title: Physiological and molecular functions of the cytosolic peptide:N-glycanase.
abstract: Peptide:N-glycanase (PNGase) is a deglycosylating enzyme that acts on N-glycoproteins. A growing evidence exists to indicate that the cytosolic form of PNGase, which is ubiquitously distributed throughout eukaryotes, is not only implicated in the efficient degradation of misfolded glycoproteins destined for the proteasomal degradation but also in the generation of free oligosaccharides as the initial step in the non-lysosomal catabolism of N-glycans. This article summarizes the current state of our knowledge of the physiological and molecular functions of the cytosolic PNGase in a model organism, Saccharomyces cerevisiae, and also discusses the functional/structural diversities of this molecule within eukaryotes.
authors.affiliation: Glycometabolome Team, System Glycobiology Research Group, RIKEN-Max Planck Joint Center for System Chemical Biology, Global Research Cluster, RIKEN, Hirosawa 2-1, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Hirayama
authors.affiliation: Glycometabolome Team, System Glycobiology Research Group, RIKEN-Max Planck Joint Center for System Chemical Biology, Global Research Cluster, RIKEN, Hirosawa 2-1, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Hosomi
authors.affiliation: Glycometabolome Team, System Glycobiology Research Group, RIKEN-Max Planck Joint Center for System Chemical Biology, Global Research Cluster, RIKEN, Hirosawa 2-1, Japan. Electronic address: tsuzuki_gm@riken.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D003600:Cytosol / Q000201:enzymology*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D009844:Oligosaccharides / Q000378:metabolism; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000235:genetics / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D046988:Proteasome Endopeptidase Complex; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
keywords: Endoplasmic reticulum-associated degradation; Free oligosaccharides; N-glycosylation; Peptide:N-glycanase; Rad23; Ubiquitin–proteasome system