title: Application of CdO nanoparticle ionic liquid modified carbon paste electrode as a high sensitive biosensor for square wave voltammetric determination of NADH.
abstract: In this paper we report the synthesis and application of CdO nanoparticle (CdO/NPs) and 1,3-dipropylimidazolium bromide as high sensitive sensors for voltammetric determination of nicotinamide adenine dinucleotide (NADH) using carbon paste electrode. The cyclic voltammogram showed an irreversible oxidation peak at 0.68 V (vs. Ag/AgClsat), which corresponded to the oxidation of NADH. Compared to common carbon paste electrode, the electrochemical response was greatly improved for NADH electrooxidation at a surface of CdO/NP modified ionic liquid carbon paste electrode (IL/CdO/NP/CPE). The linear response range and detection limit were found to be 0.09-750 μmol L(-1) and 0.05 μmol L(-1), respectively. Result shows that other physiological species did not interfere in the determination of NADH at the surface of the proposed sensor in the optimum condition. The proposed sensor was successfully applied for the determination of NADH in tap water, urine and pharmaceutical serum samples.
authors.affiliation: Department of Physics, Sari Branch, Islamic Azad University, Sari, Iran. Electronic address: pahlavan1292000@yahoo.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Pahlavan
authors.affiliation: Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.karimi.maleh@gmail.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Karimi-Maleh
authors.affiliation: Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Karimi
authors.affiliation: Department of Biotechnology, Faculty of Chemical engineering, Mazandaran University of Science & Technology (MUST), Babol, Iran.
authors.forename: Mohsen Aboukazempour
authors.initials: MA
authors.lastname: Amiri
authors.affiliation: Department of Physics, Mazandaran Science and Research Branch, Mazandaran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khoshnama
authors.affiliation: Department of Solid State Physics, University of Mazandaran, Babolsar, Iran.
authors.forename: Mandana Roodbari
authors.initials: MR
authors.lastname: Shahmiri
authors.affiliation: Department of Chemistry, Majlesi Branch, Islamic Azad University, Isfahan, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Keyvanfard
mesh_terms: D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D052578:Ionic Liquids / Q000737:chemistry*; D009243:NAD / Q000032:analysis* / Q000097:blood / Q000652:urine; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; D052578:Ionic Liquids; D010087:Oxides; C029663:cadmium oxide; D009243:NAD; D002244:Carbon
keywords: CdO nanoparticles; Electrochemical impedance spectroscopy; Ionic liquid; NADH; Voltammetry