title: Zipper-interacting protein kinase interacts with human cell division cycle 14A phosphatase.
abstract: Zipper‑interacting protein kinase (ZIPK) is a novel serine/threonine protein kinase and a member of a large family of protein kinases, known as the death‑associated protein kinases. However, the function of ZIPK has yet to be fully elucidated, as few physiological substrates have currently been identified. In the present study, a yeast two‑hybrid screen was used and the human cell division cycle 14A (HsCdc14A) phosphatase was identified as a novel ZIPK binding protein. To the best of our knowledge, this is the first study to report the interaction between these proteins. The interaction between ZIPK and HsCdc14A was confirmed by in vitro experiments. In addition, ZIPK‑mediated phosphorylation was shown to activate the phosphatase activity of HsCdc14A. These findings indicated that ZIPK may also be involved in the regulation of the cell cycle in human cells, by interacting with HsCdc14A.
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Endocrinology and Metabolism, International Healthcare Center, Second Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Zi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Endocrinology, City University of Macau, Macau 000853, P.R. China.
authors.forename: Mancheong
authors.initials: M
authors.lastname: Leong
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Endocrinology, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200000, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Renming
authors.initials: R
authors.lastname: Hu
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology at Fudan University, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D064550:Death-Associated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D017027:Protein Tyrosine Phosphatases; D014162:Transfection; D020798:Two-Hybrid System Techniques
chemical_list: C579101:DAPK3 protein, human; D064550:Death-Associated Protein Kinases; D010744:Phosphoric Monoester Hydrolases; C120902:CDC14A protein, human; D017027:Protein Tyrosine Phosphatases