title: α-Conotoxin M1 (CTx) blocks αδ binding sites of adult nicotinic receptors while ACh binding at αε sites elicits only small and short quantal synaptic currents.
abstract: In 'embryonic' nicotinic receptors, low CTx concentrations are known to block only the αδ binding site, whereas binding of ACh at the αγ-site elicits short single channel openings and short bursts. In adult muscles the αγ- is replaced by the αε-site. Quantal EPSCs (qEPSCs) were elicited in adult muscles by depolarization pulses and recorded through a perfused macropatch electrode. One to 200 nmol L(-1) CTx reduced amplitudes and decay time constants of qEPSCs, but increased their rise times. CTx block at the αδ binding sites was incomplete: The qEPSCs still contained long bursts from not yet blocked receptors, whereas their average decay time constants were reduced by a short burst component generated by ACh binding to the αε-site. Two nanomolar CTx applied for 3 h reduced the amplitudes of qEPSCs to less than half with a constant slope. The equilibrium concentration of the block is below 1 nmol L(-1) and lower than that of embryonic receptors. CTx-block increased in proportion to CTx concentrations (average rate 2 ×: 10(4) s(-1)·mol(-1) L). Thus, the reactions of 'embryonic' and of adult nicotinic receptors to block by CTx are qualitatively the same. - The study of the effects of higher CTx concentrations or of longer periods of application of CTx was limited by presynaptic effects of CTx. Even low CTx concentrations severely reduced the release of quanta by activating presynaptic M2 receptors at a maximal rate of 6 ×: 10(5) s(-1)·mol(-1) L. When this dominant inhibition was prevented by blocking the M2 receptors with methoctramine, activation of M1 receptors was unmasked and facilitated release.
authors.affiliation: Institut für Neurowissenschaften, Technische Universität München, Biedersteinerstr. 29, München, D-80802, Germany.
authors.forename: Josef
authors.initials: J
authors.lastname: Dudel
keywords: CTx block transmission; binding site specificity; mouse nicotinic receptor; presynaptic muscarinic receptors