title: Electrospun aligned poly(propylene carbonate) microfibers with chitosan nanofibers as tissue engineering scaffolds.
abstract: In this study, parallel-aligned poly(propylene carbonate) (PPC) microfibers with a fiber diameter of 1.48±0.42 μm were prepared by electrospinning and modified by oxygen plasma treatment. Next, chitosan nanofibers with a fiber diameter size of 278±98 nm were introduced into the PPC fiber mats by freeze drying. Morphological analyses showed that the PPC scaffolds treated with 0.05 mg/ml chitosan solution provided the best micro and nanofiber structure with abundant chitosan nanofibers but without the formation of films. Surface chemical properties were analyzed by X-ray photoelectron spectroscopy (XPS). The initial water contact angle of the scaffolds decreased from 122.3±0.4° for neat PPC scaffolds to 53.8±1.6° for scaffolds with plasma treatment and chitosan nanofibers. The mechanical properties of the scaffolds were affected by plasma treatment with Young's modulus experiencing a reduction of 63%. Meanwhile, Young's modulus experienced a 26% improvement after the introduction of chitosan nanofibers. Fibroblast cells were cultured on the scaffolds to study the effects of both the plasma treatment and the introduction of chitosan nanofibers on cell adhesion, proliferation, and morphology. The scaffolds with PPC microfibers and chitosan nanofibers showed a superior cell response in terms of cell attachment, cell proliferation, and cell-scaffold interactions over the other scaffolds.
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangdong, China; Wisconsin Institute for Discovery, University of Wisconsin, Madison, WI, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Jing
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangdong, China; Wisconsin Institute for Discovery, University of Wisconsin, Madison, WI, USA.
authors.forename: Hao-Yang
authors.initials: HY
authors.lastname: Mi
authors.affiliation: Wisconsin Institute for Discovery, University of Wisconsin, Madison, WI, USA; Department of Mechanical Engineering, University of Wisconsin, Madison, WI, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.affiliation: The Key Laboratory for Polymer Processing Engineering of Ministry of Education, National Engineering Research Center of Novel Equipment for Polymer Processing, South China University of Technology, Guangdong, China. Electronic address: pmxfpeng@scut.edu.cn.
authors.forename: Xiang-Fang
authors.initials: XF
authors.lastname: Peng
authors.affiliation: Wisconsin Institute for Discovery, University of Wisconsin, Madison, WI, USA; Department of Mechanical Engineering, University of Wisconsin, Madison, WI, USA. Electronic address: turng@engr.wisc.edu.
authors.forename: Lih-Sheng
authors.initials: LS
authors.lastname: Turng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003599:Cytoskeleton / Q000187:drug effects; D004560:Electricity; D005612:Freeze Drying; D057927:Hydrophobic and Hydrophilic Interactions; D055595:Mechanical Phenomena; D051379:Mice; D055616:Microtechnology; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry; D058626:Plasma Gases / Q000737:chemistry; D011407:Propane / Q000031:analogs & derivatives* / Q000737:chemistry; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D017155:Wettability
chemical_list: D001672:Biocompatible Materials; D058626:Plasma Gases; C045990:propylene carbonate; D048271:Chitosan; D010100:Oxygen; D011407:Propane
keywords: Chitosan; Electrospinning; Freeze drying; Plasma treatment; Poly(propylene carbonate) (PPC); Tissue engineering