title: Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination.
abstract: Pollination is an important early step in sexual plant reproduction. In Arabidopsis thaliana, sequential pollination events, from pollen adhesion onto the stigma surface to pollen tube germination and elongation, occur on the stigmatic papilla cells. Following successful completion of these events, the pollen tube penetrates the stigma and finally fertilizes a female gametophyte. The pollination events are thought to be initiated and regulated by interactions between papilla cells and pollen. Here, we report the characterization of gene expression profiles of unpollinated (UP), compatible pollinated (CP) and incompatible pollinated (IP) papilla cells in A. thaliana. Based on cell type-specific transcriptome analysis from a combination of laser microdissection and RNA sequencing, 15,475, 17,360 and 16,918 genes were identified as expressed in UP, CP and IP papilla cells, respectively, and, of these, 14,392 genes were present in all three data sets. Differentially expressed gene (DEG) analyses identified 147 and 71 genes up-regulated in CP and IP papilla cells, respectively, and 115 and 46 genes down-regulated. Gene Ontology and metabolic pathway analyses revealed that papilla cells play an active role as the female reproductive component in pollination, particularly in information exchange, signal transduction, internal physiological changes and external morphological modification. This study provides fundamental information on the molecular mechanisms involved in pollination in papilla cells, furthering our understanding of the reproductive role of papilla cells.
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Matsushima
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
authors.forename: Moe
authors.initials: M
authors.lastname: Nabemoto
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Osaka
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Sakazono
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Masuko-Suzuki
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601 Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nakazono
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192 Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Iwano
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, 630-0192 Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Takayama
authors.affiliation: Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
authors.forename: Kentaro K
authors.initials: KK
authors.lastname: Shimizu
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan.
authors.forename: Katsuzumi
authors.initials: K
authors.lastname: Okumura
authors.affiliation: Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582 Japan.
authors.forename: Go
authors.initials: G
authors.lastname: Suzuki
authors.affiliation: Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Graduate School of Bioresources, Mie University, Tsu, 514-8507 Japan suwabe@bio.mie-u.ac.jp.
authors.forename: Keita
authors.initials: K
authors.lastname: Suwabe
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics* / Q000502:physiology; D053898:Biosynthetic Pathways / Q000235:genetics; D035264:Flowers / Q000166:cytology* / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways / Q000235:genetics; D054817:Pollination / Q000235:genetics*; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic; D059467:Transcriptome
keywords: Arabidopsis thaliana; Laser microdissection; Papilla cell; Pollination; RNA sequencing; Transcriptome