title: Age-dependent association of MDM2 promoter polymorphisms and uterine leiomyoma in South-East Iran: A preliminary report.
abstract: OBJECTIVE
  Murine double minute clone 2 (MDM2) is an important regulator of p53 tumor suppressor protein. Because increased MDM2 expression has been observed in different tumors, its polymorphisms are proposed to be associated with accelerated tumor formation. The aim of this study was to examine the association between T309G (rs2279744) and 40-bp Insertion/deletion (rs3730485) polymorphisms of the MDM2 gene and risk of uterine leiomyoma (UL).
  
  
  METHODS
  We analyzed the MDM2 gene polymorphisms of 154 UL patients and 197 healthy controls by polymerase chain reaction (PCR) or PCR restriction fragment length polymorphism (RFLP) methods.
  
  
  RESULTS
  The frequency of MDM2 T309G polymorphism genotypes was not different between UL women and controls. Although there was an association between MDM2 40-bp del/del genotype and UL before and after adjustment for age, the association between Insertion/deletion (Indel) genotype and UL was significant after adjustment for age.
  
  
  CONCLUSIONS
  MDM2 T309G polymorphism was not associated with UL and MDM2 40-bp Indel polymorphism could be an age-related risk factor for UL.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.forename: Azam
authors.initials: A
authors.lastname: Hajizadeh
authors.forename: Maryam
authors.initials: M
authors.lastname: Khodamian
authors.forename: Atefeh
authors.initials: A
authors.lastname: Pejman
authors.forename: Kimia
authors.initials: K
authors.lastname: Fazeli
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaei
mesh_terms: D000328:Adult; D000367:Age Factors; D000483:Alleles; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007492:Iran; D007889:Leiomyoma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D014594:Uterine Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
keywords: MDM2; gene; polymorphism; uterine leiomyoma