title: Effect of BMPRIB gene silencing by siRNA on apoptosis and steroidogenesis of porcine granulosa cells.
abstract: Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs). The objective of this study was to determine the effects of BMPRIB gene silencing, by small interfering RNA (siRNA), on the apoptosis and steroidogenesis of porcine GCs, and the expression of cell cycle-related and apoptosis-related genes. Results indicate that the BMPRIB siRNA caused specific inhibition of BMPRIB mRNA expression after transfection. Knockdown of the BMPRIB gene significantly inhibited porcine GCs proliferation and estradiol production, while inducing apoptosis of porcine GCs. Additionally, the declined expression of the BMPRIB gene changed the expressions of CylinD2, Cdk2, Bcl-2, and Cyp19a1. These findings provide an important role of BMPRIB in the regulation of apoptosis and steroidogenesis of porcine GCs.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Li
authors.affiliation: Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin, China.
authors.forename: W
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: P
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China xuyinxue@njau.edu.cn.
authors.forename: Y X
authors.initials: YX
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D049628:Body Size; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics* / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D020868:Gene Silencing; D006107:Granulosa Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D051901:Smad4 Protein / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000096:biosynthesis*; D034421:Sus scrofa; D014162:Transfection
chemical_list: D018797:Cell Cycle Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051901:Smad4 Protein; D013256:Steroids; D052005:Bone Morphogenetic Protein Receptors, Type I