title: Thioredoxin reductase activity may be more important than GSH level in protecting human lens epithelial cells against UVA light.
abstract: This study compares the abilities of the glutathione (GSH) and thioredoxin (Trx) antioxidant systems in defending cultured human lens epithelial cells (LECs) against UVA light. Levels of GSH were depleted with either L-buthionine-(S,R)-sulfoximine (BSO) or 1-chloro-2,4-dinitrobenzene (CDNB). CDNB treatment also inhibited the activity of thioredoxin reductase (TrxR). Two levels of O2 , 3% and 20%, were employed during a 1 h exposure of the cells to 25 J cm(-2) of UVA radiation (338-400 nm wavelength, peak at 365 nm). Inhibition of TrxR activity by CDNB, combined with exposure to UVA light, produced a substantial loss of LECs and cell damage, with the effects being considerably more severe at 20% O2 compared to 3%. In contrast, depletion of GSH by BSO, combined with exposure to UVA light, produced only a slight cell loss, with no apparent morphological effects. Catalase was highly sensitive to UVA-induced inactivation, but was not essential for protection. Although UVA light presented a challenge for the lens epithelium, it was well tolerated under normal conditions. The results demonstrate an important role for TrxR activity in defending the lens epithelium against UVA light, possibly related to the ability of the Trx system to assist DNA synthesis following UVA-induced cell damage.
authors.affiliation: Eye Research Institute, Oakland University, Rochester, MI.
authors.forename: Vanita A
authors.initials: VA
authors.lastname: Padgaonkar
authors.forename: Victor R
authors.initials: VR
authors.lastname: Leverenz
authors.forename: Aparna V
authors.initials: AV
authors.lastname: Bhat
authors.forename: Sara E
authors.initials: SE
authors.lastname: Pelliccia
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Giblin
mesh_terms: D002374:Catalase / Q000378:metabolism; D002452:Cell Count; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004137:Dinitrochlorobenzene / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D005978:Glutathione / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D018384:Oxidative Stress; D010100:Oxygen / Q000494:pharmacology; D054481:Thioredoxin Reductase 1 / Q000378:metabolism*; D013879:Thioredoxins / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D004137:Dinitrochlorobenzene; C491203:TXN protein, human; C065566:buthionine sulfoximine ethyl ester; D008717:Methionine Sulfoximine; D013879:Thioredoxins; D002374:Catalase; C516124:TXNRD1 protein, human; D054481:Thioredoxin Reductase 1; D005978:Glutathione; D010100:Oxygen