title: Application of in situ diffraction in high-throughput structure determination platforms.
abstract: Macromolecular crystallography (MX) is the most powerful technique available to structural biologists to visualize in atomic detail the macromolecular machinery of the cell. Since the emergence of structural genomics initiatives, significant advances have been made in all key steps of the structure determination process. In particular, third-generation synchrotron sources and the application of highly automated approaches to data acquisition and analysis at these facilities have been the major factors in the rate of increase of macromolecular structures determined annually. A plethora of tools are now available to users of synchrotron beamlines to enable rapid and efficient evaluation of samples, collection of the best data, and in favorable cases structure solution in near real time. Here, we provide a short overview of the emerging use of collecting X-ray diffraction data directly from the crystallization experiment. These in situ experiments are now routinely available to users at a number of synchrotron MX beamlines. A practical guide to the use of the method on the MX suite of beamlines at Diamond Light Source is given.
authors.affiliation: Diamond Light Source Ltd., Diamond House, Harwell Science and Innovation Campus, Chilton, Didcot, Oxfordshire, OX11 0DE, UK.
authors.forename: Pierre
authors.initials: P
authors.lastname: Aller
authors.forename: Juan
authors.initials: J
authors.lastname: Sanchez-Weatherby
authors.forename: James
authors.initials: J
authors.lastname: Foadi
authors.forename: Graeme
authors.initials: G
authors.lastname: Winter
authors.forename: Carina M C
authors.initials: CM
authors.lastname: Lobley
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.forename: Alun W
authors.initials: AW
authors.lastname: Ashton
authors.forename: Domenico
authors.initials: D
authors.lastname: Bellini
authors.forename: Jose
authors.initials: J
authors.lastname: Brandao-Neto
authors.forename: Simone
authors.initials: S
authors.lastname: Culurgioni
authors.forename: Alice
authors.initials: A
authors.lastname: Douangamath
authors.forename: Ramona
authors.initials: R
authors.lastname: Duman
authors.forename: Gwyndaf
authors.initials: G
authors.lastname: Evans
authors.forename: Stuart
authors.initials: S
authors.lastname: Fisher
authors.forename: Ralf
authors.initials: R
authors.lastname: Flaig
authors.forename: David R
authors.initials: DR
authors.lastname: Hall
authors.forename: Petra
authors.initials: P
authors.lastname: Lukacik
authors.forename: Marco
authors.initials: M
authors.lastname: Mazzorana
authors.forename: Katherine E
authors.initials: KE
authors.lastname: McAuley
authors.forename: Vitaliy
authors.initials: V
authors.lastname: Mykhaylyk
authors.forename: Robin L
authors.initials: RL
authors.lastname: Owen
authors.forename: Neil G
authors.initials: NG
authors.lastname: Paterson
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Romano
authors.forename: James
authors.initials: J
authors.lastname: Sandy
authors.forename: Thomas
authors.initials: T
authors.lastname: Sorensen
authors.forename: Frank
authors.initials: F
authors.lastname: von Delft
authors.forename: Armin
authors.initials: A
authors.lastname: Wagner
authors.forename: Anna
authors.initials: A
authors.lastname: Warren
authors.forename: Mark
authors.initials: M
authors.lastname: Williams
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: Martin A
authors.initials: MA
authors.lastname: Walsh
mesh_terms: D057205:Automation, Laboratory; D018360:Crystallography, X-Ray / Q000295:instrumentation* / Q000379:methods*; D046911:Macromolecular Substances / Q000737:chemistry*; D040901:Proteomics / Q000295:instrumentation / Q000379:methods; D012984:Software; D017356:Synchrotrons / Q000295:instrumentation
chemical_list: D046911:Macromolecular Substances