title: [DNA demethylation at the promoter region enhances the expression of MMP-9 in ectopic endometrial stromal cells of endometriosis].
abstract: OBJECTIVE
  To investigate the role of DNA methylation in the regulation of matrix metalloproteinase 9 (MMP-9) gene expression in endometriosis.
  
  
  METHODS
  MMP-9 gene methylation status in eutopic and ectopic endometria were analyzed by methylation specific PCR. The expression of MMP-9 protein in eutopic and ectopic endometria were detected by immunohistochemistry. We prepared the primary cultured ectopic endometrial stromal cells, and then examined the gene expression and methylation status of MMP-9 after the culture cells were treated with 5-Aza-2-deoxycytidine.
  
  
  RESULTS
  The expression of MMP-9 in ectopic endometrial stromal cells was significantly higher than that in eutopic endometrium. MMP-9 methylation level was lower in ectopic endometrium. After treated with 5-Aza-dC, endometriosis cells showed elevated MMP-9 gene expression and methylation level.
  
  
  CONCLUSIONS
  DNA methylation at the promoter region of MMP-9 gene can enhance the expression of MMP-9 in ectopic endometrial stromal cells of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yuan
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Danhong
authors.initials: D
authors.lastname: Peng
authors.affiliation: Department of Anatomy and Neuroscience, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and Gynecology, Zhongda Hospital Affiliated to Southeast University, Medical School, Southeast University, Nanjing 210009, China.
authors.forename: Yunlang
authors.initials: Y
authors.lastname: Cai
mesh_terms: D019175:DNA Methylation; D004715:Endometriosis / Q000201:enzymology*; D004717:Endometrium / Q000166:cytology / Q000201:enzymology*; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D017154:Stromal Cells / Q000201:enzymology
chemical_list: D012333:RNA, Messenger; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9