title: Colorimetric detection of melamine based on methanobactin-mediated synthesis of gold nanoparticles.
abstract: A simple and rapid field-portable colorimetric method for the detection of melamine in liquid milk was reported. Methanobactin (Mb) could reduce Au (III) to Au (0) and mediate the synthesis of gold nanoparticles (Au-NPs). Upon the addition of melamine, melamine interacted with oxazolone ring of Mb, which interrupted the formation of Au-NPs. Melamine could also stimulate the aggregation of formed Au-NPs. In this paper, these characteristics have been used to detect melamine in liquid milk by naked eyes observation with a detection limit of 5.56 × 10(-6)M (0.7 mg/kg). Further, the plasmon absorbance of the formed Au-NPs allowed the quantitative detection of melamine by UV-vis spectrometer. A linear correlation was existed between the absorbance and the melamine concentration ranging from 3.90 × 10(-7)M to 3.97 × 10(-6)M with a correlation coefficient of 0.9685. The detection limit (3σ) obtained by UV-vis spectrum was as low as 2.38 × 10(-7)M (i.e., 0.03 mg/kg).
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China; State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China. Electronic address: Xinjiayingvip@163.com.
authors.forename: Jia-ying
authors.initials: JY
authors.lastname: Xin
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Lan-xuan
authors.initials: LX
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Chen
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lin
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Fan
authors.affiliation: Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Chun-gu
authors.initials: CG
authors.lastname: Xia
mesh_terms: D000818:Animals; D003124:Colorimetry / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D014227:Triazines / Q000737:chemistry*
chemical_list: D007093:Imidazoles; D009842:Oligopeptides; D014227:Triazines; C491147:methanobactin; D006046:Gold; C011907:melamine
keywords: Colorimetric detection; Gold nanoparticles; Liquid milk; Melamine; Methanobactin