title: High-throughput characterization of protein-RNA interactions.
abstract: RNA-binding proteins (RBPs) are important regulators of eukaryotic gene expression. Genomes typically encode dozens to hundreds of proteins containing RNA-binding domains, which collectively recognize diverse RNA sequences and structures. Recent advances in high-throughput methods for assaying the targets of RBPs in vitro and in vivo allow large-scale derivation of RNA-binding motifs as well as determination of RNA-protein interactions in living cells. In parallel, many computational methods have been developed to analyze and interpret these data. The interplay between RNA secondary structure and RBP binding has also been a growing theme. Integrating RNA-protein interaction data with observations of post-transcriptional regulation will enhance our understanding of the roles of these important proteins.
authors.forename: Kate B
authors.initials: KB
authors.lastname: Cook
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Hughes
authors.forename: Quaid D
authors.initials: QD
authors.lastname: Morris
mesh_terms: D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA
keywords: RBP target identification; RNA secondary structure; RNA-binding proteins; high-throughput sequencing