title: [Development of an immunochromatographic test system for the detection of human enidermal growth factor].
abstract: A method was developed for the rapid detection of human epidermal growth factor based on a sandwich-format immunochromatographic assay. The contact between the sample and the test strip with immobilized immunoreagents initiates the fluid flow movement across the membrane components of the test strip, immunochemical reactions, and the formation of colored lines. Requirements on the configuration of the test system in order to achieve the lowest limit of detection were defined in the course of the development of the assay. It was shown that this method enables the detection of human epidermal growth factor within 5 min at concentrations as low as 10 pg/mL in aqueous solutions, urine, and the blood serum and plasma. The developed test system can be used for point-of-care diagnostics.
authors.forename: N A
authors.initials: NA
authors.lastname: Byzova
authors.forename: L N
authors.initials: LN
authors.lastname: Lukhverchik
authors.forename: A V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: N V
authors.initials: NV
authors.lastname: Piven'
authors.forename: A I
authors.initials: AI
authors.lastname: Burakovskiĭ
authors.forename: B B
authors.initials: BB
authors.lastname: Dzantiev
mesh_terms: D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D004815:Epidermal Growth Factor / Q000097:blood* / Q000652:urine*; D006801:Humans; D008567:Membranes, Artificial; D012680:Sensitivity and Specificity
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D008567:Membranes, Artificial; D004815:Epidermal Growth Factor