title: Anti-mycobacterial nucleoside antibiotics from a marine-derived Streptomyces sp. TPU1236A.
abstract: Five new nucleoside antibiotics, named streptcytosines A-E (1-5), and six known compounds, de-amosaminyl-cytosamine (6), plicacetin (7), bamicetin (8), amicetin (9), collismycin B (10), and SF2738 C (11), were isolated from a culture broth of Streptomyces sp. TPU1236A collected in Okinawa, Japan. The structures of new compounds were elucidated on the basis of their spectroscopic data (HRFABMS, IR, UV, and 2D NMR experiments including 1H-1H COSY, HMQC, HMBC, and NOESY spectra). Streptcytosine A (1) belonged to the amicetin group antibiotics, and streptcytosines B-E (2-5) were derivatives of de-amosaminyl-cytosamine (6), 2,3,6-trideoxyglucopyranosyl cytosine. Compound 1 inhibited the growth of Mycobacterium smegmatis (MIC = 32 µg/mL), while compounds 2-5 were not active at 50 µg/disc. Bamicetin (8) and amicetin (9) showed the MICs of 16 and 8 µg/mL, respectively.
authors.affiliation: Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan. 21252502@is.tohoku-pharm.ac.jp.
authors.forename: Ying-Yue
authors.initials: YY
authors.lastname: Bu
authors.affiliation: Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan. yamazaki@tohoku-pharm.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamazaki
authors.affiliation: Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan. ukai_k@tohoku-pharm.ac.jp.
authors.forename: Kazuyo
authors.initials: K
authors.lastname: Ukai
authors.affiliation: Faculty of Pharmaceutical Sciences, Tohoku Pharmaceutical University, Aoba-ku, Sendai 981-8558, Japan. mnami@tohoku-pharm.ac.jp.
authors.forename: Michio
authors.initials: M
authors.lastname: Namikoshi
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D007564:Japan; D008386:Marine Biology; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects; D009705:Nucleosides / Q000737:chemistry* / Q000494:pharmacology*; D011725:Pyridines / Q000737:chemistry / Q000494:pharmacology; D011741:Pyrimidine Nucleosides / Q000737:chemistry / Q000494:pharmacology; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D009705:Nucleosides; D011725:Pyridines; D011741:Pyrimidine Nucleosides; C091809:SF 2738C; C004413:amicetin; C089908:collismycin B; D015082:2,2'-Dipyridyl