title: Genetic characterization of Toxoplasma gondii isolates from eared doves (Zenaida auriculata) in Brazil.
abstract: Eared doves (Zenaida auriculata), which are common in urban, rural and wild areas in many regions of Brazil, are frequently prey for domestic cats. Therefore Toxoplasma gondii isolates obtained from doves may reflect greater environmental diversity than those from other hosts. The aim of the present study was to evaluate T. gondii seroprevalence, isolate and genotype strains from Z. auriculata. Serum and tissue samples were collected from 206 doves for use in the modified agglutination test (MAT) and mouse bioassay. The prevalence of T. gondii antibodies in the doves was 22.3% (46/206), with titers ranging from 16 to 4096, and T. gondii strains were isolated from 12 of these doves. Five genotypes were detected by means of PCR-RFLP, including ToxoDB genotypes #1, #6, #17 and #65, and one genotype that had not previously been described (ToxoDB#182). This was the first report on isolation of T. gondii from Z. auriculata. This study confirmed the genetic diversity of T. gondii isolates and the existence of clonal type II (ToxoDB genotype #1) in Brazil.
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Luiz Daniel de
authors.initials: LD
authors.lastname: Barros
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Taroda
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Dauton Luiz
authors.initials: DL
authors.lastname: Zulpo
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Ivo Alexandre Leme da
authors.initials: IA
authors.lastname: Cunha
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Ana Sue
authors.initials: AS
authors.lastname: Sammi
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Sérgio Tosi
authors.initials: ST
authors.lastname: Cardim
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Miura
authors.affiliation: Department of Microbiology, The University of Tennessee, Knoxville, TN, USA.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Su
authors.affiliation: Department of Veterinary Pathology, School of Agrarian and Veterinary Sciences, Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, Jaboticabal, SP, Brazil.
authors.forename: Rosangela Zacarias
authors.initials: RZ
authors.lastname: Machado
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: Odilon
authors.initials: O
authors.lastname: Vidotto
authors.affiliation: Protozoology Laboratory, Department of Preventive Veterinary Medicine, Universidade Estadual de Londrina - UEL, Londrina, PR, Brazil.
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D001715:Bird Diseases / Q000453:epidemiology / Q000469:parasitology*; D001938:Brazil / Q000453:epidemiology; D010856:Columbidae / Q000097:blood* / Q000469:parasitology*; D005260:Female; D008297:Male; D016036:Seroepidemiologic Studies; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D014124:Toxoplasmosis, Animal / Q000097:blood* / Q000453:epidemiology
chemical_list: D000913:Antibodies, Protozoan