title: Inhibition of fibrin polymerization by synthetic peptides corresponding to Aα195-205 and γ69-77 sites of fibrin molecule.
abstract: Using the idea of "proline brackets" we have found four sites in fibrin amino acid sequence, and appropriate peptides were synthesized: γ69NPDESSKPN77, Bβ228QPDSSVKPY236, Bβ455RPFFPQ460 and Aα195LPSRDRQHLPL205. Turbidity and electron-microscopy analyses have demonstrated that synthetic peptide Aα195-205 specifically inhibited the stage of fibrin protofibril formation and peptide γ69-77 - the stage offibrin protofibril lateral association. The data obtained testify that there are the sites involved in these processes in the appropriate amino acid sequences of fibrin molecule.
authors.forename: T A
authors.initials: TA
authors.lastname: Pozniak
authors.forename: L P
authors.initials: LP
authors.lastname: Urvant
authors.forename: P G
authors.initials: PG
authors.lastname: Gritsenko
authors.forename: V I
authors.initials: VI
authors.lastname: Chernishov
authors.forename: N A
authors.initials: NA
authors.lastname: Pydiura
authors.forename: E V
authors.initials: EV
authors.lastname: Lugovskoi
authors.forename: S V
authors.initials: SV
authors.lastname: Komisarenko
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001777:Blood Coagulation / Q000187:drug effects; D005337:Fibrin / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry* / Q000494:pharmacology*; D058105:Polymerization; D011392:Proline / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry
chemical_list: D010446:Peptide Fragments; D005337:Fibrin; D005340:Fibrinogen; D011392:Proline; D013917:Thrombin