title: Neuroprotection by scorpion venom heat resistant peptide in 6-hydroxydopamine rat model of early-stage Parkinson's disease.
abstract: Neuroprotective effect of scorpion venom on Parkinson's disease (PD) has already been reported. The present study was aimed to investigate whether scorpion venom heat resistant peptide (SVHRP) could attenuate ultrastructural abnormalities in mitochondria and oxidative stress in midbrain neurons of early-stage PD model. The early-stage PD model was established by injecting 6-hydroxydopamine (6-OHDA) (20 μg/3 μL normal saline with 0.1% ascorbic acid) into the striatum of Sprague Dawley (SD) rats unilaterally. The rats were intraperitoneally administered with SVHRP (0.05 mg/kg per day) or vehicle (saline) for 1 week. Two weeks after 6-OHDA treatment, the rats received behavior tests for validation of model. Three weeks after 6-OHDA injection, the immunoreactivity of dopaminergic neurons were detected by immunohistochemistry staining, and the ultrastructure of neuronal mitochondria in midbrain was observed by electron microscope. In the meantime, the activities of monoamine oxidase-B (MAO-B), superoxide dismutase (SOD) and content of malondialdehyde (MDA) in the mitochondria of the midbrain neurons, as well as the inhibitory ability of hydroxyl free radical and the antioxidant ability in the serum, were measured by corresponding kits. The results showed that 6-OHDA reduced the optical density of dopaminergic neurons, induced damage of mitochondrial ultrastructure of midbrain neurons, decreased SOD activity, increased MAO-B activity and MDA content, and reduced the antioxidant ability of the serum. SVHRP significantly reversed the previous harmful effects of 6-OHDA in early-stage PD model. These findings indicate that SVHRP may contribute to neuroprotection by preventing biochemical and ultrastructure damage changes which occur during early-stage PD.
authors.affiliation: Department of Physiology; Laboratory of Function, Dalian Medical University, Dalian 116044, China. dlshengming@163.com.
authors.forename: Sheng-Ming
authors.initials: SM
authors.lastname: Yin
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Yu
authors.forename: Sheng-Long
authors.initials: SL
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: An
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Dong-Mei
authors.initials: DM
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Wan-Qin
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003342:Corpus Striatum; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008636:Mesencephalon / Q000166:cytology; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D016627:Oxidopamine; D010300:Parkinson Disease / Q000188:drug therapy*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012604:Scorpion Venoms / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018696:Neuroprotective Agents; D010455:Peptides; D012604:Scorpion Venoms; D008315:Malondialdehyde; D016627:Oxidopamine; D013482:Superoxide Dismutase