title: KLF1 stabilizes GATA-1 and TAL1 occupancy in the human β-globin locus.
abstract: KLF1 is an erythroid specific transcription factor that binds to regulatory regions of erythroid genes. Binding sites of KLF1 are often found near binding sites of GATA-1 and TAL1. In the β-globin locus, KLF1 is required for forming active chromatin structure, although its role is unclear. To explore the role of KLF1 in transcribing the human γ-globin genes, we stably reduced the expression of KLF1 in erythroid K562 cells, compromising its association in the β-globin locus. The γ-globin transcription was reduced with disappearance of active chromatin structure of the locus in the KLF1 knockdown cells. Interestingly, GATA-1 and TAL1 binding was reduced in the β-globin locus, even though their expressions were not affected by KLF1 knockdown. The KLF1-dependent GATA-1 and TAL1 binding was observed in the adult locus transcribing the β-globin gene and in several erythroid genes, where GATA-1 occupancy is independent from TAL1. These results indicate that KLF1 plays a role in facilitating and/or stabilizing GATA-1 and TAL1 occupancy in the erythroid genes, contributing to the generation of active chromatin structure such as histone acetylation and chromatin looping.
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Yea Woon
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jangmi
authors.initials: J
authors.lastname: Yun
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Jongo
authors.initials: J
authors.lastname: Shin
authors.affiliation: Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Republic of Korea. Electronic address: kimaeri@pusan.ac.kr.
authors.forename: AeRi
authors.initials: A
authors.lastname: Kim
mesh_terms: D000107:Acetylation; D051792:Basic Helix-Loop-Helix Proteins / Q000096:biosynthesis / Q000378:metabolism*; D001665:Binding Sites; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D050982:GATA1 Transcription Factor / Q000096:biosynthesis / Q000378:metabolism*; D006657:Histones / Q000235:genetics; D006801:Humans; D020014:K562 Cells; D051741:Kruppel-Like Transcription Factors / Q000096:biosynthesis / Q000378:metabolism*; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D055544:beta-Globins / Q000235:genetics / Q000378:metabolism; D055545:gamma-Globins / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002843:Chromatin; D050982:GATA1 Transcription Factor; D006657:Histones; D051741:Kruppel-Like Transcription Factors; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D055544:beta-Globins; D055545:gamma-Globins; C494688:GATA1 protein, human; C080774:erythroid Kruppel-like factor; C067143:TAL1 protein, human
keywords: Chromatin structure; GATA-1; Human β-globin locus; KLF1; TAL1