title: Production of cell surface and secreted glycoproteins in mammalian cells.
abstract: Mammalian protein expression systems are becoming increasingly popular for the production of eukaryotic secreted and cell surface proteins. Here we describe methods to produce recombinant proteins in adherent or suspension human embryonic kidney cell cultures, using transient transfection or stable cell lines. The protocols are easy to scale up and cost-efficient, making them suitable for protein crystallization projects and other applications that require high protein yields.
authors.affiliation: The Division of Structural Biology, The Henry Wellcome Building for Genomic Medicine, Roosevelt Drive, Oxford, OX3 7BN, UK.
authors.forename: Elena
authors.initials: E
authors.lastname: Seiradake
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Weixian
authors.initials: W
authors.lastname: Lu
authors.forename: A Radu
authors.initials: AR
authors.lastname: Aricescu
authors.forename: E Yvonne
authors.initials: EY
authors.lastname: Jones
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D006023:Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014162:Transfection
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins