title: Cell-penetrating and neurotargeting dendritic siRNA nanostructures.
abstract: We report the development of dendritic siRNA nanostructures that are able to penetrate even difficult to transfect cells such as neurons with the help of a special receptor ligand. The nanoparticles elicit strong siRNA responses, despite the dendritic structure. An siRNA dendrimer directed against the crucial rabies virus (RABV) nucleoprotein (N protein) and phosphoprotein (P protein) allowed the suppression of the virus titer in neurons below the detection limit. The cell-penetrating siRNA dendrimers, which were assembled using click chemistry, open up new avenues toward finding novel molecules able to cure this deadly disease.
authors.affiliation: Center for Integrative Protein Science, Department of Chemistry, Ludwig-Maximilians University München, Butenandtstrasse 5-13, 81377 Munich (Germany) http://www.carellgroup.de.
authors.forename: Korbinian
authors.initials: K
authors.lastname: Brunner
authors.forename: Johannes
authors.initials: J
authors.lastname: Harder
authors.forename: Tobias
authors.initials: T
authors.lastname: Halbach
authors.forename: Julian
authors.initials: J
authors.lastname: Willibald
authors.forename: Fabio
authors.initials: F
authors.lastname: Spada
authors.forename: Felix
authors.initials: F
authors.lastname: Gnerlich
authors.forename: Konstantin
authors.initials: K
authors.lastname: Sparrer
authors.forename: Andreas
authors.initials: A
authors.lastname: Beil
authors.forename: Leonhard
authors.initials: L
authors.lastname: Möckl
authors.forename: Christoph
authors.initials: C
authors.lastname: Bräuchle
authors.forename: Karl-Klaus
authors.initials: KK
authors.lastname: Conzelmann
authors.forename: Thomas
authors.initials: T
authors.lastname: Carell
mesh_terms: D002851:Chromatography, High Pressure Liquid; D050091:Dendrimers; D049329:Nanostructures; D034741:RNA, Small Interfering / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D050091:Dendrimers; D034741:RNA, Small Interfering
keywords: anandamide; antiviral agents; drug delivery; gene silencing; stem cells