title: The effect of thalidomide on ethanol-induced gastric mucosal damage in mice: involvement of inflammatory cytokines and nitric oxide.
abstract: Excessive ethanol ingestion causes gastric mucosal damage through the inflammatory and oxidative processes. The present study was aimed to evaluate the protective effect of thalidomide on ethanol-induced gastric mucosal damage in mice. The animals were pretreated with vehicle or thalidomide (30 or 60 mg/kg, orally), and one hour later, the gastric mucosal injury was induced by oral administration of acidified ethanol. The animals were euthanized one hour after ethanol ingestion, and gastric tissues were collected to biochemical analyzes. The gastric mucosal lesions were assessed by macroscopic and histopathological examinations. The results showed that treatment of mice with thalidomide prior to the administration of ethanol dose-dependently reduced the gastric ulcer index. Thalidomide pretreatment significantly reduced the levels of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6], malondialdehyde (MDA) and myeloperoxidase (MPO) activity. In addition, thalidomide significantly inhibited ethanol-induced nitric oxide (NO) overproduction in gastric tissue. Histological observations showed that ethanol-induced gastric mucosal damage was attenuated by thalidomide pretreatment. It seems that thalidomide as an anti-inflammatory agent may have a protective effect against alcohol-induced mucosal damage by inhibition of neutrophil infiltration and reducing the production of nitric oxide and inflammatory cytokines in gastric tissue.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran. Electronic address: k.amirshahrokhi@arums.ac.ir.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amirshahrokhi
authors.affiliation: Division of Pathology, Imam Hospital, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Ali-Reza
authors.initials: AR
authors.lastname: Khalili
mesh_terms: D000818:Animals; D000431:Ethanol / Q000378:metabolism* / Q000633:toxicity; D005260:Female; D006651:Histocytochemistry; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000032:analysis / Q000276:immunology; D015850:Interleukin-6 / Q000032:analysis / Q000276:immunology; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000276:immunology; D009195:Peroxidase / Q000032:analysis / Q000276:immunology; D011897:Random Allocation; D013272:Stomach Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology; D013276:Stomach Ulcer / Q000139:chemically induced* / Q000188:drug therapy / Q000276:immunology; D013792:Thalidomide / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000276:immunology
chemical_list: D007166:Immunosuppressive Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000431:Ethanol; D008315:Malondialdehyde; D013792:Thalidomide; D009195:Peroxidase
keywords: Ethanol; Gastric mucosal damage; Inflammation; Nitric oxide; Thalidomide