title: Polydatin prevents angiotensin II-induced cardiac hypertrophy and myocardial superoxide generation.
abstract: Our studies and others recently demonstrate that polydatin, a resveratrol glucoside, has antioxidative and cardioprotective effects. This study aims to investigate the direct effects of polydatin on Ang II-induced cardiac hypertrophy to explore the potential role of polydatin in cardioprotection. Our results showed that in primary cultured cardiomyocytes, polydatin blocked Ang II-induced cardiac hypertrophy in a dose-dependent manner, which were associated with reduction in the cell surface area and [(3)H]leucine incorporation, as well as attenuation of the mRNA expressions of atrial natriuretic factor and β-myosin heavy chain. Furthermore, polydatin prevented rat cardiac hypertrophy induced by Ang II infusion, as assessed by heart weight-to-body weight ratio, cross-sectional area of cardiomyocyte, and gene expression of hypertrophic markers. Further investigation demonstrated that polydatin attenuated the Ang II-induced increase in the reactive oxygen species levels and NADPH oxidase activity in vivo and in vitro. Polydatin also blocked the Ang II-stimulated increases of Nox4 and Nox2 expression in cultured cardiomyocytes and the hearts of Ang II-infused rats. Our results indicate that polydatin has the potential to protect against Ang II-mediated cardiac hypertrophy through suppression of NADPH oxidase activity and superoxide production. These observations may shed new light on the understanding of the cardioprotective effect of polydatin.
authors.affiliation: Medical Research Center, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China zhangndsu@yahoo.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Medical Research Center, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Medical Research Center, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi 712046, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology & Toxicology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA.
authors.forename: Fanrong
authors.initials: F
authors.lastname: Yao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism; D001835:Body Weight; D006332:Cardiomegaly / Q000139:chemically induced / Q000517:prevention & control*; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000187:drug effects; D019255:NADPH Oxidases / Q000378:metabolism; D009929:Organ Size; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*; D013481:Superoxides / Q000378:metabolism*; D024723:Ventricular Myosins / Q000378:metabolism
chemical_list: D005960:Glucosides; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013267:Stilbenes; D013481:Superoxides; D000804:Angiotensin II; D009320:Atrial Natriuretic Factor; D019255:NADPH Oxidases; D024723:Ventricular Myosins; D000077185:Resveratrol; C058229:polydatin
keywords: NADPH oxidase; Polydatin; angiotensin II; cardiac hypertrophy; reactive oxygen species