title: Accurate quantification of episomal HIV-1 two-long terminal repeat circles by use of optimized DNA isolation and droplet digital PCR.
abstract: Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasmid for normalization enables more accurate 2-LTR quantification than genomic DNA isolation.
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Eva
authors.initials: E
authors.lastname: Malatinkova
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Maja
authors.initials: M
authors.lastname: Kiselinova
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Pawel
authors.initials: P
authors.lastname: Bonczkowski
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Trypsteen
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Messiaen
authors.affiliation: Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Jolien
authors.initials: J
authors.lastname: Vermeire
authors.affiliation: Department of Clinical Chemistry, Microbiology and Immunology, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Bruno
authors.initials: B
authors.lastname: Verhasselt
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Karen
authors.initials: K
authors.lastname: Vervisch
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium Linos.Vandekerckhove@ugent.be.
authors.forename: Linos
authors.initials: L
authors.lastname: Vandekerckhove
authors.affiliation: HIV Translational Research Unit, Department of Internal Medicine, Faculty of Medicine and Health Sciences, Ghent University and Ghent University Hospital, Ghent, Belgium.
authors.forename: Ward
authors.initials: W
authors.lastname: De Spiegelaere
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D010957:Plasmids / Q000032:analysis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020079:Terminal Repeat Sequences
chemical_list: D004279:DNA, Viral