title: A novel RNA in situ hybridization assay for the long noncoding RNA SChLAP1 predicts poor clinical outcome after radical prostatectomy in clinically localized prostate cancer.
abstract: Long noncoding RNAs (lncRNAs) are an emerging class of oncogenic molecules implicated in a diverse range of human malignancies. We recently identified SChLAP1 as a novel lncRNA that demonstrates outlier expression in a subset of prostate cancers, promotes tumor cell invasion and metastasis, and associates with lethal disease. Based on these findings, we sought to develop an RNA in situ hybridization (ISH) assay for SChLAP1 to 1) investigate the spectrum of SChLAP1 expression from benign prostatic tissue to metastatic castration-resistant prostate cancer and 2) to determine whether SChLAP1 expression by ISH is associated with outcome after radical prostatectomy in patients with clinically localized disease. The results from our current study demonstrate that SChLAP1 expression increases with prostate cancer progression, and high SChLAP1 expression by ISH is associated with poor outcome after radical prostatectomy in patients with clinically localized prostate cancer by both univariate (hazard ratio = 2.343, P = .005) and multivariate (hazard ratio = 1.99, P = .032) Cox regression analyses. This study highlights a potential clinical utility for SChLAP1 ISH as a novel tissue-based biomarker assay for outcome prognostication after radical prostatectomy.
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI; Comprehensive Cancer Center, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI. Electronic address: mrohit@med.umich.edu.
authors.forename: Rohit
authors.initials: R
authors.lastname: Mehra
authors.affiliation: Michigan Center for Translational Pathology, Ann Arbor, MI; Department of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Udager
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI.
authors.forename: John R
authors.initials: JR
authors.lastname: Prensner
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI.
authors.forename: Anirban
authors.initials: A
authors.lastname: Sahu
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI.
authors.forename: Matthew K
authors.initials: MK
authors.lastname: Iyer
authors.affiliation: Michigan Center for Translational Pathology, Ann Arbor, MI.
authors.forename: Javed
authors.initials: J
authors.lastname: Siddiqui
authors.affiliation: Michigan Center for Translational Pathology, Ann Arbor, MI.
authors.forename: Xuhong
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Urology, University of Michigan Health System, Ann Arbor, MI.
authors.forename: John
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Biostatistics, University of Michigan School of Public Health, Ann Arbor, MI.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Comprehensive Cancer Center, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI; Department of Radiation Oncology, University of Michigan Health System, Ann Arbor, MI.
authors.forename: Felix Y
authors.initials: FY
authors.lastname: Feng
authors.affiliation: Department of Pathology, University of Michigan Health System, Ann Arbor, MI; Comprehensive Cancer Center, University of Michigan Health System, Ann Arbor, MI; Michigan Center for Translational Pathology, Ann Arbor, MI; Department of Urology, University of Michigan Health System, Ann Arbor, MI; Howard Hughes Medical Institute, Ann Arbor, MI. Electronic address: arul@med.umich.edu.
authors.forename: Arul M
authors.initials: AM
authors.lastname: Chinnaiyan
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics*; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000235:genetics*; D012313:RNA / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012313:RNA