title: Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil.
abstract: Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study we isolated the bacteria from diesel contaminated soil and screened them for diesel biodegradation capacity. One monoculture isolate identified by 16S rRNA gene sequence analysis to be Acinetobacter baumannii was further studied for diesel oil biodegradation. The effects of various culture parameters (pH, temperature, NaCl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemical surfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. Optimal diesel oil biodegradation by A. baumanii occurred at initial pH 7, 35°C and initial hydrocarbon concentration at 4%. The biodegradation products under optimal cultural conditions were analyzed by GC-MS. The present study suggests that A. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated with diesel oil.
authors.affiliation: Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India.
authors.forename: Nandhini
authors.initials: N
authors.lastname: Palanisamy
authors.affiliation: Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India.
authors.forename: Jayaprakash
authors.initials: J
authors.lastname: Ramya
authors.affiliation: Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India.
authors.forename: Srilakshman
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India.
authors.forename: Ns
authors.initials: N
authors.lastname: Vasanthi
authors.affiliation: CeNTAB, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401 Tamil Nadu India.
authors.forename: Preethy
authors.initials: P
authors.lastname: Chandran
authors.affiliation: Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu India ; CeNTAB, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401 Tamil Nadu India.
authors.forename: Sudheer
authors.initials: S
authors.lastname: Khan
keywords: Acinetobacter baumannii; Biodegradation; Diesel oil; GC-MS analysis; Parameter optimization