title: Fertility of boar semen cryopreserved in extender supplemented with butylated hydroxytoluene.
abstract: The present study was to determine the effect of butylated hydroxytoluene (BHT) on quality and fertilizing ability of frozen-thawed boar semen. In the first experiment, five crossbreds of Polish Landrace and Large White boars (five ejaculates per boar) were frozen in 0.5 mL straws after dilution with lactose-egg yolk-glycerol extender supplemented with 0 (control), 0.5, 1.0, and 2.0 mM BHT. The sperm quality was verified based on the motility (computer-assisted sperm analysis; total motility, %; progressive motility, %), membrane integrity (YO-PRO-1/propidium iodide [PI] assay), acrosome integrity (fluorescein isothiocyanate-conjugated with peanut agglutinin/PI), and lipid peroxidation (chemiluminescence method) at 15 minutes postthaw. In the second experiment, the semen cryopreserved in extender supplemented with 1.0 and 2.0 mM BHT were selected for intrauterine artificial insemination of synchronized gilts. An intrauterine artificial insemination with low numbers of spermatozoa (500 × 10(6)) was surgically infused into each uterine horn. The highest (P < 0.001) progressive motility (%), membrane integrity, and acrosomal integrity were noted by the addition of 1.0 and 2.0 mM BHT to the freezing extender. Moreover, the various concentrations (0.5-2.0 mM) of BHT caused a considerable decrease in lipid peroxidation in relation to the control extender (P < 0.001). The highest reproductive performance of inseminated gilts (farrowing rate, 86.7%; litter size, 10.8 ± 1.6) was observed when semen was cryopreserved in extender supplemented with 1.0 mM BHT. These findings demonstrate that the addition of 1.0 mM BHT to the freezing extender efficiently improves the fertilizing ability of postthaw boar spermatozoa.
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Kraków, Poland. Electronic address: monika.trzcinska@izoo.krakow.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Trzcińska
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Kraków, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Bryła
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Kraków, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gajda
authors.affiliation: National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Kraków, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Gogol
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D015925:Cryopreservation / Q000379:methods*; D005298:Fertility; D007091:Image Processing, Computer-Assisted; D007315:Insemination, Artificial / Q000662:veterinary; D015227:Lipid Peroxidation; D008297:Male; D012661:Semen / Q000187:drug effects / Q000502:physiology*; D055101:Semen Analysis / Q000662:veterinary*; D013081:Sperm Motility; D013552:Swine / Q000502:physiology*
chemical_list: D002084:Butylated Hydroxytoluene
keywords: Boar; Butylated hydroxytoluene; Frozen-thawed semen; Intrauterine artificial insemination; Reproductive performance