title: A robust generic method for grid detection in white light microscopy Malassez blade images in the context of cell counting.
abstract: In biology, cell counting is a primary measurement and it is usually performed manually using hemocytometers such as Malassez blades. This work is tedious and can be automated using image processing. An algorithm based on Fourier transform filtering and the Hough transform was developed for Malassez blade grid extraction. This facilitates cell segmentation and counting within the grid. For the present work, a set of 137 images with high variability was processed. Grids were accurately detected in 98% of these images.
authors.affiliation: 1Service Systèmes d'Information,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Ambroise
authors.initials: A
authors.lastname: Marin
authors.affiliation: 1Service Systèmes d'Information,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Denimal
authors.affiliation: 3UMR A 02.102 Procédés Alimentaires et Microbiologiques,équipe Procédés Microbiologiques et Biotechnologiques,AgroSup Dijon / Université de Bourgogne,1 esplanade Erasme,21000 Dijon,France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Guyot
authors.affiliation: 2Groupe Modélisation Numérique en Agronomie et AgroAlimentaire,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Ludovic
authors.initials: L
authors.lastname: Journaux
authors.affiliation: 2Groupe Modélisation Numérique en Agronomie et AgroAlimentaire,AgroSup Dijon,26 boulevard Docteur Petitjean,21000 Dijon,France.
authors.forename: Paul
authors.initials: P
authors.lastname: Molin
mesh_terms: D000465:Algorithms; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000254:growth & development
keywords: image analysis