title: In vitro toxicity of iron oxide nanoparticle: oxidative damages on Hep G2 cells.
abstract: During the past years many studies have been done highlighting the great need for a more thorough understanding of cell-iron oxide nanoparticle interactions. To improve our knowledge in this field, there is a great need for standardized protocols that would allow to comparing the cytotoxic potential of any Fe2O3-NP type with previously studied particles. Several approaches are reported that several parameters which are of great importance for Fe2O3 nanoparticle induced toxicity. Nanoparticles because of their very small size can pass through the cell membrane and can make oxidative damage in all parts of the cells such as mitochondria, membrane, DNA due to high surface area. This study focuses on acute cytotoxicity of reactive oxygen species and DNA damaging effects of mentioned nanoparticles. Results showed increase of the oxidative damage leads cells to the apoptosis, therefore reduced cell viability. It is interesting that all of the results are concentration and time dependent.
authors.affiliation: Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Sadeghi
authors.affiliation: Department of Odontology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Farzeen
authors.initials: F
authors.lastname: Tanwir
authors.affiliation: Department of Physiology, Payam Noor University of Iran, Iran. Electronic address: v.yoosefi@gmail.com.
authors.forename: Vahid
authors.initials: V
authors.lastname: Yousefi Babadi
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage; D005290:Ferric Compounds / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D053758:Nanoparticles / Q000633:toxicity*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D017382:Reactive Oxygen Species; C000499:ferric oxide; D005978:Glutathione
keywords: Anti oxidative barrier; Apoptosis pathway; DNA fragmentation; Iron oxide nanoparticle; Reactive oxygen species