title: The complete mitochondrial genome and its remarkable secondary structure for a stonefly Acroneuria hainana Wu (Insecta: Plecoptera, Perlidae).
abstract: The Plecoptera (stoneflies) is a hemimetabolous order of insects, whose larvae are usually used as indicators for fresh water biomonitoring. Herein, we describe the complete mitochondrial (mt) genome of a stonefly species, namely Acroneuria hainana Wu belonging to the family Perlidae. This mt genome contains 13 PCGs, 22 tRNA-coding genes and 2 rRNA-coding genes that are conserved in most insect mt genomes, and it also has the identical gene order with the insect ancestral gene order. However, there are three special initiation codons of ND1, ND5 and COI in PCGs: TTG, GTG and CGA, coding for L, V and R, respectively. Additionally, the 899-bp control region, with 73.30% A+T content, has two long repeated sequences which are found at the 3'-end closing to the tRNA(Ile) gene. Both of them can be folded into a stem-loop structure, whose adjacent upstream and downstream sequences can be also folded into stem-loop structures. It is presumed that the four special structures in series could be associated with the D-loop replication. It might be able to adjust the replication speed of two replicate directions.
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Mingchao
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Xingyue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.
authors.forename: Weihai
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Kang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China.
authors.forename: Xuankun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, China. Electronic address: dyangcau@126.com.
authors.forename: Ding
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D002874:Chromosome Mapping; D004272:DNA, Mitochondrial / Q000737:chemistry*; D017344:Genes, Insect; D049750:Genome, Insect / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D007313:Insecta / Q000235:genetics*; D008928:Mitochondria / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012335:RNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Acroneuria hainana; Mitochondrial genome; Plecoptera