title: A multifunctional label-free electrochemical impedance biosensor for Hg(2+), adenosine triphosphate and thrombin.
abstract: A multifunctional label-free biosensor for the detection of Hg(2+), adenosine triphosphate and thrombin has been developed based on the changing of the electrochemical impedance spectroscopy (EIS) from the modified electrodes when nucleic acid subunits interacting with different targets. The modified electrode consists of three interaction sections, including DNA with T-T mismatch recognizing Hg(2+) to form T-Hg(2+)-T complex, split DNA chip against ATP, and DNA domin against thrombin to form G-quadruplex. Upon DNA interaction with thrombin or ATP, an increased charge transfer resistance (Rct) had been detected. However, a decreased Rct against Hg(2+) was obtained. The Rct difference (ΔRct) has relationship with the concentration of the different targets, Hg(2+), ATP and thrombin can be selectively detected with the detection limit of 0.03, 0.25, and 0.20 nmol L(-1), respectively. To separately detect the three analytes existing in the same sample, ATP aptamer, G-rich DNA strands and EDTA were applied to mask ATP, Hg(2+) or thrombin separately.
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China. Electronic address: czn@fjirsm.ac.cn.
authors.forename: Zhong-Ning
authors.initials: ZN
authors.lastname: Chen
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000138:chemical synthesis / Q000737:chemistry*; D020137:Base Pair Mismatch; D015374:Biosensing Techniques; D002021:Buffers; D002413:Cations, Divalent; D004247:DNA / Q000737:chemistry*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D054856:G-Quadruplexes; D057230:Limit of Detection; D008628:Mercury / Q000032:analysis*; D012996:Solutions; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D002021:Buffers; D002413:Cations, Divalent; D012996:Solutions; D000255:Adenosine Triphosphate; D004247:DNA; D013917:Thrombin; D008628:Mercury
keywords: Adenosine triphosphate; Biosensor; Electrochemical impedance spectroscopy; Hg(2+); Thrombin