title: Hemodynamic forces regulate developmental patterning of atrial conduction.
abstract: Anomalous action potential conduction through the atrial chambers of the heart can lead to severe cardiac arrhythmia. To date, however, little is known regarding the mechanisms that pattern proper atrial conduction during development. Here we demonstrate that atrial muscle functionally diversifies into at least two heterogeneous subtypes, thin-walled myocardium and rapidly conducting muscle bundles, during a developmental window just following cardiac looping. During this process, atrial muscle bundles become enriched for the fast conduction markers Cx40 and Nav1.5, similar to the precursors of the fast conduction Purkinje fiber network located within the trabeculae of the ventricles. In contrast to the ventricular trabeculae, however, atrial muscle bundles display an increased proliferation rate when compared to the surrounding myocardium. Interestingly, mechanical loading of the embryonic atrial muscle resulted in an induction of Cx40, Nav1.5 and the cell cycle marker Cyclin D1, while decreasing atrial pressure via in vivo ligation of the vitelline blood vessels results in decreased atrial conduction velocity. Taken together, these data establish a novel model for atrial conduction patterning, whereby hemodynamic stretch coordinately induces proliferation and fast conduction marker expression, which in turn promotes the formation of large diameter muscle bundles to serve as preferential routes of conduction.
authors.affiliation: Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, United States of America.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Bressan
authors.affiliation: Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, United States of America.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Louie
authors.affiliation: Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, United States of America.
authors.forename: Takashi
authors.initials: T
authors.lastname: Mikawa
mesh_terms: D000200:Action Potentials; D000818:Animals; D016275:Atrial Function / Q000502:physiology; D002642:Chick Embryo; D006321:Heart / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D006325:Heart Atria; D006329:Heart Conduction System / Q000502:physiology*; D006439:Hemodynamics / Q000502:physiology; D008954:Models, Biological