title: Loss of lamin A but not lamin C expression in epithelial ovarian cancer cells is associated with metastasis and poor prognosis.
abstract: OBJECTIVE
  To elucidate the different roles of lamin A and lamin C in the metastasis of epithelial ovarian cancer (EOC) by examining their expression in EOC tissues and cell lines and their correlations with clinicopathological features.
  
  
  METHODS
  The expression of lamin A and lamin C was assessed in ovarian tissues from 61 EOC patients and 13 normal, 14 benign controls, respectively, using immunohistochemistry. Two pairs of EOC cell lines: HO-8910, HO-8910PM, SKOV-3 and SKOV-3ip were also examined to see the differential expression patterns of lamin A and lamin C.
  
  
  RESULTS
  Lamin A expression was significantly lower in EOC tissues than that in normal and benign ovarian tissues (p<0.05), while lamin C expression was not. Lamin A expression level was closely associated with pathological grades, clinical stages, peritoneal metastasis and lymph node metastasis (all p<0.05). The progression-free survival rate of the EOC patients with low lamin A expression level was remarkably lower than that of the EOC patients with high expression level (p<0.05). Lamin A detected by PCR, Western Blot and immunocytochemistry also showed a significantly lower expression level in HO-8910PM than that in HO-8910 (p<0.05), but not for lamin C. There was no difference between SKOV-3 and SKOV-3ip cells.
  
  
  CONCLUSIONS
  The results suggested that loss of lamin A but not lamin C expression in EOC was related to metastasis and poor prognosis. Lamin A may play a critical role in the metastasis of EOC.
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Gong
authors.affiliation: Department of Gynecology and Obstetrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, PR China.
authors.forename: Puxiang
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, PR China. Electronic address: xyw2007@csu.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D000077216:Carcinoma, Ovarian Epithelial; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D034904:Lamin Type A / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000556:secondary*; D011379:Prognosis; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D034904:Lamin Type A; C440308:lamin C
keywords: Epithelial ovarian cancer; Lamin A; Lamin C; Metastasis; Prognosis