title: Glycosaminoglycan-targeted iron oxide nanoparticles for magnetic resonance imaging of liver carcinoma.
abstract: To develop an efficient probe for targeted magnetic resonance (MR) imaging of liver carcinoma, the surface modification of superparamagnetic iron oxide nanoparticles (SPIONs) was carried out by conjugating a naturally-occurring glycosaminoglycan with specific biological recognition to human hepatocellular liver carcinoma (HepG2) cells. These modified SPIOs have good water dispersibility, superparamagnetic property, cytocompatibility and high magnetic relaxivity for MR imaging. When incubated with HepG2 cells, they demonstrated significant cellular uptake and specific accumulation, as confirmed by Prussian blue staining and confocal microscopy. The in vitro MR imaging of HepG2 cells and in vivo MR imaging of HepG2 tumors confirmed their effectiveness for targeted MR imaging of liver carcinoma.
authors.affiliation: Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
authors.forename: Rui-Meng
authors.initials: RM
authors.lastname: Yang
authors.affiliation: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Chao-Ping
authors.initials: CP
authors.lastname: Fu
authors.affiliation: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Li
authors.affiliation: Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Xu
authors.affiliation: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
authors.forename: Ding-Yan
authors.initials: DY
authors.lastname: Yang
authors.affiliation: Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China.
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Fang
authors.affiliation: Department of Radiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, China. Electronic address: jiangxinqing888@163.com.
authors.forename: Xin-Qing
authors.initials: XQ
authors.lastname: Jiang
authors.affiliation: DSAPM Lab and PCFM Lab, Institute of Polymer Science, Department of Polymer and Materials Science, School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China; Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, School of Pharmaceutical Science, Sun Yat-sen University, Guangzhou 510006, China. Electronic address: ceszhlm@mail.sysu.edu.cn.
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D000588:Amines / Q000737:chemistry; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000737:chemistry / Q000378:metabolism; D006820:Hyaluronic Acid / Q000737:chemistry; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D018613:Microscopy, Confocal; D010316:Particle Size; D011859:Radiography; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000588:Amines; D003287:Contrast Media; D005290:Ferric Compounds; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D058185:Magnetite Nanoparticles; C000499:ferric oxide; D006820:Hyaluronic Acid
keywords: Bioactive glycosaminoglycan; Cellular uptake; Iron oxide nanoparticles; Liver carcinoma; Magnetic resonance imaging; Surface functionalization