title: PEG-Poly(L-alanine) thermogel as a 3D scaffold of bone-marrow-derived mesenchymal stem cells.
abstract: Bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured in three-dimensional (3D) scaffolds formed by temperature-sensitive sol-to-gel transition of BMSC-suspended poly(ethylene glycol)-poly(L-alanine) (PEG-PA) aqueous solutions. A commercialized thermogelling 3D scaffold of Matrigel™ was used for the comparative study. The cells maintained their spherical shapes in the PEG-PA thermogel, whereas fibrous cell morphologies were observed in the Matrigel™. Type II collagen and myogenic differentiation factor 1 were dominantly expressed in the PEG-PA thermogel. On the other hand, a significant extent of type III β-tubulin was expressed in the Matrigel™ in addition to type II collagen and myogenic differentiation factor 1. After confirming the dominant chondrogenic differentiation of the BMSCs in the PEG-PA thermogel in in vitro study, in vivo study was performed for injectable tissue engineering application of the BMSCs/PEG-PA system. The cell-growing implant was formed in situ by subcutaneous injection of the BMSC-suspended PEG-PA aqueous solution to mice. In vivo study also proved the excellent expressions of chondrogenic biomarkers including collagen type II and sulfated glycosaminoglycan in the mouse model. This paper suggests that the PEG-PA thermogel is a very promising as a 3D culture matrix as well as an injectable tissue-engineering system for preferential chondrogenic differentiation of the BMSCs.
authors.affiliation: Department of Chemistry and Nano Science, Ewha Global Top 5 Research Program, Ewha Womans University, 52 Ewhayeodae-gil, Seodaemun-gu, Seoul, 120-750, Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Park
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Jin Hye
authors.initials: JH
authors.lastname: Park
authors.forename: Usha Pramod
authors.initials: UP
authors.lastname: Shinde
authors.forename: Du Young
authors.initials: du Y
authors.lastname: Ko
authors.forename: Byeongmoon
authors.initials: B
authors.lastname: Jeong
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D020219:Chondrogenesis; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D066298:In Vitro Techniques; D007797:Laminin / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011509:Proteoglycans / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D004338:Drug Combinations; D007797:Laminin; D010455:Peptides; D011509:Proteoglycans; C059485:matrigel; C019529:polyalanine; D011092:Polyethylene Glycols; D003094:Collagen
keywords: 3D culture; BMSC; hydrogel; sol-gel transition