title: Functional analysis of endoplasmic reticulum glucosyltransferase (UGGT): Synthetic chemistry's initiative in glycobiology.
abstract: UGGT1 is called as a folding sensor protein that recognizes misfolded glycoproteins and selectively glucosylates high-mannose-type glycans on the proteins. However, conventional approaches using naturally occurring glycoproteins is not optimum in performing precise analysis of the unique properties of UGGT1. We have demonstrated that high-mannose-type glycans, in which various hydrophobic aglycons were introduced, act as good substrates for UGGT1 and are useful analytical tools for its characterization. Moreover, we found that UGGT2, an isoform UGGT1, is also capable of glucosylating these synthetic substrates. Our strategy stemmed on synthetic chemistry has been further strengthened by total synthesis of homogeneous glycoproteins in correctly folded as well as in intentionally misfolded forms.
authors.affiliation: Synthetic Cellular Chemistry Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; JST, ERATO, Ito Glycotrilogy Project, Wako, Saitama 351-0198, Japan. Electronic address: yukito@riken.jp.
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
authors.affiliation: JST, ERATO, Ito Glycotrilogy Project, Wako, Saitama 351-0198, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takeda
authors.affiliation: JST, ERATO, Ito Glycotrilogy Project, Wako, Saitama 351-0198, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.affiliation: Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Izumi
authors.affiliation: JST, ERATO, Ito Glycotrilogy Project, Wako, Saitama 351-0198, Japan; Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kajihara
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005964:Glucosyltransferases / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000096:biosynthesis* / Q000737:chemistry; D017510:Protein Folding
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D005964:Glucosyltransferases
keywords: Glycoprotein quality control system; Sep15; Synthetic glycoproteins; Synthetic oligosaccharides; UDP-Glc: glycoprotein glucosyltransferse