title: Efficiency of an automatic dehydrated carrier for the vitrification of human embryos derived from three pronuclei fertilized zygotes.
abstract: The efficiency of a new vitrification technique using embryos obtained after abnormal fertilization was assesed. A total of 100 embryos were vitrified, 48 with the automatic dehydrated carrier (ADC) technique and 52 with cryotop. The survival rates in ADC and cryotop groups were similar (85.4% versus 92.3%). The loss rates of 10.4% for the ADC group and 1.9% for the cryotop group were not significantly different. The intact rate was significantly lower in the ADC group (26.8% versus 64.6%; P = 0.001). After 48 h of culture, blastocyst formation rates in the ADC and cryotop groups were 14.6% and 25.0%, respectively, which were not significantly different. In conclusion, the practicability of ADC vitrification was demonstrated. The study has some limitations, which necessitates further work.
authors.affiliation: Chong Qing Reproductive and Genetic Institute, 64 Jing Tang ST, Yu Zhong District, Chong Qing 400013, China. Electronic address: zhangxd207@gmail.com.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chong Qing Reproductive and Genetic Institute, 64 Jing Tang ST, Yu Zhong District, Chong Qing 400013, China; Chong Qing Obstetric and Gynecology Hospital, 64 Jing Tang ST, Yu Zhong District, Chong Qing 400013, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Chong Qing Reproductive and Genetic Institute, 64 Jing Tang ST, Yu Zhong District, Chong Qing 400013, China.
authors.forename: Guoning
authors.initials: G
authors.lastname: Huang
mesh_terms: D001331:Automation; D001755:Blastocyst / Q000187:drug effects; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry; D004867:Equipment Design; D005260:Female; D005307:Fertilization in Vitro / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D009865:Oocytes / Q000166:cytology; D010062:Ovulation Induction; D020554:Sperm Injections, Intracytoplasmic; D013997:Time Factors; D058989:Vitrification; D015053:Zygote / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents
keywords: blastocyst formation; new vitrification technique; vitrification