title: Inhibitory/suppressive oligodeoxynucleotide nanocapsules as simple oral delivery devices for preventing atopic dermatitis in mice.
abstract: Here, we report a simple and low-cost oral oligodeoxynucleotide (ODN) delivery system targeted to the gut Peyer's patches (PPs). This system requires only Dulbecco's modified eagle's medium, calcium chloride, ODNs, and basic laboratory equipment. ODN nanocapsules (ODNcaps) were directly delivered to the PPs through oral administration and were taken up by macrophages in the PPs, where they induced an immune response. Long-term continuous oral dosing with inhibitory/suppressive ODNcaps (iODNcaps, "iSG3caps" in this study) was evaluated using an atopic dermatitis mouse model to visually monitor disease course. Administration of iSG3caps improved skin lesions and decreased epidermal thickness. Underlying this effect is the ability of iSG3 to bind to and prevent phosphorylation of signal transducer and activator of transcription 6, thereby blocking the interleukin-4 signaling cascade mediated by binding of allergens to type 2 helper T cells. The results of our iSG3cap oral delivery experiments suggest that iSG3 may be useful for treating allergic diseases.
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University, Kamiina, Japan.
authors.forename: Yeqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Graduate School of Agriculture, Shinshu University, Kamiina, Japan.
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: 1] Interdisciplinary Graduate School of Science and Technology, Shinshu University, Kamiina, Japan [2] Research Fellow of the Japan Society for the Promotion of Science (JSPS), Chiyoda-ku, Japan.
authors.forename: Suguru
authors.initials: S
authors.lastname: Shigemori
authors.affiliation: Graduate School of Agriculture, Shinshu University, Kamiina, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Graduate School of Agriculture, Shinshu University, Kamiina, Japan.
authors.forename: Kazushi
authors.initials: K
authors.lastname: Oshiro
authors.affiliation: Graduate School of Agriculture, Shinshu University, Kamiina, Japan.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Graduate School of Agriculture, Shinshu University, Kamiina, Japan.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine and Clinical Immunology, Graduate School of Medicine, Yokohama City University, Yokohama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Kamiina, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Yonekura
authors.affiliation: Frontier Agriscience and Technology Center (FAST), Shinshu University, Kamiina, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Tanaka
authors.affiliation: Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Haruki
authors.initials: H
authors.lastname: Kitazawa
authors.affiliation: 1] Interdisciplinary Graduate School of Science and Technology, Shinshu University, Kamiina, Japan [2] Graduate School of Agriculture, Shinshu University, Kamiina, Japan [3] Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research (ICCER), Shinshu University, Kamiina, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shimosato
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003876:Dermatitis, Atopic / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control / Q000628:therapy; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D000067596:Interleukin-33 / Q000096:biosynthesis; D008262:Macrophage Activation / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D053769:Nanocapsules / Q000648:ultrastructure; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage* / Q000276:immunology*; D010581:Peyer's Patches / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D000067596:Interleukin-33; D053769:Nanocapsules; D009838:Oligodeoxyribonucleotides