title: Inhibitory effects of dobutamine on human gastric adenocarcinoma.
abstract: OBJECTIVE
  To explore the inhibitory effects of dobutamine on gastric adenocarcinoma cells.
  
  
  METHODS
  Dobutamine was used to treat gastric adenocarcinoma cells (SGC-7901) and cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of dobutamine combined with cisplatin on cell viability were also analyzed. Cell migration was studied using the wound healing assay, and cell proliferation was analyzed using the colony formation assay. A cell invasion assay was carried out using Transwell cell culture chambers. The cell cycle and cell apoptosis were analyzed by flow cytometry. Western blot and immunocytochemistry were performed to determine the expression of Yes-associated protein (YAP) in treated cells.
  
  
  RESULTS
  Dobutamine significantly inhibited cell growth, migration, cell colony formation, and cell invasion into Matrigel. Dobutamine also arrested the cell cycle at G1/S phase, and increased the rate of apoptosis of gastric adenocarcinoma cells. The expression of YAP was detected mainly in the nucleus in the absence of dobutamine. However, reduced expression of phosphorylated YAP was mainly found in the cytosol following treatment with dobutamine.
  
  
  CONCLUSIONS
  Dobutamine has significant inhibitory effects on gastric adenocarcinoma cells and may be used in neoadjuvant therapy not only for gastric cancer, but also for other tumors.
authors.affiliation: Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.
authors.forename: Hui-Xia
authors.initials: HX
authors.lastname: Zheng
authors.affiliation: Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Wu
authors.affiliation: Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Du
authors.affiliation: Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China.
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Liang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004280:Dobutamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects; D006801:Humans; D009361:Neoplasm Invasiveness; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D010750:Phosphoproteins; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C088374:YAP1 protein, human; D004280:Dobutamine; D002945:Cisplatin
keywords: Dobutamine; Gastric adenocarcinoma cells; Hippo pathway; Human gastric adenocarcinoma cell line SGC-7901; Therapy; Yes-associated protein