title: Angiogenic and innate immune responses triggered by PRP in tendon cells are not modified by hyperuricemia.
abstract: BACKGROUND
  hyperuricemia is becoming a critical medical problem, and a current focus of research. Uric acid is also a death cell associated stressor that may trigger innate immune responses via the synthesis of inflammatory and angiogenic proteins.
  
  
  OBJECTIVE
  to investigate the angiogenic/inflammatory protein profile of tendon cells treated with Platelet Rich Plasma (PRP), and to assess if there are any differences in synthesis of angiogenic/inflammatory cytokines between PRP-treated or hyperuricemic PRP-treated cells.
  
  
  METHODS
  tendon cells were treated with PRP or hyperuricemic PRP and cell culture supernatants examined using glass based protein arrays for inflammation and angiogenesis. Relevant proteins were subsequently quantified by ELISA or EASIA methods.
  
  
  RESULTS
  the impact of PRP on angiogenesis and inflammation is evidenced by relevant cytokine synthesis including: Monocyte Chemoattractant Protein (MCP-1/CCL2), Regulated upon Activation Normally T cells Expressed and Secreted (RANTES/CCL5), IL-6/CXCL6, IL-8/CXCL8, Vascular Endothelial Growth Factor (VEGF), Growth Regulated Protein (GRO-a/CXCL1) and Hepatocyte Growth Factor (HGF). IL-1beta was not detected in these conditions. Taken together these data suggest an initial angiogenetic and innate immune responses driven by chemokines that is not altered by the presence of hyperuricemia, at this point, except for IL-8 secretion, p= 0.042.
authors.affiliation: Regenerative Medicine Laboratory, BioCruces, Health Research Institute, Cruces University Hospital Barakaldo, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Andia
authors.affiliation: Regenerative Medicine Laboratory, BioCruces, Health Research Institute, Cruces University Hospital Barakaldo, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Rubio-Azpeitia
keywords: PRP; chemokines; growth factors; tenocyte; uric acid