title: Genome-wide association and biological pathway analysis for milk-fat composition in Danish Holstein and Danish Jersey cattle.
abstract: BACKGROUND
  The milk fat profile of the Danish Holstein (DH) and Danish Jersey (DJ) show clear differences. Identification of the genomic regions, genes and biological pathways underlying the milk fat biosynthesis will improve the understanding of the biology underlying bovine milk fat production and may provide new possibilities to change the milk fat composition by selective breeding. In this study a genome wide association scan (GWAS) in the DH and DJ was performed for a detailed milk fatty acid (FA) profile using the HD bovine SNP array and subsequently a biological pathway analysis based on the SNP data was performed.
  
  
  RESULTS
  The GWAS identified in total 1,233 SNPs (FDR < 0.10) spread over 18 chromosomes for nine different FA traits for the DH breed and 1,122 SNPs (FDR < 0.10) spread over 26 chromosomes for 13 different FA traits were detected for the DJ breed. Of these significant SNPs, 108 SNP markers were significant in both DH and DJ (C14-index, BTA26; C16, BTA14; fat percentage (FP), BTA14). This was supported by an enrichment test. The QTL on BTA14 and BTA26 represented the known candidate genes DGAT and SCD. In addition we suggest ACSS3 to be a good candidate gene for the QTL on BTA5 for C10:0 and C15:0. In addition, genetic correlations between the FA traits within breed showed large similarity across breeds. Furthermore, the biological pathway analysis revealed that fat digestion and absorption (KEGG04975) plays a role for the traits FP, C14:1, C16 index and C16:1.
  
  
  CONCLUSIONS
  There was a clear similarity between the underlying genetics of FA in the milk between DH and DJ. This was supported by the fact that there was substantial overlap between SNPs for FP, C14 index, C14:1, C16 index and C16:1. In addition genetic correlations between FA showed a similar pattern across DH and DJ. Furthermore the biological pathway analysis suggested that fat digestion and absorption KEGG04975 is important for the traits FP, C14:1, C16 index and C16:1.
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Center for Quantitative Genetics and Genomics, Blichers Allé 20, P,O, Box 50, DK-8830 Tjele, Denmark. bart.buitenhuis@mbg.au.dk.
authors.forename: Bart
authors.initials: B
authors.lastname: Buitenhuis
authors.forename: Luc L G
authors.initials: LL
authors.lastname: Janss
authors.forename: Nina A
authors.initials: NA
authors.lastname: Poulsen
authors.forename: Lotte B
authors.initials: LB
authors.lastname: Larsen
authors.forename: Mette K
authors.initials: MK
authors.lastname: Larsen
authors.forename: Peter
authors.initials: P
authors.lastname: Sørensen
mesh_terms: D000818:Animals; D002417:Cattle; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D016678:Genome; D055106:Genome-Wide Association Study; D005838:Genotype; D007774:Lactation / Q000235:genetics; D008892:Milk / Q000378:metabolism*; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D013230:Stearoyl-CoA Desaturase / Q000235:genetics
chemical_list: D005227:Fatty Acids; D013230:Stearoyl-CoA Desaturase; D051048:Diacylglycerol O-Acyltransferase