title: Identification of several hub-genes associated with periodontitis using integrated microarray analysis.
abstract: The aim of the present study was to identify differentially expressed genes and biological processes associated with periodontitis. In this study, the most significant 200 differentially expressed genes associated with periodontitis were identified using integrated analysis of multiple microarray data in combination with screening for genome‑wide relative significance and genome‑wide global significance. Gene Ontology (GO) enrichment analysis and pathway analysis were performed using the GO website and Kyoto Encyclopedia of Genes and Genomes (KEGG). A protein‑protein interaction (PPI) network was constructed based on the Search Tool for the Retrieval of Interacting Genes/Proteins. The top 200 differentially expressed genes were found to be highly associated with periodontitis. GO enrichment analyses revealed that the identified genes were significantly enriched in terms of response to organic substance, response to wounding and cell migration. The most common term of the KEGG pathway was cytokine‑cytokine receptor interaction. PPI network analysis indicated that interleukin (IL)8, IL1β, vascular endothelial growth factor A, intercellular adhesion molecule 1, PTGS2 and CXCL10 were hub genes, which formed numerous interactions with several genes. In conclusion, the present study identified numerous genes that were differentially expressed in periodontitis, as well as determined the biological pathways and PPI network associated with those genes.
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Xinxing
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Orthodontics, Jinan Stomatological Hospital, Jinan, Shandong 250001, P.R. China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthodontics, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Sterilization and Supply Center, School of Stomatology, Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
mesh_terms: D016022:Case-Control Studies; D019295:Computational Biology; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D055106:Genome-Wide Association Study; D006801:Humans; D058977:Molecular Sequence Annotation; D010518:Periodontitis / Q000235:genetics* / Q000378:metabolism; D060066:Protein Interaction Maps