title: Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7.
abstract: 8-Prenylnaringenin (8-PN) is a phytoestrogen with the highest estrogenic activity. The objective of the present study was to confirm the superiority of 8-PN on bone metabolisms and the estrogen receptor (ER) subtype mediating effects of 8-PN. The osteoblast MC3T3-E1 and osteoclast-like cell line RAW264.7 were treated with 17β-estradiol (10(-8) mol/L), genistein (10(-5) mol/L), daidzein (10(-5) mol/L), 8-PN (10(-5) mol/L) alone or in the presence of ERα antagonist MPP (10(-7) mol/L) and ERβ antagonist PTHPP (1.5 × 10(-7) mol/L). It has been found that 8-PN did not affect osteoblast proliferation, and that 8-PN increased alkaline phosphatase (ALP) activity, osteocalcin (OCN) concentrations, and the mineralized nodules. 8-PN inhibited RAW264.7 differentiating into osteoclasts and reduced the pit area of bone resorption. 8-PN could also inhibit the protein and mRNA expression of receptor activator of nuclear factor-κB ligand (RANKL) in osteoblasts, and conversely promote the expression of osteoprotegerin (OPG). These effects of 8-PN were mainly inhibited not by PTHPP but by MPP and they were weaker than estrogen's effects but stronger than those of genistein and daidzein. In conclusion, the effects of 8-PN on promoting osteoblastic bone formation and inhibiting osteoclastic bone resorption were mediated by ERα instead of ERβ and the efficacy was more potent than that of the two classic phytoestrogens: genistein and daidzein.
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Lumei
authors.initials: L
authors.lastname: Kang
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Qiren
authors.initials: Q
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: He
authors.affiliation: State Key Laboratory of Food Science and Technology, Medical School, Nanchang University Bayi Road 461, Nanchang, Jiangxi Province, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Peng
keywords: 8-Prenylnaringenin; daidzein; estrogen receptor; genistein; osteoblast; osteoclast