title: Pokemon (FBI-1) interacts with Smad4 to repress TGF-β-induced transcriptional responses.
abstract: Pokemon, an important proto-oncoprotein, is a transcriptional repressor that belongs to the POK (POZ and Krüppel) family. Smad4, a key component of TGF-β pathway, plays an essential role in TGF-β-induced transcriptional responses. In this study, we show that Pokemon can interact directly with Smad4 both in vitro and in vivo. Overexpression of Pokemon decreases TGF-β-induced transcriptional activities, whereas knockdown of Pokemon increases these activities. Interestingly, Pokemon does not affect activation of Smad2/3, formation of Smads complex, or DNA binding activity of Smad4. TGF-β1 treatment increases the interaction between Pokemon and Smad4, and also enhances the recruitment of Pokemon to Smad4-DNA complex. In addition, we also find that Pokemon recruits HDAC1 to Smad4 complex but decreases the interaction between Smad4 and p300/CBP. Taken together, all these data suggest that Pokemon is a new partner of Smad4 and plays a negative role in TGF-β pathway.
authors.affiliation: Department of Neurobiology, Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, Beijing,100069, China. Electronic address: yutaoy@ccmu.edu.cn.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, 45267, USA; Institute of Disease Control and Prevention, Chinese Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Cui
authors.affiliation: Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xue
authors.affiliation: Institute of Disease Control and Prevention, Chinese Academy of Military Medical Sciences, Beijing, 100071, China.
authors.forename: Chuanfu
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Neurobiology, Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, Beijing,100069, China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, 45267, USA.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Bi
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Shan
authors.affiliation: Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, 45267, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Meredith
authors.affiliation: Department of Molecular and Biomedical Pharmacology, University of Kentucky College of Medicine, Lexington KY, 40536, USA.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Beijing Key Laboratory of Major Brain Disorders, Capital Medical University, Beijing,100069, China. Electronic address: zhiqingx@ccmu.edu.cn.
authors.forename: Zhi-Qing David
authors.initials: ZQ
authors.lastname: Xu
mesh_terms: D001665:Binding Sites; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D060066:Protein Interaction Maps / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics; D051899:Smad2 Protein / Q000235:genetics / Q000378:metabolism; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism; D051901:Smad4 Protein / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D004268:DNA-Binding Proteins; C497782:SMAD2 protein, human; C497796:SMAD4 protein, human; D051899:Smad2 Protein; D051900:Smad3 Protein; D051901:Smad4 Protein; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; C485514:ZBTB7A protein, human
keywords: Interaction; Pokemon; Smad4; TGF-β pathway