title: Genetic characterization of three novel chicken parvovirus strains based on analysis of their coding sequences.
abstract: Chicken parvovirus (ChPV) is one of the causative agents of viral enteritis. Recently, the genome of the ABU-P1 strain of ChPV was fully sequenced and determined to have a distinct genomic composition compared with that of vertebrate parvoviruses. However, no comparative sequence analysis of coding regions of ChPVs was possible because of the lack of other sequence information. In this study, we obtained the nucleotide sequences of all genomic coding regions of three ChPVs by polymerase chain reaction using 13 primer sets, and deduced the amino acid sequences from the nucleotide sequences. The non-structural protein 1 (NS1) gene of the three ChPVs showed 95.0 to 95.5% nucleotide sequence identity and 96.5 to 98.1% amino acid sequence identity to those of NS1 from the ABU-P1 strain, respectively, and even higher nucleotide and amino acid similarities to one another. The viral proteins (VP) gene was more divergent between the three ChPV Korean strains and ABU-P1, with 88.1 to 88.3% nucleotide identity and 93.0% amino acid identity. Analysis of the putative tertiary structure of the ChPV VP2 protein showed that variable regions with less than 80% nucleotide similarity between the three Korean strains and ABU-P1 occurred in large loops of the VP2 protein believed to be involved in antigenicity, pathogenicity, and tissue tropism in other parvoviruses. Based on our analysis of full-length coding sequences, we discovered greater variation in ChPV strains than reported previously, especially in partial regions of the VP2 protein.
authors.affiliation: a Avian Disease Laboratory, College of Veterinary Medicine , Chungbuk National University , Cheongju , Republic of Korea.
authors.forename: Bon-Sang
authors.initials: BS
authors.lastname: Koo
authors.forename: Hae-Rim
authors.initials: HR
authors.lastname: Lee
authors.forename: Eun-Ok
authors.initials: EO
authors.lastname: Jeon
authors.forename: Moo-Sung
authors.initials: MS
authors.lastname: Han
authors.forename: Kyeong-Cheol
authors.initials: KC
authors.lastname: Min
authors.forename: Seung-Baek
authors.initials: SB
authors.lastname: Lee
authors.forename: Yeon-Ji
authors.initials: YJ
authors.lastname: Bae
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Cho
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Mo
authors.forename: Hyuk Moo
authors.initials: HM
authors.lastname: Kwon
authors.forename: Haan Woo
authors.initials: HW
authors.lastname: Sung
authors.forename: Jong-Nyeo
authors.initials: JN
authors.lastname: Kim
authors.forename: In-Pil
authors.initials: IP
authors.lastname: Mo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017385:Sequence Homology; D013045:Species Specificity
chemical_list: D017931:DNA Primers