title: Laser Capture Microdissection of Feline Streptomyces spp Pyogranulomatous Dermatitis and Cellulitis.
abstract: Suspected Streptomyces spp infections were identified in 4 cats at UC Davis Veterinary Medical Teaching Hospital between 1982 and 2011. Three had ulcerated, dark red mycetomas involving the dermis, subcutis, and fascia with fistulous tracts and/or regional lymphadenopathy. One cat had pyogranulomatous mesenteric lymphadenitis. Granulomatous inflammation in all cats contained colonies of Gram-positive, non-acid-fast organisms. All 4 cats failed to respond to aggressive medical and surgical treatment and were euthanized. Laser capture microdissection (LCM) was used to selectively harvest DNA from the affected formalin-fixed, paraffin-embedded (FFPE) tissues. Cloned amplicons from LCM-derived tissue confirmed the presence of Streptomyces spp in the dermatitis cases. Amplicons from the remaining cat with peritoneal involvement aligned with the 16S ribosomal RNA gene for Actinomycetales. Usually considered a contaminant, Streptomyces spp can be associated with refractory pyogranulomatous dermatitis and cellulitis in cats with outdoor access. LCM is useful in the diagnosis of bacterial diseases where contamination may be an issue.
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA National Institutes of Health, NINDS, Section of Infections of the Nervous System, Bethesda, MD, USA ryan.traslavina@nih.gov.
authors.forename: R P
authors.initials: RP
authors.lastname: Traslavina
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Reilly
authors.affiliation: University of Texas Health Science Center Tyler, TX, USA.
authors.forename: R
authors.initials: R
authors.lastname: Vasireddy
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Samitz
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: C T
authors.initials: CT
authors.lastname: Stepnik
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: C
authors.initials: C
authors.lastname: Outerbridge
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: V K
authors.initials: VK
authors.lastname: Affolter
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Byrne
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Lowenstine
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: White
authors.affiliation: School of Veterinary Medicine University of California, Davis, CA, USA.
authors.forename: B
authors.initials: B
authors.lastname: Murphy
mesh_terms: D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000382:microbiology* / Q000473:pathology; D002415:Cats; D002481:Cellulitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003872:Dermatitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D061346:Laser Capture Microdissection / Q000662:veterinary*; D008297:Male; D008969:Molecular Sequence Data; D016612:Paraffin Embedding / Q000662:veterinary; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D013302:Streptomyces / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Actinomycetales; Streptomyces; cats; cellulitis; dermatitis; laser capture microdissection; mycetoma