title: Surface plasmon resonance (SPR)-based biosensor technology for the quantitative characterization of protein-carotenoid interactions.
abstract: The surface plasmon resonance (SPR) biosensor method is a highly sensitive, label-free technique to study the non-covalent interactions of biomolecules, especially protein-protein and protein-small molecule interactions. We have explored this robust biosensor platform to study the interactions of carotenoid-binding proteins and their carotenoid ligands to assess the specificity of interaction, kinetics, affinity, and stoichiometry. These characterizations are important to further study uptake and transport of carotenoids to targeted tissues such as the macula of the human eye. In this review, we present an overview of the SPR method and optimization of assay conditions, and we discuss the particular challenges in studying carotenoid-protein interactions using SPR.
authors.affiliation: Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States.
authors.forename: Preejith P
authors.initials: PP
authors.lastname: Vachali
authors.affiliation: Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States.
authors.forename: Binxing
authors.initials: B
authors.lastname: Li
authors.affiliation: Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States.
authors.forename: Alexis
authors.initials: A
authors.lastname: Bartschi
authors.affiliation: Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States. Electronic address: paul.bernstein@hsc.utah.edu.
authors.forename: Paul S
authors.initials: PS
authors.lastname: Bernstein
mesh_terms: D001692:Biological Transport; D002338:Carotenoids / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D006801:Humans; D008266:Macula Lutea / Q000378:metabolism; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D002352:Carrier Proteins; D002338:Carotenoids
keywords: Age-related macular degeneration; Biosensor; Carotenoid-binding proteins; GSTP1; IRBP; Macula; Retina; StARD3; Xanthophylls