title: Determination of alditols by capillary electrophoresis with indirect laser-induced fluorescence detection.
abstract: A novel method was developed based on capillary electrophoresis with indirect laser-induced fluorescence detection for the determination of alditols including xylitol, sorbitol, arabinitol and mannitol. The baseline separation was achieved with a capillary with 30 μm inner diameter and running buffer (pH 9.6) composed of 40 mM sodium tetraborate, 5% (v/v) methanol, 10(-5)M sodium fluorescein as background fluorescence. Calibration curves (0.2-2.0 mg mL(-1)) showed good linear correlations (r>0.999). The detection limits of the alditols were in the range of 19.0-24.4 μg mL(-1). The intra-day and inter-day RSDs for migration times and peak areas were less than 1.6% and 4.3%, respectively. The average recoveries were in the range of 99.6-105.5%. The developed method was successfully applied to determination of alditols in different real samples, and the results were compared with those of capillary electrophoresis with indirect ultraviolet detection method.
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Ying
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: yuxiuxiao2011@whu.edu.cn.
authors.forename: Yuxiu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Pharmacy, South Central University for Nationalities, Wuhan 430074, China. Electronic address: meizhinan@163.com.
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Mei
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D013402:Sugar Alcohols / Q000032:analysis*
chemical_list: D013402:Sugar Alcohols
keywords: Alditols; Capillary electrophoresis; Indirect laser-induced fluorescence