title: Nasal IgA secretion in a murine model of acute stress. The possible role of catecholamines.
abstract: Stress stimuli affect the immune system of the mucosa, and in particular IgA secretion. It is well documented that intense psychological and physical stress can increase susceptibility to infection by diverse pathogens in the upper respiratory tract. Our workgroup reported that chronic stress caused by immobilization elicits a decrease in nasal IgA levels in mice. Here, we explore how acute stress (caused by 4h of immobilization) affects IgA secretion in the nasal mucosa, and the possible role of the sympathetic nervous system in this effect. Nine-week-old male CD1 mice were divided into four groups: control, chemical sympathectomy (with 6-OHDA) and treatment with nadolol (5mg/kg) or phentolamine (15mg/kg). All these groups were subdivided into stressed and unstressed animals. The parameters evaluated included plasma corticosterone and epinephrine (only in control groups), SIgA levels (by ELISA) and SIgA expression (by Western Blot) in nasal fluid, percentage of IgA+ plasma cells, and mRNA expression of heavy alpha chain, pIgR, TNFα and TGFβ in nasal mucosa. Acute stress reduced the percentage of IgA+ cells while increasing the levels of IgA, the two hormones, and the mRNA expression of heavy alpha chain, pIgR, TNFα and TGFβ, which resulted in greater synthesis and transport of IgA. The treatments with 6-OHDA and α- and β-adrenergic receptor blockers suggest that sympathetic innervation by both types of adrenergic receptors is important for the control of SIgA secretion in nasal mucosa during acute stress. The increase in this parameter depended on the cytokines involved in IgA synthesis and transport.
authors.affiliation: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340 México, D.F., México. Electronic address: rosana1112@yahoo.com.mx.
authors.forename: Rosa Adriana
authors.initials: RA
authors.lastname: Jarillo-Luna
authors.affiliation: Departamento de Microbiología, UBIPRO, FES-Iztacala, UNAM, Avenida de los Barrios s/n, 54090 Tlalnepantla, Estado de México, México.
authors.forename: Victor
authors.initials: V
authors.lastname: Rivera-Aguilar
authors.affiliation: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340 México, D.F., México.
authors.forename: Judith
authors.initials: J
authors.lastname: Pacheco-Yépez
authors.affiliation: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340 México, D.F., México.
authors.forename: Marycarmen
authors.initials: M
authors.lastname: Godínez-Victoria
authors.affiliation: Laboratorio de Investigación en Nutrición, Facultad de Medicina, Universidad Autónoma del Estado de México, Paseo Tollocan, Esquina Jesús Carranza, Col. Moderna de la Cruz, Toluca, Estado de México CP 50180, México.
authors.forename: Rigoberto
authors.initials: R
authors.lastname: Oros-Pantoja
authors.affiliation: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340 México, D.F., México.
authors.forename: Angel
authors.initials: A
authors.lastname: Miliar-García
authors.affiliation: Sección de Estudios de Postgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luís y Díaz Mirón, CP 11340 México, D.F., México.
authors.forename: Rafael
authors.initials: R
authors.lastname: Campos-Rodríguez
mesh_terms: D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D003345:Corticosterone; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007070:Immunoglobulin A / Q000378:metabolism*; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D008297:Male; D051379:Mice; D009092:Mucous Membrane / Q000187:drug effects / Q000378:metabolism*; D009248:Nadolol / Q000494:pharmacology; D016627:Oxidopamine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013315:Stress, Psychological / Q000378:metabolism*; D013563:Sympathectomy, Chemical; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D002395:Catecholamines; D016207:Cytokines; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D009248:Nadolol; D016627:Oxidopamine; D003345:Corticosterone; D010646:Phentolamine
keywords: Acute stress; Catecholamines; Cytokines; IgA; pIgR