title: Anti-diabetic activity of different oils through their effect on arylsulfatases.
abstract: BACKGROUND
  Diabetes mellitus (DM) is characterized by the overproduction of the reactive oxygen species which affects the integrity of the lysosomal membrane affecting lysosomal enzymes. The effect of these species is blocked by some natural products as antioxidants. In the current study, groups of normal and streptozotocin (STZ)-induced diabetic rats were treated by Nigella sativa (NS), olive and canola oils and subjected to the study of arylsulfatases as a model of lysosomal enzymes. The aim of the present study is to investigate the effect of STZ-induced diabetes on arylsulfatases in presence and absence of NS, olive and canola oils.
  
  
  METHODS
  Different groups of rats were induced by STZ, treated with different oils and compared to their corresponding control group. All groups were subjected for the assays of blood glucose, insulin, catalase and arylsulfatases. A comparative kinetic study of arylsulfatses was performed to detect the alteration of catalytic characterization.
  
  
  RESULTS
  The results demonstrated that diabetes causes a significant elevation in the level of hepatic arylsulfatase B and a significant reduction of hepatic catalase as an antioxidant enzyme. NS and olive oils returned catalase and arylsulfatase B activities back near to normal by fixing their catalytic properties. Furthermore, the maximum velocity of arylsulfatases A and B was significantly elevated in the induced diabetes, whereas their Km values were significantly changed. The treatment of diabetic rats by NS and olive oils reduced the degree of significance.
  
  
  CONCLUSIONS
  Diabetes induces significant alterations of the catalytic characters of arylsulfatases and some oils decrease this alteration through an antioxidant-mediated effect.
authors.affiliation: Department of Biological & Environmental Sciences, Faculty of Science, Beirut Arab University, Beirut, Lebanon.
authors.forename: Rima
authors.initials: R
authors.lastname: Samarji
authors.affiliation: Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, Egypt.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Balbaa
keywords: Arylsulfatases; Catalase; Diabetes; Kinetic parameters; Oxidative stress