title: Performance of the 47-kilodalton membrane protein versus DNA polymerase I genes for detection of Treponema pallidum by PCR in ulcers.
abstract: Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.
authors.affiliation: Division of Clinical Epidemiology, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland angele.gayet-ageron@hcuge.ch.
authors.forename: Angèle
authors.initials: A
authors.lastname: Gayet-Ageron
authors.affiliation: Laboratory of Bacteriology, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
authors.affiliation: Bacteriology Laboratory and Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland Genomic Research Laboratory, University of Geneva Hospitals, Geneva, Switzerland.
authors.forename: Jacques
authors.initials: J
authors.lastname: Schrenzel
authors.affiliation: Laboratory of Bacteriology, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Charton
authors.affiliation: Bacteriology Laboratory and Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland.
authors.forename: Gisela
authors.initials: G
authors.lastname: Jimenez-Getaz
authors.affiliation: Genomic Research Laboratory, University of Geneva Hospitals, Geneva, Switzerland.
authors.forename: Manuela
authors.initials: M
authors.lastname: Tangomo
authors.affiliation: Service de Maladies Infectieuses et Tropicales, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Ferry
authors.affiliation: Institut Alfred Fournier, Laboratoire CNR Gonocoques, Paris, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Sednaoui
authors.affiliation: Triemlispital, Dermatologisches Ambulatorium Triemli, Zürich, Switzerland.
authors.forename: Stephan
authors.initials: S
authors.lastname: Lautenschlager
authors.affiliation: Department of Dermatology and Venereology, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
authors.forename: Laurence
authors.initials: L
authors.lastname: Toutous-Trellu
authors.affiliation: Department of Obstetrics and Gynaecology, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
authors.forename: Begoña
authors.initials: B
authors.lastname: Martinez de Tejada
authors.affiliation: Service de Maladies Infectieuses, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Cavassini
authors.affiliation: Bacteriology Laboratory and Division of Infectious Diseases, University of Geneva Hospitals, Geneva, Switzerland.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Emonet
authors.affiliation: Division of Clinical Epidemiology, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Perneger
authors.affiliation: Laboratory of Bacteriology, Hôpital de la Croix-Rousse, Hospices Civils de Lyon, Lyon, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Salord
mesh_terms: D000328:Adult; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D004256:DNA Polymerase I / Q000032:analysis* / Q000235:genetics; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000032:analysis* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D013587:Syphilis / Q000175:diagnosis* / Q000382:microbiology; D014210:Treponema pallidum / Q000235:genetics / Q000302:isolation & purification*; D014456:Ulcer / Q000382:microbiology*
chemical_list: C090573:47-kDa immunogen, Treponema pallidum; D002352:Carrier Proteins; D008074:Lipoproteins; D004256:DNA Polymerase I