title: [Proposal of a five MIRU-VNTR panel to screen clinical isolates of Mycobacterium tuberculosis in Mexico].
abstract: BACKGROUND
  Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates.
  
  
  METHODS
  In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected.
  
  
  RESULTS
  The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin.
  
  
  CONCLUSIONS
  The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico.
authors.affiliation: Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México. Electronic address: ebolado@guayacan.uson.mx.
authors.forename: Enrique
authors.initials: E
authors.lastname: Bolado-Martínez
authors.affiliation: Departamento de Medicina, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Maria Del Carmen
authors.initials: Mdel C
authors.lastname: Candia-Plata
authors.affiliation: Instituto de Salud Pública, Universidad Veracruzana, Xalapa, Veracruz, México.
authors.forename: Roberto
authors.initials: R
authors.lastname: Zenteno-Cuevas
authors.affiliation: Laboratorio Estatal de Salud Pública del Estado de México, Toluca, México.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Mendoza Damián
authors.affiliation: Laboratorio Estatal de Salud Pública, Hermosillo, Sonora, México.
authors.forename: Magali
authors.initials: M
authors.lastname: Avilés-Acosta
authors.affiliation: Departamento de Medicina, Universidad de Sonora, Hermosillo, Sonora, México.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Álvarez-Hernández
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D060005:Genotyping Techniques; D062306:Geography, Medical; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008403:Mass Screening / Q000379:methods*; D008800:Mexico / Q000453:epidemiology; D018598:Minisatellite Repeats; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial
keywords: MIRU-VNTR; Mexico; Mycobacterium tuberculosis; México