title: Novel phage group infecting Lactobacillus delbrueckii subsp. lactis, as revealed by genomic and proteomic analysis of bacteriophage Ldl1.
abstract: Ldl1 is a virulent phage infecting the dairy starter Lactobacillus delbrueckii subsp. lactis LdlS. Electron microscopy analysis revealed that this phage exhibits a large head and a long tail and bears little resemblance to other characterized phages infecting Lactobacillus delbrueckii. In vitro propagation of this phage revealed a latent period of 30 to 40 min and a burst size of 59.9 +/- 1.9 phage particles. Comparative genomic and proteomic analyses showed remarkable similarity between the genome of Ldl1 and that of Lactobacillus plantarum phage ATCC 8014-B2. The genomic and proteomic characteristics of Ldl1 demonstrate that this phage does not belong to any of the four previously recognized L. delbrueckii phage groups, necessitating the creation of a new group, called group e, thus adding to the knowledge on the diversity of phages targeting strains of this industrially important lactic acid bacterial species.
authors.forename: Eoghan
authors.initials: E
authors.lastname: Casey
authors.forename: Jennifer
authors.initials: J
authors.lastname: Mahony
authors.forename: Horst
authors.initials: H
authors.lastname: Neve
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Noben
authors.forename: Fabio
authors.initials: F
authors.lastname: Dal Bello
authors.forename: Douwe
authors.initials: D
authors.lastname: van Sinderen
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D016679:Genome, Viral; D023281:Genomics; D048668:Lactobacillus delbrueckii / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D040901:Proteomics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins