title: Inhibitory effects of biocides on transcription and protein activity of acetylcholinesterase in the intertidal copepod Tigriopus japonicus.
abstract: Acetlycholinesterase (AChE) is a serine esterase that plays an important role in the hydrolytic degradation of acetylcholine. We investigated the modulatory potential of T. japonicus-AChE (TJ-AChE) for biocide response by cloning, sequencing, and characterizing the full-length genomic DNA of the TJ-AChE1 and TJ-AChE2 genes. The deduced TJ-AChE proteins were highly conserved across species and were distinctively separated into two subtypes, AChE1 and AChE2. Each TJ-AChE protein was closely phylogenetically clustered with invertebrate AChE1 and AChE2 proteins. Transcriptional level of TJ-AChE1 was higher than TJ-AChE2 in all developmental stages. TJ-AChE1 mRNA decreased in response to five biocides (alachlor, chlorpyrifos, dimethoate, endosulfan, lindane,) but not in the molinate-exposed group. TJ-AChE2 decreased significantly only in response to chlorpyrifos and lindane. TJ-AChE enzymatic activity was significantly inhibited when exposed to alachlor, chlorpyrifos, endosulfan, or lindane for 24 h. This study elucidates potential endogenous mechanisms of biocide-induced neurotoxicity in T. japonicas.
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 440-746, South Korea.
authors.forename: Jin Wuk
authors.initials: JW
authors.lastname: Lee
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Kim
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Jeong
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Won
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D033342:Copepoda / Q000187:drug effects* / Q000201:enzymology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D010575:Pesticides / Q000494:pharmacology*; D010802:Phylogeny
chemical_list: D002800:Cholinesterase Inhibitors; D010575:Pesticides; D000110:Acetylcholinesterase