title: The galactolipase activity of Fusarium solani (phospho)lipase.
abstract: The purified (phospho)lipase of Fusarium solani (FSL), was known to be active on both triglycerides and phospholipids. This study aimed at assessing the potential of this enzyme in hydrolyzing galactolipids. FSL was found to hydrolyze at high rates of synthetic medium chains monogalactosyldiacylglycerol (4658±146U/mg on DiC8-MGDG) and digalactosyldiacylglycerol (3785±83U/mg on DiC8-DGDG) and natural long chain monogalactosyldiacylglycerol extracted from leek leaves (991±85U/mg). It is the microbial enzyme with the highest activity on galactolipids identified so far with a level of activity comparable to that of pancreatic lipase-related protein 2. FSL maximum activity on galactolipids was measured at pH8. The analysis of the hydrolysis product of natural MGDG from leek showed that FSL hydrolyzes preferentially the ester bond at the sn-1 position of galactolipids. To investigate the structure-activity relationships of FSL, a 3D model of this enzyme was built. In silico docking of medium chains MGDG and DGDG and phospholipid in the active site of FSL reveals structural solutions which are in concordance with in vitro tests.
authors.affiliation: Univertsity of Sfax, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038 Sfax, Tunisia.
authors.forename: Raida
authors.initials: R
authors.lastname: Jallouli
authors.affiliation: Université Tunis-ElManar, Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Tunis 1002, Tunisia.
authors.forename: Houcemeddine
authors.initials: H
authors.lastname: Othman
authors.affiliation: CNRS, Aix-Marseille Université, UMR 7282 Enzymologie Interfaciale et Physiologie de la Lipolyse, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Sawsan
authors.initials: S
authors.lastname: Amara
authors.affiliation: CNRS, Aix-Marseille Université, UMR 7282 Enzymologie Interfaciale et Physiologie de la Lipolyse, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Goetz
authors.initials: G
authors.lastname: Parsiegla
authors.affiliation: CNRS, Aix-Marseille Université, UMR 7282 Enzymologie Interfaciale et Physiologie de la Lipolyse, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Carriere
authors.affiliation: Université Tunis-ElManar, Institut Pasteur de Tunis, Laboratoire des Venins et Biomolécules Thérapeutiques LR11IPT08, Tunis 1002, Tunisia.
authors.forename: Najet
authors.initials: N
authors.lastname: Srairi-Abid
authors.affiliation: Univertsity of Sfax, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038 Sfax, Tunisia.
authors.forename: Youssef
authors.initials: Y
authors.lastname: Gargouri
authors.affiliation: Univertsity of Sfax, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038 Sfax, Tunisia. Electronic address: sofiane_bezzine@yahoo.com.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bezzine
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D057075:Enzyme Assays; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry* / Q000201:enzymology; D038983:Galactolipids / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008049:Lipase / Q000737:chemistry; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D019697:Onions / Q000737:chemistry; D010740:Phospholipases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D005656:Fungal Proteins; D038983:Galactolipids; D011994:Recombinant Proteins; C007388:digalactosyldiacylglycerol; C009909:monogalactosyldiacylglycerol; D010740:Phospholipases; D002265:Carboxylic Ester Hydrolases; C019262:galactolipase; D008049:Lipase; C075988:pancreatic lipase related protein 2
keywords: Fusarium solani (phospho)lipase; Galactolipase; Galactolipid; In silico docking; Leek