title: Hairpin DNA as a biobarcode modified on gold nanoparticles for electrochemical DNA detection.
abstract: Hairpin DNA (hpDNA) as a novel biobarcode was conjugated with gold nanoparticles (AuNPs) and a reporter DNA (rpDNA) to form hpDNA/AuNP/rpDNA nanoparticles for the detection of an oligonucleotide sequence associated with Helicobacter pylori as a model target. The rpDNA is complementary to about a half-portion of the target DNA sequence (tDNA). A capture DNA probe (cpDNA), complementary to the other half of the tDNA, was immobilized on the surface of a gold electrode. In the presence of tDNA, a sandwich structure of (hpDNA/AuNP/rpDNA)/tDNA/cpDNA was formed on the electrode surface. The differential pulse voltammetry (DPV) detection was based on [Ru(NH3)5(3-(2-phenanthren-9-yl-vinyl)-pyridine)](2+), an electroactive complex that binds to the sandwich structure by its intercalation with the hpDNA and the double-stranded DNA (dsDNA) of the sandwich structure. The several factors--high density of biobarcode hpDNA on the surface of AuNPs, multiple electroactive complex molecules intercalated with each hpDNA and dsDNA molecule, and the intercalation binding mode of the electroactive complex with the DNA sandwich structure--contribute to the DNA sensor with highly selective and sensitive sensing properties. The DNA sensor exhibited a detection limit of 1 × 10(-15) M (i.e., 1 fM), the DNA levels in physiological samples, with linearity down to 2 × 10(-15) M. It can differentiate even one single mismatched DNA from the complementary tDNA. This novel biobarcode-based DNA sensing approach should provide a general platform for development of direct, simple, repetitive, sensitive, and selective DNA sensors for various important applications in analytical, environmental, and clinical chemistry.
authors.affiliation: Bioengineering Department, School of Life Sciences, Zhengzhou University , 100# Science Avenue, Zhengzhou, 450001, People's Republic of China.
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Cui
authors.forename: Tai-Bin
authors.initials: TB
authors.lastname: Xu
authors.forename: Yu-Long
authors.initials: YL
authors.lastname: Sun
authors.forename: An-Wei
authors.initials: AW
authors.lastname: Zhou
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Cui
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: John H T
authors.initials: JH
authors.lastname: Luong
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry*; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D055664:Electrochemical Techniques / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry*; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D015342:DNA Probes; D004269:DNA, Bacterial; D061925:Immobilized Nucleic Acids; D006046:Gold; D012428:Ruthenium