title: Heterologous expression of highly reducing polyketide synthase involved in betaenone biosynthesis.
abstract: A unique highly reducing polyketide synthase (HR-PKS) with a reductase domain was identified in a betaenone biosynthetic gene cluster. Successful heterologous expression and characterization of the HR-PKS and trans-acting enoyl reductase (ER) provide insights into the core structure formation with a decalin scaffold and allow reconstitution of the betaenone biosynthetic machinery.
authors.affiliation: Division of Chemistry, Graduate School of Science, Hokkaido University, Japan. hoik@sci.hokudai.ac.jp.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ugai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Minami
authors.forename: Ryuya
authors.initials: R
authors.lastname: Fujii
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tanaka
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oguri
authors.forename: Katsuya
authors.initials: K
authors.lastname: Gomi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Oikawa
mesh_terms: D000475:Alkenes / Q000737:chemistry / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005800:Genes, Fungal / Q000235:genetics; D007659:Ketones / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D005810:Multigene Family; D012275:Oryza / Q000235:genetics; D048630:Polyketide Synthases / Q000235:genetics*; D017434:Protein Structure, Tertiary
chemical_list: D000475:Alkenes; D007659:Ketones; D048630:Polyketide Synthases