title: Characterization of mesenchymal stem cells derived from rat bone marrow and adipose tissue: a comparative study.
abstract: OBJECTIVE
  Stem cell technology offers a new hope for many chronic disorders patients. The types of stem cells are different with many differences existing between each type. Mesenchymal stem cells (MSCs) represent one type of adult stem cells that can be easily isolated, then re-transplanted to the patients. This offers potential for their future application in treating many disorders without fear of rejection possibility. MSCs can be isolated from different sources e.g. bone marrow (BMSCs) and adipose tissue (ADSCs). In the present study we compared BMSCs and ADSCs isolated from Sprague-Dawley rats.
  
  
  RESULTS
  For this comparison, immunophenotyping, the analysis of growth rates, proliferation by colony forming unit-fibroblast assay, population doubling time, and trilineage differentiation assays were performed for both BMSCs and ADSCs. The findings revealed that despite no difference in immunphenotypic character between BMSC and ADSC, a better proliferative capacity was observed for ADSCs which would advocate their better use in regenerative applications. On the other hand, BMSCs showed more potential for osteogenic and chondrogenic differentiation.
  
  
  CONCLUSIONS
  Our study showed that, despite many similarities between both types of cells, there are differences existing which can offer assistance on choosing type of cell to be used in specific diseases. Although ADSCs seem more promising for regenerative application generally, BMSCs may represent a better choice for treating bone disorders.
authors.affiliation: Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura ; Center of Excellence for Stem Cells and Regenerative Medicine (CESCRM), Zewail City of Science and Technology.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lotfy
authors.affiliation: Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Salama
authors.affiliation: Biochemistry Department, Faculty of Science, Zagazig University, Egypt.
authors.forename: Faten
authors.initials: F
authors.lastname: Zahran
authors.affiliation: Leeds Institute of Rheumatic and Musculskeletal Medicine, University of Leeds, Leeds, UK.
authors.forename: Elena
authors.initials: E
authors.lastname: Jones
authors.affiliation: Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Badawy
authors.affiliation: Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sobh
keywords: ADSCs; BMSCs; Bone Marrow; Characterization; Mesenchymal Stem Cells; Sprague-Dawley Rats