title: Dissolving mechanism of strain P17 on insoluble phosphorus of yellow-brown soil.
abstract: Strain P17 was a bacterial strain identified as Bacillus megaterium isolated from ground accumulating phosphate rock powder. The fermentation broth of strain P17 and the yellow-brown soil from Nanjing Agricultural University garden were collected to conduct this study. The simulation of fixed insoluble phosphorous forms after applying calcium superphosphate into yellow-brown soil was performed in pots, while available P and total P of soil were extremely positive correlative with those of groundwater. Then the dissolving effect of strain P17 on insoluble P of yellow-brown soil was studied. Results showed that Bacillus megaterium strain P17 had notable solubilizing effect on insoluble phosphates formed when too much water-soluble phosphorous fertilizer used. During 100 days after inoculation, strain P17 was dominant. Until the 120th day, compared with water addition, available P of strain P17 inoculation treated soil increased by 3 times with calcium superphosphate addition. Besides available P, pH, activity of acid and alkaline phosphatase and population of P-solubilizing microbes were detected respectively. P-solubilizing mechanism of P-solubilizing bacteria strain P17 seems to be a synergetic effect of pH decrease, organic acids, phosphatase, etc.
authors.affiliation: College of Municipal and Environmental Engineering Shandong Jianzhu University Jinan China College of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China.
authors.forename: Chuan-qing
authors.initials: CQ
authors.lastname: Zhong
authors.affiliation: Shandong Medicinal Biotechnology Centre Jinan China Shandong Medicinal Biotechnology Centre, Jinan, China. ; State Key Laboratory of Microbial Technology Shandong University Jinan China State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
authors.forename: Guang-xiang
authors.initials: GX
authors.lastname: Cao
authors.affiliation: Life Science College Nanjing Agricultural University Nanjing China Life Science College, Nanjing Agricultural University, Nanjing, China.
authors.forename: Wei-yi
authors.initials: WY
authors.lastname: Huang
authors.affiliation: College of Municipal and Environmental Engineering Shandong Jianzhu University Jinan China College of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China.
authors.forename: Xing-she
authors.initials: XS
authors.lastname: Luan
authors.affiliation: College of Municipal and Environmental Engineering Shandong Jianzhu University Jinan China College of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, China.
authors.forename: Yi-fei
authors.initials: YF
authors.lastname: Yang
mesh_terms: D001410:Bacillus megaterium / Q000302:isolation & purification / Q000378:metabolism*; D002130:Calcium Phosphates / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D002130:Calcium Phosphates; D002264:Carboxylic Acids; D012987:Soil; D010758:Phosphorus; C494370:calcium phosphate, monobasic; D010744:Phosphoric Monoester Hydrolases
keywords: acid phosphatase; alkaline phosphatase; calcium superphosphate; p-solubilizing microbes