title: Genetic differentiation among 6 populations of red deer (Cervus elaphus L.) in Poland based on microsatellite DNA polymorphism.
abstract: Recently, there has been considerable interest in genetic differentiation in the Cervidae family. A common tool used to determine genetic variation in different species, breeds and populations is DNA analysis, which allows for direct determination of the differences and changes within a group of animals. Because the analysis of microsatellite polymorphism in different Cervidae populations revealed considerable genetic variability in individual populations, it was important to test a set of markers in animals from these populations.The study was performed with muscle tissue and blood samples collected from a total of 793 red deer. Six groups (subpopulations) of red deer were defined according to region: Masurian (330 animals), Bieszczady (194 animals), Małopolska (80 animals), Sudety (76 animals), Lower Silesian (62 animals) and Lubusz (51 animals). The analysis involved 12 STR markers (BM1818, OarAE129, OarFCB5, OarFCB304, RM188, RT 1, RT 13, T26, T156, T193, T501, TGLA53), for which conditions for simultaneous amplification were established.Based on this study, it is concluded that the chosen set of 12 microsatellite markers could be used to evaluate the genetic structure and to monitor changes in Poland's red deer population.
authors.affiliation: National Research Institute of Animal Production Department of Animal Cytogenetics and Molecular Genetics Krakowska 1 32-083 Balice Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Radko
authors.affiliation: University of Warmia and Mazury in Olsztyn Department of Fur-bearing Animal Breeding and Game Management Oczapowskiego 5 10-719 Olsztyn Poland.
authors.forename: D
authors.initials: D
authors.lastname: Zalewski
authors.affiliation: National Research Institute of Animal Production Department of Animal Cytogenetics and Molecular Genetics Krakowska 1 32-083 Balice Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Rubiś
authors.affiliation: National Research Institute of Animal Production Department of Animal Cytogenetics and Molecular Genetics Krakowska 1 32-083 Balice Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szumiec
mesh_terms: D000818:Animals; D003670:Deer / Q000235:genetics*; D056426:Genetic Loci; D005828:Genetics, Population / Q000379:methods*; D005838:Genotype; D018895:Microsatellite Repeats; D011044:Poland; D011110:Polymorphism, Genetic; D011156:Population Density; D011157:Population Dynamics
keywords: Cervidae; STR population studies; microsatellite DNA sequences