title: Overexpression of PGC1α and accumulation of p62 in apocrine carcinoma of the breast.
abstract: Apocrine carcinoma is categorized as a special type of breast carcinoma because of its specific morphological features. To clarify the characteristics of apocrine carcinoma from the point of view of the mitochondrial profile, we conducted a comparative study between apocrine and non-apocrine carcinomas. The expressions of mitochondrial related factors (PGC1α, Nrf1, Nrf2, mtTFA and COX4) were examined in a testing set of breast cancer tissue. Apocrine carcinomas showed a clear tendency towards higher mRNA expression levels of PGC1α than non-apocrine carcinomas. The expression of the selected factor, PGC1α, as well as that of p62 was further examined. The results revealed that apocrine carcinomas showed a higher immunohistochemical positivity rate for PGC1α (21.3% vs. 3.2%; P = 0.008), and that the mRNA expression level of PGC1α was significantly higher in apocrine carcinoma than in non-apocrine carcinoma (P = 0.007). The immunohistochemical positivity rate for p62 protein was also higher in apocrine carcinomas (44.7% vs. 21.0%; P = 0.015), although no significant difference in the p62 mRNA expression level was detected between the two types of carcinoma (P = 0.633). In conclusion, this study revealed that apocrine carcinoma overexpressed PGC1α contributing to mitochondrial biogenesis, and also p62 protein accumulation.
authors.affiliation: Department of Pathology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Fuchinoue
authors.forename: Yukari
authors.initials: Y
authors.lastname: Hirotani
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakanishi
authors.forename: Hiromi
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Haruna
authors.initials: H
authors.lastname: Nishimaki
authors.forename: Hiroko
authors.initials: H
authors.lastname: Noda
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Tang
authors.forename: Misato
authors.initials: M
authors.lastname: Iizuka
authors.forename: Sadao
authors.initials: S
authors.lastname: Amano
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sugitani
authors.forename: Norimichi
authors.initials: N
authors.lastname: Nemoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Masuda
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D061346:Laser Capture Microdissection; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D016601:RNA-Binding Proteins / Q000096:biosynthesis*; D060888:Real-Time Polymerase Chain Reaction; D013544:Sweat Gland Neoplasms / Q000378:metabolism*; D046888:Tissue Array Analysis; D014157:Transcription Factors / Q000096:biosynthesis*; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: C118585:P62 protein, human; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D016601:RNA-Binding Proteins; D014157:Transcription Factors
keywords: PGC1 alpha; apocrine carcinoma; mitochondria; p62