title: miR-466 is putative negative regulator of Coxsackie virus and Adenovirus Receptor.
abstract: This study aimed at elucidating how Coxsackie B virus (CVB) perturbs the host's microRNA (miRNA) regulatory pathways that lead to antiviral events. The results of miRNA profiling in rat pancreatic cells infection models revealed that rat rno-miR-466d was up-regulated in CVB infection. Furthermore, in silico studies showed that Coxsackie virus and Adenovirus Receptor (CAR), a cellular receptor, was one of the rno-miR-466d targets involved in viral entry. Subsequent experiments also proved that both the rno-miR-466d and the human hsa-miR-466, which are orthologs of the miR-467 gene family, could effectively down-regulate the levels of rat and human CAR protein expression, respectively.
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong.
authors.forename: W Y
authors.initials: WY
authors.lastname: Lam
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Ariel C Y
authors.initials: AC
authors.lastname: Cheung
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Christine K C
authors.initials: CK
authors.lastname: Tung
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Apple C M
authors.initials: AC
authors.lastname: Yeung
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Karry L K
authors.initials: KL
authors.lastname: Ngai
authors.affiliation: Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong.
authors.forename: Vivian W Y
authors.initials: VW
authors.lastname: Lui
authors.affiliation: Department of Microbiology, The Chinese University of Hong Kong, Hong Kong; Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong.
authors.forename: Paul K S
authors.initials: PK
authors.lastname: Chan
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong. Electronic address: kwtsui@cuhk.edu.hk.
authors.forename: Stephen K W
authors.initials: SK
authors.lastname: Tsui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein / Q000737:chemistry / Q000235:genetics*; D029822:Enterovirus B, Human / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: C568773:Clmp protein, rat; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; C585904:MIRN466 microRNA, human; C000601998:MIRN466 microRNA, rat; D035683:MicroRNAs
keywords: Coxsackie B virus; Coxsackie virus and Adenovirus Receptor; microRNA