title: Differential interactions of two local anesthetics with phospholipid membrane and nonerythroid spectrin: Localization in presence of cholesterol and ganglioside, GM1.
abstract: Interactions of two local anesthetics, dibucaine and tetracaine have been studied with phospholipid vesicles containing cholesterol and/or monosialogangliosides (GM1) using fluorescence spectroscopy. The fluorescence intensity of tetracaine showed a marked increase with the increasing molar ratio of the phospholipid to tetracaine, while that of dibucaine showed opposite effects. Steady state anisotropy and the wavelength of maximum emission (λmax) decreased with the increasing phospholipids to tetracaine ratio. The extent of such changes in anisotropy and λmax in the presence and absence of two important components of neuronal membranes, cholesterol and GM1 indicated differential membrane localization of the two local anesthetics. To understand the intercellular mode of action of local anesthetics, we have also studied the interactions of dibucaine and tetracaine with brain spectrin which indicate differential spectrin interactions with similar binding strength. Thermodynamic parameters associated with such binding reveal that binding is favored by entropy. Tetracaine brings about distinct structural changes in spectrin compared to dibucaine, as reflected in the tryptophan mean lifetime and far-UV CD spectra. Tetracaine also exhibits a detergent-like property inducing concentration dependent decrease in spectrin anisotropy, further indicating structural changes in brain spectrin with probable implications in its anesthetic potential.
authors.affiliation: Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India. Electronic address: abhijit.chakrabarti@saha.ac.in.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
authors.affiliation: Chemistry Department, University of Calcutta 92, A. P. C Road, Kolkata 700009, India.
authors.forename: Malay
authors.initials: M
authors.lastname: Patra
mesh_terms: D000465:Algorithms; D000779:Anesthetics, Local / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D003992:Dibucaine / Q000737:chemistry / Q000378:metabolism*; D005677:G(M1) Ganglioside / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D008956:Models, Chemical; D008958:Models, Molecular; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012756:Sheep; D013049:Spectrin / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013748:Tetracaine / Q000737:chemistry / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D000779:Anesthetics, Local; D008563:Membrane Lipids; D010743:Phospholipids; D013748:Tetracaine; D013049:Spectrin; D005677:G(M1) Ganglioside; D002784:Cholesterol; D003992:Dibucaine
keywords: Brain spectrin; Cholesterol; Ganglioside; Local anesthetic; Phospholipid vesicle