title: Antiviral interferon-beta signaling induced by designed transcription activator-like effectors (TALE).
abstract: Here we show that designed transcription activator-like effectors (TALEs) that bind to defined areas of the interferon beta promoter are capable to induce IFN-beta expression and signaling in human cells. Importantly, TALE-mediated IFN-beta signaling occurs independently of pathogen pattern recognition but effectively prohibits viral RNA replication as demonstrated with a hepatitis C virus replicon. TALEs were thus indicated to be valuable tools in various applications addressing, for example, virus-host interactions.
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Renner
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Nancy
authors.initials: N
authors.lastname: Funk
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Rene
authors.initials: R
authors.lastname: Geissler
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Friedrich
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Anika
authors.initials: A
authors.lastname: Penzel
authors.affiliation: Institute of Biochemistry and Biotechnology, Section Microbial Biotechnology, Martin Luther University Halle-Wittenberg, Faculty of Life Sciences (NFI), Kurt-Mothes-Str. 3, D-06120, Halle/Saale, Germany.
authors.forename: Sven-Erik
authors.initials: SE
authors.lastname: Behrens
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D016174:Hepacivirus / Q000502:physiology; D006801:Humans; D007113:Immunity, Innate; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D015202:Protein Engineering; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D015533:Transcriptional Activation; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D011994:Recombinant Proteins; D014157:Transcription Factors; D016899:Interferon-beta