title: Isolation and structural elucidation of antiproliferative compounds of lipidic fractions from white shrimp muscle (Litopenaeus vannamei).
abstract: Shrimp is one of the most popular seafood items worldwide, and has been reported as a source of chemopreventive compounds. In this study, shrimp lipids were separated by solvent partition and further fractionated by semi-preparative RP-HPLC and finally by open column chromatography in order to obtain isolated antiproliferative compounds. Antiproliferative activity was assessed by inhibition of M12.C3.F6 murine cell growth using the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay. The methanolic fraction showed the highest antiproliferative activity; this fraction was separated into 15 different sub-fractions (M1-M15). Fractions M8, M9, M10, M12, and M13 were antiproliferative at 100 µg/mL and they were further tested at lower concentrations. Fractions M12 and M13 exerted the highest growth inhibition with an IC50 of 19.5 ± 8.6 and 34.9 ± 7.3 µg/mL, respectively. Fraction M12 was further fractionated in three sub-fractions M12a, M12b, and M12c. Fraction M12a was identified as di-ethyl-hexyl-phthalate, fraction M12b as a triglyceride substituted by at least two fatty acids (predominantly oleic acid accompanied with eicosapentaenoic acid) and fraction M12c as another triglyceride substituted with eicosapentaenoic acid and saturated fatty acids. Bioactive triglyceride contained in M12c exerted the highest antiproliferative activity with an IC50 of 11.33 ± 5.6 µg/mL. Biological activity in shrimp had been previously attributed to astaxanthin; this study demonstrated that polyunsaturated fatty acids are the main compounds responsible for antiproliferative activity.
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Apartado Postal 1658, Hermosillo, Sonora 83000, Mexico. k_rmelita@hotmail.com.
authors.forename: Carmen-María
authors.initials: CM
authors.lastname: López-Saiz
authors.affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de Sonora, Apartado Postal 1685, Hermosillo, Sonora 83000, Mexico. velaz@guayacan.uson.mx.
authors.forename: Carlos
authors.initials: C
authors.lastname: Velázquez
authors.affiliation: Unidad de Servicios de Apoyo en Resolución Analítica, Universidad Veracruzana, Xico, Veracruz 91190, Mexico. javmartinez@uv.mx.
authors.forename: Javier
authors.initials: J
authors.lastname: Hernández
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Apartado Postal 1658, Hermosillo, Sonora 83000, Mexico. fcinco@guayacan.uson.mx.
authors.forename: Francisco-Javier
authors.initials: FJ
authors.lastname: Cinco-Moroyoqui
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Apartado Postal 1658, Hermosillo, Sonora 83000, Mexico. mplascencia@guayacan.uson.mx.
authors.forename: Maribel
authors.initials: M
authors.lastname: Plascencia-Jatomea
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Apartado Postal 1658, Hermosillo, Sonora 83000, Mexico. rsanchez@guayacan.uson.mx.
authors.forename: Maribel
authors.initials: M
authors.lastname: Robles-Sánchez
authors.affiliation: Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, Apartado Postal 1685, Hermosillo, Sonora 83000, Mexico. lmachi@polimeros.uson.mx.
authors.forename: Lorena
authors.initials: L
authors.lastname: Machi-Lara
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Apartado Postal 1658, Hermosillo, Sonora 83000, Mexico. aburgos@guayacan.uson.mx.
authors.forename: Armando
authors.initials: A
authors.lastname: Burgos-Hernández
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D005591:Chemical Fractionation; D003445:Crustacea / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D009132:Muscles / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D008055:Lipids