title: In vitro effects of triiodothyronine on gene expression in mouse trophoblast cells.
abstract: The objective of the present study was to evaluate the effects of different doses of T3 (10(-4) M, 10(-7) M, 10(-9) M) on the in vitro gene expression of Tpbp, Prl3b1, VEGF, PGF, PL-1, and INFy in mouse trophoblast cells by real-time RT-PCR. Doses of 10(-7) and 10(-9) M T3 increased the mRNA levels of Tpbp, Pl3b1, VEGF, PGF, INFy and PL-1. In contrast, the dose of 10(-4) M reduced the gene expression of PL-1 and VEGF. T3 affected the gene expression of differentiation, hormonal, immune and angiogenic factors in mouse trophoblast cells.
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Silva
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
authors.forename: N M
authors.initials: NM
authors.lastname: Ocarino
authors.affiliation: Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil. Electronic address: serakidesufmg@gmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Serakides
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D015870:Gene Expression / Q000187:drug effects*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D051379:Mice; D000072483:Placenta Growth Factor; D010928:Placental Lactogen / Q000235:genetics / Q000378:metabolism; D011257:Pregnancy Proteins / Q000235:genetics / Q000378:metabolism; D033882:Transferrin-Binding Protein B / Q000235:genetics / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*; D014327:Trophoblasts / Q000187:drug effects* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C000608037:Pgf protein, mouse; D011257:Pregnancy Proteins; D033882:Transferrin-Binding Protein B; D042461:Vascular Endothelial Growth Factor A; D014284:Triiodothyronine; D000072483:Placenta Growth Factor; D007371:Interferon-gamma; D010928:Placental Lactogen
keywords: Culture; Differentiation; Mouse; Triiodothyronine; Trophoblast