title: Cochlioquinone derivatives with apoptosis-inducing effects on HCT116 colon cancer cells from the phytopathogenic fungus Bipolaris luttrellii L439.
abstract: A new cochlioquinone derivative, cochlioquinone F (1), as well as three known compounds, anhydrocochlioquinone A (2), isocochlioquinone A (3), and isocochlioquinone C (4), were isolated from the PDB (potato dextrose broth) culture of the phytopathogenic fungus Bipolaris luttrellii. The structure of 1 was elucidated on the basis of NMR techniques. The apoptosis-inducing effects of compounds 1-4 were evaluated against HCT116 cancer cells. Compound 2 exhibited the strongest activity in inducing apoptosis on HCT116 cells within the range of 10-30 μM. In addition, the caspase activation, the release of cytochrome c from mitochondria, and the downregulation of Bcl-2 protein in HCT116 cells treated with compound 2 were detected.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing 100101, P. R. China, (phone/fax: +86-10-64806074); University of Chinese Academy of Sciences, Yu Quan Lu 19, Shijingshan District, Beijing 100049, P. R. China.
authors.forename: Qiu-Yue
authors.initials: QY
authors.lastname: Qi
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: He
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Han
authors.forename: Quan
authors.initials: Q
authors.lastname: Chen
authors.forename: Lei
authors.initials: L
authors.lastname: Cai
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001203:Ascomycota / Q000737:chemistry*; D016227:Benzoquinones / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D016227:Benzoquinones; C066134:cochlioquinone A
keywords: Apoptosis-inducing effect; Bipolaris luttrellii; Cochlioquinone derivatives