title: Human Adenomyosis Endometrium Stromal Cells Secreting More Nerve Growth Factor: Impact and Effect.
abstract: Abnormal expression of nerve growth factor (NGF) was found in adenomyosis (AM). We collected AM foci from patients and eutopic endometrium from non-AM controls. Endometrium stromal cells (ESCs) were cultured. Different levels of 17β-estradiol, tumor necrosis factor (TNF), CoCl2, and H2O2 were added to the culture system separately, then the expression level of NGF in ESCs was detected. After adding different levels of NGF, the proliferation and apoptosis of ESCs and aromatase expression were detected. We found that 17β-estradiol promoted NGF production in AM ESCs but not in control ESCs; TNF promoted NGF production in both AM and control ESCs; and CoCl2 inhibited NGF production in control ESCs, but had no effect in AM ESCs. Nerve growth factor promoted the proliferation and synthesis of aromatase in AM ESCs. In conclusion, locally increased estrogen levels and inflammation may cause increased NGF production in the uterus of patients with AM. Nerve growth factor stimulated the proliferation and increased aromatase expression of ESCs from AM foci, suggesting NGF might contribute to the pathology and etiology of AM.
authors.affiliation: Department of Gynaecology, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai, China Reproductive Medicine Center, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Gynaecology, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai, China.
authors.forename: Shien
authors.initials: S
authors.lastname: Zou
authors.affiliation: Department of Gynaecology, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Xia
authors.affiliation: Department of Gynaecology, Obstetrics and Gynaecology Hospital, Fudan University, Shanghai, China zhangshaofen@163.com.
authors.forename: Shaofen
authors.initials: S
authors.lastname: Zhang
mesh_terms: D062788:Adenomyosis / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D001141:Aromatase / Q000096:biosynthesis; D016022:Case-Control Studies; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008875:Middle Aged; D020932:Nerve Growth Factor / Q000378:metabolism*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: C502240:NGF protein, human; D014409:Tumor Necrosis Factor-alpha; D003035:Cobalt; D004958:Estradiol; D020932:Nerve Growth Factor; D006861:Hydrogen Peroxide; D001141:Aromatase; C561410:CYP19A1 protein, human; C018021:cobaltous chloride
keywords: Adenomyosis; aromatase; estrogen; nerve growth factor; proliferation