title: [Effect of lentivirus-mediated hIL-24 gene on proliferation, migration and invasion of keloid fibroblasts].
abstract: OBJECTIVE
  To investigate the effect of hIL-24 gene on proliferation, migration and invasion activity of human keloid fibroblasts (KFs).
  
  
  METHODS
  hIL-24 gene was cloned into lentivirus vector, then the lentivirus particles expressing hlL-24 were infected into KF cells. Real-time PCR and Western blot were performed to examine the expression of hIL-24 in lentivirus infected cells. The growth ability was detected by MTT assay. The cell cycle was analyzed by flow cytometry, The invasion and migration were detected by matrigel invasion assay and wound healing assay.
  
  
  RESULTS
  Comparing to controls group and KF-NC group, the expression levels of hIL-24 mRNA and protein were both significantly up-regulated after 4 days of hIL-24 lentivims infection. Comparing with the KF-NC group, MTT assay showed that the A490 of KF-hlL-24 group was down-regulated after lentivims infection ( P < 0. 05 ). Comparing with the KF-NC group, Cell cycle test revealed hlL-24 gene could block KF cells in G1 [(75. 40 ±2. 10)% ] , the proportion of KF cells was decreased in S phase [(4. 96 ± 1. 60)% ] and G2 phase [(0.01 ± 0.01)% ]. After KF cells were infected(P <0.01). Transfection of hlL-24 lentivirus inhibited the migration and invasion activity of KF cells.
  
  
  CONCLUSIONS
  Lentivirus-mediated hlL-24 gene efficiently inhibits proliferation, cell cycle progression, migration and invasion activity of KF cells.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhiyuan
authors.forename: Shi
authors.initials: S
authors.lastname: Yucang
authors.forename: Liang
authors.initials: L
authors.lastname: Jie
authors.forename: Xu
authors.initials: X
authors.lastname: Xiaxing
authors.forename: Wu
authors.initials: W
authors.lastname: Zhixian
authors.forename: Li
authors.initials: L
authors.lastname: Ran
mesh_terms: D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology; D005822:Genetic Vectors; D006801:Humans; D007378:Interleukins / Q000235:genetics* / Q000502:physiology; D007627:Keloid / Q000235:genetics / Q000473:pathology*; D016086:Lentivirus; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection / Q000379:methods; D000099180:Interleukin-24
chemical_list: D007378:Interleukins; D012333:RNA, Messenger; D000099180:Interleukin-24