title: Immunoglobulins G and lysozyme concentrations in canine fetal fluids at term of pregnancy.
abstract: In the dog, the endotheliochorial placenta allows only the 5% to 10% transfer of maternal antibodies to the fetus, but the timing and the factors influencing the immunoglobulin G (IgG) transplacental transport were not fully investigated. The aims of the present study were the following: (1) to assess the presence of both IgG and lysozyme in amniotic and allantoic fluids collected from fully developed and viable newborn puppies born by elective cesarean section at term and possible correlations between amniotic and allantoic IgG and lysozyme levels; (2) to verify possible differences in IgG and lysozyme concentrations between the two fluids; and (3) to detect possible differences in IgG and lysozyme fetal fluid levels in relation to the maternal breed body size and parity, as well as to the neonatal gender. The study, performed on 41 purebred bitches submitted to elective cesarean section at term, enrolled 142 puppies, 74 males and 68 females, born mature, viable, without gross malformations, and with a normal weight. At surgery, a total of 129 amniotic and 84 allantoic samples were collected for IgG and lysozyme analysis. Class G immunoglobulins and lysozyme were detected in both fluids, but IgG concentrations were higher (P < 0.01) in amniotic fluid. Moreover, a significant positive correlation (P < 0.01) between IgG amniotic and allantoic levels, but not for lysozyme, was observed. A significant effect of the maternal parity (P < 0.05), but not of the breed body size, on the amniotic IgG concentrations was found, whereas the newborn gender was not associated to different IgG or lysozyme amniotic or allantoic levels. Given the significant contributions of fetal fluids to fetal and neonatal health, the results reported that the amniotic and allantoic fluids play a role in the immune protection of the fetus/newborn also in canine species. However, additional research is needed to better elucidate both the origin of IgG and lysozyme and the factors influencing the wide interindividual variations.
authors.affiliation: Department of Veterinary Science and Public Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Dall'Ara
authors.affiliation: Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Milan, Italy. Electronic address: tea.meloni@unimi.it.
authors.forename: T
authors.initials: T
authors.lastname: Meloni
authors.affiliation: ECAR Resident, Ambulatorio Veterinario Pellegrini-Rota, Bergamo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rota
authors.affiliation: Department of Veterinary Science and Public Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Servida
authors.affiliation: Department of Veterinary Science and Public Health, Università degli Studi di Milano, Milan, Italy.
authors.forename: J
authors.initials: J
authors.lastname: Filipe
authors.affiliation: Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Milan, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Veronesi
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D004285:Dogs / Q000502:physiology*; D005260:Female; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy
chemical_list: D007074:Immunoglobulin G; D009113:Muramidase
keywords: Dog; Fetal fluids; Immunoglobulin G; Lysozyme