title: Brucella suis infection in domestic pigs in Sardinia (Italy).
abstract: During a 4-year (2007-2010) survey, the presence of Brucella suis infection in domestic pigs in Sardinia was investigated. Serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the Rose Bengal (RBT) and complement fixation (CFT) tests for screening and confirmation of Brucella, respectively. Of the 1251 serum samples analysed by RBT, 406 sera, originating from 36 farms, were positive for B. suis. CFT was positive in 292/748 sera analysed, confirming positivity in all 36 pig herds. Pigs with international complement fixation test units per ml (ICFTU/ml) values ⩾160 were slaughtered, and their organs collected for bacteriological examination and testing by polymerase chain reaction (PCR). Brucella spp. strains were isolated in culture from 13/502 organs analysed, and subsequently identified as B. suis biovar 2. PCR detected positivity to Brucella spp. in 19/285 organs analysed. These results confirm the presence and emergence of B. suis infection in domestic pigs in Sardinia.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna 'G. Pegreffi',Department of Cagliari,Elmas,Cagliari,Italy.
authors.forename: C
authors.initials: C
authors.lastname: Pilo
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna 'G. Pegreffi',Department of Cagliari,Elmas,Cagliari,Italy.
authors.forename: M T
authors.initials: MT
authors.lastname: Tedde
authors.affiliation: Dipartimento di Chirurgia e Scienze Odontostomatologiche,Università di Cagliari,Cagliari,Italy.
authors.forename: G
authors.initials: G
authors.lastname: Orrù
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna 'G. Pegreffi',Department of Cagliari,Elmas,Cagliari,Italy.
authors.forename: G
authors.initials: G
authors.lastname: Addis
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sardegna 'G. Pegreffi',Department of Cagliari,Elmas,Cagliari,Italy.
authors.forename: M
authors.initials: M
authors.lastname: Liciardi
mesh_terms: D000818:Animals; D041021:Brucella suis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D003168:Complement Fixation Tests; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D003625:Data Collection; D007558:Italy / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012395:Rose Bengal / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D012395:Rose Bengal
keywords: Brucella suis; Italy; Sardinia; domestic pig; epidemiology