title: The effects of dopamine on antioxidant enzymes activities and reactive oxygen species levels in soybean roots.
abstract: In the current work, we investigated the effects of dopamine, an neurotransmitter found in several plant species on antioxidant enzyme activities and ROS in soybean (Glycine max L. Merrill) roots. The effects of dopamine on SOD, CAT and POD activities, as well as H2O2, O2(•-), melanin contents and lipid peroxidation were evaluated. Three-day-old seedlings were cultivated in half-strength Hoagland nutrient solution (pH 6.0), without or with 0.1 to 1.0 mM dopamine, in a growth chamber (25°C, 12 h photoperiod, irradiance of 280 μmol m(-2) s(-1)) for 24 h. Significant increases in melanin content were observed. The levels of ROS and lipid peroxidation decreased at all concentrations of dopamine tested. The SOD activity increased significantly under the action of dopamine, while CT activity was inhibited and POD activity was unaffected. The results suggest a close relationship between a possible antioxidant activity of dopamine and melanin and activation of SOD, reducing the levels of ROS and damage on membranes of soybean roots.
authors.affiliation: a Laboratory of Plant Biochemistry; Department of Biochemistry ; State University of Maringá ; Maringá , PR , Brazil.
authors.forename: Bruno Ribeiro
authors.initials: BR
authors.lastname: Gomes
authors.forename: Rita de Cássia
authors.initials: Rde C
authors.lastname: Siqueira-Soares
authors.forename: Wanderley Dantas
authors.initials: WD
authors.lastname: Dos Santos
authors.forename: Rogério
authors.initials: R
authors.lastname: Marchiosi
authors.forename: Anderson Ricardo
authors.initials: AR
authors.lastname: Soares
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Ferrarese-Filho
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D004298:Dopamine / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008543:Melanins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013025:Glycine max / Q000187:drug effects / Q000201:enzymology*; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008543:Melanins; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; D004298:Dopamine
keywords: CAT, catalase; H2O2, hydrogen peroxide; HO−, hydroxyl radical; L-DOPA, L-3,4-dihydroxyphenylalanine.; O2•−, superoxide anion; POD, peroxidase; ROS, reactive oxygen species; SOD, Superoxide dismutase; antioxidant enzymes; dopamine; melanin; plants; reactive oxygen species; soybean