title: Occurrence of novel and rare subtype families of Cryptosporidium in bamboo rats (Rhizomys sinensis) in China.
abstract: This report is the first to describe Cryptosporidium infection in bamboo rats (Rhizomys sinensis). Ninety-two fresh fecal specimens were collected from a pet market in Ya'an City, China. One Cryptosporidium isolate from an asymptomatic host and two isolates from separate hosts with diarrhea were obtained by using Sheather's sucrose flotation technique and modified acid-fast staining. The Cryptosporidium spp. were genotyped by nested PCR and nucleotide sequencing of the small subunit rRNA (SSU rRNA), 70-kDa heat shock protein (HSP70), oocyst wall protein (COWP), and actin genes: isolates were identified as Cryptosporidium parvum with minor nucleotide differences at all four loci. Further subtyping was performed by PCR amplification and DNA sequence analysis of the 60-kDa glycoprotein (gp60) gene: two subtype families were detected, including a novel C. parvum subtype IIpA9 and a rare subtype IIoA13G1 (only reported in diarrheal patients of Sweden). Our results suggest that the bamboo rat is a reservoir host of C. parvum. Significantly, we discovered that the rare C. parvum subtype family IIo is also a zoonotic subtype and confirmed C. parvum subtype IIpA9 as a novel subtype family.
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Xuehan
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Jinjiang
authors.initials: J
authors.lastname: Shi
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Yingzhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China.
authors.forename: Baichun
authors.initials: B
authors.lastname: Qing
authors.affiliation: The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya(')an 625014, PR China. Electronic address: pgn.sicau@163.com.
authors.forename: Guangneng
authors.initials: G
authors.lastname: Peng
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D003457:Cryptosporidiosis / Q000453:epidemiology* / Q000469:parasitology; D003458:Cryptosporidium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016785:Cryptosporidium parvum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005838:Genotype; D058885:Multilocus Sequence Typing / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015800:Protozoan Proteins / Q000235:genetics; D051381:Rats; D012376:Rodent Diseases / Q000453:epidemiology* / Q000469:parasitology; D012377:Rodentia; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins
keywords: Bamboo rat; C. parvum; Cryptosporidium; SSU rRNA; Zoonotic subtype; gp60