title: A fluorescence-quenching platform based on biomineralized hydroxyapatite from natural seashell and applied to cancer cell detection.
abstract: As a typical biomineral, hydroxyapatite (HAp) is widely applied in bone implants and other related fields. However, the inherent nature of HAp can potentially be altered through restricting its fabrication conditions. Here, HAp fabricated by a hydrothermal treatment of pieces of natural seashell is demonstrated to have the capability of fluorescence quenching. To the best of the author's knowledge, this is the first time that this new property of HAp has been reported. Consequently, we assembled a fluorescence-quenching platform based on the biomineralized HAp substrate following a hydrothermal treatment and associated with a DNA molecular beacon and applied to cancer cell detection by the transformation from "OFF state" (fluorescence quenching) to "ON state" (fluorescence recovery). Herein, we found that the outer surface of HAp material after hydrothermal biomineralization for 5 days has considerable capability for both fluorescence quenching and recovery. These results may also have implications in the further detection of various targets such as cancer cells with other special surface antigens, significant biological small molecules or disease related microRNA, just by changing the sequence of the nucleic acid beacon according to the corresponding aptamer.
authors.affiliation: 1] Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry &Chemical Engineering [2] National Engineering Research Center for Nanomedicine, College of Life Science &Technology, Huazhong University of Science &amp;Technology, 1037 Luoyu Road, Wuhan 430074, P.R.China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Nanomedicine, College of Life Science &Technology, Huazhong University of Science &amp;Technology, 1037 Luoyu Road, Wuhan 430074, P.R.China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Faculty of Science and Engineering, School of Chemistry and the Environment, Division of Chemistry and Environmental Science, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, Lancs, UK.
authors.forename: Craig E
authors.initials: CE
authors.lastname: Banks
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry &Chemical Engineering.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry &Chemical Engineering.
authors.forename: Mao
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory for Large-Format Battery Materials and System, Ministry of Education, School of Chemistry &Chemical Engineering.
authors.forename: Fan
authors.initials: F
authors.lastname: Xia
authors.affiliation: National Engineering Research Center for Nanomedicine, College of Life Science &Technology, Huazhong University of Science &amp;Technology, 1037 Luoyu Road, Wuhan 430074, P.R.China.
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Yang
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology
chemical_list: D017886:Durapatite