title: Tachycardia pacing induces myocardial neovascularization and mobilizes circulating endothelial progenitor cells partly via SDF-1 pathway in canines.
abstract: Neovascularization plays pivotal role in ischemic heart failure; however, it is unclear in non-ischemic heart failure. Non-ischemic heart failure was induced by chronic rapid right ventricular pacing at 200 beats/min, respectively, for 3 and 6 weeks in 12 dogs. Sham-operation was performed in another 6 dogs as control. Three-week tachycardia pacing could induce mild/moderate heart failure and 6-week pacing could induce severe heart failure. Pan-microvessel density (MVD) was assessed by CD31 and neovascularization density was assessed by CD105. Mean CD31-MVD and CD105-MVD were significantly increased after 3-week pacing. However, CD105-MVD was significantly decreased by 80 % in 6-week pacing group compared with 3-week pacing group, whereas CD31-MVD was only decreased slightly (15 %; P < 0.05). Myocardial proangiogenic factor stromal cell-derived factor 1 (SDF-1), hypoxia-inducible factors 1α (HIF-1α, a transcription factor which could regulate SDF-1 expression), serum SDF-1 levels and circulating EPC mobilization were greatly elevated after 3-week pacing but nearly returned to baseline level after 6-week pacing, which were in accordance with the changes of neovascularization levels assessed by CD105. Angiogenesis and migrating ability of EPCs were enhanced after stimulation of SDF-1, which could be abolished by pretreatment with SDF-1 receptor antagonist AMD3100. In addition, angiogenesis and migrating functions of EPCs were significantly enhanced by the serum from 3-week pacing dogs, but had much weaker response to the serum from 6-week pacing dogs. In conclusion, tachycardia pacing-induced non-ischemic heart failure, promoted myocardial neovascularization and mobilized circulating EPCs, which might be mediated partly through SDF-1 pathway.
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Mai
authors.affiliation: Department of Anesthesiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: Intelligent Computing Research Center, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, China.
authors.forename: Buzhou
authors.initials: B
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China.
authors.forename: YongQing
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China.
authors.forename: NianSang
authors.initials: N
authors.lastname: Luo
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China.
authors.forename: WoLiang
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Intelligent Computing Research Center, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, China.
authors.forename: XiaoLong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Intelligent Computing Research Center, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, 518055, China.
authors.forename: Qingcai
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China. dr_wjf@hotmail.com.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China. dr_wjf@hotmail.com.
authors.forename: JingFeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, 510120, China. tjcyx1995@163.com.|Guangdong Province Key Laboratory of Arrhythmia and Electrophysiology, Guangzhou, 510120, China. tjcyx1995@163.com.
authors.forename: YangXin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002304:Cardiac Pacing, Artificial; D002465:Cell Movement; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000378:metabolism*; D003331:Coronary Vessels / Q000378:metabolism* / Q000503:physiopathology; D004195:Disease Models, Animal; D004285:Dogs; D066026:Endothelial Progenitor Cells / Q000378:metabolism*; D006333:Heart Failure / Q000209:etiology* / Q000378:metabolism / Q000503:physiopathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D015398:Signal Transduction; D017180:Tachycardia, Ventricular / Q000150:complications*; D013997:Time Factors
chemical_list: D054377:Chemokine CXCL12; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Endothelial progenitor cells; Neovascularization; Non-ischemic heart failure; Tachycardia pacing