title: Antioxidant potential is correlated to ω6 / ω3 ratio and Brasfield score in cystic fibrosis children.
abstract: OBJECTIVE
  Oxidative stress is associated with the condition of cystic fibrosis (CF), but no guidelines exist for its assessment or treatment. Our aim was to evaluate a test that measures the blood antioxidant capability in CF children.
  
  
  METHODS
  This antioxidant capability was assessed by the Kit Radicaux Libres (KRL) test in 44 CF children (24 boys). We recorded also anthropometric measures, pulmonary function, CF severity scores, and plasma nutritional and inflammatory parameters (proteins, vitamins, erythrocyte fatty acids, and micronutrients). We performed univariate and multivariate analyses with linear regression models.
  
  
  RESULTS
  The mean age at the first KRL assessment was 12.2 ± 3.8 years. Factors that correlated with decreased antioxidant capacity were mostly related to the severity of pulmonary disease [ forced expiratory volume at 1 second (FEV1), acute exacerbation, and congestion. In multivariate analysis, the correlation between Brasfield score and erythrocyte antioxidant potential remained significant (β = - 0.611, p < 0.001). Among nutritional factors, the ω6/ω3 ratio was significantly correlated to erythrocyte antioxidant potential (β = - 1.213, p = 0.01).
  
  
  CONCLUSIONS
  The blood antioxidant capability, measured by the KRL test, appears to be an interesting biomarker to evaluate oxidative stress in CF. This study suggests that the ω6/ω3 ratio should be regarded as a nutritional marker in antioxidant management in CF children.
authors.affiliation: Service de pédiatrie multidisciplinaire, Hôpital des Enfants de la Timone, Marseille, France.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Fabre
authors.affiliation: Laboratoire de Biochimie nutritionnelle, Institut Fédératif de Biologie, Hôpital Purpan, Toulouse, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Caspar-Bauguil
authors.affiliation: APHM, CHU Timone, Service de santé publique et dinformation médicale, Marseille, France; UMR912, SESSTIM, Université Aix-Marseille, Marseille, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Gaudart
authors.affiliation: APHM, CHU Timone, Service de santé publique et dinformation médicale, Marseille, France; UMR912, SESSTIM, Université Aix-Marseille, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Mancini
authors.affiliation: Laboratoire de Biochimie nutritionnelle, Institut Fédératif de Biologie, Hôpital Purpan, Toulouse, France.
authors.forename: Jésus
authors.initials: J
authors.lastname: Garcia
authors.affiliation: Service de pneumologie, Hôpital des Enfants, CHU Toulouse, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Mittaine
authors.affiliation: Service de pneumologie, Hôpital des Enfants, CHU Toulouse, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Rittié
authors.affiliation: Service de pneumologie, Hôpital des Enfants, CHU Toulouse, France.
authors.forename: François
authors.initials: F
authors.lastname: Brémont
authors.affiliation: Unité de Gastroentérologie, Hépatologie, Nutrition et Diabétologie, Hôpital des Enfants, CHU Toulouse, France, and INSERM U1043, Toulouse, France, and CNRS, U5282, Toulouse, France, and Université de Toulouse, UPS, Centre de Physiopathologie de Toulouse Purpan (CPTP), Toulouse, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Mas
mesh_terms: D000293:Adolescent; D000886:Anthropometry; D000975:Antioxidants / Q000032:analysis*; D015415:Biomarkers / Q000097:blood; D002648:Child; D003550:Cystic Fibrosis / Q000097:blood / Q000503:physiopathology*; D004912:Erythrocytes / Q000737:chemistry; D015525:Fatty Acids, Omega-3 / Q000097:blood*; D043371:Fatty Acids, Omega-6 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D009752:Nutritional Status; D018384:Oxidative Stress; D012129:Respiratory Function Tests; D012189:Retrospective Studies; D012737:Sex Factors
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6
keywords: children; fatty acids; nutritional status; oxidative stress; respiratory system