title: Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum.
abstract: Extraction was optimized of polysaccharides from Gleoestereum incarnatum (GIP). The three parameters, extraction temperature, extraction time and the ratio of water to raw material, were optimized using the Box-Behnken design. As a result, the optimal extraction conditions were: extraction temperature 87.5 °C, extraction time 1 h and the ratio of water to raw material of 39.7 mL/g, where the highest yield of polysaccharide (13.18%) was obtained. GIP-II was the main fraction purified form GIP. GIP-II was composed of galactose, glucose, xylose, and mannose, with glucose was the predominant monosaccharide. GIP-II exhibited strong scavenging activities against DPPH and hydroxyl radials in vitro, as well as a strong inhibitory effect on the growth of HepG2 cells. The overall findings indicated that GIP-II is worthy of further exploration for its potential applications in antitumor drugs or health foods.
authors.affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China. Electronic address: zzf2050@sohu.com.
authors.forename: Zuo-Fa
authors.initials: ZF
authors.lastname: Zhang
authors.affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Lv
authors.affiliation: College of Animal Science, Zhejiang University, Hangzhou 310029, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Cheng
authors.affiliation: Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China.
authors.forename: Lei-Fa
authors.initials: LF
authors.lastname: Fan
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant activity; Antitumor activity; Gleoestereum incarnatum; Polysaccharide