title: PLCγ1 participates in protein transport and diacylglycerol production triggered by cargo arrival at the Golgi.
abstract: Diacylglycerol (DAG) is required for membrane traffic and structural organization at the Golgi. DAG is a lipid metabolite of several enzymatic reactions present at this organelle, but the mechanisms by which they are regulated are still unknown. Here, we show that cargo arrival at the Golgi increases the recruitment of the DAG-sensing constructs C1-PKCθ-GFP and the PKD-wt-GFP. The recruitment of both constructs was reduced by PLCγ1 silencing. Post-Golgi trafficking of transmembrane and soluble proteins was impaired in PLCγ1-silenced cells. Under basal conditions, PLCγ1 contributed to the maintenance of the pool of DAG associated with the Golgi and to the structural organization of the organelle. Finally, we show that cytosolic phospholipase C (PLC) can hydrolyse phosphatidylinositol 4-phosphate in isolated Golgi membranes. Our results indicate that PLCγ1 is part of the molecular mechanism that couples cargo arrival at the Golgi with DAG production to co-ordinate the formation of transport carriers for post-Golgi traffic.
authors.affiliation: Departament de Biologia Cel·lular, Immunologia i Neurociències, Facultat de Medicina, Universitat de Barcelona, C/ Casanova, 143, 08036, Barcelona, Spain; Current address: Vlaams Instituut voor Biotechnologie (VIB), Centre for the Biology of Disease and KU Leuven, Department of Human Genetics, Campus Gasthuisberg, 3000 Leuven, Belgium.
authors.forename: Adrià
authors.initials: A
authors.lastname: Sicart
authors.forename: Matilda
authors.initials: M
authors.lastname: Katan
authors.forename: Gustavo
authors.initials: G
authors.lastname: Egea
authors.forename: Elisabet
authors.initials: E
authors.lastname: Sarri
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism* / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D051966:Phospholipase C gamma / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology*; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D004075:Diglycerides; D018129:Phosphatidylinositol Phosphates; C037178:phosphatidylinositol 4-phosphate; D011493:Protein Kinase C; D010738:Type C Phospholipases; C522157:PLCG1 protein, human; D051966:Phospholipase C gamma
keywords: Golgi; diacylglycerol; membrane trafficking; phospholipase C; protein kinase D