title: [The influence of miR-16 on proliferation and angiogenesis of U87MG in vivo].
abstract: OBJECTIVE
  To explore the proliferation inhibition of miR-16 to U87MG in vivo.
  
  
  METHODS
  The miR-16 over-expression U87MG cells were obtained by infecting U87MG with lentivirus-hsa-GFP-miR-16. The models of subcutaneous transplantation and orthotopic brain glioma were obtained by injecting cells into flank and brains of nude mouse separately. Untransfected U87MG, negative control U87MG and miR-16 over-expression U87MG were separatively cultured and injected into brains of balb/c-nu mice for establishing an orthotopic model of brain glioma. Tumor growth curve was calculated for subcutaneous tumor. The brains were sectioned and stained with hematoxylin & eosin (H&E) and immunohistochemistry for CD31 and cyclinD1.
  
  
  RESULTS
  The miR-16 over-expression U87MG cells formed no apparent tumors while the negative control U87MG grew into large tumors. HE stain of brains showed that gliomas of miR-16 over-expression U87MG (0.11 ± 0.04 mm³) were smaller than those of negative control U87MG (6.50 ± 1.41 mm³) and untransfected (6.41 ± 0.91 mm³) in murine brains. Immunohistochemistry showed that cyclinD1 staining was less in gliomas of miR-16 over-expression U87MG than the other two groups. CD31 immunohistochemistry also yielded the similar results. The mean microvascular density (MVD) of gliomas of miR-16 over-expression U87MG (4.50 ± 1.58/× 200) was smaller than negative control (30.40 ± 6.57/× 200) and untransfected (29.40 ± 4.93/× 200) (P < 0.05).
  
  
  CONCLUSIONS
  miR-16 can significantly inhibit the in vivo growth of U87MG glioma. This is probably the results of cell proliferation inhibition and angiogenesis suppression.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Nu
authors.initials: N
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Zhou
authors.forename: Lisheng
authors.initials: L
authors.lastname: Yu
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Hansong
authors.initials: H
authors.lastname: Sheng
authors.affiliation: Email: maodewang@163.com.
authors.forename: Maode
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D001932:Brain Neoplasms; D049109:Cell Proliferation; D005910:Glioma; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs; D009389:Neovascularization, Pathologic
chemical_list: D035683:MicroRNAs