title: Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen/thawed chicken sperm variables.
abstract: This work examines the effect of the interaction between different concentrations of two cryoprotectants - glycerol (GLY) and dimethylacetamide (DMA) - and two methods of cryopreservation - pellets produced by plunging into liquid nitrogen and gradual in-straw freezing - on frozen/thawed chicken sperm variables. Sperm was cryopreserved using: (i) 6% DMA, following the in-straw and the pellet methods (ii) 11% GLY, following the in-straw and the pellet methods; and (iii) 8% GLY in the in-straw method and 3% DMA in the pellet method (i.e. reduced cryoprotectant concentrations). When 6% DMA was used as the cryoprotectant, no differences were seen between the in-straw and pellet methods in terms of frozen/thawed sperm variables or fertility (10.8% and 12.8%, respectively). The viability and motility variables of the frozen/thawed sperm produced using the in-straw method with 11% GLY were higher (p < 0.05) than those recorded for the sperm preserved using the same cryoprotectant and concentration in the pellet method. However, fertility was extremely low in both groups (2.1% and 4.2% for the in-straw and pellet methods, respectively). Finally, the use of 8% GLY in the in-straw method returned higher sperm viability, intact acrosome and motility values than the use of 3% DMA in the pellet method (p < 0.01). No differences were seen, however, in the fertility results obtained (28.8% and 25.0%, respectively). These results suggest that cryoprotectant concentrations can be reduced and still provide acceptable fertility rates.
authors.affiliation: Department of Animal Reproduction, INIA, Madrid, Spain; Department of Poultry Production, Faculty of Agriculture, Assiut University, Egypt.
authors.forename: F M K
authors.initials: FM
authors.lastname: Abouelezz
authors.forename: C
authors.initials: C
authors.lastname: Castaño
authors.forename: A
authors.initials: A
authors.lastname: Toledano-Díaz
authors.forename: M C
authors.initials: MC
authors.lastname: Esteso
authors.forename: A
authors.initials: A
authors.lastname: López-Sebastián
authors.forename: J L
authors.initials: JL
authors.lastname: Campo
authors.forename: J
authors.initials: J
authors.lastname: Santiago-Moreno
mesh_terms: D000081:Acetamides / Q000008:administration & dosage; D000818:Animals; D002470:Cell Survival; D002645:Chickens; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005298:Fertility / Q000187:drug effects; D005990:Glycerol / Q000008:administration & dosage; D006358:Hot Temperature; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
chemical_list: D000081:Acetamides; D003451:Cryoprotective Agents; C013959:dimethylacetamide; D005990:Glycerol