title: Phagocytic function of lower spleen pole and autogenous splenic implants in rats.
abstract: OBJECTIVE
  To investigate whether there are differences between the phagocytic function of the remaining lower spleen pole after subtotal splenectomy and autogenous splenic implants.
  
  
  METHODS
  Thirty-six male Wistar rats, weighting 364 ± 60g were used. They were subjected to subtotal splenectomy preserving the lower spleen pole and to autogenous splenic implant in the greater omentum. Its viability was assessed microscopically. Phagocytic function was assessed by splenic uptake of the radioisotope-labeled colloid and by macrophages counting.
  
  
  RESULTS
  The viability of the autogenous splenic implant and of the lower spleen pole was found in 33 animals, with no difference between them. The weight of the implants was higher than the lower pole of animals from groups G1, G7, G30, G60 and G120. The implants phagocytic function by radioisotope uptake was higher than the lower pole in G7 and G120 groups and it did not differ from the other groups. The number of macrophages was higher in G1, G60, G90 and G120 and did not differ from the other groups.
  
  
  CONCLUSIONS
  Until the 16th week, the phagocytic function was more pronounced in autogenous splenic implants when compared with the lower spleen pole, but it became similar thereafter.
authors.affiliation: Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Andréa Saade Daher
authors.initials: AS
authors.lastname: Borjaili
authors.affiliation: College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
authors.forename: Brunela Silva
authors.initials: BS
authors.lastname: Cerqueira
authors.affiliation: College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
authors.forename: Julio Roitman
authors.initials: JR
authors.lastname: Silbermann
authors.affiliation: College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
authors.forename: Caroline Ferraz
authors.initials: CF
authors.lastname: Rezende
authors.affiliation: College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
authors.forename: David Domingos
authors.initials: DD
authors.lastname: Freire Júnior
authors.affiliation: Nossa Senhora da Gloria Children Hospital, Vitoria, ES, Brazil.
authors.forename: Jane Sant'Ana
authors.initials: JS
authors.lastname: Castello
authors.affiliation: Vila Velha University, Vila Velha, ES, Brazil.
authors.forename: Dominik
authors.initials: D
authors.lastname: Lenz
authors.affiliation: Department of Surgery, College of Health Sciences, EMESCAM, Vitoria, ES, Brazil.
authors.forename: Danilo Nagib Salomão
authors.initials: DN
authors.lastname: Paulo
authors.affiliation: Department of Surgery, Medical School, UFMG, Belo Horizonte, MG, Brazil.
authors.forename: Tarcizo Afonso
authors.initials: TA
authors.lastname: Nunes
mesh_terms: D000818:Animals; D064592:Autografts / Q000033:anatomy & histology / Q000502:physiology*; D002452:Cell Count / Q000379:methods; D005500:Follow-Up Studies; D008264:Macrophages / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009852:Omentum; D010587:Phagocytosis / Q000502:physiology*; D011184:Postoperative Period; D017208:Rats, Wistar; D013154:Spleen / Q000033:anatomy & histology / Q000502:physiology* / Q000601:surgery / Q000637:transplantation; D013156:Splenectomy