title: [Roles of CD4+CD25+Foxp3+ regulatory T cells and IL-33 in the pathogenesis of asthma in children].
abstract: OBJECTIVE
  To study the roles of CD4(+)CD25(+)Foxp3(+) regulatory T cells (Treg) and IL-33 in the pathogenesis of asthma in children.
  
  
  METHODS
  Flow cytometry was used to detect peripheral blood CD4(+)CD25(+)Foxp3(+)Treg proportion in CD4(+)T lymphocytes in.45 children with asthma, 50 children with wheezing caused by respiratory syncytial virus infection and 40 healthy children. Serum levels of IFN-γ, IL-4, IL-5 and IL-33 were measured using ELISA.
  
  
  RESULTS
  The level of peripheral blood CD4(+)CD25(+)Foxp3(+)Treg in the asthma group was significantly lower than in the wheezing and control groups (P<0.05). In contrast, serum levels of IL-33 in the asthma group was significantly higher than in the wheezing and control groups (P<0.05). Peripheral blood CD4(+)CD25(+)Foxp3(+)Treg level was negatively correlated with serum IL-33 level in the asthma group(r=-0.156, P<0.01).
  
  
  CONCLUSIONS
  CD4(+)CD25(+)Foxp3(+)Treg may interact with IL-33 in the pathogenesis of childhood asthma.
authors.affiliation: Department of Respiratory, Wuxi Children's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214023, China. sy3334@sina.com.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Pan
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: He
mesh_terms: D001249:Asthma / Q000209:etiology* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis*; D006801:Humans; D007223:Infant; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D000067596:Interleukin-33; D007378:Interleukins / Q000502:physiology*; D008297:Male; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508017:IL2RA protein, human; C506536:IL33 protein, human; D053645:Interleukin-2 Receptor alpha Subunit; D000067596:Interleukin-33; D007378:Interleukins