title: Evaluation of cytotoxicity and genotoxicity of Acacia aroma leaf extracts.
abstract: Acacia aroma, native plant from San Luis, Argentina, is commonly used as antiseptic and for healing of wounds. The present study was conducted to investigate the in vitro cytotoxicity and genotoxicity of hot aqueous extract (HAE) and ethanolic extract (EE) of A. aroma. The cytotoxic activity was assayed by neutral red uptake assay on Vero cell. Cell treatment with a range from 100 to 5000 μg/mL of HAE and EE showed that 500 μg/mL and 100 μg/mL were the maximum noncytotoxic concentrations, respectively. The CC50 was 658 μg/mL for EE and 1020 μg/mL for HAE. The genotoxicity was tested by the single-cell gel electrophoresis comet assay. The results obtained in the evaluation of DNA cellular damage exposed to varied concentrations of the HAE showed no significant genotoxic effect at range of 1-20 mg/mL. The EE at 20 mg/mL showed moderate genotoxic effect related to the increase of the DNA percentage contained in tail of the comet; DNA was classified in category 2. At concentrations below 5 mg/mL, the results of cytotoxicity and genotoxicity of aqueous and ethanolic extracts of Acacia aroma guarantee the safety at cell and genomic level. However further studies are needed for longer periods including animal models to confirm the findings.
authors.affiliation: Área de Microbiología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina.
authors.forename: C M
authors.initials: CM
authors.lastname: Mattana
authors.affiliation: Área de Biología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Cangiano
authors.affiliation: Área de Microbiología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina.
authors.forename: L E
authors.initials: LE
authors.lastname: Alcaráz
authors.affiliation: Área de Farmacognosia, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Sosa
authors.affiliation: Departamento de Microbiología e Inmunología, Fac de Cs Ex. Fco-Qco y Nat., UNRC, Río Cuarto, Córdoba, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Escobar
authors.affiliation: Departamento de Microbiología e Inmunología, Fac de Cs Ex. Fco-Qco y Nat., UNRC, Río Cuarto, Córdoba, Argentina.
authors.forename: C
authors.initials: C
authors.lastname: Sabini
authors.affiliation: Departamento de Microbiología e Inmunología, Fac de Cs Ex. Fco-Qco y Nat., UNRC, Río Cuarto, Córdoba, Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Sabini
authors.affiliation: Área de Microbiología, Fac de Qca, Bqca y Fcia, UNSL, San Luis, Argentina.
authors.forename: A L
authors.initials: AL
authors.lastname: Laciar
mesh_terms: D000045:Acacia / Q000737:chemistry*; D000328:Adult; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004249:DNA Damage; D006801:Humans; D009153:Mutagens / Q000633:toxicity*; D010936:Plant Extracts / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D009153:Mutagens; D010936:Plant Extracts