title: Preparation of natural three-dimensional goat kidney scaffold for the development of bioartificial organ.
abstract: The high prevalence of end-stage kidney diseases demands new treatment strategies. Decellularization approach may provide a viable option grow organs using a regenerative medicine approach. Goat kidney was decellularized by perfusion decellularization using detergents to produce an cellular construct for kidney scaffold. After pre-treatment with anticoagulant, the decellularized scaffold was analyzed for its intact three-dimensional natural architecture and vasculature. Perfusion of decellularized kidney preserved the structure and composition of renal extra-cellular matrix and vascular structures within the scaffold. No evidence of residual cellular components was found. This approach provides a model for understanding of whole organ regeneration.
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Centre for Liver Research and Diagnostics, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Vishwakarma
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Centre for Liver Research and Diagnostics, India.
authors.forename: P G
authors.initials: PG
authors.lastname: Bhavani
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Centre for Liver Research and Diagnostics, India.
authors.forename: A
authors.initials: A
authors.lastname: Bardia
authors.affiliation: Department of Radiology, Deccan College of Medical Sciences, Kanchanbagh, India.
authors.forename: A
authors.initials: A
authors.lastname: Abkari
authors.affiliation: Department of Radiology, Deccan College of Medical Sciences, Kanchanbagh, India.
authors.forename: G S N
authors.initials: GS
authors.lastname: Murthy
authors.affiliation: Department of Radiology, Deccan College of Medical Sciences, Kanchanbagh, India.
authors.forename: J
authors.initials: J
authors.lastname: Venkateshwarulu
authors.affiliation: Department of Stem Cells and Regenerative Medicine, Centre for Liver Research and Diagnostics, India ; Department of Cell and Tissue Engineering, Salar-E-Millat Sultan Salahuddin Owaisi Centre for Cellular and Molecular Medicine, Princess Esra Hospital, Shalibanda, Hyderabad, Andhra Pradesh, India.
authors.forename: A A
authors.initials: AA
authors.lastname: Khan
keywords: Extra-cellular matrix; goat kidney; organ engineering; perfusion decellularization