title: Low pericyte coverage of endometrial microvessels in heavy menstrual bleeding correlates with the microvessel expression of VEGF-A.
abstract: A prospective clinical study was carried out to investigate whether endometrial microvessels in patients with idiopathic heavy menstrual bleeding (HMB) of endometrial origin (HMB-E) are fragile due to low pericyte coverage. Idiopathic HMB-E is characterized by large endothelial cell gaps related to the microvascular overexpression of vascular endothelial growth factor (VEGF)-A and VEGF receptors 1-3. A total of 10 women with a normal menstrual cycle and a history of HMB of <5 years, and 17 healthy women with a normal menstrual cycle were recruited from the Karolinska University Hospital. Blood samples were obtained for hormone analysis and coagulation tests. Endometrial biopsies were collected in the proliferative or in the secretory phase. Pericyte coverage was assessed using immunohistochemical staining for smooth muscle actin-α (SMAα) and by image analysis (microvascular density) of endometrial biopsies from 10 patients with HMB-E and 17 healthy ovulating women (control subjects). Previously published data on endothelial cell gap size and the expression of VEGF receptors were used. Although microvascular density did not differ between the patients with HMB-E and the control subjects, the number of SMAα-positive microvessels in the proliferative phase was significantly (P=0.005) lower in the patients with HMB-E than in the control subjects. Moreover, the number of SMAα-positive microvessels in the control subjects was significantly fewer in the secretory (P=0.04) than in the proliferative phase, whereas this number did not differ among the patients with HMB-E regardless of phase. A significant negative correlation was observed between the number of VEGF-A-positive microvessels and microvessels with pericyte coverage (r=0.8; P=0.04). Finally, the endothelial cell layer was significantly thicker in the patients with HMB-E than in the control subjects. Thus, the upregulation of VEGF-A in idiopathic HMB-E is associated with a low pericyte coverage during the proliferative phase of intense angiogenesis, which may confer vessel fragility, possibly leading to excessive blood loss.
authors.affiliation: Department of Women's and Children's Health, Karolinska Institutet at Karolinska University Hospital Solna, S-17176 Stockholm, Sweden.
authors.forename: Emil
authors.initials: E
authors.lastname: Andersson
authors.affiliation: Departments of Medicine and Hematology, Karolinska Institutet and Karolinska University Hospital Huddinge, S-14186 Stockholm, Sweden.
authors.forename: Eva
authors.initials: E
authors.lastname: Zetterberg
authors.affiliation: Department of Coagulation, Skånes University Hospital, S-21428 Malmö, Sweden.
authors.forename: Inger
authors.initials: I
authors.lastname: Vedin
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, S-14186 Stockholm, Sweden.
authors.forename: Kjell
authors.initials: K
authors.lastname: Hultenby
authors.affiliation: Department of Coagulation, Skånes University Hospital, S-21428 Malmö, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Palmblad
authors.affiliation: Department of Women's and Children's Health, Karolinska Institutet at Karolinska University Hospital Solna, S-17176 Stockholm, Sweden.
authors.forename: Miriam
authors.initials: M
authors.lastname: Mints
mesh_terms: D000199:Actins / Q000096:biosynthesis; D000328:Adult; D004717:Endometrium / Q000098:blood supply* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008595:Menorrhagia / Q000378:metabolism* / Q000473:pathology; D055806:Microvessels / Q000378:metabolism* / Q000473:pathology; D020286:Pericytes / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A