title: Galactose grafting on poly(ε-caprolactone) substrates for tissue engineering: a preliminary study.
abstract: The grafting of galactose units onto poly(ε-caprolactone) (PCL) substrates by a wet chemistry two-step procedure is proposed. Even though a reduction of hardness from 0.58-0.31 GPa to 0.12-0.05 GPa is achieved, the chemical functionalization does not negatively affect the tensile modulus (332.2±31.3 MPa and 328.5±34.7 MPa for unmodified and surface-modified PCL, respectively) and strength (15.1±1.3 MPa and 14.8±1.5 MPa as assessed before and after the surface modification, respectively), as well as the mechanical behaviour evaluated through small punch test. XPS and enzyme-linked lectin assay (ELLA) demonstrate the presence, and also the correct exposition of the saccharidic epitope on PCL substrates. The introduction of carbohydrate moieties on the PCL surfaces clearly enhances the hydrophilicity of the substrate, as the water contact angle decreases from 82.1±5.8° to 62.1±4.2°. Furthermore, preliminary biological analysis shows human mesenchymal stem cell viability over time and an improvement of cell adhesion and spreading.
authors.affiliation: Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Russo
authors.affiliation: Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare-PAD. 20, 80125 Naples, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Russo
authors.affiliation: Department of Sciences, INSTM, CNISM and CISDiC, University Roma Tre, Via della Vasca Navale 79, 00146 Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Battocchio
authors.affiliation: Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Taraballi
authors.affiliation: Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare-PAD. 20, 80125 Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gloria
authors.affiliation: Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare-PAD. 20, 80125 Naples, Italy.
authors.forename: Ugo
authors.initials: U
authors.lastname: D'Amora
authors.affiliation: Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare-PAD. 20, 80125 Naples, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: De Santis
authors.affiliation: Department of Sciences, INSTM, CNISM and CISDiC, University Roma Tre, Via della Vasca Navale 79, 00146 Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Polzonetti
authors.affiliation: Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Nicotra
authors.affiliation: Institute of Polymers, Composites and Biomaterials-National Research Council of Italy, Viale J.F. Kennedy 54, Mostra d'Oltremare-PAD. 20, 80125 Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
authors.affiliation: Dept. of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy. Electronic address: laura.cipolla@unimib.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Cipolla
mesh_terms: D000588:Amines / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005690:Galactose / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D013718:Tensile Strength; D023822:Tissue Engineering
chemical_list: D000588:Amines; D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D005690:Galactose
keywords: Biological evaluation; Carbohydrates; Nanoindentation; Poly(ε-caprolactone); Small punch test; Tensile properties