title: Molecular characterization and drug-resistance of Mycobacterium tuberculosis strains in Xuzhou, China.
abstract: To understand the genetic diversity and drug resistance status of Mycobacterium tuberculosis (M. tuberculosis) circulating in Xuzhou of China, the spacer-oligonucleotide typing (Spoligotyping) and multi-loci VNTRs (variable number tandem repeats) analysis (MLVA) were utilized for the genotyping of the isolates. Drug susceptibility test (DST) was performed by the proportion method on the Lowenstein-Jensen (L-J) medium using isoniazid, rifampicin, ethambutol, and streptomycin. By Spoligotyping, 287 M. tuberculosis isolates were differentiated into 14 clusters. Then with 15-loci MLVA, these strains could be divided into 32 clusters, 228 genotypes. Of 15 VNTRs, 6 loci had the highly discriminatory powers, 6 loci presented moderate discrimination and 3 loci demonstrated less polymorphism. The DST results showed that 46 strains were resistant to at least one first-line anti-tuberculosis agent. There was a difference in the isoniazid resistance between Beijing and non-Beijing genotype strains. We concluded that the combination of Spoligotyping and 15 VNTR loci as the genotyping in our study was applicable for this region, the drug resistant isolates were identified, and the Beijing family was the most prevalent genotype in the rural counties of Xuzhou.
authors.affiliation: Department of Immunology, Changzhi Medical College, Changzhi 046000, Shanxi, China; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Immunology, Xiangya School of Medicine, Central south University, Changsha 410078, Hunan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Pang
authors.affiliation: Xuzhou Municipal Centre for Disease Control and Prevention, Xuzhou 221006, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, Zhejiang, China.
authors.forename: Hai Can
authors.initials: HC
authors.lastname: Liu
authors.affiliation: Xuzhou Municipal Centre for Disease Control and Prevention, Xuzhou 221006, Jiangsu, China.
authors.forename: Yang Guang
authors.initials: YG
authors.lastname: Du
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, Zhejiang, China.
authors.forename: Xiu Qin
authors.initials: XQ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, Zhejiang, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Yi
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, Zhejiang, China.
authors.forename: Xiao Cui
authors.initials: XC
authors.lastname: Wu
authors.affiliation: Xuzhou Municipal Centre for Disease Control and Prevention, Xuzhou 221006, Jiangsu, China.
authors.forename: Jin Chuan
authors.initials: JC
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310000, Zhejiang, China.
authors.forename: Kang Lin
authors.initials: KL
authors.lastname: Wan
mesh_terms: D002681:China; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D060005:Genotyping Techniques; D009169:Mycobacterium tuberculosis / Q000235:genetics*