title: Mixed ligand Cu(II)N2O2 complexes: biomimetic synthesis, activities in vitro and biological models, theoretical calculations.
abstract: Three new mixed ligand Cu(II)N2O2 complexes, namely, [Cu(II)(2-A-6-MBT)2(m-NB)2] (1), [Cu(II)(2-ABT)2(m-NB)2] (2), and [Cu(II)(2-ABT)2(o-NB)2] (3), (2-A-6-MBT = 2-amino-6-methoxybenzothiazole, m-NB = m-nitrobenzoate, 2-ABT = 2-aminobenzothiazole, and o-NB = o-nitrobenzoate), have been prepared by the biomimetic synthesis strategy, and their structures were determined by X-ray crystallography studies and spectral methods. These complexes exhibited the effective superoxide dismutase (SOD) activity and catecholase activity. On the basis of the experimental data and computational studies, the structure-activity relationship for these complexes was investigated. The results reveal that electron-accepting abilities of these complexes and coordination geometries have significant effects on the SOD activity and catecholase activity. Then, we found that 1 and 2 exerted potent intracellular antioxidant capacity in the model of H2O2-induced oxidative stress based on HeLa cervical cancer cells, which were screened out by the cytotoxicity assays of different kinds of cells. Furthermore, 1-3 showed the favorable biocompatibility in two different biological models: Saccharomyces cerevisiae and human vascular endothelial cells. These biological experimental data are indicative of the promising application potential of these complexes in biology and pharmacology.
authors.affiliation: Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University , Xi'an, Shaanxi 710069, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Yin
authors.forename: Yifan
authors.initials: Y
authors.lastname: Kang
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianli
authors.initials: J
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D052160:Benzothiazoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D032701:Biomimetics; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008958:Models, Molecular; D009573:Nitrites / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D009579:Nitrobenzoates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000737:chemistry*
chemical_list: C518437:2-nitrobenzoate; D000975:Antioxidants; D052160:Benzothiazoles; D056831:Coordination Complexes; D008024:Ligands; D009573:Nitrites; D009579:Nitrobenzoates; C025117:hyponitrite; D003300:Copper; D013482:Superoxide Dismutase; C025103:2-amino-6-methoxybenzothiazole