title: Epigenetically silenced long noncoding-SRHC promotes proliferation of hepatocellular carcinoma.
abstract: OBJECTIVE
  To investigate the expression of SRHC and the role of SRHC in the pathogenesis of hepatocellular carcinoma (HCC).
  
  
  METHODS
  We analyzed HCC samples and matched non-tumor liver tissues (controls) collected from 81 patients who underwent hepatectomy in Shanghai, China. The expression levels of SRHC were determined by quantitative reverse-transcription polymerase chain reaction. Statistical analyses were used to associate the levels of SRHC with tumor features and patient outcomes.
  
  
  RESULTS
  We found that a lower SRHC expression level was significantly more frequent in tissues with a high serum a-fetoprotein level (positive, >20 µg/L, P = 0.004) and a low degree of differentiated tumors (poorly differentiated, P = 0.017). Furthermore, we found that the promoter region of SRHC contains a CpG-rich island and that SRHC is down-regulated in tumors by DNA methylation.
  
  
  CONCLUSIONS
  Here, we identified a new long noncoding RNA designated as SRHC that is capable of inhibiting cancer proliferation and is down-regulated in tumors at least partly by DNA methylation.
authors.affiliation: The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, 225 Changhai Road, Shanghai, 200438, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zheng
authors.forename: Sen
authors.initials: S
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.forename: Sheng-Xian
authors.initials: SX
authors.lastname: Yuan
authors.forename: Fu-Quan
authors.initials: FQ
authors.lastname: Wu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Yan
authors.forename: Shu-Han
authors.initials: SH
authors.lastname: Sun
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Zhou
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D062085:RNA, Long Noncoding