title: Gene expression profiling of coelomic cells and discovery of immune-related genes in the earthworm, Eisenia andrei, using expressed sequence tags.
abstract: The coelomic cells of the earthworm consist of leukocytes, chlorogocytes, and coelomocytes, which play an important role in innate immunity reactions. To gain insight into the expression profiles of coelomic cells of the earthworm, Eisenia andrei, we analyzed 1151 expressed sequence tags (ESTs) derived from the cDNA library of the coelomic cells. Among the 1151 ESTs analyzed, 493 ESTs (42.8%) showed a significant similarity to known genes and represented 164 unique genes, of which 93 ESTs were singletons and 71 ESTs manifested as two or more ESTs. From the 164 unique genes sequenced, we found 24 immune-related and cell defense genes. Furthermore, real-time PCR analysis showed that levels of lysenin-related proteins mRNA in coelomic cells of E. andrei were upregulated after the injection of Bacillus subtilis bacteria. This EST data-set would provide a valuable resource for future researches of earthworm immune system.
authors.affiliation: a Department of Life Sciences , Chung-Ang University , Seoul , Republic of Korea.
authors.forename: Eun Sik
authors.initials: ES
authors.lastname: Tak
authors.forename: Sung-Jin
authors.initials: SJ
authors.lastname: Cho
authors.forename: Soon Cheol
authors.initials: SC
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004926:Escherichia coli / Q000502:physiology; D020224:Expressed Sequence Tags / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000166:cytology / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014118:Toxins, Biological / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D014118:Toxins, Biological; C107040:lysenin
keywords: coelomic cells; earthworm; expressed sequence tags; innate immunity; lysenin-related proteins