title: Nano-characterization of two closely related melanoma cell lines with different metastatic potential.
abstract: Cutaneous malignant melanoma is one of the most lethal types of skin cancer. Its progression passes through several steps, leading to the appearance of a new population of cells with aggressive biological potential. Here, we focused on the nano-characterization of two different melanoma cell lines with similar morphological appearance but different metastatic potential, namely, WM115 from vertical growth phase (VGP) and WM266-4 derived from metastasis to skin. The first cell line represents cells that progressed to the VGP, while the WM266-4 cell line denotes cells from the metastasis to skin. Exploring with a combination of atomic force and fluorescence microscopes, our goal was to identify cell surface characteristics in both cell lines that may determine differences in the cellular nano-mechanical properties. Cell elasticity was found to be affected by the presence of F-actin-based flexible ridges, rich in F-actin co-localized with β1 integrins in the studied cell lines. These results point out how progressive changes in the surface structure of melanoma cells can affect their bionanomechanical properties.
authors.affiliation: The Henryk Niewodniczański Institute of Nuclear Physics, Polish Academy of Sciences, Radzikowskiego 152, 31-342, Kraków, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Gostek
authors.forename: Szymon
authors.initials: S
authors.lastname: Prauzner-Bechcicki
authors.forename: Benedikt
authors.initials: B
authors.lastname: Nimmervoll
authors.forename: Katrin
authors.initials: K
authors.lastname: Mayr
authors.forename: Joanna
authors.initials: J
authors.lastname: Pabijan
authors.forename: Peter
authors.initials: P
authors.lastname: Hinterdorfer
authors.forename: Lilia A
authors.initials: LA
authors.lastname: Chtcheglova
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Lekka
mesh_terms: D000199:Actins / Q000378:metabolism; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D004548:Elasticity; D006801:Humans; D039641:Integrin beta Chains / Q000378:metabolism; D008545:Melanoma / Q000378:metabolism / Q000648:ultrastructure*
chemical_list: D000199:Actins; D039641:Integrin beta Chains