title: Genotyping of Canine parvovirus in western Mexico.
abstract: Canine parvovirus (CPV) is one of the most common infectious agents related to high morbidity rates in dogs. In addition, the virus is associated with severe gastroenteritis, diarrhea, and vomiting, resulting in high death rates, especially in puppies and nonvaccinated dogs. To date, there are 3 variants of the virus (CPV-2a, CPV-2b, and CPV-2c) circulating worldwide. In Mexico, reports describing the viral variants circulating in dog populations are lacking. In response to this deficiency, a total of 41 fecal samples of suspected dogs were collected from October 2013 through April 2014 in the Veterinary Hospital of the University of Guadalajara in western Mexico. From these, 24 samples resulted positive by polymerase chain reaction, and the viral variant was determined by restriction fragment length polymorphism. Five positive diagnosed samples were selected for partial sequencing of the vp2 gene and codon analysis. The results demonstrated that the current dominant viral variant in Mexico is CPV-2c. The current study describes the genotyping of CPV strains, providing valuable evidence of the dominant frequency of this virus in a dog population from western Mexico.
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga) cpedroza46@gmail.com.
authors.forename: César
authors.initials: C
authors.lastname: Pedroza-Roldán
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga).
authors.forename: Varinia
authors.initials: V
authors.lastname: Páez-Magallan
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga).
authors.forename: Claudia
authors.initials: C
authors.lastname: Charles-Niño
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga).
authors.forename: Darwin
authors.initials: D
authors.lastname: Elizondo-Quiroga
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga).
authors.forename: Raúl Leonel
authors.initials: RL
authors.lastname: De Cervantes-Mireles
authors.affiliation: Department of Veterinary Medicine, University Center for Biological and Agricultural Sciences (CUCBA), University of Guadalajara, Zapopan, Jalisco, Mexico (Pedroza-Roldán, Páez-Magallan, De Cervantes-Mireles, López-Amezcua)Department of Microbiology and Pathology, University Center for Health Sciences (CUCS), University of Guadalajara, Guadalajara, Jalisco, Mexico (Charles-Niño)Medical and Pharmaceutical Biotechnology Unit, Center for Research and Applied Technology in Jalisco (CIATEJ)Guadalajara, Jalisco, Mexico (Elizondo-Quiroga).
authors.forename: Mario Alberto
authors.initials: MA
authors.lastname: López-Amezcua
mesh_terms: D000818:Animals; D003062:Codon; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D005260:Female; D005838:Genotype; D008297:Male; D008800:Mexico; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D003062:Codon; D014764:Viral Proteins
keywords: Canine parvovirus; Mexico; genotyping