title: Orbitrap™ monostage MS versus hybrid linear ion trap MS: application to multi-allergen screening in wine.
abstract: Food allergen research has made giant steps in the last years thanks to the features offered by the latest technology of mass analyzers placed on the market allowing multiplex sensitive detection of proteins. Potentials and features of two mass analyzers namely a linear ion trap capable of performing a data dependent or selected reaction monitoring analysis and an Orbitrap(TM) stand-alone MS enabling a broadband fragmentation without mass selection at highest mass resolving power are herein described and applied to the multiplex screening of allergens in a type of wine chosen as a reference matrix. Quantitative and confirmative capabilities of both platforms were assessed on the specific case study, the multiple detection of egg and milk -related proteins, typically employed in white wines as fining agents. Commercial bioinformatic tools used for a quick allergen identification will be also discussed.
authors.affiliation: Institute of Sciences of Food Production, National Research Council of Italy (ISPA-CNR), Via Amendola 122/O, 70126, Bari, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Pilolli
authors.forename: Elisabetta
authors.initials: E
authors.lastname: De Angelis
authors.forename: Michal
authors.initials: M
authors.lastname: Godula
authors.forename: Angelo
authors.initials: A
authors.lastname: Visconti
authors.forename: Linda
authors.initials: L
authors.lastname: Monaci
keywords: OrbitrapTM; high-resolution MS; linear ion trap; mass spectrometry; multi-allergen screening; wine