title: Detection of global DNA hypomethylation of peripheral blood lymphocytes in patients with infantile spasms.
abstract: The pathogenesis of infantile spasms remains unclear. DNA methylation may play a pivotal role in the development of some types of neurological diseases, such as epilepsy. In this study, we aimed to investigate the relationship between global DNA methylation of peripheral blood leukocytes and cryptogenic infantile spasms. DNA from peripheral blood leukocytes was extracted from 20 patients with cryptogenic infantile spasms and 20 gender and age matched healthy controls. Global DNA methylation percentage of peripheral blood leukocytes was measured using a global DNA methylation quantification kit. Global DNA methylation levels of peripheral blood lymphocytes in patients with cryptogenic infantile spasms (23.4 ± 20.0%) were significantly lower than those in healthy controls (46.8 ± 8.4%). Furthermore, we did not find any association between the levels of DNA methylation and effectiveness of Adrenocorticotropic hormone treatment. Our study demonstrates that global DNA hypomethylation of peripheral blood lymphocytes is correlated with infantile spasms. This finding provides information for better understanding of the pathogenesis of infantile spasms.
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiu-Yu
authors.initials: XY
authors.lastname: Shi
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ju
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Department of Pediatrics, Linyi People's Hospital, Linyi 276000, Shandong, China.
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Linyi People's Hospital, Linyi 276000, Shandong, China.
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Chinese PLA General Hospital, Beijing 100853, China; Beijing Institute for Brain Disorder, Beijing 100069, China. Electronic address: zouliping21@hotmail.com.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zou
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000627:therapeutic use; D000927:Anticonvulsants / Q000627:therapeutic use; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D013036:Spasms, Infantile / Q000097:blood* / Q000188:drug therapy / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D000927:Anticonvulsants; D000324:Adrenocorticotropic Hormone
keywords: Infantile spasms; Methylation; Peripheral blood lymphocytes; Stress