title: Hydrogen supplementation of preservation solution improves viability of osteochondral grafts.
abstract: Allogenic osteochondral tissue (OCT) is used for the treatment of large cartilage defects. Typically, OCTs collected during the disease-screening period are preserved at 4°C; however, the gradual reduction in cell viability during cold preservation adversely affects transplantation outcomes. Therefore, improved storage methods that maintain the cell viability of OCTs are needed to increase the availability of high-quality OCTs and improve treatment outcomes. Here, we evaluated whether long-term hydrogen delivery to preservation solution improved the viability of rat OCTs during cold preservation. Hydrogen-supplemented Dulbecco's Modified Eagles Medium (DMEM) and University of Wisconsin (UW) solution both significantly improved the cell viability of OCTs during preservation at 4°C for 21 days compared to nonsupplemented media. However, the long-term cold preservation of OCTs in DMEM containing hydrogen was associated with the most optimal maintenance of chondrocytes with respect to viability and morphology. Our findings demonstrate that OCTs preserved in DMEM supplemented with hydrogen are a promising material for the repair of large cartilage defects in the clinical setting.
authors.affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yamada
authors.affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Kentaro
authors.identifier: 0000-0001-5148-914X
authors.initials: K
authors.lastname: Uchida
authors.affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Onuma
authors.affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kuzuno
authors.affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Masanobu
authors.initials: M
authors.lastname: Ujihira
authors.affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Gen
authors.identifier: 0000-0001-6500-9004
authors.initials: G
authors.lastname: Inoue
authors.affiliation: MiZ Co., Ltd., 1-16-5 Zenko, Fujisawa, Kanagawa 251-0871, Japan.
authors.forename: Bunpei
authors.identifier: 0000-0003-1972-9646
authors.initials: B
authors.lastname: Sato
authors.affiliation: MiZ Co., Ltd., 1-16-5 Zenko, Fujisawa, Kanagawa 251-0871, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Kurokawa
authors.affiliation: Department of Medical Engineering and Technology, School of Allied Health Science, Kitasato University, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Rina
authors.initials: R
authors.lastname: Sakai
authors.affiliation: Department of Orthopedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku, Kitasato, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Takaso
mesh_terms: D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015925:Cryopreservation; D006859:Hydrogen / Q000494:pharmacology*; D008297:Male; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013194:Staining and Labeling; D014022:Tissue Survival / Q000187:drug effects*
chemical_list: D019188:Organ Preservation Solutions; D006859:Hydrogen