title: Cytokine gene expression profiles in chicken spleen and intestinal tissues during Ascaridia galli infection.
abstract: In the poultry production industry, chickens with access to outdoor areas are exposed to a wide range of parasites e.g. the helminth Ascaridia galli. By real-time quantitative RT-PCR, the relative gene expression of the T helper 1 (Th1) cytokine IFN-γ, the T helper 2 (Th2) cytokine IL-13, the anti-inflammatory cytokines IL-10 and TGF-β4 and the pro-inflammatory cytokine IL-17F were determined over a period of 3 weeks in A. galli and non-A. galli-infected chickens. A characteristic Th2 response was observed in the jejunum of A. galli-infected chickens with increased expression of IL-13 and decreased expression of IFN-γ from day 14 post infection. At the putative time of larvae invasion into the intestinal mucosa (day 7), an increased expression of IFN-γ, IL-10, and TGF-β4 was observed in the spleen. At the putative onset of the innate immune response (day 10), a decreased expression of jejunal IFN-γ and IL-13 was observed. Finally, at the expected period of an adaptive immune response (days 14-21) a general decreased expression of IFN-γ and TGF-β4 in spleen and IFN-γ in jejunum was followed by a decreased expression of IFN-γ and IL-10 at day 21 in caecal tonsils.
authors.forename: Janne A
authors.initials: JA
authors.lastname: Pleidrup
authors.forename: Liselotte R
authors.initials: LR
authors.lastname: Norup
authors.forename: Tina S
authors.initials: TS
authors.lastname: Dalgaard
authors.forename: Pete
authors.initials: P
authors.lastname: Kaiser
authors.forename: Anders
authors.initials: A
authors.lastname: Permin
authors.forename: Torben W
authors.initials: TW
authors.lastname: Schou
authors.forename: Dorte Fink
authors.initials: DF
authors.lastname: Vadekær
authors.forename: Gregers
authors.initials: G
authors.lastname: Jungersen
authors.forename: Poul
authors.initials: P
authors.lastname: Sørensen
authors.forename: Helle R
authors.initials: HR
authors.lastname: Juul-Madsen
mesh_terms: D000818:Animals; D001197:Ascaridia / Q000276:immunology*; D002645:Chickens / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D005260:Female; D007422:Intestines / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology* / Q000469:parasitology; D013154:Spleen / Q000276:immunology; D059467:Transcriptome
chemical_list: D016207:Cytokines