title: Transplantation of bone marrow-derived mesenchymal stem cells facilitates epithelial repair and relieves the impairment of gastrointestinal function in a rat model of enteritis.
abstract: BACKGROUND
  To examine whether the bone marrow-derived MSCs (BM-MSCs) could facilitate epithelial repair and thereby reduce impairment of gastrointestinal structure and function in chronic murine enteritis induced by indomethacin (IDM).
  
  
  METHODS
  MSCs were isolated from young Sprague-Dawley rats. After in vitro expansion and characterization, BM-MSCs were labelled with the fluorescent dye PKH26 and transfused, via the tail veins, into rats with enteritis induced by IDM. The controls were infused with sterile saline. The homing and differentiation of the transplanted BM-MSCs were tracked by means of fluorescent staining. The clinical symptoms of the IDM-treated rats were assessed, and the macroscopic and microscopic histological evaluations of the intestines were performed.
  
  
  RESULTS
  Compared to controls that received saline infusion, BM-MSCs treated rats showed lower scores of weight loss, stool consistency, and stool blood. The PKH26-labelled cells resided at the injured intestine, where they co-localize with the proliferating cell nuclear antigen (PCNA), Lgr-5, and Msi-1. The BM-MSCs treated rats showed significantly higher intestinal villi with larger areas relative to the saline-treated rats.
  
  
  CONCLUSIONS
  The transplanted BM-MSCs are able to recognize the injured intestine, where they proliferate and transdifferentiate into intestinal stem cells which repair the injured intestinal tissues. Therefore, BM-MSCs are able to relieve the impairment of gastrointestinal function in IMD-treated rats.
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qu
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Jia-Zhao
authors.initials: JZ
authors.lastname: Tong
authors.affiliation: Department of Gastroenterology and Hepatology, the Tailai County People's Hospital, Heilongjiang Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Bing-Rong
authors.initials: BR
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology and Hepatology, the Second Affiliated Hospital, Harbin Medical University, Heilongjiang Province, China. Electronic address: shuzhujin@126.com.
authors.forename: Shi-Zhu
authors.initials: SZ
authors.lastname: Jin
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D004195:Disease Models, Animal; D004751:Enteritis / Q000503:physiopathology / Q000601:surgery*; D041981:Gastrointestinal Tract / Q000503:physiopathology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D017207:Rats, Sprague-Dawley