title: The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster.
abstract: Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological `toolkit' to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated.
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: Greg
authors.initials: G
authors.lastname: Mann
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: Jesko
authors.initials: J
authors.lastname: Koehnke
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Bent
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: Rachael
authors.initials: R
authors.lastname: Graham
authors.affiliation: Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland.
authors.forename: Wael
authors.initials: W
authors.lastname: Houssen
authors.affiliation: Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Meston Walk, Aberdeen AB24 3UE, Scotland.
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: Uli
authors.initials: U
authors.lastname: Schwarz-Linek
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 8ST, Scotland.
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D000595:Amino Acid Sequence; D003500:Cyclization; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
chemical_list: D010456:Peptides, Cyclic
keywords: PatG; RiPPs; cyanobactins; patellamides