title: Heterologous expression and characterization of a novel halotolerant, thermostable, and alkali-stable GH6 endoglucanase from Thermobifida halotolerans.
abstract: A novel endoglucanase gene was cloned from Thermobifida halotolerans YIM 90462(T), designated as thcel6A for being a member of glycoside hydrolase family 6. The gene was 1332 bp long and encoded a 443-amino-acid protein with a molecular mass of 45.9 kDa. The purified recombinant endoglucanase had optimal activity at 55 °C and pH 8.5. Thcel6A showed high hydrolytic activities at 25-55 °C and retained 58% of initial activity after incubation at 90 °C for 1 h. It retained more than 80% of activity after incubation for 12 h at pH values from 4 to 12. Thcel6A displayed higher hydrolytic activities in 5-15% NaCl (w/v) than at 0% NaCl. Activity increased 2.5-fold after incubation with 20% (w/v) NaCl at 37 °C for 10 min. These properties suggest that this novel endoglucanase has potential for specific industrial application.
authors.affiliation: Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
authors.forename: Yi-Rui
authors.initials: YR
authors.lastname: Yin
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Hu
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.forename: Wael N
authors.initials: WN
authors.lastname: Hozzein
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Ming
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Nie
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics; D002480:Cellulase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D020536:Enzyme Activators / Q000378:metabolism; D004795:Enzyme Stability; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008970:Molecular Weight; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D020536:Enzyme Activators; D011994:Recombinant Proteins; D012965:Sodium Chloride; D002480:Cellulase