title: Adding MgO nanoparticles to hydroxyapatite-PLLA nanocomposites for improved bone tissue engineering applications.
abstract: Magnesium plays an important role in the body, mediating cell-extracellular matrix interactions and bone apatite structure and density. This study investigated, for the first time, the effects of adding magnesium oxide (MgO) nanoparticles to poly (l-lactic acid) (PLLA) and to hydroxyapatite (HA) nanoparticle-PLLA composites for orthopedic tissue engineering applications. Results showed that MgO nanoparticles significantly enhanced osteoblast adhesion and proliferation on HA-PLLA nanocomposites while maintaining mechanical properties (Young's modulus ∼1,000 MPa) suitable for cancellous bone applications. Additionally, osteoblasts (or bone-forming cells) cultured in the supernatant of degrading nanocomposites showed improved proliferation in the presence of magnesium, indicating that the increased alkalinity of solutions containing MgO nanocomposites had no toxic effects towards cells. These results together indicated the promise of further studying MgO nanoparticles as additive materials to polymers to enhance the integration of implanted biomaterials with bone.
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Hickey
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
authors.forename: Batur
authors.initials: B
authors.lastname: Ercan
authors.affiliation: Department of Bioengineering, Northeastern University, Boston, MA 02115, USA.
authors.forename: Linlin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA; Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: th.webster@neu.edu.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Webster
mesh_terms: D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000494:pharmacology*; D008277:Magnesium Oxide / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D014961:X-Ray Diffraction
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; D008277:Magnesium Oxide; C033616:poly(lactide); D017886:Durapatite
keywords: Magnesium; Nanotechnology; Orthopedic; Osteoblasts