title: funRNA: a fungi-centered genomics platform for genes encoding key components of RNAi.
abstract: BACKGROUND
  RNA interference (RNAi) is involved in genome defense as well as diverse cellular, developmental, and physiological processes. Key components of RNAi are Argonaute, Dicer, and RNA-dependent RNA polymerase (RdRP), which have been functionally characterized mainly in model organisms. The key components are believed to exist throughout eukaryotes; however, there is no systematic platform for archiving and dissecting these important gene families. In addition, few fungi have been studied to date, limiting our understanding of RNAi in fungi. Here we present funRNA http://funrna.riceblast.snu.ac.kr/, a fungal kingdom-wide comparative genomics platform for putative genes encoding Argonaute, Dicer, and RdRP.
  
  
  METHODS
  To identify and archive genes encoding the abovementioned key components, protein domain profiles were determined from reference sequences obtained from UniProtKB/SwissProt. The domain profiles were searched using fungal, metazoan, and plant genomes, as well as bacterial and archaeal genomes. 1,163, 442, and 678 genes encoding Argonaute, Dicer, and RdRP, respectively, were predicted. Based on the identification results, active site variation of Argonaute, diversification of Dicer, and sequence analysis of RdRP were discussed in a fungus-oriented manner. funRNA provides results from diverse bioinformatics programs and job submission forms for BLAST, BLASTMatrix, and ClustalW. Furthermore, sequence collections created in funRNA are synced with several gene family analysis portals and databases, offering further analysis opportunities.
  
  
  CONCLUSIONS
  funRNA provides identification results from a broad taxonomic range and diverse analysis functions, and could be used in diverse comparative and evolutionary studies. It could serve as a versatile genomics workbench for key components of RNAi.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Choi
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Kim
authors.forename: Jongbum
authors.initials: J
authors.lastname: Jeon
authors.forename: Jiayao
authors.initials: J
authors.lastname: Wu
authors.forename: Hyeunjeong
authors.initials: H
authors.lastname: Song
authors.forename: Fred O
authors.initials: FO
authors.lastname: Asiegbu
authors.forename: Yong-Hwan
authors.initials: YH
authors.lastname: Lee
mesh_terms: D060565:Argonaute Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D030541:Databases, Genetic; D019143:Evolution, Molecular; D020440:Gene Duplication; D005800:Genes, Fungal / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D034622:RNA Interference; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D060565:Argonaute Proteins; D012324:RNA-Dependent RNA Polymerase; D043244:Ribonuclease III