title: Dandelion PPO-1/PPO-2 domain-swaps: the C-terminal domain modulates the pH optimum and the linker affects SDS-mediated activation and stability.
abstract: Plant polyphenol oxidases (PPOs) have a conserved three-domain structure: (i) the N-terminal domain (containing the active site) is connected via (ii) a linker to (iii) the C-terminal domain. The latter covers the active site, thereby maintaining the enzyme in a latent state. Activation can be achieved with SDS but little is known about the mechanism. We prepared domain-swap variants of dandelion PPO-1 and PPO-2 to test the specific functions of individual domains and their impact on enzyme characteristics. Our experiments revealed that the C-terminal domain modulates the pH optimum curve and has a strong influence on the optimal pH value. The linker determines the SDS concentration required for full activation. It also influences the SDS concentration required for half maximal activation (kSDS) and the stability of the enzyme during prolonged incubation in buffers containing SDS, but the N-terminal domain has the strongest effect on these parameters. The N-terminal domain also determines the IC50 of SDS and the stability in buffers containing or lacking SDS. We propose that the linker and C-terminal domain fine-tune the activation of plant PPOs. The C-terminal domain adjusts the pH optimum and the linker probably contains an SDS-binding/interaction site that influences inactivation and determines the SDS concentration required for activation. For the first time, we have determined the influence of the three PPO domains on enzyme activation and stability providing insight into the regulation and activation mechanisms of type-3 copper proteins in general.
authors.affiliation: Institute of Plant Biology and Biotechnology, Westphalian Wilhelm's-University of Münster, Schlossplatz 8, 48143 Münster, Germany. Electronic address: christine.leufken@uni-muenster.de.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Leufken
authors.affiliation: Institute of Plant Biology and Biotechnology, Westphalian Wilhelm's-University of Münster, Schlossplatz 8, 48143 Münster, Germany. Electronic address: moersch@uni-muenster.de.
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Moerschbacher
authors.affiliation: Institute of Plant Biology and Biotechnology, Westphalian Wilhelm's-University of Münster, Schlossplatz 8, 48143 Münster, Germany. Electronic address: m.dirks-hofmeister@uni-muenster.de.
authors.forename: Mareike E
authors.initials: ME
authors.lastname: Dirks-Hofmeister
mesh_terms: D001665:Binding Sites; D004156:Catechol Oxidase / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017434:Protein Structure, Tertiary; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D031205:Taraxacum / Q000737:chemistry / Q000201:enzymology*
chemical_list: D012967:Sodium Dodecyl Sulfate; D004156:Catechol Oxidase
keywords: Activation mechanism; Domain-swap; Polyphenol oxidase; SDS activation; SDS stability; Three-domain structure