title: Protective effects of Selenium-enriched probiotics on carbon tetrachloride-induced liver fibrosis in rats.
abstract: This study aimed to investigate the effects of Se-enriched probiotics (SP) on the liver fibrosis induced by CCl4 in rats. The results showed that SP significantly decreased serum alanine aminotransferase (87.0 ± 1.96 U/L), aspartate aminotransferase (101 ± 3.13 U/L), hepatic hydroxyproline (898 ± 72.5 μg/g), and malondialdehyde (2.39 ± 0.34 nmol/mg) levels, but increased glutathione peroxidase (37.2 ± 3.19 U/mg), superoxide dismutase (201 ± 19.2 U/mg), and glutathione levels (3.32 ± 0.25 mg/g) (P < 0.05) in rats treated by CCl4. SP suppressed hepatic inflammation and necrosis induced by CCl4. Moreover, SP significantly reduced the expression of α-smooth muscle actin, collagen, TGF-β1, TIMP-1, and inflammation-related gene and induced apoptosis of activated hepatic stellate cells (P < 0.05) in rats treated by CCl4. Our results suggest that SP could protect the liver from fibrosis by attenuating hepatic oxidative stress, suppressing hepatic inflammation, and inducing apoptosis of hepatic stellate cells.
authors.forename: Yunhuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Gengping
authors.initials: G
authors.lastname: Ye
authors.forename: Alamzeb
authors.initials: A
authors.lastname: Khan
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Fang
authors.initials: F
authors.lastname: Gan
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Shahnawaz
authors.initials: S
authors.lastname: Kumbhar
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000410:Alanine Transaminase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000235:genetics / Q000378:metabolism; D002251:Carbon Tetrachloride / Q000009:adverse effects; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007779:Lactobacillus acidophilus / Q000737:chemistry* / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D019936:Probiotics / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism; D020011:Protective Agents / Q000008:administration & dosage* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000008:administration & dosage* / Q000032:analysis / Q000378:metabolism
chemical_list: D020011:Protective Agents; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D012643:Selenium