title: A novel folic acid-conjugated TiO₂-SiO₂ photosensitizer for cancer targeting in photodynamic therapy.
abstract: In this paper, a novel folic acid-conjugated silica-coated titanium dioxide (TiO2-SiO2) photosensitizer was synthesized and characterized using various analytical instruments. The photosensitizer was further assessed with regards to its photoreactivity, cellular and hemocompatibility, cell internalization, and phototoxicity. Conjugating folic acid with TiO2-SiO2 has shown a significantly improved compatibility of the nanoparticles with the mouse fibroblast cells (L929) at 24 h. An improved compatibility with the human nasopharyngeal epidermoid cancer (KB) cells was also demonstrated, but to a slightly reduced degree. Enhanced cell internalization was well demonstrated in the TiO2-SiO2 folate nanoparticles. Upon exposure to UV light, TiO2-SiO2 folate nanoparticles maintained a high level photodynamic reactivity and yielded a 38-43% photo-killing of KB cells. The photo-killing effect increased with increasing dosage in the investigated concentration range of 50-100 μg ml(-1).
authors.affiliation: Department of Chemical Engineering, Curtin University, Kent Street, Bentley, WA 6102, Australia.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Orthopedics, The First Hospital of Jilin University, China.
authors.forename: Shaokun
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The First Hospital of Jilin University, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Chemical Engineering, Curtin University, Kent Street, Bentley, WA 6102, Australia. Electronic address: X.Lou@curtin.edu.au.
authors.forename: Xia
authors.initials: X
authors.lastname: Lou
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects; D005492:Folic Acid / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D017319:Photosensitizing Agents; C507641:TiO2-SiO2; D012822:Silicon Dioxide; D005492:Folic Acid; D014025:Titanium
keywords: Folic acid; Photodynamic therapy; Photosensitizer, Photo-killing; Targeting; Titanium dioxide nanoparticles