title: In vitro function of platelets treated with ultraviolet C light for pathogen inactivation: a comparative study with nonirradiated and gamma-irradiated platelets.
abstract: BACKGROUND
  During storage of platelet concentrates (PCs) replication of contaminating pathogens might occur, which can be prevented by various pathogen inactivation (PI) methods using photoactive substances in combination with ultraviolet (UV) light. A new method uses only UVC light for PI without photoactive substances. This study evaluates the in vitro function, including hemostatic properties (clot formation and elasticity), of platelets (PLTs) treated with UVC light.
  
  
  METHODS
  A PC with 35% plasma and 65% PLT additive solution (SSP+) was prepared from five buffy coats. Three PCs were pooled and divided into 3 units. One unit was used as a nonirradiated control, the second was a gamma-irradiated control, and the third unit was treated with UVC light. In vitro variables including analysis of coagulation by free oscillation rheometry were analyzed on Days 1, 5, and 7 of storage. Ten units in each group were investigated.
  
  
  RESULTS
  Swirling was well preserved, and the pH level was higher than the reference limit (6.4) during storage of PLTs in all groups. Glycolysis and PLT activation were higher for UVC-treated PLTs but the clot-forming capacity was unaffected. However, immediately after UVC treatment, the clot elastic properties were slightly affected. Hypotonic shock response decreased immediately after UVC treatment but recovered partly during the storage period.
  
  
  CONCLUSIONS
  UVC treatment affected the in vitro properties, but PLT quality and storage stability were well preserved for up to 7 days, and the in vitro hemostatic capacity of UVC-treated PLTs was only minimally altered. The clinical relevance of these changes needs to be evaluated in controlled trials.
authors.affiliation: Department of Clinical Immunology and Transfusion Medicine, Linköping University, Linköping, Sweden.|Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.|Department of Clinical Chemistry, Linköping University, Linköping, Sweden.
authors.forename: Nahreen
authors.initials: N
authors.lastname: Tynngård
authors.affiliation: Department of Clinical Immunology and Transfusion Medicine, Linköping University, Linköping, Sweden.|Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Marie
authors.initials: M
authors.lastname: Trinks
authors.affiliation: Department of Clinical Immunology and Transfusion Medicine, Linköping University, Linköping, Sweden.|Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
authors.forename: Gösta
authors.initials: G
authors.lastname: Berlin
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000528:radiation effects; D001780:Blood Coagulation Tests; D001786:Blood Glucose / Q000032:analysis; D001792:Blood Platelets / Q000502:physiology / Q000528:radiation effects*; D001793:Blood Preservation; D017848:Blood-Borne Pathogens / Q000528:radiation effects; D002245:Carbon Dioxide / Q000097:blood; D004548:Elasticity; D005720:Gamma Rays / Q000009:adverse effects; D006019:Glycolysis / Q000528:radiation effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D063847:Mean Platelet Volume; D009997:Osmotic Pressure; D010100:Oxygen / Q000097:blood; D019007:P-Selectin / Q000097:blood; D010718:Phosphatidylserines / Q000097:blood; D015539:Platelet Activation; D010979:Platelet Function Tests; D014466:Ultraviolet Rays / Q000009:adverse effects; D038301:Virus Inactivation
chemical_list: D001786:Blood Glucose; D019007:P-Selectin; D010718:Phosphatidylserines; D002245:Carbon Dioxide; D010100:Oxygen