title: Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing.
abstract: The migration of cells from the side and the bottom of the defect is important in osteochondral defect healing. Here, we designed a novel collagen scaffold that possessed channels in both the horizontal and the vertical directions, along with stromal cell-derived factor-1 (SDF-1) to enhance osteochondral regeneration by facilitating cell homing. Firstly we fabricated the radially oriented and random collagen scaffolds, then tested their properties. The radially oriented collagen scaffold had better mechanical properties than the random scaffold, but both supported cell proliferation well. Then we measured the migration of BMSCs in the scaffolds in vitro. The radially oriented collagen scaffold effectively promoted their migration, and this effect was further facilitated by addition of SDF-1. Moreover, we created osteochondral defects in rabbits, and implanted them with random or oriented collagen scaffolds with or without SDF-1, and evaluated cartilage and subchondral bone regeneration at 6 and 12 weeks after surgery. Cartilage regeneration with both the radially oriented scaffold and SDF-1 effectively promoted repair of the cartilage defect. Our results confirmed that the implantation of the radially oriented channel collagen scaffold with SDF-1 could be a promising strategy for osteochondral repair.
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China; Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 3 East Qingchun Road, Hangzhou 310016, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Chen
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Tao
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Shouan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Youzhi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Qijiang
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yu
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang Provincial Key Lab for Tissue Engineering and Regenerative Medicine, Zhejiang University, 866 Yu Hang Tang Road, Hangzhou 310058, China. Electronic address: hwoy@zju.edu.cn.
authors.forename: HongWei
authors.initials: H
authors.lastname: Ouyang
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000737:chemistry* / Q000494:pharmacology; D020219:Chondrogenesis / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D007150:Immunohistochemistry; D008297:Male; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D054377:Chemokine CXCL12; D003094:Collagen
keywords: Cartilage defect repair; Collagen scaffolds; Osteochondral tissue engineering; SDF-1