title: Validation and comparison of two commercial ELISA kits and three in-house developed real-time PCR assays for the detection of potentially allergenic mustard in food.
abstract: The study compares the applicability of two commercial mustard ELISA kits (Mustard ELISA Kit-specific and Mustard ELISA Kit-total) and three in-house developed real-time PCR assays (singleplex assay for white mustard, singleplex assay for black/brown mustard and duplex assay for the detection of white, black and brown mustard). Analyses of raw and brewed model sausages containing white and black/brown mustard in the range from 1 to 50 ppm indicate that both ELISAs and the three real-time PCR assays allow the detection of traces of mustard in raw and in brewed sausages. The ELISAs were found to be more sensitive than the real-time PCR assays. When the ELISAs and real-time PCR assays were applied to the analysis of 15 commercial foodstuffs differing in their labelling concerning mustard, in one sample mustard was detected with both ELISAs and the three real-time PCR assays although mustard was not indicated on the food ingredient list.
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria; AGES, Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria.
authors.forename: Monika
authors.initials: M
authors.lastname: Palle-Reisch
authors.affiliation: AGES, Austrian Agency for Health and Food Safety, Institute for Food Safety, Department of Molecular Biology and Microbiology, Spargelfeldstraße 191, 1220 Vienna, Austria.
authors.forename: Rupert
authors.initials: R
authors.lastname: Hochegger
authors.affiliation: SEDIUM RD, Ltd., Plemenarsky Podnik 29, 530 03 Pardubice-Nemosice, Czech Republic.
authors.forename: Stepan
authors.initials: S
authors.lastname: Štumr
authors.affiliation: SEDIUM RD, Ltd., Plemenarsky Podnik 29, 530 03 Pardubice-Nemosice, Czech Republic.
authors.forename: Kveta
authors.initials: K
authors.lastname: Korycanova
authors.affiliation: Department of Analytical Chemistry, University of Vienna, Währinger Straße 38, 1090 Vienna, Austria. Electronic address: margit.cichna@univie.ac.at.
authors.forename: Margit
authors.initials: M
authors.lastname: Cichna-Markl
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D008461:Meat Products / Q000032:analysis*; D009149:Mustard Plant / Q000737:chemistry* / Q000235:genetics; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D031226:Sinapis / Q000737:chemistry* / Q000235:genetics
chemical_list: D000485:Allergens; D010940:Plant Proteins
keywords: ELISA; Food allergen; Mustard; Real-time PCR; Validation