title: Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury.
abstract: OBJECTIVE
  To investigate the effects of acute hyperglycemia on dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury.
  
  
  METHODS
  Sprague-Dawley rats were randomly arranged to the normoglycemic (NG) or hyperglycemic group (HG), with each group further divided into sham (no I/R injury), I/R (ischemia-reperfusion) and dex (given by dexmedetomidine) groups. Acute hyperglycemia was induced by intraperitoneal injection (i.p.) of 25% glucose (3 g/kg) 45 min before ischemia. Dexmedetomidine (50 μg/kg, i.p.) was administrated 30 min before induction of ischemia. Renal function, histology, apoptosis, expression of Bax, Bcl-2 and phosphorylated AKT (p-AKT) were detected.
  
  
  RESULTS
  I/R insult significantly increased the serum levels of blood urea nitrogen and creatinine, apoptotic tubular epithelial cells, expression of Bax and p-AKT, but decreased Bcl-2 expression. All these changes were further enhanced by hyperglycemia (p<0.05). In hyperglycemic condition, there was no statistically difference between the I/R group and Dex group in all the aforementioned detection indexes (p>0.05).
  
  
  CONCLUSIONS
  Acute hyperglycemia attenuates dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury in non-diabetic rats.
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, Hubei, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, Hubei, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University, Wuhan, China.
authors.forename: Xiuheng
authors.initials: X
authors.lastname: Liu
mesh_terms: D000208:Acute Disease; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001786:Blood Glucose; D003404:Creatinine / Q000097:blood; D020927:Dexmedetomidine / Q000494:pharmacology*; D006943:Hyperglycemia / Q000139:chemically induced / Q000503:physiopathology*; D007511:Ischemia / Q000139:chemically induced* / Q000188:drug therapy; D019194:Ischemic Preconditioning; D007668:Kidney / Q000098:blood supply*; D007684:Kidney Tubules / Q000187:drug effects / Q000473:pathology; D008297:Male; D023421:Models, Animal; D009392:Nephrectomy; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D014508:Urea / Q000097:blood
chemical_list: D001786:Blood Glucose; D020927:Dexmedetomidine; D014508:Urea; D003404:Creatinine; D051057:Proto-Oncogene Proteins c-akt