title: Complete nucleotide sequence of the IncN plasmid encoding IMP-6 and CTX-M-2 from emerging carbapenem-resistant Enterobacteriaceae in Japan.
abstract: We have determined the DNA sequence of Klebsiella pneumoniae multidrug resistance plasmid pKPI-6, which is a self-transmissible IncN-type plasmid. pKPI-6 harboring blaIMP-6 and blaCTX-M-2 confers a stealth-type carbapenem resistance phenotype on members of the family Enterobacteriaceae that is not detectable with imipenem. pKPI-6 is already epidemic in Japan, favoring the dissemination of IMP-6 and CTX-M-2 in members of the family Enterobacteriaceae.
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan.
authors.forename: Shizuo
authors.initials: S
authors.lastname: Kayama
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan Department of Surgery I, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Shigemoto
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan Clinical Laboratory, Hiroshima General Hospital of West Japan Railway Company, Hiroshima, Hiroshima, Japan.
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Kuwahara
authors.affiliation: Center for Omics and Bioinformatics, The Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Kenshiro
authors.initials: K
authors.lastname: Oshima
authors.affiliation: Department of Plant Genome Research, Kazusa DNA Research Institute, Kisarazu, Chiba, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Hisatsune
authors.affiliation: Inserm U1092, Faculté de Médecine, Limoges, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Jové
authors.affiliation: Clinical Laboratory, Shiga Medical Center for Adults, Moriyama, Shiga, Japan.
authors.forename: Hisaaki
authors.initials: H
authors.lastname: Nishio
authors.affiliation: Department of Clinical Laboratory, Wakayama Rosai Hospital, Wakayama, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yamasaki
authors.affiliation: Clinical Laboratory, Hyogo Medical University Hospital, Nishinomiya, Hyogo, Japan.
authors.forename: Yasunao
authors.initials: Y
authors.lastname: Wada
authors.affiliation: Hyogo Prefectural Amagasaki Hospital, Amagasaki City, Hyogo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ueshimo
authors.affiliation: Hyogo Prefectural Amagasaki Hospital, Amagasaki City, Hyogo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Miura
authors.affiliation: Department of Surgery I, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan.
authors.forename: Taijiro
authors.initials: T
authors.lastname: Sueda
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Clinical Laboratory, Hiroshima University Hospital, Hiroshima City, Hiroshima, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Onodera
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Clinical Laboratory, Hiroshima University Hospital, Hiroshima City, Hiroshima, Japan.
authors.forename: Michiya
authors.initials: M
authors.lastname: Yokozaki
authors.affiliation: Center for Omics and Bioinformatics, The Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Masahira
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima City, Hiroshima, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohge
authors.affiliation: Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima City, Hiroshima, Japan Department of Bacteriology, Hiroshima University Graduate School of Biomedical Health Sciences, Hiroshima City, Hiroshima, Japan sugai@hiroshima-u.ac.jp/
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Sugai
mesh_terms: D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology*; D015378:Imipenem / Q000494:pharmacology; D007564:Japan; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D015378:Imipenem; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases