title: Phosphatidylcholine bearing 6,6-dideuterated oleic acid: a useful solid-state (2)H NMR probe for investigating membrane properties.
abstract: Lipid organization has been at the center of research on lipid rafts. Dioleoylphosphatidylcholine (DOPC) is a typical unsaturated lipid. Very few studies have reported its thermodynamics in raft-like membranes. Herein, we have developed a highly efficient synthetic method for [C6-(2)H2] oleic acid, and newly synthesized [C6-(2)H2] DOPC. In raft-like oriented bilayers, [C6-(2)H2] DOPC shows clear phase separation and characteristic phase behavior at various temperature. It has been successfully utilized for the comparison of membrane properties between sphingomyelin (SM) and dihydrosphingomyelin (DHSM) membranes.
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Cui
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Lethu
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Yasuda
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Matsuoka
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Matsumori
authors.affiliation: JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Sato
authors.affiliation: Department of Chemistry, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; JST, ERATO, Lipid Active Structure Project, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan; Project Research Centre for Fundamental Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. Electronic address: murata@chem.sci.osaka-u.ac.jp.
authors.forename: Michio
authors.initials: M
authors.lastname: Murata
mesh_terms: D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D021962:Membrane Microdomains / Q000737:chemistry*; D019301:Oleic Acid / Q000032:analysis / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000032:analysis / Q000737:chemistry*
chemical_list: D010713:Phosphatidylcholines; D019301:Oleic Acid
keywords: Lipid raft; Phase separation; Phosphatidylcholine; Solid-state (2)H NMR