title: Ionic starch-based hydrogels for the prevention of nonspecific protein adsorption.
abstract: Non-fouling materials bind water molecules via either hydrogen bonding or ionic solvation to form a hydration layer which is responsible for their resistance to protein adsorption. Three ionic starch-based polymers, namely a cationic starch (C-Starch), an anionic starch (A-Starch) and a zwitterionic starch (Z-Starch), were synthesized via etherification reactions to incorporate both hydrogen bonding and ionic solvation hydration groups into one molecule. Further, C-, A- and Z-Starch hydrogels were prepared via chemical crosslinking. The non-fouling properties of these hydrogels were tested with different proteins in solutions with different ionic strengths. The C-Starch hydrogel had low protein resistance at all ionic strengths; the A-Starch hydrogel resisted protein adsorption at ionic strengths of more than 10mM; and the Z-Starch hydrogel resisted protein adsorption at all ionic strengths. In addition, the A- and Z-Starch hydrogels both resisted cell adhesion. This work provides a new path for developing non-fouling materials using the integration of polysaccharides with anionic or zwitterionic moieties to regulate the protein resistance of materials.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Basic Medical Sciences, Hebei United University, Tangshan 063000, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Science, Beijing 100850, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Dianyu
authors.initials: D
authors.lastname: Dong
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China. Electronic address: yaofanglian@tju.edu.cn.
authors.forename: Fanglian
authors.initials: F
authors.lastname: Yao
mesh_terms: D000327:Adsorption; D006735:Horseradish Peroxidase / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry; D009994:Osmolar Concentration; D010434:Pepsin A / Q000737:chemistry*; D013213:Starch / Q000031:analogs & derivatives / Q000737:chemistry*
chemical_list: D020100:Hydrogels; C051538:IGG-horseradish peroxidase; D007074:Immunoglobulin G; D013213:Starch; D006735:Horseradish Peroxidase; D009113:Muramidase; D010434:Pepsin A
keywords: Hydrogel; Hydrogen bonding hydration; Ionic solvation hydration; Non-fouling; Starch; Zwitterionic