title: Effect of long-term different fertilization on bacterial community structures and diversity in citrus orchard soil of volcanic ash.
abstract: This study was conducted to assess bacterial species richness, diversity and community distribution according to different fertilization regimes for 16 years in citrus orchard soil of volcanic ash. Soil samples were collected and analyzed from Compost (cattle manure, 2,000 kg/10a), 1/2 NPK+compost (14-20-14+2,000 kg/10a), NPK+compost (28-40-28+2,000 kg/10a), NPK (28-40-28 kg/10a), 3 NPK (84-120-84 kg/10a), and Control (no fertilization) plot which have been managed in the same manners with compost and different amount of chemical fertilization. The range of pyrosequencing reads and OTUs were 4,687-7,330 and 1,790-3,695, respectively. Species richness estimates such as Ace, Chao1, and Shannon index were higher in 1/2 NPK+compost than other treatments, which were 15,202, 9,112, 7.7, respectively. Dominant bacterial groups at level of phylum were Proteobacteria, Acidobacteria, and Actinobacteria. Those were occupied at 70.9% in 1/2 NPK+compost. Dominant bacterial groups at level of genus were Pseudolabrys, Bradyrhizobium, and Acidobacteria. Those were distributed at 14.4% of a total of bacteria in Compost. Soil pH displayed significantly closely related to bacterial species richness estimates such as Ace, Chao1 (p<0.05) and Shannon index (p<0.01). However, it showed the negative correlation with exchangeable aluminum contents (p<0.05). In conclusion, diversity of bacterial community in citrus orchard soil was affected by fertilization management, soil pH changes and characteristics of volcanic ash.
authors.affiliation: National Institute of Horticultural & Herbal Science, RDA, Jeju, 690-150, Republic of Korea, choa0313@korea.kr.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Joa
authors.forename: Hang Yeon
authors.initials: HY
authors.lastname: Weon
authors.forename: Hae Nam
authors.initials: HN
authors.lastname: Hyun
authors.forename: Young Chull
authors.initials: YC
authors.lastname: Jeun
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Koh
mesh_terms: D061271:Acidobacteria / Q000145:classification / Q000302:isolation & purification; D039903:Actinobacteria / Q000145:classification / Q000302:isolation & purification; D000383:Agriculture; D020561:Alphaproteobacteria / Q000145:classification / Q000302:isolation & purification; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D002417:Cattle; D002957:Citrus; D005308:Fertilizers / Q000009:adverse effects*; D008372:Manure; D020560:Proteobacteria / Q000145:classification / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D018707:Volcanic Eruptions
chemical_list: D005308:Fertilizers; D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil