title: Commensal Streptococcus mitis is a unique vector for oral mucosal vaccination.
abstract: The development of vaccine approaches that induce mucosal and systemic immune responses is critical for the effective prevention of several infections. Here, we report on the use of the abundant human oral commensal bacterium Streptococcus mitis as a delivery vehicle for mucosal immunization. Using homologous recombination we generated a stable rS. mitis expressing a Mycobacterium tuberculosis protein (Ag85b). Oral administration of rS. mitis in gnotobiotic piglets resulted in efficient oral colonization and production of oral and systemic anti-Ag85b specific IgA and IgG antibodies. These results support that the commensal S. mitis is potentially a useful vector for mucosal vaccination.
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states.
authors.forename: Nada
authors.initials: N
authors.lastname: Daifalla
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Cayabyab
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states.
authors.forename: Emily
authors.initials: E
authors.lastname: Xie
authors.affiliation: Cummings School of Veterinary Medicine at Tufts, Grafton, MA, United states; Department of Animal Resources Science at Dankook University, Cheonan, South Korea.
authors.forename: Hyeun Bum
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Cummings School of Veterinary Medicine at Tufts, Grafton, MA, United states.
authors.forename: Saul
authors.initials: S
authors.lastname: Tzipori
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states.
authors.forename: Philip
authors.initials: P
authors.lastname: Stashenko
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states.
authors.forename: Margaret
authors.initials: M
authors.lastname: Duncan
authors.affiliation: The Forsyth Institute, Cambridge, MA, United states. Electronic address: acampos@forsyth.org.
authors.forename: Antonio
authors.initials: A
authors.lastname: Campos-Neto
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001428:Bacterial Vaccines / Q000276:immunology*; D005822:Genetic Vectors / Q000276:immunology; D009092:Mucous Membrane; D009169:Mycobacterium tuberculosis; D065568:Oral Mucosal Absorption / Q000276:immunology*; D034361:Streptococcus mitis / Q000235:genetics / Q000276:immunology*; D013552:Swine / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines
keywords: Ag85b; Gnotobiotic piglets; Mucosal vaccine; Oral immunization; Streptococcus mitis; Vaccine vector