title: Genome annotation provides insight into carbon monoxide and hydrogen metabolism in Rubrivivax gelatinosus.
abstract: We report here the sequencing and analysis of the genome of the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS. This microbe is a model for studies of its carboxydotrophic life style under anaerobic condition, based on its ability to utilize carbon monoxide (CO) as the sole carbon substrate and water as the electron acceptor, yielding CO2 and H2 as the end products. The CO-oxidation reaction is known to be catalyzed by two enzyme complexes, the CO dehydrogenase and hydrogenase. As expected, analysis of the genome of Rx. gelatinosus CBS reveals the presence of genes encoding both enzyme complexes. The CO-oxidation reaction is CO-inducible, which is consistent with the presence of two putative CO-sensing transcription factors in its genome. Genome analysis also reveals the presence of two additional hydrogenases, an uptake hydrogenase that liberates the electrons in H2 in support of cell growth, and a regulatory hydrogenase that senses H2 and relays the signal to a two-component system that ultimately controls synthesis of the uptake hydrogenase. The genome also contains two sets of hydrogenase maturation genes which are known to assemble the catalytic metallocluster of the hydrogenase NiFe active site. Collectively, the genome sequence and analysis information reveals the blueprint of an intricate network of signal transduction pathways and its underlying regulation that enables Rx. gelatinosus CBS to thrive on CO or H2 in support of cell growth.
authors.affiliation: Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming, United States of America.
authors.forename: Karen
authors.initials: K
authors.lastname: Wawrousek
authors.affiliation: Biosciences Center, National Renewable Energy Laboratory, Golden, Colorado, United States of America.
authors.forename: Scott
authors.initials: S
authors.lastname: Noble
authors.affiliation: Pacific Biosciences, Menlo Park, California, United States of America.
authors.forename: Jonas
authors.initials: J
authors.lastname: Korlach
authors.affiliation: Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, United States of America.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming, United States of America.
authors.forename: Carrie
authors.initials: C
authors.lastname: Eckert
authors.affiliation: Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming, United States of America.
authors.forename: Jianping
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, Wyoming, United States of America.
authors.forename: Pin-Ching
authors.initials: PC
authors.lastname: Maness
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D042521:Burkholderiaceae / Q000235:genetics / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism*; D016680:Genome, Bacterial / Q000502:physiology*; D006859:Hydrogen / Q000378:metabolism*; D058977:Molecular Sequence Annotation
chemical_list: D001426:Bacterial Proteins; D002248:Carbon Monoxide; D006859:Hydrogen