title: Expression of PRX36, PMEI6 and SBT1.7 is controlled by complex transcription factor regulatory networks for proper seed coat mucilage extrusion.
abstract: Mucilage secretory cells (MSC) form an intriguing cell layer important for seed germination. In Arabidopsis thaliana, several master transcription factors (TFs) and "actor" proteins have already been identified as key players for seed coat differentiation including epidermal cell formation, mucilage production and extrusion. The regulation of the genes coding for MSC cell wall "actor" proteins by TFs needs to be better established. Here, the expression and the regulation of 3 known actors (PRX36, PMEI6, SBT1.7) and 2 additional putative actors (PRX56, DIR12) have been analyzed in T-DNA mutants affected in master TFs (ap2, egl3/gl3, gl2, myb5, tt8, ttg1, ttg2 and luh1/mum1). Genes with somehow similar function are differentially regulated and conversely, genes with different functions are regulated in similar manner.
authors.affiliation: a Université de Toulouse; UPS; UMR 5546; Laboratoire de Recherche en Sciences Végétales ; Castanet-Tolosan , France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Ranocha
authors.forename: Edith
authors.initials: E
authors.lastname: Francoz
authors.forename: Vincent
authors.initials: V
authors.lastname: Burlat
authors.forename: Christophe
authors.initials: C
authors.lastname: Dunand
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D062087:Plant Mucilage / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013381:Subtilisins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D062087:Plant Mucilage; D014157:Transcription Factors; C583987:SBT1.7 protein, Arabidopsis; D013381:Subtilisins
keywords: AP2, APETALA2; Arabidopsis; EGL3, ENHANCER OF GLABRA3; GL2, GLABRA2; LUH/MUM1, LEUNIG HOMOLOG1/ MODIFIED MUCILAGE1; MSC, mucilage secretory cells; TT8, TRANSPARENT TESTA8; TTG1, TRANSPARENT TESTA GLABRA1; TTG2, TRANSPARENT TESTA GLABRA2; cell wall; class III peroxidases; pectic mucilage; seed coat; transcription factors