title: The open, the closed, and the empty: time-resolved fluorescence spectroscopy and computational analysis of RC-LH1 complexes from Rhodopseudomonas palustris.
abstract: We studied the time-resolved fluorescence of isolated RC-LH1 complexes from Rhodopseudomonas palustris as a function of the photon fluence and the repetition rate of the excitation laser. Both parameters were varied systematically over 3 orders of magnitude. On the basis of a microstate description we developed a quantitative model for RC-LH1 and obtained very good agreement between experiments and elaborate simulations based on a global master equation approach. The model allows us to predict the relative population of RC-LH1 complexes with the special pair in the neutral state or in the oxidized state P(+) and those complexes that lack a reaction center.
authors.affiliation: Experimental Physics IV and Bayreuther Institut für Makromolekülforschung (BIMF), University of Bayreuth , 95440 Bayreuth, Germany.
authors.forename: Sebastian R
authors.initials: SR
authors.lastname: Beyer
authors.forename: Lars
authors.initials: L
authors.lastname: Müller
authors.forename: June
authors.initials: J
authors.lastname: Southall
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Cogdell
authors.forename: G Matthias
authors.initials: GM
authors.lastname: Ullmann
authors.forename: Jürgen
authors.initials: J
authors.lastname: Köhler
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D003198:Computer Simulation; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry*; D012241:Rhodopseudomonas / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes