title: Factors influencing production of lipase under metal supplementation by bacterial strain, Bacillus subtilis BDG-8.
abstract: Lipases are biocatalyst having wide applications in industries due to their versatile properties. In the present study, a lipolytic bacterial strain, Bacillus subtilis BDG-8 was isolated from an oil based industrial soil. The effect of selenium and nickel as a media supplement on enhancement of lipase production, was studied individually with the isolated strain by varying the concentration of selected metal. 60 μg l(-1) selenium enhanced lipase production to an enzyme activity measuring 7.8 U ml(-1) while 40 μgI(-1) nickel gave the maximum enzyme activity equivalent to 7.5 U ml(-1). However, nickel and selenium together at a range of concentration with an equal w/v ratio, at 60 μg l(-1) each, showed the maximum lipase activity of 8.5 U ml(-1). The effect of pH and temperature on lipase production showed maximum enzyme activity in the presence of each of the metals at pH 7 and 35°C among the other tested ranges. After optimisation of the parameters such as metal concentration, pH and temperature lipase production by Bacillus subtilis BDG-8 had increased several folds. This preliminary investigation may consequently lead as to various industrial applications such as treatment of wastewater contaminated with metal or oil with simultaneous lipase production.
authors.forename: B
authors.initials: B
authors.lastname: Dhevahi
authors.forename: R
authors.initials: R
authors.lastname: Gurusamy
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D008049:Lipase / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology*; D012643:Selenium / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D008670:Metals; D009532:Nickel; D008049:Lipase; D012643:Selenium