title: Advanced LSI-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes.
abstract: We have developed a large-scale integrated (LSI) complementary metal-oxide semiconductor (CMOS)-based amperometric sensor array system called "Bio-LSI" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. In this study, we newly developed a Bio-LSI chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of Bio-LSI. The light shield created by the top metal layer of the LSI chip significantly reduces the noise generated by the photocurrent, whose value is less than 1% of the previous Bio-LSI without the light shield. The mode-selectable function enables the individual operation of 400 electrodes in off, electrometer, V1, and V2 mode. The off-mode cuts the electrode from the electric circuit. The electrometer-mode reads out the electrode potential. The V1-mode and the V2-mode set the selected sensor electrode at two different independent voltages and read out the current. We demonstrated the usefulness of the mode-selectable function. First, we displayed a dot picture based on the redox reactions of 2.0 mM ferrocenemethanol at 400 electrodes by applying two different independent voltages using the V1 and V2 modes. Second, we carried out a simultaneous detection of O2 and H2O2 using the V1 and V2 modes. Third, we used the off and V1 modes for the modification of the osmium-polyvinylpyridine gel polymer containing horseradish peroxidase (Os-HRP) at the selected electrodes, which act as sensors for H2O2. These results confirm that the advanced version of Bio-LSI is a promising tool that can be applied to a wide range of analytical fields.
authors.affiliation: Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aramaki-Aza-Aoba, Aoba-ku, Sendai 980-8579, Japan. inoue@bioinfo.che.tohoku.ac.jp.
authors.forename: Kumi Y
authors.initials: KY
authors.lastname: Inoue
authors.forename: Masahki
authors.initials: M
authors.lastname: Matsudaira
authors.forename: Masanori
authors.initials: M
authors.lastname: Nakano
authors.forename: Kosuke
authors.initials: K
authors.lastname: Ino
authors.forename: Chika
authors.initials: C
authors.lastname: Sakamoto
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kanno
authors.forename: Reyushi
authors.initials: R
authors.lastname: Kubo
authors.forename: Ryota
authors.initials: R
authors.lastname: Kunikata
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kira
authors.forename: Atsushi
authors.initials: A
authors.lastname: Suda
authors.forename: Ryota
authors.initials: R
authors.lastname: Tsurumi
authors.forename: Toshihito
authors.initials: T
authors.lastname: Shioya
authors.forename: Shinya
authors.initials: S
authors.lastname: Yoshida
authors.forename: Masanori
authors.initials: M
authors.lastname: Muroyama
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ishikawa
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Shiku
authors.forename: Shiro
authors.initials: S
authors.lastname: Satoh
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Esashi
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Matsue
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D008027:Light; D055668:Photochemical Processes; D012666:Semiconductors