title: [Genotyping and evaluation of infection dynamics in a Colombian isolate of Leptospira santarosai in hamster as an experimental model].
abstract: BACKGROUND
  Is necessary to develop models for the study of leptospirosis.
  
  
  OBJECTIVE
  To genotype a Colombian strain of Leptospira isolated from a human with Weil´s syndrome and to evaluate its infection dynamics in the hamster experimental model.
  
  
  METHODS
  Genotyping was performed by amplification and sequence analysis of the rrs 16S and lipL32 genes. The median lethal dose was determined in intraperitoneally inoculated hamsters. The patterns of clinical chemistry, the duration of leptospiremia, leptospiruria and pathological findings were studied and compared in the same animal model infected with L. interrogans (Fiocruz L1-130).
  
  
  RESULTS
  Molecular typing revealed that the isolate corresponded to the pathogenic species L. santarosai, which was recovered from hamsters´ kidneys and lungs and detected by lipL32 PCR from day 3 post-infection in these organs. There was a marked increase of C-reactive protein in animals at day 5 post-infection (3.25 mg/dl; normal value: 0.3 mg/dl) with decreases by day 18 (2.60 mg/dl: normal value: 0.8 mg/dl). Biomarkers of urea showed changes consistent with possible renal acute failure (day 5 post-infection: 49.01 mg/dl and day 18 post-infection: 53.71 mg/dl). Histopathological changes included interstitial pneumonia with varying degrees of hemorrhage and interstitial nephritis.
  
  
  CONCLUSIONS
  The pathogenic species L. santarosai was identified in Colombia. Its pathogenicity as determined by tropism to lung and kidney was comparable to that of L. interrogans Fiocruz L1-130, well known for its virulence and pulmonar tropism. The biological aspects studied here had never before been evaluated in an autochthonous isolate.
authors.affiliation: Facultad de Medicina, Universidad CES, Medellín, Colombia.
authors.forename: Piedad
authors.initials: P
authors.lastname: Agudelo-Flórez
authors.affiliation: Facultad de Medicina, Universidad CES, Medellín, Colombia.
authors.forename: Harold
authors.initials: H
authors.lastname: Durango
authors.affiliation: Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Diego
authors.initials: D
authors.lastname: Aranzazu
authors.affiliation: Escuela de Medicina Veterinaria, Facultad de Ciencias Agrarias, Universidad de Antioquia, Medellín, Colombia.
authors.forename: Juan David
authors.initials: JD
authors.lastname: Rodas
authors.affiliation: University of Texas Medical Branch, Galveston, Texas, USA.
authors.forename: Bruno
authors.initials: B
authors.lastname: Travi
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D003105:Colombia; D006224:Cricetinae; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005260:Female; D005838:Genotype; D054884:Host-Pathogen Interactions; D006801:Humans; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D007919:Leptospira / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D007921:Leptospira interrogans / Q000235:genetics / Q000472:pathogenicity; D007922:Leptospirosis / Q000382:microbiology*; D007928:Lethal Dose 50; D008074:Lipoproteins / Q000235:genetics; D008168:Lung / Q000382:microbiology / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000382:microbiology; D008297:Male; D008647:Mesocricetus / Q000382:microbiology*; D023421:Models, Animal; D009395:Nephritis, Interstitial / Q000382:microbiology; D009928:Organ Specificity; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C406589:LipL32 protein, Leptospira; D008074:Lipoproteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S