title: Helicobacter pylori vacA i region polymorphism but not babA2 status associated to gastric cancer risk in northwestern Iran.
abstract: Helicobacter pylori-specific genotypes have been strongly associated with an increased risk of gastric cancer (GC). The aim of the present work was to study the associations of H. pylori virulence factors, vacA i region polymorphisms and babA2 status with GC risk in Azerbaijan patients. The DNA extracted from gastric biopsy specimens was used to access the babA2 and vacA genotypes. Overall, babA2 was present in 85.39 % (76/89) of H. pylori strains: 19 out of 24 (79.16 %) strains from GC, 16 out of 17 (94.14 %) strains from peptic ulcer disease (PUD) and 41 out of 48 (85.14 %) strains from chronic gastritis. No significant association was found between babA2 genotype and clinical outcomes (P > 0.05). i1 vacA polymorphism was detected in 46/89 (51.68 %) strains: in 21/24 (87.5 %), 6/17 (35.29 %) and 19/48 (39.58 %) patients with GC, PUD and chronic gastritis, respectively. i2 allele was detected in 43 (48.31 %) out of all 89 strains examined: 3 (14.28 %) of 24 strains from GC, 11 (64.71 %) of 17 from PUD, and 29 (60.42 %) of 48 strains from chronic gastritis. In this study, multiple linear regression analysis confirmed the strong association of i1 allele with GC (partial regression correlation 0.455 ± 0.101; P = 0). Results of multiple logistic regression analysis showed that vacA i1 genotype was significantly associated with GC compared with a control group (gastritis) (odds ratio 13.142, 95 % CI 3.116-55.430; P = 0). Findings from the measurement of H. pylori babA2 and vacA genotypes indicate a strong correlation between the vacA i1 allele and GC risk in the Azerbaijan area of Iran.
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Mottaghi
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran. safaralizadeh@tabrizu.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Safaralizadeh
authors.affiliation: Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Bonyadi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Latifi-Navid
authors.affiliation: Liver and Gastrointestinal Diseases Research Center, (LGDRC), Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Somi
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001426:Bacterial Proteins / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D016481:Helicobacter Infections / Q000208:ethnology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D007492:Iran / Q000208:ethnology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D013274:Stomach Neoplasms / Q000208:ethnology / Q000382:microbiology*
chemical_list: D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori
keywords: Gastric cancer; Helicobacter pylori; babA2; vacA i1