title: Rapid geographical differentiation of the European spread brown macroalga Sargassum muticum using HRMAS NMR and Fourier-Transform Infrared spectroscopy.
abstract: Two recent techniques based on chemical footprinting analysis, HRMAS NMR and FTIR spectroscopy, were tested on a brown macroalgal model. These powerful and easily-to-use techniques allowed us to discriminate Sargassum muticum specimens collected in five different countries along Atlantic coasts, from Portugal to Norway. HRMAS NMR and FTIR permitted the obtaining of an overview of metabolites produced by the alga. Based on spectra analysis, results allowed us to successfully group the samples according to their geographical origin. HRMAS NMR and FTIR spectroscopy respectively point out the relation between the geographical localization and the chemical composition and demonstrated macromolecules variations regarding to environmental stress. Then, our results are discussed in regard of the powerful of these techniques together with the variability of the main molecules produced by Sargassum muticum along the Atlantic coasts.
authors.affiliation: LEMAR UMR CNRS UBO IRD IFREMER 6539, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d׳Urville, Plouzané 29280, France.
authors.forename: Anaëlle
authors.initials: A
authors.lastname: Tanniou
authors.affiliation: GEPEA UMR CNRS 6144, Université de Nantes, Laboratoire GEPEA, CRTT, 44602 Saint-Nazaire, France; LBCM EA 3884, Université de Bretagne Sud (UBS), IUEM, Campus de Tohannic, 56000 Vannes, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Vandanjon
authors.affiliation: GEPEA UMR CNRS 6144, Université de Nantes, Laboratoire GEPEA, CRTT, 44602 Saint-Nazaire, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gonçalves
authors.affiliation: Service RMN-RPE, UFR Sciences et Techniques, Université de Bretagne Occidentale (UBO), Avenue Le Gorgeu, 29200 Brest, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.affiliation: LEMAR UMR CNRS UBO IRD IFREMER 6539, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d׳Urville, Plouzané 29280, France. Electronic address: valerie.stiger@univ-brest.fr.
authors.forename: Valérie
authors.initials: V
authors.lastname: Stiger-Pouvreau
mesh_terms: D001267:Atlantic Ocean; D005060:Europe; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D010802:Phylogeny; D011157:Population Dynamics; D025341:Principal Component Analysis; D044647:Sargassum / Q000737:chemistry* / Q000145:classification* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
keywords: FTIR spectroscopy; Geographical origin discrimination; HRMAS NMR; Latitudinal gradient; Sargassum muticum