title: Glycan moiety of flagellin in Acidovorax avenae K1 prevents the recognition by rice that causes the induction of immune responses.
abstract: Abstract Recognition of pathogen-associated molecular patterns (PAMPs) such as flagellin, a main component of the bacterial flagellum, constitutes the first layer of plant immunity and is referred to as PAMP-triggered immunity (PTI). The rice avirulent N1141 strain of gram-negative phytopathogenic bacterium, Acidovorax avenae, induces PTI including H2O2 generation, while flagellin from the rice virulent K1 strain of A. avenae does not induce these immune responses. Mass spectrometry analyses revealed that total 1,600-Da and 2,150-Da of glycan residues were present on the flagellins from N1141 and K1, respectively. A deglycosylated K1 flagellin induced immune responses in the same manner as N1141 flagellin, suggesting that the glycan in K1 flagellin prevent epitope recognition in rice. We identified three genes in K1 flagella operon, which regulate structural modification of glycan in K1 flagellin. The immature glycan-attached flagellin from three genes deletion mutant, KΔ3FG, induced H2O2 generation in cultured rice cells, whereas the K1 mature-type flagellin did not cause a detectable increase in H2O2. The data indicate that the immature glycan of flagellin from KΔ3FG cannot prevent the epitope recognition in rice.
authors.affiliation: a Graduate School of Bio-Science; Nagahama Institute of Bio-Science and Technology; Tamura Nagahama , Shiga , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Hirai
authors.forename: Ryota
authors.initials: R
authors.lastname: Takai
authors.forename: Machiko
authors.initials: M
authors.lastname: Kondo
authors.forename: Takehito
authors.initials: T
authors.lastname: Furukawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hishiki
authors.forename: Seiji
authors.initials: S
authors.lastname: Takayama
authors.forename: Fang-Sik
authors.initials: FS
authors.lastname: Che
mesh_terms: D042621:Comamonadaceae / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005408:Flagellin / Q000276:immunology*; D017343:Genes, Plant; D006031:Glycosylation; D012275:Oryza / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D057865:Plant Immunity; D011134:Polysaccharides / Q000276:immunology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000939:Epitopes; D011134:Polysaccharides; D005408:Flagellin
keywords: cultured rice cells; flagellin; glycan; plant immune responses; reactive oxygen species generation