title: Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces.
abstract: This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast-like (osteo-1) cells cultured on chemically modified titanium surfaces. Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid-etched) titanium surfaces in 3 different types of medium: control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. The following parameters were evaluated: cell adhesion after 24 hours; total protein content; collagen content and alkaline phosphatase (AP) activity after 7, 14, and 21 days; and calcified nodule formation after 21 days. The addition of rhBMP-2 or rhBMP-7 did not influence cell adhesion (P = .1175). Cell differentiation was influenced by rhBMP-2, as demonstrated by a significant increase in collagen content after 7 days of culture (P < .0001) and a significant decrease in AP activity after 21 days (P < .0001). The addition of rhBMP-7 only influenced AP activity, and a significant increase was observed after 21 days (P < .0001). Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface.
authors.affiliation: 1  Paulista University, Department of Periodontology, São Paulo, Brazil.
authors.forename: Fabiano Ribeiro
authors.initials: FR
authors.lastname: Cirano
authors.forename: Adriane Yaeko
authors.initials: AY
authors.lastname: Togashi
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
authors.forename: Francisco Emílio
authors.initials: FE
authors.lastname: Pustiglioni
authors.forename: Luiz Antônio Pugliesi Alves
authors.initials: LA
authors.lastname: Lima
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D055419:Bone Morphogenetic Protein 7 / Q000494:pharmacology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000032:analysis; D004396:Coloring Agents; D045662:Dental Etching / Q000379:methods; D003764:Dental Materials / Q000737:chemistry*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D000880:Anthraquinones; C525819:BMP7 protein, human; D055396:Bone Morphogenetic Protein 2; D055419:Bone Morphogenetic Protein 7; D004396:Coloring Agents; D003764:Dental Materials; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C010078:alizarin; D003094:Collagen; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: bone morphogenetic protein; implants; osteoblasts; titanium surface