title: A novel hemp seed meal protein hydrolysate reduces oxidative stress factors in spontaneously hypertensive rats.
abstract: This report shows the antioxidant effects of a hemp seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted hemp seed meal was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which was incorporated into rat feed as a source of antioxidant peptides. Young (8-week old) SHRs were divided into three groups (8 rats/group) and fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half of the rats were sacrificed for blood collection. After a 4-week washout period, the remaining 20-week old SHRs were fed for an additional four weeks and sacrificed for blood collection. Plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD), catalase (CAT) and total peroxides (TPx) levels were determined. Results showed that plasma TAC, CAT and SOD levels decreased in the older 20-week old SHRs when compared to the young SHRs. The presence of HMH in the diets led to significant (p < 0.05) increases in plasma SOD and CAT levels in both young and adult SHR groups; these increases were accompanied by decreases in TPx levels. The results suggest that HMH contained antioxidant peptides that reduced the rate of lipid peroxidation in SHRs with enhanced antioxidant enzyme levels and total antioxidant capacity.
authors.affiliation: Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg MB R3T 2N2, Canada. umgirgia@myumanitoba.ca
authors.forename: Abraham T
authors.initials: AT
authors.lastname: Girgih
authors.forename: Adeola M
authors.initials: AM
authors.lastname: Alashi
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Sunday A
authors.initials: SA
authors.lastname: Malomo
authors.forename: Pema
authors.initials: P
authors.lastname: Raj
authors.forename: Thomas
authors.initials: T
authors.lastname: Netticadan
authors.forename: Rotimi E
authors.initials: RE
authors.lastname: Aluko
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002188:Cannabis; D002374:Catalase / Q000097:blood; D004044:Dietary Proteins / Q000008:administration & dosage*; D004195:Disease Models, Animal; D006973:Hypertension / Q000097:blood / Q000178:diet therapy* / Q000503:physiopathology; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D010545:Peroxides / Q000097:blood; D010940:Plant Proteins / Q000008:administration & dosage*; D011492:Protein Hydrolysates / Q000008:administration & dosage*; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012639:Seeds; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors
chemical_list: D015415:Biomarkers; D004044:Dietary Proteins; D010545:Peroxides; D010940:Plant Proteins; D011492:Protein Hydrolysates; D002374:Catalase; D013482:Superoxide Dismutase