title: Mitochondrial calcium handling in normotensive and spontaneously hypertensive rats: correlation with systolic blood pressure levels.
abstract: The aim was to study the mitochondrial Ca(2+) handling of mitochondria isolated from normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) hearts and to establish a possible correlation with systolic blood pressure (SBP). Mitochondrial swelling after Ca(2+) addition, Ca(2+)-retention capacity (CRC) by calcium green method, and membrane potential (ΔΨm) were assessed. SBP was 124±1 (WKY) and 235±6mmHg (SHR). CRC, Ca(2+) response and ΔΨm were lower in SHR than WKY mitochondria. The conclusion is: the more depolarized state of SHR than WKY mitochondria results in an abnormal Ca(2+) handling and this event is closely associated with the SBP.
authors.affiliation: Centro de Investigaciones Cardiovasculares, CCT-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Alejandro Ciocci
authors.initials: AC
authors.lastname: Pardo
authors.affiliation: Centro de Investigaciones Cardiovasculares, CCT-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina.
authors.forename: Gustavo J
authors.initials: GJ
authors.lastname: Rinaldi
authors.affiliation: Centro de Investigaciones Cardiovasculares, CCT-CONICET, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina. Electronic address: smosca@med.unlp.edu.ar.
authors.forename: Susana M
authors.initials: SM
authors.lastname: Mosca
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002118:Calcium / Q000378:metabolism*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D002118:Calcium
keywords: Ca(2+) response; Ca(2+)-retention capacity; SHR; WKY; mPTP; ΔΨm