title: Pretransplant urinary proteome analysis does not predict development of chronic kidney disease after liver transplantation.
abstract: OBJECTIVE
  Chronic kidney disease (CKD) is a common complication after liver transplantation. Kidney biopsies cannot be easily performed before liver transplantation to predict patients at high risk for CKD. The aim of our study was to determine whether pre-, peri- and post-transplant factors, as well as peptides present in preliver transplant urine samples were associated with loss in kidney function at 6 months post-transplantation using proteome analysis.
  
  
  METHODS
  Eighty patients who underwent a liver transplantation and that had pretransplant glomerular filtration rate (GFR) value of ≥60 mL/min/1.73 m² (MDRD) were included in the study.
  
  
  RESULTS
  GFR decreased significantly after transplantation. At month 6 post-transplantation, 40 patients displayed a CKD, i.e. eGFR of <60 mL/min/1.73 m², while the other 40 patients did not. Although thousands of peptides were identified, none was significantly associated with the development of CKD at 6 months after liver transplantation. Moreover, using a urinary peptidome classifier to detect preexisting CKD, no difference was found in CKD scores between the 2 groups. After analysis of a large number of pre-, peri- and post-transplant parameters, viral hepatitis as a cause for liver transplantation was the sole independent predictive factor for CKD. No difference in peptides with differential urinary abundance between patients who received a graft for virus related liver disease vs. all other causes of liver disease was observed.
  
  
  CONCLUSIONS
  Urinary peptidome analysis before liver transplantation failed to identify a peptide pattern associated with the development of CKD at 6 months after liver transplantation.
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
authors.forename: David
authors.initials: D
authors.lastname: Milongo
authors.affiliation: U1048, Institut of Cardiovascular and Metabolic Disease, Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France.|Université Toulouse III Paul-Sabatier, Toulouse, France.
authors.forename: Jean-Loup
authors.initials: JL
authors.lastname: Bascands
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Huart
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
authors.forename: Laure
authors.initials: L
authors.lastname: Esposito
authors.affiliation: U1048, Institut of Cardiovascular and Metabolic Disease, Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France.|Institut of Cardiovascular and Metabolic Disease, Plateau de Protéomique des Liquides Biologiques, Toulouse, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Breuil
authors.affiliation: HybridStat Predictive Analytics OE, Athens, Greece.
authors.forename: Panagiotis
authors.initials: P
authors.lastname: Moulos
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Germany.|Charite-Universitatsmedizin Berlin, Berlin, Germany.
authors.forename: Justyna
authors.initials: J
authors.lastname: Siwy
authors.affiliation: Mosaiques Diagnostics GmbH, Hannover, Germany.
authors.forename: Adela
authors.initials: A
authors.lastname: Ramírez-Torres
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
authors.forename: David
authors.initials: D
authors.lastname: Ribes
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Lavayssière
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.|Université Toulouse III Paul-Sabatier, Toulouse, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Del Bello
authors.affiliation: Université Toulouse III Paul-Sabatier, Toulouse, France.|Department of Surgery and Liver Transplantation, Toulouse, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Muscari
authors.affiliation: Internal Medecine-Digestive Department, CHU Purpan, Toulouse, France.|UMR 152, IRD, Université Toulouse III Paul-Sabatier, Toulouse, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Alric
authors.affiliation: Université Toulouse III Paul-Sabatier, Toulouse, France.|Department of Hepatology, Federation Digestive, CHU Purpan, Toulouse, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Bureau
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.|Université Toulouse III Paul-Sabatier, Toulouse, France.|IFR-BMT, INSERM U1043, CHU Purpan, Toulouse, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Rostaing
authors.affiliation: U1048, Institut of Cardiovascular and Metabolic Disease, Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France.|Université Toulouse III Paul-Sabatier, Toulouse, France.
authors.forename: Joost P
authors.initials: JP
authors.lastname: Schanstra
authors.affiliation: Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.|Université Toulouse III Paul-Sabatier, Toulouse, France.|IFR-BMT, INSERM U1043, CHU Purpan, Toulouse, France.
authors.forename: Nassim
authors.initials: N
authors.lastname: Kamar
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000652:urine; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D008107:Liver Diseases / Q000150:complications / Q000175:diagnosis / Q000601:surgery*; D016031:Liver Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011446:Prospective Studies; D011507:Proteinuria / Q000150:complications / Q000175:diagnosis* / Q000652:urine; D040901:Proteomics / Q000379:methods*; D051436:Renal Insufficiency, Chronic / Q000175:diagnosis / Q000209:etiology* / Q000503:physiopathology; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D016482:Urinalysis
chemical_list: D015415:Biomarkers
keywords: chronic kidney disease; liver transplantation; proteome analysis; urine; viral hepatitis