title: PCNA and grade in 13 canine oral squamous cell carcinomas: association with prognosis.
abstract: This study evaluated the prognosis factors of age, tumour size, anatomic location, histological grade and proliferating cell nuclear antigen (PCNA) expression in 13 dogs with oral squamous cell carcinoma (OSCC) with bone invasion and without signs of lymph node or distant metastasis. All animals were treated with radical excision performed with at least 1 cm margin, based on computed tomography images. In the 2-year follow-up, median disease-free survival was 138 days for dogs with grade 3 tumours and was not reached for those with grade 2 tumours. Grade 3 tumours and PCNA labelling index ≥65% were related with a shorter disease-free survival time and consequently poor prognosis (p = 0.003 and p = 0.034, respectively). Mean PCNA labelling index was significantly higher in recurrent cases (p = 0.011). Histological grade and PCNA expression may be important prognosis factors in canine OSCC.
authors.affiliation: CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal.
authors.forename: L A
authors.identifier: http://orcid.org/0000-0002-2424-2106
authors.initials: LA
authors.lastname: Mestrinho
authors.affiliation: CBIOS, Universidade Lusófona de Humanidade e Tecnologias, Campo Grande, 376, 1749-024, Lisboa, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Faísca
authors.affiliation: CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal.
authors.forename: M C
authors.initials: MC
authors.lastname: Peleteiro
authors.affiliation: CIISA, Faculdade de Medicina Veterinária, ULisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal.
authors.forename: M M R E
authors.initials: MM
authors.lastname: Niza
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D018572:Disease-Free Survival; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004285:Dogs; D005260:Female; D053208:Kaplan-Meier Estimate; D008297:Male; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D009367:Neoplasm Staging; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D018809:Proliferating Cell Nuclear Antigen
keywords: comparative oncology; oncology; small animal; surgical oncology; tumour biology