title: Toll-like receptors recognize distinct proteinase-resistant glycoconjugates in Campylobacter jejuni and Escherichia coli.
abstract: Campylobacter jejuni causes gastroenteritis and autoimmune neuropathy Guillain-Barré syndrome. The mechanism by which C. jejuni infection results in such the hyperimmunity is not completely understood. Host immunity plays an important role in the disease pathogenesis; however, little is known how immune system recognizes this human pathogen. In this study, we report that Toll-like receptors recognize distinct proteinase K-resistant glycoconjugates in C. jejuni and Escherichia coli. Lipopolysaccharide is solely proteinase-resistant glycoconjugate in E. coli. In contrast, C. jejuni possesses at least five different components that are resistant to proteinase digestion and are capable of inducing NF-κB activation through TLR2 and TLR4. Possession of multiple activators of Toll-like receptors may be the unique strategy of C. jejuni to trigger hyperimmunity.
authors.affiliation: Division of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: phongsisay@gmail.com.
authors.forename: Vongsavanh
authors.initials: V
authors.lastname: Phongsisay
authors.affiliation: Division of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Hara
authors.affiliation: Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Fujimoto
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis; D019286:Endopeptidase K / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D006001:Glycoconjugates / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: D016207:Cytokines; D006001:Glycoconjugates; D016328:NF-kappa B; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D019286:Endopeptidase K
keywords: Campylobacter jejuni; Inflammation; Toll-like receptors