title: Upregulated expression of miR-106a by DNA hypomethylation plays an oncogenic role in hepatocellular carcinoma.
abstract: Aberrant microRNA (miRNA) expression has been widely recognized to play an extremely important role in several cancers, including hepatocellular carcinoma (HCC). According to the previous studies, abnormal miR-106a expression was closely related to various cancer occurrences. However, the miR-106a expression in HCC remains unclear. In our study, we firstly detected the miR-106a expression levels in 36 pairs of HCC tissues. The results showed that miR-106a expression in HCC tissues was apparently higher than the level in the adjacent tissues. Then, we used quantitative real-time PCR (qPCR) and BSP to analyze miR-106a expression and promoter methylation in HCC cell lines. There came to a conclusion that the methylation status of the miR-106a promoter region was inversely correlated with the expression of miR-106a. After prediction with online software, we further used dual-luciferase reporter gene assay to ensure that TP53INP1 and CDKN1A might be the direct targets of miR-106a. At last, we explored the functions of miR-106a in HCC cells in vitro. Our results manifested that high-miR-106a cell line had stronger invasiveness, faster cell cycle progression, and more resistance to apoptosis compared with the low-miR-106a cell line. Therefore, our study suggested that upregulated expression of miR-106a by its promoter hypomethylation might contribute to the progression of HCC, which might be considered as a potentially effective biomarker and therapeutic approach in the future.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, 188# Shizi Road, Suzhou, 215006, China.
authors.forename: Renshun
authors.initials: R
authors.lastname: Yuan
authors.forename: Qiaoming
authors.initials: Q
authors.lastname: Zhi
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Ye
authors.initials: Y
authors.lastname: Han
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Kou
authors.forename: Zhaoji
authors.initials: Z
authors.lastname: Guo
authors.forename: Songbing
authors.initials: S
authors.lastname: He
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xue
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
mesh_terms: D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; C542541:MIRN106 microRNA, human; D035683:MicroRNAs