title: Fast assessment of planar chromatographic layers quality using pulse thermovision method.
abstract: The main goal of this paper is to demonstrate capability of pulse thermovision (thermal-wave) methodology for sensitive detection of photothermal non-uniformities within light scattering and semi-transparent planar stationary phases. Successful visualization of stationary phases defects required signal processing protocols based on wavelet filtration, correlation analysis and k-means 3D segmentation. Such post-processing data handling approach allows extremely sensitive detection of thickness and structural changes within commercially available planar chromatographic layers. Particularly, a number of TLC and HPTLC stationary phases including silica, cellulose, aluminum oxide, polyamide and octadecylsilane coated with adsorbent layer ranging from 100 to 250μm were investigated. Presented detection protocol can be used as an efficient tool for fast screening the overall heterogeneity of any layered materials. Moreover, described procedure is very fast (few seconds including acquisition and data processing) and may be applied for fabrication processes online controlling. In spite of planar chromatographic plates this protocol can be used for assessment of different planar separation tools like paper based analytical devices or micro total analysis systems, consisted of organic and non-organic layers.
authors.affiliation: Chair of Multimedia Systems and Artificial Intelligence, Faculty of Electronics and Computer Sciences, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland.
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Suszyński
authors.affiliation: Chair of Multimedia Systems and Artificial Intelligence, Faculty of Electronics and Computer Sciences, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Świta
authors.affiliation: State Higher Vocational School in Koszalin, Leśna 1, 75-582 Koszalin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Loś
authors.affiliation: Apteka "Bursztynowa", Dworcowa 11/15, 75-201 Koszalin, Poland.
authors.forename: Magdalena B
authors.initials: MB
authors.lastname: Zarzycka
authors.affiliation: Section of Toxicology and Bioanalytics, Department of Civil and Environmental Engineering, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kaleniecka
authors.affiliation: Section of Toxicology and Bioanalytics, Department of Civil and Environmental Engineering, Koszalin University of Technology, Śniadeckich 2, 75-453 Koszalin, Poland. Electronic address: pkzarz@wp.pl.
authors.forename: Paweł K
authors.initials: PK
authors.lastname: Zarzycki
mesh_terms: D002482:Cellulose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D012822:Silicon Dioxide / Q000737:chemistry; D013696:Temperature; D013997:Time Factors
chemical_list: D012822:Silicon Dioxide; D002482:Cellulose
keywords: Active thermography; Chromatography; Planar stationary phases quality; Stationary phase defects; Thermal contrast; Thin-layers