title: Poly(ε-caprolactone) films with favourable properties for neural cell growth.
abstract: The regeneration of brain tissue is one of the major challenges in regenerative medicine due to the lack of viable grafts to support the re-growth of functional tissue after a traumatic injury. The development of biocompatible and biodegradable structures with appropriate morphology for the interaction with neural tissue is required. The objective pursued in this work is to develop a biodegradable 2D scaffold structure for neural tissue engineering. Poly(ε-caprolactone) (PCL) was the selected material due to its biocompatibility and biodegradability in the long term. PCL (15%w/w) was dissolved in N-methylpyrrolidone and the film was fabricated by phase inversion casting technique employing ethanol and isopropanol as coagulation baths. The physical structure, morphology and topography of the flat scaffolds were characterized using different techniques. The two different scaffolds presented homogeneous structure with high porosity (higher than 85%), contact angles higher than 90(o), high roughness (Ra> 0.6 μm) and superficial pore sizes of 0.7 and 1.7 μm, respectively. Permeance tests showed high water permeabilities (~350-590 mL m(-1) bar(-1) h(-1)) indicative of promising nutrients supply to the cells. Finally, in vitro human glioblastoma cells cultures after 48 hours showed good cell attachment, proliferation and penetration in the scaffolds. Detailed evaluation of the interaction between the surface morphology and the properties of the scaffolds with the cell response has been done. Thus, the PCL films herein fabricated show promising results as scaffolds for neural tissue regeneration.
authors.forename: N
authors.initials: N
authors.lastname: Diban
authors.forename: J
authors.initials: J
authors.lastname: Ramos-Vivas
authors.forename: S
authors.initials: S
authors.lastname: Remuzgo-Martinez
authors.forename: I
authors.initials: I
authors.lastname: Ortiz
authors.affiliation: Department of Chemical and Biomolecular Engineering. University of Cantabria. Av. de los Castros s/n. 39005 Santander, Spain. dibann@unican.es.
authors.forename: A
authors.initials: A
authors.lastname: Urtiaga
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D009416:Nerve Regeneration; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010539:Permeability; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology*; D016062:Porosity; D011760:Pyrrolidinones; D012997:Solvents; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014867:Water / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; D011760:Pyrrolidinones; D012997:Solvents; D014867:Water; C016240:polycaprolactone; C038678:N-methylpyrrolidone