title: Identification of heat shock protein 27 as a novel autoantigen of Behçet's disease.
abstract: OBJECTIVE
  The aim of this study was to identify candidate pathogenic autoantigens of Behçet's disease (BD) in pathogen-stimulated target cells.
  
  
  METHODS
  First, three cell lines were used as target cells to screen autoantibody. Second, selected target cells were simulated with pathogens. Third, western blotting was used for detecting the auto-antigens in cell extracts. Next, immunoprecipitation was performed and the amino-acid sequences of target antigens were analyzed by LC-MALDI-TOF/TOF. Then, the potential target antigen was expressed, purified, and immunologically confirmed. And finally, an ELISA kit was developed and clinically validated through the assessments of 456 clinical samples with BD.
  
  
  RESULTS
  One antigen with a molecular weight of approximately 27-kDa was identified as heat shock protein 27 (HSP27). The reactivity of serum IgG against recombinant human HSP27 was detected in 52 of 91 BD patients (57%), 66 of 92 rheumatoid arthritis (RA) patients (72%), 32 of 90 Sjogren syndrome (SS) patients (36%), 22 of 92 systemic lupus erythematosus (SLE) patients (24%) and 0 of 91 healthy controls (HC). The reactivity of BD serum IgG antibodies against HSP27 was significantly higher than SLE (P<0.0001) SS (P<0.0001) and HC (P<0.0001).
  
  
  CONCLUSIONS
  This study identified HSP27 as a candidate endothelial cell autoantigen of BD, which is interesting and probably worth further exploration.
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Shi
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Ji
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Yan
authors.affiliation: Core Facility, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Shutao
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Xun
authors.affiliation: ImmunoHunt Corporation, 139 Fengtai Rd, Beijing 100071, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China.
authors.forename: Weiyang
authors.initials: W
authors.lastname: Chen
authors.affiliation: School of Chemistry and Biotechnology Engineering, University of Science & Technology Beijing, Beijing 100083, China. Electronic address: hongwudu@ustb.edu.cn.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D001528:Behcet Syndrome / Q000097:blood / Q000276:immunology*; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006360:Heat-Shock Proteins; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D015203:Reproducibility of Results; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D010455:Peptides
keywords: Autoantigen; Behçet’s disease; HSP27