title: Rapid and sensitive detection of foodborne pathogenic bacteria (Staphylococcus aureus) using an electrochemical DNA genomic biosensor and its application in fresh beef.
abstract: Rapid early detection of food contamination is the main key in food safety and quality control. Biosensors are emerging as a vibrant area of research, and the use of DNA biosensor recognition detectors is relatively new. In this study a genomic DNA biosensor system with a fixing and capture probe was modified by a sulfhydryl and amino group, respectively, as complementary with target DNA. After immobilization and hybridization, the following sandwich structure fixing DNA-target DNA-capture DNA-PbS NPs was formed to detect pathogenic bacteria (Staphylococuus aureus EF529607.1) by using GCE modified with (multiwalled carbon nanotubes-chitosan-bismuth) to increase the sensitivity of the electrode. The modification procedure was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. The sandwich structure was dissolved in 1 M nitric acid to become accessible to the electrode, and the PbS NPs was measured in solution by differential pulse voltammetry (DPV). The results showed that the detection limit of the DNA sensor was 3.17 × 10(-14) M S. aureus using PbS NPs, whereas the result for beef samples was 1.23 ng/mL. Thus, according to the experimental results presented, the DNA biosensor exhibited high sensitivity and rapid response, and it will be useful for the food matrix.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University , Wuxi, Jiangsu 214122, China.
authors.forename: Mandour H
authors.initials: MH
authors.lastname: Abdalhai
authors.forename: António Maximiano
authors.initials: AM
authors.lastname: Fernandes
authors.forename: Mohand
authors.initials: M
authors.lastname: Bashari
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.forename: Qian
authors.initials: Q
authors.lastname: He
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial
keywords: DNA electrochemistry biosensor; MWCNT-Chi-Bi; Staphylococcus aureus; foodborne pathogenic; fresh beef