title: Biogenic silver nanoparticles associated with silver chloride nanoparticles (Ag@AgCl) produced by laccase from Trametes versicolor.
abstract: In the present study, semi-purified laccase from Trametes versicolor was applied for the synthesis of silver nanoparticles, and the properties of the produced nanoparticles were characterized. All of the analyses of the spectra indicated silver nanoparticle formation. A complete characterization of the silver nanoparticles showed that a complex of silver nanoparticles and silver ions was produced, with the majority of the particles having a Ag(2+) chemical structure. A hypothetical mechanistic scheme was proposed, suggesting that the main pathway that was used was the interaction of silver ions with the T1 site of laccase, producing silver nanoparticles with the concomitant inactivation of laccase activity and posterior complexing with silver ions.
authors.affiliation: Biological Chemistry Laboratory, Instituto Química, Universidade Estadual de Campinas, CP 6154, CEP 13083-970 Campinas, SP Brazil ; Laboratory on Nanostructures Synthesis and Biosystems Interactions (NanoBioss) (UNICAMP/SP), Campinas, SP Brazil.
authors.forename: Nelson
authors.initials: N
authors.lastname: Durán
authors.affiliation: Doctoral Program of Science of Natural Resources, Universidad de La Frontera, Temuco, Chile ; Environmental Biotechnology Center Science Nucleus BIOREN, Universidad de La Frontera, Temuco, Chile.
authors.forename: Raphael
authors.initials: R
authors.lastname: Cuevas
authors.affiliation: Institute of Biology, Universidade Estadual de Campinas, Campinas, SP Brazil.
authors.forename: Livia
authors.initials: L
authors.lastname: Cordi
authors.affiliation: Environmental Biotechnology Center Science Nucleus BIOREN, Universidad de La Frontera, Temuco, Chile ; Department of Chemical Engineering, Universidad de La Frontera, Temuco, Chile.
authors.forename: Olga
authors.initials: O
authors.lastname: Rubilar
authors.affiliation: Environmental Biotechnology Center Science Nucleus BIOREN, Universidad de La Frontera, Temuco, Chile ; Department of Chemical Engineering, Universidad de La Frontera, Temuco, Chile.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Diez
keywords: Laccase; Silver chloride nanoparticle; Silver nanoparticles; T. versicolor