title: Excretory-secretory antigens: a suitable candidate for immunization against ocular toxoplasmosis in a murine model.
abstract: Toxoplasmosis, responsible for ocular impairment, is caused by Toxoplasma gondii. We investigated the effect of Toxoplasma excretory-secretory antigens (ESA) on parasite load and distribution in the eye tissue of a murine model. Case and control groups were immunized with ESA and PBS, respectively. Two weeks after the second immunization, the mice were challenged intraperitoneally with virulent RH strain of Toxoplasma; eye tissue samples of both groups were collected daily (days 1, 2, 3, and the last day before death). Parasite load was determined using real-time quantitative PCR targeted at the B1 gene. Compared to the control group, infected mice that received ESA vaccine presented a considerable decrease in parasite load in the eye tissue, demonstrating the effect of ESA on parasite load and distribution. Diminution of parasite load in mouse eye tissue indicated that ESA might help control disease-related complications and could be a valuable immunization candidate against ocular toxoplasmosis.
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Ophthalmology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Kiumars
authors.initials: K
authors.lastname: Norouzpour Deilami
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: daryanii@yahoo.com.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Daryani
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran. Electronic address: ehsanahmadpour@gmail.com.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Ahmadpour
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sharif
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Yousef
authors.initials: Y
authors.lastname: Dadimoghaddam
authors.affiliation: Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran; Department of Parasitology and Mycology, Sari Medical School, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Shahabeddin
authors.initials: S
authors.lastname: Sarvi
authors.affiliation: Biostatistics Department, Mazandaran University of Medical Sciences, Sari, Iran.
authors.forename: Ahad
authors.initials: A
authors.lastname: Alizadeh
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000008:administration & dosage* / Q000276:immunology / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D059208:Parasite Load; D016052:Protozoan Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014122:Toxoplasma / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014126:Toxoplasmosis, Ocular / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; C408624:excretory-secretory antigen, Toxoplasma
keywords: Excretory–secretory antigens; Ocular toxoplasmosis; Parasite load; Q-PCR; Vaccine