title: Neural mobilization promotes nerve regeneration by nerve growth factor and myelin protein zero increased after sciatic nerve injury.
abstract: Neurotrophins are crucial in relation to axonal regrowth and remyelination following injury; and neural mobilization (NM) is a noninvasive therapy that clinically is effective in neuropathic pain treatment, but its mechanisms remains unclear. We examined the effects of NM on the regeneration of sciatic nerve after chronic constriction injury (CCI) in rats. The CCI was performed on adult male rats, submitted to 10 sessions of NM, starting 14 days after CCI. Then, the nerves were analyzed using transmission electron microscopy and western blot for neural growth factor (NGF) and myelin protein zero (MPZ). We observed an increase of NGF and MPZ after CCI and NM. Electron microscopy revealed that CCI-NM samples had high numbers of axons possessing myelin sheaths of normal thickness and less inter-axonal fibrosis than the CCI. These data suggest that NM is effective in facilitating nerve regeneration and NGF and MPZ are involved in this effect.
authors.affiliation: Department of Anatomy, Laboratory of Functional Neuroanatomy of Pain, Institute of Biomedical Sciences, University of São Paulo , São Paulo , Brazil .
authors.forename: Joyce Teixeira
authors.initials: JT
authors.lastname: da Silva
authors.forename: Fabio Martinez Dos
authors.initials: FM
authors.lastname: Santos
authors.forename: Aline Caroline
authors.initials: AC
authors.lastname: Giardini
authors.forename: Daniel de Oliveira
authors.initials: Dde O
authors.lastname: Martins
authors.forename: Mara Evany
authors.initials: ME
authors.lastname: de Oliveira
authors.forename: Adriano Polican
authors.initials: AP
authors.lastname: Ciena
authors.forename: Vanessa Pacciari
authors.initials: VP
authors.lastname: Gutierrez
authors.forename: Ii-Sei
authors.initials: IS
authors.lastname: Watanabe
authors.forename: Luiz Roberto G de
authors.initials: LR
authors.lastname: Britto
authors.forename: Marucia
authors.initials: M
authors.lastname: Chacur
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D026201:Musculoskeletal Manipulations; D018993:Myelin P0 Protein / Q000235:genetics / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism*; D009416:Nerve Regeneration; D059348:Peripheral Nerve Injuries / Q000378:metabolism* / Q000628:therapy; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000502:physiology
chemical_list: D018993:Myelin P0 Protein; D020932:Nerve Growth Factor
keywords: MPZ; NGF; nerve regeneration; neural mobilization; sciatic nerve