title: Single asparagine to arginine mutation allows PerR to switch from PerR box to fur box.
abstract: Fur family proteins, ubiquitous in prokaryotes, play a pivotal role in microbial survival and virulence in most pathogens. Metalloregulators, such as Fur and PerR, regulate the transcription of genes connected to iron homeostasis and response to oxidative stress, respectively. In Bacillus subtilis, Fur and PerR bind with high affinity to DNA sequences differing at only two nucleotides. In addition to these differences in the PerR and Fur boxes, we identify in this study a residue located on the DNA binding motif of the Fur protein that is critical to discrimination between the two close DNA sequences. Interestingly, when this residue is introduced into PerR, it lowers the affinity of PerR for its own DNA target but confers to the protein the ability to interact strongly with the Fur DNA binding sequence. The present data show how two closely related proteins have distinct biological properties just by changing a single residue.
authors.affiliation: §CNRS UMR 5249, LCBM, F-38054 Grenoble, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Caux-Thang
authors.affiliation: †Université Grenoble Alpes, LCBM, F-38054 Grenoble, France.
authors.forename: Aubérie
authors.initials: A
authors.lastname: Parent
authors.affiliation: ‡CEA, DSV, iRTSV, LCBM, PMB, F-38054 Grenoble, France.
authors.forename: Ramakrishnan
authors.initials: R
authors.lastname: Sethu
authors.affiliation: †Université Grenoble Alpes, LCBM, F-38054 Grenoble, France.
authors.forename: Arhamatoulaye
authors.initials: A
authors.lastname: Maïga
authors.affiliation: §CNRS UMR 5249, LCBM, F-38054 Grenoble, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Blondin
authors.affiliation: ‡CEA, DSV, iRTSV, LCBM, PMB, F-38054 Grenoble, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Latour
authors.affiliation: §CNRS UMR 5249, LCBM, F-38054 Grenoble, France.
authors.forename: Victor
authors.initials: V
authors.lastname: Duarte
mesh_terms: D001120:Arginine / Q000378:metabolism; D001216:Asparagine / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012097:Repressor Proteins; C068477:ferric uptake regulating proteins, bacterial; C122138:peroxide repressor proteins; D001216:Asparagine; D001120:Arginine