title: [Effect of mechanical stress on human parametrial ligament fibroblasts apoptosis].
abstract: OBJECTIVE
  To explore the possible mechanism of mechanical factors in the pathogenesis of pelvic organ prolapse (POP).
  
  
  METHODS
  The experiment material were uterosacral ligament and cardinal ligament- derived fibroblast from 10 patients who received total hysterectomy due to benign disease except POP. Fibroblast were cultured after collagenase digestion and identified by morphology and immocytochemical methods. Fibroblasts of 4-8 generations were stretched by four-point bending system for 0µ (0 mm), 1 333 µ (1 mm), 2 666 µ (2 mm), 5 333 µ (4 mm) strain, using 0 mm strain as the control group. Parameters was set to a frequency of 0.1 Hz, 4 hours. Cell apoptosis was tested by flow cytometry.
  
  
  RESULTS
  Mechanical strain increased cell apoptosis [0 µ: (7.4 ± 1.5)%, 1 333 µ: (8.7 ± 2.2)%, 2 666 µ: (19.4 ± 3.4)%, 5333 µ: (50.9 ± 6.6)%, respectively]. Cell apoptosis rate was significantly higher under strong mechanical strain (2 666 µ and 5 333 µ versus 0 µ, all P < 0.05).
  
  
  CONCLUSIONS
  Increased mechanical stress loading on human parametrial ligament fibroblasts could raise the rate of apoptosis.
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Ding
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China. Email: drhongli7777@gmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Debin
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Bingshu
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007044:Hysterectomy; D008022:Ligaments; D056887:Pelvic Organ Prolapse / Q000473:pathology*; D013314:Stress, Mechanical