title: Post-lesion administration of 7-NI attenuated motor and non-motor deficits in 6-OHDA induced bilaterally lesioned female rat model of Parkinson's disease.
abstract: The preoperative neuroprotective effect of the 7-nitroindazole (7-NI) in 6-hydroxydopamine (6-OHDA) induced unilateral male animal models of Parkinson's disease (PD) has been widely reported. However, the therapeutic approach to PD pathology would be closely associated with the post-lesion treatment by 7-NI in 6-OHDA-induced bilateral model. Also, there is a scarcity of data on neuroprotective effect of 7-NI in PD in females. We have studied the neuroprotective effects of 7-NI in 6-OHDA-induced bilaterally lesioned female rats after short-term post-lesion treatment. Sprague-Dawley female rats with bilateral intraventricular injection of either 6-OHDA (10.5μg) (n=8-11/group) or saline (sham; n=8/group) at substantia nigra (SN) were provided with 7-NI (30mg/kg/day) intraperitoneal, once a day during the 3 consecutive days of short term treatment. 6-OHDA lesioned animals developed the motor and non-motor deficits, which were evaluated by behavioral and neuro-biochemical tests from the substantia nigra. Post-lesion administration of 7-NI reduced the motor deficits induced by 6-OHDA in the behavioral tasks such as Rota rod, open field test and forced swim test. Simultaneously, the dopamine levels were restored by 7-NI in post lesion animals up to 76% in comparison to 6-OHDA lesioned animals (23%). Furthermore, antioxidant-like effect of 7-NI was observed in lipid peroxidation, catalase, superoxide dismutase, and reduced glutathione tests. Conclusively, the present study showed that early postoperative administration of 7-NI attenuates the motor deficits induced by 6-OHDA in bilaterally lesioned female rat model of PD.
authors.affiliation: Neuropharmaceutical Research Laboratory, Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, North Campus, Mall Road, Delhi 110007, India.
authors.forename: Rita
authors.initials: R
authors.lastname: Kumari
authors.affiliation: Neuropharmaceutical Research Laboratory, Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, North Campus, Mall Road, Delhi 110007, India.
authors.forename: J B Senthil
authors.initials: JB
authors.lastname: Kumar
authors.affiliation: Neuropharmaceutical Research Laboratory, Dr. B.R. Ambedkar Centre for Biomedical Research, University of Delhi, North Campus, Mall Road, Delhi 110007, India. Electronic address: pmlsci@yahoo.com.
authors.forename: Pratibha Mehta
authors.initials: PM
authors.lastname: Luthra
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000378:metabolism; D007191:Indazoles / Q000627:therapeutic use*; D015227:Lipid Peroxidation; D009048:Motor Skills / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D016627:Oxidopamine; D010300:Parkinson Disease / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D007191:Indazoles; D018696:Neuroprotective Agents; D016627:Oxidopamine; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C080122:7-nitroindazole; D004298:Dopamine
keywords: 6-OHDA induced bilateral lesions; 7-Nitroindazole; Forced swim test; Parkinson’s disease; Post-lesion; Rota rod test