title: Molecular cloning of two structure variants of crustacean hyperglycemic hormone (CHH) from the swimming crab (Portunus trituberculatus), and their gene expression during molting and ovarian development.
abstract: Two full-length cDNA (Pt-CHH1 and Pt-CHH2) sequences encoding crustacean hyperglycemic hormone (CHH) were cloned from tissues of the swimming crab (Portunus trituberculatus) using RACE. Pt-CHH1 was cloned from eyestalk, whereas Pt-CHH2 was cloned from thoracic ganglia. Sequence and structure analyses of Pt-CHH1 and Pt-CHH2 suggest that they may be generated from alternative splicing. Tissue distribution showed that transcript of Pt-CHH1 was only detected in eyestalk, while transcript of Pt-CHH2 was observed in several extra-eyestalk tissues. The transcript levels of Pt-CHH1 and Pt-CHH2 during molting and ovarian development were determined using qPCR. In molting process, level of Pt-CHH1 in eyestalk increased from stage A (postmolt), and to significant higher at stage C (intermolt), then decreased during premolt (D0-D4). In ovarian development, level of Pt-CHH1 in eyestalk decreased from previtellogenic stage (II), and to significant lower at mature stage (IV). The expression patterns of Pt-CHH2 in thoracic ganglia and Y-organ were distinct from that of Pt-CHH1 in eyestalk. The combined results suggest that Pt-CHH1 may be involved in inhibition of molting and ovarian development, whereas Pt-CHH2 may have other physiological functions.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo 315211, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xie
authors.forename: Dongfa
authors.initials: D
authors.lastname: Zhu
authors.forename: Jifen
authors.initials: J
authors.lastname: Yang
authors.forename: Xier
authors.initials: X
authors.lastname: Qiu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Cui
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003386:Brachyura / Q000378:metabolism*; D003001:Cloning, Molecular; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D007447:Invertebrate Hormones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018978:Molting / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010053:Ovary / Q000502:physiology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D060829:Arthropod Proteins; D007447:Invertebrate Hormones; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C015947:hyperglycemic hormone, crustacean
keywords: Portunus trituberculatus; cloning; crustacean hyperglycemic hormone; gene expression; molting; ovarian development