title: Network-based gene expression analysis of vascular wall of juvenile Moyamoya disease.
abstract: METHODS
  Little is known about the pathology and pathogenesis of the Moyamoya disease (MMD). To better understand the molecular processes involved in MMD gene expression analysis of Moyamoya artery tissue to a control artery, this study was conducted.
  
  
  METHODS
  Tissue samples of two MMD and two non-MMD were profiled using oligonucleotide microarrays. Gene ontology classification of the differentially expressed genes was analyzed, and regulatory functional networks and pathways were identified with a network-based computational pathway analysis tool. Analysis of MMD and control tissue revealed 104 differentially expressed genes.
  
  
  RESULTS
  The two major significantly associated gene ontology terms was cellular development and cellular movement. Further network-based analysis showed significant interaction between RNF213 downstream gene networks and the top 3 score gene networks of MMD. Three major nodes of this network were evident in the merged network and were showing interactions with downstream network of RNF213.
  
  
  CONCLUSIONS
  Our results demonstrate that cellular development and cellular movement in MMD are the key role of mechanisms.
authors.affiliation: Department of Neurosurgery, Tokyo Women's Medical University, 8-1 Kawada-cho Shinjuku-ku, Tokyo, 162-8666, Japan.
authors.forename: Nobuya
authors.initials: N
authors.lastname: Okami
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Aihara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Akagawa
authors.forename: Koji
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Akitsugu
authors.initials: A
authors.lastname: Kawashima
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000293:Adolescent; D002533:Cerebral Angiography; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D063990:Gene Ontology; D053263:Gene Regulatory Networks / Q000235:genetics*; D006801:Humans; D008297:Male; D009072:Moyamoya Disease / Q000235:genetics / Q000503:physiopathology*; D020411:Oligonucleotide Array Sequence Analysis