title: Characterization of M-laurdan, a versatile probe to explore order in lipid membranes.
abstract: Microdomains corresponding to localized partition of lipids between ordered and less ordered environments are the subject of intensive investigations, because of their putative participation in modulating cellular responses. One popular approach in the field consists in labelling membranes with solvatochromic fluorescent probes such as laurdan and C-laurdan. In this report, we describe a high-yield procedure for the synthesis of laurdan, C-laurdan and two new fluorophores, called MoC-laurdan and M-laurdan, as well as their extensive photophysical characterization. We find that the latter probe, M-laurdan, is particularly suited to discriminate lipid phases independently of the chemical nature of the lipids, as measured by both fluorescence Generalized Polarization (GP) and anisotropy in large unilamellar vesicles made of various lipid compositions. In addition, staining of live cells with M-laurdan shows a good stability over time without any apparent toxicity, as well as a wider distribution in the various cell compartments than the other probes.
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, Toulouse, F-31077, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Mazeres
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, Toulouse, F-31077, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Joly
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, Toulouse, F-31077, France.
authors.forename: Andre
authors.initials: A
authors.lastname: Lopez
authors.affiliation: CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale), Université de Toulouse, Toulouse, F-31077, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Tardin