title: An enzyme-linked immuno-mass spectrometric assay with the substrate adenosine monophosphate.
abstract: An enzyme-linked immuno-mass spectrometric assay (ELIMSA) with the specific detection probe streptavidin conjugated to alkaline phosphatase catalyzed the production of adenosine from the substrate adenosine monophosphate (AMP) for sensitive quantification of prostate-specific antigen (PSA) by mass spectrometry. Adenosine ionized efficiently and was measured to the femtomole range by dilution and direct analysis with micro-liquid chromatography, electrospray ionization, and mass spectrometry (LC-ESI-MS). The LC-ESI-MS assay for adenosine production was shown to be linear and accurate using internal (13)C(15)N adenosine isotope dilution, internal (13)C(15)N adenosine one-point calibration, and external adenosine standard curves with close agreement. The detection limits of LC-ESI-MS for alkaline phosphatase-streptavidin (AP-SA, ∼190,000 Da) was tested by injecting 0.1 μl of a 1 pg/ml solution, i.e., 100 attograms or 526 yoctomole (5.26E-22) of the alkaline-phosphatase labeled probe on column (about 315 AP-SA molecules). The ELIMSA for PSA was linear and showed strong signals across the picogram per milliliter range and could robustly detect PSA from all of the prostatectomy patients and all of the female plasma samples that ranged as low as 70 pg/ml with strong signals well separated from the background and well within the limit of quantification of the AP-SA probe. The results of the ELIMSA assay for PSA are normal and homogenous when independently replicated with a fresh standard over multiple days, and intra and inter diem assay variation was less than 10 % of the mean. In a blind comparison, ELIMSA showed excellent agreement with, but was more sensitive than, the present gold standard commercial fluorescent ELISA, or ECL-based detection, of PSA from normal and prostatectomy samples, respectively.
authors.affiliation: Department of Chemistry and Biology, Ryerson University, 350 Victoria Street, Toronto, ON, M5B 2K3, Canada.
authors.forename: Angelique
authors.initials: A
authors.lastname: Florentinus-Mefailoski
authors.forename: Antonius
authors.initials: A
authors.lastname: Soosaipillai
authors.forename: Jaimie
authors.initials: J
authors.lastname: Dufresne
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Diamandis
authors.forename: John G
authors.initials: JG
authors.lastname: Marshall
mesh_terms: D000249:Adenosine Monophosphate / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007610:Kallikreins / Q000032:analysis* / Q000097:blood; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis* / Q000097:blood; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019809:Streptavidin / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D000249:Adenosine Monophosphate; D019809:Streptavidin; D000469:Alkaline Phosphatase; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen