title: Crystallization: digging into the past to learn lessons for the future.
abstract: Crystals of biological macromolecules have been observed and grown for well over a century. More effort has been put into biological crystallization in the last few decades due to the importance of X-ray crystal structures, the advent of synchrotron radiation sources, improved computational speed, better software, and the availability of recombinant protein. Here we focus on two important areas of crystal growth: firstly, on techniques for stabilizing the protein sample, and secondly, on strategies and approaches for selecting the crystallization cocktails most suitable for different strategies.
authors.affiliation: Collaborative Crystallisation Centre, CSIRO Biomedical Manufacturing, 343 Royal Parade, Parkville, VIC, 3052, Australia.
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Fazio
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
mesh_terms: D018360:Crystallography, X-Ray / Q000295:instrumentation / Q000639:trends*; D030562:Databases, Protein; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry*; D012371:Robotics; D017356:Synchrotrons
chemical_list: D011506:Proteins