title: Role of Dickeya dadantii 3937 chemoreceptors in the entry to Arabidopsis leaves through wounds.
abstract: Chemotaxis enables bacteria to move towards an optimal environment in response to chemical signals. In the case of plant-pathogenic bacteria, chemotaxis allows pathogens to explore the plant surface for potential entry sites with the ultimate aim to prosper inside plant tissues and to cause disease. Chemoreceptors, which constitute the sensory core of the chemotaxis system, are usually transmembrane proteins which change their conformation when sensing chemicals in the periplasm and transduce the signal through a kinase pathway to the flagellar motor. In the particular case of the soft-rot pathogen Dickeya dadantii 3937, jasmonic acid released in a plant wound has been found to be a strong chemoattractant which drives pathogen entry into the plant apoplast. In order to identify candidate chemoreceptors sensing wound-derived plant compounds, we carried out a bioinformatics search of candidate chemoreceptors in the genome of Dickeya dadantii 3937. The study of the chemotactic response to several compounds and the analysis of the entry process to Arabidopsis leaves of 10 selected mutants in chemoreceptors allowed us to determine the implications of at least two of them (ABF-0020167 and ABF-0046680) in the chemotaxis-driven entry process through plant wounds. Our data suggest that ABF-0020167 and ABF-0046680 may be candidate receptors of jasmonic acid and xylose, respectively.
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.|Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Río-Álvarez
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.|Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Muñoz-Gómez
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.|Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: Mariela
authors.initials: M
authors.lastname: Navas-Vásquez
authors.affiliation: Área de Genética, Facultad de Ciencias, Instituto de Hortofruticultura Subtropical y Mediterránea 'La Mayora' (IHSM-UMA-CSIC), Universidad de Málaga, E-29071, Málaga, Spain.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Martínez-García
authors.affiliation: Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Antúnez-Lamas
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.|Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Rodríguez-Palenzuela
authors.affiliation: Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Parque Científico y Tecnológico de la UPM, Universidad Politécnica de Madrid, Campus de Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain.|Departamento de Biotecnología, Escuela Técnica Superior de Ingenieros Agrónomos, UPM, Avda, Complutense S/N, 28040, Madrid, Spain.
authors.forename: Emilia
authors.initials: E
authors.lastname: López-Solanilla
mesh_terms: D017360:Arabidopsis / Q000382:microbiology*; D004755:Enterobacteriaceae / Q000378:metabolism*; D018515:Plant Leaves / Q000382:microbiology*; D010940:Plant Proteins / Q000378:metabolism
chemical_list: D010940:Plant Proteins
keywords: Dickeya dadantii 3937; chemoreceptors; entry; jasmonic acid; xylose