title: DNA origami nanoneedles on freestanding lipid membranes as a tool to observe isotropic-nematic transition in two dimensions.
abstract: We introduce a simple experimental system to study dynamics of needle-like nanoobjects in two dimensions (2D) as a function of their surface density close to the isotropic-nematic transition. Using fluorescence correlation spectroscopy, we find that translational and rotational diffusion of rigid DNA origami nanoneedles bound to freestanding lipid membranes is strongly suppressed upon an increase in the surface particle density. Our experimental observations show a good agreement with results of Monte Carlo simulations of Brownian hard needles in 2D.
authors.affiliation: Laboratory of Membrane Biochemistry, Paul Langerhans Institute, Technische Universität Dresden , Fetscherstraße 74, 01307 Dresden, Germany.
authors.forename: Aleksander
authors.initials: A
authors.lastname: Czogalla
authors.forename: Dominik J
authors.initials: DJ
authors.lastname: Kauert
authors.forename: Ralf
authors.initials: R
authors.lastname: Seidel
authors.forename: Petra
authors.initials: P
authors.lastname: Schwille
authors.forename: Eugene P
authors.initials: EP
authors.lastname: Petrov
mesh_terms: D004247:DNA / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008956:Models, Chemical; D009010:Monte Carlo Method; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D008051:Lipid Bilayers; D004247:DNA
keywords: DNA origami; fluorescence correlation spectroscopy; isotropic−nematic transition; lipid membrane; rotational diffusion; translational diffusion