title: Ultrasensitive immunochromatographic assay for the simultaneous detection of five chemicals in drinking water.
abstract: In this paper, we describe the development of a multicomponent lateral-flow assay based on an antibody-antigen reaction for the rapid and simultaneous detection of trace contaminants in water, including a heavy metal, algal toxin, antibiotic, hormone, and pesticide. The representative analytes chosen for the study were lead (Pb(II), microcystin-leucine-arginine (MC-LR), chloramphenicol (CAP), testosterone (T), and chlorothalonil (CTN). Five different antigens were immobilized separately in five test lines on a nitrocellulose membrane. The monoclonal antibodies specifically recognized the corresponding antigens, and there was no cross-reactivity between the antibodies in the detection assay. Samples or standards containing the five analytes were preincubated with the freeze-dried colloidal-gold-labeled monoclonal antibody conjugates to improve the sensitivity of the assay. The results were obtained within 20min with a paper-based sensor. The cut-off values for the strip test were 4ng/mL for Pb(II), 1ng/mL for MC-LR, 0.1ng/mL for CAP, 5ng/mL for T, and 5ng/mL for CTN. The assay was evaluated using spiked water samples, and the accuracy and reproducibility of the results were good. In summary, this lateral-flow device provides an effective and rapid method for the onsite detection of multiple contaminants in water samples, with no treatment or devices required.
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Changrui
authors.initials: C
authors.lastname: Xing
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Song
authors.affiliation: Huaian Entry-Exit Inspection and Quarantine Bureau, Huaian 223001, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China. Electronic address: kuangh@jiangnan.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.affiliation: State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, JiangSu 214122, PR China.
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
mesh_terms: D000728:Androgens / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000032:analysis; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D001427:Bacterial Toxins / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D002701:Chloramphenicol / Q000032:analysis; D002846:Chromatography, Affinity / Q000379:methods* / Q000706:statistics & numerical data; D003429:Cross Reactions; D060766:Drinking Water / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000032:analysis; D006046:Gold; D007854:Lead / Q000032:analysis; D008387:Marine Toxins; D053768:Metal Nanoparticles; D052998:Microcystins / Q000032:analysis; D009570:Nitriles / Q000032:analysis; D013739:Testosterone / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000728:Androgens; D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D001427:Bacterial Toxins; D060766:Drinking Water; D005659:Fungicides, Industrial; D008387:Marine Toxins; D052998:Microcystins; D009570:Nitriles; D014874:Water Pollutants, Chemical; D007854:Lead; D013739:Testosterone; D002701:Chloramphenicol; D006046:Gold; C057862:cyanoginosin LR; C005806:tetrachloroisophthalonitrile
keywords: Chloramphenicol; Gold nanoparticle immunochromatographic strip; Microcystin–leucine–arginine; Simultaneous detection; Testosterone