title: Comparative secretomic and N-glycoproteomic profiling in human MCF-7 breast cancer and HMEpC normal epithelial cell lines using a gel-based strategy.
abstract: BACKGROUND
  Concurrent study of secretomic and glycoproteomic profiles in cancer cell lines represents an excellent approach for investigating cancer progression and identifying novel biomarker candidates. In this study, we performed a comparative secretomic and N-glycoprotein profiling from the secretions of normal human mammary epithelial cells (HMEpC) and the MCF-7 human breast cancer cell line.
  
  
  METHODS
  We analyzed these cell lines using a combined methodology involving glycan-binding lectins and two-dimensional electrophoresis and identified several differentially secreted factors, including osteonectin and haptoglobin.
  
  
  RESULTS
  Notably, comparative analyses also revealed that MCF-7 cells produced differentially N-glycosylated forms of haptoglobin.
  
  
  CONCLUSIONS
  The present data suggested that osteonectin and haptoglobin might have potential to be served as potential biomarkers for breast cancer. However, further investigation is needed to validate the findings.
authors.affiliation: Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Penang, 11800 Malaysia.
authors.forename: Aik-Aun
authors.initials: AA
authors.lastname: Tan
authors.affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, 50603 Malaysia.
authors.forename: Alan Kang-Wai
authors.initials: AK
authors.lastname: Mu
authors.affiliation: Department of Pharmacology, Faculty of Medicine Building, University of Malaya, Kuala Lumpur, 50603 Malaysia.
authors.forename: Lik-Voon
authors.initials: LV
authors.lastname: Kiew
authors.affiliation: Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, 50603 Malaysia ; Oral Cancer Research and Coordinating Centre, Faculty of Dentistry, University of Malaya, Kuala Lumpur, 50603 Malaysia.
authors.forename: Yeng
authors.initials: Y
authors.lastname: Chen
keywords: Concanavalin A lectin; Glycoproteomic; N-glycoprotein; Secretomic; Two dimensional electrophoresis