title: A sensitive biosensor for the fluorescence detection of the acetylcholinesterase reaction system based on carbon dots.
abstract: The carbon dots (C-dots) with high fluorescence quantum yield were prepared using hydrothermal method. C-dots have been adopted as probes for the fluorescence turn-off detection of H2O2 based on the special sensibility for the hydroxyl radical. And then the biosensors for the detection of substrate and enzymes activities were established in the acetylcholinesterase reaction system, which were related to the production of H2O2. Specifically, the proposed fluorescent biosensor was successfully applied to detect the concentration of choline (in the range from 0.025 to 50 μM) and acetylcholine (in the range from 0.050 to 50 μM), and the activity of choline oxidase (in the range from 1 to 75 U/L) and acetylcholinesterase (1 to 80 U/L). These results showed a sensitive, universal, nontoxic and eco-friendly detecting technique has been developed.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China; The State Key Laboratory of Bioelectronics, Southeast University, 210096, PR China.
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Wei
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ren
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Qiang
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China. Electronic address: tangfq@mail.ipc.ac.cn.
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, NO. 29, Zhongguancun East Road, Haidian District, Beijing 100190, PR China. Electronic address: mengxw@mail.ipc.ac.cn.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase / Q000737:chemistry*; D000429:Alcohol Oxidoreductases / Q000032:analysis*; D015374:Biosensing Techniques; D002021:Buffers; D002244:Carbon / Q000737:chemistry*; D002794:Choline / Q000032:analysis*; D005453:Fluorescence; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D057230:Limit of Detection; D045663:Quantum Dots / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence
chemical_list: D002021:Buffers; D012996:Solutions; D017665:Hydroxyl Radical; D002244:Carbon; D006861:Hydrogen Peroxide; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; D002794:Choline; D000109:Acetylcholine
keywords: Acetylcholinesterase; Biosensor; Carbon dots; Fluorescence; Hydrogen peroxide