title: Controlled release by novel lysostaphin-loaded hydroxyapatite/chitosan composites.
abstract: Lysostaphin is highly effective on eliminating methicillin resistant Staphylococcus aureus (MRSA). In order to achieve controlled release of lysostaphin, a biocompatible drug carrier is needed. Hydroxyapatite/chitosan (HA/CS) composites were chosen to carry lysostaphin and sample composites with different weight ratios of HA to CS, including 80/20, 70/30, 60/40, and 40/60, were prepared. Multiple analyses were performed to determine the structural and physicochemical properties of the composites, including scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. We immersed HA/CS composites loaded with 1 wt% lysostaphin to test in vitro release activity and cultured MC3T3-E1 cells to carry out biocompatibility test. The result of the release behavior of the composites revealed that the controlled release of lysostaphin from 60/40 HA/CS composites was the highest release rate of (87.4 ± 2.8)%, which lasted for 120 hours. In biocompatibility testing, MC3T3-E1 cells were able to proliferate on the surface of these composites, and the extract liquid from the composites could increase the growth of the cells. These results demonstrate the controlled release of lysostaphin from HA/CS composites and their biocompatibility, suggesting the potential application of these composites to bone injury and infection applications.
authors.forename: Jin-Cheng
authors.initials: JC
authors.lastname: Wang
authors.forename: Bai
authors.initials: B
authors.lastname: Xue
authors.forename: Kui-Kui
authors.initials: KK
authors.lastname: Ge
authors.forename: Yi-Han
authors.initials: YH
authors.lastname: Wang
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Li
authors.forename: Qing-Shan
authors.initials: QS
authors.lastname: Huang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008248:Lysostaphin / Q000494:pharmacology*; D008422:Materials Testing; D055624:Methicillin-Resistant Staphylococcus aureus; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D048271:Chitosan; D017886:Durapatite; D008248:Lysostaphin