title: Fabrication of nano-silver particles using Cymodocea serrulata and its cytotoxicity effect against human lung cancer A549 cells line.
abstract: The present study reports, green synthesis of bioactive silver nanoparticles (AgNPs) under different temperature (60°C, room temperature and 4° refrigerator) using the aqueous extract of sea grass Cymodocea serrulata as a potential bioreductant. Increased temperature fabricates more AgNPs compare to room temperature and refrigerator condition. At first the reduction of Ag(+) ions were confirmed through color change which produces an absorbance spectra at 420nm in UV-Visible spectrophotometer. Additionally various exclusive instrumentations such as X-ray diffraction (XRD), Dynamic light scattering (DLS), scanning electron microscope (SEM) analysis and Transmission electron microscope (TEM) were authorizes the biosynthesis and physio-chemical characterization of AgNPs. From Fourier transform infrared spectroscopy (FTIR) analysis, it was identified that the water soluble fractions of the sea grass mainly responsible for reduction of ionic silver (Ag(+)) into (Ag(0)) nano-ranged particles and also they act as stabilizing agent to sustain the durability of NPs for long period of time. Further, synthesized AgNPs shows potential cytotoxicity against human lung cancer A549 cells (LD50-100μg/ml). The overall results suggest that C. serrulata is a valuable bioresource to generate rapid and eco-friendly bioactive AgNPs towards cancer therapy.
authors.affiliation: Department of Environmental Science and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea; Department of Biochemistry, J.J. College of Arts and Science (Autonomous), Pudukkottai 622 422, Tamilnadu, India. Electronic address: biopalani82@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Palaniappan
authors.affiliation: Department of Biotechnology and Genetic Engineering, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Sathishkumar
authors.affiliation: Department of Biochemistry, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Sankar
mesh_terms: D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D020128:Inhibitory Concentration 50; D053768:Metal Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D006109:Poaceae / Q000737:chemistry*; D012834:Silver / Q000633:toxicity*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D010936:Plant Extracts; D012834:Silver
keywords: Absorbance spectra; Cymodocea serrulata; Cytotoxicity; SEM; Silver nanoparticles