title: [Characteristics of functioning of succinate dehydrogenase from flight muscles of the bumblebee Bombus terrestris (L.)].
abstract: It was found that the succinate oxidation rate in mitochondria of flight muscles of Bombus terrestris L. in- creased by a factor of 2.15 after flying for 1 h. An electrophoretically homogenous preparation of succinate dehydrogenase with a specific activity of 7.14 U/mg protein and 81.55-fold purity was isolated from B. terrestris flight muscles. It is shown that this enzyme is represented in the muscle tissue by only one isoform with R,f = 0.24. The molecular weight of the native molecule and its subunits A and B was determined. The kinetic characteristics ofsuccinate dehydrogenase (Km = 0.33 mM) and the optimal concentration of hydrogen ions (pH 7.8) were established, and the effect of salts on the enzyme activity was studied. The role of succinate as a respiratory substrate in stress and the structural and functional characteristics of the succinate dehydrogenase system in the flight muscles of insects are discussed.
authors.forename: T M
authors.initials: TM
authors.lastname: Gorbacheva
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Syromiatnikov
authors.forename: V N
authors.initials: VN
authors.lastname: Popov
authors.forename: A V
authors.initials: AV
authors.lastname: Lopatin
authors.forename: A T
authors.initials: AT
authors.lastname: Eprintsev
authors.forename: D N
authors.initials: DN
authors.lastname: Fedorin
mesh_terms: D000818:Animals; D001516:Bees / Q000201:enzymology*; D005426:Flight, Animal; D008928:Mitochondria / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology*; D010084:Oxidation-Reduction; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
chemical_list: D019802:Succinic Acid; D013385:Succinate Dehydrogenase