title: Production of Ban miniature pig embryos by in vitro fertilization: a comparative study with Landrace.
abstract: Ban is an endangered miniature pig breed in Vietnam. This study aimed to set up an in vitro embryo production (IVP) system for this breed. Ban's epididymal sperm concentration (1240 ± 35 × 10(6) /mL) was lower (P < 0.01) compared with Landrace (4160 ± 42 × 10(6) /mL). However, sperm characteristics before and after freezing in Ban and Landrace were similar. The numbers of follicles with diameter larger than 2 mm per ovary in Ban females treated with equine chorionic gonadotropin and human chorionic gonadotropin (27.1 ± 1.3) were higher (P < 0.05) than those in Landrace (12.9 ± 2.0) and in non-hormone stimulated Ban (no > 2 mm follicles). After in vitro maturation, the percentages of oocytes with expanded cumulus cells and the first polar body (matured oocytes) were not different among Ban, hormone-stimulated Ban and Landrace. The percentages of two-cell embryos and morulae derived from oocytes collected from three sources did not differ. However, the rate of blastocysts derived from oocytes in non-stimulated Ban (4.0 ± 3.8%) was lower (P < 0.05) than that in Landrace (15.3 ± 1.8%). In conclusion, an effective IVP system for good quality embryos in Ban, that is essential for genetic conservation of this breed, was established.
authors.affiliation: Laboratory of Embryo Biotechnology and Stem Cells, Institute of Biotechnology, Vietnamese Academy of Science and Technology, Hanoi, Vietnam.
authors.forename: Bui Xuan
authors.initials: BX
authors.lastname: Nguyen
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Uoc
authors.forename: Thanh Quang
authors.initials: TQ
authors.lastname: Dang-Nguyen
authors.forename: Nguyen Viet
authors.initials: NV
authors.lastname: Linh
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Men
authors.forename: Trung Thanh
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D001755:Blastocyst; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004822:Epididymis / Q000166:cytology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D059471:In Vitro Oocyte Maturation Techniques; D008297:Male; D009865:Oocytes; D006080:Ovarian Follicle; D012662:Semen Preservation; D013076:Sperm Count; D013094:Spermatozoa; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics
chemical_list: D006063:Chorionic Gonadotropin
keywords: Ban miniature pig; frozen semen; hormone-stimulated oocyte; in vitro fertilization