title: [Effects of propranolol on proliferation of hemangioma-derived mesenchymal stem cells ].
abstract: OBJECTIVE
  To explore the new mechanism of propranolol for treatment of hemangioma and the effects of propranolol on proliferation of hemangioma-derived mesenchymal stem cells ( Hem- MSCs).
  
  
  METHODS
  We isolated Hem-MSCs from hemangioma in the proliferating phase by their selective adhesion to plastic culture dishes. Immunofluorescence staining was used to examine the expression of marker antigens in Hem-MSCs. Human umbilical vein endothelial cells(HUVECs) were used as control. Indiuction of multi-lineage differentiation including osteogenesis and adipogeneis was performed with appropriate medium to identify the multi-lineage differentiation potential. MTT cell counting was used to observe the effects of different concentrations of propranolol on proliferation of Hem-MSCs.
  
  
  RESULTS
  Hem- MSCs were fibroblast-like morphology. All of them expressed vimentin, most expressed α-SMA,CD133, some expressed Glutl, and none of them expressed VEGF. Osteogenic, adipogenic differentiations of Hem- MSCs were induced successfully. Effects of low concentration of propranolol on proliferation of Hem-MSCs were not obvious, while high concentration of propranolol can inhibit the proliferation of Hem-MSCs.
  
  
  CONCLUSIONS
  The cells we isolated from hemangioma are Hem-MSCs. High concentration of propranolol can inhibit the proliferation of Hem-MSCs.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Tinghui
authors.forename: Ma
authors.initials: M
authors.lastname: Xiaorong
authors.forename: Huang
authors.initials: H
authors.lastname: Yingying
authors.forename: Chen
authors.initials: C
authors.lastname: Huiping
authors.forename: Xiao
authors.initials: X
authors.lastname: Yan
authors.forename: Ouyang
authors.initials: O
authors.lastname: Tianxiang
mesh_terms: D050156:Adipogenesis; D000941:Antigens / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis; D011433:Propranolol / Q000494:pharmacology*; D014471:Umbilical Veins; D014746:Vimentin / Q000378:metabolism
chemical_list: D000941:Antigens; D014746:Vimentin; D011433:Propranolol