title: Characterisation of non-autoinducing tropodithietic Acid (TDA) production from marine sponge Pseudovibrio species.
abstract: The search for new antimicrobial compounds has gained added momentum in recent years, paralleled by the exponential rise in resistance to most known classes of current antibiotics. While modifications of existing drugs have brought some limited clinical success, there remains a critical need for new classes of antimicrobial compound to which key clinical pathogens will be naive. This has provided the context and impetus to marine biodiscovery programmes that seek to isolate and characterize new activities from the aquatic ecosystem. One new antibiotic to emerge from these initiatives is the antibacterial compound tropodithietic acid (TDA). The aim of this study was to provide insight into the bioactivity of and the factors governing the production of TDA in marine Pseudovibrio isolates from a collection of marine sponges. The TDA produced by these Pseudovibrio isolates exhibited potent antimicrobial activity against a broad spectrum of clinical pathogens, while TDA tolerance was frequent in non-TDA producing marine isolates. Comparative genomics analysis suggested a high degree of conservation among the tda biosynthetic clusters while expression studies revealed coordinated regulation of TDA synthesis upon transition from log to stationary phase growth, which was not induced by TDA itself or by the presence of the C10-acyl homoserine lactone quorum sensing signal molecule.
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. catriona.harrington@gmail.com.
authors.forename: Catriona
authors.initials: C
authors.lastname: Harrington
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. J.Reen@ucc.ie.
authors.forename: F Jerry
authors.initials: FJ
authors.lastname: Reen
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. marlies.mooij@mumc.nl.
authors.forename: Marlies J
authors.initials: MJ
authors.lastname: Mooij
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. fiona.stewart204@gmail.com.
authors.forename: Fiona A
authors.initials: FA
authors.lastname: Stewart
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. jeanba-cha@hotmail.fr.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Chabot
authors.affiliation: Microbial Genomics and Bioinformatics Research Group, Max Planck Institute for Marine Microbiology, Bremen D-28359, Germany. afernand@mpi-bremen.de.
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Guerra
authors.affiliation: Microbial Genomics and Bioinformatics Research Group, Max Planck Institute for Marine Microbiology, Bremen D-28359, Germany. fog@mpi-bremen.de.
authors.forename: Frank O
authors.initials: FO
authors.lastname: Glöckner
authors.affiliation: Department of Systems Biology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. kfn@bio.dtu.dk.
authors.forename: Kristian F
authors.initials: KF
authors.lastname: Nielsen
authors.affiliation: Department of Systems Biology, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark. gram@bio.dtu.dk.
authors.forename: Lone
authors.initials: L
authors.lastname: Gram
authors.affiliation: Environmental Research Institute, University College Cork-National University of Ireland, Cork, Ireland. a.dobson@ucc.ie.
authors.forename: Alan D W
authors.initials: AD
authors.lastname: Dobson
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. c.adams@ucc.ie.
authors.forename: Claire
authors.initials: C
authors.lastname: Adams
authors.affiliation: BIOMERIT Research Centre, School of Microbiology, University College Cork-National University of Ireland, Cork, Ireland. f.ogara@ucc.ie.
authors.forename: Fergal
authors.initials: F
authors.lastname: O'Gara
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D011161:Porifera / Q000737:chemistry*; D014334:Tropolone / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; C527336:tropodithietic acid; D014334:Tropolone