title: Polyoxygenated steroids from the octocoral Leptogorgia punicea and in vitro evaluation of their cytotoxic activity.
abstract: Five new polyoxygenated marine steroids-punicinols A-E (1-5)-were isolated from the gorgonian Leptogorgia punicea and characterized by spectroscopic methods (IR, MS, 1H, 13C and 2-D NMR). The five compounds induced in vitro cytotoxic effects against lung cancer A549 cells, while punicinols A and B were the most active, with IC50 values of 9.7 μM and 9.6 μM, respectively. The synergistic effects of these compounds with paclitaxel, as well as their effects on cell cycle distribution and their performance in the clonogenic assay, were also evaluated. Both compounds demonstrated significant synergistic effects with paclitaxel.
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. mizabelgm@gmail.com.
authors.forename: Maria Izabel G
authors.initials: MI
authors.lastname: Moritz
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. lucasmarostica@yahoo.com.br.
authors.forename: Lucas Lourenço
authors.initials: LL
authors.lastname: Marostica
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. ebianco@chemist.com.
authors.forename: Everson M
authors.initials: EM
authors.lastname: Bianco
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. terezarojo@gmail.com.
authors.forename: Maria Tereza R
authors.initials: MT
authors.lastname: Almeida
authors.affiliation: Invertebrates Department, National Museum, Federal University of Rio de Janeiro, Rio de Janeiro 20940-040, RJ, Brazil. joao.porifera@gmail.com.
authors.forename: João L
authors.initials: JL
authors.lastname: Carraro
authors.affiliation: UMYMFOR-Department of Organic Chemistry, FCEN, University of Buenos Aires, Buenos Aires C1428EGA, Argentina. gabyc@qo.fcen.uba.ar.
authors.forename: Gabriela M
authors.initials: GM
authors.lastname: Cabrera
authors.affiliation: UMYMFOR-Department of Organic Chemistry, FCEN, University of Buenos Aires, Buenos Aires C1428EGA, Argentina. palermo@qo.fcen.uba.ar.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Palermo
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. claudia.simoes@ufsc.br.
authors.forename: Cláudia M O
authors.initials: CM
authors.lastname: Simões
authors.affiliation: Post-Graduation Program in Pharmacy, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil. eloirschenkel@gmail.com.
authors.forename: Eloir P
authors.initials: EP
authors.lastname: Schenkel
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D004357:Drug Synergism; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017239:Paclitaxel / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D013256:Steroids; D017239:Paclitaxel