title: Achieving direct electrochemistry of glucose oxidase by one step electrochemical reduction of graphene oxide and its use in glucose sensing.
abstract: In this paper, the direct electrochemistry of glucose oxidase (GOD) was accomplished at a glassy carbon electrode modified with electrochemically reduced graphene oxide/sodium dodecyl sulfate (GCE/ERGO/SDS). A pair of reversible peaks is exhibited on GCE/ERGO/SDS/GOD by cyclic voltammetry. The peak-to-peak potential separation of immobilized GOD is 28 mV in 0.1 M phosphate buffer solution (pH7.0) with a scan rate of 50 mV/s. The average surface coverage is 2.62×10(-10) mol cm(-2). The resulting biosensor exhibited a good response to glucose with linear range from 1 to 8 mM (R(2)=0.9878), good reproducibility and detection limit of 40.8 μM. The results from the biosensor were similar (±5%) to those obtained from the clinical analyzer.
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shamsipur
authors.affiliation: Department of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mahmoud.tabrizi@gmail.com.
authors.forename: Mahmoud Amouzadeh
authors.initials: MA
authors.lastname: Tabrizi
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D010087:Oxides / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D010087:Oxides; D012967:Sodium Dodecyl Sulfate; D002244:Carbon; D006108:Graphite; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Direct electrochemistry; Electrochemically reduced graphene oxide; Glucose; Glucose oxidase; Surfactant