title: Function of a novel C-type lectin with two CRD domains from Macrobrachium rosenbergii in innate immunity.
abstract: C-type lectins play crucial roles in innate immunity. In the present study, a novel C-type lectin gene, designated as MrCTL, was identified from Macrobrachium rosenbergii. MrCTL contains 2 carbohydrate-recognition domains (CRDs), namely MrCRD1 and MrCRD2. The MrCRD1 contains a QEP motif and MrCRD2 contains a motif of EPD. MrCTL was mainly expressed in the hepatopancreas. The expression level of MrCTL in hepatopancreas was significantly upregulated after a challenge with Vibrio parahaemolyticus or White spot syndrome virus (WSSV). The recombinant MrCTL, MrCRD1 and MrCRD2 have an ability to agglutinate both Gram-negative (V. parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) in a calcium dependent manner. The recombinant MrCTL, MrCRD1 and MrCRD2 bind directly to all tested microorganisms. All these results suggested that MrCTL may have important roles in immune defense against invading pathogens in prawns.
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Shi
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: renqian0402@126.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Ren
authors.affiliation: Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing 210046, China. Electronic address: njnuwang@263.net.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000145:classification / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001665:Binding Sites / Q000276:immunology; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions / Q000276:immunology; D007113:Immunity, Innate / Q000276:immunology*; D037181:Lectins, C-Type / Q000145:classification / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000276:immunology* / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013211:Staphylococcus aureus / Q000276:immunology; D014736:Vibrio parahaemolyticus / Q000276:immunology / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000502:physiology
chemical_list: D060829:Arthropod Proteins; D037181:Lectins, C-Type; D011994:Recombinant Proteins
keywords: C-type lectin; Innate immunity; Macrobrachium rosenbergii; Pattern recognition receptor