title: Gold nanoparticles in the engineering of antibacterial and anticoagulant surfaces.
abstract: Simultaneous antibacterial and anticoagulant surfaces have been prepared by immobilization of engineered gold nanoparticles onto different kinds of surfaces. The gold nanoparticle core is surrounded by a hemocompatible, anticoagulant polysaccharide, 6-O chitosan sulfate, which serves as reduction and stabilizing agent for the generation of gold nanoparticles in a microwave mediated reaction. The particle suspension shows anticoagulant activity, which is investigated by aPTT and PT testing on citrated blood samples of three patients suffering from congenital or acquired bleeding disorders. The amount of nanoparticles deposited on the surfaces is quantified by a quartz crystal microbalance with dissipation unit. All gold containing surfaces exhibit excellent antimicrobial properties against the chosen model organism, Escherichia coli MG 1655 [R1-16]. Moreover, blood plasma coagulation times of the surfaces are increased after deposition of the engineered nanoparticles as demonstrated by QCM-D.
authors.affiliation: Institute for Chemistry, University of Graz, Graz, Austria; Institute for Chemistry and Technology of Materials, Graz University of Technology, Graz, Austria.
authors.forename: Heike M A
authors.initials: HMA
authors.lastname: Ehmann
authors.affiliation: Institute for Chemistry, University of Graz, Graz, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Breitwieser
authors.affiliation: Institute for Chemistry and Technology of Materials, Graz University of Technology, Graz, Austria.
authors.forename: Sascha
authors.initials: S
authors.lastname: Winter
authors.affiliation: Institute for Electron Microscopy and Fine Structure Research, Centre for Electron Microscopy Graz, Graz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gspan
authors.affiliation: Institute for Chemistry, University of Graz, Graz, Austria.
authors.forename: Günther
authors.initials: G
authors.lastname: Koraimann
authors.affiliation: Faculty of Medicine, University of Maribor, Maribor, Slovenia.
authors.forename: Uros
authors.initials: U
authors.lastname: Maver
authors.affiliation: Center of Transfusion Medicine, University Medical Centre Maribor, 2000 Maribor, Slovenia.
authors.forename: Marija
authors.initials: M
authors.lastname: Sega
authors.affiliation: Joanneum Research Materials, Institute for Surface Technologies, 8160 Weiz, Austria.
authors.forename: Stefan
authors.initials: S
authors.lastname: Köstler
authors.affiliation: Institute for the Engineering and Design of Materials, University of Maribor, Maribor, Slovenia.
authors.forename: Karin
authors.initials: K
authors.lastname: Stana-Kleinschek
authors.affiliation: Institute for Chemistry and Technology of Materials, Graz University of Technology, Graz, Austria; Institute for the Engineering and Design of Materials, University of Maribor, Maribor, Slovenia. Electronic address: stefan.spirk@tugraz.at.
authors.forename: Stefan
authors.initials: S
authors.lastname: Spirk
authors.affiliation: Institute for Chemistry, University of Graz, Graz, Austria.
authors.forename: Volker
authors.initials: V
authors.lastname: Ribitsch
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D000925:Anticoagulants / Q000737:chemistry* / Q000494:pharmacology*; D002214:Capsules; D002482:Cellulose / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D004738:Engineering; D004926:Escherichia coli / Q000187:drug effects; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D053768:Metal Nanoparticles; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D013431:Sulfates / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D000925:Anticoagulants; D002214:Capsules; D013431:Sulfates; D006046:Gold; D002482:Cellulose; D048271:Chitosan
keywords: Anticoagulant activity; Antimicrobial surfaces; Chitosan sulfate; Gold nanoparticles; Quartz crystal microbalance