title: Generation of Cardiomyocytes from Pluripotent Stem Cells.
abstract: The advent of pluripotent stem cells (PSCs) enabled a multitude of studies for modeling the development of diseases and testing pharmaceutical therapeutic potential in vitro. These PSCs have been differentiated to multiple cell types to demonstrate its pluripotent potential, including cardiomyocytes (CMs). However, the efficiency and efficacy of differentiation vary greatly between different cell lines and methods. Here, we describe two different methods for acquiring CMs from human pluripotent lines. One method involves the generation of embryoid bodies, which emulates the natural developmental process, while the other method chemically activates the canonical Wnt signaling pathway to induce a monolayer of cardiac differentiation.
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, MI, Italy.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nakahama
authors.affiliation: Humanitas Clinical and Research Center, Rozzano, MI, Italy. elisa.dipasquale@humanitasresearch.it.|Istituto di Ricerca Genetica e Biomedica, National Research Council of Italy, Milan, Italy. elisa.dipasquale@humanitasresearch.it.|C/O Humanitas Clinical and Research Center, Via Manzoni, 56, 20089, Rozzano, MI, Italy. elisa.dipasquale@humanitasresearch.it.
authors.forename: Elisa
authors.initials: E
authors.lastname: Di Pasquale
mesh_terms: D000577:Amides / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D058732:Embryoid Bodies / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D007797:Laminin / Q000737:chemistry; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D061251:Primary Cell Culture; D011509:Proteoglycans / Q000737:chemistry; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D060449:Wnt Signaling Pathway
chemical_list: D000577:Amides; C473711:Chir 99021; D004338:Drug Combinations; D004791:Enzyme Inhibitors; D036341:Intercellular Signaling Peptides and Proteins; D007797:Laminin; D011509:Proteoglycans; D011725:Pyridines; D011743:Pyrimidines; C059485:matrigel; C108830:Y 27632; D003094:Collagen
keywords: Cardiac differentiation; EB method; Human cardiomyocytes; Monolayer method; Pluripotent stem cells