title: [Novel cationic liposome loading siRNA inhibits the expression of hepatitis B virus HBx gene].
abstract: In order to solve the problem of selection and in vivo delivery problem in siRNA treatment, hepatitis B virus (HBV) HBx gene which could be targeted by siRNA was studied. The siRNA expression plasmid which specific inhibits HBx expression was obtained by in vitro selection via a dual-luciferase plasmid including HBx-Fluc fusion protein expression domain. The selected siRNA expression plasmid was then encapsulated in PEG-modified cationic liposome, which was devoted into pharmacodynamic studies at both cellular and animal level. The results illustrated that the cationic liposome which encapsulated siRNA expression plasmid could effectively inhibit HBx gene expression both in vitro and in vivo.
authors.forename: Ya-Juan
authors.initials: YJ
authors.lastname: Wang
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Gao
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Ni
authors.forename: Chan
authors.initials: C
authors.lastname: Li
authors.forename: Tian-Jiao
authors.initials: TJ
authors.lastname: Wen
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Li
mesh_terms: D002412:Cations; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D006515:Hepatitis B virus / Q000235:genetics; D008081:Liposomes / Q000737:chemistry*; D010957:Plasmids; D034741:RNA, Small Interfering / Q000737:chemistry*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D002412:Cations; D008081:Liposomes; D034741:RNA, Small Interfering; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein