title: [Effects of fibroblasts on the proliferation and migratory capacity of renal cell carcinoma cell lines and sensitivity to everolimus].
abstract: OBJECTIVE
  To observe the effects of normal human renal fibroblast (NHRF) on renal cell carcinoma cell lines.
  
  
  METHODS
  Renal cell carcinoma (RCC) cell lines 786-O and Caki-1 were co-cultured with NHRF for assessing the proliferation and migratory capacities of renal cell carcinoma cell lines and the sensitivity to everolimus.
  
  
  RESULTS
  Co-culturing with NHRF substantially increased the proliferation capacity of both RCC cell lines (786-O: 2.35 ± 0.05 vs1.93 ± 0.15, P = 0.01;Caki-1: 2.35 ± 0.21 vs 1.24 ± 0.11, P = 0.001). Similarly the migratory capacities of both cell lines became significantly enhanced after co-culturing (786-O: 1.53 ± 0.11 vs 0.98 ± 0.11, P = 0.04; Caki-1: 1.53 ± 0.11vs 0.98 ± 0.11, P = 0.04) compared with untreated control. Furthermore, the sensitivities of both cell lines to everolimus (1, 5, 10 µmol/L) dramatically decreased in those pre-co-cultured with NHRF (786-O:P value 0.04, 0.35, 0.18); Caki-1: P value 0.02, 0.03, 0.024).
  
  
  CONCLUSIONS
  NHRF promotes the proliferation and migration capacities of 786-O and Caki-1. And it may be involved in the resistance of RCC to everolimus.
authors.affiliation: Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai 200127, China. Email: hyrrenji2@aliyun.com.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Huang
mesh_terms: D002292:Carcinoma, Renal Cell; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D000068338:Everolimus; D005347:Fibroblasts; D006801:Humans; D007668:Kidney; D007680:Kidney Neoplasms; D020123:Sirolimus / Q000031:analogs & derivatives
chemical_list: D000068338:Everolimus; D020123:Sirolimus