title: Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes.
abstract: The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.
authors.affiliation: a Center for Healthy Aging; Department of Cellular and Molecular Medicine ; University of Copenhagen ; Copenhagen , Denmark.
authors.forename: Nicolai B
authors.initials: NB
authors.lastname: Larsen
authors.forename: Ian D
authors.initials: ID
authors.lastname: Hickson
authors.forename: Hocine W
authors.initials: HW
authors.lastname: Mankouri
mesh_terms: D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D059767:Recombinational DNA Repair; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: C062011:DNA replication terminus site-binding protein, E coli; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C489236:tus protein, E coli; D004265:DNA Helicases
keywords: DnaB helicase; MCM helicase; RecQ helicase; homologous recombination repair; replication fork