title: A potential role for regulatory T-cells in the amelioration of DSS induced colitis by dietary non-digestible polysaccharides.
abstract: Inflammatory bowel diseases (IBD) including ulcerative colitis (UC) and Crohn's disease (CD) are chronic relapsing inflammatory disorders of the gastrointestinal tract. The interaction between a disturbed microbial composition, the intestinal mucosal barrier and the mucosal immune system plays an important role in IBD and its chronicity. It has been indicated that due to the altered microbial composition the balance between T regulatory cells (Treg) and T helper cells (Th) 17 is disturbed, leading to an inflammatory state. The present study shows that oral intake of a specific multi fibre mix (MF), designed to match the fibre content of a healthy diet, counteracts IBD-like intestinal inflammation and weight loss in dextran sodium sulphate treated mice. This reduction in inflammation might be brought about, at least in part, by the MF-induced decrease in inflammatory cytokines, increase in IL-10 and the relative increase in Treg cells in the mesenteric lymph nodes (MLN). Moreover, the Treg percentage in the MLN correlates with the percentage of tolerogenic lamina propria derived CD103+RALDH+dendritic cells in the MLN, suggesting that these play a role in the observed effects. In children with CD exclusive enteral nutrition (EEN) is a widely used safe and effective therapy. Optimizing enteral nutritional concepts with the tested fibre mix, know to modulate the gut microbiota composition, SCFA production and inflammatory status (as indicated by the present study) could possibly further improve efficacy in inducing remission.
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands. Electronic address: anita.hartog@danone.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Hartog
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands.
authors.forename: Fabiën N
authors.initials: FN
authors.lastname: Belle
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Bastiaans
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands.
authors.forename: Priscilla
authors.initials: P
authors.lastname: de Graaff
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors.forename: Johan
authors.initials: J
authors.lastname: Garssen
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands.
authors.forename: Lucien F
authors.initials: LF
authors.lastname: Harthoorn
authors.affiliation: Nutricia Research, Uppsalalaan 12, 3583 CT Utrecht, The Netherlands; Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors.forename: Arjan P
authors.initials: AP
authors.lastname: Vos
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D003106:Colon / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000097:blood / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D018928:Immunity, Mucosal; D056747:Immunomodulation; D015212:Inflammatory Bowel Diseases / Q000178:diet therapy* / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008640:Mesenteric Lymphadenitis / Q000209:etiology / Q000517:prevention & control; D008810:Mice, Inbred C57BL; D056692:Prebiotics / Q000032:analysis; D011897:Random Allocation; D000685:Serum Amyloid A Protein / Q000032:analysis / Q000037:antagonists & inhibitors; D012995:Solubility; D050378:T-Lymphocytes, Regulatory / Q000276:immunology* / Q000378:metabolism; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D016207:Cytokines; D056692:Prebiotics; D000685:Serum Amyloid A Protein; D016264:Dextran Sulfate
keywords: CD103; IBD; Multifibre; Prebiotic; RALDH; Treg