title: NMR assignments of actin depolymerizing factor (ADF) like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans.
abstract: The actin filament dynamics in nematode, Caenorhabditis elegans, is regulated by differential activity of two proteins UNC-60A and UNC-60B. UNC-60A exhibits strong pointed end depolymerization on C. elegans actin (Ce-actin), strong inhibition of polymerization, strong monomer sequestering activity, weak severing activity, and low affinity for F-actin binding, while UNC-60B exhibits strong pointed end depolymerization on rabbit muscle actin, strong severing activity, and high affinity for F-actin binding. Structural characterization of these proteins will help to understand (1) molecular mechanism of actin dynamics regulation and (2) the differential activity of these proteins. Here, we report (1)H, (13)C, and (15)N chemical shift assignments of these two proteins as determined by heteronuclear NMR experiments (at pH 6.5 and temperature 298 K).
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226 031, India.
authors.forename: Vaibhav Kumar
authors.initials: VK
authors.lastname: Shukla
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226 031, India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Kabra
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226 031, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Yadav
authors.affiliation: Department of Pathology, Emory University, Atlanta, GA, 30322, USA.|Department of Cell Biology, Emory University, Atlanta, GA, 30322, USA.
authors.forename: Shoichiro
authors.initials: S
authors.lastname: Ono
authors.affiliation: Centre of Biomedical Research, SGPGIMS Campus, R B Road, Lucknow, 226014, India.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, 226 031, India. ashishcdri@yahoo.com.
authors.forename: Ashish
authors.initials: A
authors.lastname: Arora
mesh_terms: D051339:Actin Depolymerizing Factors / Q000737:chemistry*; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D051345:Destrin / Q000737:chemistry*; D008840:Microfilament Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D051339:Actin Depolymerizing Factors; D029742:Caenorhabditis elegans Proteins; D051345:Destrin; D008840:Microfilament Proteins; C495209:UNC-60 protein, C elegans
keywords: ADF like protein; NMR; Resonance assignment; UNC-60A; UNC-60B