title: Multiphoton microscopic imaging of rabbit dorsal skin.
abstract: Rabbits are often preferred to be experimental animals during the skin research. The visualizing and understanding the full-thickness structure of rabbit skin has significance in biology, medicine, and animal husbandry. In this study, multiphoton microscopy (MPM) was employed to examine the rabbit skin on the back, which was based on second harmonic generation and two-photon excited fluorescence. High-resolution images were achieved from the fresh, unfixed, and unstained tissues, showing detailed microstructure of the skin without the administration of exogenous contrast agents. The morphology and distribution of the main components of epidermis and dermis, such as keratin, collagen fibers, elastic fibers, and hair follicles, can be distinctly identified in MPM images. Since the changes in these components are tightly related to skin diseases and wound healing, the noninvasive nature of MPM enables it become a valuable tool in skin research for detecting and monitoring.
authors.affiliation: Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, P. R. China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zhu
authors.forename: Yahao
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Hong
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Shuangmu
authors.initials: S
authors.lastname: Zhuo
authors.forename: Jianxin
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011817:Rabbits; D012867:Skin / Q000033:anatomy & histology*
keywords: multiphoton microscopy; rabbit skin; second harmonic generation; two-photon excited fluorescence