title: [Characteristic fingerprint analysis and determination of main components on Andrographis paniculata extract by UPLC].
abstract: OBJECTIVE
  To establish an analytical method for characteristic fingerprint and determination of main components of Andrographis paniculata Extract by UPLC.
  
  
  METHODS
  The chromatographic conditions were Waters ACQUITY UPLC BEH-C18 (2. 1 mm x 0 mm,1.7 μm)by gradient elute using acetonitrile-water as mobile phase(0 -2 min,20% ~ 25% A;2 ~ 5 min,25% ~ 35% A;5 ~ 7 min,35% A;7 ~10 min,35%~ 55% A) at a flow rate of 0. 5 min/mL,detecting wavelength at 220 nm. Results:Contents of the andrographolide, neoandrographolide, 14-deoxyandrographolide and 14-deoxy-l11,12-didehydroandrographolide had good resolution with the correlation coefficients exceed 0. 9999 and the average percent recovery lied in 97. 2% to 103.9%, RSD was less than 3.0% (n = 6). The chromatograms of Andrographis paniculata Extract shared seven common peaks in which four of them were recognized by reference standard with the similarities over 0. 9.
  
  
  CONCLUSIONS
  It is a fast,accurate and validated method,and can be useful in quality evaluations of Andrographis paniculata Extract.
authors.forename: Hui-ye
authors.initials: HY
authors.lastname: Zhang
authors.forename: Xiao-fei
authors.initials: XF
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: De-qin
authors.initials: DQ
authors.lastname: Wamg
authors.forename: Qiao-hua
authors.initials: QH
authors.lastname: Deng
mesh_terms: D035685:Andrographis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes; D005960:Glucosides; D013764:Tetrahydronaphthalenes
chemical_list: C114791:14-deoxyandrographolide; D004224:Diterpenes; D005960:Glucosides; D013764:Tetrahydronaphthalenes; C030419:andrographolide; C082497:neoandrographolide