title: Synergistic effect of interleukin-6 and hyaluronic acid on cell migration and ERK activation in human keratinocytes.
abstract: Wound healing is initiated and progressed by complex integrated process of cellular, physiologic, and biochemical events, such as inflammation, cell migration and proliferation. Interleukin 6 (IL-6) is a multifunctional cytokine, and it could regulate the inflammatory response of wound healing process in a timely manner. Hyaluronic acid (HA) is an essential component of the extracellular matrix, and contributes significantly to cell proliferation and migration. The purpose of this study was to investigate the effects of IL-6 or/and HA on the cell migration process in human keratinocytes. Combining IL-6 and HA significantly increased the cell migration in scratch based wound healing assay. The phosphorylation of extracellular-signal-regulated kinase (ERK) was significantly increased after 1 hr of IL-6 and HA treatment, but the phosphorylation of p38 mitogen-activated protein kinase (MAPK) was not. We also found that significant increase of the NF-κB translocation from cytoplasm into nucleus after 1 hr of IL-6 or/and HA treatments. This study firstly showed that synergistic effects of combining IL-6 and HA on the cell migration of wound healing by activation of ERK and NF-κB signaling. Further studies might be required to confirm the synergistic effects of HA and IL-6 in the animal model for the development of a novel therapeutic mixture for stimulation of wound healing process.
authors.affiliation: Eulji Medi-Bio Research Institute, Eulji General Hospital, Eulji University, Seoul, Korea.
authors.forename: Jee-Hyun
authors.identifier: 0000-0002-6730-7854
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Eulji Medi-Bio Research Institute, Eulji General Hospital, Eulji University, Seoul, Korea. ; Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Seongnam, Korea. ; Department of Biomedical Laboratory Science, Eulji University, Seongnam, Korea.
authors.forename: Jin Hyun
authors.identifier: 0000-0001-9898-4471
authors.initials: JH
authors.lastname: Jun
authors.affiliation: Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Seongnam, Korea.
authors.forename: Ji Hyun
authors.identifier: 0000-0002-2466-1925
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Senior Healthcare, BK21 plus Program, Graduated School, Eulji University, Seongnam, Korea. ; Department of Biomedical Laboratory Science, Eulji University, Seongnam, Korea.
authors.forename: Ho Joong
authors.identifier: 0000-0002-5614-1350
authors.initials: HJ
authors.lastname: Sung
authors.affiliation: Eulji Medi-Bio Research Institute, Eulji General Hospital, Eulji University, Seoul, Korea. ; Department of Plastic and Reconstructive Surgery, Eulji General Hospital, School of Medicine, Eulji University, Seoul, Korea.
authors.forename: Jong Hun
authors.identifier: 0000-0001-6417-6986
authors.initials: JH
authors.lastname: Lee
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D015850:Interleukin-6 / Q000494:pharmacology*; D015603:Keratinocytes / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D021381:Protein Transport / Q000187:drug effects; D014945:Wound Healing; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; D016328:NF-kappa B; D006820:Hyaluronic Acid; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Cell Movement; ERK; Hyaluronic Acid; Interleukin-6; Keratinocytes; Phosphorylation; Wound Healing