title: The secreted proteins of Achlya hypogyna and Thraustotheca clavata identify the ancestral oomycete secretome and reveal gene acquisitions by horizontal gene transfer.
abstract: Saprotrophic and parasitic microorganisms secrete proteins into the environment to breakdown macromolecules and obtain nutrients. The molecules secreted are collectively termed the "secretome" and the composition and function of this set of proteins varies depending on the ecology, life cycle, and environment of an organism. Beyond the function of nutrient acquisition, parasitic lineages must also secrete molecules to manipulate their host. Here, we use a combination of de novo genome and transcriptome sequencing and bioinformatic identification of signal peptides to identify the putative secreted proteome of two oomycetes, the facultative parasite Achlya hypogyna and free-living Thraustotheca clavata. By comparing the secretomes of these saprolegnialean oomycetes with that of eight other oomycetes, we were able to characterize the evolution of this protein set across the oomycete clade. These species span the last common ancestor of the two major oomycete families allowing us to identify the ancestral secretome. This putative ancestral secretome consists of at least 84 gene families. Only 11 of these gene families are conserved across all 10 secretomes analyzed and the two major branches in the oomycete radiation. Notably, we have identified expressed elicitin-like effector genes in the saprotrophic decomposer, T. clavata. Phylogenetic analyses show six novel horizontal gene transfers to the oomycete secretome from bacterial and fungal donor lineages, four of which are specific to the Saprolegnialeans. Comparisons between free-living and pathogenic taxa highlight the functional changes of oomycete secretomes associated with shifts in lifestyle.
authors.affiliation: Department of Biological Sciences, The University of Rhode Island Department of Biological Sciences, The University of Maryland, College Park imisner@umd.edu.
authors.forename: Ian
authors.initials: I
authors.lastname: Misner
authors.affiliation: Department of Biological Sciences, The University of Rhode Island.
authors.forename: Nic
authors.initials: N
authors.lastname: Blouin
authors.affiliation: Biosciences, University of Exeter, United Kingdom.
authors.forename: Guy
authors.initials: G
authors.lastname: Leonard
authors.affiliation: Biosciences, University of Exeter, United Kingdom Integrated Microbial Biodiversity Program, Canadian Institute for Advanced Research, Toronto, Ontario, Canada.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Richards
authors.affiliation: Department of Biological Sciences, The University of Rhode Island.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Lane
mesh_terms: D044743:Achlya / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D022761:Gene Transfer, Horizontal; D016681:Genome, Fungal; D010802:Phylogeny; D020543:Proteome
chemical_list: D005656:Fungal Proteins; D020543:Proteome
keywords: comparative genomics; evolution; horizontal gene transfer; oomycete; osmotrophy