title: An analysis of 72% chromated glycerin used for sclerotherapy: sterility, potency, and cost after extended shelf life.
abstract: BACKGROUND
  Sclerotherapy is the treatment of reticular veins and telangiectasias of the lower extremities. Sclerosants destroy endothelial tissue and expose subendothelial collagen fibers, which lead to subsequent fibrosis of vessels, thus preventing recanalization. There are several available sclerosants including sodium tetradecyl sulfate (STS), polidocanol (POL), and chromated glycerin (CG) with varying efficacy, potency, side effect profile, and cost.
  
  
  OBJECTIVE
  To identify the possible bacterial contamination and potency of CG beyond the current recommended shelf life of 3 months and to prove if CG is as cost effective as other available sclerosants.
  
  
  METHODS
  Samples of 72% CG underwent bacterial endotoxin, sterility, and potency analysis at Days 0, 24, and 183. In addition, cost comparison was performed with other commercially available sclerosants including STS and POL.
  
  
  RESULTS
  No samples of CG showed any bacterial contamination. All aliquots of glycerin remained sterile at Day 14. Potency at Day 24 was 99.2%, which was the same at Day 183. Cost comparison with other sclerosants revealed that CG is lower cost per milliliter than STS and POL.
  
  
  CONCLUSIONS
  Seventy-two percent CG has no contamination and maintains its reported potency up to 6 months while comparable with the cost of other commercially available sclerosants.
authors.affiliation: *Division of Plastic and Reconstructive Surgery, Department of Surgery, Henry Ford Health System, Detroit, Michigan; †Wayne State University School of Medicine, Detroit, Michigan.
authors.forename: Amir M
authors.initials: AM
authors.lastname: Ghaznavi
authors.forename: Mio
authors.initials: M
authors.lastname: Nakamura
authors.forename: Donna
authors.initials: D
authors.lastname: Tepper
mesh_terms: D002840:Chromates / Q000737:chemistry* / Q000191:economics; D015169:Colony Count, Microbial; D003365:Costs and Cost Analysis; D004356:Drug Storage; D004731:Endotoxins / Q000032:analysis; D005990:Glycerol / Q000737:chemistry* / Q000191:economics; D000077423:Polidocanol; D011092:Polyethylene Glycols / Q000191:economics; D012597:Sclerosing Solutions / Q000737:chemistry* / Q000191:economics; D015911:Sclerotherapy / Q000191:economics; D012981:Sodium Tetradecyl Sulfate / Q000191:economics; D013242:Sterilization; D013997:Time Factors
chemical_list: D002840:Chromates; D004731:Endotoxins; D012597:Sclerosing Solutions; D000077423:Polidocanol; D011092:Polyethylene Glycols; D005990:Glycerol; D012981:Sodium Tetradecyl Sulfate