title: Protective effect of magnesium and selenium on cadmium toxicity in the isolated perfused rat liver system.
abstract: The isolated perfused rat liver (IPRL) model has been used into toxicology study of rat liver. This model provides an opportunity at evaluation of liver function in an isolated setting. Studies showed that Cd, in a dose-dependent manner, induced toxic effects in IPRL models, and these effects were associated with aminotransferase activity and lipid peroxidation. The aim of this study was to investigate whether Mg  and/or Se could have protective effects against the Cd toxicity in the IPRL model. Male Wistar rats (9-10 weeks) weighing 260-300 gr were used in this study. They were randomly divided into 8 groups of 4-6 rats per cage. In group 1, liver was perfused by Krebs-Henseleit buffer without MgSO4 (Control). Groups 2-8 were exposed to Mg, Se, Cd, Mg +Se, Cd + Mg, Cd + Se, Cd + Mg + Se respectively in Krebs-Henseleit buffer with no added MgSo4. Biochemical changes in the liver were examined within 90 minutes, and the result showed that the exposure to Cd, lowered glutathione level, while it increased malondialdehyde level and aminotransferase activities in IPRL model. Mg administration during exposure to Cd reduces the toxicity of Cd in the liver isolated while Se administration during exposure to Cd did not decrease Cd hepatotoxicity. Nevertheless, simultaneous treatment with Se and Mg on Cd toxicity have strengthened protective effects than the supplementation of Se alone in the liver.
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Toxicology and Pharmacology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. bahraman62@gmail.com.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghaffarian-Bahraman
authors.affiliation: Department of Toxicology, School of Veterinary Medicine, University of Semnan, Semnan, Iran. Shahroozian@gmail.com.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Shahroozian
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran. abbasj63@gmail.com.
authors.forename: Abbas
authors.initials: A
authors.lastname: Jafari
authors.affiliation: Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. ghazikha@tums.ac.ir.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Ghazi-Khansari
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D005947:Glucose; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D014325:Tromethamine
chemical_list: C074097:Krebs-Henseleit solution; D002104:Cadmium; D014325:Tromethamine; D008315:Malondialdehyde; D012643:Selenium; D008274:Magnesium; D005947:Glucose