title: SILAC-based quantitative proteomic analysis of human lung cell response to copper oxide nanoparticles.
abstract: Copper (II) oxide (CuO) nanoparticles (NP) are widely used in industry and medicine. In our study we evaluated the response of BEAS-2B human lung cells to CuO NP, using Stable isotope labeling by amino acids in cell culture (SILAC)-based proteomics and phosphoproteomics. Pathway modeling of the protein differential expression showed that CuO NP affect proteins relevant in cellular function and maintenance, protein synthesis, cell death and survival, cell cycle and cell morphology. Some of the signaling pathways represented by BEAS-2B proteins responsive to the NP included mTOR signaling, protein ubiquitination pathway, actin cytoskeleton signaling and epithelial adherens junction signaling. Follow-up experiments showed that CuO NP altered actin cytoskeleton, protein phosphorylation and protein ubiquitination level.
authors.affiliation: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi, United States of America; Department of Basic Sciences, College of Veterinary Medicine, 240 Wise Center Drive, Mississippi State University, Mississippi, United States of America.
authors.forename: Mariola J
authors.initials: MJ
authors.lastname: Edelmann
authors.affiliation: Department of Basic Sciences, College of Veterinary Medicine, 240 Wise Center Drive, Mississippi State University, Mississippi, United States of America.
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Shack
authors.affiliation: Department of Chemical Engineering, Mississippi State University, Mississippi, United States of America.
authors.forename: Caitlin D
authors.initials: CD
authors.lastname: Naske
authors.affiliation: Department of Chemical Engineering, Mississippi State University, Mississippi, United States of America.
authors.forename: Keisha B
authors.initials: KB
authors.lastname: Walters
authors.affiliation: Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi, United States of America; Department of Basic Sciences, College of Veterinary Medicine, 240 Wise Center Drive, Mississippi State University, Mississippi, United States of America.
authors.forename: Bindu
authors.initials: B
authors.lastname: Nanduri
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D056809:Alveolar Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D054875:Ubiquitination
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; D010750:Phosphoproteins; D020543:Proteome; D003300:Copper; C000520:cuprous oxide