title: Analysis of human T-cell leukemia virus type 1 particles by using cryo-electron tomography.
abstract: The particle structure of human T-cell leukemia virus type 1 (HTLV-1) is poorly characterized. Here, we have used cryo-electron tomography to analyze HTLV-1 particle morphology. Particles produced from MT-2 cells were polymorphic, roughly spherical, and varied in size. Capsid cores, when present, were typically poorly defined polyhedral structures with at least one curved region contacting the inner face of the viral membrane. Most of the particles observed lacked a defined capsid core, which likely impacts HTLV-1 particle infectivity.
authors.affiliation: Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Cao
authors.affiliation: Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: José O
authors.initials: JO
authors.lastname: Maldonado
authors.affiliation: Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Iwen F
authors.initials: IF
authors.lastname: Grigsby
authors.affiliation: Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA mansky@umn.edu zhangwei@umn.edu.
authors.forename: Louis M
authors.initials: LM
authors.lastname: Mansky
authors.affiliation: Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA Department of Diagnostic and Biological Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA Characterization Facility, College of Science and Engineering, University of Minnesota, Minneapolis, Minnesota, USA mansky@umn.edu zhangwei@umn.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D020285:Cryoelectron Microscopy; D055032:Electron Microscope Tomography; D015368:Human T-lymphotropic virus 1 / Q000648:ultrastructure*; D006801:Humans; D014771:Virion / Q000648:ultrastructure*