title: Differential protein expression in chicken spermatozoa before and after freezing-thawing treatment.
abstract: The biological characteristics of rooster sperm that has undergone freezing treatment remain elusive. This study analyzed the change in sperm proteins after freezing-thawing treatment by using a proteomic approach. Semen from three 36-wk-old L2 strain Taiwan country chickens were used. A qualifying ejaculate containing more than 80% motility and volume 200μL was used for cryopreservation. The proteomic analysis explored 55 protein spots that differed significantly before and after freezing-thawing treatment (P<0.05). Among the 55 protein spots, expression levels of 19 proteins decreased after treatment. Forty-five differentially expressed protein spots were identified and belong to 33 proteins. Results of gene ontology analysis revealed that most differentially expressed proteins were involved in molecular function of the cellular metabolism process (28%) and cellular carbohydrate metabolism process (15%), and were associated with molecular function of oxidoreductase activity (19%) and protein binding (18%). The differentially expressed proteins before and after freezing-thawing treatment, including fructose-bisphosphate aldolase C, triosephosphate isomerase, aconitate hydratase, tubulin and outer dense-fiber protein, are associated with sperm energy metabolism and flagellum structure. In conclusion, freezing-thawing treatment significantly affects the expression of proteins related to sperm metabolism and structure in chicken spermatozoa. The differing levels of these proteins could be valuable for further enhancing the fertility of frozen-thawed chicken spermatozoa.
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Chuen-Yu
authors.initials: CY
authors.lastname: Cheng
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Pin-Rong
authors.initials: PR
authors.lastname: Chen
authors.affiliation: Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan; Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan.
authors.forename: Chao-Jung
authors.initials: CJ
authors.lastname: Chen
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Shin-Han
authors.initials: SH
authors.lastname: Wang
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Chih-Feng
authors.initials: CF
authors.lastname: Chen
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Yen-Pai
authors.initials: YP
authors.lastname: Lee
authors.affiliation: Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan; Center for the Integrative and Evolutionary Galliformes Genomics, iEGG Center, National Chung Hsing University, Taichung, 40227, Taiwan; Center of Nanoscience and Nanotechnology, National Chung Hsing University, Taichung, 40227, Taiwan. Electronic address: syhuang@dragon.nchu.edu.tw.
authors.forename: San-Yuan
authors.initials: SY
authors.lastname: Huang
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary*; D005615:Freezing; D005786:Gene Expression Regulation / Q000502:physiology*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013094:Spermatozoa / Q000378:metabolism*
keywords: Chicken; Cryopreservation; Proteome; Sperm