title: New 9,19-cycloartenol glycosides isolated from the roots of Cimicifuga simplex and their anti-inflammatory effects.
abstract: Two new cycloartenol triterpene saponins, 3β,16α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24R,25-epoxy-cycloartane-3-O-β-D-galactopyranoside (1), 3β,16α-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24R,25-epoxy-cycloartane-7-ene-3-O-β-D-xylopyranoside (2), were isolated from the ethyl acetate soluble fraction of the roots of Cimicifuga simplex Wormsk. Their structures were established by detailed spectroscopic analysis, including extensive 2D NMR data. Their anti-proinflammatory activities were also carried out by LPS-stimulated IL-6, IL-23 and TNF-α genes expression in RAW cells in vitro using Q-PCR method.
authors.affiliation: Key Laboratory of Chinese Material Medica, Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China; Department of Microbiology, Immunology & Molecular Genetics, University of California Los Angeles, Los Angeles, CA 90025, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Su
authors.affiliation: Key Laboratory of Chinese Material Medica, Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Lun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Chinese Material Medica, Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China; The University of Sydney, NSW 2006, Australia. Electronic address: 4865238@qq.com.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Material Medica, Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Bingyou
authors.initials: B
authors.lastname: Yang
authors.affiliation: Key Laboratory of Chinese Material Medica, Department of Pharmacology, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China. Electronic address: suyanggo@hotmail.com.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D031885:Cimicifuga / Q000737:chemistry*; D006027:Glycosides / Q000302:isolation & purification* / Q000494:pharmacology*; D053759:Interleukin-23 / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D010840:Phytosterols / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C000599939:3,16-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24,25-epoxycycloartane-3-O-galactopyranoside; C000599940:3,16-dihydroxy-12-acetoxy-16,22-cyclo-23-ketone-24,25-epoxycycloartane-7-ene-3-O-xylopyranoside; D000893:Anti-Inflammatory Agents; D006027:Glycosides; D053759:Interleukin-23; D015850:Interleukin-6; D010840:Phytosterols; D012503:Saponins; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; C100089:cycloartenol
keywords: 16,22-Cyclo-23-ketone type of cycloartane glycosides; Anti-inflammatory effect in vitro; The roots of Cimicifuga simplex Wormsk