title: Cytosolic dsDNA triggers apoptosis and pro-inflammatory cytokine production in normal human melanocytes.
abstract: Considerable evidence implicates that viral infection might be a participant factor in the pathogenesis of vitiligo. However, it is still unclear how viral infection leads to the melanocyte destruction. To elucidate the effects of viral dsDNA on the viability and cytokine synthesis of normal human melanocytes and to explore the underlying mechanisms, primary cultured normal human melanocytes were transfected with poly(dA:dT). The results demonstrated that poly(dA:dT) triggered apoptosis instead of pyroptosis in melanocytes. Knocking down AIM2 or RIG-I by RNA interference partially reduced the poly(dA:dT)-induced LDH release, suggesting the involvement of both nucleic acid sensors in the process of melanocyte death. Poly(dA:dT) induced the expression of pro-inflammatory cytokine genes including IFN-β, TNF-α, IL-6 and IL-8 as well, whereas the pro-inflammatory cytokine production was suppressed by RIG-I siRNA, but not by AIM2 siRNA. Poly(dA:dT) treatment increased the phosphorylation of p38 and JNK and NFκB. Accordingly, NFκB inhibitor Bay 11-7082 and JNK inhibitor SP600125 blocked the induction of the cytokine genes except IFN-β. The production of IL6 and IL8 was also suppressed by p38 inhibitor SB203580. On the contrary, the Poly(dA:dT)-induced melanocyte death was only decreased by SP600125. This study provides the possible mechanism of melanocyte destruction and immuno-stimulation in vitiligo by innate immune response following viral infection.
authors.affiliation: Department of Dermatology, Third People's Hospital of Hangzhou, Hangzhou, Zhejiang Province, China.
authors.forename: Suiquan
authors.initials: S
authors.lastname: Wang
authors.forename: Dongyin
authors.initials: D
authors.lastname: Liu
authors.forename: Weixuan
authors.initials: W
authors.lastname: Ning
authors.forename: Aie
authors.initials: A
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D003600:Cytosol / Q000276:immunology / Q000821:virology; D004279:DNA, Viral / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D018836:Inflammation Mediators / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000276:immunology; D008544:Melanocytes / Q000166:cytology* / Q000276:immunology* / Q000821:virology; D016328:NF-kappa B / Q000276:immunology; D011067:Poly dA-dT / Q000276:immunology; D014777:Virus Diseases / Q000150:complications / Q000276:immunology; D014820:Vitiligo / Q000209:etiology / Q000276:immunology / Q000473:pathology
chemical_list: D016207:Cytokines; D004279:DNA, Viral; D018836:Inflammation Mediators; D016328:NF-kappa B; D011067:Poly dA-dT
keywords: dsDNA; innate immune response; melanocyte; poly (dA:dT); vitiligo