title: (1)H, (15)N and (13)C resonance assignments of the RRM1 domain of the key post-transcriptional regulator HuR.
abstract: Human antigen R (HuR) is a ubiquitous protein that recognizes adenylate and uridylate-rich elements in mRNA, thereby interfering with the fate of protein translation. This protein plays a central role in the outcome of the inflammatory response as it may stabilize or silence mRNAs of key components of the immune system. HuR is able to interact with other RNA-binding proteins, reflecting a complex network that dictates mRNAs post-transcriptional control. HuR is composed of three functional domains, known as RNA-recognition motifs (RRM1, RRM2 and RRM3). It is known that RRM1 is the most important domain for mRNA-binding affinity. In this study, we completed the NMR chemical shift assignment of the RRM1 domain of HuR, as a first step to further establishing the structure, dynamics and function relationship for this protein.
authors.affiliation: Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Mujo
authors.affiliation: Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Lixa
authors.affiliation: Department of Immunology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Letícia A M
authors.initials: LA
authors.lastname: Carneiro
authors.affiliation: Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil.
authors.forename: Cristiane D
authors.initials: CD
authors.lastname: Anobom
authors.affiliation: National Center for Nuclear Magnetic Resonance Jiri Jonas, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Fábio C
authors.initials: FC
authors.lastname: Almeida
authors.affiliation: Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, 21941-909, Brazil. pinheiro@iq.ufrj.br.
authors.forename: Anderson S
authors.initials: AS
authors.lastname: Pinheiro
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D051959:ELAV Proteins / Q000737:chemistry*; D006801:Humans; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D051959:ELAV Proteins
keywords: AU-rich elements; Assignment; Chemical shift; Human antigen R; RNA-binding Protein