title: Use of in vivo induced antigen technology to identify genes from Aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout Oncorhynchus mykiss.
abstract: BACKGROUND
  Aeromonas salmonicida is a major fish pathogen associated with mass mortalities in salmonid fish. In the present study, we applied In Vivo Induced Antigen Technology (IVIAT), a technique that relies on antibodies adsorbed against in vitro cultures of the pathogen, to a clinical isolate of A. salmonicida subsp. salmonicida.
  
  
  RESULTS
  The results from IVIAT allowed identification of four proteins that were upregulated in the fish samples: A UDP-3-O-acyl-N-acetylglucosamine deacetylase, an RNA polymerase sigma factor D as well as TonB and a hypothetical protein. Subsequent investigations were performed using real-time PCR and cDNA synthesised from infected spleen, liver and anterior kidneys. These confirmed that the transcription level of each of these genes was significantly upregulated during the infection process compared to bacteria in vitro.
  
  
  CONCLUSIONS
  The present studied identified four genes that were upregulated during the infectious process and are likely to play a role in the virulence of A. salmonicida. Because these are antigenic they might constitute potential targets for the development of new vaccine as well as therapeutic agents.
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinarplatz 1, Vienna, 1210, Austria. menanteaus@staff.vetmeduni.ac.at.
authors.forename: Simon
authors.initials: S
authors.lastname: Menanteau-Ledouble
authors.affiliation: Fish Medicine and Managements, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, 71515, Assiut, Egypt. hatemtoughan@hotmail.com.
authors.forename: Hatem
authors.initials: H
authors.lastname: Soliman
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinarplatz 1, Vienna, 1210, Austria. Gokhlesh.Kumar@vetmeduni.ac.at.
authors.forename: Gokhlesh
authors.initials: G
authors.lastname: Kumar
authors.affiliation: Clinical Division of Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinarplatz 1, Vienna, 1210, Austria. Mansour.El-Matbouli@vetmeduni.ac.at.
authors.forename: Mansour
authors.initials: M
authors.lastname: El-Matbouli
mesh_terms: D048409:Aeromonas salmonicida / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D000581:Amidohydrolases / Q000235:genetics / Q000502:physiology; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western / Q000662:veterinary; D005393:Fish Diseases / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D015854:Up-Regulation
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins; C057616:tonB protein, Bacteria; D000581:Amidohydrolases; C097307:UDP-3-O-acyl-N-acetylglucosamine deacetylase