title: Concordance study between the ParaDNA® Intelligence Test, a rapid DNA profiling assay, and a conventional STR typing kit (AmpFlSTR® SGM Plus®).
abstract: The ParaDNA® Intelligence Test enables STR profiling directly from human biological samples and evidence items collected from crime scene in 75min. Designed for non-expert use this system allows DNA information to be available to investigators before it would typically be available from a laboratory. The ParaDNA Intelligence Test system amplifies D3S1358, D8S119, D16S539, D18S1358 and TH01 STR loci and the gender typing locus amelogenin and detects the alleles present with HyBeacon® probes. Individual DNA samples from 381 UK Caucasian individuals were analysed using AmpFlSTR® SGM Plus® and the ParaDNA Intelligence Test with the derived STR profiles compared. Here we describe the high level of concordance demonstrated between the two systems and discuss this with reference to allele frequencies and the discriminatory power offered by the ParaDNA Intelligence Test.
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom. Electronic address: paradna@lgcgroup.com.
authors.forename: G
authors.initials: G
authors.lastname: Ball
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: N
authors.initials: N
authors.lastname: Dawnay
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: B
authors.initials: B
authors.lastname: Stafford-Allen
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Panasiuk
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Rendell
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Blackman
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: N
authors.initials: N
authors.lastname: Duxbury
authors.affiliation: LGC, ParaDNA Business Unit, F4 Culham Science Centre, Abingdon, Oxfordshire, OX14 3ED, United Kingdom.
authors.forename: S
authors.initials: S
authors.lastname: Wells
mesh_terms: D004247:DNA / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D053803:Forensic Genetics / Q000379:methods; D005787:Gene Frequency; D006801:Humans; D018895:Microsatellite Repeats; D011933:Reagent Kits, Diagnostic; D044465:White People / Q000235:genetics
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA
keywords: DNA typing; Direct PCR; ParaDNA; Rapid DNA; STR; Short tandem repeat