title: [Different ratio of hormone effects on Fritillaria cirrhosa bulb induction].
abstract: OBJECTIVE
  To study the effects of different hormone induced bulb of Fritillaria cirrhosa seedling leaves, in order to provide an effective way to expant Fritillaria cirrhosa source.
  
  
  METHODS
  Fritillaria cirrhosa seedling leaves were used as explants, orthogonal test was carried out to study the medium consisting of 6-BA, NAA, 2, 4-D and KT, and proliferation induced by direct bulb. Induction rate and average number of bulb producing was treated as the assessment indices, and the content of alkaloid of bulb was determined.
  
  
  RESULTS
  The best medium formula for Fritillaria cirrhosa tissue culture seedling was MS+6-BA 2.0 mg/L +NAA 1.0mg/L +KT 1.0mg/L, the bulb induction rate was 83.37% and the average number of bulb was 17.27. The alkaloid content of induced bulb was 0.389%, which was 1.64 times that of wild Fritillaria cirrhosa bulb.
  
  
  CONCLUSIONS
  This study provides a simple and rapid mehod for the production of tissue culture of Fritillaria cirrhosa bulb. It is useful for reasonable development and utilization of Fritillaria cirrhosa resources, as well as improvement of the quality of medicinal materials.
authors.forename: Yue-hua
authors.initials: YH
authors.lastname: Wang
authors.forename: Cui-ping
authors.initials: CP
authors.lastname: Guo
authors.forename: Shi-hong
authors.initials: SH
authors.lastname: He
authors.forename: Ming-shu
authors.initials: MS
authors.lastname: Jiang
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Xiao-rong
authors.initials: XR
authors.lastname: Wang
mesh_terms: D000470:Alkaloids; D031410:Fritillaria / Q000187:drug effects* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018517:Plant Roots
chemical_list: D000470:Alkaloids; D010937:Plant Growth Regulators