title: Deletion of the ORF9p acidic cluster impairs the nuclear egress of varicella-zoster virus capsids.
abstract: The protein encoded by ORF9 is essential for varicella-zoster virus (VZV) replication. Previous studies documented its presence in the trans-Golgi network and its involvement in secondary envelopment. In this work, we deleted the ORF9p acidic cluster, destroying its interaction with ORF47p, and this resulted in a nuclear accumulation of both proteins. This phenotype results in an accumulation of primary enveloped capsids in the perinuclear space, reflecting a capsid de-envelopment defect.
authors.affiliation: University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium.
authors.forename: Laura
authors.initials: L
authors.lastname: Riva
authors.affiliation: University of Liege, GIGA Neurosciences, Laboratory of Cellular and Tissular Biology, Liege, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Thiry
authors.affiliation: University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium.
authors.forename: Marielle
authors.initials: M
authors.lastname: Lebrun
authors.affiliation: University of Liege, GIGA Signal Transduction, Laboratory of Virology and Immunology, Liege, Belgium.
authors.forename: Laurent
authors.initials: L
authors.lastname: L'homme
authors.affiliation: University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium University of Liege, GIGA Signal Transduction, Laboratory of Virology and Immunology, Liege, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Piette
authors.affiliation: University of Liege, GIGA Infection Immunity and Inflammation, Laboratory of Virology and Immunology, Liege, Belgium csadzot@ulg.ac.be.
authors.forename: Catherine
authors.initials: C
authors.lastname: Sadzot-Delvaux
mesh_terms: D002213:Capsid / Q000378:metabolism*; D002467:Cell Nucleus / Q000821:virology; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000502:physiology*; D017384:Sequence Deletion; D014764:Viral Proteins / Q000235:genetics*; D057074:Virus Release; D014779:Virus Replication
chemical_list: D014764:Viral Proteins