title: Advancing the detection of maternal haematopoietic microchimeric cells in fetal immune organs in mice by flow cytometry.
abstract: Maternal microchimerism, which occurs naturally during gestation in hemochorial placental mammals upon transplacental migration of maternal cells into the fetus, is suggested to significantly influence the fetal immune system. In our previous publication, we explored the sensitivity of quantitative polymerase chain reaction and flow cytometry to detect cellular microchimerism. With that purpose, we created mixed cells suspensions in vitro containing reciprocal frequencies of wild type cells and cells positive for enhanced green fluorescent protein or CD45.1(+), respectively. Here, we now introduce the H-2 complex, which defines the major histocompatibility complex in mice and is homologous to HLA in human, as an additional target to detect maternal microchimerism among fetal haploidentical cells. We envision that this advanced approach to detect maternal microchimeric cells by flow cytometry facilitates the pursuit of phenotypic, gene expression and functional analysis of microchimeric cells in future studies.
authors.affiliation: a Laboratory for Experimental Feto-maternal Medicine; Department of Obstetrics and Fetal Medicine ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.
authors.forename: Maria Emilia
authors.initials: ME
authors.lastname: Solano
authors.affiliation: a Laboratory for Experimental Feto-maternal Medicine; Department of Obstetrics and Fetal Medicine ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.
authors.forename: Kristin
authors.initials: K
authors.lastname: Thiele
authors.affiliation: a Laboratory for Experimental Feto-maternal Medicine; Department of Obstetrics and Fetal Medicine ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.
authors.forename: Ina Annelies
authors.initials: IA
authors.lastname: Stelzer
authors.affiliation: b Department of Immunology ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.
authors.forename: Hans-Willi
authors.initials: HW
authors.lastname: Mittrücker
authors.affiliation: a Laboratory for Experimental Feto-maternal Medicine; Department of Obstetrics and Fetal Medicine ; University Medical Center Hamburg-Eppendorf ; Hamburg , Germany.
authors.forename: Petra Clara
authors.initials: PC
authors.lastname: Arck
mesh_terms: D000818:Animals; D046528:Chimerism; D005260:Female; D005333:Fetus / Q000196:embryology / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007107:Immune System / Q000196:embryology* / Q000276:immunology / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000032:analysis; D008285:Major Histocompatibility Complex; D008431:Maternal-Fetal Exchange; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011247:Pregnancy
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D017493:Leukocyte Common Antigens
keywords: fetal immune development; flow cytometry; maternal microchimerism; mouse model