title: Comparison of glucose oxidases from Penicillium adametzii, Penicillium Funiculosum and Aspergillus Niger in the design of amperometric glucose biosensors.
abstract: The properties of amperometric glucose biosensors based on three different glucose oxidases and various redox mediators were evaluated. Glucose oxidases (GOx) from Penicillium adametzii, Penicillium funiculosum and Aspergillus niger and artificial redox mediators, such as ferrocene, ferrocenecarboxaldehyde, α-methylferrocene methanol and ferrocenecarboxylic acid, were used for modifying the graphite rod electrode and amperometrical reagent-less glucose detection. The obtained results were compared using N-methylphenazonium methyl sulphate in the solution. Taking into account the experimental kinetic parameters and the stability of the tested enzymatic electrodes, GOx from Penicillium funiculosum proved to be more suitable for glucose biosensor design in comparison with other evaluated enzymes.
authors.affiliation: Department of Physical Chemistry, Faculty of Chemistry, Vilnius University.
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Voronovic
authors.forename: Tatiana
authors.initials: T
authors.lastname: Semashko
authors.forename: Raisa
authors.initials: R
authors.lastname: Mikhailova
authors.forename: Asta
authors.initials: A
authors.lastname: Kausaite-Minkstimiene
authors.forename: Almira
authors.initials: A
authors.lastname: Ramanaviciene
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D015374:Biosensing Techniques; D004566:Electrodes; D005947:Glucose / Q000032:analysis* / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*
chemical_list: D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose