title: Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa.
abstract: The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the bright "on" and dark "off" states via photoisomerization and proton transfer reactions. We report the room temperature crystal structure of the fast switching Met159Thr mutant of Dronpa at 2.0-Å resolution in the bright on state. Structural differences with the wild type include shifted backbone positions of strand β8 containing Thr159 as well as an altered A-C dimer interface involving strands β7, β8, β10, and β11. The Met159Thr mutation increases the cavity volume for the p-hydroxybenzylidene-imidazolinone chromophore as a result of both the side chain difference and the backbone positional differences.
authors.affiliation: Division of Molecular Biosciences, Imperial College London, London, SW7 2AZ, United Kingdom.
authors.forename: Marius
authors.initials: M
authors.lastname: Kaucikas
authors.forename: Ann
authors.initials: A
authors.lastname: Fitzpatrick
authors.forename: Elana
authors.initials: E
authors.lastname: Bryan
authors.forename: Abelone
authors.initials: A
authors.lastname: Struve
authors.forename: Robert
authors.initials: R
authors.lastname: Henning
authors.forename: Irina
authors.initials: I
authors.lastname: Kosheleva
authors.forename: Vukica
authors.initials: V
authors.lastname: Srajer
authors.forename: Gerrit
authors.initials: G
authors.lastname: Groenhof
authors.forename: Jasper J
authors.initials: JJ
authors.lastname: Van Thor
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000235:genetics; D018360:Crystallography, X-Ray; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D009154:Mutation; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D008164:Luminescent Proteins; D011994:Recombinant Proteins
keywords: Dronpa; X-ray structure; molecular dynamics; reversibly photoswitchable fluorescent protein; room temperature