title: Effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens.
abstract: 1. Bacteriophages (BP) have gained increasing attention as a treatment of bacterial infection for animals. However, the data pertaining to dietary application of BP for laying hens have been limited. 2. This study aimed to investigate the effect of dietary BP on laying performance, egg quality and caecal bacterial populations in laying hens. 3. The dietary BP used in this experiment was a mixture of individual BP targeting Salmonella gallinarum, Salmonella pullorum, Salmonella typhimurium, Salmonella enteritidis, Salmonella derby and Staphylococcus aureus. 4. A total of 360 Hy-Line Brown laying hens of 32 weeks of age were allotted to one of three dietary treatments with 6 replicates in a completely randomised design. The basal diet was prepared, and 0.4 or 0.8 g/kg BP mixture was supplemented to the basal diet. Diets were fed to hens for 8 weeks. 5. Laying performance and egg quality were not affected by dietary treatments. As inclusion levels of BP mixture in diets were increased, the DNA copy numbers for Salmonella spp. in the caecal contents decreased linearly, whereas the DNA copy numbers for Escherichia coli in the caecal contents increased linearly. 6. Results indicate that dietary supplementation of BP mixture decreases the target Salmonella spp. populations but increases Escherichia coli populations in the gastrointestinal tract of laying hens with little impact on laying performance and egg quality.
authors.affiliation: a Department of Animal Science and Technology , Chung-Ang University , Anseong-si , Gyeonggi-do , Republic of Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Kim
authors.forename: H S
authors.initials: HS
authors.lastname: Shin
authors.forename: M C
authors.initials: MC
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.forename: G-B
authors.initials: GB
authors.lastname: Kim
authors.forename: D Y
authors.initials: DY
authors.lastname: Kil
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000502:physiology*; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D005260:Female; D010063:Ovum / Q000187:drug effects / Q000502:physiology; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D012098:Reproduction / Q000187:drug effects; D012475:Salmonella / Q000187:drug effects / Q000821:virology; D012481:Salmonella Infections, Animal / Q000382:microbiology / Q000517:prevention & control*; D012477:Salmonella enteritidis / Q000187:drug effects / Q000821:virology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000821:virology; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000821:virology