title: Separation properties of saccharides on a hydrophilic stationary phase having hydration layer formed zwitterionic copolymer.
abstract: A novel water-holding adsorbent bonded with a zwitterionic polymer, diallylamine-maleic acid copolymer, was developed. With this adsorbent, hydrophilic solutes are partitioned by a hydration layer that forms on the zwitterions, as a main separating force. When the adsorbent was used to separate saccharides by normal-phase partition chromatography, the saccharides eluted in the order, mono-, di- and trisaccharide. The elution profile for mono- and di-saccharides was similar but not identical to that on anion exchange columns. This indicated that the adsorbent exhibited a complex retention behavior by the existence of both anion and cation exchange moieties in the functional polymer. Selecting Na(+) as a counter-ion of the maleate moiety enhanced the retention of saccharide. When used in an high performance liquid chromatography (HPLC) system with gradient elution, the adsorbent enabled the simultaneous analysis of mono-, di- and oligosaccharides.
authors.affiliation: Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi-shi, Tokyo 206-8577, Japan. Electronic address: w-kamichatani@filcon.co.jp.
authors.forename: Waka
authors.initials: W
authors.lastname: Kamichatani
authors.affiliation: Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi-shi, Tokyo 206-8577, Japan; College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai-shi, Aichi 487-8501, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamamoto
mesh_terms: D000499:Allylamine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004186:Disaccharidases / Q000302:isolation & purification*; D057927:Hydrophobic and Hydrophilic Interactions; D008298:Maleates / Q000737:chemistry; D009005:Monosaccharides / Q000302:isolation & purification*; D009844:Oligosaccharides / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D008298:Maleates; D009005:Monosaccharides; D009844:Oligosaccharides; D011108:Polymers; D014867:Water; D000499:Allylamine; C030272:maleic acid; D004186:Disaccharidases
keywords: Hydration layer; Hydrophilic stationary phase; Normal-phase partition chromatography; Saccharide; Water-holding adsorbent; Zwitterionic copolymer