title: Differential Expression of Long Noncoding RNAs in Human Cumulus Cells Related to Embryo Developmental Potential: A Microarray Analysis.
abstract: Long noncoding RNAs (lncRNAs), which are prevalently transcribed in the genome, are involved in a variety of biological functions, yet little is known about their abundance in human cumulus cells (CCs) during oocyte development. Here, we describe the expression profile of lncRNAs in 3 pairs of cumulus cells from mature oocytes that result in high-quality embryo (H-CCs) and from oocytes that result in poor-quality embryo (P-CCs) using microarray analysis. In this study, a total of 20 563 lncRNAs were expressed in human CCs. One hundred and twenty four lncRNAs were consistently upregulated, and 509 lncRNAs were consistently downregulated in all samples analyzed (fold change ≥ 2.0, P < .05). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate 5 upregulated and 7 downregulated lncRNAs. The qRT-PCR results in the study were confirmed to be consistent with the microarray results. Network analysis was used for further research. The results displayed the differentially expressed lncRNAs in P-CCs between H-CCs, which suggested that lncRNAs may contribute to the processes of oocyte and early embryo development.
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Xu
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China caoyunxia6@126.com.
authors.forename: Yun-Xia
authors.initials: YX
authors.lastname: Cao
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Chen
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Zhang
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Xiao-Jin
authors.initials: XJ
authors.lastname: He
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Ji
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital, Anhui Medical University, Hefei, China Institute of Reproductive Genetics, The First Affiliated Hospital, Anhui Medical University, Hefei, China.
authors.forename: Bei-Li
authors.initials: BL
authors.lastname: Chen
mesh_terms: D000328:Adult; D016000:Cluster Analysis; D054885:Cumulus Cells / Q000378:metabolism*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D005819:Genetic Markers; D062085:RNA, Long Noncoding
keywords: cumulus cells; embryo quality; long noncoding RNA; microarray