title: The superoxide dismutases of Candida glabrata protect against oxidative damage and are required for lysine biosynthesis, DNA integrity and chronological life survival.
abstract: The fungal pathogen Candida glabrata has a well-defined oxidative stress response, is extremely resistant to oxidative stress and can survive inside phagocytic cells. In order to further our understanding of the oxidative stress response in C. glabrata, we characterized the superoxide dismutases (SODs) Cu,ZnSOD (Sod1) and MnSOD (Sod2). We found that Sod1 is the major contributor to total SOD activity and is present in cytoplasm, whereas Sod2 is a mitochondrial protein. Both SODs played a central role in the oxidative stress response but Sod1 was more important during fermentative growth and Sod2 during respiration and growth in non-fermentable carbon sources. Interestingly, C. glabrata cells lacking both SODs showed auxotrophy for lysine, a high rate of spontaneous mutation and reduced chronological lifespan. Thus, our study reveals that SODs play an important role in metabolism, lysine biosynthesis, DNA protection and aging in C. glabrata.
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Marcela
authors.initials: M
authors.lastname: Briones-Martin-Del-Campo
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Orta-Zavalza
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Israel
authors.initials: I
authors.lastname: Cañas-Villamar
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Gutiérrez-Escobedo
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Juárez-Cepeda
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Karina
authors.initials: K
authors.lastname: Robledo-Márquez
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Omar
authors.initials: O
authors.lastname: Arroyo-Helguera
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Irene
authors.initials: I
authors.lastname: Castaño
authors.affiliation: IPICYT, División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José, no. 2055, Col. Lomas 4a Sección, San Luis Potosí, San Luis Potosí 78216, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: De Las Peñas
mesh_terms: D041221:Candida glabrata / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D004271:DNA, Fungal / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000096:biosynthesis*; D050296:Microbial Viability; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism*; D000099156:Superoxide Dismutase 2
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D008239:Lysine; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2