title: Activation of Gq/11 in the mouse corpus luteum is required for parturition.
abstract: Mice with a deletion of Gα(q/11) in granulosa cells were previously shown to be subfertile. They also have a reduced ovulatory response due to a deficiency in the ability of the activated LH receptor to fully induce the granulosa cell progesterone receptor. Because this conditional deletion of Gα(q/11) will interfere with the actions of any G protein-coupled receptor that activates G(q/11) in granulosa or luteal cells, we sought to determine whether the actions of other hormones that contribute to fertility were also impaired. We focused our attention on prostaglandin F2 (PGF2)α, because this hormone is known to activate phospholipase C (a prominent Gα(q/11) effector) in luteal cells and because the action of PGF2α on luteal cells is the first step in the murine parturition pathway. Our data show that the conditional deletion of Gα(q/11) from granulosa cells prevents the ability of PGF2α to induce Akr1c18 in luteal cells. Akr1c18 codes for 20α-hydroxysteroid dehydrogenase, an enzyme that inactivates progesterone. The PGF2α-mediated induction of this enzyme towards the end of pregnancy increases the inactivation of progesterone and precipitates parturition in mice. Thus, the conditional deletion of Gαq/11 from granulosa/luteal cells prevents the progesterone withdrawal that occurs at the end of pregnancy and impairs parturition. This novel molecular defect contributes to the subfertile phenotype of the mice with a deletion of Gα(q/11) from granulosa cells.
authors.affiliation: Department of Obstetrics and Gynecology (R.M., M.A.), Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242; and Department of Pharmacology (C.W., M.A.), Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242.
authors.forename: Rachel
authors.initials: R
authors.lastname: Mejia
authors.forename: Courtney
authors.initials: C
authors.lastname: Waite
authors.forename: Mario
authors.initials: M
authors.lastname: Ascoli
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D003338:Corpus Luteum / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015237:Dinoprost / Q000378:metabolism; D005260:Female; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism*; D017353:Gene Deletion; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D036801:Parturition / Q000187:drug effects; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000494:pharmacology; D011974:Receptors, LH / Q000378:metabolism
chemical_list: D015415:Biomarkers; D006063:Chorionic Gonadotropin; D007295:Inositol Phosphates; D011974:Receptors, LH; D011374:Progesterone; D011388:Prolactin; D015237:Dinoprost; C045073:Cre recombinase; D019426:Integrases; D043802:GTP-Binding Protein alpha Subunits, Gq-G11