title: [Effect of manual acupuncture stimulation of "Baihui" (GV 20) and "Dazhui" (GV 14) on contents of 5-HT, dopamine and ACh and expression of 5-HT mRNA, DA mRNA and AChE mRNA in the hippocampus in methamphetamine addiction rats].
abstract: OBJECTIVE
  To observe the effect of manual acupuncture stimulation on changes of hippocampal monoamine neurotransmitter levels and expression of 5-hydorxytryptamine (5-HT) mRNA, dopamine (DA) mRNA and acetylcholine esterase (AChE) mRNA in methamphetamine addiction rats, so as to explore its mechanism underlying improvement of drug addiction.
  
  
  METHODS
  SD rats were randomly divided into normal control, model and manual acupuncture groups (n=10 in each group). Drug addiction model was established by i.p. of methamphetamine (5 mg/kg), once a day for 15 days. Manual acupuncture stimu- lation was applied to "Baihui" (GV 20) and "Dazhui" (GV 14) once daily for 10 days. The contents of hippocampal 5-HT, DA, acetylcholine (ACh), AChE were measured by ELISA. The expressive Ilieels of hippocampal 5-HT mRNA, DA mRNA and AChE mRNA were determined by fluorescence quantitative RT-POR.
  
  
  RESULTS
  In comparison with the normal control group, the con- tents of 5-HT, DA, ACh and AChE and the expression levels of 5-HT mRNA, DA mRNA and AChE mRNA were significantly increased in the model group (P<0.01, P<0.05). After acupuncture intervention, the levels of the above-mentioned 7 indexes were uniformly and significantly down-regulated in the manual acupuncture group (P<0.01, P<0.05).
  
  
  CONCLUSIONS
  Manual acupuncture stimulation of GV 20 and GV 14 can adjust methamphetamine addiction-induced changes of some hippocampal monoa- mine neurotransmitters and expression levels of 5-HT, DA and AChE genes.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yu
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Xing-hui
authors.initials: XH
authors.lastname: Cai
authors.forename: Xiao-ge
authors.initials: XG
authors.lastname: Song
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Song
authors.forename: Yi-fang
authors.initials: YF
authors.lastname: Li
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics* / Q000378:metabolism; D015669:Acupuncture Points; D015670:Acupuncture Therapy; D000818:Animals; D004298:Dopamine / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D006801:Humans; D008297:Male; D008694:Methamphetamine / Q000009:adverse effects*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism*; D019966:Substance-Related Disorders / Q000235:genetics / Q000378:metabolism / Q000628:therapy*
chemical_list: D012333:RNA, Messenger; D012701:Serotonin; D008694:Methamphetamine; D000110:Acetylcholinesterase; D004298:Dopamine