title: Assessment of the European flounder responses to chemical stress in the English Channel, considering biomarkers and life history traits.
abstract: A multi-biomarker approach was developed to evaluate responses of European flounder (Platichthys flesus) in three contrasted estuaries over the English Channel: the Canche (pristine site), Tamar (heavy metals and PAHs contamination) and Seine (heavily pollution with a complex cocktail of contaminants). The condition factor and several biomarkers of the immune system, antioxidant enzymes, energetic metabolism and detoxification processes were investigated in young-of-the-year (0+) and one-year-old (1+) flounder. Results underlined the difference between the pristine site and the Seine estuary which showed a lower condition factor, a modulation of the immune system, a higher Cytochrome C oxidase activity, and an up-regulation of BHMT expression. The moderate biomarker responses in the Tamar fish could be linked to the specific contamination context of this estuary. Flounder life history traits were analyzed by otolith microchemistry, in order to depict how the fish use their habitat and thus respond to chemical stress in estuaries.
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France; Institut National de la Recherche Scientifique, Institut Armand-Frappier (INRS-IAF), 31 Boulevard des Prairies, Laval, Québec H7V 1B7, Canada. Electronic address: cel.e@hotmail.fr.
authors.forename: Célie
authors.initials: C
authors.lastname: Dupuy
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Galland
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France.
authors.forename: Vianney
authors.initials: V
authors.lastname: Pichereau
authors.affiliation: Institut National de l'Environnement Industriel et des Risques, Pôle "Dangers et impact sur le vivant", Unité d'écotoxicologie in vitro et in vivo, Parc Technologique ALATA, 60550 Verneuil en Halatte, France.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Sanchez
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Riso
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France; Centre IRD de Bretagne, BP 70, 29280 Plouzané, France.
authors.forename: Maylis
authors.initials: M
authors.lastname: Labonne
authors.affiliation: Université Lille Nord de France, ULCO LOG, CNRS UMR 8187, F-62930 Wimereux, France.
authors.forename: Rachid
authors.initials: R
authors.lastname: Amara
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Charrier
authors.affiliation: Institut National de la Recherche Scientifique, Institut Armand-Frappier (INRS-IAF), 31 Boulevard des Prairies, Laval, Québec H7V 1B7, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Fournier
authors.affiliation: Université Européenne de Bretagne, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Laboratoire des Sciences de l'Environnement Marin LEMAR, Institut Universitaire Européen de la Mer, 29280 Plouzané, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Laroche
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D017753:Ecosystem; D004784:Environmental Monitoring; D063366:Estuaries; D005060:Europe; D005432:Flounder / Q000378:metabolism / Q000502:physiology*; D008018:Life Cycle Stages; D019216:Metals, Heavy / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000378:metabolism / Q000633:toxicity; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical
keywords: Estuaries; Fish; Gene expression; Immunotoxicity; Life-history traits; Pollution