title: Increased serum ADAMTS-4 in knee osteoarthritis: a potential indicator for the diagnosis of osteoarthritis in early stages.
abstract: We compared serum levels of a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4, ADAMTS-5, matrix metalloproteinase (MMP)-1, and MMP-3 in patients with different stages of knee osteoarthritis (OA), and investigated the clinical significance of diagnosing OA in early stages. OA patients were divided into 2 groups: early OA group (44 cases), intermediate and advanced OA group (26 cases). The healthy control group included 30 samples. ADAMTS-4, ADAMTS-5, MMP-1, and MMP-3 levels in the serum were tested using an enzyme-linked immunosorbent assay. A protein-protein interaction network was constructed by seeding the significantly expressed marker, followed by Gene Ontology enrichment analyses using Database for Annotation, Visualization and Integrated Discovery. ADAMTS-4 levels were significantly higher in patients at early stages of OA compared to intermediate or advanced OA and healthy controls. ADAMTS-5, MMP-1, and MMP-3 levels in intermediate and advanced-stage OA patients were significantly higher than those in early-stage OA patients and healthy controls. The protein-protein interaction network showed that ADAMTS-4 participates in 67 interactions. Gene Ontology enrichment analysis validated that genes associated with ADAMTS-4 participate in collagen metabolism and OA. ADAMTS-4 is a potential biomarker as an early diagnostic indicator of OA.
authors.affiliation: Department of Nuclear Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Nuclear Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: C
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Nuclear Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Nuclear Medicine, Qianfoshan Hospital Affiliated to Shandong University, Jinan, China liwenqas@126.com.
authors.forename: C
authors.initials: C
authors.lastname: Zhang
mesh_terms: D051722:ADAM Proteins / Q000097:blood / Q000235:genetics*; D000071121:ADAMTS4 Protein; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003094:Collagen / Q000235:genetics* / Q000378:metabolism; D018450:Disease Progression; D042241:Early Diagnosis; D015870:Gene Expression; D053263:Gene Regulatory Networks; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000097:blood / Q000235:genetics; D019278:Matrix Metalloproteinase 3 / Q000097:blood / Q000235:genetics; D020370:Osteoarthritis, Knee / Q000097:blood / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D011348:Procollagen N-Endopeptidase / Q000097:blood / Q000235:genetics*; D025941:Protein Interaction Mapping; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D003094:Collagen; D051722:ADAM Proteins; D011348:Procollagen N-Endopeptidase; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1; D000071121:ADAMTS4 Protein; C000603918:ADAMTS4 protein, human