title: Oxymatrine targets EGFR(p-Tyr845) and inhibits EGFR-related signaling pathways to suppress the proliferation and invasion of gastric cancer cells.
abstract: OBJECTIVE
  Oxymatrine (matrine oxide, matrine N-oxide, matrine 1-oxide) is one of the quinolizidine alkaloid compounds extracted from the root of Sophora flavescens (a traditional Chinese herb). Oxymatrine has been known for its chemoresistance and cytotoxic effects on various cancer cells, but the mechanism underlying has not been explored. We study the mechanism of oxymatrine on gastric cells.
  
  
  METHODS
  We observed the changes of proliferation, apoptosis and invasion in human gastric cells by detecting the signaling pathway in which oxymatrine plays role.
  
  
  RESULTS
  These results showed that oxymatrine inhibited the proliferation and invasion of gastric cells through inhibition of EGFR/Cyclin D1/CDK4/6, EGFR/Akt and MEK-1/ERK1/2/MMP2 pathway by inhibiting EGFR(p-Tyr845). In addition to inducing gastric cells apoptosis, oxymatrine significantly inhibited the migration and invasion of human gastric cancer cells by decreasing phospho-Cofilin (Ser3) and phospho-LIMK1 (Thr508) without changing the total Cofilin and LIMK1 expression.
  
  
  CONCLUSIONS
  Oxymatrine effectively suppressed the phosphorylation of EGFR (Tyr845), and EGFR was the target of oxymatrine.
authors.affiliation: Institute of Neurology, General Hospital of Shenyang Military Command, 83#Wenhua Road, Shenhe District, Shenyang, 110016, Liaoning, China.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Guo
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Su
authors.forename: Kaiwen
authors.initials: K
authors.lastname: Wang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D066246:ErbB Receptors / Q000037:antagonists & inhibitors*; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D011807:Quinolizines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000473:pathology*; D014443:Tyrosine / Q000187:drug effects; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D066246:ErbB Receptors; D011807:Quinolizines; D014443:Tyrosine; C037573:oxymatrine; D000093842:Matrines