title: The expression of miR-125b regulates angiogenesis during the recovery of heat-denatured HUVECs.
abstract: BACKGROUND
  In previous studies we found that miR-125b was down-regulated in denatured dermis of deep partial thickness burn patients. Moreover, miR-125b inhibited tumor-angiogenesis associated with the decrease of ERBB2 and VEGF expression in ovarian cancer cells and breast cancer cells, etc. In this study, we investigated the expression patterns and roles of miR-125b during the recovery of denatured dermis and heat-denatured human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  Deep partial thickness burns in Sprague-Dawley rats and the heat-denatured cells (52°C, 35 s) were used for analysis. Western blot analysis and real-time PCR were applied to evaluate the expression of miR-125b and ERBB2 and VEGF. The ability of angiogenesis in heat-denatured HUVECs was analyzed by scratch wound healing and tube formation assay after pri-miR-125b or anti-miR-125b transfection.
  
  
  RESULTS
  miR-125b expression was time-dependent during the recovery of heat-denatured dermis and HUVECs. Moreover, miR-125b regulated ERBB2 mRNA and Protein Expression and regulated angiogenesis association with regulating the expression of VEGF in heat-denatured HUVECs.
  
  
  CONCLUSIONS
  Taken together our results show that the expression of miR-125b is time-dependent and miR-125b plays a regulatory role of angiogenesis during wound healing after burns.
authors.affiliation: Department of Burns and Reconstructive Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Situo
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Burns and Reconstructive Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Pihong
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Burns and Reconstructive Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liang
authors.affiliation: Department of Burns and Reconstructive Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China. Electronic address: huxzhongxy@163.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D020405:Dermis / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics; D006358:Hot Temperature; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D018919:Neovascularization, Physiologic / Q000235:genetics*; D011489:Protein Denaturation; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D059467:Transcriptome; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; D042461:Vascular Endothelial Growth Factor A; C531888:MIRN125 microRNA, human; C581261:MIRN125 microRNA, rat; C467484:VEGFA protein, human; C467498:vascular endothelial growth factor A, rat; C508053:ERBB2 protein, human; C483804:Erbb2 protein, rat
keywords: Angiogenesis; Denatured dermis; ERBB2; Heat-denatured Human umbilical vein endothelial cells; Vascular endothelial growth factor; miR-125b