title: Preparation and evaluation of a novel hybrid monolithic column based on pentafluorobenzyl imidazolium bromide ionic liquid.
abstract: To develop a novel hybrid monolithic column based on pentafluorobenzyl imidazolium bromide ionic liquid, a new ionic liquid monomer was synthesized from 1-vinylimidazole and pentafluorobenzyl bromide. By employing a facile one-step copolymerization of polyhedral-oligomeric-silsesquioxane-type (POSS) cross-linking agent and the home-made ionic liquid monomer, the hybrid monolithic columns were in situ fabricated in fused-silica capillary. The morphology of monolithic column was characterized by scanning electron microscope (SEM) and the chemical composition was confirmed by Fourier-transform infrared spectroscopy (FT-IR) and elemental analysis. Excellent mechanical stability and slight swelling propensity were exhibited which was ascribed to the rigid hybrid monolithic skeleton. Reproducibility results of run-to-run, column-to-column, batch-to-batch and day-to-day were investigated and the RSDs were less than 0.46%, 1.84%, 3.96% and 3.17%, respectively. The mixed-mode retention mechanism with hydrophobic interaction, π-π stacking, ion-exchange, electrostatic interaction and dipole-dipole interaction was explored systematically using analytes with different structure types. Satisfied separation capability and column efficiency were achieved for the analysis of small molecular compounds such as alkylbenzenes, polycyclic aromatic hydrocarbons, nucleosides and halogenated compounds.
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Yuanhong
authors.initials: Y
authors.lastname: Shan
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: shixianzhe@dicp.ac.cn.
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Shi
authors.affiliation: Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xugw@dicp.ac.cn.
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006846:Hydrocarbons, Halogenated / Q000302:isolation & purification; D057927:Hydrophobic and Hydrophilic Interactions; D007093:Imidazoles / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D009705:Nucleosides / Q000302:isolation & purification; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D006846:Hydrocarbons, Halogenated; D007093:Imidazoles; D052578:Ionic Liquids; D009705:Nucleosides; D014753:Vinyl Compounds; D012822:Silicon Dioxide
keywords: Capillary liquid chromatography; Hybrid monolithic column; Mixed-mode retention; Pentafluorobenzyl imidazolium bromide ionic liquid; Polyhedral oligomeric silsesquioxane