title: Identification of polyunsaturated fatty acid and diterpenoid biosynthesis pathways from draft genome of Aureispira sp. CCB-QB1.
abstract: The genus Aureispira consisting of two species, Aureispira marina and Aureispira maritima is an arachidonic acid-producing bacterium and produces secondary metabolites. In this study, we isolated a new Aureispira strain, Aureispira sp. CCB-QB1 from coastal area of Penang, Malaysia and the genome sequence of this strain was determined. The draft genome of this strain is composed of 185 contigs for 7,370,077 bases with 35.6% G+C content and contains 5911 protein-coding genes and 76 RNA genes. Linoleoyl-CoA desaturase, the key gene in arachidonic acid biosynthesis, is present in the genome. It was found that this strain uses mevalonate pathway for the synthesis of geranylgeranyl diphosphate (GGPP), which is precursor of diterpenoid, and novel pathway via futalosine for the synthesis of menaquinones. This is the first draft genome sequence of a member of the genus Aureispira.
authors.affiliation: Centre for Chemical Biology, Universiti Sains Malaysia, 10 Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia. Electronic address: go.furusawa@ccbusm.edu.my.
authors.forename: Go
authors.initials: G
authors.lastname: Furusawa
authors.affiliation: Centre for Chemical Biology, Universiti Sains Malaysia, 10 Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia.
authors.forename: Nyok-Sean
authors.initials: NS
authors.lastname: Lau
authors.affiliation: Centre for Chemical Biology, Universiti Sains Malaysia, 10 Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Alexander Chong
authors.initials: AC
authors.lastname: Shu-Chien
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Annette
authors.initials: A
authors.lastname: Jaya-Ram
authors.affiliation: Centre for Chemical Biology, Universiti Sains Malaysia, 10 Persiaran Bukit Jambul, 11900 Bayan Lepas, Penang, Malaysia; School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors.forename: Al-Ashraf Abdullah
authors.initials: AA
authors.lastname: Amirul
mesh_terms: D041963:Bacteroidetes / Q000235:genetics* / Q000502:physiology; D001482:Base Composition; D001483:Base Sequence; D053898:Biosynthetic Pathways / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D004224:Diterpenes / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D016680:Genome, Bacterial / Q000235:genetics*; D008296:Malaysia; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004224:Diterpenes; D005231:Fatty Acids, Unsaturated
keywords: Genomics; Polyunsaturated fatty acid; Saprospiraceae; Secondary metabolites