title: Intermittent access to liquid sucrose differentially modulates energy intake and related central pathways in control or high-fat fed mice.
abstract: Intake of sodas has been shown to increase energy intake and to contribute to obesity in humans and in animal models, although the magnitude and importance of these effects are still debated. Moreover, intake of sugar sweetened beverages is often associated with high-fat food consumption in humans. We studied two different accesses to a sucrose-sweetened water (SSW, 12.3%, a concentration similar to that usually found in sugar sweetened beverages) in C57BL/6 mice fed a normal-fat (NF) or a high-fat (HF) diet in a scheduled access (7.5h). NF-fed and HF-fed mice received during 5weeks access to water, to SSW continuously for 7.5h (SSW), or to water plus SSW for 2h (randomly-chosen time slot for only 5 random days/week) (SSW-2h). Mouse preference for SSW was greater in HF-fed mice than NF-fed mice. Continuous SSW access induced weight gain whatever the diet and led to greater caloric intake than mice drinking water in NF-fed mice and in the first three weeks in HF-fed mice. In HF-fed mice, 2h-intermittent access to SSW induced a greater body weight gain than mice drinking water, and led to hyperphagia on the HF diet when SSW was accessible compared to days without SSW 2h-access (leading to greater overall caloric intake), possibly through inactivation of the anorexigenic neuropeptide POMC in the hypothalamus. This was not observed in NF-fed mice, but 2h-intermittent access to SSW stimulated the expression of dopamine, opioid and endocannabinoid receptors in the nucleus accumbens compared to water-access. In conclusion, in mice, a sucrose solution provided 2h-intermittently and a high-fat diet have combined effects on peripheral and central homeostatic systems involved in food intake regulation, a finding which has significant implications for human obesity.
authors.affiliation: AgroParisTech, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France. Electronic address: marionjournel@gmail.com.
authors.forename: Marion
authors.initials: M
authors.lastname: Soto
authors.affiliation: INRA, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Chaumontet
authors.affiliation: INRA, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Even
authors.affiliation: AgroParisTech, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France; INRA, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Nachiket
authors.initials: N
authors.lastname: Nadkarni
authors.affiliation: AgroParisTech, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Piedcoq
authors.affiliation: AgroParisTech, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Darcel
authors.affiliation: AgroParisTech, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Tomé
authors.affiliation: INRA, CRNH-IdF, UMR0914 Nutrition Physiology and Ingestive Behavior, F-75005 Paris, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Fromentin
mesh_terms: D000818:Animals; D001823:Body Composition / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism; D002766:Cholecystokinin / Q000097:blood; D059305:Diet, High-Fat; D004327:Drinking Behavior / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects*; D005247:Feeding Behavior / Q000187:drug effects*; D005508:Food Deprivation; D005786:Gene Expression Regulation / Q000187:drug effects*; D054439:Ghrelin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019894:Peptide YY / Q000097:blood; D013395:Sucrose / Q000008:administration & dosage*; D013549:Sweetening Agents / Q000008:administration & dosage*; D013997:Time Factors
chemical_list: D054439:Ghrelin; D020738:Leptin; D013549:Sweetening Agents; D019894:Peptide YY; D013395:Sucrose; D002766:Cholecystokinin
keywords: Binge-like drinking; Energy balance; Intermittent access; Mice; Reward gene expression; Scheduled feeding; Sucrose solution