title: Expression of HMGB1 in the periodontal tissue subjected to orthodontic force application by Waldo's method in mice.
abstract: Recent studies indicate that high mobility group box protein 1 (HMGB1) originating from periodontal ligament (PDL) cells can be a potential regulator in the process of orthodontic tooth movement and periodontal tissue remodeling. The aim of this study is to investigate HMGB1 expression in periodontal tissue during orthodontic tooth movement in mice according to Waldo's method. Six 7-week-old C57BL6 mice were used in these experiments. The elastic band was inserted into the teeth space between the right first and second maxillary molars. After 3 days of mechanical loading, mice were fixed with transcardial perfusion of 4 % paraformaldehyde in 0.1 M phosphate buffer (pH 7.4), and the maxillary was extracted for histochemical analyses. The histological examination revealed local PDL tear at the tension side and the formation of extensive cell-free hyaline zones at the compression side. The immunolocalization of HMGB1 was significantly presented at tension side of PDL, apical area and dental pulp, whereas at the compression side of PDL, the labeling of HMGB1 was almost undetectable as the presence of hyaline zone. Taken together, we concluded that the orthodontic tooth movement by Waldo's method leads to histological changes and HMGB1 expression pattern that differ from those of coil spring method, including PDL tear and extensive hyaline zone which may severely destroy periodontal tissue and in turn impede tooth movement.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Bone Metabolism, School of Stomatology, Shandong University, Wenhua West Road 44-1, Jinan, 250012, China.
authors.forename: Shengyu
authors.initials: S
authors.lastname: Lv
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Feng
authors.forename: Hongrui
authors.initials: H
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Cui
authors.forename: Bao
authors.initials: B
authors.lastname: Sun
authors.forename: Xiuchun
authors.initials: X
authors.lastname: Han
authors.forename: Kimimitsu
authors.initials: K
authors.lastname: Oda
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D015870:Gene Expression; D024243:HMGB1 Protein / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism; D010513:Periodontal Ligament / Q000378:metabolism; D010519:Periodontium / Q000166:cytology* / Q000378:metabolism*; D014086:Tooth Mobility / Q000235:genetics / Q000378:metabolism*
chemical_list: D024243:HMGB1 Protein