title: Replication of obesity and diabetes-related SNP associations in individuals from Yucatán, México.
abstract: The prevalence of type 2 diabetes (T2D) is rising rapidly and in Mexicans is ~19%. T2D is affected by both environmental and genetic factors. Although specific genes have been implicated in T2D risk few of these findings are confirmed in studies of Mexican subjects. Our aim was to replicate associations of 39 single nucleotide polymorphisms (SNPs) from 10 genes with T2D-related phenotypes in a community-based Mexican cohort. Unrelated individuals (n = 259) living in southeastern Mexico were enrolled in the study based at the University of Yucatan School of Medicine in Merida. Phenotypes measured included anthropometric measurements, circulating levels of adipose tissue endocrine factors (leptin, adiponectin, pro-inflammatory cytokines), and insulin, glucose, and blood pressure. Association analyses were conducted by measured genotype analysis implemented in SOLAR, adapted for unrelated individuals. SNP Minor allele frequencies ranged from 2.2 to 48.6%. Nominal associations were found for CNR1, SLC30A8, GCK, and PCSK1 SNPs with systolic blood pressure, insulin and glucose, and for CNR1, SLC30A8, KCNJ11, and PCSK1 SNPs with adiponectin and leptin (p < 0.05). P-values greater than 0.0014 were considered significant. Association of SNPs rs10485170 of CNR1 and rs5215 of KCNJ11 with adiponectin and leptin, respectively, reached near significance (p = 0.002). Significant association (p = 0.001) was observed between plasma leptin and rs5219 of KCNJ11.
authors.affiliation: Facultad de Medicina, Merida, Universidad Autonoma de Yucatan Yucatan, Mexico.
authors.forename: Victor M
authors.initials: VM
authors.lastname: Hernandez-Escalante
authors.affiliation: Facultad de Salud Publica y Nutricion, Universidad Autonoma de Nuevo Leon Nuevo Leon, Monterrey, Mexico.
authors.forename: Edna J
authors.initials: EJ
authors.lastname: Nava-Gonzalez
authors.affiliation: Nutrition and UNC Nutrition Research Institute, University of North Carolina Chapel Hill, NC, USA.
authors.forename: V Saroja
authors.initials: VS
authors.lastname: Voruganti
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Jack W
authors.initials: JW
authors.lastname: Kent
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Karin
authors.initials: K
authors.lastname: Haack
authors.affiliation: Departamento de Investigación, Escuela de Ciencias de la Salud, Universidad Marista de Merida Merida, Yucatan, Mexico.
authors.forename: Hugo A
authors.initials: HA
authors.lastname: Laviada-Molina
authors.affiliation: Departamento de Investigación, Escuela de Ciencias de la Salud, Universidad Marista de Merida Merida, Yucatan, Mexico.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Molina-Segui
authors.affiliation: Facultdad de Enfermeria, Universidad Autonoma de Nuevo Leon Nuevo Leon, Monterrey, Mexico.
authors.forename: Esther C
authors.initials: EC
authors.lastname: Gallegos-Cabriales
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Lopez-Alvarenga
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Cole
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Marguerite J
authors.initials: MJ
authors.lastname: Mezzles
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Anthony G
authors.initials: AG
authors.lastname: Comuzzie
authors.affiliation: Department of Genetics, Texas Biomedical Research Institute San Antonio, TX, USA.
authors.forename: Raul A
authors.initials: RA
authors.lastname: Bastarrachea
keywords: Single Nucleotide Polymorphisms (SNP); association analysis; minor allele frequency; obesity; type 2 diabetes