title: Corynebacterium pseudotuberculosis cp09 mutant and cp40 recombinant protein partially protect mice against caseous lymphadenitis.
abstract: BACKGROUND
  Caseous lymphadenitis (CLA) is an infectious disease that affects small ruminants and is caused by Corynebacterium pseudotuberculosis. This disease is responsible for high economic losses due to condemnation and trim of infected carcasses, decreased leather and wool yield, loss of sales of breeding stock and deaths from internal involvement. Treatment is costly and ineffective; the most cost-effective strategy is timely immunisation. Various vaccine strategies have been tested, and recombinant vaccines are a promising alternative. Thus, in this study, different vaccine formulations using a recombinant protein (rCP40) and the CP09 live recombinant strain were evaluated. Five groups of 10 mice each were immunised with saline (G1), rCP40 (G2), CP09 (G3), a combination of CP09 and rCP40 (G4) and a heterologous prime-boost strategy (G5). Mice received two immunisations within 15 days. On day 30 after primary immunisation, all groups were challenged with a C. pseudotuberculosis virulent strain. Mice were monitored and mortality was recorded for 30 days after challenge.
  
  
  RESULTS
  The G2, G4 and G5 groups showed high levels of IgG1 and IgG2a; G2 presented significant IgG2a production after virulent challenge in the absence of IgG1 and IgG3 induction. Thirty days after challenge, the mice survival rates were 20 (G1), 90 (G2), 50 (G3), 70 (G4) and 60% (G5).
  
  
  CONCLUSIONS
  rCP40 is a promising target in the development of vaccines against caseous lymphadenitis.
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. judsonall_16@hotmail.com.
authors.forename: Judson W
authors.initials: JW
authors.lastname: Silva
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. danieladroppa@gmail.com.
authors.forename: Daniela
authors.initials: D
authors.lastname: Droppa-Almeida
authors.affiliation: Biotechnology Unit/Center for Technology Development, Federal University of Pelotas, Capão do Leão, Rio Grande do Sul, 96010-900, Brazil. sibeleborsuk@gmail.com.
authors.forename: Sibele
authors.initials: S
authors.lastname: Borsuk
authors.affiliation: Biological Sciences Institute, General Biology Department, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Minas Gerais, Belo Horizonte, Brazil. vascoariston@gmail.com.
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
authors.affiliation: Health Sciences Institute, Federal University of Bahia, Avenida Reitor Miguel Calmon s/n, Salvador, BA, 40110-100, Brazil. rwportela@gmail.com.
authors.forename: Ricardo W
authors.initials: RW
authors.lastname: Portela
authors.affiliation: Biological Sciences Institute, General Biology Department, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Minas Gerais, Belo Horizonte, Brazil. miyoshi@icb.ufmg.br.
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.affiliation: Biological Sciences Institute, General Biology Department, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Minas Gerais, Belo Horizonte, Brazil. flasouz@yahoo.com.br.
authors.forename: Flávia S
authors.initials: FS
authors.lastname: Rocha
authors.affiliation: Biological Sciences Institute, General Biology Department, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, Minas Gerais, Belo Horizonte, Brazil. fernandadorella@gmail.com.
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. lordelovivas@yahoo.com.br.
authors.forename: Wanessa L
authors.initials: WL
authors.lastname: Vivas
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. fpadilha@yahoo.com.
authors.forename: Francine F
authors.initials: FF
authors.lastname: Padilha
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. lucyherma@hotmail.com.
authors.forename: Maria L
authors.initials: ML
authors.lastname: Hernández-Macedo
authors.affiliation: Technology and Research Institute, Tiradentes University, Av. Murilo Dantas, 300, Aracaju, Sergipe, 49032-490, Brazil. isabel_limaverde@yahoo.com.br.
authors.forename: Isabel B
authors.initials: IB
authors.lastname: Lima-Verde
mesh_terms: D000818:Animals; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D003354:Corynebacterium Infections / Q000382:microbiology / Q000517:prevention & control*; D016925:Corynebacterium pseudotuberculosis / Q000235:genetics* / Q000276:immunology; D004269:DNA, Bacterial / Q000235:genetics; D008199:Lymphadenitis / Q000382:microbiology / Q000517:prevention & control*; D051379:Mice / Q000276:immunology / Q000382:microbiology; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D014614:Vaccines, Synthetic / Q000627:therapeutic use*
chemical_list: D001428:Bacterial Vaccines; D004269:DNA, Bacterial; D014614:Vaccines, Synthetic