title: Two novel male-associated peroxinectin genes are downregulated by exposure to delousing drugs in Caligus rogercresseyi.
abstract: Peroxinectin (PX) is a protein involved in cell adhesion, peroxidase activities, and the encapsulation of invaders in diverse species, including parasitic copepods. Recently, a transcript denoted peroxinectin-like was identified in the salmon louse Lepeophtheirus salmonis, and this was significantly correlated with the immune response of host fish. Thus, the PX gene is a potential candidate to evaluate host-parasite interactions, as well as to analyze responses to delousing drugs used in the salmon aquaculture industry worldwide. The objective of this study was to identify Peroxinectin transcripts in the Chilean salmon louse Caligus rogercresseyi, and to determine expression levels after exposition to the antiparasitics deltamethrin and azamethiphos. Two novel transcript homologs to peroxinectins were identified from a transcriptomic library of C. rogercresseyi. Moreover, in silico gene transcription levels were evaluated through RNA-seq analyses based on unique gene read levels in transcriptomic libraries that were constructed from sea lice exposed to delousing drugs. The identified transcripts were named Peroxinectin-Cr1 and Peroxinectin-Cr2, which, respectively, had lengths of 2543 and 2555 base pairs. Both PX transcripts were highly associated with male adults, and transcription levels were significantly reduced by deltamethrin and azamethiphos. This result suggests a modulation of peroxinectin in response to delousing drugs.
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO Box 160-C, Concepcion, Chile.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Núñez-Acuña
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, PO Box 160-C, Concepcion, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D033342:Copepoda / Q000187:drug effects* / Q000235:genetics*; D015536:Down-Regulation; D005393:Fish Diseases / Q000469:parasitology*; D006790:Host-Parasite Interactions; D008297:Male; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D063086:Organothiophosphates / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology*; D012474:Salmon / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000977:Antiparasitic Agents; D015815:Cell Adhesion Molecules; D009570:Nitriles; D063086:Organothiophosphates; D011722:Pyrethrins; C017180:decamethrin; C072763:azamethiphos
keywords: Azamethiphos; Caligus rogercresseyi; Deltamethrin; Peroxinectin; RNA-seq