title: Optimization of ultrasound-assisted compound enzymatic extraction and characterization of polysaccharides from blackcurrant.
abstract: In the present study, an efficient procedure for ultrasound-assisted compound enzymatic extraction of polysaccharides from blackcurrant fruits was investigated using response surface methodology (RSM). The Box-Behnken design was applied to optimize the effects of enzyme concentration (X1), pH (X2) and ultrasonic time (X3). The statistical analysis indicated that the independent variables (X1) and the quadratic terms (X1(2) and X3(2)) had significant effects on the yield of blackcurrant polysaccharides (BCP). The optimal conditions were: enzyme concentration 1.575%, pH 5.3, and ultrasonic time 25.6 min. The experimental yield of BCP was 14.28±0.06%, which was closely matched with the predicted yield of 14.31%. After preliminary purification, BCP I was obtained and characterized by GC, HPLC, and IR. BCP I comprised rhamnose, arabinose, xylose, mannose, glucose, and galactose in a molar ratio of 1.818:1.362:0.377:0.501:1.581:1.722 and its molecular weight was 8146 kDa. BCP I showed notable α-amylase inhibitory activity.
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China. Electronic address: xu-yaqin@163.com.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Science, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Horticulture, Northeast Agricultural University, Harbin 150030, China.
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Yu
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D008970:Molecular Weight; D010206:Papain / Q000378:metabolism*; D011096:Polygalacturonase / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D031965:Ribes / Q000737:chemistry*; D013997:Time Factors; D014465:Ultrasonics; D000516:alpha-Amylases / Q000037:antagonists & inhibitors
chemical_list: D004791:Enzyme Inhibitors; D011134:Polysaccharides; D000516:alpha-Amylases; D011096:Polygalacturonase; D010206:Papain
keywords: Acarbose (PubChem CID: 41774); Blackcurrant polysaccharides; Characterization; Optimization; Pectin (PubChem CID: 11883891); Response surface methodology; Trifluoroacetic acid (PubChem CID: 6422); Ultrasound-assisted compound enzymatic extraction; d-Galacturonic acid (PubChem CID: 84740); d-Glucose (PubChem CID: 5793); α-Amylase inhibitory activity