title: Optimization of ultrasonic-assisted extraction of cordycepin from Cordyceps militaris using orthogonal experimental design.
abstract: This study reports on the optimization of the extraction conditions of cordycepin from Cordyceps militaris by using ultrasonication. For this purpose, the orthogonal experimental design was used to investigate the effects of factors on the ultrasonic-assisted extraction (UAE). Four factors: extraction time (min), ethanol concentration (%), extraction temperature (°C) and extraction frequency (kHz), were studied. The results showed that the highest cordycepin yield of 7.04 mg/g (86.98% ± 0.23%) was obtained with an extraction time of 60 min, ethanol concentration of 50%, extraction temperature of 65 °C and extraction frequency of 56 kHz. It was found that the cordycepin extraction yield increased with the effect of ultrasonication during the extraction process. Therefore, UAE can be used as an alternative to conventional immersion extraction with respect to the recovery of cordycepin from C. militaris, with the advantages of shorter extraction time and reduced solvent consumption.
authors.affiliation: Department of Hospitality Management, Mingdao University, 369 Wen-Hua Rd, Peetow, Chang-Hua 52345, Taiwan. hjwang@mdu.edu.tw.
authors.forename: Hsiu-Ju
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: Department of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, Taiwan. juichen0812@gmail.com.
authors.forename: Meng-Chun
authors.initials: MC
authors.lastname: Pan
authors.affiliation: Department of BioIndustry Technology, Da-Yeh University, 168 University Rd, Dacun, Chang-Hua 51591, Taiwan. kai0913077636@gmail.com.
authors.forename: Chao-Kai
authors.initials: CK
authors.lastname: Chang
authors.affiliation: Department of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, Taiwan. swchang@mail.dyu.edu.tw.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Chang
authors.affiliation: Department of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, Taiwan. welson@mail.dyu.edu.tw.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Hsieh
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification*; D032661:Cordyceps / Q000737:chemistry*; D003839:Deoxyadenosines / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D012107:Research Design; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D013010:Sonication; D013016:Sound
chemical_list: D000970:Antineoplastic Agents; D003839:Deoxyadenosines; D012997:Solvents; D000431:Ethanol; C058120:cordycepin