title: [Interactions of [NSI+] determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae].
abstract: Previously we characterized [NSI+] determinant, that possesses the features of a yeast prion. This determinant causes the nonsense suppression in strains that bear different N-substituted variants of Sup35p, which is a translation release factor eRF3. As a result of the genomic screen, we identified VTS1, the overexpression of which is a phenotypic copy of [NSI+]. Here, we analyzed the influence of SUP35 and VTS1 on [NSI+]. We demonstrated nonsense suppression in the [NSI+] strains, which appears when SUP35 expression was decreased or against a background of general defects in the fidelity of translation termination. [NSI+] has also been shown to increase VTS1 mRNA amounts. These findings facilitate the insight into the mechanisms of nonsense suppression in the [NSI+] strains and narrow the range of candidates for [NSI+] determinant.
authors.forename: A A
authors.initials: AA
authors.lastname: Nizhnikov
authors.forename: A M
authors.initials: AM
authors.lastname: Kondrashkina
authors.forename: A P
authors.initials: AP
authors.lastname: Galkin
mesh_terms: D015966:Gene Expression Regulation, Fungal; D010454:Peptide Termination Factors / Q000235:genetics* / Q000378:metabolism; D011328:Prions; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D010454:Peptide Termination Factors; D011328:Prions; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C508663:Vts1 protein, S cerevisiae