title: [Effects and clinical significance of virus load on red blood cell parameters in different stage of hepatitis B].
abstract: OBJECTIVE
  To evaluate the effect of the viral load on the red blood cell parameters in chronic hepatitis B patients and its clinical significance.
  
  
  METHODS
  In the study, 373 chronic hepatitis B patients were recruited, including 123 alanine transaminase (ALT) normal patients (ALT<40 U/L),128 ALT greater than or equal to the upper limit of normal, and less than 2 times higher than the upper limit of normal patients(40 U/L ≤ALT<80 U/L), and 122 ALT greater than or equal to 2 times higher than the upper limit of normal patients (ALT≥80 U/L). The blood routine parameters were measured by automatic blood cell counter. The liver function parameters were measured by automatic biochemical analyzer, the hepatitis B virus loads were measured by quantitative PCR analyzer and the results were analyzed by covariance analysis.
  
  
  RESULTS
  In the ALT normal chronic hepatitis B patients group, the viral load had minor effects on the red blood cell parameters.But in the ALT abnormal chronic hepatitis B patients group, the viral load had a significant effect on the red blood cell parameters, and the effect was most manifest in the ALT≥ double upper limit of normal group. The specific performance was that with the viral load increasing, the red blood cell [low copies group (4.10±0.67)×10(12)/L,medium copies group (3.92±0.69)×10(12)/L,high copies group (3.54±0.90) ×10(12)/L], the hemoglobin[low copies group (129.66±21.12 ) g/L, medium copies group (126.23±23.38) g/L, high copies group (112.98±27.77) g/L], the hematocrit (low copies group 37.66±5.68, medium copies group 37.03±6.03, high copies group 33.34±8.15) decreased(P=0.006,0.007,0.010),the mean corpuscular volume [low copies group (92.17±6.53) fL, medium copies group (94.85±7.95) fL, high copies group (101.63±11.33) fL], the mean corpuscular hemoglobin [low copies group (31.70±2.22) pg, medium copies group (33.11±3.62) pg, high copies group (34.65±3.13) pg], the mean corpuscular hemoglobin concentration [low copies group (344.28±17.17) g/L, medium copies group (351.33±16.90) g/L, high copies group (358.12±15.67) g/L], and the red blood cell distribution width-standard deviation [low copies group (52.49±9.04) fL, medium copies group (56.96±7.19) fL, high copies group (61.23 ±7.23) fL] increased(P=0.000,0.000,0.002,0.000).
  
  
  CONCLUSIONS
  Observing the effect of the viral load on the red blood cell parameters in chronic hepatitis B patients can reflect the effect of hepatitis B virus on the immune response and liver function in the different pathological stages, providing theoretical support for the clinical antiviral treatment.
authors.affiliation: Institute of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: P
authors.initials: P
authors.lastname: Gao
authors.affiliation: Institute of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: P
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Institute of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Yang
authors.affiliation: Institute of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Q F
authors.initials: QF
authors.lastname: Chen
authors.affiliation: Department of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: X R
authors.initials: XR
authors.lastname: Mao
authors.affiliation: Department of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Z B
authors.initials: ZB
authors.lastname: Zhao
authors.affiliation: Clinical laboratory, Second Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: L
authors.initials: L
authors.lastname: Shi
authors.affiliation: Clinical Laboratory, Jingchuan County People's Hospital, Gansu Jingchuan 744300, China.
authors.forename: L Z
authors.initials: LZ
authors.lastname: Yang
authors.affiliation: Institute of Infection, First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhou
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D004912:Erythrocytes / Q000821:virology*; D006515:Hepatitis B virus; D019694:Hepatitis B, Chronic / Q000097:blood*; D006801:Humans; D019562:Viral Load
chemical_list: D000410:Alanine Transaminase