title: Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998-2012.
abstract: Despite more than 50 years of vaccination, pertussis is still an endemic disease, with regular epidemic outbreaks. With the exception of Poland, European countries have replaced whole-cell vaccines (WCVs) by acellular vaccines (ACVs) in the 1990s. Worldwide, antigenic divergence in vaccine antigens has been found between vaccine strains and circulating strains. In this work, 466 Bordetella pertussis isolates collected in the period 1998-2012 from 13 European countries were characterised by multi-locus antigen sequence typing (MAST) of the pertussis toxin promoter (ptxP) and of the genes coding for proteins used in the ACVs: pertussis toxin (Ptx), pertactin (Prn), type 2 fimbriae (Fim2) and type 3 fimbriae (Fim3). Isolates were further characterised by fimbrial serotyping, multi-locus variable-number tandem repeat analysis (MLVA) and pulsed-field gel electrophoresis (PFGE). The results showed a very similar B. pertussis population for 12 countries using ACVs, while Poland, which uses a WCV, was quite distinct, suggesting that ACVs and WCVs select for different B. pertussis populations. This study forms a baseline for future studies on the effect of vaccination programmes on B. pertussis populations.
authors.affiliation: Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), P.O. Box 1, 3720 BA, Bilthoven, The Netherlands, marjolein.van.gent@rivm.nl.
authors.forename: M
authors.initials: M
authors.lastname: van Gent
authors.forename: C J
authors.initials: CJ
authors.lastname: Heuvelman
authors.forename: H G
authors.initials: HG
authors.lastname: van der Heide
authors.forename: H O
authors.initials: HO
authors.lastname: Hallander
authors.forename: A
authors.initials: A
authors.lastname: Advani
authors.forename: N
authors.initials: N
authors.lastname: Guiso
authors.forename: C H
authors.initials: CH
authors.lastname: Wirsing von Kőnig
authors.forename: D F
authors.initials: DF
authors.lastname: Vestrheim
authors.forename: T
authors.initials: T
authors.lastname: Dalby
authors.forename: N K
authors.initials: NK
authors.lastname: Fry
authors.forename: D
authors.initials: D
authors.lastname: Pierard
authors.forename: L
authors.initials: L
authors.lastname: Detemmerman
authors.forename: J
authors.initials: J
authors.lastname: Zavadilova
authors.forename: K
authors.initials: K
authors.lastname: Fabianova
authors.forename: C
authors.initials: C
authors.lastname: Logan
authors.forename: A
authors.initials: A
authors.lastname: Habington
authors.forename: M
authors.initials: M
authors.lastname: Byrne
authors.forename: A
authors.initials: A
authors.lastname: Lutyńska
authors.forename: E
authors.initials: E
authors.lastname: Mosiej
authors.forename: C
authors.initials: C
authors.lastname: Pelaz
authors.forename: K
authors.initials: K
authors.lastname: Gröndahl-Yli-Hannuksela
authors.forename: A M
authors.initials: AM
authors.lastname: Barkoff
authors.forename: J
authors.initials: J
authors.lastname: Mertsola
authors.forename: A
authors.initials: A
authors.lastname: Economopoulou
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.forename: F R
authors.initials: FR
authors.lastname: Mooi
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics; D001886:Bordetella pertussis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D005060:Europe / Q000453:epidemiology; D014644:Genetic Variation; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D037342:Pertussis Toxin / Q000235:genetics; D011401:Promoter Regions, Genetic; D012703:Serotyping; D014917:Whooping Cough / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D037342:Pertussis Toxin