title: CCR6-dependent positioning of memory B cells is essential for their ability to mount a recall response to antigen.
abstract: Chemokine-dependent localization of specific B cell subsets within the immune microarchitecture is essential to ensure successful cognate interactions. Although cognate interactions between T cells and memory B cells (B(mem)) are essential for the secondary humoral immune responses, the chemokine response patterns of B(mem) cells are largely unknown. In contrast to naive B cells, this study shows that Ag-specific B(mem) cells have heightened expression of CCR6 and a selective chemotactic response to the CCR6 ligand, CCL20. Although CCR6 appears be nonessential for the initial clonal expansion and maintenance of B(mem), CCR6 is essential for the ability of B(mem) to respond to a recall response to their cognate Ag. This dependency was deemed intrinsic by studies in CCR6-deficient mice and in bone marrow chimeric mice where CCR6 deficiency was limited to the B cell lineage. Finally, the mis-positioning of CCR6-deficient B(mem) was revealed by immunohistological analysis with an altered distribution of CCR6-deficient B(mem) from the marginal and perifollicular to the follicular/germinal center area.
authors.affiliation: Department of Immune Regulation and Intervention, Division of Transplantation, Immunology, and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom; raul_andres.elgueta_rebolledo@kcl.ac.uk.
authors.forename: Raul
authors.initials: R
authors.lastname: Elgueta
authors.affiliation: Department of Experimental Immunobiology, Division of Transplantation, Immunology and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom;
authors.forename: Ellen
authors.initials: E
authors.lastname: Marks
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03766; Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Nowak
authors.affiliation: Department of Immune Regulation and Intervention, Division of Transplantation, Immunology, and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom;
authors.forename: Shinelle
authors.initials: S
authors.lastname: Menezes
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03766; Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Micah
authors.initials: M
authors.lastname: Benson
authors.affiliation: Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03766; Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Vanitha S
authors.initials: VS
authors.lastname: Raman
authors.affiliation: Department of Immune Regulation and Intervention, Division of Transplantation, Immunology, and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom;
authors.forename: Carla
authors.initials: C
authors.lastname: Ortiz
authors.affiliation: Department of Immune Regulation and Intervention, Division of Transplantation, Immunology, and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom;
authors.forename: Samuel
authors.initials: S
authors.lastname: O'Connell
authors.affiliation: Merck KGaA, 64293 Darmstadt, Germany.
authors.forename: Henry
authors.initials: H
authors.lastname: Hess
authors.affiliation: Department of Experimental Immunobiology, Division of Transplantation, Immunology and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom;
authors.forename: Graham M
authors.initials: GM
authors.lastname: Lord
authors.affiliation: Department of Immune Regulation and Intervention, Division of Transplantation, Immunology, and Mucosal Biology, Guy's Hospital, King's College London, London SE1 9RT, United Kingdom; Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03766; Norris Cotton Cancer Center, Dartmouth-Hitchcock Medical Center, Lebanon, NH 03756; and.
authors.forename: Randolph
authors.initials: R
authors.lastname: Noelle
mesh_terms: D064591:Allografts; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D016026:Bone Marrow Transplantation; D054418:Chemokine CCL20 / Q000235:genetics / Q000276:immunology; D002633:Chemotaxis / Q000235:genetics / Q000276:immunology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000276:immunology; D018858:Germinal Center / Q000166:cytology / Q000276:immunology; D007156:Immunologic Memory / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D054399:Receptors, CCR6 / Q000235:genetics / Q000276:immunology*; D018183:Transplantation Chimera / Q000276:immunology
chemical_list: D000941:Antigens; C493447:CCL20 protein, mouse; C498126:CCR6 protein, mouse; D054418:Chemokine CCL20; D054399:Receptors, CCR6