title: The first CD73-instructed supramolecular hydrogel.
abstract: Enzymatic supramolecular hydrogelation is a simple, controllable, and novel strategy for preparation of soft colloidal materials, which allows the integration of self-assembly with enzyme associated biological processes. The development of more enzymes involve in hydrogelation is a subject of developing useful soft colloids. In this work, an ectoenzyme, CD73, was found to trigger the formation of nanofibers as matrices of hydrogels. CD73 is an important cell surface enzyme which converts extracellular adenosine monophosphate (AMP) to adenosine. It is broadly expressed in many cancer cells and participates in tumor growth. The successful application of CD73 in self-assembly and hydrogelation may provide new strategies for CD73-guided materials and therapies.
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Du
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA. Electronic address: bxu@brandeis.edu.
authors.forename: Bing
authors.initials: B
authors.lastname: Xu
mesh_terms: D015720:5'-Nucleotidase / Q000737:chemistry / Q000378:metabolism*; D000241:Adenosine / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D002470:Cell Survival; D058851:GPI-Linked Proteins / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism*; D061986:MCF-7 Cells; D057139:Nanofibers / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D058851:GPI-Linked Proteins; D020100:Hydrogels; D000249:Adenosine Monophosphate; D015720:5'-Nucleotidase; C548241:NT5E protein, human; D000241:Adenosine
keywords: AMP; CD73; Enzyme; Hydrogel