title: Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains.
abstract: Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Stephanie C
authors.initials: SC
authors.lastname: Whittle
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Seyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Hannah X
authors.initials: HX
authors.lastname: Chu
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Shenpeng R
authors.initials: SR
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Zihui
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore Singapore, Singapore ; School of Pharmacy, Sungkyunkwan University Suwon, South Korea ; School of Biomedical Sciences, The University of Queensland St Lucia, QLD, Australia.
authors.forename: Thiruma V
authors.initials: TV
authors.lastname: Arumugam
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia.
authors.forename: Anthony
authors.initials: A
authors.lastname: Vinh
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia ; Department of Surgery, Monash Medical Centre, Southern Clinical School, Monash University Clayton, VIC, Australia.
authors.forename: Grant R
authors.initials: GR
authors.lastname: Drummond
authors.affiliation: Department of Pharmacology, Monash University Clayton, VIC, Australia ; Department of Surgery, Monash Medical Centre, Southern Clinical School, Monash University Clayton, VIC, Australia.
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Sobey
keywords: Th1/Th2 balance; cerebral ischemia-reperfusion; immune cell infiltration; inflammation; middle cerebral artery occlusion; stroke