title: Transcriptome characterization of the ascidian Pyura chilensis using 454-pyrosequencing data from two distant localities on the southeast Pacific.
abstract: This study describes the results from transcriptomes sequenced by 454-pyrosequencing from two populations separated by 10° of latitude of the endemic tunicate Pyura chilensis. Most transcripts were assembled in 43,972 contigs with an average length of 842 nucleotides. De novo assembly revealed that less than 30% of the contigs were annotated to Gene Ontology terms. A total of 71,662 single nucleotide polymorphisms (SNPs) were detected in 14,712 contigs. In silico differential expression of contigs annotated for SNPs revealed several genes differentially expressed in individuals collected from both populations. The present genomic resource will provide the basis to perform functional genomics on the species via the localization of genomic markers that can aid in determining levels of local adaptation, overall genetic structure and the genetic assessment of restocking programs for this species.
authors.affiliation: Laboratorio de Diversidad Molecular, Departamento de Biología Marina, Facultad de Ciencias del Mar, Universidad Católica del Norte, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile; Centro de Estudios Avanzados en Zonas Áridas (CEAZA), Coquimbo, Chile. Electronic address: phaye@ucn.cl.
authors.forename: Pilar A
authors.initials: PA
authors.lastname: Haye
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, University of Concepción, Concepción, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
mesh_terms: D000818:Animals; D012313:RNA / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D014561:Urochordata / Q000235:genetics*
chemical_list: D012313:RNA
keywords: Pyrosequencing; Pyura chilensis; RNA-Seq; SNP; Transcriptome; Tunicates