title: Development and use of retinal pigmented epithelial cell line from zebrafish (Danio rerio) for evaluating the toxicity of ultraviolet-B.
abstract: Danio rerio retinal pigmented epithelial (DrRPE) cell line, derived from the RPE tissue, was established and characterized. The cells were able to grow at a wide range of temperatures from 25°C to 32°C in Leibovitz's L-15 medium. The DrRPE cell line consists of epithelial cells with a diameter of 15-19 μm. The cell line was characterized by mitochondrial 12S rRNA gene, immunocytochemical analysis, and karyotyping. DrRPE cells treated with 10 μM of all-trans-retinol for 24 h readily formed lipid droplets. DrRPE cells were irradiated with narrowband ultraviolet-B (UV-B) radiation at different time periods of 0, 10, 20, and 40 min. The cells were subsequently examined for changes in morphology, cell viability, phagocytotic activity, mitochondrial distribution, nuclei morphology, generation of reactive oxygen species, and expression of apoptotic-related genes p53 and Cas3 by quantitative polymerase chain reaction. The results demonstrate that UV-B radiation can cause a considerable decrease in DrRPE cell viability as well as in phagocytotic activity. In addition, the results demonstrate that UV-B radiation can induce the degradation of mitochondria and DNA in cultured DrRPE cells.
authors.affiliation: 1 OIE Reference Laboratory for WTD, PG and Research Department of Zoology, C. Abdul Hakeem College , Vellore, India .
authors.forename: Kalaiselvi S Nathiga
authors.initials: KS
authors.lastname: Nambi
authors.forename: Seepoo Abdul
authors.initials: SA
authors.lastname: Majeed
authors.forename: Gani
authors.initials: G
authors.lastname: Taju
authors.forename: Sridhar
authors.initials: S
authors.lastname: Sivasubbu
authors.forename: Nithiyanandam Sundar
authors.initials: NS
authors.lastname: Raj
authors.forename: Nithiyanandam
authors.initials: N
authors.lastname: Madan
authors.forename: Azeez Sait Sahul
authors.initials: AS
authors.lastname: Hameed
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured / Q000528:radiation effects; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000528:radiation effects; D018675:Toxicity Tests / Q000379:methods*; D014466:Ultraviolet Rays / Q000009:adverse effects*; D015027:Zebrafish