title: A 2-DE-based proteomic study on the toxicological effects of cisplatin in L02 cells.
abstract: Cisplatin is a chemotherapeutic agent for the treatment of various cancers. In this study, cisplatin-induced effects were characterized in vitro model of human liver cells (L02) using 2-DE-based proteomics. Results indicated that different cisplatin treatments primarily induced disturbances in protein synthesis and oxidative stress via differential mechanisms. Since the experimental concentrations of cisplatin described a hormesis effect in cell proliferation of L02 cells, it was expected to reveal the hormesis effects using proteomic markers. However, only confilin-1 was commonly up-regulated in three concentrations of cisplatin treatments showing a hormesis effects with a U-shape regulation. These results were highly consistent with many other toxico-proteomic studies, indicating that the toxico-proteomic responses based on dose-dependent protein responses were incongruent with the theoretically linear or hormetic concentration-effect relationship. Our findings suggested that a macroscopic hormesis phenomenon on the cell proliferation could not be reflected by proteomic responses induced by cisplatin treatments.
authors.affiliation: The 2nd People's Hospital of Huaian, No. 62, Huaihainan Road, Huaian 223002, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Liu
authors.affiliation: The 2nd People's Hospital of Huaian, No. 62, Huaihainan Road, Huaian 223002, PR China. Electronic address: weiwanghuaian2013@gmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: The 2nd People's Hospital of Huaian, No. 62, Huaihainan Road, Huaian 223002, PR China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The 2nd People's Hospital of Huaian, No. 62, Huaihainan Road, Huaian 223002, PR China.
authors.forename: Zongli
authors.initials: Z
authors.lastname: Ding
authors.affiliation: The 2nd People's Hospital of Huaian, No. 62, Huaihainan Road, Huaian 223002, PR China.
authors.forename: Runhuan
authors.initials: R
authors.lastname: Gu
mesh_terms: D000970:Antineoplastic Agents / Q000633:toxicity*; D002460:Cell Line; D002945:Cisplatin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D040901:Proteomics
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin
keywords: Cisplatin; L02 cell; Proteomics; Toxicological effects; Two-dimensional electrophoresis