title: [The effect of modulators of SK channels on simple spike firing frequency in the discharge of the cerebellar Purkinje cells in laboratory mice].
abstract: The effect of CyPPA, a positive modulator of small conductance calcium-activated potassium channels of type 3 and 2 (SK3/SK2), and of NS309, an activator of intermediate and small conductance calcium-activated potassium channels (IK/SK), on the activity of cerebellar Purkinje cells was studied in 2-month-old male mice. The use of 1 mM of CyPPA has led to a decrease of simple spike firing frequency in the discharge of Purkinje cells by 25%, on average, during 1 h after application. At the same time, application of 100 μM of NS309 has promoted a decrease in simple spike firing frequency by 47 %, on average, during 1 h after the beginning of the action. The obtained results confirm the hypothesis that SK channels participate in regulation of simple spike firing frequency in the discharge of Purkinje cells and are responsible for restriction of signal frequency. The effect of NS309 on simple spike firing frequency was more pronounced; therefore, the IK/SK channels may be suggested to play the cardinal role in regulation of spike activity of Purkinje cells. Since increasing simple spike frequency in the discharge of Purkinje cells is observed at many disturbances of motor activity, in particular, at spinocerebellar ataxia, it can be suggested that the studied compounds or substances of similar action are of interest as potential medicinal agents.
authors.forename: P A
authors.initials: PA
authors.lastname: Egorova
authors.forename: T V
authors.initials: TV
authors.lastname: Karelina
authors.forename: O L
authors.initials: OL
authors.lastname: Vlasova
authors.forename: S M
authors.initials: SM
authors.lastname: Antonov
authors.forename: I B
authors.initials: IB
authors.lastname: Besprozvanny
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D007211:Indoles / Q000494:pharmacology; D008297:Male; D051379:Mice; D010091:Oximes / Q000494:pharmacology; D011689:Purkinje Cells / Q000378:metabolism / Q000502:physiology*; D011720:Pyrazoles / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D051640:Small-Conductance Calcium-Activated Potassium Channels / Q000819:agonists / Q000378:metabolism*
chemical_list: C493327:6,7-dichloro-1H-indole-2,3-dione 3-oxime; D007211:Indoles; D010091:Oximes; D011720:Pyrazoles; D011743:Pyrimidines; D051640:Small-Conductance Calcium-Activated Potassium Channels; C552889:cyclohexyl-(2-(3,5-dimethylpyrazol-1-yl)-6-methylpyrimidin-4-yl)amine