title: PDGF-BB protects mitochondria from rotenone in T98G cells.
abstract: Rotenone is one of the most-studied neurotoxic substances as it induces oxidative stress processes both in cellular and animal models. Rotenone affects ATP generation, reactive oxygen species (ROS) production, and mitochondrial membrane potential in neurons and astrocyte-like cells. Previous epidemiologic studies have supported the role of neurotrophic factors such as BDNF and GDNF in neuroprotection mainly in neurons; however, only very few studies have focused on the importance of astrocytic protection in neurodegenerative models. In the present study, we assessed the neuroprotective effects of PDGF-BB against toxicity induced by rotenone in the astrocytic-like model of T98G human glioblastoma cell line. Our results demonstrated that pretreatment with PDGF-BB for 24 h increased cell viability, preserved nuclear morphology and mitochondrial membrane potential following stimulation with rotenone, and reduced ROS production nearly to control conditions. These observations were accompanied by important morphological changes induced by rotenone and that PDGF-BB was able to preserve cellular morphology under this toxic stimuli. These findings indicated that PDGF-BB protects mitochondrial functions, and may serve as a potential therapeutic strategy in rotenone-induced oxidative damage in astrocytes.
authors.affiliation: Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Cabezas
authors.forename: Marcos Fidel
authors.initials: MF
authors.lastname: Avila
authors.forename: Janneth
authors.initials: J
authors.lastname: González
authors.forename: Ramon Santos
authors.initials: RS
authors.lastname: El-Bachá
authors.forename: George E
authors.initials: GE
authors.lastname: Barreto
mesh_terms: D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism; D000077214:Becaplermin; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012402:Rotenone / Q000633:toxicity*
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D017382:Reactive Oxygen Species; D012402:Rotenone; D000077214:Becaplermin