title: An integrated assessment of histopathological changes of the enteric neuromuscular compartment in experimental colitis.
abstract: Bowel inflammatory fibrosis has been largely investigated, but an integrated assessment of remodelling in inflamed colon is lacking. This study evaluated tissue and cellular changes occurring in colonic wall upon induction of colitis, with a focus on neuromuscular compartment. Colitis was elicited in rats by 2,4-dinitrobenzenesulfonic acid (DNBS). After 6 and 21 days, the following parameters were assessed on paraffin sections from colonic samples: tissue injury and inflammatory infiltration by histology; collagen and elastic fibres by histochemistry; HuC/D, glial fibrillar acidic protein (GFAP), proliferating cell nuclear antigen (PCNA), nestin, substance P (SP), von Willebrand factor, c-Kit and transmembrane 16A/Anoctamin1 (TMEM16A/ANO1) by immunohistochemistry. TMEM16A/ANO1 was also examined in isolated colonic smooth muscle cells (ICSMCs). On day 6, inflammatory alterations and fibrosis were present in DNBS-treated rats; colonic wall thickening and fibrotic remodelling were evident on day 21. Colitis was associated with both an increase in collagen fibres and a decrease in elastic fibres. Moreover, the neuromuscular compartment of inflamed colon displayed a significant decrease in neuron density and increase in GFAP/PCNA-positive glia of myenteric ganglia, enhanced expression of neural SP, blood vessel remodelling, reduced c-Kit- and TMEM16A/ANO1-positive interstitial cells of Cajal (ICCs), as well as an increase in TMEM16A/ANO1 expression in muscle tissues and ICSMCs. The present findings provide an integrated view of the inflammatory and fibrotic processes occurring in the colonic neuromuscular compartment of rats with DNBS-induced colitis. These morphological alterations may represent a suitable basis for understanding early pathophysiological events related to bowel inflammatory fibrosis.
authors.affiliation: Unit of Histology, Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Ippolito
authors.forename: Cristina
authors.initials: C
authors.lastname: Segnani
authors.forename: Mariella
authors.initials: M
authors.lastname: Errede
authors.forename: Daniela
authors.initials: D
authors.lastname: Virgintino
authors.forename: Rocchina
authors.initials: R
authors.lastname: Colucci
authors.forename: Matteo
authors.initials: M
authors.lastname: Fornai
authors.forename: Luca
authors.initials: L
authors.lastname: Antonioli
authors.forename: Corrado
authors.initials: C
authors.lastname: Blandizzi
authors.forename: Amelio
authors.initials: A
authors.lastname: Dolfi
authors.forename: Nunzia
authors.initials: N
authors.lastname: Bernardini
mesh_terms: D000818:Animals; D003092:Colitis / Q000473:pathology*; D003106:Colon / Q000473:pathology; D007249:Inflammation / Q000473:pathology; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
keywords: DNBS; colonic inflammatory fibrosis; myenteric ganglia; neuromuscular compartment; wall remodelling