title: Cdc6 localizes to S- and G2-phase centrosomes in a cell cycle-dependent manner.
abstract: The Cdc6 protein has been primarily investigated as a component of the pre-replicative complex for the initiation of chromosome replication, which contributes to maintenance of chromosomal integrity. Here, we show that Cdc6 localized to the centrosomes during S and G2 phases of the cell cycle. The centrosomal localization was mediated by Cdc6 amino acid residues 311-366, which are conserved within other Cdc6 homologues and contains a putative nuclear export signal. Deletions or substitutions of the amino acid residues did not allow the proteins to localize to centrosomes. In contrast, DsRed tag fused to the amino acid residues localized to centrosomes. These results indicated that a centrosome localization signal is contained within amino acid residues 311-366. The cell cycle-dependent centrosomal localization of Cdc6 in S and G2 phases suggest a novel function of Cdc6 in centrosomes.
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Gwang Su
authors.initials: GS
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jeeheon
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sung Woong
authors.initials: SW
authors.lastname: Bang
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: dshwang@snu.ac.kr.
authors.forename: Deog Su
authors.initials: DS
authors.lastname: Hwang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism*; D016195:G2 Phase; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D021382:Protein Sorting Signals; D016196:S Phase; D017384:Sequence Deletion
chemical_list: C103706:CDC6 protein, human; D018797:Cell Cycle Proteins; D009687:Nuclear Proteins; D021382:Protein Sorting Signals
keywords: Cdc6; Cell cycle; Centrosome; DNA replication