title: Robust ridge regression estimators for nonlinear models with applications to high throughput screening assay data.
abstract: Nonlinear regression is often used to evaluate the toxicity of a chemical or a drug by fitting data from a dose-response study. Toxicologists and pharmacologists may draw a conclusion about whether a chemical is toxic by testing the significance of the estimated parameters. However, sometimes the null hypothesis cannot be rejected even though the fit is quite good. One possible reason for such cases is that the estimated standard errors of the parameter estimates are extremely large. In this paper, we propose robust ridge regression estimation procedures for nonlinear models to solve this problem. The asymptotic properties of the proposed estimators are investigated; in particular, their mean squared errors are derived. The performances of the proposed estimators are compared with several standard estimators using simulation studies. The proposed methodology is also illustrated using high throughput screening assay data obtained from the National Toxicology Program.
authors.affiliation: Department of Applied Statistics, Chung-Ang University, Seoul, Korea.
authors.forename: Changwon
authors.initials: C
authors.lastname: Lim
mesh_terms: D056808:Biostatistics / Q000379:methods; D003198:Computer Simulation; D056945:Hep G2 Cells; D057166:High-Throughput Screening Assays / Q000706:statistics & numerical data*; D006801:Humans; D015233:Models, Statistical; D017711:Nonlinear Dynamics; D010601:Pharmacology, Clinical / Q000706:statistics & numerical data; D012044:Regression Analysis; D018675:Toxicity Tests / Q000706:statistics & numerical data; D014116:Toxicology / Q000706:statistics & numerical data
keywords: HTS assay; M-estimation; dose-response; pharmacology; ridge regression; toxicology