title: In vitro oxidation of fibrinogen promotes functional alterations and formation of advanced oxidation protein products, an inflammation mediator.
abstract: Fibrinogen (FB) is a soluble blood plasma protein and is a key molecule involved in coagulation. Oxidative modification of proteins, such as the formation of advanced oxidation protein products (AOPP), a heterogeneous family of protein compounds structurally modified and derived from oxidative stress, may be associated with the pathophysiology of a number of chronic inflammatory diseases. Therefore, the aim of this study was to determine whether the formation of this mediator of inflammation occurs from FB and whether its generation is associated with structural changes. Results of the present study suggest that the oxidation of FB may provoke the formation of AOPP, which in turn, may promote functional alterations in FB, thus causing changes in its structural domains and increasing its procoagulant activity.
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Center of Health Sciences, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Vanessa Dorneles
authors.initials: VD
authors.lastname: Torbitz
authors.forename: Guilherme Vargas
authors.initials: GV
authors.lastname: Bochi
authors.forename: José Antônio Mainardi
authors.initials: JA
authors.lastname: de Carvalho
authors.forename: Rodrigo
authors.initials: R
authors.lastname: de Almeida Vaucher
authors.forename: José Edson Paz
authors.initials: JE
authors.lastname: da Silva
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000378:metabolism*; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000494:pharmacology*; D007249:Inflammation / Q000276:immunology*; D018836:Inflammation Mediators / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D062107:Advanced Oxidation Protein Products; D018836:Inflammation Mediators; D006997:Hypochlorous Acid; D005340:Fibrinogen