title: Analytical characterization of label-free immunosensor subsystems based on multi-walled carbon nanotube array-modified gold interface.
abstract: In the present work, we report characterization studies of antigen(Ag)-antibody (Ab) interaction based on the multi-walled carbon nanotube array-modified gold electrode for label-free electrochemical immunosensor. -COOH functionalization of MWCNT was suggested by FTIR spectroscopy. Images from atomic force microscopy (AFM) and RAMAN spectroscopy confirmed the interaction of Ab on the gold interface based on the MWCNT array. A cyclic voltammetric study exhibited a linear response in phosphate buffered saline (PBS) solution including 1mM K3Fe (CN) 6 at pH 7.4 with PSA concentrations over a range of 2 to 40 ngmL(-1), and a calculated detection limit of 0.56 ngmL(-1).
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Zahmatkeshan
authors.forename: Hoda
authors.initials: H
authors.lastname: Ilkhani
authors.forename: Malihe
authors.initials: M
authors.lastname: Paknejad
authors.forename: Moein
authors.initials: M
authors.lastname: Adel
authors.forename: Saeed
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran. rsaber@sina.tums.ac.ir.
authors.forename: Reza
authors.initials: R
authors.lastname: Saber
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D051379:Mice; D037742:Nanotubes, Carbon / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis*; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013499:Surface Properties
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D006046:Gold; D017430:Prostate-Specific Antigen