title: Heparin nanoparticles for β amyloid binding and mitigation of β amyloid associated cytotoxicity.
abstract: Accumulation of β amyloid (Aβ) in the brain is believed to play a key role in the pathology of Alzheimer's disease. Glycosaminoglycans on surface of neuronal cells can serve as nucleation sites to promote plaque formation on cell surface. To mimic this process, magnetic nanoparticles coated with heparin have been synthesized. The heparin nanoparticles were demonstrated to bind with Aβ through a variety of techniques including enzyme-linked immunosorbent assay, gel electrophoresis, and thioflavin T assay. The nanoparticle exhibited little toxicity to neuronal cells and at the same time can effectively protect them from Aβ induced cytotoxicity. These results suggest that heparin nanoparticles can be a very useful tool for Aβ studies.
authors.affiliation: Department of Chemistry, 578 S. Shaw Lane, Room 426, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Chemistry, 578 S. Shaw Lane, Room 426, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Hovig
authors.initials: H
authors.lastname: Kouyoumdjian
authors.affiliation: Department of Radiology, Michigan State University, East Lansing, MI 48824, USA; Department of Psychology, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: David C
authors.initials: DC
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, 578 S. Shaw Lane, Room 426, Michigan State University, East Lansing, MI 48824, USA. Electronic address: xuefei@chemistry.msu.edu.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Huang
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity*; D000818:Animals; D005290:Ferric Compounds / Q000737:chemistry; D006493:Heparin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry*; D009474:Neurons / Q000187:drug effects; D011485:Protein Binding; D055503:Protein Multimerization / Q000187:drug effects; D017433:Protein Structure, Secondary
chemical_list: D016229:Amyloid beta-Peptides; D005290:Ferric Compounds; C000499:ferric oxide; D006493:Heparin
keywords: Amyloid β; Binding study; Cytotoxicity; Heparin; Nanoparticle