title: Identification and functional characterization of Siberian wild rye (Elymus sibiricus L.) small heat shock protein 16.9 gene (EsHsp16.9) conferring diverse stress tolerance in prokaryotic cells.
abstract: Small heat shock proteins (Hsps) protect against stress-inducible denaturation of substrates. Our objectives were to clone and examine the mRNA expression of the Hsp16.9 gene from Siberian wild rye grown under diverse stress treatments. We characterized EsHsp16.9 from Elymus sibiricus L. EsHsp16.9 has a 456-bp open reading frame that encodes a 151-amino acid protein with a conserved α-crystallin domain. Northern blot analysis showed that EsHsp16.9 transcripts were enhanced by heat, drought, arsenate, methyl viologen, and H2O2 treatment. In addition, recombinant EsHsp16.9 protein acts as a molecular chaperone to prevent the denaturation of malate dehydrogenase. Growth of cells overexpressing EsHsp16.9 was up to 200% more rapid in the presence of NaCl, arsenate, and polyethylene glycol than that of cells harboring an empty vector. These data suggest that EsHsp16.9 acts as a molecular chaperone that enhances stress tolerance in living organisms.
authors.affiliation: Grassland & Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 330-801, Republic of Korea, sanghoon@korea.kr.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Dong-Gi
authors.initials: DG
authors.lastname: Lee
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Son
authors.forename: Su Jung
authors.initials: SJ
authors.lastname: Park
authors.forename: Ki-Yong
authors.initials: KY
authors.lastname: Kim
authors.forename: Hyung Soo
authors.initials: HS
authors.lastname: Park
authors.forename: Joon-Yung
authors.initials: JY
authors.lastname: Cha
mesh_terms: D001149:Arsenates / Q000633:toxicity; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D003681:Dehydration; D031749:Elymus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D020869:Gene Expression Profiling; D050888:Heat-Shock Proteins, Small / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009997:Osmotic Pressure; D016877:Oxidants / Q000633:toxicity; D018384:Oxidative Stress; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013312:Stress, Physiological
chemical_list: D001149:Arsenates; D018744:DNA, Plant; D050888:Heat-Shock Proteins, Small; D016877:Oxidants; D011994:Recombinant Proteins; D012965:Sodium Chloride; C025657:arsenic acid