title: The benefit of bone marrow concentrate in addition to a glass-reinforced hydroxyapatite for bone regeneration: An in vivo ovine study.
abstract: This study evaluates the ability of a Glass Reinforced Hydroxyapatite Composite (GRHC), in a new microporous pellet formulation with autologous bone marrow concentrate (BMC), to enhance bone regeneration and new bone formation. Ninety non-critical sized bone defects were created in the femurs of nine Merino breed sheep and randomly left unfilled (group A), filled with GRHC pellets alone (group B) or filled with GRHC pellets combined with BMC (group C). The sheep were sacrificed at 3 weeks (three sheep), 6 weeks (three sheep) and 12 weeks (three sheep) and histological analysis (Light Microscopy-LM), scanning electron microscopy (SEM) and histomorphometric analysis (HM) were performed. At 3, 6, and 12 weeks, HM revealed an average percentage of new bone of 48, 72, 83%; 25, 73, 80%, and 16, 38, 78% for Groups C, B and A respectively (significantly different only at 3 weeks p < 0.05). LM and SEM evaluation revealed earlier formation of well-organized mature lamellar bone in Group C. This study demonstrates that the addition of a bone marrow concentrate to a glass reinforced hydroxyapatite composite in a pellet formulation promotes early bone healing. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1176-1182, 2017.
authors.affiliation: Faculty of Medicine, University of Porto, Alameda Hernani Monteiro, 4200-319, Porto, Portugal.
authors.forename: Joao
authors.initials: J
authors.lastname: Torres
authors.affiliation: Faculty of Medicine, University of Porto, Alameda Hernani Monteiro, 4200-319, Porto, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Gutierres
authors.affiliation: Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n228, 4050-313, Porto, Portugal.
authors.forename: Luis
authors.initials: L
authors.lastname: Atayde
authors.affiliation: Departamento de Clinicas Veterinarias, Instituto de Ciencias Biomedicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, n228, 4050-313, Porto, Portugal.
authors.forename: Paulo
authors.initials: P
authors.lastname: Cortez
authors.affiliation: CEMUC, Faculdade de Engenharia, Universidade do Porto, Rua Doutor Roberto Frias, 4200-465, Porto, Portugal.
authors.forename: M Ascenção
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: CEMUC, Faculdade de Engenharia, Universidade do Porto, Rua Doutor Roberto Frias, 4200-465, Porto, Portugal.
authors.forename: J Domingos
authors.initials: JD
authors.lastname: Santos
authors.affiliation: Faculty of Medicine, University of Porto, Alameda Hernani Monteiro, 4200-319, Porto, Portugal.
authors.forename: Abel T
authors.initials: AT
authors.lastname: Cabral
authors.affiliation: Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, 3471 Fifth Avenue, Kaufman building suite 1011, Pittsburgh, Pennsylvania, 15213.
authors.forename: Carola F
authors.initials: CF
authors.lastname: van Eck
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration; D005269:Femur / Q000000981:diagnostic imaging / Q000648:ultrastructure; D006882:Hydroxyapatites / Q000627:therapeutic use*; D008855:Microscopy, Electron, Scanning; D011859:Radiography; D011897:Random Allocation; D012756:Sheep; D017640:Silicates / Q000627:therapeutic use*
chemical_list: D006882:Hydroxyapatites; D017640:Silicates
keywords: bone healing; bone marrow; bone substitute