title: Conservation of proteo-lipid nuclear membrane fusion machinery during early embryogenesis.
abstract: The fusogenic lipid diacylglycerol is essential for remodeling gamete and zygote nuclear envelopes (NE) during early embryogenesis. It is unclear whether upstream signaling molecules are likewise conserved. Here we demonstrate PLCγ and its activator SFK1, which co-operate during male pronuclear envelope formation, also promote the subsequent male and female pronuclear fusion. PLCγ and SFK1 interact directly at the fusion site leading to PLCγ activation. This is accompanied by a spatially restricted reduction of PtdIns(4,5)P2. Consequently, pronuclear fusion is blocked by PLCγ or SFK1 inhibition. These findings identify new regulators of events in the early embryo and suggest a conserved "toolkit" of fusion machinery drives successive NE fusion events during embryogenesis.
authors.affiliation: a Cell Biophysics Laboratory; Cancer Research UK; London Research Institute; London, UK.
authors.forename: Richard D
authors.initials: RD
authors.lastname: Byrne
authors.forename: Selvaraju
authors.initials: S
authors.lastname: Veeriah
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Applebee
authors.forename: Banafshé
authors.initials: B
authors.lastname: Larijani
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D047108:Embryonic Development; D005260:Female; D006801:Humans; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D008561:Membrane Fusion / Q000235:genetics*; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D051966:Phospholipase C gamma / Q000235:genetics / Q000378:metabolism*; D012617:Sea Urchins / Q000196:embryology* / Q000235:genetics; D015053:Zygote / Q000378:metabolism
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D051966:Phospholipase C gamma
keywords: membrane fusion; nuclear envelope; phospholipase C; pronucleus; zygote formation