title: Acute exposure to a precursor of advanced glycation end products induces a dual effect on the rat pancreatic islet function.
abstract: Aim. Chronic diseases are the leading cause of death worldwide. Advanced glycation end products, known as AGEs, are a major risk factor for diabetes onset and maintenance. Methylglyoxal (MG), a highly reactive metabolite of glucose, is a precursor for the generation of endogenous AGEs. Methods. In this current study we incubated in vitro pancreatic islets from adult rats in absence or presence of MG (10 μmol/l) with different concentrations of glucose and different metabolic components (acetylcholine, epinephrine, potassium, forskolin, and leucine). Results. Different effects of MG on insulin secretion were evidenced. In basal glucose stimulation (5.6 mM), MG induced a significant (P < 0.05) increase of insulin secretion. By contrast, in higher glucose concentrations (8.3 mM and 16.7 mM), MG significantly inhibited insulin secretion (P < 0.05). In the presence of potassium, forskolin, and epinephrine, MG enhanced insulin secretion (P < 0.05), while when it was incubated with acetylcholine and leucine, MG resulted in a decrease of insulin secretion (P < 0.05). Conclusion. We suggest that MG modulates the secretion activity of beta-cell depending on its level of stimulation by other metabolic factors. These results provide insights on a dual acute effect of MG on the pancreatic cells.
authors.affiliation: Institut Polytechnique LaSalle Beauvais, EGEAL-UP 2012.10.101., 19 rue Pierre Waguet, 60026 Beauvais Cedex, France.
authors.forename: Ghada
authors.initials: G
authors.lastname: Elmhiri
authors.affiliation: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Luiz Felipe
authors.identifier: 0000-0003-2211-3842
authors.initials: LF
authors.lastname: Barella
authors.affiliation: Environnement Périnatal et Croissance (EA4489), Equipe Dénutritions Maternelles Périnatales, SN4, Université de Lille 1, 59655 Villeneuve d'Ascq, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Vieau
authors.affiliation: INRA, UMR1198, Biologie du Développement et Reproduction, 78352 Jouy en Josas, France.
authors.forename: Sylvaine
authors.identifier: 0000-0002-6446-7246
authors.initials: S
authors.lastname: Camous
authors.affiliation: Laboratory of Secretion Cell Biology, Department of Biotechnology, Genetics and Cell Biology, State University of Maringá, 87020-900 Maringá, PR, Brazil.
authors.forename: Paulo C F
authors.initials: PC
authors.lastname: Mathias
authors.affiliation: Institut Polytechnique LaSalle Beauvais, EGEAL-UP 2012.10.101., 19 rue Pierre Waguet, 60026 Beauvais Cedex, France.
authors.forename: Latifa
authors.identifier: 0000-0002-8171-5707
authors.initials: L
authors.lastname: Abdennebi-Najar