title: In vivo analysis of a fluorescent SUMO fusion in transgenic Drosophila.
abstract: Sumoylation, the covalent attachment of SUMO, a 90 amino acid peptide related to ubiquitin, is a major modulator of protein functions. Fluorescent SUMO protein fusions have been used in cell cultures to visualize SUMO in vivo but not in multicellular organisms. We generated a transgenic line of Drosophila expressing an mCherry-SUMO fusion. We analyzed its pattern in vivo in salivary gland nuclei expressing Venus-HP1 to recognize the different chromatin components (Chromocenter, chromosome IV). We compared it to SUMO immunostaining on squashed polytene chromosomes and observed similar patterns. In addition to the previously reported SUMO localizations (chromosome arms and chromocenter), we identify 2 intense binding sites: the fourth chromosome telomere and the DAPI-bright band in the region 81F.
authors.affiliation: a Department of Genetics and Evolution; University of Geneva; Geneva, Switzerland.
authors.forename: Marion
authors.initials: M
authors.lastname: Bocksberger
authors.forename: François
authors.initials: F
authors.lastname: Karch
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Gibert
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004330:Drosophila; D008164:Luminescent Proteins; D059007:Polytene Chromosomes / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000032:analysis; D025842:SUMO-1 Protein / Q000032:analysis*; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D025842:SUMO-1 Protein; D000097573:Red Fluorescent Protein
keywords: Drosophila; SUMO; chromatin; chromosome; fluorescent fusion