title: The mouse hindbrain: an in vivo model to analyze developmental angiogenesis.
abstract: Angiogenesis, defined as the sprouting of new blood vessels from preexisting ones, is a biological process of great clinical relevance due to its involvement in disease as well as its therapeutic potential for revascularizing ischemic tissues. The embryonic mouse hindbrain provides an excellent model to study the molecular and cellular mechanisms of angiogenesis in vivo due the simple geometry of the hindbrain vasculature and its easy accessibility for fluorescent or histochemical staining, and for image capture and quantitation. This chapter outlines protocols for dissection, staining, and analysis of the mouse embryo hindbrain vasculature.
authors.affiliation: UCL Institute of Ophthalmology, University College London, London, UK.
authors.forename: Alice
authors.initials: A
authors.lastname: Plein
authors.forename: Christiana
authors.initials: C
authors.lastname: Ruhrberg
authors.forename: Alessandro
authors.initials: A
authors.lastname: Fantin
mesh_terms: D000818:Animals; D004210:Dissection; D005260:Female; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects; D011247:Pregnancy; D012249:Rhombencephalon / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D013194:Staining and Labeling; D013629:Tamoxifen / Q000494:pharmacology; D016707:Tissue Fixation
chemical_list: D013629:Tamoxifen