title: G-protein-coupled receptor 137 accelerates proliferation of urinary bladder cancer cells in vitro.
abstract: Urinary bladder cancer is a worldwide concern because of its level of incidence and recurrence. To search an effective therapeutic strategy for urinary bladder cancer, it is important to identify proteins involved in tumorigenesis that could serve as potential targets for diagnosis and treatment. G-protein-coupled receptors (GPRs) constitute a large protein family of receptors that sense molecules outside the cell and activate signal transduction pathways and cellular responses inside the cell. GPR137 is a newly discovered human gene encoding orphan GPRs. In this study, we aimed to investigate the physiological role of GPR137 in urinary bladder cancer. The effect of GPR137 on cell growth was examined via an RNA interference (RNAi) lentivirus system in two human urinary bladder cancer cell lines BT5637 and T24. Lentivirus-mediated RNAi could specifically suppressed GPR137 expression in vitro, resulting in alleviated cell viability and impaired colony formation, as well as blocks G0/G1 and S phases of the cell cycle. These results suggested GPR137 as an essential player in urinary bladder cancer cell growth, and it may serve as a potential target for gene therapy in the treatment of urinary bladder cancer.
authors.affiliation: Department of Urology, Shanghai First People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Yiheng
authors.initials: Y
authors.lastname: Du
authors.affiliation: Department of Urology, Weifang Hospital of Chinese Traditional Medicine, Shandong, People's Republic of China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Bi
authors.affiliation: Department of Urology, Shanghai First People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Urology, Shanghai First People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Urology, Shanghai First People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Shujie
authors.initials: S
authors.lastname: Xia
authors.affiliation: Department of Urology, Shanghai First People's Hospital affiliated to Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Liu
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D043562:Receptors, G-Protein-Coupled / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
chemical_list: C112315:GPR137B protein, human; D043562:Receptors, G-Protein-Coupled
keywords: G-protein-coupled receptor 137; RNA interference; lentivirus; proliferation; urinary bladder cancer