title: Vectors expressing chimeric Japanese encephalitis dengue 2 viruses.
abstract: Vectors based on self-replicating RNAs (replicons) of flaviviruses are becoming powerful tool for expression of heterologous genes in mammalian cells and development of novel antiviral and anticancer vaccines. We constructed two vectors expressing chimeric viruses consisting of attenuated SA14-14-2 strain of Japanese encephalitis virus (JEV) in which the PrM/M-E genes were replaced fully or partially with those of dengue 2 virus (DENV-2). These vectors, named pJED2 and pJED2-1770 were transfected to BHK-21 cells and produced chimeric viruses JED2V and JED2-1770V, respectively. The chimeric viruses could be passaged in C6/36 but not BHK-21 cells. The chimeric viruses produced in C6/36 cells CPE 4-5 days after infection and RT-PCR, sequencing, immunofluorescence assay (IFA) and Western blot analysis confirmed the chimeric nature of produced viruses. The immunogenicity of chimeric viruses in mice was proved by detecting DENV-2 E protein-specific serum IgG antibodies with neutralization titer of 10. Successful preparation of infectious clones of chimeric JEV-DENV-2 viruses showed that JEV-based expression vectors are fully functional.
authors.forename: Y
authors.initials: Y
authors.lastname: Wei
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D002678:Chimera / Q000235:genetics / Q000276:immunology / Q000502:physiology; D003715:Dengue / Q000276:immunology / Q000821:virology*; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D004664:Encephalitis Virus, Japanese / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D004672:Encephalitis, Japanese / Q000276:immunology / Q000821:virology*; D005818:Genetic Engineering; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice
chemical_list: D000914:Antibodies, Viral