title: [Build of focal cerebral ischemia model in different varieties of mice with modification monofilament].
abstract: OBJECTIVE
  To establish a general method of focal cerebral ischemia model in different varieties of mice.
  
  
  METHODS
  Each group of healthy adult KM and C57BL/6 mice were randomly divided into control group (n = 10) and MCAO group (n = 10). The mice in MCAO group were applied in the preparation of the MCAO model by intraluminal occlusion using monofilament. Twenty-four hours after operation,the neurologic function was evaluated,middle cerebral artery blood flow was monitored and the infarction volume was calculated by TTC staining, to evaluate the reliability of the model.
  
  
  RESULTS
  In the MCAO group, the base value of the cerebral blood flow down of KM and C57BL/6 mice respectively was (81.65 ± 4.59)%, (83.68 ± 6.25)%. The neurological deficit score respectively was (2.30 ± 0.82), (2.50 ± 0.80). TTC staining can clearly show the infarction area, and relatively stable, 24 hours of the survival rate of KM and C57BL/6 mice were 100% and 80% respectively.
  
  
  CONCLUSIONS
  The key link is the optimization and improvement of monofilament, temperature, anesthesia and so on. The modified intraluminal occlusion of MCAO using monofilament is a kind of reliable and simple method to establish experimental cerebral ischemia model in mice.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Jia
authors.forename: Zuo-Rong
authors.initials: ZR
authors.lastname: Shi
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Yang
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D001921:Brain / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D002545:Brain Ischemia / Q000150:complications / Q000503:physiopathology*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D020244:Infarction, Middle Cerebral Artery / Q000150:complications / Q000503:physiopathology*; D008297:Male; D008810:Mice, Inbred C57BL; D020768:Middle Cerebral Artery / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D009422:Nervous System Diseases / Q000209:etiology / Q000503:physiopathology; D013045:Species Specificity