title: Biological response of human mesenchymal stromal cells to titanium grade 4 implants coated with PCL/ZrO₂ hybrid materials synthesized by sol-gel route: in vitro evaluation.
abstract: The surface modification of implantable materials in order to improve their biological proprieties, including tissue tolerance and osseointegration ability, by means of functional coating deposition is a promising strategy to provide a firm fixation of the implants. In this study, organic/inorganic hybrid materials consisting of an inorganic zirconia-based matrix, in which a biocompatible polymer, poly(ε-caprolactone) (PCL), has been incorporated at different percentages, have been synthesized via sol-gel route. Developed materials have been used to coat titanium grade 4 substrates by means of dip coating technique. Scanning electron microscopy (SEM) analysis of the obtained coatings has shown that films crack-free can be obtained for high levels of PCL. Chemical composition and interactions between organic and inorganic moieties have been studied by Attenuated Total Reflectance Fourier Transform InfraRed spectroscopy. The bone-bonding capability of the nanocomposite films has been evaluated in vitro by examining the appearance of an apatite layer on their surface when soaked in a simulated body fluid by means of SEM equipped with EDS microanalysis. In vitro biocompatibility assessment was performed in combination with human mesenchymal stromal cells (hMSCs). Materials were found to be non-toxic and supporting cell proliferation. Additionally, the coating material was not hampering the differentiation of hMSCs in an osteogenic medium.
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa, Italy. Electronic address: michelina.catauro@unina2.it.
authors.forename: M
authors.initials: M
authors.lastname: Catauro
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bollino
authors.affiliation: Department of Industrial and Information Engineering, Second University of Naples, Via Roma 29, 81031 Aversa, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Papale
authors.affiliation: Tissue Engineering Lab, Center for Integrated Research, "Università Campus Bio-Medico di Roma", via Alvaro del Portillo, 00128 Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Mozetic
authors.affiliation: Tissue Engineering Lab, Center for Integrated Research, "Università Campus Bio-Medico di Roma", via Alvaro del Portillo, 00128 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rainer
authors.affiliation: Tissue Engineering Lab, Center for Integrated Research, "Università Campus Bio-Medico di Roma", via Alvaro del Portillo, 00128 Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Trombetta
mesh_terms: D001031:Apatites / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D019736:Prostheses and Implants; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology; D015040:Zirconium
chemical_list: D001031:Apatites; D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D019904:Polymethyl Methacrylate; D015040:Zirconium; D014025:Titanium; C028541:zirconium oxide
keywords: Biological response; Human mesenchymal stromal cells; Organic/inorganic hybrid material; Sol–gel; Titanium