title: The eukaryotic elongation factor 1A is critical for genome replication of the paramyxovirus respiratory syncytial virus.
abstract: The eukaryotic translation factor eEF1A assists replication of many RNA viruses by various mechanisms. Here we show that down-regulation of eEF1A restricts the expression of viral genomic RNA and the release of infectious virus, demonstrating a biological requirement for eEF1A in the respiratory syncytial virus (RSV) life cycle. The key proteins in the replicase/transcriptase complex of RSV; the nucleocapsid (N) protein, phosphoprotein (P) and matrix (M) protein, all associate with eEF1A in RSV infected cells, although N is the strongest binding partner. Using individually expressed proteins, N, but not P or M bound to eEF1A. This study demonstrates a novel interaction between eEF1A and the RSV replication complex, through binding to N protein, to facilitate genomic RNA synthesis and virus production.
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia.
authors.forename: Ting
authors.initials: T
authors.lastname: Wei
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Li
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia; Clinical Medical Virology Centre, The University of Queensland, Herston, Australia; Sir Albert Sakzewski Virus Research Centre, Childrens Health Queensland, Herston, Australia.
authors.forename: Daneth
authors.initials: D
authors.lastname: Marcial
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia; Sir Albert Sakzewski Virus Research Centre, Childrens Health Queensland, Herston, Australia.
authors.forename: Moshin
authors.initials: M
authors.lastname: Khan
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia.
authors.forename: Min-Hsuan
authors.initials: MH
authors.lastname: Lin
authors.affiliation: Clinical Medical Virology Centre, The University of Queensland, Herston, Australia; Sir Albert Sakzewski Virus Research Centre, Childrens Health Queensland, Herston, Australia.
authors.forename: Natale
authors.initials: N
authors.lastname: Snape
authors.affiliation: Centre for Research in Therapeutic Solutions, University of Canberra, Canberra, Australia.
authors.forename: Reena
authors.initials: R
authors.lastname: Ghildyal
authors.affiliation: Queensland Institute of Medical Research Berghofer, Herston, Australia; Australian Infectious Disease Research Centre, St Lucia, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Harrich
authors.affiliation: Clinical Medical Virology Centre, The University of Queensland, Herston, Australia; Sir Albert Sakzewski Virus Research Centre, Childrens Health Queensland, Herston, Australia; Australian Infectious Disease Research Centre, St Lucia, Australia.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Spann
mesh_terms: D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016679:Genome, Viral / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D018357:Respiratory Syncytial Virus Infections / Q000378:metabolism*; D012136:Respiratory Syncytial Viruses / Q000502:physiology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000502:physiology*
chemical_list: D020648:Peptide Elongation Factor 1; D014764:Viral Proteins