title: Production and characterisation of hyaluronidase and elastase inhibitory protein hydrolysates from Venus clam.
abstract: The hydrolysates of fresh and boiled Venus clams with five different proteases for the production of low-molecular protein hydrolysates were optimised by response surface methodology. Alcalase hydrolysates exhibited the strongest hyaluronidase inhibitory activity. The optimum hydrolysis conditions of fresh and boiled clams were< enzyme-to-substrate ratio (E/S), 2.15%; time, 150 min; water-to-substrate ratio (W/S), 83.84 mL g(-1) for fresh clam, and E/S, 2.02%; time, 4.11 h; W/S, 69.74 mL g(-1) for boiled clam. The fresh and boiled clam protein hydrolysates were fractionated by S-200 HR size-exclusion chromatography, which resulted in one (FH1) and two (BH1 and BH2) fractions, respectively. BH1 exhibited the highest hyaluronidase and elastase inhibitory activities with specific activities of 141.15 and 81.36% mL mg(-1), respectively. Therefore, the boiled Venus clam hydrolysate might be developed as a cosmeceutical agent because of its strong hyaluronidase and elastase inhibitory activities.
authors.affiliation: a Department of Marine Food Science and Technology , Gangneung-Wonju National University , Gangneung , Gangwon-do 210-702 , Korea.
authors.forename: Chanutchamon
authors.initials: C
authors.lastname: Sutthiwanjampa
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Kim
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors*; D011492:Protein Hydrolysates / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D011492:Protein Hydrolysates; D006821:Hyaluronoglucosaminidase; D010196:Pancreatic Elastase
keywords: Venus clam; elastase; hyaluronidase; protein hydrolysates; response surface methodology