title: Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers.
abstract: Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 20m; 2n = 20 = 18m + 2sm; 2n = 20 = 16m + 4sm; and 2n = 20 = 12m + 8sm. The results of our ISSR analysis revealed the genetic relationships among the 27 cultivars; these relationships were analyzed by using the unweighted pair-group method based on DNA polymorphism. Our results revealed that all of the native cultivars showed considerable genetic diversity. At a genetic distance of 0.324, the 27 varieties could be classified into five categories; this grouping corresponded well with the chromosome formulas. All of the investigated hemp cultivars represent relatively primitive types; moreover, the genetic distances show a geographical distribution, with a small amount of regional hybridity.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China.
authors.forename: L G
authors.initials: LG
authors.lastname: Zhang
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, China yingchangnew@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Chang
authors.affiliation: College of Mathematics and Information Sciences of Guangxi University, Nanning, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: F Z
authors.initials: FZ
authors.lastname: Guan
authors.affiliation: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: H M
authors.initials: HM
authors.lastname: Yuan
authors.affiliation: Institute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Crop Breeding, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Zhao
mesh_terms: D002188:Cannabis / Q000235:genetics* / Q000254:growth & development; D002681:China; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D010802:Phylogeny
chemical_list: D005819:Genetic Markers