title: Evaluation of a new chromogenic medium for direct enumeration of Campylobacter in poultry meat samples.
abstract: The present study was conducted to compare Campylobacter counts obtained by three selective media: modified charcoal cefoperazonedeoxycholate agar (mCCDA), Campy Food agar (CFA), and a novel agar RAPID' Campylobacter agar. Analysis of 12 artificially and 36 naturally contaminated samples indicated no significant differences in Campylobacter counts obtained with all three selective media. Lin's concordance correlation coefficient (CCC) and the Bland-Altman plot revealed a high level of agreement between Campylobacter counts when evaluating RAPID versus mCCDA and CFA plates. RAPID agar was the only medium tested that could effectively suppress the growth of the background microflora with naturally contaminated samples. Results of this study clearly indicated that RAPID agar is highly selective without loss of sensitivity for recovering Campylobacter. Results obtained are in agreement with those for other commonly used media; therefore, RAPID medium is suitable for Campylobacter enumeration in poultry meat samples.
authors.affiliation: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. tomasz.seliwiorstow@ugent.be.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Seliwiorstow
authors.affiliation: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Julie
authors.initials: J
authors.lastname: Baré
authors.affiliation: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Bavo
authors.initials: B
authors.lastname: Verhaegen
authors.affiliation: Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000 Gent, Belgium.
authors.forename: Mieke
authors.initials: M
authors.lastname: Uyttendaele
authors.affiliation: Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
authors.forename: Lieven
authors.initials: L
authors.lastname: de Zutter
mesh_terms: D000362:Agar / Q000737:chemistry; D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D011200:Poultry
chemical_list: D003470:Culture Media; D000362:Agar