title: Serum macrophage migration inhibitory factor levels in Hashimoto's thyroiditis; a case control study.
abstract: OBJECTIVE
  The cell-mediated immune process by CD4+ and CD8+ lymphocyte subsets of T-cells has a major role in the pathogenesis of Hashimoto's thyroiditis (HT). However, the exact mechanisms of initiation and progression of thyroid autoimmunity have not been completely clarified yet. Macrophage migration inhibitory factor (MIF) is commonly recognized as playing vital roles in various autoimmune diseases. Ee aimed to investigate serum MIF levels in subjects with HT and correlate them with the level of thyroid hormones and autoantibodies.
  
  
  METHODS
  This study included 93 patients with untreated Hashimoto's thyroiditis and 53 healthy controls. We measured serum levels of TSH, free T4 (FT4), free T3 (FT3), anti-thyroglobulin autoantibody (TGAb) and anti-thyroid peroxidase autoantibody (TPOAb) in all patients and thyroid ultrasonography was performed. The concentration of MIF was measured using enzyme-linked immunosorbent assay (ELISA) method.
  
  
  RESULTS
  We enrolled 93 patients with HT (mean age; 31.3 ± 11.1 years), and 53 healthy control group (mean age; 29.3 ± 8.5 years) in the current study. The patient group consisted of 52 with euthyroid autoimmune thyroiditis, 31 with subclinical hypothyroidism and 10 with overt hypothyroidism. Serum levels of MIF were higher in patients with overt hypothyroidism (6300.9 ± 2504.3 pg/ml) than the euthyroid patients (3955.2 ± 3013.6 pg/ml) (p = 0.036).
  
  
  CONCLUSIONS
  MIF increases in overt hypothyroidism due to the Hashimoto's thyroiditis. Further investigations are needed to explore the role of MIF in pathogenesis of Hashimoto's thyroiditis.
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Teslime
authors.initials: T
authors.lastname: Ayaz
authors.affiliation: Department of Endocrinology, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Serap Baydur
authors.initials: SB
authors.lastname: Sahin
authors.affiliation: Department of Nephrology, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Osman Zikrullah
authors.initials: OZ
authors.lastname: Sahin
authors.affiliation: Department of Biochemistry, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Medine Cumhur
authors.initials: MC
authors.lastname: Cure
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Sumer
authors.affiliation: Department of Internal Medicine, Recep Tayyip Erdogan University, Faculty of Medicine, Rize, 53100 Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Ilkkilic