title: DNA binding and cytotoxicity activity of a chiral iron(III) triangle complex based on a natural rosin product.
abstract: The first chiral trinuclear iron(III) complex [Fe3(μ3-O)(L)6(CH3OH)2(CH3O)]⋅H2O (2) of a natural rosin product dehydroabietic acid (HL, 1) was synthesized and fully characterized. The interactions of 1 and 2 with salmon sperm DNA were investigated by viscosity measurements, UV, fluorescence and circular dichroism (CD) spectroscopic techniques. Absorption spectral (Kb=1.30×10(5)M(-1) (1), 1.40×10(5)M(-1)(2)), emission spectral (KSV=1.19×10(4)M(-1) (1), 1.79×10(4)M(-1) (2)) and viscosity measurements reveal that 1 and 2 interacted with DNA through intercalation and 2 exhibited a slight higher DNA binding ability. The Stern-Volmer quenching constant (KSV) and corresponding thermodynamic parameters (ΔG, ΔH and ΔS) of the binding processes were determined. The negative ΔH and ΔG values indicate that the binding reactions were exothermic and spontaneous. 1 and 2 were also screened for their cytotoxic ability and 1 demonstrated higher growth inhibition of the selected cancer cells at concentrations of 25μM and 50μM, this result was not identical with their DNA binding ability order. Thus, the involvement of Fe(III) centers had positive effect on DNA binding ability and negative effect on cytotoxicity.
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Coordination Chemistry Institute, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China. Electronic address: hgfbl@njfu.edu.cn.
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: Fei
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Wu-Shuang
authors.initials: WS
authors.lastname: Xu
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Wei-Lin
authors.initials: WL
authors.lastname: Gao
authors.affiliation: College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Coordination Chemistry Institute, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Science, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Long
authors.affiliation: Institute of Inorganic Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, D-76131 Karlsruhe, Germany.
authors.forename: Christopher E
authors.initials: CE
authors.lastname: Anson
authors.affiliation: Institute of Inorganic Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, D-76131 Karlsruhe, Germany.
authors.forename: Annie K
authors.initials: AK
authors.lastname: Powell
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002942:Circular Dichroism; D056831:Coordination Complexes / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008968:Molecular Conformation; D012116:Resins, Plant / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013816:Thermodynamics; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D005290:Ferric Compounds; D012116:Resins, Plant; C013893:rosin; D004247:DNA