title: Isolation and characterization of microsatellite loci from the tick Amblyomma aureolatum (Acari: Ixodidae).
abstract: Amblyomma aureolatum (Pallas) is the main vector of the bacterium Rickettsia rickettsii, the etiological agent of Brazilian spotted fever. This disease is the most lethal human spotted fever rickettsiosis in the world. Microsatellite loci were isolated from a dinucleotide-enriched library produced from A. aureolatum sampled in Southeastern Brazil. Eight polymorphic microsatellites were further characterized among 38 individuals sampled from São Paulo metropolitan region. The number of observed alleles ranged from 2 to 9, observed heterozygosity was 0.184-0.647, and expected heterozygosity was 0.251-0.747. Cross-species amplifications suggested that these loci will be useful for other Amblyomma species.
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil mogrzewalska@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Ogrzewalska
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz" da Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Bajay
authors.affiliation: Departamento de Genética e Evolução e Bioagentes, Universidade de Campinas, Campinas, SP, Brasil.
authors.forename: K
authors.initials: K
authors.lastname: Schwarcz
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz" da Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: S K
authors.initials: SK
authors.lastname: Bajay
authors.affiliation: Laboratório de Genética e Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, Brasil.
authors.forename: M P C
authors.initials: MP
authors.lastname: Telles
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz" da Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Pólo Centro-Sul, Agência Paulista de Tecnologia dos Agronegócios, Campinas, SP, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Superintendência de Controle de Endemias, São Paulo, SP, Brasil.
authors.forename: A
authors.initials: A
authors.lastname: Pinter
authors.affiliation: Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Labruna
mesh_terms: D000818:Animals; D005260:Female; D056426:Genetic Loci; D026863:Ixodidae / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic