title: Burkholderia monticola sp. nov., isolated from mountain soil.
abstract: An ivory/yellow, Gram-stain-negative, short-rod-shaped, aerobic bacterial strain, designated JC2948(T), was isolated from a soil sample taken from Gwanak Mountain, Republic of Korea. 16S rRNA gene sequence analysis indicated that strain JC2948(T) belongs to the genus Burkholderia. The test strain showed highest sequence similarities to Burkholderia tropica LMG 22274(T) (97.6 %), Burkholderia acidipaludis NBRC 101816(T) (97.5 %), Burkholderia tuberum LMG 21444(T) (97.5 %), Burkholderia sprentiae LMG 27175(T) (97.4 %), Burkholderia terricola LMG 20594(T) (97.3 %) and Burkholderia diazotrophica LMG 26031(T) (97.1 %). Based on average nucleotide identity (ANI) values, the new isolate represents a novel genomic species as it shows less than 90 % ANI values with other closely related species. Also, other phylosiological and biochemical comparisons allowed the phenotypic differentiation of strain JC2948(T) from other members of the genus Burkholderia. Therefore, we suggest that this strain should be classified as the type strain of a novel species of the genus Burkholderia. The name Burkholderia monticola sp. nov. (type strain, JC2948(T) = JCM 19904(T) = KACC 17924(T)) is proposed.
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Inwoo
authors.initials: I
authors.lastname: Baek
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Boram
authors.initials: B
authors.lastname: Seo
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Imchang
authors.initials: I
authors.lastname: Lee
authors.affiliation: School of Biosystems & Biomedical Science and Department of Public Health Science and the Korean University Guro Hospital, Korea University, Seoul, Republic of Korea.
authors.forename: Hana
authors.initials: H
authors.lastname: Yi
authors.affiliation: Interdisciplinary Program in Bioinformatics and the Bioinformatics Institute, Seoul National University, Seoul, Republic of Korea.|School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jongsik
authors.initials: J
authors.lastname: Chun
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002308:Cardiolipins / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D002308:Cardiolipins; D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C483858:phosphatidylethanolamine; C030778:ubiquinone 8