title: Phage display library screening for identification of interacting protein partners.
abstract: Phage display is a versatile high-throughput screening method employed to understand and improve the chemical biology, be it production of human monoclonal antibodies or identification of interacting protein partners. A majority of cell proteins operate in a concerted fashion either by stable or transient interactions. Such interactions can be mediated by recognition of small amino acid sequence motifs on the protein surface. Phage display can play a crucial role in identification of such motifs. This report describes the use of phage display for the identification of high affinity sequence motifs that could be responsible for interactions with a target (bait) protein.
authors.affiliation: Rieveschl laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, 312 College Dr, Cincinnati, OH, 45221, USA, balasual@ucmail.uc.edu.
authors.forename: Balasubrahmanyam
authors.initials: B
authors.lastname: Addepalli
authors.forename: Suryadevara
authors.initials: S
authors.lastname: Rao
authors.forename: Arthur G
authors.initials: AG
authors.lastname: Hunt
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D057166:High-Throughput Screening Assays; D019151:Peptide Library; D010455:Peptides / Q000378:metabolism*; D026723:Polyadenylation; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D039221:mRNA Cleavage and Polyadenylation Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D019151:Peptide Library; D010455:Peptides; D021122:Protein Subunits; D039221:mRNA Cleavage and Polyadenylation Factors