title: Transgenerational effects of binge drinking in a primate model: implications for human health.
abstract: OBJECTIVE
  To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development.
  
  
  METHODS
  Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating.
  
  
  METHODS
  National primate research center.
  
  
  METHODS
  Adult female rhesus monkeys.
  
  
  METHODS
  Binge levels of ethanol, given twice weekly for 6 months before a standard in vitro fertilization cycle with or without embryo culture. With in vivo development, ethanol treatment continued until pregnancy was identified.
  
  
  METHODS
  Oocyte and cumulus/granulosa cell gene expression, embryo development to blastocyst, and pregnancy rate.
  
  
  RESULTS
  Embryo development in vitro was reduced; changes were found in oocyte and cumulus cell gene expression; and spontaneous abortion during very early gestation increased.
  
  
  CONCLUSIONS
  This study provides evidence that binge drinking can affect the developmental potential of oocytes even after alcohol consumption has ceased.
authors.affiliation: California National Primate Research Center, University of California, Davis, California; Department of Obstetrics and Gynecology, University of California, Davis, California. Electronic address: cavandevoort@ucdavis.edu.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: VandeVoort
authors.affiliation: California National Primate Research Center, University of California, Davis, California.
authors.forename: Kristin N
authors.initials: KN
authors.lastname: Grimsrud
authors.affiliation: The Fels Institute for Cancer Research & Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania; Department of Animal Science, Michigan State University, East Lansing, Michigan.
authors.forename: Uros
authors.initials: U
authors.lastname: Midic
authors.affiliation: The Fels Institute for Cancer Research & Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Namdori
authors.initials: N
authors.lastname: Mtango
authors.affiliation: The Fels Institute for Cancer Research & Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania; Department of Animal Science, Michigan State University, East Lansing, Michigan; Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania.
authors.forename: Keith E
authors.initials: KE
authors.lastname: Latham
mesh_terms: D000818:Animals; D063425:Binge Drinking / Q000150:complications* / Q000473:pathology*; D015988:Cohort Effect; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D006304:Health Status; D006801:Humans; D008253:Macaca mulatta; D023421:Models, Animal; D009865:Oocytes / Q000187:drug effects / Q000473:pathology; D011247:Pregnancy; D018873:Pregnancy Rate / Q000639:trends
chemical_list: D000431:Ethanol
keywords: Cumulus cells; cDNA array; fetal alcohol syndrome; granulosa cells; reproduction; transcriptome