title: The budding yeast Polo-like kinase Cdc5 is released from the nucleus during anaphase for timely mitotic exit.
abstract: Polo-like kinases are important regulators of multiple mitotic events; however, how Polo-like kinases are spatially and temporally regulated to perform their many tasks is not well understood. Here, we examined the subcellular localization of the budding yeast Polo-like kinase Cdc5 using a functional Cdc5-GFP protein expressed from the endogenous locus. In addition to the well-described localization of Cdc5 at the spindle pole bodies (SPBs) and the bud neck, we found that Cdc5-GFP accumulates in the nucleus in early mitosis but is released to the cytoplasm in late mitosis in a manner dependent on the Cdc14 phosphatase. This Cdc5 release from the nucleus is important for mitotic exit because artificial sequestration of Cdc5 in the nucleus by addition of a strong nuclear localization signal (NLS) resulted in mitotic exit defects. We identified a key cytoplasmic target of Cdc5 as Bfa1, an inhibitor of mitotic exit. Our study revealed a novel layer of Cdc5 regulation and suggests the existence of a possible coordination between Cdc5 and Cdc14 activity.
authors.affiliation: a Department of Biology and Rosenstiel Basc Medical Sciences Research Center ; Brandeis University ; Waltham , MA USA.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Botchkarev
authors.forename: Valentina
authors.initials: V
authors.lastname: Rossio
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D000705:Anaphase / Q000235:genetics / Q000502:physiology*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D004718:Saccharomycetales / Q000201:enzymology / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D017346:Protein Serine-Threonine Kinases
keywords: Bfa1; Cdc14; Cdc5; FEAR; FEAR, Cdc Fourteen Early Anaphase Release; MEN; MEN, Mitotic Exit Network; Polo-like kinase; mitosis; mitotic exit