title: Physicochemical characterization and analgesic effect of inclusion complexes of essential oil from Hyptis pectinata L. Poit leaves with β-cyclodextrin.
abstract: The formation of inclusion complexes of Hyptis pectinata essential oil (EOHP), with potent activities such as anti-nociceptive, anti-inflammatory, among others, with β -cyclodextrin (β-CD), was obtained by slurry (SC) and paste procedures (PC). The gas chromatography coupled to the mass spectrometry (GC/MS) analysis demonstrated a total of 36.4% monoterpenes and 63.6% sesquiterpenes in the EOHP. The major components of EOHP were identified as (E)- caryophyllene (54.07%). The analysis of samples (PM, PC and SC) by GC/MS involved the surface and the total extracted oils. The GC/MS results suggested important differences between in SC and PC methods indicating the complexation of mono and sesquiterpenoids in different ratios. Furthermore, the thermal analysis techniques suggests the complexation, especially in SC, which show a thermogravimetry/derivative thermogravimetry (TG/DTG) peak at 140-270ºC, probably related to oil loss. Scanning electron microscopy (SEM) images showed reduction size of the samples mainly in the SC product. Additionally, EOHP/ β-CD improves pharmacological profile of EOHP alone in formalin-induced pain protocol in mice.
authors.forename: Paula dos P
authors.initials: Pdos P
authors.lastname: Menezes
authors.forename: Adriano A de S
authors.initials: AA
authors.lastname: Araujo
authors.forename: Grace Anne A
authors.initials: GA
authors.lastname: Doria
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans-Junior
authors.forename: Makson G B
authors.initials: MG
authors.lastname: de Oliveira
authors.forename: Marcio R V
authors.initials: MR
authors.lastname: dos Santos
authors.forename: Juliana F
authors.initials: JF
authors.lastname: de Oliveira
authors.forename: Jivaldo do R
authors.initials: Jdo R
authors.lastname: Matos
authors.forename: Flavio M de S
authors.initials: FM
authors.lastname: Carvalho
authors.forename: Pericles B
authors.initials: PB
authors.lastname: Alves
authors.forename: Iara L
authors.initials: IL
authors.lastname: de Matos
authors.forename: Darlisson A
authors.initials: DA
authors.lastname: dos Santos
authors.forename: Ricardo N
authors.initials: RN
authors.lastname: Marreto
authors.forename: Gabriel F
authors.initials: GF
authors.lastname: da Silva
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Address: Av. Marechal Rondon, s/n, Cidade Universitaria, 49100-000, São Cristovao, Sergipe, Brazil. paulamenezes_16@yahoo.com.br.
authors.forename: Mairim R
authors.initials: MR
authors.lastname: Serafini
mesh_terms: D000700:Analgesics / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D000818:Animals; D002152:Calorimetry, Differential Scanning; D005260:Female; D005557:Formaldehyde; D008401:Gas Chromatography-Mass Spectrometry; D031331:Hyptis; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009822:Oils, Volatile / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use*; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy*; D064209:Phytochemicals / Q000032:analysis / Q000737:chemistry / Q000627:therapeutic use; D008517:Phytotherapy; D018515:Plant Leaves; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D000700:Analgesics; D009822:Oils, Volatile; D064209:Phytochemicals; D047392:beta-Cyclodextrins; D005557:Formaldehyde; C031215:betadex