title: [Regulation of naotai recipe on the expression of HIF-lα/VEGF signaling pathway in cerebral ischemia/reperfusion rats].
abstract: OBJECTIVE
  To observe the therapeutic angiogenesis effect of naotai recipe (NR) on local ischemia/reperfusion (I/R) injury of rats by observing signaling pathway of hypoxia-inducible factor-lα (HIF-1α) and vascular endothelial growth factor (VEGF).
  
  
  METHODS
  Totally 120 Sprague-Dawley (SD) rats were randomly divided into 4 groups, namely, the normal control group (n =12), the sham-operation group (n =12), the I/R model group (n =48), and the NR group (n =48). Cerebral I/R injury models were established using thread suture method. Rats in the I/R model group and the NR group were sub-divided into 4 sub-groups according to the 1st, 3rd, 5th, and 7th I/R day (n =12). The phenomenon of neovasculization was observed by immunofluorescence staining. The protein and mRNA expression levels of HIF-la, VEGF-A, and VEGFR II receptor were detected by RT-PCR.
  
  
  RESULTS
  There were a large amount of labels for neovasculization in the ischemic area of the NR group. Double-immunofluorescence labeling [vWF (red) and BrdU (green)] was observed in the NR group. Compared with the model group, the HIF-1α protein expression was obviously enhanced on the 1 st day of I/R (P <0.01), and the VEGF protein expression started to enhance on the 3rd day in the NR group (P <0.01). The VEGFR protein expression level was the highest in the NR group on the 5th day of I/R (P <0.01). The protein expression of VEGF and HIF-1α started to decrease on the 7th day of I/R.
  
  
  CONCLUSIONS
  NR could strengthen angiogenesis after I/R by elevating the expression of HIF-lα and activating HIF-lα/VEGF signaling pathway.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.forename: Hui-bin
authors.initials: HB
authors.lastname: Zhu
authors.forename: Jun
authors.initials: J
authors.lastname: Liao
authors.forename: Ya-qiao
authors.initials: YQ
authors.lastname: Yi
authors.forename: Guo-zuo
authors.initials: GZ
authors.lastname: Wang
authors.forename: Le
authors.initials: L
authors.lastname: Tong
authors.forename: Jin-wen
authors.initials: JW
authors.lastname: Ge
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism*; D002544:Cerebral Infarction; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism; D007511:Ischemia; D009389:Neovascularization, Pathologic; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D042461:Vascular Endothelial Growth Factor A