title: The dengue virus conceals double-stranded RNA in the intracellular membrane to escape from an interferon response.
abstract: The dengue virus (DENV) circulates between humans and mosquitoes and requires no other mammals or birds for its maintenance in nature. The virus is well-adapted to humans, as reflected by high-level viraemia in patients. To investigate its high adaptability, the DENV induction of host type-I interferon (IFN) was assessed in vitro in human-derived HeLa cells and compared with that induced by the Japanese encephalitis virus (JEV), a closely related arbovirus that generally exhibits low viraemia in humans. A sustained viral spread with a poor IFN induction was observed in the DENV-infected cells, whereas the JEV infection resulted in a self-limiting and abortive infection with a high IFN induction. There was no difference between DENV and JEV double-stranded RNA (dsRNA) as IFN inducers. Instead, the dsRNA was poorly exposed in the cytosol as late as 48 h post-infection (p.i.), despite the high level of DENV replication in the infected cells. In contrast, the JEV-derived dsRNA appeared in the cytosol as early as 24 h p.i. Our results provided evidence for the first time in DENV, that concealing dsRNA in the intracellular membrane diminishes the effect of the host defence mechanism, a strategy that differs from an active suppression of IFN activity.
authors.affiliation: 1] Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan [2] Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Leo
authors.initials: L
authors.lastname: Uchida
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Lyre Anni
authors.initials: LA
authors.lastname: Espada-Murao
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takamatsu
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Okamoto
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayasaka
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Fuxun
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nabeshima
authors.affiliation: 1] Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan [2] Department of Molecular Epidemiology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Corazon C
authors.initials: CC
authors.lastname: Buerano
authors.affiliation: Department of Virology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Morita
mesh_terms: D003716:Dengue Virus / Q000276:immunology* / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000276:immunology / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057131:Immune Evasion; D007370:Interferon Type I / Q000276:immunology* / Q000378:metabolism; D007425:Intracellular Membranes / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D012330:RNA, Double-Stranded / Q000276:immunology* / Q000378:metabolism; D012367:RNA, Viral / Q000276:immunology* / Q000378:metabolism
chemical_list: D007370:Interferon Type I; D012330:RNA, Double-Stranded; D012367:RNA, Viral