title: [Risk factors for bacterial vaginosis - a 3-year study in four medical centers].
abstract: BACKGROUND
  Bacterial vaginosis (BV) is the most common infection of the lower genital tract among women of reproductive age. The purpose of this study was to determine the frequency of BV and the important etiological agent Gardnerella vaginalis in Bulgarian patients of different age groups, as well as, the risk factors forBV METHODS: One thousand five hundred and twenty-three (1523) women aged 16 to 45 years without previous antimicrobial therapy were included in this study. The methods used were: scoring of Gram staining of vaginal smear and polymerase chain reaction (PCR) assay for G. vaginalis.
  
  
  RESULTS
  Positive for BV according to the microscopic examination criteria were 47.80% from the 1523 tested women. In 88.87% from the positive samples G. vaginalis was detected using PCR, thus demonstrating high comparability of the both methods and the leading role of G. vaginalis. The most affected with BV is the age group of 21-25 years (33.21% of all subjects with BV). The most important detected risk factors were: smoking (more than 55% with BV), single marital status (only 15% with BV are married), more than I sexual partner (more than 36% had changed the sexual partner), early onset of sexual activity (75% of B V asocciated subjects started their sexual activity by the age 15-18 years).
  
  
  CONCLUSIONS
  The established early age for aquiring BV among Bulgarian women is very important and alarming sign. This is the first study on the etiological role of G. vaginalis and on the risk factors for BV in Bulgaria.
authors.forename: R
authors.initials: R
authors.lastname: Gergova
authors.forename: K
authors.initials: K
authors.lastname: Tosheva
authors.forename: M
authors.initials: M
authors.lastname: Shtereva
authors.forename: Tz
authors.initials: T
authors.lastname: Iordanova
authors.forename: V
authors.initials: V
authors.lastname: Konsulova
authors.forename: A
authors.initials: A
authors.lastname: Petrova-Jerova
authors.forename: I
authors.initials: I
authors.lastname: Mitov
authors.forename: T
authors.initials: T
authors.lastname: Ilieva
authors.forename: T
authors.initials: T
authors.lastname: Strateva
mesh_terms: D000293:Adolescent; D000328:Adult; D002031:Bulgaria; D005260:Female; D006194:Gardnerella vaginalis / Q000302:isolation & purification*; D005840:Gentian Violet; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D010619:Phenazines; D012307:Risk Factors; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000453:epidemiology* / Q000382:microbiology; D055815:Young Adult
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet