title: Molecular cloning and characterization of the full-length Hsp90 gene from Matricaria recutita.
abstract: Heat shock protein 90 (Hsp90) is one of the most abundant and conserved chaperone proteins and plays important roles in plant growth and responses to environmental stimuli. However, little is known regarding the sequence and function of Hsp90s in Matricaria recutita. In the present study, we cloned the full-length cDNA sequence of the hsp90 gene from this species. Using rapid amplification of cDNA ends technologies with 2 degenerate primers that were designed based on the hsp90 gene sequence from other members of Asteraceae, we isolated and characterized an Hsp90 homolog gene from M. recutita (Mr-Hsp90). The full-length Mr-hsp90 cDNA sequence, containing 2097 base pairs, encodes a protein of 698 amino acids. Based on amino acid sequence identity, Mr-Hsp90 showed high similarity to other cloned Hsp90 proteins. The Mr-Hsp90 protein was closely clustered with the Lactuca sativa in a phylogenetic tree. These results indicate that the cloned sequence of Mr-Hsp90 is a member of the Hsp90 family, which is reported for the first time in M. recutita. Next, we conducted a salt stress experiment to determine the protein's function under salt stress conditions. Survival of chamomile seedlings subjected to heat-shock pretreatment was significantly increased compared with groups that had not undergone heat-shock pretreatment in a salt stress environment. This indicates that Mr-Hsp90 plays an important role in the salt resistance of chamomile seedlings.
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: S P
authors.initials: SP
authors.lastname: Ling
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: S S
authors.initials: SS
authors.lastname: Su
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: H M
authors.initials: HM
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: X S
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China.
authors.forename: G F
authors.initials: GF
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Anhui Agricultural University, Hefei China zhiwuxue239@163.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D019143:Evolution, Molecular; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response; D031168:Matricaria / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054712:Salinity; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D018841:HSP90 Heat-Shock Proteins; D010940:Plant Proteins