title: Bacterial production of short-chain organic acids and trehalose from levulinic acid: a potential cellulose-derived building block as a feedstock for microbial production.
abstract: Levulinic acid (LA) is a platform chemical derived from cellulosic biomass, and the expansion of LA utilization as a feedstock is important for production of a wide variety of chemicals. To investigate the potential of LA as a substrate for microbial conversion to chemicals, we isolated and identified LA-utilizing bacteria. Among the six isolated strains, Pseudomonas sp. LA18T and Rhodococcus hoagie LA6W degraded up to 70 g/L LA in a high-cell-density system. The maximal accumulation of acetic acid by strain LA18T and propionic acid by strain LA6W was 13.6 g/L and 9.1 g/L, respectively, after a 4-day incubation. Another isolate, Burkholderia stabilis LA20W, produced trehalose extracellularly in the presence of 40 g/L LA to approximately 2 g/L. These abilities to produce useful compounds supported the potential of microbial LA conversion for future development and cellulosic biomass utilization.
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. Electronic address: hiroshi.habe@aist.go.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Sato
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Morita
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Tokuma
authors.initials: T
authors.lastname: Fukuoka
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Kirimura
authors.affiliation: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
authors.forename: Dai
authors.initials: D
authors.lastname: Kitamoto
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D007656:Ketoglutaric Acids / Q000378:metabolism; D007982:Levulinic Acids / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D011422:Propionates / Q000378:metabolism; D013997:Time Factors; D014199:Trehalose / Q000096:biosynthesis*
chemical_list: D002264:Carboxylic Acids; D003470:Culture Media; D007656:Ketoglutaric Acids; D007982:Levulinic Acids; D011422:Propionates; D002482:Cellulose; D014199:Trehalose; C029658:propionic acid; D019342:Acetic Acid; C032246:levulinic acid
keywords: Cellulosic biomass utilization; Levulinic acid; Microbial production; Short-chain organic acid; Trehalose