title: Enhanced biosynthesis of O-desmethylangolensin from daidzein by a novel oxygen-tolerant cock intestinal bacterium in the presence of atmospheric oxygen.
abstract: OBJECTIVE
  To improve the oxygen-tolerant capability of a newly isolated anaerobic bacterium and to biosynthesize O-desmethylangolensin (O-Dma) from daidzein aerobically.
  
  
  RESULTS
  After a long-term domestication process, an oxygen-tolerant bacterium, which we named Aeroto-AUH-JLC108, was derived from the newly isolated obligate anaerobic bacterium Clostridium sp. AUH-JLC108. Strain Aeroto-AUH-JLC108 differed from the natively anaerobic wild-type strain AUH-JLC108 by various characteristics, including a change in bacterial shape, biochemical characteristics and 16S rRNA gene sequences. Both the growth speed and the maximal optical density (OD) value of strain Aeroto-AUH-JLC108 grown aerobically were significantly increased compared to that of the wild-type strain grown anaerobically. The maximal concentration of the substrate daidzein that the oxygen-tolerant strain Aeroto-AUH-JLC108 grown aerobically was able to convert efficiently was 2.0 mmol l(-1) and 0.6 mmol l(-1) for strain AUH-JLC108 that was grown anaerobically.
  
  
  CONCLUSIONS
  Strain Aeroto-AUH-JLC108 is a conditional oxygen-tolerant bacterium. The growth speed, bacterial growth mass and bioconversion capability of strain Aeroto-AUH-JLC108 grown aerobically was significantly increased compared to that of the wild-type strain AUH-JLC108 grown anaerobically.
  
  
  CONCLUSIONS
  Strain Aeroto-AUH-JLC108 is the first reported pure culture responsible for the formation of O-Dma from daidzein in the presence of atmospheric oxygen.
authors.affiliation: College of Life Sciences, Agricultural University of Hebei, Baoding, China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: X-L
authors.initials: XL
authors.lastname: Wang
authors.forename: B-H
authors.initials: BH
authors.lastname: Chen
authors.forename: Q-H
authors.initials: QH
authors.lastname: Hao
authors.forename: S-Y
authors.initials: SY
authors.lastname: Wang
mesh_terms: D000332:Aerobiosis; D000818:Animals; D003013:Clostridium / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D007422:Intestines / Q000382:microbiology; D007529:Isoflavones / Q000096:biosynthesis* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010100:Oxygen
chemical_list: D007529:Isoflavones; C004742:daidzein; D010100:Oxygen; C050052:O-desmethylangolensin
keywords: O-desmethylangolensin; aerobic biotransformation; bacterial isolation; isoflavone daidzein; oxygen-tolerant domestication