title: Binding of trivalent chromium to serum transferrin is sufficiently rapid to be physiologically relevant.
abstract: Transferrin, the major iron transport protein in the blood, also transports trivalent chromium in vivo. Recent in vitro studies have, however, suggested that the binding of chromic ions to apotransferrin is too slow to be biologically relevant. Nevertheless, the in vitro studies have generally failed to adequately take physiological bicarbonate concentrations into account. In aqueous buffer (with ambient (bi)carbonate concentrations), the binding of chromium to transferrin is too slow to be physiologically relevant, taking days to reach equilibrium with the protein's associated conformational changes. However, in the presence of 25mM (bi)carbonate, the concentration in human blood, chromic ions bind rapidly and tightly to transferrin. Details of the kinetics of chromium binding to human serum transferrin and conalbumin (egg white transferrin) in the presence of bicarbonate and other major potential chromium ligands are described and are consistent with transferrin being the major chromic ion transporter from the blood to tissues.
authors.affiliation: Department of Chemistry, The University of Alabama, Tuscaloosa, AL 35487-0336, USA.
authors.forename: Ge
authors.initials: G
authors.lastname: Deng
authors.affiliation: Department of Chemistry, The University of Alabama, Tuscaloosa, AL 35487-0336, USA.
authors.forename: Kristi
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Chemistry, The University of Alabama, Tuscaloosa, AL 35487-0336, USA.
authors.forename: Alex A
authors.initials: AA
authors.lastname: Cruce
authors.affiliation: Department of Chemistry, The University of Alabama, Tuscaloosa, AL 35487-0336, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Bowman
authors.affiliation: Department of Chemistry, The University of Alabama, Tuscaloosa, AL 35487-0336, USA. Electronic address: jvincent@bama.ua.edu.
authors.forename: John B
authors.initials: JB
authors.lastname: Vincent
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000737:chemistry / Q000378:metabolism; D002645:Chickens; D002857:Chromium / Q000737:chemistry* / Q000378:metabolism; D003207:Conalbumin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D017136:Ion Transport / Q000502:physiology; D011485:Protein Binding; D014168:Transferrin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D014168:Transferrin; D002857:Chromium; D003207:Conalbumin
keywords: Chromium; Conalbumin; Kinetics; Transferrin