title: The analysis of distribution of multidrug resistant Pseudomonas and Bacillus species from burn patients and burn ward environment.
abstract: BACKGROUND
  Infections caused by multidrug resistant bacteria act as a risk factor for mortality in burns patients. So keeping in view the crucial importance of reliable therapeutic decisions of multidrug resistance bacteria and role of hospital environment in bacteria colonization, our study is based on the evaluation of distribution of Pseudomonas sp. and Bacillus sp. in burn patients and burn ward environment.
  
  
  METHODS
  The present prospective analysis was conducted on the patients undergoing treatment in the Burn ward of Pt. B.D. Sharma University of Health Sciences, Rohtak, Haryana, during the period of January 2012 to March 2013. The multidrug resistant bacteria were characterized by following the CLSI guidelines. Molecular identification isolates were done by amplifying and sequencing 16S rDNA.
  
  
  RESULTS
  In our study out of 510 samples of 280 burn patients, 263 samples were observed sterile and bacterial isolates were obtained from 247 samples. In burn patients out of 247 samples 43 MDR strains, and in burn ward out of 60 samples 4 MDR strain were observed. After 16S rDNA amplification of MDR isolates the prevalent bacterium was belonged to the genus Bacillus (8 species; 26 isolates) followed by genus Pseudomonas (5 species; 17 isolates). The burn ward environment isolates were Pseudomonas aeruginosa, Pseudomonas stutzeri, Bacillus cereus and Acinetobacter baumanii.
  
  
  CONCLUSIONS
  The major finding of our study is the predominance of B. cereus followed by P. aeruginosa in burn patients of Pt. B.D. Sharma University of Health Sciences, Rohtak, Haryana. While considering the role of hospital environment, no direct role of environmental isolates was observed in transfer of bacterial infection.
authors.affiliation: Department of Genetics, M. D. University, Rohtak, 124001, Haryana, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Panghal
authors.affiliation: Department of Genetics, M. D. University, Rohtak, 124001, Haryana, India.
authors.forename: Khushboo
authors.initials: K
authors.lastname: Singh
authors.affiliation: Department of Genetics, M. D. University, Rohtak, 124001, Haryana, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Kadyan
authors.affiliation: Department of Microbiology, Pt. B.D. Sharma University of Health Sciences, Rohtak 124001, Haryana, India.
authors.forename: Uma
authors.initials: U
authors.lastname: Chaudary
authors.affiliation: Department of Genetics, M. D. University, Rohtak, 124001, Haryana, India. Electronic address: Yadav1964@rediffmail.com.
authors.forename: J P
authors.initials: JP
authors.lastname: Yadav
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D001407:Bacillus / Q000235:genetics* / Q000302:isolation & purification; D001409:Bacillus cereus / Q000235:genetics / Q000302:isolation & purification; D002053:Burn Units; D002056:Burns / Q000453:epidemiology / Q000382:microbiology* / Q000401:mortality; D015331:Cohort Studies; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology; D004275:DNA, Ribosomal / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D011446:Prospective Studies; D011549:Pseudomonas / Q000235:genetics* / Q000302:isolation & purification; D011552:Pseudomonas Infections / Q000453:epidemiology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification; D044223:Pseudomonas stutzeri / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: B. cereus; Burn patients; Burn ward environment; Multidrug resistant bacteria; P. aeruginosa