title: Clearance approach in hyperinsulinemic-euglycemic clamp evaluation in lean and obese subjects.
abstract: OBJECTIVE
  The main aim of this study was to propose a method to express whole body insulin sensitivity as estimated by a hyperinsulinemic-euglycemic clamp (HEC) as a dimensionless parameter.
  
  
  METHODS
  Two groups of subjects were examined: The first group was comprised of seven healthy lean volunteers with BMI <25 kg/m(2) and a second group comprised of four obese subjects with BMI ≥30 kg/m(2). The dependence between the M/I index expressing the whole body insulin sensitivity, and the dimensionless whole human body effect E as a ratio of the clearance of glucose and the clearance of insulin after their exogenous administration during the last 40 min of the HEC test, was expressed by regression analysis. Unlike an expression of insulin sensitivity/resistance as a function of M taking into account the space corrections or the M/I index, our whole human body effect represents the insulin sensitivity/resistance as a dimensionless number.
  
  
  RESULTS
  A linear dependence between the M/I index and the dimensionless effect E with zero intercept and slope at 2.2623 ± 0.157, r = 0.914, and between the M/I index and the effect E recalculated per kg of human body weight with zero intercept and slope at 0.03164 ± 0.00127, r = 0.978, were observed.
  
  
  CONCLUSIONS
  The high correlation between the M/I index and new effect E in lean and obese volunteers confirms our proposal that the HEC test could be evaluated by a dimensionless parameter which eliminates potential unit mismatches in the expression of clamp results.
authors.affiliation: Faculty of Mechanical Engineering, Institute of Automation, Measurement and Applied Informatics, Slovak University of Technology , Bratislava , Slovakia .
authors.forename: Ladislav
authors.initials: L
authors.lastname: Dedik
authors.forename: Jana
authors.initials: J
authors.lastname: Chrenova
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Rausova
authors.forename: Geltrude
authors.initials: G
authors.lastname: Mingrone
authors.forename: Adela
authors.initials: A
authors.lastname: Penesova
mesh_terms: D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D015992:Body Mass Index; D005260:Female; D015309:Glucose Clamp Technique / Q000379:methods*; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D009765:Obesity / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D007328:Insulin
keywords: Clearance; M/I; glucose; hyperinsulinemic–euglycemic clamp; insulin