title: Occurrence of ochratoxin A in poultry feeds and feed ingredients from Pakistan.
abstract: A study was conducted to evaluate the occurrence of ochratoxin A (OTA) in complete poultry feeds (n=80) and poultry feed ingredients (n=286) from Pakistan. All samples were first analyzed by indirect enzyme linked immunosorbent assay (ELISA), samples with OTA concentrations above the European Union maximum regulatory limit (MRL, 100 μg/kg) were further confirmed by HPLC-FLD. Contamination frequency and mean OTA levels were 31% and 51 μg/kg in feed ingredients, and the corresponding values for complete feeds were 38% and 75 μg/kg. Ten samples of complete poultry feed and 19 samples of feed ingredients contained OTA at levels higher than the MRL. The results of the present study indicate that there is a strong need for a more intense monitoring programs for OTA in poultry feed.
authors.affiliation: National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan, tufail.sherazi@yahoo.com.
authors.forename: S T H
authors.initials: ST
authors.lastname: Sherazi
authors.forename: Z H
authors.initials: ZH
authors.lastname: Shar
authors.forename: G A
authors.initials: GA
authors.lastname: Sumbal
authors.forename: Eddie T
authors.initials: ET
authors.lastname: Tan
authors.forename: M I
authors.initials: MI
authors.lastname: Bhanger
authors.forename: Huseyin
authors.initials: H
authors.lastname: Kara
authors.forename: S M
authors.initials: SM
authors.lastname: Nizamani
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D059022:Food Safety / Q000379:methods; D009793:Ochratoxins / Q000032:analysis*; D010154:Pakistan; D011200:Poultry
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A