title: Chemical structure of the arabinogalactan protein from gum ghatti and its interaction with bovine serum albumin.
abstract: Exudate gums, because of their beneficial properties, have been significant items of international trade in various industries for centuries. This manuscript sets out to gain insight into the fine structural details of an arabinogalactan protein (AGP) of gum ghatti (Anogeissus latifolia gum). The presence of a highly branched 554 kDa AGP having 1,6-linked Galp, 1,2-linked Manp, 1,3-linked Araf and 1,4-linked GlcpA main chain, substituted at O-4,6 of 1,2-linked Manp, and O-3/O-3,4 of 1,6-linked Galp residues by Araf, Arap and Galp units was revealed by chemical, chromatographic, ESMS, and NMR analyses. In particular, ESMS analysis of per acetylated oligomeric fragments derived from AGP by Smith degradation followed by acetylation was described as a commanding tool for providing critical structural information on a spectrum of glycerol tagged oligosaccharides. In addition, formation of an electrostatically driven complex between the isolated AGP and bovine serum albumin resulting in changes in the microenvironment around the tryptophan residues of BSA was established. A moderate radical scavenging activity comparable with those of standard antioxidants was observed from the AGP fraction (∼94% at 1 mg/mL) that could be valuable in foods or pharmaceutical products as alternatives to synthetic antioxidants.
authors.affiliation: Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan 713 104, West Bengal, India.
authors.forename: Kanika
authors.initials: K
authors.lastname: Ghosh
authors.affiliation: Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan 713 104, West Bengal, India.
authors.forename: Sayani
authors.initials: S
authors.lastname: Ray
authors.affiliation: Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan 713 104, West Bengal, India.
authors.forename: Debjani
authors.initials: D
authors.lastname: Ghosh
authors.affiliation: Natural Products Laboratory, Department of Chemistry, The University of Burdwan, Golapbag, Burdwan 713 104, West Bengal, India. Electronic address: bimalendu_ray@yahoo.co.uk.
authors.forename: Bimalendu
authors.initials: B
authors.lastname: Ray
mesh_terms: D009088:Mucoproteins / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D009088:Mucoproteins; D053149:Plant Gums; D010940:Plant Proteins; C006619:arabinogalactan proteins; D012710:Serum Albumin, Bovine; C015253:gum ghatti
keywords: AGP–BSA interaction; Anogeissus latifolia gum; Arabinogalactan protein; ESMS analysis; Smith degradation