title: A chimeric embryoid body model to study vascular morphogenesis.
abstract: Embryonic stem cell (ESC)-derived embryoid body (EB) is a unique model for studying vascular development, in that it provides a three-dimensional microenvironment that mimics an in vivo milieu. When using gene-targeting EBs to study certain defects in vascular morphogenesis, it is necessary to determine whether the defect is due to the intrinsic loss of the gene in endothelial cells (EC) or rather due to the lack of surrounding factors that would typically promote vascular development. Here we describe a chimeric EB vessel development model, in which the utilization of the PECAM-GFP reporter gene in wild-type ESCs allows for the introduction of "normal" extracellular factors formed by its parallel differentiation to the gene-deletion EC that might otherwise be devoid of these factors.
authors.affiliation: Division of Vascular Surgery, Department of Surgery, Robert Wood Johnson Medical School, Rutgers-The State University of New Jersey, New Brunswick, NJ, 08903, USA.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Qi
authors.forename: Siavash
authors.initials: S
authors.lastname: Saadat
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Alan M
authors.initials: AM
authors.lastname: Graham
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000196:embryology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D058732:Embryoid Bodies / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D020868:Gene Silencing; D018390:Gene Targeting / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D051379:Mice; D009024:Morphogenesis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000235:genetics; D014162:Transfection; D020764:cdc42 GTP-Binding Protein / Q000172:deficiency / Q000235:genetics
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D049452:Green Fluorescent Proteins; D020764:cdc42 GTP-Binding Protein