title: A comparative analysis on the binding characteristics of various mammalian albumins towards a multitherapeutic agent, pinostrobin.
abstract: The interaction of pinostrobin (PS), a multitherapeutic agent with serum albumins of various mammalian species namely, goat, bovine, human, porcine, rabbit, sheep and dog was investigated using fluorescence quench titration and competitive drug displacement experiments. Analysis of the intrinsic fluorescence quenching data revealed values of the association constant, K(a) in the range of 1.49 - 6.12 × 10(4) M(-1), with 1:1 binding stoichiometry. Based on the PS-albumin binding characteristics, these albumins were grouped into two classes. Ligand displacement studies using warfarin as the site I marker ligand correlated well with the binding data. Albumins from goat and bovine were found to be closely similar to human albumin on the basis of PS binding characteristics.
authors.affiliation: Biomolecular Research Group, Biochemistry Programme, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Shevin R
authors.initials: SR
authors.lastname: Feroz
authors.forename: Rumana A
authors.initials: RA
authors.lastname: Sumi
authors.forename: Sri N A
authors.initials: SN
authors.lastname: Malek
authors.forename: Saad
authors.initials: S
authors.lastname: Tayyab
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004285:Dogs; D005018:Ethyl Chloride; D044950:Flavanones / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D023421:Models, Animal; D015394:Molecular Structure; D011485:Protein Binding; D011817:Rabbits; D012709:Serum Albumin / Q000378:metabolism*; D012756:Sheep; D013045:Species Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D013552:Swine
chemical_list: D044950:Flavanones; D012709:Serum Albumin; D005018:Ethyl Chloride; C411294:pinostrobin