title: Non-covalent photo-patterning of gelatin matrices using caged collagen mimetic peptides.
abstract: To address the downside of conventional photo-patterning which can alter the chemical composition of protein scaffolds, we developed a non-covalent photo-patterning strategy for gelatin (denatured collagen) hydrogels that utilizes UV activated triple helical hybridization of caged collagen mimetic peptide (caged CMP). Here we present 2D and 3D photo-patterning of gelatin hydrogels enabled by the caged CMP derivatives, as well as creation of concentration gradients of CMPs. CMP's specificity for binding to gelatin allows patterning of almost any synthetic or natural gelatin-containing matrix, such as gelatin-methacrylate hydrogels and corneal tissues. This is a radically new tool for immobilizing drugs to natural tissues and for functionalizing scaffolds for complex tissue formation.
authors.affiliation: Department of Bioengineering, University of Utah, 36 S. Wasatch Drive, 3100 SMBB, Salt Lake City, Utah, 84112, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Boi Hoa
authors.initials: BH
authors.lastname: San
authors.forename: Julian L
authors.initials: JL
authors.lastname: Kessler
authors.forename: Jin Hwan
authors.initials: JH
authors.lastname: Kim
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Xu
authors.forename: Justin
authors.initials: J
authors.lastname: Hanes
authors.forename: Seungju Michael
authors.initials: SM
authors.lastname: Yu
mesh_terms: D032701:Biomimetics / Q000379:methods*; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods*; D005780:Gelatin / Q000032:analysis* / Q000378:metabolism; D020100:Hydrogels / Q000737:chemistry* / Q000378:metabolism; D050997:Nanomedicine / Q000379:methods* / Q000639:trends; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010777:Photochemistry / Q000379:methods; D011485:Protein Binding; D054457:Tissue Scaffolds
chemical_list: D020100:Hydrogels; D010455:Peptides; D005780:Gelatin; D003094:Collagen
keywords: hydrogel; microenvironment; spatial control; tissue engineering; triple helix