title: Biocompatibility, cell growth and clinical relevance of synthetic meshes and biological matrixes for internal support in implant-based breast reconstruction.
abstract: OBJECTIVE
  Biological matrixes and synthetic meshes are increasingly used in implant-based breast reconstruction (IBBR). The objective was to test different materials used for internal support in IBBR in regards to biocompatibility and discuss possible limitations in a clinical context.
  
  
  METHODS
  In vitro investigations were performed on four relevant cell lines: Normal Human Dermal Fibroblasts (NHDF), Human White Preadipocytes (HWP), Endothelial cells (HDMEC) and Skeletal muscle cells (SkMC). A titanium-coated polypropylene mesh (TiLOOP Bra), a partially resorbable mesh (SERAGYN BR) and a porcine derived biologic matrix (Strattice) were investigated. Test of cytotoxicity, cell proliferation and oxidative stress was performed. Real-time cell analysis was used to determine adhesion rate. Light- and scanning electron microscopy investigated cell migration.
  
  
  RESULTS
  No relevant cytotoxicity was detected for any mesh or matrix. Good cell proliferation was observed in all materials with best results for NHDF and SkMC. For HWP and HDMEC decreased proliferation and adherence to the synthetic meshes and biologic matrix were observed. Real-time cell analysis of fibroblasts incubated with the corresponding material, showed increased impedance for the synthetic meshes. A morphologic cell change was observed within all materials. Scanning electron microscopy showed good cell penetration into the meshes and matrix. The material compositions did not seem to influence the clinical outcome, although the biological matrix was much thicker compared to the synthetic meshes.
  
  
  CONCLUSIONS
  Biochemical examination showed good biocompatibility for the investigated meshes and matrix. All products seem to have their value in IBBR and can be recommended for IBBR.
authors.affiliation: Breast Unit, Department of Obstetrics and Gynecology, Interdisciplinary Breast Center, University of Rostock, Südring 81, 18059, Rostock, Germany, max.dieterich@uni-rostock.de.
authors.forename: Max
authors.initials: M
authors.lastname: Dieterich
authors.forename: Johannes
authors.initials: J
authors.lastname: Stubert
authors.forename: Bernd
authors.initials: B
authors.lastname: Gerber
authors.forename: Toralf
authors.initials: T
authors.lastname: Reimer
authors.forename: Dagmar-Ulrike
authors.initials: DU
authors.lastname: Richter
mesh_terms: D000818:Animals; D019928:Breast Implantation / Q000379:methods*; D018427:Breast Implants; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D016462:Mammaplasty / Q000295:instrumentation / Q000379:methods*; D011126:Polypropylenes / Q000737:chemistry; D013526:Surgical Mesh; D013552:Swine; D000267:Tissue Adhesions; D014025:Titanium / Q000737:chemistry
chemical_list: D011126:Polypropylenes; C568444:strattice; D003094:Collagen; D014025:Titanium