title: Neutron autoradiography to study boron compound microdistribution in an oral cancer model.
abstract: OBJECTIVE
  We previously reported the therapeutic efficacy of Sequential Boron Neutron Capture Therapy (Seq-BNCT), i.e., BPA (boronophenylalanine) - BNCT followed by GB-10 (decahydrodecaborate) - BNCT 1 or 2 days later, in the hamster cheek pouch oral cancer model. We have utilized the neutron autoradiography methodology to study boron microdistribution in tissue. The aim was to use this method to evaluate if the distribution of GB-10 is altered by prior application of BPA-BNCT in Sequential BNCT protocols.
  
  
  METHODS
  Extensive qualitative and quantitative autoradiography analyses were performed in the following groups: G1 (animals without boron); G2 (animals injected with BPA); G3 (animals injected with GB-10); G4 (same as G3, 24 h after BPA-BNCT); and G5 (same protocol as G4, 48 h interval).
  
  
  RESULTS
  A detailed study of boron localization in the different tissue structures of tumor, premalignant and normal tissue in the hamster cheek pouch was performed. GB-10 accumulated preferentially in non-neoplastic connective tissue, whereas for BPA neoplastic cells showed the highest boron concentration. Boron distribution was less heterogeneous for GB-10 than for BPA. In premalignant and normal tissue, GB-10 and BPA accumulated mostly in connective tissue and epithelium, respectively.
  
  
  CONCLUSIONS
  BPA-BNCT could alter boron microlocalization of GB-10 administered subsequently. Boron targeting homogeneity is essential for therapeutic success.
authors.affiliation: National Atomic Energy Commission (CNEA) , San Martin, Buenos Aires , Argentina.
authors.forename: Agustina
authors.initials: A
authors.lastname: Portu
authors.forename: Ana Julia
authors.initials: AJ
authors.lastname: Molinari
authors.forename: Silvia Inés
authors.initials: SI
authors.lastname: Thorp
authors.forename: Emiliano César Cayetano
authors.initials: EC
authors.lastname: Pozzi
authors.forename: Paula
authors.initials: P
authors.lastname: Curotto
authors.forename: Amanda Elena
authors.initials: AE
authors.lastname: Schwint
authors.forename: Gisela
authors.initials: G
authors.lastname: Saint Martin
mesh_terms: D000818:Animals; D001345:Autoradiography; D001896:Boron Compounds / Q000493:pharmacokinetics*; D016754:Boron Neutron Capture Therapy; D006224:Cricetinae; D006801:Humans; D008647:Mesocricetus; D009062:Mouth Neoplasms / Q000532:radiotherapy*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: C436045:4-dihydroxyborylphenylalanine; D001896:Boron Compounds; D010649:Phenylalanine
keywords: BPA; GB-10; Neutron autoradiography; Sequential BNCT; boron microdistribution; oral cancer