title: Enzymatic glucose sensor based on Au nanoparticle and plant-like ZnO film modified electrode.
abstract: A novel electrochemical glucose sensor was developed by employing a composite film of plant-like Zinc oxide (ZnO) and chitosan stabilized spherical gold nanoparticles (AuNPs) on which Glucose oxidaze (GOx) was immobilized. The ZnO was deposited on an indium tin oxide (ITO) coated glass and the AuNPs of average diameter of 23 nm were loaded on ZnO as the second layer. The prepared ITO/ZnO/AuNPs/GOx bioelectrode exhibited a low value of Michaelis-Menten constant of 1.70 mM indicating a good bio-matrix for GOx. The studies of electrochemical properties of the electrode using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) showed that, the presence of AuNPs provides significant enhancement of the electron transfer rate during redox reactions. The linear sweep voltammetry (LSV) shows that the ITO/ZnO/AuNPs/GOx based sensor has a high sensitivity of 3.12 μA·mM(-1)·cm(-2) in the range of 50 mg/dL to 400 mg/dL glucose concentration. The results show promising application of the gold nanoparticle modified plant-like ZnO composite bioelectrode for electrochemical sensing of glucose.
authors.affiliation: Nanostructured Materials Research Laboratory, Department of Materials Science and Engineering, University of Utah, Salt Lake City, UT 84112, United States.
authors.forename: Kun
authors.initials: K
authors.lastname: Tian
authors.affiliation: Nanostructured Materials Research Laboratory, Department of Materials Science and Engineering, University of Utah, Salt Lake City, UT 84112, United States; Department of Chemistry, Government College for Women, Thiruvananthapuram, Kerala 695014, India.
authors.forename: Saji
authors.initials: S
authors.lastname: Alex
authors.affiliation: Nanostructured Materials Research Laboratory, Department of Materials Science and Engineering, University of Utah, Salt Lake City, UT 84112, United States.
authors.forename: Gene
authors.initials: G
authors.lastname: Siegel
authors.affiliation: Nanostructured Materials Research Laboratory, Department of Materials Science and Engineering, University of Utah, Salt Lake City, UT 84112, United States. Electronic address: tiwari@eng.utah.edu.
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Tiwari
mesh_terms: D058266:Dielectric Spectroscopy; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D006046:Gold; D005947:Glucose; D015034:Zinc Oxide
keywords: AuNPs; Electrochemical properties; Glucose sensors; ZnO