title: Evaluation of co-solvent fraction, pressure and temperature effects in analytical and preparative supercritical fluid chromatography.
abstract: A chemometric approach is used for studying the combined effect of temperature, pressure and co-solvent fraction in analytical and preparative supercritical fluid chromatography (SFC). More specifically, by utilizing design of experiments coupled with careful measurements of the experimental conditions the interaction between pressure, temperature and co-solvent fraction was studied with respect to productivity, selectivity and retention in chiral SFC. A tris-(3,5-dimethylphenyl) carbamoyl cellulose stationary phase with carbon dioxide/methanol as mobile phase and the two racemic analytes trans-stilbene oxide (TSO) and 1,1′-bi-2-naphthol (BINOL) were investigated. It was found for the investigated model system that the co-solvent fraction and pressure were the parameters that most affected the retention factors and that the co-solvent fraction and column temperature were most important for controlling the selectivity. The productivity in the preparative mode of SFC was most influenced by the co-solvent fraction and temperature. Both high co-solvent fraction and temperature gave maximum productivity in the studied design space.
authors.affiliation: INTERACT, Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden.
authors.forename: Dennis
authors.initials: D
authors.lastname: Åsberg
authors.affiliation: INTERACT, Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Enmark
authors.affiliation: INTERACT, Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden. Electronic address: Jorgen.Samuelsson@kau.se.
authors.forename: Jörgen
authors.initials: J
authors.lastname: Samuelsson
authors.affiliation: INTERACT, Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden. Electronic address: Torgny.Fornstedt@kau.se.
authors.forename: Torgny
authors.initials: T
authors.lastname: Fornstedt
mesh_terms: D002245:Carbon Dioxide / Q000737:chemistry; D025924:Chromatography, Supercritical Fluid / Q000295:instrumentation / Q000379:methods*; D000432:Methanol / Q000737:chemistry; D009284:Naphthols / Q000737:chemistry; D011312:Pressure; D012997:Solvents / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry; D013696:Temperature
chemical_list: D009284:Naphthols; D012997:Solvents; D013267:Stilbenes; D002245:Carbon Dioxide; C412332:1,1'-bi-2-naphthol; C025906:stilbene oxide; D000432:Methanol