title: Olive oil and its phenolic constituent tyrosol attenuates dioxin-induced toxicity in peripheral blood mononuclear cells via an antioxidant-dependent mechanism.
abstract: Olive oil (OO) and its phenolic compounds are reported to possess many potential biological effects, which are ascribed to its powerful antioxidant property. In this study, we have assessed whether OO and its phenolic compound tyrosol (TY) could mitigate 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced oxidative damages in peripheral blood mononuclear cells (PBMC). The results showed that exposure of PBMC to 10 nM TCDD caused significant cell death and elevated cellular concentrations of reactive oxygen species and lipid peroxidation. Comet assay indicated that OO and TY protected DNA damage against dioxin toxicity. In addition, alterations in levels of antioxidant enzymes were substantially prevented by OO and TY. TCDD-induced CYP1A1 activity and loss of mitochondrial membrane potential were significantly reduced by the administration of OO and TY. The results suggested that dietary modifications incorporating diets rich in OO and associated phenolics could prove beneficial in protecting individuals against toxicity induced by dioxins.
authors.affiliation: a Department of Biotechnology , Alagappa University , Karaikudi 630 004 , Tamil Nadu , India.
authors.forename: Ilavarasi
authors.initials: I
authors.lastname: Kalaiselvan
authors.affiliation: a Department of Biotechnology , Alagappa University , Karaikudi 630 004 , Tamil Nadu , India.
authors.forename: Sheeja Malar
authors.initials: SM
authors.lastname: Dicson
authors.affiliation: a Department of Biotechnology , Alagappa University , Karaikudi 630 004 , Tamil Nadu , India.
authors.forename: Pandima Devi
authors.initials: PD
authors.lastname: Kasi
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D015227:Lipid Peroxidation; D053078:Membrane Potential, Mitochondrial; D000069463:Olive Oil / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D010636:Phenols; D000072317:Polychlorinated Dibenzodioxins; D017382:Reactive Oxygen Species; C011867:4-hydroxyphenylethanol; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; D010626:Phenylethyl Alcohol
keywords: TCDD; comet assay; olive oil; oxidative stress; peripheral blood mononuclear cells; tyrosol