title: The Metabolism of Polysaccharide from Atractylodes macrocephala Koidz and Its Effect on Intestinal Microflora.
abstract: An active polysaccharide from the rhizome of Atractylodes macrocephala Koidz (PAM) was identified to improve and adjust disordered intestinal flora. High-performance gel permeation chromatography (HPGPC) and gas chromatography-mass spectrometry (GC-MS) were employed to identify the components of PAM as rhamnose, glucose, mannose, xylose, and galactose at a ratio of 0.03 : 0.25 : 0.15 : 0.41 : 0.15. PAM metabolized in gastrointestinal tract when incubated with artificial gastric and intestinal juices. Anaerobic incubation of PAM on intestinal flora confirmed that PAM promoted the ability of intestinal bacteria to digest reducing sugar. Based on the Shannon index and similarity coefficient index of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) fingerprinting of the total intestinal bacteria DNA, we concluded that PAM can significantly improve disordered intestinal flora and may be used as an oral adjuvant to regulate intestinal flora.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhou
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Mengyue
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Ying
authors.identifier: 0000-0003-2419-0702
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li