title: Structure characterization of a novel polysaccharide from Dictyophora indusiata and its macrophage immunomodulatory activities.
abstract: A novel polysaccharide, here named DP1, was isolated from the fruiting body of Dictyophora indusiata using a water extraction method. Structure characterization revealed that DP1 had an average molecular weight of 1132 kDa and consisted of glucose (56.2%), galactose (14.1%), and mannose (29.7%). The main linkage type of DP1 were proven to be (1 → 3)-linked α-l-Man, (1 → 2,6)-linked α-d-Glc, (1 → 6)-linked β-d-Glc, (1 → 6)-linked β-d-Gal, and (1 → 6)-linked β-d-Man by periodate oxidation-Smith degradation and nuclear magnetic resonance analysis. The immunostimulating assay indicated that DP1 could significantly promote macrophage NO, TNF-α, and IL-6 secretion in murine RAW 264.7 cells involving complement receptor 3 (CR3). The immune activities of DP1 were quite stable under thermal processing (100, 121, and 145 °C). Besides, DP1 retained stability after acidic/alkline treatment (pH 4.0-10.0), which enabled it to be an ideal complementary medicine or functional food for therapeutics of hypoimmunity and immunodeficiency diseases.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou, Guangdong 510640, People's Republic of China.
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Liao
authors.forename: Zhen
authors.initials: Z
authors.lastname: Luo
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Ning
authors.forename: Jiguo
authors.initials: J
authors.lastname: Yang
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
mesh_terms: D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002460:Cell Line; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015850:Interleukin-6 / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D007155:Immunologic Factors; D015850:Interleukin-6; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha
keywords: Dictyophora indusiata; complement receptor 3; immunostimulating; polysaccharide; structure characterization