title: Isolation and characterization of a gamma-aminobutyric acid producing strain Lactobacillus buchneri WPZ001 that could efficiently utilize xylose and corncob hydrolysate.
abstract: Lactobacillus buchneri strain WPZ001 that could efficiently produce gamma-aminobutyric acid was isolated from Chinese fermented sausages. Optimal cultivation conditions for gamma-aminobutyric acid production in L. buchneri WPZ001 were determined, and xylose was found to be the best carbon source. Using xylose as the sole carbon source, 70 g/L gamma-aminobutyric acid was produced by flask fermentation of L. buchneri WPZ001 for 48 h, and the harvested cells could continue to convert monosodium glutamate to gamma-aminobutyric acid in buffer and produce 59 g gamma-aminobutyric acid after eight runs of biotransformation; the total yield of gamma-aminobutyric acid reached 129 g/L. This combination strategy also worked well when the low-cost corncob hydrolysate was used as the sole carbon source, and the yield of gamma-aminobutyric acid reached 117 g/L. The results indicate that L. buchneri WPZ001 has great potential for industrial production of gamma-aminobutyric acid.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Zhao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Hu
authors.forename: Lu
authors.initials: L
authors.lastname: Pan
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Wang
mesh_terms: D002244:Carbon / Q000378:metabolism; D004275:DNA, Ribosomal; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D012970:Sodium Glutamate / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*; D003313:Zea mays; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D005680:gamma-Aminobutyric Acid; D002244:Carbon; D014994:Xylose; D009584:Nitrogen; D012970:Sodium Glutamate