title: Identification of differentially expressed genes and small molecule drugs for the treatment of tendinopathy using microarray analysis.
abstract: Tendinopathy is a critical clinical problem as it is often asymptomatic at onset and during development, and is only recognized upon rupture of the tendon. It is common among recreational and competitive athletes. The present study sought to examine the molecular mechanism of the progression of tendinopathy by screening out differentially expressed genes (DEGs) and investigating their functions. In addition, the present study aimed to identify the small molecules, which exhibit potential effects, which could be utilized for the treatment of tendinopathy. The gene expression profile of tendinopathy, GSE26051 was downloaded from the Gene Expression Omnibus database, which included 23 control samples and 18 samples of tendinopathy. The DEGs were identified using the Limma package in the R programming language, and gene ontology and pathway enrichment analysis were performed. In addition, the potential regulatory microRNAs and the target sites of the transcription factors were screened out based on the molecular signature database. In addition, the DEGs were mapped to the connectivity map database to identify the potential small molecule drugs. A total of 318 genes were filtered as DEGs between diseased samples and normal control tendons. Additionally, genes, including laminin, α4, platelet‑derived growth factor α, laminin γ1 and Src homology 2 transforming protein 1 may induce tendinopathy through the focal adhesion pathway. Furthermore, the transcription factor, lymphoid enhancer‑binding factor 1 and its target genes, pantothenate kinase 2 and G protein‑coupled receptor kinase 5 were identified. The most significant microRNA, miR‑499, was screened and was found to regulate specific genes, including CUGBP2 and MYB. Additionally, the small molecules, Prestwick‑1082 and viomycin were identified to have the potential to repair disordered metabolic pathways and furthermore to remedy tendinopathy. The results of the present study assessed the mechanism of tendinopathy and screened small molecule drugs as potential treatments for this condition. In addition, the present findings have the potential for use in a clinical setting for the treatment of tendinopathy in the future.
authors.affiliation: Department of Orthopedics, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Shanghai 10th People's Hospital, Tongji University, Shanghai 200072, P.R. China.
authors.forename: Lieming
authors.initials: L
authors.lastname: Lou
mesh_terms: D001665:Binding Sites; D019295:Computational Biology; D030561:Databases, Nucleic Acid; D066264:Datasets as Topic; D055808:Drug Discovery; D004353:Drug Evaluation, Preclinical; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D011485:Protein Binding; D015398:Signal Transduction; D054852:Small Molecule Libraries; D052256:Tendinopathy / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D054852:Small Molecule Libraries; D014157:Transcription Factors