title: Reducing X-Ray Induced Oxidative Damages in Fibroblasts with Graphene Oxide.
abstract: A major issue of X-ray radiation therapy is that normal cells can be damaged, limiting the amount of X-rays that can be safely delivered to a tumor. This paper describes a new method based on graphene oxide (GO) to protect normal cells from oxidative damage by removing free radicals generated by X-ray radiation using grapheme oxide (GO). A variety of techniques such as cytotoxicity, genotoxicity, oxidative assay, apoptosis, γ-H2AX expression, and micro-nucleus assay have been used to assess the protective effect of GO in cultured fibroblast cells. It is found that although GO at higher concentration (100 and 500 μg/mL) can cause cell death and DNA damage, it can effectively remove oxygen free radicals at a lower concentration of 10 μg/mL. The level of DNA damage and cell death is reduced by 48%, and 39%, respectively. Thus, low concentration GO can be used as an effective radio-protective agent in occupational and therapeutic settings.
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Peipei
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Chaoming
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Biomedical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Su
keywords: DNA damage; X-ray; graphene oxide; oxidative; reactive oxygen species (ROS)