title: Quantitative assessment of moniliformin in cereals via alternative precipitation pathways, aided by LC-LIT-MS and LC-Q-TOF-MS.
abstract: The availability of a simple chemical precipitation workflow aided by targeted and untargeted mass spectrometry would provide an accurate diagnostic platform for the direct determination of moniliformin in cereals for food safety control. In-house method validation was performed at six concentration levels of 8, 40, 80, 200, 400, and 600 ng g(-1) in cereal flours of wheat, corn, rye, oats and barley. Spiking experiments were made at three concentration levels of 20, 40 and 100 ng g(-1). Protein precipitation and "PHREE" column cleanup strategy provided recoveries of 81-108% for all cereals matrices using external calibrants. "PHREE" purification provided significant (p < 0.05) ion signal enhancement reduction advantage for all matrices except corn flour. Moniliformin underwent significant (p < 0.05) degradation over 2 weeks when prepared in acidified water. A simple, low-cost and fit-for-purpose procedure for the identification and quantitation of moniliformin in cereals becomes available to support prospective regulatory function.
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore. Electronic address: Jeff_lim@hsa.gov.sg.
authors.forename: Chee Wei
authors.initials: CW
authors.lastname: Lim
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.
authors.forename: Kit Yee
authors.initials: KY
authors.lastname: Lai
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.
authors.forename: Jie Fang
authors.initials: JF
authors.lastname: Yeo
authors.affiliation: AB SCIEX (Distribution), 10 Biopolis Road, #03-06, Chromos, Singapore 138670, Singapore.
authors.forename: Siew Hoon
authors.initials: SH
authors.lastname: Tai
authors.affiliation: Food Safety Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, Singapore 169078, Singapore.
authors.forename: Sheot Harn
authors.initials: SH
authors.lastname: Chan
mesh_terms: D018554:Avena / Q000737:chemistry; D002853:Chromatography, Liquid; D003503:Cyclobutanes / Q000032:analysis*; D002523:Edible Grain / Q000737:chemistry*; D005433:Flour / Q000032:analysis; D001467:Hordeum / Q000737:chemistry; D057230:Limit of Detection; D015203:Reproducibility of Results; D012434:Secale / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry
chemical_list: D003503:Cyclobutanes; C008539:moniliformin
keywords: Cereals; Diagnostic; Mass spectrometry; Moniliformin; PHREE purification