title: [Composition of the oil-slime microbial community determined by analysis of the 16S rRNA gene].
abstract: Analysis of the 16S rRNA genes of the cultured microorganisms of industrial oil-slime revealed predominance (-85-90%) of the Gammaproteobacteria in the community of aerobic heterotrophs and specific oil-slime degraders. Relation of the isolated strains with members of the genera Pseudomonas, Stenotrophomonas, and Enterobacter was established. Analysis of the same gene in the total DNA from the oil-slime revealed greater microbial diversity (-20 operative taxonomic units determined by T-RFLP) than in the cultured part of the community, which included -12 different colony types. Three major restriction fragments were found, with their total area -50%. These results demonstrated the low morphological and phylogenetic diversity of the oil-slime bacterial community.
authors.forename: T V
authors.initials: TV
authors.lastname: Grigor'eva
authors.forename: A V
authors.initials: AV
authors.lastname: Laĭkov
authors.forename: A A
authors.initials: AA
authors.lastname: Rizvanov
authors.forename: O N
authors.initials: ON
authors.lastname: Il'inskaia
authors.forename: R P
authors.initials: RP
authors.lastname: Naumova
mesh_terms: D020563:Gammaproteobacteria / Q000235:genetics / Q000254:growth & development; D059013:Microbial Consortia / Q000502:physiology*; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D010578:Petroleum; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S