title: Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium.
abstract: The aim of the present study was to examine the effects of luteotropic and luteolytic factors on the mRNA and protein levels of progesterone receptor isoforms A (PGRA) and B (PGRB) in the bovine endometrium. Endometrial slices from Days 6-10 and 17-20 of the oestrous cycle were treated with LH (100ngmL-1), oestradiol (E2; 1×10-8M), prostaglandin (PG) E2(1×10-6M) and PGF2α(1×10-6M) and the nitric oxide donor NONOate (1×10-4M); these treatments lasted for 6h for mRNA expression analysis and 24h for protein expression analysis. On Days 6-10 of the oestrous cycle PGRAB (PGRAB; the entire PGRA mRNA sequence is common to the PGRB mRNA sequence) mRNA expression in endometrial slices was enhanced by E2 treatment (P<0.001), whereas PGRB mRNA expression was increased by LH (P<0.001), E2 (P<0.05) and NONOate (P<0.05) treatment. On Days 17-20, PGRAB mRNA expression increased after E2 (P<0.001) and PGE2(P<0.05) treatment; PGRB mRNA expression was increased by PGE2(P<0.05) and PGF2α(P<0.01) treatment, but decreased by LH (P<0.05). On Days 6-10 protein levels of PGRA were stimulated by E2 (P<0.01), whereas PGRB protein levels were increased by LH (P<0.05) and E2 (P<0.05). On Days 17-20 of the oestrous cycle, PGRA protein levels were enhanced by E2 (P<0.05) and PGF2α(P<0.05), whereas PGRB protein levels were stimulated by PGE2(P<0.05) and PGF2α(P<0.001). These data suggest that luteotropic and luteolytic factors affect PGRA and PGRB mRNA and protein levels, and this may regulate the effects of progesterone on endometrial cells.
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Rekawiecki
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
authors.forename: Magdalena Karolina
authors.initials: MK
authors.lastname: Kowalik
authors.affiliation: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Kotwica
mesh_terms: D000818:Animals; D002417:Cattle; D015237:Dinoprost / Q000502:physiology; D004717:Endometrium / Q000502:physiology*; D004958:Estradiol / Q000502:physiology; D005260:Female; D003341:Luteolysis; D020033:Protein Isoforms / Q000502:physiology; D012333:RNA, Messenger; D011980:Receptors, Progesterone / Q000502:physiology*
chemical_list: D020033:Protein Isoforms; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D004958:Estradiol; D015237:Dinoprost