title: Phospholipid bilayer nanodiscs: a powerful tool to study the structural organization and biochemical reactivity of proteins in membrane-like environments.
abstract: Nanodiscs are disc-like structures formed by two copies of a membrane scaffold protein, engineered from apolipoprotein A-I, surrounding a phospholipid mixture that can incorporate membrane proteins preserving their natural properties. They behave as soluble entities allowing the use of high-resolution structural techniques to determine the structural organization of the embedded membrane protein, and the use of solution biochemical-biophysical tools to measure its activity, assembly and interactions with other proteins in membranelike environments. In addition, nanodiscs are biocompatible which makes them an attractive technology to be used in therapy, drug discovery, and other biotechnological applications.
authors.forename: Víctor M
authors.initials: VM
authors.lastname: Hernández-Rocamora
authors.forename: Concepción
authors.initials: C
authors.lastname: García-Montañés
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), 28040, Madrid, Spain. Victor.Hernandez-Rocamora@newcastle.ac.uk.
authors.forename: Germán
authors.initials: G
authors.lastname: Rivas
mesh_terms: D016632:Apolipoprotein A-I / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D018797:Cell Cycle Proteins / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D003598:Cytoskeletal Proteins / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry; D006801:Humans; D008051:Lipid Bilayers / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008565:Membrane Proteins / Q000737:chemistry*; D008854:Microscopy, Electron; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010743:Phospholipids / Q000737:chemistry*; D011485:Protein Binding; D015202:Protein Engineering; D054730:Protein Interaction Domains and Motifs
chemical_list: D016632:Apolipoprotein A-I; D001426:Bacterial Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029968:Escherichia coli Proteins; C071525:FtsZ protein, Bacteria; D008051:Lipid Bilayers; D008565:Membrane Proteins; D010743:Phospholipids; C103795:ZipA protein, E coli