title: Plasmid transfection in mammalian cells spatiotemporally tracked by a gold nanoparticle.
abstract: Recent advances in cell transfection have suggested that delivery of a gene on a gold nanoparticle (AuNP) can enhance transfection efficiency. The mechanism of transfection is poorly understood, particularly when the gene is appended to a AuNP, as expression of the desired exogenous protein is dependent not only on the efficiency of the gene being taken into the cell but also on efficient endosomal escape and cellular processing of the nucleic acid. Design of a multicolor surface energy transfer (McSET) molecular beacon by independently dye labeling a linearized plasmid and short duplex DNA (sdDNA) appended to a AuNP allows spatiotemporal profiling of the transfection events, providing insight into package uptake, disassembly, and final plasmid expression. Delivery of the AuNP construct encapsulated in Lipofectamine2000 is monitored in Chinese hamster ovary cells using live-cell confocal microscopy. The McSET beacon signals the location and timing of the AuNP release and endosomal escape events for the plasmid and the sdDNA discretely, which are correlated with plasmid transcription by fluorescent protein expression within the cell. It is observed that delivery of the construct leads to endosomal release of the plasmid and sdDNA from the AuNP surface at different rates, prior to endosomal escape. Slow cytosolic diffusion of the nucleic acids is believed to be the limiting step for transfection, impacting the time-dependent expression of protein. The overall protein expression yield is enhanced when delivered on a AuNP, possibly due to better endosomal escape or lower degradation prior to endosomal escape.
authors.affiliation: Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306-4390, United States.
authors.forename: Megan E
authors.initials: ME
authors.lastname: Muroski
authors.forename: Kate J F
authors.initials: KJ
authors.lastname: Carnevale
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Riskowski
authors.forename: Geoffrey F
authors.initials: GF
authors.lastname: Strouse
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006046:Gold / Q000737:chemistry*; D042541:Intracellular Space / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D062211:Spatio-Temporal Analysis; D014162:Transfection
chemical_list: D004337:Drug Carriers; D006046:Gold; D004247:DNA
keywords: SET; energy transfer; gold nanoparticle; in vitro; molecular beacon; optical imaging; transfection