title: Development of a new immunochromatographic assay using gold nanoparticles for screening of IgA deficiency.
abstract: A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay. In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL. The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.
authors.affiliation: Clinical Nanomedicine Laboratory, ENT- Head & Neck Research Center, Hazrate Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. goudarzi@behdasht.gov.ir.
authors.forename: Saeid
authors.initials: S
authors.lastname: Goudarzi
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, and Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. A_ahmadi_aa@yahoo.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Ahmadi
authors.affiliation: Clinical Nanomedicine Laboratory, ENT- Head & Neck Research Center, Hazrate Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. Farhadi@ent-hns.org.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farhadi
authors.affiliation: Clinical Nanomedicine Laboratory, ENT- Head & Neck Research Center, Hazrate Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. skkamrava@yahoo.com.
authors.forename: Seyed Kamran
authors.initials: SK
authors.lastname: Kamrava
authors.affiliation: Immunology, Asthma & Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran. shiva_saghafi@yahoo.com.
authors.forename: Shiva
authors.initials: S
authors.lastname: Saghafi
authors.affiliation: Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, and Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran. omidfar@sina.tums.ac.ir.
authors.forename: Kobra
authors.initials: K
authors.lastname: Omidfar
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D006046:Gold; D006801:Humans; D017098:IgA Deficiency / Q000175:diagnosis*; D007070:Immunoglobulin A / Q000032:analysis*; D053768:Metal Nanoparticles; D015203:Reproducibility of Results
chemical_list: D007070:Immunoglobulin A; D006046:Gold