title: Blocking junctional adhesion molecule C enhances dendritic cell migration and boosts the immune responses against Leishmania major.
abstract: The recruitment of dendritic cells to sites of infections and their migration to lymph nodes is fundamental for antigen processing and presentation to T cells. In the present study, we showed that antibody blockade of junctional adhesion molecule C (JAM-C) on endothelial cells removed JAM-C away from junctions and increased vascular permeability after L. major infection. This has multiple consequences on the output of the immune response. In resistant C57BL/6 and susceptible BALB/c mice, we found higher numbers of innate immune cells migrating from blood to the site of infection. The subsequent migration of dendritic cells (DCs) from the skin to the draining lymph node was also improved, thereby boosting the induction of the adaptive immune response. In C57BL/6 mice, JAM-C blockade after L. major injection led to an enhanced IFN-γ dominated T helper 1 (Th1) response with reduced skin lesions and parasite burden. Conversely, anti JAM-C treatment increased the IL-4-driven T helper 2 (Th2) response in BALB/c mice with disease exacerbation. Overall, our results show that JAM-C blockade can finely-tune the innate cell migration and accelerate the consequent immune response to L. major without changing the type of the T helper cell response.
authors.affiliation: Department of Pathology and Immunology, Centre Médical Universitaire, University of Geneva, Geneva, Switzerland.
authors.forename: Romain
authors.initials: R
authors.lastname: Ballet
authors.affiliation: Department of Pathology and Immunology, Centre Médical Universitaire, University of Geneva, Geneva, Switzerland.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Emre
authors.affiliation: Department of Pathology and Immunology, Centre Médical Universitaire, University of Geneva, Geneva, Switzerland.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Jemelin
authors.affiliation: Department of Biochemistry, and WHO Immunology Research and Training Center, University of Lausanne, Epalinges, Switzerland.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Charmoy
authors.affiliation: Department of Biochemistry, and WHO Immunology Research and Training Center, University of Lausanne, Epalinges, Switzerland.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Tacchini-Cottier
authors.affiliation: Department of Pathology and Immunology, Centre Médical Universitaire, University of Geneva, Geneva, Switzerland.
authors.forename: Beat A
authors.initials: BA
authors.lastname: Imhof
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D005260:Female; D007111:Immunity, Cellular; D007113:Immunity, Innate; D007136:Immunoglobulins / Q000276:immunology*; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D018417:Th1 Cells / Q000276:immunology / Q000473:pathology; D018418:Th2 Cells / Q000276:immunology / Q000473:pathology
chemical_list: D015815:Cell Adhesion Molecules; D007136:Immunoglobulins; C486723:Jam3 protein, mouse