title: Preparation of a cross-linked porous protein crystal containing Ru carbonyl complexes as a CO-releasing extracellular scaffold.
abstract: Protein crystals generally are stable solid protein assemblies. Certain protein crystals are suitable for use as nanovessels for immobilizing metal complexes. Here we report the preparation of ruthenium carbonyl-incorporated cross-linked hen egg white lysozyme crystals (Ru·CL-HEWL). Ru·CL-HEWL retains a Ru carbonyl moiety that can release CO, although a composite of Ru carbonyl-HEWL dissolved in buffer solution (Ru·HEWL) does not release CO. We found that treatment of cells with Ru·CL-HEWL significantly increased nuclear factor kappa B (NF-κB) activity as a cellular response to CO. These results demonstrate that Ru·CL-HEWL has potential for use as an artificial extracellular scaffold suitable for transport and release of a gas molecule.
authors.affiliation: Graduate School of Engineering, Kyoto University, Katsura , Nishikyo-ku, Kyoto 615-8510, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Tabe
authors.forename: Kenta
authors.initials: K
authors.lastname: Fujita
authors.forename: Satoshi
authors.initials: S
authors.lastname: Abe
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tsujimoto
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kuchimaru
authors.forename: Shinae
authors.initials: S
authors.lastname: Kizaka-Kondoh
authors.forename: Mikio
authors.initials: M
authors.lastname: Takano
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitagawa
authors.forename: Takafumi
authors.initials: T
authors.lastname: Ueno
mesh_terms: D000818:Animals; D001692:Biological Transport; D002248:Carbon Monoxide / Q000737:chemistry* / Q000494:pharmacology; D002645:Chickens; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray; D004529:Egg White / Q000737:chemistry; D015870:Gene Expression / Q000187:drug effects; D017930:Genes, Reporter; D005976:Glutaral / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D009113:Muramidase / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D016062:Porosity; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D056831:Coordination Complexes; D003432:Cross-Linking Reagents; D009211:Myoglobin; D016328:NF-kappa B; D002248:Carbon Monoxide; D012428:Ruthenium; D008156:Luciferases; D009113:Muramidase; D005976:Glutaral