title: Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application.
abstract: OBJECTIVE
  A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.
  
  
  METHODS
  Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.
  
  
  RESULTS
  A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.
  
  
  CONCLUSIONS
  The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Tao
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China. Electronic address: lin.jun@sipi.com.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China.
authors.forename: You-Jia
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai 200040, China. Electronic address: baoquanzhu@sipi.com.cn.
authors.forename: Bao-Quan
authors.initials: BQ
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D011971:Receptors, Immunologic / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011994:Recombinant Proteins
chemical_list: D000911:Antibodies, Monoclonal; C431503:LAIR-2 receptor; D011971:Receptors, Immunologic; D011994:Recombinant Proteins
keywords: ELISA kit; Human Secreted CD306/LAIR-2; Monoclonal antibody; Polyclonal antibody