title: Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources.
abstract: This study characterized carriage and clinical pneumococcal isolates for serotypes, penicillin susceptibility, virulence genes and restriction fragment length polymorphism (RFLP) pattern of penicillin binding protein (PBP) genes. DNA fingerprint of isolates was generated by BOX-PCR. Majority of serotypes were 23F followed by 19F, 19A and 6A. Twenty-four percent of isolates were penicillin non-susceptible (PNSP). All of the targeted virulence genes were detected in all isolates with the exception of pili; 20.6% (n=22) for PI-1 and 14.0% (n=15) for PI-2. Of the 13 isolates which carried both PI-1 and PI-2, 10 were of clinical origin. Digested pbp-DNA produced three PBP-RFLP profiles for pbp1a (A1 to A3), six profiles for pbp2b (B1 to B6) and seven for pbp2x (X1 to X7) mostly in PNSPs. Based on BOX-PCR analysis, the majority of isolates were genetically diverse with a small number of potentially related isolates carrying pili genes. No obvious genotypic association was observed pertaining to carriage and clinical origin of isolates.
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; Department of Pathology, Selayang Hospital, Selayang, Selangor, Malaysia.
authors.forename: Nik Noorul Shakira Mohamed
authors.initials: NN
authors.lastname: Shakrin
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Siti Norbaya
authors.initials: SN
authors.lastname: Masri
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Niazlin Mohd
authors.initials: NM
authors.lastname: Taib
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Syafinaz Amin
authors.initials: SA
authors.lastname: Nordin
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Farida
authors.initials: F
authors.lastname: Jamal
authors.affiliation: Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia; Halal Products Research Institute, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. Electronic address: mnasir@upm.edu.my.
authors.forename: Mohd Nasir Mohd
authors.initials: MN
authors.lastname: Desa
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002353:Carrier State; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics*; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010403:Penicillin Resistance / Q000235:genetics*; D046915:Penicillin-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology; D010802:Phylogeny; D011018:Pneumonia, Pneumococcal / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D010406:Penicillins
keywords: BOX-PCR; PBP-RFLP; Pili; Serotype; Streptococcus pneumoniae; Vaccine; Virulence