title: Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies.
abstract: Electrospun silk fibroin nanofibrous scaffolds (ESFNSs) were successfully prepared by electrospinning of various Bombyx mori silk fibroin concentrations (10, 12, and 14% in formic acid). After characterizing the purified silk fibroin, the morphology, porosity, fibers' diameter, and uniformity of the prepared scaffolds were examined in detail. In addition, biological responses such as effects on bone marrow cell viability, cytotoxicity, and cell adhesion were evaluated in vitro. Biocompatibility and bioactivity properties of the ESFNSs were evaluated in vitro and in vivo by cell culturing and subcutaneous implantation in rat models for 7 and 28 days, respectively. According to the obtained results, no beaded fibers were seen in any of the prepared scaffolds, whereas ESFNS-10% provided more uniformity and porosity with nanoscaled fibers (90 ± 0.021 nm). Furthermore, the scaffolds also showed good cell adhesion and spreading (68.7 ± 11.8 and 7.6 ± 3.3 total length and width, respectively) with no detectable effect on cell viability and cytotoxicity. The in vivo biocompatibility evaluation indicated that the scaffolds did not stimulate detectable cellular inflammatory response (lymphocytes) and increased the total cell number (cellularity) in the implantation area. Furthermore, the results suggest the potential use of the prepared ESFNS-10% bone marrow cell constructs in direct implantation for tissue engineering applications.
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mazaher
authors.initials: M
authors.lastname: Gholipourmalekabadi
authors.affiliation: Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), Tehran, Iran.
authors.forename: Masoud
authors.identifier: http://orcid.org/0000-0002-0232-352X
authors.initials: M
authors.lastname: Mozafari
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Bandehpour
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Sameni
authors.affiliation: Biologics Research, Biotechnology Center of Excellence, Janssen R&D, LLC, Pharmaceutical Companies of Johnson & Johnson, Spring House, PA, USA.|National Biotechnology & Pharmaceutical Association, Chicago, IL, USA.
authors.forename: Hector Hugo
authors.initials: HH
authors.lastname: Caicedo
authors.affiliation: Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Mehdipour
authors.affiliation: Department of Anatomy and Cell Biology, Cellular and Molecular Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Hatef Ghasemi
authors.initials: HG
authors.lastname: Hamidabadi
authors.affiliation: Department of Tissue Engineering & Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.|Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Samadikuchaksaraei
authors.affiliation: Biotechnology Department, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Ghanbarian
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D012831:Bombyx; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004560:Electricity; D005349:Fibroins / Q000737:chemistry* / Q000494:pharmacology*; D005561:Formates / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D016062:Porosity; D051381:Rats
chemical_list: D000890:Anti-Infective Agents; D001672:Biocompatible Materials; D005561:Formates; C030544:formic acid; D005349:Fibroins
keywords: bone marrow adherent cells; electrospinning; nanofibrous scaffold; silk fibroin; tissue engineering