title: Graphene oxide single sheets as substrates for high resolution cryoTEM.
abstract: CryoTEM is an important tool in the analysis of soft matter, where generally defocus conditions are used to enhance the contrast in the images, but this is at the expense of the maximum resolution that can be obtained. Here, we demonstrate the use of graphene oxide single sheets as support for the formation of 10 nm thin films for high resolution cryoTEM imaging, using DNA as an example. With this procedure, the overlap of objects in the vitrified film is avoided. Moreover, in these thin films less background scattering occurs and as a direct result, an increased contrast can be observed in the images. Hence, imaging closer to focus as compared with conventional cryoTEM procedures is achieved, without losing contrast. In addition, we demonstrate an ~1.8 fold increase in resolution, which is crucial for accurate size analysis of nanostructures.
authors.affiliation: Laboratory of Materials and Interface Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands. N.Sommerdijk@tue.nl.
authors.forename: Marcel W P
authors.initials: MW
authors.lastname: van de Put
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Patterson
authors.forename: Paul H H
authors.initials: PH
authors.lastname: Bomans
authors.forename: Neil R
authors.initials: NR
authors.lastname: Wilson
authors.forename: Heiner
authors.initials: H
authors.lastname: Friedrich
authors.forename: Rolf A T M
authors.initials: RA
authors.lastname: van Benthem
authors.forename: Gijsbertus
authors.initials: G
authors.lastname: de With
authors.forename: Rachel K
authors.initials: RK
authors.lastname: O'Reilly
authors.forename: Nico A J M
authors.initials: NA
authors.lastname: Sommerdijk
mesh_terms: D020285:Cryoelectron Microscopy / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods*; D010087:Oxides / Q000737:chemistry
chemical_list: D010087:Oxides; D006108:Graphite