title: Analysis of a draft genome sequence of Kitasatospora cheerisanensis KCTC 2395 producing bafilomycin antibiotics.
abstract: Kitasatospora cheerisanensis KCTC 2395, producing bafilomycin antibiotics belonging to plecomacrolide group, was isolated from a soil sample at Mt. Jiri, Korea. The draft genome sequence contains 8.04 Mb with 73.6% G+C content and 7,810 open reading frames. All the genes for aerial mycelium and spore formations were confirmed in this draft genome. In phylogenetic analysis of MurE proteins (UDP-N-acetylmuramyl-(L)-alanyl-(D)-glutamate:DAP ligase) in a conserved dcw (division of cell wall) locus, MurE proteins of Kitasatospora species were placed in a separate clade between MurEs of Streptomyces species incorporating (LL)-diaminopimelic acid (DAP) and MurEs of Saccharopolyspora erythraea as well as Mycobacterium tuberculosis ligating meso-DAP. From this finding, it was assumed that Kitasatospora MurEs exhibit the substrate specificity for both (LL)-DAP and meso-DAP. The bafilomycin biosynthetic gene cluster was located in the left subtelomeric region. In 71.3 kb-long gene cluster, 17 genes probably involved in the biosynthesis of bafilomycin derivatives were deduced, including 5 polyketide synthase (PKS) genes comprised of 12 PKS modules.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Republic of Korea.
authors.forename: Jae Yoon
authors.initials: JY
authors.lastname: Hwang
authors.forename: Soo Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Hye Ryeung
authors.initials: HR
authors.lastname: Oh
authors.forename: Eunju
authors.initials: E
authors.lastname: Kwon
authors.forename: Doo Hyun
authors.initials: DH
authors.lastname: Nam
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000595:Amino Acid Sequence; D000935:Antifungal Agents / Q000378:metabolism*; D001483:Base Sequence; D003960:Diaminopimelic Acid / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D018942:Macrolides / Q000378:metabolism*; D005810:Multigene Family; D025282:Mycelium / Q000254:growth & development; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013379:Substrate Specificity
chemical_list: D000935:Antifungal Agents; D018942:Macrolides; D003960:Diaminopimelic Acid; D048630:Polyketide Synthases