title: Assessment of antiproliferative activity of pectic substances obtained by different extraction methods from rapeseed cake on cancer cell lines.
abstract: In this work the antiproliferative activity of pectic substances obtained by different extraction methods from defatted rapeseed cake was assessed on cancer cell lines. The process consisted of sequential treatment with alkalized water (pH∼8), EDTA (0.01 M), alkaline protease (Alkalase 2.4L) and a commercial pectinase preparation (Viscozyme L or Pectinex Ultra SP-L). Pectic extracts identification was performed using spectroscopy and chromatography techniques. FT-IR and HPLC-IR results suggest that the neutral pectic extracts produced would be arabinogalactans and β-galactans. All the pectic substances extracted (acid and neutral) from RSC exhibited antiproliferative activity, being more effective on MCF-7 cells than Caco-2. The most effective pectic extract was obtained by Alkalase 2.4 L which killed over 80% of MCF-7 cells and 60% of Caco-2 cells. At less than 10 mg/mL pectic extracts enriched in neutral sugars also exhibited antiproliferative activity (50 and 40%, respectively), which was superior to the modified citric pectins activity at the same concentration for the breast cancer cell line (61.6% for MCF-7 and 49.9% for Caco-2 cells). These results show that the antiproliferative activity depends on both the type of pectin (acid or neutral) and the extraction procedure.
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile.
authors.forename: M
authors.initials: M
authors.lastname: Cobs-Rosas
authors.affiliation: Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso, Chile. Electronic address: jacqueline.concha@uv.cl.
authors.forename: J
authors.initials: J
authors.lastname: Concha-Olmos
authors.affiliation: Escuela de Química y Farmacia, Facultad de Farmacia, Universidad de Valparaíso, Gran Bretaña 1093, Playa Ancha, Valparaíso, Chile; Centro Regional de Estudio en Alimentos Saludables, R12C1001, Av. Universidad 330, Curauma, Valparaíso, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Weinstein-Oppenheimer
authors.affiliation: Escuela de Ingeniería Bioquímica, Pontificia Universidad Católica de Valparaíso, General Cruz 34, Valparaíso, Chile; Centro Regional de Estudio en Alimentos Saludables, R12C1001, Av. Universidad 330, Curauma, Valparaíso, Chile.
authors.forename: M E
authors.initials: ME
authors.lastname: Zúñiga-Hansen
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D029741:Brassica rapa / Q000737:chemistry*; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation / Q000379:methods*; D006801:Humans; D007220:Industrial Waste; D061986:MCF-7 Cells; D010368:Pectins / Q000302:isolation & purification* / Q000494:pharmacology*; D058105:Polymerization
chemical_list: D000970:Antineoplastic Agents; D007220:Industrial Waste; D010368:Pectins
keywords: Antiproliferative activity; Caco-2; MCF-7; Pectinases; Rapeseed cake