title: Human G3P[9] rotavirus strains possessing an identical genotype constellation to AU-1 isolated at high prevalence in Brazil, 1997-1999.
abstract: Rotavirus (RV) A is a very common cause of acute diarrhoea in infants and young children worldwide. Most human strains are classified into two major Wa-like and DS-1-like genotype constellations, whilst a minor third strain, AU-1, was described in 1989 among human RV isolates from Japan. AU-1 demonstrates a high degree of homology to a feline RV, FRV-1, which suggests interspecies transmission of feline RV. However, there has been no subsequent report of RVs possessing the AU-1 genotype throughout all 11 genes of the genome. Between March 1997 and December 1999, 157 RV-positive stool samples were collected from Brazilian children, and 16 of the RVs (10.2 %) were P[9] genotype. We analysed eight strains by almost full-genome sequencing. These eight strains were divided into two groups: five AU-1-like and three Wa-like strains. Four of the five AU-1-like strains had the AU-1-like genotype constellation throughout the 11 genes. The remaining AU-1-like strain was considered to be a reassortant strain comprosed of nine, two and one genes from the AU-1-like, Wa-like and G9 strains, respectively. The three Wa-like strains were considered to be reassortants comprising seven to eight genes and three to four genes from Wa-like and non-Wa-like strains, respectively. This report of human G3P[9] RV strains possessing the AU-1 genotype constellation throughout all genes demonstrates the stability and infectivity of the AU-1-like strain with its original genotype over distance and time.
authors.affiliation: Rotavirus Vaccine Development Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, 20892, USA.|Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tsugawa
authors.affiliation: Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA.|Rotavirus Vaccine Development Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, 20892, USA.
authors.forename: Kaitlin
authors.initials: K
authors.lastname: Rainwater-Lovett
authors.affiliation: Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, 060-8543, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsutsumi
mesh_terms: D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D017124:Conserved Sequence; D003967:Diarrhea / Q000821:virology; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D016865:Reassortant Viruses; D012401:Rotavirus / Q000145:classification* / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*
chemical_list: D012367:RNA, Viral