title: Systematic conservation planning for groundwater ecosystems using phylogenetic diversity.
abstract: Aquifer ecosystems provide a range of important services including clean drinking water. These ecosystems, which are largely inaccessible to humans, comprise a distinct invertebrate fauna (stygofauna), which is characterized by narrow distributions, high levels of endemism and cryptic species. Although being under enormous anthropogenic pressure, aquifers have rarely been included in conservation planning because of the general lack of knowledge of species diversity and distribution. Here we use molecular sequence data and phylogenetic diversity as surrogates for stygofauna diversity in aquifers of New South Wales, Australia. We demonstrate how to incorporate these data as conservation features in the systematic conservation planning software Marxan. We designated each branch of the phylogenetic tree as a conservation feature, with the branch length as a surrogate for the number of distinct characters represented by each branch. Two molecular markers (nuclear 18S ribosomal DNA and mitochondrial cytochrome oxidase subunit I) were used to evaluate how marker variability and the resulting tree topology affected the site-selection process. We found that the sites containing the deepest phylogenetic branches were deemed the most irreplaceable by Marxan. By integrating phylogenetic data, we provide a method for including taxonomically undescribed groundwater fauna in systematic conservation planning.
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Asmyhr
authors.affiliation: Australian Rivers Institute, Griffith University, Brisbane, Queensland, Australia.
authors.forename: Simon
authors.initials: S
authors.lastname: Linke
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
authors.forename: Grant
authors.initials: G
authors.lastname: Hose
authors.affiliation: Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
authors.forename: David A
authors.initials: DA
authors.lastname: Nipperess
mesh_terms: D000818:Animals; D059001:Aquatic Organisms / Q000145:classification* / Q000235:genetics; D001315:Australia; D044822:Biodiversity; D003247:Conservation of Natural Resources / Q000379:methods*; D017753:Ecosystem; D003576:Electron Transport Complex IV / Q000235:genetics*; D060587:Groundwater; D007448:Invertebrates / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D009517:New South Wales; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D012337:RNA, Ribosomal, 18S; D003576:Electron Transport Complex IV