title: Protective role of dietary-supplemented selenium and vitamin E in heat-induced apoptosis and oxidative stress in mice testes.
abstract: This study evaluated the role of selenium (0.5 ppm selenium/kg diet) and vitamin E (200 mg alpha-tocopherol/kg diet) on spermatogenesis after scrotal hyperthermia (42 °C, 30 min) in six different groups of male Balb/c mice; Control, Heat shock, Selenium, Selenium+heat shock, Vitamin E and Vitamin E+heat shock. Markers of the stress responses, hypoxia and oxidative stress, were evaluated in testis after the hyperthermic shock. Hyperthermia caused an elevated mRNA expression of hypoxia-inducible factor-1 alpha, haem oxygenase-1 (HMOX-1) and also glutathione peroxidase activity and reactive oxygen species (ROS). Apoptosis was evaluated by TUNEL assay and further by mRNA expression of Bcl-2, caspase 3, 8, 9, bid and AKT. TUNEL assay showed significant increase in apoptotic index of spermatogenic cells, whereas decrease in mRNA expression of Bcl-2, AKT and increase in caspase 3, 8, 9 and Bid in heat-shock group were observed. A significant decrease in sperm motility was also seen in heat-shock group in comparison with control group. These observations clearly indicate the development of oxidative stress and apoptosis after hyperthermia. Further analysis in Selenium+heat shock and Vitamin E+heat shock groups showed protective behaviour as compared to effects in heat-shock group which could be of therapeutic interest in future studies.
authors.affiliation: Department of Biophysics, Punjab University, Chandigarh, India.
authors.forename: S
authors.initials: S
authors.lastname: Kaur
authors.affiliation: Department of Biophysics, Punjab University, Chandigarh, India.
authors.forename: M P
authors.initials: MP
authors.lastname: Bansal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000032:analysis; D006358:Hot Temperature / Q000009:adverse effects; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D018384:Oxidative Stress / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000032:analysis; D012643:Selenium / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D008565:Membrane Proteins; D017382:Reactive Oxygen Species; D014810:Vitamin E; D005979:Glutathione Peroxidase; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D012643:Selenium
keywords: AKT; Bcl-2; Bid; antioxidants; apoptosis; caspases; selenium; spermatogenesis; vitamin E