title: Injectable, interconnected, high-porosity macroporous biocompatible gelatin scaffolds made by surfactant-free emulsion templating.
abstract: High-porosity interconnected, thermoresponsive macroporous hydrogels are prepared from oil-in-water high internal phase emulsions (HIPEs) stabilized by gelatin-graft-poly(N-isopropylacrylamide). PolyHIPEs are obtained by gelling HIPEs utilizing the thermoresponsiveness of the copolymer components. PolyHIPEs properties can be controlled by varying the aqueous phase composition, internal phase volume ratio, and gelation temperature. PolyHIPEs respond to temperature changes experienced during cell seeding, allowing fibroblasts to spread, proliferate, and penetrate into the scaffold. Encapsulated cells survive ejection of cell-laden hydrogels through a hypodermic needle. This system provides a new strategy for the fabrication of safe injectable biocompatible tissue engineering scaffolds.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore; Polymer and Composite Engineering (PaCE) Group, Department of Chemical Engineering, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.
authors.forename: Bernice H L
authors.initials: BH
authors.lastname: Oh
authors.forename: Alexander
authors.initials: A
authors.lastname: Bismarck
authors.forename: Mary B
authors.initials: MB
authors.lastname: Chan-Park
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D004655:Emulsions / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D016062:Porosity; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D004655:Emulsions; D020100:Hydrogels; D013501:Surface-Active Agents; C052970:poly-N-isopropylacrylamide; D005780:Gelatin
keywords: cell encapsulation; gelatin; high internal phase emulsions; hydrogels; self-emulsifying; thermoresponsive