title: Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica.
abstract: Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greater stability. The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol% 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. A quartz crystal microbalance with dissipation monitoring was used to monitor the process of vesicle adsorption and tBLM self-assembly, and atomic force microscopy was performed to characterize the structural properties of the tBLM obtained. Whereas tether-containing lipid vesicles ruptured neither spontaneously nor as a result of osmotic shock, introduction of an amphipathic α-helical (AH) peptide induced vesicle rupture and subsequent tBLM formation. Taken together, our findings suggest that the AH peptide is an efficient means of rupturing vesicles of both simple and complex composition, and is, therefore, useful for formation of tBLMs on solid and mesoporous materials for applications in biotechnology.
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, 412 96, Göteborg, Sweden, maria.wallin@chalmers.se.
authors.forename: Maria
authors.initials: M
authors.lastname: Wallin
authors.forename: Jae-Hyeok
authors.initials: JH
authors.lastname: Choi
authors.forename: Seong Oh
authors.initials: SO
authors.lastname: Kim
authors.forename: Nam-Joon
authors.initials: NJ
authors.lastname: Cho
authors.forename: Martin
authors.initials: M
authors.lastname: Andersson
mesh_terms: D000327:Adsorption; D008051:Lipid Bilayers / Q000737:chemistry*; D009997:Osmotic Pressure; D010455:Peptides / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D008051:Lipid Bilayers; D010455:Peptides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D053835:Unilamellar Liposomes; D011092:Polyethylene Glycols; D012822:Silicon Dioxide; C028694:1-palmitoyl-2-oleoylphosphatidylcholine