title: Environmental parameters influence on the dynamics of total and pathogenic Vibrio parahaemolyticus densities in Crassostrea virginica harvested from Mexico's Gulf coast.
abstract: The influence of environmental parameters on the total and pathogenic Vibrio parahaemolyticus seasonal densities in American oysters (Crassostrea virginica) was evaluated for 1 year. Harvesting site A yielded the highest mean densities of V. parahaemolyticus tlh+, tdh+/trh-, tdh-/trh+ and tdh+/trh+ during spring season at 2.57, 1.74, 0.36, and -0.40 log10 MPN/g, respectively, and tdh+/orf8+ during winter season (0.90 log10 MPN/g). V. parahaemolyticus tlh+ densities were associated to salinity (R(2)=0.372, P<0.022), tdh+/trh+ to turbidity (R(2)=0.597, P<0.035), and orf8+ to temperature, salinity, and pH (R(2)=0.964, P<0.001). The exposure to salinity and temperature conditions during winter and spring seasons regulated the dynamics of V. parahaemolyticus harboring potentially pathogenic genotypes within the oyster. The adaptive response of V. parahaemolyticus to seasonal environmental changes may lead to an increase in survival and virulence, threatening the seafood safety and increasing the risk of illness.
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico.
authors.forename: Karla M
authors.initials: KM
authors.lastname: López-Hernández
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico. Electronic address: vpardio@yahoo.com.mx.
authors.forename: Violeta T
authors.initials: VT
authors.lastname: Pardío-Sedas
authors.affiliation: Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California CP 22860, Mexico.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lizárraga-Partida
authors.affiliation: Dirección General de Desarrollo Académico, Edificio Central-Rectoría, Universidad Autónoma de Yucatán, Mérida, Yucatán CP 97000, Mexico.
authors.forename: José de J
authors.initials: Jde J
authors.lastname: Williams
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Martínez-Herrera
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico.
authors.forename: Argel
authors.initials: A
authors.lastname: Flores-Primo
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico.
authors.forename: Roxana
authors.initials: R
authors.lastname: Uscanga-Serrano
authors.affiliation: Facultad de Medicina Veterinaria y Zootecnia, Universidad Veracruzana, Av. Miguel Ángel de Quevedo s/n esq. Yañez, Col. Unidad Veracruzana, Veracruz CP 91710, Mexico.
authors.forename: Karla
authors.initials: K
authors.lastname: Rendón-Castro
mesh_terms: D000818:Animals; D049896:Crassostrea / Q000254:growth & development / Q000382:microbiology*; D004784:Environmental Monitoring; D008800:Mexico; D010125:Ostreidae / Q000254:growth & development / Q000382:microbiology*; D025341:Principal Component Analysis; D054712:Salinity; D012621:Seasons; D012623:Seawater / Q000737:chemistry*; D012758:Shellfish / Q000382:microbiology*; D013696:Temperature; D014736:Vibrio parahaemolyticus / Q000254:growth & development / Q000302:isolation & purification* / Q000472:pathogenicity; D014774:Virulence
keywords: Environmental parameters; Oyster; Pathogenic genes; Vibrio parahaemolyticus