title: Magnetic nanoparticle clusters for photothermal therapy with near-infrared irradiation.
abstract: In this study, the photothermal effect of magnetic nanoparticle clusters was firstly reported for the photothermal ablation of tumors both in vitro in cellular systems but also in vivo study. Compared with individual magnetic Fe3O4 nanoparticles (NPs), clustered Fe3O4 NPs can result in a significant increase in the near-infrared (NIR) absorption. Upon NIR irradiation at 808 nm, clustered Fe3O4 NPs inducing higher temperature were more cytotoxic against A549 cells than individual Fe3O4 NPs. We then performed in vivo photothermal therapy (PTT) studies and observed a promising tumor treatment. Compared with PBS and individual magnetic Fe3O4 NPs by NIR irradiation, the clustered Fe3O4 NPs treatment showed a higher therapeutic efficacy. The treatment effects of clustered Fe3O4 NPs with different time of NIR illumination were also evaluated. The result indicated that a sustained high temperature generated by NIR laser with long irradiation time was more effective in killing tumor cells. Furthermore, histological analysis of H&E staining and TUNEL immunohistological assay were further employed for antitumor efficacy assessment of PTT against A549 tumors.
authors.affiliation: School of Pharmacy & Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai 201203, China.
authors.forename: Shun
authors.initials: S
authors.lastname: Shen
authors.affiliation: School of Pharmacy & Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai 201203, China; Pancreatic Disease Institute, Department of Pancreatic Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers & Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Department of Integrative Oncology, Shanghai Cancer Center, Department of Oncology, Shanghai Medical University, Fudan University, Shanghai 200032, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.affiliation: School of Pharmacy & Key Laboratory of Smart Drug Delivery, Fudan University, Shanghai 201203, China.
authors.forename: Xinguo
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Pancreatic Disease Institute, Department of Pancreatic Surgery, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers & Department of Macromolecular Science, Fudan University, Shanghai 200433, China. Electronic address: wlyang@fudan.edu.cn.
authors.forename: Wuli
authors.initials: W
authors.lastname: Yang
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D007259:Infrared Rays; D058185:Magnetite Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D010789:Phototherapy / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles
keywords: Fe(3)O(4); Magnetic nanoparticle clusters; Photothermal therapy; Tumor