title: Puerarin attenuates airway inflammation by regulation of eotaxin-3.
abstract: Puerarin is an isoflavonoid isolated from the root of the plant Pueraria lobata and has been used as a prescribed drug in China for the treatment of many diseases in the clinical practice. The present study aimed to determine the protective effects and the underlying mechanisms of puerarin on ovalbumin (OVA)-induced allergic inflammation in a mouse model of allergic asthma. Asthma mice model was established by ovalbumin. A total of 50 mice were randomly assigned to five experimental groups: control, model, dexamethasone (2 mg/kg), and puerarin (10 mg/kg, 20 mg/kg). Airway resistance (Raw) was measured by the forced oscillation technique, differential cell count in BAL fluid (BALF) was measured by Wright-Giemsa staining, histological assessment was measured by hematoxylin and eosin (HE) staining, BALF levels of Th1/Th2 cytokines were measured by enzyme-linked immunosorbent assay, eotaxin-3 was evaluated by western blotting. Our study demonstrated that, compared with model group, puerarin inhibited OVA-induced increases in Raw and eosinophil count; interleukin (IL)-4, IL-5, IL-13 levels were recovered in bronchoalveolar lavage fluid compared; increased IFN-γ level in bronchoalveolar lavage fluid; histological studies demonstrated that puerarin substantially inhibited OVA-induced eosinophilia in lung tissue compared with model group. Western blotting studies demonstrated that puerarin substantially inhibited eotaxin-3 compared with model group. Our findings support puerarin can prevent some signs of allergic asthma in the mouse model.
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, China; Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, China.
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, China; Department of Pharmacology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: 705018816@126.com.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Ma
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: wangshuminch@126.com.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D000075906:Chemokine CCL26; D019742:Chemokines, CC / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D004802:Eosinophilia / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007529:Isoflavones / Q000494:pharmacology*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D008517:Phytotherapy / Q000379:methods; D029908:Pueraria / Q000737:chemistry; D011897:Random Allocation; D012137:Respiratory System / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018417:Th1 Cells / Q000378:metabolism; D018418:Th2 Cells / Q000378:metabolism
chemical_list: C000603142:CCL26 protein, mouse; D000075906:Chemokine CCL26; D019742:Chemokines, CC; D016207:Cytokines; D007529:Isoflavones; D010047:Ovalbumin; C033607:puerarin
keywords: Allergic airway inflammation; Eotaxin-3; Puerarin