title: Diet with diphenyl diselenide mitigates quinclorac toxicity in silver catfish (Rhamdia quelen).
abstract: In this study, the protective effects of diphenyl diselenide [(PhSe)2] on quinclorac- induced toxicity were investigated in silver catfish (Rhamdia quelen). The fish were fed for 60 days with a diet in the absence or in the presence of 3.0 mg/Kg (PhSe)2. Animals were further exposed to 1 mg/L quinclorac for 8 days. At the end of experimental period, fish were euthanized and biopsies from liver and gills, as well as blood samples, were collected. The cortisol and metabolic parameters were determined in plasma, and those enzyme activities related to osmoregulation were assayed in the gills. In liver, some important enzyme activities of the intermediary metabolism and oxidative stress-related parameters, such as thiobarbituric acid-reactive substance (TBARS), protein carbonyl, catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), nonprotein thiols (NPSH) and ascorbic acid contents were also evaluated. Compared to the control group, quinclorac exposure significantly decreased hepatosomatic index and increased cortisol and lactate values in plasma. Moreover, the activities of fructose biphosphatase (FBPase), glucose-6-phosphate dehydrogenase (G6Pase), glycogen phosphorilase (GPase) and aspartate aminotransferase (AST) were significantly increased in liver. Quinclorac also induced lipid peroxidation while the activity of SOD, NPSH and ascorbic acid levels decreased in the liver. However, dietary (PhSe)2 reduced the herbicide-induced effects on the studied parameters. In conclusion, (PhSe)2 has beneficial properties based on its ability to attenuate toxicity induced by quinclorac by regulating energy metabolism and oxidative stress-related parameters.
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Charlene
authors.initials: C
authors.lastname: Menezes
authors.affiliation: Departamento de Biología, Facultad de Ciencias Del Mar y Ambientales, Campus de Excelencia International del Mar (CEI-MAR), Universidad de Cádiz Puerto Real, Cádiz, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Ruiz-Jarabo
authors.affiliation: Departamento de Biología, Facultad de Ciencias Del Mar y Ambientales, Campus de Excelencia International del Mar (CEI-MAR), Universidad de Cádiz Puerto Real, Cádiz, Spain; Instituto de Ciencias Marinas de Andalucía, Consejo Superior de Investigaciones Científicas (ICMAN-CSIC), Puerto Real, Cádiz, Spain.
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Martos-Sitcha
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Jossiele
authors.initials: J
authors.lastname: Leitemperger
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
authors.affiliation: Departamento de Biología, Facultad de Ciencias Del Mar y Ambientales, Campus de Excelencia International del Mar (CEI-MAR), Universidad de Cádiz Puerto Real, Cádiz, Spain.
authors.forename: Juan Miguel
authors.initials: JM
authors.lastname: Mancera
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Denis Broock
authors.initials: DB
authors.lastname: Rosemberg
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.
authors.forename: Vania Lucia
authors.initials: VL
authors.lastname: Loro
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D001555:Benzene Derivatives / Q000008:administration & dosage*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D002397:Catfishes / Q000378:metabolism*; D004032:Diet; D019587:Dietary Supplements; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D016566:Organoselenium Compounds / Q000008:administration & dosage*; D018384:Oxidative Stress; D020011:Protective Agents / Q000008:administration & dosage*; D011804:Quinolines / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D029941:Fish Proteins; D006540:Herbicides; D016566:Organoselenium Compounds; D020011:Protective Agents; D011804:Quinolines; D017392:Thiobarbituric Acid Reactive Substances; C061132:diphenyldiselenide; C489048:quinclorac; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D001205:Ascorbic Acid