title: An optimal polymerization process for low mean molecular weight HBOC with lower dimer.
abstract: The new research tried to improve the distribution of molecular weight of Hb-based oxygen carriers (HBOC), a bottleneck of glutaraldehyde (GDA)-polymerization process. The orthogonal experiments were done on the basis of the early study of human placenta Hemoglobin (Hb)-crosslinked-GDA and three factors were selected including the molar ratio of GDA and Hb, Hb concentration, and the rate of the feeding GDA. The optimal match condition of polymerization process prepared for the purpose of lower mean molecular weight, content of super-weight molecule, and the content of dimer. The results showed that the molar ratio of GDA and Hb was the greatest influencing factor on the molecular weight distribution of polymerized-Hb, followed by the Hb concentration, and the last is the rate of feeding GDA. The optimum matching conditions had reached the objective that the mean molecular weight with 155.54 ± 5.79, the content of dimer with 17.23 ± 3.71, and content of super-weight molecule with 0.17 ± 0.09, and the results can be repeated in the 30 times expansion experiments.
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences , Chengdu, Sichuan , P. R. China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Zhou
authors.forename: Shen
authors.initials: S
authors.lastname: Li
authors.forename: Shasha
authors.initials: S
authors.lastname: Hao
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Liu
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Chengmin
authors.initials: C
authors.lastname: Yang
mesh_terms: D001802:Blood Substitutes / Q000737:chemistry*; D005260:Female; D005976:Glutaral / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008970:Molecular Weight; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D055503:Protein Multimerization
chemical_list: D001802:Blood Substitutes; D006454:Hemoglobins; D005976:Glutaral
keywords: Hb concentration; human placenta Hb; molar ratio of GDA and Hb; orthogonal experiment; rate of feeding GDA