title: Detection of peanut (Arachis hypogaea) allergen by Real-time PCR method with internal amplification control.
abstract: Specific primer sets were designed based on the DNA sequence of Ara h 1, one of the major peanut (Arachis hypogaea) allergens, and a competitive internal amplification control (IAC) was designed by compound primer technology. By choosing 314 copies/PCR as the IAC dosage, a Real-time PCR method with IAC was established for detecting peanut allergen Ara h 1 DNA. The method showed high specificity with a detection limit of 0.005% peanut. A series of commercial food products with/without peanut components were tested. Among these products, the peanut allergen Ara h 1 DNA could be detected in 12 products labelled containing peanut ingredients, in two without a declaration of peanut and one labelled that was produced in a facility that produced peanut-containing foods. This indicates that the method is highly sensitive for the detection of peanut ingredients in foods.
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Wen-Ju
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Cai
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Guan
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, PR China. Electronic address: chenqincc@staff.shu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000485:Allergens / Q000276:immunology*; D052179:Antigens, Plant / Q000276:immunology*; D010367:Arachis / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D008565:Membrane Proteins; D010940:Plant Proteins / Q000276:immunology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C500889:Ara h 1 protein, Arachis hypogaea; D006023:Glycoproteins; D008565:Membrane Proteins; D010940:Plant Proteins
keywords: Allergen; Ara h 1; Internal amplification control (IAC); Peanut (Arachis hypogaea); Real-time PCR