title: Lobaplatin inhibits growth of gastric cancer cells by inducing apoptosis.
abstract: OBJECTIVE
  To assess the anti-cancer effect of lobaplatin on human gastric cancer cells, and to explore the underlying molecular mechanisms.
  
  
  METHODS
  The human gastric cancer cell lines MKN-28, AGS and MKN-45 were used. The cytotoxicity of lobaplatin was detected using an MTS cell proliferation assay. Flow cytometry was used to detect cell apoptosis using Annexin V-FITC Apoptosis Detection Kit. The expression of apoptosis-regulated genes was examined at the protein level using Western blot.
  
  
  RESULTS
  Lobaplatin inhibited the proliferation of human gastric cancer cells and induced apoptosis, which may be associated with the up-regulation of Bax expression, poly(ADP-ribose) polymerase (PARP) cleavage, p53 expression and the reduction of Bcl-2 expression.
  
  
  CONCLUSIONS
  The cytotoxicity of lobaplatin may be due to its ability of inducing apoptosis of gastric cancer cells, which would support the potential use of lobaplatin for the therapy of gastric cancer.
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Chu-Yang
authors.initials: CY
authors.lastname: Yin
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Lin
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ye
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Yang
authors.affiliation: Chu-Yang Yin, Department of Breast Surgery, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China.
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Xiao
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003503:Cyclobutanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D009944:Organoplatinum Compounds / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D003503:Cyclobutanes; D009944:Organoplatinum Compounds; C066228:lobaplatin
keywords: Apoptosis; Gastric cancer; Lobaplatin