title: MiR-200b modulates the properties of human monocyte-derived dendritic cells by targeting WASF3.
abstract: OBJECTIVE
  The aim of the study was to explore the effect of miR-200b on the development of human peripheral blood monocyte-deriveddendritic-cell (DC) and its mechanisms.
  
  
  METHODS
  Expression levels of miR-200b and its predicted targets were measured by real time-PCR. Protein expression of WASF3 was determined by Western blot and immunohistochemistry. Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque density gradient centrifugation from the buffy coat fraction of anticoagulated blood. Monocytes were purified from PBMCs using anti-CD14 microbeads. The immunophenotypes of DCs were tested by flow cytometry.
  
  
  RESULTS
  A strong reduction in miR-200b expression was associated with human peripheral blood monocyte-derivedDC differentiation. The overexpression of miR-200b significantly reduced the numbers of protruding veils in mature DCs (mDCs) that are critical for promoting antigen-specificT-cell activation. Further experiments showed that miR-200b could regulate the function of DCs by targeting WASF3, a protein involved in cell movement and invasion.
  
  
  CONCLUSIONS
  Our results define an important function of miR-200b in the negative regulation of DC development and provide a potential form of miRNA-mediated cell therapy for diseases that range from auto-immunity to graft-versus-host disease.
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Jide
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Xueting
authors.initials: X
authors.lastname: Su
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Xingliang
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ding
authors.affiliation: Laboratory of Computer-aided Drug Design & Discovery, Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Long
authors.initials: L
authors.lastname: Long
authors.affiliation: Laboratory of Computer-aided Drug Design & Discovery, Institute of Pharmacology and Toxicology, Beijing 100850, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Zhanghua
authors.initials: Z
authors.lastname: Li
authors.affiliation: Baoding First Central Hospital, Hebei Province, Baoding 071000, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China. Electronic address: zhangyi612@hotmail.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Beijing 100850, China. Electronic address: shaonsh@hotmail.com.
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D007124:Immunoenzyme Techniques; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D009000:Monocytes / Q000166:cytology*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C120153:WASF3 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family
keywords: Dendritic cell; Human monocyte; MiR-200b; WASF3