title: Characterization of alcohol dehydrogenase from Kangiella koreensis and its application to production of all-trans-retinol.
abstract: A recombinant alcohol dehydrogenase (ADH) from Kangiella koreensis was purified as a 40 kDa dimer with a specific activity of 21.3 nmol min(-1) mg(-1), a K m of 1.8 μM, and a k cat of 1.7 min(-1) for all-trans-retinal using NADH as cofactor. The enzyme showed activity for all-trans-retinol using NAD (+) as a cofactor. The reaction conditions for all-trans-retinol production were optimal at pH 6.5 and 60 °C, 2 g enzyme l(-1), and 2,200 mg all-trans-retinal l(-1) in the presence of 5% (v/v) methanol, 1% (w/v) hydroquinone, and 10 mM NADH. Under optimized conditions, the ADH produced 600 mg all-trans-retinol l(-1) after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg l(-1) h(-1). This is the first report of the characterization of a bacterial ADH for all-trans-retinal and the biotechnological production of all-trans-retinol using ADH.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Seung-Hye
authors.initials: SH
authors.lastname: Hong
authors.forename: Ho-Phuong-Thuy
authors.initials: HP
authors.lastname: Ngo
authors.forename: Lin-Woo
authors.initials: LW
authors.lastname: Kang
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D053521:Alcanivoraceae / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D003067:Coenzymes / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006873:Hydroquinones / Q000378:metabolism; D007700:Kinetics; D000432:Methanol / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D010802:Phylogeny; D055503:Protein Multimerization; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013696:Temperature; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D003067:Coenzymes; D006873:Hydroquinones; D011994:Recombinant Proteins; D009243:NAD; D014801:Vitamin A; D000426:Alcohol Dehydrogenase; C031927:hydroquinone; D000432:Methanol