title: Development and Standardization of Dot - ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT).
abstract: BACKGROUND
  Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world.
  
  
  METHODS
  Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect ELISA and direct agglutination test (DAT) were also evaluated by dot-ELISA afterwards.
  
  
  RESULTS
  Kappa statistical analysis revealed that Dot-ELISA has good agreements with ELISA as well as the DAT and also, Mc Nemar's analyzing showed that this procedure has acceptable ability to discriminate positive results. Relative sensitivity and specificity of Dot-ELISA were respectively 92.63% and 89.16% and 93.4% and 90.8% in comparison with ELISA and DAT.
  
  
  CONCLUSIONS
  Since the dot-ELISA is easy, inexpensive and not needed high experience to interpret the results, it is superior to ELISA and DAT when we aim to screen the cattle on the farm and slaughterhouses or when the laboratory equipment is not available.
authors.affiliation: Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Hamidinejat
authors.affiliation: Department of Clinical Sciences, Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Mohamad Rahim
authors.initials: MR
authors.lastname: Haji Hajikolaei
authors.affiliation: Department of Pathobiology, Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Ghorbanpoor
authors.affiliation: Razi Institute, Shiraz Branch, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Namavari
authors.affiliation: Veterinary Faculty, Shahid Chamran University, Ahvaz, Iran.
authors.forename: Sara Mohamad Ali
authors.initials: SM
authors.lastname: Gol
keywords: Dot – ELISA; Iran; Neospora caninum; Sensetivity; Serodiagnosis; Specificity