title: Interaction of crown ether-annelated styryl dyes with double-stranded DNA.
abstract: DNA-binding properties of 15-crown-5-derived mono- and bis-styryl dyes were investigated in the presence of calf thymus DNA. To access the factors that influence the DNA association in the series of these ligands, the structure of the molecules was varied by either changing size of the heterocyclic moiety or altering the position of the styryl substituents. The major binding mode for the monostyryl dyes is intercalation. Notably, binding of the dyes to the nucleic acids leads to a fluorescence enhancement by a factor of up to 54. Therefore, these cationic styryl derivatives may be applied as fluorescent "light-up" probes for DNA detection.
authors.affiliation: A. N. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow, Russia; Department Chemie-Biologie, Universität Siegen, Organische Chemie II, Siegen, Germany.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Berdnikova
authors.forename: Olga A
authors.initials: OA
authors.lastname: Fedorova
authors.forename: Elena V
authors.initials: EV
authors.lastname: Tulyakova
authors.forename: Haixing
authors.initials: H
authors.lastname: Li
authors.forename: Sarah
authors.initials: S
authors.lastname: Kölsch
authors.forename: Heiko
authors.initials: H
authors.lastname: Ihmels