title: Overexpression of the JAZ factors with mutated jas domains causes pleiotropic defects in rice spikelet development.
abstract: In a determinate meristem, such as a floral meristem, a genetically determined number of organs are produced before the meristem is terminated. In rice, iterative formation of organs during flower development with defects in meristem determinacy, classically called 'proliferation', is caused by several mutations and observed in dependence on environmental conditions. Here we report that overexpression of several JAZ proteins, key factors in jasmonate signaling, with mutations in the Jas domains causes an increase in the numbers of organs in florets, aberrant patterns of organ formation and repetitious organ production in spikelets. Our results imply that JAZ factors modulate mechanisms that regulate meristem functions during spikelet development.
authors.affiliation: a Bioscience and Biotechnology Center ; Nagoya University ; Chikusa , Nagoya , Japan.
authors.forename: Yutaro
authors.initials: Y
authors.lastname: Hori
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kurotani
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Toda
authors.forename: Tsukaho
authors.initials: T
authors.lastname: Hattori
authors.forename: Shin
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000595:Amino Acid Sequence; D035264:Flowers / Q000254:growth & development; D058685:Genetic Pleiotropy; D008969:Molecular Sequence Data; D009024:Morphogenesis; D009154:Mutation / Q000235:genetics*; D012275:Oryza / Q000033:anatomy & histology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary
chemical_list: D010940:Plant Proteins
keywords: JA, jasmonate; JAZ, Jasmonate zim domain; LSH1, Leafy hull sterile1; MJA, methyl jasmonate; RSS3, Rice salt sensitive 3; RT-PCR, reverse transcription-PCR; SAM, shoot apical meristem; bHLH, basic helix–loop–helix; environmental stress; extra glume; flower development; jasmonate; mJAZ, mutated JAZ; meristem determinacy; meristem identity; qRT-PCR, quantitative real-time RT-PCR; spikelet