title: Characterization of three putative xylulose 5-phosphate/fructose 6-phosphate phosphoketolases in the cyanobacterium Anabaena sp. PCC 7120.
abstract: Xylulose 5-phosphate/fructose 6-phosphate phosphoketolase (Xfp) is a key enzyme in the central carbohydrate metabolism in heterofermentative bacteria, in which enzymatic property of Xfps is well characterized. This is not the case in other microbes. The cyanobacterium Anabaena sp. PCC 7120 possesses three putative genes encoding Xfp, all1483, all2567, and alr1850. We purified three putative Xfps as recombinant proteins. The results of gel filtration indicated that these proteins form homomultimer complex. All1483 and All2567 showed phosphoketolase activity, whereas Alr1850 did not show the activity. Kinetic analyses demonstrated that substrates, fructose 6-phosphate and inorganic phosphate, are cooperatively bound to enzymes positively and negatively, respectively.
authors.affiliation: a Department of Life Sciences, Graduate School of Arts and Sciences , The University of Tokyo , Meguro-ku , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Moriyama
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Tajima
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Sekine
authors.forename: Naoki
authors.initials: N
authors.lastname: Sato
mesh_terms: D000446:Aldehyde-Lyases / Q000235:genetics / Q000378:metabolism*; D017033:Anabaena / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000235:genetics; D005636:Fructosephosphates / Q000378:metabolism; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005636:Fructosephosphates; D008670:Metals; D011994:Recombinant Proteins; C027618:fructose-6-phosphate; D000446:Aldehyde-Lyases; C065556:fructose-6-phosphate phosphoketolase; C022367:phosphoketolase
keywords: Xfp; cooperative binding; cyanobacteria; phosphoketolase