title: Complementary metal oxide semiconductor compatible silicon nanowires-on-a-chip: fabrication and preclinical validation for the detection of a cancer prognostic protein marker in serum.
abstract: An integrated translational biosensing technology based on arrays of silicon nanowire field-effect transistors (SiNW FETs) is described and has been preclinically validated for the ultrasensitive detection of the cancer biomarker ALCAM in serum. High-quality SiNW arrays have been rationally designed toward their implementation as molecular biosensors. The FET sensing platform has been fabricated using a complementary metal oxide semiconductor (CMOS)-compatible process. Reliable and reproducible electrical performance has been demonstrated via electrical characterization using a custom-designed portable readout device. Using this platform, the cancer prognostic marker ALCAM could be detected in serum with a detection limit of 15.5 pg/mL. Importantly, the detection could be completed in less than 30 min and span a wide dynamic detection range (∼10(5)). The SiNW-on-a-chip biosensing technology paves the way to the translational clinical application of FET in the detection of cancer protein markers.
authors.affiliation: Ian Wark Research Institute, University of South Australia , Mawson Lakes Campus, Mawson Lakes, South Australia 5095, Australia.
authors.forename: Duy P
authors.initials: DP
authors.lastname: Tran
authors.forename: Bernhard
authors.initials: B
authors.lastname: Wolfrum
authors.forename: Regina
authors.initials: R
authors.lastname: Stockmann
authors.forename: Jing-Hong
authors.initials: JH
authors.lastname: Pai
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pourhassan-Moghaddam
authors.forename: Andreas
authors.initials: A
authors.lastname: Offenhäusser
authors.forename: Benjamin
authors.initials: B
authors.lastname: Thierry
mesh_terms: D015703:Antigens, CD / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D015816:Cell Adhesion Molecules, Neuronal / Q000097:blood; D005326:Fetal Proteins / Q000097:blood; D006801:Humans; D008670:Metals / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D009369:Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000628:therapy; D010087:Oxides / Q000737:chemistry*; D011379:Prognosis; D012666:Semiconductors; D012825:Silicon / Q000737:chemistry*; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D008670:Metals; D010087:Oxides; D012825:Silicon; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule