title: Physiological and proteome studies of responses to heat stress during grain filling in contrasting wheat cultivars.
abstract: Experiments to explore physiological and biochemical differences of the effects of heat stress in ten wheat (Triticum aestivum L.) cultivars have been performed. Based on the response of photosynthesis rates, cell membrane lipid peroxide concentrations and grain yield to heat, six cultivars were clustered as heat-tolerant (cv. '579', cv. '810', cv. '1110', cv. Terice, cv. Taifun and cv. Vinjett) and four as heat-sensitive (cv. '490', cv. '633', cv. '1039' and cv. '1159'). Higher rates of photosynthetic carbon- and light-use were accompanied by lower damage to cell membranes in leaves of tolerant compared to sensitive cultivars under heat stress. The tolerant cv. '810' and the sensitive cv. '1039' were selected for further proteome analysis of leaves. Proteins related to photosynthesis, glycolysis, stress defence, heat shock and ATP production were differently expressed in leaves of the tolerant and sensitive cultivar under heat stress in relation to the corresponding control. The abundance of proteins related to signal transduction, heat shock, photosynthesis, and antioxidants increased, while the abundance of proteins related to nitrogen metabolism decreased in the tolerant cv. '810' under heat stress as compared to the control. Collectively, the results indicate that primarily changes in both the amount and activities of enzymes involved in photosynthesis and antioxidant activities in leaves contributed to higher heat tolerance in the cv. '810' compared to the heat sensitive cv. '1039'.
authors.affiliation: Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province, Nanjing Agricultural University, Nanjing 210095, China; Aarhus University, Faculty of Science and Technology, Institute of Agroecology, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark; Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Building 224, DK-2800 Kgs. Lyngby, Denmark. Electronic address: xiaowang@njau.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Sinop University, Sinop, Turkey.
authors.forename: Burcu Seckin
authors.initials: BS
authors.lastname: Dinler
authors.affiliation: Aarhus University, Faculty of Science and Technology, Institute of Agroecology, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark.
authors.forename: Marija
authors.initials: M
authors.lastname: Vignjevic
authors.affiliation: Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark, Building 224, DK-2800 Kgs. Lyngby, Denmark.
authors.forename: Susanne
authors.initials: S
authors.lastname: Jacobsen
authors.affiliation: Aarhus University, Faculty of Science and Technology, Institute of Agroecology, Research Centre Flakkebjerg, DK-4200 Slagelse, Denmark.
authors.forename: Bernd
authors.initials: B
authors.lastname: Wollenweber
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D016000:Cluster Analysis; D018869:Heat-Shock Response; D008054:Lipid Peroxides / Q000378:metabolism; D010788:Photosynthesis; D018526:Plant Transpiration; D020543:Proteome; D014908:Triticum / Q000378:metabolism / Q000502:physiology*
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D020543:Proteome; D002734:Chlorophyll
keywords: Antioxidant enzymes; Leaf proteome; Photosynthesis; Wheat