title: Psychrobacter ciconiae sp. nov., isolated from white storks (Ciconia ciconia).
abstract: Five beige bacterial strains (176/10(T), 178/10, 182/10, 185/7 and 193/8) were isolated from white storks in Poland and found to share identical 16S rRNA gene sequences; they were also investigated in a polyphasic taxonomic study. The cells of all isolates were rod-shaped and Gram-stain-negative. A comparison of the 16S rRNA gene sequences of these organisms with the sequences of the type strains of the most closely related species of the genus Psychrobacter showed highest sequence similarities to the type strains of Psychrobacter pulmonis and Psychrobacter faecalis (both 97.1 %). The 16S rRNA gene sequence similarities to all other species of the genus Psychrobacter were below 96.3 %. All five isolates showed an identical profile of physiological reactions and almost identical fatty acid profiles consisting of mainly C18 : 1ω9c, but also C12 : 0 3-OH as a characteristic hydroxylated fatty acid. A quinone system with mainly ubiquinone Q-8 was detected and the polar lipid profile of the type strain, 176/10(T), was mainly composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine and diphosphatidylglycerol, plus some hitherto uncharacterized phospholipids and one aminolipid. The major polyamines were spermidine and putrescine. DNA-DNA hybridizations between 176/10(T) and the type strains of P. pulmonis and P. faecialis resulted in relatedness values below 70 %. These results indicate that the strains represent a novel species, for which the name Psychrobacter ciconiae sp. nov. (type strain 176/10(T) = CIP 110777(T) = LMG 28175(T) = CCM 8519(T)) is proposed.
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kämpfer
authors.affiliation: Wydział Nauk Biologicznych, Uniwersytet Zielonogórski, Zielona Góra, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Jerzak
authors.affiliation: Robert Koch-Institut, Bereich Wernigerode, Wernigerode, Germany.
authors.forename: Gottfried
authors.initials: G
authors.lastname: Wilharm
authors.affiliation: Robert Koch-Institut, Bereich Wernigerode, Wernigerode, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Golke
authors.affiliation: Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Wien, Austria.
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.affiliation: Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany.
authors.forename: Stefanie P
authors.initials: SP
authors.lastname: Glaeser
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001717:Birds / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D011044:Poland; D044096:Psychrobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D011700:Putrescine / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013095:Spermidine / Q000737:chemistry; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8; D013095:Spermidine; D011700:Putrescine