title: Pectin/lysozyme bilayers layer-by-layer deposited cellulose nanofibrous mats for antibacterial application.
abstract: Positively charged lysozyme (LZ) and negatively charged pectin, were alternately deposited on the surface of the cellulose nanofibrous mats by layer-by-layer (LBL) self-assembly technique. Scanning electron microscopy images showed that the nanofibers were orderly and compactly arrayed after LBL. Besides, as the number of LZ/pectin bilayers increased, the average diameter of nanofibers increased. LZ has assembled on the cellulose mats successfully, which was confirmed by X-ray photoelectron spectroscopy analysis. Thermal gravimetric analysis results showed that the thermal properties of LZ/pectin films coated mats was better than that of the unmodified cellulose mats. Importantly, the results of the bacterial inhibition test for LBL structured mats and cellulose mats indicated that the nanofibrous mats coated by 10.5 LZ/pectin bilayers (with LZ on the outmost layer) possessed the strongest inhibitory effect against both Escherichia coli and Staphylococcus aureus.
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; College of Food Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Wuhan 430070, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Panghu
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yingfei
authors.initials: Y
authors.lastname: Zhan
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Du
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China. Electronic address: lixiuhong@sinap.ac.cn.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Environmental Science, Hubei Key Lab of Biomass Resource Chemistry and Environmental Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China; College of Food Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Road, Wuhan 430070, China. Electronic address: hbdeng@whu.edu.cn.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Deng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002482:Cellulose / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D009113:Muramidase / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010368:Pectins / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D010368:Pectins; D002482:Cellulose; D009113:Muramidase
keywords: Bacterial inhibition ability; Layer-by-layer; Lysozyme; Nanofibrous mats; Pectin