title: [Comparative antioxidant action on the level of reactive oxygen species in normal and transformed fibroblasts].
abstract: We studied the changes in the level of reactive oxygen species (ROS) in normal (3T3) and transformed (3T3-SV40) murine fibroblasts under the antioxidant action for 15 min using fluorescent probe carbo- xy-H2DCFDA. We have shown that N-acetylcysteine (NAC) decreased ROS level in both cellular types. Another antioxidant, alpha-lipoic acid (ALA), and its reduced form, dihydrolipoic acid (DHLA), caused the prooxidant effects. Both ALA and DHLA in the concentration range of 0.1-1.25 mM increased ROS level in dose dependent manner in both cellular types. The ability of ALA and DHLA to activate hydrogen peroxide production is discussed.
authors.forename: O G
authors.initials: OG
authors.lastname: Liublinskaia
authors.forename: K M
authors.initials: KM
authors.lastname: Kirpichnikova
authors.forename: I A
authors.initials: IA
authors.lastname: Gamaleĭ
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002461:Cell Line, Transformed; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D041681:NIH 3T3 Cells; D017382:Reactive Oxygen Species / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D005452:Fluoresceins; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D008063:Thioctic Acid; C015144:dihydrolipoic acid; D006861:Hydrogen Peroxide; D000111:Acetylcysteine