title: Proteomics of effector-triggered immunity (ETI) in plants.
abstract: Effector-triggered immunity (ETI) was originally termed gene-for-gene resistance and dates back to fundamental observations of flax resistance to rust fungi by Harold Henry Flor in the 1940s. Since then, genetic and biochemical approaches have defined our current understanding of how plant "resistance" proteins recognize microbial effectors. More recently, proteomic approaches have expanded our view of the protein landscape during ETI and contributed significant advances to our mechanistic understanding of ETI signaling. Here we provide an overview of proteomic techniques that have been used to study plant ETI including both global and targeted approaches. We discuss the challenges associated with ETI proteomics and highlight specific examples from the literature, which demonstrate how proteomics is advancing the ETI research field.
authors.affiliation: a Department of Cell & Systems Biology; University of Toronto; Toronto, ON Canada.
authors.forename: Brenden
authors.initials: B
authors.lastname: Hurley
authors.forename: Rajagopal
authors.initials: R
authors.lastname: Subramaniam
authors.forename: David S
authors.initials: DS
authors.lastname: Guttman
authors.forename: Darrell
authors.initials: D
authors.lastname: Desveaux
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D009154:Mutation; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity; D010940:Plant Proteins / Q000378:metabolism*; D010944:Plants / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D040901:Proteomics / Q000379:methods; D044224:Pseudomonas syringae / Q000276:immunology / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D010940:Plant Proteins
keywords: Effector-Triggered Immunity; NB-LRR proteins; Proteomics; Pseudomonas syringae; Type III effector