title: Effect of quantity of food residues on resistance to desiccation of food-related pathogens adhered to a stainless steel surface.
abstract: In order to study the effect of food residues on the survival of food-borne pathogens, Salmonella Typhimurium, Staphylococcus aureus, and Listeria monocytogenes were subjected to drying conditions in the presence of small amounts of food such as carrot juice, aqueous solution of nori, milk, and soy-milk. After drying for 2 h at room temperature in the absence of food residue, cell counts of S. Typhimurium, S. aureus, and L. monocytogenes decreased from 8 to 3, 6, and 5 log cfu/dish, respectively. Five milligrams of fresh carrot, 0.05 mg dried nori, and 100 nL milk or soy milk per 10 mm φ surface were sufficient to demonstrate a protective effect on the adhered pathogens, as confirmed by atomic force microscopy. Results from this study suggest that small sediments of food, not only protein rich but also carbohydrate rich, increase the resistance of surface-adherent bacteria to desiccation, rendering sanitization processes ineffective and encouraging cross contamination.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan. Electronic address: kuda@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Gensui
authors.initials: G
authors.lastname: Shibata
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo 108-8477, Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D003890:Desiccation; D005504:Food Analysis; D005511:Food Handling; D005516:Food Microbiology; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000502:physiology*; D050296:Microbial Viability; D013193:Stainless Steel / Q000032:analysis*
chemical_list: D013193:Stainless Steel
keywords: Food residue; Listeria monocytogenes; Salmonella Typhimurium; Staphylococcus aureus; Surface contamination