title: Amyloid form of ovalbumin evokes native antigen-specific immune response in the host: prospective immuno-prophylactic potential.
abstract: Amyloids are highly organized protein aggregates that arise from inappropriately folded versions of proteins or polypeptides under both physiological as well as simulated ambiences. Once thought to be irreversible assemblies, amyloids have begun to expose their more dynamic and reversible attributes depending upon the intrinsic properties of the precursor protein/peptide and experimental conditions such as temperature, pressure, structural modifications in proteins, or presence of chemicals in the reaction mixture. It has been repeatedly proposed that amyloids undergo transformation to the bioactive peptide/protein forms under specific conditions. In the present study, amyloids assembled from the model protein ovalbumin (OVA) were found to release the precursor protein in a slow and steady manner over an extended time period. Interestingly, the released OVA from amyloid depot was found to exhibit biophysical characteristics of native protein and reacted with native-OVA specific monoclonal as well as polyclonal antibodies. Moreover, antibodies generated upon immunization of OVA amyloidal aggregates or fibrils were found to recognize the native form of OVA. The study suggests that amyloids may act as depots for the native form of the protein and therefore can be exploited as vaccine candidates, where slow antigen release over extended time periods is a pre-requisite for the development of desired immune response.
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002.
authors.forename: Saba
authors.initials: S
authors.lastname: Tufail
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002, owais_lakhnawi@yahoo.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Owais
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002.
authors.forename: Shadab
authors.initials: S
authors.lastname: Kazmi
authors.affiliation: the National Institute of Immunology, New Delhi.
authors.forename: Renu
authors.initials: R
authors.lastname: Balyan
authors.affiliation: the National Institute of Immunology, New Delhi.
authors.forename: Jasneet Kaur
authors.initials: JK
authors.lastname: Khalsa
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002.
authors.forename: Syed Mohd
authors.initials: SM
authors.lastname: Faisal
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002.
authors.forename: Mohd Asif
authors.initials: MA
authors.lastname: Sherwani
authors.affiliation: the Department of Biochemistry, JNMC, Aligarh Muslim University, Aligarh, U.P.-202002, and.
authors.forename: Manzoor Ahmad
authors.initials: MA
authors.lastname: Gatoo
authors.affiliation: From the Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, U.P.-202002.
authors.forename: Mohd Saad
authors.initials: MS
authors.lastname: Umar
authors.affiliation: the Women's College, Aligarh Muslim University, Aligarh, U.P.-202002, India swalehazubair@yahoo.com.
authors.forename: Swaleha
authors.initials: S
authors.lastname: Zubair
mesh_terms: D000682:Amyloid / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000276:immunology*; D002942:Circular Dichroism; D016207:Cytokines / Q000378:metabolism; D005260:Female; D007114:Immunization; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D009569:Nitric Oxide / Q000378:metabolism; D010047:Ovalbumin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D011487:Protein Conformation; D055503:Protein Multimerization; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000682:Amyloid; D000911:Antibodies, Monoclonal; D016207:Cytokines; D010455:Peptides; D009569:Nitric Oxide; D010047:Ovalbumin
keywords: Albumin; Amyloid; Antibody; Antigen; Antigen Presentation