title: Adhesive and invasive capacities of Edwardsiella tarda isolated from South American sea lion.
abstract: Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic factors, further investigation is needed to determine whether this bacterium could play a role as primary pathogen for this and other species of pinnipeds.
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Araceli
authors.initials: A
authors.lastname: Fernández
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: María Paz
authors.initials: MP
authors.lastname: Villanueva
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Mario
authors.initials: M
authors.lastname: González
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Fernández
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Fadua
authors.initials: F
authors.lastname: Latif
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Sandra Nonier
authors.initials: SN
authors.lastname: Flores
authors.affiliation: Institute of Clinical Microbiology Universidad Austral de Chile Campus Isla TejaValdivia Chile Institute of Clinical Microbiology, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
authors.forename: Heriberto
authors.initials: H
authors.lastname: Fernández
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D020609:Edwardsiella tarda / Q000302:isolation & purification / Q000502:physiology*; D004705:Endocytosis; D004756:Enterobacteriaceae Infections / Q000382:microbiology / Q000662:veterinary*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000382:microbiology*; D006801:Humans; D046070:Sea Lions / Q000382:microbiology*; D013020:South America
keywords: Edwarsiella tarda; Otaria flavescens; adherence; invasion