title: Electrochemiluminescent immune-modified electrodes based on Ag2Se@CdSe nanoneedles loaded with polypyrrole intercalated graphene for detection of CA72-4.
abstract: This work described a new electrochemiluminescence (ECL) immunosensor based on polypyrrole intercalated graphene and Ag2Se@CdSe nanoneedles. The novel nanomaterial Ag2Se@CdSe, with needle-like morphology, was synthesized for the first time. The prepared Ag2Se@CdSe nanoneedles exhibited good luminous performance in the presence of K2S2O8. Polypyrrole intercalated amination graphene with high specific binding sites and excellent electrochemical performance was used as the platform for the sensor. The developed ECL immunosensor was used for the detection of CA72-4 with good linear relation in the range from 10(-4) to 20 U/mL and low detection limit of 2.1 × 10(-5) U/mL (S/N = 3). The developed ECL immunosensor with high sensitivity and spectral selectivity can be used for detection of real samples. Ag2Se@CdSe nanoneedles could be promising candidate emitter for ECL biosensors in the future.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan , Jinan 250022, People's Republic of China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Lv
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000737:chemistry; D016014:Linear Models; D049449:Luminescence; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D049329:Nanostructures / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D018036:Selenium Compounds / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D007364:Intercalating Agents; D011108:Polymers; D011758:Pyrroles; D018036:Selenium Compounds; C067635:polypyrrole; D012834:Silver; D006108:Graphite; C058667:cadmium selenide
keywords: Ag2Se@CdSe nanoneedles; electrochemiluminescence; modified electrodes; polypyrrole intercalated graphene