title: Simultaneous separation and determination of phenolic acids, pentapeptides, and triterpenoid saponins in the root of Aster tataricus by high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry.
abstract: We established a qualitative method to analyze the main chemical compositions of the root of Aster tataricus. Most of the peaks were separated on a C(18) column packed with 5.0 μm particles, and 28 compounds were identified, including 11 chlorogenic acids, ten astins/asterinins, and seven astersaponins, four of which were reported for the first time from A. tataricus. Furthermore, we developed a reliable method for the simultaneous quantification of 3-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, astin A, astin B, astin C, astersaponin A, and astersaponin C, and the qualified separations were achieved only on a C18 column packed with 2.7 μm particles. The method was used to measure the concentrations of eight components in samples from two major producing areas in China, and the average contents in samples from Bozhou (Anhui) were higher than those in samples from Anguo (Hebei).
authors.affiliation: Research Department of Pharmacognosy, China Pharmaceutical University, Nanjing, China; Pharmaceutical College, Henan University, Kaifeng, China.
authors.forename: Dong-Xia
authors.initials: DX
authors.lastname: Zhao
authors.forename: Bing-Qiang
authors.initials: BQ
authors.lastname: Hu
authors.forename: Mian
authors.initials: M
authors.lastname: Zhang
authors.forename: Chao-Feng
authors.initials: CF
authors.lastname: Zhang
authors.forename: Xiang-Hong
authors.initials: XH
authors.lastname: Xu
mesh_terms: D031173:Aster Plant / Q000737:chemistry*; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000302:isolation & purification; D009842:Oligopeptides / Q000737:chemistry* / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D062385:Hydroxybenzoates; D009842:Oligopeptides; D012503:Saponins; D014315:Triterpenes; C017616:phenolic acid
keywords: Aster tataricus; Astersaponins; Astins; Chlorogenic acids; Qualitative and quantitative analysis