title: Tapping a bacterial enzymatic pathway for the preparation and manipulation of synthetic nanomaterials.
abstract: We present a spherical micelle generated in a three-step sequence in which a farnesyl-pantetheine conjugate is phosphorylated, adenylated, and phosphorylated once more to generate a farnesyl-CoA amphiphile that self-assembles into spherical micelles. A sphere-to-fibril morphological switch is achieved by enzymatically transferring the farnesyl group of the farnesyl-CoA micelle onto a peptide via phosphopantetheinyl transferase to generate a peptide amphiphile. Each step in the sequence is followed with characterization by HPLC, MS, TEM, and DLS. This system offers an entry into cofactor-mediated peptide decoration by extending the principles of bioresponsive polymeric materials to sequential enzyme cascades.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego , 9500 Gilman Drive, La Jolla, California 92093, United States.
authors.forename: Ti-Hsuan
authors.initials: TH
authors.lastname: Ku
authors.forename: Swagat
authors.initials: S
authors.lastname: Sahu
authors.forename: Nicolas M
authors.initials: NM
authors.lastname: Kosa
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Pham
authors.forename: Michael D
authors.initials: MD
authors.lastname: Burkart
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Gianneschi
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D005204:Farnesol / Q000737:chemistry; D008823:Micelles; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010204:Pantetheine / Q000737:chemistry
chemical_list: D008823:Micelles; D005204:Farnesol; D010204:Pantetheine