title: Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance.
abstract: Several plant organs contain proteinase inhibitors, which are produced during normal plant development or are induced upon pathogen attack to suppress the enzymatic activity of phytopathogenic microorganisms. In this study, we examined the presence of proteinase inhibitors, specifically trypsin inhibitors, in the leaf extract of Capsicum baccatum var. pendulum inoculated with PepYMV (Pepper yellow mosaic virus). Leaf extract from plants with the accession number UENF 1624, which is resistant to PepYMV, was collected at 7 different times (0, 24, 48, 72, 96, 120, and 144 h). Seedlings inoculated with PepYMV and control seedlings were grown in a growth chamber. Protein extract from leaf samples was partially purified by reversed-phase chromatography using a C2/C18 column. Residual trypsin activity was assayed to detect inhibitors followed by Tricine-SDS-PAGE analysis to determine the N-terminal peptide sequence. Based on trypsin inhibitor assays, trypsin inhibitors are likely constitutively synthesized in C. baccatum var. pendulum leaf tissue. These inhibitors are likely a defense mechanism for the C. baccatum var. pendulum- PepYMV pathosystem.
authors.affiliation: Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil moniquemoulin@gmail.com.
authors.forename: M M
authors.initials: MM
authors.lastname: Moulin
authors.affiliation: Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Rodrigues
authors.affiliation: Laboratório de Fisiologia e Bioquímica de Microorganismos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil.
authors.forename: S F F
authors.initials: SF
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Melhoramento Genético Vegetal, Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: L S A
authors.initials: LS
authors.lastname: Gonçalves
authors.affiliation: Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil.
authors.forename: C S
authors.initials: CS
authors.lastname: Bento
authors.affiliation: Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil.
authors.forename: C P
authors.initials: CP
authors.lastname: Sudré
authors.affiliation: Laboratório de Toxinas Vegetais, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
authors.forename: I M
authors.initials: IM
authors.lastname: Vasconcelos
authors.affiliation: Laboratório de Fisiologia e Bioquímica de Microorganismos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brasil.
authors.forename: V M
authors.initials: VM
authors.lastname: Gomes
mesh_terms: D000595:Amino Acid Sequence; D002212:Capsicum / Q000276:immunology / Q000821:virology*; D056148:Chromatography, Reverse-Phase; D060467:Disease Resistance / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000502:physiology*; D010935:Plant Diseases / Q000276:immunology* / Q000821:virology*; D010936:Plant Extracts / Q000378:metabolism; D018515:Plant Leaves / Q000821:virology*; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010936:Plant Extracts; D014361:Trypsin Inhibitors