title: Differential expression and localization of glycosidic residues in in vitro- and in vivo-matured cumulus-oocyte complexes in equine and porcine species.
abstract: Glycoprotein oligosaccharides play major roles during reproduction, yet their function in gamete interactions is not fully elucidated. Identification and comparison of the glycan pattern in cumulus-oocyte complexes (COCs) from species with different efficiencies of in vitro spermatozoa penetration through the zona pellucida (ZP) could help clarify how oligosaccharides affect gamete interactions. We compared the expression and localization of 12 glycosidic residues in equine and porcine in vitro-matured (IVM) and preovulatory COCs by means of lectin histochemistry. The COCs glycan pattern differed between animals and COC source (IVM versus preovulatory). Among the 12 carbohydrate residues investigated, the IVM COCs from these two species shared: (a) sialo- and βN-acetylgalactosamine (GalNAc)-terminating glycans in the ZP; (b) sialylated and fucosylated glycans in cumulus cells; and (c) GalNAc and N-acetylglucosamine (GlcNAc) glycans in the ooplasm. Differences in the preovulatory COCs of the two species included: (a) sialoglycans and GlcNAc terminating glycans in the equine ZP versus terminal GalNAc and internal GlcNAc in the porcine ZP; (b) terminal galactosides in equine cumulus cells versus terminal GlcNAc and fucose in porcine cohorts; and (c) fucose in the mare ooplasm versus lactosamine and internal GlcNAc in porcine oocyte cytoplasm. Furthermore, equine and porcine cumulus cells and oocytes contributed differently to the synthesis of ZP glycoproteins. These results could be attributed to the different in vitro fertilization efficiencies between these two divergent, large-animal models.
authors.affiliation: Section of Veterinary Clinics and Animal Productions, Department Emergency and Organ Transplantation (DETO), University of Bari Aldo Moro, Bari, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Accogli
authors.forename: Cécile
authors.initials: C
authors.lastname: Douet
authors.forename: Barbara
authors.initials: B
authors.lastname: Ambruosi
authors.forename: Nicola Antonio
authors.initials: NA
authors.lastname: Martino
authors.forename: Manuel Filioli
authors.initials: MF
authors.lastname: Uranio
authors.forename: Stefan
authors.initials: S
authors.lastname: Deleuze
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Dell'Aquila
authors.forename: Salvatore
authors.initials: S
authors.lastname: Desantis
authors.forename: Ghylène
authors.initials: G
authors.lastname: Goudet
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry; D006736:Horses / Q000378:metabolism*; D066298:In Vitro Techniques; D037102:Lectins; D009844:Oligosaccharides / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D013045:Species Specificity; D018709:Statistics, Nonparametric; D013552:Swine / Q000378:metabolism*; D015044:Zona Pellucida / Q000378:metabolism*
chemical_list: D037102:Lectins; D009844:Oligosaccharides