title: [Determination of anthraquinones from different medicinal parts of Berchemia lineata by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for determination of emodin,chrysophanol and physcion from different medicinal parts of Berchemia lineata.
  
  
  METHODS
  Samples were analyzed on Diamonsil ODS C18 (250 mm x 4. 6 mm,5 μm), with the mobile phase consisted of methanol-0. 20% phosphoric acid solution(74: 26). The flow rate was 1.0 mL/min,column temperature was set at 35 °C ,and detection UV wavelength was 254 nm.
  
  
  RESULTS
  The linear range of emodin, chrysophanol and physcion was 0. 00201~ 0. 0804 μg,0. 0066~0. 264 μg and 0. 0124 ~0. 496 μg,with the average recovery was 100. 43% ,101. 29% and 98. 36% ,respectively. The content of total anthraquinones in root was higher than that in taten of Berchemia lineata.
  
  
  CONCLUSIONS
  The method is simple,accurate and reliable for quality control of Berchemia lineata.
authors.forename: Ji-wen
authors.initials: JW
authors.lastname: Wang
authors.forename: Zhi-jian
authors.initials: ZJ
authors.lastname: Fang
authors.forename: Jin-le
authors.initials: JL
authors.lastname: Cheng
authors.forename: Han-jing
authors.initials: HJ
authors.lastname: Yan
mesh_terms: D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004642:Emodin / Q000031:analogs & derivatives* / Q000032:analysis*; D018517:Plant Roots; D011786:Quality Control; D027823:Rhamnaceae / Q000737:chemistry*
chemical_list: D000880:Anthraquinones; C008905:physcione; D004642:Emodin; C027113:chrysophanic acid