title: Spectroscopic study on the interaction between mononaphthalimide spermidine (MINS) and bovine serum albumin (BSA).
abstract: The interaction mononaphthalimide spermidine (MINS, 1) and bovine serum albumin (BSA) was studied by UV/vis absorption, fluorescence and circular dichroism spectra (CD) under physiological conditions (pH=7.4). The observed spectral quenching of BSA by compound 1 indicated compound 1 could bind to BSA. Further fluorescent tests revealed that the quenching mechanism of BSA by compound 1 was overall static. Meanwhile, the obtained binding constant and thermodynamic parameters on compound-BSA interaction showed that the type of interaction force of compound 1 and BSA was mainly hydrophobic. The analysis of synchronous, three-dimensional fluorescence and CD showed that compound 1 had weak influence on the conformational changes in BSA. Molecular docking simulation was performed and docking model in silico suggested that the configuration of compound 1 was localized in enzymatic drug site II in BSA. Furthermore, naphthalimide moiety of compound 1 greatly contributed to the hydrophobic interaction between compound 1 and BSA protein, as confirmed by experimental data.
authors.affiliation: Institute of Chemical Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Tian
authors.affiliation: Institute of Chemical Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Fenglei
authors.initials: F
authors.lastname: Zang
authors.affiliation: The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Luo
authors.affiliation: Institute of Chemical Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Institute of Chemical Biology, Henan University, Kaifeng 475004, PR China.
authors.forename: Yueqiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Huaihe Clinical Institute, Henan University, Kaifeng 475004, PR China. Electronic address: 1322078049@qq.com.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, PR China. Electronic address: wcjsxq@henu.edu.cn.
authors.forename: Chaojie
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013095:Spermidine / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics
chemical_list: D012710:Serum Albumin, Bovine; D013095:Spermidine
keywords: Bovine serum albumin (BSA); Molecular docking; Mononaphthalimide spermidine; Spectroscopic methods