title: Sulfated heterorhamnans from the green seaweed Gayralia oxysperma: partial depolymerization, chemical structure and antitumor activity.
abstract: Sulfated heterorhamnans produced by Gayralia oxysperma were utilized for the preparation of two homogeneous and highly sulfated Smith-degraded products (M(w) of 109 and 251 kDa), which were constituted principally by 3-linked α-L-rhamnosyl units 2- or 4-sulfate and 2-linked α-L-rhamnosyl units 4- or 3,4-sulfate, in different percentages. The homogeneous products and the crude extracts containing the sulfated heterorhamnans showed cytotoxic effect against U87MG cells. These sulfated polysaccharides induced an increase in the number of cells in G1 phase with concomitant increase of the mRNA levels of p53 and p21. The presence of 2-linked disulfated rhamnose residues together with the molecular weight could be important factors to be correlated with the inhibitory effect on human glioblastoma cells.
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Ropellato
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil.
authors.forename: Mariana M
authors.initials: MM
authors.lastname: Carvalho
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil.
authors.forename: Luciana G
authors.initials: LG
authors.lastname: Ferreira
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil. Electronic address: mdn@ufpr.br.
authors.forename: Miguel D
authors.initials: MD
authors.lastname: Noseda
authors.affiliation: Laboratório de Cultivo Celular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Cristiane R
authors.initials: CR
authors.lastname: Zuconelli
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Alan G
authors.initials: AG
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil.
authors.forename: Diogo R B
authors.initials: DRB
authors.lastname: Ducatti
authors.affiliation: Laboratório de Cultivo Celular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Juliana C N
authors.initials: JCN
authors.lastname: Kenski
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil.
authors.forename: Pauline L
authors.initials: PL
authors.lastname: Nasato
authors.affiliation: Laboratório de Cultivo Celular, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil.
authors.forename: Sheila M B
authors.initials: SMB
authors.lastname: Winnischofer
authors.affiliation: Laboratório de Glicobiologia Estrutural de Carboidratos de Algas Marinhas (GLICAM), Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box: 19046, CEP: 81531-980 Curitiba, PR, Brazil. Electronic address: nosedaeu@ufpr.br.
authors.forename: Maria E R
authors.initials: MER
authors.lastname: Duarte
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D000460:Chlorophyta / Q000737:chemistry*; D003837:Deoxy Sugars / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008351:Mannans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015394:Molecular Structure; D058105:Polymerization; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D003837:Deoxy Sugars; D008351:Mannans; D013431:Sulfates; C065836:rhamnan
keywords: Antitumor activity; Chemical structure; Gayralia oxysperma; Green seaweed; Partial depolymerization; Sulfated polysaccharides