title: Association of Hsp60 expression with damage to rat myocardial cells exposed to heat stress in vivo and in vitro.
abstract: To investigate the protective role of Hsp60 against stress damage and its role in the sudden death of stressed animals, changes in the levels of Hsp60 protein and hsp60 mRNA of myocardial cells in vivo and in vitro were studied. In addition, the relationship between Hsp60 expression and heat-induced damage was also studied. Rats were exposed to a temperature of 42° ± 1°C for 0, 20, 40, 60, 80, or 100 min. More than 50% of the rats died suddenly within 100 min. With increasing heat stress duration, hsp60 mRNA levels significantly increased in both in vivo and in vitro rat myocardial cells; however, a similar trend was not observed for Hsp60 protein levels. Although the changes observed in Hsp60 expression in myocardial cells in vitro were inconsistent with those of rat heart tissues in vivo, Hsp60 expression levels were consistent with the histopathological damage observed in myocardial cells both in vivo and in vitro. Differences in Hsp60 expression may reflect the degree of injury sustained by myocardial cells in vivo and in vitro. As a mitochondrial protein, Hsp60 represents a potential biomarker of heat stress, and may protect against heat stress induced myocardial cellular damage both in vivo and in vitro.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: J R
authors.initials: JR
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: H B
authors.initials: HB
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: A
authors.initials: A
authors.lastname: Abdelnasir
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: S
authors.initials: S
authors.lastname: Tang
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Kemper
authors.affiliation: Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Hartung
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China b_endong@njau.edu.cn.
authors.forename: E D
authors.initials: ED
authors.lastname: Bao
mesh_terms: D000818:Animals; D002460:Cell Line; D018834:Chaperonin 60 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D018869:Heat-Shock Response / Q000235:genetics; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic
chemical_list: D018834:Chaperonin 60; D012333:RNA, Messenger