title: Attraction of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) to four varieties of Lathyrus sativus L. seed volatiles.
abstract: Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) is an important stored grain pest of Lathyrus sativus L. (Leguminosae), commonly known as khesari, in India, Bangladesh and Ethiopia. Volatiles were collected from four varieties, i.e., Bio L 212 Ratan, Nirmal B-1, WBK-14-7 and WBK-13-1 of uninfested khesari seeds, and subsequently identified and quantified by gas chromatography mass spectrometry and gas chromatography flame ionization detector analyses, respectively. A total of 23 volatiles were identified in the four varieties of khesari seeds. In Bio L 212 Ratan and WBK-13-1 seeds, nonanal was the most abundant followed by farnesyl acetone; whereas farnesyl acetone was predominant followed by nonanal in Nirmal B-1 and WBK-14-7 khesari seeds. The olfactory responses of female C. maculatus toward volatile blends from four varieties of khesari seeds, and individual synthetic compounds and their combinations were examined through Y-shaped glass tube olfactometer bioassays. Callosobruchus maculatus showed significant preference for the whole volatile blends from Bio L 212 Ratan seeds compared to whole volatile blends from other three varieties. The insect exhibited attraction to five individual synthetic compounds, 3-octanone, 3-octanol, linalool oxide, 1-octanol and nonanal. A synthetic blend of 448, 390, 1182, 659 and 8114 ng/20 μl methylene chloride of 3-octanone, 3-octanol, linalool oxide, 1-octanol and nonanal, respectively, was most attractive to C. maculatus, and this combination might be used for insect pest management program such as baited traps.
authors.affiliation: Ecology Research Laboratory,Department of Zoology,The University of Burdwan,Burdwan - 713 104,West Bengal,India.
authors.forename: P
authors.initials: P
authors.lastname: Adhikary
authors.affiliation: Ecology Research Laboratory,Department of Zoology,The University of Burdwan,Burdwan - 713 104,West Bengal,India.
authors.forename: A
authors.initials: A
authors.lastname: Mukherjee
authors.affiliation: Ecology Research Laboratory,Department of Zoology,The University of Burdwan,Burdwan - 713 104,West Bengal,India.
authors.forename: A
authors.initials: A
authors.lastname: Barik
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001517:Coleoptera; D005260:Female; D027807:Lathyrus / Q000737:chemistry*; D008297:Male; D012639:Seeds / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000494:pharmacology*
chemical_list: D055549:Volatile Organic Compounds
keywords: Y-shaped olfactometer bioassay