title: Expression, purification and X-ray crystallographic analysis of the Helicobacter pylori blood group antigen-binding adhesin BabA.
abstract: Helicobacter pylori is a human pathogen that colonizes about 50% of the world's population, causing chronic gastritis, duodenal ulcers and even gastric cancer. A steady emergence of multiple antibiotic resistant strains poses an important public health threat and there is an urgent requirement for alternative therapeutics. The blood group antigen-binding adhesin BabA mediates the intimate attachment to the host mucosa and forms a major candidate for novel vaccine and drug development. Here, the recombinant expression and crystallization of a soluble BabA truncation (BabA(25-460)) corresponding to the predicted extracellular adhesin domain of the protein are reported. X-ray diffraction data for nanobody-stabilized BabA(25-460) were collected to 2.25 Å resolution from a crystal that belonged to space group P21, with unit-cell parameters a = 50.96, b = 131.41, c = 123.40 Å, α = 90.0, β = 94.8, γ = 90.0°, and which was predicted to contain two BabA(25-460)-nanobody complexes per asymmetric unit.
authors.affiliation: Structural and Molecular Microbiology, VIB Department of Structural Biology, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Suresh
authors.initials: S
authors.lastname: Subedi
authors.affiliation: Structural and Molecular Microbiology, VIB Department of Structural Biology, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Kristof
authors.initials: K
authors.lastname: Moonens
authors.affiliation: Research Group Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Ema
authors.initials: E
authors.lastname: Romão
authors.affiliation: Structural and Molecular Microbiology, VIB Department of Structural Biology, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Alvin
authors.initials: A
authors.lastname: Lo
authors.affiliation: Structure and Function of Biological Membranes, Université Libre de Bruxelles, Boulevard du Triomphe, 1050 Brussels, Belgium.
authors.forename: Guy
authors.initials: G
authors.lastname: Vandenbussche
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, SE-901 87 Umeå, Sweden.
authors.forename: Jeanna
authors.initials: J
authors.lastname: Bugaytsova
authors.affiliation: Research Group Cellular and Molecular Immunology, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Serge
authors.initials: S
authors.lastname: Muyldermans
authors.affiliation: Department of Medical Biochemistry and Biophysics, Umeå University, SE-901 87 Umeå, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Borén
authors.affiliation: Structural and Molecular Microbiology, VIB Department of Structural Biology, VIB, Pleinlaan 2, 1050 Brussels, Belgium.
authors.forename: Han
authors.initials: H
authors.lastname: Remaut
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D001483:Base Sequence; D001789:Blood Group Antigens / Q000276:immunology*; D018360:Crystallography, X-Ray; D017931:DNA Primers; D016480:Helicobacter pylori / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; C400429:BabA protein, Helicobacter pylori; D001789:Blood Group Antigens; D017931:DNA Primers
keywords: BabA; Helicobacter pylori; adhesin; nanobody