title: Isolation and characterization of canine tumor endothelial cells.
abstract: The present study involved the isolation and characterization of canine tumor endothelial cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma. Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.
authors.affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Izumi
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hoshino
authors.forename: Kenji
authors.initials: K
authors.lastname: Hosoya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takagi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Okumura
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000662:veterinary*; D002478:Cells, Cultured; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D042783:Endothelial Cells / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D008113:Liver Neoplasms / Q000378:metabolism / Q000662:veterinary*; D018239:Seminoma / Q000378:metabolism / Q000662:veterinary*