title: Characterization and wound healing property of collagen-chitosan film from Sepia kobiensis (Hoyle, 1885).
abstract: The present findings were aimed to develop an effective form of collagen-chitosan film to investigate the wound healing property in Wistar rats. Primarily, the acid-soluble collagen (ASC) isolated, structurally and physicochemically characterized. FT-IR and 1H NMR spectra were confirmed the collagen peptide crosslinks. The molecular weight of ASC subunits was about 86 kDa and 79 kDa, the proline and hydroxyproline content was 13.15% and 7.55%, respectively. In addition, collagen-chitosan films were successfully prepared in a controlled environment and characterized by FT-IR and DSC. In wound healing, the experimental animal model showed the increased wound size reduction in the CChF treated rats (96.25%) when compared to control rats (33.75%) followed by CF treated rats (65%) and ChF (55%). The histological results well supported these findings. It can be concluded that a better and faster epithelization was observed in the CChF group compared to the other groups.
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India; Institute of Marine Microbes and Ecospheres, State Key Laboratory of Marine Environmental Science Xiamen University, Xiamen 361 005, Fujian, China. Electronic address: ramkanth281@gmail.com.
authors.forename: Pasiyappazham
authors.initials: P
authors.lastname: Ramasamy
authors.affiliation: Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India.
authors.forename: Annaian
authors.initials: A
authors.lastname: Shanmugam
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D008297:Male; D023421:Models, Animal; D019906:Nuclear Magnetic Resonance, Biomolecular; D051381:Rats; D049833:Sepia / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013816:Thermodynamics; D014945:Wound Healing
chemical_list: D003094:Collagen; D048271:Chitosan
keywords: Cephalopods; Chitosan; Collagen; Cuttlebone; Wound healing