title: Development of a sensor to study the DNA conformation using molecular logic gates.
abstract: This communication reports our investigations on the Fluorescence Resonance Energy Transfer (FRET) between two laser dyes Acriflavine and Rhodamine B in absence and presence of DNA at different pH. It has been observed that energy transfer efficiency is largely affected by the presence of DNA as well as the pH of the system. It is well known that with increase in pH, DNA conformation changes from double stranded to single stranded (denaturation) and finally form random coil. Based on our experimental results two different types of molecular logic gates namely, XOR and OR logic have been demonstrated which can be used to have an idea about DNA conformation in solution.
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India.
authors.forename: Arpan Datta
authors.initials: AD
authors.lastname: Roy
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India.
authors.forename: Dibyendu
authors.initials: D
authors.lastname: Dey
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India.
authors.forename: Jaba
authors.initials: J
authors.lastname: Saha
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India.
authors.forename: Santanu
authors.initials: S
authors.lastname: Chakraborty
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India.
authors.forename: D
authors.initials: D
authors.lastname: Bhattacharjee
authors.affiliation: Department of Physics, Tripura University, Suryamaninagar 799022, Tripura, India. Electronic address: sa_h153@hotmail.com.
authors.forename: Syed Arshad
authors.initials: SA
authors.lastname: Hussain
mesh_terms: D000167:Acriflavine / Q000737:chemistry* / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D007364:Intercalating Agents / Q000737:chemistry / Q000494:pharmacology; D008128:Logic; D009690:Nucleic Acid Conformation / Q000187:drug effects; D012235:Rhodamines / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D007364:Intercalating Agents; D012235:Rhodamines; D000167:Acriflavine; D004247:DNA; C029773:rhodamine B
keywords: Coil formation; Denaturation; Deoxyribonucleic acid (DNA); FRET; Molecular logic gate; Sensor