title: Thermodynamic investigation of interaction between [(η6-p-cymene) RuII(acetone-N4-phenylthiosemicarbazone)Cl]Cl anticancer drug and human serum albumin: spectroscopic and electrochemical studies.
abstract: In this contribution, the interaction between [(η (6)-p-cymene)Ru(II)(acetone-N (4)-phenylthiosemicarbazone)Cl]Cl (Ru-TSC) anticancer drug and human serum albumin (HSA) was investigated by spectroscopic and electrochemical techniques. The fluorescence spectra results indicated that Ru-TSC anticancer drug could quench the intrinsic fluorescence of HSA through dynamic quenching mode. The calculated corresponding activation energy of the interaction between Ru-TSC anticancer drug and HSA was 35.62 kJ mol(-1). The distance between HSA and Ru-TSC anticancer drug was obtained according to fluorescence resonance energy transfer. The results of synchronous fluorescence spectra, three-dimensional fluorescence spectra, Fourier transform infrared spectroscopy (FTIR) spectra, and circular dichroism (CD) spectra indicated that the microenvironment and the conformation of HSA were all changed in the presence of Ru-TSC anticancer drug. The results of cyclic voltammetry further validated the interaction between Ru-TSC and HSA. These results indicated that the biological activity of HSA was affected by Ru-TSC anticancer drug dramatically.
authors.affiliation: College of Chemistry and Material Science, Guangxi Teachers Education University, Nanning, 530001, People's Republic of China.
authors.forename: Shan
authors.initials: S
authors.lastname: Huang
authors.forename: Fawei
authors.initials: F
authors.lastname: Zhu
authors.forename: Quanquan
authors.initials: Q
authors.lastname: Qian
authors.forename: Qi
authors.initials: Q
authors.lastname: Xiao
authors.forename: Wei
authors.initials: W
authors.lastname: Su
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D002942:Circular Dichroism; D004563:Electrochemistry; D006801:Humans; D017975:Ruthenium Compounds / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics
chemical_list: D000970:Antineoplastic Agents; D017975:Ruthenium Compounds; D012709:Serum Albumin