title: Mutagenic and antimutagenic effects of aqueous extract of rosemary (Rosmarinus officinalis L.) on meristematic cells of Allium cepa.
abstract: Polyphenolic compounds present in rosemary were found to have antioxidant properties, anticarcinogenic activity, and to increase the detoxification of pro-carcinogens. The aim of the study was to determine the effect the aqueous extract of rosemary (AER) on mutagenicity induced by methylmethane sulfonate in meristematic cells of Allium cepa, as well as to describe its mode of action. Anti-mutagenicity experiments were carried out with 3 different concentrations of AER, which alone showed no mutagenic effects. In antimutagenicity experiments, AER showed chemopreventive activity in cultured meristematic cells of A. cepa against exposure to methylmethane sulfonate. Additionally, post-treatment and simultaneous treatment using pre-incubation protocols were the most effective. Evaluation of different protocols and the percent reduction in DNA indicated bioantimutagenic as well desmutagenic modes of action for AER. AER may be chemopreventive and antimutagenic.
authors.affiliation: Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia, Londrina, PR, Brasil.
authors.forename: I
authors.initials: I
authors.lastname: Felicidade
authors.affiliation: Centro de Estudos em Nutrição e Genética Toxicológica, Centro Universitário Filadélfia, Londrina, PR, Brasil.
authors.forename: J D
authors.initials: JD
authors.lastname: Lima
authors.affiliation: Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: J R
authors.initials: JR
authors.lastname: Pesarini
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil.
authors.forename: A C D
authors.initials: AC
authors.lastname: Monreal
authors.affiliation: Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: M S
authors.initials: MS
authors.lastname: Mantovani
authors.affiliation: Programa de Pós-Graduação em Patologia, Faculdade de Medicina, Universidade Estadual Paulista, Botucatu, SP, Brasil.
authors.forename: L R
authors.initials: LR
authors.lastname: Ribeiro
authors.affiliation: Centro de Estudos em Célula Tronco, Terapia Celular e Genética Toxicológica, Núcleo de Hospital Universitário, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brasil rodrigo.oliveira@ufms.br.
authors.forename: R J
authors.initials: RJ
authors.lastname: Oliveira
mesh_terms: D016587:Antimutagenic Agents / Q000494:pharmacology*; D002869:Chromosome Aberrations; D004249:DNA Damage; D018519:Meristem / Q000166:cytology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008940:Mitotic Index; D009153:Mutagens / Q000494:pharmacology*; D019697:Onions / Q000166:cytology*; D010936:Plant Extracts / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D014867:Water; D008741:Methyl Methanesulfonate