title: Functional analysis of related CrRLK1L receptor-like kinases in pollen tube reception.
abstract: The Catharanthus roseus Receptor-Like Kinase 1-like (CrRLK1L) family of 17 receptor-like kinases (RLKs) has been implicated in a variety of signaling pathways in Arabidopsis, ranging from pollen tube (PT) reception and tip growth to hormonal responses. The extracellular domains of these RLKs have malectin-like domains predicted to bind carbohydrate moieties. Domain swap analysis showed that the extracellular domains of the three members analyzed (FER, ANX1, HERK1) are not interchangeable, suggesting distinct upstream components, such as ligands and/or co-factors. In contrast, their intercellular domains are functionally equivalent for PT reception, indicating that they have common downstream targets in their signaling pathways. The kinase domain is necessary for FER function, but kinase activity itself is not, indicating that other kinases may be involved in signal transduction during PT reception.
authors.affiliation: Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA sakessler@ou.edu grossnik@botinst.uzh.ch.
authors.forename: Sharon A
authors.initials: SA
authors.lastname: Kessler
authors.affiliation: Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland.
authors.forename: Heike
authors.initials: H
authors.lastname: Lindner
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
authors.forename: Daniel S
authors.initials: DS
authors.lastname: Jones
authors.affiliation: Institute of Plant Biology & Zürich-Basel Plant Science Center, University of Zürich, Zürich, Switzerland sakessler@ou.edu grossnik@botinst.uzh.ch.
authors.forename: Ueli
authors.initials: U
authors.lastname: Grossniklaus
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism*; D005816:Genetic Complementation Test; D009154:Mutation; D010770:Phosphotransferases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D053205:Pollen Tube / Q000235:genetics / Q000378:metabolism*; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D015398:Signal Transduction; D017346:Protein Serine-Threonine Kinases
chemical_list: D029681:Arabidopsis Proteins; D010770:Phosphotransferases; D011494:Protein Kinases; C546340:ANXUR1 protein, Arabidopsis; C522131:At3g51550 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases; C540877:HERCULES1 protein, Arabidopsis
keywords: Arabidopsis; FERONIA; pollen tube reception; receptor‐like kinases; signaling