title: Genetic diversity and gene structure of mitochondrial region of Anopheles minimus (Diptera: Culicidae) - major malaria vector of North east India.
abstract: OBJECTIVE
  To depict mitochondrial genetic variation for the first time among Anopheles minimus (An.minimus) (Diptera: Culicidae) species from two malaria endemic states of NE India.
  
  
  METHODS
  Phylogeographic analysis was carried at 9 out of 12 sites of An.minimus confirmed malaria endemic places.
  
  
  RESULTS
  All sequences were Adenine-Thymine rich regions. Transitions were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 case non synonymous mutation was observed. Three distinct clusters of haplotypes were generated. Haplotype diversity and low nucleotide diversity were studied. Overall negative values obtained from Tajima's D test and Fu'sFS test indicate a recent genetic population expansion. Network analysis has explained sequence diversity that was also shown by mutations in 6 sequences.
  
  
  CONCLUSIONS
  High genetic diversity observed within the populations of An.minimus species has several possible implications for vector control in the region.
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India. Electronic address: duttaprafulla@yahoo.com.
authors.forename: Prafulla
authors.initials: P
authors.lastname: Dutta
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Siraj Ahmed
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Rashmee
authors.initials: R
authors.lastname: Topno
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Pritom
authors.initials: P
authors.lastname: Chowdhury
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Mayuri
authors.initials: M
authors.lastname: Baishya
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Purvita
authors.initials: P
authors.lastname: Chowdhury
authors.affiliation: Regional Medical Research Centre, ICMR (NE Region), Dibrugarh, Assam, India.
authors.forename: Jagadish
authors.initials: J
authors.lastname: Mahanta
keywords: Anopheles minimus; Cytochrome oxidase II; Haplotype; Mutation; Substitution