title: Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry.
abstract: In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract.
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Ticiana Silva
authors.initials: TS
authors.lastname: Rocha
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Ana Angelita Sampaio
authors.initials: AA
authors.lastname: Baptista
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Tais Cremasco
authors.initials: TC
authors.lastname: Donato
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Elisane Lenita
authors.initials: EL
authors.lastname: Milbradt
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Adriano Sakai
authors.initials: AS
authors.lastname: Okamoto
authors.affiliation: Laboratório de Ornitopatologia Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista BotucatuSP Brazil Laboratório de Ornitopatologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Raphael Lucio
authors.initials: RL
authors.lastname: Andreatti Filho
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D007413:Intestinal Mucosa / Q000382:microbiology; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial
keywords: Lactobacillus; identification; intestinal adhesion