title: Separation of two major chalcones from Angelica keiskei by high-speed counter-current chromatography.
abstract: Angelica keiskei (Shin-sun cho) is an edible higher plant with the beneficial preventive effects on cancer, hypertension, and coronary heart disease. Two bioactive chalcones of Shin-sun cho, xanthoangelol (1) and 4-hydroxyderricin (2), were separated simultaneously by using high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-EtOAc-MeOH-H2O (9:5:9:4). Only nonconsuming processes, solvent fractionations and Sephadex LH-20 column chromatography, were conducted as presteps. Xanthoangelol (1, 35.9 mg, 99.9 % purity at 254 and 365 nm) and 4-hydroxyderricin (2, 4.4 mg, 98.7 % purity at 254 nm and 98.8 % purity at 365 nm) were successfully purified from 70 mg of the processed extract from A. keiskei. The structures of two compounds were confirmed by (1)H- and (13)C-NMR analysis.
authors.affiliation: Graduate School of Pharmaceutical Sciences (Ewha Global Top 5 Program), College of Pharmacy, Ewha Womans University, Seoul, 120-750, Korea.
authors.forename: Yun-Seo
authors.initials: YS
authors.lastname: Kil
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Nam
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Seo
mesh_terms: D029969:Angelica; D047188:Chalcones / Q000737:chemistry / Q000302:isolation & purification*; D002845:Chromatography / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves; D018547:Plant Stems
chemical_list: D047188:Chalcones; D010936:Plant Extracts