title: MicroRNA expression in inflamed and noninflamed gingival tissues from Japanese patients.
abstract: Periodontitis is a chronic inflammatory disease caused by specific bacteria and viruses. Local, systemic, and environmental factors affect the rate of disease progression. Immune responses to bacterial products, and the subsequent production of inflammatory cytokines, are crucial in the destruction of periodontal tissue. MicroRNAs (miRNAs) are a class of small RNAs that control various cell processes by negatively regulating protein-coding genes. In this study, we compared miRNA expression in inflamed and noninflamed gingival tissues from Japanese dental patients. Total RNAs were isolated from inflamed and noninflamed gingival tissues. miRNA expression profiles were examined by an miRNA microarray, and the data were analyzed by GeneSpring GX, Ingenuity Pathways Analysis, and the TargetScan databases. Observed miRNA expression levels in inflamed gingiva were confirmed by real-time PCR. The three most overexpressed (by >2.72-fold) miRNAs were hsa-miR-150, hsa-miR-223, and hsa-miR-200b, and the three most underexpressed (by <0.39-fold) miRNAs were hsa-miR-379, hsa-miR-199a-5p, and hsa-miR-214. In IPA analysis, hsa-miR-150, hsa-miR-223, and hsa-miR-200b were associated with inflammatory disease, organismal injury, abnormalities, urological disease, and cancer. The present findings suggest that miRNAs are associated with chronic periodontitis lesions in Japanese.
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry at Matsudo.
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
authors.forename: Sari
authors.initials: S
authors.lastname: Matsui
authors.forename: Ayako
authors.initials: A
authors.lastname: Kato
authors.forename: Liming
authors.initials: L
authors.lastname: Zhou
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nakayama
authors.forename: Hideki
authors.initials: H
authors.lastname: Takai
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D055113:Chronic Periodontitis / Q000235:genetics* / Q000473:pathology; D005347:Fibroblasts / Q000473:pathology; D020869:Gene Expression Profiling; D005881:Gingiva / Q000737:chemistry* / Q000166:cytology; D005891:Gingivitis / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007564:Japan; D035683:MicroRNAs / Q000032:analysis*; D046228:Microarray Analysis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; C533097:MIR150, human; C533896:MIRN200 microRNA, human; C547397:MIRN214 microRNA, human; C525067:MIR223, human; C561022:MIRN379 microRNA, human; C531408:mirn199 microRNA, human