title: Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners.
abstract: Cytosolic ascorbate peroxidase 1 (APX1) plays a crucial role in regulating the level of plant cellular reactive oxygen species and its thermolability is proposed to cause plant heat-susceptibility. Herein, several hyper-acidic fusion partners, such as the C-terminal peptide tails, were evaluated for their effects on the thermal stability and activity of APX1 from Jatropha curcas and Arabidopsis. The hyper-acidic fusion partners efficiently improved the thermostability and prevented thermal inactivation of APX1 in both plant species with an elevated heat tolerance of at least 2 °C. These hyper-acidified thermostable APX1 fusion variants are of considerable biotechnological potential and can provide a new route to enhance the heat tolerance of plant species especially of inherent thermo-sensitivity.
authors.affiliation: Key Laboratory of Biomass Energy and Environmental Biotechnology of Yunnan Province, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming, 650500, Yunnan, China, zrcxfx@sina.com.
authors.forename: Mengru
authors.initials: M
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Gong
authors.forename: Yumei
authors.initials: Y
authors.lastname: Yang
authors.forename: Xujuan
authors.initials: X
authors.lastname: Li
authors.forename: Haibo
authors.initials: H
authors.lastname: Wang
authors.forename: Zhurong
authors.initials: Z
authors.lastname: Zou
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000528:radiation effects; D060387:Ascorbate Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D031286:Jatropha / Q000201:enzymology* / Q000528:radiation effects; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D060387:Ascorbate Peroxidases