title: Aldosterone, corticosterone, and thyroid hormone and their influence on respiratory control development in Lithobates catesbeianus: An in vitro study.
abstract: The emergence of air breathing during Lithobates catesbeianus development requires significant changes to the brainstem circuits that generate and regulate breathing; however, the mechanisms responsible for initiating this transformation remain largely unknown. Because amphibian metamorphosis is regulated by hormones such as aldosterone, corticosterone, and thyroid hormone (T3), we tested the hypothesis that exposing the brainstem to these hormones augments the fictive air breathing frequency in pre-metamorphic tadpoles. Brainstems were isolated and were placed either in the recording chamber (acute; 1h+1h recovery) or in a bottle (chronic exposure; 24h) for treatment. Brainstems were exposed to artificial cerebrospinal fluid (aCSF; sham treatment) or one of the following hormones: aldosterone (100nM), corticosterone (100nM), or T3 (100nM). While acute exposure had limited effects on respiratory motor output, chronic incubation with any hormone significantly increased fictive air breathing; the burst frequencies observed following treatment were similar to those observed in adult bullfrogs. We conclude that through their long term effects, hormones regulating metamorphosis can initiate the maturation of the neural circuits that generate and regulate breathing in this species.
authors.affiliation: Département de Pédiatrie, Université Laval, Centre de Recherche du CHU de Québec, Hôpital St-François d'Assise, Québec, QC, Canada G1L 3L5. Electronic address: jean-philippe.rousseau.3@ulaval.ca.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Rousseau
authors.affiliation: Département de Pédiatrie, Université Laval, Centre de Recherche du CHU de Québec, Hôpital St-François d'Assise, Québec, QC, Canada G1L 3L5.
authors.forename: Aida
authors.initials: A
authors.lastname: Bairam
authors.affiliation: Département de Pédiatrie, Université Laval, Centre de Recherche du CHU de Québec, Hôpital St-François d'Assise, Québec, QC, Canada G1L 3L5.
authors.forename: Richard
authors.initials: R
authors.lastname: Kinkead
mesh_terms: D000450:Aldosterone / Q000378:metabolism; D000818:Animals; D001933:Brain Stem / Q000196:embryology* / Q000502:physiology*; D003345:Corticosterone / Q000378:metabolism; D004594:Electrophysiology; D007814:Larva / Q000502:physiology*; D008675:Metamorphosis, Biological / Q000502:physiology; D009924:Organ Culture Techniques; D011892:Rana catesbeiana / Q000196:embryology* / Q000502:physiology; D012143:Respiratory Physiological Phenomena; D013963:Thyroid Hormones / Q000378:metabolism
chemical_list: D013963:Thyroid Hormones; D000450:Aldosterone; D003345:Corticosterone
keywords: Brain stem; Bullfrog; CO(2)/pH chemoreceptor; Control of breathing; Development; HPA axis