title: Functional role of EMMPRIN in the formation and mineralisation of dental matrix in mouse molars.
abstract: Our previous research has shown that the extracellular matrix metalloproteinase inducer (EMMPRIN) is expressed during and may function in the early development of tooth germs. In the present study, we observed the specific expression of EMMPRIN in ameloblasts and odontoblasts during the middle and late stages of tooth germ development using immunohistochemistry. Furthermore, to extend our understanding of the function of EMMPRIN in odontogenesis, we used an anti-EMMPRIN function-blocking antibody to remove EMMPRIN activity in tooth germ culture in vitro. Both the formation and mineralisation of dental hard tissues were suppressed in the tooth germ culture after the abrogation of EMMPRIN. Meanwhile, significant reductions in VEGF, MMP-9, ALPL, ameloblastin, amelogenin and enamelin expression were observed in antibody-treated tooth germ explants compared to control and normal serum-treated explants. The current results illustrate that EMMPRIN may play a critical role in the processing and maturation of the dental matrix.
authors.affiliation: Department of Prosthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Guofang
authors.initials: G
authors.lastname: Xing
authors.forename: Liwen
authors.initials: L
authors.lastname: Hou
authors.forename: Jing
authors.initials: J
authors.lastname: Bao
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Jiao
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D051926:Basigin / Q000235:genetics* / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects / Q000235:genetics*; D015870:Gene Expression; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008963:Molar / Q000378:metabolism*; D009024:Morphogenesis / Q000187:drug effects / Q000235:genetics; D009805:Odontogenesis / Q000235:genetics; D014083:Tooth Germ / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D057134:Antibodies, Neutralizing; D042461:Vascular Endothelial Growth Factor A; D051926:Basigin; D020780:Matrix Metalloproteinase 9