title: Live cell interferometry quantifies dynamics of biomass partitioning during cytokinesis.
abstract: The equal partitioning of cell mass between daughters is the usual and expected outcome of cytokinesis for self-renewing cells. However, most studies of partitioning during cell division have focused on daughter cell shape symmetry or segregation of chromosomes. Here, we use live cell interferometry (LCI) to quantify the partitioning of daughter cell mass during and following cytokinesis. We use adherent and non-adherent mouse fibroblast and mouse and human lymphocyte cell lines as models and show that, on average, mass asymmetries present at the time of cleavage furrow formation persist through cytokinesis. The addition of multiple cytoskeleton-disrupting agents leads to increased asymmetry in mass partitioning which suggests the absence of active mass partitioning mechanisms after cleavage furrow positioning.
authors.affiliation: Department of Bioengineering, University of California Los Angeles (UCLA), Los Angeles, California, United States of America; Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, California, United States of America.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Zangle
authors.affiliation: Department of Bioengineering, University of California Los Angeles (UCLA), Los Angeles, California, United States of America; Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, California, United States of America; Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America; California NanoSystems Institute, UCLA, Los Angeles, California, United States of America; Broad Stem Cell Research Center, UCLA, Los Angeles, California, United States of America; Molecular Biology Institute, UCLA, Los Angeles, California, United States of America.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Teitell
authors.affiliation: Department of Physics, Virginia Commonwealth University (VCU), Richmond, Virginia, United States of America; VCU Massey Cancer Center, Richmond, Virginia, United States of America.
authors.forename: Jason
authors.initials: J
authors.lastname: Reed
mesh_terms: D000818:Animals; D018533:Biomass; D048430:Cell Shape; D058948:Cell Tracking; D002478:Cells, Cultured; D048749:Cytokinesis / Q000187:drug effects / Q000502:physiology*; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007368:Interferometry / Q000379:methods*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000502:physiology; D009101:Multiple Myeloma / Q000188:drug therapy / Q000473:pathology*; D008941:Spindle Apparatus / Q000187:drug effects / Q000502:physiology; D050257:Tubulin Modulators / Q000494:pharmacology*
chemical_list: D050257:Tubulin Modulators