title: Effect of extraction methods on property and bioactivity of water-soluble polysaccharides from Amomum villosum.
abstract: In the present study, effect of different extraction methods on property and bioactivity of water-soluble polysaccharides (WSP) from the seeds of Amomum villosum were investigated. Firstly, four different extraction methods were used to extract WSP, which include hot water extraction (HWE), ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and enzyme-assisted extraction (EAE). As a result, four WSP samples, WSP(H), WSP(U), WSP(M) and WSP(E) were acquired. Then, the difference of four WSP samples in yield, characterization and antioxidant activities in vitro were further compared. Experimental results showed that the four WSP samples had the same monosaccharide composition, but mere difference in the content; they all had typical IR spectra characteristic of polysaccharides. WSP(U) contained the highest contents of uronic acid and sulfate. The yield of WSP(U) was the highest and its antioxidant activity was the best. These results suggested that ultrasonic-assisted extraction was the best extraction method for WSP.
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Affiliated Huaian Hospital of Xuzhou Medical College, Huaian 223002, Jiangsu, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China.
authors.forename: Mianjie
authors.initials: M
authors.lastname: Wan
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Li
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China.
authors.forename: Man
authors.initials: M
authors.lastname: Cao
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510006 Guangdong, PR China. Electronic address: danyan64@21cn.com.
authors.forename: Danyan
authors.initials: D
authors.lastname: Zhang
mesh_terms: D035801:Amomum / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods*; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012995:Solubility; D013481:Superoxides / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D014867:Water; D013481:Superoxides; D007501:Iron
keywords: Amomum villosum; Antioxidant activity; Extraction methods; Property; Water-soluble polysaccharides