title: Sinapic acid protects heart against ischemia/reperfusion injury and H9c2 cardiomyoblast cells against oxidative stress.
abstract: The present study was designed to evaluate antioxidant and cardioprotective potential of sinapic acid (SA) against ischemia/reperfusion (I/R) injury. Cardiac functional recovery after I/R was evaluated by percentage rate pressure product (%RPP) and percentage coronary flow (%CF). Myocardial injury was evaluated by 2,3,5-triphenyltetrazolium chloride (TTC) staining and LDH enzyme leakage. Oxidative stress was estimated by lipid peroxidation level. eNOS protein expression in reperfused heart was assessed using Western blot method. Finally, in order to support the antioxidant effect of SA, in vitro protective potential of SA was assessed on H2O2-induced oxidative stress in H9c2 cardiomyoblast cells. The overall results demonstrated that I/R induced cardiac dysfunction, injury and oxidative stress was attenuated by SA treatment. Moreover, in vitro results also shown that, SA protects H9c2 cells from oxidative stress and modulates mitochondrial membrane permeability transition (MPT). In conclusion, coupled results from both in vivo and in vitro experiments have confirmed that SA with antioxidant role protects cardiac cells and its functions from I/R induced oxidative stress.
authors.affiliation: Cardiovascular Biology Lab, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India.
authors.forename: Thangarasu
authors.initials: T
authors.lastname: Silambarasan
authors.affiliation: Cardiovascular Biology Lab, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India; Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, Tamil Nadu, India.
authors.forename: Jeganathan
authors.initials: J
authors.lastname: Manivannan
authors.affiliation: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, Tamil Nadu, India.
authors.forename: Mani Krishna
authors.initials: MK
authors.lastname: Priya
authors.affiliation: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, Tamil Nadu, India.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Suganya
authors.affiliation: Vascular Biology Lab, AU-KBC Research Centre, Anna University, Chennai, Tamil Nadu, India; Department of Biotechnology, Anna University, Chennai, Tamil Nadu, India.
authors.forename: Suvro
authors.initials: S
authors.lastname: Chatterjee
authors.affiliation: Cardiovascular Biology Lab, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India. Electronic address: drrajaau@gmail.com.
authors.forename: Boobalan
authors.initials: B
authors.lastname: Raja
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D003373:Coumaric Acids / Q000494:pharmacology / Q000627:therapeutic use*; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D032446:Myoblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D009203:Myocardial Infarction / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D003373:Coumaric Acids; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; C073734:sinapinic acid; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D052250:Nitric Oxide Synthase Type III
keywords: Cardioprotective; Ischemia/reperfusion; Myocardial injury; Oxidative stress; Sinapic acid