title: Biocompatibility of ferroelectric lithium niobate and the influence of polarization charge on osteoblast proliferation and function.
abstract: In this work, the influence of substrate surface charge on in vitro osteoblast cell proliferation on ferroelectric lithium niobate (LN) crystal surfaces is investigated. LN has a spontaneous polarization along the z-axis and is thus characterized by positive and negative bound polarization charge at the +z and -z surfaces. Biocompatibility of LN was demonstrated via culturing and fluorescence imaging of MC3T3 osteoblast cells for up to 11 days. The cells showed enhanced proliferation rates and improved osteoblast function through mineral formation on the positively and negatively charged LN surfaces compared to electrostatically neutral x-cut LN and a glass cover slip control. These results highlight the potential of LN as a template for investigating the role of charge on cellular processes.
authors.affiliation: School of Physics, University College Dublin, Belfield, Dublin 4, Ireland.|Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: N Craig
authors.initials: NC
authors.lastname: Carville
authors.affiliation: School of Physics, University College Dublin, Belfield, Dublin 4, Ireland.|Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Liam
authors.initials: L
authors.lastname: Collins
authors.affiliation: Department of Applied Physics, KTH-Royal Institute of Technology, Roslagstullbacken 21, 106 91 Stockholm, Sweden.
authors.forename: Michele
authors.initials: M
authors.lastname: Manzo
authors.affiliation: Department of Applied Physics, KTH-Royal Institute of Technology, Roslagstullbacken 21, 106 91 Stockholm, Sweden.
authors.forename: Katia
authors.initials: K
authors.lastname: Gallo
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Bart I
authors.initials: BI
authors.lastname: Lukasz
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.|School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Katey K
authors.initials: KK
authors.lastname: McKayed
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.|School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Jeremy C
authors.initials: JC
authors.lastname: Simpson
authors.affiliation: School of Physics, University College Dublin, Belfield, Dublin 4, Ireland.|Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Rodriguez
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D009556:Niobium / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D010087:Oxides; D009556:Niobium; C091692:lithium niobate
keywords: MC3T3; biocompatible; ferroelectric; inorganic ions; lithium niobate; polarization; surface charge