title: Green light may improve diagnostic accuracy of nailfold capillaroscopy with a simple digital videomicroscope.
abstract: Nailfold capillaroscopy is a non-invasive and safe technique for the analysis of microangiopathologies. Imaging quality of widely used simple videomicroscopes is poor. The use of green illumination instead of the commonly used white light may improve contrast. The aim of the study was to compare the effect of green illumination with white illumination, regarding capillary density, the number of microangiopathologies, and sensitivity and specificity for systemic sclerosis. Five rheumatologists have evaluated 80 images; 40 images acquired with green light, and 40 images acquired with white light. A larger number of microangiopathologies were found in images acquired with green light than in images acquired with white light. This results in slightly higher sensitivity with green light in comparison with white light, without reducing the specificity. These findings suggest that green instead of white illumination may facilitate evaluation of capillaroscopic images obtained with a low-cost digital videomicroscope.
authors.affiliation: Faculty of Science and Technology, University of Twente, Enschede, The Netherlands.
authors.forename: Harm H A
authors.initials: HH
authors.lastname: Weekenstroo
authors.forename: Bart M W
authors.initials: BM
authors.lastname: Cornelissen
authors.forename: Hein J
authors.initials: HJ
authors.lastname: Bernelot Moens
mesh_terms: D002196:Capillaries / Q000473:pathology*; D003116:Color; D004867:Equipment Design; D006801:Humans; D008027:Light; D020678:Microscopic Angioscopy / Q000295:instrumentation*; D018715:Microscopy, Video / Q000295:instrumentation*; D009262:Nails / Q000098:blood supply*; D015588:Observer Variation; D011237:Predictive Value of Tests; D015203:Reproducibility of Results