title: Upregulation of stromal cell-derived factor 1 (SDF-1) is associated with macrophage infiltration in renal ischemia-reperfusion injury.
abstract: BACKGROUND
  Stromal cell-derived factor-1(SDF-1) is a chemotactic and angiogenic factor that mediates the repair of various tissues. As macrophages are important contributors to ischemic kidney injury, we examine the role of SDF-1 in a rodent model of ischemia-reperfusion (I/R) injury.
  
  
  METHODS
  Male wild-type (WT) (C57BL/6) mice were subjected to bilateral I/R injury or sham operation in the presence or absence of macrophage depletion (liposomal clodronate [0.2 ml/20-25 g body weight i.p.]). Macrophage accumulation was assessed by immunohistochemistry. Tissue levels of SDF-1 (ELISA) and SDF-1 mRNA expression (real-time PCR) were measured. The cellular location of SDF-1 was assessed using immunohistochemical staining.
  
  
  RESULTS
  Immunofluorescence staining of renal tissue sections confirmed macrophage depletion by liposomal clodronate. SDF-1 production was elevated in response to I/R injury and was significantly increased upon macrophage depletion. SDF-1 positive cells initially appeared initially in the cortex, and subsequently diffused to the outer medulla after I/R injury.
  
  
  CONCLUSIONS
  Our study demonstrates that SDF-1 is significantly upregulated during renal I/R. We hypothesize that SDF-1 upregulation may be an important macrophage effector mechanism during I/R injury.
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wan
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Wenkai
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Gendoo
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, Department of surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Nephrology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: Changchun
authors.initials: C
authors.lastname: Cao
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D054377:Chemokine CXCL12 / Q000276:immunology*; D004195:Disease Models, Animal; D007668:Kidney / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D015427:Reperfusion Injury / Q000276:immunology* / Q000473:pathology; D015854:Up-Regulation / Q000276:immunology*
chemical_list: D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse