title: Isolation of an Adoxophyes orana granulovirus (AdorGV) occlusion body morphology mutant: biological activity, genome sequence and relationship to other isolates of AdorGV.
abstract: A granulovirus (GV) producing occlusion bodies (OBs) with an unusual appearance was isolated from Adoxophyes spp. larvae in the field. Ultrastructural observations revealed that its OBs were significantly larger and cuboidal in shape, rather than the standard ovo-cylindrical shape typical of GVs. N-terminal amino acid sequence analysis of the OB matrix protein from this virus suggested that this new isolate was a variant of Adoxophyes orana granulovirus (AdorGV). Bioassays of this GV (termed AdorGV-M) and an English isolate of AdorGV (termed AdorGV-E) indicated that the two isolates were equally pathogenic against larvae of Adoxophyes honmai. However, AdorGV-M retained more infectivity towards larvae after irradiation with UV light than did AdorGV-E. Sequencing and analysis of the AdorGV-M genome revealed little sequence divergence between this isolate and AdorGV-E. Comparison of selected genes among the two AdorGV isolates and other Japanese AdorGV isolates revealed differences that may account for the unusual OB morphology of AdorGV-M.
authors.affiliation: Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Nakai
authors.affiliation: Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service (USDA-ARS), Beltsville Agricultural Research Center, 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Harrison
authors.affiliation: Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Haruaki
authors.initials: H
authors.lastname: Uchida
authors.affiliation: Yaeyama Branch Office, Okinawa Prefectural Plant Protection Center, 1178-6, Chisokobaru, Hirae, Ishigaki, Okinawa 907-0003, Japan.|Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Ukuda
authors.affiliation: Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Hikihara
authors.affiliation: Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Ishii
authors.affiliation: Tokyo University of Agriculture and Technology, Saiwai, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Kunimi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016679:Genome, Viral; D025722:Granulovirus / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology*; D007814:Larva / Q000821:virology; D007915:Lepidoptera / Q000821:virology; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny