title: The effects of recombinant human epithelialgrowth factor and protein-free calf blood extract for recovery of corneal mechanical epithelial defects healing and neovascularization.
abstract: OBJECTIVE
  This study was undertaken to investigate the effects of recombinant human epidermal growth factor (rhEGF) and protein-free calf blood extract on corneal wound healing and neovascularization.
  
  
  METHODS
  An rabbit central corneal wound and neovascularization model was established in rabbits. One eye of each group was chosen randomly for topical administration of protein-free calf blood extract, rhEGF, or NS (physiological saline), and variability in the area of corneal epithelial wound healing and neovascularization was observed.
  
  
  RESULTS
  On days 1 and 2, the healing rate of corneal epithelium was various among the protein-free calf blood extract group, rhEGF group and NS group (F=6.475, p=0.012). The healing rate of corneal epithelium in the rhEGF group was better than the protein-free calf blood extract group (p=0.004) and NS group (p=0.041) on day 1. The corneal neovascularization area in the protein-free calf blood extract group was less than that of rhEGF group (p=0.04) and NS group (p=0.008) on day 18.
  
  
  CONCLUSIONS
  rhEGF had better promotive effect on corneal epithelial wound healing than the protein-free calf blood extract in the advanced phase (within 2 days). Both rhEGF and protein-free calf blood extract were not found to promote.
authors.affiliation: Department of Ophthalmology, ZhuJiang Hospital of Southern Medical University, Guangzhou, PR China. wuwei200414952@126.com.
authors.forename: W
authors.initials: W
authors.lastname: Wu
authors.forename: L N
authors.initials: LN
authors.lastname: Zeng
authors.forename: Y Y
authors.initials: YY
authors.lastname: Peng
authors.forename: X H
authors.initials: XH
authors.lastname: Lu
authors.forename: C Y
authors.initials: CY
authors.lastname: Li
authors.forename: Z C
authors.initials: ZC
authors.lastname: Wang
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002417:Cattle; D004815:Epidermal Growth Factor / Q000008:administration & dosage*; D019573:Epithelium, Corneal / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000473:pathology; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage; D020127:Recovery of Function / Q000187:drug effects* / Q000502:physiology; D014020:Tissue Extracts / Q000008:administration & dosage*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D011994:Recombinant Proteins; D014020:Tissue Extracts; D004815:Epidermal Growth Factor