title: Whole-cell Gluconobacter oxydans biosensor for 2-phenylethanol biooxidation monitoring.
abstract: A microbial biosensor for 2-phenylethanol (2-PE) based on the bacteria Gluconobacter oxydans was developed and applied in monitoring of a biotechnological process. The cells of G. oxydans were immobilized within a disposable polyelectrolyte complex gel membrane consisting of sodium alginate, cellulose sulphate and poly(methylene-co-guanidine) attached onto a miniaturized Clark oxygen electrode, forming whole cell amperometric biosensor. Measured changes in oxygen concentration were proportional to changes in 2-PE concentration. The biosensor sensitivity was 864 nA mM(-1) (RSD=6%), a detection limit of 1 μM, and the biosensor response towards 2-PE was linear in the range 0.02-0.70 mM. The biosensor preserved 93% of its initial sensitivity after 7h of continuous operation and exhibited excellent storage stability with loss of only 6% of initial sensitivity within two months, when stored at 4°C. The developed system was designed and successfully used for an off-line monitoring of whole course of 2-PE biooxidation process producing phenylacetic acid (PA) as industrially valuable aromatic compound. The biosensor measurement did not require the use of hazardous organic solvent. The biosensor response to 2-PE was not affected by interferences from PA and phenylacetaldehyde at concentrations present in real samples during the biotransformation and the results were in a very good agreement with those obtained via gas chromatography.
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schenkmayerová
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Anikó
authors.initials: A
authors.lastname: Bertóková
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Jana
authors.initials: J
authors.lastname: Sefčovičová
authors.affiliation: Axxence Slovakia s.r.o., Poštová 1, SK-81106 Bratislava, Slovakia.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Stefuca
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Marek
authors.initials: M
authors.lastname: Bučko
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Alica
authors.initials: A
authors.lastname: Vikartovská
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Peter
authors.initials: P
authors.lastname: Gemeiner
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
authors.forename: Ján
authors.initials: J
authors.lastname: Tkáč
authors.affiliation: Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia. Electronic address: jaroslav.katrlik@savba.sk.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Katrlík
mesh_terms: D015374:Biosensing Techniques; D020600:Gluconobacter oxydans / Q000378:metabolism*; D010084:Oxidation-Reduction; D010626:Phenylethyl Alcohol / Q000378:metabolism*
chemical_list: D010626:Phenylethyl Alcohol
keywords: 2-Phenylethanol; Biosensor; Electrochemistry; Gluconobacter oxydans; Immobilization; Phenylacetic acid