title: Myo10 is required for neurogenic cell adhesion and migration.
abstract: Myosin X (Myo10), an untraditional member of myosin superfamily, is characterized as an actin-based molecular motor, which plays a critical role in diverse cellular motile events. Previous research by our group has found that Myo10 influenced neuronal radial migration in developing neocortex, but the underlying mechanism is still largely unknown. In this study, we found that knockdown of endogenous Myo10 in a normal gonadotropin-releasing hormone (GnRH) neuronal cell line transfected with the large T antigen (NLT) induced the impairment of cell motility and orientation. In the wound healing assay, with the Golgi complex staining to display cell polarity, Myo10 knockdown cells were randomly oriented compared to the control. Furthermore, suppressing the expression of Myo10 decreased the ability of cell-matrix adhesion. N-cadherin, a calcium-dependent classical cell adhesion molecule, rescued the migration deficiency caused by Myo10 knockdown in cell aggregates and collagen gel assay. These results suggest that Myo10 is required for neurogenic cell migration through N-cadherin mediated cell adhesion.
authors.affiliation: Key laboratory of Molecular Epigenetics of Ministry of Education, Institute of Cytology and Genetics, Northeast Normal University, No. 5268, Renmin Street, Changchun, Jilin, 130024, People's Republic of China.
authors.forename: Huali
authors.initials: H
authors.lastname: Yu
authors.forename: Mingming
authors.initials: M
authors.lastname: Lai
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Guo
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Lan
authors.forename: Xingzhi
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D055785:Gene Knockdown Techniques; D051379:Mice; D009218:Myosins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000502:physiology
chemical_list: D015820:Cadherins; C489083:Cdh2 protein, mouse; C409064:Myo10 protein, mouse; D009218:Myosins