title: Hydrolysate from eggshell membrane ameliorates intestinal inflammation in mice.
abstract: Inflammatory bowel diseases (IBD) comprises of ulcerative colitis (UC) and Cohn's disease (CD) as two main idiopathic pathologies resulting in immunologically mediated chronic inflammatory conditions. Several bioactive peptides and hydro lysates from natural sources have now been tested in animal models of human diseases for potential anti-inflammatory effects. Eggshell membrane (ESM) is a well-known natural bioactive material. In this study, we aim to study the anti-inflammatory activity of ESM hydro lysate (AL-PS) in vitro and in vivo. In vitro, AL-PS was shown to inhibit pro-inflammatory cytokine IL-8 secretion. In vivo treatment with AL-PS was shown to reduce dextran sodium sulphate (DSS)-induced weight loss, clinical signs of colitis and secretion of interleukin (IL)-6 (p < 0.05). In addition, treatment with AL-PS also attenuated the severity of intestinal inflammation via down-regulation of IL-10 an anti-inflammatory cytokine. This validates potential benefits of AL-PS as a novel preventative target molecule for treatment of IBD.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. pbabu@uoguelph.ca.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada. pbabu@uoguelph.ca.
authors.forename: Prithy
authors.initials: P
authors.lastname: Rupa
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. bjiang@jiangnan.edu.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada. ymine@uoguelph.ca.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Mine
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D001688:Biological Products / Q000008:administration & dosage / Q000494:pharmacology*; D002460:Cell Line; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D004195:Disease Models, Animal; D004527:Egg Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006867:Hydrolases / Q000008:administration & dosage / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects* / Q000473:pathology*; D051379:Mice
chemical_list: D000893:Anti-Inflammatory Agents; D051017:Apoptosis Regulatory Proteins; D001688:Biological Products; D016207:Cytokines; D004527:Egg Proteins; D018836:Inflammation Mediators; C015753:chorion proteins; D006867:Hydrolases