title: [Design of cell line stable expressing proteasomal subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag based on HEK293 cells].
abstract: A stable cell line based on HEK293 cells that expresses proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag has been selected. This chimera was shown to be incorporated completely into proteolitic active proteasomes. The created cell line can be used for further fluorescent studies of proteasomes localization in the cell.
authors.forename: V A
authors.initials: VA
authors.lastname: Kulichkova
authors.forename: Iu Ia
authors.initials: IuIa
authors.lastname: Zaĭkova
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Ermolaeva
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradova
authors.forename: L
authors.initials: L
authors.lastname: Huang
authors.forename: A N
authors.initials: AN
authors.lastname: Tomilin
authors.forename: N A
authors.initials: NA
authors.lastname: Barlev
authors.forename: A S
authors.initials: AS
authors.lastname: Tsimokha
mesh_terms: D060846:Cell Engineering; D018703:Founder Effect; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D013194:Staining and Labeling; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: C109285:PSMD14 protein, human; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D046988:Proteasome Endopeptidase Complex