title: [Comparison of protective effects of eight ethyl acetate extracts from Eclipta prostrate on NHBE cells based on component structure theory].
abstract: To analyze and compare the protective effects of active components in different ethyl acetate extracts (EAEEPs) from Eclipta prostrate, in order to study the comparison of materials bases protecting normal human bronchial epithelial (NHBE) cells. The MTT assay was taken to compare the protective effect of different EAEEPs on cigarette smoke extracts (CSE) -induced NHBE cells. The ultra-performance liquid chromatography (UPLC) was applied to analyze the content of phenolic acid, coumaric grass ether and flavonoid in EAEEPs. According to the results, all of the eight EAEEPs (0-200 mg x L(-1)) showed certain protective effect on NHBE cells, with statistical difference. Specifically, the total mass of EAEEP VII (89.15 mg x L(-1)) and EAEEP VIII (57.44 mg x L(-1)), which showed the strongest activity, was not the highest, while EAEEP III (132.25 mg x L(-1)) displayed the highest total mass. In the combination with the "component structure" theory, the analysis showed a significant difference in the mass structure among phenolic acid, coumaric grass ether and flavonoid in EAEEP VIII and EAEEP VIII, which were 1.0: 1. 0: 0.5 and 1.0: 1.9: 0.8, respectively. The results suggested a specific optimal "component structure" relationship may exist in EAEEP, which could provide reference for the material base study and quality control.
authors.forename: Shu-Min
authors.initials: SM
authors.lastname: Ding
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Feng
authors.forename: Fen-Xia
authors.initials: FX
authors.lastname: Zhu
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Tan
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Jia
mesh_terms: D001980:Bronchi / Q000166:cytology* / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D036601:Eclipta / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D020011:Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*
chemical_list: D004365:Drugs, Chinese Herbal; D020011:Protective Agents; D014028:Tobacco Smoke Pollution