title: Optimisation of isolation procedure for pyrrolizidine alkaloids from Rindera umbellata Bunge.
abstract: Procedure for isolation of pyrrolizidine alkaloids (PAs) from Rindera umbellata Bunge plant species was optimised. Different extraction media (methanol, ethanol and sulphuric acid), concentration and volume of sulphuric acid, pH of PA solution for alkaline extraction, extraction time and techniques (maceration, ultrasonic and overhead rotary mixer assisted extraction) were investigated. The yields of six PAs (7-angeloyl heliotridane, 7-angeloyl heliotridine, lindelofine, 7-angeloyl rinderine, punctanecine and heliosupine) were monitored by GC-MS/FID. The best results for the isolation all of six PAs were obtained when the extraction was performed with 1 M sulphuric acid (30 mL per 1.00 g of dried sample) by overhead rotary mixer during three days. Optimal pH value for alkaline extraction of PAs with CH₂Cl₂ was 9, and the extraction should be performed with four portions of 30 mL of CH₂Cl₂. This procedure could be also useful for a plant sample preparation for GC and LC analyses of PAs.
authors.affiliation: a Faculty of Chemistry, University of Belgrade , Studentski Trg 16, Belgrade 11158 , Serbia.
authors.forename: Boris M
authors.initials: BM
authors.lastname: Mandić
authors.forename: Marina D
authors.initials: MD
authors.lastname: Vlajić
authors.forename: Snežana S
authors.initials: SS
authors.lastname: Trifunović
authors.forename: Milena R
authors.initials: MR
authors.lastname: Simić
authors.forename: Ljubodrag V
authors.initials: LV
authors.lastname: Vujisić
authors.forename: Ivan M
authors.initials: IM
authors.lastname: VuČković
authors.forename: Miroslav M
authors.initials: MM
authors.lastname: Novaković
authors.forename: Snežana D
authors.initials: SD
authors.lastname: Nikolić-Mandić
authors.forename: Vele V
authors.initials: VV
authors.lastname: Tešević
authors.forename: Vlatka V
authors.initials: VV
authors.lastname: Vajs
authors.forename: Slobodan M
authors.initials: SM
authors.lastname: Milosavljević
mesh_terms: D028082:Boraginaceae / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D011763:Pyrrolizidine Alkaloids / Q000302:isolation & purification*; D012997:Solvents
chemical_list: D011763:Pyrrolizidine Alkaloids; D012997:Solvents; C014795:retronecine; C518047:heliotridane; C018336:indicine
keywords: GC–MS; Rindera umellata Bunge; isolation; pyrrolizidine alkaloids