title: Comparative study of the bioconversion process using R-(+)- and S-(-)-limonene as substrates for Fusarium oxysporum 152B.
abstract: This study compared the bioconversion process of S-(-)-limonene into limonene-1,2-diol with the already established biotransformation of R-(+)-limonene into α-terpineol using the same biocatalyst in both processes, Fusarium oxysporum 152B. The bioconversion of the S-(-)-isomer was tested on cell permeabilisation under anaerobic conditions and using a biphasic system. When submitted to permeabilisation trials, this biocatalyst has shown a relatively high resistance; still, no production of limonene-1,2-diol and a loss of activity of the biocatalyst were observed after intense cell treatment, indicating a complete loss of cell viability. Furthermore, the results showed that this process can be characterised as an aerobic system that was catalysed by limonene-1,2-epoxide hydrolase, had an intracellular nature and was cofactor-dependent because the final product was not detected by an anaerobic process. Finally, this is the first report to characterise the bioconversion of R-(+)- and S-(-)-limonene by cellular detoxification using ultra-structural analysis.
authors.affiliation: Laboratory of Bioflavors, Department of Food Science, School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil; Institute of Science and Technology, Food Engineering, UFVJM, Diamantina, Minas Gerais, Brazil. Electronic address: gustavomolinagm@gmail.com.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Molina
authors.affiliation: Laboratory of Bioflavors, Department of Food Science, School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Murillo L
authors.initials: ML
authors.lastname: Bution
authors.affiliation: Laboratory of Bioflavors, Department of Food Science, School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Juliano L
authors.initials: JL
authors.lastname: Bicas
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Mary Anne Heidi
authors.initials: MA
authors.lastname: Dolder
authors.affiliation: Laboratory of Bioflavors, Department of Food Science, School of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil.
authors.forename: Gláucia M
authors.initials: GM
authors.lastname: Pastore
mesh_terms: D001711:Biotransformation / Q000502:physiology*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry*; D005670:Fusarium / Q000737:chemistry*; D000077222:Limonene; D039821:Monoterpenes / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry*
chemical_list: D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D013729:Terpenes; C016775:alpha-terpineol; D000077222:Limonene
keywords: Anaerobic biotransformation; Cell permeabilisation; Limonene-1,2-diol; Monoterpenes; α-Terpineol