title: Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-β-1,4-glucanase (Cel5B) from Bacillus halodurans.
abstract: Cellulases catalyze the hydrolysis of cellulose, the major constituent of plant biomass and the most abundant organic polymer on earth. Cellulases are modular enzymes containing catalytic domains connected, via linker sequences, to noncatalytic carbohydrate-binding modules (CBMs). A putative modular endo-β-1,4-glucanase (BhCel5B) is encoded at locus BH0603 in the genome of Bacillus halodurans. It is composed of an N-terminal glycoside hydrolase family 5 catalytic module (GH5) followed by an immunoglobulin-like module and a C-terminal family 46 CBM (BhCBM46). Here, the crystallization and preliminary X-ray diffraction analysis of the trimodular BhCel5B are reported. The crystals of BhCel5B belonged to the orthorhombic space group P2121 2 and data were processed to a resolution of 1.64 Å. A molecular-replacement solution has been found.
authors.affiliation: CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Immacolata
authors.initials: I
authors.lastname: Venditto
authors.affiliation: CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Helena
authors.initials: H
authors.lastname: Santos
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot OX11 0DE, England.
authors.forename: James
authors.initials: J
authors.lastname: Sandy
authors.affiliation: Diamond Light Source, Harwell Science and Innovation Campus, Chilton, Didcot OX11 0DE, England.
authors.forename: Juan
authors.initials: J
authors.lastname: Sanchez-Weatherby
authors.affiliation: CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Luis M A
authors.initials: LM
authors.lastname: Ferreira
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu 514-8507, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Sakka
authors.affiliation: CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Carlos M G A
authors.initials: CM
authors.lastname: Fontes
authors.affiliation: CIISA, Faculdade de Medicina Veterinaria, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisbon, Portugal.
authors.forename: Shabir
authors.initials: S
authors.lastname: Najmudin
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002480:Cellulase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004591:Electrophoresis, Polyacrylamide Gel
chemical_list: D002480:Cellulase; C090774:endoglucanase EGL1
keywords: Bacillus halodurans; endo-β-1,4-glucanase Cel5B; family 46 carbohydrate-binding module (CBM46); family 5 glycoside hydrolase (GH5); plant cell-wall degradation