title: The complete mitogenomes of six higher termite species reconstructed from metagenomic datasets (Cornitermes sp., Cubitermes ugandensis, Microcerotermes parvus, Nasutitermes corniger, Neocapritermes taracua, and Termes hospes).
abstract: We reconstructed the complete mitochondrial genomes of six higher termite species from metagenomic datasets of their isolated hindgut compartments. The sequencing reads were retrieved and assembled with the mitochondrial-baiting and iterative-mapping algorithm (MITObim), which yielded closed mitogenomes without additional finishing efforts (average coverage ranging from 2300- to 17,000-fold). The genomes ranged from 16.1 to 17.6 kbp in size and had G+C contents between 32 and 35 mol%; each contained the same 37 genes present also in the mitochondria of other termite species. Our study substantially increases the number of termite mitogenomes available for phylogenetic studies and offers a facile strategy for identifying host species in metagenomic studies of their associated microbiota.
authors.affiliation: a Department of Biogeochemistry , Max Planck Institute for Terrestrial Microbiology , Marburg , Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Dietrich
authors.affiliation: a Department of Biogeochemistry , Max Planck Institute for Terrestrial Microbiology , Marburg , Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Brune
mesh_terms: D000818:Animals; D001482:Base Composition; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D030541:Databases, Genetic; D041981:Gastrointestinal Tract / Q000378:metabolism; D054629:Genome, Mitochondrial; D020049:Isoptera / Q000145:classification / Q000235:genetics*; D016013:Likelihood Functions; D056186:Metagenomics; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA
keywords: Metagenome; mitogenome; termites