title: Effect of vaccination with a multivalent modified-live viral vaccine on reproductive performance in synchronized beef heifers.
abstract: Prebreeding vaccination should provide fetal and abortive protection against bovine viral diarrhea virus (BVDV) and bovine herpesvirus 1 (BoHV-1) but not impede reproduction when administered to cattle before estrus synchronization and breeding. The objective was to assess reproductive performance when naive beef heifers were vaccinated with modified-live viral (MLV) vaccine 2 days after unsynchronized estrus, and then revaccinated with MLV vaccine at 10 or 31 days before synchronized natural breeding. Sixty beef heifers naive to BVDV and BoHV-1 were randomly assigned to one of four treatment groups. Groups A and B (n = 20 per group) were vaccinated with MLV vaccine containing BVDV and BoHV-1 at 2 days after initial detected estrus, and then revaccinated 30 days later, which corresponded to 10 days (group A) or 31 days (group B) before synchronized natural breeding. Groups C and D (n = 10 per group) served as controls and were vaccinated with an inactivated vaccine that did not contain BVDV or BoHV-1 at the same time points as groups A and B, respectively. Estrous behavior was assessed using radio frequency technology. Estrus synchronization was performed, with initiation occurring at revaccination (groups A and C) or 21 days after revaccination (groups B and D). After synchronization, heifers were submitted to a bull breeding pasture for 45 days. At the end of the breeding period, heifers were assessed for pregnancy using ultrasonography. Progesterone concentrations were evaluated at estrus and 10 days after unsynchronized and synchronized estrus, at initial pregnancy check, and at the end of the study. All pregnant heifers in groups A and B and five pregnant heifers in group C were euthanized between 44 and 62 days of gestation and ovarian and conceptus tissues were assayed for BVDV and BoHV-1. Vaccination with MLV vaccine did not result in significant negative reproductive impact based on the duration of interestrus intervals, proportion of heifers exhibiting estrus within 5 days after synchronization, serum progesterone concentrations, pregnancy rates, and pregnancies in the first 5 days of the breeding season. Bovine viral diarrhea virus and BoHV-1 were not detected in luteal tissue, ovarian tissue, or fetal tissues. Use of MLV vaccine did not impede reproduction, when revaccination was performed at 10 or 31 days before synchronized natural breeding.
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA. Electronic address: walzpau@auburn.edu.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Walz
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Misty A
authors.initials: MA
authors.lastname: Edmondson
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Kay P
authors.initials: KP
authors.lastname: Riddell
authors.affiliation: Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Timothy D
authors.initials: TD
authors.lastname: Braden
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Gard
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Jenna
authors.initials: J
authors.lastname: Bayne
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Kellye S
authors.initials: KS
authors.lastname: Joiner
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: Patricia K
authors.initials: PK
authors.lastname: Galik
authors.affiliation: Boehringer Ingelheim Vetmedica, Inc., St. Joseph, Missouri, USA.
authors.forename: Sjoert
authors.initials: S
authors.lastname: Zuidhof
authors.affiliation: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
authors.forename: M Daniel
authors.initials: MD
authors.lastname: Givens
mesh_terms: D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000517:prevention & control*; D002417:Cattle / Q000097:blood / Q000502:physiology*; D001908:Diarrhea Viruses, Bovine Viral; D015237:Dinoprost / Q000008:administration & dosage / Q000494:pharmacology; D004958:Estradiol / Q000097:blood; D004973:Estrus Synchronization; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology; D006566:Herpesviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D007242:Herpesvirus 1, Bovine; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D014765:Viral Vaccines; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D015237:Dinoprost
keywords: Bovine herpesvirus 1 (BoHV-1); Bovine viral diarrhea virus (BVDV); Conception; Estrous cycle; Progesterone; Vaccination