title: Combining autologous peripheral blood mononuclear cells with fibroblast growth factor therapy along with stringent infection control leading to successful limb salvage in diabetic patient with chronic renal failure and severe toe gangrene.
abstract: Peripheral arterial disease (PAD) is a common complication of Diabetes Mellitus (DM) and often culminates in amputation of the affected foot. Pseudomonas aeruginosa infections associated with PAD are difficult to treat due to their multi-drug resistance. Herein we report a 38 year old male who reported with DM, chronic kidney disease (CKD) and rest pain of the right second toe in October 2011. He underwent percutaneous transluminal angioplasty (PTA) which was unsuccessful. The gangrene of the toes worsened and amputation of the right second toe was done. Bacteriological examination showed presence of P. aeruginosa which during the course of antibiotic therapy became multi-drug resistant. Gangrene and abscess of the foot worsened and amputation of the right third toe was performed. Then autologous peripheral blood mononuclear cell (PBMNC) therapy was performed but as infection control could not still be achieved, the fourth toe was amputated. A protocol of foot bath using carbonic water, local usage of antibiotics (Polymyxin-B), and basic fibroblast growth factor (b-FGF) spray was then employed after which the infection could be controlled and improvement in vascularity of the right foot could be observed in angiography. This combined approach after proper validation could be considered for similar cases.
authors.affiliation: Shimada General Hospital, Division of Cardiovascular Surgery, Chiba.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Osawa
authors.affiliation: Shimada General Hospital, Division of Cardiovascular Surgery, Chiba.
authors.forename: Kouan
authors.initials: K
authors.lastname: Orii
authors.affiliation: Biotherapy Institute of Japan, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Terunuma
authors.affiliation: The Mary-Yoshio Translational Hexagon (MYTH), Nichi-In Centre for Regenerative Medicine (NCRM), Chennai, India ; Yamanashi University- School of Medicine, Chuo, Japan.
authors.forename: Samuel Jk
authors.initials: SJ
authors.lastname: Abraham
keywords: Cell therapy; Diabetes mellitus (DM); Infection control; Pseudomonas aeruginosa; peripheral arterial disease (PAD)