title: [Enhanced expression of EPHX2 gene in the kidney of the hypertensive ISIAH rats].
abstract: Epoxyeicosatrienoic acids (EETs) have antihypertensive properties and play a part in the maintenance of renal microvascular function. EETs mediate vasodilation of rat preglomerular microvessels and activate ion channels. Ephx2 is coding for the soluble epoxide hydrolase (sEH) which catalyze the degradation of EETs. Renal cortex and renal medulla were tested for Ephx2 mRNA level in hypertensive ISIAH and normotensive WAG rats at rest and emotional stress conditions. The microarray analysis and real-time PCR were used to assess the transcriptional activity of Ephx2. Enhanced transcriptional activity of Ephx2 in both renal structures of ISIAH rats was found at rest and stress conditions. The emotional stress caused elevation of Ephx2 mRNA level in renal medulla of ISIAH rats and opposite response--a decrease in Ephx2 expression in the renal medulla and cortex of WAG rats.The results suggest Ephx2 participation in the control of the vascular tone changes in kidney promoting the hypertensive state in the ISIAH rats.
authors.forename: T O
authors.initials: TO
authors.lastname: Abramova
authors.forename: O E
authors.initials: OE
authors.lastname: Redina
authors.forename: S E
authors.initials: SE
authors.lastname: Smolenskaia
authors.forename: A L
authors.initials: AL
authors.lastname: Markel
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000235:genetics / Q000378:metabolism*; D001794:Blood Pressure / Q000235:genetics; D004851:Epoxide Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D006977:Hypertension, Renal / Q000235:genetics* / Q000473:pathology; D008297:Male; D051381:Rats; D013315:Stress, Psychological; D014664:Vasodilation / Q000235:genetics
chemical_list: D001095:Arachidonic Acids; D004851:Epoxide Hydrolases; C529022:EPHX2 protein, rat