title: Enhanced cadaverine production from L-lysine using recombinant Escherichia coli co-overexpressing CadA and CadB.
abstract: The effect of fusing the PelB signal sequence to lysine/cadaverine antiporter (CadB) on the bioconversion of L-lysine to cadaverine was investigated. To construct a whole-cell biocatalyst for cadaverine production, four expression plasmids were constructed for the co-expression of lysine decarboxylase (CadA) and lysine/cadaverine antiporter (CadB) in Escherichia coli. Expressing CadB with the PelB signal sequence increased cadaverine production by 12%, and the optimal expression plasmid, pETDuet-pelB-CadB-CadA, contained two T7 promoter-controlled genes, CadA and the PelB-CadB fusion protein. Based on pETDuet-pelB-CadB-CadA, a whole-cell system for the bioconversion of L-lysine to cadaverine was constructed, and three strategies for L-lysine feeding were evaluated to eliminate the substrate inhibition problem. A cadaverine titer of 221 g l(-1) with a molar yield of 92% from lysine was obtained.
authors.affiliation: State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, People's Republic of China, will1015@hotmail.com.
authors.forename: Weichao
authors.initials: W
authors.lastname: Ma
authors.forename: Weijia
authors.initials: W
authors.lastname: Cao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Kequan
authors.initials: K
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Pingkai
authors.initials: P
authors.lastname: Ouyang
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism*; D017920:Antiporters / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017123:Bacteriophage T7 / Q000235:genetics; D002103:Cadaverine / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D008239:Lysine / Q000378:metabolism*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D026905:Amino Acid Transport Systems; D017920:Antiporters; D001426:Bacterial Proteins; C088101:CadB protein, Bacteria; D002262:Carboxy-Lyases; C021520:lysine decarboxylase; D008239:Lysine; D002103:Cadaverine