title: Multistage regulator based on tandem promoters and CRISPR/Cas.
abstract: Accurately controlling expression of target genes between several designed levels is essential for low-noise gene network and dynamic range of gene expression. However, such manipulations have been hard to achieve due to technical limitations. Based on tandem promoters and CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) system, we constructed a multistage regulator that could stably regulate the expression of the reporter gene on three levels, with more than 2-fold difference between each of them. Our findings provide novel insights into constructing a more powerful gene regulation system.
authors.affiliation: College of Life Sciences, Wuhan University , Wuhan, Hubei 430072, China.
authors.forename: Hangxing
authors.initials: H
authors.lastname: Jia
authors.forename: Tong
authors.initials: T
authors.lastname: Liang
authors.forename: Zhaoning
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhaoren
authors.initials: Z
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Liu
authors.forename: Linyi
authors.initials: L
authors.lastname: Tang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000235:genetics*; D005818:Genetic Engineering; D011401:Promoter Regions, Genetic / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*