title: Full genome sequence analysis of a novel adenovirus of rhesus macaque origin indicates a new simian adenovirus type and species.
abstract: Multiple novel simian adenoviruses have been isolated over the past years and their potential to cross the species barrier and infect the human population is an ever present threat. Here we describe the isolation and full genome sequencing of a novel simian adenovirus (SAdV) isolated from the urine of two independent, never co-housed, late stage simian immunodeficiency virus (SIV)-infected rhesus macaques. The viral genome sequences revealed a novel type with a unique genome length, GC content, E3 region and DNA polymerase amino acid sequence that is sufficiently distinct from all currently known human- or simian adenovirus species to warrant classifying these isolates as a novel species of simian adenovirus. This new species, termed Simian mastadenovirus D (SAdV-D), displays the standard genome organization for the genusMastadenoviruscontaining only one copy of the fiber gene which sets it apart from the old world monkey adenovirus species HAdV-G, SAdV-B and SAdV-C.
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Daniel
authors.initials: D
authors.lastname: Malouli
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Grant L
authors.initials: GL
authors.lastname: Howell
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Alfred W
authors.initials: AW
authors.lastname: Legasse
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Christoph
authors.initials: C
authors.lastname: Kahl
authors.affiliation: Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Axthelm
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Scott G
authors.initials: SG
authors.lastname: Hansen
authors.affiliation: Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, United States of America.
authors.forename: Klaus
authors.initials: K
authors.lastname: Früh
keywords: adenovirus; adenovirus species; non-human primate; rhesus macaque; simian adenovirus; virus genome analysis; virus phylogenomics