title: The Cell Survival of F10B16 Melanoma and 4T1 Breast Adenocarcinoma Irradiated to Gamma Radiation Using the MTT Assay Based on Two Different Calculation Methods.
abstract: BACKGROUND
  various MTT assay methods are proposed to obtain the cell survival parameters.
  
  
  OBJECTIVE
  determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation.
  
  
  METHODS
  a common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast adenocarcinoma survivals after exposing them to ionizing radiation from a Co-60 machine. To obtain the survival parameters of the cells based on the common method, the cells were inoculated in 96-well plates. After irradiating the plates, the MTT assay was performed over the following days for a period of 8 days. Thereafter, the survival fraction was calculated from a simple equation for every day from which the best day was selected. To acquire the cells' survival parameters based on the novel method, extensive experiments were performed on a large number of samples. Then, the MTT assay was done in every day following various experimental treatments to acquire the exponential growth. Finally, the cells' survivals were determined by measuring the space between relevant growing curves.
  
  
  RESULTS
  at low doses (<4Gy) the two MTT assay methods indicated the same results. However, at higher doses there were significant differences among the findings.
  
  
  CONCLUSIONS
  both of the MTT methods indicated that the cells' responses are dependent on the dose levels used. Although the implementation of the common MTT assay method is simpler, the novel method seems to show more precise and reliable results at all levels of radiation doses.
authors.affiliation: Ph.D. Candidate, Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;
authors.forename: Safoora
authors.initials: S
authors.lastname: Nikzad
authors.affiliation: Associate Professor, Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;
authors.forename: Bijan
authors.initials: B
authors.lastname: Hashemi
authors.affiliation: Professor, Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran;
authors.forename: Zuhair-Mohammad
authors.initials: ZM
authors.lastname: Hassan
authors.affiliation: Professor, Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mozdarani
keywords: 4T1 breast adenocarcinoma; F10B16 melanoma; MTT assay; cell survival fraction; growing curve; ionizing radiation