title: Bacteria encapsulated in layered double hydroxides: towards an efficient bionanohybrid for pollutant degradation.
abstract: A soft chemical process was successfully used to immobilize Pseudomonas sp. strain ADP (ADP), a well-known atrazine (herbicide) degrading bacterium, within a Mg2Al-layered double hydroxide host matrix. This approach is based on a simple, quick and ecofriendly direct coprecipitation of metal salts in the presence of a colloidal suspension of bacteria in water. It must be stressed that by this process the mass ratio between inorganic and biological components was easily tuned ranging from 2 to 40. This ratio strongly influenced the biological activity of the bacteria towards atrazine degradation. The better results were obtained for ratios of 10 or lower, leading to an enhanced atrazine degradation rate and percentage compared to free cells. Moreover the biohybrid material maintained this biodegradative activity after four cycles of reutilization and 3 weeks storage at 4°C. The ADP@MgAl-LDH bionanohybrid materials were completely characterized by X-ray diffraction (XRD), FTIR spectroscopy, thermogravimetric analysis and scanning and transmission electronic microscopy (SEM and TEM) evidencing the successful immobilization of ADP within the inorganic matrix. This synthetic approach could be readily extended to other microbial whole-cell immobilization of interest for new developments in biotechnological systems.
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Matilte
authors.initials: M
authors.lastname: Halma
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Mousty
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Forano
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Martine
authors.initials: M
authors.lastname: Sancelme
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Besse-Hoggan
authors.affiliation: Clermont Université, Université Blaise Pascal, Institut de Chimie de Clermont-Ferrand, BP 10448, F-63000 Clermont-Ferrand, France; CNRS, UMR 6296, ICCF, F-63171 Aubiere, France. Electronic address: vanessa.prevot@univ-bpclermont.fr.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Prevot
mesh_terms: D001280:Atrazine / Q000737:chemistry / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002478:Cells, Cultured; D018914:Cells, Immobilized / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D006878:Hydroxides / Q000737:chemistry*; D050296:Microbial Viability; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D011549:Pseudomonas / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D006878:Hydroxides; D001280:Atrazine
keywords: Atrazine; Bacteria; Biodegradation; Bionanohybrid; Layered double hydroxides