title: Crystallization and preliminary X-ray study of biosynthetic alanine racemase from Pseudomonas aeruginosa PAO1.
abstract: Biosynthetic alanine racemase (AlrPA) from Pseudomonas aeruginosa PAO1 carrying a His6 tag was expressed in Escherichia coli BL21 (DE3) cells and purified by Ni(2+)-chelating affinity and anion-exchange chromatography for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K in a solution consisting of 4%(v/v) Tacsimate pH 5.0, 14%(w/v) polyethylene glycol 3350 with a protein concentration of 8 mg ml(-1). The crystal diffracted to 2.76 Å resolution and belonged to the orthorhombic space group P212121, with unit-cell parameters a = 74.12, b = 76.97, c = 154.80 Å, α = β = γ = 90°.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
authors.forename: Guofang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Xu
authors.affiliation: Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
authors.forename: Zhaona
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Tianjin International Joint Academy of Biomedicine, Tianjin 300457, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ju
mesh_terms: D000411:Alanine Racemase / Q000737:chemistry*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D017931:DNA Primers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D000411:Alanine Racemase
keywords: Pseudomonas aeruginosa; biosynthetic alanine racemase