title: Estimation of trace metal elements in oral mucosa specimens by using SR-XRF, PIXE, and XAFS.
abstract: The effects of dissolved elements from metal dental restorations are a major concern in lesions of the oral mucosa, and the evaluation of accumulated metal elements, especially their distribution and chemical state, is essential for determining the precise effects of trace metals. In this study, X-ray fluorescence with synchrotron radiation (SR-XRF) and particle-induced X-ray emission (PIXE) were applied for distribution analysis of the trace metal elements contained in the oral mucosa, and the chemical states of the elements were estimated using X-ray absorption fine structure (XAFS) analysis. Appropriate combination of these analysis techniques, particularly SR-XRF and PIXE, to visualize the distributions of the elements in the oral mucosa allowed for the observation and evaluation of accumulated metal ions and debris. Importantly, the analyses in this study could be carried out using conventional histopathological specimens without damaging the specimens. Therefore, this method would be applicable for the detection of accumulated trace metal elements in biopsy specimens from the oral mucosa.
authors.affiliation: Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Motohiro
authors.initials: M
authors.lastname: Uo
authors.forename: Takahiro
authors.initials: T
authors.lastname: Wada
authors.forename: Daisuke
authors.initials: D
authors.lastname: Omagari
authors.forename: Kazuo
authors.initials: K
authors.lastname: Komiyama
authors.forename: Tadahide
authors.initials: T
authors.lastname: Noguchi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Jinbu
authors.forename: Mikio
authors.initials: M
authors.lastname: Kusama
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism*; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D014131:Trace Elements / Q000032:analysis*
chemical_list: D014131:Trace Elements