title: Characterization and antioxidant activities of the polysaccharides from mycelium of Phellinus pini and culture medium.
abstract: Two polysaccharides isolated and purified from the mycelium (PPM) and its culture medium (PPE) of Phellinus pini using gel filtration were subjected to composition analysis and valuated for the antioxidant activity. The average molecular weights of PPM and PPE were approximately 22.0 and 38.0 kDa, respectively. PPM and PPE were both neutral heteropolysaccharides consisting of mannose, galactose, and glucose with molecular ratios of 2.99:1.00:0.34 and 38.40:1.00:1.76, respectively. In vitro antioxidant assay, PPM and PPE could scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and hydroxyl radical, chelate ferrous ion and reduce ferric ion. The antioxidant activities of PPM were stronger than those of PPE, suggesting that PPM has significant potential as a natural antioxidant agent.
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Agricultural and Animal Husbandry College of Tibet University, Lhasa 860000, Xizang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yuan
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China.
authors.forename: Dongling
authors.initials: D
authors.lastname: Cai
authors.affiliation: Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: jianganji@hotmail.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiao
authors.affiliation: School of Life Sciences, Northeast Normal University, Changchun 130024, China. Electronic address: zhanglp625@163.com.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001487:Basidiomycota / Q000737:chemistry* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry* / Q000378:metabolism; D025282:Mycelium / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003470:Culture Media; D011134:Polysaccharides
keywords: Antioxidant activity; Phellinus pini; Polysaccharides