title: Enzymatic synthesis of modified oligonucleotides by PEAR using Phusion and KOD DNA polymerases.
abstract: Antisense synthetic oligonucleotides have been developed as potential gene-targeted therapeutics. We previously reported polymerase-endonuclease amplification reaction (PEAR) for amplification of natural and 5'-O-(1-thiotriphosphate) (S)-modified oligonucleotides. Here, we extended the PEAR technique for enzymatic preparation of 2'-deoxy-2'-fluoro-(2'-F) and 2'-F/S double-modified oligonucleotides. The result showed that KOD and Phusion DNA polymerase could synthesize oligonucleotides with one or two modified nucleotides, and KOD DNA polymerase is more suitable than Phusion DNA polymerase for PEAR amplification of 2'-F and 2'-F/S double modified oligonucleotides. The composition of PEAR products were analyzed by electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS) detection and showed that the sequence of the PEAR products are maintained at an extremely high accuracy (>99.9%), and after digestion the area percent of full-length modified oligonucleotides reaches 89.24%. PEAR is suitable for synthesis of modified oligonucleotides efficiently and with high purity.
authors.affiliation: Department of Biotechnology, College of Marine Life Sciences, Ocean University of China , Qingdao, Shandong Province, China .
authors.forename: Xuxiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jianye
authors.initials: J
authors.lastname: Zhang
authors.forename: Yingjia
authors.initials: Y
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Wang
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry*; D055162:Biocatalysis; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D020319:Oligodeoxyribonucleotides, Antisense / Q000138:chemical synthesis*
chemical_list: D019843:Archaeal Proteins; D005456:Fluorescent Dyes; D020319:Oligodeoxyribonucleotides, Antisense; D004259:DNA-Directed DNA Polymerase