title: [Study on identification of Sarcandra glabra and Chloranthus spicatus's leaves by PCR amplification of specific alleles].
abstract: The paper is aimed to identify SNP in Sarcandra glabra and Chloranthus spicatus, and authenticate S. glabra from Ch. spicatus and the mixture by using PCR amplification of specific alleles. SNPs in the ITS sequences of S. glabra and Ch. spicatus were found by ClustulX 2. 1 program and Bioedit software. Primers for authentic S. glabra and Ch. spicatus was designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system, and then optimized the PCR reaction system. Five hundred and eighty band special for S. glabra and 470 bp band special for Ch. spicatus were found by using multi-PCR reaction. The multi-PCR reaction system could be applied to identify S. glabra and Ch. spicatus's leaves.
authors.forename: Yi-cong
authors.initials: YC
authors.lastname: Wei
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Lin-quan
authors.initials: LQ
authors.lastname: Luo
authors.forename: Qun-xiong
authors.initials: QX
authors.lastname: Yang
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Chen
authors.forename: Yi-chi
authors.initials: YC
authors.lastname: Liang
authors.forename: Su-Rong
authors.initials: SR
authors.lastname: Chen
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D019684:Magnoliopsida / Q000145:classification / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012335:RNA, Ribosomal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C065096:RNA, ribosomal, 26S