title: Multiplex PCR analysis of clusters of unexplained viral respiratory tract infection in Cambodia.
abstract: BACKGROUND
  Fevers of unknown origin constitute a substantial disease burden in Southeast Asia. In majority of the cases, the cause of acute febrile illness is not identified.
  
  
  METHODS
  We used MassTag PCR, a multiplex assay platform, to test for the presence of 15 viral respiratory agents from 85 patients with unexplained respiratory illness representing six disease clusters that occurred in Cambodia between 2009 and 2012.
  
  
  RESULTS
  We detected a virus in 37 (44%) of the cases. Human rhinovirus, the virus detected most frequently, was found in both children and adults. The viruses most frequently detected in children and adults, respectively, were respiratory syncytial virus and enterovirus 68. Sequence analysis indicated that two distinct clades of enterovirus 68 were circulating during this time period.
  
  
  CONCLUSIONS
  This is the first report of enterovirus 68 in Cambodia and contributes to the appreciation of this virus as an important respiratory pathogen.
authors.affiliation: U.S. Naval Medical Research Unit-2 Detachment, Phnom Penh, Cambodia. naryly@gmail.com.
authors.forename: Nary
authors.initials: N
authors.lastname: Ly
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA. rt2249@cumc.columbia.edu.
authors.forename: Rafal
authors.initials: R
authors.lastname: Tokarz
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA. nm2641@cumc.columbia.edu.
authors.forename: Nischay
authors.initials: N
authors.lastname: Mishra
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA. scs2178@columbia.edu.
authors.forename: Stephen
authors.initials: S
authors.lastname: Sameroff
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA. kj2230@cumc.columbia.edu.
authors.forename: Komal
authors.initials: K
authors.lastname: Jain
authors.affiliation: U.S. Naval Medical Research Unit-2 Detachment, Phnom Penh, Cambodia. Agus.Rachmat@namru2.org.kh.
authors.forename: Agus
authors.initials: A
authors.lastname: Rachmat
authors.affiliation: National Institute of Public Health, Cambodian Ministry of Health, Phnom Penh, Cambodia. usa@camnet.com.kh.
authors.forename: Ung Sam
authors.initials: US
authors.lastname: An
authors.affiliation: U.S. Naval Medical Research Unit-2 Detachment, Phnom Penh, Cambodia. Newell@med.navy.mil.
authors.forename: Steven
authors.initials: S
authors.lastname: Newell
authors.affiliation: U.S. Naval Medical Research Unit-2 Detachment, Phnom Penh, Cambodia. Dustin.Harrison@namru2.org.kh.
authors.forename: Dustin J
authors.initials: DJ
authors.lastname: Harrison
authors.affiliation: Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York City, USA. wil2001@columbia.edu.
authors.forename: W Ian
authors.initials: WI
authors.lastname: Lipkin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002160:Cambodia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D060885:Multiplex Polymerase Chain Reaction; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D017422:Sequence Analysis, DNA; D014777:Virus Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D014780:Viruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D055815:Young Adult