title: N-acetyl lysine/glutathione-derived pyrroles as potential ex vivo biomarkers of bioactivated furan-containing compounds.
abstract: Many furan-containing compounds have been reported to be cytotoxic and/or carcinogenic agents. The toxic furans exert their adverse effects possibly through metabolic activation of the furans to corresponding epoxides or/and cis-enediones. Detection of the reactive metabolites is a challenge since the electrophiles often have short lives in vivo, and they are too reactive to be isolated for characterization. Seven compounds, including 2,5-dimethylfuran, R-(+)-menthofuran, R-(+)-pulegone, caesalmin C, furanodiene, diosbulbin B, and limonin, were selected for a biomarker search study. Glutathione (GSH) conjugates derived from the test compounds were detected in bile of rats, and the types of the biliary GSH conjugates observed differed from each other and were unpredictable. However, upon mixing of the bile with a solution of N-acetyl lysine (NAL), pyrroles derived from NAL and GSH were exclusively detected in all the bile samples without any exception. The formation of the pyrrole-NAL/GSH conjugates was verified by microsomal incubations and chemical synthesis. The findings facilitate the development of in vivo biomarkers of metabolic activation of furanoids.
authors.affiliation: School of Traditional Chinese Medicine, ‡School of Pharmacy, §Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University , Shenyang, Liaoning 110016, P. R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.forename: Dongju
authors.initials: D
authors.lastname: Lin
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Gao
authors.forename: Huiming
authors.initials: H
authors.lastname: Hua
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Jiang
authors.initials: J
authors.lastname: Zheng
mesh_terms: D065767:Activation, Metabolic; D000818:Animals; D001646:Bile / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D005663:Furans / Q000493:pharmacokinetics*; D005978:Glutathione / Q000378:metabolism*; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism*; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D005663:Furans; D011758:Pyrroles; D005978:Glutathione; D008239:Lysine