title: Characterization of the uncertainty of divergence time estimation under relaxed molecular clock models using multiple loci.
abstract: Genetic sequence data provide information about the distances between species or branch lengths in a phylogeny, but not about the absolute divergence times or the evolutionary rates directly. Bayesian methods for dating species divergences estimate times and rates by assigning priors on them. In particular, the prior on times (node ages on the phylogeny) incorporates information in the fossil record to calibrate the molecular tree. Because times and rates are confounded, our posterior time estimates will not approach point values even if an infinite amount of sequence data are used in the analysis. In a previous study we developed a finite-sites theory to characterize the uncertainty in Bayesian divergence time estimation in analysis of large but finite sequence data sets under a strict molecular clock. As most modern clock dating analyses use more than one locus and are conducted under relaxed clock models, here we extend the theory to the case of relaxed clock analysis of data from multiple loci (site partitions). Uncertainty in posterior time estimates is partitioned into three sources: Sampling errors in the estimates of branch lengths in the tree for each locus due to limited sequence length, variation of substitution rates among lineages and among loci, and uncertainty in fossil calibrations. Using a simple but analogous estimation problem involving the multivariate normal distribution, we predict that as the number of loci ([Formula: see text]) goes to infinity, the variance in posterior time estimates decreases and approaches the infinite-data limit at the rate of 1/[Formula: see text], and the limit is independent of the number of sites in the sequence alignment. We then confirmed the predictions by using computer simulation on phylogenies of two or three species, and by analyzing a real genomic data set for six primate species. Our results suggest that with the fossil calibrations fixed, analyzing multiple loci or site partitions is the most effective way for improving the precision of posterior time estimation. However, even if a huge amount of sequence data is analyzed, considerable uncertainty will persist in time estimates.
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK.
authors.forename: Mario
authors.initials: M
authors.lastname: Dos Reis
authors.affiliation: Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China Department of Genetics, Evolution and Environment, University College London, Darwin Building, Gower Street, London WC1E 6BT, UK z.yang@ucl.ac.uk.
authors.forename: Ziheng
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D002965:Classification / Q000379:methods*; D003198:Computer Simulation; D005580:Fossils; D008957:Models, Genetic; D010802:Phylogeny; D011323:Primates / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods*; D013995:Time; D035501:Uncertainty
keywords: Bayesian inference; divergence time; finite-sites theory; infinite-sites theory; posterior variance; relaxed clock