title: In vitro and in vivo antimicrobial efficacy of natural plant-derived compounds against Vibrio cholerae of O1 El Tor Inaba serotype.
abstract: In this study, we investigated antibacterial activities of 20 plant-derived natural compounds against Gram-negative enteric pathogens. We found that both flavonoids and non-flavonoids, including honokiol and magnolol, possess specific antibacterial activities against V. cholerae, but not against other species of Gram-negative bacterium which we tested. Using various antibacterial assays, we determined that there was a dose-dependent bactericidal and biofilm inhibitory activity of honokiol and magnolol against Vibrio cholerae. In addition to antibacterial activities, these molecules also induced an attenuating effect on reactive oxygen species (ROS) production and pro-inflammatory responses generated by macrophages in response to lipopolysaccharides (LPS). Additionally, Caenorhabditis elegans lethality assay revealed that honokiol and magnolol have an ability to extend a lifespan of V. cholerae-infected worms, contributing to prolonged survival of worms after lethal infection. Altogether, our data show for the first time that honokiol and magnolol may be considered as attractive protective or preventive food adjuncts for cholera.
authors.affiliation: a Department of Biomedical Sciences , College of Medicine, Korea University , Seoul , Republic of Korea.
authors.forename: Hyung-Ip
authors.initials: HI
authors.lastname: Kim
authors.forename: Ji-Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Choi
authors.forename: Jason B
authors.initials: JB
authors.lastname: Harris
authors.forename: Seong-Yeop
authors.initials: SY
authors.lastname: Jeong
authors.forename: Seok-Jun
authors.initials: SJ
authors.lastname: Son
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kim
authors.forename: Ok Sarah
authors.initials: OS
authors.lastname: Shin
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects; D001688:Biological Products / Q000494:pharmacology* / Q000627:therapeutic use; D017173:Caenorhabditis elegans; D002771:Cholera / Q000188:drug therapy; D005502:Food; D008070:Lipopolysaccharides / Q000009:adverse effects; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D000067996:RAW 264.7 Cells; D014734:Vibrio cholerae / Q000187:drug effects* / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D001688:Biological Products; D008070:Lipopolysaccharides; D010936:Plant Extracts
keywords: C. elegans; V. cholerae; antibacterial effect; honokiol; magnolol