title: Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges.
abstract: Although single-cell oil (SCO) has been studied for decades, lipid production from lignocellulosic biomass has received substantial attention only in recent years as biofuel research moves toward producing drop-in fuels. This review gives an overview of the feasibility and challenges that exist in realizing microbial lipid production from lignocellulosic biomass in a biorefinery. The aspects covered here include biorefinery technologies, the microbial oil market, oleaginous microbes, lipid accumulation metabolism, strain development, process configurations, lignocellulosic lipid production, technical hurdles, lipid recovery, and technoeconomics. The lignocellulosic SCO-based biorefinery will be feasible only if a combination of low- and high-value lipids are coproduced, while lignin and protein are upgraded to high-value products.
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, MBI Building, 3815 Technology Boulevard, Lansing, MI 48910, USA. Electronic address: jinmingj@egr.msu.edu.
authors.forename: Mingjie
authors.initials: M
authors.lastname: Jin
authors.affiliation: National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Patricia J
authors.initials: PJ
authors.lastname: Slininger
authors.affiliation: National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Dien
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, MBI Building, 3815 Technology Boulevard, Lansing, MI 48910, USA.
authors.forename: Suresh
authors.initials: S
authors.lastname: Waghmode
authors.affiliation: National Center for Agricultural Utilization Research, USDA-ARS, 1815 North University Street, Peoria, IL 61604, USA.
authors.forename: Bryan R
authors.initials: BR
authors.lastname: Moser
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, MBI Building, 3815 Technology Boulevard, Lansing, MI 48910, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Orjuela
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, MBI Building, 3815 Technology Boulevard, Lansing, MI 48910, USA.
authors.forename: Leonardo da Costa
authors.initials: Lda C
authors.lastname: Sousa
authors.affiliation: Biomass Conversion Research Laboratory (BCRL), Department of Chemical Engineering and Materials Science, Michigan State University, MBI Building, 3815 Technology Boulevard, Lansing, MI 48910, USA. Electronic address: balan@msu.edu.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Balan
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D056804:Biofuels; D018533:Biomass; D053898:Biosynthetic Pathways; D001709:Biotechnology / Q000379:methods*; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism*; D008954:Models, Biological
chemical_list: D056804:Biofuels; D008055:Lipids; D008031:Lignin
keywords: biorefinery; lignocellulosic lipids; microbial lipids; single-cell oil