title: Ancestry informative markers: inference of ancestry in aged bone samples using an autosomal AIM-Indel multiplex.
abstract: Ancestry informative markers (AIMs) can be useful to infer ancestry proportions of the donors of forensic evidence. The probability of success typing degraded samples, such as human skeletal remains, is strongly influenced by the DNA fragment lengths that can be amplified and the presence of PCR inhibitors. Several AIM panels are available amongst the many forensic marker sets developed for genotyping degraded DNA. Using a 46 AIM Insertion Deletion (Indel) multiplex, we analyzed human skeletal remains of post mortem time ranging from 35 to 60 years from four different continents (Sub-Saharan Africa, South and Central America, East Asia and Europe) to ascertain the genetic ancestry components. Samples belonging to non-admixed individuals could be assigned to their corresponding continental group. For the remaining samples with admixed ancestry, it was possible to estimate the proportion of co-ancestry components from the four reference population groups. The 46 AIM Indel set was informative enough to efficiently estimate the proportion of ancestry even in samples yielding partial profiles, a frequent occurrence when analyzing inhibited and/or degraded DNA extracts.
authors.affiliation: Forensic DNA Laboratory, Argentinean Forensic Anthropology Team (EAAF) Independencia 644,3A, 5000 Cordoba, Argentina.
authors.forename: Carola
authors.initials: C
authors.lastname: Romanini
authors.affiliation: Forensic DNA Laboratory, Argentinean Forensic Anthropology Team (EAAF) Independencia 644,3A, 5000 Cordoba, Argentina.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Romero
authors.affiliation: Forensic DNA Laboratory, Argentinean Forensic Anthropology Team (EAAF) Independencia 644,3A, 5000 Cordoba, Argentina.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Salado Puerto
authors.affiliation: Forensic DNA Laboratory, Argentinean Forensic Anthropology Team (EAAF) Independencia 644,3A, 5000 Cordoba, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Catelli
authors.affiliation: Forensic Genetics Unit, Institute of Legal Medicine, Faculty of Medicine, University of Santiago de Compostela, ES-15705 Santiago de Compostela, Galicia, Spain.
authors.forename: Christopher
authors.initials: C
authors.lastname: Phillips
authors.affiliation: Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal.
authors.forename: Rui
authors.initials: R
authors.lastname: Pereira
authors.affiliation: Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal; DNA Diagnostic Laboratory (LDD), State University of Rio de Janeiro (UERJ), Rio de Janeiro, Brazil.
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
authors.affiliation: Forensic DNA Laboratory, Argentinean Forensic Anthropology Team (EAAF) Independencia 644,3A, 5000 Cordoba, Argentina. Electronic address: cvullo@fibercorp.com.ar.
authors.forename: Carlos
authors.initials: C
authors.lastname: Vullo
mesh_terms: D001842:Bone and Bones / Q000737:chemistry*; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification*; D053803:Forensic Genetics / Q000379:methods* / Q000458:organization & administration*; D005787:Gene Frequency / Q000235:genetics; D005819:Genetic Markers; D005828:Genetics, Population; D060005:Genotyping Techniques; D006801:Humans; D054643:INDEL Mutation; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D044469:Racial Groups / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D004247:DNA
keywords: Ancestry informative markers (AIMs); Genetic ancestry; HGDP-CEPH; Indels; Skeletal remains