title: Synergistic radiation protective effect of purified Auricularia auricular-judae polysaccharide (AAP IV) with grape seed procyanidins.
abstract: The aim of this study was to investigate the synergistic antioxidant potential and protective effect of grape seed procyanidins (GSP) in combination with Auricularia auricular-judae polysaccharides (AAP IV) on radiation injury in splenocytes. Rat splenocyte irradiation resulted in significantly higher apoptosis rate, malondialdehyde (MDA) (p < 0.005), reactive oxygen species (ROS) (p < 0.01); cell viability, total superoxide dismutase (T-SOD) (p < 0.01), catalase (CAT) (p < 0.01), glutathione peroxidase (GSH-PX) (p < 0.05), activity and glutathione (GSH) (p < 0.01) levels were significantly reduced, compared with the control group. "GSP + AAP IV" treatment of rat splenocytes at doses of "GSP (0.3 μg/mL) + AAP IV (50 μg/mL)" displayed higher radioprotective and antioxidative effects than the administration of either GSP or AAP IV, as evident by lower levels of MDA (p < 0.001) concentration, as well as higher cell viability and T-SOD (p < 0.05), CAT (p < 0.005), GSH-PX (p < 0.01) and GSH content compared to the radiation group. In addition, in vivo studies have shown that "GSP + AAP IV" significantly ameliorated the decrease of spleen index (p < 0.005) and spleen GSH (p < 0.005) levels and significantly inhibited the increase of MDA (p < 0.005) levels of spleen with radiation-induced damage, compared with the non-treated group. The in vivo and in vitro results suggested that GSP and AAP IV have a synergistic protective effect against radiation-induced injury by improving the antioxidant and immunomodulation activities.
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. baihaina@163.com.
authors.forename: Haina
authors.initials: H
authors.lastname: Bai
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. wzy219001@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. cuijie2006@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Cui
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. sbleli@163.com.
authors.forename: Keli
authors.initials: K
authors.lastname: Yun
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. zhhua@hit.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY 14853, USA. RL23@cornell.edu.
authors.forename: Rui Hai
authors.initials: RH
authors.lastname: Liu
authors.affiliation: School of Forestry, Northeast Forestry University, 26 HeXing Road, DongLi District, Harbin 150040, China. fzl_1122@163.com.
authors.forename: Ziluan
authors.initials: Z
authors.lastname: Fan
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, 73 HuangHe Road, NanGang District, Harbin 150090, China. ccuilin@hit.edu.cn.
authors.forename: Cuilin
authors.initials: C
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004357:Drug Synergism; D062610:Fungal Polysaccharides / Q000494:pharmacology*; D056604:Grape Seed Extract / Q000494:pharmacology; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D044945:Proanthocyanidins / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D056604:Grape Seed Extract; D044945:Proanthocyanidins; D011837:Radiation-Protective Agents; D008315:Malondialdehyde