title: [Histochemical and immunohistochemical localization of choline acetyltransferase in the nuclei oblongata rat brain].
abstract: ChAT-positive neurons in the nuclei of the medulla oblongata of Wistar rats have been studied with the use of histochemical and immunohistochemical methods. We have found that the topography and the number of cholinergic neurons at the projection nuclei studied largely depend on the method of detection of these neurons. Histochemical method always revealed more neurons than immunohistochemistry. Such a feature of ChAT-positive neurons detection was clearly seen among the majority of nuclei in the medial region and in some of the nuclei in the lateral region of the medulla oblongata. The number of immunoreactive cells in the nuclei varied from 17 to 26%, whereas the histochemical reaction determined 1.5-3 times more neurons in the same nuclei. ChAT-positive cells in the nuclei of the back seam were detected mainly by a histochemical method.
authors.forename: A E
authors.initials: AE
authors.lastname: Kotsiuba
authors.forename: V M
authors.initials: VM
authors.lastname: Chertok
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D057075:Enzyme Assays; D007150:Immunohistochemistry; D008526:Medulla Oblongata / Q000201:enzymology / Q000648:ultrastructure*; D009474:Neurons / Q000737:chemistry* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000906:Antibodies; D002795:Choline O-Acetyltransferase