title: Proteomic changes during tuber dormancy release process revealed by iTRAQ quantitative proteomics in potato.
abstract: Given that limited information is available with regard to tuber dormancy release related proteome, we conducted proteome analysis of tuber dormancy release process at dormant tuber (DT), dormancy release tuber (DRT) and sprouting tuber (ST) using the iTRAQ technology. A total of 1,752 proteins were identified. Among them, a subset of 316 proteins was screened as significant up- (137) and down regulated (179) between DT vs DRT. A subset of 120 proteins experienced significant up- (40) or down-regulation (80) between DRT vs ST. The differentially expressed proteins were grouped into 11 functional categories. Proteins enriched in functional categories of major carbohydrate (CHO) metabolism, glycolysis, fermentation, amino acid metabolism, protein and transport were highly up-regulated, while functional categories of photosynthesis and RNA were down-regulated between DT vs DRT. Proteins enriched in functional groups of protein, cell wall, lipid metabolism, miscellaneous, and signaling were strongly up-regulated, while functional categories of photosynthesis, hormone metabolism and protein were down-regulated between DRT vs ST. Consistent with previous documented differentially expressed genes, most of differentially expressed proteins were also identified between DT and DRT, indicating the metabolism shift from growth suspension to growth activation as tubers dormancy breaking. The changes in protein profiles showed lower concordance with corresponding alterations in transcript levels, indicating possible transcriptional and posttranscriptional regulation. Furthermore, the possible mechanism of tuber dormancy release was discussed in relation to what was known in transcripts change and other plant models from carbohydrate metabolism, protein metabolism, stress response, redox regulation, transcription regulation, DNA metabolism, amino acid metabolism, development, signaling as well as hormone metabolism.
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Bailin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB E3B 6C2, Canada.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chang
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: hjsi@gsau.edu.cn.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Si
authors.affiliation: Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, People's Republic of China; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, People's Republic of China. Electronic address: wangd@gsau.edu.cn.
authors.forename: Di
authors.initials: D
authors.lastname: Wang
mesh_terms: D016000:Cluster Analysis; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry / Q000379:methods; D057445:Plant Dormancy; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D035281:Plant Tubers / Q000378:metabolism*; D020543:Proteome / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D012333:RNA, Messenger
keywords: Dormancy; Potato tuber; Proteome; Sprouting; iTRAQ