title: Diagnosis of the Lelystad strain of Porcine reproductive and respiratory syndrome virus infection in individually housed pigs: comparison between serum and oral fluid samples for viral nucleic acid and antibody detection.
abstract: There has been a developing interest in the use of oral fluid for the diagnosis of different pathogens such as Porcine reproductive and respiratory syndrome virus (PRRSV). PRRSV and PRRSV-specific antibodies have been shown to be present in oral fluid samples, but the correlation between diagnostic results in oral fluid and serum samples has been insufficiently addressed. Studies investigating this correlation focused on boars older than 6 months and type 2 strains, but it is known that the outcome of a PRRSV infection is age and strain dependent. To address this gap, the current study reports on the detection of PRRSV and PRRSV-specific antibodies in serum and oral fluid samples collected over a 6-week period after an experimental infection of 8-week-old individually housed pigs with Lelystad virus, the type 1 prototype strain. Quantitative reverse transcription polymerase chain reaction analysis showed that significantly more serum samples were PRRSV RNA-positive than oral fluid until 5 days postinfection (dpi). Between 7 and 21 dpi, PRRSV RNA detection was similar in both samples but higher detection rates in oral fluid were found from 28 dpi. Compared with existing literature, this highlights that detection rates at particular time points postinfection might vary in function of strain virulence and animal age and provides useful information for the interpretation of pen-based oral fluid results. An excellent agreement between the oral fluid and serum enzyme-linked immunosorbent assay results was observed at every time point, further supporting the usefulness of oral fluid as a diagnostic sample for antibody detection.
authors.affiliation: Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, CODA-CERVA, Ukkel, Belgium (Decorte, Cay, De Regge)Experimental Centre, CODA-CERVA, Machelen, Belgium (Van Campe, Mostin).
authors.forename: Inge
authors.initials: I
authors.lastname: Decorte
authors.affiliation: Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, CODA-CERVA, Ukkel, Belgium (Decorte, Cay, De Regge)Experimental Centre, CODA-CERVA, Machelen, Belgium (Van Campe, Mostin).
authors.forename: Willem
authors.initials: W
authors.lastname: Van Campe
authors.affiliation: Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, CODA-CERVA, Ukkel, Belgium (Decorte, Cay, De Regge)Experimental Centre, CODA-CERVA, Machelen, Belgium (Van Campe, Mostin).
authors.forename: Laurent
authors.initials: L
authors.lastname: Mostin
authors.affiliation: Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, CODA-CERVA, Ukkel, Belgium (Decorte, Cay, De Regge)Experimental Centre, CODA-CERVA, Machelen, Belgium (Van Campe, Mostin).
authors.forename: Ann Brigitte
authors.initials: AB
authors.lastname: Cay
authors.affiliation: Operational Direction Viral Diseases, Enzootic and (Re)emerging Diseases, CODA-CERVA, Ukkel, Belgium (Decorte, Cay, De Regge)Experimental Centre, CODA-CERVA, Machelen, Belgium (Van Campe, Mostin) nick.deregge@coda-cerva.be.
authors.forename: Nick
authors.initials: N
authors.lastname: De Regge
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis* / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006799:Housing, Animal; D009055:Mouth / Q000821:virology*; D019318:Porcine Reproductive and Respiratory Syndrome / Q000097:blood / Q000175:diagnosis* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000145:classification / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine
chemical_list: D000914:Antibodies, Viral
keywords: Enzyme-linked immunosorbent assay; Lelystad strain; Porcine reproductive and respiratory syndrome virus; oral fluid; quantitative reverse transcription polymerase chain reaction