title: MWCNTs enhance hBMSCs spreading but delay their proliferation in the direction of differentiation acceleration.
abstract: Investigating the ability of films of pristine multiwalled nanotubes (MWCNTs) to influence human mesenchymal stem cells' proliferation, morphology, and differentiation into osteoblasts, we concluded to the following: A. MWCNTs delay the proliferation of hBMS cells but increase their differentiation. The enhancement of the differentiation markers could be a result of decreased proliferation and maturation of the extracellular matrix B. Cell spread on MWCNTs toward a polygonal shape with many thin filopodia to attach to the surfaces. Spreading may be critical in supporting osteogenic differentiation in pre-osteoblastic progenitors, being related with cytoskeletal tension. C. hBMS cells prefer MWCNTs than tissue plastic to attach and grow, being non-toxic to these cells. MWCNTs can be regarded as osteoinductive biomaterial topographies for bone regenerative engineering.
authors.affiliation: a Department of Mechanical Engineering & Aeronautics ; University of Patras ; Rion , Greece.
authors.forename: Despina D
authors.initials: DD
authors.lastname: Deligianni
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D043942:Nanotubes; D010006:Osteoblasts / Q000166:cytology*