title: Chimeric neuraminidase and mutant PB1 gene constellation improves growth and yield of H5N1 vaccine candidate virus.
abstract: We previously showed that a mutated PB1 gene improved the growth kinetics of a H3N2 influenza reassortant. Here, we showed that the same mutations improved the growth kinetics of a virus containing the A/Vietnam/1203/2004 (H5N1) haemagglutinin and neuraminidase (NA). Total protein yield and NA activity were increased when a chimeric NA was included. These increases indicated that the synergistic effect was due to the gene constellation containing both the altered PB1 gene and the chimeric NA gene.
authors.affiliation: Laboratory of Respiratory Viral Diseases, Division of Viral Products, Office of Vaccine Research and Review, US Food and Drug Administration, Silver Spring, MD 20993, USA.
authors.forename: Ewan P
authors.initials: EP
authors.lastname: Plant
authors.affiliation: Laboratory of Respiratory Viral Diseases, Division of Viral Products, Office of Vaccine Research and Review, US Food and Drug Administration, Silver Spring, MD 20993, USA.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Ye
mesh_terms: D000818:Animals; D002460:Cell Line; D018920:Coculture Techniques; D004285:Dogs; D057809:HEK293 Cells; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development* / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics* / Q000276:immunology; D061985:Madin Darby Canine Kidney Cells; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D006388:Hemagglutinins; D007252:Influenza Vaccines; D014764:Viral Proteins; C077346:influenza virus polymerase basic protein 1; D009439:Neuraminidase