title: Methylation of ras association domain protein 10 (RASSF10) promoter negative association with the survival of gastric cancer.
abstract: OBJECTIVE
  The present study was conducted to elucidate the prognostic prediction value of the methylation of the RASSF10 promoter in gastric cancer (GC).
  
  
  METHODS
  A total of 300 patients with GC revealed the methylation degrees of the DNA of the RASSF10 promoter. Methylation-specific PCR (MSP) analysis was performed to qualitatively detect the methylated degrees of the DNA of the RASSF10 promoter of 300 patients with GC. Associations between molecular, clinicopathological and survival data were analyzed.
  
  
  RESULTS
  The protein and mRNA expressions of RASSF10 in GC tissues were lower than those in normal gastric mucosal tissues. In the MSP analysis cohort, patients with methylated RASSF10 promoter exhibited significantly shorter median OS than those with unmethylated RASSF10 promoter (P < 0.001). Multivariate survival analysis results showed that methylated RASSF10 promoter was an independent predictor of the survival of patients with GC.
  
  
  CONCLUSIONS
  The methylation of the RASSF10 promoter could be applied for the clinical prediction of the prognosis of GC.
authors.affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Deng
authors.affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liang
authors.affiliation: Central Laboratory, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Guoguang
authors.initials: G
authors.lastname: Ying
authors.affiliation: Department of Epidemiology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Haixin
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Xingming
authors.initials: X
authors.lastname: Xie
authors.affiliation: Institute of Digestive Disease, Li Ka Shing Institute of Health Science, Chinese University of Hong Kong Shatin, Hong Kong.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University Xi'an, China.
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Tianjin Medical University Cancer Hospital, City Key Laboratory of Tianjin Cancer Center and National Clinical Research Center for Cancer Tianjin, China.
authors.forename: Xishan
authors.initials: X
authors.lastname: Hao
keywords: Stomach; methylation; neoplasm; ras association domain protein; survival