title: [Apoptin enhances the oncolytic activity of vaccinia virus].
abstract: Chicken anemia virus gene encoding apoptin, a selective killer of cancer cells was synthesized and inserted into vaccinia virus (strain L-IVP) genome. The insertion has replaced major part of the viral C11R gene encoding viral growth factor (VGF), which is important for the virulence. The recombinant virus VVdGF-ApoS24/2 was obtained through the transient dominant selection technique with the use of puromycin resistance gene as the selective marker. The expression apoptin gene from a synthetic early-late promoter of vaccinia virus effectively provides accumulation of the protein in the cells infected with the VVdGF-ApoS24/2 virus. Despite the presence of virus growth factor signal peptide at apoptin N-terminal secretion of the recombinant protein into culture medium did not occur. The recombinant virus VVdGF-ApoS24/2 was found to have a significantly greater selective lyticactivity on human cancer cell lines (A549, A431, U87MG, RD and MCF7) as compared with the parent strain L-IVP and its variant VVdGF2/6 with the deletion of the C11R gene. The results suggest that the use of apoptin represents a promising approach for improving the natural anticancer activities of vaccinia virus.
authors.forename: G V
authors.initials: GV
authors.lastname: Kochneva
authors.forename: I N
authors.initials: IN
authors.lastname: Babkina
authors.forename: T A
authors.initials: TA
authors.lastname: Lupan
authors.forename: A A
authors.initials: AA
authors.lastname: Grazhdantseva
authors.forename: P V
authors.initials: PV
authors.lastname: Iudin
authors.forename: G F
authors.initials: GF
authors.lastname: Sivolobova
authors.forename: A N
authors.initials: AN
authors.lastname: Shvalov
authors.forename: E G
authors.initials: EG
authors.lastname: Popov
authors.forename: I V
authors.initials: IV
authors.lastname: Babkin
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: P M
authors.initials: PM
authors.lastname: Chumakov
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000235:genetics*; D036022:Capsid Proteins / Q000235:genetics* / Q000627:therapeutic use; D017727:Chicken anemia virus / Q000235:genetics; D002645:Chickens / Q000235:genetics / Q000821:virology; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D061986:MCF-7 Cells; D009369:Neoplasms / Q000235:genetics* / Q000628:therapy / Q000821:virology; D050130:Oncolytic Virotherapy / Q000379:methods; D050504:Oncolytic Viruses / Q000235:genetics*; D014616:Vaccinia virus / Q000235:genetics*; D014779:Virus Replication / Q000235:genetics
chemical_list: D019496:Cancer Vaccines; D036022:Capsid Proteins; C091717:VP3 protein, Chicken anemia virus