title: Occurence and antimicrobial resistance of Staphylococcus aureus and Salmonella spp. in retail fish samples in Turkey.
abstract: The aims of this study were to investigate the presence of Staphylococcus aureus and staphylococcal enterotoxins, as well as Salmonella spp. and to determine the antimicrobial susceptibilities of the isolates from fish samples. A total of 100 fish samples were analysed consisting of 30 anchovy, 35 trout and 35 sea bream. The presence of SEs was detected using ELISA and its genes confirmed by mPCR. Also, S. aureus and Salmonella spp. were detected in 9 (9%) and 5 (5%) samples, respectively. None of the S. aureus isolates had SEs and SEs genes. The resistance rates of the S. aureus isolates to erythromycin, tetracycline, and penicillin G were found to be 33% while Salmonella spp. isolates were resistant to trimethoprim-sulfamethoxazole, gentamicin and neomycine in 20%, 20% and 80%, respectively of the samples. It is of utmost important for public health that retail fish markets need to use hygienic practices in handling and processing operations.
authors.affiliation: University of Erciyes, Department of Food Hygiene and Technology, 38039 Kayseri, Turkey. Electronic address: nertas@erciyes.edu.tr.
authors.forename: Nurhan
authors.initials: N
authors.lastname: Ertas Onmaz
authors.affiliation: University of Erciyes, Department of Microbiology, 38039 Kayseri, Turkey.
authors.forename: Secil
authors.initials: S
authors.lastname: Abay
authors.affiliation: University of Nigde, Bor Vocational School, 51700 Nigde, Turkey.
authors.forename: Fulden
authors.initials: F
authors.lastname: Karadal
authors.affiliation: University of Erciyes, Department of Food Hygiene and Technology, 38039 Kayseri, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Hizlisoy
authors.affiliation: University of Selcuk, Vocational School of Technical Sciences, 42030 Konya, Turkey.
authors.forename: Nihat
authors.initials: N
authors.lastname: Telli
authors.affiliation: University of Erciyes, Department of Food Hygiene and Technology, 38039 Kayseri, Turkey.
authors.forename: Serhat
authors.initials: S
authors.lastname: Al
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D004768:Enterotoxins / Q000032:analysis; D005399:Fishes / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D012475:Salmonella / Q000187:drug effects* / Q000302:isolation & purification; D017747:Seafood / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification; D014421:Turkey
chemical_list: D000900:Anti-Bacterial Agents; D004768:Enterotoxins
keywords: ELISA; Fish; PCR; S. aureus; Salmonella spp.