title: Basophils are inept at promoting human Th17 responses.
abstract: Basophils are the rare granulocytes and play an important role in the polarization of Th2 responses and protection against helminth parasites. In addition, basophils contribute to the pathogenesis of several diseases such as asthma, chronic allergy and lupus. Notably, Th17 cells are also implicated in the pathogenesis of these diseases suggesting that basophils support the activation and expansion of this subset of CD4(+) T cells. Therefore, we explored whether basophils promote the expansion of human Th17 cells. We show that basophils lack the capacity to expand Th17 cells and to induce the secretion of Th17 cytokines either directly or indirectly via antigen presenting cells such as monocytes. As human basophils lack HLA-DR and co-stimulatory molecules, their inability to confer T cell receptor- and co-stimulatory molecule-mediated signals to CD4(+) T cells might explain the lack of Th17 responses when memory CD4(+) T cells were co-cultured with basophils.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 1138, Paris F-75006, France; Université de Technologie de Compiègne, Compiègne F-60205, France; Centre de Recherche des Cordeliers, Equipe - Immunopathology and Therapeutic Immunointervention, Paris F-75006, France.
authors.forename: Meenu
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 1138, Paris F-75006, France; Centre de Recherche des Cordeliers, Equipe - Immunopathology and Therapeutic Immunointervention, Paris F-75006, France; Sorbonne Universités, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Stephen-Victor
authors.affiliation: Armand Trousseau Children Hospital, Biochemistry Department, "Allergy & Environment" Group, Paris F-75012, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Poncet
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 1138, Paris F-75006, France; Centre de Recherche des Cordeliers, Equipe - Immunopathology and Therapeutic Immunointervention, Paris F-75006, France; Sorbonne Universités, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France; Université Paris Descartes, Sorbonne Paris Cité, UMR S 1138, Paris F-75006, France; International Associated Laboratory IMPACT (Institut National de la Santé et de la Recherche Médicale, France - Indian Council of Medical Research, India), National Institute of Immunohaematology, Mumbai 400012, India.
authors.forename: Srini V
authors.initials: SV
authors.lastname: Kaveri
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité 1138, Paris F-75006, France; Centre de Recherche des Cordeliers, Equipe - Immunopathology and Therapeutic Immunointervention, Paris F-75006, France; Sorbonne Universités, UPMC Univ Paris 06, UMR S 1138, Paris F-75006, France; Université Paris Descartes, Sorbonne Paris Cité, UMR S 1138, Paris F-75006, France; International Associated Laboratory IMPACT (Institut National de la Santé et de la Recherche Médicale, France - Indian Council of Medical Research, India), National Institute of Immunohaematology, Mumbai 400012, India. Electronic address: jagadeesh.bayry@crc.jussieu.fr.
authors.forename: Jagadeesh
authors.initials: J
authors.lastname: Bayry
mesh_terms: D017951:Antigen Presentation; D001491:Basophils / Q000276:immunology*; D015550:Cell Degranulation; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D016207:Cytokines / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D058504:Th17 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D006684:HLA-DR Antigens
keywords: Basophils; IL-17; IL-22; Monocytes; Th17