title: Using the quantum cell expansion system for the automated expansion of clinical-grade bone marrow-derived human mesenchymal stromal cells.
abstract: Bone marrow-derived human mesenchymal stromal cells (hMSCs) constitute a promising therapeutic approach. However, the extremely low frequency of hMSCs in bone marrow makes the translation of these regulatory cells to clinical therapies difficult for large patient populations. Here, we describe a good manufacturing practices-compliant procedure for the expansion of hMSCs using the Quantum Cell Expansion System. This closed and automated system allows the large-scale expansion of hMSCs while maintaining their multipotency, immunophenotype, morphology, and karyotype.
authors.affiliation: Program for Cell Enhancement and Technologies for Immunotherapy, Center for Cancer and Immunology Research, Children's National Health System, Washington, DC, USA.
authors.forename: Gema
authors.initials: G
authors.lastname: Martin-Manso
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Hanley
mesh_terms: D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D015925:Cryopreservation / Q000379:methods / Q000592:standards; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism