title: Screening of wild type Streptomyces isolates able to overproduce clavulanic acid.
abstract: The selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. Clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. The purpose of this work was to select the best clavulanic acid producer among strains of Streptomyces belonging to the Microorganism Collection of the Department of Antibiotics of the Federal University of Pernambuco (DAUFPE). Initially, the strains were studied for their capacity to inhibit the action of β-lactamases produced by Klebsiella aerogenes ATCC 15380. From these results, five strains were selected to investigate the batch kinetics of growth and clavulanic acid production in submerged culture carried out in flasks. The results were compared with the ones obtained by Streptomyces clavuligerus ATCC 27064 selected as a control strain. The best clavulanic acid producer was Streptomyces DAUFPE 3060, molecularly identified as Streptomyces variabilis, which increased the clavulanic acid production by 28% compared to the control strain. This work contributes to the enlargement of knowledge on new Streptomyces wild strains able to produce clavulanic acid by submerged culture.
authors.affiliation: Departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco Dois IrmãosPE Brazil Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Dois Irmãos, PE, Brazil.
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Viana Marques
authors.affiliation: Departamento de Bioprocessos e Biotecnologia Escola de Ciências Farmacêuticas Universidade Estadual Paulista "Júlio de Mesquita Filho" AraraquaraSP Brazil Departamento de Bioprocessos e Biotecnologia, Escola de Ciências Farmacêuticas, Universidade Estadual Paulista "Júlio de Mesquita Filho", Araraquara, SP, Brazil.
authors.forename: Valéria de Carvalho
authors.initials: Vde C
authors.lastname: Santos-Ebinuma
authors.affiliation: Department of Antibiotics Universidade Federal de Pernambuco, Campus Universitário RecifePE Brazil Department of Antibiotics, Universidade Federal de Pernambuco, Campus Universitário, Recife, PE, Brazil.
authors.forename: Patrícia Maria Sobral
authors.initials: PM
authors.lastname: de Oliveira
authors.affiliation: Laboratorio de Biotecnologia Universidade Federal de Pernambuco, Campus Universitário RecifePE Brazil Laboratorio de Biotecnologia, Universidade Federal de Pernambuco, Campus Universitário, Recife, PE, Brazil.
authors.forename: Gláucia Manoella de Souza
authors.initials: GM
authors.lastname: Lima
authors.affiliation: Laboratorio de Biotecnologia Universidade Federal de Pernambuco, Campus Universitário RecifePE Brazil Laboratorio de Biotecnologia, Universidade Federal de Pernambuco, Campus Universitário, Recife, PE, Brazil.
authors.forename: Janete M
authors.initials: JM
authors.lastname: Araújo
authors.affiliation: Department of Civil, Chemical and Environmental Engineering University of Genoa Genoa Italy Department of Civil, Chemical and Environmental Engineering, University of Genoa, Genoa, Italy.
authors.forename: José L
authors.initials: JL
authors.lastname: Lima-Filho
authors.affiliation: Departamento de Bioquimica e Tecnologia Farmacêutica Universidade de São Paulo São PauloSP Brazil Departamento de Bioquimica e Tecnologia Farmacêutica, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Attilio
authors.initials: A
authors.lastname: Converti
authors.affiliation: Departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco Dois IrmãosPE Brazil Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Dois Irmãos, PE, Brazil.
authors.forename: Adalberto
authors.initials: A
authors.lastname: Pessoa-Júnior
authors.affiliation: Departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco Dois IrmãosPE Brazil Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Dois Irmãos, PE, Brazil.
authors.forename: Ana L F
authors.initials: AL
authors.lastname: Porto
mesh_terms: D019818:Clavulanic Acid / Q000378:metabolism*; D021902:Enterobacter aerogenes / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000378:metabolism*; D008403:Mass Screening; D013302:Streptomyces / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D019818:Clavulanic Acid; D001618:beta-Lactamases
keywords: Streptomyces screening; clavulanic acid; growth kinetics; submerged culture; β-lactamases inhibitor