title: Inhibition of methyl-CoM Reductase from Methanobrevibacter ruminantium by 2-bromoethanesulfonate.
abstract: Cattle husbandry is a major contributor to atmospheric methane, which is considered as an important greenhouse gas. Moreover, the generation of methane in the intestine of domestic ruminants by methanogenic bacteria is a drag on feed efficacy. Studies on methanogenesis have typically implied model organisms that are, however, not relevant in the ruminant gut. This paper shows that methyl-CoM reductase catalyzing the final step of methanogenesis in Methanobrevibacter ruminantium, a major participant in methane production by cattle, is inhibited by 2-bromoethanesulfonate, a compound often used as a model in animal agriculture, with an apparent IC50 of 0.4 ± 0.04 μM.
authors.affiliation: Hamburg School of Food Science, Institute of Food Chemistry , Grindelallee 117, D-20146 Hamburg, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Gräwert
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Hohmann
authors.forename: Maik
authors.initials: M
authors.lastname: Kindermann
authors.forename: Stephane
authors.initials: S
authors.lastname: Duval
authors.forename: Adelbert
authors.initials: A
authors.lastname: Bacher
authors.forename: Markus
authors.initials: M
authors.lastname: Fischer
mesh_terms: D017738:Alkanesulfonic Acids / Q000737:chemistry*; D000818:Animals; D019843:Archaeal Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D007700:Kinetics; D008697:Methane / Q000096:biosynthesis; D041581:Methanobrevibacter / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012417:Rumen / Q000382:microbiology
chemical_list: D017738:Alkanesulfonic Acids; D019843:Archaeal Proteins; C018853:2-bromoethanesulfonic acid; D010088:Oxidoreductases; C021572:methyl coenzyme M reductase; D008697:Methane
keywords: Methanobrevibacter ruminantium; greenhouse gas; inhibition of methanogenesis; methyl-CoM reductase