title: Conformal nanopatterning of extracellular matrix proteins onto topographically complex surfaces.
abstract: Our Patterning on Topography (PoT) printing technique enables fibronectin, laminin and other proteins to be applied to biomaterial surfaces in complex geometries that are inaccessible using traditional soft lithography techniques. Engineering combinatorial surfaces that integrate topographical and biochemical micropatterns enhances control of the biotic-abiotic interface. Here, we used this method to understand cardiomyocyte response to competing physical and chemical cues in the microenvironment.
authors.affiliation: 1] Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA. [2] School of Biological Science and Medical Engineering, Beihang University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Jallerat
authors.affiliation: Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Szymanski
authors.affiliation: 1] Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA. [2] Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, USA.
authors.forename: Adam W
authors.identifier: 0000000333385456
authors.initials: AW
authors.lastname: Feinberg
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D002642:Chick Embryo; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D016326:Extracellular Matrix Proteins / Q000737:chemistry*; D006321:Heart / Q000196:embryology; D032383:Myocytes, Cardiac / Q000166:cytology; D036103:Nanotechnology / Q000379:methods*; D011327:Printing; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D016326:Extracellular Matrix Proteins