title: Complementary and synergistic activities of anti-V3, CD4bs and CD4i antibodies derived from a single individual can cover a wide range of HIV-1 strains.
abstract: Antibodies with modest neutralizing activity and narrow breadth are commonly elicited in HIV-1. Here, we evaluated the complementary and synergistic activities of a set of monoclonal antibodies (MAb) isolated from a single patient, directed to V3, CD4 binding site (CD4bs), and CD4 induced (CD4i) epitopes. Despite low somatic hypermutation percentages in the variable regions, these MAbs covered viral strains from subtypes B, C, A and CRF01_AE and transmitted/founder viruses in terms of binding, neutralizing and antibody-dependent cell-mediated cytotoxicity (ADCC) activities. In addition, a combination of the anti-V3 and CD4bs MAbs showed a synergistic effect over the neutralization of HIV-1JR-FL. A humoral response from a single patient covered a wide range of viruses by complementary and synergistic activities of antibodies with different specificities. Inducing a set of narrow neutralizing antibodies, easier to induce than the broadly neutralizing antibodies, could be a strategy for developing an effective vaccine against HIV-1.
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Kristel Paola
authors.initials: KP
authors.lastname: Ramirez Valdez
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Takeo
authors.initials: T
authors.lastname: Kuwata
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Maruta
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
authors.forename: Muntasir
authors.initials: M
authors.lastname: Alam
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan; AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yoshimura
authors.affiliation: Matsushita Project Laboratory, Center for AIDS Research, Kumamoto University, Kumamoto, Japan. Electronic address: shuzo@kumamoto-u.ac.jp.
authors.forename: Shuzo
authors.initials: S
authors.lastname: Matsushita
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D057134:Antibodies, Neutralizing / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D001666:Binding Sites, Antibody / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D015483:HIV Antibodies / Q000276:immunology* / Q000502:physiology; D015699:HIV Envelope Protein gp120 / Q000276:immunology* / Q000502:physiology; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000145:classification* / Q000276:immunology*; D006801:Humans; D010446:Peptide Fragments / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015704:CD4 Antigens; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; C514793:gp120 protein, Human immunodeficiency virus 1
keywords: ADCC activity; Conventional antibodies; HIV-1; Neutralizing antibodies; Synergy