title: A highly sensitive quartz crystal microbalance immunosensor based on magnetic bead-supported bienzymes catalyzed mass enhancement strategy.
abstract: A highly sensitive quartz crystal microbalance (QCM) immunosensor based on magnetic bead-supported bienzyme catalyzed mass enhanced strategy was developed for the detection of human immunoglobulin G (hIgG) protein. The high sensitive detection was achieved by increasing the deposited mass on the QCM crystal through the enhanced precipitation of 4-chloro-1-naphthol (CN) using higher amounts of horseradish peroxidase (HRP) and glucose oxidase (GOx) bienzymes attached on the magnetic beads (MB). The protein A (PA) and capture antibody (monoclonal anti-human IgG antibody produced in mouse, Ab1)-based QCM probe and the detection antibody (anti-human IgG antibody produced in goat, Ab2)-based MB/HRP/GOx bienzymatic bioconjugates were characterized using scanning electron microscope, transmission electron microscope, cyclic voltammetry, and electrochemical impedance spectroscopy techniques. Under the optimized experimental condition, the linear range and the detection limit of hIgG immunosensor were determined to be 5.0pg/mL-20.0ng/mL and 5.0±0.18pg/mL, respectively. The applicability of the present hIgG immunosensor was examined in hIgG spiked human serum samples and excellent recoveries of hIgG were obtained.
authors.affiliation: Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea.
authors.forename: Rashida
authors.initials: R
authors.lastname: Akter
authors.affiliation: Department of Chemistry, Chungnam National University, Daejeon 305-764, South Korea; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: ckrhee@cnu.ac.kr.
authors.forename: Choong Kyun
authors.initials: CK
authors.lastname: Rhee
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea. Electronic address: marahman@cnu.ac.kr.
authors.forename: Md Aminur
authors.initials: MA
authors.lastname: Rahman
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006041:Goats; D006735:Horseradish Peroxidase; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000648:ultrastructure; D051379:Mice; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D004800:Enzymes, Immobilized; D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase
keywords: Biocatalytic precipitation; Human Immunoglobulin; Immunosensor; Magnetic bead-supported bioconjugate; Protein detection; Quartz crystal microbalance