title: Effect of long-after glow phosphorescent pigment on reproductive parameters and ovarian maturation in the yellowtail damselfish, Chrysiptera parasema.
abstract: Photoperiod is considered the most important factor that entrains animal rhythms, including the reproductive cycle. The present study tested differences in sex maturation and sex steroid hormones of yellowtail damselfish (Chrysiptera parasema) exposed to a white fluorescent bulb (12L:12D and 14L:10D) or long-afterglow phosphorescent pigment (LumiNova sheet) for 4 months. At the end of the experiment, in the phosphorescent group, mRNA expressions of gonadotropin hormones [(GTHs, including gonadotropin (GTH) α and luteinizing hormone (LH) β)], estrogen receptor (ER), and vitellogenin were significantly higher than in the photoperiod groups (12L:12D and 14L:10D), and these results are consistent with those of Western blotting for protein expression. Furthermore, in the phosphorescent group, plasma FSH, LH, and estradiol-17β (E2) levels were significantly higher than in the photoperiod groups. However, plasma melatonin levels were significantly lower than in the photoperiod groups. Because LumiNova sheets continue to emit green light (520 nm) for approximately 2h after sunset, the extended light conditions probably contributed to reproductive ability in the experimental fish. In conclusion, long-afterglow phosphorescent pigment can be used for energy-efficient aquaculture to regulate the reproduction of fish, although its effect needs to be evaluated in other species.
authors.affiliation: Division of Marine BioScience, Korea Maritime and Ocean University, Busan 606-791, Republic of Korea.
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Future Aquaculture Research Institute, NFRDI, Jeju 699-804, Republic of Korea.
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Division of Marine BioScience, Korea Maritime and Ocean University, Busan 606-791, Republic of Korea. Electronic address: choic@kmou.ac.kr.
authors.forename: Cheol Young
authors.initials: CY
authors.lastname: Choi
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D018507:Gene Expression Regulation, Developmental; D006062:Gonadotropins / Q000235:genetics; D049408:Luminescent Agents / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000097:blood / Q000235:genetics; D008297:Male; D008550:Melatonin / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D010473:Perciformes / Q000502:physiology*; D017440:Photoperiod; D011960:Receptors, Estrogen / Q000235:genetics; D012098:Reproduction / Q000187:drug effects / Q000502:physiology; D012741:Sexual Maturation; D014819:Vitellogenins / Q000235:genetics
chemical_list: D006062:Gonadotropins; D049408:Luminescent Agents; D011960:Receptors, Estrogen; D014819:Vitellogenins; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D008550:Melatonin
keywords: Long-afterglow; Phosphorescent; Sex maturation; Yellowtail damselfish