title: Neuroprotective mechanism of BNG-1 against focal cerebral ischemia: a neuroimaging and neurotrophin study.
abstract: BNG-1 is a herb complex used in traditional Chinese medicine to treat stroke. In this study, we attempted to identify the neuroprotective mechanism of BNG-1 by using neuroimaging and neurotrophin analyses of a stroke animal model. Rats were treated with either saline or BNG-1 for 7 d after 60-min middle cerebral artery occlusion by filament model. The temporal change of magnetic resonance (MR) imaging of brain was studied using a 7 Tesla MR imaging (MRI) system and the temporal expressions of neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) in brain were analyzed before operation and at 4 h, 2 d, and 7 d after operation. Compared with the saline group, the BNG-1 group exhibited a smaller infarction volume in the cerebral cortex in T2 image from as early as 4 h to 7 d, less edema in the cortex in diffusion weighted image from 2 to 7 d, earlier reduction of postischemic hyperperfusion in both the cortex and striatum in perfusion image at 4 h, and earlier normalization of the ischemic pattern in the striatum in susceptibility weighted image at 2 d. NT-3 and BDNF levels were higher in the BNG-1 group than the saline group at 7 d. We concluded that the protective effect of BNG-1 against cerebral ischemic injury might act through improving cerebral hemodynamics and recovering neurotrophin generation.
authors.affiliation: Department of Neurology, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan, and Department of Neurology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Nai-Fang
authors.initials: NF
authors.lastname: Chi
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Ho-Ling
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Chiayi Chang Gung Memorial Hospital, Chiayi, Taiwan, and Department of Neurosurgery, Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Jen-Tsung
authors.initials: JT
authors.lastname: Yang
authors.affiliation: Clinical Informatics and Medical Statistics Research Center, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Jr-Rung
authors.initials: JR
authors.lastname: Lin
authors.affiliation: Department of Traditional Chinese Medicine, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan.
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Liao
authors.affiliation: Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan.
authors.forename: Bo-Han
authors.initials: BH
authors.lastname: Peng
authors.affiliation: Department of Traditional Chinese Medicine, En Chu Kong Hospital, New Taipei City, Taiwan.
authors.forename: Yen-Tung
authors.initials: YT
authors.lastname: Lee
authors.affiliation: Stroke Center and Department of Neurology, Chang Gung Memorial Hospital, Linkou Medical Center, Taoyuan, Taiwan, and Chang Gung University College of Medicine, Taoyuan, Taiwan.
authors.forename: Tsong-Hai
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000473:pathology; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008297:Male; D020932:Nerve Growth Factor / Q000032:analysis; D059906:Neuroimaging; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D020933:Neurotrophin 3 / Q000032:analysis; D010726:Phosphodiesterase Inhibitors / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004365:Drugs, Chinese Herbal; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D010726:Phosphodiesterase Inhibitors; D020932:Nerve Growth Factor