title: Curcumin attenuated acute Propionibacterium acnes-induced liver injury through inhibition of HMGB1 expression in mice.
abstract: Curcumin is a phenolic product isolated from the rhizome of Curcuma longa and has protective effects on inflammatory diseases. Here we investigated the protective effect of curcumin in acute Propionibacterium acnes (P. acnes)-induced inflammatory liver injury. C57BL/6 mice were primed with P. acnes followed by LPS challenge to induce fulminant hepatitis. Curcumin or vehicle control was administered perorally by gavage once daily starting 2days before P. acnes priming. We found that curcumin significantly improved mouse mortality. Then, to investigate the underlying mechanisms of curcumin in this acute inflammatory liver injury model, we primed C57BL/6 mice with P. acnes only. We found that curcumin treatment attenuated P. acnes-induced liver injury as evidenced by decreased production of ALT. In addition, curcumin treatment reduced the production of proinflammatory cytokines such as TNF-α and IFN-γ, accompanied by reduced hepatocyte apoptosis. Furthermore, curcumin treatment significantly reduced HMGB1 cytoplasmic translocation and expression by down-regulating acetylation of lysine. Taken together, our results suggest that curcumin protects mice from P. acnes-induced liver injury through reduction of HMGB1 cytoplasmic translocation and expression.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, Jiangsu, China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, Jiangsu, China.
authors.forename: Honggeng
authors.initials: H
authors.lastname: Guan
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, Jiangsu, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Shanghai Institute of Immunology and Institute of Health Sciences, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai Institutes for Biological Sciences, Shanghai, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Soochow University, 188 Shizi Road, Suzhou, Jiangsu, China. Electronic address: suzhouspine@163.com.
authors.forename: Huilin
authors.initials: H
authors.lastname: Yang
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D030024:Curcuma / Q000276:immunology; D003474:Curcumin / Q000008:administration & dosage* / Q000009:adverse effects; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000276:immunology; D024243:HMGB1 Protein / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000276:immunology; D007371:Interferon-gamma / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D017114:Liver Failure, Acute / Q000188:drug therapy* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011425:Propionibacterium acnes / Q000276:immunology*; D021381:Protein Transport / Q000187:drug effects; D027343:Rhizome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D024243:HMGB1 Protein; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D003474:Curcumin
keywords: Curcumin; HMGB1; Liver injury; Propionibacterium acnes