title: [Growth inhibition effection of perlecan anti-sense cDNA on human laryngeal carcinoma xnograft in nude mice].
abstract: OBJECTIVE
  To observe growth inhibition effect of perlecan anti-sense cDNA (pAP) on human laryngeal carcinoma xnografted in nude mice. To vertify its antitumor effect and mechanism in vivo, and it may be useful as a biomarker in carcinoma of larynx cancer.
  
  
  METHODS
  Created the model of human laryngeal carcinoma xnograft in nude mice. To observe growth of those xnografts in nude mice and draw growth curve of xnografted. The expression of perlecan mRNA and portein in xnografts were examined by RT-PCR and immunohistochemistry.
  
  
  RESULTS
  Volume of xnografts in the group transfected by the plasmids of pAP were significant small as compared with other two groups made by the wild type cells and phpApr-neol cells (P < 0.05). It was showed that the expression of perlecan mRNA and protein were significantly reduced in the tumor of pAP transfected Hep-2 cells as compared with the tumors transfected by the wild type cells and phβApr-neol cells (P < 0.01).
  
  
  CONCLUSIONS
  These data raise the possibility that pAP many play key roles in the growth of those xnografts in nude mice.
authors.forename: Guangli
authors.initials: G
authors.lastname: Chen
authors.forename: Shusheng
authors.initials: S
authors.lastname: Gong
authors.forename: Pei
authors.initials: P
authors.lastname: Chen
authors.forename: Linghui
authors.initials: L
authors.lastname: Luo
mesh_terms: D000818:Animals; D016373:DNA, Antisense / Q000627:therapeutic use*; D018076:DNA, Complementary; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics*; D064593:Heterografts; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D016373:DNA, Antisense; D018076:DNA, Complementary; D019812:Heparan Sulfate Proteoglycans; D012333:RNA, Messenger; C071927:perlecan