title: Heavy-ion-induced sucrose radicals investigated using EPR and UV spectroscopy.
abstract: The potential use of a sucrose dosimeter for estimating both linear energy transfer (LET) and the absorbed dose of heavy ion and X-ray radiation was investigated. The stable free radicals were produced when sucrose was irradiated with heavy ions, such as helium, carbon, silicon and neon ions, and when the X-ray radiation was similar to the obtained electron paramagnetic resonance (EPR) spectra, which were ∼7 mT wide and composed of several hyperfine structures. In addition, the total spin concentration resulting from heavy-ion irradiation increased linearly as the absorbed dose increased, and decreased logarithmically as the LET increased. These empirical relations imply that the LET at a certain dose can be determined from the spin concentration. For sucrose and alanine, both cross-sections following C-ion irradiation with a 50 Gy dose were ∼1.3 × 10(-12) [μm(2)], taking into account the molecular size of the samples. The values of these cross-sections imply that multiple ionizing particles were involved in the production of stable radicals. Furthermore, UV absorbance at 267 nm of an aqueous solution of irradiated sucrose was found to linearly increase with increasing absorbed dose. Therefore, the EPR and UV results suggest that sucrose can be a useful dosimeter for heavy-ion irradiation.
authors.affiliation: Department of Radiological Life Sciences, Graduate School of Health Sciences, Hirosaki University, 66-1 Hon-cho, Hirosaki, 036-8564, Japan nakagawa@cc.hirosaki-u.ac.jp.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Nakagawa
authors.affiliation: National EPR Center, Institute of Catalysis, Bulgaria Academy of Sciences, Sofia, Bulgaria.
authors.forename: Yordanka
authors.initials: Y
authors.lastname: Karakirova
authors.affiliation: National EPR Center, Institute of Catalysis, Bulgaria Academy of Sciences, Sofia, Bulgaria.
authors.forename: Nicola D
authors.initials: ND
authors.lastname: Yordanov
mesh_terms: D004307:Dose-Response Relationship, Radiation; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals / Q000737:chemistry / Q000528:radiation effects; D020450:Heavy Ions; D008422:Materials Testing; D011829:Radiation Dosage; D011874:Radiometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013395:Sucrose / Q000737:chemistry* / Q000528:radiation effects*
chemical_list: D005609:Free Radicals; D013395:Sucrose
keywords: EPR; UV spectrometry; X-ray; alanine; cross-section; heavy ion; irradiation; radical; sucrose; sugar