title: Cytotoxicity of curcumin silica nanoparticle complexes conjugated with hyaluronic acid on colon cancer cells.
abstract: We report results of our investigations on the cytotoxic efficacy of Organically modified silica nanoparticle (SiNp)-curcumin complex conjugated with hyaluronic acid (HA) (HA-SiNp-cur) and HA free SiNp-cur complex in human colon carcinoma (colo-205) cells. Curcumin was loaded in SiNp and resulting complexes were conjugated with HA, which has a strong affinity for cancer cells expressing CD44. After conjugation with HA, the average size of the SiNp-cur nanoparticles increased from 45 nm to 70 nm, and zeta potential changed to -33 mV from -26 mV. Compared to free curcumin and SiNp-cur, curcumin in HA-SiNp was more stable. The uptake and cytotoxicity of curcumin delivered through HA-SiNp-cur was significantly higher in monolayer and spheroids as compared to free curcumin and HA free SiNp-cur. Concomitantly, HA-SiNp-cur complex treatment resulted in higher inhibition of growth and migration of cells in spheroids. Further, incubation of colo-205 cancer cells with an excess of HA impaired the uptake of HA-SiNp-cur confirming the involvement of receptor mediated endocytosis in the uptake of HA conjugated nanocomplex. Time dependent increase in the fluorescence of curcumin observed in the release media when HA-SiNp-cur was incubated with hyaluronidase suggests involvement of enzyme in release of curcumin from nanoparticle.
authors.affiliation: Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013, Madhya Pradesh, India.
authors.forename: Surya Prakash
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013, Madhya Pradesh, India. Electronic address: msharma@rrcat.gov.in.
authors.forename: Mrinalini
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore-452013, Madhya Pradesh, India.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Gupta
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003110:Colonic Neoplasms; D003474:Curcumin / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004355:Drug Stability; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D018874:Spheroids, Cellular; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D012822:Silicon Dioxide; D006820:Hyaluronic Acid; D003474:Curcumin
keywords: Colon carcinoma; Curcumin; Hyaluronic acid; Organically modified silica nanoparticle