title: Cyclic AMP in oocytes controls meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary.
abstract: In mammalian ovaries, a fixed population of primordial follicles forms during the perinatal stage and the oocytes contained within are arrested at the dictyate stage of meiotic prophase I. In the current study, we provide evidence that the level of cyclic AMP (cAMP) in oocytes regulates oocyte meiotic prophase I and primordial folliculogenesis in the perinatal mouse ovary. Our results show that the early meiotic development of oocytes is closely correlated with increased levels of intra-oocyte cAMP. Inhibiting cAMP synthesis in fetal ovaries delayed oocyte meiotic progression and inhibited the disassembly and degradation of synaptonemal complex protein 1. In addition, inhibiting cAMP synthesis in in vitro cultured fetal ovaries prevented primordial follicle formation. Finally, using an in situ oocyte chromosome analysis approach, we found that the dictyate arrest of oocytes is essential for primordial follicle formation under physiological conditions. Taken together, these results suggest a role for cAMP in early meiotic development and primordial follicle formation in the mouse ovary.
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Yijing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Teng
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510663, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China Department of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Zhengpin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Lizhao
authors.initials: L
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Wanbao
authors.initials: W
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg SE-405 30, Sweden hua.zhang@gu.se glxiachina@sohu.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agrobiotechnology, College of Biological Science, China Agricultural University, Beijing 100193, China hua.zhang@gu.se glxiachina@sohu.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xia
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D049469:Meiotic Prophase I / Q000502:physiology*; D051379:Mice; D042282:Microdissection; D009865:Oocytes / Q000378:metabolism*; D038081:Organogenesis / Q000502:physiology*; D006080:Ovarian Follicle / Q000196:embryology*; D034622:RNA Interference; D011863:Radioimmunoassay
chemical_list: D000242:Cyclic AMP
keywords: Meiotic prophase I; Oocytes; Primordial follicle formation; cAMP