title: Tectorigenin inhibits the inflammation of LPS-induced acute lung injury in mice.
abstract: OBJECTIVE
  In a previous study, the anti-inflammatory effects of tectorigenin were disclosed. In this study, the anti-inflammatory effects of tectorigenin on acute lung injury using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model were investigated
  
  
  METHODS
  The cell-count in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by the wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed using SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α), IL-1β, and IL-6 were assayed using an enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed through HE staining. The inflammatory signal pathway related protein nuclear factor NF-κB p65 mRNA expression was measured by real-time PCR, and the protein level of NF-κB p65 was measured using Western blotting analysis.
  
  
  RESULTS
  The data showed that treatment with the tectorigenin markedly attenuated the inflammatory cell numbers in the BALF, decreased nuclear factor NF-κB p65 mRNA level and protein level in the lungs, and improved SOD activity and inhibited MPO activity. Histological studies showed that tectorigenin substantially inhibited LPS-induced neutrophils in lung tissue compared with the model group.
  
  
  CONCLUSIONS
  The results indicated that tectorigenin had a protective effect on LPS-induced ALI in mice.
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China; Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Ma
authors.affiliation: Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Liu
authors.affiliation: Discipline of Chinese and Western Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing 210046, China. Electronic address: qur2004@yahoo.com.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Qu
authors.affiliation: Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. Electronic address: spma@cpu.edu.com.
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Ma
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D005260:Female; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D007529:Isoflavones / Q000627:therapeutic use*; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000032:analysis; D011654:Pulmonary Edema / Q000473:pathology; D013482:Superoxide Dismutase / Q000032:analysis
chemical_list: D007529:Isoflavones; D008070:Lipopolysaccharides; C120039:tectorigenin; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: Inflammation; LPS-induced acute lung injury; Tectorigenin