title: Anti-allergic, anti-inflammatory and antioxidant activities of the different extracts of Thai traditional remedy called prabchompoothaweep for allergic rhinitis treatment.
abstract: BACKGROUND
  Prabchompoothaweep remedy (PT) has long been used in Thai traditional medicine to treat allergic rhinitis and asthma. It is composed of 23 plants. It is on National herbal drug list of Thailand, but there is no reportfor anti-allergic, anti-inflammatory and antioxidant activities.
  
  
  OBJECTIVE
  To investigate anti-allergic, anti-inflammatory and antioxidant activities of the crude extract from PTby different extraction method.
  
  
  METHODS
  The method of extract used was maceration in 95% ethanol and 50% ethanol; the residue of these extracts were continued extracted by boiling water, they obtained PTE95, PTE50, PTR95 andPTR50, respectively. The other method of extraction was boiling and drying by lyophilizer that obtained PTW Five crude extracts were determined anti- allergic activity by the inhibition of β-hexosaminidase release from RBL-2H3 cell lines, anti-inflammatory activity were determined by the inhibition ofnitric oxide (NO) production from RA W264. 7 cell lines induced by lipopolysaccharide (LPS) and antioxidant activity were tested by DPPH radical scavenging assay.
  
  
  RESULTS
  PTE95 showed the most potent ofanti-allergic activity, anti-inflammatory activity and antioxidant activity (IC5 = 12.97, 22.51 and EC50 = 14.62 μg/ml, respectively).
  
  
  CONCLUSIONS
  These results suggest that the method of extraction PT that showed the best anti-allergy, anti-inflamation and antioxidant activity was maceration in 95% ethanol.
authors.forename: Areeratna
authors.initials: A
authors.lastname: Jai-aue
authors.forename: Sunita
authors.initials: S
authors.lastname: Makchuchit
authors.forename: Thana
authors.initials: T
authors.lastname: Juckmeta
authors.forename: Arunporn
authors.initials: A
authors.lastname: Itharat
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001491:Basophils / Q000187:drug effects* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008519:Medicine, Traditional; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D051381:Rats; D065631:Rhinitis, Allergic / Q000188:drug therapy*; D013785:Thailand; D001619:beta-N-Acetylhexosaminidases / Q000187:drug effects / Q000378:metabolism
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D009569:Nitric Oxide; D001619:beta-N-Acetylhexosaminidases