title: Gamma radiation induced cell cycle perturbations and DNA damage in Catla Catla as measured by flow cytometry.
abstract: Gamma radiation induced cell cycle perturbations and DNA damage in Catla catla were analyzed in erythrocytes at different time points using flow cytometry (FCM). Protracted exposure to radiation induced damage between days 12 and 45. Disturbances in cell cycle machinery, i.e., proportional increase and decrease in Gap0 or quiescent/Gap1 (G0/G1), Synthesis (S) and Gap2/Mitotic (G2/M) phases were observed at both acute and protracted treatments. Both acute and protracted exposures induced apoptosis with a notable significance between days 3 and 6 at protracted and on day 45 at acute doses. Fish exposed protractedly avail some DNA repair mechanisms than acutely exposed. This is the first study to analyze radiation induced DNA damage under laboratory conditions and suggests that flow cytometry can also be an alternate tool to screen genotoxicity induced by ionizing radiation in fish.
authors.affiliation: Radiological Safety Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamilnadu 603 102, India. Electronic address: aquatox1982@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Anbumani
authors.affiliation: Radiological Safety Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamilnadu 603 102, India. Electronic address: marynmk@gmail.com.
authors.forename: Mary N
authors.initials: MN
authors.lastname: Mohankumar
mesh_terms: D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle / Q000528:radiation effects*; D003530:Cyprinidae / Q000235:genetics; D004249:DNA Damage; D004912:Erythrocytes / Q000528:radiation effects; D005434:Flow Cytometry; D005720:Gamma Rays
keywords: Cell cycle; DNA damage; Fish; Flow cytometry; Gamma radiation