title: Expression of the MT1 melatonin receptor in ovarian cancer cells.
abstract: Ovarian cancer (OC) is the leading cause of death among women with genital tract disorders. Melatonin exhibits oncostatic properties which it may effect through binding to its membrane receptor, MT1. The aim of this study was to determine the expression of MT1 in OC cells and to correlate this with clinical and pathological data. Immunohistochemistry was performed on 84 cases of OC. Normal ovarian epithelial (IOSE 364) and OC (SK-OV-3, OVCAR-3) cell lines were used to examine the MT1 expression at protein level using the western blot and immunofluorescence technique. The expression of MT1 was observed as cytoplasmic-membrane (MT1(CM)) and membrane (MT1(M)) reactions. A positive correlation between MT1(CM) and MT1(M) was found in all the studied cases. There were no significant differences between the expression of MT1(CM), MT1(M), and histological type, staging, grading, presence of residual disease, or overall survival time. Immunofluorescence showed both MT1(M) and MT1(CM) expression in all the tested cell lines. Western blot illustrated the highest protein level of MT1 in IOSE 364 and the lowest in the OVCAR-3. The results indicate the limited prognostic significance of MT1 in OC cells.
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. karolina.jablonska@umed.wroc.pl.
authors.forename: Karolina
authors.initials: K
authors.lastname: Jablonska
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. bartosz.pula@gmail.com.
authors.forename: Bartosz
authors.initials: B
authors.lastname: Pula
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. agatazemla@op.pl.
authors.forename: Agata
authors.initials: A
authors.lastname: Zemla
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. ch.kobierzycki@gmail.com.
authors.forename: Christopher
authors.initials: C
authors.lastname: Kobierzycki
authors.affiliation: Department of Gynecology, University of Medical Sciences, Poznań 60-535, Poland. witold.kedzia@poczta.fm.
authors.forename: Witold
authors.initials: W
authors.lastname: Kedzia
authors.affiliation: Department of Gynecological Oncology, University of Medical Sciences, Poznań 60-535, Poland. ewamarkwitz@poczta.fm.
authors.forename: Ewa
authors.initials: E
authors.lastname: Nowak-Markwitz
authors.affiliation: Department of Gynecological Oncology, University of Medical Sciences, Poznań 60-535, Poland. m.spaczynski@op.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Spaczynski
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. maciej.zabel@umed.wroc.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. marzenna.podhorska-okolow@umed.wroc.pl.
authors.forename: Marzenna
authors.initials: M
authors.lastname: Podhorska-Okolow
authors.affiliation: Department of Histology and Embryology, Wrocław Medical University, Wrocław 50-368, Poland. piotr.dziegiel@umed.wroc.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D044122:Receptor, Melatonin, MT1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D044122:Receptor, Melatonin, MT1