title: Integrated protocol for reliable and fast quantification and documentation of electrophoresis gels.
abstract: Quantitative analysis of electrophoresis gels is an important part in molecular cloning, as well as in protein expression and purification. Parallel quantifications in yield and purity can be most conveniently obtained from densitometric analysis. This communication reports a comprehensive, reliable and simple protocol for gel quantification and documentation, applicable for single samples and with special features for protein expression screens. As major component of the protocol, the fully annotated code of a proprietary open source computer program for semi-automatic densitometric quantification of digitized electrophoresis gels is disclosed. The program ("GelQuant") is implemented for the C-based macro-language of the widespread integrated development environment of IGOR Pro.
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Rehbein
authors.affiliation: Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany. Electronic address: schwalbe@nmr.uni-frankfurt.de.
authors.forename: Harald
authors.initials: H
authors.lastname: Schwalbe
mesh_terms: D000818:Animals; D002138:Calibration; D003001:Cloning, Molecular; D003720:Densitometry / Q000295:instrumentation; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012015:Reference Standards; D012984:Software; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D011328:Prions; D011994:Recombinant Proteins; C100569:prion protein (121-231)
keywords: Densitometry; Expression; Purification; Quantification; SDS–PAGE; Screening