title: Fabrication of nano-structured calcium silicate coatings with enhanced stability, bioactivity and osteogenic and angiogenic activity.
abstract: The bioactivity and stability of coatings on alloy implants play critical roles in the fast osseointegration and maintenance of a long-term life span of the implants, respectively. Herein, nano-sheet surface on bioactive calcium silicate (CaSiO3, CS) coatings on metal substrates was fabricated by combining atmosphere plasma spraying (APS) and hydrothermal technology (HT). The glassy phase in CS coatings generated by APS was converted into crystalline sheet-like nano-structures after HT treatment. Compared with the original CS coating samples, HT treatment decreased the degradation rate of the CS coatings. Moreover, the fabricated nano-structured topography of CS coatings increased the apatite mineralization ability and significantly enhanced the cell attachment, proliferation, differentiation, alkaline phosphatase (ALP) activity and expression of osteogenic genes and angiogenic factors of rat bone marrow stromal cells (bMSCs). Our results suggest that the nano-structured CS coatings have immense potential in improving the clinical performance of medical implants.
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Xiuhui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Stomatology, 9(th) People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Stomatology, 9(th) People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China.
authors.forename: Lunguo
authors.initials: L
authors.lastname: Xia
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Cancan
authors.initials: C
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Yi
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Chang
authors.affiliation: Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Huang
authors.affiliation: Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: xieyoutao@mail.sic.ac.cn.
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Stomatology, 9(th) People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China. Electronic address: xuyuanjin@hotmail.com.
authors.forename: Yuanjin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China. Electronic address: lklsic@mail.sic.ac.cn.
authors.forename: Kaili
authors.initials: K
authors.lastname: Lin
mesh_terms: D043925:Angiogenesis Inducing Agents / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000737:chemistry* / Q000494:pharmacology*; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017640:Silicates / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties; D013696:Temperature
chemical_list: D043925:Angiogenesis Inducing Agents; D017610:Calcium Compounds; D017640:Silicates; C031293:calcium silicate
keywords: Angiogenic activity; Calcium silicate coating; Nano-structured surface; Osteogenic activity; Stability