title: Toxicity Assessment of Cadinene Sesquiterpenes from Eupatorium adenophorum in Mice.
abstract: This study evaluated toxic efficacy of Eupatorium adenophorum extracts, against the Kunming mice. In acute study, we firstly tested median lethal dose (LD50) in mice of three cadinene sesquiterpenes 2-deoxo-2-(acetyloxy)-9-oxoageraphorone (DAOA), 9-oxo-agerophorone (OA) and 9-oxo-10,11-dehydro-agerophorone (ODA) from Eupatorium adenophorum (Ea). DAOA (215-4640 mg/kg BW, given orally) showed lowest LD50at 926 mg/kg BW for male mice in contrast with OA (1470 mg/kg BW) and ODA (1470 mg/kg BW). In sub-acute study, repeated doses (75-300 mg/kg BW, for 7 days) of DAOA/OA increased blood parameters, liver and spleen index in dose dependent relationship, along with decrease in thymus index. The blood biochemical and histopathological examination showed that DAOA/OA dose 300 mg/kg BW significantly causes pathological changes of hepatic lobules and hepatocytes, which are consistent with cholestasis and hepatic injury. 75 mg/kg dose of DAOA/OA was found to be approximately/totally safe over the span of 7 days treatment showing no change in all above described parameters. Cadinene sesquiterpenes guarantee low risk to environment as a type of low toxic botanical components, which may find potential application in biopesticides development field.
authors.affiliation: Department of Pesticides, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Ministry of Agriculture, and State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing, 100193, People's Republic of China.
authors.forename: Can-Bin
authors.initials: CB
authors.lastname: Ouyang
authors.forename: Xiao-Man
authors.initials: XM
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Hou-Yong
authors.initials: HY
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Wang
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Yan
authors.forename: Lian-Gang
authors.initials: LG
authors.lastname: Mao
authors.forename: Aocheng
authors.initials: A
authors.lastname: Cao
authors.forename: Mei-Xia
authors.initials: MX
authors.lastname: Guo