title: Expression of genes related to prostaglandin synthesis or signaling in human subcutaneous and omental adipose tissue: depot differences and modulation by adipogenesis.
abstract: OBJECTIVE
  (1) To examine depot-specific PGE2 and PGF2α release and mRNA expression of enzymes or receptors involved in PG synthesis or signaling in human adipose tissues; (2) to identify changes in expression of these transcripts through preadipocyte differentiation; and (3) to examine associations between adipose tissue mRNA expression of these transcripts and adiposity measurements.
  
  
  METHODS
  Fat samples were obtained surgically in women. PGE2 and PGF2α release by preadipocytes and adipose tissue explants was measured. Expression levels of mRNA coding for enzymes or receptors involved in PG synthesis or signaling were measured by RT-PCR.
  
  
  RESULTS
  Cultured preadipocytes and explants from omental fat released more PGE2 and PGF2α than those from the subcutaneous depot and the corresponding transcripts showed consistent depot differences. Following preadipocyte differentiation, expression of PLA2G16 and PTGER3 mRNA was significantly increased whereas COX-1, COX-2, PTGIS, and PTGES mRNA abundance were decreased in both compartments (P ≤ 0.01 for all). Transcripts that were stimulated during adipogenesis were those that correlated best with adiposity measurements.
  
  
  CONCLUSIONS
  Cells from the omental fat compartment release more PGE2 and PGF2α than those from the subcutaneous depot. Obesity modulates expression of PG-synthesizing enzymes and PG receptors which likely occurs through adipogenesis-induced changes in expression of these transcripts.
authors.affiliation: Endocrinology and Nephrology, CHU de Québec, 2705 Laurier Boulevard (R-4779), Québec, QC, Canada G1V 4G2 ; Department of Nutrition, Laval University, 2425 Rue de l'Agriculture, Québec, QC, Canada G1V 0A6.
authors.forename: Andréanne
authors.initials: A
authors.lastname: Michaud
authors.affiliation: Reproduction and Biology, CHU de Québec, 2705 Laurier Boulevard, Québec, QC, Canada G1V 4G2.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lacroix-Pépin
authors.affiliation: Endocrinology and Nephrology, CHU de Québec, 2705 Laurier Boulevard (R-4779), Québec, QC, Canada G1V 4G2.
authors.forename: Mélissa
authors.initials: M
authors.lastname: Pelletier
authors.affiliation: Gynecology Unit, CHU de Québec, 2705 Laurier Boulevard, Québec, QC, Canada G1V 4G2.
authors.forename: Marleen
authors.initials: M
authors.lastname: Daris
authors.affiliation: Department of Surgery, Institut Universitaire de Cardiologie et de Pneumologie, 2725 Chemin Sainte-Foy, Québec, QC, Canada G1V 4G5.
authors.forename: Laurent
authors.initials: L
authors.lastname: Biertho
authors.affiliation: Reproduction and Biology, CHU de Québec, 2705 Laurier Boulevard, Québec, QC, Canada G1V 4G2.
authors.forename: Michel A
authors.initials: MA
authors.lastname: Fortier
authors.affiliation: Endocrinology and Nephrology, CHU de Québec, 2705 Laurier Boulevard (R-4779), Québec, QC, Canada G1V 4G2 ; Department of Nutrition, Laval University, 2425 Rue de l'Agriculture, Québec, QC, Canada G1V 0A6.
authors.forename: André
authors.initials: A
authors.lastname: Tchernof
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D002454:Cell Differentiation; D015237:Dinoprost / Q000096:biosynthesis; D015232:Dinoprostone / Q000096:biosynthesis; D005260:Female; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D009852:Omentum / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D050151:Subcutaneous Fat / Q000378:metabolism
chemical_list: D011453:Prostaglandins; D012333:RNA, Messenger; D015237:Dinoprost; D015232:Dinoprostone