title: Tryptophan residue of the D-galactose/D-glucose-binding protein from E. Coli localized in its active center does not contribute to the change in intrinsic fluorescence upon glucose binding.
abstract: Changes of the characteristics of intrinsic tryptophan fluorescence of the wild type of D-galactose/D-glucose-binding protein from Escherichia coli (GGBPwt) induced by D-glucose binding were examined by the intrinsic UV-fluorescence of proteins, circular dyhroism in the near-UV region, and acrylamide-induced fluorescence quenching. The analysis of the different characteristics of GGBPwt and its mutant form GGBP-W183A together with the analysis of the microenvironment of tryptophan residues of GGBPwt revealed that Trp 183, which is directly involved in sugar binding, has the least influence on the provoked by D-glucose blue shift and increase in the intensity of protein intrinsic fluorescence in comparison with other tryptophan residues of GGBP.
authors.affiliation: Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky ave., 4, 194064, St. Petersburg, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Stepanenko
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Fonin
authors.forename: Olesya V
authors.initials: OV
authors.lastname: Stepanenko
authors.forename: Maria
authors.initials: M
authors.lastname: Staiano
authors.forename: Sabato
authors.initials: S
authors.lastname: D'Auria
authors.forename: Irina M
authors.initials: IM
authors.lastname: Kuznetsova
authors.forename: Konstantin K
authors.initials: KK
authors.lastname: Turoverov
mesh_terms: D020134:Catalytic Domain; D004926:Escherichia coli; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008958:Models, Molecular; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014364:Tryptophan
chemical_list: D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D014364:Tryptophan; D005947:Glucose; D005690:Galactose