title: Prepubertal exposure to genistein alleviates di-(2-ethylhexyl) phthalate induced testicular oxidative stress in adult rats.
abstract: Di-(2-ethylhexyl) phthalate (DEHP) is the most widely used plastizer in the world and can suppress testosterone production via activation of oxidative stress. Genistein (GEN) is one of the isoflavones ingredients exhibiting weak estrogenic and potentially antioxidative effects. However, study on reproductive effects following prepubertal multiple endocrine disrupters exposure has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from postnatal day 22 (PND22) to PND35 with vehicle control, GEN at 50 mg/kg body weight (bw)/day (G), DEHP at 50, 150, 450 mg/kg bw/day (D50, D150, D450) and their mixture (G + D50, G + D150, G + D450). On PND90, general morphometry (body weight, AGD, organ weight, and organ coefficient), testicular redox state, and testicular histology were studied. Our results indicated that DEHP could significantly decrease sex organs weight, organ coefficient, and testicular antioxidative ability, which largely depended on the dose of DEHP. However, coadministration of GEN could partially alleviate DEHP-induced reproductive injuries via enhancement of testicular antioxidative enzymes activities, which indicates that GEN has protective effects on DEHP-induced male reproductive system damage after prepubertal exposure and GEN may have promising future in its curative antioxidative role for reproductive disorders caused by other environmental endocrine disruptors.
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: Lian-Dong
authors.initials: LD
authors.lastname: Zhang
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: He-Cheng
authors.initials: HC
authors.lastname: Li
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: Tie
authors.initials: T
authors.lastname: Chong
authors.affiliation: Department of Nephrology, Xi'an No. 4 Hospital, Xi'an, Shaanxi 710004, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yin
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: De-Lai
authors.initials: DL
authors.lastname: Fu
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China ; Department of Urology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Department of Urology, The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, Shaanxi 710004, China.
authors.forename: Zi-Ming
authors.initials: ZM
authors.lastname: Wang
mesh_terms: D000818:Animals; D004051:Diethylhexyl Phthalate / Q000008:administration & dosage*; D005260:Female; D019833:Genistein / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D013737:Testis / Q000187:drug effects / Q000254:growth & development; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D013739:Testosterone; D004051:Diethylhexyl Phthalate; D019833:Genistein