title: Upregulated circulating miR-150 is associated with the risk of intrahepatic cholangiocarcinoma.
abstract: During the last decade, microRNAs (miRNAs) have been identified as potential biomarkers and therapeutic targets for multiple malignancies; yet, few studies exist on intrahepatic cholangiocarcinoma (ICC). In the present study, a miRNA microarray was applied to determine the significant miRNAs involved in ICC. miR-150 was found to be significantly downregulated in ICC. We further enrolled 15 ICC patients who received radical resection to test these findings in plasma. Using quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we examined and quantified the expression levels of miR-150 in tumor tissues, peritumoral noncancerous tissues and blood samples of 15 ICC patients. The diagnostic value of plasma miR-150 for differentiating patients with ICC from the age- and gender-matched controls was analyzed. For plasma samples, compared with normal controls, the level of miR-150 expression was found to be upregulated (P<0.010) in ICC patients. While differentiating ICC from normal controls, receiver operator curve (ROC) analysis of plasma miR-150 revealed the area under the curve (AUC) of 0.764 (P<0.010) with sensitivity of 80.6% and specificity of 58.1%. The diagnostic value of carbohydrate antigen 19-9 (CA19-9) and the combination of miR-150 and CA19-9 were also evaluated. We found that the combination of these two markers improved the power of screening ICC. Moreover, on the basis of the plasma miR-150 level, 15 ICC patients were divided into a low or high expression group. We found that plasma miR-150 is a potential diagnostic biomarker for ICC.
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Shouli
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Jikai
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of General Surgery, General Hospital of Beijing Military Command, Beijing 100700, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: He
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Xilin
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of General Surgery, Tangdu Hospital of The Fourth Military Medical University, Shaanxi 710038, P.R. China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Lu
mesh_terms: D000368:Aged; D001650:Bile Duct Neoplasms / Q000097:blood* / Q000235:genetics / Q000601:surgery; D001653:Bile Ducts, Intrahepatic / Q000601:surgery; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics*; D018395:CA-19-9 Antigen / Q000378:metabolism; D018281:Cholangiocarcinoma / Q000097:blood* / Q000235:genetics / Q000601:surgery; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012372:ROC Curve; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D035683:MicroRNAs; C533097:MIR150, human