title: Quercetin promotes cell apoptosis and inhibits the expression of MMP-9 and fibronectin via the AKT and ERK signalling pathways in human glioma cells.
abstract: Gliomas are the most common and malignant primary brain tumours and are associated with a poor prognosis despite the availability of multiple therapeutic options. Quercetin, a traditional Chinese medicinal herb, is an important flavonoid and has anti-cancer activity. Here, we evaluated whether quercetin could inhibit glioma cell viability and migration and promote apoptosis. The treatment of U87-MG glioblastoma and U251 and SHG44 glioma cell lines with different concentrations of quercetin inhibited cell viability in a dose-dependent manner. Wound healing assays indicated that quercetin significantly decreased glioma cell migration. β-galactosidase staining, DNA staining and Annexin V-EGF/PI double staining assays demonstrated that quercetin promoted cell senescence and apoptosis. In addition, the protein levels of p-AKT, p-ERK, Bcl-2, matrix metallopeptidase 9 (MMP-9) and fibronectin (FN) were significantly reduced following quercetin treatment. Therefore, we conclude that quercetin might inhibit the viability and migration and promote the senescence and apoptosis of glioma cells by suppressing the Ras/MAPK/ERK and PI3K/AKT signalling pathways. Quercetin might be a potential candidate for the clinical treatment of glioma.
authors.affiliation: Guangdong Provincial Key Laboratory for Breast Cancer Diagnosis and Treatment, Cancer Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China; Center for Neuroscience, Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Hong-Chao
authors.initials: HC
authors.lastname: Pan
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Mei
authors.affiliation: Guangdong Provincial Key Laboratory for Breast Cancer Diagnosis and Treatment, Cancer Hospital of Shantou University Medical College, Shantou 515041, Guangdong, China. Electronic address: ycui27@gmail.com.
authors.forename: Yu-Kun
authors.initials: YK
authors.lastname: Cui
authors.affiliation: Center for Neuroscience, Shantou University Medical College, Shantou 515041, Guangdong, China. Electronic address: neuromancn@aliyun.com.
authors.forename: Wei-Jiang
authors.initials: WJ
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*
chemical_list: D005353:Fibronectins; D061965:Matrix Metalloproteinase Inhibitors; D011794:Quercetin; D051057:Proto-Oncogene Proteins c-akt; D020780:Matrix Metalloproteinase 9
keywords: Apoptosis; Glioma; Matrix metallopeptidase 9 (MMP-9); Quercetin; p-AKT; p-ERK