title: [Molecular identification of 15 species of Ilex genus based on ITS sequence analysis ].
abstract: OBJECTIVE
  To establish molecular method for identification of Ilex plants using the internal transcribed spacer(ITS) bar- code.
  
  
  METHODS
  DNA was extracted using centrifugal columnar kits. The ITS sequences of all experimental samples were amplified and sequenced using a pair of universal primers. The alignment and the phylogenetic tree were performed in MEGA5. 0.
  
  
  RESULTS
  The length of ITS sequence in each species was 759 bp,in which there were 216 sites discrepancy.
  
  
  CONCLUSIONS
  DNA barcoding technology based on ITS sequence can be used to identify plants of Ilex genus.
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.forename: Feng-ming
authors.initials: FM
authors.lastname: Han
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
mesh_terms: D058893:DNA Barcoding, Taxonomic; D017931:DNA Primers; D018744:DNA, Plant / Q000235:genetics; D030017:Ilex / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018744:DNA, Plant