title: Defining the identity of human adipose-derived mesenchymal stem cells.
abstract: Adipose-derived mesenchymal stem cells (ADMSCs) are an ideal population for regenerative medical application. Both the isolation procedure and the culturing conditions are crucial steps, since low yield can limit further cell therapies, especially when minimal adipose tissue harvests are available for cell expansion. To date, a standardized procedure encompassing both isolation sites and expansion methods is missing, thus making the choice of the most appropriate conditions for the preparation of ADMSCs controversial, especially in view of the different applications needed. In this study, we compared the effects of three different commercial media (DMEM, aMEM, and EGM2), routinely used for ADMSCs expansion, and two supplements, FBS and human platelet lysate, recently proven to be an effective alternative to prevent xenogeneic antibody transfer and immune alloresponse in the host. Notably, all the conditions resulted in being safe for ADMSCs isolation and expansion with platelet lysate supplementation giving the highest isolation and proliferation rates, together with a commitment for osteogenic lineage. Then, we proved that the high ADMSC hematopoietic supportive potential is performed through a constant and abundant secretion of both GCSF and SCF. In conclusion, this study further expands the knowledge on ADMSCs, defining their identity definition and offers potential options for in vitro protocols for clinical production, especially related to HSC expansion without use of exogenous cytokines or genetic modifications.
authors.affiliation: a Cell Factory, Unit of Cell Therapy and Cryobiology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Via F. Sforza 35, 20122 Milano, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Montelatici
authors.forename: Barbara
authors.initials: B
authors.lastname: Baluce
authors.forename: Enrico
authors.initials: E
authors.lastname: Ragni
authors.forename: Cristiana
authors.initials: C
authors.lastname: Lavazza
authors.forename: Valentina
authors.initials: V
authors.lastname: Parazzi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Mazzola
authors.forename: Giovanna
authors.initials: G
authors.lastname: Cantarella
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Brambilla
authors.forename: Rosaria
authors.initials: R
authors.lastname: Giordano
authors.forename: Lorenza
authors.initials: L
authors.lastname: Lazzari
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*
chemical_list: D003470:Culture Media
keywords: adipose tissue; cellules souches mésenchymateuses; conditions de culture; culture conditions; hematopoiesis; hématopoïèse; lysat de plaquettes; mesenchymal stem cell; platelet lysate; tissu adipeux