title: Distribution of constitutive heterochromatin in species of triatomines with fragmentation of sex chromosomes X.
abstract: Cytogenetic analyses of triatomines are considered to be important taxonomic tools. Thus, we analyzed the pattern of constitutive heterochromatin in 7 species of triatomine with fragmentation of the sex chromosome X, focusing on the cytotaxonomy of these triatomines. The species analyzed included Triatoma vitticeps, Triatoma melanocephala, Triatoma tibiamaculata, Triatoma protracta, Meccus pallidipennis, Panstrongylus megistus, and Panstrongylus lignarius. The seminiferous tubules of the adult males were subjected to C-banding. P. megistus and P. lignarius showed differences in chromosome number and disposition of constitutive heterochromatin, as only P. lignarius showed C-blocks in autosomes. C-banding can differentiate these species, since one of the sex chromosome (X) is heterochromatic in T. vitticeps. T. protracta showed C-blocks in both ends of all autosomes, T. tibiamaculata showed terminal C-dots in some autosomal pairs and M. pallidipennis did not show constitutive heterochromatin in autosomes. Thus, we confirmed the heterochromatic pattern of 7 species of insects and emphasized the importance of cytogenetic techniques for C-banding for taxonomy studies of the triatomines, which are important vectors of Chagas disease.
authors.affiliation: Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brasil.
authors.forename: A L
authors.initials: AL
authors.lastname: Guerra
authors.affiliation: Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brasil kaiochaboli@hotmail.com.
authors.forename: K C C
authors.initials: KC
authors.lastname: Alevi
authors.affiliation: Laboratório de Parasitologia, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista "Júlio de Mesquita Filho", Araraquara, SP, Brasil.
authors.forename: J A
authors.initials: JA
authors.lastname: Rosa
authors.affiliation: Laboratório de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brasil.
authors.forename: M T V
authors.initials: MT
authors.lastname: Azeredo-Oliveira
mesh_terms: D000818:Animals; D002871:Chromosome Banding / Q000379:methods; D059006:Chromosomes, Insect / Q000378:metabolism*; D006570:Heterochromatin / Q000378:metabolism*; D008297:Male; D012671:Seminiferous Tubules / Q000378:metabolism; D013045:Species Specificity; D014225:Triatominae / Q000145:classification* / Q000235:genetics*; D014960:X Chromosome / Q000378:metabolism*
chemical_list: D006570:Heterochromatin