title: Curcumin inhibits proliferation of gastric cancer cells by impairing ATP-sensitive potassium channel opening.
abstract: BACKGROUND
  This study was aimed to investigate whether ATP-sensitive potassium channel (KATP) is involved in curcumin's anti-proliferative effects against gastric cancer.
  
  
  METHODS
  In an in vitro study, gastric cancer cell line SGC-7901 was treated with curcumin at serial concentrations and co-administrated with the KATP opener, diazoxide. The effect of curcumin and diazoxide on proliferation were assessed by MTT assay. Mitochondrial membrane potential (MMP) was studied by flow cytometry detection of rhodamine 123 staining. Apoptosis was evaluated by flow cytometry detection of Annexin V propidium iodide double staining. In an in vivo study, SGC-7901 cells were planted into nude mice as xenografts. Animals were treated with curcumin co-administered with diazoxide. Tumor volume and tumor weight were observed.
  
  
  RESULTS
  Curcumin incubation significantly induced loss of MMP in SGC-7901 cells in a dose- dependent manner (P < 0.05); the cell apoptotic rate also dramatically increased after curcumin incubation in a dose-dependent manner (P < 0.05). After co-administration with diazoxide, however, we found that both the MMP-loss-inducing and the apoptosis-inducing effects of curcumin in SGC-7901 cells were significantly impaired (all P < 0.05). As a result, the proliferation of SGC-7901 cells was maintained by diazoxide treatment.
  
  
  CONCLUSIONS
  Impaired mitoKATP opening causes MMP loss, and is involved in curcumin-induced apoptosis in gastric cancer.
authors.affiliation: Department of General Surgery, second affiliated hospital of Lanzhou University, Lanzhou, Gansu, 730000, China. onlyhawk@163.com.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Second Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, China. 810927700@qq.com.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of General Surgery, second affiliated hospital of Lanzhou University, Lanzhou, Gansu, 730000, China. renhongdoctor@gmail.com.
authors.forename: Ailin
authors.initials: A
authors.lastname: Song
authors.affiliation: Department of Pathology, Lanzhou University Medical School, 199 West Donggang Road, Lanzhou, Gansu, 730000, China. zhangxiaoyun1981@foxmail.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Lanzhou University Medical School, 199 West Donggang Road, Lanzhou, Gansu, 730000, China. Zhangxu64@163.com.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Lanzhou University, 199 West Donggang Road, Lanzhou, Gansu, 730000, China. hxd@lzu.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: He
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D003981:Diazoxide / Q000494:pharmacology; D005434:Flow Cytometry; D064593:Heterografts; D006801:Humans; D054086:KATP Channels / Q000378:metabolism*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D054086:KATP Channels; D014665:Vasodilator Agents; D003474:Curcumin; D003981:Diazoxide