title: Biodistribution of PLGA and PLGA/chitosan nanoparticles after repeat-dose oral delivery in F344 rats for 7 days.
abstract: OBJECTIVE
  To quantify in vivo the biodistribution of poly(lactic-co-glycolic) acid (PLGA) and PLGA/chitosan nanoparticles (PLGA/Chi NPs) and assess if the positive charge of chitosan significantly enhances nanoparticle absorption in the GI tract.
  
  
  METHODS
  PLGA and PLGA/Chi NPs covalently linked to tetramethylrhodamine-5-isothiocyanate (TRITC) were orally administered to F344 rats for 7 days, and the biodistribution of fluorescent NPs was analyzed in different organs.
  
  
  RESULTS
  The highest amount of particles (% total dose/g) was detected for both treatments in the spleen, followed by intestine and kidney, and then by liver, lung, heart and brain, with no significant difference between PLGA and PLGA/Chi NPs.
  
  
  CONCLUSIONS
  Only a small percentage of orally delivered NPs was detected in the analyzed organs. The positive charge conferred by chitosan was not sufficient to improve the absorption of the PLGA/Chi NPs over that of PLGA NPs.
authors.affiliation: Biological & Agricultural Engineering Department, LSU & LSU AgCenter, Baton Rouge, LA, USA.
authors.forename: Sara M
authors.initials: SM
authors.lastname: Navarro
authors.forename: Caleb
authors.initials: C
authors.lastname: Darensbourg
authors.forename: Linda
authors.initials: L
authors.lastname: Cross
authors.forename: Rhett
authors.initials: R
authors.lastname: Stout
authors.forename: Diana
authors.initials: D
authors.lastname: Coulon
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Astete
authors.forename: Timothy
authors.initials: T
authors.lastname: Morgan
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Sabliov
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002626:Chemistry, Pharmaceutical; D048271:Chitosan / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D004334:Drug Administration Schedule; D004337:Drug Carriers; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000493:pharmacokinetics; D007408:Intestinal Absorption; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D036103:Nanotechnology; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011916:Rats, Inbred F344; D012235:Rhodamines / Q000008:administration & dosage / Q000493:pharmacokinetics; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D014018:Tissue Distribution
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D012235:Rhodamines; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C009434:tetramethylrhodamine isothiocyanate; D048271:Chitosan