title: Purification, crystallization and preliminary X-ray crystallographic studies of KstR2 (ketosteroid regulatory protein) from Mycobacterium tuberculosis.
abstract: KstR2 (Rv3557c) is one of two TetR-family transcriptional repressors of cholesterol metabolism in Mycobacterium tuberculosis. The ability to degrade cholesterol fully is important for pathogenesis, and therefore this repressor was expressed, purified and crystallized. Crystals of KstR2 diffracted to better than 1.9 Å resolution and belonged to space group C2, with unit-cell parameters a = 72.3, b = 90.3, c = 49.7 Å, α = γ = 90, β = 128.2°.
authors.affiliation: Laboratory of Structural Biology and Maurice Wilkins Centre for Molecular Biodiscovery, School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors.forename: Stephanie S
authors.initials: SS
authors.lastname: Dawes
authors.affiliation: Pathology and Pathogen Biology, The Royal Veterinary College, Royal College Street, London NW1 0TU, England.
authors.forename: Sharon L
authors.initials: SL
authors.lastname: Kendall
authors.affiliation: Laboratory of Structural Biology and Maurice Wilkins Centre for Molecular Biodiscovery, School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors.forename: Edward N
authors.initials: EN
authors.lastname: Baker
authors.affiliation: Laboratory of Structural Biology and Maurice Wilkins Centre for Molecular Biodiscovery, School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
authors.forename: J Shaun
authors.initials: JS
authors.lastname: Lott
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins
keywords: Mycobacterium tuberculosis; TetR family; cholesterol; steroid