title: Dynamical DNA accessibility induced by chromatin remodeling and protein binding.
abstract: Chromatin remodeling factors are enzymes being able to alter locally chromatin structure at the nucleosomal level and they actively participate in the regulation of gene expression. Using simple rules for individual nucleosome motion induced by a remodeling factor, we designed simulations of the remodeling of oligomeric chromatin, in order to address quantitatively collective effects in DNA accessibility upon nucleosome mobilization. Our results suggest that accessibility profiles are inhomogeneous thanks to borders effects like protein binding. Remarkably, we show that the accessibility lifetime of DNA sequence is roughly doubled in the vicinity of borders as compared to its value in bulk regions far from the borders. These results are quantitatively interpreted as resulting from the confined diffusion of a large nucleosome depleted region.
authors.affiliation: Matière et Systèmes Complexes, Université Paris Diderot & CNRS (UMR 7057), 75205 Paris Cedex 13, France.
authors.forename: F
authors.initials: F
authors.lastname: Montel
authors.affiliation: Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon and CNRS, 69364 Lyon Cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Faivre-Moskalenko
authors.affiliation: Laboratoire de Physique, Ecole Normale Supérieure de Lyon, Université de Lyon and CNRS, 69364 Lyon Cedex, France.
authors.forename: M
authors.initials: M
authors.lastname: Castelnovo