title: The tight junction protein JAM-A functions as coreceptor for rotavirus entry into MA104 cells.
abstract: Several molecules have been identified as receptors or coreceptors for rotavirus infection, including glycans, integrins, and hsc70. In this work we report that the tight junction proteins JAM-A, occludin, and ZO-1 play an important role during rotavirus entry into MA104 cells. JAM-A was found to function as coreceptor for rotavirus strains RRV, Wa, and UK, but not for rotavirus YM. Reassortant viruses derived from rotaviruses RRV and YM showed that the virus spike protein VP4 determines the use of JAM-A as coreceptor.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México. Electronic address: jmtorres@ibt.unam.mx.
authors.forename: Jesús M
authors.initials: JM
authors.lastname: Torres-Flores
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México. Electronic address: dsilva@ibt.unam.mx.
authors.forename: Daniela
authors.initials: D
authors.lastname: Silva-Ayala
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México. Electronic address: maet@ibt.unam.mx.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Espinoza
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México. Electronic address: susana@ibt.unam.mx.
authors.forename: Susana
authors.initials: S
authors.lastname: López
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México. Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, México. Electronic address: arias@ibt.unam.mx.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Arias
mesh_terms: D000818:Animals; D002460:Cell Line; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D034622:RNA Interference; D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D012401:Rotavirus / Q000502:physiology*; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D034741:RNA, Small Interfering; D011956:Receptors, Cell Surface; D062725:Tight Junction Proteins
keywords: JAM-A; Occludin; Rotavirus; Tight junction proteins; Virus entry; ZO-1