title: Influence of membrane lipid composition on flavonoid-membrane interactions: Implications on their biological activity.
abstract: The membrane interactions and localization of flavonoids play a vital role in altering membrane-mediated cell signaling cascades as well as influence the pharmacological activities such as anti-tumour, anti-microbial and anti-oxidant properties of flavonoids. Various techniques have been used to investigate the membrane interaction of flavonoids. These include partition coefficient, fluorescence anisotropy, differential scanning calorimetry, NMR spectroscopy, electrophysiological methods and molecular dynamics simulations. Each technique will provide specific information about either alteration of membrane fluidity or localization of flavonoids within the lipid bilayer. Apart from the diverse techniques employed, the concentrations of flavonoids and lipid membrane composition employed in various studies reported in literature also are different and together these variables contribute to diverse findings that sometimes contradict each other. This review highlights different techniques employed to investigate the membrane interaction of flavonoids with special emphasis on erythrocyte model membrane systems and their significance in understanding the nature and extent of flavonoid-membrane interactions. We also attempt to correlate the membrane localization and alteration in membrane fluidity with the biological activities of flavonoids such as anti-oxidant, anti-cancer and anti-microbial properties.
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Stalin
authors.initials: S
authors.lastname: Selvaraj
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Sridharan
authors.initials: S
authors.lastname: Krishnaswamy
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Venkappayya
authors.initials: V
authors.lastname: Devashya
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials, School of Chemical & Biotechnology, SASTRA University, Thanjavur 613 401, India. Electronic address: umakrishnan@sastra.edu.
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
mesh_terms: D002152:Calorimetry, Differential Scanning / Q000379:methods; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism*; D004594:Electrophysiology / Q000379:methods; D004912:Erythrocytes; D005419:Flavonoids / Q000378:metabolism*; D005454:Fluorescence Polarization / Q000379:methods; D006801:Humans; D008081:Liposomes / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000378:metabolism*; D056004:Molecular Dynamics Simulation
chemical_list: D005419:Flavonoids; D008081:Liposomes; D008563:Membrane Lipids
keywords: Anti-cancer; Anti-oxidant; DSC; Flavonoid–membrane interactions; Fluorescence anisotropy membrane fluidity; NMR