title: Serum MicroRNAs as Diagnostic Biomarkers for Macrosomia.
abstract: BACKGROUND
  Macrosomia is defined as an infant's birth weight of more than 4000 g. Although microRNAs (miRNAs) have been implicated in the pathogenesis of various diseases, the associations between serum miRNAs and macrosomia have been rarely reported.
  
  
  METHODS
  We used the Taqman Low Density Array followed by quantitative reverse transcriptase polymerase chain reaction assays to screen for miRNAs associated with macrosomia using serum samples collected 1 week before delivery.
  
  
  RESULTS
  Profiling results showed that 1 miRNA was significantly upregulated and 10 miRNAs were significantly downregulated in serum samples of macrosomia (ΔΔCt > 3-fold). The expression levels of miR-21 were significantly decreased in macrosomia as compared to the controls in the third trimester. Receiver operating characteristic (ROC) curve analyses showed that the area under the ROC curve for miR-21 was 67.7% (sensitivity = 66.7% and specificity = 70.0%).
  
  
  CONCLUSIONS
  miR-21 in maternal serum is differentially expressed between macrosomia and controls, and miR-21 could be used as a candidate biomarker to predict macrosomia.
authors.affiliation: Perinatology Unit, Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, P.R. China jh20067034@126.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Perinatology Unit, Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, P.R. China.
authors.forename: Lingmin
authors.initials: L
authors.lastname: Hu
authors.affiliation: Perinatology Unit, Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Miao
authors.affiliation: Perinatology Unit, Changzhou Maternity and Child Health Care Hospital affiliated to Nanjing Medical University, Changzhou, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Epidemiology and Biostatistics, State Key Lab of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dong
mesh_terms: D000328:Adult; D019540:Area Under Curve; D005260:Female; D005320:Fetal Macrosomia / Q000097:blood / Q000175:diagnosis / Q000235:genetics* / Q000503:physiopathology; D020567:Fetal Weight / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005865:Gestational Age; D006801:Humans; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D012372:ROC Curve; D015203:Reproducibility of Results; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D005819:Genetic Markers; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: TLDA; biomarker; macrosomia; microRNA; serum