title: Characterization of an adhesive molecule from Bacillus megaterium ADE-0-1.
abstract: An adhesive exopolysaccharide (EPS), from a biofilm forming marine strain ADE-0-1, identified as Bacillus megaterium using conventional microbiological test and 16S rDNA analysis, contained 75% carbohydrate, 17% uronic acid and 0.00125% pyruvate on dry weight basis as per colorimetric determinations and found anionic in nature by ion exchange chromatography. Paper chromatographic and HPLC analysis of EPS hydrolysate indicated presence of arabinose, glucose, mannose, galacturonic acid and glucuronic acid. Its molecular weight was 0.5×10(6) Da, by gel permeation chromatography. FT-IR spectroscopic analysis of EPS revealed presence of hydroxyl and carboxyl groups particularly. EPS exhibited an adhesive nature and could glue wood, metals and acrylic plastic. Using this EPS adhesive (10% w/v), maximum lap shear strength observed was 6.12 MPa at pH 7 and 50 °C (curing temperature) for wood to wood specimen as compared to 6.54 MPa obtained with fevicol (48 to 50% w/v).
authors.affiliation: Department of Microbiology and Biotechnology Centre, Faculty of Science, The M. S. University of Baroda, Sayajiganj, Baroda 390002, Gujarat, India. Electronic address: santoshkr.msu@gmail.com.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Microbiology and Biotechnology Centre, Faculty of Science, The M. S. University of Baroda, Sayajiganj, Baroda 390002, Gujarat, India. Electronic address: avinashkshahmsu@gmail.com.
authors.forename: Avinash K
authors.initials: AK
authors.lastname: Shah
mesh_terms: D001410:Bacillus megaterium / Q000737:chemistry* / Q000378:metabolism; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011135:Polysaccharides, Bacterial; C085953:exopolysaccharide, Bacillus
keywords: Acrylic plastic; Bacillus megaterium; Exopolysaccharide adhesive for wood; Metals