title: Association between Angiotensinogen M235T Polymorphism and Preeclampsia in Iranian Pregnant Women.
abstract: OBJECTIVE
  To determine the possible association between the M235T variant of angiotensinogen gene and preeclampsia in Iranian preeclamtic women with hypertension during pregnancy.
  
  
  METHODS
  During a case control study, we used polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis to investigate the association between M235T polymorphism in preeclamtic women compared to normotensive controls.
  
  
  RESULTS
  The M235T polymorphism was significantly associated with increased preeclampsia risk in the studied population as supported by a p value of 0.017 and chi-square value of 8.12. The frequency of mutated allele and genotype distribution showed a significant difference between preeclamtic women and control groups.
  
  
  CONCLUSIONS
  The result indicates that the AGT M235T polymorphism plays a significant role in preeclampsia observed in selected Iranian preeclamtic women, and it can be considered as a major risk factor for preeclampsia.
authors.affiliation: Department of Public Health, School of Health and Nutrition, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Raha
authors.initials: R
authors.lastname: Afshariani
authors.affiliation: Urology Nephrology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Jamshid
authors.initials: J
authors.lastname: Roozbeh
authors.affiliation: Department of Neurology, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Sharifian
authors.affiliation: Urology Nephrology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mahbobeh
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Department of Obstetrics and Gynecology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Alamtaj
authors.initials: A
authors.lastname: Samsami Dehaghani
authors.affiliation: Shiraz Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
keywords: Angiotensinogen M235T Polymorphism; Preeclampsia