title: Semi-automated selection of cryo-EM particles in RELION-1.3.
abstract: The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: scheres@mrc-lmb.cam.ac.uk.
authors.forename: Sjors H W
authors.initials: SH
authors.lastname: Scheres
mesh_terms: D000465:Algorithms; D020285:Cryoelectron Microscopy; D006433:Hemocyanins / Q000648:ultrastructure; D021621:Imaging, Three-Dimensional; D008958:Models, Molecular; D020836:Protein Structure, Quaternary; D012984:Software; D001616:beta-Galactosidase / Q000648:ultrastructure
chemical_list: D006433:Hemocyanins; D001616:beta-Galactosidase; C032808:keyhole-limpet hemocyanin
keywords: Automated particle picking; Electron cryo-microscopy; Single-particle analysis