title: The anoctamin family channel subdued mediates thermal nociception in Drosophila.
abstract: Calcium-permeable and thermosensitive transient receptor potential (TRP) channels mediate the nociceptive transduction of noxious temperature in Drosophila nociceptors. However, the underlying molecular mechanisms are not completely understood. Here we find that Subdued, a calcium-activated chloride channel of the Drosophila anoctamin family, functions in conjunction with the thermo-TRPs in thermal nociception. Genetic analysis with deletion and the RNAi-mediated reduction of subdued show that subdued is required for thermal nociception in nociceptors. Further genetic analysis of subdued mutant and thermo-TRP mutants show that they interact functionally in thermal nociception. We find that Subdued expressed in heterologous cells mediates a strong chloride conductance in the presence of both heat and calcium ions. Therefore, our analysis suggests that Subdued channels may amplify the nociceptive neuronal firing that is initiated by thermo-TRP channels in response to thermal stimuli.
authors.affiliation: From the Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Yongbong-Dong, Gwangju-Si, 500-757, South Korea.
authors.forename: Wijeong
authors.initials: W
authors.lastname: Jang
authors.affiliation: From the Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Yongbong-Dong, Gwangju-Si, 500-757, South Korea, the Aging Research Center, Korea Research Institute of Bioscience and BioTechnology, 125 Gwahak-ro, Yuseong-gu, Daejon, South Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: the School of Life Sciences, National Leading Research Laboratory for Ion Channel, and.
authors.forename: Shanyu
authors.initials: S
authors.lastname: Cui
authors.affiliation: From the Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Yongbong-Dong, Gwangju-Si, 500-757, South Korea.
authors.forename: Juyeon
authors.initials: J
authors.lastname: Jo
authors.affiliation: the School of Life Sciences, Cell Dynamic Research Center, Gwangju Institute of Science and Technology, Gwangju, 500-712, South Korea, and.
authors.forename: Byoung-Cheol
authors.initials: BC
authors.lastname: Lee
authors.affiliation: the School of Life Sciences, Cell Dynamic Research Center, Gwangju Institute of Science and Technology, Gwangju, 500-712, South Korea, and.
authors.forename: Yeonwoo
authors.initials: Y
authors.lastname: Lee
authors.affiliation: the Aging Research Center, Korea Research Institute of Bioscience and BioTechnology, 125 Gwahak-ro, Yuseong-gu, Daejon, South Korea.
authors.forename: Ki-Sun
authors.initials: KS
authors.lastname: Kwon
authors.affiliation: the School of Life Sciences, National Leading Research Laboratory for Ion Channel, and Cell Dynamic Research Center, Gwangju Institute of Science and Technology, Gwangju, 500-712, South Korea, and cspark@gist.ac.kr.
authors.forename: Chul-Seung
authors.initials: CS
authors.lastname: Park
authors.affiliation: From the Hormone Research Center, School of Biological Sciences and Technology, Chonnam National University, Yongbong-Dong, Gwangju-Si, 500-757, South Korea, changgk@chonnam.ac.kr.
authors.forename: Changsoo
authors.initials: C
authors.lastname: Kim
mesh_terms: D000818:Animals; D001522:Behavior, Animal; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000737:chemistry; D003001:Cloning, Molecular; D029721:Drosophila Proteins / Q000502:physiology*; D004331:Drosophila melanogaster / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D059225:Nociception; D009619:Nociceptors / Q000502:physiology*; D010146:Pain; D034622:RNA Interference; D050051:Transient Receptor Potential Channels / Q000502:physiology
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D029721:Drosophila Proteins; C000597012:Subdued protein, Drosophila; D050051:Transient Receptor Potential Channels
keywords: Drosophila Genetics; Genetics; Neurobiology; Neuron; Pain; Transient Receptor Potential Channels (TRP Channels)