title: Genetic diversity analysis among collected purslane (Portulaca oleracea L.) accessions using ISSR markers.
abstract: Genetic diversity and relationships among 45 collected purslane accessions were evaluated using ISSR markers. The 28 primers gave a total of 167 bands, among which 163 were polymorphic (97.6%). The genetic diversity as estimated by Shannon's information index was 0.513, revealing a quite high level of genetic diversity in the germplasm. The average number of observed allele, effective allele, expected heterozygosity, polymorphic information content (PIC) and Nei's index were 5.96, 1.59, 0.43, 0.35 and 0.35, respectively. The UPGMA dendrogram based on Nei's genetic distance grouped the whole germplasm into 7 distinct clusters. The analysis of molecular variance (AMOVA) revealed that 89% of total variation occurred within population, while 11% were found among populations. Based on the constructed dendrogram using ISSR markers those accessions that are far from each other by virtue of genetic origin and diversity index (like Ac1 and Ac42; Ac19 and Ac45; Ac9 and Ac23; Ac18 and A25; Ac24 and Ac18) are strongly recommended to select as parent for future breeding program to develop high yielding and stress tolerant purslane variety in contribution to global food security.
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia. Electronic address: amirul81@gmail.com.
authors.forename: M Amirul
authors.initials: MA
authors.lastname: Alam
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia.
authors.forename: Abdul Shukor
authors.initials: AS
authors.lastname: Juraimi
authors.affiliation: Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia; Institutes of Tropical Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia.
authors.forename: Mohd Yusop
authors.initials: MY
authors.lastname: Rafii
authors.affiliation: Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia.
authors.forename: Azizah Abdul
authors.initials: AA
authors.lastname: Hamid
authors.affiliation: Institutes of Tropical Agriculture, Universiti Putra Malaysia, UPM Serdang, Selangor DE 43400, Malaysia.
authors.forename: Ibrahim Wasiu
authors.initials: IW
authors.lastname: Arolu
authors.affiliation: Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh.
authors.forename: M
authors.initials: M
authors.lastname: Abdul Latif
mesh_terms: D000483:Alleles; D000704:Analysis of Variance; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D031822:Portulaca / Q000235:genetics*
chemical_list: D005819:Genetic Markers
keywords: AMOVA; Dendrogram; Genetic diversity; ISSR markers; Purslane (Portulaca oleracea L.)