title: Developing electrochemical sensor for point-of-care diagnostics of oxidative stress marker using imprinted bimetallic Fe/Pd nanoparticle.
abstract: A novel electrochemical-sensing platform based on imprinted bimetallic Fe/Pd (BI-Fe/Pd) nanoparticle has been fabricated for point-of-care diagnostics of oxidative stress marker (3-nitrotyrosine) in biological fluids. Herein, BI-Fe/Pd nanoparticles are used as a platform on which 3-nitrotyrosine imprinted cavities are created using acrylamide as monomer and N-N'-methylene bisacrylamide as cross-linker. The performance of the obtained imprinted sensor is investigated by cyclic, differential pulse, and square wave voltammetry in stripping mode. The imprinted sensor exhibits high recognition ability and affinity for 3-nitrotyrosine in comparison with the non-imprinted one. In addition, the proposed sensor is capable of measuring 3-nitrotyrosine in aqueous as well as in human blood serum, plasma, and urine samples within the range of 4.90-867.57 µg L(-1) and 9.90-867.57 µg L(-1) with detection limit of 1.20 µg L(-1) and 3.25 µg L(-1) by square wave and differential pulse stripping voltammetry, respectively. Imprinted BI-Fe/Pd nanoparticle modified sensor shows high affinity and no interference from blood or urine components. Modified sensor was stored for 45 days at room temperature without any detrimental effects to their binding properties. The high affinity of proposed sensor and the lack of requirement for cold chain logistics make them an attractive alternative to the enzyme-linked immunosorbent assay (ELISA) technique.
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad 826 004, Jharkhand, India.
authors.forename: Ekta
authors.initials: E
authors.lastname: Roy
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad 826 004, Jharkhand, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Patra
authors.affiliation: Department of Applied Chemistry, Indian School of Mines, Dhanbad 826 004, Jharkhand, India. Electronic address: rshmmadhuri@gmail.com.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Madhuri
authors.affiliation: Functional Nanomaterials Research Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad 826 004, Jharkhand, India.
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
mesh_terms: D020106:Acrylamide / Q000737:chemistry; D000178:Acrylamides / Q000737:chemistry; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002138:Calibration; D055664:Electrochemical Techniques; D006801:Humans; D007501:Iron / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress; D010165:Palladium / Q000737:chemistry*; D019095:Point-of-Care Systems; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine
chemical_list: D000178:Acrylamides; D015415:Biomarkers; D020106:Acrylamide; C002744:3-nitrotyrosine; D014443:Tyrosine; D010165:Palladium; D007501:Iron; C021221:N,N'-methylenebisacrylamide
keywords: 3-Nitrotyrosine; Bimetalic Fe/Pd nanoparticle; ELISA; Electrochemical sensor; Surface imprinting