title: Differentiation of iPSC to Mesenchymal Stem-Like Cells and Their Characterization.
abstract: Mesenchymal stem cells (MSC) are a unique population of adult stem cells that have the capacity to differentiate into numerous cell types as well as the ability to modulate the immune system. As such, MSC represent a promising stem cell population for use in the clinical treatment of a range of disorders involving tissue regeneration as well as the immune system. The lack of accessibility to MSC is currently limiting the use of MSC in mainstream clinical treatment strategies. It is therefore imperative for the future success of stem cell-based treatment approaches that are more reliable, and accessible sources of MSC are identified. The present chapter describes a method for generating MSC-like cells from induced pluripotent stem cells (iPSC), with equivalent growth and functional properties to parental MSC populations.
authors.affiliation: Colgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA, Australia. kim.hynes@adelaide.edu.au.|Mesenchymal Stem Cell Laboratory, School of Medical Sciences, University of Adelaide, Adelaide, SA, Australia. kim.hynes@adelaide.edu.au.|Mesenchymal Stem Cell Laboratory, Cancer Theme, Level 5 South, SAHMRI, North Terrace, Adelaide, SA, Australia. kim.hynes@adelaide.edu.au.
authors.forename: Kim
authors.initials: K
authors.lastname: Hynes
authors.affiliation: Colgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA, Australia.|Mesenchymal Stem Cell Laboratory, Cancer Theme, Level 5 South, SAHMRI, North Terrace, Adelaide, SA, Australia.|Mesenchymal Stem Cell Laboratory, School of Medical Sciences, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Menicanin
authors.affiliation: Mesenchymal Stem Cell Laboratory, Cancer Theme, Level 5 South, SAHMRI, North Terrace, Adelaide, SA, Australia.|Mesenchymal Stem Cell Laboratory, School of Medical Sciences, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Stan
authors.initials: S
authors.lastname: Gronthos
authors.affiliation: Colgate Australian Clinical Dental Research Centre, School of Dentistry, University of Adelaide, Adelaide, SA, Australia.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Bartold
mesh_terms: D050156:Adipogenesis / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D000067470:Cellular Reprogramming Techniques / Q000379:methods*; D020219:Chondrogenesis / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis / Q000187:drug effects
chemical_list: D003470:Culture Media
keywords: Characterization; Differentiation; Induced pluripotent stem cells; Mesenchymal stem cells; Serial passaging