title: A novel fully automated on-line coupled liquid chromatography-gas chromatography technique used for the determination of organochlorine pesticide residues in tobacco and tobacco products.
abstract: In this study, a novel fully automated on-line coupled liquid chromatography-gas chromatography (LC-GC) technique was reported and applied for the determination of organochlorine pesticide residues (OCPs) in tobacco and tobacco products. Using a switching valve to isolate the capillary pre-column and the analytical column during the solvent evaporation period, the LC solvent can be completely removed and prevented from reaching the GC column and the detector. The established method was used to determinate the OCPs in tobacco samples. By using Florisil SPE column and employing GPC technique, polarity impurities and large molecule impurities were removed. A dynamic range 1-100ng/mL was achieved with detection limits from 1.5 to 3.3μg/kg. The method exhibited good repeatability and recoveries. This technology may provide an alternative way for trace analysis of complex samples.
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qi
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fei
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China. Electronic address: shayf@sh.tobacco.com.cn.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Sha
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China.
authors.forename: Leijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China.
authors.forename: Da
authors.initials: D
authors.lastname: Wu
authors.affiliation: Technical Center of Shanghai Tobacco Group Co. Ltd., Shanghai 200082, China. Electronic address: liubz@sh.tobacco.com.cn.
authors.forename: Baizhan
authors.initials: B
authors.lastname: Liu
mesh_terms: D057205:Automation, Laboratory; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006843:Hydrocarbons, Chlorinated / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D006843:Hydrocarbons, Chlorinated; D010573:Pesticide Residues; D012997:Solvents
keywords: Electron capture detector; On-column injection; On-line coupled liquid chromatography–gas chromatography; Organochlorine pesticide residues; Tobacco