title: Overexpression of SCLIP promotes growth and motility in glioblastoma cells.
abstract: SCLIP, a microtubule-destabilizing phosphoprotein, is known to be involved in the development of the central nervous system (CNS). It has been well established that there are notable parallels between normal development and tumorigenesis, especially in glioma. However, no studies have examined the significance of SCLIP in gliomagenesis. To address this, we investigated the expression of SCLIP and its roles in the development of gliomas. Notably, we found that SCLIP was highly expressed in various grades of glioma samples, as compared with normal brain tissues. Overexpression of SCLIP dramatically stimulated tumor cell migration and invasion as well as proliferation and downregulation of SCLIP showed opposite effects, establishing an important oncogenic role for this gene. Furthermore, we revealed that STAT3 was required to maintain SCLIP stability, suggesting that overexpression of STAT3 may be a critical step to facilitate microtubule dynamics and subsequently promotes migration and invasion of glioma cells. Taken together, our findings demonstrate that SCLIP plays an important role in glioma pathology, and may represent a novel therapeutic strategy against human glioma.
authors.affiliation: a Key Laboratory of the Ministry of Education for Experimental Teratology; Department of Histology and Embryology ; Shandong University School of Medicine ; Jinan , China.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Shilei
authors.initials: S
authors.lastname: Ni
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.forename: Liyan
authors.initials: L
authors.lastname: Wang
authors.forename: Xianghong
authors.initials: X
authors.lastname: Zhang
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.forename: Shuai
authors.initials: S
authors.lastname: Liu
authors.forename: Aijun
authors.initials: A
authors.lastname: Hao
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D055550:Protein Stability; D050796:STAT3 Transcription Factor / Q000378:metabolism; D050777:Stathmin / Q000235:genetics*
chemical_list: D050796:STAT3 Transcription Factor; C112285:STMN3 protein, human; D050777:Stathmin
keywords: BrdU, Bromodeoxyuridine; CHX, cycloheximide; ELISA, enzyme-linked immunosorbent assay; GBM, glioblastoma; HRP, horseradish peroxidase; IHC, immunohistochemical; MTT, 3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyl-tetrazolium bromide; RT-PCR, reversed transcription polymerase chain reaction; SCLIP; SCLIP, SCG10 (superior cervical ganglia protein 10)-like protein Op18, Oncoprotein 18; STAT3; WHO, World Health Organization; glioma; growth; motility; progression; tumorigenesis