title: Antigenic and genetic comparison of foot-and-mouth disease virus serotype O Indian vaccine strain, O/IND/R2/75 against currently circulating viruses.
abstract: Foot-and-mouth disease (FMD) virus serotype O is the most common cause of FMD outbreaks in India and three of the six lineages that have been described are most frequently detected, namely Ind2001, PanAsia and PanAsia 2. We report the full capsid sequence of 21 serotype O viruses isolated from India between 2002 and 2012. All these viruses belong to the Middle East-South Asia (ME-SA) topotype. The serological cross-reactivity of a bovine post-vaccination serum pool raised against the current Indian vaccine strain, O/IND/R2/75,was tested by virus neutralisation test with the 23 Indian field isolates, revealing a good match between the vaccine and the field isolates. The cross reactivity of the O/IND/R2/75 vaccine with 19 field isolates from other countries (mainly from Asia and Africa) revealed a good match to 79% of the viruses indicating that the vaccine strain is broadly cross-reactive and could be used to control FMD in other countries. Comparison of the capsid sequences of the serologically non-matching isolates with the vaccine strain sequence identified substitutions in neutralising antigenic sites 1 and 2, which could explain the observed serological differences.
authors.affiliation: The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, UK.
authors.forename: Mana
authors.initials: M
authors.lastname: Mahapatra
authors.affiliation: Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India.
authors.forename: S
authors.initials: S
authors.lastname: Yuvaraj
authors.affiliation: Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India.
authors.forename: M
authors.initials: M
authors.lastname: Madhanmohan
authors.affiliation: Project Directorate on Foot-and-Mouth Disease, IVRI Campus, Mukteswar-Kumaon, Nainital 263138, Uttarakhand, India.
authors.forename: S
authors.initials: S
authors.lastname: Subramaniam
authors.affiliation: Project Directorate on Foot-and-Mouth Disease, IVRI Campus, Mukteswar-Kumaon, Nainital 263138, Uttarakhand, India.
authors.forename: B
authors.initials: B
authors.lastname: Pattnaik
authors.affiliation: The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Paton
authors.affiliation: Foot-and-Mouth Disease Virus Laboratory, Research and Development Centre, Indian Immunologicals Limited, Gachibowli, Hyderabad 500 032, India. Electronic address: srinivasanva1948@gmail.com.
authors.forename: V A
authors.initials: VA
authors.lastname: Srinivasan
authors.affiliation: The Pirbright Institute, Ash Road, Woking GU24 0NF, Surrey, UK. Electronic address: satya.parida@bbsrc.ac.uk.
authors.forename: Satya
authors.initials: S
authors.lastname: Parida
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D057134:Antibodies, Neutralizing / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology; D002417:Cattle; D016000:Cluster Analysis; D003429:Cross Reactions; D029544:Foot-and-Mouth Disease Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D014644:Genetic Variation; D007194:India; D008958:Models, Molecular; D009500:Neutralization Tests; D011487:Protein Conformation; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D065288:Serogroup; D014765:Viral Vaccines / Q000235:genetics* / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D036022:Capsid Proteins; D014765:Viral Vaccines
keywords: Antigenic variation; Capsid sequence; Epitopes; FMD virus; Polyclonal antibodies