title: Spore inactivation and DPA release in Alicyclobacillus acidoterrestris under different stress conditions.
abstract: This paper reports on the inactivation of spores of 5 strains of Alicyclobacillus acidoterrestris under different stress conditions (acidic and alkaline pH, high temperature, addition of lysozyme, hydrogen peroxide and p-coumaric acid). The research was divided into two different steps; first, each stress was studied alone, thus pointing out a partial uncoupling between spore inactivation and DPA release, as H2O2 reduced spore level below the detection but it did not cause the release of DPA. A partial correlation was found only for acidic and alkaline pH. 2nd step was focused on the combination of pH, temperature and H2O2 through a factorial design; experiments were performed on both fresh and 4 month-old spores and pinpointed a different trend for DPA release as a function of spore age.
authors.affiliation: Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli 25, 71122, Foggia, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Bevilacqua
authors.affiliation: Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli 25, 71122, Foggia, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Ciuffreda
authors.affiliation: Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli 25, 71122, Foggia, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Sinigaglia
authors.affiliation: Department of the Science of Agriculture, Food and Environment (SAFE), University of Foggia, Via Napoli 25, 71122, Foggia, Italy. Electronic address: mariarosaria.corbo@unifg.it.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Corbo
mesh_terms: D056450:Alicyclobacillus / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050296:Microbial Viability; D010848:Picolinic Acids / Q000378:metabolism*; D013171:Spores, Bacterial / Q000254:growth & development* / Q000378:metabolism / Q000502:physiology; D013312:Stress, Physiological; D013696:Temperature
chemical_list: D010848:Picolinic Acids; D006861:Hydrogen Peroxide; C004860:dipicolinic acid
keywords: Alicyclobacillus; DPA release; Inactivation; Process optimization; Spore