title: Local delivery of mesenchymal stem cells with poly-lactic-co-glycolic acid nano-fiber scaffold suppress arthritis in rats.
abstract: Mesenchymal stem cells (MSC) have been used recently for the treatment of autoimmune diseases in murine animal models due to the immunoregulatory capacity. Current utilization of MSC requires cells in certain quantity with multiple courses of administration, leading to limitation in clinical usage. Here we efficiently treated collagen-induced arthritis rats with a single local implantation with reduced number of MSC (2∼20% of previous studies) with nano-fiber poly-lactic-co-glycolic acid (nano-fiber) scaffold. MSC seeded on nano-fiber scaffold suppressed arthritis and bone destruction due to inhibition of systemic inflammatory reaction and immune response by suppressing T cell proliferation and reducing anti- type II collagen antibody production. In vivo tracing of MSC demonstrated that these cells remained within the scaffold without migrating to other organs. Meanwhile, in vitro culture of MSC with nano-fiber scaffold significantly increased TGF-β1 production. These results indicate an efficient utilization of MSC with the scaffold for destructive joints in rheumatoid arthritis by a single and local inoculation. Thus, our data may serve as a new strategy for MSC-based therapy in inflammatory diseases and an alternative delivery method for bone destruction treatment.
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Yamaoka
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Koshiro
authors.initials: K
authors.lastname: Sonomoto
authors.affiliation: Integrative Technology Research Institute, Teijin Limited, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kaneko
authors.affiliation: Integrative Technology Research Institute, Teijin Limited, Tokyo, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Satake
authors.affiliation: Integrative Technology Research Institute, Teijin Limited, Tokyo, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan; Pharmacology Research Laboratories I, Research Division, Mitsubishi Tanabe Pharma Corporation, Yokohama, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kondo
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Jidong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Ippei
authors.initials: I
authors.lastname: Miyagawa
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Yamagata
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Fukuyo
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Okada
authors.affiliation: The First Department of Internal Medicine, University of Occupational and Environmental Health, Japan, Kitakyushu, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Tanaka
mesh_terms: D000328:Adult; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology / Q000628:therapy*; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters; D011100:Polyglycolic Acid / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011917:Rats, Inbred Lew; D054457:Tissue Scaffolds / Q000737:chemistry; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; D011091:Polyesters; D011108:Polymers; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D011100:Polyglycolic Acid; D019344:Lactic Acid; C033616:poly(lactide)