title: [Comparative analysis of the promoting blood effects of the combination of different proportions of danggui and honghua by the principal component analysis and multi-attribute comprehensive index methods].
abstract: The combination of Danggui and Honghua (GH) is a popular herb pair commonly used in clinic for the treatment of blood stasis syndrome in China. To evaluate the activating blood circulation and dissipating blood stasis effects of the combination of different proportions of Danggui and Honghua on acute blood stasis rats, and optimize the proportion of GH to have the best activating blood circulation and dissipating blood stasis effect. Acute blood stasis rat model was induced by subcutaneous injection of adrenaline and ice water bath. The blood stasis rats were administrated intragastrically with GH (1 : 0, 4 : 1, 2 : 1, 3 : 2, 1 : 1, 2 : 3, 1: 2, 1 : 4 and 0 : 1) extracts. The whole blood viscosity (WBV), plasma viscosity (PV), and high shear whole blood relative index (HSWBRI), low shear whole blood relative index (LSWBRI), and erythrocyte aggregation index (EAI) were tested to observe the effects of GH on hemorheology of blood stasis rats. And the maximum aggregation induced by adenosine diphosphate (ADP) was tested to observe the effect of GH on platelet aggregation index of blood stasis rats. In addition, the prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT) and plasma fibrinogen (FIB) were tested to observe the effects of GH on blood coagulation function of blood stasis rats. Then principal component analysis and multi-attribute comprehensive index methods were both used to comprehensively evaluate the total activating blood circulation and dissipating blood stasis effects of GH. The results showed that the hemorheological indexes and coagulation parameters of model group both had significant differences with normal group. Compared with model group, GH (1 : 0, 4 : 1, 2: 1, 3 : 2, 1 : 1, 2 : 3, 1 : 2, 1 : 4 and 0 : 1) could improve all the blood hemorheology indexes and regulate part indexes of blood coagulation function and platelet aggregation in acute blood stasis rats. Based on principal component analysis and multi-attribute comprehensive index methods, GH 1 : 1 and GH 3 : 2 both had the best effect of blood circulation and dissipating blood stasis, and the effect of GH 1 : 1 was slightly better than GH 3 : 2. These results suggest that GH could obviously ameliorate the abnormality of hemorheology and blood coagulation function in acute blood stasis rats. The optimized proportion of GH was consistent with regulations of medicine usage that GH 1 : 1 had the highest frequency used in traditional Chinese formulae. It could provide scientific basis for more effective application of the compatibility between Danggui and Honghua in modern clinic medicine.
authors.forename: Shu-Jiao
authors.initials: SJ
authors.lastname: Li
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Li
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D029971:Angelica sinensis; D000818:Animals; D001777:Blood Coagulation; D001809:Blood Viscosity; D031187:Carthamus tinctorius; D002681:China; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004903:Erythrocyte Aggregation; D018056:Hemorheology; D010314:Partial Thromboplastin Time; D010974:Platelet Aggregation; D025341:Principal Component Analysis; D011517:Prothrombin Time; D051381:Rats; D017207:Rats, Sprague-Dawley; D013918:Thrombin Time
chemical_list: D004365:Drugs, Chinese Herbal; C079426:Honghua extract, Carthamus tinctorius; C044613:angelicae sinensis extract