title: Twenty natural amino acids identification by a photochromic sensor chip.
abstract: All 20 natural amino acids identification shows crucial importance in biochemistry and clinical application while it is still a challenge due to highly similarity in molecular configuration of the amino acids. Low efficiency, complicated sensing molecules and environment hindered the successful identification. Here, we developed a facile sensor chip composed of one photochromic molecule with metal ions spotted to form spirooxazine-metallic complexes, and successfully recognized all the 20 natural amino acids as well as their mixtures. The sensor chip gives distinct fluorescent fingerprint pattern of each amino acid, based on multistate of spirooxazine under different light stimulations and discriminated interaction between various metal ions and amino acids. The sensor chip demonstrates powerful capability of amino acids identification, which promotes sensing of biomolecules.
authors.affiliation: Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Qin
authors.forename: Fengyu
authors.initials: F
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.forename: Wei
authors.initials: W
authors.lastname: Ran
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.forename: Yanlin
authors.initials: Y
authors.lastname: Song
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D004396:Coloring Agents / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008670:Metals / Q000737:chemistry*; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013141:Spiro Compounds / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D004396:Coloring Agents; D005456:Fluorescent Dyes; D008670:Metals; D013141:Spiro Compounds