title: Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors.
abstract: The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO4) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in in vitro Bacopa monnieri shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74 mg g(-1) dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91 mg g(-1) DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45 mg L(-1) of CuSO4 exhibited the highest bacoside content of 8.73 mg g(-1) DW (∼1.42-fold higher) than in control cultures (6.14 mg g(-1) DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in in vitro shoot cultures of this medicinally important herb known for its memory-enhancing properties.
authors.affiliation: a School of Biotechnology, Shri Mata Vaishno Devi University , Katra 182320 , India.
authors.forename: Munish
authors.initials: M
authors.lastname: Sharma
authors.forename: Ashok
authors.initials: A
authors.lastname: Ahuja
authors.forename: Rajinder
authors.initials: R
authors.lastname: Gupta
authors.forename: Sharada
authors.initials: S
authors.lastname: Mallubhotla
mesh_terms: D034681:Bacopa / Q000378:metabolism*; D018533:Biomass; D019327:Copper Sulfate / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D003517:Cyclopentanes / Q000737:chemistry; D054883:Oxylipins / Q000737:chemistry; D018520:Plant Shoots / Q000378:metabolism; D020156:Salicylic Acid / Q000737:chemistry; D012503:Saponins / Q000096:biosynthesis*; D046509:Tissue Culture Techniques; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D003470:Culture Media; D003517:Cyclopentanes; D054883:Oxylipins; D012503:Saponins; D014315:Triterpenes; C011006:jasmonic acid; D019327:Copper Sulfate; D020156:Salicylic Acid
keywords: Brahmi; HPLC; elicitation; secondary metabolite production; shoot biomass