title: Capsaicin inhibits collagen fibril formation and increases the stability of collagen fibers.
abstract: Capsaicin is a versatile plant product which has been ascribed several health benefits and anti-inflammatory and analgesic properties. We have investigated the effect of capsaicin on the molecular stability, self-assembly, and fibril stability of type-I collagen. It was found that capsaicin suppresses collagen fibril formation, increases the stability of collagen fibers in tendons, and has no effect on the molecular stability of collagen. Turbidity assay data show that capsaicin does not promote disassembly of collagen fibrils. However, capsaicin moderately protects collagen fibrils from enzymatic degradation. Computational studies revealed the functions of the aromatic group and amide region of capsaicin in the collagen-capsaicin interaction. The results may have significant implications for capsaicin-based therapeutics that target excess collagen accumulation-linked pathology, for example thrombosis, fibrosis, and sclerosis.
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai, 600020, Tamil Nadu, India.
authors.forename: Sathiamurthi
authors.initials: S
authors.lastname: Perumal
authors.forename: Kriti
authors.initials: K
authors.lastname: Dubey
authors.forename: Rahul
authors.initials: R
authors.lastname: Badhwar
authors.forename: Kodimattan Joseph
authors.initials: KJ
authors.lastname: George
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Sharma
authors.forename: Ganesh
authors.initials: G
authors.lastname: Bagler
authors.forename: Balaraman
authors.initials: B
authors.lastname: Madhan
authors.forename: Karunakar
authors.initials: K
authors.lastname: Kar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002211:Capsaicin / Q000737:chemistry / Q000494:pharmacology*; D024022:Fibrillar Collagens / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D011485:Protein Binding; D055550:Protein Stability; D059748:Proteolysis; D051381:Rats; D017208:Rats, Wistar
chemical_list: D024022:Fibrillar Collagens; D002211:Capsaicin