title: Triggering of RNA interference with RNA-RNA, RNA-DNA, and DNA-RNA nanoparticles.
abstract: Control over cellular delivery of different functionalities and their synchronized activation is a challenging task. We report several RNA and RNA/DNA-based nanoparticles designed to conditionally activate the RNA interference in various human cells. These nanoparticles allow precise control over their formulation, stability in blood serum, and activation of multiple functionalities. Importantly, interferon and pro-inflammatory cytokine activation assays indicate the significantly lower responses for DNA nanoparticles compared to the RNA counterparts, suggesting greater potential of these molecules for therapeutic use.
authors.affiliation: Basic Research Laboratory, Center for Cancer Research, National Cancer Institute , Frederick, Maryland 21702, United States.
authors.forename: Kirill A
authors.initials: KA
authors.lastname: Afonin
authors.forename: Mathias
authors.initials: M
authors.lastname: Viard
authors.forename: Ioannis
authors.initials: I
authors.lastname: Kagiampakis
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Case
authors.forename: Marina A
authors.initials: MA
authors.lastname: Dobrovolskaia
authors.forename: Jen
authors.initials: J
authors.lastname: Hofmann
authors.forename: Ashlee
authors.initials: A
authors.lastname: Vrzak
authors.forename: Maria
authors.initials: M
authors.lastname: Kireeva
authors.forename: Wojciech K
authors.initials: WK
authors.lastname: Kasprzak
authors.forename: Vineet N
authors.initials: VN
authors.lastname: KewalRamani
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Shapiro
mesh_terms: D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D008958:Models, Molecular; D053758:Nanoparticles / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D043244:Ribonuclease III / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D034741:RNA, Small Interfering; D012313:RNA; D004247:DNA; D043244:Ribonuclease III
keywords: FRET; RNA and DNA nanoparticles; RNA and DNA nanotechnology; RNAi; RNA−DNA hybrids reassociation