title: The serpin superfamily in channel catfish: identification, phylogenetic analysis and expression profiling in mucosal tissues after bacterial infections.
abstract: The superfamily of serine protease inhibitors (serpins) are broadly distributed in all kingdoms of life. Serpins play critical roles in an array of fundamental biological processes. In this study, we identified a complete set of 25 serpin genes from channel catfish genome by comprehensive data mining of existing genomic resources. Phylogenetic analysis verified their identities and supported the classification of serpins into six families as in mammals. Extensive comparative genomic analyses suggested that most serpins were conserved among vertebrates, while some were lineage-specific. Analysis of serpin gene expression in mucosal tissues after bacterial infections indicated that serpin genes were regulated in a tissue-specific and time-dependent manner. Distinct expression patterns between infections of the two pathogens were observed, indicating that much more rapid host responses of serpin expression were initiated after ESC infection than after columnaris infection. These studies set the foundation for future studies of host-pathogen interactions.
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Shikai
authors.initials: S
authors.lastname: Liu
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Zhenkui
authors.initials: Z
authors.lastname: Qin
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Lin
authors.initials: L
authors.lastname: Song
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Rex
authors.initials: R
authors.lastname: Dunham
authors.affiliation: The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences and Program of Cell and Molecular Biosciences, Auburn University, Auburn, AL 36849, USA. Electronic address: liuzhan@auburn.edu.
authors.forename: Zhanjiang
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D020610:Edwardsiella ictaluri / Q000276:immunology; D004756:Enterobacteriaceae Infections / Q000276:immunology* / Q000382:microbiology; D005393:Fish Diseases / Q000276:immunology / Q000382:microbiology; D045826:Flavobacteriaceae Infections / Q000276:immunology* / Q000382:microbiology; D005417:Flavobacterium / Q000276:immunology; D018628:Gene Dosage / Q000235:genetics; D015870:Gene Expression; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D007059:Ictaluridae / Q000276:immunology*; D009092:Mucous Membrane / Q000378:metabolism* / Q000382:microbiology; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D015843:Serpins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*
chemical_list: D015843:Serpins
keywords: Catfish; Expression; Genome; Infection; Mucosal immunity; Serpin