title: Blue green alga mediated synthesis of gold nanoparticles and its antibacterial efficacy against Gram positive organisms.
abstract: Biofunctionalized gold nanoparticles (AuNPs) play an important role in design and development of nanomedicine. Synthesis of AuNPs from biogenic materials is environmentally benign and possesses high bacterial inhibition and bactericidal properties. In the present study, blue green alga Spirulina platensis protein mediated synthesis of AuNPs and its antibacterial activity against Gram positive bacteria is discussed. AuNPs were characterized using Ultraviolet-visible (UV-vis) spectroscopy, Fluorescence spectroscopy, Fourier Transform-Infrared (FTIR) spectroscopy, Raman spectroscopy, High Resolution-Transmission Electron Microscopy (HR-TEM) and Energy Dispersive X-ray analysis (EDAX). Stable, well defined AuNPs of smaller and uniform shape with an average size of ~ 5 nm were obtained. The antibacterial efficacy of protein functionalized AuNPs were tested against Gram positive organisms Bacillus subtilis and Staphylococcus aureus.
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: K S Uma
authors.initials: KS
authors.lastname: Suganya
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India. Electronic address: govindtu@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Govindaraju
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: V Ganesh
authors.initials: VG
authors.lastname: Kumar
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: T Stalin
authors.initials: TS
authors.lastname: Dhas
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: V
authors.initials: V
authors.lastname: Karthick
authors.affiliation: Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore 632115, India.
authors.forename: G
authors.initials: G
authors.lastname: Singaravelu
authors.affiliation: Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600113, India.
authors.forename: M
authors.initials: M
authors.lastname: Elanchezhiyan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000458:Cyanobacteria / Q000737:chemistry*; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D006046:Gold
keywords: Antibacterial activity; Biofunctionalization; Gold nanoparticles; Gram positive organisms; Spirulina platensis