title: IL-17 Enhances Chemotaxis of Primary Human B Cells during Asthma.
abstract: IL-17 is a pro-inflammatory mediator that is believed to play a critical role in regulating tissue inflammation during asthma, COPD, as well as other inflammatory disorders. The level of expression of IL-17 has been shown to be upregulated in lung bronchial tissue of asthmatic patients. Several reports have provided further evidence that this cytokine could play a key role in enhancing the migration of inflammatory as well as structural cells of the bronchial lung tissue during asthma and COPD. B cell infiltration to sites of inflammation during inflammatory disorders such as bowel disease, asthma and COPD has been reported. Accordingly, in this study we hypothesized that IL-17 may exert a chemotactic effect on primary B cells during asthma. We observed that B cells from asthmatic patients expressed significantly higher levels of IL-17RA and IL-17RC, compared to those of healthy subjects. Using an in-vitro migration assay, B cells were shown to migrate towards both IL-17A and IL-17F. Interestingly, blocking IL-17A and IL-17F signaling using either anti-IL-17R antibodies or MAP kinase inhibitors prevented in vitro migration of B cell towards IL-17. These observations indicate a direct chemotactic effect of IL-17 cytokines on primary peripheral blood B cells with higher effect being on asthmatic B cells. These findings revealed a key role for IL-17 in enhancing the migration of B cells to the lung tissue during asthma or COPD.
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Rabih
authors.initials: R
authors.lastname: Halwani
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Roua
authors.initials: R
authors.lastname: Al-Kufaidy
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Vazquez-Tello
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mary Angeline
authors.initials: MA
authors.lastname: Pureza
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia; Pulmonary Medicine Department, University Sleep Disorders Center, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Ahmed S
authors.initials: AS
authors.lastname: BaHammam
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia; Department of Medicine, Pulmonary Division-ICU, King Saud University for health sciences, Riyadh, Saudi Arabia.
authors.forename: Hamdan
authors.initials: H
authors.lastname: Al-Jahdali
authors.affiliation: Division of Thoracic Surgery, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Sami A
authors.initials: SA
authors.lastname: Alnassar
authors.affiliation: Meakins-Christie Laboratories, McGill University, Montreal, Quebec, Canada.
authors.forename: Qutayba
authors.initials: Q
authors.lastname: Hamid
authors.affiliation: Prince Naif Center for Immunology Research, department of pediatrics, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Saleh
authors.initials: S
authors.lastname: Al-Muhsen
mesh_terms: D000328:Adult; D001249:Asthma / Q000276:immunology*; D001402:B-Lymphocytes / Q000378:metabolism*; D001980:Bronchi / Q000276:immunology; D054382:Chemokine CXCL13 / Q000276:immunology; D002633:Chemotaxis / Q000187:drug effects*; D004789:Enzyme Activation; D005260:Female; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D007249:Inflammation / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology; D008297:Male; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology*; D011725:Pyridines / Q000494:pharmacology; D018123:Receptors, Interleukin / Q000276:immunology; D053722:Receptors, Interleukin-17 / Q000276:immunology / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C110663:CXCL13 protein, human; C508606:CXCL8 protein, human; D054382:Chemokine CXCL13; C508717:IL17A protein, human; C440021:IL17F protein, human; C109985:IL17RA protein, human; C448419:IL17RC protein, human; D007093:Imidazoles; D020381:Interleukin-17; D016209:Interleukin-8; D011725:Pyridines; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580