title: Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO₂ nanofibrous surfaces.
abstract: Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies.
authors.affiliation: Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Ai Wen
authors.initials: AW
authors.lastname: Tan
authors.affiliation: Department of Physiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia.
authors.forename: Lelia
authors.initials: L
authors.lastname: Tay
authors.affiliation: Department of Physiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, Malaysia.
authors.forename: Kien Hui
authors.initials: KH
authors.lastname: Chua
authors.affiliation: Department of Mechanical Engineering, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Roslina
authors.initials: R
authors.lastname: Ahmad
authors.affiliation: Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, USA.
authors.forename: Sheikh Ali
authors.initials: SA
authors.lastname: Akbar
authors.affiliation: Department of Biomedical Engineering, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Pingguan-Murphy
mesh_terms: D017667:Adipocytes / Q000187:drug effects*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002468:Cell Physiological Phenomena / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D039904:Pluripotent Stem Cells / Q000187:drug effects*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D018797:Cell Cycle Proteins; C009495:titanium dioxide; D014025:Titanium
keywords: nanofibers; pluripotency; stem cells; thermal oxidation; titania