title: Krebs cycle metabolon formation: metabolite concentration gradient enhanced compartmentation of sequential enzymes.
abstract: Dynamics of metabolon formation in mitochondria was probed by studying diffusional motion of two sequential Krebs cycle enzymes in a microfluidic channel. Enhanced directional co-diffusion of both enzymes against a substrate concentration gradient was observed in the presence of intermediate generation. This reveals a metabolite directed compartmentation of metabolic pathways.
authors.affiliation: Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, USA. minteer@chem.utah.edu.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.forename: Lindsey N
authors.initials: LN
authors.lastname: Pelster
authors.forename: Shelley D
authors.initials: SD
authors.lastname: Minteer
mesh_terms: D002952:Citric Acid Cycle; D004058:Diffusion; D004798:Enzymes / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D046210:Microfluidic Analytical Techniques; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D009243:NAD / Q000378:metabolism
chemical_list: D004798:Enzymes; D008293:Malates; D009243:NAD; C030298:malic acid