title: The protoxin Cry1Ac of Bacillus thuringiensis improves the protection conferred by intranasal immunization with Brucella abortus RB51 in a mouse model.
abstract: Brucellosis is a zoonotic disease affecting many people and animals worldwide. Preventing this infection requires improving vaccination strategies. The protoxin Cry1Ac of Bacillus thuringiensis is an adjuvant that, in addition to increasing the immunogenicity of different antigens, has shown to be protective in different models of parasitic infections. The objective of the present study was to test whether the intranasal co-administration of pCry1Ac with the RB51 vaccine strain of Brucella abortus confers protection against an intranasal challenge with the virulent strain B. abortus 2308 in BALB/c mice. The results showed that co-administration of pCry1Ac and RB51, increased the immunoprotection conferred by the vaccine as evidenced by the following: (1) decrease of the splenic bacterial load when challenged intranasally with the virulent strain; (2) greater in vivo cytotoxic activity in response to the transference of previously infected cells; (3) further proliferation of cytotoxic TCD8+ cells in response to stimulation with heat-inactivated bacteria; (4) increased production of TNF-α and IFN-γ; and (5) significant IgG2a response. These results indicate that the use of the Cry1Ac protein as a mucosal adjuvant via the intranasal route can be a promising alternative for improving current RB51 vaccine against brucellosis.
authors.affiliation: Laboratorio de Inmunidad en Mucosas, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, Tlalnepantla 54090, Estado de México, Mexico. Electronic address: ediqbp@hotmail.com.
authors.forename: Edith
authors.initials: E
authors.lastname: González-González
authors.affiliation: Laboratorio de Inmunidad en Mucosas, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, Tlalnepantla 54090, Estado de México, Mexico. Electronic address: cd_an@hotmail.com.
authors.forename: Ana Lilia
authors.initials: AL
authors.lastname: García-Hernández
authors.affiliation: Laboratorio de Inmunología Médica, Escuela Superior de Medicina, Instituto Politécnico Nacional, Unidad Profesional Lázaro Cárdenas, Avenida Salvador Díaz Mirón s/n esquina Plan de San Luis, Col. Santo Tomás, Delegación Miguel Hidalgo 11340, Distrito Federal, Mexico. Electronic address: biodino@yahoo.com.mx.
authors.forename: Raúl
authors.initials: R
authors.lastname: Flores-Mejía
authors.affiliation: Laboratorio de Inmunología Celular Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Unidad Profesional Lázaro Cárdenas, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Delegación Miguel Hidalgo 11340, Distrito Federal, Mexico. Electronic address: rlopezsantiago@hotmail.com.
authors.forename: Rubén
authors.initials: R
authors.lastname: López-Santiago
authors.affiliation: Laboratorio de Inmunidad en Mucosas, Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Avenida de los Barrios 1, Los Reyes Iztacala, Tlalnepantla 54090, Estado de México, Mexico. Electronic address: lemofi@unam.mx.
authors.forename: Leticia
authors.initials: L
authors.lastname: Moreno-Fierros
mesh_terms: D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000494:pharmacology*; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000276:immunology*; D002003:Brucella abortus / Q000276:immunology; D002006:Brucellosis / Q000517:prevention & control*; D004731:Endotoxins / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology*; D007114:Immunization; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D002004:Brucella Vaccine; D004731:Endotoxins; D006460:Hemolysin Proteins; D007074:Immunoglobulin G; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Adjuvants; Brucellosis; Cry1Ac protoxin; Intranasal vaccine; RB51 vaccine