title: Association of apolipoprotein A5 genetic polymorphisms with steroid-induced osteonecrosis of femoral head in a Chinese Han population.
abstract: BACKGROUND
  Previous studies suggested that apolipoprotein A5 (ApoA5) genetic polymorphisms (SNPs) may result in lipid metabolism disorders. Therefore, genetic polymorphisms in ApoA5 may be associated with the occurrence of osteonecrosis of femoral head (ONFH).
  
  
  METHODS
  We designed a case-control study including 223 patients of osteonecrosis and 201 age- and sex-matched control subjects to analyze the association between ApoA5 polymorphisms and susceptibility of steroid-induced ONFH. We utilized polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to genotype two SNPs (rs662799 and rs3135506) in ApoA5 gene.
  
  
  RESULTS
  We found both rs662799 and rs3135506 were associated with the risk of ONFH in codominant, dominant, and recessive model, respectively. Haplotype analyses suggested that T-C haplotype was associated with decreased risk of ONFH, whereas the haplotype C-C was significantly associated with an increased risk of ONFH.
  
  
  CONCLUSIONS
  Our study suggested that ApoA5 genetic polymorphisms were associated with susceptibility to ONFH in Chinese population. However, our results need further investigation with large sample size and various populations.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_229.
authors.affiliation: Department of orthopetics, Fifth Affiliated Hospital of Xinjiang Medical University, No.118, Henan West Street, Urumqi, 830011, China. yongcuiig@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of orthopetics, Fifth Affiliated Hospital of Xinjiang Medical University, No.118, Henan West Street, Urumqi, 830011, China. ahemmaitt@yeah.net.
authors.forename: Aihemaiti
authors.initials: A
authors.lastname: Kaisaierjiang
authors.affiliation: Department of orthopetics, Fifth Affiliated Hospital of Xinjiang Medical University, No.118, Henan West Street, Urumqi, 830011, China. ppengggao@163.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of orthopetics, Fifth Affiliated Hospital of Xinjiang Medical University, No.118, Henan West Street, Urumqi, 830011, China. wuzhongyyan@yeah.net.
authors.forename: Zhong-Yan
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: Department of orthopetics, Fifth Affiliated Hospital of Xinjiang Medical University, No.118, Henan West Street, Urumqi, 830011, China. lvvqqing@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lv
mesh_terms: D000328:Adult; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A / Q000235:genetics*; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005271:Femur Head Necrosis / Q000139:chemically induced* / Q000208:ethnology / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013256:Steroids / Q000009:adverse effects*
chemical_list: C437704:APOA5 protein, human; D000072040:Apolipoprotein A-V; D001054:Apolipoproteins A; D013256:Steroids