title: Biochemical characterization of the POM-1 metallo-β-lactamase from Pseudomonas otitidis.
abstract: The POM-1 metallo-β-lactamase is a subclass B3 resident enzyme produced by Pseudomonas otitidis, a pathogen causing otic infections. The enzyme was overproduced in Escherichia coli BL21(DE3), purified by chromatography, and subjected to structural and functional analysis. The purified POM-1 is a tetrameric enzyme of broad substrate specificity with higher catalytic activities with penicillins and carbapenems than with cephalosporins.
authors.affiliation: Dipartimento di Biotecnologie Mediche, Università di Siena, Siena, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Borgianni
authors.affiliation: Dipartimento di Biotecnologie Mediche, Università di Siena, Siena, Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: De Luca
authors.affiliation: Dipartimento di Biologia, Università di Roma Tor Vergata, Rome, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Thaller
authors.affiliation: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, South Korea.
authors.forename: Yunsop
authors.initials: Y
authors.lastname: Chong
authors.affiliation: Dipartimento di Biotecnologie Mediche, Università di Siena, Siena, Italy Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy Clinical Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, Italy.
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Rossolini
authors.affiliation: Dipartimento di Biotecnologie Mediche, Università di Siena, Siena, Italy jddocquier@unisi.it.
authors.forename: Jean-Denis
authors.initials: JD
authors.lastname: Docquier
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism* / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology; D002384:Catalysis; D002511:Cephalosporins / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism*; D001618:beta-Lactamases / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D002511:Cephalosporins; D010406:Penicillins; D001618:beta-Lactamases