title: Effect of a surfactant on the antimicrobial activity of sodium hypochlorite solutions.
abstract: The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean®, a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Marcos Rodolfo
authors.initials: MR
authors.lastname: Bolfoni
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Marcelo dos Santos
authors.initials: Mdos S
authors.lastname: Ferla
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Otávio da Silva
authors.initials: Oda S
authors.lastname: Sposito
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: Giardino
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Rogério de Castilho
authors.initials: Rde C
authors.lastname: Jacinto
authors.affiliation: Department of Semiology and Clinics, Dental School, UFPel - Federal University of Pelotas, Pelotas, RS, Brazil.
authors.forename: Fernanda Geraldes
authors.initials: FG
authors.lastname: Pappen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000494:pharmacology; D003786:Dental Pulp Cavity / Q000382:microbiology; D013293:Enterococcus faecalis / Q000187:drug effects / Q000254:growth & development; D006801:Humans; D008422:Materials Testing; D011108:Polymers / Q000494:pharmacology; D011409:Propylene Glycols / Q000494:pharmacology; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000008:administration & dosage / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D012973:Sodium Hypochlorite / Q000008:administration & dosage / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D002593:Cetrimonium Compounds; D011108:Polymers; D011409:Propylene Glycols; D012388:Root Canal Irrigants; D013501:Surface-Active Agents; C012504:polypropylene glycol; D012973:Sodium Hypochlorite; D000077286:Cetrimonium