title: Roxithromycin suppresses airway remodeling and modulates the expression of caveolin-1 and phospho-p42/p44MAPK in asthmatic rats.
abstract: Roxithromycin (RXM) expresses anti-asthmatic effects that are separate from its antibiotic activity, but its effects on airway remodeling are still unknown. Here, we evaluated the effects of RXM on airway remodeling and the expression of caveolin-1 and phospho-p42/p44mitogen-activated protein kinase (phospho-p42/p44MAPK) in chronic asthmatic rats. The chronic asthma was induced by ovalbumin/Al(OH)3 sensitization and ovalbumin challenge, RXM (30mg/kg) or dexamethasone (0.5mg/kg) was given before airway challenge initiation. We measured the thickness of bronchial wall and bronchial smooth muscle cell layer to indicate airway remodeling, and caveolin-1 and phospho-p42/p44MAPK expression in lung tissue and airway smooth muscle were detected by immunohistochemistry and western blot analysis, respectively. The results demonstrated that RXM treatment decreased the thickness of bronchial wall and bronchial smooth muscle cell layer, and also downregulated the phospho-p42/p44MAPK expression and upregulated the caveolin-1 expression. The above effects of RXM were similar to dexamethasone. Our results suggested that pretreatment with RXM could suppress airway remodeling and regulate the expression of caveolin-1 and phospho-p42/p44MAPK in chronic asthmatic rats.
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Critical Care, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Wang
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China. Electronic address: 565425732@qq.com.
authors.forename: Yuan-Rong
authors.initials: YR
authors.lastname: Dai
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Feng-Qin
authors.initials: FQ
authors.lastname: Li
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Hai-Ya
authors.initials: HY
authors.lastname: Wu
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Sun-Shun
authors.initials: SS
authors.lastname: Yan
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Liang-Rong
authors.initials: LR
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Li-da
authors.initials: LD
authors.lastname: Jin
authors.affiliation: Department of Pulmonary Medicine, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Xia
mesh_terms: D056151:Airway Remodeling / Q000187:drug effects; D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000188:drug therapy*; D001980:Bronchi / Q000187:drug effects* / Q000473:pathology; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002908:Chronic Disease; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000502:physiology; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D015575:Roxithromycin / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: D000485:Allergens; D051242:Caveolin 1; D015575:Roxithromycin; D003907:Dexamethasone; D010047:Ovalbumin; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3
keywords: Airway remodeling; Asthma; Caveolin-1; Phospho-p42/p44MAPK; Rats; Roxithromycin