title: Crystallization and preliminary X-ray diffraction analysis of the Sel1-like repeats of SEL1L.
abstract: Terminally misfolded or unassembled proteins are selectively recognized and cleared by the ER-associated degradation (ERAD) pathway. Suppressor/enhancer of lin-12-like (SEL1L), a component of the dislocation machinery containing the E3 ubiquitin ligase Hrd1, plays an important role in selecting and transporting ERAD substrates for degradation in the endoplasmic reticulum. In this study, the purification, crystallization and preliminary X-ray diffraction analysis of recombinant mouse SEL1L (residues 348-533) are reported. The crystals were obtained by the hanging-drop vapour-diffusion method at pH 8.5 and 277 K using 30% 2-propanol as a precipitant. Optimized crystals diffracted to 3.3 Å resolution at a synchrotron-radiation source. Preliminary X-ray diffraction analysis revealed that the crystals belonged to space group P21 and contained four molecules per asymmetric unit, with a solvent content of 44%.
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.
authors.forename: Hanbin
authors.initials: H
authors.lastname: Jeong
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.
authors.forename: Hakbong
authors.initials: H
authors.lastname: Lee
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 689-798, Republic of Korea.
authors.forename: Changwook
authors.initials: C
authors.lastname: Lee
mesh_terms: D000818:Animals; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry*
chemical_list: D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; C492169:Sel1h protein, mouse
keywords: SEL1L; Sel1-like repeats