title: A review: fabrications, detections and applications of peptide nucleic acids (PNAs) microarray.
abstract: Peptide nucleic acid (PNA) is a mimic of DNA that shows a high chemical stability and can survive the enzymatic degradation of nucleases and proteases. The superior binding properties of PNA enable the formation of PNA/DNA or PNA/RNA duplex with excellent thermal stability and unique ionic strength effect. The introduction of microarray makes it possible to achieve accurate, high throughput parallel analysis of DNA or RNA with a highly integrated and low reagents consuming device. This powerful tool expands the applications of PNA in genotyping based on single nucleotide polymorphism (SNP) detection, the monitoring of disease-related miRNA expression and pathogen detection. This review paper discusses the fabrications of PNA microarrays through in situ synthesis strategy or spotting method by automatic devices, the various detection methods for the microarray-based hybridization and the current applications of PNA microarrays.
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Shi
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Feipeng
authors.initials: F
authors.lastname: Yang
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Kaixuan
authors.initials: K
authors.lastname: Nie
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha 410083, China. Electronic address: liuzhengchunseu@126.com.
authors.forename: Zhengchun
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D013058:Mass Spectrometry; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D018716:Molecular Mimicry; D009693:Nucleic Acid Hybridization; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D014780:Viruses / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D020135:Peptide Nucleic Acids
keywords: Application; DNA; Detection; Fabrication; Microarray; PNA