title: An Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes.
abstract: The life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.
authors.affiliation: Department of Molecular Microbiology and Biology of Infections and Service of Proteomics and Genomics, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas), Madrid, Spain.
authors.forename: Pedro J
authors.initials: PJ
authors.lastname: Alcolea
authors.affiliation: Department of Molecular Microbiology and Biology of Infections and Service of Proteomics and Genomics, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas), Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Alonso
authors.affiliation: Department of Molecular Microbiology and Biology of Infections and Service of Proteomics and Genomics, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas), Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: García-Tabares
authors.affiliation: Servicio de Inmunología, Centro Nacional de Microbiología, Virología e Inmunología Sanitarias (Instituto de Salud Carlos III), Majadahonda, Madrid, Spain.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Toraño
authors.affiliation: Department of Molecular Microbiology and Biology of Infections and Service of Proteomics and Genomics, Centro de Investigaciones Biológicas (Consejo Superior de Investigaciones Científicas), Madrid, Spain.
authors.forename: Vicente
authors.initials: V
authors.lastname: Larraga
mesh_terms: D000371:Agglutination; D061245:Axenic Culture; D016831:Crithidia fasciculata / Q000254:growth & development* / Q000378:metabolism*; D006706:Homeostasis; D007700:Kinetics; D007891:Leishmania / Q000254:growth & development*; D050356:Lipid Metabolism; D010084:Oxidation-Reduction; D019887:Peanut Agglutinin / Q000378:metabolism*; D010427:Pentose Phosphate Pathway; D040901:Proteomics; D015800:Protozoan Proteins / Q000378:metabolism; D015398:Signal Transduction; D013045:Species Specificity; D013438:Sulfhydryl Compounds / Q000378:metabolism; D059467:Transcriptome
chemical_list: D019887:Peanut Agglutinin; D015800:Protozoan Proteins; D013438:Sulfhydryl Compounds