title: Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC.
abstract: Pluripotent Embryonic Stem cell (ESC) lines can be derived from a variety of sources. Mouse lines derived from the early blastocyst and from primordial germ cells (PGCs) can contribute to all somatic lineages and to the germ line, whereas cells from slightly later embryos (EpiSC) no longer contribute to the germ line. In chick, pluripotent ESCs can be obtained from PGCs and from early blastoderms. Established PGC lines and freshly isolated blastodermal cells (cBC) can contribute to both germinal and somatic lineages but established lines from the former (cESC) can only produce somatic cell types. For this reason, cESCs are often considered to be equivalent to mouse EpiSC. To define these cell types more rigorously, we have performed comparative microarray analysis to describe a transcriptomic profile specific for each cell type. This is validated by real time RT-PCR and in situ hybridisation. We find that both cES and cBC cells express classic pluripotency-related genes (including cPOUV/OCT4, NANOG, SOX2/3, KLF2 and SALL4), whereas expression of DAZL, DND1, DDX4 and PIWIL1 defines a molecular signature for germ cells. Surprisingly, contrary to the prevailing view, our results also suggest that cES cells resemble mouse ES cells more closely than mouse EpiSC.
authors.affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Jean
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Nidia M M
authors.initials: NM
authors.lastname: Oliveira
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Sittipon
authors.initials: S
authors.lastname: Intarapat
authors.affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Fuet
authors.affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Mazoyer
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Irene
authors.initials: I
authors.lastname: De Almeida
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: Trevers
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Sharon
authors.initials: S
authors.lastname: Boast
authors.affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Aubel
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Federica
authors.initials: F
authors.lastname: Bertocchini
authors.affiliation: Department of Cell and Developmental Biology, University College London, London, UK.
authors.forename: Claudio D
authors.initials: CD
authors.lastname: Stern
authors.affiliation: INSERM, U846, Stem Cell and Brain Research Institute, Bron, France; INRA, USC1361, Bron, France; Université de Lyon, Lyon 1, UMR S 846, Lyon, France. Electronic address: bertrand.pain@inserm.fr.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Pain
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002645:Chickens; D016000:Cluster Analysis; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D020869:Gene Expression Profiling; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D017403:In Situ Hybridization; D051379:Mice; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D025341:Principal Component Analysis; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; C508515:DAZL protein, mouse; C500862:Dnd1 protein, mouse; D009363:Neoplasm Proteins; C448522:Piwil1 protein, mouse; D016601:RNA-Binding Proteins; D014157:Transcription Factors; C090864:Ddx4 protein, mouse; D053487:DEAD-box RNA Helicases