title: Simple analytical strategy for MALDI-TOF-MS and nanoUPLC-MS/MS: quantitating curcumin in food condiments and dietary supplements and screening of acrylamide-induced ROS protein indicators reduced by curcumin.
abstract: Curcumin is the major active ingredient of turmeric and is widely used as a preservative, flavouring and colouring agent. Curcumin is a potent substance with several functions, including antioxidant, antitumor, anti-inflammatory, antimicrobial, antiparasitic, antimutagenic, chemopreventive and chemotherapeutic activities. Matrix-assisted laser desorption/ionisation coupled with time-of-flight mass spectrometry (MALDI-TOF-MS) has been used to analyse various molecules (including natural antioxidants). This study established an expeditious method that couples MALDI-TOF-MS with a simple dilution method to quantify curcumin in food condiments and dietary supplements. The linear range of curcumin detection ranged from 1 to 100 μg/mL. In further experiments, liver cells were treated with curcumin after exposure to acrylamide. Nano ultra performance liquid chromatographic system (nanoUPLC) coupled with tandem mass spectrometry (MS/MS) was used to evaluate the potential proteins and protein modifications induced by acrylamide. The results indicate that curcumin reduces the effects of reactive oxygen species induced by acrylamide.
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Yu-Shu
authors.initials: YS
authors.lastname: Huang
authors.affiliation: Department of Genome Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.
authors.forename: Tusty-Jiuan
authors.initials: TJ
authors.lastname: Hsieh
authors.affiliation: Department of Biochemistry, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Electronic address: cylu@kmu.edu.tw.
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Lu
mesh_terms: D020106:Acrylamide / Q000737:chemistry*; D000975:Antioxidants; D002853:Chromatography, Liquid; D003212:Condiments / Q000032:analysis*; D003474:Curcumin / Q000737:chemistry*; D019587:Dietary Supplements / Q000032:analysis*; D017382:Reactive Oxygen Species; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D020106:Acrylamide; D003474:Curcumin
keywords: Acrylamide; Curcumin; MALDI-TOF-MS; nanoUPLC–MS/MS