title: High-resolution N(6) -methyladenosine (m(6) A) map using photo-crosslinking-assisted m(6) A sequencing.
abstract: N(6) -methyladenosine (m(6) A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m(6) A modification remain limited. We present here a photo-crosslinking-assisted m(6) A sequencing strategy (PA-m(6) A-seq) to more accurately define sites with m(6) A modification. Using this strategy, we obtained a high-resolution map of m(6) A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m(6) A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA-binding proteins.
authors.affiliation: Department of Chemistry, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60637 (USA) http://he-group.uchicago.edu.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.forename: Zhike
authors.initials: Z
authors.lastname: Lu
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.forename: Ye
authors.initials: Y
authors.lastname: Fu
authors.forename: Guan-Zheng
authors.initials: GZ
authors.lastname: Luo
authors.forename: Nian
authors.initials: N
authors.lastname: Liu
authors.forename: Dali
authors.initials: D
authors.lastname: Han
authors.forename: Dan
authors.initials: D
authors.lastname: Dominissini
authors.forename: Qing
authors.initials: Q
authors.lastname: Dai
authors.forename: Tao
authors.initials: T
authors.lastname: Pan
authors.forename: Chuan
authors.initials: C
authors.lastname: He
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology; D000906:Antibodies / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013890:Thiourea / Q000737:chemistry / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D000906:Antibodies; D016601:RNA-Binding Proteins; C073276:N(6)-methoxyadenine; D012313:RNA; D013890:Thiourea; D000225:Adenine
keywords: N6-methyladenosine; RNA modification; photo-crosslinking; transcriptome sequencing