title: Ovarian failure in diabetic rat model: nuclear factor-kappaB, oxidative stress, and pentraxin-3.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effects of diabetes mellitus (DM) on ovarian reserve and injury by considering laboratory and histopathological parameters in rat models.
  
  
  METHODS
  An experimental DM model was created in 16 rats. Eight rats with normal blood glucose levels were included in the control group. Diabetic rats were divided randomly into two groups: nontreated and resveratrol-treated groups. Histopathological examination and nuclear factor (NF)-κB immunoexpression level determination were performed. Plasma malondialdehyde, glutathione, pentraxin-3, and anti-Müllerian hormone levels were measured. Relations between the variables were compared by Student t test, analysis of variance, and Mann-Whitney U and χ(2) tests.
  
  
  RESULTS
  We found statistically significantly lower glutathione and anti-Müllerian hormone levels, and higher malondialdehyde and pentraxin-3 levels in nontreated diabetic group when compared with the control and resveratrol-treated diabetic groups. Stromal degeneration, follicle degeneration, stromal fibrosis scores, and NF-κB immunoexpression levels were significantly higher in nontreated diabetic rats. Primordial and primary follicle counts were significantly lower in the nontreated diabetic group when compared with the control and resveratrol-treated groups. There was no statistically significant difference in secondary and tertiary follicles between these groups.
  
  
  CONCLUSIONS
  These findings provide strong evidence that the ovarian follicle pool in nontreated diabetic rats is affected in the early stages of the follicle development process. We precluded negative effects of DM on ovaries by inhibiting the NF-κB pathway with resveratrol. We thought that the NF-κB pathway plays a role in the pathophysiology of ovarian failure in diabetic rats. Further studies should evaluate this precise mechanism that leads to a decline in the anti-Müllerian hormone levels. In addition, the relationship between this abnormality and reproductive function in diabetic patients should be analyzed further.
authors.affiliation: Physiology Department, School of Medicine, Gaziosmanpasa University, Tokat, Turkey.
authors.forename: Oytun
authors.initials: O
authors.lastname: Erbas
authors.affiliation: Obstetrics and Gynecology-Perinatology Department, Celal Bayar University, Manisa, Turkey. Electronic address: gursoypala@yahoo.com.
authors.forename: Halil Gursoy
authors.initials: HG
authors.lastname: Pala
authors.affiliation: Pathology Department, Izmir Tepecik Training and Research Hospital, Izmir, Turkey.
authors.forename: Emel Ebru
authors.initials: EE
authors.lastname: Pala
authors.affiliation: Histology and Embryology Department, School of Medicine, Ege University, Izmir, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Oltulu
authors.affiliation: Histology and Embryology Department, School of Medicine, Ege University, Izmir, Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Aktug
authors.affiliation: Histology and Embryology Department, School of Medicine, Ege University, Izmir, Turkey.
authors.forename: Altug
authors.initials: A
authors.lastname: Yavasoglu
authors.affiliation: Physiology Department, School of Medicine, Ege University, Izmir, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Taskiran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D002097:C-Reactive Protein / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D065851:Ovarian Reserve; D010053:Ovary / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D016649:Primary Ovarian Insufficiency / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D000077185:Resveratrol; D000683:Serum Amyloid P-Component / Q000378:metabolism*; D013267:Stilbenes / Q000627:therapeutic use; D000099153:Pentraxins
chemical_list: D000975:Antioxidants; D002097:C-Reactive Protein; D016328:NF-kappa B; D000077185:Resveratrol; D000683:Serum Amyloid P-Component; D013267:Stilbenes; D000099153:Pentraxins
keywords: NF-κB; diabetes mellitus; pentraxin-3; premature ovarian failure; resveratrol