title: Physiological response of Clostridium ljungdahlii DSM 13528 of ethanol production under different fermentation conditions.
abstract: In this study, cell growth, gene expression and ethanol production were monitored under different fermentation conditions. Like its heterotrophical ABE-producing relatives, a switch from acidogenesis to solventogenesis of Clostridium ljungdahlii during the autotrophic fermentation with CO/CO2 could be observed, which occurred surprisingly in the late-log phase rather than in the transition phase. The gene expression profiles indicated that aor1, one of the putative aldehyde oxidoreductases in its genome played a critical role in the formation of ethanol, and its transcription could be induced by external acids. Moreover, a low amount of CaCO3 was proved to have positive influences on the cell density and substrate utilization, followed by an increase of over 40% ethanol and 30% acetate formation.
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, China; University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China.
authors.forename: Bin-Tao
authors.initials: BT
authors.lastname: Xie
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, China.
authors.forename: Zi-Yong
authors.initials: ZY
authors.lastname: Liu
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, China.
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Songling Road 189, Qingdao 266101, China. Electronic address: xiaohuac@yahoo.com.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Chen
mesh_terms: D000143:Acids / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D002119:Calcium Carbonate / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D002248:Carbon Monoxide / Q000378:metabolism; D003013:Clostridium / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects
chemical_list: D000143:Acids; D002245:Carbon Dioxide; D000431:Ethanol; D002248:Carbon Monoxide; D002119:Calcium Carbonate
keywords: Acid induction; CO/CO(2) fermentation; CaCO(3) effect; Clostridium ljungdahlii; Ethanol synthesis