title: Chemiluminescence lateral flow immunoassay based on Pt nanoparticle with peroxidase activity.
abstract: A lateral flow immunoassay (LF-immunoassay) with an enhanced sensitivity and thermostability was developed by using Pt nanoparticles with a peroxidase activity. The Pt nanoparticles were synthesized by citrate reduction method, and the peroxidase activity of Pt nanoparticles was optimized by adjusting reaction conditions. The peroxidase activity was estimated by using Michaelis-Menten kinetics model with TMB as a chromogenic substrate. The kinetics parameters of KM and Vmax were calculated and compared with horseradish peroxidase (HRP). The thermal stability of the Pt nanoparticles was compared with horseradish peroxidase (HRP) according to the storage temperature and long-term storage period. The feasibility of lateral flow immunoassay with a chemiluminescent signal band was demonstrated by the detection of human chorionic gonadotropin (hCG) as a model analyte, and the sensitivity was determined to be improved by as much as 1000-fold compared to the conventional rapid test based on colored gold-colloids.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, South Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, South Korea.
authors.forename: Ha-Wook
authors.initials: HW
authors.lastname: Jung
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, South Korea.
authors.forename: Young Wook
authors.initials: YW
authors.lastname: Chang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, South Korea.
authors.forename: Hyung-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Korea Institute of Science and Technology (KIST), Seoul, South Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 120-742, South Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry; D006063:Chorionic Gonadotropin / Q000032:analysis; D002951:Citrates / Q000737:chemistry; D005240:Feasibility Studies; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay / Q000379:methods*; D007700:Kinetics; D008163:Luminescent Measurements; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D013696:Temperature
chemical_list: D006063:Chorionic Gonadotropin; D002951:Citrates; D008567:Membranes, Artificial; D010984:Platinum; D002482:Cellulose; D006735:Horseradish Peroxidase
keywords: Chemiluminescence; Lateral flow immunoassay; Peroxidase; Pt nanoparticle