title: Preparation, characterization and application of N-methylene phosphonic acid chitosan grafted magnesia-zirconia stationary phase.
abstract: A hydrophilic stationary phase (SP) was prepared through grafting N-methylene phosphonic acid chitosan on magnesia-zirconia particles (P-CTS-MgO-ZrO2) via Lewis acid-base interaction. The resulting material was characterized by thermogravimetric analysis, Fourier transform infrared spectroscopy, scanning electron microscope and nitrogen adsorption analysis. The chromatographic performance of P-CTS-MgO-ZrO2 was systemically evaluated by studying effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the novel SP provided hydrophilic, electrostatic-repulsion and ion-exchange interactions. Compared to the bare MgO-ZrO2, P-CTS-MgO-ZrO2 exhibited superior peak shape, reasonable resolution and reduced analysis time in separation of basic analytes. Besides, remarkable resolving power of acids, i.e. six non-steroidal anti-inflammatory drugs which failed to be eluted from the bare MgO-ZrO2, was obtained with the theoretical plate number (N/m) of 4653-31313, asymmetry factor <1.21 and the resolution of 1.6-3.4. Finally, P-CTS-MgO-ZrO2 SP was applied to separate monosaccharides, phospholipids and peptides. P-CTS-MgO-ZrO2 was a promising hydrophilic SP for wide applications.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Huang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address: shizg@whu.edu.cn.
authors.forename: Zhi-guo
authors.initials: ZG
authors.lastname: Shi
mesh_terms: D048271:Chitosan / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D010757:Phosphorous Acids / Q000737:chemistry*; D015040:Zirconium / Q000378:metabolism*
chemical_list: D010757:Phosphorous Acids; D048271:Chitosan; D015040:Zirconium; D008274:Magnesium; C028541:zirconium oxide
keywords: Chitosan; Hydrophilic interaction chromatography; Magnesia-zirconia