title: Proteome alterations in cortex of mice exposed to fluoride and lead.
abstract: Both fluoride and lead can cross the blood-brain barrier and produce toxic effects on the central neural system, resulting in low learning and memory abilities, especially in children. In order to identify the proteomic pattern in the cortex of young animals, from the beginning of fertilization to the age of postnatal day 56, pregnant female mice and pups were administrated with 150 mg sodium fluoride/L and/or 300 mg lead acetate/L in their drinking water. Two-dimensional electrophoresis (2-DE) combined with mass spectrometry (MS) was applied to identify differently expressed protein spots. Results showed that there were eight proteins in the cortex that significantly changed, whose biological functions were involved in (1) energy metabolism (Ndufs1, Atp5h, Atp6v1b2), (2) cytoskeleton (Spna2, Tuba1a, Tubb2a), (3) glycation repair (Hdhd2), and (4) cell stress response (Hspa8). Based on the previous and current studies, ATPase, Spna2, and Hspa8 were shared by fluoride and lead both as common target molecules.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu, Shanxi, China, niuruiyan@sxau.edu.cn.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Niu
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shuangling
authors.initials: S
authors.lastname: Liu
authors.forename: Fengyu
authors.initials: F
authors.lastname: Liu
authors.forename: Zilong
authors.initials: Z
authors.lastname: Sun
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D007854:Lead / Q000494:pharmacology*; D051379:Mice; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics
chemical_list: D020543:Proteome; D007854:Lead; D005459:Fluorides