title: Switching substrate specificity of AMT/MEP/ Rh proteins.
abstract: In organisms from all kingdoms of life, ammonia and its conjugated ion ammonium are transported across membranes by proteins of the AMT/Rh family. Efficient and successful growth often depends on sufficient ammonium nutrition. The proteins mediating this transport, the so called Ammonium Transporter (AMT) or Rhesus like (Rh) proteins, share a very similar trimeric overall structure and a high sequence similarity even throughout the kingdoms. Even though structural components of the transport mechanism, like an external substrate recruitment site, an essential twin histidine pore motif, a phenylalanine gate and the hydrophobic pore are strongly conserved and have been analyzed in detail by molecular dynamic simulations and mutational studies, the substrate(s), which pass the central pores of the AMT/Rh subunits, NH4(+), NH3 + H(+), NH4(+) + H(+) or NH3, are still a matter of debate for most proteins, including the best characterized AmtB protein from Escherichia coli. The lack of a robust expression system for functional analysis has hampered proof of structural and mutational studies, although the NH3 transport function for Rh-like proteins is rarely disputed. In plant transporters belonging to the subfamily AMT1, transport is associated with electrical currents, while some plant transporters, notably of the AMT2 type, were suggested to transport NH3 across the membrane, without associated ionic currents. Here we summarize data in favor of each substrate for the distinct AMT/Rh classes, discuss mutants and how they differ in structure and functionality. A common mechanism with deprotonation and subsequent NH3 transport through the central subunit pore is suggested.
authors.affiliation: a Institute of Crop Science; Nutritional Crop Physiology ; University of Hohenheim ; Stuttgart , Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Neuhäuser
authors.forename: Marek
authors.initials: M
authors.lastname: Dynowski
authors.forename: Uwe
authors.initials: U
authors.lastname: Ludewig
mesh_terms: D000595:Amino Acid Sequence; D000641:Ammonia / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017136:Ion Transport; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D027682:Cation Transport Proteins; D010940:Plant Proteins; C088952:ammonium transporters, plant; D000641:Ammonia
keywords: ammonia/ammonium; ammonium transport; membrane transport; molecular dynamics simulations; proton transport