title: A luminol electrochemiluminescence aptasensor based on glucose oxidase modified gold nanoparticles for measurement of platelet-derived growth factor BB.
abstract: A sandwich-type luminol electrochemiluminescence (ECL) aptasensor for highly sensitive and selective detection of platelet-derived growth factor BB (PDGF-BB) is fabricated. For this proposed ECL aptasensor, a multilayered AuNPs-electrochemically reduced graphene (AuNPs-EG) nanocomposite film was formed on the GCE surface as the base of the aptasensor through a co-electrodeposition method. The AuNPs-EG composites possess high conductivity to promote the electron transfer at the electrode interface and good biocompatibility and large surface area to capture large amounts of primary aptamer (Apt1), thus amplifying the detection response. Moreover, glucose oxidase (GOD) functionalized AuNPs labeled secondary aptamer (GOD-Apt2-AuNPs) was designed as the signal probe for the sandwiched aptasensor. Enhanced sensitivity was obtained by in situ generation of H2O2 from reaction between GOD and glucose and the excellent catalytic behavior of AuNPs to the ECL of the luminol-H2O2 system. Under the optimal conditions, the as-prepared ECL aptasensor exhibited excellent analytical property for the detection of PDGF-BB in the range from 1.0×10(-13) to 5.0×10(-10) mol L(-1) with a detection limit of 1.7×10(-14) mol L(-1) (S/N=3). The application of the present protocol was demonstrated by analyzing PDGF-BB in human serum and human urine samples with the recoveries from 85.0% to 110%.
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Cao
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Shi
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China.
authors.forename: Ke-Jing
authors.initials: KJ
authors.lastname: Huang
authors.affiliation: College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China. Electronic address: liuym9518@sina.com.
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Xinyang Central Hospital, Xinyang 464000, PR China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Ren
mesh_terms: D000077214:Becaplermin; D015374:Biosensing Techniques / Q000295:instrumentation*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D020574:Proto-Oncogene Proteins c-sis / Q000097:blood / Q000652:urine
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D008165:Luminol; D006046:Gold; D006108:Graphite; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
keywords: Aptasensor; Electrochemiluminescence; Glucose oxidase (GOD); Luminol; PDGF-BB