title: Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.
abstract: Extracorporeal shockwave treatment was shown to improve orthopaedic diseases and wound healing and to stimulate lymphangiogenesis in vivo. The aim of this study was to investigate in vitro shockwave treatment (IVSWT) effects on lymphatic endothelial cell (LEC) behavior and lymphangiogenesis. We analyzed migration, proliferation, vascular tube forming capability and marker expression changes of LECs after IVSWT compared with HUVECs. Finally, transcriptome- and miRNA analyses were conducted to gain deeper insight into the IVSWT-induced molecular mechanisms in LECs. The results indicate that IVSWT-mediated proliferation changes of LECs are highly energy flux density-dependent and LEC 2D as well as 3D migration was enhanced through IVSWT. IVSWT suppressed HUVEC 3D migration but enhanced vasculogenesis. Furthermore, we identified podoplaninhigh and podoplaninlow cell subpopulations, whose ratios changed upon IVSWT treatment. Transcriptome- and miRNA analyses on these populations showed differences in genes specific for signaling and vascular tissue. Our findings help to understand the cellular and molecular mechanisms underlying shockwave-induced lymphangiogenesis in vivo.
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Rohringer
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holnthoner
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; TAmiRNA GmbH, Muthgasse 11, Vienna, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hackl
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Hoechstaedtplatz 6, Vienna, Austria.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Weihs
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Department of Biochemical Engineering, University of Applied Sciences Technikum Wien, Hoechstaedtplatz 6, Vienna, Austria.
authors.forename: Dominik
authors.initials: D
authors.lastname: Rünzler
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; TAmiRNA GmbH, Muthgasse 11, Vienna, Austria.
authors.forename: Susanna
authors.initials: S
authors.lastname: Skalicky
authors.affiliation: Institute for Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Karbiener
authors.affiliation: Institute for Molecular Biotechnology, Graz University of Technology, Petersgasse 14, Graz, Austria.
authors.forename: Marcel
authors.initials: M
authors.lastname: Scheideler
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Red Cross Blood Transfusion Service, Krankenhausstrasse 7, Linz, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Pröll
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Red Cross Blood Transfusion Service, Krankenhausstrasse 7, Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gabriel
authors.affiliation: Skin and Endothelium Research Division, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Schweighofer
authors.affiliation: Skin and Endothelium Research Division, Department of Dermatology, Medical University of Vienna, Vienna, Austria; Core Facility Imaging, Medical University of Vienna, Vienna, Austria.
authors.forename: Marion
authors.initials: M
authors.lastname: Gröger
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Core Facility Flow Cytometry & Surgical Research Laboratories, Medical University of Vienna, Vienna, Austria.
authors.forename: Andreas
authors.initials: A
authors.lastname: Spittler
authors.affiliation: Austrian Cluster for Tissue Regeneration, Vienna, Austria; Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, Vienna, Austria.
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
authors.affiliation: Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria.
authors.forename: Heinz
authors.initials: H
authors.lastname: Redl
mesh_terms: D002465:Cell Movement / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D042783:Endothelial Cells / Q000473:pathology / Q000528:radiation effects*; D005786:Gene Expression Regulation; D019220:High-Energy Shock Waves; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D042583:Lymphangiogenesis / Q000235:genetics / Q000528:radiation effects*; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology / Q000528:radiation effects*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000528:radiation effects; D059467:Transcriptome / Q000235:genetics; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D035683:MicroRNAs