title: Biocompatibility and biodegradation of protein microparticle and film scaffolds made from kafirin (sorghum prolamin protein) subcutaneously implanted in rodent models.
abstract: Kafirin, the sorghum prolamin protein, like its maize homologue zein, can be made into microparticles and films and potentially used as a biomaterial. Zein has good bio- and cyto-compatibility. Kafirin could be advantageous as it is more hydrophobic, more crosslinked, more slowly digested by mammalian proteases than zein and is non-allergenic. The safety and biocompatibility of kafirin implants in two forms was determined in rodent models. One week post subcutaneous injection of kafirin microparticles (size 5-µm diameter) in mice, chronic inflammation, abnormal red blood cells, and gross fibrin formation were observed. This chronic inflammatory response was possibly caused by the release of hydrolysis products such as glutamate during the degradation of the kafirin microparticles. In contrast, films made from kafirin microparticles (50-µm thick, folded into 1 cm(3) ) implanted in rats showed no abnormal inflammatory reactions and were only partially degraded by day 28. The slower degradation of the kafirin films was probably due to their far smaller surface area when compared to kafirin microparticles. Thus, kafirin films appear to have potential as a biomaterial. This study also raises awareness that the form of prolamin based biomaterials, (kafirin and zein) should be considered when assessing the safety of such materials.
authors.affiliation: Institute for Food, Nutrition and Well-Being and Department of Food Science, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa.
authors.forename: Janet
authors.initials: J
authors.lastname: Taylor
authors.affiliation: Institute for Food, Nutrition and Well-Being and Department of Food Science, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa.|Department of Dairy and Food Science and Technology, Egerton University, Nakuru, Kenya.
authors.forename: Joseph O
authors.initials: JO
authors.lastname: Anyango
authors.affiliation: Department of Immunology and Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
authors.forename: Marnie
authors.initials: M
authors.lastname: Potgieter
authors.affiliation: Department of Immunology and Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
authors.forename: Karlien
authors.initials: K
authors.lastname: Kallmeyer
authors.affiliation: Biomedical Research Centre, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa.
authors.forename: Vinny
authors.initials: V
authors.lastname: Naidoo
authors.affiliation: Department of Immunology and Institute for Cellular and Molecular Medicine, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
authors.forename: Michael S
authors.initials: MS
authors.lastname: Pepper
authors.affiliation: Institute for Food, Nutrition and Well-Being and Department of Food Science, University of Pretoria, Private Bag X20, Hatfield, 0028, South Africa.
authors.forename: John R N
authors.initials: JR
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D007279:Injections, Subcutaneous; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010940:Plant Proteins / Q000008:administration & dosage*; D011506:Proteins / Q000378:metabolism*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D010940:Plant Proteins; D011506:Proteins; C080615:kafirin protein, Sorghum bicolor
keywords: biocompatibility; film; histology; kafirin; microparticle