title: Natural abundance (δ¹⁵N) indicates shifts in nitrogen relations of woody taxa along a savanna-woodland continental rainfall gradient.
abstract: Water and nitrogen (N) interact to influence soil N cycling and plant N acquisition. We studied indices of soil N availability and acquisition by woody plant taxa with distinct nutritional specialisations along a north Australian rainfall gradient from monsoonal savanna (1,600-1,300 mm annual rainfall) to semi-arid woodland (600-250 mm). Aridity resulted in increased 'openness' of N cycling, indicated by increasing δ(15)N(soil) and nitrate:ammonium ratios, as plant communities transitioned from N to water limitation. In this context, we tested the hypothesis that δ(15)N(root) xylem sap provides a more direct measure of plant N acquisition than δ(15)N(foliage). We found highly variable offsets between δ(15)N(foliage) and δ(15)N(root) xylem sap, both between taxa at a single site (1.3-3.4 ‰) and within taxa across sites (0.8-3.4 ‰). As a result, δ(15)N(foliage) overlapped between N-fixing Acacia and non-fixing Eucalyptus/Corymbia and could not be used to reliably identify biological N fixation (BNF). However, Acacia δ(15)N(root) xylem sap indicated a decline in BNF with aridity corroborated by absence of root nodules and increasing xylem sap nitrate concentrations and consistent with shifting resource limitation. Acacia dominance at arid sites may be attributed to flexibility in N acquisition rather than BNF capacity. δ(15)N(root) xylem sap showed no evidence of shifting N acquisition in non-mycorrhizal Hakea/Grevillea and indicated only minor shifts in Eucalyptus/Corymbia consistent with enrichment of δ(15)N(soil) and/or decreasing mycorrhizal colonisation with aridity. We propose that δ(15)N(root) xylem sap is a more direct indicator of N source than δ(15)N(foliage), with calibration required before it could be applied to quantify BNF.
authors.affiliation: Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, 14853, USA, fms46@cornell.edu.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Soper
authors.forename: Anna E
authors.initials: AE
authors.lastname: Richards
authors.forename: Ilyas
authors.initials: I
authors.lastname: Siddique
authors.forename: Marcos P M
authors.initials: MP
authors.lastname: Aidar
authors.forename: Garry D
authors.initials: GD
authors.lastname: Cook
authors.forename: Lindsay B
authors.initials: LB
authors.lastname: Hutley
authors.forename: Nicole
authors.initials: N
authors.lastname: Robinson
authors.forename: Susanne
authors.initials: S
authors.lastname: Schmidt
mesh_terms: D000045:Acacia / Q000254:growth & development / Q000378:metabolism; D001315:Australia; D055864:Droughts; D017753:Ecosystem; D005052:Eucalyptus / Q000254:growth & development / Q000378:metabolism; D065928:Forests; D065948:Grassland; D038821:Mycorrhizae; D009584:Nitrogen / Q000378:metabolism*; D058458:Nitrogen Cycle; D009586:Nitrogen Fixation; D009587:Nitrogen Isotopes / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D011891:Rain; D012987:Soil; D014197:Trees; D014867:Water; D014934:Wood / Q000378:metabolism; D052584:Xylem / Q000378:metabolism
chemical_list: D009587:Nitrogen Isotopes; D012987:Soil; D014867:Water; D009584:Nitrogen