title: [Anatomopathological findings during development of diabetic cardiomyopathy in rats].
abstract: BACKGROUND
  The diabetic cardiomyopathy occurs in both type 1 and type 2 diabetes mellitus. Hyperglycemia and associated metabolic changes participate in the pathogenesis of this disease.
  
  
  OBJECTIVE
  To characterizes various pathological changes occurring during the development of diabetic cardiomyopathy in rats.
  
  
  METHODS
  Diabetic rats were used for streptozotocin administration. At 7, 14, 21 and 30 days after toxic administration, the heart was obtained and placed in a Hartman solution and 4% p-formaldehyde. Five-micrometer thick sections were stained with hematoxylin-eosin, Masson trichrome and immunocytochemistry using anti-β-tubulin antibody.
  
  
  RESULTS
  At 14 days after application of streptozotocin, dilated sinusoids with endothelial lining in the myocardium and collagen deposits in the cardiac interstitium and between the Purkinje fibers were observed. At 21 days there was a slight decrease of the arteriolar lumen due to hyperplasia of the medial layer. It is important to note that cardiac sinusoids as well as collagen deposits became more evident at 30 day of the study, as well as a major derangement of the microtubular system of the cardiomyocytes.
  
  
  CONCLUSIONS
  Cardiac sinusoids representing fetal vascular pattern and interstitial fibrosis in the myocardium and the microtubular derangement of cardiomyocytes support the fact that the pathophysiological mechanism of diabetic cardiomyopathy begins in the coronary microcirculation due to changes in cardiac metabolism, contributing to the development of myocardial dysfunction in diabetes.
authors.affiliation: Unidad de Investigación Biomolecular del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico DF, Mexico. willisga@prodigy.net.mx.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Manjarrez-Gutiérrez
authors.affiliation: Departamento de Patología, Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico DF, Mexico.
authors.forename: Victor
authors.initials: V
authors.lastname: Hernández-Chávez
authors.affiliation: Unidad de Investigación Biomolecular del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico DF, Mexico.
authors.forename: Teresa
authors.initials: T
authors.lastname: Neri-Gómez
authors.affiliation: Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Naciona, Mexico DF, Mexico.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Boyzo-Montes de Oca
authors.affiliation: Unidad de Investigación Biomolecular del Hospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico DF, Mexico.
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Mondragón-Herrera
authors.affiliation: Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Naciona, Mexico DF, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Hernández-Rodríguez
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D002196:Capillaries / Q000473:pathology; D009202:Cardiomyopathies / Q000209:etiology / Q000473:pathology*; D003094:Collagen / Q000032:analysis; D003326:Coronary Circulation; D003599:Cytoskeleton / Q000648:ultrastructure; D048909:Diabetes Complications / Q000473:pathology*; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D003925:Diabetic Angiopathies / Q000473:pathology; D004435:Eating; D005355:Fibrosis; D008297:Male; D008833:Microcirculation; D008870:Microtubules / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014404:Tubulin / Q000032:analysis
chemical_list: D001786:Blood Glucose; D014404:Tubulin; D013311:Streptozocin; D003094:Collagen
keywords: Cardiomyopathy; cardiac sinusoids; collagen; colágena; diabetes mellitus; miocardiopatía; sinusoides cardiacos