title: Selective glycosylation of steroidal saponins by Arthrobacter nitroguajacolicus.
abstract: In this study seven strains of the genus Arthrobacter were screened by biotransformation to discover glycosylating patterns on steroid saponins. A strain of Arthrobacter nitroguajacolicus (CPCC 203516) was found to have the ability of fructosylation. Crude enzyme of the strain was extracted for the further study of conversion characteristics and patterns. Sucrose was used as a non-activated sugar donor, and fifteen steroidal saponins were involved. Nine furostan saponins of the substrates were converted, and ten products were isolated and identified. Based on the HR-ESI-MS, 1D, and 2D NMR spectral data, one fructosyl was added to furostan saponins at C6-OH of 26-O-β-D-glucopyranosyl by A. nitroguajacolicus for all nine products. One product was distinguished by an additional fructosyl at the position of C6-OH on the first added fructosyl. Spirostan saponins of the substrates could not be converted. Steroidal saponins embracing a fructosyl are quite rare according to other reports based on similar studies. This study successfully converted furostan saponins into new compounds.
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China; Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. Electronic address: liujingyuan08@foxmail.com.
authors.forename: Jing-yuan
authors.initials: JY
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: comic-3344@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: kang_liping21@163.com.
authors.forename: Li-ping
authors.initials: LP
authors.lastname: Kang
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: liu_yix@163.com.
authors.forename: Yi-xun
authors.initials: YX
authors.lastname: Liu
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: mmyzhao@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: xiongchengqi2005@163.com.
authors.forename: Cheng-qi
authors.initials: CQ
authors.lastname: Xiong
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: zhyuqin@126.com.
authors.forename: Yu-qin
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address: yly@cpcc.ac.cn.
authors.forename: Li-yan
authors.initials: LY
authors.lastname: Yu
authors.affiliation: Beijing Institute of Radiation Medicine, No. 27 Tai-ping Road, Haidian District, Beijing 100850, China. Electronic address: mabaiping@sina.com.
authors.forename: Bai-ping
authors.initials: BP
authors.lastname: Ma
mesh_terms: D001173:Arthrobacter / Q000378:metabolism*; D001711:Biotransformation; D005632:Fructose / Q000378:metabolism; D006031:Glycosylation; D012503:Saponins / Q000737:chemistry* / Q000378:metabolism*; D013256:Steroids / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D012503:Saponins; D013256:Steroids; D005632:Fructose
keywords: Arthrobacter nitroguajacolicus; Biotransformation; Fructosylation; Steroidal saponin