title: Histopathological biomarkers in juvenile silver catfish (Rhamdia quelen) exposed to a sublethal lead concentration.
abstract: The aim of this study was to determine the 96-h lethal concentration (96-h LC50) of lead (Pb) in silver catfish, Rhamdia quelen, and to determine histopathological biomarkers in fish exposed for 96-h to a sublethal concentration at 25% of the LC50. The 96-h LC50 was 108 mgl(-1). In gills, the length and thickness of lamella and thickness of the filament epithelium were significantly higher in fish exposed to Pb for 48-h than in control fish whereas the interlamellar distance decreased. In the liver, the area occupied by lipid droplets and size of hepatocytes showed significantly higher values after 24-h of exposure. The percentage of abnormal renal tubules was higher in fish exposed to Pb, exhibiting a time-dependent increase. These variations in histopathological biomarkers permit the definition of the overall response of R. quelen to Pb and the potential usefulness in the monitoring of Pb contamination.
authors.affiliation: Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro de Investigaciones en Piscicultura Experimental (CIPEX), Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro Científico Tecnológico Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas - CONICET, Ocampo y Esmeralda, Rosario, Argentina. Electronic address: lautaro.munoz@unr.edu.ar.
authors.forename: Lautaro
authors.initials: L
authors.lastname: Muñoz
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000-Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Weber
authors.affiliation: Departamento de Química, Universidade Federal de Santa Maria, Avenida Roraima, 1000-Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Valderi
authors.initials: V
authors.lastname: Dressler
authors.affiliation: Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Avenida Roraima, 1000-Camobi, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Bernardo
authors.initials: B
authors.lastname: Baldisserotto
authors.affiliation: Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro de Investigaciones en Piscicultura Experimental (CIPEX), Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Bv. Ovidio Lagos y Ruta 33, 2170 Casilda, Argentina; Centro Científico Tecnológico Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas - CONICET, Ocampo y Esmeralda, Rosario, Argentina.
authors.forename: Fabricio Andrés
authors.initials: FA
authors.lastname: Vigliano
mesh_terms: D000818:Animals; D015415:Biomarkers; D002397:Catfishes; D004396:Coloring Agents; D003599:Cytoskeleton; D004848:Epithelium; D005880:Gills / Q000187:drug effects; D022781:Hepatocytes; D006863:Hydrogen-Ion Concentration; D007684:Kidney Tubules / Q000187:drug effects; D007854:Lead / Q000633:toxicity*; D007928:Lethal Dose 50; D066292:Lipid Droplets; D008099:Liver / Q000187:drug effects; D017747:Seafood; D012834:Silver; D013154:Spleen / Q000187:drug effects
chemical_list: D015415:Biomarkers; D004396:Coloring Agents; D007854:Lead; D012834:Silver
keywords: Acute toxicity; Histopathology; Lead; Rhamdia quelen