title: Comparison of docosahexaenoic acid uptake in murine cardiomyocyte culture and tissue: significance to physiologically relevant studies.
abstract: Long-chain n-3 (or omega 3) fatty acids, namely docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) have been attributed cardioprotective properties. In this study, we evaluated the incorporation of DHA into cardiomyocytes and the shift in the omega 3/omega 6 ratio after supplementation of primary cardiomyocyte culture. Results are compared with atrial tissue concentrations attained after prolonged feeding of rats. The major difference between in vitro vs. in vivo supplementation is the paradoxical accumulation of arachidonic acid in cultured cardiomyocyte. However, this increase does not give rise to a higher PGE2 production after cellular stimulation, as compared with controls, possibly because of the associated inhibition of sPLA2 by DHA. Notably, in vitro supplementations with DHA 10 to 25μM approximate in vivo pharmacological treatments.
authors.affiliation: Laboratory of Mass Spectrometry, ERL INSERM U 1057/UMR 7203, Université Pierre et Marie Curie, Paris, France.
authors.forename: Antonin
authors.initials: A
authors.lastname: Lamaziere
authors.affiliation: UPMC Univ Paris 06, CNRS UMR8256/INSERM ERL U1164, Institut de Biologie Paris Seine, Paris, France.
authors.forename: Doriane
authors.initials: D
authors.lastname: Richard
authors.affiliation: UPMC Univ Paris 06, CNRS UMR8256/INSERM ERL U1164, Institut de Biologie Paris Seine, Paris, France.
authors.forename: Pedro
authors.initials: P
authors.lastname: Bausero
authors.affiliation: UPMC Univ Paris 06, CNRS UMR8256/INSERM ERL U1164, Institut de Biologie Paris Seine, Paris, France.
authors.forename: Ullah
authors.initials: U
authors.lastname: Barbe
authors.affiliation: UPMC Univ Paris 06, CNRS UMR8256/INSERM ERL U1164, Institut de Biologie Paris Seine, Paris, France.
authors.forename: Kaouthar
authors.initials: K
authors.lastname: Kefi
authors.affiliation: Laboratory of Mass Spectrometry, ERL INSERM U 1057/UMR 7203, Université Pierre et Marie Curie, Paris, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Wolf
authors.affiliation: Laboratory of Functional Foods, Madrid Institute for Advanced Studies (IMDEA) - Food, Madrid, Spain; Department of Molecular Medicine, University of Padua, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Visioli
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019587:Dietary Supplements; D004281:Docosahexaenoic Acids / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D043371:Fatty Acids, Omega-6 / Q000378:metabolism; D008297:Male; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D043371:Fatty Acids, Omega-6; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid
keywords: Arachidonic acid; Cardiomyocytes; Cardiovascular disease; Docosahexaenoic acid; Mass spectrometry; Phospholipids