title: Simultaneous detection of two breast cancer-related miRNAs in tumor tissues using p19-based disposable amperometric magnetobiosensing platforms.
abstract: A novel magnetobiosensing approach for the rapid and simultaneous detection of two breast cancer-related miRs (miR-21 and miR-205) is reported. It involves the use of antimiR-21 and antimiR-205 specific probes, chitin-modified magnetic beads (Chitin-MBs), the p19 viral protein as capture bioreceptor and amperometric detection with the H2O2/hydroquinone (HQ) system at dual screen-printed carbon electrodes (SPdCEs). The use of SPdCEs allows the simultaneous independent amperometric readout for each target miR to be measured. The magnetosensor exhibited sensitive and selective detection with dynamic ranges from 2.0 to 10.0nM and detection limits of 0.6nM (6fmol) for both target miRs without any amplification step in less than 2h. The usefulness of the approach was evaluated by detecting the endogenous levels of both target miRs in total RNA (RNAt) extracted from metastatic breast cancer cell lines and human tissues.
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: R M
authors.initials: RM
authors.lastname: Torrente-Rodríguez
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: susanacr@quim.ucm.es.
authors.forename: S
authors.initials: S
authors.lastname: Campuzano
authors.affiliation: Departamento de Medicina Celular y Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, E-28040 Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: López-Hernández
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: V Ruiz-Valdepeñas
authors.initials: VR
authors.lastname: Montiel
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Barderas
authors.affiliation: Pathology Department, University Hospital of Getafe, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Granados
authors.affiliation: Departamento de Medicina Celular y Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, E-28040 Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Sánchez-Puelles
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, E-28040 Madrid, Spain. Electronic address: pingarro@quim.ucm.es.
authors.forename: J M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008280:Magnetics / Q000295:instrumentation; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D014758:Viral Core Proteins / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D014758:Viral Core Proteins; C534425:MIR205, human; C524940:MIRN21 microRNA, human
keywords: Cancer samples; Magnetic beads; SPdCEs; Simultaneous detection; miR-205; miR-21; p19