title: Naturally occurring entomopathogenic fungi infecting stored grain insect species in Punjab, Pakistan.
abstract: The occurrence of entomopathogenic fungi isolated from stored grain insect pests sampled from various geographical regions of Punjab, Pakistan, was investigated. In total, 25,720 insects from six different species were evaluated, and 195 isolates from 24 different fungal species were recovered. These included the Ascomycetes Beauveria bassiana sensu lato (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae), Metarhizium anisopliae sensu lato (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae), Purpureocillium lilacinum (Thorn) Samson (Hypocreales: Ophiocordycipitaceae), and Lecanicillium attenuatum (Zare and W. Gams) (Hypocreales: Clavicipitaceae). The cadavers of red flour beetle Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) were significantly infected with the fungi followed by rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae), lesser grain borer Rhyzopertha dominica (F.) (Coleoptera: Bostrichidae), rusty grain beetle Cryptolestes ferrugineus (Stephens) (Coleoptera: Cucujidae), and cowpea weevil Callosobruchus maculatus (F.) (Coleoptera: Bruchidae); however, the least were recovered from khapra beetle Trogoderma granarium (Everts) (Coleoptera: Dermestidae). The geographical attributes (altitude, longitude, and latitude) greatly influenced the occurrence of entomopathogenic fungi with highest number of isolates found from >400 (m) altitude, 33°-34' N latitude, and 73°-74' E longitude. The findings of the current surveys clearly indicated that the entomopathogenic fungi are widely distributed in the insect cadavers, which may later be used in successful Integrated Pest Management programs.
authors.affiliation: Department of Entomology, University of Agriculture, Faisalabad, Pakistan waqaswakeel@hotmail.com.
authors.forename: Waqas
authors.initials: W
authors.lastname: Wakil
authors.affiliation: Department of Plant Pathology, University College of Agriculture, University of Sargodha, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Usman Ghazanfar
authors.affiliation: Department of Entomology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yasin
mesh_terms: D000818:Animals; D061046:Biological Control Agents; D001517:Coleoptera / Q000254:growth & development / Q000382:microbiology*; D002523:Edible Grain; D006999:Hypocreales / Q000302:isolation & purification* / Q000502:physiology; D007814:Larva / Q000254:growth & development / Q000382:microbiology; D010154:Pakistan
chemical_list: D061046:Biological Control Agents
keywords: entomopathogenic fungi; localities; occurrence; stored grain insects; virulence