title: Development and characterization of novel microsatellite markers in Hyptis pectinata (Lamiaceae).
abstract: A microsatellite-enriched library was constructed and a set of 19 SSR markers were developed to characterize a germplasm collection of Hyptis pectinata (L.) Poit., maintained at the Universidade Federal de Sergipe (UFS). Fifteen markers of 19 ranged from moderately to highly polymorphic. A total of 113 alleles were identified, with a mean of 7.52 alleles per locus. The mean HO and HE were 0.582 and 0.657, respectively. The primers developed were efficient tools for accessing the genetic diversity of the germplasm collection analyzed and may also be useful for other studies involving this species and other species in the genus Hyptis.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil afblank@ufs.br.
authors.forename: A F
authors.initials: AF
authors.lastname: Blank
authors.affiliation: Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: A S
authors.initials: AS
authors.lastname: Jesus
authors.affiliation: Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: C P
authors.initials: CP
authors.lastname: Santos
authors.affiliation: Laboratório de Biologia Molecular, Agência Paulista de Tecnologia dos Agronegócios, Piracicaba, SP, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Grando
authors.affiliation: Laboratório de Diversidade Genética e Melhoramento, Departamento Genética e Melhoramento de Plantas, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, SP, Brasil.
authors.forename: J B
authors.initials: JB
authors.lastname: Pinheiro
authors.affiliation: Laboratório de Biologia Molecular, Agência Paulista de Tecnologia dos Agronegócios, Piracicaba, SP, Brasil.
authors.forename: M I
authors.initials: MI
authors.lastname: Zucchi
authors.affiliation: Programa de Pós-Graduação em Biotecnologia de Recursos Naturais, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
authors.forename: M F
authors.initials: MF
authors.lastname: Arrigoni-Blank
mesh_terms: D056426:Genetic Loci; D014644:Genetic Variation; D018745:Genome, Plant; D015698:Genomic Library; D031331:Hyptis / Q000145:classification / Q000235:genetics*; D018895:Microsatellite Repeats; D017422:Sequence Analysis, DNA; D013045:Species Specificity