title: A qualitative and quantitative high-throughput assay for screening of gluconate high-yield strains by Aspergillus niger.
abstract: A novel two-step high-throughput strategy was developed for screening of gluconate high-yield strains by Aspergillus niger. The first step was fast qualitative assay according to the indicator color change, the second step was quantitative assay according to the absorbance of chelate formed with Cu(2+) at 810nm. The accuracy of high-throughput assay was comparable to that of high-performance liquid chromatography (HPLC). The correlation coefficient between CuSO4 assay and HPLC assays was exceeding 0.99 by statistical analysis. As a result, 3 high-yield mutants were screened out from 1000 viable single colonies, the mutants II-2-A1, IV-7-C6, and V-11-C5 were further validated in 5L of bioreactor. The average production rates were 15.5%, 32.8%, and 12.1% higher than that of the parental strain, respectively.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China. Electronic address: juchu@ecust.edu.cn.
authors.forename: Ju
authors.initials: J
authors.lastname: Chu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237 People's Republic of China.
authors.forename: Siliang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D001234:Aspergillus niger / Q000302:isolation & purification* / Q000378:metabolism*; D003124:Colorimetry / Q000379:methods*; D005942:Gluconates / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods*
chemical_list: D005942:Gluconates; C030691:gluconic acid
keywords: Aspergillus niger; Colorimetric; Gluconate production; High-throughput screening