title: The phospholipidomic signatures of human blood microparticles, platelets and platelet-derived microparticles: a comparative HILIC-ESI-MS investigation.
abstract: The phospholipidomic signatures of human blood microparticles and platelets, evaluated by hydrophilic interaction liquid chromatography coupled to electrospray ionization--mass spectrometry, were compared. The phospholipidome of platelet-derived microparticles, obtained by platelets stimulation with a mixture of Ca(II), thrombin and collagen, was also considered for the comparison. Platelets, blood microparticles and platelet-derived microparticles displayed qualitatively similar phospholipidomes, all based on eight major phospholipid classes, namely: phosphatidylcholines, diacyl- and plasme(a)nyl-phosphatidylethanolamines, phosphatidylserines, phosphatidylinositols, sphingomyelins and lyso forms of phosphatidylcholines and phosphatidylethanolamines. However, while the phospholipidomes of platelets and platelet-derived microparticles were found to be generally similar also from a quantitative point of view, a higher relative incidence of species bearing polyunsaturated side chains, especially in phospholipid classes sharing the choline head (i.e. phosphatidylcholines and lyso-phosphatidylcholines), was observed in the case of blood microparticles. As a further peculiar feature, never reported before, the relative abundance of lyso-phosphatidylcholines among the eight identified phospholipid classes was found to be significantly higher in the lipid extracts of blood microparticles.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via E. Orabona 4, 70126, Bari, Italy, ilario.losito@uniba.it.
authors.forename: Ilario
authors.initials: I
authors.lastname: Losito
authors.forename: Elena
authors.initials: E
authors.lastname: Conte
authors.forename: Tommaso R I
authors.initials: TR
authors.lastname: Cataldi
authors.forename: Nicola
authors.initials: N
authors.lastname: Cioffi
authors.forename: Francesco M
authors.initials: FM
authors.lastname: Megli
authors.forename: Francesco
authors.initials: F
authors.lastname: Palmisano
mesh_terms: D001792:Blood Platelets / Q000737:chemistry*; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D002853:Chromatography, Liquid; D006801:Humans; D010743:Phospholipids / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010743:Phospholipids