title: Detection of immunoglobulin IGH gene rearrangements on formalin-fixed, paraffin embedded tissue in lymphoid malignancies.
abstract: Human lymphomas are aggressive malignant diseases, which can be categorized based on their B and T cell lineage. B-cell lymphomas form around 90% of the total lymphoma cases, the remnants of malignancies arise from the T cell branch. Lymphomas are mostly characterized as clonal proliferations of specific tumor cells. The detection of malignant lymphomas are extensively investigated by their morphological features, immunohistochemistry and flowcytometric immunophenotyping, but in some of cases remained unknown. The BIOMED-2 protocols were used to determine the clonality of IGH gene rearrangements in patients with lymphoma. PCR amplification was performed on FFPE of 50 patients with B-cell lymphoma, which consisted of 11 cases with HLs, 25 cases of B-NHLs and 14 cases of B-LPD (lymphoproliferative disorders) that diagnosed as unclassifiable lymphoma. The rate of positive clonality was detected in 96% (24/25) of B-NHLs, whereas in 4% (1/25) of cases clonality was showed in a polyclonal pattern. In B-HLs, 82% (9/11) of cases showed clonality and 18% (2/11) of the cases showed polyclonality. The rate of positive clonality observed in 64.3% (9/14) of cases with B-LPD and 35.7% (5/14) of cases clonality was not detected in any of immunoglobulin gene family (FR1, FR2, FR3). In groups with DLBCL, clonality was detected in 95% (19/20) of the cases. In patients diagnosed with FL and MALTs 100% cases showed clonality for complete IGH. Our study revealed that EuroClonality BIOMED-2 protocols could be considered as a valuable and reliable method for clonality detection, especially in IGH analysis.
authors.affiliation: Tabriz University of Medical Sciences Biotechnology Research Center and Department of Medical Genetics Tabriz Iran.
authors.forename: G
authors.initials: G
authors.lastname: Moharrami
authors.affiliation: Islamic Azad University Department of Biology, Science and Research Branch Tehran Iran.
authors.forename: S
authors.initials: S
authors.lastname: Ghorbian
authors.affiliation: University of Alberta Department of Medical Genetics, Faculty of Medicine and Dentistry Alberta Canada.
authors.forename: M
authors.initials: M
authors.lastname: Seifi
authors.affiliation: Tehran University of Medical Sciences Department of Medical Genetics, School of Medicine Tehran Iran.
authors.forename: M A
authors.initials: MA
authors.lastname: Estiar
authors.affiliation: Tabriz University of Medical Sciences Department of Pathology, School of Medicine Tabriz Iran.
authors.forename: A
authors.initials: A
authors.lastname: Fakhrjoo
authors.affiliation: University of Liverpool Faculty of Medicine Liverpool United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Sakhinia
authors.affiliation: Tabriz University of Medical Sciences Biotechnology Research Center Tabriz Iran esakhinia&commat;yahoo.co.uk.
authors.forename: E
authors.initials: E
authors.lastname: Sakhinia
mesh_terms: D005260:Female; D005557:Formaldehyde / Q000737:chemistry; D015321:Gene Rearrangement; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics*; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D016707:Tissue Fixation
chemical_list: D007136:Immunoglobulins; D015514:Oncogene Proteins, Fusion; D005557:Formaldehyde