title: The effect of local bending on gating of MscL using a representative volume element and finite element simulation.
abstract: Many physiological processes such as cell division, endocytosis and exocytosis cause severe local curvature of the cell membrane. Local curvature has been shown experimentally to modulate numerous mechanosensitive (MS) ion channels. In order to quantify the effects of local curvature we introduced a coarse grain representative volume element for the bacterial mechanosensitive ion channel of large conductance (MscL) using continuum elasticity. Our model is designed to be consistent with the channel conformation in the closed and open states to capture its major continuum rheological behavior in response to the local membrane curvature. Herein we show that change in the local curvature of the lipid bilayer can modulate MscL activity considerably by changing both bilayer thickness and lateral pressure profile. Intriguingly, although bending in any direction results in almost the same free-energy cost, inward (cytoplasmic) bending favors channel opening, whereas outward (periplasmic) bending facilitates closing of the narrowest part of the MscL pore. This quantitative study using MscL as a model channel may have wide reaching consequences for the effect of local curvature on the physiological function of other types of prokaryotic and eukaryotic membrane proteins.
authors.forename: Omid
authors.initials: O
authors.lastname: Bavi
authors.forename: Manouchehr
authors.initials: M
authors.lastname: Vossoughi
authors.forename: Reza
authors.initials: R
authors.lastname: Naghdabadi
authors.forename: Yousef
authors.initials: Y
authors.lastname: Jamali
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001696:Biomechanical Phenomena; D003198:Computer Simulation; D020342:Finite Element Analysis; D015640:Ion Channel Gating; D008051:Lipid Bilayers / Q000737:chemistry; D008244:Lysophosphatidylcholines / Q000378:metabolism; D008968:Molecular Conformation; D011312:Pressure
chemical_list: D001426:Bacterial Proteins; D008051:Lipid Bilayers; D008244:Lysophosphatidylcholines