title: Derivation of cell-adapted Sacbrood virus (SBV) from the native Korean honeybee.
abstract: Sacbrood virus (SBV), a causative agent of larval death in honeybees, is one of the most devastating diseases in bee industry throughout the world. Lately the Korean Sacbrood virus (KSBV) induced great losses in Korean honeybee (Apis cerana) colonies. However, there is no culture system available for honeybee viruses, including SBV, therefore, the research on honeybee viruses is practically limited until present. In this study, we investigated the growth and replication of SBV in cell cultures. The replication signs of KSBV after passages from mammalian cells was identified and confirmed by using combined approaches with nested, quantitative, negative-strand PCR and electron microscopy along with in vivo experiment. The results revealed that mammalian cell lines, including Vero cells could support the replication KSBV. Although there were no signs of cytopathic effect (CPE) in cells, it was for the first time demonstrated that SBV could be replicated in cells through the sequential passages linked with cell adaptation. KSBV from the present study would be a valuable source to understand the mechanism of pathogenicity of sacbrood virus in the future.
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Chang-Hee
authors.initials: CH
authors.lastname: Kweon
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Yoo
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Jin-Hyeong
authors.initials: JH
authors.lastname: Noh
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Kondreddy Eswar
authors.initials: KE
authors.lastname: Reddy
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Dong-Kun
authors.initials: DK
authors.lastname: Yang
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Cha
authors.affiliation: Animal and Plant Quarantine Agency, 480 Anyang-ro, Manan-gu, Anyang-si, Gyeonggi-do, Republic of Korea. Electronic address: kangsw777@korea.kr.
authors.forename: Seung-Won
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000220:Adaptation, Biological; D000818:Animals; D001516:Bees / Q000821:virology*; D002460:Cell Line; D007723:Korea; D008969:Molecular Sequence Data; D012328:RNA Viruses / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D012692:Serial Passage; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D012367:RNA, Viral
keywords: Apis cerana; Cell cultures; Korean honeybee; Replication; SBV; Sacbrood virus