title: The production of multiprotein complexes in insect cells using the baculovirus expression system.
abstract: The production of a homogeneous protein sample in sufficient quantities is an essential prerequisite not only for structural investigations but represents also a rate-limiting step for many functional studies. In the cell, a large fraction of eukaryotic proteins exists as large multicomponent assemblies with many subunits, which act in concert to catalyze specific activities. Many of these complexes cannot be obtained from endogenous source material, so recombinant expression and reconstitution are then required to overcome this bottleneck. This chapter describes current strategies and protocols for the efficient production of multiprotein complexes in large quantities and of high quality, using the baculovirus/insect cell expression system.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/UDS, BP 163, 67404, Illkirch Cedex, France.
authors.forename: Wassim
authors.initials: W
authors.lastname: Abdulrahman
authors.forename: Laura
authors.initials: L
authors.lastname: Radu
authors.forename: Frederic
authors.initials: F
authors.lastname: Garzoni
authors.forename: Olga
authors.initials: O
authors.lastname: Kolesnikova
authors.forename: Kapil
authors.initials: K
authors.lastname: Gupta
authors.forename: Judit
authors.initials: J
authors.lastname: Osz-Papai
authors.forename: Imre
authors.initials: I
authors.lastname: Berger
authors.forename: Arnaud
authors.initials: A
authors.lastname: Poterszman
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D007313:Insecta / Q000166:cytology / Q000235:genetics / Q000821:virology*; D046912:Multiprotein Complexes / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D046912:Multiprotein Complexes; D011994:Recombinant Proteins