title: Crystallization and preliminary X-ray characterization of the full-length bacteriophytochrome from the plant pathogen Xanthomonas campestris pv. campestris.
abstract: Phytochromes give rise to the largest photosensor family known to date. However, they are underrepresented in the Protein Data Bank. Plant, cyanobacterial, fungal and bacterial phytochromes share a canonical architecture consisting of an N-terminal photosensory module (PAS2-GAF-PHY domains) and a C-terminal variable output module. The bacterium Xanthomonas campestris pv. campestris, a worldwide agricultural pathogen, codes for a single bacteriophytochrome (XccBphP) that has this canonical architecture, bearing a C-terminal PAS9 domain as the output module. Full-length XccBphP was cloned, expressed and purified to homogeneity by nickel-NTA affinity and size-exclusion chromatography and was then crystallized at room temperature bound to its cofactor biliverdin. A complete native X-ray diffraction data set was collected to a maximum resolution of 3.25 Å. The crystals belonged to space group P43212, with unit-cell parameters a = b = 103.94, c = 344.57 Å and a dimer in the asymmetric unit. Refinement is underway after solving the structure by molecular replacement.
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Klinke
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Lisandro H
authors.initials: LH
authors.lastname: Otero
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Jimena
authors.initials: J
authors.lastname: Rinaldi
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Santiago
authors.initials: S
authors.lastname: Sosa
authors.affiliation: Synchrotron SOLEIL, L'Orme des Merisiers, Saint-Aubin BP 48, 91192 Gif-sur-Yvette CEDEX, France.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: Guimarães
authors.affiliation: Synchrotron SOLEIL, L'Orme des Merisiers, Saint-Aubin BP 48, 91192 Gif-sur-Yvette CEDEX, France.
authors.forename: William E
authors.initials: WE
authors.lastname: Shepard
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Fernando A
authors.initials: FA
authors.lastname: Goldbaum
authors.affiliation: Fundación Instituto Leloir, IIBBA-CONICET, Avenida Patricias Argentinas 435, Buenos Aires, C1405BWE Buenos Aires, Argentina.
authors.forename: Hernán R
authors.initials: HR
authors.lastname: Bonomi
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D010834:Phytochrome / Q000737:chemistry*; D016959:Xanthomonas campestris / Q000737:chemistry*
chemical_list: D010834:Phytochrome
keywords: Xanthomonas campestris pv. campestris; bacteriophytochrome; biliverdin; light-signalling pathway; photoreceptor; photosensor