title: Effect of cervus and cucumis peptides on osteoblast activity and fracture healing in osteoporotic bone.
abstract: Osteoporosis is associated with delayed and/or reduced fracture healing. As cervus and cucumis are the traditional Chinese treatments for rheumatoid arthritis, we investigated the effect of supplementation of these peptides (CCP) on bone fracture healing in ovariectomized (OVX) osteoporotic rats in vitro and in vivo. CCP enhanced osteoblast proliferation and increased alkaline phosphatase activity, matrix mineralization, and expression of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 4 (BMP4), and osteopontin. In vivo, female Sprague-Dawley rats underwent ovariectomy and the right femora were fractured and fixed by intramedullary nailing 3 months later. Rats received intraperitoneal injections of either CCP (1.67 mg/kg) or physiological saline every day for 30 days. Fracture healing and callus formation were evaluated by radiography, micro-CT, biomechanical testing, and histology. At 12 weeks after fracture, calluses in CCP-treated bones showed significantly higher torsional strength and greater stiffness than control-treated bones. Bones in CCP-treated rats reunified and were thoroughly remodeled, while two saline-treated rats showed no bone union and incomplete remodeling. Taken together, these results indicate that use of CCP after fracture in osteoporotic rats accelerates mineralization and osteogenesis and improves fracture healing.
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ai-Yuan
authors.initials: AY
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yuan
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Chen
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Xu
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hao-Ye
authors.initials: HY
authors.lastname: Meng
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Yu
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yao-Qin
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Quan-Yi
authors.initials: QY
authors.lastname: Guo
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shi-Bi
authors.initials: SB
authors.lastname: Lu
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Peng
authors.affiliation: Institute of Orthopedics, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang