title: Influence of Cu content on the cell biocompatibility of Ti-Cu sintered alloys.
abstract: The cell toxicity and the cell function of Ti-Cu sintered alloys with different Cu contents (2, 5, 10 and 25 wt.%, respectively) have been investigated in comparison with commercial pure titanium in order to assess the influence of Cu content on the cell biocompatibility of the Ti-Cu alloys. The cytotoxicity was studied by examining the MG63 cell response by CCK8 assessment. The cell morphology was evaluated by acridine orange/ethidium bromide (AO/EB) fluorescence and observed under scanning electronic microscopy (SEM). The cell function was monitored by measuring the AKP activity. It has been shown by the AO/EB morphology results that the cell death on both cp-Ti sample and Ti-Cu samples is due to apoptosis rather than necrosis. Although more apoptotic cells were found on the Ti-2Cu and Ti-5Cu samples, no evidence of Cu content dependent manner of apoptosis has been found. SEM observation indicated very good cell adhesion and spread on the cp-Ti sample and the Ti-Cu samples with different Cu contents. CCK8 results displayed that increase in the Cu content in Ti-Cu alloys does not bring about any difference in the cell viability. In addition, AKP test results indicated that no difference in the differentiation of MG63 was found between the cp-Ti and the Ti-Cu samples and among the Ti-Cu samples. All results indicated that Ti-Cu alloys exhibit very good cell biocompatibility and the Cu content up to 25 wt.% in the Ti-Cu alloys has no influence on the cell proliferation and differentiation.
authors.affiliation: Key Lab. for Anisotropy and Texture of Materials, Education Ministry of China, Northeastern University, Shenyang 110819, PR China; Jiamusi University, Jiamusi 154007, PR China. Electronic address: zhangel@atm.neu.edu.cn.
authors.forename: Erlin
authors.initials: E
authors.lastname: Zhang
authors.affiliation: Jiamusi University, Jiamusi 154007, PR China.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Key Lab. for Anisotropy and Texture of Materials, Education Ministry of China, Northeastern University, Shenyang 110819, PR China; Dept. of Prosthodontics, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Dept. of Prosthodontics, School of Stomatology, China Medical University, Liaoning Institute of Dental Research, Shenyang 110001, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.affiliation: Jiamusi University, Jiamusi 154007, PR China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D001672:Biocompatible Materials / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry / Q000633:toxicity*; D006801:Humans; D014025:Titanium / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D003300:Copper; D014025:Titanium
keywords: AKP; Biocompatibility; Cell toxicity; Implant materials; Morphology; Ti–Cu alloy