title: Optimal time-points for minimal residual disease monitoring change on the basis of the method used in patients with acute myeloid leukemia who underwent allogeneic stem cell transplantation: a comparison between multiparameter flow cytometry and Wilms' tumor 1 expression.
abstract: Minimal residual disease (MRD) of 30 adult AML patients was monitored by multiparameter flow cytometry (MFC) and WT1 expression before and after allogeneic stem cell transplantation (allo-SCT). Diagnostic performance of pre-transplant MRD measured by MFC was higher than that obtained by WT1 expression. Comparable results were displayed at day +30 post-transplant, while better values by WT1 compared to MFC were found at day +90. Positive MRD by MFC predicted a shorter disease free survival (DFS) before and 1 month after transplant (p=0.006 and p=0.005), while only high WT1 levels at 1 month from the transplant significantly impacted on DFS (p=0.010). Our results support the idea that MRD monitoring by MFC should be suggested before and 30 days after the transplant, while WT1 expression should be preferred after this procedure. The assessment of MRD at day +30 from allo-SCT is recommended as post transplant check-point for the predictive role displayed, independently of the method used.
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy. Electronic address: giovannirossi.fr@gmail.com.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Rossi
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Angelo Michele
authors.initials: AM
authors.lastname: Carella
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Maria Marta
authors.initials: MM
authors.lastname: Minervini
authors.affiliation: Department of Hygiene, Catholic University of Sacred Heart, 00165 Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: di Nardo
authors.affiliation: Department of Hygiene, Catholic University of Sacred Heart, 00165 Rome, Italy.
authors.forename: Chiara de
authors.initials: Cd
authors.lastname: Waure
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Michele Mario
authors.initials: MM
authors.lastname: Greco
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Merla
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Giovanni Pio de
authors.initials: GP
authors.lastname: Cillis
authors.affiliation: Department of Hematology, "Vito Fazzi" Hospital, 73100 Lecce, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Di Renzo
authors.affiliation: Department of Hematology, "Perrino" Hospital, 72100 Brindisi, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Melpignano
authors.affiliation: Department of Hematology, "Riuniti" Hospital, 71100 Foggia, Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Capalbo
authors.affiliation: Department of Hematology, "Riuniti" Hospital, 71100 Foggia, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Palumbo
authors.affiliation: Department of Hematology, "San Giuseppe Moscati" Hospital, 74121 Taranto, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Pisapia
authors.affiliation: Department of Hematology and Stem Cell Transplant Unit, IRCCS "Casa Sollievo della Sofferenza" Hospital, 71013 San Giovanni Rotondo, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Cascavilla
mesh_terms: D000293:Adolescent; D000328:Adult; D064591:Allografts; D005260:Female; D005434:Flow Cytometry; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D018365:Neoplasm, Residual; D033581:Stem Cell Transplantation; D025721:WT1 Proteins / Q000096:biosynthesis*
chemical_list: D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Acute myeloid leukemia; Allogeneic stem cell transplantation; Minimal residual disease; Multiparameter flow cytometry; WT1 expression