title: Fibronectin immobilization on to robotic-dispensed nanobioactive glass/polycaprolactone scaffolds for bone tissue engineering.
abstract: Bioactive nanocomposite scaffolds with cell-adhesive surface have excellent bone regeneration capacities. Fibronectin (FN)-immobilized nanobioactive glass (nBG)/polycaprolactone (PCL) (FN-nBG/PCL) scaffolds with an open pore architecture were generated by a robotic-dispensing technique. The surface immobilization level of FN was significantly higher on the nBG/PCL scaffolds than on the PCL scaffolds, mainly due to the incorporated nBG that provided hydrophilic chemical-linking sites. FN-nBG/PCL scaffolds significantly improved cell responses, including initial anchorage and subsequent cell proliferation. Although further in-depth studies on cell differentiation and the in vivo animal responses are required, bioactive nanocomposite scaffolds with cell-favoring surface are considered to provide promising three-dimensional substrate for bone regeneration.
authors.affiliation: Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 330-714, South Korea.
authors.forename: Jong-Eun
authors.initials: JE
authors.lastname: Won
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Mateos-Timoneda
authors.forename: Oscar
authors.initials: O
authors.lastname: Castano
authors.forename: Josep A
authors.initials: JA
authors.lastname: Planell
authors.forename: Seog-Jin
authors.initials: SJ
authors.lastname: Seo
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.forename: Cheol-Min
authors.initials: CM
authors.lastname: Han
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D005353:Fibronectins / Q000378:metabolism*; D055767:Immobilized Proteins / Q000378:metabolism; D010011:Osteocytes / Q000502:physiology*; D011485:Protein Binding; D017207:Rats, Sprague-Dawley; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D005353:Fibronectins; D055767:Immobilized Proteins