title: Diversity and biotransformative potential of endophytic fungi associated with the medicinal plant Kadsura angustifolia.
abstract: This study investigated the diversity and host component-transforming activity of endophytic fungi in medicinal plant Kadsura angustifolia. A total of 426 isolates obtained were grouped into 42 taxa belonging to Fungi Imperfecti (65.96%), Ascomycota (27.00%), Zygomycota (1.64%), Basidiomycota (0.47%) and Mycelia Sterilia (4.93%). The abundance, richness, and species composition of endophytic assemblages were significantly dependent on the tissue and the sampling site. Many phytopathogenic species associated with healthy K. angustifolia were found prevalent. Among them, Verticillium dahliae was dominant with 16.43% abundance. From 134 morphospecies selected, 39 showed remarkable biocatalytic activity and were further identified as species belonging to the genera Colletotrichum, Eupenicillium, Fusarium, Hypoxylon, Penicillium, Phomopsis, Trametes, Trichoderma, Umbelopsis, Verticillium and Xylaria on the basis of the sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2). The results obtained in this work show that K. angustifolia is an interesting reservoir of pathogenic fungal species, and could be a community model for further ecological and evolutionary studies. Additionally, the converting potency screening of some endophytic fungi from this specific medicinal plant may provide an interesting niche on the search for novel biocatalysts.
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: An
authors.affiliation: Provincial Key Lab of Rural Energy Engineering, Yunnan Normal University, Kunming 650092, China.
authors.forename: Hongchuan
authors.initials: H
authors.lastname: Song
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Mao
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Shen
authors.affiliation: Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Science, Southwest University, Chongqing 400715, China. Electronic address: donjyaa@swu.edu.cn.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Dong
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D001487:Basidiomycota / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D044822:Biodiversity; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D005658:Fungi / Q000302:isolation & purification* / Q000378:metabolism*; D032135:Kadsura / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D018547:Plant Stems / Q000382:microbiology*; D010946:Plants, Medicinal / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013559:Symbiosis; D020172:Verticillium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Biotransformation; Diversity; Endophytic fungi; Kadsura angustifolia; Verticillium dahliae