title: Mechanical properties and cytocompatibility of carbon fibre reinforced nano-hydroxyapatite/polyamide66 ternary biocomposite.
abstract: METHODS
  Fibre-reinforced composites with good strength and ductility as bone repair biomaterials have been attracting increasing attention in biomedical applications. In the present study, a novel ternary composite was prepared using carbon fibre (CF) to reinforce a nano-hydroxyapatite/polyamide66 composite (HA/PA). The interface and mechanical strength of the ternary composite (CF/HA/PA) were characterised. In addition, to assess the cytocompatibility, the composite was co-cultured with MG-63 cells, and the cell morphology, MTT, and ALP were tested.
  
  
  RESULTS
  The results indicated that CFs were uniformly distributed in the HA/PA matrix with random orientation and that the CFs bonded well to the HA/PA matrix. The reinforced ternary composite exhibited a compressive strength of 116-212 MPa, a bending strength of 89-138 MPa, a tensile strength of 109-181 MPa, with the breaking elongation ratio of 6.2-9.1%, and a tensile modulus of 2.9-5.8 GPa, with the values varying with increasing CF content from 5 to 20 (mass fraction). The MG-63 cells of normal phenotype were well extended and spread onto the ternary composite surface. In addition, its proliferation and differentiation on the composite surface were significantly increased with time, indicating that the incorporation of CFs into HA/PA had little negative effects on MG-63 cells.
  
  
  CONCLUSIONS
  The incorporation of CFs into a HA/PA66 composite improved the strength and ductility and introduced no negative effects on the cytocompatibility. Hence, the CF/HA/PA ternary composite has potential to be used as a bone repair materials and in fixation devices.
authors.affiliation: The General Hospital of PLA, Beijing, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The General Hospital of PLA, Beijing, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: China-Japanese Friendship Hospital, Beijing, China. Electronic address: xuelianzhang2014@sina.com.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The General Hospital of PLA, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The General Hospital of PLA, Beijing, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
authors.affiliation: The General Hospital of PLA, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lu
authors.affiliation: College of Physical Science and Technology, Sichuan University, Chengdu, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D055595:Mechanical Phenomena; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D000077482:Carbon Fiber; D009757:Nylons; D002244:Carbon; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Carbonate fibre; Cytocompatibility; HA/PA66 composite; Mechanical properties