title: Expression of α4, αv, β1 and β3 integrins during the implantation window on blastocyst of a mouse model of polycystic ovarian syndromes.
abstract: BACKGROUND
  It has been hypothesized that blastocyst integrin expression changes can affect the spontaneous miscarriage in polycystic ovarian syndromes (PCOS).
  
  
  OBJECTIVE
  In this study, the profile of integrin genes and proteins was investigated on blastocyst of the PCOS experimental mouse model.
  
  
  METHODS
  30 NMRI female mice were equally divided into 3 groups: control, experimental [PCOS that was injected estradiol valerate (40 mg/kg)]. After 8 weeks, each group was hyper stimulated by PMSG and HCG. Vaginal plaque was checked, and mice were investigated 5 days after the test. Progesterone and estradiol levels were determined; α4, αv, β1 and β3 integrin genes and protein of blastocysts were examined by real time PCR method and immunohistochemistry, respectively.
  
  
  RESULTS
  Estradiol level was significantly increased (p≤0.035) in PCOS group. Based on our finding, the ratio of genes' expressions αv, β3, β1 and α4 in PCOS to control group was 0.479±0.01, 0.5±0.001, 2.7±0.4 and 1.023±0.2 respectively. Genes expression showed a great difference (p≤0.001) between β3, β1 and αv in PCOS compared to other groups. αv and β3 integrin proteins expressed in all groups but intensity of these proteins in PCOS groups, was lower than other groups.
  
  
  CONCLUSIONS
  Pattern of αv and β3 integrins expression on the mouse blastocyst surface has an important effect during the implantation window. This pattern has changed in PCOS model and might have a great influence on implantation failure. Therefore, this experimental study suggests that a great attention to this problem may be essential in patients who are involved.
authors.affiliation: Department of Anatomical Sciences, Faculty of Medical Sciences, Islamic Azad University, Yazd Branch, Yazd, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Peyghambari
authors.affiliation: Vali-e-Asr Reproductive Health Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Amanpour
authors.affiliation: Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mehri
authors.initials: M
authors.lastname: Fayazi
authors.affiliation: Vali-e-Asr Reproductive Health Research Centre, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Haddadi
authors.affiliation: Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Samad
authors.initials: S
authors.lastname: Muhammadnejad
authors.affiliation: Tumor Model Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Muhammadnejad
authors.affiliation: Department of Physiology, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Salimi
authors.affiliation: Department of Anatomical sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Mazaheri
keywords: Blastocyst; Implantation; Integrin; Polycystic ovarian syndromes