title: Conditioned medium of adipose-derived stromal cell culture in three-dimensional bioreactors for enhanced wound healing.
abstract: BACKGROUND
  It was previously shown that human adipose-derived stromal cell (hADSC)-conditioned medium (CM) promotes wound healing. An essential part of the wound healing process is neovascularization in the wound bed.
  
  
  METHODS
  We hypothesized that CM prepared from hADSCs cultured as spheroids in three-dimensional suspension bioreactors (spheroid CM) would contain much higher concentrations of angiogenic growth factors secreted by hADSCs, induce a higher extent of neovascularization in the wound bed, and improve wound healing as compared with CM prepared by conventional monolayer culture (monolayer CM).
  
  
  RESULTS
  The concentrations of angiogenic growth factors (i.e., vascular endothelial growth factor, basic fibroblast growth factor, and hepatocyte growth factor) in spheroid CM were 20- to 145-fold higher than those in monolayer CM. Either fresh medium, monolayer CM, or spheroid CM was administered to full-thickness wounds created on the dorsal aspects of athymic mice. The monolayer CM promoted wound healing as compared with fresh medium or no treatment. Importantly, wound closure was faster, and dermal and epidermal regeneration was improved in the spheroid CM-treated mice compared with that in the monolayer CM-treated mice.
  
  
  CONCLUSIONS
  The improved wound healing by spheroid CM may be attributed, at least in part, to enhanced neovascularization in the wound beds. The spheroid-based CM approach showed potential as a therapy for skin wound repair.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sun Hyun
authors.initials: SH
authors.lastname: Kwon
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Republic of Korea.
authors.forename: Suk Ho
authors.initials: SH
authors.lastname: Bhang
authors.affiliation: Interdisciplinary Program for Bioengineering, College of Engineering, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hyeon-Ki
authors.initials: HK
authors.lastname: Jang
authors.affiliation: Department of Bioengineering, College of Engineering, Hanyang University, Seoul, Republic of Korea. Electronic address: rhim@hanyang.ac.kr.
authors.forename: Taiyoun
authors.initials: T
authors.lastname: Rhim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea; Institute of Bioengineering, Institute of Chemical Processess, Seoul National University, Seoul, Republic of Korea. Electronic address: byungskim@snu.ac.kr.
authors.forename: Byung-Soo
authors.initials: BS
authors.lastname: Kim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D019149:Bioreactors; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic; D018874:Spheroids, Cellular; D017154:Stromal Cells / Q000502:physiology*; D014945:Wound Healing
chemical_list: D017077:Culture Media, Conditioned
keywords: Conditioned medium; Human adipose-derived stromal cells; Neovascularization; Wound healing