title: Protective effects from Houttuynia cordata aqueous extract against acetaminophen-induced liver injury.
abstract: BACKGROUND
  Protective effects ofHouttuynia cordataaqueous extract (HCAE) against acetaminophen-induced hepatotoxicity in Balb/cA mice were examined.
  
  
  METHODS
  HCAE, at 1 or 2 g/L, was added into the drinking water for 4 weeks. Acute liver injury was induced by acetaminophen treatment intraperitoneally (350 mg/kg body weight).
  
  
  RESULTS
  Acetaminophen treatment significantly depleted hepatic glutathione (GSH) content, increased hepatic malonyldialdehyde (MDA), reactive oxygen species (ROS) and oxidized glutathione (GSSG) levels, and decreased hepatic activity of glutathione peroxidase (GPX), catalase and superoxide dismutase (SOD) (p<0.05). The pre-intake of HCAE alleviated acetaminophen-induced oxidative stress by retaining GSH content, decreasing MDA, ROS and GSSG production, and maintaining activity of GPX, catalase and SOD in liver (p<0.05). The pre-intake of HCAE also significantly lowered acetaminophen-induced increase in cytochrome P450 2E1 activity (p<0.05). Acetaminophen treatment increased hepatic release of interleukin (IL)-6, IL-10, tumor necrosis factor (TNF)-alpha and monocyte chemoattractant protein-1 (p<0.05). HCAE intake significantly diminished acetaminophen-induced elevation of these cytokines (p<0.05).
  
  
  CONCLUSIONS
  These results support that HCAE could provide hepato-protection.
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Rd, Taichung, Taiwan.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chen
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Rd, Taichung, Taiwan.
authors.forename: Chieh-Ling
authors.initials: CL
authors.lastname: Yang
authors.affiliation: Department of Nutrition, China Medical University, 91, Hsueh-shih Rd, Taichung, Taiwan.
authors.forename: Mei-Chin
authors.initials: MC
authors.lastname: Yin
keywords: Acetaminophen; CYP2E1; Hepatotoxicity; Houttuynia cordata; MCP-1