title: Ursolic acid promotes apoptosis of SGC-7901 gastric cancer cells through ROCK/PTEN mediated mitochondrial translocation of cofilin-1.
abstract: Ursolic acid, extracted from the traditional Chinese medicine bearberry, can induce apoptosis of gastric cancer cells. However, its pro-apoptotic mechanism still needs further investigation. More and more evidence demonstrates that mitochondrial translocation of cofilin-1 appears necessary for the regulation of apoptosis. Here, we report that ursolic acid (UA) potently induces the apoptosis of gastric cancer SGC-7901 cells. Further mechanistic studies revealed that the ROCK1/PTEN signaling pathway plays a critical role in UA-mediated mitochondrial translocation of cofilin-1 and apoptosis. These findings imply that induction of apoptosis by ursolic acid stems primarily from the activation of ROCK1 and PTEN, resulting in the translocation of cofilin-1 from cytoplasm to mitochondria, release of cytochrome c, activation of caspase-3 and caspase-9, and finally inducing apoptosis of gastric cancer SGC-7901 cells.
authors.affiliation: Department of Clinical Laboratories, the Fifth People's Hospital of Shenyang and Tumor Hospital of Shenyang, Shenyang, China E-mail : lydsci2015@163.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Zhang
authors.forename: Hong-Hai
authors.initials: HH
authors.lastname: Chen
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Liu
mesh_terms: D000577:Amides / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D051338:Cofilin 1 / Q000378:metabolism*; D045304:Cytochromes c / Q000378:metabolism; D004789:Enzyme Activation; D006801:Humans; D008516:Medicine, Chinese Traditional; D008928:Mitochondria / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000188:drug therapy; D014315:Triterpenes / Q000494:pharmacology*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000097245:Ursolic Acid
chemical_list: D000577:Amides; D000972:Antineoplastic Agents, Phytogenic; D053148:Caspase 3; D053453:Caspase 9; D051338:Cofilin 1; D045304:Cytochromes c; D051059:PTEN Phosphohydrolase; D011725:Pyridines; D014315:Triterpenes; D054460:rho-Associated Kinases; D000097245:Ursolic Acid; C108830:Y 27632; C515851:ROCK1 protein, human; C494929:PTEN protein, human