title: Enhanced seed oil content by overexpressing genes related to triacylglyceride synthesis.
abstract: Oilseed rape (Brassica napus) is one of the most important oilseed crops globally. To meet increasing demand for oil-based products, the ability to enhance desirable oil content in the seed is required. This study assessed the capability of five genes in the triacylglyceride (TAG) synthesis pathway to enhance oil content. The genes BnGPDH, BnGPAT, BnDGAT, ScGPDH and ScLPAAT were overexpressed separately in a tobacco (Nicotiana benthamiana) model system, and simultaneously by pyramiding in B. napus, under the control of a seed specific Napin promoter. ScLPAAT transgenic plants showed a significant increase of 6.84% to 8.55% in oil content in tobacco seeds, while a ~4% increase was noted for BnGPDH and BnGPAT transgenic seeds. Seed-specific overexpression of all four genes in B. napus resulted in as high a 12.57% to 14.46% increased in seed oil content when compared to WT, equaling close to the sum of the single-gene overexpression increases in tobacco. Taken together, our study demonstrates that BnGPDH, BnGPAT and ScLPAAT may effectively increase seed oil content, and that simultaneous overexpression of these in transgenic B. napus may further enhance the desirable oil content relative to single-gene overexpressors.
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: jutus.et.in.cute@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: xiayuping1984@163.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: wulei686@sina.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.affiliation: The Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Agronomy College, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address: fudhui@163.com.
authors.forename: Donghui
authors.initials: D
authors.lastname: Fu
authors.affiliation: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland 4072, Australia. Electronic address: a.hayward@uq.edu.au.
authors.forename: Alice
authors.initials: A
authors.lastname: Hayward
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: alaluo@163.com.
authors.forename: Junling
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: yanxiaohong@caas.cn.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Yan
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: xiongxj1974@163.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: z7713ster@gmail.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Fu
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: wugang@caas.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Key Laboratory of Oil Crop Biology of the Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, No. 2 Xudong 2nd Road, Wuhan 430062, China. Electronic address: cmlu@oilcrops.cn.
authors.forename: Changming
authors.initials: C
authors.lastname: Lu
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D029688:Brassica napus / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D017343:Genes, Plant; D010938:Plant Oils / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D012639:Seeds / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D010938:Plant Oils; D010940:Plant Proteins; D014280:Triglycerides
keywords: Brassica napus; DGAT; GPAT; GPDH; LPAAT; Tobacco