title: [microRNA-126 delivered by microparticles mediates intercellular signal transmission].
abstract: OBJECTIVE
  To investigate the role of microRNAs (miRNAs) in mediating intercellular signaling.
  
  
  METHODS
  Microparticles (MP) from HUVEC and 293T were isolated by sequential centrifugation. THP-1 was co-cultured with microparticles. And then the migration of THP-1 was measured by transwell. real-time PCR and Western blotting were used to study the related mechanisms.
  
  
  RESULTS
  Compared with the microparticles from 293T, MP from HUVEC could promote the migration of monocytes (P<0.05) and upregulate the expression of CXCR4 mRNA and protein (P<0.05). MiRNA-126 deficient MP could downregulate the migration of monocytes (P<0.05) and the expression of CXCR4 mRNA and protein (P<0.05) compared with miRNA-126 abundant MP.
  
  
  CONCLUSIONS
  Microparticles from HUVEC could promote the migration of monocytes. As carriers, microparticles could mediate intercellular signaling.
authors.affiliation: Department of Cardiology, Peking University People's Hospital, Beijing 100044.
authors.forename: Q
authors.initials: Q
authors.lastname: Geng
authors.affiliation: Department of Cardiology, Peking University People's Hospital, Beijing 100044.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Cardiology, Peking University People's Hospital, Beijing 100044.
authors.forename: J Y
authors.initials: JY
authors.lastname: Ren
authors.affiliation: Department of Cardiology, Peking University People's Hospital, Beijing 100044.
authors.forename: J X
authors.initials: JX
authors.lastname: Song
authors.affiliation: Department of Cardiology, Peking University People's Hospital, Beijing 100044.
authors.forename: S F
authors.initials: SF
authors.lastname: Li
mesh_terms: D002460:Cell Line; D018920:Coculture Techniques; D015536:Down-Regulation; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology*; D012333:RNA, Messenger; D019718:Receptors, CXCR4 / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: C527276:CXCR4 protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D019718:Receptors, CXCR4