title: A suite of microsatellite markers for genetic management of captive cracids (Aves, Galliformes).
abstract: Cracids are medium to large frugivorous birds that are endemic to the Neotropics. Because of deforestation and overhunting, many species are threatened. The conservation of several species has relied on captive breeding and reintroduction in the wild, but captive populations may be inbred. Microsatellite tools can permit the construction of genetic pedigrees to reduce inbreeding, but only a few loci are available for this group of birds. Here, we present 10 novel polymorphic microsatellite loci and the cross-amplification of these and of 10 additional loci available in the literature in a panel of 5 cracid species, including 3 species with high conservation concern. We provide the first polymorphic loci for the jacutinga, Aburria jacutinga (N = 8), and red-billed curassow, Crax blumenbachii (N = 9), and additional loci for bare-faced curassow, C. fasciolata (N = 8), Alagoas curassow, Pauxi mitu (N = 5), and razor-billed curassow, P. tuberosa (N = 5). The average number of alleles was 2.9 for A. jacutinga, 2.7 for C. blumenbachii, 3.5 for C. fasciolata, 2.6 for P. mitu, and 5.7 for P. tuberosa. The mean expected heterozygosities were 0.42, 0.40, 0.48, 0.37, and 0.59, respectively. The average probabilities that the set of loci would not exclude a pair of parents of an arbitrary offspring were 2.9% in A. jacutinga, 1% in C. blumenbachii, 0.5% in C. fasciolata, 0.4% in P. mitu, and 0.002% in P. tuberosa suggesting that these loci may be adequate for parentage analysis and to implement ex situ genetic management plans.
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: M C
authors.initials: MC
authors.lastname: Costa
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: C
authors.initials: C
authors.lastname: Camargo
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: N M
authors.initials: NM
authors.lastname: Laganaro
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: P R R
authors.initials: PR
authors.lastname: Oliveira
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: P V
authors.initials: PV
authors.lastname: Davanço
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil.
authors.forename: R M A
authors.initials: RM
authors.lastname: Azeredo
authors.affiliation: CRAX, Sociedade de Pesquisa do Manejo e da Reprodução da Fauna Silvestre, Contagem, MG, Brasil.
authors.forename: J G P
authors.initials: JG
authors.lastname: Simpson
authors.affiliation: Seção de Aves, Museu de Zoologia da Universidade de São Paulo, São Paulo, SP, Brasil.
authors.forename: L F
authors.initials: LF
authors.lastname: Silveira
authors.affiliation: Departamento de Ciências Ambientais, Universidade Federal de São Carlos, Campus de Sorocaba, Sorocaba, SP, Brasil mercival@ufscar.br.
authors.forename: M R
authors.initials: MR
authors.lastname: Francisco
mesh_terms: D000483:Alleles; D000818:Animals; D003247:Conservation of Natural Resources; D046094:Galliformes / Q000235:genetics*; D056426:Genetic Loci; D006579:Heterozygote; D018895:Microsatellite Repeats / Q000235:genetics*