title: Endoplasmic reticulum stress is involved in the lidocaine-induced apoptosis in SH-SY5Y neuroblastoma cells.
abstract: Lidocaine has been indicated to promote apoptosis and to promote endoplasmic reticulum (ER) stress. However, the mechanism underlining ER stress-mediated apoptosis is unclear. In the present study, we investigated the promotion to ER stress in the lidocaine-induced apoptosis in human neuroblastoma SH-SY5Y cells. Firstly, we confirmed that lidocaine treatment induced apoptosis in SH-SY5Y cells, time-dependently and dose-dependently, via MTT cell viability assay and annexin V/FITC apoptosis detection with a FACScan flow cytometer. And the anti-apoptosis Bcl-2 and Bcl-xL were downregulated, whereas the apoptosis-executive caspase 3 was promoted through Western blot assay and caspase 3 activity assay. Moreover, the ER stress-associated binding immunoglobulin protein (BiP), PKR-like ER kinase (PERK), activating transcription factor 4 (ATF4) and CCAAT/enhancer-binding protein homologous protein (CHOP) were also upregulated at both mRNA and protein levels by lidocaine treatment. On the other hand, downregulation of the ER stress-associated BiP by RNAi method not only blocked the lidocaine-promoted ER stress but also attenuated the lidocaine-induced SH-SY5Y cell apoptosis. In conclusion, the present study confirmed the involvement of ER stress in the lidocaine-induced apoptosis in human neuroblastoma SH-SY5Y cells. Our study provides a better understanding on the mechanism of lidocaine's neurovirulence.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital of Henan Science and Technology University, No. 24 Jinghua Road, Jianxi District, 471003, Luoyang, China, khann_li@163.com.
authors.forename: Kehan
authors.initials: K
authors.lastname: Li
authors.forename: Xuechang
authors.initials: X
authors.lastname: Han
mesh_terms: D051701:Activating Transcription Factor 4 / Q000235:genetics / Q000378:metabolism; D000779:Anesthetics, Local / Q000494:pharmacology* / Q000633:toxicity; D017209:Apoptosis; D045744:Cell Line, Tumor; D059865:Endoplasmic Reticulum Stress; D006801:Humans; D008012:Lidocaine / Q000494:pharmacology* / Q000633:toxicity; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism; D019892:eIF-2 Kinase / Q000235:genetics / Q000378:metabolism
chemical_list: D051701:Activating Transcription Factor 4; D000779:Anesthetics, Local; D008012:Lidocaine; D051742:Transcription Factor CHOP; D019892:eIF-2 Kinase