title: MicroRNA-1269 promotes proliferation in human hepatocellular carcinoma via downregulation of FOXO1.
abstract: BACKGROUND
  Hepatocellular carcinoma (HCC) is one of the most common malignancies and a major cause of cancer-related mortality in the world. MicroRNAs (miRNAs) are small, noncoding RNAs that play essential roles in various stages during cancer progression. The aim of the current study was to elucidate the role of miR-1269 in the pathogenesis of HCC.
  
  
  METHODS
  The expression of miR-1269 in HCC cells and tissues were determined by Real-time PCR analysis. Cell viability, colony formation and anchorage-independent growth ability assays were performed to examine cell proliferative capacity and tumorigenicity. Flow cytometry analysis was conducted to determine cell cycle progression. The expression of p21, CyclinD1, phosphorylated Rb, Rb and FOXO1 were examined by Western blotting analysis. Luciferase assay was used to determine whether FOXO1 is the direct target of miR-1269.
  
  
  RESULTS
  miR-1269 was upregulated in HCC cells and tissues. Ectopic miR-1269 expression promoted, but inhibition of miR-1269 reduced, proliferation, tumorigenicity and cell cycle progression of HCC cells. Furthermore, we demonstrated that FOXO1 was a direct target of miR-1269. Suppression of FOXO1 by miR-1269 was associated with dysregulation of p21, cyclin D1, phosphorylated Rb and Ki67 expression, thereby playing an essential role in the growth of HCC cells.
  
  
  CONCLUSIONS
  Our study indicated that overexpression of miR-1269 promotes cell proliferation in HCC through directly suppressing FOXO1, and functions as an oncomiR in HCC.
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Yang
authors.forename: Guan-Zhu
authors.initials: GZ
authors.lastname: Shen
authors.forename: Liang-Qi
authors.initials: LQ
authors.lastname: Cao
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Jiang
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Peng
authors.forename: Gang
authors.initials: G
authors.lastname: Shen
authors.forename: De
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Guangzhou, Medical University, Guangzhou 510260, China. drxueping@medmail.com.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Xue
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C000600169:MIRN1269 microRNA, human; D035683:MicroRNAs