title: TIMP-1 expression in human colorectal cancer is associated with SMAD3 gene expression levels: a pilot study.
abstract: The prognosis of colorectal cancer (CRC) varies considerably, and there is a compelling need to identify novel biomarkers with prognostic significance. The aim of the present study was to evaluate the prognostic value of a panel of six genes (CDH1, SMAD3, TGFβ1, ICAM-1, TIMP-1 and MUC12) in CRC patients. METHODS. We evaluated these genes by qRT-PCR in normal and CRC tumor tissue, and correlated the relative gene expression values with clinical, pathological aspects and other biological factors. RESULTS. RNA expression levels of CDH1, SMAD3, TGFβ1, ICAM-1, TIMP-1 and MUC12 were measured by qRT-PCR in a set of 39 tumor samples and non-cancer tissue. Statistically significant increases in expression levels were found for ICAM-1 and TIMP-1 when comparing tumor samples to the non-tumor group. CONCLUSIONS. Among the genes which displayed differential expressions between tumor tissue and adjoining normal tissue, the ones that presented statistically significant correlations were TIMP-1 and SMAD3, possibly with prognostic significance.
authors.affiliation: Department of Surgery, Iuliu Hatieganu University of Medicine and Pharmacy; Surgery Clinic 1, County Hospital, Cluj-Napoca, Romania.
authors.forename: Calin
authors.initials: C
authors.lastname: Ionescu
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania. cornelia.braicu@umfcluj.ro.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Department of Dermatology, University of Freiburg, Freiburg, Germany.
authors.forename: Roxana
authors.initials: R
authors.lastname: Chiorean
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca; Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania.
authors.forename: Roxana
authors.initials: R
authors.lastname: Cojocneanu Petric
authors.affiliation: Department of Surgery, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca, Romania.
authors.forename: Emilian
authors.initials: E
authors.lastname: Neagoe
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania.
authors.forename: Laura
authors.initials: L
authors.lastname: Pop
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania.
authors.forename: Sergiu
authors.initials: S
authors.lastname: Chira
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca; Department of Functional Genomics and Experimental Pathology, Prof. Dr. I. Chiricuta Oncology Institute, Cluj-Napoca; Department of Immunology, Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011237:Predictive Value of Tests; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction; D051900:Smad3 Protein / Q000235:genetics*; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C497790:SMAD3 protein, human; D051900:Smad3 Protein; C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1