title: An enzyme-linked immunosorbent assay for detection of pyrene and related polycyclic aromatic hydrocarbons.
abstract: Polycyclic aromatic hydrocarbons (PAHs) can form DNA-binding compounds that show genotoxicity and carcinogenicity. Pyrene, as a PAH, was covalently linked to carrier protein bovine serum albumin and ovalbumin. A monoclonal antibody (McAb) was produced that showed high cross-reactivity values with chrysene (169.73%), benzo[a]pyrene (693.34%), benzo[a]anthracene (16.36%), and indeno[1,2,3-cd]pyrene (40.96%) and showed no significant cross-reactivity values with other homologues (<0.1%). A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of pyrene and some homologues in water samples. The detection limit of the assay was 65.08 pg ml(-1). The average recoveries of PAHs from tap water, lake water, and mineral water were 99.13, 99.74, and 99.19%, respectively, indicating that matrices of water samples do not interfere with the assay. The results demonstrated that the developed ELISA seems to be a potential method for monitoring of pyrene and some homologous PAHs in water samples.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Meng
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China. Electronic address: zhouyu69@sina.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: L
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: B
authors.initials: B
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: N N
authors.initials: NN
authors.lastname: Wang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: P
authors.initials: P
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: H L
authors.initials: HL
authors.lastname: Ren
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Z S
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: X R
authors.initials: XR
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002138:Calibration; D002417:Cattle; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D010047:Ovalbumin / Q000737:chemistry; D011721:Pyrenes / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D000911:Antibodies, Monoclonal; D011721:Pyrenes; D014867:Water; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; C030984:pyrene
keywords: ELISA; Monoclonal antibody; Pyrene; Water