title: Compact multi-enzyme pathways in P. pastoris.
abstract: We report for the first time the functional simultaneous expression of nine genes from a single 2A peptide based polycistronic expression construct. The feasibility and arising opportunities for the biosynthetic pathway balancing for chemical production were demonstrated by the co-expression of the violacein and carotenoid biosynthesis pathways.
authors.affiliation: Austrian Centre of Industrial Biotechnology (ACIB GmbH), Petersgasse 14, 8010 Graz, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Geier
authors.forename: Pia
authors.initials: P
authors.lastname: Fauland
authors.forename: Thomas
authors.initials: T
authors.lastname: Vogl
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002338:Carotenoids / Q000378:metabolism; D004798:Enzymes / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D007211:Indoles / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics; D010843:Pichia / Q000201:enzymology* / Q000235:genetics
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D007211:Indoles; D002338:Carotenoids; C063155:violacein