title: The effect of agomelatine on oxidative stress induced with ischemia/reperfusion in rat ovaries.
abstract: BACKGROUND
  Ovarian ischemia and reperfusion can lead to serious and irreversible health problems.
  
  
  OBJECTIVE
  The aim of this study is to investigate the protective effect of agomelatine against ovarian ischemia/ /reperfusion injury in rats using biochemical methods.
  
  
  METHODS
  Thirty female rats were divided into three groups (the number of animals in each group = 10), a control group in which ischemia/reperfusion was established (IRC), an ischemia/reperfusion + agomelatine (IRA) group and a healthy group given a sham operation (SG). Total glutathione (tGSH) and malondialdehyde (MDA) levels and glutathione peroxidase.
  
  
  RESULTS
  Biochemical results revealed MDA levels of 19.1 ± 2.03, 5.8 ± 1.5 and 5.5 ± 1.4 µmol/g protein in ovarian tissue in the IRC, IRA and SG groups, respectively. MPO activity in the IRC, IRA and SG groups was 7.87 ± 2.7, 4.0 ± 2.0 and 3.0 ± 1.0 U/g protein, respectively. tGSH levels were 1.87 ± 1.13, 4.37 ± 1.4 and 5.87 ± 1.64 nmol/g protein, respectively. GPx activity in the IRC, IRA and SG groups was 7.37 ± 1.68, 18.6 ± 3 and 17.75 ± 3.2 U/g protein, and SOD activity 31.1 ± 2.9, 45.3 ± 3.7 and 54 ± 4.2 U/g protein, respectively. The level of 8-OH/ /Gua, a product of DNA damage, was 2.18 ± 0.2 pmol/L in the IRC group, 1.28 ± 0.2 pmol/L in the IRA group and 0.93 ± 0.01 pmol/L in the SG group.
  
  
  CONCLUSIONS
  Agomelatine prevented ovarian ischemia/reperfusion injury.
authors.affiliation: Obstetrics and Gynecology, Sorgun State Hospital, Yozgat, Turkey.
authors.forename: Omer E
authors.initials: OE
authors.lastname: Yapca
authors.affiliation: Department of Obstetrics and Gynecology, Ataturk University, Erzurum, Turkey.
authors.forename: Bunyamin
authors.initials: B
authors.lastname: Borekci
authors.affiliation: Department of Pediatrics, Ataturk University, Erzurum, Turkey.
authors.forename: Mehmet I
authors.initials: MI
authors.lastname: Turan
authors.affiliation: Department of Biochemistry, Ataturk University, Erzurum, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Gulapoglu