title: Detection of the causal agents of Keriorrhea (Lepidocybium flavobrunneum and Ruvettus pretiosus) by means of Real Time PCR.
abstract: Consumption of Lepidocybium flavobrunneum and Ruvettus pretiosus is related with the gastrointestinal disease called Keriorrhea. Sometimes, intentionally or not, these species are mislabelled as other harmless species, causing severe disruptions to consumers. The correct identification of these species helps to avoid food fraud and health problems. For this reason, a multiplex Real Time-PCR method based on TaqMan technology for the correct authentication of L. flavobrunneum and R. pretiosus has been developed. The method is based on a species-specific set of primers and TaqMan probe which amplifies a 276 bp fragment of the cytochrome oxidase I (COI) mitochondrial DNA region. This methodology allows your application to any type of product, regardless of the degree of processing it has undergone with high specificity, sensitivity and rapidity. Also, it might be a useful tool in monitoring and verifying labelling regulation and protect consumer rights.
authors.affiliation: Research Department of Living Resources, Aquaculture and Its Traceability Management, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain.
authors.forename: Jacobo
authors.initials: J
authors.lastname: Dalama
authors.affiliation: Research Department of Living Resources, Aquaculture and Its Traceability Management, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain.
authors.forename: Juan M
authors.initials: JM
authors.lastname: Vieites
authors.affiliation: Research Department of Living Resources, Aquaculture and Its Traceability Management, ANFACO-CECOPESCA, Vigo 36310, Pontevedra, Spain. Electronic address: montse@anfaco.es.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Espiñeira
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000209:etiology*; D029941:Fish Proteins / Q000032:analysis; D005399:Fishes / Q000145:classification / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D029941:Fish Proteins
keywords: COI; Detection; Keriorrhea; Lepidocybium flavobrunneum; Real-Time PCR; Ruvettus pretiosus; Species authentication; Toxic fish