title: Isolation of the stable strain Labrys sp. BK-8 for L(+)-tartaric acid production.
abstract: A novel cis-epoxysuccinate hydrolase (CESH) producing strain of Labrys sp. BK-8 for production of L(+)-tartaric acid was isolated and identified. After optimization, a maximum activity of 3597 ± 151 U/g was achieved in batch culture in a 10 L fermentor. When Labrys sp. BK-8 was immobilized on κ-carrageenan, the immobilized cells showed a high conversion rate (>99%), enantioselectivity (EE > 99.5%) and storage stability (>90 d). A conversion rate of 97% was still achieved after 10 repeat batches. The CESH was stable over a broad range of temperatures (up to 45°C) and pH values (4.0-10.0). The Labrys sp. BK-8 isolate provides a new alternative with good stability for the industrial biosynthesis of L(+)-tartaric acid.
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China; Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, Zhejiang, China.
authors.forename: Wenna
authors.initials: W
authors.lastname: Bao
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China; Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, Zhejiang, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Pan
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China; Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, Zhejiang, China. Electronic address: xier1975@163.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Institute of Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang, China; Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, Zhejiang, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhang
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology / Q000302:isolation & purification* / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D018914:Cells, Immobilized / Q000201:enzymology / Q000378:metabolism; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D013644:Tartrates / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature
chemical_list: D013644:Tartrates; D006867:Hydrolases; C029768:tartaric acid
keywords: Immobilization; Isolation; Labrys sp.; cis-Epoxysuccinate hydrolase; l(+)-Tartaric acid