title: Isolation and molecular identification of mycoplasma genitalium from the secretion of genital tract in infertile male and female.
abstract: BACKGROUND
  Mycoplasmas can cause acute and chronic diseases at multiple sites with wide-range complications and have been implicated as cofactors in diseases. The infections influenced form genital mycoplasmas specifically Mycoplasma hominis and Mycoplasma genitalium potentially affect reproductive disorders, and infertility.
  
  
  OBJECTIVE
  Isolation and molecular identification of Mycoplasma genitalium from the genital tract of infertile male and vaginal discharge of infertile female referred to Infertility Center of Kerman in 2013.
  
  
  METHODS
  This study was a randomized, prospective study. We included 100 infertile male and 100 infertile female that were referred to the Infertility Center of Kerman. Then for isolation and molecular identification of Mycoplasma genitalium from urethral and vaginal discharge polymerase chain reaction was performed on Mycoplasma genus and genitalium.
  
  
  RESULTS
  From a total of 100 semen samples 45 patients (45%) were mycoplasma-positive and 13 (28.8%) were genitalium species positive. Also, from a total of 100 women samples 43 women (43%) were mycoplasma-positive and 10 (23.2%) were genitalium species positive. Positive samples were sequenced and phylogenetic tree was drawn.
  
  
  CONCLUSIONS
  According to the results of this study, a high percentage of infertile male and female were infected with the Mycoplasma genitalium. For prevention of harmful and significant consequences of this infection, we suggest a screening program in symptomatic infertile couples.
authors.affiliation: Department of IVF, Afzalipoor Hospital, IVF Center, Kerman, Iran.
authors.forename: Naeime
authors.initials: N
authors.lastname: Mohseni Moghadam
authors.affiliation: Department of Microbiology, College of Science, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Babak
authors.initials: B
authors.lastname: Kheirkhah
authors.affiliation: Department of Pathology, Afzalipoor Medical College, Kerman, Iran.
authors.forename: Toraj Reza
authors.initials: TR
authors.lastname: Mirshekari
authors.affiliation: Department of Parasitology, Afzalipoor Medical College, Kerman, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Fasihi Harandi
authors.affiliation: Department of Molecular Medical Genetic, Biotechnology Research Center, Pasteur Institute, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Tafsiri
keywords: Asymptomatic Infections; Infertility; Mycoplasma genitalium; Sequence Analysis; polymerase chain reaction