title: [Molecular characteristic of duck hepatitis A virus type 1 causing pancreatitis ].
abstract: [OBJECTIVE] We studied the molecular characteristics of the full-length genome of duck hepatitis A virus type 1 causing pancreatitis in Muscovy ducklings. [METHODS] We determined the entire genomic sequence of duck hepatitis A virus type 1 strain MPZJ1206 using reverse transcription polymerase chain reaction assay and analyzed the bioinformatics of the viral genome sequence. [ RESULTS] The genome length of strain MPZJ1206 comprised 7703 bases, with a G + C content of 43.05%. The genome of MPZJ1206 contains a single, long open reading frame encoding a polypeptide of 2249 amino acids, with a genomic orgariization similar to those of other isolates of duck hepatitis A virus type 1. MPZJ1206 is identical with previously isolates by 93. 5% - 99. 6% in nucleotide sequence and 97. 9% - 99. 6% in amino acid sequence and shares genetic distance no more than 7%. Phylogenetic analysis based on genome sequence indicates that MPZJ1206 shares a close genetic relationship with two strains isolated in 2011. [CONCLUSION] Although pathotype caused by MPZJ1206 strain is significantly distinct from those induced by classical isolates of duck hepatitis A virus type 1, the genome of MPZJ1206 shares high homology with those of previous isolates. The change of pathotype may result from an alteration in viral tissue tropism of MPZJ1206.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Fu
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Fu
authors.forename: Longfei
authors.initials: L
authors.lastname: Cheng
authors.forename: Chunhe
authors.initials: C
authors.lastname: Wan
authors.forename: Shaohua
authors.initials: S
authors.lastname: Shi
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Lin
authors.forename: Fang
authors.initials: F
authors.lastname: Lin
mesh_terms: D000818:Animals; D001483:Base Sequence; D004372:Ducks; D016679:Genome, Viral; D004371:Hepatitis Virus, Duck / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010195:Pancreatitis / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins