title: Simultaneous Determination of 10 Bioactive Components of Lophatherum gracile Brongn by HPLC-DAD.
abstract: A high-performance liquid chromatography method coupled with diode array detection (HPLC-DAD) was developed for simultaneous determination of two coumarins and eight flavonoids in Lophatherum gracile Brongn (Gramineae), namely 5-O-coumaroylquinic acid (i), 4-O-coumaroylquinic acid (ii), luteolin 6-C-β-d-galactopyranosiduronic acid (1→2)-β-d-glucopyranoside (iii), 7-O-β-d-glucopyranosyl-6-C-α-l-arabinopy ranoside (iv), isoorientin (v), swertiajaponin (vi), luteolin 6-C-β-d-galactopyranosiduronic acid (1→2)-α-l-arabinopyranoside (vii), Saponaretin (viii), swertisin (ix) and apigenin 6-C-β-d-galactopyranosiduronic acid (1→2)-α-l-arabinopyranoside (x). The analysis was performed on Cosmosil MS-IIⅡ C18 column (250 × 4.6 mm, 5 µm) with gradient elution of 0.1% aqueous acetic acid and acetonitrile. The detection wavelength was 330 nm. The developed method was able to determine the bioactive compounds with excellent resolution, precision and recovery. The validated method was successfully applied for the analysis of the 10 bioactive compounds in n samples from different cultivated regions. The results indicated that the developed method can be used as a suitable quality control method for L. gracile.
authors.affiliation: School of Chinese Medical Sciences, Southern Medical University, Guangzhou, PR China.
authors.forename: Qingfa
authors.initials: Q
authors.lastname: Tang
authors.affiliation: School of Chinese Medical Sciences, Southern Medical University, Guangzhou, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Shao
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
authors.forename: Huinan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Fan
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China.
authors.forename: Yaolan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, PR China Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, PR China chyewc@gmail.com.
authors.forename: Wencai
authors.initials: W
authors.lastname: Ye
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D010936:Plant Extracts / Q000737:chemistry*; D006109:Poaceae / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D003374:Coumarins; D005419:Flavonoids; D010936:Plant Extracts