title: [Repair of xiangsha liujunzi decoction on damage of interstitial cells of cajal and gap junction in the gastric muscular layer of rats of Pi-Qi deficiency syndrome: an experiment study].
abstract: OBJECTIVE
  To explore the repair of Xiangsha Liujunzi Decoction (XSLJZD) on interstitial cells of Cajal (ICC) and gap junction (GJ) in the gastric muscular layer of rats of Pi-qi deficiency syn- drome (PQDS).
  
  
  METHODS
  PQDS was established using purgative method with bitter and cold drugs in 30 healthy Wistar rats. After successful modeling they were randomly divided into the treatment group and the model group, 15 in each group. Another 15 healthy Wistar rats were recruited as the healthy control group. Rats in the treatment group were gastric administered with XSLJZD at 2 mL/100 g body weight, once daily for 14 successive days. Equal volume of normal saline was gastrically administered to those in the healthy control group and the model group. The gastric muscle tissues were taken out before modeling, before intervention, and after intervention, respectively. Ultrastructural changes of ICC and GJ were observed using transmission electron microscope (TEM). The number and distribution of Connexin43 (Cx43) were detected using immunohistochemistry.
  
  
  RESULTS
  Results of TEM indicated that compared with the healthy control group, both ICC and GJ in the model group showed obvious injury. ICC and GJ were apparently repaired after intervention in the treatment group. Compared with the same group before modeling, the integrated optical density (IOD) of the Cx43 expression significantly decreased in the model group before and after intervention (P <0.05). Compared with before intervention, the IOD of the Cx43 expression significantly increased in the treatment group (P <0.05). Compared with the healthy control group, the IOD of the Cx43 expression significantly decreased in the model group before and after intervention (P <0.05). Compared with the model group, the IOD of the Cx43 expression significantly increased in the treatment group (P <0.05).
  
  
  CONCLUSIONS
  Ultrastructures of ICC and GJ in the gastric muscular layer of rats of PQDS were obviously damaged. XSLJZD could repair the structural damage of ICC and GJ in the gastric muscle tissues of rats of PQDS.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.forename: Qing-hui
authors.initials: QH
authors.lastname: Qi
authors.forename: Ming-zhenq
authors.initials: MZ
authors.lastname: Xie
authors.forename: Bo-long
authors.initials: BL
authors.lastname: Ding
authors.forename: Sheng-lin
authors.initials: SL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018031:Connexin 43; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017629:Gap Junctions; D056885:Interstitial Cells of Cajal / Q000187:drug effects*; D007985:Leydig Cells; D008297:Male; D009130:Muscle, Smooth; D019413:Qi; D017208:Rats, Wistar; D013577:Syndrome
chemical_list: D018031:Connexin 43; D004365:Drugs, Chinese Herbal; C521739:liujunzi