title: [Proteomic analysis of peripheral blood mononuclear cells to identify potential markers of fibrosis in chronic hepatitis B].
abstract: OBJECTIVE
  To identify non-invasive biomarkers for diagnosis and/or prognosis of liver fibrosis in chronic hepatitis B (CHB).
  
  
  METHODS
  Peripheral blood samples were obtained from 48 patients with CHB, including 24 with mild fibrosis (stage 1, S1) and 24 with severe fibrosis (stage 4, S4), and subjected to Ficoll density gradient centrifugation in order to obtain enriched samples of peripheral blood mononuclear cells (PBMCs).The PBMC proteomes of the two groups were assessed by first separating the total proteins by two-dimensional gel electrophoresis (2DE) and then identifying the differentially expressed proteins by liquid chromatography combined with tandem mass spectrometry (LCMS/MS).
  
  
  RESULTS
  The enriched PBMC samples from the S1 group and the S4 group had similar amounts of platelets [(19.268+/- 6.413) * 109/L and(19.480+/- 6.538) * 109/L, respectively); however, for both, the platelet amounts were 5 to 15-fold lower than that of the normal reference (100-300 *109/L). There was no significant difference found between the platelet amounts in the S1 patients and healthy controls (P=0.930). Twelve differentially expressed proteins were identified through 2DE-LC-MS/MS, including proteins such as moesin and NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 that are involved in various biological processes like cell movement, cell adhesion, kinase signaling and transcription.
  
  
  CONCLUSIONS
  s The 12 proteins with differential expression in S1 and S4 patients with CHB and liver fibrosis may represent markers related to development and/or progression of liver fibrosis.
authors.affiliation: Shanghai Public Health Clinical Center, Shanghai 201508, China.
authors.forename: Jiao-Li
authors.initials: JL
authors.lastname: Zhang
authors.forename: Yong-Fu
authors.initials: YF
authors.lastname: Liu
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Jia
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Yin
authors.forename: Xiao-Qian
authors.initials: XQ
authors.lastname: Liu
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Lyu
authors.forename: Zhan-Qing
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Bao-Chi
authors.initials: BC
authors.lastname: Liu
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhang
mesh_terms: D015415:Biomarkers; D018450:Disease Progression; D015180:Electrophoresis, Gel, Two-Dimensional; D019694:Hepatitis B, Chronic / Q000150:complications*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D013058:Mass Spectrometry; D011379:Prognosis; D020543:Proteome; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D020543:Proteome