title: Susceptibility of Stenotrophomonas maltophilia clinical isolates to antibiotics and contact lens multipurpose disinfecting solutions.
abstract: OBJECTIVE
  To determine the susceptibility of Stenotrophomonas maltophilia to various antibiotics and contact lens multipurpose disinfecting solutions.
  
  
  METHODS
  Forty S. maltophilia strains from contact lens cases, contact lenses, or eye swabs of contact lens wearers including 27 asymptomatic wearers and 13 keratitis patients were examined for their susceptibility to different antibiotics, using a disc diffusion assay, and to multipurpose disinfecting solutions using a broth microdilution method.
  
  
  RESULTS
  Certain strains were resistant to aztreonum (15%), imipenem (93%), chroramphenicol (13%), and cefepime (8%). Two of those strains were multidrug resistant. All strains were sensitive to trimethoprim-sulfamethoxazole, tigecycline, ceftazidime, and fluoroquinolones. Overall, the minimum inhibitory concentration (MIC) for all strains was significantly higher (P < 0.05) for AQuify (50% dilution) and OPTI-FREE RepleniSH (25%) than all other multipurpose contact lens disinfecting solutions (MPDS) (3%-14%, except RepleniSH versus MeniCare Soft [14%]). AQuify, OPTI-FREE RepleniSH, and MeniCare Soft had significantly higher minimum bactericidal concentrations (undiluted MPDS) than other disinfecting solutions (P < 0.05).
  
  
  CONCLUSIONS
  The Australian ocular isolates of S. maltophilia remain susceptible to trimethoprim-sulfamethozole, tigecycline, and most fluoroquinolones. However, the isolates showed resistance to certain multipurpose disinfecting solutions.
authors.affiliation: Department of Ophthalmology, Kinki University Faculty of Medicine, Osaka, Japan Brien Holden Vision Institute, University of New South Wales, Sydney, Australia.
authors.forename: Keizo
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Brien Holden Vision Institute, University of New South Wales, Sydney, Australia School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: School of Optometry and Vision Science, University of New South Wales, Sydney, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: Willcox
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents; D001315:Australia; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003261:Contact Lenses / Q000382:microbiology; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods* / Q000592:standards; D004352:Drug Resistance, Microbial; D004866:Equipment Contamination / Q000517:prevention & control; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy; D006801:Humans; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D020615:Stenotrophomonas maltophilia / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D018639:Contact Lens Solutions; D004202:Disinfectants
keywords: Stenotrophomonas; antibiotics; disinfection