title: Electroacupuncture ameliorates experimental colitis induced by TNBS through activation of interleukin-10 and inhibition of iNOS in mice.
abstract: OBJECTIVE
  To study the anti-inflammatory actions of electroacupuncture (EAc) on an experimental colitis model in mice.
  
  
  METHODS
  Thirty-eight male Swiss mice, divided in five groups, were subjected to induction of colitis by TNBS in 50% ethanol. Saline (SAL) and ethanol (ETNL) groups served as controls. TNBS+EAc and TNBS+ dexamethasone subgroups were treated with EAc 100Hz and dexamethasone (DEXA) 1 mg/Kg/day, respectively. After three days, a colon segment was obtained for quantification of myeloperoxidase (MPO) activity, immunohistochemistry for iNOS, malondialdehyde (MDA) and cytokines (IL-1β and IL-10).
  
  
  RESULTS
  Neutrophilic activity, assayed as MPO activity, was significantly higher in the TNBS colitis group than that in the saline control group. TNBS+EAc group showed suppression of IL-10 in the colon. EAc treatment significantly reduced the concentration of MDA and the expression of iNOS, as compared to the other groups.
  
  
  CONCLUSIONS
  Electroacupuncture 100Hz applied to acupoint ST-36 promotes an anti-inflammatory action on the TNBS-induced colitis, mediated by increase of IL-10 and decrease of iNOS expression.
authors.affiliation: Department of Surgery, Federal University of Ceara, Fortaleza, CE, Brazil.
authors.forename: Annya Costa Araujo de Macedo
authors.initials: AC
authors.lastname: Goes
authors.affiliation: School of Pharmacy, UFC, Fortaleza, CE, Brazil.
authors.forename: Francisco Maxwell Martins
authors.initials: FM
authors.lastname: Pinto
authors.affiliation: School of Pharmacy, UFC, Fortaleza, CE, Brazil.
authors.forename: Guilherme Cardoso
authors.initials: GC
authors.lastname: Fernandes
authors.affiliation: School of Medicine, UFC, Fortaleza, CE, Brazil.
authors.forename: Jessica Sales
authors.initials: JS
authors.lastname: Barbosa
authors.affiliation: Walter Cantidio Hospital, UFC, Fortaleza, CE, Brazil.
authors.forename: Emanuela Santos
authors.initials: ES
authors.lastname: Correia
authors.affiliation: Department of Surgery, UFC, Fortaleza, CE, Brazil.
authors.forename: Ronaldo Albuquerque
authors.initials: RA
authors.lastname: Ribeiro
authors.affiliation: UFC, Fortaleza, CE, Brazil.
authors.forename: Sergio Botelho
authors.initials: SB
authors.lastname: Guimaraes
authors.affiliation: Department of Pharmacology, UFC, Fortaleza, CE, Brazil.
authors.forename: Roberto Cesar Pereira
authors.initials: RC
authors.lastname: Lima Júnior
authors.affiliation: Department of Morphology, UFC, Fortaleza, CE, Brazil.
authors.forename: Gerly Anne de Castro
authors.initials: GA
authors.lastname: Brito
authors.affiliation: Department of Surgery, UFC, Fortaleza, CE, Brazil.
authors.forename: Lusmar Veras
authors.initials: LV
authors.lastname: Rodrigues
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000628:therapy*; D003106:Colon / Q000378:metabolism; D004195:Disease Models, Animal; D015671:Electroacupuncture / Q000379:methods*; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy / Q000628:therapy; D016753:Interleukin-10 / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism*; D011897:Random Allocation; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016753:Interleukin-10; D008315:Malondialdehyde; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D052247:Nitric Oxide Synthase Type II