title: Diagnosis of human metapneumovirus in patients hospitalized with acute lower respiratory tract infection using a metal-enhanced fluorescence technique.
abstract: Human metapneumovirus (hMPV) is a common respiratory tract infection in children. However, conventional immunofluorescence assays (IFAs) for detecting hMPV in respiratory samples have limited reliability with a sensitivity and false-negative predictive value of 58.1% and approximately 17.8%, respectively. In this study, hMPV was measured in 91 clinical respiratory samples (55 sputum and 36 nasopharyngeal aspirate samples), which were obtained from children under three years of age, utilizing our previously developed high-throughput metal-enhanced fluorescence (MEF)-based biosensor (HT-MEFB). The sensitivity of HT-MEFB for hMPV detection in the 91 samples was improved by up to 77.4% compared with that obtained with IFAs, and the specificity of HT-MEFB for hMPV detection was 91.7%. In addition, the specificity and accuracy obtained after the selection of 55 sputum samples as the analyzed specimen reached 92.3% and 90.9%, respectively. Thus, in terms of accuracy, high throughput, and sensitivity, HT-MEFB exhibits considerable potential for hMPV detection in clinical settings.
authors.affiliation: Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan; National Taiwan University, Department of Biochemical Science and Technology, Taipei 10617, Taiwan.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Chang
authors.affiliation: Linkou Chang Gung Memorial Hospital, Department of Laboratory Medicine, Taoyuan 33305, Taiwan; Chang Gung University, College of Medicine, Department of Medical Biotechnology and Laboratory Science, Taoyuan 33371, Taiwan.
authors.forename: Kuo-Chien
authors.initials: KC
authors.lastname: Tsao
authors.affiliation: Linkou Chang Gung Memorial Hospital, Department of Laboratory Medicine, Taoyuan 33305, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan.
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Yu
authors.affiliation: Linkou Chang Gung Memorial Hospital, Division of Pediatric Infectious Diseases, Taoyuan 33305, Taiwan; Chang Gung University, College of Medicine, Department of Medicine, Taoyuan 33371, Taiwan.
authors.forename: Yhu-Chering
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Chang Gung University, Graduate Institute of Electro-Optical Engineering, Taoyuan 33371, Taiwan; Chang Gung University, Healthy Aging Research Center, Taoyuan 33302, Taiwan. Electronic address: cchou01@gamil.com.
authors.forename: Chien
authors.initials: C
authors.lastname: Chou
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D029121:Metapneumovirus / Q000302:isolation & purification*; D009305:Nasopharynx / Q000821:virology; D018184:Paramyxoviridae Infections / Q000175:diagnosis* / Q000821:virology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000821:virology; D012680:Sensitivity and Specificity; D013183:Sputum / Q000821:virology
keywords: Gold nanoparticles; High-throughput biosensor; Localized surface plasmon resonance; Metal-enhanced fluorescence; Virus detection