title: In vitro interaction of eupomatenoid-5 from Piper solmsianum C. DC. var. solmsianum and anti-tuberculosis drugs.
abstract: METHODS
  Department of Clinical Analysis and Biomedicine, State University of Maringa, Maringa, Parana, Brazil.
  
  
  OBJECTIVE
  To evaluate the in vitro interaction between eupomatenoid-5 (EUP-5), extracted from Piper solmsianum C. DC. var. solmsianum, and first-line anti-tuberculosis drugs against Mycobacterium tuberculosis H₃₇Rv and 20 clinical isolates.
  
  
  METHODS
  Resazurin drugs combination microtiter assay (REDCA) was performed to determine the interaction between EUP-5 and isoniazid, rifampicin (RMP) and ethambutol (EMB).
  
  
  RESULTS
  Synergism was observed in M. tuberculosis H₃₇Rv and eight clinical isolates with EUP-5+RMP, and in M. tuberculosis H₃₇Rv and 17 clinical isolates with EUP-5+EMB combinations.
  
  
  CONCLUSIONS
  EUP-5 is a promising compound for further studies on the development of anti-tuberculosis drugs.
authors.affiliation: Postgraduate Programme in Biosciences Applied to Pharmacy, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Lopes
authors.affiliation: Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: K R C
authors.initials: KR
authors.lastname: Ferracioli
authors.affiliation: Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: V L D
authors.initials: VL
authors.lastname: Siqueira
authors.affiliation: Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: R B
authors.initials: RB
authors.lastname: de Lima Scodro
authors.affiliation: Laboratory of Pharmacognosy, Department of Pharmacy, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: D A G
authors.initials: DA
authors.lastname: Cortez
authors.affiliation: Department of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa, Paraná, Brazil.
authors.forename: R Z
authors.initials: RZ
authors.lastname: da Silva
authors.affiliation: Postgraduate Programme in Biosciences Applied to Pharmacy, State University of Maringá, Maringá, Paraná, Brazil.
authors.forename: R F
authors.initials: RF
authors.lastname: Cardoso
mesh_terms: D000995:Antitubercular Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001572:Benzofurans / Q000302:isolation & purification / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial; D004357:Drug Synergism; D004977:Ethambutol / Q000494:pharmacology; D005838:Genotype; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D031701:Piper / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D012293:Rifampin / Q000494:pharmacology
chemical_list: D000995:Antitubercular Agents; D001572:Benzofurans; D010636:Phenols; D010936:Plant Extracts; C452811:eupomatenoid 5; D004977:Ethambutol; D007538:Isoniazid; D012293:Rifampin