title: [Clinical significance of CIP2A expression in bladder cancer].
abstract: OBJECTIVE
  To explore the expression of cancerous inhibitor of PP2A (CIP2A) and evaluate its role in bladder cancer.
  
  
  METHODS
  RT-PCR was used to detect the expression of CIP2A mRNA from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue. The CIP2A protein expression levels in 99 cases of patients with bladder cancer and 12 cases of normal tissue was detected by immunohistochemical staining . And the serum contents of CIP2A protein of 38 patients with bladder cancer and 40 normal controls were detected by ELISA.
  
  
  RESULTS
  The expression of CIP2A mRNA was detected in 29/38 cases (76.32%) of bladder cancer. And there was no expression in normal tissue (P < 0.05). The positive rate of CIP2A protein was 63.64% in 99 cases of bladder cancer tissues and no expression detected in normal tissues(P < 0.05). ELISA results showed that the serum content of CIP2A in patients with bladder cancer was significantly higher than that in normal controls (median:0.015 2 vs 0.001 8 ng/L, P < 0.05).
  
  
  CONCLUSIONS
  The tissue and serum expressions of CIP2A in patients with bladder cancer are higher than those in normal controls. And CIP2A may be used as an indicator of the biological behavior of bladder cancer.
authors.affiliation: Department of Urology, Second Hospital, Shandong University, Shandong University Urologic Institute, Jinan 250033, China.
authors.forename: Tianjia
authors.initials: T
authors.lastname: Ma
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Pan
authors.initials: P
authors.lastname: Xiao
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yutang
authors.initials: Y
authors.lastname: Li
authors.forename: Peikun
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Pathogen Biology, Shandong University, Jinan 250012, China. Email: zyb@sdu.edu.cn.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D001324:Autoantigens; D014408:Biomarkers, Tumor; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D001749:Urinary Bladder Neoplasms
chemical_list: D001324:Autoantigens; D014408:Biomarkers, Tumor; C500266:CIP2A protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger