title: [Functional properties of smooth muscle cells in ascending aortic aneurysm].
abstract: Thoracic aortic aneurism (TAA) develops as a result of complex series of events that dynamically alter the structure and composition of the aortic vascular extracellular matrix (ECM). The main elements that alter the composition of aortic wall are smooth muscle cells (SMC). The purpose of the present work was to study alteration of smooth muscle cell functions derived from the patients with TAA and from healthy donors. As it is supposed that TAA associated with bicuspid aortic valve (BAV) and with tricuspid aortic valve (TAV) differ in their pathogenesis, we compared the SMC and tissues samples from BAV-, TAV-patients and healthy donors. We compared TAA patients' derived tissues and SMC to healthy donors' ones in several parameters: SMC growth, migration and apoptotic dynamics; metalloproteinase MMP2 and MMP9 activity (zymography) and elastin, collagen and fibrillin content (Western blot) in both tissue samples and cultured SMC. Proliferation ability of both BAV and TAV SMC was decreased comparing to donors cells; migration ability in scratch tests was increased in TAV-derived SMC comparing to donor cells. BAV-cells migration ability was not changed comparing to donor-SMC. Elastin content was decreased in TAA SMC comparing to donor cells whereas the content of fibrillin and collagen was not altered. At the same time elastin and collagen protein level was significantly higher in tissue samples of TAA patients comparing to donor-derived samples. SMS proliferation and migration ability is differently affected in TAV and BAV-associated TAA that supports the idea of different nature of these two groups of TAA. Also our data show that SMC functional properties are altered in TAA patients and these alterations could play a significant role in the disease pathogenesis.
authors.forename: D A
authors.initials: DA
authors.lastname: Kostina
authors.forename: I V
authors.initials: IV
authors.lastname: Voronkina
authors.forename: L V
authors.initials: LV
authors.lastname: Smagina
authors.forename: N D
authors.initials: ND
authors.lastname: Gavriliuk
authors.forename: O M
authors.initials: OM
authors.lastname: Moiseeva
authors.forename: O B
authors.initials: OB
authors.lastname: Irtiuga
authors.forename: V E
authors.initials: VE
authors.lastname: Uspenskiĭ
authors.forename: A A
authors.initials: AA
authors.lastname: Kostareva
authors.forename: A B
authors.initials: AB
authors.lastname: Malashicheva
mesh_terms: D001011:Aorta / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D017545:Aortic Aneurysm, Thoracic / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D001021:Aortic Valve / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017209:Apoptosis; D000082882:Bicuspid Aortic Valve Disease; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D000071837:Fibrillins; D006349:Heart Valve Diseases / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D008840:Microfilament Proteins / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000378:metabolism / Q000473:pathology*; D018785:Tricuspid Atresia / Q000150:complications / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D015415:Biomarkers; D000071837:Fibrillins; D008840:Microfilament Proteins; D003094:Collagen; D004549:Elastin; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9