title: [Effects of Xiaoyao Powder Extract in co-culture model of melanoma cells and keratinocytes on expression of tyrosinase and its related protein].
abstract: OBJECTIVE
  To observe the effects and mechanism of Xiaoyao Powder extract on the content of melanin in co-culture model of melanoma cells and keratinocytes.
  
  
  METHODS
  Eluting components of Xiaoyao Powder was collected by AB-8 macroporous resin column. Different concentration extracts of Xiaoyao Powder were added into the co-culture model of A375 melanoma cells and HaCat ke- ratinocytes. Hunt method was used to detected the content of melanin. RT-PCR assay was used to detect the effects of the extract of Xi- aoyao Powder on the TYR,TRP-1 and TRP-2 mRNA expression in A375 melanoma cells.
  
  
  RESULTS
  Compared with the control group,the extract of Xiaoyao Powder down-regulated content of melanin and mRNA expression of TYR,TRP-1 and TRP-2 in A375 melanoma cells by 82.23% ,93. 01% and 29. 11% ,23.78% ,20. 05% ;25. 13% ,15.02% ,11.64% ,respectively(P < 0. 01).
  
  
  CONCLUSIONS
  The extract of Xiaoyao Powder can decrease the melanogenesis by down-regulating the mRNA expression of tyrosinase and its related protein.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Ming-lei
authors.initials: ML
authors.lastname: Zhang
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yan-dong
authors.initials: YD
authors.lastname: Ren
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Jian-min
authors.initials: JM
authors.lastname: Li
mesh_terms: D018920:Coculture Techniques; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007370:Interferon Type I; D019746:Intramolecular Oxidoreductases; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism*; D008543:Melanins; D008545:Melanoma / Q000201:enzymology*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D011208:Powders; D011257:Pregnancy Proteins
chemical_list: D004365:Drugs, Chinese Herbal; D007370:Interferon Type I; D008543:Melanins; D011208:Powders; D011257:Pregnancy Proteins; C020947:interferon tau; C516449:xiaoyao; D010088:Oxidoreductases; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase