title: [Effects of dopamine and adenosine on regulation of water-electrolyte exchange in Amoeba proteus].
abstract: Dopamine and adenosine both regulate transport of sodium chloride in the renal tubules in mammals. We have studied the effect of dopamine and adenosine on spontaneous activity of contractile vacuole of Amoeba proteous. Both substances stimulated contractile vacuole. The effect of dopamine was suppressed by D2 receptor antagonist, haloperidol, but not by D1 antagonist, SCH 39166. Adenylate cyclase inhibitor, 2.5-dideoxyadenosine, suppressed the effect of dopamine, but not of adenosine. Inhibitor of protein kinase C, staurosporine, in contrast, blocked the effect of adenosine, but not dopamine. Notably, dopamine opposed effect of adenosine and vice versa. These results suggest that similar effects of dopamine and adenosine could be mediated by different intracellulare mechanisms.
authors.forename: Ia Iu
authors.initials: IaIu
authors.lastname: Bagrov
authors.forename: N B
authors.initials: NB
authors.lastname: Manusova
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000262:Adenylyl Cyclases / Q000378:metabolism; D000656:Amoeba / Q000187:drug effects* / Q000378:metabolism; D001552:Benzazepines / Q000494:pharmacology; D001692:Biological Transport; D016048:Dideoxyadenosine / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D065127:Dopamine D2 Receptor Antagonists / Q000494:pharmacology; D006220:Haloperidol / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D019311:Staurosporine / Q000494:pharmacology; D014617:Vacuoles / Q000187:drug effects* / Q000378:metabolism; D014882:Water-Electrolyte Balance / Q000187:drug effects* / Q000502:physiology
chemical_list: D001552:Benzazepines; D065127:Dopamine D2 Receptor Antagonists; D047428:Protein Kinase Inhibitors; D017448:Receptors, Dopamine D2; C058081:ecopipam; D016048:Dideoxyadenosine; D011493:Protein Kinase C; D000262:Adenylyl Cyclases; D019311:Staurosporine; D006220:Haloperidol; D000241:Adenosine; D004298:Dopamine