title: Modulation properties of factors released by bone marrow stromal cells on activated microglia: an in vitro study.
abstract: In the present paper we develop a new non-cell based (cell-free) therapeutic approach applied to BV2 microglial cells and spinal cord derived primary microglia (PM) using conditioned media from rat bone marrow stromal cells (BMSCs-CM). First we collected conditioned media (CM) from either naive or injured rat spinal cord tissue (SCI-CM, inflammatory stimulation agent) and from rat bone marrow stromal cells (BMSCs-CM, therapeutic immunomodulation agent). They were both subsequently checked for the presence of chemokines and growth, neurotrophic and neural migration factors using proteomics analysis. The data clearly showed that rat BMSCs-CM contain in vitro growth factors, neural migration factors, osteogenic factors, differentiating factors and immunomodulators, whereas SCI-CM contain chemokines, chemoattractant factors and neurotrophic factors. Afterwards we determined whether the BMSCs-CM affect chemotactic activity, NO production, morphological and pro-apoptotic changes of either BV2 or PM cells once activated with SCI-CM. Our results confirm the anti-migratory and NO-inhibitory effects of BMSCs-CM on SCI-CM-activated microglia with higher impact on primary microglia. The cytotoxic effect of BMSCs-CM occurred only on SCI-CM-stimulated BV2 cells and PM, not on naive BV2 cells, nor on PM. Taken together, the molecular cocktail found in BMSCs-CM is favorable for immunomodulatory properties.
authors.affiliation: 1] Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France [2] Institute of Neurobiology, Slovak Academy of Sciences, Center of Excellence for Brain Research, Soltesovej 4-6, 040 01 Kosice, Slovakia.
authors.forename: Dasa
authors.initials: D
authors.lastname: Cizkova
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Devaux
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Le Marrec-Croq
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Franck
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Center of Excellence for Brain Research, Soltesovej 4-6, 040 01 Kosice, Slovakia.
authors.forename: Lucia
authors.initials: L
authors.lastname: Slovinska
authors.affiliation: Institute of Neurobiology, Slovak Academy of Sciences, Center of Excellence for Brain Research, Soltesovej 4-6, 040 01 Kosice, Slovakia.
authors.forename: Juraj
authors.initials: J
authors.lastname: Blasko
authors.affiliation: Associated Tissue Bank of the Pavol Jozef Safarik University, Faculty of Medicine, University Hospital of. Louis Pasteur in Kosice, Trieda SNP 1, 040 66 Kosice, Slovakia.
authors.forename: Jan
authors.initials: J
authors.lastname: Rosocha
authors.affiliation: Associated Tissue Bank of the Pavol Jozef Safarik University, Faculty of Medicine, University Hospital of. Louis Pasteur in Kosice, Trieda SNP 1, 040 66 Kosice, Slovakia.
authors.forename: Timea
authors.initials: T
authors.lastname: Spakova
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Lefebvre
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fournier
authors.affiliation: Laboratoire PRISM: Protéomique, Réponse Inflammatoire, Spectrométrie de Masse, -U1192 INSERM, Bât SN3, 1er étage, Université de Lille 1, F-59655 Villeneuve d'Ascq, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Salzet
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D007155:Immunologic Factors / Q000378:metabolism / Q000494:pharmacology; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D017628:Microglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D007155:Immunologic Factors; D036341:Intercellular Signaling Peptides and Proteins