title: Assessment of the stability of proanthocyanidins and other phenolic compounds in cranberry syrup after gamma-irradiation treatment and during storage.
abstract: Shelf life of commercial cranberry syrup irradiated with gamma radiation at a rate of 5 kGy and stored for 6 months at 25 °C and 60% relative humidity (RH) and under accelerated stability conditions was investigated. High-performance liquid chromatography coupled to electrospray ionisation quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS) was used to characterise cranberry syrup. Afterwards, these compounds were quantified by HPLC-ESI-QTOF-MS and 4-dimethylaminocinnamaldehyde (DMAC) assay. A significant increase in the content of procyanidin B isomer 1 (from 4.4 to 7.0 μg/ml) and procyanidin A2 (from 83 to 93 μg/ml) was observed after irradiation and compared with the non-irradiated syrup. Procyanidin B isomers and prodelphinidin were stable at 25 °C during the first month of storage, whereas quercetin and some derivatives remained constant for 3 months of storage at this temperature. In short, after gamma-irradiation in dose of 5 kGy, most compounds were highly stable for a month at 25 °C.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento s/n, Edificio BioRegión, 18017 Granada, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Rodríguez-Pérez
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento s/n, Edificio BioRegión, 18017 Granada, Spain. Electronic address: rquirantes@ugr.es.
authors.forename: R
authors.initials: R
authors.lastname: Quirantes-Piné
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento s/n, Edificio BioRegión, 18017 Granada, Spain.
authors.forename: M del M
authors.initials: Mdel M
authors.lastname: Contreras
authors.affiliation: UGC Pediatría, Hospital Clínico San Cecilio Granada, 18012 Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Uberos
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento s/n, Edificio BioRegión, 18017 Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Gutiérrez
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain; Research and Development Functional Food Centre (CIDAF), Health Science Technological Park, Avenida del Conocimiento s/n, Edificio BioRegión, 18017 Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Segura-Carretero
mesh_terms: D044946:Biflavonoids / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis; D004307:Dose-Response Relationship, Radiation; D005514:Food Irradiation; D061353:Food Storage; D005720:Gamma Rays; D057230:Limit of Detection; D010636:Phenols / Q000032:analysis*; D044945:Proanthocyanidins / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D029799:Vaccinium macrocarpon / Q000737:chemistry*
chemical_list: D044946:Biflavonoids; D002934:Cinnamates; D010636:Phenols; D044945:Proanthocyanidins; C542735:procyanidin A2; C479578:procyanidin B; C013221:proanthocyanidin; D002392:Catechin; C018523:4-dimethylaminocinnamaldehyde
keywords: Cranberry; DMAC; Gamma-irradiation; HPLC; Mass spectrometry; Stability