title: Titanium coated with functionalized carbon nanotubes--a promising novel material for biomedical application as an implantable orthopaedic electronic device.
abstract: The aim of the study was to fabricate titanium (Ti) material coated with functionalized carbon nanotubes (f-CNTs) that would have potential medical application in orthopaedics as an implantable electronic device. The novel biomedical material (Ti-CNTs-H2O) would possess specific set of properties, such as: electrical conductivity, non-toxicity, and ability to inhibit connective tissue cell growth and proliferation protecting the Ti-CNTs-H2O surface against covering by cells. The novel material was obtained via an electrophoretic deposition of CNTs-H2O on the Ti surface. Then, physicochemical, electrical, and biological properties were evaluated. Electrical property evaluation revealed that a Ti-CNTs-H2O material is highly conductive and X-ray photoelectron spectroscopy analysis demonstrated that there are mainly COOH groups on the Ti-CNTs-H2O surface that are found to inhibit cell growth. Biological properties were assessed using normal human foetal osteoblast cell line (hFOB 1.19). Conducted cytotoxicity tests and live/dead fluorescent staining demonstrated that Ti-CNTs-H2O does not exert toxic effect on hFOB cells. Moreover, fluorescence laser scanning microscope observation demonstrated that Ti-CNTs-H2O surface retards to a great extent cell proliferation. The study resulted in successful fabrication of highly conductive, non-toxic Ti-CNTs-H2O material that possesses ability to inhibit osteoblast proliferation and thus has a great potential as an orthopaedic implantable electronic device.
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University of Lublin, Faculty of Pharmacy with Medical Analytics Division, Chodzki 1, 20-093 Lublin, Poland. Electronic address: agata.przekora@umlub.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Przekora
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-Univ. of Science and Technology, A. Mickiewicz 30 Ave., 30-059 Cracow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Benko
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-Univ. of Science and Technology, A. Mickiewicz 30 Ave., 30-059 Cracow, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Nocun
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-Univ. of Science and Technology, A. Mickiewicz 30 Ave., 30-059 Cracow, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Wyrwa
authors.affiliation: Faculty of Materials Science and Ceramics, AGH-Univ. of Science and Technology, A. Mickiewicz 30 Ave., 30-059 Cracow, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Blazewicz
authors.affiliation: Department of Biochemistry and Biotechnology, Medical University of Lublin, Faculty of Pharmacy with Medical Analytics Division, Chodzki 1, 20-093 Lublin, Poland.
authors.forename: Grazyna
authors.initials: G
authors.lastname: Ginalska
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D058266:Dielectric Spectroscopy; D004581:Electronics; D006801:Humans; D008856:Microscopy, Fluorescence; D037742:Nanotubes, Carbon / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D037742:Nanotubes, Carbon; D014025:Titanium
keywords: Bone growth sensor; CNTs; Cell culture; Electrical impedance spectroscopy; Electrophoretic deposition; X-ray photoelectron spectroscopy