title: Antibacterial activity of Mulinum spinosum extracts against slime-producing Staphylococcus aureus and methicillin-resistant Staphylococcus aureus isolated from nasal carriers.
abstract: Nasal carriers of Staphylococcus aureus are important reservoirs with risk of developing endogenous infections or transmitting infections to susceptible individuals. Methicillin-resistant S. aureus (MRSA) are associated with higher rates of treatment failure. Some strains of S. aureus produce slime which is believed to make the microorganisms more resistant to antibiotics and host defenses. The antibacterial activity of ethyl acetate:n-hexane (EtOAc:HEX) extracts of Mulinum spinosum (5:95% EtOAc:HEX, 50:50% EtOAc:HEX, 70:30% EtOAc:HEX and mix 20:80/30:70% EtOAc:HEX, 50:50/70:30/100:0% EtOAc:HEX) were assayed against 3 slime-producing S. aureus strains and 2 MRSA strains isolated from nasal carriers. S. aureus ATCC 35556 slime-producing strain and MRSA ATCC 43300 strain were used as controls. The extracts were prepared using flash chromatography. M. spinosum 5:95% AcOEt:HEX showed antibacterial effect against all slime-producing strains (MIC: 500 µg/mL) and the highest activity against MRSA strains (MIC: 500 to 1000 µg/mL). All M. spinosum extracts assayed were active against slime-producing S. aureus and MRSA at doses between 500 and 4000 µg/mL. Both, slime-producing S. aureus and MRSA are highly contagious and hardly eradicated by antibiotic therapies. So, there is an increasing need to find new substances with the ability to inhibit these strains.
authors.affiliation: Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Echenique
authors.initials: E
authors.lastname: Daniela
authors.affiliation: Department of Chemistry, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Chiaramello
authors.initials: C
authors.lastname: Alejandra
authors.affiliation: Department of Chemistry, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Rossomando
authors.initials: R
authors.lastname: Pedro
authors.affiliation: Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Mattana
authors.initials: M
authors.lastname: Claudia
authors.affiliation: Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Alcaráz
authors.initials: A
authors.lastname: Lucía
authors.affiliation: Department of Chemistry, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Tonn
authors.initials: T
authors.lastname: Carlos
authors.affiliation: Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Laciar
authors.initials: L
authors.lastname: Analía
authors.affiliation: Department of Biochemistry and Biological Sciences, National University of San Luis, 5700 San Luis, Argentina.
authors.forename: Satorres
authors.initials: S
authors.lastname: Sara
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D019661:Apiaceae / Q000737:chemistry*; D015169:Colony Count, Microbial; D003224:Congo Red; D005798:Genes, Bacterial; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D009666:Nose / Q000382:microbiology*; D010936:Plant Extracts / Q000494:pharmacology*; D016133:Polymerase Chain Reaction
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D003224:Congo Red; D000362:Agar