title: The potential of SIRT6 and SIRT7 as circulating markers for head and neck squamous cell carcinoma.
abstract: BACKGROUND
  We have previously demonstrated altered SIRT gene family in head and neck squamous cell carcinoma (HNSCC). In the present study we aimed to investigate whether the SIRT gene family was also altered in peripheral blood (PB) of patients with HNSCC and the possibility to be used as circulating biomarkers.
  
  
  METHODS
  The expression profiles of the 7 SIRT genes of PB leukocytes from 34 patients with HNSCC before and after surgery and 31 healthy individuals were investigated.
  
  
  RESULTS
  In the cancer group, the expression level of SIRT1 was down-regulated (p<0.05); in contrast, SIRT6 and SIRT7 were significantly up-regulated (p<0.001). Patients with advanced-stage HNSCC had lower expression of SIRT1 and SIRT3. Recovery of SIRT6 and SIRT7 was observed in postoperative patients (p<0.005).
  
  
  CONCLUSIONS
  SIRT genes were altered in PB leukocytes of HNSCC patients and SIRT6 and SIRT7 are potential circulating prognostic markers for HNSCC.
authors.affiliation: Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Cheng-Tung
authors.initials: CT
authors.lastname: Lu
authors.affiliation: Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, R.O.C.
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Hsu
authors.affiliation: Department of Nursing, I-Shou University, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Pai-Mei
authors.initials: PM
authors.lastname: Lin
authors.affiliation: Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Chi-Chih
authors.initials: CC
authors.lastname: Lai
authors.affiliation: Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Hsin-Ching
authors.initials: HC
authors.lastname: Lin
authors.affiliation: Department of Otolaryngology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung City, Taiwan, R.O.C. Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, R.O.C.
authors.forename: Chao-Hui
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Division of Hematology-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C. Faculty of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Hsiao
authors.affiliation: Division of Hematology-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C. Faculty of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Yi-Chang
authors.initials: YC
authors.lastname: Liu
authors.affiliation: Division of Nephrolog, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C. Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung City, Taiwan, R.O.C.
authors.forename: Hugo You-Hsien
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Division of Hematology-Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, R.O.C. Faculty of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan, R.O.C. shlin@cc.kmu.edu.tw yangmy@mail.cgu.edu.tw.
authors.forename: Sheng-Fung
authors.initials: SF
authors.lastname: Lin
authors.affiliation: Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan, R.O.C. shlin@cc.kmu.edu.tw yangmy@mail.cgu.edu.tw.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000235:genetics / Q000601:surgery; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000097:blood* / Q000235:genetics / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D056564:Sirtuin 1 / Q000096:biosynthesis; D056566:Sirtuin 3 / Q000096:biosynthesis; D037761:Sirtuins / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics; D000077195:Squamous Cell Carcinoma of Head and Neck
chemical_list: D014408:Biomarkers, Tumor; C510661:SIRT7 protein, human; C447939:SIRT1 protein, human; C466093:SIRT3 protein, human; C516592:SIRT6 protein, human; D056564:Sirtuin 1; D056566:Sirtuin 3; D037761:Sirtuins
keywords: HNSCC; SIRT6; SIRT7; peripheral blood leukocytes; sirtuin genes