title: Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle-stimulating hormone treatment or six intramuscular treatments over three days.
abstract: To reduce labor for superovulation treatment by twice-daily intramuscular (im) administration of FSH for more than 3 to 4 days, we investigated the superovulatory responses of Japanese Black cows to porcine FSH (pFSH) used as a single subcutaneous (sc) administration at two different doses in two different volumes of saline. In experiment 1, 20 Armour units (AU) of pFSH dissolved in either 10 mL (treatment A; n = 14) or 50 mL (treatment B; n = 14) of saline was administered subcutaneously in the neck region. In experiment 2, 30 AU of pFSH dissolved in either 10 mL (treatment C; n = 15) or 50 mL (treatment D; n = 15) of saline was administered subcutaneously in the neck region. The control animals in experiment 1 (n = 14) and experiment 2 (n = 15) received 20 AU of pFSH administered intramuscularly twice daily in decreasing doses for more than 3 days. In experiment 1, mean (±SEM) numbers of CL (15.4 ± 2.5, 18.1 ± 3.4, and 17.2 ± 2.6), total number of ova and embryos (12.9 ± 1.4, 15.9 ± 3.5, and 16.2 ± 2.8), and transferable embryos (7.5 ± 2.0, 10.4 ± 2.8, and 8.0 ± 2.1) did not differ among treatments A, B, and control. In experiment 2, mean (±SEM) numbers of CL (20.5 ± 4.3, 20.4 ± 2.7, and 20.1 ± 3.4), total number of ova and embryos (21.7 ± 4.2, 17.3 ± 3.4, and 16.5 ± 3.2), and transferable embryos (8.1 ± 1.6, 9.3 ± 2.2, and 9.5 ± 1.9) did not differ among treatments C, D, and control. Although there were no differences in serum pFSH concentrations among the three treatments at each of the time points in experiment 1, in experiment 2, the serum pFSH concentration at 6 and 8 hours after pFSH administration in treatment C (3.1 ± 0.8, 2.7 ± 0.5 ng/mL, mean ± SEM) was significantly greater (P < 0.05) than in the control (0.7 ± 0.1, 1.1 ± 0.2 ng/mL). At 10 hours after administration, the pFSH concentration had decreased and there were no differences among the three treatments at subsequent time points. These results suggest that increasing the volume of saline or the dose of pFSH does not affect the absorption pattern of pFSH administered as a single sc administration. In conclusions, single sc administration of pFSH at a dose of 20 or 30 AU dissolved in 10 or 50 mL of saline is able to induce a superovulatory response comparable with that obtained by twice-daily im administration in Japanese Black cows.
authors.affiliation: Aomori Prefectural Industrial Technology Research Center, Livestock Research Institute, Noheji, Aomori, Japan. Electronic address: Shingo_hiraizumi@aomori-itc.or.jp.
authors.forename: S
authors.initials: S
authors.lastname: Hiraizumi
authors.affiliation: Livestock Experiment Station, Akita Prefectural Agriculture Forestry and Fisheries Research Center, Akita, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nishinomiya
authors.affiliation: Miyagi Prefectural Livestock Experiment Station, Miyagi, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oikawa
authors.affiliation: Kanagawa Prefectural Livestock Technology Center, Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sakagami
authors.affiliation: Shizuoka Prefectural Research Institute of Animal Industry, Shizuoka, Japan.
authors.forename: F
authors.initials: F
authors.lastname: Sano
authors.affiliation: Nara prefectural Livestock Technology Center, Nara, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Nishino
authors.affiliation: Oita Prefectural Agriculture, Forestry and Fisheries Research Center Livestock Research Institute, Oita, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kurahara
authors.affiliation: Aomori Prefectural Industrial Technology Research Center, Livestock Research Institute, Noheji, Aomori, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Nishimoto
authors.affiliation: Aomori Prefectural Industrial Technology Research Center, Livestock Research Institute, Noheji, Aomori, Japan.
authors.forename: O
authors.initials: O
authors.lastname: Ishiyama
authors.affiliation: Laboratory of Experimental Animal Science, Kitasato University, Towada Aomori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: National Livestock Breeding Center, Fukushima, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hashiyada
mesh_terms: D000818:Animals; D002417:Cattle / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D007273:Injections, Intramuscular; D007279:Injections, Subcutaneous; D013480:Superovulation / Q000187:drug effects*
chemical_list: D005640:Follicle Stimulating Hormone
keywords: Japanese Black cow; Single subcutaneous administration; Superovulatory response