title: Mercury methylation coupled to iron reduction by dissimilatory iron-reducing bacteria.
abstract: Iron reduction and mercury methylation by dissimilatory iron-reducing bacteria (DIRB), Geobacter sulfurreducens and Shewanella oneidensis, were studied, and the relationship of mercury methylation coupled to iron reduction was determined. The ability of both bacteria for reducing iron was tested, and Fe(III) reduction occurred with the highest rate when ferric oxyhydroxide was used as a terminal electron acceptor. G. sulfurreducens had proven to mediate the production of methylmercury (MeHg), and a notable increase of MeHg following the addition of inorganic Hg was observed. When the initial concentration of HgCl2 was 500nM, about 177.03nM of MeHg was determined at 8d after G. sulfurreducens inoculation. S. oneidensis was tested negligible for Hg methylation and only 12.06nM of MeHg was determined. Iron reduction could potentially influence Hg methylation rates. The increase in MeHg was consistent with high rate of iron reduction, indicating that Fe(III) reduction stimulated the formation of MeHg. Furthermore, the net MeHg concentration increased at low Fe(III) additions from 1.78 to 3.57mM, and then decreased when the added Fe(III) was high from 7.14 to 17.85mM. The mercury methylation rate was suppressed with high Fe(III) additions, which might have been attributable to mercury complexation and low availability.
authors.affiliation: School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China. Electronic address: youbinsi@ahau.edu.cn.
authors.forename: Youbin
authors.initials: Y
authors.lastname: Si
authors.affiliation: School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.affiliation: School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Xiongyuan
authors.initials: X
authors.lastname: Si
authors.affiliation: Department of Chemistry and Biochemistry, Old Dominion University, Norfolk, VA 23529, USA.
authors.forename: Jingdong
authors.initials: J
authors.lastname: Mao
mesh_terms: D045302:Geobacter / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D008627:Mercuric Chloride / Q000378:metabolism; D008628:Mercury / Q000378:metabolism*; D008745:Methylation; D008767:Methylmercury Compounds / Q000378:metabolism*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000378:metabolism*
chemical_list: D008767:Methylmercury Compounds; D008627:Mercuric Chloride; D007501:Iron; D008628:Mercury
keywords: Dissimilatory iron-reducing bacteria; Iron reduction; Mercury methylation; Methylmercury