title: Purification and characterisation of arabinoxylan arabinofuranohydrolase I responsible for the filterability of barley malt.
abstract: Dan'er is a widely grown malt barley cultivar in China, but its filterability defects have severely impeded its application in beer brewing. Previous investigations have suggested that we should identify the malt filterability correlated proteins, one of which was postulated to be arabinoxylan arabinofuranohydrolase I (AXAH-I). To verify this hypothesis, we purified AXAH-I from Dan'er malt, characterised its enzyme performance, and investigated its influence on filterability by adding different amounts of purified enzyme to the mash. With 6 mU g(-1) malt AXAH-I supplemented, the wort separation rate increased by 31.8%, viscosity decreased by 3.6%, and the endosperm reserve contents declined concomitantly. Unexpectedly, the wort turbidity increased with increasing AXAH-I. We also tried to optimise the use of currently available commercial enzyme products for filterability improvement in beer brewing, by supplementing them with purified AXAH-I and β-amylase. AXAH-I could be a functional component for novel commercial enzyme products in the beer industry.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Li
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Guolin
authors.initials: G
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jin
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China; School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, PR China. Electronic address: jlu@jiangnan.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co., Ltd, Qingdao 266100, PR China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co., Ltd, Qingdao 266100, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co., Ltd, Qingdao 266100, PR China.
authors.forename: Junhong
authors.initials: J
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co., Ltd, Qingdao 266100, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
mesh_terms: D001515:Beer / Q000032:analysis; D002681:China; D056625:Endosperm / Q000737:chemistry; D005374:Filtration; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D001467:Hordeum / Q000201:enzymology*
chemical_list: D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase
keywords: Arabinoxylan; Arabinoxylan arabinofuranohydrolase; Barley malt; Dan’er; Filterability