title: Phytochemical composition, antioxidant activity and HPLC profiles of Swertia species from Western Ghats.
abstract: Swertia chirayita is one of the potential medicinal plants of the family Gentianaceae in traditional medicine. Due to its high demand and scarcity, trade of chirayita is affected by adulterants. Swertia species from Western Ghats were compared with S. chirayita for phytochemical characterisation and antioxidant activities by using different extracts. This study revealed that acetone is the best extraction solvent of phenolic and flavonoid compounds with antioxidant properties as compared with other extracts. S. chirayita showed better antioxidant activity than other species with highest content of phenolics and flavonoids. Among the species from Western Ghats, Swertia minor has better antioxidant properties with higher content of phenolics and flavonoids when compared with S. chirayita. Gallic acid was detected in all species under study by using HPLC analysis. The Swertia species under study showed similar phytochemical properties and antioxidant potential and hence their use as substitute to S. chirayita needs to be further investigated.
authors.affiliation: a Laboratory of Cytogenetics and Plant Breeding, Department of Botany , Shivaji University , Kolhapur 416004 , India.
authors.forename: Parthraj
authors.initials: P
authors.lastname: Kshirsagar
authors.forename: Jaykumar
authors.initials: J
authors.lastname: Chavan
authors.forename: Mansingraj
authors.initials: M
authors.lastname: Nimbalkar
authors.forename: Shrirang
authors.initials: S
authors.lastname: Yadav
authors.forename: Ghansham
authors.initials: G
authors.lastname: Dixit
authors.forename: Nikhil
authors.initials: N
authors.lastname: Gaikwad
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000737:chemistry; D010636:Phenols / Q000737:chemistry; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D029967:Swertia / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts
keywords: HPLC; Swertia; antioxidant activity; flavonoids; phenolic compounds