title: The microRNA-367 inhibits the invasion and metastasis of gastric cancer by directly repressing Rab23.
abstract: BACKGROUND
  Dysregulated expression of microRNAs is often found in gastric cancer, and it contributes to the pathogenesis of gastric cancer via regulation of the cell cycle, proliferation, apoptosis, migration, and invasion.
  
  
  OBJECTIVE
  In this study, we aimed to investigate the role of miR-367 in the invasion and metastasis of gastric cancer.
  
  
  METHODS
  The correlation between the expression level of miR-367 and the clinicopathologic features of 37 patients with gastric cancer was analyzed by using real-time polymerase chain reaction (RT-PCR). In addition, we investigated the effect of miR-367 on the invasion and migration of the gastric cancer cell lines HS746T and SGC-7901 using transwell and scratch-wound assays, and the target gene of miR-367 was predicated and demonstrated by the bioinformatics method and luciferase reporter system, respectively.
  
  
  RESULTS
  The results showed that the expression of miR-367 was significantly reduced in the gastric cancer tissues compared with the paraneoplastic tissues, and significantly correlated with the differentiation level, tumor-node-metastasis (TNM) stage, and metastasis of gastric cancer. Notably, the overexpression of miR-367 in gastric cancer cells inhibited the cellular migration and invasion. Furthermore, the luciferase reporter system demonstrated that Rab23 was a target gene of miR-367, and ectopic expression of Rab23 could reverse the invasion and migration inhibitory activity of miR-367.
  
  
  CONCLUSIONS
  Our study shows that miR-367 is a key negative regulator of the invasion and metastasis of gastric cancer and establishes a strong rationale for developing miR-367 as a novel therapeutic agent against gastric cancer.
authors.affiliation: 1 Department of General Surgery, The First Hospital Affiliated to the Xinxiang Medical University , Xinxiang, Henan, China .
authors.forename: Zhang
authors.initials: Z
authors.lastname: Bin
authors.forename: He
authors.initials: H
authors.lastname: Dedong
authors.forename: Fang
authors.initials: F
authors.lastname: Xiangjie
authors.forename: Xu
authors.initials: X
authors.lastname: Hongwei
authors.forename: Yang
authors.initials: Y
authors.lastname: Qinghui
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000368:Aged; D002277:Carcinoma / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D004274:DNA, Recombinant / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D012334:RNA, Neoplasm / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D020691:rab GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D020413:3' Untranslated Regions; D004274:DNA, Recombinant; C568081:MIRN367 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; C486614:RAB23 protein, human; D020691:rab GTP-Binding Proteins