title: Differential expression of mucosal trefoil factors and mucins in pediatric inflammatory bowel diseases.
abstract: In the intestinal mucosa trefoil factors (TFF) and mucins (Muc) - primarily produced by goblet cells - are thought to play a major role in providing barrier function during infection and inflammation. To investigate their role in pediatric Crohn's disease (CD) and ulcerative colitis (UC) we obtained mucosal biopsies of children with CD, UC and healthy controls and analyzed genetic expression. Levels of TFF2 mRNA were lower in inflamed mucosal samples (terminal ileum (TI) and duodenum) of children with CD, but higher in non-inflamed mucosal samples when compared to healthy controls (p < 0.05). Similarly, TFF2 levels in the TI were significantly lower in inflamed UC tissue. Adjustment for goblet cell density revealed slightly less marked, yet significantly different gene expression in IBD and controls. Furthermore, TI expression of TFF2 and Muc2 was inversely correlated with interleukin-8 expression in CD (p = 0.027). In Summary, our data demonstrate significant changes in Muc and TFF mRNA expression in pediatric patients with IBD suggesting a role in mucosal healing. Further studies are needed to elucidate a potential use as biomarkers for disease progression.
authors.affiliation: Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Kai O
authors.initials: KO
authors.lastname: Hensel
authors.affiliation: Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Boland
authors.affiliation: Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Postberg
authors.affiliation: 1] Department of Pediatric Gastroenterology, Hepatology and Nutrition, Addenbrooke's Hospital, Cambridge, UK [2] University Department of Pediatrics, University of Cambridge, Cambridge, UK.
authors.forename: Matthias
authors.initials: M
authors.lastname: Zilbauer
authors.affiliation: Department of Pediatric Gastroenterology, Hepatology and Nutrition, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Heuschkel
authors.affiliation: Department of Pathology, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Silvia
authors.initials: S
authors.lastname: Vogel
authors.affiliation: Department of Pathology, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gödde
authors.affiliation: Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wirth
authors.affiliation: Department of Pediatrics, HELIOS Medical Center Wuppertal, Faculty of Health, Witten/Herdecke University, Germany.
authors.forename: Andreas C
authors.initials: AC
authors.lastname: Jenke
mesh_terms: D000293:Adolescent; D015415:Biomarkers / Q000378:metabolism; D002648:Child; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D009077:Mucins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution; D000071164:Trefoil Factor-2
chemical_list: D015415:Biomarkers; D009077:Mucins; D010455:Peptides; C000604007:TFF2 protein, human; D000071164:Trefoil Factor-2