title: Improved blastocyst formation with reduced culture volume: comparison of three different culture conditions on 1128 sibling human zygotes.
abstract: OBJECTIVE
  The aim of the present randomized, comparative study was to evaluate the effect of reduced culture volumes on sibling human embryo development.
  
  
  METHODS
  Firstly, sibling injected oocytes obtained from 88 out of 165 consenting couples undergoing infertility treatment were cultured either in large (35 μl) or in small drops (15 μl) of culture medium. Secondly, sibling injected oocytes from 77 couples were cultured either in large (35 μl) or in mini drops (7 μl). Embryo quality on day-2 and day-3 and blastocyst formation rate on day-5 were evaluated.
  
  
  RESULTS
  No statistically significant difference in terms of embryo quality was detected comparing embryos cultured either in large (35 μl) or small (15 μl) drops until blastocyst stage. Similarly, no difference appeared between large (35 μl) or mini (7 μl) drops until day-3, however a significantly higher blastocyst formation rate was observed in mini (7 μl) drops on day-5.
  
  
  CONCLUSIONS
  Reduced culture volume seems not to influence early embryo development but a reduction of medium appears to positively affect blastocyst development. This supports the hypothesis that the pre-implantation embryo produces autocrine factors which exert a positive effect on embryo development when culture is performed in a reduced volume.
authors.affiliation: Centre For Reproductive Medicine, European Hospital, Via Portuense 700, 00149, Rome, Italy, mg.minasi@gmail.com.
authors.forename: Maria Giulia
authors.initials: MG
authors.lastname: Minasi
authors.forename: Gemma
authors.initials: G
authors.lastname: Fabozzi
authors.forename: Valentina
authors.initials: V
authors.lastname: Casciani
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Lobascio
authors.forename: Alessandro
authors.initials: A
authors.lastname: Colasante
authors.forename: Filomena
authors.initials: F
authors.lastname: Scarselli
authors.forename: Ermanno
authors.initials: E
authors.lastname: Greco
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D009865:Oocytes / Q000502:physiology
chemical_list: D003470:Culture Media