title: Ezakiella peruensis gen. nov., sp. nov. isolated from human fecal sample from a coastal traditional community in Peru.
abstract: A novel Gram-stain positive, non-motile, non-sporeforming coccus-shaped, obligately anaerobic bacterium was isolated from a fecal sample of an individual residing in a traditional Peruvian community. The organism was characterized using biochemical, chemotaxonomic and phylogenetic methods. Comparative 16S rRNA gene sequence analyses and phenotypic characteristics demonstrated that the organism was biochemically and phenotypically related, but distinct, from a group of organisms referred to as the Gram-stain positive anaerobic cocci (GPAC). The major cellular fatty acids of the novel isolate were determined to be C16:0 (18.3%), C18:1ω9c (39.8%), C18:2ω6,9c/C18:0 ANTE (13.2%). Fermentation end products from PYG are acetate and formate. Cell-wall peptidoglycan was found to be A4α (L-Lys-L-Ala-L-Glu) and the G + C content was determined to be 38.4 mol%. Based on the phenotypic, chemotaxonomic, and phylogenetic results, Ezakiella peruensis gen. nov., sp. nov., is now proposed. The type strain is M6.X2(T) (DSM 27367(T) = NBRC 109957 (T) = CCUG 64571(T)).
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, USA.
authors.forename: Nisha B
authors.initials: NB
authors.lastname: Patel
authors.affiliation: Department of Anthropology, University of Oklahoma, USA; Universidad Cientifica del Sur, Lima, Peru.
authors.forename: Raul Y
authors.initials: RY
authors.lastname: Tito
authors.affiliation: Department of Anthropology, University of Oklahoma, USA; Universidad Cientifica del Sur, Lima, Peru.
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Obregón-Tito
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, USA.
authors.forename: Lindsey
authors.initials: L
authors.lastname: O'Neal
authors.affiliation: Centro Nacional de Salud Intercultural, Instiuto Nacional de Salud, Lima, Peru.
authors.forename: Omar
authors.initials: O
authors.lastname: Trujillo-Villaroel
authors.affiliation: Centro Nacional de Salud Publica, Instituto Nacional de Salud, Lima, Peru.
authors.forename: Luis
authors.initials: L
authors.lastname: Marin-Reyes
authors.affiliation: Universidad Cientifica del Sur, Lima, Peru.
authors.forename: Luzmila
authors.initials: L
authors.lastname: Troncoso-Corzo
authors.affiliation: Universidad Cientifica del Sur, Lima, Peru.
authors.forename: Emilio
authors.initials: E
authors.lastname: Guija-Poma
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Uchino
authors.affiliation: Department of Anthropology, University of Oklahoma, USA.
authors.forename: Cecil M
authors.initials: CM
authors.lastname: Lewis
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma, USA. Electronic address: paul.lawson@ou.edu.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Lawson
mesh_terms: D015373:Bacterial Typing Techniques; D002353:Carrier State; D004269:DNA, Bacterial; D005243:Feces / Q000382:microbiology*; D000068536:Bacillota / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010568:Peru; D010641:Phenotype; D010802:Phylogeny; D011159:Population Surveillance; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA-phylogeny; Ezakiella peruensis gen. nov., sp. nov.; Taxonomy