title: Significance of algal polymer in designing amphotericin B nanoparticles.
abstract: Development of oral amphotericin B (AmB) loaded nanoparticles (NPs) demands a novel technique which reduces its toxicity and other associated problems. Packing of AmB in between two oppositely charged ions by polyelectrolyte complexation technique proved to be a successful strategy. We have developed a novel carrier system in form of polyelectrolyte complex of AmB by using chitosan (CS) and porphyran (POR) as two oppositely charged polymers with TPP as a crosslinking agent. Initially POR was isolated from Porphyra vietnamensis followed by the fact that its alkali induced safe reduction in molecular weight was achieved. Formulation was optimized using three-factor three-level (3(3)) central composite design. High concentration of POR in NPs was confirmed by sulfated polysaccharide (SP) assay. Degradation and dissolution studies suggested the stability of NPs over wide pH range. Hemolytic toxicity data suggested the safety of prepared formulation. In vivo and in vitro antifungal activity demonstrated the high antifungal potential of optimized formulation when compared with standard drug and marketed formulations. Throughout the study TPP addition did not cause any significant changes. Therefore, these experimental oral NPs may represent an interesting carrier system for the delivery of AmB.
authors.affiliation: PDMCOP, Bahadurgarh, Haryana 124507, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Bhatia
authors.affiliation: PDMCOP, Bahadurgarh, Haryana 124507, India.
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Department of Pharmaceutical Sciences, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Sharma
authors.affiliation: School of Pharmacy, Faculty of Applied Medical Sciences, Lovely Professional University, Phagwara, Punjab 144411, India.
authors.forename: Kalpana
authors.initials: K
authors.lastname: Nagpal
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India.
authors.forename: Tanmoy
authors.initials: T
authors.lastname: Bera
mesh_terms: D000284:Administration, Oral; D000666:Amphotericin B / Q000009:adverse effects / Q000737:chemistry* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D000935:Antifungal Agents / Q000737:chemistry / Q000627:therapeutic use; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D011108:Polymers / Q000737:chemistry / Q000627:therapeutic use; D012685:Sepharose / Q000031:analogs & derivatives / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D000935:Antifungal Agents; D004337:Drug Carriers; D011108:Polymers; C038549:porphyran; D000666:Amphotericin B; D012685:Sepharose; D048271:Chitosan