title: Isolation, molecular and phylogenetic analysis of encephalomyocarditis virus strain GS01 in China.
abstract: Encephalomyocarditis virus (EMCV) is a small non-enveloped, single-stranded RNA virus. It can infect many host species and cause acute myocarditis and respiratory failure in piglets, reproductive failure in pregnant sows. Diseases caused by EMCV currently affect the swine industry worldwide. In this study, an EMCV strain was isolated from an aborted fetus in western China. It was identified by reverse-transcription polymerase chain reaction (RT-PCR) and genome sequencing. The subsequent results showed that the virus could produce a specific cytopathic effect on BHK-21 cells and could cause severe clinical symptoms and pathological changes in mice. Complete genome sequencing and multiple sequence alignment indicated that the GS01 strain was 79.9-99.9% identical with other isolates worldwide. Phylogenetic analysis showed that EMCV isolates fell into five clusters: lineage 1, 2, 3, 4, and 5 based on the nucleotide sequences of the entire ORF and VP3/VP1 junction, as well as 3D gene. GS01 isolate was grouped into lineage 1. The results of this study confirmed that an EMCV strain GS01 isolated from an aborted pig fetus in western China was fatal to mice and provided new epidemiologic data on EMCV in China.
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Ruofei
authors.initials: R
authors.lastname: Feng
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; School of Life Science and Bioengineering, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wei
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Li
authors.affiliation: The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Jingying
authors.initials: J
authors.lastname: Xie
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Li
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Zilin
authors.initials: Z
authors.lastname: Qiao
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Animal Cell Engineering & Technology Research Center of Gansu, Northwest University for Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China; The Key Bio-engineering and Technology Laboratory of SEAC, Northwest University For Nationalities, No. 1 Xibeixincun, Lanzhou 730030, PR China. Electronic address: mazhongren@xbmu.edu.cn.
authors.forename: Zhongren
authors.initials: Z
authors.lastname: Ma
mesh_terms: D000818:Animals; D018188:Cardiovirus Infections / Q000401:mortality / Q000662:veterinary / Q000821:virology*; D002460:Cell Line; D002681:China; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D010802:Phylogeny; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000821:virology*
keywords: EMCV; Isolation; Molecular characterization; Phylogenetic analysis