title: Effects of rizatriptan on the expression of calcitonin gene-related peptide and cholecystokinin in the periaqueductal gray of a rat migraine model.
abstract: Triptans are serotonin 5-hydroxytryptamine receptor 1B/D agonists that are highly effective in the treatment of migraine. We previously found that rizatriptan can reduce the expression of proenkephalin and P substance in the rat midbrain, suggesting that rizatriptan may exert its analgesic effects by influencing the endogenous pain modulatory system. Calcitonin gene-related peptide (CGRP) and cholecystokinin (CCK) are mainly responsible for antagonizing the analgesic effects of opioid peptides in the endogenous pain modulatory system. In this study, we investigated the effects of rizatriptan on the expression of CGRP and CCK in the periaqueductal gray (PAG), a key structure of the endogenous pain modulatory system, in a rat migraine model induced by nitroglycerin. We found that the mRNA and protein levels of CGRP and CCK in the PAG of migraine rats were significantly increased compared to those in control rats, and these levels were significantly reduced upon treatment with rizatriptan in migraine rats (P<0.05). Our results suggest that the expression of CGRP and CCK in the endogenous pain modulatory system may be increased during migraine attacks, which further antagonizes the analgesic effects of endogenous opioid peptides and induces sustained migraine. Rizatriptan, however, significantly reduces the levels of CGRP and CCK to enhance the inhibition of pain signals via the endogenous pain modulatory system, resulting in effective treatment of migraine.
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Changchun 130041, Jilin, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Yao
authors.affiliation: Department of Neurology, Chifeng Municipal Hospital, Chifeng 024000, Inner Mongolia Autonomous Region, China.
authors.forename: Ximei
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Nuclear Medicine, The Second Hospital of Jilin University, Changchun 130041, Jilin, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hao
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Changchun 130041, Jilin, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Neurology, The Second Hospital of Jilin University, Changchun 130041, Jilin, China. Electronic address: ytm_396@163.com.
authors.forename: Tingmin
authors.initials: T
authors.lastname: Yu
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000235:genetics / Q000378:metabolism*; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism*; D005260:Female; D008297:Male; D008881:Migraine Disorders / Q000378:metabolism*; D010487:Periaqueductal Gray / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D044302:Receptor, Serotonin, 5-HT1B / Q000378:metabolism; D044346:Receptor, Serotonin, 5-HT1D / Q000378:metabolism; D058825:Serotonin 5-HT1 Receptor Agonists / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*; D014363:Tryptamines / Q000494:pharmacology*
chemical_list: D012333:RNA, Messenger; D044302:Receptor, Serotonin, 5-HT1B; D044346:Receptor, Serotonin, 5-HT1D; D058825:Serotonin 5-HT1 Receptor Agonists; D014230:Triazoles; D014363:Tryptamines; C093622:rizatriptan; D002766:Cholecystokinin; D015740:Calcitonin Gene-Related Peptide
keywords: Calcitonin gene-related peptide; Cholecystokinin; Endogenous pain modulatory system; Migraine; Rizatriptan