title: The endothelial permeability increased by low voltage and high frequency electroporation.
abstract: Propose: Standard electroporation and electrochemotherapy caused the endothelial cell permeability and reduction in tumor blood flow. The effects of low voltage and high frequency electroporation on the endothelial cells permeability and viability were expected. Therefore, the propose of present study was to evaluate the effect of electroporation with bleomycin or alone on viability and permeabilization of human Embryo microvascular endothelial (HUVEC) cell line.
  
  
  METHODS
  The HUVEC cells were exposed to 4000 electric pulsed with 100µs duration, 50-150 v/cm with increment of 10 v/cm in 4, 5 and 6 kHz frequency (33 experimental groups) and then uptake and viability reduction was measured in each group.
  
  
  RESULTS
  The results of this study demonstrated that electric pulses alone reduce the cell viability and with bleomycin significantly increases the toxicity to endothelial cells.
  
  
  CONCLUSIONS
  Our data indicated that low voltage, high frequency ECT is highly cytotoxic for a HUVEC cell offers a possible effect of antivascular actions of this kind of electrochemotherapy.
authors.affiliation: Department of Medical Physics, faculty of medical science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Shankayi
authors.affiliation: Department of Medical Physics, faculty of medical science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Pourmirjafari Firoozabadi
authors.affiliation: Department of Immunology, faculty of medical science, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Zohair Saraf
keywords: Electrochemotherapy; Electroporation; Endothelia cell; High frequency; low voltage