title: [Preparation of herpetin lyophilized liposome and evaluation on its safety and pharmacodynamics].
abstract: In this study, the herpetin (HPT) lyophilized liposome was prepared, and its saftey and pharmacodynamics were evaluated. HPT lyophilized liposome was prepared by thin-film ultrasonication method. The lyoprotectant was optimized using particle size and encapsulation efficiency as indexes. Then, the influencing factors of HPT lyophilized liposome were investigated. In addition, preliminary safety and therapy efficiency of HPT lyophilized liposome to liver injury induced by CCl4 in the mice. The optimal lyoprotectant was 5% sucrose plus 5% lactose and the dispersed HPT lyophilized liposomes were spherical with the mean diameter of (107.0 ± 1.2) nm and the mean encapsulation efficiency of (99.7 ± 0.50)%. The lyophilized powder was sensitive to temperature, humidity and illumination. None of hemolysis, hemagglutination and vein irritation was observed after intravenous injection of HPT lyophilized liposomes into rabbits. HPT lyophilized liposome showed obviously therapy efficiency to liver injury induced by CCl4 in the mice. The improvements of ALT, AST and ALP were better than that in HPT free drug. The obtained HPT lyophilized liposome met the standard of CP with fine particle size and encapsulation efficiency after dispersion. The HPT lyophilized liposome showed good safety and enhanced the treatment efficacy of HPT. The HPT lyophilized liposome should be stored in low temperature, sealed condition far away from light.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Tan
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Pu-Yang
authors.initials: PY
authors.lastname: Gong
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical; D019662:Cucurbitaceae / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D004353:Drug Evaluation, Preclinical; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000009:adverse effects* / Q000737:chemistry / Q000493:pharmacokinetics*; D008081:Liposomes / Q000737:chemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits
chemical_list: D004337:Drug Carriers; D004365:Drugs, Chinese Herbal; D008081:Liposomes