title: Profiling of serum and urinary microRNAs in children with atopic dermatitis.
abstract: BACKGROUND
  Atopic dermatitis (AD) is the most prevalent chronic inflammatory skin disease in children characterized by dermatitis and pruritus. MicroRNAs (miRNAs) have been shown as great potential biomarkers for disease fingerprints to predict prognostics. We aimed to identify miRNA signature from serum and urine for the prognosis of AD patient by genome-wide miRNA profiling analysis.
  
  
  METHODS
  Serum and urine from 30 children with AD and 28 healthy children were collected and their genome-wide miRNA expression profiles were measured by TaqMan-based array and confirmed by quantitative real-time PCR. Inflammatory factors in serum were detected by Antibody Array System.
  
  
  RESULTS
  miR-203 and miR-483-5p were significantly up-regulated in serum of children with AD compared with healthy children. The level of miR-483-5p in serum was significantly associated with other atopic conditions, such as rhinitis and/or asthma. However, miR-203 was markedly decreased in urine of children with AD compared with healthy children. Down-regulated miR-203 in urine was significant associated with abnormal level of serum IgE in AD patients. 7 inflammatory factors in serum were altered in children with AD compared with healthy children. Up-regulated miR-203 in serum was significantly associated with increased sTNFRI and sTNFRII.
  
  
  CONCLUSIONS
  Up-regulated miR-483-5p in serum may be indicative of other atopic conditions in children with AD. Down-regulated miR-203 in urine may serve as a biomarker for the severity of inflammation in children with AD.
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Yani
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Ruiqun
authors.initials: R
authors.lastname: Qi
authors.affiliation: Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Di
authors.affiliation: Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huo
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Hongduo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Dermatology, No. 1 Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Gao
mesh_terms: D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood / Q000235:genetics* / Q000652:urine; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007223:Infant; D018836:Inflammation Mediators / Q000032:analysis; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000652:urine*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D015415:Biomarkers; D018836:Inflammation Mediators; D035683:MicroRNAs