title: Evaluation of the progression and treatment of experimental periodontitis in rats subjected to chemotherapy with 5-fluorouracil.
abstract: OBJECTIVE
  This study aimed to evaluate the progression and treatment of experimental periodontitis (EP) in rats treated with 5-fluorouracil (5-FU).
  
  
  METHODS
  One-hundred fifty rats were divided into groups: C--control, EP--EP induction, EP/scaling and root planing (SRP)--EP induction and SRP, 5-FU--systemic treatment with 5-FU, 5-FU/EP--systemic treatment with 5-FU and EP induction, and 5-FU/EP/SRP--systemic treatment with 5-FU, EP induction and SRP. Systemic treatment consisted of the administration of 80 and 40 mg/kg of 5-FU. The animals were euthanised at 7, 15 and 30 days. Histological and immunohistochemical analyses for the detection of the receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP) were performed at the furcation region. A histometric analysis was performed to evaluate the percentage of bone in the furcation region (PBF).
  
  
  RESULTS
  In the 5-FU/EP and 5-FU/EP/SRP groups, there was less PBF, greater breakdown of periodontal tissues and increased immunostaining for RANKL compared with the EP and EP/SRP groups.
  
  
  CONCLUSIONS
  It can be concluded that 5-FU aggravates PE progression, impairs the host response to SRP mechanical therapy and does not seem to cause changes in the healthy periodontium.
authors.affiliation: Department of Surgery and Integrated Clinic-Division of Periodontics, Univ Estadual Paulista (UNESP), Araçatuba, São Paulo, Brazil, vg.garcia@uol.com.br.
authors.forename: Valdir Gouveia
authors.initials: VG
authors.lastname: Garcia
authors.forename: Vivian Cristina Noronha
authors.initials: VC
authors.lastname: Novaes
authors.forename: Juliano Milanezi
authors.initials: JM
authors.lastname: de Almeida
authors.forename: Mariéllen
authors.initials: M
authors.lastname: Longo
authors.forename: Edilson
authors.initials: E
authors.lastname: Ervolino
authors.forename: Suely Regina Mogami
authors.initials: SR
authors.lastname: Bomfim
authors.forename: Leticia Helena
authors.initials: LH
authors.lastname: Theodoro
mesh_terms: D016301:Alveolar Bone Loss / Q000628:therapy; D000818:Animals; D012534:Dental Scaling; D018450:Disease Progression; D005472:Fluorouracil / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D010518:Periodontitis / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D016745:Root Planing
chemical_list: D005472:Fluorouracil