title: A metabolomic investigation of the effects of metal pollution in oysters Crassostrea hongkongensis.
abstract: Metal pollution has been of great concern in the estuaries in Southern China. In this study, metabolic differences between oysters Crassostrea hongkongensis from clean and metal-polluted sites were characterized using NMR-based metabolomics. We collected oyster samples from one clean (Jiuzhen) and two metal polluted sites (Baijiao and Fugong). The metal concentrations in oyster gills indicated that both the Baijiao and Fugong sites were severely polluted by several metals, including Cr, Ni, Cu, Zn, Ag, Cd and Pb. In particular, Cu and Zn were the major contaminants from the Baijiao and Fugong sites. Compared with those oysters from the clean site (JZ), metal pollution in BJ and FG induced disturbances in osmotic regulation and energy metabolism via different metabolic pathways, as indicated by different metabolic biomarkers. This study demonstrates that NMR-based metabolomics is a useful tool for characterizing metabolic responses induced by metal pollution.
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Ji
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS); Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai 264003, PR China. Electronic address: hfwu@yic.ac.cn.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of Environment and Ecology, Xiamen University, Xiamen 361102, PR China.
authors.forename: Qiaoguo
authors.initials: Q
authors.lastname: Tan
authors.affiliation: Division of Life Science, The Hong Kong University of Science and Technology (HKUST), Clearwater Bay, Kowloon, Hong Kong.
authors.forename: Wen-Xiong
authors.initials: WX
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China; D049896:Crassostrea / Q000378:metabolism*; D004784:Environmental Monitoring; D063366:Estuaries; D005880:Gills / Q000378:metabolism; D055432:Metabolomics; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D019216:Metals, Heavy; D014874:Water Pollutants, Chemical
keywords: (1)H NMR; Crassostrea hongkongensis; Gills; Metabolomics; Metal pollution