title: Computational protein design methods for synthetic biology.
abstract: Computational protein design, a process that searches for mutants with desired improved properties, plays a central role in the conception of many synthetic biology devices including biosensors, bioproduction, or regulation circuits. To that end, a rational workflow for computational protein design is described here consisting of (a) searching in the sequence, structure or chemical spaces for the desired function and associated protein templates; (b) finding the list of potential hot regions to mutate in the parent proteins; and (c) performing in silico screening of mutants with predicted improved properties.
authors.affiliation: Research Program on Biomedical Informatics (GRIB), Department of Experimental and Health Sciences, Universitat Pompeu Fabra, IMIM (Hospital del Mar Medical Research Institute), Dr. Aiguader, 88 E-8003, Barcelona, Spain, pablo.carbonell@issb.genopole.fr.
authors.forename: Pablo
authors.initials: P
authors.lastname: Carbonell
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Trosset
mesh_terms: D019295:Computational Biology / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D058615:Synthetic Biology / Q000379:methods*
chemical_list: D011506:Proteins