title: [Effect of electroacupuncture intervention on neurological function, cerebral blood flow and cerebral cytochrome P 450 2 C 11 mRNA expression in rats with focal cerebral ischemia].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture(EA) intervention on changes of neurological function and expression of cerebral cytochrome P 450 2 C 11 (CYP 2 0 11) mRNA in focal cerebral ischemia (FCI) rats, so as to explore its mechanism underlying improvement of ischemic cerebral vascular disease.
  
  
  METHODS
  Wistar rats were randomly divided into normal, model, EA and EA+ 17-ODYA (17-Octadecynoic acid, an inhibitor for the metabolism of arachidonic acid by cytochrome P 450) groups. Focal cerebral ischemia rats were induced by middle cerebral artery occlusion (MCAO) with thread embolus. EA was applied to bilateral "Neiguan"(PC 6 ) and "Quchi" (LI 11) after MCAO. Zea Longa's score and beam walking test (BWT) score of rats were used to evaluate the neurological impairment. Local cerebral blood flow (LCBF) of the pial tissue was moni- tored using Laser-Doppler Flowmetry. The expression of cerebral CYP 2 C 11 mRNA was examined by Real-time Quantitative PCR (qPCR).
  
  
  RESULTS
  In comparison with the normal group, BWT score and LCBF of the model group were significantly decreased (P<0.05), and Zea Longa's score and cerebral CYP 2 C 11 mRNA expression level of the model group were significantly increased (P<0.05). While in comparison with the model group, BWT score, LCBF and cerebral CYP2 C 11 mRNA levels were considerably up-regulated and Zea Longa's score was down-regulated in the EA group (P<0.05) rather than in the EA + 17- ODYA group (P<0.05). H.E. stainshowed that the nerve impairment of the ischemic cerebral tissue including the neuronal degeneration, necrosis, apoptosis, etc. was relatively milder in the EA group.
  
  
  CONCLUSIONS
  EA intervention can improve cerebral blood flow and up-regulate cerebral CYP 2 C 11 mRNA expression in FCI rats, which may contribute to its action in improving neu- rological impairment.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.forename: Hai-jun
authors.initials: HJ
authors.lastname: Zhao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Bing-bing
authors.initials: BB
authors.lastname: Han
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.forename: Shi-jun
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000201:enzymology* / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D002560:Cerebrovascular Circulation; D003577:Cytochrome P-450 Enzyme System; D015671:Electroacupuncture; D015870:Gene Expression; D006801:Humans; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar
chemical_list: D003577:Cytochrome P-450 Enzyme System