title: Antioxidant and anti-ageing activities of mycelia zinc polysaccharide from Pholiota nameko SW-03.
abstract: BACKGROUND
  Edible fungi polysaccharides usually exhibit antioxidant activity, and zinc has been shown to have antioxidant properties. In the present work, Pholiota nameko SW-03 was used as a vector of zinc biotransformation in order to obtain mycelia zinc polysaccharide (MZPS), and the structural characterization and anti-ageing activity of MZPS were investigated.
  
  
  RESULTS
  Pholiota nameko SW-03 could accumulate zinc in the form of zinc-enriched polysaccharide, and the zinc content in MZPS was 16.39 ± 0.72 mg g(-1) . Three fractions (MZPS-1, MZPS-2 and MZPS-3) were successfully isolated. The main fraction (MZPS-2) with the highest antioxidant activity in vitro was composed of glucose, mannose, glucuronic acid, galactose, galacturonic acid and arabinose in a molar ratio of 172.59:5.29:4.61:4.20:1.01:1.00, with a weight-average molecular weight of 13.63 kDa. The anti-ageing capability was measured in d-galactose-induced ageing mice, and the results showed that MZPS could improve antioxidant status (superoxide dismutase, total antioxidant capability, malondialdehyde and lipid peroxide), indicating that MZPS had strong anti-ageing capability in vivo.
  
  
  CONCLUSIONS
  This study suggested that organification of zinc through edible fungi liquid fermentation provided a novel method to produce MZPS, which might be used as a natural antioxidant to slow the progression of ageing.
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Guo-Yin
authors.initials: GY
authors.lastname: Zhai
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ma
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Li-Qin
authors.initials: LQ
authors.lastname: Wang
authors.affiliation: College of Life Science, Shandong Agricultural University, Taian, 271018, China.
authors.forename: Le
authors.initials: L
authors.lastname: Jia
mesh_terms: D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology; D001711:Biotransformation; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D005690:Galactose / Q000009:adverse effects; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D008970:Molecular Weight; D025282:Mycelium / Q000737:chemistry; D055368:Pholiota / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism*; D014131:Trace Elements / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001688:Biological Products; D062610:Fungal Polysaccharides; D014131:Trace Elements; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D015032:Zinc; D005690:Galactose
keywords: Pholiota nameko SW-03; anti-ageing activity; antioxidant activity; polysaccharide; zinc