title: [Protective effect of serum containing n-butanol fraction from Gynostemma pentaphyllum on NG108-15 cell apoptosis induced by Aβ25.35 protein].
abstract: OBJECTIVE
  To investigate the inhibition effect of serum containing n-butanol fractions from Gynostemma pentaphyllum on NG108-15 cell apoptosis induced by Aβ25-35 protein in vitro.
  
  
  METHODS
  The apoptosis of NG108-15 cells induced by Aβ25-35 protein in vitro was evaluated by immunofluorescence and flow cytometry assay. The cellular Caspase-3 level during the apoptosis was determined by ELISA.
  
  
  RESULTS
  The serum containing n-butanol fractions from Gynostemma pentaphyllum significantly inhibited the NG108-15 cells apoptosis induced by Aβ25-35 protein in vitro,and decreased the cellular Caspase-3 level.
  
  
  CONCLUSIONS
  The inhibition effect of n-butanol fractions from Gynostemma pentaphyllum on NG108-15 cell apoptosis induced by Aβ25.35 protein is likely related to its potency on reducing of cellular Caspase-3 level.
authors.forename: Yan-chun
authors.initials: YC
authors.lastname: Wu
authors.forename: Zhen-guo
authors.initials: ZG
authors.lastname: Zhong
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Liu
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Wang
authors.forename: Wen-yan
authors.initials: WY
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Cui
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3; D002460:Cell Line; D029774:Gynostemma / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D044967:Serum
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D010936:Plant Extracts; C074636:amyloid beta-protein (25-35); D020001:1-Butanol; D053148:Caspase 3