title: Biocompatibility of ultrafine grained zircaloy-2 produced by cryorolling for medical applications.
abstract: The present work deals with development of ultrafine grained zircaloy-2 and studying its potential for orthopedic application. The multimodal structure, i.e. the combination of coarse, ultrafine grained (UFG) and nanograined structures of zircaloy-2 is obtained by cryorolling the bulk alloy followed by annealing at 400 °C, and 450 °C for 30 min. An estimation of surface wettability of the alloy was obtained through contact angle measurement. The bioactivity of the alloy samples was investigated by incubating bone marrow derived stem cells. The cellular attachment, adhesion and proliferation at different intervals of incubation were characterized by scanning fluorescent microscopy and MTT assay. Cell culture results indicated that liquid nitrogen rolled alloy samples exhibited excellent in-vitro biocompatibility together with satisfactory bioactivity. Excellent genomic expressions were observed for zircaloy 2 processed by cryorolling.
authors.affiliation: Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India.
authors.forename: Pramanshu
authors.initials: P
authors.lastname: Trivedi
authors.affiliation: Department of Metallurgical and Materials Engineering, Indian Institute of Technology, Roorkee, India.
authors.forename: Sunkulp
authors.initials: S
authors.lastname: Goel
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Roorkee, India.
authors.forename: Snehasish
authors.initials: S
authors.lastname: Das
authors.affiliation: Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India; Department of Metallurgical and Materials Engineering, Indian Institute of Technology, Roorkee, India. Electronic address: rjayafmt@iitr.ernet.in.
authors.forename: R
authors.initials: R
authors.lastname: Jayaganthan
authors.affiliation: Centre of Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, India; Department of Metallurgical and Materials Engineering, Indian Institute of Technology, Roorkee, India.
authors.forename: Debrupa
authors.initials: D
authors.lastname: Lahiri
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Roorkee, India.
authors.forename: P
authors.initials: P
authors.lastname: Roy
mesh_terms: D000497:Alloys; D000818:Animals; D001672:Biocompatible Materials; D049109:Cell Proliferation; D051379:Mice; D008809:Mice, Inbred C3H; D046529:Microscopy, Electron, Transmission; D017155:Wettability; D014961:X-Ray Diffraction; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D015040:Zirconium
keywords: Bioactivity; Cryorolling; Microstructure; Wettability