title: [Transmembrane transport behavior of in vitro HepG2 cells of ananas and its effect on lipids and glucose distribution].
abstract: Pineapple (Ananas comosus) leaves contain mainly phenolic components with antioxidant and hypolipidemic effects. One of the principle components is p-coumaric acid. In this study, the transport behavior of p-coumaric acid, was observed after the administration of pineapple leaf phenols in vitro. Simultaneously, the effect of the phenols on glucose, total cholesterol and triglycerides transportation and metabolism in HepG2 cells was also observed. The results showed that the phenols had good transport characteristics. 5 min after the administration, p-coumaric acid of the phenols could be detected, and the content of p-coumaric acid reached the peak concentration after 60 min of the administration. p-coumaric acid of phenols have time-and dose-dependent manner. While promoting glucose transporter (GLUT4) and low density lipoprotein receptor (LDLR) expression, the phenols decreased intracellular lipid content. This reduction of intracellular lipid content was highly correlated with the promotion of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) expression, while the reduction of intracellular glucose levels was correlated with glycogen synthesis in the cells.
authors.forename: Yu-Nong
authors.initials: YN
authors.lastname: Pang
authors.forename: Yu-Shuang
authors.initials: YS
authors.lastname: Chai
authors.forename: Jing-Fei
authors.initials: JF
authors.lastname: Jiang
authors.forename: Xin-Pei
authors.initials: XP
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Yu
authors.forename: Fan
authors.initials: F
authors.lastname: Lei
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Xing
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Du
mesh_terms: D031230:Ananas / Q000737:chemistry; D001692:Biological Transport / Q000187:drug effects; D002784:Cholesterol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D002784:Cholesterol; D005947:Glucose