title: Spore photoproduct lyase: the known, the controversial, and the unknown.
abstract: Spore photoproduct lyase (SPL) repairs 5-thyminyl-5,6-dihydrothymine, a thymine dimer that is also called the spore photoproduct (SP), in germinating endospores. SPL is a radical S-adenosylmethionine (SAM) enzyme, utilizing the 5'-deoxyadenosyl radical generated by SAM reductive cleavage reaction to revert SP to two thymine residues. Here we review the current progress in SPL mechanistic studies. Protein radicals are known to be involved in SPL catalysis; however, how these radicals are quenched to close the catalytic cycle is under debate.
authors.affiliation: From the Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, Indiana, 46202 and.
authors.forename: Linlin
authors.initials: L
authors.lastname: Yang
authors.affiliation: From the Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, Indiana, 46202 and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine (IUSM), Indianapolis, Indiana 46202 lilei@iupui.edu.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
mesh_terms: D005609:Free Radicals / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000737:chemistry / Q000378:metabolism*; D013171:Spores, Bacterial
chemical_list: D005609:Free Radicals; D011506:Proteins; D012436:S-Adenosylmethionine; C088014:spore photoproduct lyase
keywords: DNA Repair; DNA-Protein Interaction; Enzyme Catalysis; Enzyme Mechanism; Radiation Biology; Radical; Redox