title: Residence times of receptors in dendritic spines analyzed by stochastic simulations in empirical domains.
abstract: Analysis of high-density superresolution imaging of receptors reveals the organization of dendrites at nanoscale resolution. We present here an apparently novel method that uses local statistics extracted from short-range trajectories for the simulations of long-range trajectories in empirical live cell images. Based on these empirical simulations, we compute the residence time of a receptor in dendritic spines that accounts for receptors' local interactions and geometrical membrane organization. We report here that depending on the type of the spine, the residence time varies from 1 to 5 min. Moreover, we show that there exists transient organized structures, previously described as potential wells that can regulate the trafficking of receptors to dendritic spine: the simulation results suggest that receptor trafficking is regulated by transient structures.
authors.affiliation: Group of Computational Biology and Applied Mathematics, Institut de Biologie de l'Ecole Normale Supérieure, Institut National de la Santé et de la Recherche Médicale 1024, Paris, France.
authors.forename: Nathanael
authors.initials: N
authors.lastname: Hoze
authors.affiliation: Group of Computational Biology and Applied Mathematics, Institut de Biologie de l'Ecole Normale Supérieure, Institut National de la Santé et de la Recherche Médicale 1024, Paris, France. Electronic address: david.holcman@ens.fr.
authors.forename: David
authors.initials: D
authors.lastname: Holcman
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D049229:Dendritic Spines / Q000378:metabolism*; D004058:Diffusion; D008959:Models, Neurological; D021381:Protein Transport; D051381:Rats; D018091:Receptors, AMPA / Q000378:metabolism*; D013995:Time
chemical_list: D018091:Receptors, AMPA