title: Arg-vasopressin facilitates calcium channel currents in osteoblasts.
abstract: The hypothalamic nonapeptide and neurohypophyseal hormone arg-vasopressin (AVP), also known as antidiuretic hormone, is best known for its effects on water reabsorption in kidney. Osteoblasts play a major role in bone formation, employing intracellular Ca(2+) as a second messenger to modulate hormonal responses and as a cofactor for mineralization. Voltage-dependent Ca(2+) channels (VDCCs) mediate the influx of Ca(2+) in response to membrane depolarization. The purpose of this study was to investigate the effects of AVP on VDCC currents in osteoblasts using a patch-clamp recording method. An application of 1μM AVP facilitated VDCC currents in osteoblasts. To our knowledge, the data presented here demonstrate for the first time that AVP facilitates VDCCs in osteoblasts.
authors.affiliation: Department of Clinical Oral Health Science, Tokyo Dental College.
authors.forename: Sohei
authors.initials: S
authors.lastname: Hosokawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Endoh
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Tadashi
authors.initials: T
authors.lastname: Furuya
authors.forename: Toru
authors.initials: T
authors.lastname: Sato
authors.forename: Masakazu
authors.initials: M
authors.lastname: Tazaki
authors.forename: Kenji
authors.initials: K
authors.lastname: Sueishi
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Yamashita
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001127:Arginine Vasopressin / Q000502:physiology*; D015220:Calcium Channels / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology*; D018929:Cell Culture Techniques; D015640:Ion Channel Gating / Q000502:physiology; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015290:Second Messenger Systems / Q000502:physiology
chemical_list: D015220:Calcium Channels; D001127:Arginine Vasopressin