title: The roles of C-terminal residues on the thermal stability and local heme environment of cytochrome c' from the thermophilic purple sulfur bacterium Thermochromatium tepidum.
abstract: A soluble cytochrome (Cyt) c' from thermophilic purple sulfur photosynthetic bacterium Thermochromatium (Tch.) tepidum exhibits marked thermal tolerance compared with that from the closely related mesophilic counterpart Allochromatium vinosum. Here, we focused on the difference in the C-terminal region of the two Cyts c' and examined the effects of D131 and R129 mutations on the thermal stability and local heme environment of Cyt c' by differential scanning calorimetry (DSC) and resonance Raman (RR) spectroscopy. In the oxidized forms, D131K and D131G mutants exhibited denaturing temperatures significantly lower than that of the recombinant control Cyt c'. In contrast, R129K and R129A mutants denatured at nearly identical temperatures with the control Cyt c', indicating that the C-terminal D131 is an important residue maintaining the enhanced thermal stability of Tch. tepidum Cyt c'. The control Cyt c' and all of the mutants increased their thermal stability upon the reduction. Interestingly, D131K exhibited narrow DSC curves and unusual thermodynamic parameters in both redox states. The RR spectra of the control Cyt c' exhibited characteristic bands at 1,635 and 1,625 cm(-1), ascribed to intermediate spin (IS) and high spin (HS) states, respectively. The IS/HS distribution was differently affected by the D131 and R129 mutations and pH changes. Furthermore, R129 mutants suggested the lowering of their redox potentials. These results strongly indicate that the D131 and R129 residues play significant roles in maintaining the thermal stability and modulating the local heme environment of Tch. tepidum Cyt c'.
authors.affiliation: Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Nada, Kobe, 657-8501, Japan, ykimura@people.kobe-u.ac.jp.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kimura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kasuga
authors.forename: Masashi
authors.initials: M
authors.lastname: Unno
authors.forename: Takashi
authors.initials: T
authors.lastname: Furusawa
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Osoegawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohno
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Wang-Otomo
mesh_terms: D002152:Calorimetry, Differential Scanning; D002841:Chromatiaceae / Q000378:metabolism*; D018360:Crystallography, X-Ray; D045305:Cytochromes c' / Q000737:chemistry* / Q000378:metabolism*; D006418:Heme / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism; D011489:Protein Denaturation; D055550:Protein Stability; D013059:Spectrum Analysis, Raman; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D045305:Cytochromes c'; D050505:Mutant Proteins; D006418:Heme