title: Proteomic analysis of human placental syncytiotrophoblast microvesicles in preeclampsia.
abstract: BACKGROUND
  Placental syncytiotrophoblast microvesicles (STBM) are shed into the maternal circulation during normal pregnancy. STBM circulate in significantly increased amounts in preeclampsia (PE) and are considered to be among contributors to the exaggerated proinflammatory, procoagulant state of PE. However, protein composition of STBM in normal pregnancy and PE remains unknown. We therefore sought to determine the protein components of STBM and whether STBM protein expressions differ in preeclamptic and normal pregnancies. Patients with PE (n = 3) and normal pregnant controls (n = 6) were recruited. STBM were prepared from placental explant culture supernatant. STBM proteins were analyzed by a combination of 1D Gel-LC-MS/MS. Protein expressions levels were quantified using spectral counts and validated by immunohistochemistry.
  
  
  RESULTS
  Over 400 proteins were identified in the STBM samples. Among these, 25 proteins were found to be differentially expressed in preeclampsia compared to healthy pregnant controls, including integrins, annexins and histones.
  
  
  CONCLUSIONS
  STBM proteins include those that are implicated in immune response, coagulation, oxidative stress, apoptosis as well as lipid metabolism pathways. Differential protein expressions of STBM suggest their pathophysiological relevance in PE.
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Sonia
authors.initials: S
authors.lastname: Baig
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Narasimhan
authors.initials: N
authors.lastname: Kothandaraman
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Jayapal
authors.initials: J
authors.lastname: Manikandan
authors.affiliation: Experimental Therapeutic Center, Agency for Science, Technology and Research, 31 Biopolis Way, Singapore, 138669 Singapore.
authors.forename: Li
authors.initials: L
authors.lastname: Rong
authors.affiliation: Experimental Therapeutic Center, Agency for Science, Technology and Research, 31 Biopolis Way, Singapore, 138669 Singapore.
authors.forename: Kim Huey
authors.initials: KH
authors.lastname: Ee
authors.affiliation: Experimental Therapeutic Center, Agency for Science, Technology and Research, 31 Biopolis Way, Singapore, 138669 Singapore.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Hill
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Chin Wee
authors.initials: CW
authors.lastname: Lai
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Wan Yu
authors.initials: WY
authors.lastname: Tan
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Felicia
authors.initials: F
authors.lastname: Yeoh
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Anita
authors.initials: A
authors.lastname: Kale
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Lin Lin
authors.initials: LL
authors.lastname: Su
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Arijit
authors.initials: A
authors.lastname: Biswas
authors.affiliation: Division of Rheumatology, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Sheila
authors.initials: S
authors.lastname: Vasoo
authors.affiliation: Division of Maternal-Fetal Medicine, Department of Obstetrics & Gynaecology, Yong Loo Lin School of Medicine, National University of Singapore & National University Health System, 10 Medical Drive, Singapore, 119260 Singapore.
authors.forename: Mahesh
authors.initials: M
authors.lastname: Choolani
keywords: Placental microvesicles; Preeclampsia; Pregnancy; Proteome