title: Ammonia oxidizers in a pilot-scale multilayer rapid infiltration system for domestic wastewater treatment.
abstract: A pilot-scale multilayer rapid infiltration system (MRIS) for domestic wastewater treatment was established and efficient removal of ammonia and chemical oxygen demand (COD) was achieved in this study. The microbial community composition and abundance of ammonia oxidizers were investigated. Efficient biofilms of ammonia oxidizers in the stationary phase (packing material) was formed successfully in the MRIS without special inoculation. DGGE and phylogenetic analyses revealed that proteobacteria dominated in the MRIS. Relative abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) showed contrary tendency. In the flowing phase (water effluent), AOA diversity was significantly correlated with the concentration of dissolve oxygen (DO), NO3-N and NH3-N. AOB abundance was significantly correlated with the concentration of DO and chemical oxygen demand (COD). NH3-N and COD were identified as the key factors to shape AOB community structure, while no variable significantly correlated with that of AOA. AOA might play an important role in the MRIS. This study could reveal key environmental factors affecting the community composition and abundance of ammonia oxidizers in the MRIS.
authors.affiliation: School of Biological Science & Engineering, South China University of Technology, Guangzhou, 510006, China; Guangdong Institute of Microbiology, Guangzhou, 510070, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Lian
authors.affiliation: Guangdong Institute of Microbiology, Guangzhou, 510070, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Xu
authors.affiliation: Guangdong Institute of Microbiology, Guangzhou, 510070, China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China.
authors.forename: Fanrong
authors.initials: F
authors.lastname: Chen
authors.affiliation: Guangdong Institute of Microbiology, Guangzhou, 510070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Guo
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D058645:Denaturing Gradient Gel Electrophoresis; D005374:Filtration / Q000379:methods; D059013:Microbial Consortia / Q000235:genetics*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater
chemical_list: D000641:Ammonia; D012336:RNA, Ribosomal, 16S; D062065:Wastewater