title: The role of the Cronobacter sakazakii ProP C-terminal coiled coil domain in osmotolerance.
abstract: BACKGROUND
  We investigate the role of the C-terminal coiled coil of the secondary proline porter ProP in contributing to Cronobacter sakazakii osmotolerance.
  
  
  RESULTS
  The extended C-terminal domain of ProP1 (encoded by ESA_02131) was spliced onto the truncated C-terminal end of ProP2 (encoded by ESA_01706); creating a chimeric protein (ProPc) which exhibits increased osmotolerance relative to the wild type.
  
  
  CONCLUSIONS
  It appears that the C-terminal coiled coil domain tunes ProP at low osmolality, whereas ProP transporters lacking the coiled coil domain are more active at a higher osmolality range.
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Audrey
authors.initials: A
authors.lastname: Feeney
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Johnston
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Alan
authors.initials: A
authors.lastname: Lucid
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Jim
authors.initials: J
authors.lastname: O'Mahony
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Aidan
authors.initials: A
authors.lastname: Coffey
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Brigid
authors.initials: B
authors.lastname: Lucey
authors.affiliation: Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork Ireland.
authors.forename: Roy D
authors.initials: RD
authors.lastname: Sleator