title: Melatonin-induced temporal up-regulation of gene expression related to ubiquitin/proteasome system (UPS) in the human malaria parasite Plasmodium falciparum.
abstract: There is an increasing understanding that melatonin and the ubiquitin/ proteasome system (UPS) interact to regulate multiple cellular functions. Post-translational modifications such as ubiquitination are important modulators of signaling processes, cell cycle and many other cellular functions. Previously, we reported a melatonin-induced upregulation of gene expression related to ubiquitin/proteasome system (UPS) in Plasmodium falciparum, the human malaria parasite, and that P. falciparum protein kinase 7 influences this process. This implies a role of melatonin, an indolamine, in modulating intraerythrocytic development of the parasite. In this report we demonstrate by qPCR analysis, that melatonin induces gene upregulation in nine out of fourteen genes of the UPS, consisting of the same set of genes previously reported, between 4 to 5 h after melatonin treatment. We demonstrate that melatonin causes a temporally controlled gene expression of UPS members.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São, São Paulo 05508-900, Brazil. fernandakoyama@gmail.com.
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Koyama
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil. maurousp@gmail.com.
authors.forename: Mauro F
authors.initials: MF
authors.lastname: Azevedo
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil. alexandre.budu@gmail.com.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Budu
authors.affiliation: Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32826, USA. dchak@ucf.edu.
authors.forename: Debopam
authors.initials: D
authors.lastname: Chakrabarti
authors.affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo 05508-090, Brazil. cgarcia@usp.br.
authors.forename: Célia R S
authors.initials: CR
authors.lastname: Garcia
mesh_terms: D000818:Animals; D006801:Humans; D008288:Malaria / Q000469:parasitology*; D008550:Melatonin / Q000494:pharmacology*; D010271:Parasites / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D025801:Ubiquitin / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
chemical_list: D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex; D008550:Melatonin