title: Influence of dietary components on Aspergillus niger prolyl endoprotease mediated gluten degradation.
abstract: Celiac disease (CD) is caused by intolerance to gluten. Oral supplementation with enzymes like Aspergillus niger propyl-endoprotease (AN-PEP), which can hydrolyse gluten, has been proposed to prevent the harmful effects of ingestion of gluten. The influence of meal composition on AN-PEP activity was investigated using an in vitro model that simulates stomach-like conditions. AN-PEP optimal dosage was 20 proline protease units (PPU)/g gluten. The addition of a carbonated drink strongly enhanced AN-PEP activity because of its acidifying effect. While fat did not affect gluten degradation by AN-PEP, the presence of food proteins slowed down gluten detoxification. Moreover, raw gluten was degraded more efficiently by AN-PEP than baked gluten. We conclude that the meal composition influences the amount of AN-PEP needed for gluten elimination. Therefore, AN-PEP should not be used to replace a gluten free diet, but rather to support digestion of occasional and/or inadvertent gluten consumption.
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: vmonserratperez@gmail.com.
authors.forename: Veronica
authors.initials: V
authors.lastname: Montserrat
authors.affiliation: DSM Food Specialties, Delft, The Netherlands.
authors.forename: Maaike J
authors.initials: MJ
authors.lastname: Bruins
authors.affiliation: DSM Food Specialties, Delft, The Netherlands.
authors.forename: Luppo
authors.initials: L
authors.lastname: Edens
authors.affiliation: Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Frits
authors.initials: F
authors.lastname: Koning
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D002446:Celiac Disease / Q000628:therapy; D055050:Diet, Gluten-Free; D004041:Dietary Fats / Q000378:metabolism; D004044:Dietary Proteins / Q000378:metabolism; D004063:Digestion; D005983:Glutens / Q000378:metabolism*; D012697:Serine Endopeptidases / Q000378:metabolism*
chemical_list: D004041:Dietary Fats; D004044:Dietary Proteins; D005983:Glutens; D012697:Serine Endopeptidases
keywords: Diet; Endoprotease; Gluten; Oral supplementation