title: Multiple antigenic polypeptide composed of heparanase B‑cell epitopes shrinks human hepatocellular carcinoma in mice.
abstract: The purpose of this study was to evaluate the anti‑growth effect of the self‑designed multiple antigenic polypeptide (MAP) vaccine comprising B‑cell epitopes of heparanase (HPSE) on HCC97‑H hepatocellular carcinoma (HCC) in mice. The polyclonal antibodies against the B‑cell epitopes of HPSE were prepared by immunizing rabbits with freshly synthesized MAP vaccine. HCC‑bearing models were constructed on BALB/c nude mice. Anti‑MAP antibodies were administrered to the models to assess the effects on HPSE activity, HCC growth, the expression of VEGF/bFGF and the value of micro‑vessel density (MVD). The anti‑MAP antibodies were harvested, purified and identified. These antibodies were able to specifically bind with the dominant epitopes of the precursor protein and large subunit monomer of HPSE, decrease HPSE activity, suppress the expressions of VEGF and bFGF, reduce the MVD, and markedly shrink the HCC volume. Based on these findings, MAP vaccine based on the B‑cell epitopes of HPSE seemed to provide theoretical evidence for further study of the synthesized HPSE MAP vaccine in the treatment of HCC.
authors.affiliation: Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Sir Run Run Shaw Hospital, Hangzhou, Zhejiang 310016, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Intensive Care Medicine, The Central Hospital of Jinhua City, Jinhua, Zhejiang 321001, P.R. China.
authors.forename: Ni
authors.initials: N
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Huiju
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China.
authors.forename: Tong
authors.initials: T
authors.lastname: Yu
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000627:therapeutic use*; D000915:Antibody Affinity / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000628:therapy*; D045744:Cell Line, Tumor; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D005346:Fibroblast Growth Factors / Q000096:biosynthesis / Q000097:blood; D005966:Glucuronidase / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D008113:Liver Neoplasms / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D055806:Microvessels / Q000378:metabolism; D009368:Neoplasm Transplantation; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D014183:Transplantation, Heterologous; D022223:Vaccines, Subunit / Q000276:immunology / Q000627:therapeutic use; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000097:blood; D000099175:Heparanase
chemical_list: D000906:Antibodies; D019496:Cancer Vaccines; D018985:Epitopes, B-Lymphocyte; D005346:Fibroblast Growth Factors; D005966:Glucuronidase; D010455:Peptides; D022223:Vaccines, Subunit; D042461:Vascular Endothelial Growth Factor A; D000099175:Heparanase; C467480:vascular endothelial growth factor A, mouse