title: A fluorescent polymer dots positive readout fluorescent quenching lateral flow sensor for ractopamine rapid detection.
abstract: A fluorescent polymer dots positive readout and sensitive lateral flow assay (LFA) based on fluorescent quenching has been developed to detect ractopamine (Rac), a chemical residue in food, harmful to human health. Compared with traditional LFA strips, these fluorescent quenching LFA (FQLFA) strips provide a positive correlation method that allows users to obtain results from a weak fluorescent signal. The immunoassay strip scheme is based on the fact that fluorescent polymer dots (FPDs) in close proximity to gold nanoparticles (AuNPs) represent a strong fluorescent quenching. We show that the FQLFA strips can be used as a source to quantitatively analyze Rac in phosphate buffers (PB), swine urine and muscle tissue samples. The lowest detection limitation of the FQLFA was 0.16 ng mL(-1). Our results indicated that this novel scheme was more suitable for rapid detection of small molecules.
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: Cong Ying
authors.initials: CY
authors.lastname: Shi
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Deng
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: Jia Jie
authors.initials: JJ
authors.lastname: Liang
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: Ke Nan
authors.initials: KN
authors.lastname: Zhou
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China.
authors.forename: Qiang Qiang
authors.initials: QQ
authors.lastname: Fu
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, PR China. Electronic address: tyjaq7926@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry*; D010627:Phenethylamines / Q000737:chemistry*; D013552:Swine
chemical_list: D005456:Fluorescent Dyes; D010627:Phenethylamines; C060897:ractopamine
keywords: Fluorescent quenching; Lateral flow sensors; Positive readout; Ractopamine