title: MiR-378a-3p enhances adipogenesis by targeting mitogen-activated protein kinase 1.
abstract: Previous studies showed that miR-378a plays important roles in adipogenesis and obesity; however, the precise mechanisms of action remain unknown. Here, we found that miR-378a-3p expression is up-regulated in adipose tissues of high fat diet-induced obese mice, as well as during the differentiation of 3T3-L1 preadipocytes. Mir-378a-3p induced adipogenesis by targeting mitogen-activated protein kinase 1 (MAPK1). Overexpression of miR-378a-3p or silencing MAPK1 reduced MAPK1 expression and enhanced adipogenesis, whereas blockage of endogenous miR-378a-3p had the opposite effect, suggesting that miR-378a-3p promotes the adipogenesis of 3T3-L1 cells by targeting MAPK1.
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, PR China; Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Nunu
authors.initials: N
authors.lastname: Huang
authors.affiliation: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Jiu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Xie
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, PR China; Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Lyu
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, PR China; Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Jiangbin
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: School of Life Sciences, Tsinghua University, Beijing 100084, PR China; Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China. Electronic address: xu.naihan@sz.tsinghua.edu.cn.
authors.forename: Naihan
authors.initials: N
authors.lastname: Xu
authors.affiliation: Key Lab in Healthy Science and Technology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, PR China. Electronic address: zhangyo@sz.tsinghua.edu.cn.
authors.forename: Yaou
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D059305:Diet, High-Fat; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D008954:Models, Biological; D008969:Molecular Sequence Data; D009765:Obesity / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation / Q000235:genetics
chemical_list: C546119:MIRN378 microRNA, mouse; D035683:MicroRNAs; D019950:Mitogen-Activated Protein Kinase 1
keywords: Adipogenesis; MAPK1; MiR-378a-3p