title: Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation.
abstract: The biological hydrogen production from amino acids obtained by protein degradation was comprehensively investigated to increase heating value conversion efficiency. The five amino acids (i.e., alanine, serine, aspartic acid, arginine, and leucine) produced limited hydrogen (0.2-16.2 mL/g) but abundant soluble metabolic products (40.1-84.0 mM) during dark-fermentation. The carbon conversion efficiencies of alanine (85.3%) and serine (94.1%) during dark-fermentation were significantly higher than those of other amino acids. Residual dark-fermentation solutions treated with zeolite for NH4(+) removal were inoculated with photosynthetic bacteria to further produce hydrogen during photo-fermentation. The hydrogen yields of alanine and serine through combined dark- and photo-fermentation were 418.6 and 270.2 mL/g, respectively. The heating value conversion efficiency of alanine to hydrogen was 25.1%, which was higher than that of serine (21.2%).
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Lingkan
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Ao
authors.initials: A
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Richen
authors.initials: R
authors.lastname: Lin
authors.affiliation: Department of Civil and Environmental Engineering, Tohoku University, Sendai 9808579, Japan.
authors.forename: Yuyou
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D003624:Darkness; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D059748:Proteolysis; D012995:Solubility; D012996:Solutions; D014865:Waste Disposal, Fluid; D014866:Waste Products / Q000032:analysis*
chemical_list: D000596:Amino Acids; D012996:Solutions; D014866:Waste Products; D002244:Carbon; D006859:Hydrogen
keywords: Amino acid; Dark-fermentation; Hydrogen production; Photo-fermentation