title: Residues investigation of fluoroquinolones and sulphonamides and their metabolites in bovine milk by quantification and confirmation using ultra-performance liquid chromatography-tandem mass spectrometry.
abstract: A novel UPLC-MS/MS method for quantification and confirmation of eight fluoroquinolones, five sulphonamides (SAs) and four acetyled metabolites in milk is developed. Their main fragmentation pathways were presented. The limits of quantification were in the range of 0.01-0.29 μg kg(-1) for FQs and 0.13-1.68 μg kg(-1) for SAs. Mean recoveries ranging from 61% to 115% were achieved at spiked level of 0.5-100 μg kg(-1). The intra- and inter-day precisions were in the range of 6.3-10.7% and 9.0-13.4%, respectively. This novel approach has high speed and sensitivity, and can ensure the multiclass and multiresidue analysis of bovine milk at low μg kg(-1) level. The content of each analyte residue is much lower than the maximum residue limits. Some residues in milk were confirmed in according to the Commission Decision 657/2002/EC.
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China; Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan 750021, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Meng
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Shi
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Shuxuan
authors.initials: S
authors.lastname: Liang
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Dong
authors.affiliation: Hebei Institute of Food Quality Supervision Inspection and Research, Shijiazhuang 050051, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. Electronic address: hanwen@hbu.edu.cn.
authors.forename: Hanwen
authors.initials: H
authors.lastname: Sun
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D013449:Sulfonamides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D024841:Fluoroquinolones; D013449:Sulfonamides
keywords: Bovine milk; Fluoroquinolones; Mass spectrometry; Metabolites; Multiresidue analysis; Sulphonamides; Ultra-high performance liquid chromatography