title: Molecularly imprinted hollow sphere array for the sensing of proteins.
abstract: Mono-dispersed molecularly imprinted hollow spheres (MIHSs) for hemoglobin (Hb) were prepared by employing silica nanospheres as the sacrificial templates. The obtained hollow spheres with uniform particle size of 360 nm in diameter were characterized by transmission electron microscopy. The outstanding affinities of these MIHSs to the target protein were confirmed by adsorption experiment in aqueous solution. Adsorption equilibrium was achieved within 10 min while the binding capacity (Qmax ) of Hb was 8.84 µmol g(-1) at pH7.0. Furthermore, the MIHSs were successfully assembled into a closely-packed 3D colloidal array. The molecularly imprinted hollow sphere array (MIHSA) can selectively recognize Hb. As the concentration of Hb increased, the structure color of the MIHSA changed from blue to green, and turn to white finally with maximum red shift for 43 nm. The MIHSA showed promising potential for the naked-eye detection of target Hb. Reflection spectra of the MIHSA in response to different Hb concentrations (0.075-15 µmol L(-1) ).
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China. minxue@bit.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Xue
authors.affiliation: Department of Chemistry, University of California, Irvine, California, 92697, United States.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Shea
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China. m_zihui@yahoo.com.
authors.forename: Zihui
authors.initials: Z
authors.lastname: Meng
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Zequn
authors.initials: Z
authors.lastname: Yan
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering & Environment, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xue
authors.affiliation: School of Life Science, Beijing Institute of Technology, Beijing, 100081, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D003116:Color; D006454:Hemoglobins / Q000032:analysis* / Q000737:chemistry; D054802:Molecular Imprinting; D054118:Nanospheres / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D012822:Silicon Dioxide
keywords: biosensor; hemoglobin; molecularly imprinted polymer; naked-eye detection; photonic crystal