title: Involvement of oxidative stress in SAMP10 mice with age-related neurodegeneration.
abstract: Age-related changes in the brain tissue are reflected in many aspects. We sought to determine the morphology, Nissl bodies, behavioral appearance and oxidative stress in the brain using SAMP10 mice, a substrain of the senescence-accelerated mouse. SAMP10 mice groups divided by different ages (3, 5, 8 and 14 months) were compared with those of control groups with the above corresponding ages. Cortical thickness, Nissl bodies, behavioral appearance and oxidative stress were evaluated through image software, thionine staining, step-down test and colorimetry, respectively. The weight and cortical thickness of the brain in SAMP10 mice significantly reduced from 8 months of age. The results showed that the number of Nissl bodies decreased or Nissl bodies shrank with dark staining in histology. The same result appeared in a step-down test. As the SAMP10 mice grew older, the oxidative stress-related markers superoxide dismutase decreased and malondialdehyde increased after 8 months. Glutathione peroxidase activities showed no age-related changes. The changes of brain morphology and productions of oxidative stress in the brain tissue might contribute to the behavioral abnormality. Deceleration of age-related production of oxidative stress might be expected to be a potent strategy for anti-aging interventions.
authors.affiliation: Institute of Basic Theory, China Academy of Chinese Medical Sciences, 16 Dong Zhi Men Nei Nan Xiao Jie, Beijing, 100700, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Hongtao
authors.initials: H
authors.lastname: Lei
authors.forename: Jincai
authors.initials: J
authors.lastname: Hou
authors.forename: Jianxun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics*; D000704:Analysis of Variance; D000818:Animals; D001362:Avoidance Learning; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D019636:Neurodegenerative Diseases / Q000235:genetics* / Q000473:pathology / Q000503:physiopathology*; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase