title: Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.
abstract: DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) is a key transcription factor for drought and heat stress tolerance in Arabidopsis thaliana. DREB2A induces the expression of dehydration- and heat stress-inducible genes under the corresponding stress conditions. Target gene selectivity is assumed to require stress-specific posttranslational regulation, but the mechanisms of this process are not yet understood. Here, we identified DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1), which was previously annotated as NUCLEAR FACTOR Y, SUBUNIT C10 (NF-YC10), as a DREB2A interactor, through a yeast two-hybrid screen. The overexpression of DPB3-1 in Arabidopsis enhanced the expression of a subset of heat stress-inducible DREB2A target genes but did not affect dehydration-inducible genes. Similarly, the depletion of DPB3-1 expression resulted in reduced expression of heat stress-inducible genes. Interaction and expression pattern analyses suggested the existence of a trimer comprising NF-YA2, NF-YB3, and DPB3-1 that could synergistically activate a promoter of the heat stress-inducible gene with DREB2A in protoplasts. These results suggest that DPB3-1 could form a transcriptional complex with NF-YA and NF-YB subunits and that the identified trimer enhances heat stress-inducible gene expression during heat stress responses in cooperation with DREB2A. We propose that the identified trimer contributes to the target gene selectivity of DREB2A under heat stress conditions.
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Sato
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Junya
authors.initials: J
authors.lastname: Mizoi
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba 305-8686, Japan.
authors.forename: Kyonosin
authors.initials: K
authors.lastname: Maruyama
authors.affiliation: Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences, Tsukuba 305-8686, Japan.
authors.forename: Feng
authors.initials: F
authors.lastname: Qin
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Osakabe
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Morimoto
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Ohori
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kusakabe
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan.
authors.forename: Maika
authors.initials: M
authors.lastname: Nagata
authors.affiliation: RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
authors.affiliation: Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan akys@mail.ecc.u-tokyo.ac.jp.
authors.forename: Kazuko
authors.identifier: http://orcid.org/0000-0002-0249-8258
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004257:DNA Polymerase II / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D018869:Heat-Shock Response / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; C114579:DREB2A protein, Arabidopsis; D014157:Transcription Factors; C000602079:DPB3-1 protein, Arabidopsis; D004257:DNA Polymerase II