title: [Development of transgenic chicken with a gene of human granulocyte colony-stimulating factor using sperm-mediated gene transfer].
abstract: Transgenic poultry with a gene of human granulocyte colony-stimulating factor (gcsf) was developed by arti- ficial insemination with the transfected sperm using the "Lipofectamin 2000" reagent. The ratio of trans- genic chickens carrying the human gcsf genome was 33.3% of the total number of birds obtained. It was shown that the concentration-of the human G-csfprotein in blood serum oftransgenic specimens varied from 50 to 220 pg/ml. The first generation of poultry was obtained as a result of insemination oftransgenic hens by sperm of anti-transgenic cocks, thereby inheritance of the foreign gene was found in 37.5% of the obtained chickens.
authors.forename: A V
authors.initials: AV
authors.lastname: Samoĭlov
authors.forename: A Z
authors.initials: AZ
authors.lastname: Kesian
authors.forename: N M
authors.initials: NM
authors.lastname: Suraeva
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002645:Chickens / Q000235:genetics*; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D008297:Male; D013094:Spermatozoa / Q000166:cytology
chemical_list: D016179:Granulocyte Colony-Stimulating Factor