title: Combined effects of alternariols mixture on human colon carcinoma cells.
abstract: Mycotoxins are naturally occurring contaminants encountered at high levels in a wide variety of agricultural products intended for human and animal consumptions. Various Alternaria mycotoxins may occur simultaneously in small grain cereals. Considering the concomitant production of alternariol (AOH) and alternariol monomethyl ether (AME), it is expected that humans and animals are exposed to the mixture rather than to individual compounds. Therefore, we studied the interactive effects of binary mixture of alternariols (AOH and AME) on the human intestinal cell line, HCT116 cells. Exposure of HCT116 cells to low cytotoxic alternariols doses, resulted in a moderate cytotoxicity manifested by a loss in the cell viability mediated by an activation of the mitochondrial apoptotic process, associated with the opening of mitochondrial permeability transition pore (PTP) and the loss of the mitochondrial transmembrane potential (ΔΨm). However, when combined, they exert a significant increase in their toxic potential. Altogether, our study showed that AOH and AME combination is obviously additive.
authors.affiliation: Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry , Rue Avicenne, Monastir , Tunisia .
authors.forename: Fatma
authors.initials: F
authors.lastname: Bensassi
authors.forename: Cindy
authors.initials: C
authors.lastname: Gallerne
authors.forename: Ossama
authors.initials: O
authors.lastname: Sharaf  el dein
authors.forename: Mohamed Rabeh
authors.initials: MR
authors.lastname: Hajlaoui
authors.forename: Hassen
authors.initials: H
authors.lastname: Bacha
authors.forename: Christophe
authors.initials: C
authors.lastname: Lemaire
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D045325:HCT116 Cells; D006801:Humans; D007783:Lactones / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D018570:Risk Assessment; D013997:Time Factors
chemical_list: D007783:Lactones; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D017382:Reactive Oxygen Species; C005197:alternariol; C018206:alternariol monomethyl ether
keywords: AME; AOH; Addition; HCT116; apoptosis