title: Magnetically labeled feeder system for mouse pluripotent stem cell culture.
abstract: We report here a magnetically labeled feeder system for mouse embryonic stem/induced pluripotent stem (ES/iPS) cells. Magnetic attraction of feeder cells labeled with magnetite nanoparticles significantly increased ES/iPS colony-forming efficiency. Magnetic labeling of feeder cells also facilitated separation of ES/iPS cells from feeder cells.
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Horie
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ito
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Maki
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Kawabe
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: kamihira@chem-eng.kyushu-u.ac.jp.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Kamihira
mesh_terms: D000818:Animals; D015820:Cadherins / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D061252:Feeder Cells / Q000166:cytology*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000032:analysis* / Q000737:chemistry; D051379:Mice; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D015820:Cadherins; D058185:Magnetite Nanoparticles
keywords: Cell separation; E-cadherin; Feeder cells; Magnetite nanoparticles; Pluripotent stem cells