title: Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world.
abstract: As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were identified as belonging to Aspergillus, Penicillium and Talaromyces. The aim of this study was to identify isolates to species level and describe the new species found. Secondly, we wanted to create a reliable reference sequence database to be used for next-generation sequencing projects. Isolates represented 59 Aspergillus species, including eight undescribed species, 49 Penicillium species of which seven were undescribed and 18 Talaromyces species including three described here as new. In total, 568 ITS barcodes were generated, and 391 β-tubulin and 507 calmodulin sequences, which serve as alternative identification markers.
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584 CT Utrecht, The Netherlands.
authors.forename: C M
authors.initials: CM
authors.lastname: Visagie
authors.affiliation: Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirooka
authors.affiliation: Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors.forename: J B
authors.initials: JB
authors.lastname: Tanney
authors.affiliation: Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Whitfield
authors.affiliation: Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Mwange
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584 CT Utrecht, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Meijer
authors.affiliation: Department of Botany, University of Hawaii at Manoa, 3190 Maile Way, Honolulu, HI 96822, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Amend
authors.affiliation: Biodiversity (Mycology), Agriculture and Agri-Food Canada, Ottawa, ON K1A0C6, Canada.
authors.forename: K A
authors.initials: KA
authors.lastname: Seifert
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, NL-3584 CT Utrecht, The Netherlands.
authors.forename: R A
authors.initials: RA
authors.lastname: Samson
keywords: Environmental metagenomics; Eurotiales; Indoor moulds; Trichocomaceae