title: Exosome from chaperone-rich cell lysates-loaded dendritic cells produced by CELLine 1000 culture system exhibits potent immune activity.
abstract: Dendritic cells (DCs) pulsed with exosomes can stimulate efficient cytotoxic T-lymphocyte responses and anti-tumor immunity. However, the quantity of DC-derived exosomes (DCex) obtained from various culture systems is very low, which is a significant practical issue hampering progress in this research area and needs to be addressed. Gliomas were particularly aggressive, with high morbidity and mortality, indicating that this is a form of incurable highly malignant tumor of the brain with poor prognosis. In the present study, we demonstrate that the CELLine 1000 culture system can dramatically increase the production of DCex. The morphology, phenotype and immune molecules of these DCex were found to be identical to those using traditional methods. Our researches supply a cost-effective, useful method for significantly increasing the quantity of exosomes. In addition, GL261 glioma cells were chosen to separate chaperone-rich cell lysates (CRCL). The results indicate that CRCL-GL261 cell lysates can trigger the most intense expression of immune molecules on DCex or DCs, which has important implications for the research into tumor treatment and diagnosis.
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: ningbuxa@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Bu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Guilian
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ma
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Nina
authors.initials: N
authors.lastname: Zhai
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003470:Culture Media; D003713:Dendritic Cells / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D005910:Glioma / Q000276:immunology* / Q000473:pathology; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D016253:Microscopy, Immunoelectron; D018832:Molecular Chaperones / Q000276:immunology*; D010641:Phenotype; D011379:Prognosis; D013601:T-Lymphocytes; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology
chemical_list: D000970:Antineoplastic Agents; D003470:Culture Media; D018832:Molecular Chaperones
keywords: Chaperone-rich cell lysates (CRCL); DC-derived exosomes (DCex); Dendritic cells (DCs); Glioma