title: [The role of histone H3 acetylation on cleft palate in mice induced by 2, 3, 7, 8-tetrachlorodibenzopdioxin ].
abstract: OBJECTIVE
  To explore the role of histone H3 acetylation in cleft palate induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin (TCDD) in C57BL/6J mice, and its mechanism.
  
  
  METHODS
  On gestation day 10 (GD10), 36 pregnant mice were randomly divided into two groups as the treated group(n = 18) and the control group( n = 18). The mice in the treated group received intragastric administration with TCDD 28 μg/kg, while the mice in the control group received equivalent corn oil. The pregnant mice were sacrificed on GD13. 5, GD14. 5 and GD15. 5, collecting fetal palates to determine the activities of histone acetyltransferases (HATs) by Colorimetric and the expression level of acetylated histone H3 (Acetylated histone H3, Ac-H3) by Western-blot.
  
  
  RESULTS
  The activity of HATs was 0.409 7 ± 0.0147, 0.522 3 ± 0.017 1 and 0.643 5 ± 0.013 9 in control group on GD13.5, GD14.5 and GD15.5; 0.865 0 ± 0.0129, 0.719 1 ± 0.017 8 and 0.551 2 ± 0.016 8 in TCDD group. The activity of HATs in TCDD group was higher than that in control group on GD13. 5, GD14. 5, showing significantly difference between the two groups (t = - 56. 932, t = - 19. 516, P < 0.01); however, the activity of HATs in TCDD group was significantly lower than that in control group on GD15. 5 (t = 10. 382, P < 0.01). The expression level of Ac-H3 was 0.745 0 ± 0.113 5, 1.055 9 ± 0.249 4 and 1.795 5 ± 0.081 9 in control group on GD13. 5, GD14. 5 and GD15. 5; while 1.4490 ± 0. 1460, 1. 641 8 ± 0.099 7 and 1. 512 1 ± 0. 150 2 in TCDD group. The expression of Ac-H3 in TCDD group was higher than that in control group on GD13. 5, GD14. 5, showing significantly difference( t = -6. 593, -3. 779, P <0. 01, P <0.05) ; However, the expression of Ac-H3 in TCDD group was statistically lower than that in control group (t = 2. 870, P <0. 05).
  
  
  CONCLUSIONS
  The acetylation of histone H3 was involved in the cleft palate of C57BL/6J mice induced by TCDD, which may be one of the mechanisms in TCDD-induced cleft palate.
authors.forename: Liu
authors.initials: L
authors.lastname: Cuiping
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xingang
authors.forename: Fu
authors.initials: F
authors.lastname: Yuexian
authors.forename: Qiu
authors.initials: Q
authors.lastname: Lin
authors.forename: Tian
authors.initials: T
authors.lastname: Xiaofei
authors.forename: Liu
authors.initials: L
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Guanghui
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002972:Cleft Palate / Q000139:chemically induced / Q000378:metabolism*; D004147:Dioxins; D005260:Female; D005333:Fetus; D006657:Histones / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D000072317:Polychlorinated Dibenzodioxins; D011247:Pregnancy; D011897:Random Allocation; D013723:Teratogens
chemical_list: D004147:Dioxins; D006657:Histones; D000072317:Polychlorinated Dibenzodioxins; D013723:Teratogens; C038902:1,4-dioxin; D000123:Acetyltransferases