title: Preparation of temperature sensitive molecularly imprinted polymer for solid-phase microextraction coatings on stainless steel fiber to measure ofloxacin.
abstract: A kind of new temperature sensitive molecularly imprinted polymer (MIP) with ofloxacin (OFL) as template was prepared for the coating of solid phase microextraction (SPME). Dopamine was self-polymerized on stainless steel fiber (SSF) as the SPME support followed by silanization. Then MIP was synthesized as SPME coating on the modified SSF in a capillary, with N-isopropyl acrylamide as temperature sensitive monomer and methacrylic acid as functional monomer. The synthesis could be well repeated with multiple capillaries putting in the same reaction solution. The obtained MIP fiber was evaluated in detail with different techniques and various adsorption experiments. At last the MIP fiber was used to extract the OFL in milk. Satisfied recoveries between 89.7 and 103.4% were obtained with the limit of quantification (LOQLC) of 0.04 μg mL(-1) by the method of SPME coupled with high performance of liquid chromatography (HPLC).
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Guan
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wanjin
authors.initials: W
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China. Electronic address: zhanghx@lzu.edu.cn.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting; D015242:Ofloxacin / Q000032:analysis* / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013193:Stainless Steel / Q000737:chemistry*; D013696:Temperature
chemical_list: D011108:Polymers; D013193:Stainless Steel; D015242:Ofloxacin
keywords: Molecularly imprinted polymer; Ofloxacin; Solid phase microextraction; Temperature sensitive