title: Screening of lactic acid bacteria that can form mixed-species biofilm with Saccharomyces cerevisiae.
abstract: The abilities of lactic acid bacteria (LAB) to form mixed-species biofilm with Saccharomyces cerevisiae in a static co-culture were investigated out of 168 LAB stock cultures, and two Lactobacillus plantarum strains (D71 and E31) and one Leuconostoc mesenteroides strain K01 were found to form mixed-species biofilm with S. cerevisiae BY4741. SEM observation showed that there was no significant difference in morphological properties among these three mixed-species biofilms and they resembled that formed by S. cerevisiae with L. plantarum ML11-11 previously isolated from a brewing sample of Fukuyama pot vinegar. The co-aggregation assays showed that L. plantarum D71 and L. plantarum E31 could co-aggregate with S. cerevisiae similarly to L. plantarum ML11-11, while L. mesenteroides K01 had no ability to co-aggregate with yeast. The above results indicate that aggregation followed by direct cell-to-cell contact is required for mixed-species biofilm formation between these L. plantarum strains and S. cerevisiae, though some different mechanism may be involved in biofilm formation between L. mesenteroides strain and S. cerevisiae.
authors.affiliation: a Department of Food Bioscience and Biotechnology, College of Bioresource Sciences , Nihon University , Fujisawa , Japan.
authors.forename: Soichi
authors.initials: S
authors.lastname: Furukawa
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Isomae
authors.forename: Noriko
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Satoru
authors.initials: S
authors.lastname: Hirayama
authors.forename: Asuka
authors.initials: A
authors.lastname: Yamagishi
authors.forename: Miho
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Chise
authors.initials: C
authors.lastname: Suzuki
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ogihara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morinaga
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D018920:Coculture Techniques; D005285:Fermentation; D048191:Lactiplantibacillus plantarum / Q000502:physiology* / Q000648:ultrastructure; D007934:Leuconostoc / Q000502:physiology* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D012441:Saccharomyces cerevisiae / Q000502:physiology* / Q000648:ultrastructure
keywords: Lactobacillus plantarum; Leuconostoc mesenteroides; Saccharomyces cerevisiae; mixed-species biofilm