title: [Application of near-infrared spectroscopy technology in extraction and concentration process of Reduning injection].
abstract: OBJECTIVE
  To establish a rapid quantitative analysis method for the content of chlorogenic acid and solid content in the extraction liquid concentration process during the production of Reduning injection by using the near-infrared (NIR) spectroscopy, in order to reflect the concentration state in a real-time manner and really realize the quality control of concentrating process of the extraction and concentration process.
  
  
  METHODS
  The samples during the Jinqing extraction liquid concentration process were collected. After the removal of abnormal samples, the spectra pretreatment and the wave band selection, the quantitative calibration model between NIR spectra and chlorogenic acid HPLC analytical value and solid content was established by using PLS algorithm, and unknown samples were predicted.
  
  
  RESULTS
  The correlation coefficients between the chlorogenic acid content and the solid content were respectively 0.992 1 and 0.994 0, and the correlation coefficients of the verification model were respectively 0.994 4 and 0.998 4, with the root mean square error of calibration (RMSEC) of 0.814 6 and 2.656 1 and the root mean square error of prediction (RMSEP) of 0.704 6 and 1.876 7 respectively, and the relative standard errors of predictions (RSEP) were 6.01% and 2.93% respectively.
  
  
  CONCLUSIONS
  The method is simple, rapid, nondestructive, accurate and reliable, thus could be adopted for the fast monitoring of the chlorogenic acid content and the solid content during the concentration process of Reduning injection extraction liquid.
authors.forename: Ya-Fei
authors.initials: YF
authors.lastname: Zhang
authors.forename: Xiang-Yun
authors.initials: XY
authors.lastname: Zuo
authors.forename: Yu-An
authors.initials: YA
authors.lastname: Bi
authors.forename: Jian-Xiong
authors.initials: JX
authors.lastname: Wu
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: L
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D011786:Quality Control; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal