title: A low-toxic artificial fluorescent glycoprotein can serve as an efficient cytoplasmic labeling in living cell.
abstract: To maintain the virtue of good optical property and discard the dross of conventional fluorescent staining dyes, we provide a strategy for designing new fluorescent scaffolds. In this study, a novel fluorescent labeling glycoprotein (chitosan-poly-L-cysteine, CPC) was synthesized through graft copolymerization. CPC gives emission peak at 465-470 nm when excited at 386 nm. The submicro-scale CPC microspheres could be localized and persisted specifically in the cytoplasm of living cells, with strong blue fluorescence. Moreover, CPC was highly resistant to photo bleaching, the fluorescence was remained stable for up to 72 h as the cells grew and developed. The glycoprotein CPC was bio-compatible and in zero grade cytotoxicity as quantified by MTT assay. The fluorescent labeling process with our newly designed glycoprotein CPC is exceptionally efficient.
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China; Beijing Key Laboratory of Bio-Inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China.
authors.forename: Jiangju
authors.initials: J
authors.lastname: Si
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China; Beijing Key Laboratory of Bio-Inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Liang
authors.affiliation: School of Public Health, Peking University Health Science Center, Beijing 100191, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Kong
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China; Beijing Key Laboratory of Bio-Inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Wu
authors.affiliation: Medical & Healthy Analysis Center, Peking University, Beijing 100191, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China; Beijing Key Laboratory of Bio-Inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China. Electronic address: xiangy@buaa.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China; Beijing Key Laboratory of Bio-Inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang University, Beijing 100191, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D003545:Cysteine / Q000737:chemistry; D003593:Cytoplasm / Q000187:drug effects; D005456:Fluorescent Dyes / Q000009:adverse effects / Q000138:chemical synthesis* / Q000494:pharmacology; D006023:Glycoproteins / Q000009:adverse effects / Q000138:chemical synthesis* / Q000494:pharmacology; D008164:Luminescent Proteins / Q000009:adverse effects / Q000138:chemical synthesis* / Q000502:physiology; D008863:Microspheres
chemical_list: D005456:Fluorescent Dyes; D006023:Glycoproteins; D008164:Luminescent Proteins; D048271:Chitosan; D003545:Cysteine
keywords: Artificial fluorescent glycoprotein; Chitosan-poly-l-cysteine; Cytoplasmic labeling; Thiol group