title: Fast native-SAD phasing for routine macromolecular structure determination.
abstract: We describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines.
authors.affiliation: Swiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Tobias
authors.initials: T
authors.lastname: Weinert
authors.affiliation: Swiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Vincent
authors.initials: V
authors.lastname: Olieric
authors.affiliation: Swiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Sandro
authors.initials: S
authors.lastname: Waltersperger
authors.affiliation: Swiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Ezequiel
authors.initials: E
authors.lastname: Panepucci
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Lirong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: John
authors.initials: J
authors.lastname: Rose
authors.affiliation: Laboratory of Biological Chemistry, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ebihara
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Japan.
authors.forename: Seiki
authors.initials: S
authors.lastname: Kuramitsu
authors.affiliation: Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Dianfan
authors.initials: D
authors.lastname: Li
authors.affiliation: Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Nicole
authors.initials: N
authors.lastname: Howe
authors.affiliation: Boehringer Ingelheim Pharma GmbH and Co. KG, Biberach an der Riss, Germany.
authors.forename: Gisela
authors.initials: G
authors.lastname: Schnapp
authors.affiliation: Boehringer Ingelheim Pharma GmbH and Co. KG, Biberach an der Riss, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pautsch
authors.affiliation: Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Katja
authors.initials: K
authors.lastname: Bargsten
authors.affiliation: Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Andrea E
authors.initials: AE
authors.lastname: Prota
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Parag
authors.initials: P
authors.lastname: Surana
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Jithesh
authors.initials: J
authors.lastname: Kottur
authors.affiliation: National Centre for Biological Sciences, Bangalore, India.
authors.forename: Deepak T
authors.initials: DT
authors.lastname: Nair
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
authors.forename: Federica
authors.identifier: 0000000270026316
authors.initials: F
authors.lastname: Basilico
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Cecatiello
authors.affiliation: Department of Experimental Oncology, European Institute of Oncology, Milan, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Pasqualato
authors.affiliation: Department of Biochemistry, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Boland
authors.affiliation: Department of Biochemistry, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Weichenrieder
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia, USA.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Wang
authors.affiliation: Laboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Michel O
authors.initials: MO
authors.lastname: Steinmetz
authors.affiliation: Membrane Structural and Functional Biology Group, School of Medicine and School of Biochemistry and Immunology, Trinity College Dublin, Dublin, Ireland.
authors.forename: Martin
authors.initials: M
authors.lastname: Caffrey
authors.affiliation: Swiss Light Source at Paul Scherrer Institut, Villigen, Switzerland.
authors.forename: Meitian
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D046912:Multiprotein Complexes / Q000737:chemistry*; D011487:Protein Conformation; D012984:Software; D017356:Synchrotrons; D014961:X-Ray Diffraction / Q000379:methods*
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D046912:Multiprotein Complexes