title: Molecular level evaluation on HEMA interaction with a collagen model.
abstract: OBJECTIVE
  2-Hydroxyethylmethacrylate (HEMA) diffuses in wet dentin and promotes adhesion during dentin priming and bonding. We have investigated the molecular level interaction between HEMA and a collagen model by using saturation transfer difference (STD) NMR.
  
  
  METHODS
  The binding of HEMA to collagen was preliminarily investigated by suspending demineralized human dentin powders in a 4mM HEMA solution for 1h and measuring the decrease in the HEMA concentration on a spectrophotometer. The molecular level interaction of HEMA with atelocollagen, which was used as a collagen model, was investigated by STD-NMR spectroscopy.
  
  
  RESULTS
  The HEMA concentration in the suspension did not change, indicating that HEMA did not bind to dentin collagen. This was confirmed by STD-NMR; when the atelocollagen resonance was saturated, no saturation was propagated to HEMA and no STD signals were detected.
  
  
  CONCLUSIONS
  The HEMA protons were not near the atelocollagen surface, indicating HEMA did not interact with atelocollagen. The collagen fibrils may be surrounded by water molecules in dentin/bond interfaces, which prevent the direct HEMA binding interaction.
authors.affiliation: Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan. Electronic address: hiraope@tmd.ac.jp.
authors.forename: Noriko
authors.initials: N
authors.lastname: Hiraishi
authors.affiliation: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima 739-8511, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Tochio
authors.affiliation: NMR Pipeline Methodology Research Team, RIKEN Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Kigawa
authors.affiliation: Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Otsuki
authors.affiliation: Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases at Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Tagami
mesh_terms: D003094:Collagen / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008689:Methacrylates / Q000737:chemistry*; D008963:Molar
chemical_list: D017220:Dentin-Bonding Agents; D008689:Methacrylates; C059821:atelocollagen; C005044:hydroxyethyl methacrylate; D003094:Collagen
keywords: 2-ydroxyethylmethacrylate; Atelocollagen; Dental adhesives; Dentin; Nuclear magnetic resonance