title: In vitro assessment of the effect of vaccine-targeted human papillomavirus (HPV) depletion on detection of non-vaccine HPV types: implications for post-vaccine surveillance studies.
abstract: In populations where the prevalence of vaccine-targeted HPV types has been reduced significantly due to widespread vaccination of the target population, the sensitivity of some consensus PCR-based assays to detect remaining HPV types may be altered, leading to misrepresentations of prevalence. Importantly, this may lead to false indications of type replacement in vaccinated populations. To assess whether excess vaccine-targeted HPV DNA resulted in reduced detection of other genotypes on the Roche HPV linear array genotype assay, simulated samples containing 1000 copies of one or two high-risk HPV DNA genomes in the presence and the absence of 10,000 copies of the HPV16 genome were tested. HPV16 alone did not affect detection of other high-risk genotypes; however when HPV16 and an additional genotype were present, detection of HPV31, 33, 51 or 59 was impeded, indicating potential for misrepresentation of population-based prevalence of these genotypes and false evidence for type replacement following vaccination.
authors.affiliation: Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia. Electronic address: alyssa.cornall@mcri.edu.au.
authors.forename: Alyssa M
authors.initials: AM
authors.lastname: Cornall
authors.affiliation: Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia.
authors.forename: Samuel
authors.initials: S
authors.lastname: Phillips
authors.affiliation: Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia.
authors.forename: Eleanor
authors.initials: E
authors.lastname: Cummins
authors.affiliation: Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville 3052, Australia.
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Garland
authors.affiliation: Regional HPV Labnet Reference Laboratory, The Royal Women's Hospital, Parkville 3052, Australia; Infectious Diseases & Microbiology Research Group, Murdoch Childrens Research Institute, Parkville 3052, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Parkville 3052, Australia.
authors.forename: Sepehr N
authors.initials: SN
authors.lastname: Tabrizi
mesh_terms: D060085:Coinfection / Q000175:diagnosis*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005188:False Negative Reactions; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral
keywords: False negatives; Genotyping; HPV; Surveillance; Type replacement; Vaccine