title: Streptococcus tangierensis sp. nov. and Streptococcus cameli sp. nov., two novel Streptococcus species isolated from raw camel milk in Morocco.
abstract: Biochemical and molecular genetic studies were performed on two unidentified Gram-stain positive, catalase and oxidase negative, non-hemolytic Streptococcus-like organisms recovered from raw camel milk in Morocco. Phenotypic characterization and comparative 16S rRNA gene sequencing demonstrated that the two strains were highly different from each other and that they did not correspond to any recognized species of the genus Streptococcus. Phylogenetic analysis based on 16S rRNA gene sequences showed the unidentified organisms each formed a hitherto unknown sub-line within the genus Streptococcus, displaying a close affinity with Streptococcus moroccensis, Streptococcus minor and Streptococcus ovis. DNA G+C content determination, MALDI-TOF mass spectrometry and biochemical tests demonstrated the bacterial isolates represent two novel species. Based on the phenotypic distinctiveness of the new bacteria and molecular genetic evidence, it is proposed to classify the two strains as Streptococcus tangierensis sp. nov., with CCMM B832(T) (=LMG 27683(T)) as the type strain, and Streptococcus cameli sp. nov., with CCMM B834(T) (=LMG 27685(T)) as the type strain.
authors.affiliation: Laboratoire de Microbiologie et Biologie Moléculaire (LMBM), Centre National pour la Recherche Scientifique et Technique (CNRST), Angle av. Allal El Fassi, av.des FAR, Quartier Hay Ryad, Nations Unies, B.P. 8027, 10102, Rabat, Morocco.
authors.forename: Zaina
authors.initials: Z
authors.lastname: Kadri
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.forename: Mouna
authors.initials: M
authors.lastname: Ouadghiri
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.forename: Maarten
authors.initials: M
authors.lastname: Aerts
authors.forename: El Mostafa
authors.initials: el M
authors.lastname: Elfahime
authors.forename: Omar El
authors.initials: OE
authors.lastname: Farricha
authors.forename: Jean
authors.initials: J
authors.lastname: Swings
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amar
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002162:Camelus; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008892:Milk / Q000382:microbiology*; D008969:Molecular Sequence Data; D009018:Morocco; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013291:Streptococcus / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020543:Proteome; D012336:RNA, Ribosomal, 16S