title: Two new triterpenoid saponins from rhizome of Anemone amurensis.
abstract: Two new triterpenoid saponins were isolated from the 70% ethanol extract of the rhizome of Anemone amurensis, they are oleanolic acid 28-O-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 4)-β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl ester (1) and 23,27-dihydroxy oleanolic acid 3-O-α-l-arabinopyranoside (2). The structures of 1 and 2 were elucidated on the basis of chemical and spectral analysis, including 1D and 2D NMR data and HR-ESI-MS. Compounds 1 and 2 were tested for cytotoxicities against three human cancer cell lines (A549, Hep-G2, and MCF-7). Compound 1 showed potent cytotoxicity with IC50 values of 34.76, 41.17, and 28.92 μM, respectively, while compound 2 with IC50>100 μM.
authors.affiliation: a School of Traditional Chinese Materia Medica , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Chong-Ning
authors.initials: CN
authors.lastname: Lv
authors.forename: Li
authors.initials: L
authors.lastname: Fan
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Ru-Lan
authors.initials: RL
authors.lastname: Qin
authors.forename: Tan-Ye
authors.initials: TY
authors.lastname: Xu
authors.forename: Tian-Li
authors.initials: TL
authors.lastname: Lei
authors.forename: Jin-Cai
authors.initials: JC
authors.lastname: Lu
mesh_terms: D031883:Anemone / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C000598649:23,27-dihydroxy oleanolic acid 3-O-alpha-L-arabinopyranoside; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D012503:Saponins; C000598648:oleanolic acid 28-O-beta-D-glucopyranosyl-(1-3)-alpha-L-rhamnopyranosyl-(1-4)-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl ester; D009828:Oleanolic Acid
keywords: Anemone amurensis; NMR; cytotoxic activity; triterpenoid saponins