title: Glycocaulis alkaliphilus sp. nov., a dimorphic prosthecate bacterium isolated from crude oil.
abstract: A bacterial strain designated 6B-8(T) was isolated from crude oil from Daqing oilfield, China. Cells of strain 6B-8(T) were Gram-negative, aerobic, dimorphic and reproduced by means of binary fission. Strain 6B-8(T) could grow at 20-37 °C, pH 8-10 and 1-5 % (w/v) NaCl. Its genomic DNA G+C content was 62.0 mol%. The predominant cellular fatty acids were C18 : 1ω7c, C17 : 0, C18 : 0 and 11-methyl C18 : 1ω7c and the main hydroxy fatty acids were C12 : 0 3-OH and C12 : 1 3-OH when grown on marine agar 2216. The major quinone was Q-10 and the major polar lipids were three unidentified glycolipids. Phylogenetic analysis revealed that strain 6B-8(T) was a member of the family Hyphomonadaceae, sharing 99.6 and 99.4 % 16S rRNA gene sequence similarity with Glycocaulis abyssi LMG 27140(T) and Glycocaulis albus SLG210-30A1(T), respectively, and less than 94.4 % similarity with the type strains of other members of the family Hyphomonadaceae. However, the DNA-DNA relatedness between strain 6B-8(T) and related strains G. abyssi LMG 27140(T) and G. albus SLG210-30A1(T) was 36±5 and 42±5 %, respectively. In addition, several phenotypic and genotypic features allowed differentiation of strain 6B-8(T) from G. abyssi LMG 27140(T) and G. albus SLG210-30A1(T). Therefore, strain 6B-8(T) represents a novel species of genus Glycocaulis, for which the name Glycocaulis alkaliphilus sp. nov. is proposed. The type strain is 6B-8(T) ( = CGMCC 1.12428(T) = LMG 27410(T)).
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Geng
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Xin-Chi
authors.initials: XC
authors.lastname: Pan
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Mei
authors.affiliation: Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Wang
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Liu
authors.affiliation: College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Xing-Biao
authors.initials: XB
authors.lastname: Wang
authors.affiliation: College of Architecture and Environment, Sichuan University, Chengdu 610065, PR China.
authors.forename: Yue-Qin
authors.initials: YQ
authors.lastname: Tang
authors.affiliation: Institute of Engineering (Baotou), College of Engineering, Peking University, Baotou 014030, PR China.|College of Engineering, Peking University, Beijing 100871, PR China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wu
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010578:Petroleum / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010578:Petroleum; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2