title: Monitoring clinical levels of heparin in human blood samples with an indicator-displacement assay.
abstract: We report that a "tree-like" polymer of lysine is able to form a multi-ligand complex with a fluorescently labelled peptide, leading to the almost complete extinction of the optical signal that can be restored upon the introduction of heparin. This simple system allows, for the first time, the turn-ON fluorescent sensing of the anticoagulant in human blood at clinically relevant levels.
authors.affiliation: Institut des Biomolécules Max Mousseron, UMR 5247 CNRS - Université de Montpellier, Place Eugène Bataillon, cc 1706, 34296 Montpellier cedex 5, France. laurent.vial@univ-montp2.fr.
authors.forename: Jean-Patrick
authors.initials: JP
authors.lastname: Francoia
authors.forename: Robert
authors.initials: R
authors.lastname: Pascal
authors.forename: Laurent
authors.initials: L
authors.lastname: Vial
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006493:Heparin / Q000097:blood*; D006801:Humans; D008239:Lysine / Q000737:chemistry; D008958:Models, Molecular
chemical_list: D005456:Fluorescent Dyes; D006493:Heparin; D008239:Lysine