title: A new model to study sleep deprivation-induced seizure.
abstract: OBJECTIVE
  A relationship between sleep and seizures is well-described in both humans and rodent animal models; however, the mechanism underlying this relationship is unknown. Using Drosophila melanogaster mutants with seizure phenotypes, we demonstrate that seizure activity can be modified by sleep deprivation.
  
  
  METHODS
  Seizure activity was evaluated in an adult bang-sensitive seizure mutant, stress sensitive B (sesB(9ed4)), and in an adult temperature sensitive seizure mutant seizure (sei(ts1)) under baseline and following 12 h of sleep deprivation. The long-term effect of sleep deprivation on young, immature sesB(9ed4) flies was also assessed.
  
  
  METHODS
  Laboratory.
  
  
  METHODS
  Drosophila melanogaster.
  
  
  METHODS
  Sleep deprivation.
  
  
  RESULTS
  Sleep deprivation increased seizure susceptibility in adult sesB(9ed4)/+ and sei(ts1) mutant flies. Sleep deprivation also increased seizure susceptibility when sesB was disrupted using RNAi. The effect of sleep deprivation on seizure activity was reduced when sesB(9ed4)/+ flies were given the anti-seizure drug, valproic acid. In contrast to adult flies, sleep deprivation during early fly development resulted in chronic seizure susceptibility when sesB(9ed4)/+ became adults.
  
  
  CONCLUSIONS
  These findings show that Drosophila is a model organism for investigating the relationship between sleep and seizure activity.
authors.affiliation: Department of Neurology, Washington University School of Medicine, Saint Louis, MO.
authors.forename: Brendan P
authors.initials: BP
authors.lastname: Lucey
authors.affiliation: Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO.
authors.forename: Averi
authors.initials: A
authors.lastname: Leahy
authors.affiliation: Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO.
authors.forename: Regine
authors.initials: R
authors.lastname: Rosas
authors.affiliation: Department of Anatomy and Neurobiology, Washington University School of Medicine, Saint Louis, MO.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Shaw
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D005260:Female; D008297:Male; D000226:Mitochondrial ADP, ATP Translocases / Q000235:genetics / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D012640:Seizures / Q000188:drug therapy / Q000209:etiology* / Q000235:genetics / Q000503:physiopathology*; D012890:Sleep / Q000235:genetics; D012892:Sleep Deprivation / Q000150:complications* / Q000235:genetics / Q000503:physiopathology*; D013696:Temperature; D013997:Time Factors; D014635:Valproic Acid / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D000927:Anticonvulsants; D029721:Drosophila Proteins; C491544:sesB protein, Drosophila; D014635:Valproic Acid; D000226:Mitochondrial ADP, ATP Translocases
keywords: Drosophila melanogaster; seizure; sleep homeostasis