title: Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos.
abstract: It has been considered that concentrations of certain amino acids in the egg are not sufficient to fully support embryonic development of modern broilers. In this study we evaluated embryo growth and development with particular emphasis on one of the major components of connective tissue, collagen. Experiments were performed on Ross 308 chicken embryos from 160 fertilised eggs. Experimental solutions of silver nanoparticles (Ag), hydroxyproline solution (Hyp) and a complex of silver nanoparticles with hydroxyproline (AgHyp) were injected into albumen, and embryos were incubated until day 20. An assessment of the mass of embryo and selected organs was carried out followed by measurements of the expression of the key signalling factors' fibroblast growth factor-2 (FGF-2) and vascular endothelial growth factor-A (VEGF-A). Finally, an evaluation of collagen microstructure using scanning electron microscopy was performed. Our results clearly indicate that Hyp, Ag and AgHyp administered in ovo to chicken embryos did not harm embryos. Comparing to the control group, Hyp, Ag and the AgHyp complex significantly upregulated expression of the FGF-2 at the mRNA and protein levels. Moreover, Hyp, Ag and, in particular, the complex of AgHyp significantly increased blood vessel size, cartilage collagen fibre lattice size and bundle thickness. The general conclusion from this study is that AgHyp treatment may help to build a stronger and longer lasting form of collagen fibres.
authors.affiliation: a Division of Nanobiotechnology , Warsaw University of Life Sciences , Warsaw , Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Beck
authors.forename: Anna
authors.initials: A
authors.lastname: Hotowy
authors.forename: Ewa
authors.initials: E
authors.lastname: Sawosz
authors.forename: Marta
authors.initials: M
authors.lastname: Grodzik
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wierzbicki
authors.forename: Marta
authors.initials: M
authors.lastname: Kutwin
authors.forename: Sławomir
authors.initials: S
authors.lastname: Jaworski
authors.forename: André
authors.initials: A
authors.lastname: Chwalibog
mesh_terms: D000818:Animals; D002642:Chick Embryo / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D003331:Coronary Vessels / Q000196:embryology / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006909:Hydroxyproline / Q000008:administration & dosage / Q000494:pharmacology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D010009:Osteochondrodysplasias / Q000378:metabolism*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D012834:Silver; D006909:Hydroxyproline
keywords: chickens; collagen; connective tissue; embryo development; hydroxyproline; nanoparticles; silver