title: Correlation between DQB1 genetic polymorphism and genetic susceptibility in patients diagnosed with irritable bowel syndrome with diarrhea.
abstract: We examined patients of Han nationality diagnosed with irritable bowel syndrome with diarrhea (IBS-D) in Guangdong, China, to analyze the correlation between DQB1 allele polymorphisms and the genetic susceptibility to IBS-D. A total of 120 IBS-D patients of Han nationality in Guangdong, China, and 60 healthy control volunteers were included. DQB1 allele polymorphisms were investigated by polymerase chain reaction. Subjects' serum interleukin (IL)-10 level, colonic permeability, and tight junction marker zonula occludens 1 (ZO1) mRNA level were also investigated. Our data showed that the DQB1*02 allele frequency was significantly higher in IBS-D patients, while the DQB1*0603 frequency was lower than in healthy volunteers. The DQB1*03, DQB1*04, DQB1*05, DQB1*0601, DQB1*0602, and DQB1*0604 alleles did not show significant differences between IBS-D patients and healthy controls. Furthermore, patients with DQB1*03- positive and DQB1*0603-negative alleles showed more severe colonic permeability and lower serum IL-10 level and ZO1 level compared to healthy controls or even IBS-D patients with other genotypes. The present study indicated the DQB1*02 or DQB1*0603 alleles are related to IBS-D occurrence in Guangdong, China, and the mechanism of the disease may be related to reduced serum IL-10 levels.
authors.affiliation: Traditional Chinese Medicine Institute, Guangdong Medical College, GuangDong, China yujoyce022@163.com.
authors.forename: F
authors.initials: F
authors.lastname: Yu
authors.affiliation: Guangdong Provincial Hospital, University City Branch, GuangDong, China.
authors.forename: S
authors.initials: S
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Hospital, University City Branch, GuangDong, China.
authors.forename: F
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Guangdong Provincial Hospital, University City Branch, GuangDong, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Guangdong HTCM Hospital, GuangDong, China.
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.affiliation: Guangdong Provincial Hospital, University City Branch, GuangDong, China.
authors.forename: C
authors.initials: C
authors.lastname: Zheng
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003106:Colon / Q000503:physiopathology; D003967:Diarrhea / Q000150:complications* / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D020022:Genetic Predisposition to Disease; D059866:HLA-DQ beta-Chains / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood*; D043183:Irritable Bowel Syndrome / Q000235:genetics* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; C508609:IL10 protein, human; C569042:TJP1 protein, human; D062826:Zonula Occludens-1 Protein; D016753:Interleukin-10