title: Ultrasensitive electrochemical immunosensor for squamous cell carcinoma antigen detection using lamellar montmorillonite-gold nanostructures as signal amplification.
abstract: Sodium montmorillonites (Na-Mont), which could be transformed from nano-montmorillonites, have large surface area, chemical stability, nontoxicity, high cation exchange property and superior adsorption ability. In this paper, Na-Mont were used as a support of polyaniline (PANI) and gold nanoparticles (AuNPs) via the interaction of aniline and HAuCl4 solution. A sandwich-type electrochemical immunosensor was developed to detect squamous cell carcinoma antigen (SCC-Ag). It used nitrogen-doped graphene sheets (N-GS) for the immobilization of primary anti-SCC antibodies (Ab1) and the combined Na-Mont-PANI-AuNPs nanocomposites as labels. Na-Mont-PANI-AuNPs have excellent catalytic ability towards the reduction of H2O2, thus enhance the sensitivity of the immunosensor. The immunosensor exhibits a wide linear range (1 pg/mL-5 ng/mL), a low detection limit (0.3 pg/mL), good reproducibility, selectivity and stability. This new type of immunosensor with Na-Mont-PANI-AuNPs as labels may provide potential application for the detection of SCC-Ag.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Jia
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Picheng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000097:blood*; D001546:Bentonite / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007118:Immunoassay; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D015843:Serpins / Q000097:blood*
chemical_list: D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D015843:Serpins; C051072:squamous cell carcinoma-related antigen; D001546:Bentonite; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Electrochemical immunosensor; Lamellar montmorillonite-gold nanostructures; Nitrogen-doped graphene sheets; Squamous cell carcinoma antigen