title: shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats.
abstract: OBJECTIVE
  To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.
  
  
  METHODS
  Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.
  
  
  RESULTS
  Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.
  
  
  CONCLUSIONS
  PTEN silencing may be promising for spinal cord injury repair.
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Hengxing
authors.initials: H
authors.lastname: Zhou
authors.forename: Xueying
authors.initials: X
authors.lastname: Li
authors.affiliation: 3Department of Orthopaedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314 Anshanxi Road, Nankai District, Tianjin 300193, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Fuyuan
authors.initials: F
authors.lastname: Li
authors.forename: Zheng
authors.initials: Z
authors.lastname: Fu
authors.affiliation: 4School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: 4School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China.
authors.forename: Zhipin
authors.initials: Z
authors.lastname: Liang
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Tianci
authors.initials: T
authors.lastname: Chu
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Wang
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Ning
authors.affiliation: 4School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Kong
authors.affiliation: 1Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China.
authors.forename: Shiqing
authors.initials: S
authors.lastname: Feng
mesh_terms: D000818:Animals; D001369:Axons / Q000473:pathology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000473:pathology*; D002921:Cicatrix / Q000473:pathology; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006614:Hindlimb / Q000473:pathology / Q000503:physiopathology; D016086:Lentivirus / Q000378:metabolism; D009043:Motor Activity; D016501:Neurites / Q000378:metabolism* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D017208:Rats, Wistar; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology*; D013569:Synapses / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D051059:PTEN Phosphohydrolase
keywords: PTEN; RNA interference; axon growth/regeneration; functional recovery; spinal cord injury