title: Cloning of porcine GPIHBP1 gene and its tissue expression pattern and genetic effect on adipose traits.
abstract: Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism and is transported by glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1) from the interstitial spaces to the capillary lumen. Here, we cloned a cDNA and the genomic locus of the porcine GPIHBP1 gene, and investigated its tissue expression pattern and its genetic effects on adipose traits. Porcine GPIHBP1 exhibits a four-exon/three-intron structure, including a 543bp open reading frame that encodes 180 amino acids. The porcine GPIHBP1 protein shows 49%-65% homology and shares the major conserved structural domains of GPIHBP1 proteins in other mammals. Porcine GPIHBP1 mRNA levels were high in the adipose tissue, muscle and lung, and higher mRNA levels were observed in sows compared to boars in adipose tissues of the inner and outer layers of subcutaneous fat, abdominal fat, and suet fat. The mRNA expression pattern of porcine GPIHBP1 and LPL genes was similar in most tissues except for the lung. Thirty six single nucleotide polymorphisms (SNPs) were found in the porcine GPIHBP1 gene. Association analyses showed that the g.-255G>C and g.-626T>G SNPs are associated with intramuscular fat content, and that the g.-1557T>C and g.-1948G>A SNPs are associated with back fat thickness. In conclusion, porcine GPIHBP1 mRNA is abundantly expressed in the adipose tissue, muscle and lung, and gender affects GPIHBP1 mRNA expression levels; furthermore, four GPIHBP1 SNPs are genetic factors affecting adipose traits.
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Huaming
authors.initials: H
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Tao
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Xing
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Wangmin
authors.initials: W
authors.lastname: Cen
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Anxiang
authors.initials: A
authors.lastname: Wen
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Tang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an City 625014, China.
authors.forename: Anan
authors.initials: A
authors.lastname: Jiang
authors.affiliation: College of Life Sciences, Sichuan Agricultural University, Ya'an City 625014, China. Electronic address: jiangyz04@163.com.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an City 625014, China. Electronic address: xuewei.li@sicau.edu.cn.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
mesh_terms: D050154:Adiposity; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001947:Breeding; D003001:Cloning, Molecular; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D056726:Genetic Association Studies; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D050151:Subcutaneous Fat / Q000502:physiology*; D034421:Sus scrofa
chemical_list: D012333:RNA, Messenger; D018110:Receptors, Lipoprotein
keywords: Cloning; Glycosylphosphatidylinositol-anchored high-density lipoprotein-binding protein 1 (GPIHBP1); Lipoprotein lipase (LPL); Polymorphism; Porcine; Tissue expression