title: Effects of recombinant human bone morphogenetic protein 7 (rhBMP-7) on the behaviour of oral squamous cell carcinoma: a preliminary in vitro study.
abstract: We investigated the effects of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the behaviour of oral keratinocytes and head and neck squamous cell carcinoma (SCC) cells in vitro. Expression of all three BMP receptors was high (p<0.01), and rhBMP-7 exhibited significant dose-related inhibitory effects on the doubling time and viability of cancer cells (p<0.01), but not on the proliferation or viability of oral keratinocytes. It elicited no significant effect on the invasion of Matrigel in SCC of the head and neck. Results indicate that in cell culture, rhBMP-7 exerts antineoplastic effects. This should be tested in an orthotopic animal model to more closely replicate in vivo effects.
authors.affiliation: Infection and immunity Research Group, Glasgow Dental Hospital & School, Glasgow, UK.
authors.forename: D F
authors.initials: DF
authors.lastname: Lappin
authors.affiliation: Department of Oral & Maxillofacial Surgery, Manchester Royal Infirmary, Central Manchester University Hospitals NHS Foundation Trust, Manchester M13 9WL, UK. Electronic address: m.abuserriah@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Abu-Serriah
authors.affiliation: Unit of Oral and Maxillofacial Pathology, University of Sheffield, Sheffield, UK.
authors.forename: K D
authors.initials: KD
authors.lastname: Hunter
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000032:analysis; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects; D016895:Culture Media, Serum-Free; D006801:Humans; D007202:Indicators and Reagents; D015603:Keratinocytes / Q000187:drug effects*; D009062:Mouth Neoplasms / Q000473:pathology*; D009361:Neoplasm Invasiveness; D009596:Nitrophenols; D009943:Organophosphorus Compounds
chemical_list: D000970:Antineoplastic Agents; C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D016895:Culture Media, Serum-Free; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; C498211:BMPR1A protein, human; C498214:BMPR1B protein, human; C498218:BMPR2 protein, human; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: In vitro; Oral cancer; Squamous cell carcinoma; rhBMP-7; rhOP-1