title: Purified IgG from patients with obstetric but not IgG from non-obstetric antiphospholipid syndrome inhibit trophoblast invasion.
abstract: OBJECTIVE
  Some patients with antiphospholipid syndrome (APS) suffer pregnancy morbidity (PM) but not vascular thrombosis (VT), whilst others suffer VT only. Therefore, we compared the effects of IgG from VT+/PM- and VT-/PM+ subjects on human first-trimester trophoblast (HTR8) cells.
  
  
  METHODS
  HTR-8 cells were incubated with APS VT+/PM-, APS VT-/PM+ or healthy control (HC) IgG. We measured trophoblast invasion by cell invasion assay; mRNA expression of TLR4 and adaptor proteins; phosphorylation of p38 MAPK, NFκB and ERK; and expression of interleukin (IL)-8 and IL-6.
  
  
  RESULTS
  VT-/PM+ IgG, but not VT+/PM- IgG significantly reduced HTR-8 invasion. The effects on invasion were blocked by TLR-4 inhibition. Neither VT+/PM- nor VT-/PM+ IgG altered MyD88 mRNA expression, phosphorylation of signalling molecules or cytokine expression.
  
  
  CONCLUSIONS
  VT-/PM+ IgG exert functionally relevant effects on human trophoblast cells but VT+/PM- IgG do not.
authors.affiliation: Division of Medicine, Centre for Rheumatology, Rayne Institute, University College London (UCL), London, UK.
authors.forename: Katie
authors.initials: K
authors.lastname: Poulton
authors.forename: Vera M
authors.initials: VM
authors.lastname: Ripoll
authors.forename: Charis
authors.initials: C
authors.lastname: Pericleous
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Meroni
authors.forename: Maria
authors.initials: M
authors.lastname: Gerosa
authors.forename: Yiannis
authors.initials: Y
authors.lastname: Ioannou
authors.forename: Anisur
authors.initials: A
authors.lastname: Rahman
authors.forename: Ian P
authors.initials: IP
authors.lastname: Giles
mesh_terms: D000328:Adult; D017152:Antibodies, Antiphospholipid / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood / Q000276:immunology* / Q000473:pathology; D014327:Trophoblasts / Q000276:immunology* / Q000473:pathology
chemical_list: D017152:Antibodies, Antiphospholipid; D007074:Immunoglobulin G
keywords: Antiphospholipid; TLR4; obstetric; trophoblast