title: Long non-coding RNA LSINCT5 predicts negative prognosis and exhibits oncogenic activity in gastric cancer.
abstract: Long non-coding RNAs (lncRNAs) are recently discovered RNA transcripts that are aberrantly expressed in many tumor types. Numerous studies have suggested that lncRNAs can be utilized for cancer diagnosis and prognosis. LSINCT5 (long stress-induced non-coding transcript 5) is dramatically upregulated in breast and ovarian cancer and affects cellular proliferation. However, the expression pattern of LSINCT5 in gastrointestinal cancer and the association between aberrant expression of LSINCT5 in gastrointestinal cancer and malignancy, metastasis, or prognosis remain unknown. LSINCT5 expression was detected in gastrointestinal cancer and paired adjacent normal tissue samples or cell lines using reverse transcription quantitative PCR (RT-qPCR). We also investigated the potential relationship between tumor LSINCT5 levels and clinicopathological features of gastrointestinal cancer. Finally, we assessed whether LSINCT5 influences in vitro cell proliferation. The expression of LSINCT5 is significantly upregulated in gastrointestinal cancer tissues and cell lines relative to their normal counterparts. In addition, increased LSINCT5 expression was correlated with a larger tumor size, deeper tumor depth, and advanced clinical stage. Kaplan-Meier analysis indicated that gastric cancer (GC) and colorectal cancer (CRC) patients with higher LSINCT5 expression levels have worse disease-free survival (DFS) and disease-specific survival (DSS) rates. Moreover, multivariate analysis revealed that increased expression of LSINCT5 is an independent predictor of DFS and DSS rates in GC patients. The ectopic expression of LSINCT5 in gastrointestinal cancer cell lines resulted in an increase in cellular proliferation; conversely, knock down of LSINCT5 significantly inhibited proliferation. These results suggest that LSINCT5 may represent a novel prognostic indicator and a target for gene therapy in gastrointestinal cancer.
authors.affiliation: From the Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, China (MDX, PQ, WWW, XHS, SJN, LD, DH, CT, WQS, XYZ, XD); Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China (MDX, PQ, WWW, XHS, SJN, LD, DH, CT, WQS, XYZ, XD); Institute of Pathology, Fudan University, Shanghai 200032, China (MDX, PQ, WWW, XHS, SJN, LD, DH, CT, WQS, XYZ, XD); and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China (XD).
authors.forename: Mi-Die
authors.initials: MD
authors.lastname: Xu
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Weng
authors.forename: Xiao-Han
authors.initials: XH
authors.lastname: Shen
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Ni
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.forename: Cong
authors.initials: C
authors.lastname: Tan
authors.forename: Wei-Qi
authors.initials: WQ
authors.lastname: Sheng
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm