title: A carboxylated graphene and aptamer nanocomposite-based aptasensor for sensitive and specific detection of hemin.
abstract: A unique nanocomposite was crafted by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR). Infrared spectroscopy, Raman spectroscopy and diffuse reflectance spectra suggested that -NHCO- covalent bonds were formed between HBA and COO-GR. The resulting COO-GR/HBA functionalized electrode was used as a novel label-free biosensor. The square wave voltammetry was employed to realize the selective and specific detection of hemin. The obtained aptasensor possessed excellent performance with a detection limit of 0.64 nmol L(-1) (S/N=3) and a linear range from 1 to 150 nmol L(-1). Moreover, COO-GR was shown to be a promising candidate in making aptasensors, carrying advantages over graphene in terms of the simplicity of sensor preparation and the reduction of background noise.
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China. Electronic address: yhxiao@njnu.edu.cn.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097, China. Electronic address: yangxiaodi@njnu.edu.cn.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Yang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006427:Hemin / Q000032:analysis*; D057230:Limit of Detection; D053761:Nanocomposites / Q000737:chemistry*; D012996:Solutions; D014867:Water
chemical_list: D052157:Aptamers, Nucleotide; D012996:Solutions; D014867:Water; D006427:Hemin; D006108:Graphite
keywords: Carboxylated grapheme; Functionalized electrode; Nanocomposite aptasensor