title: Metabolomics of the aqueous humor in the rat glaucoma model induced by a series of intracamerular sodium hyaluronate injection.
abstract: Glaucoma models are helpful to study disease characteristics and to design new therapeutic options. Metabolomic profiling approach have been used to elucidating the molecular characteristics of the aqueous humor. Juvenile male Wistar rats experimental (n = 15) and controls (n = 6) were used for these studies. Experimental rats received weekly intracamerular injection of 25 µl of sodium hyaluronate in the left eye and sterile saline solution in the right eye, consecutively for ten weeks. Rats were subjected to anterior/posterior eye segment examinations, intraocular pressure (IOP), and flash electroretinograms (ERG). The aqueous humor was collected at endpoints and analyzed by Nuclear Magnetic Resonance. Elevated IOP and significant reduction of a, b waves and amplitude of oscillatory potential was observed in the left eyes compared to control eyes. The aqueous humor metabolomic profile from control and the experimental eyes were compared. Concentrations of metabolites (amino acids, lipids and carbohydrates) significantly changed after the sodium hyaluronate injections series, compared to the sham-operated eyes. Metabolic changes in the hypertensive eyes correlated with the impaired retinal function. Observed metabolomic changes in aqueous humor in hypertensive state may play a significant role in glaucoma pathogenesis.
authors.affiliation: Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Calle Tirant lo Blanch 7, Alfara del Patriarca, 46115 Valencia, Spain. Electronic address: alomafe@yahoo.es.
authors.forename: A
authors.initials: A
authors.lastname: Mayordomo-Febrer
authors.affiliation: Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad CEU Cardenal Herrera, Calle Tirant lo Blanch 7, Alfara del Patriarca, 46115 Valencia, Spain.
authors.forename: M
authors.initials: M
authors.lastname: López-Murcia
authors.affiliation: Unidad Central de Investigación en Medicina, Facultad de Medicina, Universidad de Valencia, Avenida Blasco Ibáñez 15, 46010 Valencia, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Morales-Tatay
authors.affiliation: Fundación de Investigación del Hospital Clínico Universitario de Valencia, Instituto de Investigación Sanitaria Clínico Valencia (INCLIVA), Avenida Blasco Ibáñez 17, 46010 Valencia, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Monleón-Salvado
authors.affiliation: Surgery-Ophthalmology Department, Faculty of Medicine, University of Valencia, Valencia, Spain; Ophthalmic Research Unit "Santiago Grisolia", Faculty of Medicine, University of Valencia, Valencia, Spain.
authors.forename: M D
authors.initials: MD
authors.lastname: Pinazo-Durán
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000869:Anterior Eye Segment / Q000378:metabolism / Q000473:pathology; D001082:Aqueous Humor / Q000187:drug effects / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D004195:Disease Models, Animal; D004596:Electroretinography; D005500:Follow-Up Studies; D005901:Glaucoma / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000633:toxicity; D007267:Injections; D007429:Intraocular Pressure; D008055:Lipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D055432:Metabolomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D008055:Lipids; D006820:Hyaluronic Acid
keywords: Aqueous humor; Glaucoma model; Metabolomics; Nuclear magnetic resonance; Rats; Sodium hyaluronate