title: Inhibition of AMPK expression in skeletal muscle by systemic inflammation in COPD rats.
abstract: BACKGROUND
  Chronic obstructive pulmonary disease (COPD) is a disease characterized by airflow limitation and inflammation. Meanwhile, COPD also is associated with metabolic disorders, such as skeletal muscle weakness. Strikingly, activation of AMP-activated protein kinase (AMPK) exerts critical roles in energy metabolism. However, it remains unclear whether and how the expression levels of AMPK are affected in the COPD model rats which may lead to the dysfunction of the skeletal muscle in these rats.
  
  
  METHODS
  Here we developed a rat model of COPD, and we investigated the morphological changes of peripheral skeletal muscle and measured the levels of tumor necrosis factor -α (TNF-α) and AMPK in skeletal muscle by using approaches that include immunohistochemistry and polymerase chain reaction (PCR).
  
  
  RESULTS
  We found that the expression levels of both AMPK mRNA and protein in skeletal muscles were significantly reduced in the COPD model rats, in comparison to those from the control rats, the COPD model rats that received treatments with AICAR and resveratrol, whereas the expression levels of TNF-α were elevated in COPD rats.
  
  
  CONCLUSIONS
  Such findings indicate that AMPK may serve as a target for therapeutic intervention in the treatment of muscle weakness in COPD patients.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. rose20000@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. yyl971002@yahoo.com.
authors.forename: Jun-yi
authors.initials: JY
authors.lastname: Shang
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. malijun0401@163.com.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Ma
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. liuhongshan@163.com.
authors.forename: Bei-bei
authors.initials: BB
authors.lastname: Sun
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. yyl971002@icloud.com.
authors.forename: Xin-gang
authors.initials: XG
authors.lastname: Hu
authors.affiliation: Department of Respiratory and Critical Care Medicine, Zhengzhou University People's Hospital (He'nan Provincial People's Hospital), 7 Weiwu Road, ZhengZhou, 450003, China. liubaoyang1975@126.com.
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe East Road, ZhengZhou, 450003, China. zgj@zzu.edu.cn.
authors.forename: Guo-jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000620:Aminoimidazole Carboxamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D015536:Down-Regulation; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D018908:Muscle Weakness / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D000077185:Resveratrol; D012265:Ribonucleotides / Q000494:pharmacology; D056564:Sirtuin 1 / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D012333:RNA, Messenger; D012265:Ribonucleotides; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; D000620:Aminoimidazole Carboxamide; D055372:AMP-Activated Protein Kinases; C488368:Sirt1 protein, rat; D056564:Sirtuin 1; C031143:AICA ribonucleotide; D000077185:Resveratrol