title: Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome.
abstract: Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of prefractionation steps prior to MS analysis. More than 100 plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process. All MS data have been deposited in the ProteomeXchange with identifier PXD001117 (http://proteomecentral.proteomexchange.org/dataset/PXD001117).
authors.affiliation: iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Gomes-Alves
authors.forename: Margarida
authors.initials: M
authors.lastname: Serra
authors.forename: Catarina
authors.initials: C
authors.lastname: Brito
authors.forename: Luis
authors.initials: L
authors.lastname: R-Borlado
authors.forename: Juan A
authors.initials: JA
authors.lastname: López
authors.forename: Jesús
authors.initials: J
authors.lastname: Vázquez
authors.forename: Manuel J T
authors.initials: MJ
authors.lastname: Carrondo
authors.forename: António
authors.initials: A
authors.lastname: Bernad
authors.forename: Paula M
authors.initials: PM
authors.lastname: Alves
mesh_terms: D053687:Adult Stem Cells / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D006801:Humans; D009203:Myocardial Infarction / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000473:pathology; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D040901:Proteomics; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012038:Regeneration; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome; D011956:Receptors, Cell Surface
keywords: Biomedicine; Human cardiac stem cells; Nano-LC-MS/MS; Prefractionation; Receptome