title: Behavioral tests and oxidative stress evaluation in mitochondria isolated from the brain and liver of mice treated with riparin A.
abstract: OBJECTIVE
  This study aimed at evaluating the oxidative stress in mitochondria isolated from the brain and liver of mice treated with riparin A, as well as the locomotor activity and myorelaxant effect of this compound. Behavioral models of rota rod and open field tests were used for locomotor activity and myorelaxant effect evaluation.
  
  
  METHODS
  The animals were divided into five groups (n=8), which were treated with: diazepam (1mg/kg, i.p), riparin A (5, 10, and 20mg/kg, o.r.) or vehicle (0.9% saline, o.r.). The oxidative stress evaluation was carried out in mitochondria isolated from the brain and liver of mice from five experimental groups (n=8), which were treated with: ascorbic acid (250 mg/kg; positive control), vehicle (0.9% saline; negative control) and riparin A (5, 10 and 20mg/kg).
  
  
  RESULTS
  In an open field and rota rod test a significant difference in the number of crossings, in time of permanence on the swivel bar and in the number of falls in riparin A treated animals (5, 10 and 20mg/kg) was not observed, when compared with negative control (vehicle) (p>0.05). In comparison to the negative control, there was a reduction of lipid peroxidation levels and nitrite content in mice treated with riparin A (p<0.05). Reduced glutathione (GSH) levels (p<0.05), superoxide dismutase (SOD) and catalase activities increased in the brain (rip A 5mg/kg; p<0.05), while in the liver SOD remained unchanged (p>0.05) and catalase activity (p<0.05) was reduced.
  
  
  CONCLUSIONS
  Riparin A was presented as a bioactive molecule devoid of adverse effects of alteration of motor activity.
authors.affiliation: Laboratory for Research in Experimental Neurochemistry, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina, PI 64049550, Brazil. Electronic address: geandraenf@hotmail.com.
authors.forename: Geandra Batista Lima
authors.initials: GB
authors.lastname: Nunes
authors.affiliation: Laboratory for Research in Experimental Neurochemistry, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina, PI 64049550, Brazil. Electronic address: luciana.muratori@hotmail.com.
authors.forename: Luciana Muratori
authors.initials: LM
authors.lastname: Costa
authors.affiliation: Laboratory Chemistry of Bioactive Natural and Synthetic Products, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina, PI 64049550, Brazil. Electronic address: stanleychavez@yahoo.com.br.
authors.forename: Stanley Juan Chávez
authors.initials: SJ
authors.lastname: Gutierrez
authors.affiliation: Chemistry Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA. Electronic address: prabodhsatyal@gmail.com.
authors.forename: Prabodh
authors.initials: P
authors.lastname: Satyal
authors.affiliation: Laboratory for Research in Experimental Neurochemistry, Campus Ministro Petrônio Portella, Federal University of Piauí, Teresina, PI 64049550, Brazil. Electronic address: rivmendes@hotmail.com.
authors.forename: Rivelilson Mendes
authors.initials: RM
authors.lastname: de Freitas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D001549:Benzamides / Q000494:pharmacology*; D001923:Brain Chemistry / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009043:Motor Activity / Q000187:drug effects; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010627:Phenethylamines / Q000494:pharmacology*; D004856:Postural Balance / Q000187:drug effects
chemical_list: D000975:Antioxidants; D001549:Benzamides; D009125:Muscle Relaxants, Central; C000596368:N-phenethylbenzamide; D009573:Nitrites; D010627:Phenethylamines
keywords: Antioxidant; Free radicals; Open field test; Reactive oxygen species; Rota rod