title: [Effect of manual acupuncture stimulation of "Baihui" (GV 20), etc. on serum IFN-gamma and IL-4 contents in rats with chronic fatigue syndrome].
abstract: OBJECTIVE
  To observe the effect of manual acupuncture stimulation of "Baihui" (GV 20), etc. on serum IFN- gamma and IL-4 contents in rats with chronic fatigue syndrome (CFS).
  
  
  METHODS
  A total of 24 male SD rats were equally randomized into control group, model group and acupuncture group. CFS model was established by bounding and forced swimming in cold water once daily for 14 days. Rats in the acupuncture group were treated by manual acupuncture stimulation of bilateral "Zusanli" (ST 36), "Baihui" (GV 20), and "Guanyuan" (CV 4), once daily for 14 days. Serum IFN-gamma and IL-4 contents were detected by ELISA.
  
  
  RESULTS
  Compared with the control group, the contention of serum IFN-gamma and ratio of IFN-gamma/IL-4 were significant decreased in the model group (P<0.01). While in comparison with the model group, the contention of IFN-gamma and ratio of IFN-gamma/IL-4 were obviously increased in the acupuncture group (P<0.05). No significant differences were found among the three groups in serum IL-4 levels (P>0.05).
  
  
  CONCLUSIONS
  Manual acupuncture can inhibit CFS induced reduction of serum IFN-gamma level and the ratio of IFN-gamma/IL-4 in CFS rats, suggesting a favorable adjustment of acupuncture intervention for CFS by balancing the ratio of IFN-gamma/IL-4.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Wen-juan
authors.initials: WJ
authors.lastname: Xie
authors.forename: Mi
authors.initials: M
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.forename: Jia-li
authors.initials: JL
authors.lastname: Zhang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Liu
authors.forename: Li-na
authors.initials: LN
authors.lastname: Guo
mesh_terms: D015669:Acupuncture Points; D015670:Acupuncture Therapy / Q000379:methods; D000818:Animals; D015673:Fatigue Syndrome, Chronic / Q000097:blood / Q000276:immunology / Q000628:therapy*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D015847:Interleukin-4 / Q000097:blood* / Q000276:immunology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D015847:Interleukin-4; D007371:Interferon-gamma