title: Gene expression profile of increased heart rate in shensongyangxin-treated bradycardia rabbits.
abstract: Aims. The present study tries to investigate the gene expression profile of bradycardia rabbits' hearts after SSYX (SSYX, a traditional Chinese medicine) treatment. Methods. Eighteen adult rabbits were randomly assigned in three groups: sham, model, and SSYX treatment groups. Heart rate was recorded in rabbits and total RNA was isolated from hearts. Gene expression profiling was conducted and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was performed to confirm the gene expression results. Patch clamp using human induced pluripotent stem cell-derived cardiomyocytes was applied to record the calcium current in the presence of SSYX. Results. The mean RR interval reduced after six weeks due to the injury of the sinoatrial node in the model group. This effect was partially reversed by 4-week SSYX treatment. cDNA microarray demonstrated that genes related with pacemaker current, calcium ion homeostasis, and signaling were altered by SSYX treatment. Results from patch clamp demonstrated that SSYX reduced the calcium current which is consistent with gene expression results. Conclusion. The present study shows mRNA remodeling of bradycardia and demonstrates that SSYX is effective in treating bradycardia by reversing altered gene expression in bradycardia models. Reduced calcium current by SSYX also confirmed the gene expression results.
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Zhouying
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Roumu
authors.identifier: 0000-0001-9428-6609
authors.initials: R
authors.lastname: Hu
authors.affiliation: Integration of Traditional and Western Medical Research Academy of Hebei Province, Shijiazhuang 050035, China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Huo
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Jing
authors.identifier: 0000-0002-5061-4401
authors.initials: J
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Yinhui
authors.identifier: 0000-0002-8126-773X
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Integration of Traditional and Western Medical Research Academy of Hebei Province, Shijiazhuang 050035, China.
authors.forename: Cong
authors.identifier: 0000-0003-4660-6916
authors.initials: C
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Translational Cardiovascular Medicine, Fuwai Hospital & Cardiovascular Institute, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei-Li-Shi Road, Xi-Cheng District, Beijing 100037, China.
authors.forename: Jielin
authors.identifier: 0000-0002-6762-9554
authors.initials: J
authors.lastname: Pu