title: Metabonomic analysis of Bombyx mori (Heterocera: Bombysidae) treated with acetaminophen.
abstract: The feasibility of using Bombyx mori as model animal is attracting more attention. Whether the effect of drugs on the metabolite profiling was consistent with those in mammals was an aspect to evaluate the feasibility of B. mori as model animal. In this study, we used acetaminophen to treat Dazao fifth-instar B. mori, and its metabolites in hemolymph were detected by gas chromatography-mass spectrometry. The corresponding data were processed and analyzed by total model analysis, principal component analysis, partial least squares-discriminant analysis, orthogonal partial least squares-discriminant analysis, and finally, the difference metabolites between acetaminophen group and control group were selected and identified by our reference material database and the National Institute of Standard and Technology database. The results showed that acetaminophen administration induced elevation of metabolites related to energy source, the intermediate of cholesterol synthesis, and the metabolites related to melanization and also induced the decrease of metabolites in pathway of Krebs cycle, the cholesterol, and sitosterol, which suggested that acetaminophen administration inhibited energy metabolism and promoted the expenditure and imbalance of hormone and melanization.
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: W M
authors.initials: WM
authors.lastname: Yin
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: X
authors.initials: X
authors.lastname: Xu
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: Y
authors.initials: Y
authors.lastname: He
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China.
authors.forename: G B
authors.initials: GB
authors.lastname: Wei
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Sima
authors.affiliation: School of Biology & Basic Medical Science, Medical College, Soochow University, Suzhou 215123, China National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China szsqxu@suda.edu.cn.
authors.forename: Xu
authors.initials: X
authors.lastname: Shi-Qing
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology*; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D012831:Bombyx / Q000187:drug effects* / Q000378:metabolism; D002784:Cholesterol / Q000096:biosynthesis; D002952:Citric Acid Cycle / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D006458:Hemolymph / Q000378:metabolism; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008543:Melanins / Q000378:metabolism; D055442:Metabolome; D055432:Metabolomics / Q000379:methods / Q000706:statistics & numerical data; D012855:Sitosterols / Q000378:metabolism
chemical_list: D018712:Analgesics, Non-Narcotic; D008543:Melanins; D012855:Sitosterols; D000082:Acetaminophen; C025473:gamma-sitosterol; D002784:Cholesterol
keywords: Bombyx mori; Krebs cycle; acetaminophen; horme synthesis; metabolite