title: Morphology and mycelial growth rate of Pleurotus spp. strains from the Mexican mixtec region.
abstract: Two native Pleurotus spp. strains (white LB-050 and pale pink LB-051) were isolated from rotten tree trunks of cazahuate (Ipomoea murucoides) from the Mexican Mixtec Region. Both strains were chemically dedikaryotized to obtain their symmetrical monokaryotic components (neohaplonts). This was achieved employing homogenization time periods from 60 to 65 s, and 3 day incubation at 28 °C in a peptone-glucose solution (PGS). Pairing of compatible neohaplonts resulted in 56 hybrid strains which were classified into the four following hybrid types: (R(1-n)xB(1-n), R(1-n)xB(2-1), R(2-n)xB(1-n) and R(2-n)xB(2-1)). The mycelial growth of Pleurotus spp. monokaryotic and dikaryotic strains showed differences in texture (cottony or floccose), growth (scarce, regular or abundant), density (high, regular or low), and pigmentation (off-white, white or pale pink). To determine the rate and the amount of mycelium growth in malt extract agar at 28 °C, the diameter of the colony was measured every 24 h until the Petri dish was completely colonized. A linear model had the best fit to the mycelial growth kinetics. A direct relationship between mycelial morphology and growth rate was observed. Cottony mycelium presented significantly higher growth rates (p < 0.01) in comparison with floccose mycelium. Thus, mycelial morphology can be used as criterion to select which pairs must be used for optimizing compatible-mating studies. Hybrids resulting from cottony neohaplonts maintained the characteristically high growth rates of their parental strains with the hybrid R(1-n)xB(1-n) being faster than the latter.
authors.affiliation: Instituto de Agroindustrias Universidad Tecnológica de la Mixteca Huajuapan de LeónOaxaca México Instituto de Agroindustrias, Universidad Tecnológica de la Mixteca, Huajuapan de León, Oaxaca, México.
authors.forename: P C
authors.initials: PC
authors.lastname: Guadarrama-Mendoza
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico Nacional Col. TicomanMéxico D.F. México Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Col. Ticoman, México D.F., México.
authors.forename: G Valencia
authors.initials: GV
authors.lastname: del Toro
authors.affiliation: Facultad de Química Universidad Nacional Autónoma de México México D.F. México. Facultad de Química, Universidad Nacional Autónoma de México, México D.F., México.
authors.forename: R
authors.initials: R
authors.lastname: Ramírez-Carrillo
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico Nacional Col. TicomanMéxico D.F. México Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Col. Ticoman, México D.F., México.
authors.forename: F
authors.initials: F
authors.lastname: Robles-Martínez
authors.affiliation: Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico Nacional Col. TicomanMéxico D.F. México Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Col. Ticoman, México D.F., México.
authors.forename: J
authors.initials: J
authors.lastname: Yáñez-Fernández
authors.affiliation: Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Tlalnepantla, Estado de México México Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla, Estado de México, México.
authors.forename: M E
authors.initials: ME
authors.lastname: Garín-Aguilar
authors.affiliation: Instituto de Agroindustrias Universidad Tecnológica de la Mixteca Huajuapan de LeónOaxaca México Instituto de Agroindustrias, Universidad Tecnológica de la Mixteca, Huajuapan de León, Oaxaca, México.
authors.forename: C G
authors.initials: CG
authors.lastname: Hernández
authors.affiliation: Instituto de Agroindustrias Universidad Tecnológica de la Mixteca Huajuapan de LeónOaxaca México Instituto de Agroindustrias, Universidad Tecnológica de la Mixteca, Huajuapan de León, Oaxaca, México.
authors.forename: G
authors.initials: G
authors.lastname: Bravo-Villa
mesh_terms: D003433:Crosses, Genetic; D003470:Culture Media / Q000737:chemistry; D008800:Mexico; D025282:Mycelium / Q000254:growth & development*; D010860:Pigments, Biological / Q000378:metabolism; D020076:Pleurotus / Q000254:growth & development* / Q000302:isolation & purification; D013696:Temperature; D014197:Trees / Q000382:microbiology
chemical_list: D003470:Culture Media; D010860:Pigments, Biological
keywords: Pleurotus; hybrid strains; monokaryotization; mycelial growth; mycelial morphology