title: Genotoxicity, potential cytotoxicity and cell uptake of titanium dioxide nanoparticles in the marine fish Trachinotus carolinus (Linnaeus, 1766).
abstract: Nanoparticles have physicochemical characteristics that make them useful in areas such as science, technology, medicine and in products of everyday use. Recently the manufacture and variety of these products has grown rapidly, raising concerns about their impact on human health and the environment. Adverse effects of exposure to nanoparticles have been reported for both terrestrial and aquatic organisms, but the toxic effects of the substances on marine organisms remain poorly understood. The main aim of this study was to evaluate the genotoxicity of TiO2-NP in the marine fish Trachinotus carolinus, through cytogenotoxic methods. The fish received two different doses of 1.5 μg and 3.0 μg-TiO2-NP g(-1) by intraperitoneal injection. Blood samples were collected to analyze erythrocyte viability using the Trypan Blue exclusion test, comet assay (pH>13), micronucleus (MN) and other erythrocyte nuclear abnormalities (ENA) 24, 48 and 72 h after injection. The possible cell uptake of TiO2-NP in fish injected with the higher dose was investigated after 72 h using transmission electron microscopy (TEM). The results showed that TiO2-NP is genotoxic and potentially cytotoxic for this species, causing DNA damage, inducing the formation of MN and other ENA, and decreasing erythrocyte viability. TEM examination revealed that cell uptake of TiO2-NP was mainly in the kidney, liver, gills and to a lesser degree in muscle. To the extent of the authors' knowledge, this is the first in vivo study of genotoxicity and other effects of TiO2-NP in a marine fish.
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: carolpatvig@usp.br.
authors.forename: Caroline P
authors.initials: CP
authors.lastname: Vignardi
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: humbigutis@gmail.com.
authors.forename: Fabio M
authors.initials: FM
authors.lastname: Hasue
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: pri.sartorio@gmail.com.
authors.forename: Priscila V
authors.initials: PV
authors.lastname: Sartório
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: camargonato@gmail.com.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Cardoso
authors.affiliation: Faculty of Veterinary Medicine, Integrated College North of Minas Osmane Barbosa Avenue, 11111, JK, Montes Claros, MG 39404006, Brazil. Electronic address: mamiferomarinho@gmail.com.
authors.forename: Alex S D
authors.initials: AS
authors.lastname: Machado
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: zezemar@usp.br.
authors.forename: Maria J A C R
authors.initials: MJ
authors.lastname: Passos
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: tcsantos@yahoo.com.
authors.forename: Thais C A
authors.initials: TC
authors.lastname: Santos
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: junucci@gmail.com.
authors.forename: Juliana M
authors.initials: JM
authors.lastname: Nucci
authors.affiliation: Institute of Chemistry, University of São Paulo, Prof. Lineu Prestes Avenue, 748, Cidade Universitária, Butantã, São Paulo, SP 05508000, Brazil. Electronic address: hewer@iq.usp.br.
authors.forename: Thiago L R
authors.initials: TL
authors.lastname: Hewer
authors.affiliation: Department of Anatomy, Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 2415, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: watanabe@icb.usp.br.
authors.forename: Ii-Sei
authors.initials: IS
authors.lastname: Watanabe
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: vicgomes@usp.br.
authors.forename: Vicente
authors.initials: V
authors.lastname: Gomes
authors.affiliation: Department of Biological Oceanography, Oceanographic Institute, University of São Paulo, Praça do Oceanogáfico 191, Cidade Universitária, Butantã, São Paulo, SP 05508900, Brazil. Electronic address: phanvn@usp.br.
authors.forename: Ngan V
authors.initials: NV
authors.lastname: Phan
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects*; D005399:Fishes / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000378:metabolism* / Q000633:toxicity*; D014025:Titanium / Q000378:metabolism* / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014874:Water Pollutants, Chemical; C009495:titanium dioxide; D014025:Titanium
keywords: Cytogenotoxicity; Marine fish; Nanotoxicology; TiO(2)