title: Q192R polymorphism of paraoxonase 1 gene associated with insulin resistance in Mexican children.
abstract: OBJECTIVE
  The SNP rs662 in the paraoxonase 1 gene (PON1 Q192R) has been associated with obesity, dyslipidemia and cardiovascular risk. In this study, DNA samples of 117 children aged 6 to 12 years from San Luis Potosí, México were genotyped for Q192R polymorphism of the PON1 gene.
  
  
  METHODS
  Genotypic frequencies were determined by allelic discrimination assay by real-time PCR using TaqMan fluorogenic probes. Anthropometry, lipid profile, glucose and insulin were analyzed by genotype.
  
  
  RESULTS
  The distribution of allele frequency in the population was Q = 65 and R = 35 following the Hardy Weinberg equilibrium (χ(2) = 3.15, p = 0.076). The Homeostasis Model Assessment for Insulin Resistance (HOMA-IR) index showed statistically significant differences among QQ/QR/RR genotypes (p = 0.032). The odds ratio for the carriers of the RR genotype was associated with HOMA-IR corresponding to the 95(th) percentile or higher for Mexican children based on sex and age (OR = 4.68; 95% confidence intervals, 1.23-17.8; p = 0.016). When the absolute mean of HOMAR-IR was set as the cutoff, an increased odds was observed (OR = 6.52; 95% confidence intervals, 1.68-25.3; p = 0.008).
  
  
  CONCLUSIONS
  According to our results, PON1 Q192R polymorphism is a risk marker for insulin resistance, a pathological factor involved in the development of metabolic syndrome.
authors.affiliation: Laboratorio de Investigación Molecular en Nutrición (LIMON), Universidad del Centro de México UCEM, San Luis Potosí, México. Electronic address: giorgio_alegretto@hotmail.com.
authors.forename: Jorge Alejandro
authors.initials: JA
authors.lastname: Alegría-Torres
authors.affiliation: Laboratorio de Investigación Molecular en Nutrición (LIMON), Universidad del Centro de México UCEM, San Luis Potosí, México.
authors.forename: María L
authors.initials: ML
authors.lastname: García-Domínguez
authors.affiliation: Unidad de Investigación Médica en Bioquímica, Hospital de Especialidades, Centro Médico Nacional Siglo XXI, IMSS, México D.F., México.
authors.forename: Miguel
authors.initials: M
authors.lastname: Cruz
authors.affiliation: Centro de Investigación Aplicada en Ambiente y Salud, CIACYT-Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.
authors.forename: Celia
authors.initials: C
authors.lastname: Aradillas-García
mesh_terms: D043303:Aryldialkylphosphatase / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000235:genetics; D002648:Child; D050171:Dyslipidemias / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics*; D008297:Male; D024821:Metabolic Syndrome / Q000235:genetics; D008800:Mexico; D009765:Obesity / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012306:Risk; D012307:Risk Factors
chemical_list: D005819:Genetic Markers; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human
keywords: Insulin resistance; Metabolic syndrome; Obesity; PON 1 gene