title: Genetic identification of bucktooth parrotfish Sparisoma radians (Valenciennes, 1840) (Labridae, Scarinae) by chromosomal and molecular markers.
abstract: Parrotfishes (Labridae, Scarinae) comprise a large marine fish group of difficult identification, particularly during juvenile phase when the typical morphology and coloration of adults are absent. Therefore, the goal of this study was to test cytogenetic markers and DNA barcoding in the identification of bucktooth parrtotfish Sparisoma radians from the northeastern coast of Brazil. Sequencing of cytochrome c oxidase subunit I (COI) confirmed all studied samples as S. radians, and all showed high similarity (99-100%) with Caribbean populations. The karyotype of this species was divergent from most marine Perciformes, being composed of 2n = 46 chromosomes. These consisted of a large number of metacentric and submetacentric pairs with small amounts of heterochromatin and GC-rich single nucleolar organizer regions (NORs) not syntenic to 5S rDNA clusters. These are the first data about DNA barcoding in parrotfish from the Brazilian province and the first refined chromosomal analysis in Scarinae, providing useful data to a reliable genetic identification of S. radians.
authors.affiliation: Departamento de Ciências Biológicas , Universidade Estadual do Sudoeste da Bahia , Jequié, BA , Brazil .
authors.forename: Fabilene Gomes
authors.initials: FG
authors.lastname: Paim
authors.affiliation: Departamento de Ciências Biológicas , Universidade Estadual do Sudoeste da Bahia , Jequié, BA , Brazil .
authors.forename: José Henrique Souza Galdino
authors.initials: JH
authors.lastname: Brandão
authors.affiliation: Instituto de Estudos Costeiros , Universidade Federal do Pará , Bragança, PA , Brazil .
authors.forename: Iracilda
authors.initials: I
authors.lastname: Sampaio
authors.affiliation: Departamento de Ciências Biológicas , Universidade Estadual do Sudoeste da Bahia , Jequié, BA , Brazil .
authors.forename: Paulo Roberto Antunes
authors.initials: PR
authors.lastname: de Mello Affonso
authors.affiliation: Departamento de Ciências Biológicas , Universidade Estadual do Sudoeste da Bahia , Jequié, BA , Brazil .
authors.forename: Débora
authors.initials: D
authors.lastname: Diniz
keywords: COI; Robertsonian rearrangements; South Atlantic fish; karyotype; ribossomal genes