title: Comparison of multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC) in single-cell sequencing.
abstract: Single-cell sequencing promotes our understanding of the heterogeneity of cellular populations, including the haplotypes and genomic variability among different generation of cells. Whole-genome amplification is crucial to generate sufficient DNA fragments for single-cell sequencing projects. Using sequencing data from single sperms, we quantitatively compare two prevailing amplification methods that extensively applied in single-cell sequencing, multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC). Our results show that MALBAC, as a combination of modified MDA and tweaked PCR, has a higher level of uniformity, specificity and reproducibility.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China; BGI-Shenzhen, Shenzhen, 518083, China.
authors.forename: Minfeng
authors.initials: M
authors.lastname: Chen
authors.affiliation: The fourth people's hospital of Shenzhen (Futian hospital), Shenzhen, 518033, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Song
authors.affiliation: School of Computer, National University of Defense Technology, Changsha, 410073, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zou
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Hu
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China; Department of Molecular Medicine, Aarhus University Hospital, Aarhus, Denmark.
authors.forename: Shancen
authors.initials: S
authors.lastname: Zhao
authors.affiliation: BGI-Shenzhen, Shenzhen, 518083, China; State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
authors.forename: Shengjie
authors.initials: S
authors.lastname: Gao
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ling
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D015894:Genome, Human; D023281:Genomics / Q000379:methods; D006801:Humans; D008297:Male; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015415:Biomarkers