title: [ABCA1 mRNA and protein levels in M-CSF-activated macrophages from patients with arterial stenosis].
abstract: ABCA1 transporter is one of the key factors defining the level of antiatherogenic HDL in plasma. It is actively involved in the removal of cholesterol from peripheral tissues by reverse cholesterol transport. However, the influence of the level of ABCA1 mRNA and the level of ABCA1 protein 1 in macrophages in atherosclerosis remains unexplored. Using real time PCR we determined ABCA1 mRNA level in macrophages cultured for 5 days with macrophage colony-stimulating factor (M-CSF). ABCA1 mRNA levels in macrophages from patients with arterial stenosis were increased when compared with the control group, P = 0.04. According to a Western blot analysis ABCA1 protein level in macrophages from patients was significantly lower than in the control group, P = 0.01. Our results suggest that the level of ABCA1 mRNA and level of ABCA1 protein in macrophages may be important factors in the development of atherosclerosis.
authors.forename: E P
authors.initials: EP
authors.lastname: Demina
authors.forename: V V
authors.initials: VV
authors.lastname: Miroshnikova
authors.forename: N V
authors.initials: NV
authors.lastname: Maĭorov
authors.forename: V V
authors.initials: VV
authors.lastname: Davydenko
authors.forename: A L
authors.initials: AL
authors.lastname: Shvartsman
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics* / Q000378:metabolism; D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport; D002784:Cholesterol / Q000378:metabolism; D023921:Coronary Stenosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008262:Macrophage Activation / Q000187:drug effects; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D002784:Cholesterol