title: Survey of Cherry necrotic rusty mottle virus and Cherry green ring mottle virus incidence in Korea by Duplex RT-PCR.
abstract: The incidence of Cherry necrotic rusty mottle virus (CNRMV) and Cherry green ring mottle virus (CGRMV) have recently been occurred in Korea, posing a problem for sweet cherry cultivation. Since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. In this study, the incidence of CNRMV and CGRMV in sweet cherry in Gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (RT-PCR) method that can detect both viruses in a single reaction. CNRMV and CGRMV co-infection rates were 29.6%, 53.6%, and 17.6%, respectively, in samples collected from three different sites (Daegu, Gyeongju and Gyeongsan) in Gyeongbuk province during 2012 and 2013. This duplex RT-PCR method offers a simple, rapid, and effective way of identifying CNRMV and CGRMV simultaneously in sweet cherry trees, which can aid in the management of viral infections that could undermine yield.
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea ; College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Mi-Chi
authors.initials: MC
authors.lastname: Yea
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Chang-Gi
authors.initials: CG
authors.lastname: Back
authors.affiliation: College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Kwang-Shik
authors.initials: KS
authors.lastname: Choi
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Kang
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Su-Heon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
keywords: CGRMV; CNRMV; Duplex RT-PCR; Infection rate