title: Active curcumin nanoparticles formed from a volatile microemulsion template.
abstract: We report on biological performance of organic nanoparticles formed by a simple method based on rapid solvent removal from a volatile microemulsion. The particular focus of the study was on testing the suitability of the method for substances soluble in partially water-miscible organic solvents as well as on evaluating the therapeutic activity of the resultant nanoparticles. Curcumin was employed as a model for hydrophobic drug, and, as it is soluble in water-miscible organic solvents, it was successfully incorporated into a new cyclopentanone-water microemulsion system. During rapid solvent removal by spray-drying, the nanometric droplets of the microemulsion were converted into nanoparticles containing amorphous curcumin with the average size of 20.2±3.4 nm, having ζ potential of -36.2 ±1.8 mV. These nanoparticles were dispersible in water and retained the high loading of the active substance. The therapeutic activity of the resulting nanoparticles was demonstrated in a pancreatic cancer cell line Panc-1. The effective concentration for reducing the metabolic activity was found to be 11.5 μM for nanoparticles compared with 19.5 μM for free curcumin.
authors.affiliation: Hebrew University of Jerusalem, Casali Institute of Applied Chemistry, Institute of Chemistry and Center for Nanoscience and Nanotechnology, 91904 Jerusalem, Israel. Tel +972-2-6584967, Fax +972-2-6584350.
authors.forename: K
authors.initials: K
authors.lastname: Margulis
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA.
authors.forename: S
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA ; College of Engineering, Swansea University, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Ware
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA ; College of Engineering, Swansea University, UK.
authors.forename: H D
authors.initials: HD
authors.lastname: Summers
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, TX, USA.
authors.forename: B
authors.initials: B
authors.lastname: Godin
authors.affiliation: Hebrew University of Jerusalem, Casali Institute of Applied Chemistry, Institute of Chemistry and Center for Nanoscience and Nanotechnology, 91904 Jerusalem, Israel. Tel +972-2-6584967, Fax +972-2-6584350.
authors.forename: S
authors.initials: S
authors.lastname: Magdassi