title: Role of endogenous basic fibroblast growth factor in stem cells isolated from human exfoliated deciduous teeth.
abstract: OBJECTIVE
  This study aimed to investigate the role of endogenous basic fibroblast growth factor (bFGF) in stem cells isolated from human exfoliated deciduous teeth.
  
  
  METHODS
  Cells were isolated from dental pulp tissues of human exfoliated deciduous teeth. The expression of stem cell markers was determined using conventional semi-quantitative polymerase chain reaction (PCR) and flow cytometry. The multipotential differentiation ability was also examined. The lentiviral shRNA or fibroblast growth factor receptor (FGFR) inhibitor was employed to inhibit bFGF mRNA expression and signal transduction, respectively. The colony formation ability was determined by low-density cell seeding protocol. The mRNA expression was evaluated using real-time quantitative PCR. The osteogenic differentiation was examined using alkaline phosphatase enzymatic activity assay and alizarin red staining.
  
  
  RESULTS
  The results demonstrated that the cells isolated from human exfoliated deciduous teeth (SHEDs) exhibited stem cell characteristics, regarding marker expression and multipotential differentiation ability (osteogenic, adipogenic, and neurogenic lineage). The sh-bFGF transduced SHEDs had lower colony forming unit and higher mineralization than those of the control. Similarly, the decrease of colony number and the increase of mineral deposition were noted upon exposing cells to FGFR chemical inhibitor.
  
  
  CONCLUSIONS
  These results imply that the endogenous bFGF may participate in the colony formation and osteogenic differentiation ability. In addition, the inhibition of bFGF signalling may be useful to enhance osteogenic differentiation of stem cells.
authors.affiliation: Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: p_now@hotmail.com.
authors.forename: Nunthawan
authors.initials: N
authors.lastname: Nowwarote
authors.affiliation: Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: prasit215@gmail.com.
authors.forename: Prasit
authors.initials: P
authors.lastname: Pavasant
authors.affiliation: Research Unit of Mineralized Tissue, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand; Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: thanaphum.o@chula.ac.th.
authors.forename: Thanaphum
authors.initials: T
authors.lastname: Osathanon
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002648:Child; D016222:Fibroblast Growth Factor 2 / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D010012:Osteogenesis / Q000502:physiology; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D014094:Tooth, Deciduous / Q000166:cytology*
chemical_list: D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2; D000469:Alkaline Phosphatase
keywords: Basic fibroblast growth factor; Osteogenic differentiation; Proliferation; Stem cells isolated from human exfoliated deciduous teeth