title: Thiolated pyrimidine nucleotides may interfere thiol groups concentrated at lipid rafts of HIV-1 infected cells.
abstract: Upon HIV infection, cells become activated and cell surface thiols are present in increased number. Earlier we demonstrated in vitro anti-HIV effect of thiolated pyrimidine nucleotide UD29, which interferes thiol function. To further analyse the redox processes required for HIV-1 entry and infection, toxicity assays were performed using HIV-1 infected monolayer HeLaCD4-LTR/ β-gal cells and suspension H9 T cells treated with several thiolated nucleotide derivatives of UD29. Selective cytotoxicity of thiolated pyrimidines on HIV-1 infected cells were observed. Results indicate that thiolated pyrimidine derivates may interfere with -SH (thiol) groups concentrated in lipid rafts of cell membrane and interacts HIV-1 infected (activated) cells resulting in a selective cytotoxicity of HIV-1 infected cells, and reducing HIV-1 entry.
authors.affiliation: Semmelweis University Institute of Medical Microbiology Budapest Hungary.
authors.forename: Szilvia
authors.initials: S
authors.lastname: Kanizsai
authors.affiliation: Semmelweis University Institute of Medical Microbiology Budapest Hungary.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ongrádi
authors.affiliation: Debrecen University Department of Biochemistry and Molecular Biology Debrecen Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Aradi
authors.affiliation: Semmelweis University Institute of Medical Microbiology Budapest Hungary.
authors.forename: Károly
authors.initials: K
authors.lastname: Nagy
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D021962:Membrane Microdomains / Q000737:chemistry / Q000187:drug effects*; D011742:Pyrimidine Nucleotides / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D011742:Pyrimidine Nucleotides; D013438:Sulfhydryl Compounds
keywords: HIV infection; cell surface redox system; entry inhibitors; lipid rafts; thiolated nucleotides