title: Methylation contributes to imbalance of PRDM1α/PRDM1bβ expression in diffuse large B-cell lymphoma.
abstract: The positive regulatory domain 1 (PRDM1) exists as two isoforms: PRDM1α and PRDM1β. The former is frequently inactivated, while the latter is overexpressed in a subset of diffuse large B-cell lymphoma (DLBCL). To investigate the possible epigenetic alteration of PRDM1α and PRDM1β expression, the methylation of these two promoter isoforms was assessed in B lymphoma cell lines and DLBCL samples. Hypomethylation of PRDM1β CpG islands was preferentially detected in lymphoma cells. However, both high and low methylation of PRDM1α CpG islands was simultaneously observed in cases of DLBCL compared with the moderate methylation of non-tumor cases. CpG 16-21-specific high methylation was correlated with low expression of PRDM1α in PRDM1β-positive DLBCL samples. Three increased and one decreased miRNAs were significantly different between cases of DLBCL and non-tumor reactive hyperplasia. Thus, our results indicate that aberrant methylation silencing of PRDM1α and hypomethylation activation of PRDM1β are frequent events in DLBCL.
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.|b Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health , Suzhou , Jiangsu Province , China.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: b Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health , Suzhou , Jiangsu Province , China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Jun-Feng
authors.initials: JF
authors.lastname: Zhu
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Yang
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhou
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Hu
authors.affiliation: a Department of Hematology , The First Affiliated Hospital of Bengbu Medical College , Bengbu , Anhui Province , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D001374:Azacitidine / Q000494:pharmacology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D018899:CpG Islands; D019175:DNA Methylation; D015536:Down-Regulation; D057890:Epigenomics; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D020868:Gene Silencing; D056426:Genetic Loci; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology*; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D000074462:Positive Regulatory Domain I-Binding Factor 1; D011401:Promoter Regions, Genetic; D020033:Protein Isoforms; D012097:Repressor Proteins / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D035683:MicroRNAs; D020033:Protein Isoforms; D012097:Repressor Proteins; C087042:PRDM1 protein, human; D000074462:Positive Regulatory Domain I-Binding Factor 1; D001374:Azacitidine
keywords: DNA methylation; PRDM1; diffuse large B-cell lymphoma; epigenetic; isoform