title: Nano-encapsulation of coenzyme Q10 using octenyl succinic anhydride modified starch.
abstract: Octenyl succinic anhydride modified starch (OSA-ST) was used to encapsulate coenzyme Q10 (CoQ10). CoQ10 was dissolved in rice bran oil and incorporated into an aqueous OSA-ST solution. High pressure homogenisation of the mixture was conducted at 170 MPa for 56 cycles. The resulting emulsion had a particle size range of 200-300 nm and the absolute zeta potential varied between 8.4 and 10.6 mV. CoQ10 retention of the emulsion and freeze dried products, determined by a hexane rinse, was 98.2%. Reconstitution of the freeze dried product in Mcllvaine citrate-phosphate buffers with pH values of 3-5 and temperatures at 4 and 25 °C had very little effect on the range and distribution of the nanoparticles' size. The inflection point of the zeta potential and pH plot occurred at the first pKa of succinic acid (pH 4.2), indicating succinate as the main influence over zeta potential.
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: Sherwin Y
authors.initials: SY
authors.lastname: Cheuk
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: Frederick F
authors.initials: FF
authors.lastname: Shih
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: Elaine T
authors.initials: ET
authors.lastname: Champagne
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: Kim W
authors.initials: KW
authors.lastname: Daigle
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: James A
authors.initials: JA
authors.lastname: Patindol
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA.
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Mattison
authors.affiliation: Agricultural Research Service-Southern Regional Research Center, U.S. Department of Agriculture, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA. Electronic address: steve.boue@ars.usda.gov.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Boue
mesh_terms: D049329:Nanostructures / Q000737:chemistry*; D013213:Starch / Q000031:analogs & derivatives* / Q000737:chemistry; D013387:Succinic Anhydrides / Q000737:chemistry*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D013387:Succinic Anhydrides; C551966:octenyl succinic anhydride-modified starch; D014451:Ubiquinone; D013213:Starch; C024989:coenzyme Q10
keywords: Coenzyme Q10; High pressure homogenisation; Modified starch; Nano-emulsion; Nano-encapsulation; Octenyl succinic anhydride; Supercritical fluid extraction; Zeta potential