title: In-house preparation and evaluation of (125)I-histamine progesterone tracer for radioimmunoassay of progesterone.
abstract: The progesterone tracer for the development of in-house radioimmunoassay was prepared in two steps clearly described. First, activation of 11α-hemisuccinate progesterone (progesterone-11 α HS) and labeling of histamine with (125)I, and second, conjugation of activated 11α-HS progesterone with iodinated histamine. The purification of the tracer was carried out by gel filtration on Sephadex G-25. The radiochemical purity of purified the tracer was 95%. The maximum binding using solid phase (coated tubes) reached 41% and the non specific binding didn't exceed 5%. The tracer stored at different temperatures 10°C and -6°C was stable during 12 weeks. The skim milk provided assay better sensitivity (0.22 ng/mL) than serum (0.28 ng/mL).
authors.affiliation: a Nuclear Research Center of Draria (CRND) , Algiers , Algeria.
authors.forename: A
authors.initials: A
authors.lastname: Benabdelaziz
authors.forename: S
authors.initials: S
authors.lastname: Boudjemai
authors.forename: A
authors.initials: A
authors.lastname: Benzaid
authors.forename: R
authors.initials: R
authors.lastname: Khelili
authors.forename: R
authors.initials: R
authors.lastname: Kaidi
mesh_terms: D005260:Female; D006632:Histamine / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes / Q000378:metabolism*; D008895:Milk, Human / Q000378:metabolism; D011374:Progesterone / Q000097:blood / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D007457:Iodine Radioisotopes; D011374:Progesterone; D006632:Histamine
keywords: early pregnancy diagnostic; iodohistamine; progesterone RIA; progesterone cow; steroid radioiodination