title: Hydrogen sulfide inhalation decreases early blood-brain barrier permeability and brain edema induced by cardiac arrest and resuscitation.
abstract: The effects of hydrogen sulfide (H2S) on blood-brain barrier (BBB) and brain edema after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) remain poorly understood. We investigated the effects of exogenous 80-p.p.m. H2S gas on BBB, brain water content, neurologic outcome, and survival rate after CA and CPR. Cardiopulmonary resuscitation followed CA induced in rats by ventricular fibrillation for 6 minutes. Results show that inhalation of 80-p.p.m. H2S significantly reduced the permeability of the BBB in both in the cortex and hippocampus at 24 hours after resuscitation. Hydrogen sulfide also lessened brain edema in the cortex and hippocampus, ameliorated neurologic outcome as evaluated by neurologic deficit score and tape removal test, and improved the 14-day survival rate. Hydrogen sulfide also attenuated CA and CPR-induced increases of matrix metalloproteinase-9 (MMP-9) activity and vascular endothelial growth factor (VEGF) expression, and increased the expression of angiogenin-1 (Ang-1). These results indicate that inhalation of 80-p.p.m. H2S immediately after CPR attenuated BBB permeability and brain edema, and improved neurologic outcome and 14-day survival of rats after CA. The therapeutic benefits of H2S could be associated with suppression of MMP-9 and VEGF expression and increased expression of Ang-1.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Eerdunmutu
authors.initials: E
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Qier
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Third Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Hangbing
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Long
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D015153:Blotting, Western; D001929:Brain Edema / Q000209:etiology*; D002199:Capillary Permeability / Q000187:drug effects; D016887:Cardiopulmonary Resuscitation; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000150:complications* / Q000503:physiopathology; D006862:Hydrogen Sulfide / Q000008:administration & dosage*; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018696:Neuroprotective Agents; D006862:Hydrogen Sulfide