title: MicroRNA-543 acts as an oncogene by targeting PAQR3 in hepatocellular carcinoma.
abstract: MicroRNAs (miRNAs) are small, non-coding RNAs that can act as oncogenes or tumor suppressor genes in human cancer. Increasing evidences indicate that deregulation of miRNAs contributes to the hepatocarcinogenesis. In this study, we demonstrated that the levels of miR-543 were dramatically increased in clinical hepatocellular carcinoma (HCC) tissues and cell lines. Moreover, forced expression of miR-543 promoted the proliferative and invasive potential of HepG2. We also identified PAQR3 as a direct target gene for miR-543 using a fluorescent reporter assay and western blot. The levels of PAQR3 were dramatically decreased in clinical hepatocellular carcinoma (HCC) tissues and cell lines. The mRNA levels of PAQR3 were inversely correlated with the miR-543 expression level.Thus, our finding provides a new insight into the mechanism of hepatocarcinogenesis, indicating a therapeutic potential of miR-543 in the treatment of HCC.
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pathology, General Hospital of Jinan Military Command Jinan 250000, Shandong, China.
authors.forename: Luting
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Ophthalmology, The Fourth Hospital of Harbin, Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of General Surgery, Heilongjiang Province Hospital Harbin 150000, Heilongjiang, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of CT, Affiliated Hospital of Jining Medical University Jining 272029, Shandong, China.
authors.forename: Mujie
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Information Technology, Jining Medical University Jining 272029, Shandong, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Infectious Disease, The Fourth Hospital of Harbin Medical University Harbin 150001, Heilongjiang, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhu
keywords: PAQR3; hepatocellular carcinoma; miR-543; oncogene