title: Analysis of resistance to Cry1Ac in field-collected pink bollworm, Pectinophora gossypiella (Lepidoptera:Gelechiidae), populations.
abstract: High survivorship of pink bollworrm, Pectinophora gossypiella in bolls of Bollgard® cotton hybrids and resistance to Cry1Ac protein, expressed in Bollgard cotton were reported in field-populations collected from the state of Gujarat (western India) in 2010. We have found Cry1Ac-resistance in pink bollworm populations sourced from Bollgard and non-Bt cotton fields in the adjoining states of Maharashtra and Madhya Pradesh in Central India. Further, we observed reduced binding of labeled Cry1Ac protein to receptors localized on the brush-border membrane of pink bollworm larval strains with high tolerance to Cry1Ac. These strains were sourced from Bollgard and conventional cotton fields. A pooled Cry1Ac-resistant strain, further selected on Cry1Ac diet also showed significantly reduced binding to Cry1Ac protein. The reduced binding of Cry1Ac to receptors could be an underlying mechanism for the observed resistance in pink bollworm populations feeding on Bollgard hybrids.
authors.affiliation: a International Centre For Genetic Engineering and Biotechnology ; New Delhi , India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Ojha
authors.forename: K Sowjanya
authors.initials: KS
authors.lastname: Sree
authors.forename: Bindiya
authors.initials: B
authors.lastname: Sachdev
authors.forename: M A
authors.initials: MA
authors.lastname: Rashmi
authors.forename: K C
authors.initials: KC
authors.lastname: Ravi
authors.forename: P J
authors.initials: PJ
authors.lastname: Suresh
authors.forename: Komarlingam S
authors.initials: KS
authors.lastname: Mohan
authors.forename: Raj K
authors.initials: RK
authors.lastname: Bhatnagar
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism; D004731:Endotoxins / Q000378:metabolism; D003368:Gossypium / Q000235:genetics / Q000469:parasitology*; D006460:Hemolysin Proteins / Q000378:metabolism; D007194:India; D019476:Insect Proteins / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000378:metabolism; D007814:Larva / Q000502:physiology; D009036:Moths / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D019476:Insect Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: BBMV, brush border membrane vesicle; Bollgard®; Bt, Bacillus thuringiensis; Cry1Ac-resistance; IRM, insect resistance management; MRC, Monsanto Research Center; NKJ, Nandurbar-Khargaon-Jalgaon; PBS, phosphate buffered saline; PBW, pink bollworm; brush border membrane vesicle, Bt cotton; pink bollworm; receptor binding