title: Physicochemical properties of complex rhamnogalacturonan I from gelatinous cell walls of flax fibers.
abstract: The physicochemical properties of flax fiber cell wall rhamnogalacturonan I (RG-I) and its fragments, obtained after galactanase treatment (fraction G1), were characterized. RG-I retains its hydrodynamic volume after its molecular weight decreases by approximately half, as revealed by SEC. Two techniques, DLS and NMR, with different principles of diffusion experiment were used to establish the reasons for this property of RG-I. Three possible types of particles were revealed by DLS depending on the concentration of the RG-I and G1 solutions (2-2.5, 15-20, and 150-200 nm). It was determined by BPP-LED experiments that the backbone of the RG-I was 1.3-1.9-fold more mobile than the side chains. The obtained data suggest a novel type of pectin spatial organization-the formation of RG-I associates with the backbone at the periphery and the interaction between the side chains to form a core zone.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia.
authors.forename: Polina V
authors.initials: PV
authors.lastname: Mikshina
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia.
authors.forename: Bulat Z
authors.initials: BZ
authors.lastname: Idiyatullin
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Petrova
authors.affiliation: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Pr., 47, 119991 Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia.
authors.forename: Yuriy F
authors.initials: YF
authors.lastname: Zuev
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Lobachevsky Str. 2/31, P.O. Box 30, 420111 Kazan, Russia. Electronic address: gorshkova@mail.knc.ru.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Gorshkova
mesh_terms: D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D019597:Flax / Q000166:cytology*; D005690:Galactose / Q000737:chemistry; D005780:Gelatin / Q000378:metabolism*; D057446:Hydrodynamics; D008969:Molecular Sequence Data; D010368:Pectins / Q000737:chemistry* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: C042491:rhamnogalacturonan I; D010368:Pectins; D005780:Gelatin; D001616:beta-Galactosidase; D005690:Galactose
keywords: Aggregation; Flax fibers; Plant polysaccharides; Rhamnogalacturonan I; Self-diffusion