title: HSPD1 interacts with IRF3 to facilitate interferon-beta induction.
abstract: The production of IFN- I (IFN-α/β) is one of the earliest and most important host-protective responses. Interferon regulatory factor 3 (IRF3) is a critical transcriptional factor in the IFN-β signaling pathway. Although significant progress has been achieved in the regulation of IRF3, the process may be more complicated than previously considered. In the present study, heat shock protein 60 (HSP60, HSPD1) was identified as a novel IRF3-interacting protein. Overexpression of HSPD1 facilitated the phosphorylation and dimerization of IRF3 and enhanced IFN-β induction induced by SeV infection. In contrast, knockdown of endogenous HSPD1 significantly inhibited the signaling pathway. Furthermore, HSPD1 enhanced activation of the IFN-β promoter mediated by RIG-I, MDA-5, MAVS, TBK1 and IKKε but not IRF3/5D, a mock phosphorylated form of IRF3. The present study indicated that HSPD1 interacted with IRF3 and it contributed to the induction of IFN-β.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Pan
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Agriculture, Liaocheng University, Liaocheng, Shandong, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Pingan
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Xinlin
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D018834:Chaperonin 60 / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D050838:Interferon Regulatory Factor-3 / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D016899:Interferon-beta / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D060066:Protein Interaction Maps
chemical_list: D018834:Chaperonin 60; C060293:HSPD1 protein, human; C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; D024101:Mitochondrial Proteins; D016899:Interferon-beta