title: Potentiation of antigen-specific antibody production by peptides derived from Ag85B of Mycobacterium tuberculosis.
abstract: To generate high-titer monoclonal antibodies, strong immuno-stimulation must be used for eliciting an intense cellular immune response. Here, we report that antigen-specific antibody production was potentiated by Peptide-25 derived from Ag85B of Mycobacterium tuberculosis, and that the production of antigen-specific IgG1 in particular was markedly potentiated; specifically, this occurred because the use of Peptide-25 resulted in an increase in the number of antigen-specific antibody-producing cells. We studied the activation of T cells by the peptide by examining gene expression. The observed expression pattern of GATA-3 and T-bet suggests that the peptide modulates the Th1/Th2 balance during immunization. This potentiation, which was remarkably high in BALB/c mice, could be applied in the immunization performed for monoclonal antibody production in vivo and in vitro.
authors.affiliation: Bio-Peak Co., Ltd., 584-70 Shimonojo, Takasaki 370-0854, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Kato
authors.affiliation: Molecular Composite Medicine Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566, Japan. Electronic address: y.hanyu@aist.go.jp.
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Hanyu
mesh_terms: D000217:Acyltransferases / Q000276:immunology*; D000276:Adjuvants, Immunologic; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology*; D005260:Female; D050990:GATA3 Transcription Factor / Q000096:biosynthesis; D015870:Gene Expression; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D020825:T-Box Domain Proteins / Q000096:biosynthesis; D058408:Th1-Th2 Balance; D000098815:T-bet Transcription Factor
chemical_list: D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D007074:Immunoglobulin G; D010446:Peptide Fragments; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D000217:Acyltransferases; C121433:antigen 85B, Mycobacterium tuberculosis
keywords: Adjuvant; Antibody; Immunization; MHC-II; Peptide-25; Th2