title: Proteomic profile in glomeruli of type-2 diabetic KKAy mice using 2-dimensional differential gel electrophoresis.
abstract: BACKGROUND
  Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. To search for glomerular proteins associated with early-stage DN, glomeruli of spontaneous type 2 diabetic KKAy mice were analyzed by 2-dimensional differential gel electrophoresis (2D-DIGE).
  
  
  METHODS
  Glomeruli of 20-week spontaneous type 2 diabetic KKAy mice and age-matched C57BL/6 mice were isolated by kidney perfusion with magnetic beads. Proteomic profiles of glomeruli were investigated by using 2D-DIGE and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Western blot analysis was used to confirm the results of proteomics. Immunohistochemical and semi-quantitative analysis were used to confirm the differential expression of prohibitin and annexin A2 in glomeruli.
  
  
  RESULTS
  We identified 19 differentially expressed proteins - 17 proteins were significantly up-regulated and 2 proteins were significantly down-regulated in glomeruli of diabetic KKAy mice. Among them, prohibitin and annexin A2 were up-regulated and Western blot analysis validated the same result in proteomics. Immunohistochemical analysis also revealed up-regulation of prohibitin and annexin A2 in glomeruli of KKAy mice.
  
  
  CONCLUSIONS
  Our findings suggest that prohibitin and annexin A2 may be associated with early-stage DN. Further functional research might help to reveal the pathogenesis of DN.
authors.affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Gang
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Lining
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism; D015153:Blotting, Western; D003928:Diabetic Nephropathies / Q000378:metabolism* / Q000473:pathology; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D010641:Phenotype; D000091402:Prohibitins; D040901:Proteomics / Q000379:methods*; D012097:Repressor Proteins / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D017306:Annexin A2; D000091402:Prohibitins; D012097:Repressor Proteins