title: Zebrafish embryo intersegmental vessels: a tool for investigating sprouting angiogenesis.
abstract: Angiogenesis leads to the formation of the intersegmental vessels (ISVs) of the trunk in teleost zebrafish (Danio rerio) embryo. Here we describe experimental procedures, including in vivo observation of transgenic zebrafish embryo and whole mount in situ hybridization, to investigate ISV development in zebrafish embryos and assess the effect of antiangiogenic compounds on these vessels.
authors.affiliation: Department of Molecular and Translational Medicine, Experimental Oncology and Immunology, School of Medicine, University of Brescia, Brescia, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Tobia
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Gariano
authors.forename: Jessica
authors.initials: J
authors.lastname: Guerra
authors.forename: Marco
authors.initials: M
authors.lastname: Presta
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001808:Blood Vessels / Q000196:embryology* / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000098:blood supply*; D017403:In Situ Hybridization / Q000379:methods*; D018919:Neovascularization, Physiologic; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics