title: Development of liposomal Ganoderma lucidum polysaccharide: formulation optimization and evaluation of its immunological activity.
abstract: The aim of this study was to investigate the optimizing preparation conditions of Ganoderma lucidum polysaccharide liposome (GLPL) with response surface methodology (RSM) and the immunological enhancement activity of GLPL. The immunological enhancement activity of GLPL on splenocyte proliferation was measured. The optimum formulation of GLPL, in which the ratio of soybean phospholipid to cholesterol(w/w) of 11:1, the ratio of soybean phospholipid to tween-80 (w/w) of 10.5:1 and ultrasonic time(min) of 11, had higher entrapment efficiency (EE) of 71.43±0.49% with spherical shape and uniform sizes. In addition, GLPL could significantly promote splenocyte proliferation singly or synergistically with PHA and LPS. That indicated that the immunological enhancement of Ganoderma lucidum polysaccharide (GLP) was significantly enhanced after encapsulation with the liposome.
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhenguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Henan University of Animal Husbandry & Economy, Zhengzhou 450011, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ma
authors.affiliation: National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Science, Nanjing 210014, China.
authors.forename: Bihua
authors.initials: B
authors.lastname: Deng
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ruonan
authors.initials: R
authors.lastname: Bo
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: dywang@njau.edu.cn.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D008081:Liposomes / Q000737:chemistry*; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology*; D020940:Reishi / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008081:Liposomes; D011134:Polysaccharides
keywords: Ganoderma lucidum polysaccharides liposome; Lymphocyte proliferation; Optimization; Response surface methodology