title: Differential spheroid formation by oral cancer cells.
abstract: Squamous cell carcinomas (SCC) make up 96% of all oral cancers. Most laboratory SCC studies grow cells as a monolayer, which does not accurately represent the disease in vivo. We used a more relevant multicellular spheroid (MCS) model to study this disease. The SCC9β6KDFyn cell line, which expresses full-length β6 and a kinase dead Fyn formed the largest MCS. Cell adhesive properties are dynamic and N-cadherin was increased in the largest MCS. c-Raf mediates the survival of tumor cells and was consistently expressed both in monolayers and in the MCS by SCC9β6D1 cells which lack the β6 cytoplasmic tail and, do not activate Fyn. SCC9β6KDFyn cells also express high levels of c-Raf when grown as spheroids in which Fyn suppression stimulates MCS formation. Tumor microenvironment and growth patterns modulate cell behavior and suppression of Fyn kinase may promote MCS growth.
authors.affiliation: Department of Orofacial Sciences, University of California at San Francisco, San Francisco, CA, U.S.A.
authors.forename: Carlin
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Orofacial Sciences, University of California at San Francisco, San Francisco, CA, U.S.A.
authors.forename: Casey
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Medicine, Lung Biology Center, University of California, San Francisco, CA, U.S.A.
authors.forename: Amha
authors.initials: A
authors.lastname: Atakilit
authors.affiliation: Department of Pediatric Dentistry, University of Texas, Health Sciences Center, San Antonio, TX, U.S.A.
authors.forename: Amanda
authors.initials: A
authors.lastname: Siu
authors.affiliation: Department of Orofacial Sciences, University of California at San Francisco, San Francisco, CA, U.S.A. Daniel.ramos@ucsf.edu.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Ramos
mesh_terms: D015820:Cadherins / Q000096:biosynthesis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D006801:Humans; D039641:Integrin beta Chains / Q000096:biosynthesis*; D009062:Mouth Neoplasms / Q000473:pathology*; D051076:Proto-Oncogene Proteins c-fyn / Q000096:biosynthesis*; D019908:Proto-Oncogene Proteins c-raf / Q000096:biosynthesis; D015398:Signal Transduction; D018874:Spheroids, Cellular / Q000473:pathology*; D014407:Tumor Cells, Cultured; D059016:Tumor Microenvironment
chemical_list: D015820:Cadherins; D039641:Integrin beta Chains; C072170:integrin beta6; C494957:FYN protein, human; D051076:Proto-Oncogene Proteins c-fyn; D019908:Proto-Oncogene Proteins c-raf
keywords: E-cadherin; N-cadherin; Spheroid; c-Raf