title: A role for peroxidasin PXN-1 in aspects of C. elegans development.
abstract: The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Juyeon
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, West Bengal University of Technology, BF-142, Salt Lake City, Sector I, Kolkata 700-064, India.
authors.forename: Jaya
authors.initials: J
authors.lastname: Bandyopadhyay
authors.affiliation: Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju 220-710, Korea.
authors.forename: Jin Il
authors.initials: JI
authors.lastname: Lee
authors.affiliation: Department of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea.
authors.forename: Injeong
authors.initials: I
authors.lastname: Cho
authors.affiliation: Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
authors.forename: Daeho
authors.initials: D
authors.lastname: Park
authors.affiliation: Department of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea.
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Cho
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics / Q000254:growth & development*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism*; D005804:Genes, Lethal; D006358:Hot Temperature; D009154:Mutation; D009474:Neurons / Q000378:metabolism*; D009195:Peroxidase / Q000235:genetics* / Q000378:metabolism*; D054464:Peroxiredoxins / Q000235:genetics* / Q000378:metabolism*; D000097423:Peroxidasin
chemical_list: D029742:Caenorhabditis elegans Proteins; D016326:Extracellular Matrix Proteins; D009195:Peroxidase; D054464:Peroxiredoxins; D000097423:Peroxidasin; C000602044:PXN-1 protein, C elegans
keywords: C. elegans; heat stress; neuronal development; peroxidasins; pxn-1