title: Toscana virus genome stability: data from a meningoencephalitis case in Mantua, Italy.
abstract: In July of 2013, samples from a patient with a neurological syndrome were collected from Mantua hospital and sent to the National Reference Laboratory for Arboviruses (National Institute of Health, Rome). On the basis of the symptoms, serological and molecular assays were performed to diagnose either West Nile virus (WNV) or Toscana virus (TOSV) infection. Molecular and serological tests confirmed TOSV infection. Virus isolation was obtained from cerebrospinal fluid. A full genome sequence was determined from this TOSV strain with next-generation sequencing using Ion Torrent technology. Nucleotide and amino acidic sequences grouped phylogenetically with lineage TOSV A and showed a low genome variability.
authors.affiliation: 1 Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità , Rome, Italy .
authors.forename: Fabio
authors.initials: F
authors.lastname: Magurano
authors.forename: Melissa
authors.initials: M
authors.lastname: Baggieri
authors.forename: Gianni
authors.initials: G
authors.lastname: Gattuso
authors.forename: Claudia
authors.initials: C
authors.lastname: Fortuna
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Remoli
authors.forename: Gabriele
authors.initials: G
authors.lastname: Vaccari
authors.forename: Guendalina
authors.initials: G
authors.lastname: Zaccaria
authors.forename: Antonella
authors.initials: A
authors.lastname: Marchi
authors.forename: Paola
authors.initials: P
authors.lastname: Bucci
authors.forename: Eleonora
authors.initials: E
authors.lastname: Benedetti
authors.forename: Cristiano
authors.initials: C
authors.lastname: Fiorentini
authors.forename: Loredana
authors.initials: L
authors.lastname: Nicoletti
mesh_terms: D000328:Adult; D016679:Genome, Viral / Q000235:genetics*; D042822:Genomic Instability; D006801:Humans; D007558:Italy; D008297:Male; D008590:Meningoencephalitis / Q000175:diagnosis / Q000821:virology; D008969:Molecular Sequence Data; D010217:Phlebotomus Fever / Q000175:diagnosis / Q000821:virology; D012367:RNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics; D029301:Sandfly fever Naples virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D017423:Sequence Analysis, RNA
chemical_list: D012367:RNA, Viral
keywords: Isolation; Next-generation sequencing; Phlebovirus; Sandfly; Toscana virus