title: Proteins involved in flor yeast carbon metabolism under biofilm formation conditions.
abstract: A lack of sugars during the production of biologically aged wines after fermentation of grape must causes flor yeasts to metabolize other carbon molecules formed during fermentation (ethanol and glycerol, mainly). In this work, a proteome analysis involving OFFGEL fractionation prior to LC/MS detection was used to elucidate the carbon metabolism of a flor yeast strain under biofilm formation conditions (BFC). The results were compared with those obtained under non-biofilm formation conditions (NBFC). Proteins associated to processes such as non-fermentable carbon uptake, the glyoxylate and TCA cycles, cellular respiration and inositol metabolism were detected at higher concentrations under BFC than under the reference conditions (NBFC). This study constitutes the first attempt at identifying the flor yeast proteins responsible for the peculiar sensory profile of biologically aged wines. A better metabolic knowledge of flor yeasts might facilitate the development of effective strategies for improved production of these special wines.
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, Severo Ochoa Building, Ctra. N-IV-A km 396, 14014 Cordoba, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Moreno-García
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, Severo Ochoa Building, Ctra. N-IV-A km 396, 14014 Cordoba, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: García-Martínez
authors.affiliation: Department of Agricultural Chemistry, Agrifood Campus of International Excellence ceiA3, University of Cordoba, Marie Curie Building, Ctra. N-IV-A km 396, 14014 Cordoba, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Moreno
authors.affiliation: Department of Microbiology, Agrifood Campus of International Excellence ceiA3, University of Cordoba, Severo Ochoa Building, Ctra. N-IV-A km 396, 14014 Cordoba, Spain. Electronic address: mi1gamaj@uco.es.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Mauricio
mesh_terms: D018441:Biofilms / Q000254:growth & development; D002244:Carbon / Q000378:metabolism*; D005285:Fermentation; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon
keywords: Biofilm; Carbon metabolism; Flor yeast; OFFGEL; Proteins