title: A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide.
abstract: Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.
authors.affiliation: Department of Chemistry, University of Massachusetts Boston, 100 Morrissey Boulevard, Boston, Massachusetts 02125, USA. jonathan.rochford@umb.edu.
authors.forename: S
authors.initials: S
authors.lastname: Bag
authors.forename: J-C
authors.initials: JC
authors.lastname: Tseng
authors.forename: J
authors.initials: J
authors.lastname: Rochford
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D051379:Mice; D008819:Mice, Nude; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013154:Spleen / Q000737:chemistry* / Q000166:cytology; D013481:Superoxides / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D013481:Superoxides; D008165:Luminol