title: Antibacterial effect of ultrafine nanodiamond against gram-negative bacteria Escherichia coli.
abstract: We investigate the antibacterial effect of ultrafine nanodiamond particles with an average size of 5 nm against the gram-negative bacteria Escherichia coli (E. coli). UV-visible, Raman spectroscopy, and scanning electron microscopy (SEM) have been employed to elucidate the nature of the interaction. The influence on bacterial growth was monitored by measuring optical densities of E. coli at 600 nm as a function of time in the presence of carboxylated nanodiamond (cND) particles (100 μg/ml ) in highly nutritious liquid Luria-Bertani medium. The SEM images prove that cND particles are attached to the bacterial cell wall surface and some portion of the bacterial cell wall undergoes destruction. Due to the change of the protein structure on the bacterial wall, a small Raman shift in the region of 1400 to 1700 cm⁻¹ was observed when E. coli interacted with cNDs. Raman mapping images show strong evidence of cND attachment at the bacterial cell wall surface. Electrotransformation of E. coli with a fluorescent protein markers experiment demonstrated that the interaction mechanisms are different for E. coli treated with cND particles, E. coli by lysozyme treatment, and E. coli that suffer lysis.
authors.forename: Anindita
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Elena
authors.initials: E
authors.lastname: Perevedentseva
authors.forename: Mona
authors.initials: M
authors.lastname: Jani
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Cheng
authors.forename: Ying-Siou
authors.initials: YS
authors.lastname: Ye
authors.forename: Pei-Hua
authors.initials: PH
authors.lastname: Chung
authors.forename: Chia-Liang
authors.initials: CL
authors.lastname: Cheng
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D006090:Gram-Negative Bacteria / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000737:chemistry; D058612:Nanodiamonds / Q000737:chemistry*; D036103:Nanotechnology; D055095:Optics and Photonics; D013059:Spectrum Analysis, Raman
chemical_list: D000900:Anti-Bacterial Agents; D058612:Nanodiamonds; D049452:Green Fluorescent Proteins; D009113:Muramidase