title: Cross-protection between experimental anti-leptospirosis bacterins.
abstract: We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The challenge was performed with cross-strains of serogroup Pomona tested in accordance with international standards established for the potency test. After 21 days of the challenge, survivors were killed to evaluate the condition of Leptospira renal carrier. Experimental bacterins protected hamsters against homologous and heterologous strains, demonstrating the existence of cross-protection. The commercial vaccine protected the hamsters challenged with both strains, but there was a high proportion of animals diagnosed as renal carriers when the challenge was performed with strain GR6, isolated from pigs in Brazil.
authors.affiliation: Laboratório de Tuberculose Centro de Pesquisa e Desenvolvimento de Sanidade Animal Instituto Biológico São PauloSP Brazil Laboratório de Tuberculose, Centro de Pesquisa e Desenvolvimento de Sanidade Animal, Instituto Biológico, São Paulo, SP, Brazil.
authors.forename: Cristina Corsi
authors.initials: CC
authors.lastname: Dib
authors.affiliation: Laboratório de Zoonoses Bacterianas Departamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São PauloSP Brazil Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Amane Paldês
authors.initials: AP
authors.lastname: Gonçales
authors.affiliation: Laboratório de Zoonoses Bacterianas Departamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São PauloSP Brazil Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Zenaide Maria
authors.initials: ZM
authors.lastname: de Morais
authors.affiliation: Laboratório de Zoonoses Bacterianas Departamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São PauloSP Brazil Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Gisele Oliveira
authors.initials: GO
authors.lastname: de Souza
authors.affiliation: Laboratório de Zoonoses Bacterianas Departamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São PauloSP Brazil Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Miraglia
authors.affiliation: Laboratório de Bacteriologia Instituto Butantan São PauloSP Brazil Laboratório de Bacteriologia, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Patricia Antonia Estima
authors.initials: PA
authors.lastname: Abreu
authors.affiliation: Laboratório de Zoonoses Bacterianas Departamento de Medicina Veterinária Preventiva e Saúde Animal Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo São PauloSP Brazil Laboratório de Zoonoses Bacterianas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Silvio Arruda
authors.initials: SA
authors.lastname: Vasconcellos
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000536:Aluminum Hydroxide / Q000008:administration & dosage; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D002353:Carrier State / Q000382:microbiology / Q000517:prevention & control; D006224:Cricetinae; D056738:Cross Protection; D007668:Kidney / Q000382:microbiology; D007919:Leptospira / Q000302:isolation & purification; D007922:Leptospirosis / Q000276:immunology* / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D000276:Adjuvants, Immunologic; D001428:Bacterial Vaccines; D000536:Aluminum Hydroxide
keywords: Pomona; bacterin; cross-protection; hamsters