title: Single-chain semiconducting polymer dots.
abstract: This work describes the preparation and validation of single-chain semiconducting polymer dots (sPdots), which were generated using a method based on surface immobilization, washing, and cleavage. The sPdots have an ultrasmall size of ∼3.0 nm as determined by atomic force microscopy, a size that is consistent with the anticipated diameter calculated from the molecular weight of the single-chain semiconducting polymer. sPdots should find use in biology and medicine as a new class of fluorescent probes. The FRET assay this work presents is a simple and rapid test to ensure methods developed for preparing sPdot indeed produced single-chain Pdots as designed.
authors.affiliation: Department of Chemistry and ‡Department of Material Science and Engineering, University of Washington , Seattle, Washington 98195, United States.
authors.forename: Fangmao
authors.initials: F
authors.lastname: Ye
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Changfeng
authors.initials: C
authors.lastname: Wu
authors.forename: Xuanjun
authors.initials: X
authors.lastname: Zhang
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Rong
authors.forename: Miqin
authors.initials: M
authors.lastname: Zhang
authors.forename: Daniel T
authors.initials: DT
authors.lastname: Chiu
mesh_terms: D031541:Fluorescence Resonance Energy Transfer; D018625:Microscopy, Atomic Force; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D012666:Semiconductors; D013499:Surface Properties
chemical_list: D011108:Polymers