title: Increased expression of fibroblast growth factor receptor 1 in endometriosis and its correlation with endometriosis-related dysmenorrhea and recurrence.
abstract: OBJECTIVE
  This study aims to identify a critical molecule that potentially participates in endometriosis pathogenesis and characterize its correlation with dysmenorrhea and recurrence.
  
  
  METHODS
  We utilized a bioinformatics-based strategy to screen for candidate genes and fibroblast growth factor receptor 1(FGFR1) was chosen for further validation. FGFR1 expression was examined in specimens of ectopic and eutopic endometrium obtained from 48 patients with endometriosis and specimens of eutopic endometrium from 26 healthy control subjects using immunohistochemistry and Western blotting. In addition, FGFR shRNA treatment was applied in a nude mice endometriosis model to examine the functional role of FGFR1 in endometriosis formation in vivo.
  
  
  RESULTS
  FGFR1 was found commonly overexpressed in ectopic endometrium of endometriosis compared with either its eutopic counterpart or endometrium from normal patients (P < 0.05). FGFR shRNA treatment impaired endometriosis formation and alleviated endometriosis-related symptoms in vivo. FGFR1 expression in ectopic endometrium was correlated with dysmenorrhea severity (P < 0.05) and recurrence in endometriosis patients (P < 0.05).
  
  
  CONCLUSIONS
  FGFR1 might be involved in endometriosis development, which could possibly serve as a novel therapeutic target and prognostic marker for this disease.
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Linjie
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Huiliang
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: College of Life Science, Sichuan University, Chengdu 610041, PR China.
authors.forename: Zhongyue
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Life Science, Sichuan University, Chengdu 610041, PR China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Jitong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ren
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China. Electronic address: taotaovip2005@163.com.
authors.forename: Shengtao
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Chengdu 610041, PR China; The State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D019295:Computational Biology; D004195:Disease Models, Animal; D004412:Dysmenorrhea / Q000209:etiology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004715:Endometriosis / Q000150:complications / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000235:genetics / Q000378:metabolism*; D012008:Recurrence
chemical_list: D051496:Receptor, Fibroblast Growth Factor, Type 1
keywords: Dysmenorrhea; Endometriosis; FGFR1; Recurrence