title: IL-22 Up-Regulates β-Defensin-2 Expression in Human Alveolar Epithelium via STAT3 but Not NF-κB Signaling Pathway.
abstract: Human β-defensin-2(HBD-2) is one of the two major vertebrate antimicrobial peptide families (α and β), which is highly expressed by proinflammatory induction in the lung and exhibit broad-spectrum antimicrobial activity. We observed that IL-22 receptors high expressed on the membrane of A549 cells; HBD-2 mRNA was expressed in a time- and concentration-dependent manners in A549 cells when treated with IL-22; further studies demonstrated that HBD-2 expression was attenuated by AG490, but to JSH-23, inhibitors of p-STAT3 DNA binding and NF-κB/p65 subunit nuclear translocation, respectively. These results support that IL-22-mediated signalling pathway of HBD-2 gene expression involved STAT3 but not NF-κB in human alveolar epithelium. These findings provide a new insight into how IL-22 may play an important link between innate and adaptive immunity, thereby anti-infection locally in the alveolar epithelium.
authors.affiliation: Department of Respiratory Medicine, The Fourth Affiliated Hospital of Bengbu Medical College, Huainan, 232000, Anhui, China.
authors.forename: Amin
authors.initials: A
authors.lastname: Li
authors.forename: Yuying
authors.initials: Y
authors.lastname: Gan
authors.forename: Ruikai
authors.initials: R
authors.lastname: Wang
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Liu
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Mao
authors.initials: M
authors.lastname: Huang
authors.forename: Xuefan
authors.initials: X
authors.lastname: Cui
mesh_terms: D002460:Cell Line; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors; D004847:Epithelial Cells / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007378:Interleukins / Q000378:metabolism*; D010655:Phenylenediamines / Q000494:pharmacology; D011650:Pulmonary Alveoli / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000378:metabolism; D020545:Respiratory Mucosa / Q000276:immunology*; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000378:metabolism; D020032:Tyrphostins / Q000494:pharmacology; D023083:beta-Defensins / Q000096:biosynthesis / Q000378:metabolism*; D000097043:Interleukin-22
chemical_list: D004268:DNA-Binding Proteins; D007378:Interleukins; D010655:Phenylenediamines; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D050796:STAT3 Transcription Factor; D051996:Transcription Factor RelA; D020032:Tyrphostins; D023083:beta-Defensins; D000097043:Interleukin-22; C549066:4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine; C106641:DEFB4A protein, human; C498143:RELA protein, human; C494086:STAT3 protein, human; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide