title: Trajectory-based co-localization measures for nanoparticle-cell interaction studies.
abstract: High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology - University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Juan A
authors.initials: JA
authors.lastname: Varela
authors.forename: Christoffer
authors.initials: C
authors.lastname: Åberg
authors.forename: Jeremy C
authors.initials: JC
authors.lastname: Simpson
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D021621:Imaging, Three-Dimensional; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D008247:Lysosomes / Q000737:chemistry; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry; D013269:Stochastic Processes; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes; D011137:Polystyrenes
keywords: bionanotechnology; co-localization; microscopy; nanoparticles