title: High loading MnO2 nanowires on graphene paper: facile electrochemical synthesis and use as flexible electrode for tracking hydrogen peroxide secretion in live cells.
abstract: Recent progress in flexible and lightweight electrochemical sensor systems requires the development of paper-like electrode materials. Here, we report a facile and green synthesis of a new type of MnO2 nanowires-graphene nanohybrid paper by one-step electrochemical method. This strategy demonstrates a collection of unique features including the effective electrochemical reduction of graphene oxide (GO) paper and the high loading of MnO2 nanowires on electrochemical reduced GO (ERGO) paper. When used as flexible electrode for nonenzymatic detection of hydrogen peroxide (H2O2), MnO2-ERGO paper exhibits high electrocatalytic activity toward the redox of H2O2 as well as excellent stability, selectivity and reproducibility. The amperometric responses are linearly proportional to H2O2 concentration in the range 0.1-45.4 mM, with a detection limit of 10 μM (S/N=3) and detection sensitivity of 59.0 μA cm(-2) mM(-1). These outstanding sensing performances enable the practical application of MnO2-ERGO paper electrode for the real-time tracking H2O2 secretion by live cells macrophages. Therefore, the proposed graphene-based nanohybrid paper electrode with intrinsic flexibility, tailorable shapes and adjustable properties can contribute to the full realization of high-performance flexible electrode material used in point-of-care testing devices and portable instruments for in-vivo clinical diagnostics and on-site environmental monitoring.
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Xi
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Fu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China. Electronic address: liuhf@hust.edu.cn.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Chemical Engineering, Hubei Key Laboratory of Material Chemistry and Service Failure, Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, Huazhong University of Science & Technology, Wuhan 430074, PR China. Electronic address: xiaofei@hust.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002460:Cell Line; D055664:Electrochemical Techniques; D004566:Electrodes; D005292:Ferricyanides / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D017895:Manganese Compounds / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D010209:Paper
chemical_list: D005292:Ferricyanides; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D006108:Graphite; D006861:Hydrogen Peroxide; C028033:potassium ferricyanide
keywords: Flexible electrode; Graphene-based nanohybrid paper; Live cell tracking; Nonenzymatic hydrogen peroxide sensing; One-step electrochemical synthesis