title: Rapid determination of fenoldopam in human plasma by UPLC-MS/MS for pharmacokinetic analysis in patients.
abstract: We developed and validated a rapid, selective, and sensitive ultra-performance liquid-chromatography mass-spectrometry (UPLC-MS/MS) method for quantifying fenoldopam in human plasma for pharmacokinetic studies. Fenoldopam and the internal-standard (IS), oxazepam, were isolated from human plasma by liquid-liquid extraction using ethyl acetate after alkalization, and were separated on a 2.1×100 mm Acquity UPLC HSS T3 C18 column (inside diameter, 1.8 μm) using a mobile phase of water (0.05% formic acid) and acetonitrile gradient elution. The fenoldopam and IS were eluted at 1.07 and 2.32 min, respectively. Quantification was performed using positive-ion electrospray-ionization (ESI), and the fenoldopam and IS responses were optimized at the m/z 306.16→107.10 and m/z 287.1→241.01 transitions, respectively. The assay was validated over the linear range of 0.1-40 ng/mL fenoldopam with intra- and interassay precision <13.21%. The matrix effect of normal and hemolyzed plasma was 94.9-101.6%. Fenoldopam was stable for ≥34 days at -70 °C in normal and hemolyzed plasma containing ascorbic acid as a stabilizer. This method can be successfully applied in pharmacokinetic studies of fenoldopam in hypertensive patients.
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Xipei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: He
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Mai
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Shilong
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Qiuxiong
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Shan
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
authors.forename: Chunyu
authors.initials: C
authors.lastname: Deng
authors.affiliation: Department of Pharmacy, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. Electronic address: mnmyang@aliyun.com.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Guangdong General Hospital Medical Research Center, Guangdong Cardiovascular Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. Electronic address: yuxycn@hotmail.com.
authors.forename: Xiyong
authors.initials: X
authors.lastname: Yu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D018818:Fenoldopam / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D059625:Liquid-Liquid Extraction; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018818:Fenoldopam
keywords: Fenoldopam; Hemolyzed plasma; Human plasma; Pharmacokinetics; UPLC–MS/MS