title: Single vial freeze-dried TRODAT-1 kit: preparation and demonstration of clinical efficacy of [(99m)Tc]TRODAT-1 in Indian scenario.
abstract: A single vial freeze-dried kit formulation for preparation of three patients' dose of [(99m)Tc]TRODAT-1 has been developed for early diagnosis of Parkinson's disease (PD). Kits were evaluated to ascertain the purity, stability and batch to batch variations. Preclinical evaluation was carried out in laboratory animals and clinical imaging was performed in human patients with PD. The labeling yield and purity of [(99m)Tc]TRODAT-1 was >90%. Swiss mice showed retention of [(99m)Tc]TRODAT-1 in the mid brain region. Clinical studies showed decreased striatal uptake with increasing severity of PD.
authors.affiliation: Isotope Applications & Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Korde
authors.affiliation: Isotope Applications & Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Mythili
authors.initials: M
authors.lastname: Kameswaran
authors.affiliation: Isotope Applications & Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Drishty
authors.initials: D
authors.lastname: Satpati
authors.affiliation: Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.
authors.forename: Haladhar
authors.initials: H
authors.lastname: Dev Sarma
authors.affiliation: Isotope Applications & Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India.
authors.forename: Sharmila
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Kovai Medical Center and Hospital, Coimbatore, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Shinto
authors.affiliation: Kovai Medical Center and Hospital, Coimbatore, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Kamaleshwaran
authors.affiliation: Isotope Applications & Radiopharmaceuticals Division, Bhabha Atomic Research Centre, Mumbai 400085, India. Electronic address: grace@barc.gov.in.
authors.forename: Grace
authors.initials: G
authors.lastname: Samuel
mesh_terms: D000368:Aged; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism; D016022:Case-Control Studies; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D042241:Early Diagnosis; D005260:Female; D005612:Freeze Drying; D006801:Humans; D007194:India; D008297:Male; D051379:Mice; D008875:Middle Aged; D015609:Organotechnetium Compounds / Q000302:isolation & purification / Q000592:standards; D010300:Parkinson Disease / Q000000981:diagnostic imaging* / Q000378:metabolism; D011786:Quality Control; D019275:Radiopharmaceuticals / Q000302:isolation & purification / Q000592:standards; D051381:Rats; D017208:Rats, Wistar; D015899:Tomography, Emission-Computed, Single-Photon; D014326:Tropanes / Q000302:isolation & purification / Q000592:standards
chemical_list: D050483:Dopamine Plasma Membrane Transport Proteins; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; D014326:Tropanes; C104196:technetium Tc 99m TRODAT-1
keywords: Freeze-dried cold kit; Parkinson׳s disease (PD); [(99m)Tc]TRODAT-1