title: Analysis of HLA-G expression in serum and biopsy samples of kidney transplant recipients.
abstract: In this study, we analyzed HLA-G expression in serum and graft biopsies of renal transplant patients to find out whether there is any relationship between HLA-G and renal graft acceptance. The transplant patients were divided into two groups: those without any rejection episode (n=32) and those with acute rejection (n=33). Patient sera were collected 1 day before and at various intervals after transplantation. Soluble HLA-G (sHLA-G) in serum was determined using ELISA. In time-course experiment we found that in all patients (with and without rejection) the pre-transplantation level of sHLA-G declined in the early post-transplant period (1-2 weeks). In sera collected over 1-12 months after transplantation, a substantial increase of sHLA-G was detected in patients without rejection while no change or additional decline was observed in recipients with graft rejection. In sera collected after more than 1 year post-transplantation, sHLA-G levels increased in both groups of patients (with or without graft rejection). The time-course of serum sHLA-G antigens in patients with graft rejection was in good correlation with the course of total HLA-G mRNA determined in graft biopsy samples isolated from patients with acute rejection. We further demonstrated that serum sHLA-G values were significantly higher in patients without graft rejection than with rejection (P=0.0058). This observation supports the assumption that the increase of serum sHLA-G may contribute to allograft acceptance.
authors.affiliation: Cancer Research Institute, SAS, Vlárska7, 83391 Bratislava, Slovak Republic.
authors.forename: K
authors.initials: K
authors.lastname: Poláková
authors.affiliation: National Transplantation Organisation, Limbová 12, Bratislava, Slovak Republic.
authors.forename: H
authors.initials: H
authors.lastname: Bandžuchová
authors.affiliation: Department of Urology with Kidney Transplant Centre, University Hospital Bratislava, Bratislava, Slovak Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Žilinská
authors.affiliation: National Transplantation Organisation, Limbová 12, Bratislava, Slovak Republic.
authors.forename: S
authors.initials: S
authors.lastname: Chreňová
authors.affiliation: National Transplantation Organisation, Limbová 12, Bratislava, Slovak Republic.
authors.forename: D
authors.initials: D
authors.lastname: Kuba
authors.affiliation: Institute of Virology, SAS, Dúbravská cesta 9, Bratislava, Slovak Republic. Electronic address: virugrus@savba.sk.
authors.forename: G
authors.initials: G
authors.lastname: Russ
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015870:Gene Expression; D006084:Graft Rejection / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D059951:HLA-G Antigens / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D006648:Histocompatibility; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007668:Kidney / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D066027:Transplant Recipients
chemical_list: D059951:HLA-G Antigens; D007166:Immunosuppressive Agents
keywords: HLA-G antigen; HLA-G transcript; Kidney transplantation; Soluble HLA-G