title: [Biochip for determination of genetic markers of sporadic Alzheimer's disease in the Russian Slavic population].
abstract: A biological microchip (biochip) for the genetic predis- position to sporadic form of Alzheimer's disease studying has been developed. The biochip allows determina- tion of ten genetic polymorphisms within APOE, TOMM40, APOJ, EXOC3L2, GAB2, A2M, CR1, BIN1 and PICALM genes. The genotyping assay includes the amplification of loci of interest and further allele-specific hybridization of the fluorescent labeled amplicons with oligonucleotides immobilized on a biochip. Based on the results of genotyping of 166 patients and 128 controls APOE epsilon4 allele was found to be significantly associated with Alzheimer's disease susceptibility (OR = 2.275, 95% CI = 1.045-4.954,p = 0.034). Additionally, protective effects for the APOE epsilon2 allele and CLUT-allele (rs11136000) were observed (OR = 0.215, 95% CI = 0.090-0.516, p = 0.001 and OR = 0.679, 95% CI = 0.47-0.99, p = 0.042, respectively). Gene-gene interaction revealed two genotype combinations associated with Alzheimer's disease: APOE E3/E4 GAB2 G/G (OR = 2.49; CI = 1.43-4.32, p = 0.001) and APOE epsilon4 GAB2 G/G (OR = 3.55, CI = 1.23-10.24,p = 0.015). Based on the results of the combined multivariate analysis the algorithm for identifying of individuals at increased risk of Alzheimer's disease was developed.
authors.forename: I I
authors.initials: II
authors.lastname: Nizamutdinov
authors.forename: T V
authors.initials: TV
authors.lastname: Andreeva
authors.forename: V A
authors.initials: VA
authors.lastname: Stepanov
authors.forename: A V
authors.initials: AV
authors.lastname: Marusin
authors.forename: E I
authors.initials: EI
authors.lastname: Rogaev
authors.forename: A S
authors.initials: AS
authors.lastname: Zasedatelev
authors.forename: T V
authors.initials: TV
authors.lastname: Nasedkina
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D005006:Ethnicity / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D012307:Risk Factors; D012426:Russia
chemical_list: D005819:Genetic Markers