title: Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization.
abstract: A novel highly sensitive colorimetric assay for DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization was established. The DNA modified superparamagnetic beads were demonstrated to capture and enrich the target DNA in the hybridization buffer or human plasma. The hybridization chain reaction and enzyme-induced silver metallization on the gold nanoparticles were used as cascade signal amplification for the detection of target DNA. The metalization of silver on the gold nanoparticles induced a significant color change from red to yellow until black depending on the concentration of the target DNA, which could be recognized by naked eyes. This method showed a good specificity for the target DNA detection, with the capabilty to discriminate single-base-pair mismatched DNA mutation (single nucleotide polymorphism). Meanwhile, this approach exhibited an excellent anti-interference capability with the convenience of the magentic seperation and washing, which enabled its usage in complex biological systems such as human blood plasma. As an added benefit, the utilization of hybridization chain reaction and enzyme-induced metallization improved detection sensitivity down to 10pM, which is about 100-fold lower than that of traditional unamplified homogeneous assays.
authors.affiliation: Micro & Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Xu
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, and State Key Laboratory of Virology, Wuhan University, Wuhan, Hubei 430072, China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Zhang
authors.affiliation: Micro & Nano Integrated Biosystem (MINIBio) Laboratory, Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802, USA. Electronic address: sxz10@psu.edu.
authors.forename: Si-Yang
authors.initials: SY
authors.lastname: Zheng
mesh_terms: D000469:Alkaline Phosphatase; D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D021141:Nucleic Acid Amplification Techniques; D009693:Nucleic Acid Hybridization; D020641:Polymorphism, Single Nucleotide; D012834:Silver
chemical_list: D012834:Silver; D006046:Gold; D004247:DNA; D000469:Alkaline Phosphatase
keywords: Cascade amplification; DNA detection; Gold nanoparticles; Hybridization chain reaction; Metallization