title: Lactoylglutathione lyase, a critical enzyme in methylglyoxal detoxification, contributes to survival of Salmonella in the nutrient rich environment.
abstract: Glyoxalase I which is synonymously known as lactoylglutathione lyase is a critical enzyme in methylglyoxal (MG) detoxification. We assessed the STM3117 encoded lactoylglutathione lyase (Lgl) of Salmonella Typhimurium, which is known to function as a virulence factor, due in part to its ability to detoxify methylglyoxal. We found that STM3117 encoded Lgl isomerises the hemithioacetal adduct of MG and glutathione (GSH) into S-lactoylglutathione. Lgl was observed to be an outer membrane bound protein with maximum expression at the exponential growth phase. The deletion mutant of S. Typhimurium (Δlgl) exhibited a notable growth inhibition coupled with oxidative DNA damage and membrane disruptions, in accordance with the growth arrest phenomenon associated with typical glyoxalase I deletion. However, growth in glucose minimal medium did not result in any inhibition. Endogenous expression of recombinant Lgl in serovar Typhi led to an increased resistance and growth in presence of external MG. Being a metalloprotein, Lgl was found to get activated maximally by Co(2+) ion followed by Ni(2+), while Zn(2+) did not activate the enzyme and this could be attributed to the geometry of the particular protein-metal complex attained in the catalytically active state. Our results offer an insight on the pivotal role of the virulence associated and horizontally acquired STM3117 gene in non-typhoidal serovars with direct correlation of its activity in lending survival advantage to Salmonella spp.
authors.affiliation: a Department of Microbiology and Cell Biology ; Indian Institute of Science ; Bangalore , India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Chakraborty
authors.forename: Mayuri
authors.initials: M
authors.lastname: Gogoi
authors.forename: Dipshikha
authors.initials: D
authors.lastname: Chakravortty
mesh_terms: D000595:Amino Acid Sequence; D004249:DNA Damage / Q000235:genetics; D007791:Lactoylglutathione Lyase / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics; D011765:Pyruvaldehyde / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D016415:Sequence Alignment; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D037521:Virulence Factors; D011765:Pyruvaldehyde; D007791:Lactoylglutathione Lyase