title: Improved determination of malonaldehyde by high-performance liquid chromatography with UV detection as 2,3-diaminonaphthalene derivative.
abstract: A rapid, specific and simple procedure is proposed for the determination of free malonaldehyde (MA) contained in fish tissue. The method is the optimization of the reaction of MA with 2,3-diaminonaphthalene to afford a naphtodiazepinium ion that present a UV absorption at 311nm, useful for MA determination by HPLC with UV detection. The reaction proceeds in the presence of 25% acetonitrile at 37°C in 20min at pH 2 using 2,4-pentanedione as internal standard. The method has been applied to homogenized samples of canned mackerel fillets that were treated with 2,3-diaminonaphthalene in an acidic aqueous:acetonitrile mixture. The produced naphtodiazepinium ion was extracted in acetonitrile by a salting-out homogeneous liquid-liquid extraction. A standard calibration was carried out in the range 0.625-10nmol/g. The reliability of the procedure is demonstrated by linearity (r(2)=0.998), limit of detection (0.16nmol/g), limit of quantification (0.22nmol/g), repeatibility (RSD 5.57%), and intermediate precision (RSD 8.92%).
authors.affiliation: Department of Veterinary Sciences and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Panseri
authors.affiliation: Department of Veterinary Sciences and Public Health, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy.
authors.forename: Luca Maria
authors.initials: LM
authors.lastname: Chiesa
authors.affiliation: Department of Health Sciences, Università degli Studi di Milano, Via Di Rudinì 8, 20142 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Brizzolari
authors.affiliation: Department of Health Sciences, Università degli Studi di Milano, Via Di Rudinì 8, 20142 Milan, Italy.
authors.forename: Enzo
authors.initials: E
authors.lastname: Santaniello
authors.affiliation: Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Passerò
authors.affiliation: Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, via Celoria 10, 20133 Milan, Italy. Electronic address: pierantonio.biondi@unimi.it.
authors.forename: Pier Antonio
authors.initials: PA
authors.lastname: Biondi
mesh_terms: D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008315:Malondialdehyde / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: C026373:2,3-diaminonaphthalene; D008315:Malondialdehyde; D015081:2-Naphthylamine
keywords: 2,3-Diaminonaphthalene; Canned mackerel fillet; HPLC-UV; Malonaldehyde