title: miR-143 inhibits interleukin-13-induced inflammatory cytokine and mucus production in nasal epithelial cells from allergic rhinitis patients by targeting IL13Rα1.
abstract: Allergic rhinitis (AR) is a common chronic inflammatory condition of the nasal mucosal tissue. The interleukin-13 (IL-13) signaling pathway is of great importance in the pathogenesis of AR. However, how the signaling molecules in this pathway are regulated, particularly through microRNAs (miRNAs), remains unclear. In the present study, we investigated the regulatory role and mechanism of miRNA-143 (miR-143) in IL-13-induced inflammatory cytokine and mucus production in nasal epithelial cells (NECs) from AR patients. Our results showed that forced expression of miR-143 significantly decreased the mRNA and protein expression levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), eotaxin and mucin 5AC (MUC5AC) in IL-13-stimulated NECs. Moreover, we confirmed that miR-143 directly targeted and significantly suppressed IL-13 receptor α1 chain (IL13Rα1) gene expression. This study thus suggests that miR-143 regulation of IL-13-induced inflammatory cytokine and mucus production in NECs from AR patients probably partly depends on inhibition of IL13Rα1. Therefore, the IL13Rα1 signaling pathway may be a potential target for the prevention and treatment of AR by miR-143.
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Yaoshu
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China. Electronic address: ruxinzhang2014@163.com.
authors.forename: Ruxin
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhuang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China.
authors.forename: Zhicong
authors.initials: Z
authors.lastname: Hong
mesh_terms: D000328:Adult; D054413:Chemokine CCL11 / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D018793:Interleukin-13 / Q000494:pharmacology*; D053661:Interleukin-13 Receptor alpha1 Subunit / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009077:Mucins / Q000235:genetics / Q000378:metabolism; D009093:Mucus / Q000187:drug effects / Q000378:metabolism*; D009297:Nasal Mucosa / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D065631:Rhinitis, Allergic / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects; D055815:Young Adult
chemical_list: D054413:Chemokine CCL11; C508181:IL13RA1 protein, human; D018836:Inflammation Mediators; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D009077:Mucins; D012333:RNA, Messenger; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Allergic rhinitis; Epithelial cell; Inflammation; Interleukin-13; miR-143