title: Damage to the optic chiasm in myelin oligodendrocyte glycoprotein-experimental autoimmune encephalomyelitis mice.
abstract: Optic chiasm lesions in myelin oligodendrocyte glycoprotein (MOG)-experimental autoimmune encephalomyelitis (EAE) mice were characterized using magnetic resonance imaging (MRI) and validated using electron microscopy (EM). MR images were collected from 3 days after induction to remission, approximately 20 days after induction. Hematoxylin and eosin, solochrome cyanin-stained sections, and EM images were obtained from the optic chiasms of some mice approximately 4 days after disease onset when their scores were thought to be the highest. T2-weighted imaging and apparent diffusion coefficient map hyperintensities corresponded to abnormalities in the optic chiasms of EAE mice. Mixed inflammation was concentrated at the lateral surface. Degeneration of oligodendrocytes, myelin, and early axonal damage were also apparent. A marked increase in chiasm thickness was observed. T2-weighted and diffusion-weighted MRI can detect abnormalities in the optic chiasms of MOG-EAE mice. MRI is an important method in the study of this model toward understanding optic neuritis.
authors.affiliation: Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Sheryl L
authors.initials: SL
authors.lastname: Herrera
authors.affiliation: Biomedical Engineering Program, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: Palmer
authors.affiliation: Biopsychology Program, University of Winnipeg, Winnipeg, Manitoba, Canada.
authors.forename: Heather
authors.initials: H
authors.lastname: Whittaker
authors.affiliation: Physics, University of Winnipeg, Winnipeg, Manitoba, Canada.
authors.forename: Blair Cardigan
authors.initials: BC
authors.lastname: Smith
authors.affiliation: Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Annie
authors.initials: A
authors.lastname: Kim
authors.affiliation: Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada. ; General Surgery, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Angela E
authors.initials: AE
authors.lastname: Schellenberg
authors.affiliation: Imaging Program, Lawson Health Research Institute, London, Ontario, Canada. ; Medical Biophysics, Western University, London, Ontario, Canada.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Thiessen
authors.affiliation: Radiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Buist
authors.affiliation: Pathology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Marc R
authors.initials: MR
authors.lastname: Del Bigio
authors.affiliation: Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada. ; Biomedical Engineering Program, University of Manitoba, Winnipeg, Manitoba, Canada. ; Physics, University of Winnipeg, Winnipeg, Manitoba, Canada. ; Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Manitoba, Canada. ; Radiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Melanie
authors.initials: M
authors.lastname: Martin
keywords: experimental autoimmune encephalomyelitis; magnetic resonance imaging; mouse; optic chiasm; pertussis toxin