title: Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury.
abstract: This study aimed to investigate the effects of Compound Ginkgo biloba (CGB) on alterations in intestinal permeability and inflammation caused by endotoxin in chronic alcohol-induced liver injury. CGB was prepared by Ginkgo biloba extract and Rosa roxburghii in a 1 : 1 proportion. Rats were divided into four groups: control, ethanol, high-dosage CGB (0.6 g kg(-1) d(-1)) and low-dosage CGB (0.2 g kg(-1) d(-1)) group. Rats in the control group ingested a Lieber-DeCarli control liquid diet, while rats in the ethanol and CGB-treated groups ingested a Lieber-DeCarli alcohol liquid diet for eight weeks. CGB was orally administered from the beginning of the third week until the end of the experiment. CGB was observed to significantly reduce the activities of serum ALT, AST, diamine oxidase (DAO) as well as levels of serum TG, D-lactic acid and plasma endotoxin in rats fed with Lieber-DeCarli ethanol liquid. Further, the hepatic steatosis was improved and the damage to intestinal tight junctions was also relieved effectively after CGB administration. Moreover, CGB significantly downregulated the expressions of TNF-α, lipopolysaccharide binding protein (LBP), CD14 and TLR4 in the liver and upregulated the expressions of tight junction proteins including ZO-1, occludin and claudin-1. In summary, this study demonstrated that CGB alleviated alcohol-induced liver injury and hepatic lipopolysaccharide signaling as well as gut barrier dysfunction through restoring tight junctions.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, 138 Xianlin Road, Qixia District, Nanjing 210023, P. R. China. 13851664089@163.com.
authors.forename: Huanzhou
authors.initials: H
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Qiu
authors.forename: Juanhong
authors.initials: J
authors.lastname: Wang
authors.forename: Congcong
authors.initials: C
authors.lastname: Niu
authors.forename: Suhua
authors.initials: S
authors.lastname: Pan
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics / Q000378:metabolism; D000410:Alanine Transaminase / Q000097:blood; D006631:Amine Oxidase (Copper-Containing) / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D062445:Claudin-1 / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D004731:Endotoxins / Q000097:blood; D000431:Ethanol / Q000009:adverse effects; D020441:Ginkgo biloba / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects; D019344:Lactic Acid / Q000097:blood; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D010539:Permeability; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027826:Rosa / Q000737:chemistry; D019108:Tight Junctions / Q000187:drug effects / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation; D062826:Zonula Occludens-1 Protein / Q000235:genetics / Q000378:metabolism; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D000410:Alanine Transaminase; D006631:Amine Oxidase (Copper-Containing); D001219:Aspartate Aminotransferases; D002352:Carrier Proteins; D062445:Claudin-1; D004731:Endotoxins; D000431:Ethanol; D019344:Lactic Acid; D018950:Lipopolysaccharide Receptors; D008562:Membrane Glycoproteins; D062793:Occludin; D010936:Plant Extracts; D051197:Toll-Like Receptor 4; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; D062826:Zonula Occludens-1 Protein; D000099231:Lipopolysaccharide-Binding Protein; C568114:Cldn1 protein, rat; C568987:Ocln protein, rat; C569044:Tjp1 protein, rat; C495347:Tlr4 protein, rat