title: PINK1 kinase catalytic activity is regulated by phosphorylation on serines 228 and 402.
abstract: Mutations in the PINK1 gene cause early-onset recessive Parkinson disease. PINK1 is a mitochondrially targeted kinase that regulates multiple aspects of mitochondrial biology, from oxidative phosphorylation to mitochondrial clearance. PINK1 itself is also phosphorylated, and this might be linked to the regulation of its multiple activities. Here we systematically analyze four previously identified phosphorylation sites in PINK1 for their role in autophosphorylation, substrate phosphorylation, and mitophagy. Our data indicate that two of these sites, Ser-228 and Ser-402, are autophosphorylated on truncated PINK1 but not on full-length PINK1, suggesting that the N terminus has an inhibitory effect on phosphorylation. We furthermore establish that phosphorylation of these PINK1 residues regulates the phosphorylation of the substrates Parkin and Ubiquitin. Especially Ser-402 phosphorylation appears to be important for PINK1 function because it is involved in Parkin recruitment and the induction of mitophagy. Finally, we identify Thr-313 as a residue that is critical for PINK1 catalytic activity, but, in contrast to previous reports, we find no evidence that this activity is regulated by phosphorylation. These data clarify the regulation of PINK1 through multisite phosphorylation.
authors.affiliation: From the Center for the Biology of Disease, Flemish Institute for Biotechnology (VIB) and Center for Human Genetics, Leuven Institute for Neurodegenerative Disorders and University Hospitals Leuven, University of Leuven, 3000 Leuven, Belgium and.
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Aerts
authors.affiliation: From the Center for the Biology of Disease, Flemish Institute for Biotechnology (VIB) and Center for Human Genetics, Leuven Institute for Neurodegenerative Disorders and University Hospitals Leuven, University of Leuven, 3000 Leuven, Belgium and.
authors.forename: Katleen
authors.initials: K
authors.lastname: Craessaerts
authors.affiliation: From the Center for the Biology of Disease, Flemish Institute for Biotechnology (VIB) and Center for Human Genetics, Leuven Institute for Neurodegenerative Disorders and University Hospitals Leuven, University of Leuven, 3000 Leuven, Belgium and the University College London, Institute of Neurology, Queen Square, London WC1N 3BG, United Kingdom.
authors.forename: Bart
authors.initials: B
authors.lastname: De Strooper
authors.affiliation: From the Center for the Biology of Disease, Flemish Institute for Biotechnology (VIB) and Center for Human Genetics, Leuven Institute for Neurodegenerative Disorders and University Hospitals Leuven, University of Leuven, 3000 Leuven, Belgium and Vanessa.Morais@cme.vib-kuleuven.be.
authors.forename: Vanessa A
authors.initials: VA
authors.lastname: Morais
mesh_terms: D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D012694:Serine / Q000737:chemistry / Q000378:metabolism*; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D011494:Protein Kinases; D012694:Serine; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases; D000099202:PTEN-Induced Putative Kinase; C111567:parkin protein
keywords: Mitochondria; Neurodegenerative Disease; PTEN-induced Putative Kinase 1 (PINK1); Parkinson Disease; Posttranslational Modification (PTM); Protein Phosphorylation; Serine/Threonine Protein Kinase