title: Cardiotoxicity induced by dietary oxidized sunflower oil in rats: pro- and antioxidant effects of α-tocopherol.
abstract: This study highlighted the pro-oxidative functions of α-tocopherol (αT) on the heart antioxidant system and tissue histopathology of oxidized sunflower oil (OSO)-exposed rats.Four groups of male Wistar rats were fed with different diets: 1) control diet containing FSO (fresh sunflower oil); 2) diet containing 5 % OSO; 3) diet containing 5 % OSO, supplemented with 600 mg αT kg-1; and 4) diet containing 5 % OSO, supplemented with 1200 mg αT kg-1. The hearts were then isolated, and the antioxidant enzymatic activities were assessed. Body weight and catalase (CAT), glutathione peroxidase (GPx) activities significantly decreased in groups fed with OSO, while the lipid peroxidation (LPO) level significantly increased. Administration of OSO with αT (600 mg · kg-1) returned the body weight values and LPO levels to similar values as the control group. The CAT and GPx activities increased but remained significantly lower compared to the control group. In the OSO group with αT (1200 mg · kg-1), the CAT and GPx activities also decreased, while LPO significantly increased. Heart tissue sections obtained from the groups revealed the presence of large areas of necrosis. This study suggested that OSO induced oxidative stress and that administration of a moderate dose of αT restored the antioxidant balance, but that high levels of αT supplementation result in a pro-oxidant effect.
authors.affiliation: University, Blida, Algeria, and Ecole Nationale Supérieure dAgronomie, Food Technology Department, El Harrach, Algiers, Algeria.
authors.forename: Fayrouz
authors.initials: F
authors.lastname: Rouaki
authors.affiliation: Institut National de la Recherche Agronomique dAlgérie (INRAA), Department/Unit: Food Technology, Algiers, Algeria.
authors.forename: Azzedine
authors.initials: A
authors.lastname: Mazari
authors.affiliation: University, Blida, Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Kanane
authors.affiliation: University, Blida, Algeria, and Department of Chemistry, Faculty of Sciences, Blida.
authors.forename: Mohamed Brahim
authors.initials: MB
authors.lastname: Errahmani
authors.affiliation: Ecole Nationale Supérieure dAgronomie, Food Technology Department, El Harrach, Algiers, Algeria.
authors.forename: Ali
authors.initials: A
authors.lastname: Ammouche
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D054715:Cardiotoxins; D002374:Catalase / Q000037:antagonists & inhibitors; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005979:Glutathione Peroxidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000473:pathology; D016877:Oxidants / Q000008:administration & dosage; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000009:adverse effects* / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D000074242:Sunflower Oil; D024502:alpha-Tocopherol / Q000008:administration & dosage* / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D054715:Cardiotoxins; D004042:Dietary Fats, Unsaturated; D016877:Oxidants; D010938:Plant Oils; D000074242:Sunflower Oil; D002374:Catalase; D005979:Glutathione Peroxidase; D024502:alpha-Tocopherol
keywords: heart; histopathology; oxidized sunflower oil; rat; toxicity; α-tocopherol