title: Highly selective colorimetric bacteria sensing based on protein-capped nanoparticles.
abstract: A rapid and cost-effective colorimetric sensor has been developed for the detection of bacteria (Bacillus subtilis was selected as an example). The sensor was designed to rely on lysozyme-capped AuNPs with the advantages of effective amplification and high specificity. In the sensing system, lysozyme was able to bind strongly to Bacillus subtilis, which effectively induced a color change of the solution from light purple to purplish red. The lowest concentration of Bacillus subtilis detectable by the naked eye was 4.5 × 10(3) colony-forming units (CFU) mL(-1). Similar results were discernable from UV-Vis absorption measurements. A good specificity was observed through a statistical analysis method using the SPSS software (version 17.0). This simple colorimetric sensor may therefore be a rapid and specific method for a bacterial detection assay in complex samples.
authors.affiliation: Institute for Quality & Safety and Standards of Agricultural Products Research, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi 330200, China. luolinguang@126.com.
authors.forename: Suyan
authors.initials: S
authors.lastname: Qiu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Yaomin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Donggen
authors.initials: D
authors.lastname: Wang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yuan
authors.forename: Yihua
authors.initials: Y
authors.lastname: Wei
authors.forename: Tingcan
authors.initials: T
authors.lastname: Dai
authors.forename: Linguang
authors.initials: L
authors.lastname: Luo
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D001412:Bacillus subtilis / Q000302:isolation & purification*; D015169:Colony Count, Microbial / Q000191:economics / Q000379:methods*; D003124:Colorimetry / Q000191:economics / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding
chemical_list: D004800:Enzymes, Immobilized; D009113:Muramidase