title: Morphometric analysis of epithelial thickness and blood vessels in different grades of oral submucous fibrosis.
abstract: BACKGROUND
  Oral submucous fibrosis (OSF) is a common oral health problem in the Indian subcontinent. It is characterized by a juxtaepithelial inflammatory reaction followed by fibroelastic changes in the lamina propria. Traditionally, it is said to be associated with marked epidermal atrophy and decreased vasculature as the disease advances.
  
  
  OBJECTIVE
  To assess the changes in epidermal thickness and mucosal vasculature in various stages of the disease.
  
  
  METHODS
  Patients with histological diagnosis of OSF were included in the study. Demographic data and oral habits of each patient were collected. The severity of OSF was graded histologically according to Pindborg and Sirsat. Epithelial thickness and subepithelial blood vessel area, diameter and perimeter were measured and analysed using Image analysis software IMAGE PRO PLUS version 6.0.
  
  
  RESULTS
  Thirty-five patients with OSF were studied. 25 (71.4%) were males and 10 (28.6%) were females with a male to female ratio of 1.3:1. Most patients were in the 31-40 yrs age group. The majority of patients (40%) chewed areca nut/dohra. Each grade of the disease was found to display either hyperplastic or atrophic epithelial changes. The mean blood vessel area, diameter and perimeter did not show any sustained change with the increasing severity (grade) of the disease.
  
  
  CONCLUSIONS
  These findings question the role of ischaemia in the aetiopathogenesis of oral submucous fibrosis.
authors.affiliation: Fortis Memorial Research Institute, Gurgaon, India. xs2neerajgarg@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Garg
authors.forename: R
authors.initials: R
authors.lastname: Mehrotra R
mesh_terms: D000328:Adult; D001808:Blood Vessels / Q000473:pathology*; D004848:Epithelium / Q000098:blood supply / Q000473:pathology; D005260:Female; D005355:Fibrosis / Q000453:epidemiology / Q000473:pathology; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D009059:Mouth Diseases / Q000453:epidemiology* / Q000473:pathology*; D009061:Mouth Mucosa / Q000098:blood supply / Q000473:pathology*; D009092:Mucous Membrane / Q000098:blood supply / Q000473:pathology*