title: Alpha adrenergic receptors in renal pelvis and calyces: can rat models be used?
abstract: We aimed, in this study, to determine the distribution of α-1 AR subtypes in rat and human pelvis and calyces, and to evaluate, by comparing these two species, the possibility of rats to be used as models for humans. Twenty patients with renal carcinoma were included into the study. The patients underwent radical nephrectomy for renal cell carcinoma (RCC). After nephrectomy, specimens were evaluated and excisional biopsies from healthy pelvis and calyces tissues were performed. When pathology confirmed the non-invasion of RCC, specimen was included into the study. A total of 7 adult Wistar Albino (250-300 g) female rats were used in this study. Specimens included renal pelvis and calyces. All specimens were evaluated under light microscope histopathologically. The concentrations of the receptor densities did not differ between the two groups. With the demonstration of the α receptors in rat kidneys and calyces, many receptor-based studies concerning both humans and rats can take place. Novel medication targeting these subtypes -in this matter α1A and α1D for renal pelvis and calyces- may be helpful for expulsive therapy and/or pain relief. With the demonstration of similar receptor densities between human and rat tissues, rat model may be useful for α-receptor trials for renal pelvis and calyces.
authors.affiliation: Ministry of Health, Ankara Diskapi Yildirim Beyazit Teaching and Research Hospital, Department of Urology, Turkey.
authors.forename: Osman Raif
authors.initials: OR
authors.lastname: Karabacak
authors.affiliation: Department of Urology, Ministry of Health, Adana Numune Teaching and Research Hospital, Turkey.
authors.forename: Nevzat Can
authors.initials: NC
authors.lastname: Sener
authors.affiliation: Department of Pathology, Ministry of Health, Ankara Diskapi Yildirim Beyazit Teaching and Research Hospital, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Yilmazer
authors.affiliation: Department of Women's Health, Ministry of Health, Ankara, Turkey.
authors.forename: Yurdum
authors.initials: Y
authors.lastname: Karabacak
authors.affiliation: Ministry of Health, Ankara Diskapi Yildirim Beyazit Teaching and Research Hospital, Department of Urology; Turkey.
authors.forename: Hasan Nedim Goksel
authors.initials: HN
authors.lastname: Goktug
authors.affiliation: Ministry of Health, Ankara Diskapi Yildirim Beyazit Teaching and Research Hospital, Department of Urology.
authors.forename: Orhan
authors.initials: O
authors.lastname: Yigitbasi
authors.affiliation: Ministry of Health, Ankara Diskapi Yildirim Beyazit Teaching and Research Hospital, Department of Pathology.
authors.forename: Murat
authors.initials: M
authors.lastname: Alper
mesh_terms: D000818:Animals; D001706:Biopsy; D002292:Carcinoma, Renal Cell / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007670:Kidney Calices / Q000737:chemistry*; D007680:Kidney Neoplasms / Q000737:chemistry; D007682:Kidney Pelvis / Q000737:chemistry*; D023421:Models, Animal; D009392:Nephrectomy; D017208:Rats, Wistar; D011942:Receptors, Adrenergic, alpha / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D011942:Receptors, Adrenergic, alpha