title: Detection of thrombin with an aptamer-based macromolecule biosensor using bacterial ghost system.
abstract: A rapid on-site detection of exogenous proteins without the need for equipped laboratories or skilled personnel would benefit many areas. We built a rapid protein detection platform based on aptamer-induced inner-membrane scaffolds dimerization by virtue of bacterial ghost system. When the detection platform was coincubated with two kinds of aptamers targeting two different sites of thrombin, green fluorescence or β-lactamase activity were yielded with two different designs. The latter was detected by commercially available testing strips.
authors.affiliation: Zhejiang University Team (ZJU-China) for the International Genetically Engineered Machine Competition (iGEM), ‡College of Life Sciences, §School of Medicine, ∥Department of Chemical and Biological Engineering, ⊥College of Agriculture and Biotechnology, #Department of Chemistry, Zhejiang University , Hangzhou 310058, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Ding
authors.forename: Yujie
authors.initials: Y
authors.lastname: Chen
authors.forename: Lifeng
authors.initials: L
authors.lastname: Zhang
authors.forename: Zukai
authors.initials: Z
authors.lastname: Liu
authors.forename: Angli
authors.initials: A
authors.lastname: Xue
authors.forename: Wenjia
authors.initials: W
authors.lastname: Gu
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Yang
authors.forename: Xihan
authors.initials: X
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Congcong
authors.initials: C
authors.lastname: Xing
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Cao
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
mesh_terms: D052157:Aptamers, Nucleotide / Q000235:genetics*; D015374:Biosensing Techniques / Q000379:methods*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin