title: Identification and expression analysis of cDNA encoding chitinase-like protein (CLP) gene in Japanese scallop Mizuhopecten yessoensis.
abstract: Chitinase-like proteins (CLP) are important members of the glycoside hydrolase family 18 (GH18) and are involved in growth control and remodeling processes. In this study, a CLP transcript was isolated and sequenced from the Japanese scallop (Mizuhopecten yessoensis) after screening expressed sequence tags. The full-length complementary DNA of M. yessoensis CLP (My-Clp1) was 1555 bp in length, consisting of a 75-bp 5'-untranslated region (UTR), a 160-bp 3'-UTR, and a 1320-bp open reading frame bearing characteristics of the GH18 family. The My-Clp1 protein was well conserved, with similar domain structures and architecture across species (e.g., from mollusks to mammals). Expression analysis in healthy tissues and across developmental stages revealed a strong preference for expression; My-Clp1 was abundantly expressed in the mantle and throughout metamorphosis, which suggests the involvement of My-Clp1 in the synthesis of extracellular components, and tissue degeneration and remodeling. My-Clp1 expression was induced after infection with a bacterial pathogen, Vibrio anguillarum, suggesting its involvement in immunity against this intracellular pathogen.
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: L
authors.initials: L
authors.lastname: Gao
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Xu
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: H
authors.initials: H
authors.lastname: Su
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Gao
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: X B
authors.initials: XB
authors.lastname: Bao
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China.
authors.forename: W D
authors.initials: WD
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Fishery Molecular Biology of Liaoning Province, Liaoning Ocean and Fisheries Science Research Institute, Dalian, China hechongbo@hotmail.com.
authors.forename: C B
authors.initials: CB
authors.lastname: He
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002688:Chitinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D049897:Pectinidae / Q000382:microbiology / Q000502:physiology*; D010802:Phylogeny; D059467:Transcriptome / Q000502:physiology*; D014733:Vibrio / Q000472:pathogenicity; D014735:Vibrio Infections / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D002688:Chitinases