title: In vivo effects of adipose-derived stem cells in inducing neuronal regeneration in Sprague-Dawley rats undergoing nerve defect bridged with polycaprolactone nanotubes.
abstract: There have been many attempts for regeneration of peripheral nerve injury. In this study, we examined the in vivo effects of non-differentiated and neuronal differentiated adipose-derived stem cells (ADSCs) in inducing the neuronal regeneration in the Sprague-Dawley (SD) rats undergoing nerve defect bridged with the PCL nanotubes. Then, we performed immunohistochemical and histopathologic examinations, as well as the electromyography, in three groups: the control group (14 sciatic nerves transplanted with the PCL nanotube scaffold), the experimental group I (14 sciatic nerves with the non-differentiated ADSCs at a density of 7×10(5) cells/0.1 mL) and the experimental group II (14 sciatic nerves with the neuronal differentiated ADSCs at 7×10(5) cells/0.1 mL). Six weeks postoperatively, the degree of the neuronal induction and that of immunoreactivity to nestin, MAP-2 and GFAP was significantly higher in the experimental group I and II as compared with the control group. In addition, the nerve conduction velocity (NCV) was significantly higher in the experimental group I and II as compared with the control group (P=0.021 and P=0.020, respectively). On the other hand, there was no significant difference in the NCV between the two experimental groups (P>0.05). Thus, our results will contribute to treating patients with peripheral nerve defects using PCL nanotubes with ADSCs.
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Dong-Yeon
authors.identifier: 0000-0002-9203-1925
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Yong-Seong
authors.identifier: 0000-0002-6108-758X
authors.initials: YS
authors.lastname: Choi
authors.affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University, Seoul, Korea.
authors.forename: Sung-Eun
authors.identifier: 0000-0001-9445-2451
authors.initials: SE
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jung-Ho
authors.identifier: 0000-0002-3800-5494
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Sue-Min
authors.identifier: 0000-0002-0894-5207
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Young-Jin
authors.identifier: 0000-0002-9046-9907
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Jong-Won
authors.identifier: 0000-0002-9398-8059
authors.initials: JW
authors.lastname: Rhie
authors.affiliation: Department of Plastic and Reconstructive Surgery, The Catholic University of Korea, Seoul, Korea.
authors.forename: Young-Joon
authors.identifier: 0000-0002-2678-9092
authors.initials: YJ
authors.lastname: Jun
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D004576:Electromyography; D005904:Glial Fibrillary Acidic Protein; D008297:Male; D043942:Nanotubes; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000276:immunology; D064231:Nestin / Q000276:immunology; D009431:Neural Conduction / Q000502:physiology; D059348:Peripheral Nerve Injuries / Q000601:surgery*; D010749:Phosphoprotein Phosphatases / Q000276:immunology; D011091:Polyesters / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000601:surgery; D033581:Stem Cell Transplantation / Q000379:methods*; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; C577486:Nes protein, rat; D064231:Nestin; D011091:Polyesters; C528900:glial fibrillary astrocytic protein, mouse; C016240:polycaprolactone; C049301:microtubule-associated protein-2 phosphatase; D010749:Phosphoprotein Phosphatases
keywords: Adipose Tissue; Matrix Attachment Regions; Nanotubes; Peripheral Nerves; Polycaprolactone; Regeneration; Stem Cells