title: Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation.
abstract: Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products. However, it has not been possible to control regio- and stereoselectivity in a general manner, which is necessary for wide (industrial) applications. Especially directed evolution as a Darwinian approach to protein engineering has changed the situation for the better during the last 3-4 years leading to extensive progress, which is summarized and analysed in this Feature Article.
authors.affiliation: GlaxoSmithKline R&D Ltd, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, UK.
authors.forename: Gheorghe-Doru
authors.initials: GD
authors.lastname: Roiban
authors.forename: Manfred T
authors.initials: MT
authors.lastname: Reetz
mesh_terms: D055162:Biocatalysis; D020134:Catalytic Domain; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019143:Evolution, Molecular; D006900:Hydroxylation; D056004:Molecular Dynamics Simulation; D009930:Organic Chemicals / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011110:Polymorphism, Genetic; D017434:Protein Structure, Tertiary; D013237:Stereoisomerism; D013739:Testosterone / Q000737:chemistry / Q000378:metabolism
chemical_list: D009930:Organic Chemicals; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System