title: Polyethyleneimine anchored copper(II) complexes: synthesis, characterization, in vitro DNA binding studies and cytotoxicity studies.
abstract: The water soluble polyethyleneimine-copper(II) complexes, [Cu(phen)(L-tyr)BPEI]ClO4 (where phen=1,10-phenanthroline, L-tyr=L-tyrosine and BPEI=branched polyethyleneimine) with various degree of copper(II) complex units in the polymer chain were synthesized and characterized by elemental analysis and electronic, FT-IR, EPR spectroscopic techniques. The binding of these complexes with CT-DNA was studied using UV-visible absorption titration, thermal denaturation, emission, circular dichroism spectroscopy and cyclic voltammetric methods. The changes observed in the physicochemcial properties indicated that the binding between the polymer-copper complexes and DNA was mostly through electrostatic mode of binding. Among these complexes, the polymer-copper(II) complex with the highest degrees of copper(II) complex units (higher degrees of coordination) showed higher binding constant than those with lower copper(II) complex units (lower degrees of coordination) complexes. The complex with the highest number of metal centre bound strongly due to the cooperative binding effect. Therefore, anticancer study was carried out using this complex. The cytotoxic activity for this complex on MCF-7 breast cancer cell line was determined adopting MTT assay, acridine orange/ethidium bromide (AO/EB) staining and comet assay techniques, which revealed that the cells were committed to specific mode of cell death either apoptosis or necrosis.
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Jagadeesan
authors.initials: J
authors.lastname: Lakshmipraba
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: arunasurf@yahoo.com.
authors.forename: Sankaralingam
authors.initials: S
authors.lastname: Arunachalam
authors.affiliation: Mahatma Gandi-Doerenkamp Centre, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Anvarbatcha
authors.initials: A
authors.lastname: Riyasdeen
authors.affiliation: Department of Biomedical Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Rajakumar
authors.initials: R
authors.lastname: Dhivya
authors.affiliation: Mahatma Gandi-Doerenkamp Centre, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Mohammad Abdulkader
authors.initials: MA
authors.lastname: Akbarsha
mesh_terms: D000327:Adsorption; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D002942:Circular Dichroism; D020552:Comet Assay; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000633:toxicity; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D061986:MCF-7 Cells; D009994:Osmolar Concentration; D011094:Polyethyleneimine / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D014443:Tyrosine; D003300:Copper; D011094:Polyethyleneimine; D004247:DNA; C427203:calf thymus DNA