title: Oral exposure to the anti-pyridoxine compound 1-amino D-proline further perturbs homocysteine metabolism through the transsulfuration pathway in moderately vitamin B₆ deficient rats.
abstract: Pyridoxal 5'-phosphate (PLP; a B₆ vitamer) serves as an important cofactor in a myriad of metabolic reactions, including the transsulfuration (TS) pathway, which converts homocysteine (Hcy) to cysteine. While overt vitamin B₆ deficiency is rare, moderate deficiency is common and may be exacerbated by anti-pyridoxine factors in the food supply. To this end, we developed a model of moderate B₆ deficiency and a study was conducted to examine the in vivo effect of 1-amino D-proline (1ADP), an anti-pyridoxine factor found in flaxseed, on indices of Hcy metabolism through the TS pathway in moderately B₆ deficient rats. Male weaning rats received a semi-purified diet containing either 7 mg/kg (control; CD) or 0.7 mg/kg (moderately deficient; MD) diet of pyridoxine·hydrochloride (PN∙HCl), each with 1 of 4 levels of 1ADP, viz. 0, 0.1, 1 and 10 mg/kg diet for 5 weeks. Perturbations in vitamin B₆ biomarkers were more pronounced in the MD group. Plasma PLP was significantly reduced, while plasma Hcy (8-fold) and cystathionine (11-fold) were increased in rats consuming the highest amount of 1ADP in the MD group. The activities of hepatic cystathionine β-synthase and cystathionine γ-lyase enzymes were significantly reduced in rats consuming the highest 1ADP compared to the lowest, for both levels of PN∙HCl. Dilation of hepatic central veins and sinusoids, mild steatosis and increased liver triglycerides were present in MD rats consuming the highest 1ADP level. The current data provide evidence that the consumption of an anti-pyridoxine factor linked to flaxseed may pose a risk for subjects who are moderate/severe vitamin B₆ deficient.
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, Winnipeg R3T2N2, Canada.
authors.forename: Shyamchand
authors.initials: S
authors.lastname: Mayengbam
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, Winnipeg R3T2N2, Canada.
authors.forename: Sara
authors.initials: S
authors.lastname: Raposo
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, Winnipeg R3T2N2, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Aliani
authors.affiliation: Department of Human Nutritional Sciences, University of Manitoba, Winnipeg R3T2N2, Canada; Department of Animal Science, University of Manitoba, Winnipeg R3T2N2, Canada. Electronic address: james.house@umanitoba.ca.
authors.forename: James D
authors.initials: JD
authors.lastname: House
mesh_terms: D000818:Animals; D058070:Asymptomatic Diseases; D015415:Biomarkers / Q000097:blood; D003540:Cystathionine / Q000819:agonists / Q000097:blood; D003542:Cystathionine gamma-Lyase / Q000037:antagonists & inhibitors / Q000378:metabolism; D004032:Diet / Q000009:adverse effects*; D004195:Disease Models, Animal; D018450:Disease Progression; D019597:Flax / Q000009:adverse effects / Q000737:chemistry; D006710:Homocysteine / Q000097:blood / Q000378:metabolism*; D020138:Hyperhomocysteinemia / Q000209:etiology*; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000209:etiology; D011392:Proline / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D011732:Pyridoxal Phosphate / Q000037:antagonists & inhibitors / Q000097:blood / Q000172:deficiency; D011736:Pyridoxine / Q000037:antagonists & inhibitors* / Q000172:deficiency; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012639:Seeds / Q000009:adverse effects / Q000737:chemistry; D025101:Vitamin B 6 / Q000097:blood; D026681:Vitamin B 6 Deficiency / Q000097:blood / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*
chemical_list: D015415:Biomarkers; D006710:Homocysteine; D003540:Cystathionine; D011732:Pyridoxal Phosphate; C012179:1-aminoproline; D025101:Vitamin B 6; D011392:Proline; D003542:Cystathionine gamma-Lyase; D011736:Pyridoxine
keywords: 1-Amino d-proline; Anti-pyridoxine factor; Homocysteine metabolism; Linatine; Moderate vitamin B(6) deficiency; Nutritional co-insults; Pyridoxal 5′-phosphate