title: In vitro analysis of cleavage and polyadenylation in Arabidopsis.
abstract: In eukaryotes, pre-messenger RNA (pre-mRNA) cleavage and polyadenylation is one of the necessary processing steps that produce a mature and functional mRNA. Regulation on pre-mRNA cleavage and polyadenylation affects other processes such as mRNA translocation, stability, and translation. The process of pre-mRNA cleavage and polyadenylation, and its relationship with RNA splicing and translation, have been extensively studied due to its importance in vivo. A successful in vitro system has provided enormous amount of information to the study of cleavage and polyadenylation in the mammalian and yeast systems. Here, we describe an in vitro pre-mRNA cleavage system that faithfully cleaves pre-mRNA substrate using Arabidopsis cell/tissue cultures.
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China, hzhao@njau.edu.cn.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhao
authors.forename: Qingshun Quinn
authors.initials: QQ
authors.lastname: Li
mesh_terms: D017360:Arabidopsis; D002457:Cell Extracts; D026723:Polyadenylation; D059367:RNA Cleavage; D012322:RNA Precursors / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism*
chemical_list: D002457:Cell Extracts; D012322:RNA Precursors; D018749:RNA, Plant