title: Sex specific response in cholesterol level in zebrafish (Danio rerio) after long-term exposure of difenoconazole.
abstract: Difenoconazole is a widely used triazole fungicide, its extensive application may potentially cause toxic effects on non-target organisms. To investigate the effect of difenoconazole on cholesterol content and related mechanism, adult zebrafish were exposed to environmental related dosage (0.1, 10 and 500 μg/L) difenoconazole. The body weight and hepatic total cholesterol (TCHO) level was tested at 7, 15 and 30 days post exposure (dpe). The expressions of eight cholesterol synthesis genes and one cholesterol metabolism gene were assessed via Quantitative PCR method. The significant decrease of TCHO level in male zebrafish liver was observed at 15 and 30 dpe, which was accompanied by apparent hepatic cholesterol-genesis genes expression decline. In comparison with males, female zebrafish showed different transcription modification of tested genes, and the cholesterol content maintain normal level during the whole exposure.
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Xiyan
authors.initials: X
authors.lastname: Mu
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chai
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Sen
authors.initials: S
authors.lastname: Pang
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China. Electronic address: Wangchengju@cau.edu.cn.
authors.forename: Chengju
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Sciences, China Agricultural University, Beijing, People's Republic of China. Electronic address: Lxf1966@263.net.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D004148:Dioxolanes / Q000633:toxicity*; D005260:Female; D005659:Fungicides, Industrial / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D008297:Male; D014230:Triazoles / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity; D015027:Zebrafish / Q000378:metabolism / Q000502:physiology*
chemical_list: D004148:Dioxolanes; D005659:Fungicides, Industrial; D014230:Triazoles; D014874:Water Pollutants, Chemical; D002784:Cholesterol; C115058:difenoconazole
keywords: Cholesterol; Difenoconazole; Sex differences; Zebrafish