title: Crystallization and preliminary crystallographic studies of the hypothetical protein BPSL1038 from Burkholderia pseudomallei.
abstract: Melioidosis is an infectious disease caused by the pathogenic bacterium Burkholderia pseudomallei. Whole-genome sequencing revealed that the B. pseudomallei genome includes 5855 coding DNA sequences (CDSs), of which ∼25% encode hypothetical proteins. A pathogen-associated hypothetical protein, BPSL1038, was overexpressed in Escherichia coli, purified and crystallized using vapour-diffusion methods. A BPSL1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 Å resolution. It belonged to space group C2221, with unit-cell parameters a = 85.36, b = 115.63, c = 46.73 Å. The calculated Matthews coefficient (VM) suggests that there are two molecules per asymmetric unit, with a solvent content of 48.8%.
authors.affiliation: Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
authors.forename: Sofiyah
authors.initials: S
authors.lastname: Shaibullah
authors.affiliation: Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
authors.forename: Nurhikmah
authors.initials: N
authors.lastname: Mohd-Sharif
authors.affiliation: Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor 43400, Malaysia.
authors.forename: Kok Lian
authors.initials: KL
authors.lastname: Ho
authors.affiliation: Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
authors.forename: Mohd
authors.initials: M
authors.lastname: Firdaus-Raih
authors.affiliation: School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
authors.forename: Sheila
authors.initials: S
authors.lastname: Nathan
authors.affiliation: INTI International University, Bandar Baru Nilai, Nilai, Negeri Sembilan 71800, Malaysia.
authors.forename: Rahmah
authors.initials: R
authors.lastname: Mohamed
authors.affiliation: Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia.
authors.forename: Chyan Leong
authors.initials: CL
authors.lastname: Ng
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D016957:Burkholderia pseudomallei / Q000737:chemistry*; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray
chemical_list: D001426:Bacterial Proteins
keywords: BPSL1038; Burkholderia pseudomallei; hypothetical protein