title: Extracellular and cell-associated forms of Gluconobacter oxydans dextran dextrinase change their localization depending on the cell growth.
abstract: Gluconobacter oxydans ATCC 11894 produces dextran dextrinase (DDase, EC 2.4.1.2), which synthesizes dextran from the starch hydrolysate, dextrin and is known to cause ropy beer. G. oxydans ATCC 11894 was believed to possess both a secreted DDase (DDext) and an intracellular DDase (DDint), expressed upon cultivation with dextrin and glucose, respectively. However, genomic Southern blot, peptide mass fingerprinting and reaction product-pattern analyses revealed that both DDext and DDint were identical. The activity in the cell suspension and its liberation from the spheroplast cells indicated that DDint was localized on the cell surface. The localization of DDase was altered during the culture depending on the growth phase. During the early growth stage, DDase was exclusively liberated into the medium (DDext), and the cell-associated form (DDint) appeared after depletion of glucose from the medium.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Juri
authors.initials: J
authors.lastname: Sadahiro
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Haruhide
authors.initials: H
authors.lastname: Mori
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saburi
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Okuyama
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Electronic address: kimura@abs.agr.hokudai.ac.jp.
authors.forename: Atsuo
authors.initials: A
authors.lastname: Kimura
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002384:Catalysis; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D003470:Culture Media; D003911:Dextrans / Q000737:chemistry; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D020600:Gluconobacter oxydans / Q000201:enzymology*; D005947:Glucose / Q000737:chemistry; D005964:Glucosyltransferases / Q000378:metabolism*; D010449:Peptide Mapping; D011994:Recombinant Proteins / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D003911:Dextrans; D011994:Recombinant Proteins; D005964:Glucosyltransferases; C402863:dextrin dextranase; D005947:Glucose
keywords: Dextran; Dextran dextrinase; Gluconobacter oxydans; Outer membrane; Secretion; Subcellular localization