title: Antioxidant and anti-inflammatory activities of porphyran isolated from discolored nori (Porphyra yezoensis).
abstract: We found that discolored waste nori with no commercial value, contains much higher level of porphyran than normal nori that is a sheeted food stuff prepared from P. yezoensis used in sushi. Chemical analyses revealed that mean molecular mass of the porphyran prepared from discolored nori (dc-porphyran) was much lower than that of the porphyran from normal nori (n-porphyran). Dc-porphyran showed slightly greater scavenging activity toward superoxide anion and hydroxyl radical than n-porphyran. Dc-porphyran inhibited nitric oxide (NO) production in LPS-stimulated RAW264.7 cells through preventing the expression of inducible NO synthase, whereas no such activity was observed in n-porphyran. Since acid-hydrolyzed n-porphyran showed the inhibitory activity on NO production from LPS-stimulated RAW264.7 cells, the molecular size of porphyran was suggested to be a critical factor for the activity. Dc-porphyran was separated into 4 fractions (F1-F4) on DEAE-chromatography, and F1 showed the highest inhibitory effect on NO production from LPS-stimulated RAW264.7 cells. Our results indicate that discolored waste nori is useful as a source of porphyran with even better bioactivities than porphyran from normal nori.
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Isaka
authors.affiliation: Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea; Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
authors.forename: Kichul
authors.initials: K
authors.lastname: Cho
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nakazono
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan.
authors.forename: Ryogo
authors.initials: R
authors.lastname: Abu
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan.
authors.forename: Mikinori
authors.initials: M
authors.lastname: Ueno
authors.affiliation: Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea; Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea. Electronic address: dkim@kbsi.re.kr.
authors.forename: Daekyung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan; Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan. Electronic address: t-oda@nagasaki-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Oda
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D044664:Porphyra / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012685:Sepharose / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; C038549:porphyran; D009569:Nitric Oxide; D012685:Sepharose; D052247:Nitric Oxide Synthase Type II
keywords: Anti-inflammatory activity; Antioxidant activity; Porphyra yezoensis