title: [Sensitivity profile of Staphylococcus spp. and Streptococcus spp. isolated from toys used in a teaching hospital playroom].
abstract: OBJECTIVE
  To evaluate the presence of microorganisms of the genus Staphylococcus and Streptococcus on toys in the playroom of a teaching hospital, as well to as analyze the antimicrobial from the isolated strains.
  
  
  METHODS
  Samples were collected from 60 toys, using wet swabs, soon after being used by the children. The samples were inoculated in enriched and selective agar for isolation and later identification of the microorganisms. Antibiogram testing was performed by agar diffusion technique.
  
  
  RESULTS
  The genus Staphylococcus was present in 87.0% (52/60) of the toys. Seventythree strains were isolated, with 29.0% (21/73) coagulase-positive and 71.0% (52/73) coagulase-negative. Among the coagulase-negative strains, 90.4% were resistant to penicillin, 65.4% to oxacillin, 28.8% to clarithromycin, 61.5% to clindamycin, and none to vancomycin. Among the coagulase-positive strains, 76.2% were resistant to penicillin, 23.8% to oxacillin, 23.8% to clarithromycin, 47.6% to clindamycin, and none to vancomycin. The genus Streptococcus was not detected in any of the evaluated toys.
  
  
  CONCLUSIONS
  Toys can be contaminated with potentially pathogenic bacteria with antimicrobial resistance, representing a possible source of nosocomial infection for patients who are already debilitated.
authors.affiliation: Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil. Electronic address: vanessa_sboretti@hotmail.com.
authors.forename: Vanessa Stolf
authors.initials: VS
authors.lastname: Boretti
authors.affiliation: Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
authors.forename: Renata Nunes
authors.initials: RN
authors.lastname: Corrêa
authors.affiliation: Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
authors.forename: Silvana Soléo Ferreira
authors.initials: SS
authors.lastname: dos Santos
authors.affiliation: Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
authors.forename: Mariella Vieira Pereira
authors.initials: MV
authors.lastname: Leão
authors.affiliation: Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
authors.forename: Célia Regina
authors.initials: CR
authors.lastname: Gonçalves e Silva
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D006757:Hospital Units; D006784:Hospitals, Teaching; D008826:Microbial Sensitivity Tests; D010988:Play and Playthings; D013210:Staphylococcus / Q000187:drug effects* / Q000302:isolation & purification; D013291:Streptococcus / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents
keywords: Brinquedos; Microbial sensitivity tests; Staphylococcus; Streptococcus; Testes de sensitividade microbial; Toys