title: Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors.
abstract: Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using a special waveguide based on a silver halide fiber was used for probing the heat-induced secondary structure and conformation changes of bovine serum albumin (BSA). From the secondary derivative and the curve fitting of the obtained ATR-FTIR spectra, the changes of the BSA secondary structure with temperature were clearly identified. Two different thermal denaturation temperature ranges (i.e., 50-52 and 80-82 °C, at which a change of the protein structure occurred) were determined, while only one denaturation temperature was previously identified via classical FTIR measurements. Additionally, taking advantage of two-dimensional correlation spectroscopy more detailed information on changes of the protein secondary structure was revealed. The developed method facilitates in situ, sensitive, and more in-depth probing of protein secondary structures, which represents a significant advancement compared to conventional characterization methods.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Hefei 230026, P.R. China. hqyu@ustc.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Lu
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Abraham
authors.initials: A
authors.lastname: Katzir
authors.forename: Yosef
authors.initials: Y
authors.lastname: Raichlin
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
authors.forename: Boris
authors.initials: B
authors.lastname: Mizaikoff
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D005336:Fiber Optic Technology / Q000379:methods*; D006358:Hot Temperature; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D012710:Serum Albumin, Bovine