title: siRNA targeting TCTP suppresses osteosarcoma cell growth and induces apoptosis in vitro and in vivo.
abstract: Osteosarcoma (OS) remains the most frequent primary malignant bone tumor in adolescents. However, the molecular cause of the disease is poorly elucidated. In the present study, we primarily found that translationally controlled tumor protein (TCTP) was overexpressed in human OS tissues and cell lines. To investigate the function of TCTP in OS cell growth, an RNA interference lentivirus system was employed to deplete TCTP expression in Saos-2 and U2OS cell lines. Specific knockdown of TCTP significantly impaired cell proliferation and colony-formation capacity in both OS cell lines. Moreover, depletion of TCTP caused a significant accumulation of OS cells in the S phase and eventually induced cell apoptosis. Expression levels of the G2/M phase regulators cyclin B1 and Cdc25A were decreased, and apoptotic markers Bad and caspase-3 were increased in both OS cell lines after depletion of TCTP. Furthermore, depletion of TCTP potently inhibited the growth of xenografts in nude mice. Our results indicate that inhibition of TCTP expression exerts potential antitumor activity and may be a novel therapeutic approach in human OS.
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Shen
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Cheng-Bo
authors.initials: CB
authors.lastname: Qu
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Hai-Kun
authors.initials: HK
authors.lastname: Chu
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Cui
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Yuan-Xin
authors.initials: YX
authors.lastname: Sun
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Yin-Dong
authors.initials: YD
authors.lastname: Song
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Orthopedic Ward 24, Daqing Oil Field General Hospital, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Shi
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D002453:Cell Cycle; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008819:Mice, Nude; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology / Q000628:therapy*; D034741:RNA, Small Interfering / Q000235:genetics / Q000627:therapeutic use*; D000067492:RNAi Therapeutics; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; C000715647:TPT1 protein, human; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1
keywords: RNA interference; TCTP; apoptosis; osteosarcoma; proliferation