title: Applicability of human dental follicle cells to bone regeneration without dexamethasone: an in vivo pilot study.
abstract: The aim of this study was to investigate the capacity of human dental follicle cells (hDFCs) for bone formation in vivo. hDFCs were obtained from wisdom teeth extracted from patients aged 14 and 22 years. hDFCs from the 5th to 8th passages were grown in three-dimensional (3D) culture using gelatin sponges. Cells were transplanted onto the calvaria of F344/NJcl-rnu/rnu male rats (immunodeficient rats). Haematoxylin and eosin (HE) staining and immunohistochemistry were performed, and newly formed bone was evaluated by micro-computed tomography (micro-CT). HE staining showed newly formed bone in 3D culture. Immunohistochemistry showed bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), and osterix staining in areas with newly formed bone. Furthermore, micro-CT showed that, in comparison to controls, transplanted hDFCs promoted better bone quality and bone mineral density (BMD 582 ± 131.1 vs. 300.5 ± 77.7 mg/cm(3); P=0.039), bone mineral content (BMC 5.6 ± 1.1 vs. 2.1 ± 0.4 mg; P = 0.006), bone volume (BV 9.7 ± 0.5 × 10(-3) vs. 7.0 ± 0.4 × 10(-3) cm(3); P = 0.002), BMC/total volume (TV) (399.9 ± 76.3 vs. 147.7 ± 30.8 mg/cm(3); P = 0.006), and BV/TV (69.1 ± 3.6% vs. 49.6 ± 3.1%; P=0.002). This suggests that human dental follicles are potentially useful for regenerative therapy.
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan. Electronic address: takahashi.kosuke@nihon-u.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Ogura
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Tomoki
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Eda
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okada
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Kato
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iwai
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuyama
authors.affiliation: Department of Maxillofacial Surgery, Department of Oral Pathology, Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kondoh
mesh_terms: D000293:Adolescent; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D003795:Dental Sac / Q000166:cytology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D010865:Pilot Projects; D051381:Rats; D011916:Rats, Inbred F344; D055114:X-Ray Microtomography; D055815:Young Adult
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human
keywords: bone regeneration; dental follicle; osteogenic differentiation