title: Characterisation of biosynthesised silver nanoparticles by scanning electrochemical microscopy (SECM) and voltammetry.
abstract: Silver nanoparticles (AgNPs) were biosynthesised by a Klebsiella oxytoca strain BAS-10, which, during its growth, is known to produce a branched exopolysaccharide (EPS). Klebsiella oxytoca cultures, treated with AgNO3 and grown under either aerobic or anaerobic conditions, produced silver nanoparticles embedded in EPS (AgNPs-EPS) containing different amounts of Ag(0) and Ag(I) forms. The average size of the AgNPs-EPS was determined by transmission electron microscopy, while the relative abundance of Ag(0)- or Ag(I)-containing AgNPs-EPS was established by scanning electrochemical microscopy (SECM). Moreover, the release of silver(I) species from the various types of AgNPs-EPS was investigated by combining SECM with anodic stripping voltammetry. These measurements allowed obtaining information on the kinetic of silver ions release from AgNPs-EPS and their concentration profiles at the substrate/water interface.
authors.affiliation: Dipartimento di Scienze Ambientali, Infomatica e Statistica, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I). Electronic address: dario.battistel@unive.it.
authors.forename: Dario
authors.initials: D
authors.lastname: Battistel
authors.affiliation: Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I).
authors.forename: Franco
authors.initials: F
authors.lastname: Baldi
authors.affiliation: Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I).
authors.forename: Michele
authors.initials: M
authors.lastname: Gallo
authors.affiliation: Dipartimento Scienze della Vita, Siena University, via Mattioli 4, 53100 Siena (I).
authors.forename: Claudia
authors.initials: C
authors.lastname: Faleri
authors.affiliation: Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari Venice. Calle Larga Santa Marta 2137. 30127 Venice, (I). Electronic address: sig@unive.it.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Daniele
mesh_terms: D001692:Biological Transport; D055664:Electrochemical Techniques; D041121:Klebsiella oxytoca / Q000737:chemistry / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D064347:Microscopy, Electrochemical, Scanning; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry*; D012834:Silver / Q000737:chemistry* / Q000378:metabolism; D012835:Silver Nitrate / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011135:Polysaccharides, Bacterial; D012834:Silver; D012835:Silver Nitrate
keywords: Anodic stripping voltammetry; Bacteria; Biosynthesis; Scanning electrochemical microscopy; Silver nanoparticles