title: The hamster (Mesocricetus auratus) as an experimental model of toxocariasis: histopathological, immunohistochemical, and immunoelectron microscopic findings.
abstract: Toxocariasis is a globally distributed parasitic infection caused by the larval stage of Toxocara spp. The typical natural hosts of the parasite are dogs and cats, but humans can be infected by the larval stage of the parasite after ingesting embryonated eggs in soil or from contaminated hands or fomites. The migrating larvae are not adapted to complete their life cycle within accidental or paratenic hosts like humans and laboratory animals, respectively, but they are capable of invading viscera or other tissues where they may survive and induce disease. In order to characterize hamsters (Mesocricetus auratus) as a model for Toxocara canis infection, histopathological and immunohistochemistry procedures were used to detect pathological lesions and the distribution of toxocaral antigens in the liver, lungs, and kidneys of experimentally infected animals. We also attempted to characterize the immunological parameters of the inflammatory response and correlate them with the histopathological findings. In the kidney, a correlation between glomerular changes and antigen deposits was evaluated using immunoelectron microscopy. The hamster is an adequate model of experimental toxocariasis for short-term investigations and has a good immunological and pathological response to the infection. Lung and liver manifestations of toxocariasis in hamsters approximated those in humans and other experimental animal models. A mixed Th2 immunological response to T. canis infection was predominant. The hamster model displayed a progressive rise of anti-toxocaral antibodies with the formation of immune complexes. Circulating antigens, immunoglobulin, and complement deposits were detected in the kidney without the development of a definite immune complex nephropathy.
authors.affiliation: Institute of Tropical Medicine, University of São Paulo, São Paulo, SP, Brazil, amgs@usp.br.
authors.forename: Ana Maria Gonçalves
authors.initials: AM
authors.lastname: da Silva
authors.forename: Pedro Paulo
authors.initials: PP
authors.lastname: Chieffi
authors.forename: Wellington Luiz Ferreira
authors.initials: WL
authors.lastname: da Silva
authors.forename: Edite Hatsumi Yamashiro
authors.initials: EH
authors.lastname: Kanashiro
authors.forename: Guita
authors.initials: G
authors.lastname: Rubinsky-Elefant
authors.forename: Edécio
authors.initials: E
authors.lastname: Cunha-Neto
authors.forename: Eliane Conti
authors.initials: EC
authors.lastname: Mairena
authors.forename: Thales
authors.initials: T
authors.lastname: De Brito
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis; D006224:Cricetinae; D004195:Disease Models, Animal; D007136:Immunoglobulins / Q000032:analysis; D007150:Immunohistochemistry; D007668:Kidney / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D007814:Larva / Q000254:growth & development; D008018:Life Cycle Stages; D008099:Liver / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008168:Lung / Q000276:immunology / Q000469:parasitology / Q000473:pathology; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D014119:Toxocara / Q000276:immunology / Q000469:parasitology*; D014120:Toxocariasis / Q000276:immunology / Q000469:parasitology* / Q000473:pathology*
chemical_list: D000947:Antigens, Helminth; D007136:Immunoglobulins; D003165:Complement System Proteins