title: Short and long-term exposure to diclofenac alter oxidative stress status in common carp Cyprinus carpio.
abstract: Diclofenac (DCF) has been detected in significant amounts in municipal treated wastewater effluent. Diverse studies report that trace concentrations of DCF may induce toxic effects on different aquatic organisms as well as developmental, reproductive and renal damage. This study aimed to determine whether short and long-term exposure to DCF alter the oxidative stress (OS) status in blood, muscle, gills, brain and liver of common carp Cyprinus carpio. The median lethal concentration of DCF at 96 h (96-h LC50) and subsequently the lowest observed adverse effect level were determined. Carp were exposed (short and long-term) to the latter value for different exposure times (4 and 24 days) and the following biomarkers were evaluated in gill, brain, liver and blood: hydroperoxides content (HPC), lipid peroxidation (LPX), protein carbonyl content (PCC) and the activity of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Also, the DCF was determined by LC-MS/MS. Significant increases in HPC, LPX and PCC were observed respect to control (P < 0.05) particularly in blood, muscle, gill, brain and liver. SOD, CAT and GPx activity also increased in these organs, with respect to controls (P < 0.05). DCF concentrations decreased and increased in water system and carp, respectively. Cyprinus carpio exposed to DCF was affected in OS status during the initial days of the study (at 4 days), exhibiting an increased response at 24 days in blood and liver. In contrast, a decrease was observed in muscle, gills and brain at 24 days with respect to 4 days. In conclusion, DCF induces OS on blood, muscle, gills, brain and liver in the carp C. carpio in short and long-term exposure. The biomarkers employed in this study are useful in the assessment of the environmental impact of this agent on aquatic species.
authors.affiliation: Laboratorio de Toxicología Ambiental, Facultad de Química, Universidad Autónoma del Estado de México, Paseo Colón intersección Paseo Tollocan s/n. Col. Residencial Colón, 50120, Toluca, Estado de México, Mexico.
authors.forename: Karinne
authors.initials: K
authors.lastname: Saucedo-Vence
authors.forename: Octavio
authors.initials: O
authors.lastname: Dublán-García
authors.forename: Leticia Xochitl
authors.initials: LX
authors.lastname: López-Martínez
authors.forename: Gabriela
authors.initials: G
authors.lastname: Morachis-Valdes
authors.forename: Marcela
authors.initials: M
authors.lastname: Galar-Martínez
authors.forename: Hariz
authors.initials: H
authors.lastname: Islas-Flores
authors.forename: Leobardo Manuel
authors.initials: LM
authors.lastname: Gómez-Oliván
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002347:Carps / Q000378:metabolism*; D004008:Diclofenac / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D014874:Water Pollutants, Chemical; D004008:Diclofenac