title: Numerical and structural chromosomal anomalies in undifferentiated pleomorphic sarcoma.
abstract: BACKGROUND
  Malignant fibrous histiocytoma (MFH) or undifferentiated pleomorphic sarcoma (UPS) is the most common soft-tissue sarcoma of late adult life. Further advances in genetic characterization are warranted. The aim of this study was to search for numerical and structural chromosomal anomalies in UPS.
  
  
  METHODS
  We investigated five sarcoma-specific chromosomal translocations, five oncogene amplifications as well as the numerical karyotype of 19 UPS samples and one UPS/MFH cell line (U2197) using FISH probes on interphase nuclei.
  
  
  RESULTS
  Our results demonstrate that chromosomal translocations involving CHOP, SYT, EWS, FUS and FKHR genes are absent. Furthermore, amplification of ERBB2 (10.5%) and MDM2 (10.5%) was observed whereas the EGFR, C-MYC and N-MYC genes were not amplified. Interestingly, predominant aneuploidies were found in eight chromosomes.
  
  
  CONCLUSIONS
  The data demonstrate rarity of sarcoma-specific chromosomal breaks and oncogene amplifications in UPS, yet polysomic chromosomes appear more characteristically in this condition.
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany mustafa.becerikli@rub.de.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Becerikli
authors.affiliation: Department of Human Genetics, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Wieczorek
authors.affiliation: Institute of Pathology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Stricker
authors.affiliation: Institute of Pathology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Nambiar
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Rittig
authors.affiliation: Department of Human Genetics, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Joerg Thomas
authors.initials: JT
authors.lastname: Epplen
authors.affiliation: Institute of Pathology, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tannapfel
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Lehnhardt
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Lars
authors.initials: L
authors.lastname: Steinstraesser
authors.affiliation: Department of Plastic Surgery, BG University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Jacobsen
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D045744:Cell Line, Tumor; D019457:Chromosome Breakage; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics; D005784:Gene Amplification / Q000235:genetics*; D051677:Histiocytoma, Malignant Fibrous / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics; D051736:Proto-Oncogene Proteins c-mdm2 / Q000096:biosynthesis / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D034802:RNA-Binding Protein EWS / Q000235:genetics; D034702:RNA-Binding Protein FUS / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D051742:Transcription Factor CHOP / Q000235:genetics; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D066246:ErbB Receptors; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D011518:Proto-Oncogene Proteins; D051736:Proto-Oncogene Proteins c-mdm2; D016271:Proto-Oncogene Proteins c-myc; D034802:RNA-Binding Protein EWS; D034702:RNA-Binding Protein FUS; D018719:Erb-b2 Receptor Tyrosine Kinases; D012097:Repressor Proteins; D051742:Transcription Factor CHOP; C497249:DDIT3 protein, human; C085126:FOXO1 protein, human; C489427:MYC protein, human; C093961:SS18 protein, human; C497206:MDM2 protein, human; C512478:EGFR protein, human; C508053:ERBB2 protein, human
keywords: Chromosome aberrations; aneuploidy; fluorescent in situ hybridization; gene amplification; sarcoma