title: Flavonoid constituents, cytotoxic and antioxidant activities of Gleditsia triacanthos L. leaves.
abstract: Gleditsia triacanthos L. is a deciduous tree belonging to the family Fabaceae. It possesses important biological activities as anti-mutagenic, anticancer, cytotoxic and treating rheumatoid arthritis. The total ethanol extract (EtOHE) and successive extracts (petroleum ether, chloroform, ethyl acetate, and aqueous ethanol) were prepared from the leaves. Eight flavone glycosides and two flavone aglycones named vicenin-I (1), vitexin (2), isovitexin (3), orientin (4), isoorientin (5), luteolin-7-O-ß-glucopyranoside (6), luteolin-7-O-ß-galactopyranoside (7), apigenin-7-O-ß-glucopyranoside (8), luteolin (9) and apigenin (10) were isolated from the aqueous ethanol extract of G. triacanthos L. leaves. Potent cytotoxic activity of the EtOHE extract was observed against the liver (IC50 = 1.68 μg), breast (IC50 = 0.74 μg), cervix (IC50 = 1.28 μg), larynx (IC50 = 0.67 μg) and colon (IC50 = 2.50 μg) cancer cell lines. Cytotoxic activity of compounds 2, 4, 6 and 8 against, the liver, breast and colon cancer cell lines was also proved. Evaluation of the in-vivo antioxidant activity of the EtOHE and successive extracts revealed that the highest activity was exhibited by 100 mg of EtOHE (97.89% potency) as compared with vitamin E (100% potency). Compound 6 showed 91.8% free radical scavenging activity.
authors.affiliation: Pharmacognosy Dept., National Research Centre, El-Tahrir St., Dokki, 12622 Cairo, Egypt.
authors.forename: R S
authors.initials: RS
authors.lastname: Mohammed
authors.affiliation: Pharmacognosy Dept., National Research Centre, El-Tahrir St., Dokki, 12622 Cairo, Egypt.
authors.forename: A H
authors.initials: AH
authors.lastname: Abou Zeid
authors.affiliation: Pharmacognosy Dept., Faculty of Pharmacy, Cairo Univ., Kasr Al-Aini, 11562 Cairo, Egypt.
authors.forename: S S
authors.initials: SS
authors.lastname: El Hawary
authors.affiliation: Pharmacology Dept., National Research Centre, El-Tahrir St., Dokki, 12622 Cairo, Egypt.
authors.forename: A A
authors.initials: AA
authors.lastname: Sleem
authors.affiliation: Pharmacognosy Dept., National Research Centre, El-Tahrir St., Dokki, 12622 Cairo, Egypt.
authors.forename: W E
authors.initials: WE
authors.lastname: Ashour
keywords: Cancer cell lines; Cytotoxic activity; Flavone glycosides; Gleditsia triacanthos L.