title: Effect of 1,3-propanediol, organic acids, and ethanol on growth and metabolism of Clostridium butyricum DSP1.
abstract: Knowledge of tolerance of bacteria to toxic stress is important, especially for processes targeted at high final titers of product. Information on environmental limits and stress responses may help during selection of strains or design and control of processes. The influence of the main product and its co-products on the process of 1,3-propanediol (PD) synthesis was determined. Adaptation to toxic compounds was noticed as Clostridium butyricum DSP1 was less sensitive to the addition of these factors during its exponential growth on glycerol than when the factor was present in the medium before inoculation. It was also shown that the response of the tested strain to the toxicity of 1,3-propanediol (1,3-PD) has different proteomic profiles depending on the stage of culture when this substance is introduced. Relatively satisfactory activity of the analyzed strain was sustained up to a concentration of 1,3-PD of 40 g/L while 80 g/L of this metabolite was lethal to the bacterium. As for the by-products, acetic acid was determined to be the most toxic among the acids excreted during the process.
authors.affiliation: Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-527, Poznan, Poland, daria.szymanowska@gmail.com.
authors.forename: Daria
authors.initials: D
authors.lastname: Szymanowska-Powałowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Kubiak
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D020148:Butyric Acid / Q000494:pharmacology; D046972:Clostridium butyricum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000494:pharmacology*; D005285:Fermentation; D019344:Lactic Acid / Q000494:pharmacology; D011409:Propylene Glycols / Q000378:metabolism / Q000494:pharmacology*; D013312:Stress, Physiological
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D011409:Propylene Glycols; D020148:Butyric Acid; D019344:Lactic Acid; D000431:Ethanol; C041787:1,3-propanediol; D019342:Acetic Acid