title: [Effect of smoking on the microRNAs expression in pneumoconiosis patients].
abstract: OBJECTIVE
  To investigate the effect of smoking on the microRNAs (miRNAs) expression in pneumoconiosis patients.
  
  
  METHODS
  Real-time qPCR was used to measure the expression levels of miR-21, miR-200c, miR-16, miR-204, miR-206, miR-155, let-7g, miR-30b, and miR-192 in 36 non-smoking patients with pneumoconiosis and 38 smoking patients with pneumoconiosis, and the differences in expression levels between the two groups were evaluated by two-independent samples t-test.
  
  
  RESULTS
  The expression of miR-192 in serum showed a significant difference between non-smoking and smoking pneumoconiosis patients (P < 0.05), and it decreased gradually in smoking patients with stage I and II pneumoconiosis. In the serum of all pneumoconiosis patients, the expression level of miR-16 was the highest, while the expression level of miR-204 was the lowest.
  
  
  CONCLUSIONS
  Pneumoconiosis patients have differential expression of miRNAs in serum, and smoking has an effect on the miRNAs expression in pneumoconiosis patients.
authors.affiliation: Tianjin Centers for Disears Control and Prevention, Tianjin 300011, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: E-mail: wangyanrang@126.com.
authors.forename: Yanrang
authors.initials: Y
authors.lastname: Wang
authors.forename: Deyi
authors.initials: D
authors.lastname: Yang
authors.forename: Yitao
authors.initials: Y
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jundi
authors.initials: J
authors.lastname: Xia
authors.forename: Louxin
authors.initials: L
authors.lastname: Zhang
authors.forename: Lianhong
authors.initials: L
authors.lastname: Xie
mesh_terms: D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D011009:Pneumoconiosis / Q000378:metabolism / Q000503:physiopathology*; D016133:Polymerase Chain Reaction; D012907:Smoking / Q000009:adverse effects*
chemical_list: D035683:MicroRNAs