title: Isolation and characterization of a hyperbranched proteoglycan from Ganoderma lucidum for anti-diabetes.
abstract: Presently, an efficient protein tyrosine phosphatase 1B (PTP1B) inhibitor, named FYGL-n, was isolated from Ganoderma Lucidum and characterized for its structure and bioactivity. Structure and chain conformation of FYGL-n based on both chemical and spectroscopic analysis showed that FYGL-n was a hyperbranched heteropolysaccharide bonded with protein via both serine and threonine residues by O-type glycoside, and showed a sphere observed by AFM. Specifically, monosaccharide composition indicated that FYGL-n consisted of D-arabinose, D-galactose, L-rhamnose and D-glucose in a mole ratio of 0.08:0.21:0.24:0.47, with a molecular mass of 72.9 kDa. The analysis of amino acids in FYGL-n indicated that there were 16 common amino acids, among which aspartic acid, glycine, serine, alanine, glutamic acid and threonine were the dominant components. Also it was demonstrated that FYGL-n could inhibit the PTP1B activity on a competitive mechanism in vitro.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Deng
authors.initials: D
authors.lastname: Pan
authors.affiliation: Shanghai Key Laboratory of Magnetic Resonance, Department of Physics, East China Normal University, Shanghai 200062, PR China.
authors.forename: Linqiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China.
authors.forename: Congheng
authors.initials: C
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Magnetic Resonance, Department of Physics, East China Normal University, Shanghai 200062, PR China. Electronic address: bwhu@phy.ecnu.edu.cn.
authors.forename: Bingwen
authors.initials: B
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China. Electronic address: pingzhou@fudan.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
mesh_terms: D002240:Carbohydrate Sequence; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D008969:Molecular Sequence Data; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D011509:Proteoglycans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D020940:Reishi / Q000737:chemistry*
chemical_list: D004791:Enzyme Inhibitors; D007004:Hypoglycemic Agents; D011509:Proteoglycans; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1
keywords: Ganoderma Lucidum; Hyperbranched; Polysaccharide; Proteoglycan; Structure characterization