title: Marine antimicrobial peptide tachyplesin as an efficient nanocarrier for macromolecule delivery in plant and mammalian cells.
abstract: Membrane-active peptides can be classified as cell-penetrating peptides and antimicrobial peptides (AMPs) that are known to play interchangeable roles. In this study, this dual behaviour was studied for the marine AMP, tachyplesin. It is a well-established cyclic peptide known to possess antimicrobial properties and was investigated for its cell-penetrating property and cargo delivery ability. Because of its derivation from a marine organism as well as cyclic nature, it has been shown to possess higher stability in vitro. In this study, its internalization as a cell-penetrating peptide was established and characterized in both plant and mammalian systems. It was shown to deliver cargo molecules in both living systems, emerging as an efficient nonviral macromolecule nanocarrier.
authors.affiliation: Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, India.
authors.forename: Aastha
authors.initials: A
authors.lastname: Jain
authors.forename: Bhoopesh K
authors.initials: BK
authors.lastname: Yadav
authors.forename: Archana
authors.initials: A
authors.lastname: Chugh
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D029688:Brassica napus / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D046911:Macromolecular Substances / Q000378:metabolism; D018613:Microscopy, Confocal; D010456:Peptides, Cyclic / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D057846:Cell-Penetrating Peptides; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D010456:Peptides, Cyclic; C057636:tachyplesin peptide, Tachypleus tridentatus
keywords: cargo delivery; endocytosis; marine bioresources; stability; translocation