title: Fasciola hepatica in some Buffaloes and cattle by PCR and microscopy.
abstract: Fasciolosis is the burning problem of the livestock rearing community having huge morbidity, mortality, and economic losses to livestock industries in our country Pakistan. The faecal and liver biopsy samplings were examined by polymerase chain reaction (PCR) and microscopy technique during the entire study. A total of 307 samples including 149 samples from Karak and 158 samples from Kohat abattoirs were examined by PCR method and overall prevalence of fasciolosis was 5.86% (18/307), amongst theses 8.05% (12/149) in liver biopsy and 3.79% (6/158) in feacal samples of cattle and Buffaloes were recorded. Similarly the microscopy based detection was 3.58% (11/307) including 4.61% (7/149) in liver biopsy and 2.5% (4/158) in faecal samples accordingly. Furthermore the areawise prevalence of fasciolosis in abattoirs by PCR method was found to be 7.59% (12/158) in Kohat and 4.02% (6/149) in Karak. A 618 pb DNA was amplified in 2% agarose gel electrophoreses. It is concluded from the study that prevalence of fasciolosis was higher in abattoir of district Kohat and PCR was a more sensitive method of diagnosis than microscopy.
authors.affiliation: College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, Pakistan.
authors.forename: Sultan
authors.initials: S
authors.lastname: Ayaz
authors.affiliation: Department of Chemistry, Government College Ara Khel, FR Kohat, Khyber Pakhtunkhwa 26000, Pakistan.
authors.forename: Riaz
authors.initials: R
authors.lastname: Ullah
authors.affiliation: Riyadh Community College, King Saud University, Riyadh 11437, Saudi Arabia.
authors.forename: Naser M
authors.initials: NM
authors.lastname: AbdEl-Salam
authors.affiliation: Department of Zoology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, Pakistan.
authors.forename: Sumiara
authors.initials: S
authors.lastname: Shams
authors.affiliation: Department of Zoology, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa 23200, Pakistan.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Niaz
mesh_terms: D000003:Abattoirs; D000818:Animals; D002020:Buffaloes / Q000469:parasitology*; D002417:Cattle / Q000469:parasitology*; D002418:Cattle Diseases / Q000469:parasitology*; D005210:Fasciola hepatica / Q000502:physiology*; D005211:Fascioliasis / Q000175:diagnosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D008853:Microscopy / Q000379:methods*; D010154:Pakistan / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence