title: Free fatty acids enhance the oxidative damage induced by ethanol metabolism in an in vitro model.
abstract: In recent years, there has been a growing interest to explore the responsiveness to injury in steatotic hepatocyte. VL-17A cells, which express ADH and Cyp2E1 overloaded with free fatty acids (1 mM of oleic and palmitic acid 2:1) showed an increased oxidative damaged after 24 h free fatty acids treatment when exposed to ethanol (100 mM) for 48 h as a second injury. An increment in reactive oxygen species, determined by DCFH-DA, protein oxidation, and apoptosis were observed although an increase in main antioxidant proteins such as superoxide dismutase 1 and glutathione peroxidase were observed, but failed in gamma-glutamylcysteine synthetase, suggesting a decreased capacity of synthesis of glutathione compared with cells treated only with free fatty acids or ethanol. The increased oxidative stress and toxicity in lipid overloaded VL-17A cells subjected to ethanol exposure were accompanied by increases in Cyp2E1 protein expression. Our data show that lipid loaded in an in vitro model, VL-17A cells, is more susceptible to cell damage and oxidative stress when treated with ethanol.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Ileana
authors.initials: I
authors.lastname: Hernández
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico.
authors.forename: Mayra
authors.initials: M
authors.lastname: Domínguez-Pérez
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de Enferemedades Hepáticas PROMEP-SEP, México, DF, Mexico.
authors.forename: Leticia
authors.initials: L
authors.lastname: Bucio
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de Enferemedades Hepáticas PROMEP-SEP, México, DF, Mexico.
authors.forename: Verónica
authors.initials: V
authors.lastname: Souza
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de Enferemedades Hepáticas PROMEP-SEP, México, DF, Mexico.
authors.forename: Roxana U
authors.initials: RU
authors.lastname: Miranda
authors.affiliation: Nebraska and Western Iowa Veterans Administration Medical Center, University of Nebraska Medical Center, Omaha, NE, United States.
authors.forename: Dahn L
authors.initials: DL
authors.lastname: Clemens
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de Enferemedades Hepáticas PROMEP-SEP, México, DF, Mexico.
authors.forename: Luis Enrique
authors.initials: LE
authors.lastname: Gomez-Quiroz
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana Iztapalapa, México, DF, Mexico; Red Fisiopatología de Enferemedades Hepáticas PROMEP-SEP, México, DF, Mexico. Electronic address: mcgr@xanum.uam.mx.
authors.forename: María Concepción
authors.initials: MC
authors.lastname: Gutiérrez-Ruiz
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000633:toxicity*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005452:Fluoresceins / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005978:Glutathione / Q000096:biosynthesis; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005230:Fatty Acids, Nonesterified; D005452:Fluoresceins; D017382:Reactive Oxygen Species; D014280:Triglycerides; C029569:diacetyldichlorofluorescein; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D019392:Cytochrome P-450 CYP2E1; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione
keywords: Ethanol; Hepatocytes; Oxidative stress; Steatosis; VL-17A