title: The value of mechanistic biophysical information for systems-level understanding of complex biological processes such as cytokinesis.
abstract: This review illustrates the value of quantitative information including concentrations, kinetic constants and equilibrium constants in modeling and simulating complex biological processes. Although much has been learned about some biological systems without these parameter values, they greatly strengthen mechanistic accounts of dynamical systems. The analysis of muscle contraction is a classic example of the value of combining an inventory of the molecules, atomic structures of the molecules, kinetic constants for the reactions, reconstitutions with purified proteins and theoretical modeling to account for the contraction of whole muscles. A similar strategy is now being used to understand the mechanism of cytokinesis using fission yeast as a favorable model system.
authors.affiliation: Departments of Molecular Cellular and Developmental Biology, Molecular Biophysics and Biochemistry, and Cell Biology, Yale University, New Haven, Connecticut. Electronic address: thomas.pollard@yale.edu.
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Pollard
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D048749:Cytokinesis; D056890:Eukaryota / Q000502:physiology; D006801:Humans; D008954:Models, Biological; D009119:Muscle Contraction / Q000502:physiology; D049490:Systems Biology