title: A new strategy to construct a FRET platform for ratiometric sensing of hydrogen sulfide.
abstract: We introduce a new FRET strategy to construct a ratiometric fluorescent H2S sensor. The ratio emission signal of the coumarin-naphthalimide dyad is modulated by the FRET process, which works in coordination with the ICT mechanism. The FRET process on/off is controlled through tuning the overlap level of the donor emission spectrum with the acceptor absorption via modulation of the acceptor fluorophore absorption wavelength. was applied to visualize both the intracellular exogenous and endogenous H2S through blue and green emission channels.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, China.
authors.forename: Longwei
authors.initials: L
authors.lastname: He
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
authors.forename: Qiuyan
authors.initials: Q
authors.lastname: Xu
authors.forename: Haipeng
authors.initials: H
authors.lastname: Wei
mesh_terms: D003374:Coumarins / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006862:Hydrogen Sulfide / Q000032:analysis* / Q000737:chemistry; D053644:Naphthalimides / Q000737:chemistry*
chemical_list: D003374:Coumarins; D053644:Naphthalimides; C030123:coumarin; D006862:Hydrogen Sulfide