title: Characterization of a purified decolorizing detergent-stable peroxidase from Streptomyces griseosporeus SN9.
abstract: A novel extracellular lignin peroxidase (called LiP-SN) was produced and purified from a newly isolated Streptomyces griseosporeus strain SN9. The findings revealed that the pure enzyme was a monomeric protein with an estimated molecular mass of 43 kDa and a Reinheitzahl value of 1.63. The 19 N-terminal residue sequence of LiP-SN showed high homology with those of Streptomyces peroxidases. Its optimum pH and temperature were pH 8.5 and 65 °C, respectively. The enzyme was inhibited by sodium azide and potassium cyanide, suggesting the presence of heme components in its tertiary structure. Its catalytic efficiency was higher than that of the peroxidase from Streptomyces albidoflavus strain TN644. Interestingly, LiP-SN showed marked dye-decolorization efficiency and stability toward denaturing, oxidizing, and bleaching agents, and compatibility with EcoVax and Dipex as laundry detergents for 48 h at 40 °C. These properties make LiP-SN a potential candidate for future applications in distaining synthetic dyes and detergent formulations.
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
authors.forename: Hatem
authors.initials: H
authors.lastname: Rekik
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
authors.forename: Zaraî Jaouadi
authors.initials: ZJ
authors.lastname: Nadia
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
authors.forename: Wacim
authors.initials: W
authors.lastname: Bejar
authors.affiliation: National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria.
authors.forename: Sidali
authors.initials: S
authors.lastname: Kourdali
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
authors.forename: Mouna
authors.initials: M
authors.lastname: Belhoul
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia.
authors.forename: Maher
authors.initials: M
authors.lastname: Hmidi
authors.affiliation: National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPCB), University of Blida, 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria.
authors.forename: Amina
authors.initials: A
authors.lastname: Benkiar
authors.affiliation: National Centre for Research and Development of Fisheries and Aquaculture (CNRDPA), 11, Bd Amirouche PO Box 67, Bou Ismaïl 42415, Tipaza, Algeria; Laboratory of Natural Products Chemistry and Biomolecules (LNPCB), University of Blida, 1, Road of Soumaâ, PO Box 270, 09000 Blida, Algeria.
authors.forename: Abdelmalek
authors.initials: A
authors.lastname: Badis
authors.affiliation: Klin Productions, Detergents and Maintenance Products Industry, Road of Hencha Km 1.5, Z.I. Jbeniana, PO Box 247, Sfax 3080, Tunisia.
authors.forename: Naim
authors.initials: N
authors.lastname: Sallem
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia. Electronic address: samir.bejar@cbs.rnrt.tn.
authors.forename: Samir
authors.initials: S
authors.lastname: Bejar
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, PO Box 1177, Sfax 3018, Tunisia. Electronic address: bassem.jaouadi@yahoo.fr.
authors.forename: Bassem
authors.initials: B
authors.lastname: Jaouadi
mesh_terms: D000595:Amino Acid Sequence; D003902:Detergents / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry; D007700:Kinetics; D008670:Metals / Q000737:chemistry; D008970:Molecular Weight; D009195:Peroxidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature
chemical_list: D003902:Detergents; D007477:Ions; D008670:Metals; D012336:RNA, Ribosomal, 16S; D006861:Hydrogen Peroxide; D009195:Peroxidase
keywords: Detergent; Lignin peroxidase; Streptomyces