title: Naturally occurring N(6)-substituted adenosines (cytokinin ribosides) are in vitro inhibitors of platelet aggregation: an in silico evaluation of their interaction with the P2Y(12) receptor.
abstract: A few naturally occurring N(6)-substituted adenosine derivatives (cytokinin ribosides) were investigated as inhibitors of platelet aggregation induced in vitro by collagen and their activity range was demonstrated (IC50: 6.77-141 μM). A docking study suggests that anti-aggregation activity of these compounds could involve an interaction with the P2Y12 receptor binding site.
authors.affiliation: Department of Pharmaceutical Science, Università degli Studi, Via Celoria 2, 20100 Milano, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Vistoli
authors.affiliation: Department of Health Sciences, Università degli Studi, Via A. Di Rudinì 8, 20142 Milano, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Brizzolari
authors.affiliation: Department of Health Sciences, Università degli Studi, Via A. Di Rudinì 8, 20142 Milano, Italy; S. Paolo Hospital, Via A. Di Rudinì 8, 20142 Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Faioni
authors.affiliation: S. Paolo Hospital, Via A. Di Rudinì 8, 20142 Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Razzari
authors.affiliation: Department of Health Sciences, Università degli Studi, Via A. Di Rudinì 8, 20142 Milano, Italy. Electronic address: enzo.santaniello@unimi.it.
authors.forename: Enzo
authors.initials: E
authors.lastname: Santaniello
mesh_terms: D000241:Adenosine / Q000737:chemistry / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism*; D003583:Cytokinins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D010937:Plant Growth Regulators / Q000737:chemistry / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D058906:Purinergic P1 Receptor Agonists / Q000737:chemistry / Q000378:metabolism; D058925:Receptors, Purinergic P2Y12 / Q000378:metabolism*; D012263:Ribonucleosides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003583:Cytokinins; D010937:Plant Growth Regulators; D010975:Platelet Aggregation Inhibitors; D058906:Purinergic P1 Receptor Agonists; D058925:Receptors, Purinergic P2Y12; D012263:Ribonucleosides; D000244:Adenosine Diphosphate; D000241:Adenosine
keywords: Anti-aggregation activity; Cytokinin ribosides; Homology modeling; Molecular docking; P2Y(12) receptor; Platelets