title: Dispersive Raman spectroscopy and multivariate data analysis to detect offal adulteration of thawed beefburgers.
abstract: Beef offal (i.e., kidney, liver, heart, lung) adulteration of beefburgers was studied using dispersive Raman spectroscopy and multivariate data analysis to explore the potential of these analytical tools for detection of adulterations in comminuted meat products with complex formulations. Adulterated (n = 46) and authentic (n = 36) beefburger samples were produced based on formulations derived using market knowledge and an experimental design. Raman spectral data in the fingerprint range (900-1800 cm(-1)) were examined using both a classification (partial least-squares discriminant analysis, PLS-DA) and class-modeling (soft independent modeling of class analogy, SIMCA) approach to identify offal-adulterated and authentic beefburgers. PLS-DA models correctly classified 89-100% of authentic and 90-100% of adulterated samples. SIMCA models were developed using either PCA or PLS scores as input data. For authentic beefburgers, they exhibited sensitivity, specificity, and efficiency values of 0.94-1, 0.64-1, and 0.80-0.97, respectively. PLS regression quantitative models were also developed in an attempt to quantify total offal and added fat in these samples. The performance of PLS regression quantitative models for prediction of added fat may be acceptable for screening purposes, with the most accurate model producing a coefficient of determination in prediction of 0.85 and a root-mean-square error of prediction equal to 3.8% w/w.
authors.affiliation: Teagasc Food Research Centre Ashtown , Ashtown, Dublin 15, Ireland.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Gerard
authors.initials: G
authors.lastname: Downey
authors.forename: Colm P
authors.initials: CP
authors.lastname: O'Donnell
mesh_terms: D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008461:Meat Products / Q000032:analysis*; D013059:Spectrum Analysis, Raman / Q000379:methods*
keywords: Raman; beefburgers; classification; discrimination; offal