title: [ABCG1 transporter gene expression in peripheral blood mononuclear cells of patients with atherosclerosis].
abstract: Accumulation of cholesterol in arterial wall macrophages is a main hallmark of atherosclerosis. The ABCG1 transporter mediates cholesterol efflux to high density lipoproteins (HDL) and plays an important role in macrophage foam cell formation. The goal of our study was to investigate the potential role of ABCG1 in atherosclerosis development in humans. ABCG1 gene expression has been examined in leukocytes, monocytes and monocyte-derived macrophages of patients with atherosclerosis and in the control group. Real time PCR and Western blotting were used to determine ABCG1 mRNA and ABCG1 protein levels. Monocyte ABCG1 mRNA level was inversely correlated with the rate of artery occlusion (r = -0.45, P = 0.016). Patients with 100% artery occlusions had decreased monocyte ABCG1 mRNA levels compared to patients who had smaller plaques and controls (P < 0.05). ABCG1 mRNA (P < 0.001) and ABCG1 protein (P < 0.05) levels in macrophages of patients with coronary artery stenosis were significantly reduced compared to the control group. No significant correlation between the ABCG1 gene expression in mononuclear cells and HDL cholesterol concentration has been found. Our study suggests that decrease in the ABCG1 gene expression in macrophages is associated with atherosclerosis.
authors.forename: V V
authors.initials: VV
authors.lastname: Miroshnikova
authors.forename: E P
authors.initials: EP
authors.lastname: Demina
authors.forename: N V
authors.initials: NV
authors.lastname: Maĭorov
authors.forename: V V
authors.initials: VV
authors.lastname: Davydenko
authors.forename: P S
authors.initials: PS
authors.lastname: Kur'ianov
authors.forename: V N
authors.initials: VN
authors.lastname: Vavilov
authors.forename: A G
authors.initials: AG
authors.lastname: Vinogradov
authors.forename: A D
authors.initials: AD
authors.lastname: Denisenko
authors.forename: A L
authors.initials: AL
authors.lastname: Shvartsman
mesh_terms: D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D001158:Arteries / Q000378:metabolism / Q000473:pathology; D050197:Atherosclerosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D001692:Biological Transport; D008076:Cholesterol, HDL / Q000378:metabolism; D054059:Coronary Occlusion / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: C419353:ABCG1 protein, human; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D008076:Cholesterol, HDL; D012333:RNA, Messenger