title: Marinobacter piscensis sp. nov., a moderately halophilic bacterium isolated from salty food in Tunisia.
abstract: An aerobic, Gram-negative, moderately halophilic bacterium, oxidase, and catalase positive-designated Abdou3(T), was isolated from salted traditional foods (Anchovies) in Tunisia. Cells were rod-shaped, non-spore-forming and motile. Growth occurred at 15-45 °C (optimum, 37 °C), pH 5.5-8.75 (optimum, 7.3), and in the presence of 1-15 % NaCl (optimum, 10 %). Strain Abdou3(T) used glucose, D-arabinose, and sucrose. Strain Abdou3(T) had Q9 as the major respiratory quinone and C18:1 ω9c and C16:0 as predominant fatty acids. The DNA G+C content was 55.2 mol%. Phylogenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence indicated that strain Abdou3(T) had as its closest relative Marinobacter maritimus (identity of 96 %). Based on phenotypic, phylogenetic, and taxonomic characteristics, strain Abdou3(T) is proposed as a novel species of the genus Marinobacter within the order Alteromonadales, for which the name M. piscensis sp. nov. is proposed. The type strain is Abdou3(T) (=DSM 26804(T)).
authors.affiliation: Laboratoire Microorganismes et Biomolécules Actives, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092, El Manar, Tunisia.
authors.forename: Abdeljabbar
authors.initials: A
authors.lastname: Hedi
authors.forename: Jean Luc
authors.initials: JL
authors.lastname: Cayol
authors.forename: Najla
authors.initials: N
authors.lastname: Sadfi
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D050260:Carbohydrate Metabolism; D002374:Catalase / Q000032:analysis; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D053520:Marinobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D014416:Tunisia
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D010088:Oxidoreductases; D002374:Catalase