title: One-staged aptamer-based isolation and application of endothelial progenitor cells in a porcine myocardial infarction model.
abstract: A multitude of stem cell types has been extensively studied and used for myocardial regenerative therapy. Amongst these endothelial progenitor cells form a promising source. In our present study, we investigated a one-staged approach for isolation and application of autologous endothelial progenitor cells in a pig model of myocardial infarction. Endothelial progenitor cell isolation was performed by immediately preprocedural bone marrow aspiration and consecutive positive selection by aptamer-based magnetic cell sorting. Animals were divided in three groups receiving endothelial progenitor cells, saline, or no intramyocardial injection respectively. Postprocedural follow-up consisted of weekly echocardiographic evaluations. Postmortem histological analysis after four weeks focused on detection of transplanted PKH26-positive endothelial progenitor cells and neovascularization within the infarcted myocardium. A significant difference in left ventricular ejection fraction could not be shown between the three groups. PKH26-stained endothelial progenitor cells could be found in the endothelial progenitor cells transplanted group, although detection was scarce. Large-sized capillaries were found to be significantly more in endothelial progenitor cells treated myocardium. The one-stage approach of endothelial progenitor cells isolation and application presented herein offers a new therapeutic concept. Even though a beneficial impact on myocardial function could not be assessed, increased neovascularization may indicate positive effects on remodeling processes. Being able to harvest endothelial progenitor cells right before application provides a wider scope of action in urgent cases.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen , Tuebingen, Germany .
authors.forename: Christoph
authors.initials: C
authors.lastname: Haller
authors.forename: Bianka
authors.initials: B
authors.lastname: Sobolewska
authors.forename: David
authors.initials: D
authors.lastname: Schibilsky
authors.forename: Meltem
authors.initials: M
authors.lastname: Avci-Adali
authors.forename: Christian
authors.initials: C
authors.lastname: Schlensak
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Wendel
authors.forename: Tobias
authors.initials: T
authors.lastname: Walker
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D003331:Coronary Vessels / Q000503:physiopathology; D066026:Endothelial Progenitor Cells / Q000637:transplantation*; D005260:Female; D008297:Male; D015335:Molecular Probes; D009203:Myocardial Infarction / Q000628:therapy*; D018919:Neovascularization, Physiologic; D015341:Nucleic Acid Probes / Q000737:chemistry; D044968:Regenerative Medicine; D013194:Staining and Labeling; D034421:Sus scrofa
chemical_list: D052157:Aptamers, Nucleotide; D015335:Molecular Probes; D015341:Nucleic Acid Probes