title: Brain-derived neurotrophic factor (BDNF) expression in normal and regenerating olfactory epithelium of Xenopus laevis.
abstract: Olfactory epithelium has the capability to continuously regenerate olfactory receptor neurons throughout life. Adult neurogenesis results from proliferation and differentiation of neural stem cells, and consequently, olfactory neuroepithelium offers an excellent opportunity to study neural regeneration and the factors involved in the maintenance and regeneration of all their cell types. We analyzed the expression of BDNF in the olfactory system under normal physiological conditions as well as during a massive regeneration induced by chemical destruction of the olfactory epithelium in Xenopus laevis larvae. We described the expression and presence of BDNF in the olfactory epithelium and bulb. In normal physiological conditions, sustentacular (glial) cells and a few scattered basal (stem) cells express BDNF in the olfactory epithelium as well as the granular cells in the olfactory bulb. Moreover, during massive regeneration, we demonstrated a drastic increase in basal cells expressing BDNF as well as an increase in BDNF in the olfactory bulb and nerve. Together these results suggest an important role of BDNF in the maintenance and regeneration of the olfactory system.
authors.affiliation: Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Jimena Laura
authors.initials: JL
authors.lastname: Frontera
authors.affiliation: Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Ailen Soledad
authors.initials: AS
authors.lastname: Cervino
authors.affiliation: Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina; Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Lucas David
authors.initials: LD
authors.lastname: Jungblut
authors.affiliation: Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina; Laboratorio de Biología del Desarrollo, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address: dante@bg.fcen.uba.ar.
authors.forename: Dante Agustín
authors.initials: DA
authors.lastname: Paz
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology; D055495:Neurogenesis / Q000502:physiology; D009830:Olfactory Bulb / Q000473:pathology* / Q000503:physiopathology*; D009831:Olfactory Mucosa / Q000473:pathology* / Q000503:physiopathology*; D014982:Xenopus laevis
chemical_list: D019208:Brain-Derived Neurotrophic Factor
keywords: Neural stem cells; Neurogenesis; Neurotrophins; Olfactory bulb; Olfactory neurons; Sustentacular cells