title: MiR-143 and rat embryo implantation.
abstract: BACKGROUND
  To study the role of miR-143 during embryo implantation in rat.
  
  
  METHODS
  MiR-143 expression in rat early pregnancy was detected by Northern blot. The relation between miR-143 and Lifr predicted and confirmed by bioinformatics method, dual-luciferase activity assay, Western blot and immunohistochemistry. The role of miR-143 was detected by MTS, Edu and ranswell chamber assays.
  
  
  RESULTS
  The expression level of miR-143 on gestation day 5-8 (g.d. 5-8) was higher than on g.d. 3-4 in uteri of pregnant rat. MiR-143 was mainly localized in the superficial stroma/primary decidual zone, luminal and glandular epithelia. The expression of miR-143 was not significantly influenced by pseudopregnancy, but the activation of delayed implantation and experimentally induced decidualization significantly promoted miR-143 expression. Over-expression of miR-143 in human endometrial stromal cells (ESCs) inhibited cell proliferation, migration and invasion. Knockdown of miR-143 promoted cell proliferation and invasion. The results of recombinant luciferase reporters showed that miR-143 could bind to the 3¢-untranslated region (UTR) of leukemia inhibitory factor receptor (Lifr) to inhibit Lifr translation.
  
  
  CONCLUSIONS
  Uterine miR-143 may be involved in the successful pregnancy, especially during the process of blastocyst implantation through regulating Lifr.
  
  
  CONCLUSIONS
  This study may have the potential to provide new insights into the understanding of miR-143 function during embryo implantation.
authors.affiliation: Haidian Maternal & Child Health Hospital, Beijing 100080, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Tian
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing 100730, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Su
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qi
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing 100730, China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Jin
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Cadre Ward, China Mei-Tan General Hospital, Beijing 100028, China. Electronic address: chinawang2007@163.com.
authors.forename: Chun-Ling
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing 100730, China. Electronic address: genetic@263.net.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
authors.affiliation: Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Beijing 100730, China. Electronic address: hongfeixia@126.com.
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D010064:Embryo Implantation; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D053606:Receptors, OSM-LIF / Q000235:genetics; D014599:Uterus / Q000378:metabolism
chemical_list: C569508:MIRN143 microRNA, rat; D035683:MicroRNAs; D053606:Receptors, OSM-LIF; D004958:Estradiol
keywords: Embryo implantation; Lifr; Rat; Uterus; miR-143