title: Enzymatic and acidic degradation effect on intracellular polysaccharide of Flammulina velutipes SF-08.
abstract: The intracellular polysaccharide (IPS) from Flammulina velutipes SF-08 mycelia was isolated and degraded by enzyme and acid. IPS and its derivative were purified by DEAE-52 cellulose chromatography, and five fractions were obtained. The structural features and antioxidant activities in vitro of the isolated fractions were evaluated. On the basis of chemical composition and antioxidant ability analyses, rhamnose as the main monosaccharide might contribute to the strongest antioxidant capacity. The in vivo results showed that IPS significantly enhanced the activities of anti-aging enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and reduced the content of lipid peroxidantion (LPO). These results suggested that IPS should be a potent natural polymer and can be developed to be novel functional food.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Shandong Agricultural Technology Extending Station, Jinan, Shandong 250100, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Cui
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Second High School of Taian, Taian, Shandong 271000, PR China.
authors.forename: Mengshi
authors.initials: M
authors.lastname: Jia
authors.affiliation: College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: shjia@sdau.edu.cn.
authors.forename: Shouhua
authors.initials: S
authors.lastname: Jia
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, PR China. Electronic address: ourlaboratory@163.com.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000367:Age Factors; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002384:Catalysis; D004305:Dose-Response Relationship, Drug; D055393:Flammulina / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D006868:Hydrolysis; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Enzymolysis and acidolysis; Flammulina velutipes SF-08; Intracellular polysaccharide