title: Evaluation of the MALDI-TOF MS profiling for identification of newly described Aeromonas spp.
abstract: The genus Aeromonas comprises primarily aquatic bacteria and also serious human and animal pathogens with the occurrence in clinical material, drinking water, and food. Aeromonads are typical for their complex taxonomy and nomenclature and for limited possibilities of identification to the species level. According to studies describing the use of MALDI-TOF MS in diagnostics of aeromonads, this modern chemotaxonomical approach reveals quite high percentage of correctly identified isolates. We analyzed 64 Aeromonas reference strains from the set of 27 species. After extending the range of analyzed Aeromonas species by newly described ones, we proved that MALDI-TOF MS procedure accompanied by Biotyper tool is not a reliable diagnostic technique for aeromonads. We obtained quite high percentage of false-positive, incorrect, and uncertain results. The identification of newly described species is accompanied with misidentifications that were observed also in the case of pathogenic aeromonads.
authors.affiliation: Division of Microbiology, Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 735/5, 625 00, Brno, Czech Republic, ajkavav@gmail.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vávrová
authors.forename: Tereza
authors.initials: T
authors.lastname: Balážová
authors.forename: Ivo
authors.initials: I
authors.lastname: Sedláček
authors.forename: Ludmila
authors.initials: L
authors.lastname: Tvrzová
authors.forename: Ondrej
authors.initials: O
authors.lastname: Šedo
mesh_terms: D000333:Aeromonas / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification*; D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D004783:Environmental Microbiology; D005516:Food Microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology* / Q000662:veterinary*; D006801:Humans; D010802:Phylogeny; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*