title: Accelerated Fmoc solid-phase synthesis of peptides with aggregation-disrupting backbones.
abstract: In this work, we describe an accelerated solid-phase synthetic protocol for ordinary or difficult peptides involving air-bath heating and amide protection. For the Hmsb-based backbone amide protection, an optimized acyl shift condition using 1,4-dioxane was discovered. The efficiency and robustness of the protocol was validated in the course of preparation of classical difficult peptides and ubiquitin protein segments.
authors.affiliation: School of Medical Engineering, Hefei University of Technology, Hefei 230009, China. ymli@hfut.edu.cn lym2007@mail.ustc.edu.cn.
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Huang
authors.forename: Chao-Jian
authors.initials: CJ
authors.lastname: Guan
authors.forename: Xiang-Long
authors.initials: XL
authors.lastname: Tan
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Chen
authors.forename: Qing-Xiang
authors.initials: QX
authors.lastname: Guo
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Li
mesh_terms: D000388:Air; D000577:Amides / Q000737:chemistry; D004146:Dioxanes / Q000737:chemistry; D005449:Fluorenes / Q000737:chemistry*; D006358:Hot Temperature; D007700:Kinetics; D010455:Peptides / Q000138:chemical synthesis*; D060327:Solid-Phase Synthesis Techniques; D012997:Solvents / Q000737:chemistry
chemical_list: C496789:9-fluorenylmethoxycarbonyl; D000577:Amides; D004146:Dioxanes; D005449:Fluorenes; D010455:Peptides; D012997:Solvents; C025223:1,4-dioxane