title: Replication attempt: "Effect of BMAP-28 antimicrobial peptides on Leishmania major promastigote and amastigote growth: role of leishmanolysin in parasite survival".
abstract: This study describes an attempt to replicate experiments from the paper "Effect of BMAP-28 Antimicrobial Peptides on Leishmania major Promastigote and Amastigote Growth: Role of Leishmanolysin in Parasite Survival," which was submitted to the Reproducibility Initiative for independent validation. The cathelicidin bovine myeloid antimicrobial peptide 28 (BMAP-28) and its isomers were previously shown to have potent antiparasitic activity against Leishmania major. We tested the effectiveness of L-BMAP-28 and two of its isomers, the D-amino acid form (D-BMAP-28) and the retro-inverso form (RI-BMAP-28), in both unamidated and amidated forms, as anti-leishmanial agents against Leishmania major promastigotes in vitro. We observed that L-BMAP-28, as well as its D and RI isomers, demonstrate anti-leishmanial activity against L. major promastigotes in vitro. The inhibitory effect was lower than what was seen in the original study. At 2 µM of amidated peptides, the viability was 94%, 36%, and 66% with L-, D- and RI-peptides, versus 57%, 6%, and 18% in the original study.
authors.affiliation: The Reproducibility Initiative, c/o Science Exchange, Palo Alto, California, United States of America.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Iorns
authors.affiliation: The Reproducibility Initiative, c/o Science Exchange, Palo Alto, California, United States of America.
authors.forename: William
authors.initials: W
authors.lastname: Gunn
authors.affiliation: Anti-Infectives Screening Core Facility, Department of Microbiology, New York University School of Medicine, New York, New York, United States of America.
authors.forename: Jessey
authors.initials: J
authors.lastname: Erath
authors.affiliation: Anti-Infectives Screening Core Facility, Department of Microbiology, New York University School of Medicine, New York, New York, United States of America.
authors.forename: Ana
authors.initials: A
authors.lastname: Rodriguez
authors.affiliation: LifeTein LLC, Hillsborough, New Jersey, United States of America.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: The Reproducibility Initiative, c/o Science Exchange, Palo Alto, California, United States of America.
authors.forename: Michael
authors.initials: M
authors.lastname: Benzinou
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D006801:Humans; D018320:Leishmania major / Q000187:drug effects*; D008666:Metalloendopeptidases / Q000378:metabolism*; D050296:Microbial Viability; D011506:Proteins / Q000494:pharmacology*
chemical_list: D000981:Antiprotozoal Agents; D011506:Proteins; D008666:Metalloendopeptidases