title: Prophylactic treatment of asthma by an ozone scavenger in a mouse model.
abstract: Our hypothesis that inflammation in asthma involves production of ozone by white blood cells and that ozone could be an inflammatory mediator suggests that scavengers of reactive oxygen species (ROS), for example, electron-rich olefins, could serve for prophylactic treatment of asthma. Olefins could provide chemical protection against either exogenous or endogenous ozone and other ROS. BALB/c mice pretreated by inhalation of d-limonene before an ovalbumin challenge exhibited significant attenuation of the allergic asthma symptoms. Diminution of the inflammatory process was evident by reduced levels of aldehydes, reduced counts of neutrophils in the BAL fluid and by histological tests. A surprising systemic effect was observed by decreased levels of aldehydes in the spleen, suggesting that the examination of tissues and organs that are remote from the inflammation foci could provide valuable information on the distribution of the oxidative stress and may serve as guide for targeted treatment.
authors.affiliation: Department of Pediatrics, Barzilai Medical Center, Hahistadrout St. 2, Ashkelon 78278, Israel; Faculty of Medicine, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
authors.forename: Haim
authors.initials: H
authors.lastname: Bibi
authors.affiliation: Department of Natural Sciences, The Open University of Israel, 1 University Road, PO Box 808, Ra'anana 43537, Israel.
authors.forename: Ofer
authors.initials: O
authors.lastname: Reany
authors.affiliation: Department of Neonatology, Carmel Medical Center, Michal St. 7, Haifa 3436212, Israel; Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
authors.forename: Dan
authors.initials: D
authors.lastname: Waisman
authors.affiliation: Schulich Faculty of Chemistry, Technion-Israel Institute of Technology, Technion City, Haifa 32000, Israel. Electronic address: keinan@technion.ac.il.
authors.forename: Ehud
authors.initials: E
authors.lastname: Keinan
mesh_terms: D000280:Administration, Inhalation; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000494:pharmacology*; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D053138:Cyclohexenes / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D000077222:Limonene; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D015394:Molecular Structure; D010047:Ovalbumin / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010126:Ozone / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism; D013329:Structure-Activity Relationship; D013729:Terpenes / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000447:Aldehydes; D000894:Anti-Inflammatory Agents, Non-Steroidal; D053138:Cyclohexenes; D017382:Reactive Oxygen Species; D013729:Terpenes; D010126:Ozone; D010047:Ovalbumin; D000077222:Limonene
keywords: Aldehyde biomarkers; Asthma; Respiratory inflammation; Systemic oxidative stress; d-Limonene