title: Dynamics of membrane fatty acid composition of succinic acid-producing Anaerobiospirillum succiniciproducens.
abstract: The dynamics of the membrane fatty acid composition of a succinic acid-producing bacterium, Anaerobiospirillum succiniciproducens, was studied using continuous and batch cultures. In both batch and chemostat continuous culture conditions, A. succiniciproducens grown on glucose or glycerol responded to acid stress by lowering the content of saturated fatty acids (SFAs) and increasing the content of unsaturated fatty acids (UFAs). Notably, A. succiniciproducens elongated C16:0 (SFA) and converted it to C18:1 (UFA) in the pH-uncontrolled culture, which is a common bacterial adaptation strategy to combat acid stress. Interestingly, A. succiniciproducens did not exhibit a significant increase in cyclic FA content, which is another common strategy to cope with acidic conditions.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, South Korea.
authors.forename: Seung Phill
authors.initials: SP
authors.lastname: Choi
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, South Korea.
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju 500-757, South Korea.
authors.forename: Joing-il
authors.initials: JI
authors.lastname: Choi
authors.affiliation: Division of Life Sciences, Korea Polar Research Institute, Incheon 406-840, South Korea.
authors.forename: Se Jong
authors.initials: SJ
authors.lastname: Han
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 443-749, South Korea. Electronic address: pclee@ajou.ac.kr.
authors.forename: Pyung Cheon
authors.initials: PC
authors.lastname: Lee
mesh_terms: D044064:Anaerobiospirillum / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D002462:Cell Membrane / Q000737:chemistry*; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019802:Succinic Acid / Q000032:analysis / Q000378:metabolism*
chemical_list: D003470:Culture Media; D005227:Fatty Acids; D019802:Succinic Acid; D005947:Glucose; D005990:Glycerol
keywords: Acid stress; Anaerobiospirillum succiniciproducens; Membrane fatty acid composition; Succinic acid