title: [Effect of andrographolide on Candida albicans biofilm dispersion].
abstract: Along with the increase in fungal infections, Candida albicans prevention and control become the focus of anti-fungal infection at present. This study aims to discuss the effect monomer andrographolide (AG) on C. albicans biofilm dispersion. In the experiment, micro-well plates and medical catheter pieces were used to establish the C. albicans biofilm model. It was discovered by XTT assay and flat band method that 1 000, 500, 250 mg x L(-1) AG could impact the activity of C. albicans biofilm dispersion cells. The morphological structures of residual biofilms on catheter pieces were observed with scanning electron microscopy, which showed that 1 000, 500, 250 mg x L(-1) AG could induce C. albicans biofilm dispersion in a dose-dependent manner, and the dispersed cells were dominated by the yeast phase. According to the real-time fluorescence quantification PCR (qRT-PCR) test, AG could up-regulate HSP90 expression and down-regulate UME6 and PES1 expressions. This study demonstrates that AG could induce C. albicans biofilm dispersion to some extent.
authors.forename: Gao-Xiang
authors.initials: GX
authors.lastname: Shi
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Yan
authors.forename: Jing
authors.initials: J
authors.lastname: Shao
authors.forename: Ke-Qiao
authors.initials: KQ
authors.lastname: Lu
authors.forename: Meng-Xiang
authors.initials: MX
authors.lastname: Zhang
authors.forename: Tian-Ming
authors.initials: TM
authors.lastname: Wang
authors.forename: Chang-Zhong
authors.initials: CZ
authors.lastname: Wang
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D008855:Microscopy, Electron, Scanning; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D004224:Diterpenes; D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; C030419:andrographolide