title: The effect of stabilizer on the mechanical response of double-emulsion-templated polymersomes.
abstract: Recent studies have shown that polymersomes templated by microfluidic double-emulsion possess several advantages such as high monodispersity and encapsulation efficiency compared with those generated based on thin-film rehydration and electroformation. Stabilizers, including bovine serum albumin (BSA) and polyvinyl alcohol (PVA), have been used to enhance the formation and stability of double emulsions that are used as templates for the generation of polymersomes. In this work, the effect of stabilizers on the mechanical response of double-emulsion-templated polymersomes using micropipette aspiration is investigated. It is demonstrated that the existence of stabilizers results in the inelastic response in poly-mersomes in the early stage of solvent removal. However, aged polymersomes that have little residual solvent show elastic behavior. Polymersomes prepared from PVA-stabilized double emulsions have noticeably lower area expansion moduli than polymersomes prepared from stabilizer-free and BSA-stabilized double emulsions, suggesting that PVA is incorporated in the bilayer membrane of polymersomes.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Pennsylvania, 220 South 33rd Street 311A Towne Building, Philadelphia, PA, 19104-6315, USA.
authors.forename: Woo-Sik
authors.initials: WS
authors.lastname: Jang
authors.forename: Seung Chul
authors.initials: SC
authors.lastname: Park
authors.forename: Miju
authors.initials: M
authors.lastname: Kim
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
authors.forename: Daeyeon
authors.initials: D
authors.lastname: Lee
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Hammer
mesh_terms: D000818:Animals; D002417:Cattle; D004655:Emulsions / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques; D009821:Oils / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D013997:Time Factors
chemical_list: D004655:Emulsions; D009821:Oils; D011108:Polymers; D012710:Serum Albumin, Bovine; D011142:Polyvinyl Alcohol
keywords: bovine serum albumin; microfluidic double emulsion; micropipette aspiration; polymersome; polyvinyl alcohol