title: Population genetics for 23 Y-STR loci in Tibetan in China and confirmation of DYS448 null allele.
abstract: Tibetan is one of 56 ethnic groups in China, where a level of genetic sub-structure might be expected. Although a global analysis of Y-chromosomal haplotype diversity for 23 STR loci and Y-STR databases with PPY23 kit were created with collaborative effort, there was a lack of data for Tibetan population. In this study we evaluated 248 unrelated male individuals of Chinese Tibetan living in the Tibet Autonomous Region to explore the underlying genetic structure of Tibetan populations. These samples were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) by using PPY23 kit. A total of 224 different haplotypes were found. Haplotype diversity was 0.9990. Both Rst pairwise analyses and multidimensional scaling plot showed the genetic structure of Tibetan population was significantly different from some of Chinese ethnic groups and neighboring populations. There were few interesting null features at DYS448 observed by PPY23 that deserved some comment. It revealed that PPY23 marker set provided substantially stronger discriminatory power in Tibetan population.
authors.affiliation: Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China; Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ye
authors.affiliation: The Center for Material Evidence, Tibet Autonomous Region Public Security, Lhasa 850000, Tibet Autonomous Region, China.
authors.forename: Jingshang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Fan
authors.affiliation: Department of Forensic Analytical Toxicology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China.
authors.forename: Linchuan
authors.initials: L
authors.lastname: Liao
authors.affiliation: Department of Forensic Biology, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, China. Electronic address: prof.hou@gmail.com.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hou
mesh_terms: D044466:Asian People / Q000235:genetics*; D002681:China; D004247:DNA / Q000032:analysis / Q000097:blood / Q000235:genetics; D030541:Databases, Genetic; D005787:Gene Frequency; D050173:Genes, Y-Linked / Q000235:genetics*; D005828:Genetics, Population; D006239:Haplotypes; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D018609:Tibet / Q000208:ethnology
chemical_list: D004247:DNA
keywords: DYS448; PPY23; Tibetan; Y-STR