title: Genetic variability in CYP2E1 and catalase gene among currently and formerly alcohol-dependent male subjects.
abstract: OBJECTIVE
  The present study explored whether specific single-nucleotide polymorphisms in alcohol metabolic pathway are associated with alcohol dependence or alcohol-related psychopathological symptoms.
  
  
  METHODS
  Three groups of male unrelated subjects were included: 101 currently alcohol-dependent patients, 100 formerly alcohol-dependent subjects and 97 healthy controls. The following questionnaires were implemented: AUDIT, Zung Depression and Anxiety scale, Brief Social Phobia Scale, Yale-Brown Obsessive Compulsive Scale, Obsessive Compulsive Drinking Scale and Buss-Durkee Hostility Inventory. All the subjects were genotyped for CYP2E1 c.-1053C>T and CAT c.-262C>T.
  
  
  RESULTS
  Statistically significant differences in the distribution of genotypes and alleles for CAT c.-262C>T polymorphism were observed among the three investigated groups. We observed a higher frequency of CAT -262T allele in alcohol-dependent subjects (OR = 1.74, 95% CI = 1.164-2.610). Among currently dependent patients CAT -262T allele carriers had higher AUDIT scores (P = 0.023), while CYP2E1-1053T allele carriers had significantly higher YBOCS-obsession subscale scores (P = 0.005) and Zung Anxiety Scale scores (P = 0.011).
  
  
  CONCLUSIONS
  Our findings suggest that the CAT c.-262C>T genetic polymorphism influences the susceptibility to alcohol dependence and severity of alcohol dependence, while CYP2E1 c.-1053C>T polymorphism influences the expression of obsessive-compulsive and anxiety symptoms.
authors.affiliation: Department of Psychiatry, University Clinical Center Maribor, Maribor, Slovenia.
authors.forename: Anja
authors.initials: A
authors.lastname: Plemenitas
authors.affiliation: Pharmacogenetics Laboratory, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Matej
authors.initials: M
authors.lastname: Kastelic
authors.affiliation: Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Porcelli
authors.affiliation: Department of Biomedical and NeuroMotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Serretti
authors.affiliation: University Psychiatric Clinic Ljubljana, Ljubljana, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Rus Makovec
authors.affiliation: University Psychiatric Clinic Ljubljana, Ljubljana, Slovenia.
authors.forename: Blanka
authors.initials: B
authors.lastname: Kores Plesnicar
authors.affiliation: Pharmacogenetics Laboratory, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia vita.dolzan@mf.uni-lj.si.
authors.forename: Vita
authors.initials: V
authors.lastname: Dolžan
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000235:genetics* / Q000523:psychology; D000483:Alleles; D001007:Anxiety / Q000235:genetics / Q000523:psychology; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009771:Obsessive-Compulsive Disorder / Q000235:genetics / Q000523:psychology; D020641:Polymorphism, Single Nucleotide; D012720:Severity of Illness Index; D017524:Slovenia; D055815:Young Adult
chemical_list: D002374:Catalase; D019392:Cytochrome P-450 CYP2E1