title: Role of MiR-30a in cardiomyocyte autophagy induced by Angiotensin II.
abstract: OBJECTIVE
  The aim of this study was to investigate whether MiR-30a regulates autophagy by regulating the Beclin-1 protein, which is the marker for autophagosomes during myocardial injury, when induced by angiotensin II (Ang II).
  
  
  METHODS
  We randomly assigned 20 rats into two equal groups: Control group and Ang II group. We detected the expression of MiR-30a by quantitative real-time polymerase chain reaction (RT-PCR), and we employed western blotting to detect the protein expression of Beclin-1.
  
  
  RESULTS
  In this study, we found that Ang II induced cardiomyocyte autophagy, together with down-regulation of MiR-30a and upregulation of the Beclin-1 protein. We also found that the Beclin-1 protein is regulated by MiR-30a, by transferring a MiR-30a mimic or AMO-204 into the cardiomyocytes.
  
  
  CONCLUSIONS
  These studies provided evidence that MiR-30a plays an important role in regulating autophagy through the Beclin-1 protein, during myocardial injury induced by Ang II.
authors.affiliation: Department of Cardiology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Jionghua
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Yishan
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Cardiology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Cardiology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China wanglixin3211@163.com.
authors.forename: Ximing
authors.initials: X
authors.lastname: Chen
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1; D015687:Cell Hypoxia; D006331:Heart Diseases / Q000139:chemically induced / Q000473:pathology; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis / Q000187:drug effects*; D032383:Myocytes, Cardiac / Q000187:drug effects*; D051381:Rats
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D000804:Angiotensin II; D007770:L-Lactate Dehydrogenase
keywords: Animal study; Beclin-1; MiR-30a; angiotensin II; autophagosome; autophagy; cardiomyocytes; injury; myocardial injury; protein regulation; rats