title: Aortic relaxant activity of Crataegus gracilior Phipps and identification of some of its chemical constituents.
abstract: This study focused on the assessment of the vasorelaxant activity of the organic and aqueous extracts obtained from leaves and fruits of a Mexican hawthorn (Crataegus gracilior) on isolated rat aorta, and on the purification and identification of some of their secondary metabolites by the use of chromatographic and spectroscopic techniques. The results obtained showed that the methanol extract has a significantly more potent and effective vasorelaxant effect than the other tested extracts, with an EC50 = 8.69 ± 4.34 µg/mL and an Emax = 94.6% ± 11.30%, values that are close to that of acetylcholine, the positive control. From the same extract, two major triterpenes were isolated and identified as ursolic and corosolic acids by comparison of their experimental NMR spectroscopic data with those reported in the literature. Chlorogenic acid, rutin, quercetin, kaempferol and (+)-catechin were also identified using HPLC coupled with PDAD. All these compounds have already been proven to possess on their own antihypertensive effect and other benefits on cardiovascular diseases and they can support, at least in part, the traditional use of this plant species.
authors.affiliation: Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico.
authors.forename: Abigail
authors.initials: A
authors.lastname: Hernández-Pérez
authors.affiliation: Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico. moubah@uaq.mx.
authors.forename: Moustapha
authors.initials: M
authors.lastname: Bah
authors.affiliation: Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico.
authors.forename: César
authors.initials: C
authors.lastname: Ibarra-Alvarado
authors.affiliation: Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Circuito exterior, Coyoacán 04510, Mexico D.F., Mexico.
authors.forename: José Fausto
authors.initials: JF
authors.lastname: Rivero-Cruz
authors.affiliation: Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Rojas-Molina
authors.affiliation: Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas, Querétaro 76010, Qro., Mexico.
authors.forename: Juana Isela
authors.initials: JI
authors.lastname: Rojas-Molina
authors.affiliation: Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Campus Juriquilla, Avenida de Las Ciencias S/N, Delegación Santa Rosa Jáuregui, Querétaro 76230, Qro., Mexico.
authors.forename: José Alejandro
authors.initials: JA
authors.lastname: Cabrera-Luna
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000502:physiology*; D027825:Crataegus / Q000737:chemistry; D005638:Fruit / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008297:Male; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D017208:Rats, Wistar; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology; D014664:Vasodilation; D014665:Vasodilator Agents / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; D014665:Vasodilator Agents; D011794:Quercetin