title: Molecular cloning, expression, purification and characterization of vitellogenin in scallop Patinopecten yessoensis with special emphasis on its antibacterial activity.
abstract: Vitellogenin (Vg), the major precursor of the egg-yolk proteins, has been found to play an immune role in fish and protochordate amphioxus, however, no study on the immune function of Vg in invertebrates has ever been studied before. In this study, the complete cDNA of Vg was identified from the scallop Patinopecten yessoensis (termed PyVg). The cDNA contained an open reading frame (ORF) of 6888 bp, encoding a polypeptide of 2295 amino acid protein, which had an N-terminal signal peptide followed by the mature Vg. The mature Vg had the domains Vitellogenin_N, domain of unknown function 1943 (DUF1943) and von Willebrand factor type D domain (VWD) as well as the consensus cleavage site (R-X-R/K-R) and conserved motif (KTIGNAG). Tissue distribution assay revealed that PyVg transcripts were predominantly present in the ovary and hepatopancreas, and its expression profile in ovary well reflected the annual cycle of vitellogenesis. Interestingly, bacterial challenge caused a significant change in PyVg expression, hinting an involvement of PyVg in the acute phase response in P. yessoensis. Consistently, recombinant DUF1943 and VWD domains both could interact with LTA and LPS on bacterial wall, and purified native PyVg displayed a broad-spectrum antibacterial activity against both Gram-negative (Escherichia coli and Vibrio anguillarum) and Gram-positive bacteria (Staphylococcus aureus). Overall, these data indicate that Vg is a pattern recognition molecule with bacterial growth-inhibiting activity in the scallop.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Qingdao 266003, China; Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine Biology, Ocean University of China, Qingdao 266003, China. Electronic address: jamesdong@ouc.edu.cn.
authors.forename: Guangdong
authors.initials: G
authors.lastname: Ji
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. Electronic address: yangag@ysfri.ac.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D020869:Gene Expression Profiling; D043143:Hepatopancreas / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010053:Ovary / Q000378:metabolism; D049897:Pectinidae / Q000235:genetics / Q000276:immunology*; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000276:immunology; D013682:Teichoic Acids / Q000378:metabolism; D014733:Vibrio / Q000276:immunology; D014818:Vitellogenesis; D014819:Vitellogenins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D013682:Teichoic Acids; D014819:Vitellogenins; C009900:lipoteichoic acid
keywords: Antibacterial activity; Patinopecten yessoensis; Pattern recognition receptor; Scallop; Vitellogenin