title: Recovery of Proteins Affected by Mobile Phase Trifluoroacetic Acid Concentration in Reversed-Phase Chromatography.
abstract: It was found that recoveries of proteins depend on trifluoroacetic acid concentration in the mobile phase and showed maximum in the range of 0.01-0.1 v/v%. Transferrin and lysozyme were used to evaluate the recoveries of proteins from dedicated reversed-phase columns. Different types of reversed-phase columns were evaluated, such as core shell type materials (Aeris Widepore with C4, C8 and C18 modification) as well as fully porous hybrid particles (Waters BEH, modified with C4 and C18 alkyl chains). Recoveries ranged between 60.7-95.2% for transferrin and 72.1-99.8% for lysozyme. Based on the data presented, at least two different adsorption effects, the well-known hydrophobic and silanophilic/polar interaction might influence the recovery. In addition to this, conformational effects due to ion pairing with the acidic mobile phase additive might change them.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest 1111, Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Bobály
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest 1111, Hungary.
authors.forename: Vivien
authors.initials: V
authors.lastname: Mikola
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest 1111, Hungary.
authors.forename: Enikő
authors.initials: E
authors.lastname: Sipkó
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest 1111, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Márta
authors.affiliation: Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, Budapest 1111, Hungary fekete@mail.bme.hu.
authors.forename: Jenő
authors.initials: J
authors.lastname: Fekete
mesh_terms: D000327:Adsorption; D000818:Animals; D002645:Chickens; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000302:isolation & purification*; D016062:Porosity; D014168:Transferrin / Q000302:isolation & purification*; D014269:Trifluoroacetic Acid / Q000737:chemistry*
chemical_list: D014168:Transferrin; D014269:Trifluoroacetic Acid; D009113:Muramidase