title: Evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis.
abstract: Photodynamic therapy (PDT) has been proven to be effective in disinfecting root canals. The aim of this present study was to evaluate the effects of PDT on the viability of Enterococcus faecalis using methylene blue (MB) and malachite green (MG) as photosensitizers. Solutions containing E. faecalis (ATCC 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with MB and MG. Samples were individually irradiated by the diode laser at a distance of 1mm for 30, 60, or 120 seconds. Colonyforming units (CFU) were determined for each treatment. PDT for 60 and 120 seconds with MG reduced E. faecalis viability significantly. Similar results were obtained when MB was used as photosensitizer. PDT using MB and MG have antibacterial effect against E. faecalis, showing potential to be used as an adjunctive antimicrobial procedure in endodontic therapy.
authors.affiliation: Endodontics Department, Grande Rio University (UNIGRANRIO), Rio de Janeiro, Brazil.
authors.forename: Emmanuel J
authors.initials: EJ
authors.lastname: Silva
authors.affiliation: Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis, Brazil.
authors.forename: Wagner P
authors.initials: WP
authors.lastname: Coutinho-Filho
authors.affiliation: Endodontics Department, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: Aurimar O
authors.initials: AO
authors.lastname: Andrade
authors.affiliation: Endodontics Department, Campinas State University (UNICAMP), Piracicaba, Brazil.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Herrera
authors.affiliation: Endodontics Department, Grande Rio University (UNIGRANRIO), Rio de Janeiro, Brazil.
authors.forename: Tauby S
authors.initials: TS
authors.lastname: Coutinho-Filho
authors.affiliation: Endodontics Department, Rio de Janeiro State University, Rio de Janeiro, Brazil.
authors.forename: Renato L
authors.initials: RL
authors.lastname: Krebs
mesh_terms: D058491:Bacterial Load / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects*; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D008751:Methylene Blue / Q000627:therapeutic use; D050296:Microbial Viability / Q000187:drug effects; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D012388:Root Canal Irrigants / Q000627:therapeutic use; D012394:Rosaniline Dyes / Q000627:therapeutic use; D012973:Sodium Hypochlorite / Q000627:therapeutic use; D013997:Time Factors
chemical_list: D017319:Photosensitizing Agents; D012388:Root Canal Irrigants; D012394:Rosaniline Dyes; C005095:malachite green; D012973:Sodium Hypochlorite; D008751:Methylene Blue