title: Characterization of novel transcripts of human papillomavirus type 16 using cap analysis gene expression technology.
abstract: We have performed cap-analysis gene expression (CAGE) sequencing to identify the regulatory networks that orchestrate genome-wide transcription in human papillomavirus type 16 (HPV16)-positive cervical cell lines of different grades: W12E, SiHa, and CaSki. Additionally, a cervical intraepithelial neoplasia grade 1 (CIN1) lesion was assessed for identifying the transcriptome expression profile. Here we have precisely identified a novel antisense noncoding viral transcript in HPV16. In conclusion, CAGE sequencing should pave the way for understanding a diversity of viral transcript expression.
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Taguchi
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan nagasakak-tky@umin.ac.jp kkawana-tky@umin.org.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Nagasaka
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan nagasakak-tky@umin.ac.jp kkawana-tky@umin.org.
authors.forename: Kei
authors.initials: K
authors.lastname: Kawana
authors.affiliation: RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Pathogen Genomic Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Kusumoto-Matsuo
authors.affiliation: RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Japan.
authors.forename: Charles
authors.initials: C
authors.lastname: Plessy
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
authors.forename: Miranda
authors.initials: M
authors.lastname: Thomas
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiroe
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Japan.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Bonetti
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Oda
authors.affiliation: Pathogen Genomic Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Iwao
authors.initials: I
authors.lastname: Kukimoto
authors.affiliation: RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Japan.
authors.forename: Piero
authors.initials: P
authors.lastname: Carninci
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
authors.forename: Lawrence
authors.initials: L
authors.lastname: Banks
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Osuga
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Fujii
mesh_terms: D005260:Female; D020869:Gene Expression Profiling; D015967:Gene Expression Regulation, Viral; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008967:Molecular Biology / Q000379:methods*; D016372:RNA, Antisense / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D022661:RNA, Untranslated / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012367:RNA, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification*; D002583:Uterine Cervical Neoplasms / Q000821:virology
chemical_list: D016372:RNA, Antisense; D022661:RNA, Untranslated; D012367:RNA, Viral