title: Effect of donor chimerism to reduce the level of glycosaminoglycans following bone marrow transplantation in a murine model of mucopolysaccharidosis type II.
abstract: Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder caused by deficient activity of the iduronate-2-sulfatase. This leads to accumulation of glycosaminoglycans (GAGs) in the lysosomes of various cells. Although it has been proposed that bone marrow transplantation (BMT) may have a beneficial effect for patients with MPS II, the requirement for donor-cell chimerism to reduce GAG levels is unknown. To address this issue, we transplanted various ratios of normal and MPS II bone marrow cells in a mouse model of MPS II and analyzed GAG accumulation in various tissues. Chimerism of whole leukocytes and each lineage of BMT recipients' peripheral blood was similar to infusion ratios. GAGs were significantly reduced in the liver, spleen, and heart of recipients. The level of GAG reduction in these tissues depends on the percentage of normal-cell chimerism. In contrast to these tissues, a reduction in GAGs was not observed in the kidney and brain, even if 100 % donor chimerism was achieved. These observations suggest that a high degree of chimerism is necessary to achieve the maximum effect of BMT, and donor lymphocyte infusion or enzyme replacement therapy might be considered options in cases of low-level chimerism in MPS II patients.
authors.affiliation: Department of Pediatrics, The Jikei University School of Medicine, 3-25-8 Nishishimbashi, Minato-ku, Tokyo, 105-8461, Japan, kenta451@jikei.ac.jp.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yokoi
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Akiyama
authors.forename: Eiko
authors.initials: E
authors.lastname: Kaneshiro
authors.forename: Takashi
authors.initials: T
authors.lastname: Higuchi
authors.forename: Yohta
authors.initials: Y
authors.lastname: Shimada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Masaharu
authors.initials: M
authors.lastname: Akiyama
authors.forename: Makoto
authors.initials: M
authors.lastname: Otsu
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Toya
authors.initials: T
authors.lastname: Ohashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ida
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D006025:Glycosaminoglycans / Q000378:metabolism*; D007066:Iduronate Sulfatase / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008297:Male; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D016532:Mucopolysaccharidosis II / Q000201:enzymology / Q000235:genetics / Q000601:surgery*; D009206:Myocardium / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013997:Time Factors; D018183:Transplantation Chimera
chemical_list: D006025:Glycosaminoglycans; D007066:Iduronate Sulfatase