title: Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.
abstract: This study evaluated the messenger RNA (mRNA) expression and immunolocalization of all members of the platelet-derived growth factor (PDGF) family in caprine ovaries by quantitative PCR and immunohistochemistry, respectively. Detectable levels of PDGF-A mRNA were not observed in primordial follicles. Higher levels of PDGF-B mRNA were observed in primary follicles than in primordial follicles (P < 0.05). PDGF-D mRNA levels were higher in secondary follicles than in the other preantral follicle categories (P < 0.05). PDGF-B mRNA expression was higher than PDGF-C mRNA expression in primary follicles (P < 0.05). In antral follicles, PDGF-A mRNA expression was higher in cumulus-oocyte complexes (COCs) from small antral follicles than in those from large antral follicles and their respective granulosa/theca (GT) cells (P < 0.05). Furthermore, in COCs from small and large antral follicles, PDGF-A mRNA expression was higher than that of the other PDGF isoforms (P < 0.05). The mRNA levels of PDGF-B and PDGF-D and PDGFR-α and PDGFR-β were higher in GT cells from large antral follicles than in GT cells from small antral follicles and in their respective COCs (P < 0.05). In COCs and GT cells from small antral follicles, the mRNA levels of PDGFR-α were higher than those of PDGFR-β (P < 0.05). All proteins were observed in the cytoplasm of oocytes from all follicular categories. In granulosa cells, all PDGFs and PDGFR-β were detected from starting at the secondary stage, and in theca cells, all proteins, except PDGF-C, were detected starting at the antral stage. In conclusion, PDGF and its receptors are differentially expressed in the oocytes and ovarian cells according to the stage of follicular development, suggesting their role in the regulation of folliculogenesis in goats.
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: ivinabrito@yahoo.com.br.
authors.forename: I R
authors.initials: IR
authors.lastname: Brito
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A D
authors.initials: AD
authors.lastname: Sales
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: C H
authors.initials: CH
authors.lastname: Lobo
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: S V
authors.initials: SV
authors.lastname: Castro
authors.affiliation: Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceará, Sobral, CE, Brazil.
authors.forename: A W B
authors.initials: AW
authors.lastname: Silva
authors.affiliation: Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A A A
authors.initials: AA
authors.lastname: Moura
authors.affiliation: Biotechnology Nucleus of Sobral (NUBIS), Federal University of Ceará, Sobral, CE, Brazil.
authors.forename: J R V
authors.initials: JR
authors.lastname: Silva
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: A P R
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil.
authors.forename: J R
authors.initials: JR
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006041:Goats / Q000378:metabolism*; D006107:Granulosa Cells / Q000737:chemistry; D007150:Immunohistochemistry / Q000662:veterinary; D009865:Oocytes / Q000737:chemistry; D006080:Ovarian Follicle / Q000737:chemistry; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000032:analysis; D017479:Receptors, Platelet-Derived Growth Factor / Q000032:analysis / Q000235:genetics*; D013799:Theca Cells / Q000737:chemistry
chemical_list: D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D017479:Receptors, Platelet-Derived Growth Factor
keywords: Goat; Oocyte; Ovarian follicle; Protein; mRNA