title: Separation and purification of both tea seed polysaccharide and saponin from camellia cake extract using macroporous resin.
abstract: A novel method to separate and purify tea seed polysaccharide and tea seed saponin from camellia cake extract by macroporous resin was developed. Among four kinds of resins (AB-8, NKA-9, XDA-6, and D4020) tested, AB-8 macroporous resin possessed optimal separating capacity for the two substances and thus was selected for the separation, in which deionized water was used to elute tea seed polysaccharide, 0.25% NaOH solution to remove the undesired pigments, and 90% ethanol to elute tea seed saponin. Further dynamic adsorption/desorption experiments on AB-8 resin-based column chromatography were conducted to obtain the optimal parameters. Under optimal dynamic adsorption and desorption conditions, 18.7 and 11.8% yield of tea seed polysaccharide and tea seed saponin were obtained with purities of 89.2 and 96.0%, respectively. The developed method provides a potential approach for the large-scale production of tea seed polysaccharide and tea seed saponin from camellia cake.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, China.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Yang
authors.forename: Mingda
authors.initials: M
authors.lastname: Zhou
authors.forename: Chengyun
authors.initials: C
authors.lastname: Zhou
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Fangfang
authors.initials: F
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
keywords: Macroporous resin; Purification; Tea seed polysaccharide; Tea seed saponins