title: Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa.
abstract: The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group. Fresh spermatozoa displayed 45- and 35- kDa protein bands, while those of freeze-thawed andcapacitated spermatozoa displayed 45-, 35- and 28-kDa bands. In summary, these data indicate that proacrosin is activated, thus becoming α- and β-acrosins and a 28-kDa protein during capacitation and freeze-thawing.
authors.affiliation: Agricultural College of Yanbian University, Yanji, Jinlin, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cui
authors.affiliation: Agricultural College of Yanbian University, Yanji, Jinlin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Agricultural College of Yanbian University, Yanji, Jinlin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Agricultural College of Yanbian University, Yanji, Jinlin, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Huang
authors.affiliation: Agricultural College of Yanbian University, Yanji, Jinlin, China yijin@ybu.edu.cn.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000176:Acrosin / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D015925:Cryopreservation; D004792:Enzyme Precursors / Q000378:metabolism*; D005453:Fluorescence; D008297:Male; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000378:metabolism*; D013194:Staining and Labeling; D013552:Swine / Q000378:metabolism*
chemical_list: D004792:Enzyme Precursors; C022438:proacrosin; D000176:Acrosin