title: Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire.
abstract: KAT6 histone acetyltransferases (HATs) are highly conserved in eukaryotes and have been shown to play important roles in transcriptional regulation. Here, we demonstrate that the Drosophila KAT6 Enok acetylates histone H3 Lys 23 (H3K23) in vitro and in vivo. Mutants lacking functional Enok exhibited defects in the localization of Oskar (Osk) to the posterior end of the oocyte, resulting in loss of germline formation and abdominal segments in the embryo. RNA sequencing (RNA-seq) analysis revealed that spire (spir) and maelstrom (mael), both required for the posterior localization of Osk in the oocyte, were down-regulated in enok mutants. Chromatin immunoprecipitation showed that Enok is localized to and acetylates H3K23 at the spir and mael genes. Furthermore, Gal4-driven expression of spir in the germline can largely rescue the defective Osk localization in enok mutant ovaries. Our results suggest that the Enok-mediated H3K23 acetylation (H3K23Ac) promotes the expression of spir, providing a specific mechanism linking oocyte polarization to histone modification.
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
authors.forename: Fu
authors.initials: F
authors.lastname: Huang
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
authors.forename: Ariel
authors.initials: A
authors.lastname: Paulson
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
authors.forename: Arnob
authors.initials: A
authors.lastname: Dutta
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Venkatesh
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA;
authors.forename: Michaela
authors.initials: M
authors.lastname: Smolle
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA jlw@stowers.org sma@stowers.org.
authors.forename: Susan M
authors.initials: SM
authors.lastname: Abmayr
authors.affiliation: Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA; jlw@stowers.org sma@stowers.org.
authors.forename: Jerry L
authors.initials: JL
authors.lastname: Workman
mesh_terms: D000107:Acetylation; D000818:Animals; D047369:Chromatin Immunoprecipitation; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology* / Q000201:enzymology* / Q000235:genetics; D004625:Embryo, Nonmammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008840:Microfilament Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D009865:Oocytes / Q000166:cytology / Q000201:enzymology; D010053:Ovary / Q000378:metabolism; D020033:Protein Isoforms
chemical_list: D029721:Drosophila Proteins; D006657:Histones; D008840:Microfilament Proteins; D020033:Protein Isoforms; C109767:mael protein, Drosophila; C091675:osk protein, Drosophila; C402385:spir protein, Drosophila; C000595040:Enok protein, Drosophila; D051548:Histone Acetyltransferases
keywords: H3K23 acetylation; Oskar localization; germ plasm