title: Functional graphene-gold nano-composite fabricated electrochemical biosensor for direct and rapid detection of bisphenol A.
abstract: In this research, the graphene with excellent dispersity is prepared successfully by introducing gold nanoparticle to separate the individual sheets. Various techniques are adopted to characterize the prepared graphene and graphene-gold nanoparticle composite materials. This fabricated new composite material is used as the support material to construct a novel tyrosinase based biosensor for detection of bisphenol A (BPA). The electrochemical performances of the proposed new enzyme biosensor were investigated by differential pulse voltammetry (DPV) method. The proposed biosensor exhibited excellent performance for BPA determination with a wide linear range (2.5×10(-3)-3.0 μM), a highly reproducible response (RSD of 2.7%), low interferences and long-term stability. And more importantly, the calculated detection limit of the proposed biosensor was as low as 1 nM. Compared with other detection methods, this graphene-gold nanoparticle composite based tyrosinase biosensor is proved to be a promising and reliable tool for rapid detection of BPA for on-site analysis of emergency BPA related pollution affairs.
authors.affiliation: Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China; Department of Food Science & Nutrition of Ginling College, Nanjing Normal University, Nanjing 210097, China. Electronic address: daodongpan@163.com.
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.affiliation: Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China.
authors.forename: Hangzhen
authors.initials: H
authors.lastname: Lan
authors.affiliation: Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China.
authors.forename: Yangying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Food Science & Technology of Marine Science School, Ningbo University, Ningbo 315211, China.
authors.forename: Huiju
authors.initials: H
authors.lastname: Gao
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D008958:Models, Molecular; D008968:Molecular Conformation; D014442:Monophenol Monooxygenase / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D010636:Phenols / Q000032:analysis* / Q000737:chemistry; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D006046:Gold; D006108:Graphite; D014442:Monophenol Monooxygenase; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Differential pulse voltammetry; Graphene-gold nanoparticle; Tyrosinase based biosensor