title: Ins1(Cre) knock-in mice for beta cell-specific gene recombination.
abstract: OBJECTIVE
  Pancreatic beta cells play a central role in the control of glucose homeostasis by secreting insulin to stimulate glucose uptake by peripheral tissues. Understanding the molecular mechanisms that control beta cell function and plasticity has critical implications for the pathophysiology and therapy of major forms of diabetes. Selective gene inactivation in pancreatic beta cells, using the Cre-lox system, is a powerful approach to assess the role of particular genes in beta cells and their impact on whole body glucose homeostasis. Several Cre recombinase (Cre) deleter mice have been established to allow inactivation of genes in beta cells, but many show non-specific recombination in other cell types, often in the brain.
  
  
  METHODS
  We describe the generation of Ins1(Cre) and Ins1(CreERT2) mice in which the Cre or Cre-oestrogen receptor fusion protein (CreERT2) recombinases have been introduced at the initiation codon of the Ins1 gene.
  
  
  RESULTS
  We show that Ins1(Cre) mice induce efficient and selective recombination of floxed genes in beta cells from the time of birth, with no recombination in the central nervous system. These mice have normal body weight and glucose homeostasis. Furthermore, we show that tamoxifen treatment of adult Ins1(CreERT2) mice crossed with Rosa26-tdTomato mice induces efficient recombination in beta cells.
  
  
  CONCLUSIONS
  These two strains of deleter mice are useful new resources to investigate the molecular physiology of pancreatic beta cells.
authors.affiliation: Center for Integrative Genomics, University of Lausanne, Genopode Building, CH-1015, Lausanne, Switzerland, Bernard.Thorens@unil.ch.
authors.forename: Bernard
authors.initials: B
authors.lastname: Thorens
authors.forename: David
authors.initials: D
authors.lastname: Tarussio
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Maestro
authors.forename: Meritxell
authors.initials: M
authors.lastname: Rovira
authors.forename: Eija
authors.initials: E
authors.lastname: Heikkilä
authors.forename: Jorge
authors.initials: J
authors.lastname: Ferrer
mesh_terms: D000818:Animals; D005260:Female; D005951:Glucose Tolerance Test; D007328:Insulin / Q000235:genetics / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains
chemical_list: C514817:Ins1 protein, mouse; D007328:Insulin; C045073:Cre recombinase; D019426:Integrases