title: In vitro adhesion of oral Candida dubliniensis isolates to acrylic denture surfaces following brief exposure to sub-cidal concentrations of polyenes, azoles and chlorhexidine.
abstract: OBJECTIVE
  We aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral Candida dubliniensis isolates to the surface of acrylic dentures.
  
  
  METHODS
  After determining the minimum inhibitory concentration of each drug, 20 oral isolates of C. dubliniensis were exposed to sub-cidal concentrations of the drugs for 1 h. The drugs were then removed by dilution, and the adhesion of the isolates to denture acrylic strips was assessed by an in vitro adhesion assay.
  
  
  RESULTS
  Compared to the controls, exposure to nystatin, amphotericin B, ketoconazole, fluconazole and chlorhexidine gluconate suppressed the ability of C. dubliniensis isolates to adhere to acrylic denture surfaces with a reduction of 74.68, 74.27, 57.31, 44.57 and 56.53% (p < 0.001 for all drugs), respectively.
  
  
  CONCLUSIONS
  Brief exposure to sub-cidal concentrations of anti-mycotics suppressed the adhesion of C. dubliniensis oral isolates to acrylic denture surfaces.
authors.affiliation: Departments of Bioclinical, Diagnostics and Restorative Sciences, Faculty of Dentistry, Kuwait University, Jabriya, Kuwait.
authors.forename: Arjuna N B
authors.initials: AN
authors.lastname: Ellepola
authors.forename: Bobby K
authors.initials: BK
authors.lastname: Joseph
authors.forename: Yacoub
authors.initials: Y
authors.lastname: Altarakemah
authors.forename: Lakshman P
authors.initials: LP
authors.lastname: Samaranayake
authors.forename: Sukumaran
authors.initials: S
authors.lastname: Anil
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hashem
authors.forename: Zia U
authors.initials: ZU
authors.lastname: Khan
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D001393:Azoles / Q000008:administration & dosage / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D002710:Chlorhexidine / Q000008:administration & dosage / Q000494:pharmacology*; D003740:Dental Clinics; D003778:Dentures / Q000382:microbiology*; D006801:Humans; D007730:Kuwait; D008826:Microbial Sensitivity Tests; D011090:Polyenes / Q000008:administration & dosage / Q000494:pharmacology*; D019904:Polymethyl Methacrylate
chemical_list: D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D001393:Azoles; D011090:Polyenes; D019904:Polymethyl Methacrylate; D002710:Chlorhexidine