title: Pinctada fucata mantle gene 4 (PFMG4) from pearl oyster mantle enhances osteoblast differentiation.
abstract: The organic matrix of nacre has been reported for its effect on osteogenesis. It was found that PFMG4 (Pinctada fucata mantle gene 4) with an N-terminal signal peptide could be secreted into nacre of Pinctada fucata (P. fucata). Here, we report that PFMG4 is highly expressed in mantle tissue and has high homology with C1q protein in different species. In MC3T3-E1 osteoblast cells, we found that highly expressed PFMG4 could suppress cell proliferation and type I collagen expression, but it could increase alkaline phosphatase activity and mineralized deposition. These results show that PFMG4 has potential ability in enhancing osteoblast differentiation, suggesting a new idea in developing medicine for the therapy of osteoporosis.
authors.affiliation: a Protein Science Key Laboratory of the Ministry of Education, School of Medicine , Tsinghua University , Beijing , P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Kenichi
authors.initials: K
authors.lastname: Harimoto
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fuji
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Liyuan
authors.initials: L
authors.lastname: Li
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001685:Biological Factors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D015922:Complement C1q / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D049911:Pinctada / Q000737:chemistry* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D001685:Biological Factors; D024042:Collagen Type I; D015922:Complement C1q; D000469:Alkaline Phosphatase
keywords: MC3T3-E1; Pinctada fucata; Pinctada fucata mantle gene 4 (PFMG4); biomineralization