title: In vivo and in vitro observations of polyhydroxybutyrate granules formed by Dinoroseobacter sp. JL 1447.
abstract: Polyhydroxybutyrate (PHB) granules formed by a marine aerobic anoxygenic phototrophic bacterial strain Dinoroseobacter sp. JL 1447 were detected using transmission electron microscopy and atomic force microscopy. When Dinoroseobacter sp. JL 1447 was inoculated into a medium with glucose as the sole carbon source, the formation of PHB granules occurred and accumulated with incubation time, reaching their maximum in the stationary phase cultures. PHB granules, formed in the cytoplasm at the cell poles or future cell poles, were remobilized and used by the cells in late stationary complex cultures. When PHB granules formed, cell length elongated from 0.5 to 1.5 μm and spherical protrusions appeared on the cell surface. The French press method was used to break the cells and isolate the PHB granules. The freshly prepared and intact PHB granules were spherical with a soft, smooth outer envelope without visible substructures. Upon treating PHB granules with sodium dodecyl sulfate, the envelope was destroyed and nearly parted from the granules, and uniform, spherical structures with a central pore appeared on the granule surface.
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China; Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361102, China. Electronic address: Jiao@xmu.edu.cn.
authors.forename: Nianzhi
authors.initials: N
authors.lastname: Jiao
authors.affiliation: Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000378:metabolism; D018625:Microscopy, Atomic Force; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D041902:Rhodobacteraceae / Q000378:metabolism / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006885:Hydroxybutyrates; D011108:Polymers
keywords: Atomic force microscopy (AFM); Morphology; Polyhydroxybutyrate granules; Transmission electron microscopy (TEM)