title: [LC3-LpqH enhances the immunoprotection of Ag85B DNA vaccine against Mycobacterium tuberculosis].
abstract: OBJECTIVE
  To explore the effect of microtubule-associated protein light chain 3- lipoprotein antigen precursor (pCMV-LC3-LpqH) as an DNA adjuvant on the immunoprotection of the anti-tuberculosis DNA vaccine encoding Ag85B antigen from Mycobacterium tuberculosis (MTB).
  
  
  METHODS
  BALB/c mice were randomly divided into four groups that were immunized three times with pCMV-Ag85B, pCMV-LpqH/pCMV-Ag85B, pCMV-LC3-LpqH/pCMV-Ag85B and pCMV, respectively. Two weeks after the final vaccination, the titers and subtypes of anti-Ag85B IgG of BALB/c mice were detected by ELISA. In vitro, the levels of interleukin 2 (IL-2), interferon γ (IFN-γ), IL-4 and IL-10 that were secreted from Ag85B-stimulated splenocytes were also measured by ELISA. Three months after the final vaccination, mice were challenged with virulent MTB H37Rv strain through the tail veins. The mice spleens and lungs were dissected out for counting colony-forming unit (CFU) and observing pulmonary histopathological changes after MTB infection.
  
  
  RESULTS
  LC3-LpqH/Ag85B-immunized mice showed the significant increase of IgG2a, IL-2 and IFN-γ, along with the decrease of IgG1, IL-4 and IL-10, lower MTB CFU in the spleens and lungs and a marked relief in pulmonary injury.
  
  
  CONCLUSIONS
  LC3-LpqH could enhance Ag85B-specific Th1 immune response that exerts a superior immune protection, which might be a new practical strategy for developing MTB vaccines.
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hu
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Xiaokang
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Gong
authors.initials: G
authors.lastname: Chen
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Dai
authors.affiliation: Department of Pathology and Histology, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Chen
authors.affiliation: Institute of Infection and Immunology, Department of Immunology and Laboratory Medicine, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Zhang
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D008074:Lipoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008869:Microtubule-Associated Proteins / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: C075966:Ag38 antigen, Mycobacterium tuberculosis; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D008074:Lipoproteins; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis