title: Electrospun gelatin nanofibers: a facile cross-linking approach using oxidized sucrose.
abstract: Gelatin nanofibers were fabricated via electrospinning with minimal toxicity from solvents and cross-linking agents. Electrospinning was carried out using a solvent system based on water and acetic acid (8:2, v/v). Acetic acid concentration was kept as minimum as possible to reduce the toxic effects. Electrospun gelatin nanofibers were cross-linked with oxidized sucrose. Sucrose was oxidized by periodate oxidation to introduce aldehyde functionality. Cross-linking with oxidized sucrose could be achieved without compromising the nanofibrous architecture. Cross-linked gelatin nanofibers maintained the fibrous morphology even after keeping in contact with aqueous medium. The morphology of the cross-linked nanofibrous mats was examined by scanning electron microscopy (SEM). Oxidized sucrose cross-linked gelatin nanofibers exhibited improved thermal and mechanical properties. The nanofibrous mats were evaluated for cytotoxicity and cell viability using L-929 fibroblast cells. The results confirmed that oxidized sucrose cross-linked gelatin nanofibers were non-cytotoxic towards L-929 cells with good cell viability.
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology, Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: jalajak@iist.ac.in.
authors.forename: K
authors.initials: K
authors.lastname: Jalaja
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology, Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: nirmala@iist.ac.in.
authors.forename: Nirmala R
authors.initials: NR
authors.lastname: James
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D002470:Cell Survival; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D055595:Mechanical Phenomena; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D013395:Sucrose / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D013395:Sucrose; D005780:Gelatin
keywords: Gelatin; Nanofibers; Oxidized sucrose