title: Enhanced arachidonic acid production from Mortierella alpina combining atmospheric and room temperature plasma (ARTP) and diethyl sulfate treatments.
abstract: To obtain mutant strains with higher arachidonic acid (ARA) yields, the oleaginous fungus Mortierella alpina was mutated using atmospheric and room temperature plasma (ARTP) coupled with diethyl sulfate (DES). A visual compound filter operation was used in which a screening medium was supplemented with cerulenin, an inhibitor of fatty acid synthase (FAS), and triphenyltetrazolium chloride (TTC). The mutant strain D20 with an ARA production of 5.09 g/L, a 40.61% increase over the original strain (3.62 g/L), was isolated. The relative ARA content increased from 38.99% to 45.64% of total fatty acids. After optimizing fermentation conditions, the maximum ARA yield (6.82 g/L) for strain D20 was obtained in shake flasks. This work provides an appropriate strategy for obtaining high ARA-yield strains by conventional random mutation methods with an efficient screening assay.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China. Electronic address: mingchang@aliyun.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Yuanfa
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis*; D001272:Atmosphere; D061249:Batch Cell Culture Techniques; D018533:Biomass; D002108:Ceruletide / Q000494:pharmacology; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D020094:Mortierella / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D016296:Mutagenesis; D009154:Mutation; D009584:Nitrogen / Q000494:pharmacology; D058626:Plasma Gases / Q000494:pharmacology*; D013463:Sulfuric Acid Esters / Q000494:pharmacology*; D013696:Temperature
chemical_list: D058626:Plasma Gases; D013463:Sulfuric Acid Esters; D016718:Arachidonic Acid; D002108:Ceruletide; C011612:diethyl sulfate; D009584:Nitrogen
keywords: Arachidonic acid; Atmospheric and room temperature plasma (ARTP); Cerulenin; Mortierella alpina; Random mutagenesis