title: High-throughput epitope discovery reveals frequent recognition of neo-antigens by CD4+ T cells in human melanoma.
abstract: Tumor-specific neo-antigens that arise as a consequence of mutations are thought to be important for the therapeutic efficacy of cancer immunotherapies. Accumulating evidence suggests that neo-antigens may be commonly recognized by intratumoral CD8+ T cells, but it is unclear whether neo-antigen-specific CD4+ T cells also frequently reside within human tumors. In view of the accepted role of tumor-specific CD4+ T-cell responses in tumor control, we addressed whether neo-antigen-specific CD4+ T-cell reactivity is a common property in human melanoma.
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Carsten
authors.initials: C
authors.lastname: Linnemann
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Marit M
authors.initials: MM
authors.lastname: van Buuren
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Laura
authors.initials: L
authors.lastname: Bies
authors.affiliation: Department of Clinical Oncology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Els M E
authors.initials: EM
authors.lastname: Verdegaal
authors.affiliation: AIMM Therapeutics B.V., Amsterdam, the Netherlands.
authors.forename: Remko
authors.initials: R
authors.lastname: Schotte
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Jorg J A
authors.initials: JJ
authors.lastname: Calis
authors.affiliation: Cancer Genome Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom.
authors.forename: Sam
authors.initials: S
authors.lastname: Behjati
authors.affiliation: Central Genomics Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Arno
authors.initials: A
authors.lastname: Velds
authors.affiliation: Peptide Synthesis Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Henk
authors.initials: H
authors.lastname: Hilkmann
authors.affiliation: Peptide Synthesis Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Dris El
authors.initials: DE
authors.lastname: Atmioui
authors.affiliation: Department of Clinical Oncology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Marten
authors.initials: M
authors.lastname: Visser
authors.affiliation: Cancer Genome Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, United Kingdom.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Stratton
authors.affiliation: 1] Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands. [2] Division of Medical Oncology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: John B A G
authors.initials: JB
authors.lastname: Haanen
authors.affiliation: AIMM Therapeutics B.V., Amsterdam, the Netherlands.
authors.forename: Hergen
authors.initials: H
authors.lastname: Spits
authors.affiliation: Department of Clinical Oncology, Leiden University Medical Center, Leiden, the Netherlands.
authors.forename: Sjoerd H
authors.initials: SH
authors.lastname: van der Burg
authors.affiliation: Division of Immunology, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
authors.forename: Ton N M
authors.initials: TN
authors.lastname: Schumacher
mesh_terms: D017951:Antigen Presentation / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007167:Immunotherapy; D008545:Melanoma / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D009154:Mutation; D051560:Proto-Oncogene Proteins c-bcl-6; D051020:bcl-X Protein / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; C494811:BCL2L1 protein, human; C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D018984:Epitopes, T-Lymphocyte; D051560:Proto-Oncogene Proteins c-bcl-6; D051020:bcl-X Protein