title: Large area CMOS bio-pixel array for compact high sensitive multiplex biosensing.
abstract: A novel CMOS bio-pixel array which integrates assay substrate and assay readout is demonstrated for multiplex and multireplicate detection of a triplicate of cytokines with single digit pg ml(-1) sensitivities. Uniquely designed large area bio-pixels enable individual assays to be dedicated to and addressed by single pixels. A capability to simultaneously measure a large number of targets is provided by the 128 available pixels. Chemiluminescent assays are carried out directly on the pixel surface which also detects the emitted chemiluminescent photons, facilitating a highly compact sensor and reader format. The high sensitivity of the bio-pixel array is enabled by the high refractive index of silicon based pixels. This in turn generates a strong supercritical angle luminescence response significantly increasing the efficiency of the photon collection over conventional farfield modalities.
authors.affiliation: PixinBio SAS, 100 Route de Houilleres, 13590 Meyreuil, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Sandeau
authors.forename: Cassandre
authors.initials: C
authors.lastname: Vuillaume
authors.forename: Sylvain
authors.initials: S
authors.lastname: Contié
authors.forename: Eva
authors.initials: E
authors.lastname: Grinenval
authors.forename: Federico
authors.initials: F
authors.lastname: Belloni
authors.forename: Hervé
authors.initials: H
authors.lastname: Rigneault
authors.forename: Roisin M
authors.initials: RM
authors.lastname: Owens
authors.forename: Margaret Brennan
authors.initials: MB
authors.lastname: Fournet
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation; D049449:Luminescence; D008163:Luminescent Measurements / Q000295:instrumentation; D012666:Semiconductors; D012825:Silicon / Q000737:chemistry*
chemical_list: D016207:Cytokines; D012825:Silicon