title: African swine fever virus CD2v and C-type lectin gene loci mediate serological specificity.
abstract: African swine fever (ASF) is an emerging disease threat for the swine industry worldwide. No ASF vaccine is available and progress is hindered by lack of knowledge concerning the extent of ASF virus (ASFV) strain diversity and the viral antigens responsible for protection in the pig. Available data from vaccination/challenge experiments in pigs indicate ASF protective immunity is haemadsorption inhibition (HAI) serotype-specific. A better understanding of ASFV HAI serological groups and their diversity in nature, as well as improved methods to serotype ASFV isolates, is needed. Here, we demonstrated that the genetic locus encoding ASFV CD2v and C-type lectin proteins mediates HAI serological specificity and that CD2v/C-type lectin genotyping provides a simple method to group ASFVs by serotype, thus facilitating study of ASFV strain diversity in nature, and providing information necessary for eventual vaccine design, development and efficacious use.
authors.affiliation: National Research Institute for Veterinary Virology and Microbiology, Russian Academy of Agriculture Science, Pokrov, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Malogolovkin
authors.affiliation: National Research Institute for Veterinary Virology and Microbiology, Russian Academy of Agriculture Science, Pokrov, Russia.
authors.forename: G
authors.initials: G
authors.lastname: Burmakina
authors.affiliation: Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, Storrs, CT, USA.
authors.forename: E R
authors.initials: ER
authors.lastname: Tulman
authors.affiliation: School of Veterinary Medicine & Biomedical Sciences and Nebraska Center for Virology, University of Nebraska, Lincoln, NE, USA.
authors.forename: G
authors.initials: G
authors.lastname: Delhon
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL, USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Diel
authors.affiliation: National Research Institute for Veterinary Virology and Microbiology, Russian Academy of Agriculture Science, Pokrov, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Salnikov
authors.affiliation: Department of Pathobiology and Veterinary Science and Center of Excellence for Vaccine Research, University of Connecticut, Storrs, CT, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Kutish
authors.affiliation: National Research Institute for Veterinary Virology and Microbiology, Russian Academy of Agriculture Science, Pokrov, Russia.
authors.forename: D
authors.initials: D
authors.lastname: Kolbasov
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana, IL, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Rock
mesh_terms: D000358:African Swine Fever Virus / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D056426:Genetic Loci; D005838:Genotype; D059810:HLA-DR Serological Subtypes / Q000235:genetics*; D037181:Lectins, C-Type / Q000235:genetics* / Q000276:immunology; D012680:Sensitivity and Specificity; D013552:Swine; D014764:Viral Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D059810:HLA-DR Serological Subtypes; D037181:Lectins, C-Type; D014764:Viral Proteins; D014765:Viral Vaccines