title: Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms.
abstract: Interleukin (IL)-10 is an important immunoregulatory cytokine that mediates its effects via a transmembrane receptor complex consisting of two different chains, IL-10R1 and IL-10R2. While IL-10R2 is ubiquitously expressed and does not bind IL-10 primarily, the expression of IL-10R1 determines cellular responsiveness. However, the current knowledge about the expression and regulation of IL-10R1 is still limited. Here we analyzed the expression of IL-10R1 on monocytic cells and demonstrated that human blood monocytes carried about 720 IL-10-binding sites on their surface. Compared with lymphocytes and various tissue cells and tissues, blood monocytes expressed the highest IL-10R1 levels. The in vitro differentiation of these cells into macrophages provoked a further increase of IL-10R1 surface expression. In contrast, their differentiation into myeloid dendritic cells (mDCs) resulted in reduced surface IL-10R1 levels. The different IL-10R1 levels expressed by monocyte-derived antigen-presenting cell populations were reflected in their different responsiveness toward IL-10. Importantly, also in vivo developed immature macrophages and mDCs showed different IL-10 sensitivity. These data suggest that, compared with monocytes and macrophages, mDCs partially escape from IL-10's inhibitory mechanisms by downregulating IL-10R1.
authors.forename: S
authors.initials: S
authors.lastname: von Haehling
authors.affiliation: Institute of Medical Immunology, University Hospital Charité, Berlin, Germany.
authors.forename: S H
authors.initials: SH
authors.lastname: von Lanzenauer
authors.affiliation: 1] Interdisciplinary Group of Molecular Immunopathology, Dermatology/Medical Immunology, University Hospital Charité, Berlin, Germany [2] Research Center Immunosciences, University Hospital Charité, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wolk
authors.affiliation: Institute of Medical Immunology, University Hospital Charité, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Höflich
authors.affiliation: Interdisciplinary Group of Molecular Immunopathology, Dermatology/Medical Immunology, University Hospital Charité, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Kunz
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: B H
authors.initials: BH
authors.lastname: Grünberg
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: W-D
authors.initials: WD
authors.lastname: Döcke
authors.affiliation: Institute of Medical Immunology, University Hospital Charité, Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Reineke
authors.affiliation: Bayer Pharma AG, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Asadullah
authors.affiliation: Department of Dermatology and Allergy, University Hospital Charité, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Sterry
authors.affiliation: Institute of Medical Immunology, University Hospital Charité, Berlin, Germany.
authors.forename: H-D
authors.initials: HD
authors.lastname: Volk
authors.affiliation: 1] Interdisciplinary Group of Molecular Immunopathology, Dermatology/Medical Immunology, University Hospital Charité, Berlin, Germany [2] Research Center Immunosciences, University Hospital Charité, Berlin, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Sabat
mesh_terms: D003713:Dendritic Cells / Q000276:immunology; D005347:Fibroblasts / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology*; D053709:Interleukin-10 Receptor alpha Subunit / Q000235:genetics / Q000276:immunology*; D015603:Keratinocytes / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology
chemical_list: C508609:IL10 protein, human; D053709:Interleukin-10 Receptor alpha Subunit; D016753:Interleukin-10