title: Chemical constituents from Licania cruegeriana and their cardiovascular and antiplatelet effects.
abstract: Three new lupane-type triterpenoids: 6β,30-dihydroxybetulinic acid glucopyranosyl ester (4), 6β,30-dihydroxybetulinic acid (5) and 6β-hydroxybetulinic acid (6), were isolated from Licania cruegeriana Urb. along with six known compounds. Their structures were elucidated on the basis of spectroscopic methods, including IR, ESIMS, 1D- and 2D-NMR experiments, as well as by comparison of their spectral data with those of related compounds. All compounds were evaluated in vivo for their effects on the mean arterial blood pressure (MABP) and heart rate (HR) of spontaneously hypertensive rats (SHR) and also in vitro for their capacity to inhibit the human platelet aggregation. None of the isolated flavonoids 1-3 showed cardiovascular effects on SHR and among the isolated triterpenoids 4-9 only 5 and 6 produced a significant reduction in MABP (60.1% and 17.2%, respectively) and an elevation in HR (11.0% and 41.2%, respectively). Compounds 3, 4, 5 and 6 were able to inhibit human platelet aggregation induced by ADP, collagen and arachidonic acid with different selectivity profiles.
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. oestrada@ivic.gob.ve.
authors.forename: Omar
authors.initials: O
authors.lastname: Estrada
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. wcontreras@ivic.gob.ve.
authors.forename: Whendy
authors.initials: W
authors.lastname: Contreras
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. gacha@ivic.gob.ve.
authors.forename: Giovana
authors.initials: G
authors.lastname: Acha
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. elucena@ivic.gob.ve.
authors.forename: Eva
authors.initials: E
authors.lastname: Lucena
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. wventurini@ivic.gob.ve.
authors.forename: Whitney
authors.initials: W
authors.lastname: Venturini
authors.affiliation: Facultad de Agronomía, Universidad Central de Venezuela, Maracay 2101, Venezuela. alfonosocardozo@gmail.com.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Cardozo
authors.affiliation: Centro de Biofisica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe 1020-A, Venezuela. calvarad@ivic.gob.ve.
authors.forename: Claudia
authors.initials: C
authors.lastname: Alvarado-Castillo
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D062186:Arterial Pressure / Q000187:drug effects; D045469:Chrysobalanaceae / Q000737:chemistry*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D011918:Rats, Inbred SHR; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000959:Antihypertensive Agents; D005419:Flavonoids; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D014315:Triterpenes