title: Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer.
abstract: Lung cancer is the second most common type of cancer in the world and is the most common cause of cancer-related death in both men and women. Research into causes, prevention and treatment of lung cancer is ongoing and much progress has been made recently in these areas, however survival rates have not significantly improved. Therefore, it is essential to develop biomarkers for early diagnosis of lung cancer, prediction of metastasis and evaluation of treatment efficiency, as well as using these molecules to provide some understanding about tumour biology and translate highly promising findings in basic science research to clinical application. In this investigation, two-dimensional difference gel electrophoresis and mass spectrometry were initially used to analyse conditioned media from a panel of lung cancer and normal bronchial epithelial cell lines. Significant proteins were identified with heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1), pyruvate kinase M2 isoform (PKM2), Hsc-70 interacting protein and lactate dehydrogenase A (LDHA) selected for analysis in serum from healthy individuals and lung cancer patients. hnRNPA2B1, PKM2 and LDHA were found to be statistically significant in all comparisons. Tissue analysis and knockdown of hnRNPA2B1 using siRNA subsequently demonstrated both the overexpression and potential role for this molecule in lung tumorigenesis. The data presented highlights a number of in vitro derived candidate biomarkers subsequently verified in patient samples and also provides some insight into their roles in the complex intracellular mechanisms associated with tumour progression.
authors.affiliation: National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
authors.forename: Paul
authors.initials: P
authors.lastname: Dowling
authors.forename: Damian
authors.initials: D
authors.lastname: Pollard
authors.forename: AnneMarie
authors.initials: A
authors.lastname: Larkin
authors.forename: Michael
authors.initials: M
authors.lastname: Henry
authors.forename: Paula
authors.initials: P
authors.lastname: Meleady
authors.forename: Kathy
authors.initials: K
authors.lastname: Gately
authors.forename: Kenneth
authors.initials: K
authors.lastname: O'Byrne
authors.forename: Martin P
authors.initials: MP
authors.lastname: Barr
authors.forename: Vincent
authors.initials: V
authors.lastname: Lynch
authors.forename: Jo
authors.initials: J
authors.lastname: Ballot
authors.forename: John
authors.initials: J
authors.lastname: Crown
authors.forename: Michael
authors.initials: M
authors.lastname: Moriarty
authors.forename: Emmet
authors.initials: E
authors.lastname: O'Brien
authors.forename: Ross
authors.initials: R
authors.lastname: Morgan
authors.forename: Martin
authors.initials: M
authors.lastname: Clynes
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020543:Proteome; D040901:Proteomics
chemical_list: D014408:Biomarkers, Tumor; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D020543:Proteome; C439768:hnRNP A2