title: Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1.
abstract: Musashi1 is an RNA binding protein that controls the neural cell fate, being involved in maintaining neural progenitors in their proliferative state. In particular, its downregulation is needed for triggering early neural differentiation programs. In this study, we profiled microRNA expression during the transition from neural progenitors to differentiated astrocytes and underscored 2 upregulated microRNAs, miR-23a and miR-125b, that sinergically act to restrain Musashi1 expression, thus creating a regulatory module controlling neural progenitor proliferation.
authors.affiliation: a Deptartment of Biology and Biotechnology Charles Darwin ; Sapienza University of Rome ; Rome, Italy.
authors.forename: Ubaldo
authors.initials: U
authors.lastname: Gioia
authors.forename: Valerio
authors.initials: V
authors.lastname: Di Carlo
authors.forename: Pasquale
authors.initials: P
authors.lastname: Caramanica
authors.forename: Camilla
authors.initials: C
authors.lastname: Toselli
authors.forename: Antonella
authors.initials: A
authors.lastname: Cinquino
authors.forename: Marcella
authors.initials: M
authors.lastname: Marchioni
authors.forename: Pietro
authors.initials: P
authors.lastname: Laneve
authors.forename: Stefano
authors.initials: S
authors.lastname: Biagioni
authors.forename: Irene
authors.initials: I
authors.lastname: Bozzoni
authors.forename: Emanuele
authors.initials: E
authors.lastname: Cacci
authors.forename: Elisa
authors.initials: E
authors.lastname: Caffarelli
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007124:Immunoenzyme Techniques; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015533:Transcriptional Activation
chemical_list: D035683:MicroRNAs; C527468:Mirn125 microRNA, mouse; C534062:Mirn23b microRNA, mouse; C104786:Msi1h protein, mouse; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: 3′UTR, 3′Untranslated Region; BrdU, 5-bromo-2′-deoxyuridine; Msi1, Musashi1; NSPCs, Neural Stem/Progenitor Cells; astrocyte differentiation; cell proliferation; miRNA, microRNA; microRNAs; musashi1; neural progenitors; post-transcriptional gene regulation