title: On-line biosensor for the detection of putative toxicity in water contaminants.
abstract: Potential threat on drinking water requires monitoring solutions, such as the one proposed herein, as a real-time, wide ranged, water monitoring system to detect the presence of toxicants in water. We studied the role of a selected number of parameters affecting performance and, thus, improved the prototype into an optimized next-generation device, resulting in enabling increased measurement duration, coupled with increased sensitivity. The chosen parameters in question were the peristaltic flow system, the fiber probe matrix stability through a re-design of the fiber probe holder and flow unit cell, as well as the modulation of bacterial medium concentration to increase bioreporter performance while keeping biofouling in check. Measurements were made with spiked samples and validated with polluted field-collected samples.
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; School of Material Science and Engineering, Nanyang Technology University, Nanyang Avenue, 639798, Singapore; CREATE, Singapore.
authors.forename: Evgeni
authors.initials: E
authors.lastname: Eltzov
authors.affiliation: Department of Biotechnology Engineering, Faculty of Engineering Science, Sami Shimon, Ashdod, Israel.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Slobodnik
authors.affiliation: Laboratoire de Nanotechnologie et d'Instrumentation Optique, Institute Charles Delaunay, Université de technologie de Troyes, UMR CNRS 6281, 12 rue Marie-Curie BP 42060 10004 TROYES, Cedex, France.
authors.forename: Rodica E
authors.initials: RE
authors.lastname: Ionescu
authors.affiliation: Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; School of Material Science and Engineering, Nanyang Technology University, Nanyang Avenue, 639798, Singapore; CREATE, Singapore; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; The Ilse Katz, Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel. Electronic address: rsmarks@bgu.ac.il.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
mesh_terms: D015374:Biosensing Techniques; D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D002733:Chlorophenols / Q000032:analysis; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000032:analysis; D005336:Fiber Optic Technology / Q000295:instrumentation / Q000379:methods; D005618:Fresh Water / Q000737:chemistry*; D015870:Gene Expression; D017930:Genes, Reporter; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D057446:Hydrodynamics; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000295:instrumentation; D009876:Operon; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D002733:Chlorophenols; D029968:Escherichia coli Proteins; C489400:GrpE protein, E coli; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D014874:Water Pollutants, Chemical; C029107:4-chlorophenol; D000431:Ethanol; D008156:Luciferases
keywords: Bioluminescence; Flow-through monitoring; Sensor; Water pollution