title: Protein profiling and tps23 induction in different maize lines in response to methyl jasmonate treatment and Diabrotica virgifera infestation.
abstract: Plant responses to herbivore insects involve direct and indirect defense with the production of signal molecules including jasmonic acid (JA) and its derivatives (e.g. methyl jasmonate, MeJA). In maize (Zea mays), root feeding by Diabrotica virgifera larvae activates an indirect defense mechanism, through enthomopathogenic nematodes that are recruited after Terpene Synthase 23 (tps23) upregulation and (E)-β-caryophyllene root emission. In order to gain insight into the correlation between JA signaling and response to Diabrotica attack, we analyzed tps23 expression and protein profiles in maize roots in response to MeJA treatment and insect infestation. Similar to herbivore feeding, MeJA treatment was found to increase tps23 transcript accumulation, with consistent variations for both treatments in maize lines differing in (E)-β-caryophyllene production. Analysis of root protein profiles showed specific alterations leading to the identification of three proteins that were induced by MeJA treatment. We focused on a peroxidase-like protein (Px-like) showing that the corresponding transcripts accumulated in all tested lines. Results show that exogenous application of MeJA upregulates tps23 expression and specifically alters protein patterns in maize roots. Parallel effects on tps23 transcript accumulation were observed upon hormone exposure and insect infestation in different maize lines. In contrast, Px-like transcript profiling showed differences between treatments. These results support the possible involvement of MeJA in mediating the upregulation of tps23 in response to Diabrotica attack.
authors.affiliation: Istituto di Biologia e Biotecnologia Agraria Consiglio Nazionale delle Ricerche IBBA-CNR, via Einstein, 26900 Lodi, Italy; Biotrack S.r.l., Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy. Electronic address: e.capra@ibba.cnr.it.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Capra
authors.affiliation: Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Colombi
authors.affiliation: Biotrack S.r.l., Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy.
authors.forename: Pamela
authors.initials: P
authors.lastname: De Poli
authors.affiliation: Università degli Studi di Milano, DiSAA, via Celoria 2, 20133 Milan, Italy.
authors.forename: Fabio Francesco
authors.initials: FF
authors.lastname: Nocito
authors.affiliation: Università degli Studi di Milano, DiSAA, via Celoria 2, 20133 Milan, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Cocucci
authors.affiliation: Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Vecchietti
authors.affiliation: Istituto di Agronomia, Genetica e Coltivazioni erbacee, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Marocco
authors.affiliation: Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy; Agroalimentare Sud SpA, Loc. S. Nicola, 85025 Melfi, PZ, Italy.
authors.forename: Maria Rosaria
authors.initials: MR
authors.lastname: Stile
authors.affiliation: Parco Tecnologico Padano, via Einstein, 26900 Lodi, Italy; Università degli Studi di Milano, DiSAA, via Celoria 2, 20133 Milan, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Rossini
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001517:Coleoptera / Q000502:physiology*; D003517:Cyclopentanes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant; D060434:Herbivory; D007814:Larva; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000081223:Polycyclic Sesquiterpenes; D020543:Proteome; D040901:Proteomics; D012717:Sesquiterpenes / Q000378:metabolism; D013045:Species Specificity; D003313:Zea mays / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000081223:Polycyclic Sesquiterpenes; D020543:Proteome; D012717:Sesquiterpenes; C072239:methyl jasmonate; C024714:caryophyllene; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: Jasmonic acid; Protein profile; Terpene synthase; Western corn rootworm; Zea mays