title: Multiplex detection of enzymatic activity with responsive lanthanide-based luminescent probes.
abstract: Multiplex analyte detection in complex dynamic systems is desirable for the investigation of cellular communication networks as well as in medical diagnostics. A family of lanthanide-based responsive luminescent probes for multiplex detection is reported. The high modularity of the probe design enabled the rapid assembly of both green and red emitters for a large variety of analytes by the simple exchange of the lanthanide or an analyte-cleavable caging group, respectively. The real-time three-color detection of up to three analytes was demonstrated, thus setting the stage for the non-invasive investigation of interconnected biological processes.
authors.affiliation: Department of Chemistry-BMC, Uppsala University, Box 576, Uppsala, 75123 (Sweden).
authors.forename: Elias
authors.initials: E
authors.lastname: Pershagen
authors.forename: K Eszter
authors.initials: KE
authors.lastname: Borbas
mesh_terms: D004798:Enzymes / Q000378:metabolism*; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D049449:Luminescence; D015335:Molecular Probes
chemical_list: D004798:Enzymes; D028581:Lanthanoid Series Elements; D015335:Molecular Probes
keywords: enzymes; fluorescent probes; lanthanides; luminescence; reactive oxygen species