title: Lactosucrose attenuates intestinal inflammation by promoting Th2 cytokine production and enhancing CD86 expression in colitic rats.
abstract: Some oligosaccharides have immunoregulatory and anti-inflammatory functions in the intestine. This study investigated the immunoregulatory effect of lactosucrose (LS) on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitic rats. Alkaline phosphatase activity was increased but myeloperoxidase activity was decreased in the LS-TNBS group, as compared with the TNBS group (colitis rats without receiving LS). LS supplementation stimulated IL-4 and IL-10 production, while up-regulating CD86 expression in dendritic cells. LS supplementation reduced the ratio of CD80/CD86 and the ratio of IFN-γ/IL-4 compared to the TNBS group. Moreover, IFN-γ was significantly correlated with CD80 (r = 0.764, p < 0.01), whereas IL-4 was significantly correlated with CD86 (r = 0.489, p < 0.05). These results indicated that LS attenuated colitis by promoting the production of Th2-type cytokines and rebalancing the ratio of Th1/Th2 and that enhanced IL-4 production is correlated with enhanced CD86 expression in the gut. Therefore, LS is a functional food for patients with inflammatory bowel disease.
authors.affiliation: a State Key Laboratory of Food Science and Technology , Nanchang University , Nanchang , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ruan
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiaoliu
authors.initials: X
authors.lastname: Huang
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Deng
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000276:immunology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D051940:B7-2 Antigen / Q000819:agonists / Q000235:genetics* / Q000276:immunology; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D007249:Inflammation / Q000517:prevention & control; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D016753:Interleukin-10 / Q000819:agonists / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000819:agonists / Q000235:genetics / Q000276:immunology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D009195:Peroxidase / Q000235:genetics / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012720:Severity of Illness Index; D058408:Th1-Th2 Balance / Q000187:drug effects*; D014302:Trinitrobenzenesulfonic Acid; D014312:Trisaccharides / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497966:Cd86 protein, rat; D014312:Trisaccharides; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; C081752:galactosucrose; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D000469:Alkaline Phosphatase
keywords: Th1 and Th2 balance; dendritic cell; inflammation bowel disease; lactosucrose