title: Stereotaxis of mandibular nerve initial point of trigeminal ganglion in rats.
abstract: OBJECTIVE
  To acquire parameters for stereotaxis of the mandibular nerve initial point of the trigemenial ganglion (TG) and to test the accuracy of the acquired parameters for microinjection into the mandibular nerve initial point of TG in adult rats.
  
  
  METHODS
  Sprague-Dawley rats (260-270 g) were mounted onto a stereotaxic frame. The bregma was set as an anchor point and the three-dimensional parameters between the mandibular nerve initial point of the bilateral TGs and the bregma were measured in 25 rats. The accuracy of these parameters was tested using microinjection of Evans blue dye into the mandibular nerve initial point of the bilateral TGs in 30 rats and the injection sites were evaluated by dissection.
  
  
  RESULTS
  The three-dimensional parameters of the mandibular nerve initial point of the bilateral TGs were 3.5 ± 0.1 mm posterior and 3.6 ± 0.2 mm lateral to the bregma, and 12.0 ± 0.2 mm inferior to the skull surface. Accuracy for the microinjection of Evans blue dye into the mandibular nerve initial point of the bilateral TGs was 86.7% (52/60).
  
  
  CONCLUSIONS
  The acquired parameters served well for stereotaxis and microinjection of reagents into the mandibular nerve initial point of TG.
authors.forename: Xiu Feng
authors.initials: XF
authors.lastname: Tang
authors.forename: Rui Yun
authors.initials: RY
authors.lastname: Bi
authors.forename: Zhen
authors.initials: Z
authors.lastname: Meng
authors.forename: Ye Hua
authors.initials: YH
authors.lastname: Gan
mesh_terms: D059925:Anatomic Landmarks / Q000033:anatomy & histology; D000818:Animals; D002404:Catheterization / Q000295:instrumentation; D002508:Cephalometry / Q000379:methods; D004396:Coloring Agents; D003393:Cranial Sutures / Q000033:anatomy & histology; D005070:Evans Blue; D005624:Frontal Bone / Q000033:anatomy & histology; D021621:Imaging, Three-Dimensional / Q000379:methods; D008297:Male; D008340:Mandibular Nerve / Q000033:anatomy & histology*; D008845:Microinjections / Q000295:instrumentation / Q000379:methods; D009434:Neural Pathways / Q000033:anatomy & histology; D017288:Pain Threshold / Q000502:physiology; D010294:Parietal Bone / Q000033:anatomy & histology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012684:Sensory Thresholds / Q000502:physiology; D013238:Stereotaxic Techniques; D013594:Syringes; D014110:Touch / Q000502:physiology; D012668:Trigeminal Ganglion / Q000033:anatomy & histology*
chemical_list: D004396:Coloring Agents; D005070:Evans Blue