title: Inhibitory effect of Moutan Cortex aqueous fraction on mast cell-mediated allergic inflammation.
abstract: Moutan Cortex and its major compounds have been shown to possess various biological activities, including anti-inflammatory properties. However, the effects of Moutan Cortex aqueous fraction (MCA) and its molecular mechanisms have yet to be elucidated. In this study, we attempted to evaluate the effects of MCA on mast cell-mediated allergy inflammation in vitro and in vivo compared with major Moutan Cortex compounds. Thus, we examined the anti-inflammatory effects of a water extract of Moutan Cortex by comparing the inhibition of β-hexosaminadase and tumor necrosis factor-α (TNF-α) release in an aqueous fraction with other major compounds of Moutan Cortex. The inhibitory mechanism of MCA was investigated by western blotting in IgE-mediated DNP-BSA-stimulated RBL-2H3 cells. We confirmed the pharmacological effects of MCA on compound 48/80-induced allergic reactions in a mouse model by assessing scratching behavior and passive cutaneous anaphylaxis (PCA)-like reaction. Consequently, MCA inhibited IgE-mediated DNP-BSA-induced β-hexosaminadase and TNF-α release via inactivation of p38, ERK, Akt, and NF-κB in RBL-2H3 cells. MCA reduced compound 48/80-induced PCA reaction and scratching behavior in mice. This inhibitory effect of MCA is more potent than major compounds of Moutan Cortex. In conclusion, our results suggest that MCA has more potential in the treatment of allergic inflammatory diseases compared to other major compounds of Moutan Cortex.
authors.affiliation: Division of Pathogenic Biochemistry, Graduate School Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, Japan.
authors.forename: Ji-Ye
authors.initials: JY
authors.lastname: Kee
authors.forename: Akiko
authors.initials: A
authors.lastname: Inujima
authors.forename: Tsugunobu
authors.initials: T
authors.lastname: Andoh
authors.forename: Ken
authors.initials: K
authors.lastname: Tanaka
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Kuraishi
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sakurai
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Shibahara
authors.forename: Ikuo
authors.initials: I
authors.lastname: Saiki
authors.forename: Keiichi
authors.initials: K
authors.lastname: Koizumi
mesh_terms: D000818:Animals; D002417:Cattle; D004140:Dinitrophenols; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D006967:Hypersensitivity / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007073:Immunoglobulin E / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D008407:Mast Cells; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism*; D029596:Paeonia; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats; D012710:Serum Albumin, Bovine; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine
chemical_list: D004140:Dinitrophenols; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C034638:dinitrophenyl-bovine serum albumin; C038881:moutan cortex; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine; D011494:Protein Kinases