title: Development of microsatellite markers in Caryophyllaeus laticeps (Cestoda: Caryophyllidea), monozoic fish tapeworm, using next-generation sequencing approach.
abstract: Caryophyllaeus laticeps (Pallas 1781) (Cestoda: Caryophyllidea) is a monozoic tapeworm of cyprinid fishes with a distribution area that includes Europe, most of the Palaearctic Asia and northern Africa. Broad geographic distribution, wide range of definitive fish hosts and recently revealed high morphological plasticity of the parasite, which is not in an agreement with molecular findings, make this species to be an interesting model for population biology studies. Microsatellites (short tandem repeat (STR) markers), as predominant markers for population genetics, were designed for C. laticeps using a next-generation sequencing (NGS) approach. Out of 165 marker candidates, 61 yielded PCR products of the expected size and in 25 of the candidates a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for heterozygosity, Hardy-Weinberg equilibrium and the presence of null alleles on 59 individuals coming from three geographically widely separated populations (Slovakia, Russia and UK). The number of alleles in particular loci and populations ranged from two to five. Significant deficit of heterozygotes and the presence of null alleles were found in one locus in all three populations. Other loci showed deviations from Hardy-Weinberg equilibrium and the presence of null alleles only in some populations. In spite of relatively low polymorphism and the potential presence of null alleles, newly developed microsatellites may be applied as suitable markers in population genetic studies of C. laticeps.
authors.affiliation: Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001, Košice, Slovakia, hromadova@saske.sk.
authors.forename: Ivica
authors.initials: I
authors.lastname: Králová-Hromadová
authors.forename: Gabriel
authors.initials: G
authors.lastname: Minárik
authors.forename: Eva
authors.initials: E
authors.lastname: Bazsalovicsová
authors.forename: Peter
authors.initials: P
authors.lastname: Mikulíček
authors.forename: Alexandra
authors.initials: A
authors.lastname: Oravcová
authors.forename: Lenka
authors.initials: L
authors.lastname: Pálková
authors.forename: Vladimíra
authors.initials: V
authors.lastname: Hanzelová
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002589:Cestoda / Q000235:genetics* / Q000302:isolation & purification; D002590:Cestode Infections / Q000453:epidemiology / Q000469:parasitology*; D003530:Cyprinidae / Q000469:parasitology*; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology / Q000469:parasitology*; D005828:Genetics, Population; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary*; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D018154:Slovakia / Q000453:epidemiology; D006113:United Kingdom / Q000453:epidemiology
chemical_list: D018509:DNA, Helminth