title: The LEP G-2548A gene polymorphism is associated with age at menarche and breast cancer susceptibility.
abstract: Leptin is an adipocytokine made by fat cells and plays a key role in proliferation, cell survival, migration and immune response. It has a powerful effect on the initiation of puberty and in determining age at menarche. The current study is the first investigation to examine the effect of G-2548A leptin gene polymorphism on the age at menarche and breast cancer susceptibility. This case-control study was performed on 203 patients with breast cancer and 171 healthy women. The leptin genotypes were determined using the PCR-RFLP method and age at menarche was obtained by questionnaires. There was a significant difference between the leptin G-2548A genotypes between case and control groups (P<0.05). AA genotype is significantly higher in patients compared to the controls. Furthermore, women carrying the AA genotype had a significantly younger age at menarche (12.47 years) than women with the AG (12.94 years) and GG (13.47 years) genotypes. Also, we found that the AA genotype frequency in women with age at menarche <13 years was higher than in women with age at menarche ≥13 years (OR: 3.4, 95% CI: 1.7-6.7, P: 0.001). In conclusion, the G-2548A leptin gene polymorphism has an important role in the onset of menarche and breast cancer susceptibility.
authors.affiliation: Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran; Yong Researchers and Elite Club, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Rostami
authors.affiliation: Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran; Yong Researchers and Elite Club, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran. Electronic address: Kohan@iaua.ac.ir.
authors.forename: Leila
authors.initials: L
authors.lastname: Kohan
authors.affiliation: Colorectal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mohammadianpanah
mesh_terms: D000293:Adolescent; D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D020738:Leptin / Q000235:genetics*; D008572:Menarche / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D020738:Leptin
keywords: Breast cancer; Genetic polymorphism; Leptin; Menarche