title: Hypoxic X-irradiation as an external stimulus for conformational change of oligodeoxynucleotides that possess disulfide bond and regulation of DNAzyme function.
abstract: We achieved a conformational change of oligodeoxynucleotides and the regulation of DNAzyme function by means of a radiolytic strand exchange reaction of disulfide bond. We designed a system in which the DNAzyme function of RNA cleavage was suppressed by the hybridization of an inhibitor strand that possessed disulfide bond with an active DNAzyme. Hypoxic X-irradiation led to the recovery of RNA cleavage because the strand exchange reaction at the disulfide bond in inhibitor strand resulted in a release of inhibitor strand. This strategy may be applicable to gene regulation by hypoxic X-irradiation.
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan. Electronic address: tanabeka@scl.kyoto-u.ac.jp.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tanabe
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Kana
authors.initials: K
authors.lastname: Okada
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Sugiura
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Ito
authors.affiliation: Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Sei-Ichi
authors.initials: S
authors.lastname: Nishimoto
mesh_terms: D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry*; D006801:Humans; D000860:Hypoxia; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000528:radiation effects*; D010084:Oxidation-Reduction; D014965:X-Rays
chemical_list: D021881:DNA, Catalytic; D004220:Disulfides; D009838:Oligodeoxyribonucleotides
keywords: DNAzyme; Disulfide bond; Hypoxic X-irradiation; Oligodeoxynucleotides