title: [Characteristics of migration of adipose tissue derived mesenchymal stromal cells after co-cultivation with activated monocytes in vitro].
abstract: Mesenchymal stromal cells (MSC) are considered to be promising tool of regenerative medicine. Migration of MSC toward damaged inflammatory site is essential for physiological tissue reparation. Therefore we studied modifications of migratory features of adipose tissue derived MSC (AT-MSC) after co-cultivation with activated monocytes derived from THP-1 cell line. As a result, we have observed an increased migration rate of AT-MSC in vitro in the absence of chemoattractant gradient as well as toward the gradient of PDGF BB (platelet-derived growth factor BB), which is well known chemoattractant for the cells of mesenchymal origin. Furthermore, the rate of directional AT-MSC migration through fibronectin was also increased. We have established that signaling from PDGFRβ which is activated through binding of integrin receptors with extracellular matrix may be possible way to stimulate cellular migration under simulated inflammatory conditions.
authors.forename: O A
authors.initials: OA
authors.lastname: Grigor'eva
authors.forename: I V
authors.initials: IV
authors.lastname: Korovina
authors.forename: B Sh
authors.initials: BSh
authors.lastname: Gogia
authors.forename: V Iu
authors.initials: VIu
authors.lastname: Sysoeva
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism; D000328:Adult; D000077214:Becaplermin; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D018920:Coculture Techniques; D005109:Extracellular Matrix / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D039001:Integrin alpha Chains / Q000235:genetics / Q000378:metabolism; D039641:Integrin beta Chains / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011485:Protein Binding; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000819:agonists / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D039001:Integrin alpha Chains; D039641:Integrin beta Chains; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020797:Receptor, Platelet-Derived Growth Factor beta; D013755:Tetradecanoylphorbol Acetate