title: Role of protein environment and bioactive polymer grafting in the S. epidermidis response to titanium alloy for biomedical applications.
abstract: Joint implant-related infections, namely by Staphylococci, are a worldwide problem, whose consequences are dramatic. Various methods are studied to fight against these infections. Here, the proposed solution consists in grafting a bioactive polymer on joint implant surfaces in order to allow the control of the interactions with the living system. In this study, sodium styrene sulfonate, bearing sulfonate groups, was grafted on the surface of titanium alloys. Scanning Electron Microscopy, colorimetric method, Fourier-transformed infrared spectroscopy and contact angle measurements were applied to characterize the surfaces. Bacterial adhesion studies were studied on poly(sodium styrene sulfonate) grafted Ti6Al4V and Ti6Al4V surfaces previously adsorbed by proteins involved in the bacteria adhesion process. Fibrinogen and fibronectin were demonstrated to increase staphylococcal adhesion on Ti6Al4V surfaces. Ti6Al4V grafted sodium styrene sulfonate surfaces inhibited the adhesion of Staphylococcus epidermidis in 37% and 13% on pre-adsorbed surfaces with fibrinogen and fibronectin, respectively. The mechanism of the observed inhibiting bacteria adhesion properties is related to the differences of proteic conformations induced by poly(sodium styrene sulfonate) grafting.
authors.affiliation: INEB - Instituto de Engenharia Biomédica, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal; Faculdade de Engenharia da Universidade do Porto (FEUP), Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal. Electronic address: dfmvasconcelos@gmail.com.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Vasconcelos
authors.affiliation: Laboratory of Biomaterials and Specialty Polymers (LBPS-CSPBAT CNRS UMR 7244), Institut Galilée, Université Paris XIII, Sorbonne Cité, 93430 Villetaneuse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Falentin-Daudré
authors.affiliation: CERAVER, 69, rue de la Belle Etoile, 95957 Roissy Cedex, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Blanquaert
authors.affiliation: Diaxonhit, 63-65 Boulevard Massena, 75013, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Thomas
authors.affiliation: INEB - Instituto de Engenharia Biomédica, Universidade do Porto, R. Campo Alegre 823, 4150-180 Porto, Portugal; Faculdade de Engenharia da Universidade do Porto (FEUP), Porto, Portugal; Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal.
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Granja
authors.affiliation: Laboratory of Biomaterials and Specialty Polymers (LBPS-CSPBAT CNRS UMR 7244), Institut Galilée, Université Paris XIII, Sorbonne Cité, 93430 Villetaneuse, France. Electronic address: veronique.migonney@univ-paris13.fr.
authors.forename: Veronique
authors.initials: V
authors.lastname: Migonney
mesh_terms: D000497:Alloys; D001422:Bacterial Adhesion / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013451:Sulfonic Acids / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011108:Polymers; D013451:Sulfonic Acids; C031462:titanium alloy (TiAl6V4); D005340:Fibrinogen; D014025:Titanium; C077114:styrenesulfonic acid polymer
keywords: Bacterial adhesion; Bioactive polymer; Poly(sodium styrene sulfonate) (polyNaSS); Protein adsorption; Surface modification; Titanium alloy