title: The acclimation response to high light is initiated within seconds as indicated by upregulation of AP2/ERF transcription factor network in Arabidopsis thaliana.
abstract: High light acclimation implicates mechanisms on various molecular levels and time scales. The recently identified small transcription factor network of APETALA 2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factors is triggered upon transfer of Arabidopsis to high light and depends on metabolite export and mitogen activated protein kinase activation. An experimental design was developed consisting of a low light to high light and back to low light illumination. This allowed the determination of the time point of no return post high light transfer which activates transcription of the AP2/ERF network. Within 10 seconds of high light treatment transcript levels of ERF6, ERF104, ERF105 and RRTF were triggered to increase from low to high levels within the next 10 minutes witnessing an ultrafast retrograde pathway with a very early time point of no return. This response differed profoundly from other high light-responsive transcripts such as stromal ascorbate peroxidase (sAPX) which accumulated in a dose-dependent manner or COR47.
authors.affiliation: a Biochemistry and Physiology of Plants ; Bielefeld University ; Bielefeld , Germany.
authors.forename: M
authors.initials: M
authors.lastname: Moore
authors.forename: Mo
authors.initials: M
authors.lastname: Vogel
authors.forename: Kj
authors.initials: K
authors.lastname: Dietz
mesh_terms: D000064:Acclimatization / Q000235:genetics / Q000528:radiation effects*; D017360:Arabidopsis / Q000235:genetics* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D053263:Gene Regulatory Networks / Q000528:radiation effects*; D018398:Homeodomain Proteins / Q000378:metabolism*; D008027:Light; D055537:Light Signal Transduction / Q000528:radiation effects; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000528:radiation effects*
chemical_list: C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: A. thaliana, Arabidopsis thaliana; ABA, Abscisic Acid; AP2/ERF, APETALA2/ETHYLENE RESPONSE FACTOR; ETC, electron transport chain; H-light, High Light (800 μmol quanta m−2 s−1); L-light, Low Light (8 μmol quanta m−2 s−1); LH→L, Low Light to High Light to Low Light transfer; Low Light to High Light transfer; ROS, reactive oxygen species; SA, Salicylic Acid; TF, Transcription Factor; L→H; WWC, water-water cycle; acclimation; chloroplast; light; log2, logarithmic fold change to base 2; photosynthesis; transcription factor