title: Antibacterial activity of microstructured Ag/Au sacrificial anode thin films.
abstract: Ten different Ag dot arrays (16 to 625 microstructured dots per square mm) were fabricated on a continuous Au thin film and for comparison also on Ti film by sputter deposition and photolithographic patterning. To analyze the antibacterial activity of these microstructured films Escherichia coli and Staphylococcus aureus were placed onto the array surfaces and cultivated overnight. To analyze the viability of planktonic as well as surface adherent bacteria, the applied bacterial fluid was subsequently aspirated, plated on blood agar plates and adherent bacteria were detected by fluorescence microscopy. A particular antibacterial effect towards both bacterial strains was induced by Ag dot arrays on fabricated Au thin film (sacrificial anode system for Ag), due to the release of Ag ions from dissolution of Ag dots in contrast to Ag dot arrays fabricated on the Ti thin films (non-sacrificial anode system for Ag) which remained intact to the original dot shape. The required number of Ag dots on gold film to achieve complete bactericidal effects for both bacterial strains was seven times lower than that observed with Ag dot arrays on Ti film.
authors.affiliation: Surgical Research, BG University Hospital Bergmannsheil, Buerkle-de-la-Camp-Platz 1, 44789 Bochum, Germany. Electronic address: Manfred.Koeller@rub.de.
authors.forename: Manfred
authors.initials: M
authors.lastname: Köller
authors.affiliation: Surgical Research, BG University Hospital Bergmannsheil, Buerkle-de-la-Camp-Platz 1, 44789 Bochum, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Sengstock
authors.affiliation: Institute for Materials, Faculty of Mechanical Engineering, Ruhr-University, Universitätsstraße 150, 44801 Bochum, Germany.
authors.forename: Yahya
authors.initials: Y
authors.lastname: Motemani
authors.affiliation: Institute for Materials, Faculty of Mechanical Engineering, Ruhr-University, Universitätsstraße 150, 44801 Bochum, Germany.
authors.forename: Chinmay
authors.initials: C
authors.lastname: Khare
authors.affiliation: Institute for Materials, Faculty of Mechanical Engineering, Ruhr-University, Universitätsstraße 150, 44801 Bochum, Germany.
authors.forename: Pio J S
authors.initials: PJ
authors.lastname: Buenconsejo
authors.affiliation: Institute for Materials, Faculty of Mechanical Engineering, Ruhr-University, Universitätsstraße 150, 44801 Bochum, Germany.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Geukes
authors.affiliation: Surgical Research, BG University Hospital Bergmannsheil, Buerkle-de-la-Camp-Platz 1, 44789 Bochum, Germany.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Schildhauer
authors.affiliation: Institute for Materials, Faculty of Mechanical Engineering, Ruhr-University, Universitätsstraße 150, 44801 Bochum, Germany.
authors.forename: Alfred
authors.initials: A
authors.lastname: Ludwig
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002478:Cells, Cultured; D004566:Electrodes; D006046:Gold / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D006046:Gold
keywords: Antibacterial surface; Gold; Sacrificial anode; Silver; Thin film