title: Efficacy of platelet-rich fibrin matrix on viability of diced cartilage grafts in a rabbit model.
abstract: OBJECTIVE
  The objective of this study was to compare the viability of cartilage grafts embedded in platelet-rich fibrin matrix (PRFM) wrapped with no material (bare diced cartilage grafts), oxidized methylcellulose (Surgicel), or acellular dermal tissue (AlloDerm).
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  In this study, six New Zealand rabbits were used. Cartilage grafts including perichondrium were excised from each ear and diced into 2-mm-by 2-mm pieces. There were four comparison groups: 1) group A, diced cartilage (not wrapped with any material); 2) group B, diced cartilage wrapped with AlloDerm; 3) group C, diced cartilage grafts wrapped with Surgicel; and 4) group D, diced cartilage wrapped with PRFM. Four cartilage grafts were implanted under the skin at the back of each rabbit. All rabbits were sacrificed at the end of 10 weeks. The cartilages were stained with hematoxylin-eosin, Masson's Trichrome, and Orcein. After that, they were evaluated for the viability of chondrocytes, collagen content, fibrillar structure of matrix, and changes in peripheral tissues.
  
  
  RESULTS
  When the viability of chondrocytes, the content of fiber in matrix, and changes in peripheral tissues were compared, the cartilage embedded in the PRFM group was statistically significantly higher than in the other groups (P < 0.05).
  
  
  CONCLUSIONS
  We concluded that PRFM has significant advantages in ensuring the chondrocyte viability of diced cartilage grafts. It is also biocompatible, with relatively lesser inflammation and fibrosis.
authors.affiliation: Department of Otolaryngology, School of Medicine, Ufuk University, Ankara, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Güler
authors.forename: Deniz
authors.initials: D
authors.lastname: Billur
authors.forename: Sevim
authors.initials: S
authors.lastname: Aydin
authors.forename: Sinan
authors.initials: S
authors.lastname: Kocatürk
mesh_terms: D000818:Animals; D001792:Blood Platelets; D002483:Cellulose, Oxidized / Q000494:pharmacology; D019902:Chondrocytes / Q000637:transplantation; D003094:Collagen / Q000494:pharmacology; D004195:Disease Models, Animal; D004425:Ear Cartilage / Q000637:transplantation*; D005337:Fibrin; D006085:Graft Survival; D019736:Prostheses and Implants; D011817:Rabbits; D012225:Rhinoplasty / Q000379:methods*
chemical_list: C440568:Alloderm; D002483:Cellulose, Oxidized; C013695:Surgicel; D005337:Fibrin; D003094:Collagen
keywords: AlloDerm; Platelet-rich fibrin matrix (PRFM); Surgicel; diced cartilage; rhinoplasty