title: Faecal metabolomic fingerprint after moderate consumption of red wine by healthy subjects.
abstract: Faecal metabolome contains information on the metabolites found in the intestine, from which knowledge about the metabolic function of the gut microbiota can be obtained. Changes in the metabolomic profile of faeces reflect, among others, changes in the composition and activity of the intestinal microorganisms. In an effort to improve our understanding of the biological effects that phenolic compounds (including red wine polyphenols) exert at the gut level, in this foodomic study we have undertaken a metabolome characterization of human faeces after moderate consumption of red wine by healthy subjects for 4 weeks. Namely, a nontargeted metabolomic approach based on the use of UHPLC-TOF MS was developed to achieve the maximum metabolite information on 82 human faecal samples. After data processing and statistical analysis, 37 metabolites were related to wine intake, from which 20 could be tentatively or completely identified, including the following: (A) wine compounds, (B) microbial-derived metabolites of wine polyphenols, and (C) endogenous metabolites and/or others derived from other nutrient pathways. After wine consumption, faecal metabolome was fortified in flavan-3-ols metabolites. Also, of relevance was the down regulation of xanthine and bilirubin-derived metabolites such as urobilinogen and stercobilin after moderate wine consumption. As far as we know, this is the first study of the faecal metabolome after wine intake.
authors.affiliation: Institute of Food Science Research (CIAL), CSIC-UAM , C/Nicolás Cabrera, 9. CEI UAM+CSIC. Campus de Cantoblanco, 28049 Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez-Girón
authors.forename: Clara
authors.initials: C
authors.lastname: Ibáñez
authors.forename: Alejandro
authors.initials: A
authors.lastname: Cifuentes
authors.forename: Carolina
authors.initials: C
authors.lastname: Simó
authors.forename: Irene
authors.initials: I
authors.lastname: Muñoz-González
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Martín-Álvarez
authors.forename: Begoña
authors.initials: B
authors.lastname: Bartolomé
authors.forename: M Victoria
authors.initials: MV
authors.lastname: Moreno-Arribas
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D055442:Metabolome / Q000502:physiology*; D055432:Metabolomics; D008875:Middle Aged; D059808:Polyphenols / Q000032:analysis / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014920:Wine; D055815:Young Adult
chemical_list: D059808:Polyphenols
keywords: LC-TOF MS; faecal metabolome; metabolomics; wine polyphenols