title: Decreased expression of fecal miR-4478 and miR-1295b-3p in early-stage colorectal cancer.
abstract: BACKGROUND
  Colorectal cancer (CRC) is a major cause of cancer-related deaths world-wide. Detection of molecular markers in stool samples is a promising strategy for CRC screening. MicroRNAs (miRNAs) are short, non-coding RNA molecules that are commonly dysregulated in neoplasia.
  
  
  OBJECTIVE
  The objective of this study was to evaluate the fecal miRNAs differentiation between early-stage CRC patients and healthy subjects.
  
  
  METHODS
  Stool samples were collected from 40 patients with early stage (I, II) CRC and 16 healthy controls. RNA was extracted from all samples using miRNAeasy Mini Kits. MiRNA microarray expression profiling was performed with Agilent's miRNA Microarray system on 12 CRC and 8 normal stool samples. The expression levels of miR-4478 and miR-1295b-3p were determined by the SYBR Green miScript PCR system.
  
  
  RESULTS
  In profiling study, we found 215 down-regulated miRNAs in CRC group. Furthermore, in validation study we found that the expression levels of fecal miR-4487 and miR-1295b-3p were significantly decreased in CRC patients compared to healthy controls.
  
  
  CONCLUSIONS
  The expression of miR-4478 and miR-1295b-3p were significantly diminished in stool samples of CRC patients with early stage (I, II) in comparison with normal group. These miRNAs maybe use as potential non-invasive molecular markers for CRC diagnosis, but further studies are needed.
authors.affiliation: Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ghanbari
authors.affiliation: Department of Pathology, Haartman Institute, Helsinki University, Helsinki, Finland.
authors.forename: Neda
authors.initials: N
authors.lastname: Mosakhani
authors.affiliation: Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Jahanbakhsh
authors.initials: J
authors.lastname: Asadi
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Nazila
authors.initials: N
authors.lastname: Nouraee
authors.affiliation: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
authors.affiliation: Liver and Pancreatobiliary Disease Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Poustchi
authors.affiliation: Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Malekzadeh
authors.affiliation: Department of Pathology, Haartman Institute, Helsinki University, Helsinki, Finland.
authors.forename: Sakari
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D012372:ROC Curve; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor; C000604651:MIRN-1295 microRNA, human; C000604650:MIRN-4478 microRNA, human; D035683:MicroRNAs
keywords: Profiling; diagnosis; microarray