title: [Anti-gastric cancer effect of melatonin and Bcl-2, Bax, p21 and p53 expression changes].
abstract: In order to investigate the role of melatonin in inhibiting the proliferation of murine gastric cancer and the underlying molecular mechanism, we performed an in vivo study by inoculating murine foregastric carcinoma (MFC) cells in mice, and then tumor-bearing mice were treated with different concentrations of melatonin (i.p.). The changes of Bcl-2, Bax, p21 and p53 expressions in tumor tissue were detected by using real-time fluorescence quantitative RT-PCR and Western blot. We found that: (1) melatonin resulted in reductions of tumor's volume and weight in the gastric cancer-bearing mice and thus showed anti-cancer effect; (2) melatonin reduced Bcl-2 expression, but increased the expression of Bax, p53 and p21 in tumor tissue. Our results suggest that melatonin could inhibit the growth of tumors in gastric cancer-bearing mice through accelerating the apoptosis of tumor cells.
authors.affiliation: Department of Physiology, Basic Medical College of Putian University, Putian 351100, China; Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University, Fuzhou 350004, China. zhourx@mail.fjmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Qing-Dong
authors.initials: QD
authors.lastname: Jin
authors.forename: Xi
authors.initials: X
authors.lastname: Gong
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Rui-Xiang
authors.initials: RX
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis; D008550:Melatonin / Q000494:pharmacology*; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494827:Bax protein, mouse; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; C052617:Bcl2 protein, mouse; D008550:Melatonin