title: Ginsenoside Rg1 and Rb1, in combination with salvianolic acid B, play different roles in myocardial infarction in rats.
abstract: BACKGROUND
  The herb pair of Salvia miltiorrhiza and Panax notoginseng has widely been used for improving coronary and cerebral circulation in China. However, the exact contribution of the major active components of S. miltiorrhiza and P. notoginseng to cardioprotection is far from clear. In the present study, three representative ingredients, salvianolic acid B (SalB) from S. miltiorrhiza and ginsenoside Rg1 (Rg1) and ginsenoside Rb1 (Rb1) from P. notoginseng, were selected to elucidate the mechanism of the herb pair at the ingredient level.
  
  
  METHODS
  The purity of SalB, Rg1, and Rb1 was >99%, as detected by high-performance liquid chromatography. Acute myocardial infarction was introduced by ligation of the left anterior descending coronary artery near the main pulmonary artery. Cardiac contractility was detected through a Mikro-tipped catheter, and cardiac infarct size was determined using triphenyltetrazolium chloride stain.
  
  
  RESULTS
  The combination of SalB and Rg1, and not the combination of SalB and Rb1, improved heart contractility in rats with myocardial infarction. The different contributions of Rg1 and Rb1, in combination with SalB, to cardioprotection provides further direction to optimize and modernize the herbal medicines containing S. miltiorrhiza and P. notoginseng.
  
  
  CONCLUSIONS
  The combination of SalB and Rg1 may provide potential protection against myocardial infarction.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Fukang
authors.initials: F
authors.lastname: Teng
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Li
authors.affiliation: Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Takarazuka University of Medical and Health Care, Hanayashiki-Midorigaoka, Takarazuka City, Japan.
authors.forename: Kenka
authors.initials: K
authors.lastname: Cho
authors.affiliation: Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xu
authors.affiliation: Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Wanying
authors.initials: W
authors.lastname: Wu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. Electronic address: jiangbh@simm.ac.cn.
authors.forename: Baohong
authors.initials: B
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D004338:Drug Combinations; D036145:Ginsenosides / Q000008:administration & dosage*; D008297:Male; D009203:Myocardial Infarction / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D053630:Depsides
chemical_list: D001572:Benzofurans; D002316:Cardiotonic Agents; D004338:Drug Combinations; D036145:Ginsenosides; C442759:ginsenoside Rb1; C076944:salvianolic acid B; D053630:Depsides; C035054:ginsenoside Rg1
keywords: Panax notoginseng; Salvia miltiorrhiza; acute myocardial infarction; ginsenoside Rb1; ginsenoside Rg1; salvianolic acid B