation: Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Mat P
authors.initials: MP
authors.lastname: Gerard
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Luke G
authors.initials: LG
authors.lastname: Martin
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Jacob
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Geof W
authors.initials: GW
authors.lastname: Smith
authors.affiliation: Department of Molecular and Biomedical Sciences, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States.
authors.forename: Mark G
authors.initials: MG
authors.lastname: Papich
authors.affiliation: Department of Population Health and Pathobiology, College of Veterinary Medicine, NC State University, 1060 William Moore Drive, Raleigh, NC 27607, United States. Electronic address: derek_foster@ncsu.edu.
authors.forename: Derek M
authors.initials: DM
authors.lastname: Foster
mesh_terms: D000818:Animals; D001826:Body Fluids; D002417:Cattle; D003106:Colon / Q000601:surgery*; D007082:Ileum / Q000601:surgery*; D007419:Intestinal Secretions; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*; D014462:Ultrafiltration / Q000295:instrumentation*
keywords: Bovine; Gastrointestinal fluid; Surgery; Ultrafiltration