title: Dye-free determination of the focalization position for the hollow fiber flow field flow fractionation (HF5) of proteins.
abstract: Proteins are separated in field flow fractionation (FFF) according to a well-established mechanism described as the "Normal or Brownian" mode. In the case of the sub-technique using a hollow fiber, the focalization/relaxation position can be observed visually only with a transparent holder and using dyes as samples. Whatever the choice of instrumentation, a dye-free method is proposed to determine the center of the zone from experimental fractograms by means of only two sample elutions. It is also possible to determine and model the kinematics of the sample toward the equilibrium focalization/relaxation position as well as the real dimensions of the fiber during the separation process.
authors.affiliation: Chemistry Department, ALBA'ATH University, Homs, Syria.
authors.forename: Tayssir
authors.initials: T
authors.lastname: Ibrahim
authors.forename: Daniel
authors.initials: D
authors.lastname: Daugeron
authors.forename: Serge
authors.initials: S
authors.lastname: Battu
authors.forename: Issa-Ali
authors.initials: IA
authors.lastname: Moustafa
authors.forename: Claire
authors.initials: C
authors.lastname: Lefort
authors.forename: Philippe
authors.initials: P
authors.lastname: Cardot
mesh_terms: D000465:Algorithms; D004396:Coloring Agents / Q000302:isolation & purification; D031521:Fractionation, Field Flow / Q000379:methods*; D007700:Kinetics; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D004396:Coloring Agents; D011506:Proteins