title: Polyethyleneimine-iron phosphate nanocomposite as a promising adsorbent for the isolation of DNA.
abstract: A polyethyleneimine (PEI)-iron phosphate (FePO4) nanocomposite is prepared by immobilization of PEI onto the surface of FePO4 nanoparticles via electrostatic interaction. The obtained PEI-FePO4 nanocomposites are spherical with a size centered in ca. 100 nm. They provide a novel adsorbent for the solid-phase extraction of DNA from complex sample matrices. At pH 4, 50 μg mL(-1) of DNA (salmon sperm DNA sodium salt) in 1.0 mL aqueous solution are quantitatively adsorbed (100%) by 2mg of the PEI-FePO4 nanocomposites, and meanwhile the coexisting albumin at a same concentration level is not retained, demonstrating the favorable selectivity of the nanocomposites to DNA against proteins. The adsorption behaviors of DNA onto the PEI-FePO4 nanocomposites fit Langmuir model, corresponding to an adsorption capacity of 61.88 mg g(-1). The adsorbed DNA could be readily recovered by using a 0.04 mol L(-1) Britton-Robinson (BR) buffer at pH 10, resulting in a recovery of 85%. The nanocomposites have been further used for the isolation of DNA from a series of real sample matrices, including synthetic λ-DNA sample, human whole blood and Escherichia coli cell lysate. The extraction efficiency and the purity of the recovered DNA are at least comparable to those achieved by using the reported sorbent materials or commercial kits. In addition, the DNAs isolated from human whole blood and E. coli cell lysate are of high quality, which have been further demonstrated by using them as templates for successful PCR amplifications.
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Hu
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Hu
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Shen
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: chenxuwei@mail.neu.edu.cn.
authors.forename: Xu-Wei
authors.initials: XW
authors.lastname: Chen
authors.affiliation: Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang 110819, China. Electronic address: jianhuajrz@mail.neu.edu.cn.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000327:Adsorption; D000818:Animals; D001773:Blood Cells / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry; D005296:Ferrous Compounds / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D053761:Nanocomposites / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012474:Salmon / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods*; D013094:Spermatozoa / Q000737:chemistry; D055672:Static Electricity
chemical_list: D005296:Ferrous Compounds; D010710:Phosphates; D011094:Polyethyleneimine; D004247:DNA; C518753:ferrous phosphate
keywords: DNA; Iron phosphate; Nanocomposite; Polyethyleneimine; Solid-phase extraction