title: Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility.
abstract: In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds. Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%. Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity. Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds. A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.
authors.affiliation: Department of Applied Mechanics (Biomedical Engineering), Motilal Nehru National Institute of Technology, Allahabad, India pkd_437@yahoo.com.
authors.forename: Abijeet Singh
authors.initials: AS
authors.lastname: Mehta
authors.affiliation: Department of Chemistry, Motilal Nehru National Institute of Technology, Allahabad, India.
authors.forename: Brijesh K
authors.initials: BK
authors.lastname: Singh
authors.affiliation: School of Cellular & Molecular Medicine, University of Bristol, Bristol, UK.
authors.forename: Nandita
authors.initials: N
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Motilal Nehru National Institute of Technology, Allahabad, India.
authors.forename: D
authors.initials: D
authors.lastname: Archana
authors.affiliation: Department of Applied Mechanics (Biomedical Engineering), Motilal Nehru National Institute of Technology, Allahabad, India.
authors.forename: Kirti
authors.initials: K
authors.lastname: Snigdha
authors.affiliation: School of Chemistry, University of Bristol, Bristol, UK.
authors.forename: Robert
authors.initials: R
authors.lastname: Harniman
authors.affiliation: Advanced Composites Centre for Innovation and Science (ACCIS), Aerospace Engineering, University of Bristol, Bristol, UK.
authors.forename: Sameer S
authors.initials: SS
authors.lastname: Rahatekar
authors.affiliation: Department of Applied Mechanics (Biomedical Engineering), Motilal Nehru National Institute of Technology, Allahabad, India.
authors.forename: R P
authors.initials: RP
authors.lastname: Tewari
authors.affiliation: Department of Chemistry, Motilal Nehru National Institute of Technology, Allahabad, India.
authors.forename: P K
authors.initials: PK
authors.lastname: Dutta
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry*; D012831:Bombyx; D048271:Chitosan / Q000737:chemistry; D019245:Compressive Strength; D016503:Drug Delivery Systems; D005839:Gentamicins / Q000008:administration & dosage*; D020723:Glucuronic Acid / Q000737:chemistry; D006461:Hemolysis; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D047011:Silk / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D005839:Gentamicins; D006603:Hexuronic Acids; D047011:Silk; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Silk fibroin; chitosan; drug delivery; gelatin; gentamicin; scaffolds