title: Eicosanoids mediate sHSP 20.8 gene response to biotic stress in larvae of the Chinese oak silkworm Antheraea pernyi.
abstract: Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi (A. pernyi; Lepidoptera: Saturniidae), cDNA encoding HSP20.8 in A. pernyi, termed Ap-sHSP20.8, was identified as a 564 bp ORF. The translated amino acid sequence encoded 187 residues with a calculated molecular mass of 20.8 kDa and an isoelectronic point (pI) of 5.98; the sequence showed homology to sHSP chaperone proteins from other insects. Ap-sHSP20.8 mRNA transcript expression was abundant in the midgut and fat body and found to be both constitutive and inducible by infectious stimuli. Therefore, Ap-sHSP20.8 may play important roles in A. pernyi immune responses under biotic stress. Furthermore, we found that eicosanoids could mediate the induction of Ap-sHSP20.8 in the fat body and midgut. Our findings show that sHSPs may be promising molecules to target in order to cripple immunity in insect pests.
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China; Department of Pharmacology, Wannan Medical College, Anhui, Wuhu 241002, PR China.
authors.forename: Cong-Fen
authors.initials: CF
authors.lastname: Zhang
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China.
authors.forename: Li-Shang
authors.initials: LS
authors.lastname: Dai
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Qian
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Wei
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China. Electronic address: zhubaojian@ahau.edu.cn.
authors.forename: Bao-Jian
authors.initials: BJ
authors.lastname: Zhu
authors.affiliation: College of Life Science, Anhui Agricultural University, Anhui, Hefei 230036, PR China. Electronic address: cyschx@163.com.
authors.forename: Chao-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D020171:Botrytis / Q000276:immunology; D015777:Eicosanoids / Q000276:immunology / Q000494:pharmacology; D004926:Escherichia coli / Q000276:immunology; D005216:Fat Body / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D018507:Gene Expression Regulation, Developmental; D050888:Heat-Shock Proteins, Small / Q000235:genetics* / Q000276:immunology; D019476:Insect Proteins / Q000235:genetics* / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects / Q000276:immunology / Q000382:microbiology; D007526:Isoelectric Point; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000276:immunology; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000276:immunology; D017924:Nucleopolyhedroviruses / Q000276:immunology; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological
chemical_list: D015777:Eicosanoids; D050888:Heat-Shock Proteins, Small; D019476:Insect Proteins
keywords: Antheraea pernyi; Eicosanoids; Small heat shock protein 20.8