title: Anti-proliferative effects of Siegesbeckia orientalis ethanol extract on human endometrial RL-95 cancer cells.
abstract: Endometrial cancer is a common malignancy of the female genital tract. This study demonstrates that Siegesbeckia orientalis ethanol extract (SOE) significantly inhibited the proliferation of RL95-2 human endometrial cancer cells. Treating RL95-2 cells with SOE caused cell arrest in the G2/M phase and induced apoptosis of RL95-2 cells by up-regulating Bad, Bak and Bax protein expression and down-regulation of Bcl-2 and Bcl-xL protein expression. Treatment with SOE increased protein expression of caspase-3, -8 and -9 dose-dependently, indicating that apoptosis was through the intrinsic and extrinsic apoptotic pathways. Moreover, SOE was also effective against A549 (lung cancer), Hep G2 (hepatoma), FaDu (pharynx squamous cancer), MDA-MB-231 (breast cancer), and especially on LNCaP (prostate cancer) cell lines. In total, 10 constituents of SOE were identified by Gas chromatography-mass analysis. Caryophyllene oxide and caryophyllene are largely responsible for most cytotoxic activity of SOE against RL95-2 cells. Overall, this study suggests that SOE is a promising anticancer agent for treating endometrial cancer.
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jingmei0112@hotmail.com.
authors.forename: Chi-Chang
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan. fen153848@yahoo.com.tw.
authors.forename: Hsia-Fen
authors.initials: HF
authors.lastname: Hsu
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jingmei0112@hotmail.com.
authors.forename: Kuo-Hung
authors.initials: KH
authors.lastname: Huang
authors.affiliation: Department of Obstetrics & Gynecology, E-Da Hospital, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan. jingmei0112@hotmail.com.
authors.forename: Jing-Mei
authors.initials: JM
authors.lastname: Wu
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung 82445, Taiwan. smkuo@isu.edu.tw.
authors.forename: Shyh-Ming
authors.initials: SM
authors.lastname: Kuo
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. fen153848@yahoo.com.tw.
authors.forename: Xue-Hua
authors.initials: XH
authors.lastname: Ling
authors.affiliation: Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan. jyhoung@isu.edu.tw.
authors.forename: Jer-Yiing
authors.initials: JY
authors.lastname: Houng
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae / Q000737:chemistry*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000473:pathology*; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D000431:Ethanol