title: Identification of biomarkers in cerebrospinal fluid and serum of multiple sclerosis patients by immunoproteomics approach.
abstract: Multiple sclerosis (MS) is an autoimmune inflammatory demyelinating disease of the central nervous system. At present, the molecular mechanisms causing the initiation, development and progression of MS are poorly understood, and no reliable proteinaceous disease markers are available. In this study, we used an immunoproteomics approach to identify autoreactive antibodies in the cerebrospinal fluid of MS patients to use as candidate markers with potential diagnostic value. We identified an autoreactive anti-transferrin antibody that may have a potential link with the development and progression of MS. We found this antibody at high levels also in the serum of MS patients and created an immunoenzymatic assay to detect it. Because of the complexity and heterogeneity of multiple sclerosis, it is difficult to find a single marker for all of the processes involved in the origin and progression of the disease, so the development of a panel of biomarkers is desirable, and anti-transferrin antibody could be one of these.
authors.affiliation: Istituto di Biomedicina e Immunologia Molecolare "A. Monroy", Consiglio Nazionale delle Ricerche (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy. paolocolomba@gmail.com.
authors.forename: Paolo
authors.initials: P
authors.lastname: Colomba
authors.affiliation: Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sez. Biologia e Genetica, Università di Palermo, via Divisi 83, 90139 Palermo, Italy. simona.fontana@unipa.it.
authors.forename: Simona
authors.initials: S
authors.lastname: Fontana
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze, Università di Palermo, via del Vespro 129, 90127 Palermo, Italy. giuseppe.salemi@unipa.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Salemi
authors.affiliation: Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sez. Biologia e Genetica, Università di Palermo, via Divisi 83, 90139 Palermo, Italy. marilisabarranca@yahoo.it.
authors.forename: Marilisa
authors.initials: M
authors.lastname: Barranca
authors.affiliation: Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sez. Biologia e Genetica, Università di Palermo, via Divisi 83, 90139 Palermo, Italy. claudia.losicco@gmail.com.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lo Sicco
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze, Università di Palermo, via del Vespro 129, 90127 Palermo, Italy. mariaantonietta.mazzola@gmail.com.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Mazzola
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze, Università di Palermo, via del Vespro 129, 90127 Palermo, Italy. paolo.ragonese@unipa.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Ragonese
authors.affiliation: Dipartimento di Biomedicina Sperimentale e Neuroscienze, Università di Palermo, via del Vespro 129, 90127 Palermo, Italy. giovanni.savettieri@unipa.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Savettieri
authors.affiliation: Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Sez. Biologia e Genetica, Università di Palermo, via Divisi 83, 90139 Palermo, Italy. giacomo.deleo@unipa.it.
authors.forename: Giacomo
authors.initials: G
authors.lastname: De Leo
authors.affiliation: Istituto di Biomedicina e Immunologia Molecolare "A. Monroy", Consiglio Nazionale delle Ricerche (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy. riccardo.alessandro@unipa.it.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Alessandro
authors.affiliation: Istituto di Biomedicina e Immunologia Molecolare "A. Monroy", Consiglio Nazionale delle Ricerche (CNR), Via Ugo La Malfa 153, 90146 Palermo, Italy. giovanni.duro@ibim.cnr.it.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Duro
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000134:cerebrospinal fluid; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007162:Immunoproteins / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid*; D040901:Proteomics / Q000379:methods; D014168:Transferrin / Q000276:immunology
chemical_list: D015415:Biomarkers; D007162:Immunoproteins; D014168:Transferrin