title: Poly(L-lactic acid) membranes: absence of genotoxic hazard and potential for drug delivery.
abstract: The use of poly(L-lactic acid) (PLA) has been considered an important alternative for medical devices once this polyester presents biomechanical, optical and biodegradable properties. Moreover, the use of PLA results in less inflammatory reactions and more recently it has been proposed its application in drug delivery systems. Genotoxicological evaluations are considered part of the battery assays in toxicological analysis. Considering the wide applications of PLA, the present work evaluated the potential cytotoxic and genotoxic effects of PLA in CHO-K1 cells, as well as its physicochemical properties. No cytotoxic effects of PLA were detected by colorimetric tetrazolium assay (XTT) analysis, and the clonogenic survival assay showed that PLA did not disrupt the replicative cell homeostasis, neither exhibited genotoxic effects as evidenced by comet and micronucleus assays. Thermogravimetric properties of PLA were not altered after contact with cells and this film exhibited ability in absorb and release Europium(III) complex. All these data suggest genotoxicological safety of PLA for further applications in drug delivery systems.
authors.affiliation: Department of Surgery, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Nelson
authors.initials: N
authors.lastname: Uzun
authors.affiliation: Laboratory of Genetics and Molecular Biology, University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Franca, SP CEP 14404-600, Brazil.
authors.forename: Thomás Duzzi
authors.initials: TD
authors.lastname: Martins
authors.affiliation: Laboratory of Genetics and Molecular Biology, University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Franca, SP CEP 14404-600, Brazil.
authors.forename: Gabriella Machado
authors.initials: GM
authors.lastname: Teixeira
authors.affiliation: Laboratory of Genetics and Molecular Biology, University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Franca, SP CEP 14404-600, Brazil.
authors.forename: Nayanne Larissa
authors.initials: NL
authors.lastname: Cunha
authors.affiliation: Department of Surgery, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Rogério Belle
authors.initials: RB
authors.lastname: Oliveira
authors.affiliation: Laboratory of Genetics and Molecular Biology, University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Franca, SP CEP 14404-600, Brazil.
authors.forename: Eduardo José
authors.initials: EJ
authors.lastname: Nassar
authors.affiliation: Laboratory of Genetics and Molecular Biology, University of Franca, Av. Dr. Armando Salles de Oliveira, 201, Franca, SP CEP 14404-600, Brazil. Electronic address: rasantosgen@yahoo.com.br.
authors.forename: Raquel Alves
authors.initials: RA
authors.lastname: Dos Santos
mesh_terms: D000818:Animals; D016466:CHO Cells; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D016503:Drug Delivery Systems; D019344:Lactic Acid / Q000737:chemistry* / Q000633:toxicity*; D008567:Membranes, Artificial; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity*
chemical_list: D008567:Membranes, Artificial; D009153:Mutagens; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)
keywords: Comet assay; Drug delivery; Genotoxicity; Micronucleus assay; Poly(l-lactic acid)