title: Systemic LPS and inflammatory response during consecutive days of exercise in heat.
abstract: This investigation studied circulating LPS activity, potential intestinal damage, and the systemic inflammatory response (SIR) during the exercise heat acclimation process. 8 healthy males (Age=24±3 years) ran in a hot environment on 5 consecutive days until core temperature (Tc) was elevated 2°C above rest. Plasma was obtained pre-, post-, 1 h post-, and 3 h post-exercise on the 1(st), 3(rd), and 5(th) day of exercise and analyzed for TNF-α, IL-6, IL-10, IL-1ra, LPS, and intestinal fatty acid-binding protein (I-FABP). Plasma LPS (1.1 EU·ml(-1)±0.1 vs. 0.7 EU·ml(-1)±0.03; P<0.01) and I-FABP (930.7 pg·ml(-1)±149.0 vs. 640.2 pg·ml(-1)±125.0; P<0.001) were significantly increased post-exercise each. The SIR remained largely unchanged during the study except for TNF-α. Plasma TNF-α was significantly lower on Day 5 at 1 h (3.2 pg·ml(-1)±0.6 vs. 4.5 pg·ml(-1)±0.8; P=0.01) and 3 h (3.6 pg·ml(-1)±0.8 vs. 4.8 pg·ml(-1)±0.9; P=0.05) post-exercise as compared to Day 1. Findings indicate that adaptations to exercise in the heat resulting in reductions of intestinal damage and plasma LPS activity require longer time periods in moderately trained males.
authors.affiliation: Children's National Medical Center, Center for Genetic Medicine Research, Washington, D.C., United States.
authors.forename: M D
authors.initials: MD
authors.lastname: Barberio
authors.affiliation: Department of Kinesiology, Berry College, Mount Berry, GA, United States.
authors.forename: D J
authors.initials: DJ
authors.lastname: Elmer
authors.affiliation: Children's National Medical Center, Center for Genetic Medicine Research, Washington, D.C., United States.
authors.forename: R H
authors.initials: RH
authors.lastname: Laird
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, United States.
authors.forename: K A
authors.initials: KA
authors.lastname: Lee
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, United States.
authors.forename: B
authors.initials: B
authors.lastname: Gladden
authors.affiliation: School of Kinesiology, Auburn University, Auburn, AL, United States.
authors.forename: D D
authors.initials: DD
authors.lastname: Pascoe
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D001810:Blood Volume; D001833:Body Temperature Regulation; D016207:Cytokines / Q000097:blood; D015444:Exercise / Q000502:physiology*; D050556:Fatty Acid-Binding Proteins / Q000097:blood*; D006339:Heart Rate; D006358:Hot Temperature; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000097:blood*; D008297:Male; D012420:Running / Q000502:physiology; D055815:Young Adult
chemical_list: D016207:Cytokines; D050556:Fatty Acid-Binding Proteins; D008070:Lipopolysaccharides