title: Genetic polymorphisms of glutathione S-transferase M1 and T1, and evaluation of oxidative stress in patients with non-small cell lung cancer.
abstract: BACKGROUND
  Our objective is to investigate the genetic polymorphisms of the glutathione S-transferase M1 and T1 genes (GSTM1 and GSTT1) and evaluate oxidative damage in patients with non-small lung cancer (N-SCLC).
  
  
  METHODS
  One hundred and ten patients with N-SCLC and 100 controls are included in this case-control study. Multiplex polymerase chain reaction (PCR) analyses were used to identify the genotypes. The activities of malondialdehyde (MDA) and nitric oxide (NO) and total antioxidant capacity (T-AOC) were detected by spectroscopic analysis.
  
  
  RESULTS
  The frequencies of the GSTM1, T1, and GSTM1/T1 null genotypes in the patient group were significantly higher than that in the control group (OR = 2.071, P = 0.009; OR = 1.900, P = 0.024; OR = 3.258, P = 0.003). The activities of MDA and NO were significantly higher in the patient group than that in the control group (P <0.001), and T-AOC was significantly lower in patient group than that in control group (P <0.001). The activities of MDA, and NO were higher but the T-AOC was lower in patients with the GSTM1, T1 and M1/T1 null genotypes than those in patients with GSTM1, T1 and M1/T1 present genotypes (P <0.001).
  
  
  CONCLUSIONS
  Our results suggest that oxidative damage may be play a important role in patients with N-SCLC, and that GSTM1 and GSTT1 null genotypes may predispose the cells of patients with N-SCLC to increased oxidative damage.
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. zhhyyn@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. dr.wuxw@gmail.com.
authors.forename: Xuwei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. xiynxi1968@163.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. alice_chenmei@163.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. aacc999@126.com.
authors.forename: Zhidong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Affiliated Yan'an Hospital of Kunming Medical University, No. 245 Renmin East Road, Kunming, 650051, China. wx200420004@hotmail.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Wei
authors.affiliation: Affiliated Hospital of Guiyang Medical College, No. 9 Beijing Road, Guiyang, 550004, China. doc.tangkf@hotmail.com.
authors.forename: Kaifa
authors.initials: K
authors.lastname: Tang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: D005982:Glutathione Transferase; C413545:glutathione S-transferase T1