title: Study of differential properties of fibrochondrocytes and hyaline chondrocytes in growing rabbits.
abstract: We aimed to build a culture model of chondrocytes in vitro, and to study the differential properties between fibrochondrocytes and hyaline chondrocytes. Histological sections were stained with haematoxylin and eosin so that we could analyse the histological structure of the fibrocartilage and hyaline cartilage. Condylar fibrochondrocytes and femoral hyaline chondrocytes were cultured from four, 4-week-old, New Zealand white rabbits. The production of COL2A1, COL1OA1, SOX9 and aggrecan was detected by real time-q polymerase chain reaction (RT-qPCR) and immunoblotting and the differences between them were compared statistically. Histological structures obviously differed between fibrocartilage and hyaline cartilage. COL2A1 and SOX9 were highly expressed within cell passage 2 (P2) of both fibrochondrocytes and hyaline chondrocytes, and reduced significantly after cell passage 4 (P4). The mRNA expressions of COL2A1 (p=0.05), COL10A1 (p=0.04), SOX9 (p=0.03), and aggrecan (p=0.04) were significantly higher in hyaline chondrocytes than in fibrochondrocytes, whereas the expression of COL1A1 (p=0.02) was the opposite. Immunoblotting showed similar results. We have built a simple and effective culture model of chondrocytes in vitro, and the P2 of chondrocytes is recommended for further studies. Condylar fibrocartilage and femoral hyaline cartilage have unique biological properties, and the regulatory mechanisms of endochondral ossification for the condyle should be studied independently in the future.
authors.affiliation: Department of Oral & Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Electronic address: dshuanglinjian@126.com.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Fudan University School of Medicine, Shanghai 200032, China. Electronic address: ranisme@163.com.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Oral & Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Electronic address: lihui9x@163.com.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Oral & Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Electronic address: yangchi63@hotmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Oral & Maxillofacial Surgery, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai 200011, China. Electronic address: caixieyi27@126.com.
authors.forename: X
authors.initials: X
authors.lastname: Cai
mesh_terms: D053674:Aggrecans / Q000032:analysis; D000818:Animals; D002358:Cartilage, Articular / Q000737:chemistry / Q000166:cytology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000737:chemistry / Q000502:physiology*; D024042:Collagen Type I / Q000032:analysis; D024043:Collagen Type II / Q000032:analysis; D024121:Collagen Type X / Q000032:analysis; D005260:Female; D005269:Femur / Q000737:chemistry / Q000166:cytology; D051445:Fibrocartilage / Q000737:chemistry / Q000166:cytology*; D051457:Hyaline Cartilage / Q000737:chemistry / Q000166:cytology*; D006984:Hypertrophy; D008335:Mandibular Condyle / Q000737:chemistry / Q000166:cytology; D011817:Rabbits; D055758:SOX9 Transcription Factor / Q000032:analysis
chemical_list: D053674:Aggrecans; D024042:Collagen Type I; D024043:Collagen Type II; D024121:Collagen Type X; D055758:SOX9 Transcription Factor
keywords: Biological properties; Cartilage; Fibrochondrocytes; Hyaline chondrocytes