title: In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.
abstract: BACKGROUND
  Vertebrate organogenesis is a highly complex process involving sequential cascades of transcription factor activation or repression. Interestingly a single developmental control gene can occasionally be essential for the morphogenesis and differentiation of tissues and organs arising from vastly disparate embryological lineages.
  
  
  RESULTS
  Here we elucidated the role of the mammalian homeobox gene Bapx1 during the embryogenesis of five distinct organs at E12.5 - vertebral column, spleen, gut, forelimb and hindlimb - using expression profiling of sorted wildtype and mutant cells combined with genome wide binding site analysis. Furthermore we analyzed the development of the vertebral column at the molecular level by combining transcriptional profiling and genome wide binding data for Bapx1 with similarly generated data sets for Sox9 to assemble a detailed gene regulatory network revealing genes previously not reported to be controlled by either of these two transcription factors.
  
  
  CONCLUSIONS
  The gene regulatory network appears to control cell fate decisions and morphogenesis in the vertebral column along with the prevention of premature chondrocyte differentiation thus providing a detailed molecular view of vertebral column development.
authors.forename: Sumantra
authors.initials: S
authors.lastname: Chatterjee
authors.forename: V
authors.initials: V
authors.lastname: Sivakamasundari
authors.forename: Sook Peng
authors.initials: SP
authors.lastname: Yap
authors.forename: Petra
authors.initials: P
authors.lastname: Kraus
authors.forename: Vibhor
authors.initials: V
authors.lastname: Kumar
authors.forename: Xing
authors.initials: X
authors.lastname: Xing
authors.forename: Siew Lan
authors.initials: SL
authors.lastname: Lim
authors.forename: Joel
authors.initials: J
authors.lastname: Sng
authors.forename: Shyam
authors.initials: S
authors.lastname: Prabhakar
authors.affiliation: Department of Biology, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA. tlufkin@clarkson.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lufkin
mesh_terms: D000483:Alleles; D000818:Animals; D002470:Cell Survival; D019902:Chondrocytes / Q000166:cytology; D047369:Chromatin Immunoprecipitation; D004622:Embryo, Mammalian / Q000378:metabolism; D047108:Embryonic Development; D004791:Enzyme Inhibitors / Q000378:metabolism; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D016678:Genome; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D011485:Protein Binding; D055758:SOX9 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013131:Spine / Q000378:metabolism*
chemical_list: D004791:Enzyme Inhibitors; D018398:Homeodomain Proteins; D055758:SOX9 Transcription Factor