title: A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge.
abstract: Neutral and acidic polysaccharides, named AMP40N and AMP40S respectively, were isolated and purified from the dried bulbs of Allium macrostemon Bunge. Both of them showed a single and symmetrically sharp peak, indicating they were homogeneous polysaccharides. Molecular weights of AMP40N and AMP40S were determined to be 18.2 and 105.1 kDa, respectively. AMP40N was composed of arabinose and glucose, while AMP40S was composed of rhamnose, arabinose, glucose and galactose and a certain amount of uronic acid. FT-IR, periodic acid oxidation, Smith degradation, methylation and GC-MS analysis revealed that non-reducing terminal and →2,6)-Glc-(1→ existed in AMP40N and AMP40S. The glycosidic linkage of arabinose in AMP40N was →2)-Ara-(1→, whereas it was Ara-(1→ in AMP40S. AMP40S had (1→2)-linked l-rhamnose residue. Both AMP40N and AMP40S exhibited strong anti-tumor potential against human gastric carcinoma cells BGC-823, in particular, AMP40S presented significantly higher inhibitory rate of 85.94% than AMP40N of 52.63%.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China; College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou 225009, PR China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Chemical Engineering, Yangzhou Polytechnic Institute, Yangzhou 225127, PR China.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Mingchun
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: zengxx@njau.edu.cn.
authors.forename: Xiaoxiong
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000490:Allium / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008745:Methylation; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides
keywords: Allium macrostemon Bunge; Antitumor activity; Characterization; Polysaccharides