title: Bioactive compound synthesis of Ag nanoparticles from leaves of Melia azedarach and its control for mosquito larvae.
abstract: Larvicidal activity of synthesized Ag nanoparticles using 2,7.bis[2-[diethylamino]-ethoxy]fluorence isolate from the Melia azedarach leaves against Aedes aegypti and Culex quinquefasciatus. Six fractions were collected and concentrated, fraction three showed a single spot on TLC which was found to be a pure compound. The structures were elucidated by analyses of UV, MS, and NMR spectral data. The maximum mortality was fluorence against A. aegypti and C. quinquefasciatus (LC50 = 7.94, LC90 = 23.82 ppm and LC50 = 13.58 and LC90 = 40.03 ppm). The synthesized nanoparticles were characterized and confirmed as Ag nanoparticles by using UV-visible spectroscopy, XRD and HRTEM analysis. The maximum activity was observed in synthesized AgNPs against A. aegypti and C. quinquefasciatus (LC50 = 4.27 and 3.43 µg/mL; LC90 = 12.61 and 10.29 µg/mL). Rephrase test was studied to analyze the toxicological effects of Mesocyclops pehpeiensis for 24 h at synthesized AgNPs. This method is considered as an innovative alternative approach that can be used to control mosquitoes.
authors.affiliation: Unit of Aquatic Biodiversity, Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India. Electronic address: rramani8@hotmail.com.
authors.forename: R
authors.initials: R
authors.lastname: Ramanibai
authors.affiliation: Unit of Aquatic Biodiversity, Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
authors.forename: K
authors.initials: K
authors.lastname: Velayutham
mesh_terms: D000330:Aedes; D000818:Animals; D003465:Culex; D007306:Insecticides; D007814:Larva; D007928:Lethal Dose 50; D031586:Melia azedarach / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D009032:Mosquito Control / Q000379:methods*; D010936:Plant Extracts; D018515:Plant Leaves / Q000737:chemistry; D012834:Silver; D013056:Spectrophotometry, Ultraviolet; D014961:X-Ray Diffraction
chemical_list: D007306:Insecticides; D010936:Plant Extracts; D012834:Silver
keywords: Electron microscope study; Melia azedarach; Mosquito