title: Identification of the glucosyltransferase that mediates direct flavone C-glucosylation in Gentiana triflora.
abstract: The major flavonoids accumulated in leaves of Japanese gentian (Gentiana triflora) were determined as isoorientin (luteolin 6-C-glucoside) and isoorientin 4'-glucoside. A cDNA (GtUF6CGT1) was isolated that encoded the UDP-glucose-dependent glucosyltransferase that is involved in C-glycosylflavone biosynthesis. The recombinant GtUF6CGT1 protein could transfer a glucose group to the C6 position of a flavone skeleton through C-linkage, using UDP-glucose as the glucosyl donor. These C-glycosylflavones also accumulated in petals. A good correlation was observed between GtUF6CGT1 expression and C-glycosylflavone accumulation in leaves and petals. GtUF6CGT1 is the first reported C-glucosyltransferase that mediates direct C-glucosylation of the flavone skeleton.
authors.affiliation: Iwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Sasaki
authors.affiliation: Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yuzo
authors.initials: Y
authors.lastname: Nishizaki
authors.affiliation: Iwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Yamada
authors.affiliation: Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka 020-8550, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Tatsuzawa
authors.affiliation: Department of Biological and Environmental Science, Graduate School of Agriculture, Shizuoka University, 836, Ohya, Suruga, Shizuoka 422-8529, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakatsuka
authors.affiliation: Iwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Iwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan. Electronic address: mnishiha@ibrc.or.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishihara
mesh_terms: D035264:Flowers / Q000201:enzymology* / Q000235:genetics; D030012:Gentiana / Q000201:enzymology* / Q000235:genetics; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D006031:Glycosylation; D047311:Luteolin / Q000235:genetics / Q000378:metabolism*; D018515:Plant Leaves / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; C057912:homoorientin; D005964:Glucosyltransferases; D047311:Luteolin
keywords: C-glycosylflavone; Gentiana triflora; Glucosyltransferase