title: A new apparatus for standardized rat kidney biopsy.
abstract: Survival biopsies are frequently applied in rat kidney disease models, but several drawbacks such as surgical kidney trauma, bleeding risk and variable loss of kidney tissue are still unsolved. Therefore, we developed an easy-to-use core biopsy instrument and evaluated whether two consecutive kidney biopsies within the same kidney can be carried out in a standardized manner. On day 0, 18 Lewis rats underwent a right nephrectomy and 9 of these rats a subsequent first biopsy of the left kidney (Bx group). 9 control rats had a sham biopsy of the left kidney (Ctrl group). On day 7, a second kidney biopsy/sham biopsy was performed. On day 42, all animals were sacrificed and their kidneys were removed for histology. Biopsy cylinders contained 57±28 glomeruli per transversal section, representing an adequate sample size. PAS staining showed that the biopsy depth was limited to the renal cortex whereas surgical tissue damage was limited to the area immediately adjacent to the taken biopsy cylinder. On day 42, the reduction of functional renal mass after two biopsies was only 5.2% and no differences of body weight, blood pressure, proteinuria, serum creatinine, glomerulosclerosis, interstitial fibrosis or number of ED-1 positive macrophages were found between both groups. In summary, our apparatus offers a safe method to perform repetitive kidney biopsies with minimal trauma and sufficient sample size and quality even in experimental disease models restricted to one single kidney.
authors.affiliation: Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany.
authors.forename: Holger
authors.initials: H
authors.lastname: Schirutschke
authors.affiliation: Institute for Mechanical Engineering, Technische Universität Dresden, Dresden, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Gladrow
authors.affiliation: Institute for Mechanical Engineering, Technische Universität Dresden, Dresden, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Norkus
authors.affiliation: Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany.
authors.forename: Simon Paul
authors.initials: SP
authors.lastname: Parmentier
authors.affiliation: Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Hohenstein
authors.affiliation: Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus at the Technische Universität Dresden, Dresden, Germany.
authors.forename: Christian P M
authors.initials: CP
authors.lastname: Hugo
mesh_terms: D000818:Animals; D001706:Biopsy / Q000295:instrumentation* / Q000592:standards; D004195:Disease Models, Animal; D007672:Kidney Cortex / Q000473:pathology*; D008297:Male; D009392:Nephrectomy; D051381:Rats; D011917:Rats, Inbred Lew