title: Isolation, identification and functional profile of excretory-secretory peptides from Onchocerca ochengi.
abstract: Parasitic helminths excrete or secrete a variety of functional molecules into the internal milieu of their mammalian hosts and arthropod vectors which reveal distinct immunomodulatory and other biological activities. We identified and initially characterized the low molecular weight peptide composition of the secretome from the filarial parasite Onchocerca ochengi. A total of 85 peptides were purified by liquid chromatography and further characterized by mass spectrometry. 72 of these peptides were derived from already described Onchocerca proteins and 13 peptide sequences are included in the sequence of uncharacterized proteins. Three peptides, similar to host defense peptides, revealed antibacterial activity. The present analysis confirms the putative involvement of low molecular weight compounds in the parasite-host cross-talk.
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestr. 85, Build. 22a, 22603 Hamburg, Germany.
authors.forename: Raphael
authors.initials: R
authors.lastname: Eberle
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany.
authors.forename: Norbert W
authors.initials: NW
authors.lastname: Brattig
authors.affiliation: Institute of Organic Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, 20146 Hamburg, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Trusch
authors.affiliation: Institute of Clinical Chemistry, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Schlüter
authors.affiliation: Institute of Agricultural Research for Development, Veterinary Research Laboratory, Wakwa Centre, PO Box 65, Ngaoundéré, Cameroon.
authors.forename: Mbunkah Daniel
authors.initials: MD
authors.lastname: Achukwi
authors.affiliation: Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
authors.forename: Albert
authors.initials: A
authors.lastname: Eisenbarth
authors.affiliation: Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
authors.forename: Alfons
authors.initials: A
authors.lastname: Renz
authors.affiliation: Institute of Animal Physiology, University of Münster, Schlossplatz 8, 48143 Münster, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Liebau
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestr. 85, Build. 22a, 22603 Hamburg, Germany; Institute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), 20246 Hamburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Perbandt
authors.affiliation: Institute of Biochemistry and Molecular Biology, University of Hamburg, Laboratory of Structural Biology of Infection and Inflammation, c/o DESY, Notkestr. 85, Build. 22a, 22603 Hamburg, Germany. Electronic address: Christian.Betzel@uni-hamburg.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Betzel
mesh_terms: D000818:Animals; D003429:Cross Reactions; D005260:Female; D015801:Helminth Proteins / Q000378:metabolism*; D009854:Onchocerca / Q000502:physiology*; D009855:Onchocerciasis / Q000517:prevention & control / Q000635:transmission*; D012843:Simuliidae / Q000469:parasitology
chemical_list: D015801:Helminth Proteins
keywords: Antibacterial activity; Excretory–secretory products; Filaria; Functional peptides; Onchocerca