title: Transcriptomic comparison of thiamethoxam-resistance adaptation in resistant and susceptible strains of Aphis gossypii Glover.
abstract: A thiamethoxam-resistant strain of cotton aphid (ThR) strain displayed a 19.35-fold greater resistance to thiamethoxam compared to a susceptible cotton aphid (SS) strain. Solexa sequencing technology was used to investigate differentially expressed genes (DEGs) in cotton aphids in the context of thiamethoxam resistance. A total of 22,569,311 and 21,317,732 clean reads were obtained from the ThR and SS transcriptomes, respectively, and assembled into 35,222 non-redundant (Nr) consensus sequences. The expression of 620 unigenes changed significantly in the ThR libraries compared to the SS strain; 349 genes were up-regulated, and 271 genes were down-regulated (P≤0.001). Expression levels of ribosomal proteins, ATP synthase, cytochrome c oxidase, ecdysteroid UDP-glucosyltransferase and esterase were up-regulated significantly in the ThR strain compared to the SS strain. The genes of cuticle proteins, salivary proteins, and fibroin heavy chain decreased dramatically. One nicotinic acetylcholine receptor (nAChR) α subunit was down-regulated in the ThR strain. The expression levels of 10 differentially expressed unigenes were confirmed using real-time RT-PCR, and the observed trends in gene expression matched the Solexa expression profiles. Specific single-nucleotide polymorphisms (SNPs) in nAChRs that cause amino acid substitution were found from the ThR and SS stains respectively. These data illustrate that genetic changes in nAChR genes and up-regulated ribosomal proteins, ecdysteroid UDP-glucosyltransferase, cytochrome c oxidase, esterase and peroxidase may confer the tolerance of resistant cotton aphids to thiamethoxam.
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Yiou
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Tianfei
authors.initials: T
authors.lastname: Peng
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xi
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Xin
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Bi
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China. Electronic address: shangqingli@163.com.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000818:Animals; D001042:Aphids / Q000235:genetics / Q000502:physiology*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000494:pharmacology*; D010078:Oxazines / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D000077922:Thiamethoxam; D013844:Thiazoles / Q000494:pharmacology*; D059467:Transcriptome
chemical_list: D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013844:Thiazoles; D000077922:Thiamethoxam
keywords: Aphis gossypii; Nicotinic acetylcholine receptor; Resistance; Ribosomal protein; Thiamethoxam