title: A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction.
abstract: A strategy for T4 polynucleotide kinase activity detection was proposed based on a β-cyclodextrin polymer (polyβ-CD) and an exonuclease reaction. The fluorescence of pyrene enhanced by more than 10 times in the presence of polyβ-CD, and a simple detection of T4 PNK was achieved with a detection limit of 0.02 units per mL.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. kmwang@hnu.edu.cn yangxiaohai@hnu.edu.cn.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Song
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Leiliang
authors.initials: L
authors.lastname: He
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Qing
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D017122:Bacteriophage T4 / Q000201:enzymology*; D057075:Enzyme Assays / Q000379:methods*; D005092:Exonucleases / Q000378:metabolism*; D005453:Fluorescence; D057230:Limit of Detection; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism*; D011721:Pyrenes / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D011721:Pyrenes; D047392:beta-Cyclodextrins; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D005092:Exonucleases