title: Detection of a novel single nucleotide polymorphism and imprinted status analysis of the Ras protein-specific guanine nucleotide-releasing factor 1 gene in domestic pigs.
abstract: The aim of this study was to determine the imprinting status of the Ras protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1) gene in domestic pigs. In this study, a 228-bp partial sequence located in exon 14 and a 193-bp partial sequence located in exon 1 of the Rasgrf1 gene in domestic pigs were obtained. A novel single nucleotide polymorphism, a G/A transition, was identified in Rasgrf1 exon 14, and then the reciprocal Berkshire x Wannan black F1 hybrid model and the reverse transcription-polymerase chain reaction-restriction fragment length polymorphism method were used to detect the imprinting status of the porcine Rasgrf1 gene at the 1-day-old developmental stage. Imprinting analysis showed that, compared to the imprinted expression of the Rasgrf1 gene in mouse and rat, a variable imprinting status was observed in domestic pigs. In principle, the porcine Rasgrf1 gene was maternally expressed in the liver and small intestine, paternally expressed in the lung, and biallelically expressed in brain, heart, spleen, kidney, stomach, pancreas, fat, testis, ovary, longissimus dorsi, and pituitary tissues. In conclusion, our results indicated that the Rasgrf1 gene shows both species- and tissue-specific variation in imprinted expression.
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Ding
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: X D
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Veterinary, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Zhang
authors.affiliation: Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui, China yinzongjun@ahau.edu.cn.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Yin
mesh_terms: D000818:Animals; D005091:Exons / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D018392:Genomic Imprinting; D006150:Guanine Nucleotides / Q000235:genetics; D008297:Male; D051379:Mice; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051381:Rats; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development; D014018:Tissue Distribution; D020666:ras-GRF1 / Q000235:genetics*
chemical_list: D006150:Guanine Nucleotides; D020666:ras-GRF1