title: A P4-ATPase gene GbPATP of cotton confers chilling tolerance in plants.
abstract: Members of the P4 subfamily of P-type ATPases are implicated in generating lipid asymmetry between the two lipid leaflets of the plasma membrane in Arabidopsis and are important for resistance to low temperatures, but the function of P4-ATPases in cotton remains unclear. In this study, we found using quantitative reverse transcription-PCR analysis that the expression of the P4-ATPase gene GbPATP in cotton was induced at low temperatures. In addition, GbPATP-silenced cotton plants were more sensitive to low temperatures and exhibited greater malondialdehyde (MDA) content and lower catalase (CAT) activity than the control plants. GbPATP transgenic tobacco plants showed better chilling tolerance, had a lower MDA content and had higher CAT activity than wild-type plants under low-temperature treatment. The green fluorescent protein (GFP)-GbPATP fusion protein was found to be localized to the cell plasma membrane. Collectively, the results suggest that GbPATP functions as a P4-ATPase and plays an important role in improving chilling tolerance in plant.
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China These authors contributed equally to this work.
authors.forename: Tingli
authors.initials: T
authors.lastname: Liu
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China These authors contributed equally to this work.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Guo
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Lian
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Tianzi
authors.initials: T
authors.lastname: Chen
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Xitie
authors.initials: X
authors.lastname: Ling
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China.
authors.forename: Aiming
authors.initials: A
authors.lastname: Liu
authors.affiliation: School of life sciences, Anhui agricultural University, Hefei, Anhui 230036, China.
authors.forename: Rongfu
authors.initials: R
authors.lastname: Wang
authors.affiliation: Provincial key laboratory of agrobiology, Jiangsu Academy of Agricultural Sciences, Jiangsu, Nanjing 210014, China zhbl2248@hotmail.com.
authors.forename: Baolong
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003080:Cold Temperature; D019295:Computational Biology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport; D013312:Stress, Physiological / Q000235:genetics; D014026:Nicotiana / Q000235:genetics / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D010940:Plant Proteins; D000251:Adenosine Triphosphatases
keywords: Chilling tolerance; GbPATP; Malondialdehyde content; P4-ATPases; Transgenic tobacco; Virus-induced gene silencing