title: Exopolysaccharide production and ropy phenotype are determined by two gene clusters in putative probiotic strain Lactobacillus paraplantarum BGCG11.
abstract: Lactobacillus paraplantarum BGCG11, a putative probiotic strain isolated from a soft, white, artisanal cheese, produces a high molecular-weight heteropolysaccharide, exopolysaccharide (EPS)-CG11, responsible for the ropy phenotype and immunomodulatory activity of the strain. In this study, a 26.4-kb region originating from the pCG1 plasmid, previously shown to be responsible for the production of EPS-CG11 and a ropy phenotype, was cloned, sequenced, and functionally characterized. In this region 16 putative open reading frames (ORFs), encoding enzymes for the production of EPS-CG11, were organized in specific loci involved in the biosynthesis of the repeat unit, polymerization, export, regulation, and chain length determination. Interestingly, downstream of the eps gene cluster, a putative transposase gene was identified, followed by an additional rfb gene cluster containing the rfbACBD genes, the ones most probably responsible for dTDP-L-rhamnose biosynthesis. The functional analysis showed that the production of the high-molecular-weight fraction of EPS-CG11 was absent in two knockout mutants, one in the eps and the other in the rfb gene cluster, as confirmed by size exclusion chromatography analysis. Therefore, both eps and rfb genes clusters are prerequisites for the production of high-molecular-weight EPS-CG11 and for the ropy phenotype of strain L. paraplantarum BGCG11.
authors.forename: Milica
authors.initials: M
authors.lastname: Zivkovic
authors.forename: Marija
authors.initials: M
authors.lastname: Miljkovic
authors.forename: Patricia
authors.initials: P
authors.lastname: Ruas-Madiedo
authors.forename: Ivana
authors.initials: I
authors.lastname: Strahinic
authors.forename: Maja
authors.initials: M
authors.lastname: Tolinacki
authors.forename: Natasa
authors.initials: N
authors.lastname: Golic
authors.forename: Milan
authors.initials: M
authors.lastname: Kojic
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002611:Cheese / Q000382:microbiology*; D007778:Lactobacillus / Q000235:genetics / Q000378:metabolism*; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D010641:Phenotype; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D019936:Probiotics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011135:Polysaccharides, Bacterial