title: Cell cycle control by natural phenols in cisplatin-resistant cell lines.
abstract: Fifteen plant polyphenols, including flavonoids, cinnamic acids, coumarins and capsaicin, were investigated for their capacity to suppress cell growth and regulate the cell cycle of in vitro human ovarian carcinoma (2008 cell line) and cervix squamous carcinoma cells (A431), and their cisplatin (CDDP)-resistant subclones (C13 and A431Pt, respectively). Evaluation of the cytotoxic effects of the polyphenols (0.01-100 μM) indicated that especially rhein and quercetin were almost equiactive in wild type and CDDP-resistant cells, indicating lack of cross-resistance with cisplatin. Capsaicin was more potent in CDDP-resistant subclones than in wild type cells. The order of their potencies is flavonoids > anthraquinones > vanilloids > coumarins > phenols, cinnamic acids. The natural phenols which were most cytotoxic (rhein, quercetin and capsaicin) were able to cause the arrest of the cancer cell cycle, suggesting that specific cell cycle regulatory proteins are possibly involved in their intracellular mechanism of action. In particular, the natural compounds were revealed to be more active in CDDP-resistant cells than in wild types, especially inducing apoptotic death.
authors.forename: Daniela
authors.initials: D
authors.lastname: Catanzaro
authors.forename: Caterina
authors.initials: C
authors.lastname: Vianello
authors.forename: Eugenio
authors.initials: E
authors.lastname: Ragazzi
authors.forename: Laura
authors.initials: L
authors.lastname: Caparrotta
authors.forename: Monica
authors.initials: M
authors.lastname: Montopoli
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002211:Capsaicin / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D002934:Cinnamates / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology*; D003374:Coumarins / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D005260:Female; D006801:Humans; D010636:Phenols / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D002934:Cinnamates; D003374:Coumarins; D010636:Phenols; C029010:cinnamic acid; D002945:Cisplatin; D002211:Capsaicin