title: Anabolic properties of high mobility group box protein-1 in human periodontal ligament cells in vitro.
abstract: High mobility group box protein-1 (HMGB1) is mainly recognized as a chemoattractant for macrophages in the initial phase of host response to pathogenic stimuli. However, recent findings provide evidence for anabolic properties in terms of enhanced proliferation, migration, and support of wound healing capacity of mesenchymal cells suggesting a dual role of the cytokine in the regulation of immune response and subsequent regenerative processes. Here, we examined potential anabolic effects of HMGB1 on human periodontal ligament (PDL) cells in the regulation of periodontal remodelling, for example, during orthodontic tooth movement. Preconfluent human PDL cells (hPDL) were exposed to HMGB1 protein and the influence on proliferation, migration, osteogenic differentiation, and biomineralization was determined by MTS assay, real time PCR, immunofluorescence cytochemistry, ELISA, and von Kossa staining. HMGB1 protein increased hPDL cell proliferation, migration, osteoblastic marker gene expression, and protein production as well as mineralized nodule formation significantly. The present findings support the dual character of HMGB1 with anabolic therapeutic potential that might support the reestablishment of the structural and functional integrity of the periodontium following periodontal trauma such as orthodontic tooth movement.
authors.affiliation: Department of Orthodontics, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Wolf
authors.affiliation: Department of Orthodontics, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lossdörfer
authors.affiliation: Department of Orthodontics, University of Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany.
authors.forename: Piero
authors.initials: P
authors.lastname: Römer
authors.affiliation: Department of Pediatric Hematology and Oncology, Center for Pediatrics, University of Bonn Medical Center, Adenauerallee 119, 53113 Bonn, Germany.
authors.forename: Rogerio Bastos
authors.initials: RB
authors.lastname: Craveiro
authors.affiliation: Experimental Dento-Maxillo-Facial Medicine, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany.
authors.forename: James
authors.initials: J
authors.lastname: Deschner
authors.affiliation: Department of Orthodontics, University of Bonn, Welschnonnenstraße 17, 53111 Bonn, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Jäger
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000378:metabolism; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008660:Metabolism / Q000502:physiology*; D015675:Osteocalcin / Q000378:metabolism; D010513:Periodontal Ligament / Q000378:metabolism*; D012038:Regeneration; D014070:Tooth / Q000502:physiology*; D014945:Wound Healing
chemical_list: D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D015675:Osteocalcin; D000469:Alkaline Phosphatase