title: Structural investigation of a uronic acid-containing polysaccharide from abalone by graded acid hydrolysis followed by PMP-HPLC-MSn and NMR analysis.
abstract: A new strategy was applied to elucidate the structure of a polysaccharide from abalone gonad (AGSP). It was hydrolyzed by 0.05 M, 0.2 M, 0.5 M, and 2.0 M TFA at 100 °C for 1 h, sequentially. Every hydrolysate was ultrafiltrated (3000 Da) to collect oligo- and monosaccharides, and the final retentate was further hydrolyzed with 2.0 M TFA at 110 °C and 121 °C for 2 h, respectively. 1-Phenyl-3-methyl-5-pyrazolone (PMP) derivatization followed by HPLC-MSn analysis was applied to detect the sugar residues in these hydrolysates, which allowed proposing their location in the polysaccharide structure. The retentate after 0.5 M TFA hydrolysis was confirmed as the polysaccharide backbone, and it was further analyzed by 1D and 2D NMR spectroscopy. Thus, the structural elucidation of AGSP was accomplished, and it has a backbone of →4)-β-GlcA(1→2)-α-Man(1→ repeating unit with Fuc, Xyl and Gal in the branch. The analytical strategy demonstrated was useful to characterize the structure of polysaccharides.
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Hong-xu
authors.initials: HX
authors.lastname: Wang
authors.affiliation: National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Li
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China. Electronic address: songs1008@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.affiliation: National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, PR China; School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Ying-huan
authors.initials: YH
authors.lastname: Fu
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
authors.forename: Li-peng
authors.initials: LP
authors.lastname: Zhang
mesh_terms: D000983:Antipyrine / Q000031:analogs & derivatives* / Q000737:chemistry; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D000077553:Edaravone; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000737:chemistry*; D014574:Uronic Acids / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D014574:Uronic Acids; D000077553:Edaravone; D000983:Antipyrine
keywords: Abalone; Acid hydrolysis; Disaccharide; PMP derivatization; Polysaccharide backbone