title: Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway.
abstract: One purified polysaccharide protein tyrosine phosphatase (PTP) was isolated from the roots of Polygala tenuifolia. The aim of the present study is to investigate the antitumor effect of PTP on human ovarian cancer OVCAR-3 cells and explore the molecular mechanism of the action involved. The results of MTT assay and apoptosis detection assay showed that PTP inhibited cellular proliferation of OVCAR-3 cells and induced apoptotic cellular death via arresting cell circle at the G0/G1 phase. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis identified that bcl-2 gradually decreased at both transcription and protein levels after PTP treatment for 48 h in OVCAR-3 cells, while those of bax, cytochrome c, caspase-3, and caspase-9 increased. In addition, the low expression of NF-κB in PTP-treated OVCAR-3 cells would trigger the extrinsic pathway of programmed cell death signaling in tumor cells. These results together suggest that PTP may induce apoptosis of OVCAR-3 cells through a mitochondrial pathway.
authors.affiliation: Department of Gynecology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin, 150040, China.
authors.forename: Fubin
authors.initials: F
authors.lastname: Zhang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Song
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Jingfang
authors.initials: J
authors.lastname: Wang
authors.forename: Leyuan
authors.initials: L
authors.lastname: Lin
authors.forename: Cong
authors.initials: C
authors.lastname: Li
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.forename: Yanju
authors.initials: Y
authors.lastname: Lv
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Jin
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Hu
authors.forename: Tao
authors.initials: T
authors.lastname: Xin
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000187:drug effects*; D016328:NF-kappa B / Q000096:biosynthesis; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D031801:Polygala / Q000737:chemistry; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D011134:Polysaccharides