title: Batf3 deficiency is not critical for the generation of CD8α⁺ dendritic cells.
abstract: Recently, we have reported that CD8α(+) DCs, rather than CD8(+) T cells, are involved in the establishment and maintenance of HSV-1 latency in the trigeminal ganglia (TG) of ocularly infected mice. In the current study, we investigated whether similar results can be obtained using Batf3(-/-) mice that previously were reported to lack CD8α(+) DCs. However, our results demonstrate that Batf3(-/-) mice, without any known infection, express CD8α(+) DCs. Consequently, due to the presence of CD8α(+) DCs, no differences were detected in the level of HSV-1 latency between Batf3(-/-) mice compared with wild type control mice.
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Kevin R
authors.initials: KR
authors.lastname: Mott
authors.affiliation: Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Hadi
authors.initials: H
authors.lastname: Maazi
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Sariah J
authors.initials: SJ
authors.lastname: Allen
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Mandana
authors.initials: M
authors.lastname: Zandian
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Harry
authors.initials: H
authors.lastname: Matundan
authors.affiliation: Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Yasamin N
authors.initials: YN
authors.lastname: Ghiasi
authors.affiliation: Division of Cardiology, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Behrooz G
authors.initials: BG
authors.lastname: Sharifi
authors.affiliation: Inflammatory Bowel & Immunobiology Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: David
authors.initials: D
authors.lastname: Underhill
authors.affiliation: Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
authors.forename: Omid
authors.initials: O
authors.lastname: Akbari
authors.affiliation: Center for Neurobiology and Vaccine Development, Ophthalmology Research, Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Electronic address: ghiasih@cshs.org.
authors.forename: Homayon
authors.initials: H
authors.lastname: Ghiasi
mesh_terms: D000818:Animals; D050976:Basic-Leucine Zipper Transcription Factors / Q000172:deficiency*; D016827:CD8 Antigens / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism*; D004195:Disease Models, Animal; D055786:Gene Knockout Techniques; D006561:Herpes Simplex / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology; D007150:Immunohistochemistry; D016130:Immunophenotyping; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D011817:Rabbits; D012097:Repressor Proteins / Q000172:deficiency*
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D016827:CD8 Antigens; C409469:CD8 antigen, alpha chain; D012097:Repressor Proteins; C533289:SNFT protein, mouse
keywords: Compensation; Dendritic cells; HSV-1; Knockout; Latency