title: RORα inhibits adipocyte-conditioned medium-induced colorectal cancer cell proliferation and migration and chick embryo chorioallantoic membrane angiopoiesis.
abstract: Lipid metabolic disturbances are related to many diseases, such as obesity, diabetes, and certain cancers. Notably, lipid metabolic disturbances have been reported to be a risk factor for colorectal cancer. Nuclear receptors act as ligand-dependent transcription regulators and play key roles in the regulation of body lipid metabolism and the development of many cancers. Retinoic acid receptor-related orphan receptor α (RORα) is a nuclear receptor and can regulate several lipid metabolism genes in certain cancers. Herein, we demonstrate that the conditioned medium from adipocytes has a proproliferative and promigratory effect on colorectal cancer cells and enhances angiogenesis in chicken embryonic chorioallantoic membranes. In addition, the conditioned medium leads to a decrease in the expression of RORα and its target genes. Meanwhile, RORα and its target gene expressions are lower in human colorectal tumor tissue compared with control colorectal tissue. Activation of RORα inhibits the effect of conditioned medium on the proliferation and migration of colorectal cancer cells as well as the angiogenesis in chicken embryonic allantoic membranes. In colorectal cancer cells, the putative ligand of RORα, cholesterol sulfate (CS), prevents cell cycle progression at the G1/S boundary and concurrently modulates the expression of cell cycle-regulatory genes in colorectal cancer cell. CS inhibits angiogenesis in chicken embryonic chorioallantoic membranes and concurrently decreases the mRNA expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1α as well as the secretion of VEGF. In addition, lipogenic gene expression is higher in human colorectal tumor tissue compared with control colorectal tissue. CS inhibits the expression of lipogenic genes in colorectal cancer cells. These results suggest that RORα could represent a direct link between local lipid metabolism of colorectal tissue and colorectal cancer. Therefore, the reduction of the expression of RORα could represent a potential warning sign of colorectal cancer.
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China; Cardiovascular Research Center, School of Medicine, Xi'an Jiaotong University, Xi'an, China;
authors.forename: Lei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China;
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China;
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China;
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China;
authors.forename: Pengfei
authors.initials: P
authors.lastname: Xu
authors.affiliation: The Second Artillery General Hospital of Chinese People's Liberation Army, Beijing, China; and.
authors.forename: Suozhu
authors.initials: S
authors.lastname: Sun
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China;
authors.forename: Dacheng
authors.initials: D
authors.lastname: He
authors.affiliation: Institute of Aging Research, Hangzhou Normal University, Hangzhou, China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Cong
authors.affiliation: Beijing Key Laboratory of Gene Resource and Molecular Development and College of Life Sciences, Beijing Normal University, Beijing, People's Republic of China; ygzhai@bnu.edu.cn.
authors.forename: Yonggong
authors.initials: Y
authors.lastname: Zhai
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000502:physiology; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply / Q000187:drug effects / Q000502:physiology*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
chemical_list: D017077:Culture Media, Conditioned; D057094:Nuclear Receptor Subfamily 1, Group F, Member 1; C539738:RORA protein, human
keywords: colorectal cancer; lipid metabolism; retinoic acid receptor-related orphan receptor α