title: BRCA1 promoter hypermethylation signature for early detection of breast cancer in the Vietnamese population.
abstract: Breast cancer, a leading cause of death among women in most countries worldwide, is rapidly increasing in incidence in Vietnam. One of biomarkers is the disruption of the genetic material including epigenetic changes like DNA methylation. With the aim of finding hypermethylation at CpG islands of promoter of BRCA1 gene, belonged to the tumor suppressor gene family, as the biomarker for breast cancer in Vietnamese population, sensitive methyl specific PCR (MSP) was carried out on 115 samples including 95 breast cancer specimens and 20 normal breast tissues with other diseases which were obtained from Ho Chi Minh City Medical Hospital, Vietnam. The result indicated that the frequency of BRCA1 hypermethylation reached 82.1% in the cases (p<0.001). In addition, the DNA hypermethylation of this candidate gene increased the possibility to be breast cancer with high incidence via calculated odd ratios (p<0.05). In conclusion, hypermethylation of this candidate gene could be used as the promising biomarker application with Vietnamese breast cancer patients.
authors.affiliation: Department of Biotechnology Applications in Medicine, Faculty of Biotechnology, Ho Chi Minh City Medicine and Pharmacy University, Vietnam E-mail : thuy.lha@ou.edu.vn.
authors.forename: Phuong Kim
authors.initials: PK
authors.lastname: Truong
authors.forename: Thuan Duc
authors.initials: TD
authors.lastname: Lao
authors.forename: Thao Phuong Thi
authors.initials: TP
authors.lastname: Doan
authors.forename: Thuy Ai Huyen
authors.initials: TA
authors.lastname: Le
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D055088:Early Detection of Cancer; D005260:Female; D005787:Gene Frequency; D006801:Humans; D011401:Promoter Regions, Genetic / Q000235:genetics*; D014744:Vietnam
chemical_list: D019313:BRCA1 Protein; C492913:BRCA1 protein, human; D014408:Biomarkers, Tumor