title: Gel-aided sample preparation (GASP)--a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells.
abstract: We describe a "gel-assisted" proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.
authors.affiliation: TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
authors.forename: Roman
authors.initials: R
authors.lastname: Fischer
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002457:Cell Extracts; D002478:Cells, Cultured; D006801:Humans; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000180:Acrylic Resins; D002457:Cell Extracts; D020543:Proteome; C016680:polyacrylamide gels
keywords: Bottom-up proteomics; Comparative proteomics; Gel-assisted sample preparation; Sample preparation; Technology