title: Improving the methods for isolation of monocyte and establishing macrophage cell culture in caprine model.
abstract: Monocytes are widely used for immunological research, especially in the study of innate immune system. Although methods for isolation of human monocytes have been established, the procedure for non-human monocyte has not been well developed. This paper describes an improved method for isolation of monocyte and the subsequent macrophage cultivation from caprine blood. Monocytes were isolated from 16 ml of heparinized caprine blood using double density methods; the Ficoll and Percoll. The number of monocytes obtained was 5.12 ± 0.89 × 10(7) cells/ml at 70 % purity. The isolated monocytes were maintained in 10 % fetal bovine serum-enriched Dulbecco's Modified Eagle Medium for maturation to form macrophage cell culture. At the end of the experiment, the harvested macrophage was 2.48 ± 0.33 × 10(6) cells/ml.
authors.affiliation: Faculty of Veterinary Medicine, Research Centre for Ruminant Diseases, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Nurrul Nasruddin
authors.initials: NN
authors.lastname: Shaqinah
authors.affiliation: Faculty of Veterinary Medicine, Research Centre for Ruminant Diseases, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Mazlan
authors.initials: M
authors.lastname: Mazlina
authors.affiliation: Faculty of Veterinary Medicine, Research Centre for Ruminant Diseases, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. mzamri@upm.edu.my.
authors.forename: M
authors.initials: M
authors.lastname: Zamri-Saad
authors.affiliation: Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
authors.forename: Hamzah
authors.initials: H
authors.lastname: Hazilawati
authors.affiliation: Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Kota Bahru, Kelantan, Malaysia.
authors.forename: Sabri
authors.initials: S
authors.lastname: Jasni
keywords: Caprine; Cell line; Isolation; Macrophage; Monocyte