title: Runx1t1 regulates the neuronal differentiation of radial glial cells from the rat hippocampus.
abstract: The brain has the highest Runx1t1 level relative to the levels in other organs. Runx1t1 might have a regulatory function as a transcriptional corepressor in the differentiation/development of the nervous system. Neurogenesis requires factors that regulate the proliferation of progenitors and activate the neuronal differentiation process. However, the precise role of Runx1t1 in hippocampal neurogenesis is unclear. We knocked down Runx1t1 in hippocampal radial glial cells (RGCs) with Runx1t1-RNA interference using lentiviral vectors. We also used LV-Runx1t1 to induce Runx1t1 overexpression in vitro. We have provided experimental evidence that decreased Runx1t1 expression reduced the neuronal differentiation of RGCs, and increased Runx1t1 expression caused a greater number of RGCs to differentiate into neurons. We have concluded that Runx1t1 could be involved in the process through which RGCs differentiate into neurons.
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China.
authors.forename: Zou
authors.initials: Z
authors.lastname: Linqing
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China jguohua@ntu.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Guohua
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Haoming
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Xuelei
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Jianbing
authors.affiliation: Department of Human Anatomy, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu, People's Republic of China; Department of Human Anatomy and Histoembryology, Medical College of Soochow University, Suzhou, People's Republic of China.
authors.forename: Tian
authors.initials: T
authors.lastname: Meiling
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000502:physiology*; D063928:Ependymoglial Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000166:cytology; D058953:Neural Stem Cells / Q000166:cytology*; D055495:Neurogenesis / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D000075142:RUNX1 Translocation Partner 1 Protein; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000378:metabolism*; D014162:Transfection
chemical_list: D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; C000603993:RUNX1T1 protein, rat; D014157:Transcription Factors
keywords: Differentiation; Hippocampus; Neurogenesis; Radial glial cells; Runx1t1