title: Phenolic content, antioxidant and astroprotective response to oxidative stress of ethanolic extracts of Mentha longifolia from Sinai.
abstract: The aerial parts ofMentha longifolia L. are used as herbal remedies for curing different diseases through traditional Bedouin medicine. The antioxidant activity of the ethanolic extracts of M longifolia was investigated measuring peroxyl radical-scavenging activity by ORAC assay, with Trolox (a water-soluble analogue of α-tocopherol) employed as reference compound. In addition, the total content of phenolic compounds estimated by the Folin-Ciocalteau method and the identification of the polyphenols using high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) have been performed. Furthermore, the effect of these extracts on cell viability and intracellular ROS production was assayed using the U373-MG human astrocytoma cell line in a H2O2-induced oxidative stress model. Results showed that the major type of polyphenols found were benzoic acids, cinnamic acids, flavones and flavanones. The total phenolic content was 37.7 mg gallic acid/g sample and the ORAC value was 1.355 .mol TE/mg sample. The data obtained in cellular assays demonstrated that these ethanolic extracts protected H2O2-induced astrocyte damage by increasing cell viability and inhibiting production of intracellular ROS. These results suggest that the investigated extracts obtained from the aerial parts of M longifolia have antioxidant potential related to their phenol content which have important beneficial health effects, especially in those disease associated with ROS.
authors.forename: Tarek F
authors.initials: TF
authors.lastname: Eissa
authors.forename: Elena
authors.initials: E
authors.lastname: González-Burgos
authors.forename: M Emilia
authors.initials: ME
authors.lastname: Carretero
authors.forename: M Pilar
authors.initials: MP
authors.lastname: Gómez-Serranillos
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000431:Ethanol / Q000737:chemistry*; D005707:Gallic Acid / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D027541:Mentha / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010636:Phenols; D017382:Reactive Oxygen Species; D000431:Ethanol; D005707:Gallic Acid; D006861:Hydrogen Peroxide