title: The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein.
abstract: Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein-oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.
authors.affiliation: Department of Chemical, Paper and Biomedical Engineering, Miami University, 650 E High St, Oxford, OH 45056, USA. berberj@miamioh.edu.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Falatach
authors.forename: Cameron
authors.initials: C
authors.lastname: McGlone
authors.forename: M Sameer
authors.initials: MS
authors.lastname: Al-Abdul-Wahid
authors.forename: Saadyah
authors.initials: S
authors.lastname: Averick
authors.forename: Richard C
authors.initials: RC
authors.lastname: Page
authors.forename: Jason A
authors.initials: JA
authors.lastname: Berberich
authors.forename: Dominik
authors.initials: D
authors.lastname: Konkolewicz
mesh_terms: D004789:Enzyme Activation; D057927:Hydrophobic and Hydrophilic Interactions; D008837:Micrococcus / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D009113:Muramidase / Q000737:chemistry* / Q000378:metabolism*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism
chemical_list: D011108:Polymers; D009113:Muramidase