title: Nanotentacle-structured magnetic particles for efficient capture of circulating tumor cells.
abstract: Circulating tumor cells (CTCs) have attracted considerable attention as promising markers for diagnosing and monitoring the cancer status. Despite many technological advances in isolating CTCs, the capture efficiency and purity still remain challenges that limit clinical practice. Here, the construction of "nanotentacle"-structured magnetic particles using M13-bacteriophage and their application for the efficient capturing of CTCs is demonstrated. The M13-bacteriophage to magnetic particles followed by modification with PEG is conjugated, and further tethered monoclonal antibodies against the epidermal receptor 2 (HER2). The use of nanotentacle-structured magnetic particles results in a high capture purity (>45%) and efficiency (>90%), even for a smaller number of cancer cells (≈25 cells) in whole blood. Furthermore, the cancer cells captured are shown to maintain a viability of greater than 84%. The approach can be effectively used for capturing CTCs with high efficiency and purity for the diagnosis and monitoring of cancer status.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Jo
authors.forename: Joong-jae
authors.initials: JJ
authors.lastname: Lee
authors.forename: Woosung
authors.initials: W
authors.lastname: Heu
authors.forename: Hak-Sung
authors.initials: HS
authors.lastname: Kim
mesh_terms: D000818:Animals; D017104:Bacteriophage M13 / Q000378:metabolism; D002452:Cell Count; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D061986:MCF-7 Cells; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism*; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000737:chemistry; D017422:Sequence Analysis, DNA; D000068878:Trastuzumab / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C508053:ERBB2 protein, human
keywords: M13-bacteriophage; cancer diagnosis; circulating tumor cells; enhanced binding avidity; immunomagnetic capture