title: Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model.
abstract: Local recurrence is a major clinical issue following surgical resection in head and neck cancer, and the dissemination and lymph node metastasis of minimal residual disease is relatively difficult to treat due to the lack of suitable therapeutic approaches. In the present study, we developed and evaluated a novel immunotherapy using a skin flap transfer treated with sensitized dendritic cells (DC), termed the "immuno-flap," in a rat tumor model. After the local round area of skin was resected, SCC-158 cells (a rat head and neck cancer cell line) were inoculated into the muscle surface; lastly, the groin skin flap injected with mature DC was overlaid. Two weeks after the second DC injection, systemic immunological reactions and tumor size were measured. The DC-treated group showed a significant reduction in tumor size compared with the control. Although the induction of CTL activity in spleen cells was marginal, Th1 cytokines such as interleukin-2 and interferon-γ were elevated in the DC-treated group. These results suggest that a novel immunotherapy based on the immuno-flap method has the potential for clinical application to prevent the local recurrence of head and neck cancer patients.
authors.affiliation: Division of Plastic and Reconstructive Surgery, Shizuoka Cancer Center Hospital, Nagaizumi, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Inoue
authors.forename: Noriko
authors.initials: N
authors.lastname: Saegusa
authors.forename: Maho
authors.initials: M
authors.lastname: Omiya
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ashizawa
authors.forename: Haruo
authors.initials: H
authors.lastname: Miyata
authors.forename: Masaru
authors.initials: M
authors.lastname: Komiyama
authors.forename: Akira
authors.initials: A
authors.lastname: Iizuka
authors.forename: Akiko
authors.initials: A
authors.lastname: Kume
authors.forename: Takashi
authors.initials: T
authors.lastname: Sugino
authors.forename: Ken
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kiyohara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Akiyama
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D006258:Head and Neck Neoplasms / Q000276:immunology*; D007167:Immunotherapy / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D009364:Neoplasm Recurrence, Local / Q000276:immunology; D051381:Rats; D012867:Skin / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology
chemical_list: D019496:Cancer Vaccines; D007376:Interleukin-2; D007371:Interferon-gamma
keywords: Dendritic cell; head and neck cancer; immuno-flap; local recurrence; rat model