title: Assessment of sensitivity and virulence fitness costs of the AvrPik alleles from Magnaporthe oryzae to isoprothiolane.
abstract: The in vitro sensitivity of AvrPik allele isolates of Magnaporthe oryzae to isoprothiolane was examined and the virulence fitness costs of AvrPik allele isolates to isoprothiolane were assessed. Isoprothiolane was found to suppress the radial growth of AvrPik allele isolates at all concentrations (1, 5, 10, 15, and 20 μg/mL). Generally, a higher isoprothiolane concentration has a stronger inhibitory effect on mycelial growth in AvrPik allele isolates at 6 and 10 days after inoculation. The inhibitory effect of isoprothiolane also increased with treatment time. To determine whether a correlation existed between the in vitro sensitivity of AvrPik allele isolates and virulence, the half-maximal inhibitor concentration and 75% of the maximum inhibitor concentration were calculated for each mutation isolate and wild-type isolate. Based on these values and virulence, no significant correlation between the susceptibility of AvrPik allele isolates and virulence was detected. In summary, no fitness costs were associated with sensitivity of blast isolates carrying specific AvrPik alleles to different virulence.
authors.affiliation: Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Guangdong Provincial Key Laboratory for Microbe Signals and Crop Disease Control, South China Agricultural University, Guangzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Liang
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Zheng
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China.
authors.forename: K X
authors.initials: KX
authors.lastname: Yi
authors.affiliation: Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China hechunppp@163.com.
authors.forename: C P
authors.initials: CP
authors.lastname: He
mesh_terms: D000483:Alleles; D004305:Dose-Response Relationship, Drug; D056084:Genetic Fitness / Q000187:drug effects*; D005838:Genotype; D020082:Magnaporthe / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D009154:Mutation; D025282:Mycelium / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D012275:Oryza / Q000382:microbiology; D010935:Plant Diseases / Q000382:microbiology; D013876:Thiophenes / Q000494:pharmacology*; D013997:Time Factors; D014774:Virulence
chemical_list: D013876:Thiophenes; C023889:isoprothiolane