title: Overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest in PC12 cell line.
abstract: LIM-homeobox genes play a pivotal function in tissue patterning and differentiation, Lhx8 is a member of LIM-homeobox gene family, and it is selectively expressed in embryonic basal forebrain and is a key factor for the determination of cholinergic cells fate. However, besides cholinergic differentiation, little is known about the potential role of Lhx8 in cell biology. In this study, we transfected Lhx8 complementary DNA (cDNA) into PC12 cell line using lentiviral vectors to acquire the cells which stably expressed high level of Lhx8, and we provide the experimental evidence that overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest but not apoptosis in vitro. In conclusion, besides cholinergic differentiation, our results suggest that Lhx8 also plays as a suppressor gene of proliferation in cell biology.
authors.affiliation: Department of Human Anatomy, the Jiangsu Key Laboratory of Neuroregeneration, Medical School of Nantong University, 19 Qixiu Road, Nantong, 226001, Jiangsu, China.
authors.forename: Haoming
authors.initials: H
authors.lastname: Li
authors.forename: Jianbing
authors.initials: J
authors.lastname: Qin
authors.forename: Guohua
authors.initials: G
authors.lastname: Jin
authors.forename: Linqing
authors.initials: L
authors.lastname: Zou
authors.forename: Jinhong
authors.initials: J
authors.lastname: Shi
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Xiang
authors.initials: X
authors.lastname: Cheng
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D016153:Genes, Suppressor; D049452:Green Fluorescent Proteins / Q000235:genetics; D060850:LIM-Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D016716:PC12 Cells / Q000473:pathology*; D051381:Rats; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D018076:DNA, Complementary; C112960:LIM homeobox protein 8; D060850:LIM-Homeodomain Proteins; D014157:Transcription Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins