title: Overexpression of malic enzyme in the larval stage extends Drosophila lifespan.
abstract: Metabolic modifications during the developmental period can extend longevity. We found that malic enzyme (Men) overexpression during the larval period lengthened the lifespan of Drosophila. Men overexpression by S106-GeneSwitch-Gal4 driver increased pyruvate content and NADPH/NADP(+) ratio but reduced triglyceride, glycogen, and ATP levels in the larvae. ROS levels increased unexpectedly in Men-overexpressing larvae. Interestingly, adults exposed to larval Men-overexpression maintained ROS tolerance with enhanced expression levels of glutathione-S-transferase D2 and thioredoxin-2. Our results suggest that metabolic changes mediated by Men during development might be related to the control of ROS tolerance and the longevity of Drosophila.
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Gye-Hyeong
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Gun-Ho
authors.initials: GH
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Youn-Ho
authors.initials: YH
authors.lastname: Cho
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Young-Nam
authors.initials: YN
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Yeogil
authors.initials: Y
authors.lastname: Jang
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea.
authors.forename: Donggi
authors.initials: D
authors.lastname: Paik
authors.affiliation: Department of Physiology, College of Medicine, Korea University, 73 Inchon-ro, Seongbuk-gu, Seoul 136-705, Republic of Korea. Electronic address: parkjj@korea.ac.kr.
authors.forename: Joong-Jean
authors.initials: JJ
authors.lastname: Park
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D029721:Drosophila Proteins / Q000096:biosynthesis* / Q000235:genetics; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005982:Glutathione Transferase / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007814:Larva / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D008136:Longevity / Q000235:genetics / Q000502:physiology*; D008291:Malate Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D009249:NADP / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D017382:Reactive Oxygen Species; D014280:Triglycerides; D013879:Thioredoxins; D009249:NADP; D019289:Pyruvic Acid; D000255:Adenosine Triphosphate; D006003:Glycogen; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); C031917:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); D005982:Glutathione Transferase
keywords: Drosophila; Longevity; Malic enzyme; Pyruvate; ROS