title: Identification and characterization of a novel splice variant of the PLCζ1 gene in bull testis tissues.
abstract: Phospholipase C zeta 1 (PLCζ1), which transcribes a key protein, has an important function in oocyte activation and embryo development because PLCζ1 can trigger a series of intracellular Ca2+ oscillations in mammals. In this study, a novel splice variant in the testis tissues of adult and fetal Chinese Holstein bulls was characterized by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing analysis. The novel splice variant PLCζ1-sv1 was derived from the PLCζ1 complete transcript (PLCζ1-complete) by alternative splicing; the alternative splicing pattern exhibited alternative 5'-splice sites. The full-length transcript, PLCζ1-complete, is the main transcript found in fetal and adult cow testis tissue. Quantitative real-time PCR (qPCR) analysis demonstrated that the expression levels of the PLCζ1-complete transcript were significantly higher than those of the PLCζ1-sv1 splice variant in bovine testis tissues. PLCζ1 protein sequencing analysis showed that the amino acids at positions 453 to 457 were deleted in PLCζ1-sv1, thereby terminating transcription prematurely. In summary, this study provided information to elucidate the structure and function of the bovine PLCζ1 gene.
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Ju
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Huang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: C
authors.initials: C
authors.lastname: Qi
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Wang
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: Q L
authors.initials: QL
authors.lastname: Li
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhong
authors.affiliation: College of Life Science, Nanjing Normal University, Nanjing, China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.affiliation: Dairy Cattle Research Center, Shandong Academy of Agricultural Science, Jinan, China wangcf1967@163.com.
authors.forename: C F
authors.initials: CF
authors.lastname: Wang
mesh_terms: D017398:Alternative Splicing / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002681:China; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005091:Exons / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D059372:Nucleotide Motifs / Q000235:genetics; D051966:Phospholipase C gamma / Q000737:chemistry / Q000235:genetics*; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000201:enzymology*; D014158:Transcription, Genetic
chemical_list: D012333:RNA, Messenger; D051966:Phospholipase C gamma