title: Controlling the release of bFGF from silk fibroin membrane.
abstract: Since neurotrophic factor is easy to degrade and aggregate, it usually has a short half-life in vitro. To overcome this shortage, neurotrophic factor has been combined with the silk fibroin (SF) membrane to realize less degradation, optimal loading efficiency, sustained release, and good adsorption. By optimizing its binding conditions, main parameters were investigated and its optimal loading efficiency was obtained. bFGF was combined to SF membrane by layer by layer (LbL) static adsorption technique. The natural and nontoxic chondroitin sulfate (CS) was used as a crosslinking agent. Optimization was carried out in three aspects: the concentration of bFGF, the concentration of CS, and the reaction time. This experiment provides a better environment for the growth of cells and offers a new kind material of absorbing neurotrophic factor to meet increasing demand for biological materials.
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Ya Wei
authors.initials: YW
authors.lastname: Ji
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Ya Hong
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: College of Chemistry and Chemical Engineering, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Ya Ling
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Lu Zhong
authors.initials: LZ
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, Jiangsu, China; The Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong University, Nantong 226001, Jiangsu, China.
authors.forename: Yu Min
authors.initials: YM
authors.lastname: Yang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D016716:PC12 Cells; D051381:Rats
chemical_list: D016222:Fibroblast Growth Factor 2; D005349:Fibroins