title: Mucoadhesive electrospun chitosan-based nanofibre mats for dental caries prevention.
abstract: The mucoadhesive electrospun nanofibre mats were developed using chitosan (CS) and thiolated chitosan (CS-SH) as mucoadhesive polymers. Garcinia mangostana (GM) extract was incorporated into nanofibre mats. The antibacterial activity in the single and combined agents was evaluated against dental caries pathogens. The morphology of mats was observed using SEM. The mats were evaluated for GM extract amount, mucoadhesion, in vitro release, antibacterial activity and cytotoxicity. The mucoadhesion and antibacterial activity were determined in healthy human volunteers. The prepared mats were in nanoscale with good physical and mucoadhesive properties. The CS-SH caused the higher mucoadhesion. All mats rapidly released active substances, which had the synergistic antibacterial activity. In addition, the reduction of bacteria and good mucoadhesion in the oral cavity occurred without cytotoxicity. The results suggest that mats have the potential to be mucoadhesive dosage forms to maintain oral hygiene by reducing the bacterial growth that causes the dental caries.
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Wipada
authors.initials: W
authors.lastname: Samprasit
authors.affiliation: Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand.
authors.forename: Ruchadaporn
authors.initials: R
authors.lastname: Kaomongkolgit
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Monrudee
authors.initials: M
authors.lastname: Sukma
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Theerasak
authors.initials: T
authors.lastname: Rojanarata
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand.
authors.forename: Tanasait
authors.initials: T
authors.lastname: Ngawhirunpat
authors.affiliation: Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand. Electronic address: praneet@su.ac.th.
authors.forename: Praneet
authors.initials: P
authors.lastname: Opanasopit
mesh_terms: D000269:Adhesives / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D003731:Dental Caries / Q000382:microbiology / Q000517:prevention & control*; D004492:Edetic Acid / Q000737:chemistry; D004560:Electricity; D029762:Garcinia mangostana / Q000737:chemistry; D006801:Humans; D009061:Mouth Mucosa; D057139:Nanofibers / Q000737:chemistry*; D036103:Nanotechnology; D013295:Streptococcus mutans / Q000187:drug effects; D013298:Streptococcus sanguis / Q000187:drug effects
chemical_list: D000269:Adhesives; D000900:Anti-Bacterial Agents; D048271:Chitosan; D004492:Edetic Acid
keywords: Dental caries; Electrospinning; Mucoadhesion; Nanofibre mats; Thiolated chitosan