title: Expression of the chondroitin sulphate proteoglycan, NG2, in paediatric brain tumors.
abstract: OBJECTIVE
  While neuron-glia 2 (NG2) is well-characterized in the developing brain and in adult high-grade gliomas, little is known about NG2 expression in paediatric brain tumors. Here, NG2 expression was examined in a range of paediatric brain tumors.
  
  
  METHODS
  A retrospective immunohistopathological analysis of 57 paediatric brain tumor biopsies of various tumor types was carried out. Paediatric cell lines, including two medulloblastomas and one dysembryoplastic neuroepithelial tumor, in addition to one adult high-grade glioma, were also assessed for NG2 expression.
  
  
  RESULTS
  NG2-positive staining was seen in all dysembryoplastic neuroepithelial tumors (DNETs) examined; however, only two of the fourteen medulloblastomas examined were NG2-positive. Compared to adult glioma, there was a lack of NG2 staining in the vasculature of paediatric brain tumors.
  
  
  CONCLUSIONS
  NG2 expression in paediatric brain tumors differs depending upon type and, unlike adult glioma, includes expression on lower-grade tumors.
authors.affiliation: Cellular and Molecular Neuro-oncology Group, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, U.K. samantha.higgins@port.ac.uk.
authors.forename: Samantha C
authors.initials: SC
authors.lastname: Higgins
authors.affiliation: Experimental Neuro-oncology Group, Department of Neuropathology, Institute of Psychiatry, King's College London, London, U.K.
authors.forename: Anna J
authors.initials: AJ
authors.lastname: Bolteus
authors.affiliation: Division of Neurosurgery, The Hospital for Sick Kids, Toronto, ON, Canada.
authors.forename: Laura K
authors.initials: LK
authors.lastname: Donovan
authors.affiliation: King's College Hospital, London, U.K.
authors.forename: Harutomo
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: King's College Hospital, London, U.K.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Doey
authors.affiliation: King's College Hospital, London, U.K.
authors.forename: Safa
authors.initials: S
authors.lastname: Al Sarraj
authors.affiliation: King's College Hospital, London, U.K.
authors.forename: Andrew
authors.initials: A
authors.lastname: King
authors.affiliation: King's College Hospital, London, U.K.
authors.forename: Keyoumars
authors.initials: K
authors.lastname: Ashkan
authors.affiliation: Neuropathology, Department of Medicine, Charing Cross Hospital, Imperial College, London, U.K.
authors.forename: Federico
authors.initials: F
authors.lastname: Roncaroli
authors.affiliation: Cellular and Molecular Neuro-oncology Group, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, U.K.
authors.forename: Helen L
authors.initials: HL
authors.lastname: Fillmore
authors.affiliation: Cellular and Molecular Neuro-oncology Group, Institute of Biomedical and Biomolecular Sciences, School of Pharmacy and Biomedical Sciences, University of Portsmouth, Portsmouth, U.K.
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Pilkington
mesh_terms: D000293:Adolescent; D000941:Antigens / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D060787:Neoplasm Grading; D011509:Proteoglycans / Q000378:metabolism*; D012189:Retrospective Studies; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D011509:Proteoglycans; D000099216:Chondroitin Sulfate Proteoglycan 4
keywords: Brain tumors; NG2; paediatric