title: Can ischemia-modified albumin be used as an objective biomarker for renal ischemic damage? An experimental study with Wistar albino rats.
abstract: BACKGROUND
  This study sought to evaluate the correlation of ischemia-modified albumin (IMA) with time-dependent renal ischemic injury.
  
  
  METHODS
  We established 5 groups of 8 Wistar albino rats as follows: sham, 10 minutes of renal ischemia, 20 minutes of renal ischemia, 30 minutes of renal ischemia, and 40 minutes of renal ischemia. Renal ischemia was established by occlusion of the right renal pedicle. Blood samples were obtained after exploration of the renal pedicle in the sham group and after thoracotomy and directly from the cardiac chambers at the end of the ischemic period in the other groups. The ischemic kidneys were removed for histopathological evaluation, and the rats were killed.
  
  
  RESULTS
  There were significant differences among the IMA levels of the 5 groups (P = .0013). Pathological examination showed that renal ischemic injury corresponded to the duration of ischemia. In the group analysis, the pathological evaluation scores were significantly different among the groups (P < .001).
  
  
  CONCLUSIONS
  This study shows that IMA levels can be used as a nonselective biomarker for renal ischemic injury. However, further studies are needed to support our findings.
authors.affiliation: Department of Urology, Necmi Ayanoglu Silivri State Hospital, Istanbul, Turkey.
authors.forename: H
authors.initials: H
authors.lastname: Kocan
authors.affiliation: Department of Urology, Necmi Ayanoglu Silivri State Hospital, Istanbul, Turkey. Electronic address: drsinharib@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Citgez
authors.affiliation: Department of Urology, Istanbul Training and Research Hospital, Istanbul, Turkey.
authors.forename: U
authors.initials: U
authors.lastname: Yucetas
authors.affiliation: Department of Biochemistry, Haseki Training and Research Hospital, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Yucetas
authors.affiliation: Department of Urology, Istanbul Training and Research Hospital, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Yazici
authors.affiliation: Department of Urology, Istanbul Medical Faculty, Istanbul, Turkey.
authors.forename: A S
authors.initials: AS
authors.lastname: Amasyali
authors.affiliation: Department of Urology, Istanbul Training and Research Hospital, Istanbul, Turkey.
authors.forename: E
authors.initials: E
authors.lastname: Unluer
authors.affiliation: Department of Urology, Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.
authors.forename: A I
authors.initials: AI
authors.lastname: Tasci
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D007674:Kidney Diseases / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human
chemical_list: D015415:Biomarkers; D012709:Serum Albumin; C561522:ischemia-modified albumin; D000075462:Serum Albumin, Human