title: Upregulated genes at 2q24 gains as candidate oncogenes in hepatoblastomas.
abstract: OBJECTIVE
  Cytogenetic data of hepatoblastomas, a rare embryonal tumor of the liver, mostly consist of descriptions of whole-chromosome aneuploidies and large chromosome alterations. High-resolution cytogenetics may provide clues to hepatoblastoma tumorigenesis and indicate markers with clinical significance.
  
  
  METHODS
  We used array-CGH (180K) to screen for genomic imbalances in nine hepatoblastomas. Additionally, we investigated the expression pattern of selected genes exhibiting copy number changes.
  
  
  RESULTS
  Analysis showed mainly whole-chromosome or chromosome-arm aneuploidies, but some focal aberrations were also mapped. Expression analysis of 48 genes mapped at one 10 Mb amplification at 2q24 revealed upregulation of DAPL1, ERMN, GALNT5, SCN1A and SCN3A in the set of tumors compared with differentiated livers.
  
  
  CONCLUSIONS
  These genes appear as candidates for hepatoblastoma tumorigenesis.
authors.affiliation: Department of Genetics & Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Tatiane Cristina
authors.initials: TC
authors.lastname: Rodrigues
authors.forename: Felipe
authors.initials: F
authors.lastname: Fidalgo
authors.forename: Cecilia Maria Lima
authors.initials: CM
authors.lastname: da Costa
authors.forename: Elisa Napolitano
authors.initials: EN
authors.lastname: Ferreira
authors.forename: Isabela Werneck
authors.initials: IW
authors.lastname: da Cunha
authors.forename: Dirce Maria
authors.initials: DM
authors.lastname: Carraro
authors.forename: Ana Cristina Victorino
authors.initials: AC
authors.lastname: Krepischi
authors.forename: Carla
authors.initials: C
authors.lastname: Rosenberg
mesh_terms: D000782:Aneuploidy; D002869:Chromosome Aberrations; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D018197:Hepatoblastoma / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D009857:Oncogenes; D012189:Retrospective Studies; D015854:Up-Regulation
keywords: 2q24 amplification; array-CGH; copy number aberration; embryonal tumor; gene expression; hepatoblastoma