title: Rapid Determination of Bile Acids in Bile from Various Mammals by Reversed-Phase Ultra-Fast Liquid Chromatography.
abstract: A valid and efficient reversed-phase ultra-fast liquid chromatography method was developed for the simultaneous determination of 13 bile acids in the bile of three mammal species, including rat, pig and human gallstone patients. Chromatographic separation was performed with a Shim-pack XR-ODS column, and the mobile phase consisted of acetonitrile and potassium phosphate buffer (pH 2.6) at a flow rate of 0.5 mL min(-1). The linear detection range of most bile acids ranged from 2 to 600 ng µL(-1) with a good correlation coefficient (>0.9995). The precision of each bile acid was <1.8% for intraday and <4.8% for interday. All bile acids were separated in 15 min with satisfactory resolution, and the total analysis time was 18 min, including equilibration. The method was successfully applied in rapid screening of bile samples from the three mammals. Significant metabolic frameworks of bile acids among various species were observed, whereas considerable quantitative variations in both inter- and intraspecies were also observed, especially for gallstone patients. Our results suggest that detecting the change of bile acid profiles could be applied for the diagnosis of gallstone disease.
authors.affiliation: Pharmacy Administration and Clinical Pharmacy of School of Pharmaceutical Science, Peking University, Xueyuan Road Haidian District, Beijing 100191, China Inner Mongolia Autonomous Region People's Hospital, Inner Mongolia Autonomous Region, Hohhot 010010, China.
authors.forename: Gu Leng Ri
authors.initials: GL
authors.lastname: Si
authors.affiliation: Pharmacy Administration and Clinical Pharmacy of School of Pharmaceutical Science, Peking University, Xueyuan Road Haidian District, Beijing 100191, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Yao
authors.affiliation: Pharmacy Administration and Clinical Pharmacy of School of Pharmaceutical Science, Peking University, Xueyuan Road Haidian District, Beijing 100191, China srgl985@126.com.
authors.forename: Luwen
authors.initials: L
authors.lastname: Shi
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000191:economics / Q000379:methods*; D042882:Gallstones / Q000175:diagnosis*; D006801:Humans; D051381:Rats; D012680:Sensitivity and Specificity; D013552:Swine
chemical_list: D001647:Bile Acids and Salts