title: MicroRNA-145 inhibits lung cancer cell metastasis.
abstract: Previous studies have identified a variety of microRNAs (miRNAs) that have important roles in cancer progression, particularly in tumor invasion and metastasis. Downregulation of miR‑145 was reported to occur in various types of human cancer; however, the role of miR‑145 in lung cancer metastasis and its potential mechanisms of action remain to be elucidated. The present study aimed to investigate the effects of miR‑145 on metastasis and epithelial‑mesenchymal transition (EMT) in A549 human lung adenocarcinoma cells. In addition, the underlying mechanisms by which miR‑145 regulates EMT were examined. The miR‑145 mimic was transfected into A549 cells; cell invasion and adhesion assays were then performed in order to investigate cell metastasis, and western blot analysis was used to examine the expression of EMT markers. In order to further examine the underlying mechanisms by which miR‑145 regulates EMT, a luciferase reporter assay was performed to determine whether miR‑145 targeted Oct4. In addition, the expression of Wnt3a and β‑catenin in A549 cells was measured following transfection with small hairpin RNA‑Oct4. To the best of our knowledge, the results of the present study demonstrated for the first time, that miR‑145 inhibited lung cancer cell metastasis and EMT via targeting the Oct4 mediated Wnt/β‑catenin signaling pathway.
authors.affiliation: Department of General Surgery, Ministry of Health Hepatobiliary and Enteric Surgery Center, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Ling
authors.affiliation: Department of General Surgery, Ministry of Health Hepatobiliary and Enteric Surgery Center, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Zhong-Shu
authors.initials: ZS
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Ministry of Health Hepatobiliary and Enteric Surgery Center, Xiangya School of Medicine, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Yang-De
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Qian-De
authors.initials: QD
authors.lastname: Liao
authors.affiliation: Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Jian-Xiong
authors.initials: JX
authors.lastname: Feng
authors.affiliation: Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Xue-Yu
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Jiangxi Provincial Chest Hospital, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Tian-Sheng
authors.initials: TS
authors.lastname: Shi
mesh_terms: D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D009362:Neoplasm Metastasis; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D060509:Wnt3A Protein / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; D060509:Wnt3A Protein; D051176:beta Catenin