title: Compact halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes.
abstract: To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
authors.affiliation: Laboratory for Nano-Bio Probes, Quantitative Biology Center, Riken, Suita, 565-0874, Osaka, Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Komatsuzaki
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ohyanagi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Tsukasaki
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Miyanaga
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ueda
authors.forename: Takashi
authors.initials: T
authors.lastname: Jin
mesh_terms: D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D005456:Fluorescent Dyes / Q000737:chemistry; D006219:Halogens / Q000737:chemistry*; D008024:Ligands; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D058726:Nanoconjugates / Q000737:chemistry / Q000648:ultrastructure; D045663:Quantum Dots; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D006219:Halogens; D008024:Ligands; D058726:Nanoconjugates; D043562:Receptors, G-Protein-Coupled
keywords: fluorescence; halo tags; imaging; membranes; proteins; quantum dots; single-molecule studies