title: Development of an in vitro model for the analysis of bovine endometrium using simple techniques.
abstract: This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero-spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells, except the glandular-like structure of the epithelial cells inside the explants. Gelatin zymography revealed that the hetero-spheroids did not express matrix metalloproteinases (MMPs) after 4 days of culture, but strong MMP expressions were observed in the cultured explants until 7 days of culture. Additionally, expression of progesterone receptor (PR), estrogen receptor alpha (ERα), type I interferon receptor 1 (IFNAR1) and 2 (IFNAR2) messenger RNA was observed both in the homo- and hetero-spheroids. The expression of oxytocin receptor (OTR) mRNA in E2 and E2+P4 (1,3,5(10)-Estratrien-3, 17β-diol + 4-Pregnen-3, 20-dinone) treated groups were significantly (P < 0.05) higher than that of the control group of spheroids. In case of cultured explants, the expression of PR and OTR mRNA were significantly (P < 0.05) higher in E2 treated groups compared to the control groups. Hepatocyte growth factor (HGF) mRNA expression was also higher in P4 treated groups at 10 days in culture (P < 0.05). In a nutshell, the in vitro model developed in this study for the analysis of the endometrium may provide a new platform for extensive research on bovine endometrial function.
authors.affiliation: Department of Animal and Marine Bioresource Sciences, Graduate School Kyushu University, Fukuoka, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamauchi
authors.forename: Kazuki
authors.initials: K
authors.lastname: Yamagami
authors.forename: Nobuhisa
authors.initials: N
authors.lastname: Nakamura
authors.forename: Seiya
authors.initials: S
authors.lastname: Yamashita
authors.forename: Md Rashedul
authors.initials: MR
authors.lastname: Islam
authors.forename: Shoji
authors.initials: S
authors.lastname: Tabata
authors.forename: Kanji
authors.initials: K
authors.lastname: Yahiro
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tamura
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hashizume
authors.forename: Masa-Aki
authors.initials: MA
authors.lastname: Hattori
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017471:Receptors, Interferon / Q000235:genetics / Q000378:metabolism; D018045:Receptors, Oxytocin / Q000235:genetics / Q000378:metabolism; D011980:Receptors, Progesterone / Q000235:genetics / Q000378:metabolism; D018874:Spheroids, Cellular / Q000378:metabolism; D013194:Staining and Labeling
chemical_list: D047628:Estrogen Receptor alpha; D012333:RNA, Messenger; D017471:Receptors, Interferon; D018045:Receptors, Oxytocin; D011980:Receptors, Progesterone; D004958:Estradiol; D017228:Hepatocyte Growth Factor; D020782:Matrix Metalloproteinases
keywords: bovine; cell culture; endometrial explants; endometrium; hetero-spheroid