title: Technologies and Challenges in Proteomic Analysis of ProteinS-acylation.
abstract: ProteinS-acylation (also called palmitoylation) is a pervasive post-translational modification that plays critical roles in regulating protein trafficking, localization, stability, activity, and complex formation. The past decade has witnessed tremendous advances in the study of proteinS-acylation, largely owing to the development of novelS-acylproteomics technologies. In this review, we summarize currentS-acylproteomics approaches, critically review publishedS-acylproteomics studies, and envision future directions for the burgeoningS-acylproteomics field. EmergingS-acylproteomics technologies promise to shed new light on this distinct post-translational modification and facilitate the discovery of new disease mechanisms, biomarkers, and therapeutic targets.
authors.affiliation: Cancer Biology Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA ; Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Cancer Biology Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA ; Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Mingrui
authors.initials: M
authors.lastname: An
authors.affiliation: Cancer Biology Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA ; Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Freeman
authors.affiliation: Cancer Biology Program, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA, USA ; Departments of Surgery and Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
keywords: Acylprotein thioesterase; Click chemistry; DHHC; Mass spectrometry; Palmitoyl acyltransferase; Palmitoylation; Proteomics; S-acylation; Site-specific; Substrate