title: Autologous transplantation of CD34(+) bone marrow derived mononuclear cells in management of non-reconstructable critical lower limb ischemia.
abstract: Patients with a decrease in limb perfusion with a potential threat to limb viability manifested by ischemic rest pain, ischemic ulcers, and/or gangrene are considered to have critical limb ischemia (CLI). Because of this generally poor outcome, there is a strong need for attempting any procedure to save the affected limb. The aim of this work is to evaluate the possibility to use stem cell therapy as a treatment option for patients with chronic critical lower limb ischemia with no distal run off. This study includes 20 patients with chronic critical lower limb ischemia with no distal run off who are unsuitable for vascular or endovascular option. These patients underwent stem cell therapy (SCT) by autologous transplantation of bone marrow derived mononuclear cells. 55 % of patients treated with SCT showed improvement of the rest pain after the first month, 60 % continued improvement of the rest pain after 6 months, 75 % after 1 year and 80 % after 2 years and continued without any deterioration till the third year. Limb salvage rate after STC was 80 % after the first year till the end of the second and third years. SCT can result in angiogenesis in patients with no-option CLI, providing a foundation for the application of this therapy to leg ischemia.
authors.affiliation: Vascular Surgery Unit, Tanta University, Tanta, Egypt.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Ismail
authors.affiliation: Clinical Pathology Department, Tanta University, Tanta, Egypt.
authors.forename: Said M
authors.initials: SM
authors.lastname: Abdou
authors.affiliation: Vascular Surgery Unit, Tanta University, Tanta, Egypt.
authors.forename: Hassan Abdel
authors.initials: HA
authors.lastname: Aty
authors.affiliation: Vascular Surgery Unit, Tanta University, Tanta, Egypt.
authors.forename: Adel H
authors.initials: AH
authors.lastname: Kamhawy
authors.affiliation: Vascular Surgery Unit, Tanta University, Tanta, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Elhinedy
authors.affiliation: Vascular Surgery Unit, Tanta University, Tanta, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Elwageh
authors.affiliation: Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Atef
authors.initials: A
authors.lastname: Taha
authors.affiliation: Clinical Pathology Department, Tanta University, Tanta, Egypt.
authors.forename: Amal
authors.initials: A
authors.lastname: Ezzat
authors.affiliation: Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
authors.forename: Hoda A
authors.initials: HA
authors.lastname: Salem
authors.affiliation: Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Said
authors.initials: S
authors.lastname: Youssif
authors.affiliation: Immunology and Biotechnology Unit, Zoology Department, Faculty of Science, Center of Excellence in Cancer Research, Tanta University, Tanta, Egypt. Mohamed.labib@science.tanta.edu.eg.
authors.forename: Mohamed L
authors.initials: ML
authors.lastname: Salem
keywords: Bone marrow; CD34+ cells; G-CSF; Leg ischemia; Mobilization