title: Barcoded microchips for biomolecular assays.
abstract: Multiplexed assay of analytes is of great importance for clinical diagnostics and other analytical applications. Barcode-based bioassays with the ability to encode and decode may realize this goal in a straightforward and consistent manner. We present here a microfluidic barcoded chip containing several sets of microchannels with different widths, imitating the commonly used barcode. A single barcoded microchip can carry out tens of individual protein/nucleic acid assays (encode) and immediately yield all assay results by a portable barcode reader or a smartphone (decode). The applicability of a barcoded microchip is demonstrated by human immunodeficiency virus (HIV) immunoassays for simultaneous detection of three targets (anti-gp41 antibody, anti-gp120 antibody, and anti-gp36 antibody) from six human serum samples. We can also determine seven pathogen-specific oligonucleotides by a single chip containing both positive and negative controls.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Zou
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jianzhong Jeff
authors.initials: JJ
authors.lastname: Xi
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D058893:DNA Barcoding, Taxonomic; D004279:DNA, Viral / Q000235:genetics; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015700:HIV Envelope Protein gp41 / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D046228:Microarray Analysis; D044085:Microfluidics / Q000379:methods*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology
chemical_list: D004279:DNA, Viral; D015699:HIV Envelope Protein gp120; D015700:HIV Envelope Protein gp41; D054299:env Gene Products, Human Immunodeficiency Virus; C059763:env protein gp36, Human immunodeficiency virus; C514787:gp41 protein, Human immunodeficiency virus 1