title: Selective recognition of Glutamate based on fluorescence enhancement of graphene quantum dot.
abstract: Graphene quantum dots (GQDs) have successfully been utilized as an efficient nano-sized fluorescence chemosensor to detect selectively Glutamate (Glu) in Tris-HCl buffer solution (pH=9). The fluorescence emission spectrum of graphene quantum dots was at about 430nm. The study showed that fluorescence intensity of the quantum dot gradually enhanced with increase in concentration of Glutamate and any change in fluorescence intensity was directly proportional to the concentration of Glutamate. Under optimum conditions, the linear range for the detection of Glutamate was 1.6×10(-7)M to 1.0×10(-5)M with a detection limit of 5.2×10(-8)M. The sensor showed high selectivity toward Glutamate in comparison with other amino acids.
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran. Electronic address: smhosseini@khayam.ut.ac.ir.
authors.forename: Morteza
authors.initials: M
authors.lastname: Hosseini
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Khabbaz
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.
authors.forename: Amin Shiralizadeh
authors.initials: AS
authors.lastname: Dezfoli
authors.affiliation: Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran; Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Ganjali
authors.affiliation: Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran; Payame Noor University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dadmehr
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D018698:Glutamic Acid / Q000032:analysis* / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D018698:Glutamic Acid; D006108:Graphite
keywords: Amino acid; Fluorescence enhancement; Glutamate; Graphene; Quantum dot