title: Silencing miR-106b improves palmitic acid-induced mitochondrial dysfunction and insulin resistance in skeletal myocytes.
abstract: MicroRNA‑106b (miR‑106b) is reported to correlate closely with skeletal muscle insulin resistance. In the current study the effect of miR‑106b on palmitic acid (PA)‑induced mitochondrial dysfunction and insulin resistance was investigated in C2C12 myotubes via the silencing of miR‑106b. MiR‑106b expression was increased under PA treatment, while miR‑106b loss of function improved insulin sensitivity by upregulating its target mitofusin‑2 (Mfn2) in C2C12 myocytes. Furthermore, miR‑106b loss of function partly improved mitochondrial morphological lesions and increased the levels of mitochondial DNA and intracellular adenosine triphosphate that had been impaired by PA exposure in C2C12 myocytes. MiR‑106b loss of function attenuated the levels of intracellular reactive oxygen species (ROS), and upregulated the expression levels of the estrogen‑related receptor (ERR)‑α/peroxisome proliferative activated receptor γ coactivator (PGC)‑1α/Mfn2 axis under PA exposure. In addition, miR‑106b negatively regulated skeletal muscle mitochondrial function and insulin sensitivity under PA‑induced insulin resistance by targeting Mfn2, which may be associated with reduced ROS and upregulation of the ERR‑α/PGC‑1α/Mfn2 axis.
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, The 82nd Hospital of the People's Liberation Army, Huai'an, Jiangsu 223001, P.R. China.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhao
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Yuan-Fu
authors.initials: YF
authors.lastname: Gao
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Fan
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Meng-Yuan
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Cai
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Zheng-Kun
authors.initials: ZK
authors.lastname: Xia
authors.affiliation: Department of Pediatrics, Jinling Hospital, Nanjing University, School of Medicine, Nanjing, Jiangsu 210002, P.R. China.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Gao
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000378:metabolism*; D019308:Palmitic Acid / Q000494:pharmacology*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species / Q000378:metabolism; D011960:Receptors, Estrogen / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D000255:Adenosine Triphosphate; D035683:MicroRNAs; D019308:Palmitic Acid; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species; D011960:Receptors, Estrogen; D014157:Transcription Factors; D000097983:ERRalpha Estrogen-Related Receptor; C543896:Mirn106 microRNA, mouse; C485290:Ppargc1a protein, mouse