title: Methods for the successful crystallization of membrane proteins.
abstract: In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with structure determination of membrane proteins by X-ray crystallography. The advent of genomics and proteomics initiatives combined with high-throughput technologies, such as automation, miniaturization, integration, and third-generation synchrotrons, has enhanced membrane protein structure determination rate. Nevertheless, crystallization of membrane proteins still remains one of the most troublesome hurdles that every structural group must undertake. This chapter presents high-throughput methods easily available to any researcher interested in membrane protein characterization and crystallization. It is our hope this chapter can be used as a positive guide to all who are attempting crystallizing membrane proteins.
authors.affiliation: Department of Life Sciences, Imperial College London, London, SW7 2AZ, UK, i.moraes@imperial.ac.uk.
authors.forename: Isabel
authors.initials: I
authors.lastname: Moraes
authors.forename: Margarida
authors.initials: M
authors.lastname: Archer
mesh_terms: D003461:Crystallography / Q000295:instrumentation* / Q000379:methods*; D003902:Detergents / Q000737:chemistry; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods; D008565:Membrane Proteins / Q000737:chemistry*; D011487:Protein Conformation; D040901:Proteomics / Q000379:methods; D017356:Synchrotrons
chemical_list: D003902:Detergents; D008565:Membrane Proteins