title: Redox and Src family kinase signaling control leukocyte wound attraction and neutrophil reverse migration.
abstract: Tissue damage induces early recruitment of neutrophils through redox-regulated Src family kinase (SFK) signaling in neutrophils. Redox-SFK signaling in epithelium is also necessary for wound resolution and tissue regeneration. How neutrophil-mediated inflammation resolves remains unclear. In this paper, we studied the interactions between macrophages and neutrophils in response to tissue damage in zebrafish and found that macrophages contact neutrophils and induce resolution via neutrophil reverse migration. We found that redox-SFK signaling through p22phox and Yes-related kinase is necessary for macrophage wound attraction and the subsequent reverse migration of neutrophils. Importantly, macrophage-specific reconstitution of p22phox revealed that macrophage redox signaling is necessary for neutrophil reverse migration. Thus, redox-SFK signaling in adjacent tissues is essential for coordinated leukocyte wound attraction and repulsion through pathways that involve contact-mediated guidance.
authors.affiliation: Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Tauzin
authors.affiliation: Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706.
authors.forename: Taylor W
authors.initials: TW
authors.lastname: Starnes
authors.affiliation: Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706 Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706.
authors.forename: Francisco Barros
authors.initials: FB
authors.lastname: Becker
authors.affiliation: Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706 Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706.
authors.forename: Pui-ying
authors.initials: PY
authors.lastname: Lam
authors.affiliation: Departments of Pediatrics and Medical Microbiology and Immunology, Microbiology Doctoral Training Program and Medical Scientist Training Program, Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706 Huttenlocher@wisc.edu.
authors.forename: Anna
authors.initials: A
authors.lastname: Huttenlocher
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000276:immunology; D002634:Chemotaxis, Leukocyte; D007700:Kinetics; D008264:Macrophages / Q000276:immunology; D019255:NADPH Oxidases / Q000378:metabolism; D020556:Neutrophil Infiltration; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000276:immunology*; D014945:Wound Healing / Q000276:immunology*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000378:metabolism*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D029961:Zebrafish Proteins; D019255:NADPH Oxidases; D019061:src-Family Kinases