title: Identification of transcription factors linked to cell cycle regulation in Arabidopsis.
abstract: Cell cycle is an essential process in growth and development of living organisms consists of the replication and mitotic phases separated by 2 gap phases; G1 and G2. It is tightly controlled at the molecular level and especially at the level of transcription. Precise regulation of the cell cycle is of central significance for plant growth and development and transcription factors are global regulators of gene expression playing essential roles in cell cycle regulation. This study has uncovered TFs that are involved in the control of cell cycle progression. With the aid of multi-parallel quantitative RT-PCR, the expression changes of 1880 TFs represented in the Arabidopsis TF platform was monitored in Arabidopsis synchronous MM2d cells during a 19 h period representing different time points corresponding to the 4 cell cycle phases after treatment of MM2d cells with Aphidicolin. Comparative TF expression analyses performed on synchronous cells resulted in the identification of 239 TFs differentially expressed during the cell cycle, while about one third of TFs were constitutively expressed through all time points. Phase-specific TFs were also identified.
authors.affiliation: a Max-Planck Institute of Molecular Plant Physiology ; Am Mühlenberg 1; Potsdam-Golm , Germany.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Dehghan Nayeri
mesh_terms: D016590:Aphidicolin / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016000:Cluster Analysis; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D013535:Suspensions; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D013535:Suspensions; D014157:Transcription Factors; D016590:Aphidicolin
keywords: Arabidopsis; QRT-PCR; aphidicolin; cell cycle; suspension culture; synchronization; transcription factors