title: Correlation between FLT3-ITD status and clinical, cellular and molecular profiles in promyelocytic acute leukemias.
abstract: Internal tandem duplications (ITD) of FLT3 gene occur in about a third of acute promyelocytic leukemias (APL). We investigated the patterns of blood count, surface antigen, expression, chromosome aberrations, PML-RARa isoform, gene expression profile (GEP) and survival in 34 APL patients according to FLT3-ITD status. 97% had a t(15;17) and all of them carried PML-RARa gene fusion, 8 (23.5%) had a FLT3-ITD mutation. Presence of ITD was associated with higher Hb and WBC levels, bcr3 isoform, CD34 expression, CD2 or CD2/CD34 expression. In a multivariate analysis, Hb>9.6g/dL and WBC≥20 × 10(9)/L were important factors for predicting ITD presence. GEP showed that FLT3-ITD carriers clustered separately, even when as few as 5 genes were considered. This study provides further evidence that FLT3-ITDs carriers constitute a biologically distinct group of APL patients.
authors.affiliation: Laboratório BIOCOD Biotecnologia Ltda., Av. das Nações 2448, Portaria A, Vespasiano CEP 33200-000, MG, Brazil. Electronic address: inacatrina@yahoo.com.br.
authors.forename: Carolina Pereira
authors.initials: CP
authors.lastname: Souza Melo
authors.affiliation: Laboratório BIOCOD Biotecnologia Ltda., Av. das Nações 2448, Portaria A, Vespasiano CEP 33200-000, MG, Brazil. Electronic address: catharina.campos@labhpardini.com.br.
authors.forename: Catharina Brant
authors.initials: CB
authors.lastname: Campos
authors.affiliation: Hospital Santa Casa de Misericórdia de Belo Horizonte, Av. Francisco Sales 1111, Belo Horizonte CEP 30150-221, MG, Brazil. Electronic address: aloapd@hotmail.com.
authors.forename: Álvaro Pimenta
authors.initials: ÁP
authors.lastname: Dutra
authors.affiliation: Hospital Santa Casa de Misericórdia de Belo Horizonte, Av. Francisco Sales 1111, Belo Horizonte CEP 30150-221, MG, Brazil. Electronic address: caetanoaguirre@hotmail.com.
authors.forename: Joaquim Caetano Aguirre
authors.initials: JC
authors.lastname: Neto
authors.affiliation: Hospital Santa Casa de Misericórdia de Belo Horizonte, Av. Francisco Sales 1111, Belo Horizonte CEP 30150-221, MG, Brazil. Electronic address: afenelon@terra.com.br.
authors.forename: Alexandre José Silva
authors.initials: AJ
authors.lastname: Fenelon
authors.affiliation: Hospital Universitário da Universidade Federal de Juiz de Fora, Rua Catulo Breviglieri s/n, Juiz de Fora CEP 36036-110, MG, Brazil. Electronic address: abrahallack@ig.com.br.
authors.forename: Abrahão Hallack
authors.initials: AH
authors.lastname: Neto
authors.affiliation: Hospital Pequeno Príncipe, Rua Desembargador Motta 1070, Curitiba CEP 80250-060, PR, Brazil. Electronic address: ednacarboni@bol.com.br.
authors.forename: Edna Kakitani
authors.initials: EK
authors.lastname: Carbone
authors.affiliation: Hospital Erasto Gaertner, Rua Dr. Ovande do Amaral 201, Curitiba CEP 81520-060, PR, Brazil. Electronic address: albonei@yahoo.com.br.
authors.forename: Mara Albonei Dudeque
authors.initials: MA
authors.lastname: Pianovski
authors.affiliation: Setor de Pesquisa e Desenvolvimento-Instituto Hermes Pardini, Av. das Nações 2448, Portaria A, Vespasiano CEP 33200-000, MG, Brazil. Electronic address: alessandro.ferreira@labhpardini.com.br.
authors.forename: Alessandro Clayton de Souza
authors.initials: AC
authors.lastname: Ferreira
authors.affiliation: Laboratório BIOCOD Biotecnologia Ltda., Av. das Nações 2448, Portaria A, Vespasiano CEP 33200-000, MG, Brazil; Setor de Pesquisa e Desenvolvimento-Instituto Hermes Pardini, Av. das Nações 2448, Portaria A, Vespasiano CEP 33200-000, MG, Brazil. Electronic address: julianaassumpcao@yahoo.com.br.
authors.forename: Juliana Godoy
authors.initials: JG
authors.lastname: Assumpcão
mesh_terms: D018952:Antigens, CD34 / Q000096:biosynthesis / Q000235:genetics; D018801:CD2 Antigens / Q000096:biosynthesis / Q000235:genetics; D002884:Chromosomes, Human, Pair 15 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D014178:Translocation, Genetic; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics* / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D018801:CD2 Antigens; D015514:Oncogene Proteins, Fusion; C093476:promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein; C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3
keywords: 17); AML M3; Acute promyelocytic leukemia; FLT3–ITD; PML–RARA; t(15