title: HIF1A regulates xenophagic degradation of adherent and invasive Escherichia coli (AIEC).
abstract: The hypoxia inducible transcription factor HIF1 activates autophagy, a general catabolic pathway involved in the maintenance of cellular homeostasis. Dysfunction in both autophagy and HIF1 has been implicated in an increasing number of human diseases, including inflammatory bowel disease (IBD), such as Crohn disease (CD). Adherent invasive E. coli (AIEC) colonize ileal mucosa of CD patients and strongly promote gastrointestinal inflammatory disorders by activation of HIF-dependent responses. Here, we aim to characterize the contribution of HIF1 in xenophagy, a specialized form of autophagy involved in the degradation of intracellular bacteria. Our results showed that endogenous HIF1A knockdown increased AIEC survival in intestinal epithelial cells. We demonstrate that the increase in survival rate correlates with a dramatic impairment of the autophagic flux at the autolysosomal maturation step. Furthermore, we show that AIEC remained within single-membrane LC3-II-positive vesicles and that they were unable to induce the phosphorylation of ULK1. These results suggested that, in the absence of HIF1A, AIEC were found within LC3-associated phagosomes. Using blocking antibodies against TLR5 and CEACAM6, the 2 well-known AIEC-bound receptors, we showed that downstream receptor signaling was necessary to mediate ULK1 phosphorylation. Finally, we provide evidence that HIF1 mediates CEACAM6 expression and that CEACAM6 is necessary to recruit ULK1 in a bacteria-containing signaling hub. Collectively, these results identify a new function for HIF1 in AIEC-dedicated xenophagy, and suggest that coactivation of autophagy and HIF1A expression may be a potential new therapy to resolve AIEC infection in CD patients.
authors.affiliation: a Institute for Research on Cancer and Aging; IRCAN U1081 UMR CNRS/UNS 7284 ; Nice , France.
authors.forename: Sanda
authors.initials: S
authors.lastname: Mimouna
authors.forename: Marie
authors.initials: M
authors.lastname: Bazin
authors.forename: Baharia
authors.initials: B
authors.lastname: Mograbi
authors.forename: Arlette
authors.initials: A
authors.lastname: Darfeuille-Michaud
authors.forename: Patrick
authors.initials: P
authors.lastname: Brest
authors.forename: Paul
authors.initials: P
authors.lastname: Hofman
authors.forename: Valérie
authors.initials: V
authors.lastname: Vouret-Craviari
mesh_terms: D001343:Autophagy / Q000502:physiology*; D002460:Cell Line; D003424:Crohn Disease / Q000276:immunology / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000382:microbiology*; D004927:Escherichia coli Infections / Q000378:metabolism*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit
keywords: AIEC, adherent invasive E. coli; AMPK, AMP-activated protein kinase; ATG16L1, autophagy-related 16-like 1; ATG5, autophagy-related 5; BECN1, Beclin 1, autophagy-related; BNIP3L, BCL2/adenovirus E1B 19kDa interacting protein 3-like; CD, Crohn disease; CEACAM6, carcinoembryonic antigen-related cell adhesion molecule 6 (nonspecific cross reacting antigen); CRTC1/TORC1, CREB regulated transcription coactivator 1; Crohn disease; EEA1, early endosome antigen 1; GFP, green fluorescent protein; HBSS, Hank's balanced salt solution; HIF1A, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor); IBD, inflammatory bowel disease; IRGM, immunity-related GTPase family, M; LAP; LAP, LC3-associated phagocytosis; MAP1LC3-II (LC3-II), microtubule-associated protein 1 light chain 3-II; MOI, multiplicity of infection; SQSTM1/p62 (SQSTM1), sequestosome 1; TEM, transmission electron microscopy; TLR5, toll-like receptor 5; ULK1, unc-51 like autophagy activating kinase 1; VAV2, vav 2 guanine nucleotide exchange factor; autophagy; bacteria; hypoxia inducible factor