title: Identification of a novel gene fusion (BMX-ARHGAP) in gastric cardia adenocarcinoma.
abstract: BACKGROUND
  Gastric cardia adenocarcinoma (GCA) is one of the major causes of cancer related mortality worldwide. We aim to provide new understanding in the pathogenesis of GCA through investigations on gene expression alterations.
  
  
  METHODS
  We preformed RNA-Seq for one pair of GCA and matched non-tumor tissues. Differentially expressed genes (DEGs) and fusion genes were acquired. PCR and gel analysis in additional 14 pairs of samples were performed to validate the chimeric transcripts.
  
  
  RESULTS
  1590 up-regulated and 709 down-regulated genes were detected. Functional analysis revealed that these DEGs were significantly overrepresented in gene ontology items of cell cycle, tumor invasion and proliferation. Moreover, we firstly discovered 3 fusion genes in GCA, including BMX-ARHGAP, LRP5- LITAF and CBX3-C15orf57. The chimeric transcript BMX-ARHGAP was validated and recurrently occurred in 4/15 independent tumor tissues.
  
  
  CONCLUSIONS
  Our results may provide new understanding of GCA and biomarkers for further therapeutic studies.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_218.
authors.affiliation: Clinical Laboratory, People's hospital, Jingjiang, 214500, Jiangsu, China. 592412678@qq.com.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, People's hospital, Jingjiang, 214500, Jiangsu, China. hujianda558@gmail.com.
authors.forename: Lifang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of General Surgery, People's hospital, Jingjiang, 214500, Jiangsu, China. wuyanan864@gmail.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Department of General Surgery, People's hospital, Jingjiang, 214500, Jiangsu, China. wangjj9666@163.com.
authors.forename: Jianjiang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Clinical Laboratory, People's hospital, Jingjiang, 214500, Jiangsu, China. zhengxiaoyun860@gmail.com.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Ye
authors.affiliation: Clinical Laboratory, People's hospital, Jingjiang, 214500, Jiangsu, China. mingxianzhou@tom.com.
authors.forename: Mingxian
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory, People's hospital, Jingjiang, 214500, Jiangsu, China. liutingbo658@gmail.com.
authors.forename: Yunling
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Clinical Laboratory, People's hospital, Jingjiang, 214500, Jiangsu, China. lantao14@tom.com.
authors.forename: Lan
authors.initials: L
authors.lastname: Tao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D002299:Cardia / Q000737:chemistry* / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D050939:Gene Fusion; D056726:Genetic Association Studies; D006801:Humans; D008297:Male; D050596:Oncogene Fusion / Q000235:genetics; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012333:RNA, Messenger / Q000235:genetics; D015203:Reproducibility of Results; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger