title: Detailed mutational analysis of Vga(A) interdomain linker: implication for antibiotic resistance specificity and mechanism.
abstract: Detailed mutational analysis examines the roles of individual residues of the Vga(A) linker in determining the antibiotic resistance phenotype. It defines a narrowed region of residues 212 to 220 whose composition determines the resistance specificity to lincosamides, pleuromutilins, and/or streptogramins A. From the analogy with the recently described function of the homologous ABC-F protein EttA as a translational factor, we infer that the Vga(A) linker interacts with the ribosome and directly or indirectly affects the binding of the respective antibiotic.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Lenart
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vimberg
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Ludmila
authors.initials: L
authors.lastname: Vesela
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Janata
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic gnovotna@biomed.cas.cz.
authors.forename: Gabriela
authors.identifier: http://orcid.org/0000-0002-5464-4763
authors.initials: G
authors.lastname: Balikova Novotna
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D020285:Cryoelectron Microscopy; D004224:Diterpenes / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D055231:Lincosamides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011083:Polycyclic Compounds; D012270:Ribosomes / Q000378:metabolism; D025361:Streptogramins / Q000494:pharmacology; D000097567:Pleuromutilins
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004224:Diterpenes; D055231:Lincosamides; D011083:Polycyclic Compounds; D025361:Streptogramins; D000097567:Pleuromutilins