title: Comparison of chromosome analysis using cell culture by coverslip technique with flask technique.
abstract: OBJECTIVE
  To determine accuracy rate ofchromosome study from amniotic cellculture by coverslip technique compared with flask technique and to compared timing ofamniotic cell culture, amount ofamniotic cell culture media and cost ofamniotic cell culture.
  
  
  METHODS
  Cross sectional study.
  
  
  METHODS
  Department of Obstetrics and Gynecology, Phramongkutklao Hospital. Subjects: 70 pregnant women who underwent amniocentesis at Phramongkutklao Hospital during November 1, 2007 to February 29, 2008.
  
  
  METHODS
  Amniotic cell culture by flask technique and coverslip technique.
  
  
  METHODS
  Accuracy of amniotic cell culture for chromosome study by coverslip technique compared with flask technique.
  
  
  RESULTS
  Totally 70 pregnant women who underwent to amniocentesis and dividedamniotic fluid to cell culture by flask technique and coverslip technique. 69 samples had similar resultfrom both techniques. The only one sample had cell culture failure inboth methods due to blood contamination. Accuracy in coverslip technique was 100% compared with flask technique. In timing of amniotic cell culture, amount ofamniotic cell culture media and cost of amniotic cell culture between 2 methods that coverslip technique was lesser than flask technique.
  
  
  CONCLUSIONS
  There is statistically significant of accuracy in chromosome result between coverslip technique and flask technique. Coverslip technique was lesser than flask technique in timing, amniotic cell culture media and costs ofamniotic cell culture.
authors.forename: Suraphan
authors.initials: S
authors.lastname: Sajapala
authors.forename: Kitti
authors.initials: K
authors.lastname: Buranawut
authors.forename: Md
authors.initials: M
authors.lastname: NiwatArunyakasemsuk
mesh_terms: D000328:Adult; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002478:Cells, Cultured; D025063:Chromosome Disorders / Q000175:diagnosis*; D002877:Chromosomes, Human / Q000737:chemistry; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D011247:Pregnancy; D011264:Pregnancy Trimesters; D011296:Prenatal Diagnosis / Q000379:methods*; D012680:Sensitivity and Specificity