title: A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA.
abstract: A DNA nano-tweezer (DNA-NT) structure-based target mRNA detection probe, which uses fluorescence resonance energy transfer (FRET) as a detection signal and works as a single molecule, has been developed. This FRET-paired fluorescent dye-modified DNA-NT, self-assembled from three single-stranded DNAs, alters its structure from open to closed states and produces a FRET signal in response to in vitro transcripts of Hes-1 mRNA. Our results showed that the FRET-based DNA-NT detected both GLUT1 mRNA as a pre-fixed target mRNA model and Hes-1 mRNA as a model expressed inside a living cell. These results confirm the feasibility of using the FRET-based DNA-NT for imaging analysis of target mRNA.
authors.affiliation: Institute for Sustainable Sciences and Development, Hiroshima University, Higashihiroshima, Hiroshima 739-8511, Japan. hisafuna@hiroshima-u.ac.jp.
authors.forename: Hisakage
authors.initials: H
authors.lastname: Funabashi
authors.forename: Hajime
authors.initials: H
authors.lastname: Shigeto
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakatsuka
authors.forename: Akio
authors.initials: A
authors.lastname: Kuroda
mesh_terms: D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D002460:Cell Line; D004277:DNA, Single-Stranded / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D051272:Glucose Transporter Type 1 / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D000072056:Transcription Factor HES-1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D051272:Glucose Transporter Type 1; D018398:Homeodomain Proteins; D012333:RNA, Messenger; D000072056:Transcription Factor HES-1; C078493:HES1 protein, human