title: Cytokinetic abscission is an acute G1 event.
abstract: Animal cell division ends with the cutting of the microtubule and membrane intercellular bridge connecting the 2 daughter cells. This process, known as cytokinetic abscission (abscission), is widely regarded as the last step of cytokinesis, i.e., the last step of the cell cycle. Major breakthroughs have been recently achieved, illuminating mechanistic aspects of abscission; however, the timing of abscission with respect to the mammalian cell cycle remains unclear. In this study, we carefully measured the onset and progression of abscission in dividing cells expressing a G1 reporter. We conclude that abscission commences long after cells enter the G1 phase. Affiliating abscission with G1 is beyond semantics since it essentially postulates that the last step of the cell cycle is regulated in, and probably by, the following cycle.
authors.affiliation: a The Mina and Everard Goodman Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials ; Bar-Ilan University ; Ramat-Gan , Israel.
authors.forename: Ofir
authors.initials: O
authors.lastname: Gershony
authors.forename: Tal
authors.initials: T
authors.lastname: Pe'er
authors.forename: Meirav
authors.initials: M
authors.lastname: Noach-Hirsh
authors.forename: Natalie
authors.initials: N
authors.lastname: Elia
authors.forename: Amit
authors.initials: A
authors.lastname: Tzur
mesh_terms: D016475:3T3 Cells; D000818:Animals; D018797:Cell Cycle Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D048749:Cytokinesis; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D016193:G1 Phase; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D018715:Microscopy, Video; D059008:Time-Lapse Imaging; D000097573:Red Fluorescent Protein
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C498645:Ris2 protein, mouse
keywords: APC/C; Abscission; Aurora; CHMP; Cell cycle; Cell division; Cep55; Constriction site; Cytokinesis; ESCRT III; Gas2l3; Mitosis; Plk1; Spastin