title: Molecular genetic variability of commercial and wild accessions of passion fruit (Passiflora spp.) targeting ex situ conservation and breeding.
abstract: Passiflora species are distributed throughout Latin America, and Brazil and Colombia serve as the centers of diversity for this genus. We performed cross-species amplification to evaluate 109 microsatellite loci in 14 Passiflora species and estimated the diversity and genetic structure of Passiflora cincinnata, Passiflora setaceae and Passiflora edulis. A total of 127 accessions, including 85 accessions of P. edulis, a commercial species, and 42 accessions of 13 wild species, were examined. The cross-species amplification was effective for obtaining microsatellite loci (average cross-amplification of 70%). The average number of alleles per locus (five) was relatively low, and the average diversity ranged from 0.52 in P. cincinnata to 0.32 in P. setacea. The Bayesian analyses indicated that the P. cincinnata and P. setacea accessions were distributed into two groups, and the P. edulis accessions were distributed into five groups. Private alleles were identified, and suggestions for core collections are presented. Further collections are necessary, and the information generated may be useful for breeding and conservation.
authors.affiliation: Laboratory of Applied Molecular Genetics, Department of Exact and Natural Sciences, State University of Southwest Bahia, Itapetinga 45700-000, Brazil. csilva@uesb.edu.br.
authors.forename: Carlos Bernard M
authors.initials: CB
authors.lastname: Cerqueira-Silva
authors.affiliation: Laboratory of Applied Molecular Genetics, Department of Exact and Natural Sciences, State University of Southwest Bahia, Itapetinga 45700-000, Brazil. elisasantosuesb@gmail.com.
authors.forename: Elisa S L
authors.initials: ES
authors.lastname: Santos
authors.affiliation: Brazilian Agricultural Research Corporation, Cassava & Fruits, Cruz das Almas 44380-000, Brazil. onildo.nunes@embrapa.br.
authors.forename: Onildo N
authors.initials: ON
authors.lastname: Jesus
authors.affiliation: Molecular Biology and Genetic Engineering Center, University of Campinas, CP 6010 Campinas, Campinas 13083-875, Brazil. jgpvieira@gmail.com.
authors.forename: João G P
authors.initials: JG
authors.lastname: Vieira
authors.affiliation: Molecular Biology and Genetic Engineering Center, University of Campinas, CP 6010 Campinas, Campinas 13083-875, Brazil. gumori@gmail.com.
authors.forename: Gustavo M
authors.initials: GM
authors.lastname: Mori
authors.affiliation: Biotechnology and Genetic Center, Biological Sciences Department, State University of Santa Cruz, Ilhéus 45662-900, Brazil. ronanxc@uesc.br.
authors.forename: Ronan X
authors.initials: RX
authors.lastname: Corrêa
authors.affiliation: Molecular Biology and Genetic Engineering Center, University of Campinas, CP 6010 Campinas, Campinas 13083-875, Brazil. anete@unicamp.br.
authors.forename: Anete P
authors.initials: AP
authors.lastname: Souza
mesh_terms: D000483:Alleles; D001499:Bayes Theorem; D001947:Breeding; D016000:Cluster Analysis; D005638:Fruit; D005784:Gene Amplification; D014644:Genetic Variation; D018895:Microsatellite Repeats; D029598:Passiflora / Q000145:classification / Q000235:genetics*; D011110:Polymorphism, Genetic; D013045:Species Specificity