title: [Characteristics of primary cell culture from neonatal thyroid grand of pigs: folliculogenesis, hormone and growth].
abstract: In the study, comprehensive assessment of proliferative and hormonal activity of primary cell cultures derived from neonatal pig thyroid has been carried out for the first time. We have evaluated the basal and TSH-stimulated secretion of thyroxine and morphological features of culture, depending on the initial state of the material placed in culture: in the form of single cells or follicular conglomerates. Folliculogenesis and formation of dome structures were observed in culture spontaneous and under chronic TSH stimulation. The ability of the cells to expression of β-III-tubulin during prolonged cultivation in the presence of NGF has been demonstrated in the study.
authors.forename: S B
authors.initials: SB
authors.lastname: Biliavskaia
authors.forename: G A
authors.initials: GA
authors.lastname: Bozhok
authors.forename: E I
authors.initials: EI
authors.lastname: Legach
authors.forename: I A
authors.initials: IA
authors.lastname: Borovoĭ
authors.forename: I M
authors.initials: IM
authors.lastname: Gella
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Maliukin
authors.forename: T P
authors.initials: TP
authors.lastname: Bondarenko
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015870:Gene Expression; D020932:Nerve Growth Factor / Q000494:pharmacology; D061251:Primary Cell Culture; D013552:Swine; D013961:Thyroid Gland / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013972:Thyrotropin / Q000494:pharmacology; D013974:Thyroxine / Q000096:biosynthesis* / Q000378:metabolism; D014404:Tubulin / Q000235:genetics / Q000378:metabolism
chemical_list: D014404:Tubulin; D013972:Thyrotropin; D020932:Nerve Growth Factor; D013974:Thyroxine