title: Genetic diversity and relationships in cultivars of Lolium multiflorum Lam. using sequence-related amplified polymorphism markers.
abstract: Sequence-related amplified polymorphism (SRAP) markers were used to analyze and estimate the genetic variability, level of diversity, and relationships among 20 cultivars and strains of annual ryegrass (Lolium multiflorum Lam.). Eighteen SRAP primer combinations generated 334 amplification bands, of which 298 were polymorphic. The polymorphism information content ranged from 0.4715 (me10 + em1) to 0.5000 (me5 + em7), with an average of 0.4921. The genetic similarity coefficient ranged from 0.4304 to 0.8529, and coefficients between 0.65 and 0.90 accounted for 90.00%. The cluster analysis separated the accessions into five groups partly according to their germplasm resource origins.
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: L K
authors.initials: LK
authors.lastname: Huang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.
authors.forename: Q T
authors.initials: QT
authors.lastname: Huang
authors.affiliation: Entomology and Nematology, University of Florida, Apoke, FL, USA.
authors.forename: Y F
authors.initials: YF
authors.lastname: Xiao
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China czh@swun.edu.cn.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Chen
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: J M
authors.initials: JM
authors.lastname: Miao
authors.affiliation: Department of Grassland Science, Animal Science and Technology College, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: H D
authors.initials: HD
authors.lastname: Yan
mesh_terms: D016000:Cluster Analysis; D005819:Genetic Markers; D018745:Genome, Plant; D008129:Lolium / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers