title: Relationship between the expression of phosphorylated heat shock protein beta-1 with lymph node metastases of breast cancer.
abstract: BACKGROUND
  Heat shock protein beta-1 (HspB1) is a chaperone of the sHsp (small heat shock protein). The common functions of sHsps are chaperone activity, inhibition of apoptosis, regulation of cell development, and cell differentiation, take part in signal transduction.
  
  
  OBJECTIVE
  To study the intracellular localization of phosphorylated features and non-phosphorylated forms of HspB1 in primary breast cancer cells and to evaluate their relationship with regional lymphatic metastasis.
  
  
  METHODS
  Tumor biopsies of breast tissue were collected from 100 patients with a confirmed diagnosis of invasive carcinoma, nonspecific type, between the ages of 31-80 years. Immunohistochemistry was used to determine the intracellular localization of phosphorylated and non-phosphorylated forms of HspB1.
  
  
  RESULTS
  The result of this study showed that biopsies from patients with lymph node metastasis exhibited significantly higher levels of phosphorylated forms of HspB1 in the nucleus and cytoplasm compared with the group without lymph node metastasis. Analysis showed that the expression of phosphorylated forms of the chaperone HspB1 correlates with the amount and percentage of lymph node metastases affected.
  
  
  CONCLUSIONS
  The nuclear expression of phosphorylated and non-phosphorylated forms of the chaperone HspB1 is a marker of tumor cells associated with lymphatic metastasis of breast cancer.
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation Laboratory for Translational Cellular and Molecular Biomedicine, Tomsk State University, Tomsk, Russian Federation Siberian State Medical University, Tomsk, Russian Federation.
authors.forename: Evgeniya V
authors.initials: EV
authors.lastname: Kaigorodova
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation Laboratory for Translational Cellular and Molecular Biomedicine, Tomsk State University, Tomsk, Russian Federation Siberian State Medical University, Tomsk, Russian Federation.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Zavyalova
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Bogatyuk
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation.
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Tarabanovskaya
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation Siberian State Medical University, Tomsk, Russian Federation.
authors.forename: Elena M
authors.initials: EM
authors.lastname: Slonimskaya
authors.affiliation: Tomsk Cancer Research Institute, Tomsk, Russian Federation Siberian State Medical University, Tomsk, Russian Federation.
authors.forename: Vladimir M
authors.initials: VM
authors.lastname: Perelmuter
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D055551:HSP27 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008875:Middle Aged; D018832:Molecular Chaperones; D009367:Neoplasm Staging; D010766:Phosphorylation; D011446:Prospective Studies
chemical_list: D014408:Biomarkers, Tumor; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones
keywords: Breast cancer; heat shock protein beta-1 (HspB1); immunohistochemistry; lymph node metastases; prognostic markers