title: Cleaved DNAzyme substrate induced enzymatic cascade for the exponential amplified analysis of L-histidine.
abstract: A novel strategy of cleaved DNAzyme substrate induced enzymatic cascade has been devised for the exponential amplified detection of L-histidine. The enzyme strand carries out hydrolytic cleavage of the substrate strand in the presence of L-histidine. The cleaved DNAzyme substrates introduce the polymerase/endonuclease reaction cycles as primers. The L-histidine acts as the activator for enzymatic cascade amplification generating a distinguishable fluorescence enhancement. A good nonlinear correlation (R=0.9994) between fluorescence intensity and the logarithm of the L-histidine concentration is obtained over the range from 50 nM to 1.0 mM. The detection limit was estimated as 30 nM. This efficient amplification of the fluorescence signal is attributed to the L-histidine induced cooperation of Klenow Fragment polymerase (exo(-)) and Nb.BbvCI endonuclease reaction. The activation of such enzymatic cascades through analyte-DNAzyme interactions has a substantial impact on the development of exponential amplified DNAzyme sensors.
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China. Electronic address: jinglin_he@hotmail.com.
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: He
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China.
authors.forename: Shuang-Li
authors.initials: SL
authors.lastname: Zhu
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Li
authors.affiliation: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Xiang
authors.affiliation: Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, PR China. Electronic address: zhongcao2004@163.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Cao
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D004256:DNA Polymerase I / Q000737:chemistry*; D021881:DNA, Catalytic / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000737:chemistry*; D005453:Fluorescence; D006639:Histidine / Q000032:analysis*; D006868:Hydrolysis; D057230:Limit of Detection; D009841:Oligonucleotides / Q000737:chemistry*; D012996:Solutions
chemical_list: D021881:DNA, Catalytic; D009841:Oligonucleotides; D012996:Solutions; D006639:Histidine; D004256:DNA Polymerase I; D004720:Endonucleases
keywords: Enzymatic cascade; Exponential amplified analysis; Self-cleaved DNAzyme; l-Histidine