title: Lack of centrioles and primary cilia in STIL(-/-) mouse embryos.
abstract: Although most animal cells contain centrosomes, consisting of a pair of centrioles, their precise contribution to cell division and embryonic development is unclear. Genetic ablation of STIL, an essential component of the centriole replication machinery in mammalian cells, causes embryonic lethality in mice around mid gestation associated with defective Hedgehog signaling. Here, we describe, by focused ion beam scanning electron microscopy, that STIL(-/-) mouse embryos do not contain centrioles or primary cilia, suggesting that these organelles are not essential for mammalian development until mid gestation. We further show that the lack of primary cilia explains the absence of Hedgehog signaling in STIL(-/-) cells. Exogenous re-expression of STIL or STIL microcephaly mutants compatible with human survival, induced non-templated, de novo generation of centrioles in STIL(-/-) cells. Thus, while the abscence of centrioles is compatible with mammalian gastrulation, lack of centrioles and primary cilia impairs Hedgehog signaling and further embryonic development.
authors.affiliation: a Sheba Cancer Research Center and the Edmond and Lily Safra Children's Hospital; Sheba Medical Center ; Tel-Hashomer, Ramat Gan , Israel.
authors.forename: Ahuvit
authors.initials: A
authors.lastname: David
authors.forename: Fengying
authors.initials: F
authors.lastname: Liu
authors.forename: Alexandra
authors.initials: A
authors.lastname: Tibelius
authors.forename: Julia
authors.initials: J
authors.lastname: Vulprecht
authors.forename: Diana
authors.initials: D
authors.lastname: Wald
authors.forename: Ulrike
authors.initials: U
authors.lastname: Rothermel
authors.forename: Reut
authors.initials: R
authors.lastname: Ohana
authors.forename: Alexander
authors.initials: A
authors.lastname: Seitel
authors.forename: Jasmin
authors.initials: J
authors.lastname: Metzger
authors.forename: Ruth
authors.initials: R
authors.lastname: Ashery-Padan
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Meinzer
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Gröne
authors.forename: Shai
authors.initials: S
authors.lastname: Izraeli
authors.forename: Alwin
authors.initials: A
authors.lastname: Krämer
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000172:deficiency* / Q000378:metabolism; D002502:Centrioles / Q000378:metabolism* / Q000648:ultrastructure; D002923:Cilia / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D053823:Hedgehog Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008831:Microcephaly / Q000473:pathology; D022101:Microtubule-Organizing Center / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000172:deficiency* / Q000378:metabolism; D015398:Signal Transduction; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D053823:Hedgehog Proteins; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; C465570:Shh protein, mouse; C489366:Tal1 protein, mouse
keywords: CDK6, cyclin-dependent kinase 6; CEP, centrosomal protein; COILEDX, coiled-coil domain deletion; E, embryonic day; FIB/SEM, focused ion beam scanning electron microscopy; MCPH, autosomal recessive primary microcephaly; MEFs, mouse embryonic fibroblasts; MTOC, microtubule organizing center; PLK4, polo kinase 4; SHH, sonic hedgehog; STAN, STIL/ANA2; STANX, STAN domain deletion; STIL; STIL, SCL/TAL1 interrupting locus; centriole; centrosome; electron microscopy; embryo; microcephaly; nm, nanometer; siRNA, small interfering RNA