title: Extension of the CD4⁺Foxp3⁺CD25(-/low) regulatory T-cell subpopulation in type 1 diabetes mellitus.
abstract: Regulatory T-cells (Treg) have a crucial role in limiting physiologic autoreactivity. Foxp3 is a master regulator transcription factor of Treg differentiation and active Treg cells express high levels of IL-2 receptor α-chain (CD25). The aim of our study was to assess the key markers of Treg cell function in type 1 diabetic (T1DM) and control subjects by flow cytometry. The proportion of CD25(-/low) cells among CD4(+)Foxp3(+) Treg cells was higher in T1DM patients that might suggest a shifted proportion of the incomplete/reserve and the fully active (CD4(+)Foxp3(+)CD25(+)) Treg cell subpopulations in T1DM, similarly to other Th1-mediated autoimmune diseases. In addition to the decreased expression of CD25 and CTLA-4 in T1DM patients, a positive correlation was observed between the CD25 expression on CD4(+) and the CTLA-4 expression in CD8(-) T-lymphocytes both in the T1DM and in the healthy control group. Our results suggest an impaired balance of CD25(+) and CD25(-/low) Treg cells in T1DM which might reflect a decreased late phase peripheral Treg activation even in patients with a mean disease duration of more than a decade.
authors.affiliation: 2nd Department of Medicine, Semmelweis University , Budapest , Hungary .
authors.forename: András
authors.initials: A
authors.lastname: Zóka
authors.forename: Gábor
authors.initials: G
authors.lastname: Barna
authors.forename: Anikó
authors.initials: A
authors.lastname: Somogyi
authors.forename: Györgyi
authors.initials: G
authors.lastname: Műzes
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Oláh
authors.forename: Zahra
authors.initials: Z
authors.lastname: Al-Aissa
authors.forename: Orsolya
authors.initials: O
authors.lastname: Hadarits
authors.forename: Katalin
authors.initials: K
authors.lastname: Kiss
authors.forename: Gábor
authors.initials: G
authors.lastname: Firneisz
mesh_terms: D000328:Adult; D015704:CD4 Antigens / Q000378:metabolism; D016516:CD4-CD8 Ratio; D060908:CTLA-4 Antigen / Q000378:metabolism; D015897:Comorbidity; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000453:epidemiology / Q000276:immunology* / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D018655:Lymphocyte Count; D008297:Male; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D060908:CTLA-4 Antigen; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D053645:Interleukin-2 Receptor alpha Subunit; D018819:Dipeptidyl Peptidase 4
keywords: Autoreactivity; CD25; Foxp3; immune regulation; insulitis