title: Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis.
abstract: OBJECTIVE
  Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.
  
  
  METHODS
  Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.
  
  
  RESULTS
  Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.
  
  
  CONCLUSIONS
  Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: William
authors.initials: W
authors.lastname: Tucker
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Susan
authors.initials: S
authors.lastname: Hannes
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Baoying
authors.initials: B
authors.lastname: Liu
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Han
authors.initials: H
authors.lastname: Si
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Ankur
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Clinical Sciences, University of Bristol, Bristol, United Kingdom.
authors.forename: Richard W J
authors.initials: RW
authors.lastname: Lee
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: H Nida
authors.initials: HN
authors.lastname: Sen
authors.affiliation: Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
authors.forename: Robert B
authors.initials: RB
authors.lastname: Nussenblatt
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000293:Adolescent; D000328:Adult; D000368:Aged; D018949:Antigens, CD1 / Q000097:blood* / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D015415:Biomarkers / Q000097:blood; D018797:Cell Cycle Proteins; D002648:Child; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000097:blood* / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D008875:Middle Aged; D022423:Myeloid Cells / Q000276:immunology*; D009687:Nuclear Proteins / Q000097:blood* / Q000276:immunology; D015534:Trans-Activators / Q000097:blood* / Q000276:immunology; D014605:Uveitis / Q000097:blood* / Q000276:immunology; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018949:Antigens, CD1; D015415:Biomarkers; C437944:CD1C protein, human; D018797:Cell Cycle Proteins; D006023:Glycoproteins; C413163:MDC1 protein, human; D009687:Nuclear Proteins; D015534:Trans-Activators
keywords: CD1c+ mDC1; CD1chi mDC1 subpopulation; autoimmune disease; noninfectious uveitis