title: Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: utility of 100 percent inhibition as the endpoint.
abstract: Four laboratories tested three isolates of Candida species and two isolates of Aspergillus fumigatus using 96-well plates containing combinations of amphotericin B, anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole, and voriconazole. The majority of summation fractional inhibitory concentration indices (ΣFICI) based on the Lowe additivity formula suggested indifferent drug interactions (ΣFICI > 0.5 and ≤4.0) and no instance of drug antagonism (ΣFICI > 4.0). The intra- and interlaboratory agreement rates were superior when MIC100 readings were used as endpoints (at a 99% confidence interval [CI]).
authors.affiliation: Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA ping.ren@health.ny.gov.
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.affiliation: Bureau of Environmental & Occupational Epidemiology, New York State Department of Health, Albany, New York, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.affiliation: Bureau of Environmental & Occupational Epidemiology, New York State Department of Health, Albany, New York, USA.
authors.forename: Shao
authors.initials: S
authors.lastname: Lin
authors.affiliation: Center for Medical Mycology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: Ghannoum
authors.affiliation: Center for Medical Mycology, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Isham
authors.affiliation: Medical Microbiology Division, Department of Pathology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Dan J
authors.initials: DJ
authors.lastname: Diekema
authors.affiliation: Medical Microbiology Division, Department of Pathology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Pfaller
authors.affiliation: Medical Microbiology Division, Department of Pathology, University of Iowa, Iowa City, Iowa, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Messer
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Shawn R
authors.initials: SR
authors.lastname: Lockhart
authors.affiliation: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
authors.forename: Naureen
authors.initials: N
authors.lastname: Iqbal
authors.affiliation: Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York, USA.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Chaturvedi
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects*; D002175:Candida / Q000187:drug effects*; D004338:Drug Combinations; D008826:Microbial Sensitivity Tests / Q000379:methods
chemical_list: D000935:Antifungal Agents; D004338:Drug Combinations