title: Forced expression of Nanog or Esrrb preserves the ESC status in the absence of nucleostemin expression.
abstract: Nucleostemin (NS) is a nucleolar GTP-binding protein that is involved in a plethora of functions including ribosomal biogenesis and maintenance of telomere integrity. In addition to its expression in cancerous cells, the NS gene is expressed in stem cells including embryonic stem cells (ESCs). Previous knockdown and knockout studies have demonstrated that NS is important to preserve the self-renewality and high expression levels of pluripotency marker genes in ESCs. Here, we found that forced expression of Nanog or Esrrb, but not other pluripotency factors, resulted in the dispensability of NS expression in ESCs. However, the detrimental phenotypes of ESCs associated with ablation of NS expression were not mitigated by forced expression of Rad51 or a nucleolar localization-defective NS mutant that counteracts the damage associated with loss of NS expression in other NS-expressing cells such as neural stem/progenitor cells. Thus, our results indicate that NS participates in preservation of the viability and integrity of ESCs, which is distinct from that in other NS-expressing cells.
authors.affiliation: Division of Developmental Biology, Saitama Medical University, Yamane, Hidaka, Saitama, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Katano
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Ema
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nakachi
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Mizuno
authors.forename: Masataka
authors.initials: M
authors.lastname: Hirasaki
authors.forename: Ayumu
authors.initials: A
authors.lastname: Suzuki
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ueda
authors.forename: Masazumi
authors.initials: M
authors.lastname: Nishimoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuda
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis*; D053595:Embryonic Stem Cells / Q000378:metabolism*; D019204:GTP-Binding Proteins; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D000071317:Nanog Homeobox Protein; D009687:Nuclear Proteins / Q000096:biosynthesis*; D016601:RNA-Binding Proteins; D011960:Receptors, Estrogen / Q000096:biosynthesis*
chemical_list: D002352:Carrier Proteins; C490918:ESRRB protein, human; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D011960:Receptors, Estrogen; C470311:nucleostemin protein, mouse; D019204:GTP-Binding Proteins
keywords: Embryonic stem cells; Epiblast stem cells; Leukemia inhibitory factor; Pluripotency