title: FcRγ promotes contact hypersensitivity to oxazolone without affecting the contact sensitisation process in B6 mice.
abstract: The process of sensitisation by specific contact allergens is indispensable for the induction of allergic contact dermatitis. Oxazolone is a well-characterised contact allergen. Previous studies suggested that immune cells bearing the FcRγ subunit are essential for oxazolone-induced contact hypersensitivity, but the biological functions of the FcRγ subunit in the process of sensitisation to oxazolone remain unknown. In this study, we show that FcRγ deficiency decreases ear-swelling responses to oxazolone in mice. However, we found that oxazolone-sensitised FcRγ(-/-) mice and oxazolone-sensitised wild-type (WT) mice have comparable numbers of CD11c(+) MHCII(hi) dendritic cells (DCs) in their draining lymph nodes (LNs). In addition, oxazolone-sensitised LN cells from both FcRγ(-/-) and WT mice showed considerable production of interferon-gamma (IFNγ), interleukin-4 (IL-4) and IL-17A upon oxazolone-keyhole limpet haemocyanin loading. Consistent with these data, oxazolone-sensitised FcRγ(-/-) and FcRγ(+/+) LN cells conferred contact hypersensitivity to WT naïve mice challenged with the hapten. Our findings clearly indicate that, in an experimental mouse model, the FcRγ subunit positively regulates contact hypersensitivity to oxazolone without affecting the contact sensitisation process.
authors.affiliation: Department of Dermatology, Nihon University School of Medicine, Tokyo, Japan; Allergy and Immunology Group, Research Institute of Medical Science, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nunomura
authors.forename: Mino
authors.initials: M
authors.lastname: Ohtsubo-Yoshioka
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Okayama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Terui
authors.forename: Chisei
authors.initials: C
authors.lastname: Ra
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D003713:Dendritic Cells; D017449:Dermatitis, Allergic Contact / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D003877:Dermatitis, Contact / Q000235:genetics / Q000276:immunology; D004137:Dinitrochlorobenzene; D007073:Immunoglobulin E / Q000097:blood / Q000187:drug effects; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010081:Oxazolone; D017455:Receptors, IgE / Q000008:administration & dosage / Q000276:immunology*; D017452:Receptors, IgG / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D004137:Dinitrochlorobenzene; C579617:FcepsilonRIalpha protein, mouse; D017455:Receptors, IgE; D017452:Receptors, IgG; D010081:Oxazolone; D007073:Immunoglobulin E
keywords: FcRγ; IgE; contact hypersensitivity