title: Synthesis of magnetite/graphene oxide/chitosan composite and its application for protein adsorption.
abstract: In this study, a facile and novel strategy was developed to fabricate magnetite/graphene oxide/chitosan (Fe3O4/GO/CS) composite, and the composite was used as a magnetic adsorbent for the enrichment of protein, and followed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) analysis. The phase composition, chemical structure and morphology of the composite were characterized by X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), transmission electron microscopy (TEM), scanning electronic microscope (SEM) and vibrating sample magnetometer (VSM). Protein cytochrome c was chosen as model target to evaluate the adsorptive property of Fe3O4/GO/CS. After enrichment procedure and magnetic separation, protein bounded with the material was analyzed by MALDI-TOF MS without desorption. The results indicated that Fe3O4/GO/CS composite exhibited a good adsorptive capacity for protein, and Fe3O4/GO/CS composite had a promising potential in magnetic separation research.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China. Electronic address: yensh@cnu.edu.cn.
authors.forename: Nengsheng
authors.initials: N
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Pengzhi
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, PR China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Ma
mesh_terms: D000327:Adsorption; D048271:Chitosan / Q000737:chemistry*; D045304:Cytochromes c / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D010087:Oxides; D006108:Graphite; D045304:Cytochromes c; D048271:Chitosan; D052203:Ferrosoferric Oxide
keywords: Chitosan; Composite; Graphene oxide; Magnetite; Mass spectrometry; Protein enrichment