title: The insulinotrophic effect of insulin-like peptide 5 in vitro and in vivo.
abstract: Insulin-like peptide 5 (INSL5), a member of the insulin/relaxin superfamily, can activate the G-protein-coupled receptor relaxin/insulin-like family peptide receptor 4 (RXFP4), but its precise biological functions are largely unknown. Recent studies suggest that INSL5/RXFP4 is involved in the control of food intake and glucose homoeostasis. We report in the present study that RXFP4 is present in the mouse insulinoma cell line MIN6 and INSL5 augments glucose-stimulated insulin secretion (GSIS) both in vitro and in vivo. RXFP4 is also expressed in the mouse intestinal L-cell line GLUTag and INSL5 is capable of potentiating glucose-dependent glucagon-like peptide-1 (GLP-1) secretion in GLUTag cells. We propose that the insulinotrophic effect of INSL5 is probably mediated through stimulation of insulin/GLP-1 secretion and the INSL5/RXFP4 system may be a potential therapeutic target for Type 2 diabetes.
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Dai
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: †Institute of Protein Research, College of Life Sciences and Technology, Tongji University, Shanghai, China.
authors.forename: Zhan-Yun
authors.initials: ZY
authors.lastname: Guo
authors.affiliation: *The National Center for Drug Screening and the Chinese Academy of Sciences Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Shanghai, China.
authors.forename: Ming-Wei
authors.initials: MW
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D057809:HEK293 Cells; D006801:Humans; D007328:Insulin / Q000097:blood; D007340:Insulinoma / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D036361:Peptide Hormones / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; C121388:Insl5 protein, mouse; D007328:Insulin; D036361:Peptide Hormones; D052216:Glucagon-Like Peptide 1