title: Micro RNA expression and prognosis in low-grade non-invasive urothelial carcinoma.
abstract: OBJECTIVE
  To analyze a possible correlation between a miRNA expression profile and important prognostic factors for pTa urothelial carcinomas (UC), including tumor size, multiplicity and episodes of recurrence.
  
  
  METHODS
  Thirty low-grade non-invasive pTa bladder UC from patients submitted to transurethral resection were studied, in a mean follow-up of 17.7 months. As controls, we used normal bladder tissue from five patients submitted to retropubic prostatectomy to treat benign prostatic hyperplasia. Extraction, cDNA and amplification were performed for 14 miRNAs (miR-100, -10a, -21, -205, -let7c, -143, -145, -221, -223, -15a, -16, -199a and -452) using specific kits, and RNU-43 and -48 were used as endogenous controls. Statistical tests were used to compare tumor size, multiplicity and episodes of recurrence with miRNAs expression profiles.
  
  
  RESULTS
  There was a marginal correlation between multiplicity and miR-let7c over-expression. For all others miRNA no correlation between their expression and prognostic factors was found.
  
  
  CONCLUSIONS
  We did not find differences for miRNAs expression profiles associated with prognostic factors in tumor group studied. The majority of miRNAs are down-regulated, except mir-10a, over-expressed in most of cases, seeming to have increased levels as tumor with more unfavorable prognostic factors. More studies are needed in order to find a miRNA profile able to provide prognosis in pTa UC to be used in clinical practice.
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Nelson
authors.initials: N
authors.lastname: Dip
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Sabrina T
authors.initials: ST
authors.lastname: Reis
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Abe
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Nayara I
authors.initials: NI
authors.lastname: Viana
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Denis R
authors.initials: DR
authors.lastname: Morais
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Caio M
authors.initials: CM
authors.lastname: Moura
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Betina
authors.initials: B
authors.lastname: Katz
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Iran A
authors.initials: IA
authors.lastname: Silva
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Miguel
authors.initials: M
authors.lastname: Srougi
authors.affiliation: Laboratory of Medical Investigation, Urology Department - LIM55, University of Sao Paulo Medical School, Sao Paulo, Brazil.
authors.forename: Katia R M
authors.initials: KR
authors.lastname: Leite
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012016:Reference Values; D018709:Statistics, Nonparametric; D047368:Tumor Burden / Q000235:genetics; D014516:Ureteral Neoplasms / Q000235:genetics* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs