title: GmPGIP3 enhanced resistance to both take-all and common root rot diseases in transgenic wheat.
abstract: Take-all (caused by the fungal pathogen Gaeumannomyces graminis var. tritici, Ggt) and common root rot (caused by Bipolaris sorokiniana) are devastating root diseases of wheat (Triticum aestivum L.). Development of resistant wheat cultivars has been a challenge since no resistant wheat accession is available. GmPGIP3, one member of polygalacturonase-inhibiting protein (PGIP) family in soybean (Glycine max), exhibited inhibition activity against fungal endopolygalacturonases (PGs) in vitro. In this study, the GmPGIP3 transgenic wheat plants were generated and used to assess the effectiveness of GmPGIP3 in protecting wheat from the infection of Ggt and B. sorokiniana. Four independent transgenic lines were identified by genomic PCR, Southern blot, and reverse transcription PCR (RT-PCR). The introduced GmPGIP3 was integrated into the genomes of these transgenic lines and could be expressed. The expressing GmPGIP3 protein in these transgenic wheat lines could inhibit the PGs produced by Ggt and B. sorokiniana. The disease response assessments postinoculation showed that the GmPGIP3-expressing transgenic wheat lines displayed significantly enhanced resistance to both take-all and common root rot diseases caused by the infection of Ggt and B. sorokiniana. These data suggested that GmPGIP3 is an attractive gene resource in improving resistance to both take-all and common root rot diseases in wheat.
authors.affiliation: College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China.
authors.forename: Aiyun
authors.initials: A
authors.lastname: Wang
authors.forename: Xuening
authors.initials: X
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Rong
authors.forename: Liang
authors.initials: L
authors.lastname: Dang
authors.forename: Li-Pu
authors.initials: LP
authors.lastname: Du
authors.forename: Lin
authors.initials: L
authors.lastname: Qi
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Xu
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Shao
authors.forename: Zengyan
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D001203:Ascomycota; D060467:Disease Resistance / Q000235:genetics*; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D014908:Triticum / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*
chemical_list: C081479:PGIP protein, plant; D010940:Plant Proteins