title: [Effects of astragalus and its active ingredients on ischemia reperfusion injury in isolated guinea-pig heart].
abstract: OBJECTIVE
  To explore the effects of astragalus (AST) , total flavone of astragalus (TFA), total saponins of astragalus (TSA) and astragalus polysaccharides (APS) on ischemia/reperfusion (40 min/60 min) injury in isolated guinea-pig heart.
  
  
  METHODS
  Isolated guinea-pig hearts underwent ischemia, then followed by K-H perfusion (I/R group), AST (60 mg/L),AST (60 mg/L), TFA (60 mg/L), TSA (60 mg/L) and APS (60 mg/L) perfusion (n = 6 each).Isolated hearts without ischemia serve as control group (n = 6). Activity of lactate dehydrogenas (LDH) and creatine kinase (CK) in effluent were measured.Infarct size, myocardial superoxide dismutase (SOD) activity and malondiadehyde (MDA) contents were also determined.
  
  
  RESULTS
  Compared to control hearts, heart rate, coronary flow and myocardial superoxide dismutase (SOD) activity were significantly reduced, while LDH and CK in effluent as well as myocardial MDA were significantly increased in the I/R hearts during reperfusion (all P < 0.05), these changes could be partly reversed by AST and TFA perfusion.Infarct size was also significantly reduced in AST (11.9 ± 2.03) % and TFA (13.31 ± 1.17) % treated hearts compared to that in I/R group (18.9 ± 2.27) % (all P < 0.01).
  
  
  CONCLUSIONS
  The findings indicate that AST and TFA could attenuate I/R injury in isolated guinea-pig heart possibly through enhancing the activity of SOD and reducing lipid peroxidation.
authors.affiliation: Department of Cardiology, Children's Hospital of Xuzhou, Xuzhou 221000, China.
authors.forename: Haining
authors.initials: H
authors.lastname: Zhang
authors.forename: Dongyu
authors.initials: D
authors.lastname: Min
authors.forename: Mingyu
authors.initials: M
authors.lastname: Fu
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Email: ajjsxz@163.com.
authors.forename: Xinjiang
authors.initials: X
authors.lastname: An
mesh_terms: D000818:Animals; D027883:Astragalus Plant; D006168:Guinea Pigs; D006321:Heart; D006339:Heart Rate; D017202:Myocardial Ischemia; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D009206:Myocardium; D010936:Plant Extracts / Q000494:pharmacology*; D015427:Reperfusion Injury; D013482:Superoxide Dismutase
chemical_list: D010936:Plant Extracts; D013482:Superoxide Dismutase