title: Modified Lipoproteins by Acrylamide Showed More Atherogenic Properties and Exposure of Acrylamide Induces Acute Hyperlipidemia and Fatty Liver Changes in Zebrafish.
abstract: Acrylamide is a well-known potent carcinogen and neurotoxin that, until now, has not been sufficiently investigated with regard to its effects on lipid metabolism. We investigated physiological effects of acrylamide (AA) on lipoprotein metabolism using human macrophages, dermal cells, and zebrafish models. Functional and structural properties of lipoproteins were modified by AA (final concentration of 5-100 mM) with loss of antioxidant ability and multimerization of apoA-I in vitro. AA exacerbated LDL oxidation, degradation, and LDL uptake into macrophages with increased ROS production. In human cells, treatment of AA (1-100 μM) caused cellular senescence of dermal cells with severe cytotoxicity. Waterborne exposure of zebrafish in cage water containing AA (300 ppm) resulted in acute death within 26 h along with elevation of body weight, blood glucose, triglyceride, and hepatic inflammation. AA exposure caused fat accumulation in liver in a dose-dependent manner. In conclusion, AA affected lipoprotein metabolism to result exacerbation of atherosclerosis. Exposure of zebrafish to AA resulted in acute inflammatory death with hyperlipidemia.
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Ji-Mi
authors.initials: JM
authors.lastname: Baek
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: So-Mang
authors.initials: SM
authors.lastname: Lim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Jae-Yong
authors.initials: JY
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Jihoe
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea.
authors.forename: Inho
authors.initials: I
authors.lastname: Choi
authors.affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. chok@yu.ac.kr.|Research Institute of Protein Sensor, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. chok@yu.ac.kr.|BK21plus Program Serum Biomedical Research and Education Team, Yeungnam University, Gyeongsan, 712-749, Republic of Korea. chok@yu.ac.kr.
authors.forename: Kyung-Hyun
authors.initials: KH
authors.lastname: Cho
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D016632:Apolipoprotein A-I / Q000097:blood; D050197:Atherosclerosis / Q000097:blood / Q000139:chemically induced*; D015415:Biomarkers / Q000097:blood; D045744:Cell Line, Tumor; D016922:Cellular Senescence / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005234:Fatty Liver / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000139:chemically induced*; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D013997:Time Factors; D015027:Zebrafish
chemical_list: C505693:APOA1 protein, human; D016632:Apolipoprotein A-I; D015415:Biomarkers; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D017382:Reactive Oxygen Species; C099252:oxidized low density lipoprotein; D020106:Acrylamide
keywords: Acrylamide; Atherosclerosis; Dermal cells; Lipoprotein; Senescence; Zebrafish