title: Strengthening the growth of Rubrivivax gelatinosus in sewage purification through ferric ion regulated photophosphorylation and respiration.
abstract: Rubrivivax gelatinosus has the potential of biomass resource recycling combined with sewage purification. However, low biomass production and yield restricts the potential for sewage purification. Thus, this research investigated the improvement of biomass production and yield and organics reduction by Fe(3+) in R. gelatinosus wastewater treatment. Results showed that 10-30 mg/L Fe(3+) improved biomass yield in wastewater to a level found in culture medium. With optimal dosage (20 mg/L), biomass production reached 4,300 mg/L, which was 1.67 times that of the control group. Biomass yield was improved by 43.3%. Chemical oxygen demand (COD) removal reached above 91%. Hydraulic retention time was shortened by 25%. Mechanism analysis indicated that Fe(3+) enhanced the succinate and NADH dehydrogenase activities and, bacteriochlorophyll content in three energy metabolism pathways. These effects then enhanced adenosine triphosphate (ATP) production, which led to more biomass accumulation and COD removal. With 20 mg/L Fe(2+) dosage, succinate and NADH dehydrogenase, coproporphyrinogen III oxidase activities, bacteriochlorophyll content and ATP production were improved, respectively, by 48.4, 50.8, 50, 67 and 56% compared to those of the control group.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China E-mail: woacademic@163.com; School of Environmental Science and Engineering, Sun Yat-Sen University, Guangzhou 510275, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China E-mail: woacademic@163.com.
authors.forename: Jian-zheng
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China E-mail: woacademic@163.com.
authors.forename: Cong
authors.initials: C
authors.lastname: Du
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China E-mail: woacademic@163.com.
authors.forename: Qing-yue
authors.initials: QY
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China E-mail: woacademic@163.com.
authors.forename: Xian-shu
authors.initials: XS
authors.lastname: Liu
authors.affiliation: School of Qiaoxing Light Industry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001429:Bacteriochlorophylls / Q000378:metabolism; D020562:Betaproteobacteria / Q000254:growth & development* / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D019069:Cell Respiration; D007501:Iron / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010785:Photophosphorylation; D059027:Recycling; D012722:Sewage; D013385:Succinate Dehydrogenase / Q000378:metabolism; D018505:Waste Management / Q000379:methods*; D062065:Wastewater
chemical_list: D000255:Adenosine Triphosphate; D001429:Bacteriochlorophylls; D007501:Iron; D009245:NADH Dehydrogenase; D012722:Sewage; D013385:Succinate Dehydrogenase; D062065:Wastewater