title: [Discussion on polysaccharide determination methods in new Chinese drug research].
abstract: According to existing problems in polysaccharide determination methods in new Chinese drug applications, the method suitability, chemical reference selection, components interference and method research were introduced. The author suggests that suitable determination method should be selected according to the structure and property of the polysaccharide, and validated. Some influent factors should be examined to assure the accuracy of the method, such as the stability and using amount of the visualizing reagent, visualizing time, maximum detection wavelength etc. Monosaccharide and other water soluble components should be removed from polysaccharide sample, and suitable reference substance and detection wavelength should be selected. It should pay attention to mutual interference of neutral and acidic saccharide, and use inhibitor to eliminate the interference. Because the slopes of the standard curves are different for different monosaccharide, it is proposed that the types and ratios of the monosaccharide in heteroglycan should be understood, and mixed reference substance solution in the ratio is prepared for determination.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Li
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D009005:Monosaccharides / Q000032:analysis* / Q000302:isolation & purification; D011134:Polysaccharides / Q000032:analysis* / Q000302:isolation & purification; D012106:Research / Q000639:trends; D013678:Technology, Pharmaceutical / Q000379:methods* / Q000639:trends
chemical_list: D004365:Drugs, Chinese Herbal; D009005:Monosaccharides; D011134:Polysaccharides