title: TIP30 inhibits oligodendrocyte precursor cell differentiation via cytoplasmic sequestration of Olig1.
abstract: Differentiation of oligodendrocyte precursor cells (OPCs) is a prerequisite for both developmental myelination and adult remyelination in the central nervous system. The molecular mechanisms underlying OPC differentiation remain largely unknown. Here, we show that the thirty-kDa HIV-1 Tat interacting protein (TIP30) is a negative regulator in oligodendrocyte development. The TIP30(-/-) mice displayed an increased myelin protein level at postnatal day 14 and 21. By using a primary OPC culture system, we demonstrated that overexpression of TIP30 dramatically inhibited the stage progression of differentiating OPCs, while knockdown of TIP30 enhanced the differentiation of oligodendroglial cells remarkably. Moreover, overexpression of TIP30 was found to sequester the transcription factor Olig1 in the cytoplasm and weaken its nuclear translocation due to the interaction between TIP30 and Olig1, whereas knockdown of TIP30 led to more Olig1 localized in the nucleus in the initiation stage during OPC differentiation. In the cuprizone-induced demyelination model, there was a dramatic increase in NG2-expressing cells with nuclear location of Olig1 in the corpus callosum during remyelination. In contrast, within chronic demyelinated lesions in multiple sclerosis, TIP30 was abnormally expressed in NG2-expressing cells, and few nuclear Olig1 was observed in these cells. Taken together, our findings suggest that TIP30 plays a negative regulatory role in oligodendroglial differentiation.
authors.affiliation: Institute of Neuroscience and MOE Key Laboratory of Molecular Neurobiology, Neuroscience Research Center of Changzheng Hospital, Second Military Medical University, Shanghai, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yang
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Cui
authors.initials: C
authors.lastname: Li
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Liu
authors.forename: Mingdong
authors.initials: M
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Shao
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Aijun
authors.initials: A
authors.lastname: Huang
authors.forename: Cheng
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000941:Antigens / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics* / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D003337:Corpus Callosum / Q000166:cytology; D003593:Cytoplasm / Q000378:metabolism*; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009186:Myelin Sheath / Q000378:metabolism; D009413:Nerve Fibers, Myelinated / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D011509:Proteoglycans / Q000378:metabolism; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*; D025521:Tumor Suppressor Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D011509:Proteoglycans; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; D000099216:Chondroitin Sulfate Proteoglycan 4; C494786:Olig1 protein, mouse; C525546:Tip30 protein, mouse
keywords: Olig1; TIP30; differentiation; multiple sclerosis; oligodendrocyte