title: Development of photo-controllable hydrogen sulfide donor applicable in live cells.
abstract: Hydrogen sulfide (H2S) has multiple physiological roles, for example, in vasodilation and inflammation. It is a highly reactive gas under ambient conditions, so controllable H2S donors are required for studying its biological functions. Here, we describe the design, synthesis and application of a H2S donor (SPD-2) that utilizes xanthone photochemistry to control H2S release. H2S generation from SPD-2 was completely dependent on UVA-irradiation (325-385nm), as confirmed by methylene blue assay and by the use of a H2S-selective fluorescent probe. SPD-2 was confirmed to provide controlled H2S delivery in live cells, and should be suitable for various biological applications.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi 467-8603, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Fukushima
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi 467-8603, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Ieda
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi 467-8603, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Kawaguchi
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sasakura
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi 467-8603, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Miyata
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1, Tanabe-dori, Mizuho-ku, Nagoya-shi, Aichi 467-8603, Japan.
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D055808:Drug Discovery / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D006862:Hydrogen Sulfide / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D010777:Photochemistry / Q000379:methods*
chemical_list: D004337:Drug Carriers; D006862:Hydrogen Sulfide
keywords: Caged H(2)S; Hydrogen sulfide; Photolabile protecting group; Xanthone photocage