title: Detection of oligomers and fibrils of α-synuclein by AIEgen with strong fluorescence.
abstract: We report a fluorophore, TPE-TPP, with AIE characteristics which is utilized as a fluorescence probe to monitor the α-synuclein (α-Syn) fibrillation process. Compared with ThT, TPE-TPP shows a higher sensitivity in the detection of α-Syn oligomers as well as fibrils with a stronger fluorescence. The performance of TPE-TPP was evaluated using fluorescence, AFM, dot blot, and SEC.
authors.affiliation: HKUST Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park Nanshan, Shenzhen 518057, China. tangbenz@ust.hk.
authors.forename: Chris Wai Tung
authors.initials: CW
authors.lastname: Leung
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Yuning
authors.initials: Y
authors.lastname: Hong
authors.forename: Engui
authors.initials: E
authors.lastname: Zhao
authors.forename: Ryan Tsz Kin
authors.initials: RT
authors.lastname: Kwok
authors.forename: Nelson Lik Ching
authors.initials: NL
authors.lastname: Leung
authors.forename: Sijie
authors.initials: S
authors.lastname: Chen
authors.forename: Nishant N
authors.initials: NN
authors.lastname: Vaikath
authors.forename: Omar Mukhtar
authors.initials: OM
authors.lastname: El-Agnaf
authors.forename: Youhong
authors.initials: Y
authors.lastname: Tang
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Gai
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D020816:Amino Acid Motifs; D005453:Fluorescence; D005456:Fluorescent Dyes; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D013267:Stilbenes / Q000138:chemical synthesis* / Q000737:chemistry; D051844:alpha-Synuclein / Q000032:analysis* / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D013267:Stilbenes; D051844:alpha-Synuclein