title: Non-selective and selective enrichment media for the recovery of Clostridium difficile from chopped beef.
abstract: Clostridium difficile exists within the intestines of animals and in meat products. Enrichment of C. difficile in an appropriate medium is necessary for the detection of C. difficile in meat products. Non-selective media (brain heart infusion medium [TBHI] and cooked meat medium containing sodium taurocholate [TCM]) and selective media (cycloserine-cefoxitin-fructose medium [TCCFB] and C. difficile moxalactam-norfloxacin medium containing antibiotics and sodium taurocholate [TCDMN]) can be used to enrich C. difficile. This study aimed to evaluate non-selective and selective enrichment media for the recovery of C. difficile from beef specimens. The efficiency of the enrichment media was investigated on the basis of the recovery frequency of C. difficile from beef specimens inoculated with C. difficile. The beef specimens were inherently contaminated with bacteria (around 10(4)CFUg(-1)), and further inoculated with C. difficile (around 10(0)CFUg(-1)). The antibiotics in TCCFB and TCDMN adversely affected C. difficile growth. The bacteria inherent to these specimens exhibited resistance to antibiotics and grew during the enrichment of C. difficile-inoculated chopped beef in TCCFB and TCDMN, which hindered the recovery of C. difficile. The frequency of recovery of C. difficile from beef specimens in TCM was higher than that from any other enrichment medium.
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea.
authors.forename: Changhoon
authors.initials: C
authors.lastname: Chai
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea.
authors.forename: Kyung-Soo
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea.
authors.forename: Dayoung
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea.
authors.forename: Soyeon
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea. Electronic address: swoh@kookmin.ac.kr.
authors.forename: Se-Wook
authors.initials: SW
authors.lastname: Oh
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D002417:Cattle; D016360:Clostridioides difficile / Q000302:isolation & purification*; D003470:Culture Media / Q000737:chemistry*; D008460:Meat / Q000382:microbiology*
chemical_list: D003470:Culture Media
keywords: Antibiotics; Beef; Clostridium difficile; Cooked meat medium; Enrichment medium