title: Distribution of 8 periodontal microorganisms in family members of Chinese patients with aggressive periodontitis.
abstract: OBJECTIVE
  To date, no information on the distribution of periodontal microorganisms among family members of Chinese patients with aggressive peridontitis (AgP) is available. The aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members.
  
  
  METHODS
  Saliva and pooled subgingival plaque samples were collected from 103 participants from 41 nuclear families (including 41 AgP probands, 19 mothers, 22 fathers, 21 siblings). Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis-positive individuals by PCR.
  
  
  RESULTS
  P. gingivalis, T. forsythia, T. denticola, C. rectus and F. nucleatum were the most frequently detected species both in AgP probands and in their relatives. Kappa statistical analysis revealed that the detection of A. actinomycetemcomitans (Kappa = 0.503) and F. nucleatum (Kappa = 0.565) in probands was highly consistent with that in their relatives. Most probands shared the identical fimA genotype of P. gingivalis with their relatives.
  
  
  CONCLUSIONS
  Our results suggested that the intrafamilial transmission of periodontal microorganisms may occur between Chinese patients with aggressive periodontitis and their relatives.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Periodontology and Oral Medicine, ChangSha Stomatological Hospital, No.844, Wuyi Ave, Changsha 410005, Hunan, PR China.
authors.forename: Lilei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China. Electronic address: xulihome@263.net.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Huanxin
authors.initials: H
authors.lastname: Meng
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Periodontics, Department and Hospital of Stomatology, Shanxi Medical University, Xinjian Nanlu 63#, Taiyuan 030001, Shanxi province, PR China.
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Dong
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, 22 Zhongguancun Nandajie, Haidian District, Beijing 100081, PR China.
authors.forename: Xiane
authors.initials: X
authors.lastname: Wang
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D010520:Aggressive Periodontitis / Q000382:microbiology*; D041963:Bacteroidetes / Q000302:isolation & purification; D044883:Campylobacter rectus / Q000302:isolation & purification; D002681:China; D003773:Dental Plaque / Q000382:microbiology; D004587:Electrophoresis, Agar Gel; D005260:Female; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008297:Male; D009679:Nuclear Family; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D018713:Prevotella intermedia / Q000302:isolation & purification; D045242:Prevotella nigrescens / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology; D048373:Treponema denticola / Q000302:isolation & purification
keywords: Aggressive periodontitis; Genotype; Microbiology; Saliva