title: [Effective Components of three kinds of shen-supplementing Chinese medicine on self-renewal and neuron-like differentiation of NSCs in AD mouse embryos: an experimental research].
abstract: OBJECTIVE
  To observe the regulatory effects of psoralen, oleanolic acid, and stilbene glucoside, three active components of psoralea fruit, glossy privet fruit and tuber fleeceflower root respectively, on Aβ25-35induced self-renewal and neuron-like differentiation of neural stem cells (NSCs).
  
  
  METHODS
  Embryonic NSCs werein vitro isolated and cultured from Kunming mice of 14-day pregnancy, and randomly divided into the control group, the Aβ25-35 group, the Aβ25-35 +psoralen group, the Aβ25-35 +oleanolic acid group, and the Aβ25-35 + stilbene glucoside group. The intervention concentration of Aβ25-35 was 25 µmol/L, and the intervention concentration of three active components of Chinese medicine was 10(-7)mol/L. The effect of three active components of Chinese medicine on the proliferation of NSCs was observed by counting method. The protein expression of Tubulin was observed by Western blot and immunofluorescence. The ratio of Tubulin+/DAPI was caculated. Results Compared with the control group, the sperical morphology of NSCs was destroyed in the Aβ25-35 group, the counting of NSCs, the expression of Tubulin protein, and the ratio of Tubulin /DAPI all decreased (P <0.01, P <0.05). Compared with the Aβ25-35 group, the counting of NSCs, the expression of Tubulin protein, and the ratio of Tubulin + /DAPI all increased in the three Chinese medicine treated groups (P <0. 01, P <0. 05).
  
  
  CONCLUSIONS
  25 µmol/L Aβ25-35 could inhibit self-renewal and neuron-like differentiating of NSCs. But psoralen, oleanolic acid, and stilbene glucoside could promote self-renewal of NSCs and neuron-like differentiation.
authors.forename: Yu-lian
authors.initials: YL
authors.lastname: Zhang
authors.forename: Lin-lin
authors.initials: LL
authors.lastname: Zhang
authors.forename: Wan-shan
authors.initials: WS
authors.lastname: Song
authors.forename: Wen-wen
authors.initials: WW
authors.lastname: Han
authors.forename: Jian-hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D016229:Amyloid beta-Peptides / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D004622:Embryo, Mammalian; D005260:Female; D051379:Mice; D058953:Neural Stem Cells; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000166:cytology; D010446:Peptide Fragments / Q000502:physiology*; D011247:Pregnancy
chemical_list: D016229:Amyloid beta-Peptides; D004365:Drugs, Chinese Herbal; D010446:Peptide Fragments; C074636:amyloid beta-protein (25-35)