title: Polysaccharides from the green seaweed Codium decorticatum. Structure and cell wall distribution.
abstract: The cell wall polysaccharides from Codium decorticatum and their assembly were studied and these results were compared with those obtained previously for this genus. The water soluble polysaccharides are: (i) Pyruvylated and sulfated 3- and 6-linked β-D-galactans with sulfate mainly on C-4 and also on C-6. Pyruvate ketals are linked to O-3 and O-4 of terminal β-D-galactose or O-4 and O-6 of 3-linked β-D-galactose. (ii) Sulfated 3-linked β-L-arabinans substituted on C-2 or C-2 and C-4 predominantly with sulfate, but also with single stubs of arabinose, and (iii) 4-linked β-D-mannans with a low degree of sulfation on C-2. The whole polysaccharide system comprises 6.9% of sulfated polysaccharides and 32.9% of fibrillar polysaccharides, mostly insoluble mannans. By in situ localization it was possible to detect two similar fibrillar layers separated by a zone rich in charged polymers. Besides, arabinogalactan proteins co-localized with the fibrillar components.
authors.affiliation: Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, 1417 Buenos Aires, Argentina; CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina.
authors.forename: Paula Virginia
authors.initials: PV
authors.lastname: Fernández
authors.affiliation: Centro Nacional Patagónico-CONICET, Bvd. Brown 2915, U9120ACD Puerto Madryn, Chubut, Argentina.
authors.forename: María Paula
authors.initials: MP
authors.lastname: Raffo
authors.affiliation: Departamento de Ecología Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina.
authors.forename: Josefina
authors.initials: J
authors.lastname: Alberghina
authors.affiliation: Cátedra de Química de Biomoléculas, Departamento de Biología Aplicada y Alimentos, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, 1417 Buenos Aires, Argentina; CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, 1428 Buenos Aires, Argentina. Electronic address: ciancia@agro.uba.ar.
authors.forename: Marina
authors.initials: M
authors.lastname: Ciancia
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D000460:Chlorophyta / Q000737:chemistry* / Q000166:cytology*; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism*; D012624:Seaweed / Q000737:chemistry* / Q000166:cytology*
chemical_list: D011134:Polysaccharides
keywords: Cell wall; Codium; Green seaweed; Polysaccharide structure; Sulfated polysaccharide