title: Study on matrix metalloproteinase 1 and 2 gene expression and NO in dairy cows with ovarian cysts.
abstract: The objective of this study was to investigate how changes in total nitric oxide (NO) and ovarian matrix metalloproteinase (MMP)-1 and MMP-2 correlated with luteinizing hormone (LH) and estradiol (E2) in infertile dairy cows with ovarian cysts. Holstein cows (n=21 infertile cows and 19 fertile) were studied during their estrous phase to minimize hormone fluctuations. Blood LH, E2 and NO were measured. Expression of the MMP-1 and MMP-2 genes in the ovaries was measured by real-time PCR and immunohistochemistry. LH, E2 and NO were less in infertile cows with ovarian cysts than in fertile cows (P<0.05). The mRNAs of MMP-1 and MMP-2 were less in infertile cows with ovarian cysts than in fertile cows (P<0.05). The immunohistochemical results showed that MMP-1 and MMP-2 genes were expressed in different parts of the ovarian tissues, including granulosa and theca cells of preovulatory follicles, epithelial follicular cells of small follicles, stromal cells and endothelial cells of blood vessels. The results showed that a decrease in MMP-1 and MMP-2 gene expression is accompanied with a decrease in NO concentrations in infertile cows affected with ovarian cysts. The expression of these marker genes might be risk factors of infertility in cows and might correlate with the hormonal profile. The present study suggests that the abnormal expression of the MMP-1/2 gene might be an important marker of ovarian follicular cysts in dairy cows.
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Ali M
authors.initials: AM
authors.lastname: Mutlag
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China. Electronic address: zhiqyang2006@163.com.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Jiaren
authors.initials: J
authors.lastname: Meng
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Xurong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China.
authors.forename: Guibo
authors.initials: G
authors.lastname: Wang
authors.affiliation: Engineering & Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture, Key Laboratory of Animal Drug Project of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, 335 Jiangouyan St., Qilihe Dist., Lanzhou, Gansu 730050, China. Electronic address: lzjianxil@163.com.
authors.forename: Jianxi
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000378:metabolism*; D004958:Estradiol / Q000097:blood / Q000378:metabolism; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism*; D010048:Ovarian Cysts / Q000378:metabolism / Q000662:veterinary*; D014018:Tissue Distribution
chemical_list: D015415:Biomarkers; D009569:Nitric Oxide; D004958:Estradiol; D007986:Luteinizing Hormone; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1
keywords: Estradiol; Infertility; Luteinizing hormone; Matrix metalloproteinase; Nitric oxide; Ovarian follicular cyst