title: Serum microRNAs in clear cell carcinoma of the ovary.
abstract: OBJECTIVE
  To identify candidate microRNAs (miRNAs) in the serum of patients with clear cell carcinomas in monitoring disease progression.
  
  
  METHODS
  The sera of patients with diagnosed ovarian clear cell carcinoma were collected from 2009 to 2012. Real-time quantitative polymerase chain reaction (PCR) analysis for 270 miRNAs was performed. To offset the potential extraction bias, an equal amount of Caenorhabditis elegans cel-miR-238 was added to each serum specimen before miRNA isolation. miRNA expression was analyzed using the ΔCt method, with cel-miR-238 as controls.
  
  
  RESULTS
  Twenty-one patients with clear cell carcinoma were included. In the discovery phase on four pairs of pre- and postoperative sera, 18 differentially expressed miRNAs were selected from 270 miRNAs. In the validation phase on an independent set of 11 pairs of pre- and postoperative sera, 4 miRNAs (hsa-miR-130a, hsa-miR-138, hsa-miR-187, and hsa-miR-202) were confirmed to be higher in the preoperative sera. In the application phase, hsa-miR-130a remained consistent with the different time points in seven of the 10 patients during clinical follow-up periods. More importantly, in three patients, hsa-miR-130a levels were elevated in early disease recurrences before CA125 was found to be elevated.
  
  
  CONCLUSIONS
  Hsa-miR-130a may be a useful serum biomarker for detecting recurrence of ovarian clear cell cancer, and warrants further studies.
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Angel
authors.initials: A
authors.lastname: Chao
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Chyong-Huey
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan; Department of Biomedical Science, School of Medicine, Chang Gung University, Taoyuan, Taiwan.
authors.forename: Hua-Chien
authors.initials: HC
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Chiao-Yun
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Chia-Lung
authors.initials: CL
authors.lastname: Tsai
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Yun-Hsin
authors.initials: YH
authors.lastname: Tang
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Huei-Jean
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Chen-Tao
authors.initials: CT
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Min-Yu
authors.initials: MY
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Kuang-Gen
authors.initials: KG
authors.lastname: Huang
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Hung-Hsueh
authors.initials: HH
authors.lastname: Chou
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan.
authors.forename: Ting-Chang
authors.initials: TC
authors.lastname: Chang
authors.affiliation: Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan; Department of Biomedical Science, School of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address: sjchen@mail.cgu.edu.tw.
authors.forename: Shu-Jen
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Gynecologic Cancer Research Centre, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taoyuan, Taiwan; School of Traditional Chinese Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan. Electronic address: knoxtn@cgmh.org.tw.
authors.forename: Tzu-Hao
authors.initials: TH
authors.lastname: Wang
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology; D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C554019:MIRN130 microRNA, human; D035683:MicroRNAs
keywords: clear cell carcinoma; hsa-miR-130a; microRNA; ovarian cancer