title: Non-invasive delivery of dsGST is lethal to the sweet potato whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae).
abstract: The sweet potato whitefly, Bemisia tabaci (G.) biotype B (Hemiptera: Aleyrodidae), is one of the most economically important pest, by being a dreaded vector of Geminiviruses, and also causes direct damage to the crops by sucking phloem sap. Glutathione S-transferase (GST) is a large family of multifunctional enzymes that play pivotal roles in the detoxification of secondary allelochemical produced by the host plants and in insecticide resistance, thus regulates insect growth and development. The objective of this study is to show the potential of RNA interference (RNAi) in the management of B. tabaci. RNAi is a sequence-specific gene silencing mechanism induced by double-stranded RNA (dsRNA) which holds tremendous potential in pest management. In this regard, we sequenced the GST from B. tabaci and synthesized approximately 500-bp dsRNA from the above and delivered through diet to B. tabaci. Real-time quantitative PCR (RT-qPCR) showed that continuous application of dsGST at 1.0, 0.5, and 0.25 μg/μl reduced mRNA expression levels for BtGST by 77.43, 64.86, and 52.95 % which resulted in mortality by 77, 59, and 40 %, respectively, after 72 h of application. Disruption of BtGST expression will enable the development of novel strategies in pest management and functional analysis of vital genes in B. tabaci.
authors.affiliation: Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake (PO), Bangalore, 560 089, India, asokaniihr@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Asokan
authors.forename: K B
authors.initials: KB
authors.lastname: Rebijith
authors.forename: H K
authors.initials: HK
authors.lastname: Roopa
authors.forename: N K Krishna
authors.initials: NK
authors.lastname: Kumar
mesh_terms: D000284:Administration, Oral; D000818:Animals; D017791:Geminiviridae / Q000502:physiology; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D006430:Hemiptera / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D019476:Insect Proteins / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D007303:Insect Vectors; D027723:Ipomoea batatas / Q000469:parasitology; D010802:Phylogeny; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000138:chemical synthesis / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; D005982:Glutathione Transferase