title: A single nucleotide polymorphism in the TGF-β1 gene (rs1982073 C>T) may contribute to increased risks of bone fracture, osteoporosis, and osteoarthritis: a meta-analysis.
abstract: Genetic factors have been shown to be of great importance for the pathogenesis of bone diseases, such as fracture, osteoporosis (OP), and osteoarthritis (OA). However, published studies on the correlations of transforming growth factor-β1 (TGF-β1) gene polymorphisms with bone diseases have been hampered by small sample sizes or inconclusive findings. We hence aimed at examining the relationships between a single nucleotide polymorphism in the TGF-β1 gene (rs1982073 C>T) with bone fracture, OP, and OA risks in this meta-analysis. A systematic electronic search of literature was conducted to identify all published studies in English or Chinese on the association between the TGF-β1 gene and fracture, OP, or OA risks. Data were abstracted independently by two reviewers. To investigate the strength of this relationship, crude odds ratios with 95 % confidence intervals were used. An updated meta-analysis based on nine independent case-control studies were chosen (patients with fracture, OP, or OA = 1569; healthy controls = 1638). Results identified a higher frequency of rs1982073 C>T in patients with fracture, OP, or OA than in healthy controls. Ethnicity and genotyping method-stratified analysis under both models implied that the rs1982073 C>T polymorphism was positively correlated with the risk of fracture, OP, and OA among Asians under detection via the non-PCR-RFLP method. Disease-stratified results yielded that rs1982073 C>T may increase the risk of fracture, OP, and OA under the allele model, but was only significantly related to OP under the dominant model. According to the sample size-stratified analysis, subjects with the rs1982073 C>T polymorphism in the allele model were more likely to develop the three bone diseases in both the small and large sample size groups, and only in the large sample size under the dominant model. Our findings show that TGF-β1 rs1982073 C>T has a modest effect in increasing susceptibility to bone fracture, OP, and OA.
authors.affiliation: Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, Zhongshan East Road, No. 305, Nanjing, 210002, People's Republic of China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Department of Orthopedics, Jiangsu Provincial Corps Hospital of the Chinese People' Armed Police Force, Yangzhou, 225003, People's Republic of China.
authors.forename: Jiang-Ying
authors.initials: JY
authors.lastname: Ru
authors.affiliation: Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, Zhongshan East Road, No. 305, Nanjing, 210002, People's Republic of China.
authors.forename: Ni-Rong
authors.initials: NR
authors.lastname: Bao
authors.affiliation: Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, Zhongshan East Road, No. 305, Nanjing, 210002, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, Jinling Hospital, School of Medicine, Nanjing University, Zhongshan East Road, No. 305, Nanjing, 210002, People's Republic of China. jianyuzhao702@163.com.
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D050723:Fractures, Bone / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010003:Osteoarthritis / Q000235:genetics*; D010024:Osteoporosis / Q000235:genetics*; D058866:Osteoporotic Fractures / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D012307:Risk Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1
keywords: Bone fracture; Meta-analysis; Osteoarthritis; Osteoporosis; Single nucleotide polymorphism; Transforming growth factor-β1