title: Kinetics of circulating levels of miR-195, miR-155 and miR-21 in patients with breast cancer undergoing mastectomy.
abstract: BACKGROUND
  MicroRNAs are small RNA molecules that negatively regulate the expression of the majority of proteins, mainly at the post-transcriptional level. Being stable in the circulation and resistant to storage handling, they are potentially promising biomarkers.
  
  
  METHODS
  We measured RNA levels of three microRNAs with tumorigenic or angiogenic potential (miR-155, miR-195, and miR-21) in blood samples taken from patients with early breast cancer, both preoperatively and postoperatively.
  
  
  RESULTS
  We found that persistently elevated postoperative levels of miR-195 were detected only in patients who developed early tumor relapse and that miR-155 levels tended to increase three days postoperatively (p=0.05) and fell below baseline one month post-surgery (p<0.05). We had no major findings for miR-21.
  
  
  CONCLUSIONS
  The results of this pilot study indicate a possible involvement of miR-155 in surgery-induced angiogenesis and potential prognostic significance of high postoperative levels of circulating miR-195 in patients with breast cancer.
authors.affiliation: Cancer Biobank Center, University of Ioannina, Ioannina, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Igglezou
authors.affiliation: Cancer Biobank Center, University of Ioannina, Ioannina, Greece Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece.
authors.forename: Katerina
authors.initials: K
authors.lastname: Vareli
authors.affiliation: Department of Surgery, University Hospital of Ioannina, Ioannina, Greece.
authors.forename: Georgios K
authors.initials: GK
authors.lastname: Georgiou
authors.affiliation: Cancer Biobank Center, University of Ioannina, Ioannina, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Sainis
authors.affiliation: Cancer Biobank Center, University of Ioannina, Ioannina, Greece ebriasou@otenet.gr.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Briasoulis
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D008408:Mastectomy; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009389:Neovascularization, Pathologic / Q000097:blood*; D010865:Pilot Projects; D011184:Postoperative Period; D057234:Preoperative Period
chemical_list: D014408:Biomarkers, Tumor; C524942:MIRN155 microRNA, human; C552722:MIRN195 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs
keywords: Breast cancer; biomarkers; miR-155; miR-195; miR-21