title: Tropoelastin incorporation into a dermal regeneration template promotes wound angiogenesis.
abstract: Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post-surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization.
authors.affiliation: Burns Research Group, ANZAC Research Institute, University of Sydney, Concord, NSW, 2139, Australia.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Mithieux
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kong
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Wang
authors.forename: Cassandra
authors.initials: C
authors.lastname: Chong
authors.forename: Ali
authors.initials: A
authors.lastname: Fathi
authors.forename: Fariba
authors.initials: F
authors.lastname: Dehghani
authors.forename: Eleni
authors.initials: E
authors.lastname: Panas
authors.forename: John
authors.initials: J
authors.lastname: Kemnitzer
authors.forename: Robert
authors.initials: R
authors.lastname: Daniels
authors.forename: Roy M
authors.initials: RM
authors.lastname: Kimble
authors.forename: Peter K
authors.initials: PK
authors.lastname: Maitz
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Anthony S
authors.initials: AS
authors.lastname: Weiss
mesh_terms: D000818:Animals; D001706:Biopsy; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D020405:Dermis / Q000098:blood supply* / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D012038:Regeneration / Q000187:drug effects*; D016038:Skin Transplantation; D013314:Stress, Mechanical; D034421:Sus scrofa; D014333:Tropoelastin / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D014333:Tropoelastin
keywords: dermal substitute; integra; skin; tropoelastin; wound healing