title: Hybrid bioartificial liver support in cynomolgus monkeys with D-galactosamine-induced acute liver failure.
abstract: OBJECTIVE
  To evaluate a hybrid bioartificial liver support system (HBALSS) in cynomolgus monkeys with acute liver failure.
  
  
  METHODS
  To establish a model of acute liver failure, 0.3 g/kg of D-galactosamine was injected intravenously into cynomolgus monkeys. Chinese human liver cells were introduced into a perfusion bioreactor to carry out hybrid bioartificial liver support treatment. Forty-eight hours after the injection, one group of cynomolgus monkeys received HBALSS care, and a second experimental group received no treatment. Clinical manifestations of all animals, survival time, liver and kidney functions and serum biochemistry changes were recorded. Simultaneous detection of the number, viability and function of hepatocytes in the hybrid bioartificial liver were also performed.
  
  
  RESULTS
  Forty-eight hours after the injection of D-galactosamine, serum biochemistry levels were significantly increased, whereas albumin levels and the Fischer index were significantly reduced compared to baseline (all Ps < 0.05). Of the ten monkeys in the HBALSS treatment group, five survived, with an average duration of survival of 128 ± 3 h. All cynomolgus monkeys in the control group died, with a duration of survival of 112 ± 2 h. Survival time was significantly longer with HBALSS treatment (P < 0.05). Moreover, the number, viability and function of hepatocytes were maintained at a high level with HBALSS.
  
  
  CONCLUSIONS
  The novel hybrid bioartificial liver plays a significant role in liver support by significantly reducing serum biochemistry levels and extending animal survival time.
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Yi-Chao
authors.initials: YC
authors.lastname: Zhao
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Guo-Deng
authors.initials: GD
authors.lastname: Jian
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Pan
authors.affiliation: Zhi Zhang, Yuan Cheng, Ming-Xin Pan, Yi Gao, Second Department of Hepatobiliary, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D019149:Bioreactors; D002460:Cell Line; D002470:Cell Survival; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D004195:Disease Models, Animal; D004867:Equipment Design; D005240:Feasibility Studies; D005688:Galactosamine; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D017114:Liver Failure, Acute / Q000139:chemically induced / Q000175:diagnosis / Q000378:metabolism / Q000628:therapy*; D008111:Liver Function Tests; D019164:Liver, Artificial; D008252:Macaca fascicularis; D008297:Male; D013997:Time Factors
chemical_list: D015415:Biomarkers; D005688:Galactosamine
keywords: Acute liver failure; Chinese human liver cells; Cynomolgus monkey; Human liver cell; Hybrid bioartificial liver