title: Application of laser microdissection ICP-MS for high resolution elemental mapping in mouse brain tissue: a comparative study with laser ablation ICP-MS.
abstract: Mapping of elements in biological tissue by laser induced mass spectrometry is a fast growing analytical methodology in life sciences. This method provides a multitude of useful information of metal, nonmetal, metalloid and isotopic distribution at major, minor and trace concentration ranges, usually with a lateral resolution of 12-160 µm. Selected applications in medical research require an improved lateral resolution of laser induced mass spectrometric technique at the low micrometre scale and below. The present work demonstrates the applicability of a recently developed analytical methodology - laser microdissection associated to inductively coupled plasma mass spectrometry (LMD ICP-MS) - to obtain elemental images of different solid biological samples at high lateral resolution. LMD ICP-MS images of mouse brain tissue samples stained with uranium and native are shown, and a direct comparison of LMD and laser ablation (LA) ICP-MS imaging methodologies, in terms of elemental quantification, is performed.
authors.affiliation: Zentralinstitut für Engineering, Elektronik und Analytik, Analytik (ZEA-3), Forschungszentrum Jülich, D-52425 Jülich, Germany. Electronic address: sussulini@iqm.unicamp.br.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Sussulini
authors.affiliation: Zentralinstitut für Engineering, Elektronik und Analytik, Analytik (ZEA-3), Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors.forename: J Sabine
authors.initials: JS
authors.lastname: Becker
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D001931:Brain Mapping / Q000295:instrumentation / Q000379:methods*; D006624:Hippocampus / Q000737:chemistry* / Q000648:ultrastructure; D007501:Iron / Q000032:analysis; D061346:Laser Capture Microdissection / Q000295:instrumentation / Q000379:methods*; D053685:Laser Therapy / Q000295:instrumentation / Q000379:methods*; D008274:Magnesium / Q000032:analysis; D051379:Mice; D010758:Phosphorus / Q000032:analysis; D011188:Potassium / Q000032:analysis; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*; D013378:Substantia Nigra / Q000737:chemistry* / Q000648:ultrastructure; D014501:Uranium / Q000032:analysis; D015032:Zinc / Q000032:analysis
chemical_list: D010758:Phosphorus; D014501:Uranium; D007501:Iron; D008274:Magnesium; D015032:Zinc; D011188:Potassium
keywords: Inductively coupled plasma mass spectrometry; Laser ablation; Laser microdissection; Mass spectrometry imaging; Mouse brain; Quantification