title: Electrografted diazonium salt layers for antifouling on the surface of surface plasmon resonance biosensors.
abstract: Electrografted diazonium salt layers on the surface of surface plasmon resonance (SPR) sensors present potential for a significant improvement in antifouling coatings. A pulsed potential deposition profile was used in order to circumvent mass-transport limitations for layer deposition rate. The influence of number of pulses with respect to antifouling efficacy was evaluated by nonspecific adsorption surface coverage of crude bovine serum proteins. Instead of using empirical and rough estimated values, the penetration depth and sensitivity of the SPR instrument were experimentally determined for the calculation of nonspecific adsorption surface coverage. This provides a method to better examine antifouling surface coatings and compare crossing different coatings and experimental systems. Direct comparison of antifouling performance of different diazonium salts was facilitated by a tripad SPR sensor design. The electrografted 4-phenylalanine diazonium chloride (4-APhe) layers with zwitterionic characteristic demonstrate ultralow fouling.
authors.affiliation: Department of Chemistry and Biochemistry, University of Delaware , Newark, Delaware 19716, United States.
authors.forename: Qiongjing
authors.initials: Q
authors.lastname: Zou
authors.forename: Laurel L
authors.initials: LL
authors.lastname: Kegel
authors.forename: Karl S
authors.initials: KS
authors.lastname: Booksh
mesh_terms: D000818:Animals; D057219:Biofouling; D002417:Cattle; D003979:Diazonium Compounds / Q000737:chemistry*; D015394:Molecular Structure; D012492:Salts / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis*; D020349:Surface Plasmon Resonance; D013499:Surface Properties
chemical_list: D003979:Diazonium Compounds; D012492:Salts; D012710:Serum Albumin, Bovine