title: Hyaluronic acid effect on adipose-derived stem cells. Biological in vitro evaluation.
abstract: OBJECTIVE
  To evaluate the in vitro effects of hyaluronic acid (HA) on adipose-derived stem cells (ASC) in order to consider the possibility of their combined used in the treatment of knee arthrosis.
  
  
  METHODS
  The ASC cells were grown both in the presence and absence of AH, and several studies were carried out: proliferation (WST8) and cell viability studies (Alamar Blue® and Trypan Blue), possible chondrogenic differentiation (collagen type 2 expression) by RT-PCR, AH receptor expression (CD44) by flow cytometry and RT-QPCR, and expression of inflammatory and anti-inflammatory factors (IL-6, TGFß, IL-10) by RT-QPCR.
  
  
  RESULTS
  The number of ASC significantly increased after 7 days with HA (158±39%, p <0.05). Additionally, the cell viability of the ASC treated with HA after 1, 3, 5 and 7 days was similar to that of the control cells, being considered non-toxic. There were no changes observed in the expression of CD44 and chondrogenic differentiation. TGFß expression was not modified after AH treatment, but there was a 4-fold decrease in IL-6 expression and IL-10 expression increased up to 2-fold compared to control cells.
  
  
  CONCLUSIONS
  Hyaluronic acid favours ASC proliferation without causing cellular toxicity, and inducing an anti-inflammatory profile in these cells. Hyaluronic acid appears to be a suitable vehicle for the intra-articular administration of mesenchymal stem cells.
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Unidad de Rodilla, Hospital Universitario La Paz, Madrid, España. Electronic address: alonso.moreno.garcia@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Moreno
authors.affiliation: Laboratorio de Terapia Celular, Instituto de Investigación, Hospital Universitario La Paz, Madrid, España.
authors.forename: A
authors.initials: A
authors.lastname: Martínez
authors.affiliation: Laboratorio de Terapia Celular, Instituto de Investigación, Hospital Universitario La Paz, Madrid, España.
authors.forename: S
authors.initials: S
authors.lastname: Olmedillas
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Unidad de Rodilla, Hospital Universitario La Paz, Madrid, España.
authors.forename: S
authors.initials: S
authors.lastname: Bello
authors.affiliation: Laboratorio de Terapia Celular, Instituto de Investigación, Hospital Universitario La Paz, Madrid, España; Unidad de Biotecnología Celular, Instituto de Investigación en Enfermedades Raras, Instituto de Salud Carlos III, Majadahonda, España.
authors.forename: F
authors.initials: F
authors.lastname: de Miguel
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D055675:Viscosupplements / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D055675:Viscosupplements; D006820:Hyaluronic Acid
keywords: Biocompatibilidad; Cell therapy; Células madre mesenquimales; Hyaluronic acid; Ingeniería tisular; Mesenchymal stem cells; Terapia celular; Tissular engineering ;Biocompatibility; Ácido hialurónico