title: Stiffness-dependent in vitro uptake and lysosomal acidification of colloidal particles.
abstract: The physico-chemical properties of colloidal particles determine their uptake into cells. For a series of microparticles only one parameter, the mechanical stiffness, was varied, whereas other parameters such as size, shape, and charge were kept constant. The uptake was monitored in situ by analyzing individual particle trajectories including the progress of endocytosis, derived from local pH measurements around each particle. Evidence is presented that soft particles with low stiffness are transported faster to lysosomes than stiffer ones.
authors.affiliation: Fachbereich Physik, Philipps Universität Marburg, 35037 Marburg (Germany).
authors.forename: Raimo
authors.initials: R
authors.lastname: Hartmann
authors.forename: Marcel
authors.initials: M
authors.lastname: Weidenbach
authors.forename: Martin
authors.initials: M
authors.lastname: Neubauer
authors.forename: Andreas
authors.initials: A
authors.lastname: Fery
authors.forename: Wolfgang J
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D002214:Capsules / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D003102:Colloids / Q000737:chemistry / Q000378:metabolism*; D055119:Elastic Modulus; D004573:Electrolytes / Q000737:chemistry; D004705:Endocytosis; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism*; D061986:MCF-7 Cells; D018625:Microscopy, Atomic Force; D053758:Nanoparticles / Q000737:chemistry
chemical_list: D002214:Capsules; D003102:Colloids; D004573:Electrolytes
keywords: biosensors; mechanical properties; nanoparticles; polymeric capsules