title: Enhanced Schwann cell attachment and alignment using one-pot "dual click" GRGDS and YIGSR derivatized nanofibers.
abstract: Using metal-free click chemistry and oxime condensation methodologies, GRGDS and YIGSR peptides were coupled to random and aligned degradable nanofiber networks postelectrospinning in a one-pot reaction. The bound peptides are bioactive, as demonstrated by Schwann cell attachment and proliferation, and the inclusion of YIGSR with GRGDS alters the expression of the receptor for YIGSR. Additionally, aligned nanofibers act as a potential guidance cue by increasing the aspect ratio and aligning the actin filaments, which suggest that peptide-functionalized scaffolds would be useful to direct SCs for peripheral nerve regeneration.
authors.affiliation: Departments of ‡Polymer Science and §Biomedical Engineering, The University of Akron , Akron, Ohio 44325, United States.
authors.forename: Jukuan
authors.initials: J
authors.lastname: Zheng
authors.forename: Dimitria
authors.initials: D
authors.lastname: Kontoveros
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.forename: Geng
authors.initials: G
authors.lastname: Hua
authors.forename: Darrell H
authors.initials: DH
authors.lastname: Reneker
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Becker
authors.forename: Rebecca K
authors.initials: RK
authors.lastname: Willits
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D057930:Click Chemistry; D057139:Nanofibers / Q000737:chemistry*; D009842:Oligopeptides / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D009842:Oligopeptides; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; C049554:glycyl-arginyl-glycyl-aspartyl-serine