title: Histological and lectin histochemical studies on the olfactory mucosae of the Korean roe deer, Capreolus pygargus.
abstract: The morphological features of the olfactory mucosae of Korean roe deer, Capreolus pygargus, were histologically studied using the ethmoid turbinates containing the olfactory mucosae from six roe deer (male, 2-3 years old). The ethmoid turbinates were embedded in paraffin, and histochemically evaluated in terms of the mucosal characteristics. Lectin histochemistry was performed to investigate the carbohydrate-binding specificity on the olfactory mucosa. Lectins, including Triticum vulgaris wheat germ agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), and soybean agglutinin (SBA) were used for the N-acetylglucosamine, fucose and N-acetylgalactosamine carbohydrate groups, respectively. Histologically, the olfactory mucosa, positioned mainly in the caudal roof of the nasal cavity, consisted of the olfactory epithelium and the lamina propria. The olfactory epithelium consisted of protein gene product (PGP) 9.5-positive olfactory receptor cells, galectin-3-positive supporting cells and basal cells. Bowman's glands in the lamina propria were stained by both the periodic acid Schiff reagent and alcian blue (pH 2.5). Two types of lectin, WGA and SBA, were labeled in free border, receptor cells, supporting cells and Bowman's glands, with the exception of basal cells, while UEA-I was labeled in free border, supporting cells and Bowman's glands, but not in receptor cells and basal cells, suggesting that carbohydrate terminals on the olfactory mucosae of roe deer vary depending on cell type. This is the first morphological study of the olfactory mucosa of the Korean roe deer to evaluate carbohydrate terminals in the olfactory mucosae.
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Changnam
authors.initials: C
authors.lastname: Park
authors.affiliation: School of Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Meejung
authors.initials: M
authors.lastname: Ahn
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Jeongtae
authors.initials: J
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Seungjoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine and Animal Medical Institute, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: moonc@chonnam.ac.kr.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.affiliation: Laboratory of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: shint@jejunu.ac.kr.
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000117:Acetylglucosamine / Q000378:metabolism; D000818:Animals; D003670:Deer / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D037102:Lectins / Q000378:metabolism*; D008297:Male; D009831:Olfactory Mucosa / Q000378:metabolism*
chemical_list: D037102:Lectins; D000116:Acetylgalactosamine; D000117:Acetylglucosamine
keywords: Lectin; Olfactory epithelium; Olfactory mucosa; Roe deer