title: Differential responses of Africanized and European honey bees (Apis mellifera) to viral replication following mechanical transmission or Varroa destructor parasitism.
abstract: For the first time, adults and brood of Africanized and European honey bees (Apis mellifera) were compared for relative virus levels over 48 h following Varroa destructor parasitism or injection of V. destructor homogenate. Rates of increase of deformed wing virus (DWV) for Africanized versus European bees were temporarily lowered for 12h with parasitism and sustainably lowered over the entire experiment (48 h) with homogenate injection in adults. The rates were also temporarily lowered for 24h with parasitism but were not affected by homogenate injection in brood. Rates of increase of black queen cell virus (BQCV) for Africanized versus European bees were similar with parasitism but sustainably lowered over the entire experiment with homogenate injection in adults and were similar for parasitism and homogenate injection in brood. Analyses of sac brood bee virus and Israeli acute paralysis virus were limited as detection did not occur after both homogenate injection and parasitism treatment, or levels were not significantly higher than those following control buffer injection. Lower rates of replication of DWV and BQCV in Africanized bees shows that they may have greater viral resistance, at least early after treatment.
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Mollah Md
authors.initials: MM
authors.lastname: Hamiduzzaman
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address: eguzman@uoguelph.ca.
authors.forename: Ernesto
authors.initials: E
authors.lastname: Guzman-Novoa
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Goodwin
authors.affiliation: Departamento de Medicina y Zootecnia en Abejas, FMVZ, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Mexico, D.F. 04960, Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Reyes-Quintana
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Gun
authors.initials: G
authors.lastname: Koleoglu
authors.affiliation: Departamento de Medicina y Zootecnia en Abejas, FMVZ, Universidad Nacional Autonoma de Mexico, Ciudad Universitaria, Mexico, D.F. 04960, Mexico.
authors.forename: Adriana
authors.initials: A
authors.lastname: Correa-Benítez
authors.affiliation: Department of Mathematics and Statistics, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Petukhova
mesh_terms: D000818:Animals; D001179:Arthropod Vectors / Q000502:physiology / Q000821:virology*; D001516:Bees / Q000469:parasitology / Q000821:virology*; D056629:Dicistroviridae / Q000472:pathogenicity / Q000502:physiology; D060467:Disease Resistance; D005247:Feeding Behavior; D006790:Host-Parasite Interactions; D010849:Picornaviridae / Q000472:pathogenicity / Q000502:physiology; D057077:Varroidae / Q000502:physiology / Q000821:virology*; D014779:Virus Replication
keywords: Africanized bees; European bees; Homogenate; Honey bee viruses; Injection; Varroa destructor