title: Expression microarray as a tool to identify differentially expressed genes in horses suffering from inflammatory airway disease.
abstract: BACKGROUND
  Inflammatory airway disease (IAD) affects performance and well-being of horses. Diagnosis is primarily reached by bronchoalveolar lavage (BAL) cytology which is invasive and requires sedation.
  
  
  OBJECTIVE
  The purpose of this study was to identify differential gene expression in peripheral blood of horses with IAD using species-specific expression microarrays.
  
  
  METHODS
  Equine gene expression microarrays were used to investigate global mRNA expression in circulating leukocytes from healthy, IAD-affected, and low-performing Standardbred and endurance horses.
  
  
  RESULTS
  Nine genes in Standardbred and 61 genes in endurance horses were significantly differentially regulated (P < .001). These genes were related to inflammation (eg, ALOX15B, PLA2G12B, and PENK), oxidant/antioxidant balance (eg, DUOXA2 and GSTO1-1), and stress (eg, V1aR, GRLF1, Homer-2, and MAOB). All these genes were up-regulated, except down-regulated Homer-2 and MAOB. DUOXA2, ALOX15B, PLA2G12B, MAOB, and GRLF1 expression was further validated by RT-qPCR. An increase in glutathione peroxidase (GPx) activity in heparinized whole blood of IAD-affected Standardbred (P = .0025) and endurance horses (P = .0028) also suggests a deregulation of the oxidant/antioxidant balance. There was good correlation (r = .7354) between BAL neutrophil percentage and whole blood GPx activity in all horses.
  
  
  CONCLUSIONS
  This study showed that circulating blood cell gene expression reflects inflammatory responses in tissues. Whether any of the genes have potential for diagnostic applications in the future remains to be investigated. Although not specific for IAD, whole blood GPx activity appears to be correlated with BAL neutrophil percentage. This finding should be further assessed by testing a larger number of horses.
authors.affiliation: Department for Functional Sciences, Faculty of Veterinary Medicine, University of Liege, Liege, Belgium.
authors.forename: Eve
authors.initials: E
authors.lastname: Ramery
authors.forename: Audrey
authors.initials: A
authors.lastname: Fraipont
authors.forename: Eric A
authors.initials: EA
authors.lastname: Richard
authors.forename: Tatiana
authors.initials: T
authors.lastname: Art
authors.forename: Dimitri
authors.initials: D
authors.lastname: Pirottin
authors.forename: Wouter
authors.initials: W
authors.lastname: van Delm
authors.forename: Fabrice
authors.initials: F
authors.lastname: Bureau
authors.forename: Pierre
authors.initials: P
authors.lastname: Lekeux
mesh_terms: D000818:Animals; D001980:Bronchi / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation / Q000276:immunology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006098:Granulocytes / Q000276:immunology; D006734:Horse Diseases / Q000276:immunology* / Q000378:metabolism; D006736:Horses; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*; D008264:Macrophages / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis / Q000662:veterinary; D018384:Oxidative Stress; D011446:Prospective Studies
chemical_list: D005979:Glutathione Peroxidase
keywords: Equine; glutathione peroxidase; inflammation; oxidative stress; respiratory