title: Pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (Laodelphax striatellus).
abstract: Yeast-like symbionts (YLS) are endosymbionts that promote the growth of delphacid planthoppers (Hemiptera: Delphacidae), some of which are pests on cultivated rice. Identification and characterization of YLS growth can be helpful for pest control, because it has been demonstrated that there is a variety of YLS in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. So, elucidation of the species of YLS in planthopper is crucial for exploiting a new way to control planthopper. In this study, a new isolated of YLS was obtained from the small brown planthopper, Laodelphax striatellus, which was cultured in vitro, simultaneously identified as Pichia anomala based on its phylogenetic analysis. In order to confirm the existence of P. anomala in the L. striatellus body, we used the denaturing gradient gel electrophoresis (DGGE) to identify the YLS and obtain the specific bands for P. anomala. The quantification and localization of P. anomala in L. striatellus samples were determined by fluorescent in situ hybridization (FISH) using genus-specific 18S rDNA targeted probe. The result confirmed that a certain number of P. anomala exist in L. striatellus's abdomen. Subsequently, the variation and copy number of P. anomala in different L. striatellus instars was measured by using absolute quantitative real-time PCR (qPCR), the results indicated that the new isolated strain was closely related to the developmental process of L. striatellus.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ma
authors.affiliation: Department of Plant and Soil Sciences, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Yolanda H
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China. Electronic address: yuxiaoping19630306@163.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Yu
mesh_terms: D000818:Animals; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D005260:Female; D014644:Genetic Variation / Q000235:genetics; D006430:Hemiptera / Q000033:anatomy & histology / Q000254:growth & development / Q000382:microbiology*; D017404:In Situ Hybridization, Fluorescence; D009758:Nymph / Q000382:microbiology; D012275:Oryza / Q000469:parasitology; D010802:Phylogeny; D010843:Pichia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013559:Symbiosis; D014774:Virulence
chemical_list: D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S
keywords: Absolute quantitative real-time PCR; Culture in vitro; Instar nymph; Laodelphax striatellus; Pichia anomala; Yeast-like symbionts