title: In-fiber photo-immobilization of a bioactive surface.
abstract: We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.
authors.affiliation: Precision Measurements Group, Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore 638075, SingaporebNanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Sin.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Lee
authors.affiliation: Precision Measurements Group, Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore 638075, SingaporebNanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Sin.
authors.forename: Derrick
authors.initials: D
authors.lastname: Yong
authors.affiliation: Precision Measurements Group, Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore 638075, Singapore.
authors.forename: Xia
authors.initials: X
authors.lastname: Yu
authors.affiliation: Precision Measurements Group, Singapore Institute of Manufacturing Technology, 71 Nanyang Drive, Singapore 638075, Singapore.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: Nanyang Technological University, Division of Bioengineering, School of Chemical and Biomedical Engineering, 70 Nanyang Drive, Singapore 637457, Singapore.
authors.forename: Chi Chiu
authors.initials: CC
authors.lastname: Chan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry*; D055767:Immobilized Proteins; D038761:Photobleaching; D055668:Photochemical Processes; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D005452:Fluoresceins; D055767:Immobilized Proteins; C118194:biotin-4-fluorescein; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin