title: Genotypes and antibiotic resistance of bovine Campylobacter and their contribution to human campylobacteriosis.
abstract: Campylobacter jejuni and Campylobacter coli are the most important bacterial causes of human gastroenteritis. Chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. However, there is a paucity of information available regarding Campylobacter in Swiss cattle and their role for human campylobacteriosis. To gain more information on genotypes and antibiotic resistance of bovine C. jejuni and C. coli and on their contribution to human disease, 97 cattle isolates were analysed. Multilocus sequence typing (MLST) and flaB typing were applied and the gyrA and 23S rRNA genes were screened for point mutations responsible for quinolone and macrolide resistance, respectively. A total of 37 sequence types (STs) and 44 flaB types were identified, including two sequence types and five flaB types not previously described. Most common sequence types were ST21 (21%), ST61 (12%) and ST48 (11%). Only one isolate was macrolide resistant while 31% (n = 30) were quinolone resistant. Source attribution indicated chicken as the main source of human infection with cattle being second. In conclusion, cattle should not be underestimated as a potential source of human campylobacteriosis.
authors.affiliation: Institute of Veterinary Bacteriology,Vetsuisse Faculty,University of Bern,Bern,Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Jonas
authors.affiliation: Institute of Veterinary Bacteriology,Vetsuisse Faculty,University of Bern,Bern,Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Kittl
authors.affiliation: Institute of Veterinary Bacteriology,Vetsuisse Faculty,University of Bern,Bern,Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Overesch
authors.affiliation: Institute of Veterinary Bacteriology,Vetsuisse Faculty,University of Bern,Bern,Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Kuhnert
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002169:Campylobacter Infections / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D016123:Campylobacter jejuni / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000635:transmission; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D005759:Gastroenteritis / Q000382:microbiology; D005838:Genotype; D006801:Humans; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D015363:Quinolones / Q000494:pharmacology; D012338:RNA, Ribosomal, 23S / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D015363:Quinolones; D012338:RNA, Ribosomal, 23S; D005408:Flagellin; C416532:flaB flagellin; D027081:DNA Gyrase
keywords: Antibiotic resistance; Campylobacter coli; Campylobacter jejuni; MLST; flaB; gastroenteritis; genoptyping; source attribution; zoonosis