title: Proteomic analysis of rat plasma with experimental autoimmune uveitis based on label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS).
abstract: Uveitis is a severe autoimmune eye disease that can cause intraocular inflammation even lead to severe vision loss, and the occurrence of uveitis can be closely associated with abnormal expression of proteins. However, the abnormally expressed proteins involved in uveitis are not well identified. Using liquid chromatography-tandem mass spectrometry technique, we examined the alterations in proteomic expression profiling in rat plasma specimens related to experimental autoimmune uveitis (EAU) versus normal samples. In addition, the experimental verification was further performed using enzyme-linked immunosorbent assay (ELISA) for abnormally expressed proteins in EAU rat plasma. The results indicate that 62 proteins were upregulated and 106 proteins were downregulated in plasma from EAU rats compared with those in saline-treated samples. In the meantime, we observed that the plasma level of complement component 3 in EAU rats was upregulated versus saline-treated rats (from 92.32μg/mL to 168.92μg/mL), whereas the level of interleukin-1 receptor accessory protein was downregulated (from 1120.97pg/mL to 798.39pg/mL), and these results were highly in agreement with those of mass spectrometry determination. Taken together, our results indicate that liquid chromatography-tandem mass spectrometry analysis possesses a good resolution for peptides in plasma, and the findings will provide the baseline plasma dataset for EAU rats and the relevant information can contribute to future studies on the understanding the mechanism of uveitis.
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, China; Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, China; Eye Institute of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, China.
authors.forename: Dadong
authors.initials: D
authors.lastname: Guo
authors.affiliation: Thermo Fisher Scientific (Shanghai), Building 6, No. 27, Xin Jinqiao Road, Shanghai 201206, China.
authors.forename: Peiming
authors.initials: P
authors.lastname: Gu
authors.affiliation: Shandong University of Traditional Chinese Medicine, China.
authors.forename: Zhengfeng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Shandong University of Traditional Chinese Medicine, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Tang
authors.affiliation: Shandong University of Traditional Chinese Medicine, China.
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Du
authors.affiliation: Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, China; Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, China; Eye Institute of Shandong University of Traditional Chinese Medicine, No. 48#, Yingxiongshan Road, Jinan 250002, China. Electronic address: azuresky1999@163.com.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Bi
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005260:Female; D040901:Proteomics / Q000379:methods*; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014605:Uveitis / Q000097:blood*
keywords: Experimental autoimmune uveitis; Mass spectrometry; Plasma; Proteomics; Rat