title: Achieving single-nucleotide resolution of 5-methylcytosine detection with TALEs.
abstract: We report engineered transcription-activator-like effectors (TALEs) as the first DNA-binding molecules that detect 5-methylcytosine (mC) at single-nucleotide resolution with fully programmable sequence selectivity. This is achieved by a design strategy such that a single cytosine (C) in a DNA sequence is selectively interrogated for its mC-modification level by targeting with a discriminatory TALE repeat; other Cs are ignored by targeting with universal-binding TALE repeats.
authors.affiliation: Department of Chemistry, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz (Germany).
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kubik
authors.forename: Daniel
authors.initials: D
authors.lastname: Summerer
mesh_terms: D044503:5-Methylcytosine / Q000032:analysis*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D017931:DNA Primers; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D020449:Repetitive Sequences, Amino Acid; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D015335:Molecular Probes; D011994:Recombinant Proteins; C486715:TXNIP protein, human; D015534:Trans-Activators; D044503:5-Methylcytosine
keywords: DNA methylation; TALE proteins; epigenetics; programmable protein scaffolds; sensors