title: Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi.
abstract: In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector Anopheles stephensi as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Epis
authors.affiliation: Department of Environmental Biology, University "La Sapienza" of Rome, Rome, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Porretta
authors.affiliation: Department of Environmental Biology, University "La Sapienza" of Rome, Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mastrantonio
authors.affiliation: Department of Environmental Biology, University "La Sapienza" of Rome, Rome, Italy.
authors.forename: Sandra
authors.initials: S
authors.lastname: Urbanelli
authors.affiliation: Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Sassera
authors.affiliation: Department of Biology and Biotechnology, University of Pavia, Pavia, Italy.
authors.forename: Leone
authors.initials: L
authors.lastname: De Marco
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Mereghetti
authors.affiliation: Department of Agricultural and Environmental Sciences, University of Milan, Milan, Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Montagna
authors.affiliation: School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Ricci
authors.affiliation: School of Bioscience and Veterinary Medicine, University of Camerino, Camerino, Italy.
authors.forename: Guido
authors.initials: G
authors.lastname: Favia
authors.affiliation: Department of Veterinary Science and Public Health, University of Milan, Milan, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Bandi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D026023:Permethrin / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
chemical_list: D018528:ATP-Binding Cassette Transporters; D019476:Insect Proteins; D007306:Insecticides; D026023:Permethrin