title: Effective clean-up and ultra high-performance liquid chromatography-tandem mass spectrometry for isoflavone determination in legumes.
abstract: Legumes are an excellent source of macronutrients and phytochemicals as isoflavones. The aim of this work was to develop a new analytical method for determining five isoflavone compounds, three of which are aglycons, namely daidzein, genistein, biochanin A, and two of which, daidzin and genistin, are glycosilated, in lentils and other pulses, using an effective clean-up system and UHPLC-MS/MS (triple quadrupole) method. The recoveries obtained by spiking the lentil samples with a standard mixture of isoflavones at three levels of fortification (5, 25 and 100 μg kg(-1)) were in the range of 54.4-111.1%, 68.6-91.1%, and 84.4-114%, respectively. The method was applied to analyse 48 lentil samples from central Italy and pulses for determining the isoflavone content, which was found to range from 1.1 to 95.6 μg kg(-1).
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Pilar
authors.initials: P
authors.lastname: Vila-Donat
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Filippo
authors.initials: F
authors.lastname: Maggi
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Torregiani
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy.
authors.forename: Sauro
authors.initials: S
authors.lastname: Vittori
authors.affiliation: School of Pharmacy, University of Camerino, Via Sant'Agostino 1, 62032 Camerino, Italy. Electronic address: gianni.sagratini@unicam.it.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods; D005615:Freezing; D007529:Isoflavones / Q000032:analysis* / Q000737:chemistry; D048789:Phytoestrogens / Q000032:analysis / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007529:Isoflavones; D048789:Phytoestrogens
keywords: Clean-up; Isoflavones; Legumes; Phytoestrogens; SPE-UHPLC–MS/MS