title: A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources.
abstract: Glucose and glycerol are useful carbon sources for the cultivation of Aurantiochytrium limacinum SR21. Glucose facilitates rapid growth and lipid synthesis, and glycerol promotes the accumulation of docosahexaenoic acid (DHA) in A. limacinum SR21. To improve the DHA productivity of A. limacinum SR21, shake flask and fed-batch cultures were performed using glucose and glycerol as mixed carbon sources (MCSs). Along with optimization of the MCSs, the best DHA yield and productivity (32.36 g/L and 337.1 mg/L/h) were obtained via fed-batch fermentation with maintenance of a constant air supply. The DHA productivity was 15.24% higher than that obtained using glucose as single carbon source (SCS). This study presents a highly efficient and economic strategy for the production of DHA by A. limacinum SR21.
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Ruijie
authors.initials: R
authors.lastname: Liu
authors.affiliation: Wilmar Biotechnology Research & Development Center Co., Ltd., Shanghai, China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address: mingchang@aliyun.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yuanfa
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Qingzhe
authors.initials: Q
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Xingguo
authors.initials: X
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis / Q000187:drug effects; D061249:Batch Cell Culture Techniques; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D058009:Stramenopiles / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004281:Docosahexaenoic Acids; D002244:Carbon; D005947:Glucose; D005990:Glycerol
keywords: Aurantiochytrium limacinum SR21; DHA; Glucose; Glycerol; Mixed carbon sources