title: Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae.
abstract: Benz[a]anthracene (BaA) is a ubiquitous polycyclic aromatic hydrocarbon found in numerous aquatic ecosystems. However, ecotoxicological data in aquatic organisms are scarce. To remedy this lack of data, Japanese medaka (Oryzias latipes) embryos were exposed to BaA and toxic effects were investigated at multiple toxicological endpoints. Japanese medaka embryos were incubated onto BaA-spiked artificial sediment for 9 days at low or moderate environmental concentrations ranging from 0.9 to 12 µgg(-1) dw. BaA-exposed embryos exhibited significant tachycardia. BaA exposure was also shown to increase CYP1A activity in the hepato-biliary tissue as well as craniofacial deformities and DNA damage in pro-larvae. The photomotor response of BaA-exposed larvae was reduced in comparison to the control group. According to this set of tests, the lowest tested and observed effect concentration (LOEC) for Japanese medaka early life stages was equivalent to 0.92 µgg(-1) dw of BaA. This concentration fall into the range of concentrations frequently encountered in sediments of polluted aquatic ecosystems. Taking into consideration these results, BaA represents a threat for fish early life stages in particular those developing onto or into contaminated sediments.
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Florane
authors.initials: F
authors.lastname: Le Bihanic
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Vivien
authors.initials: V
authors.lastname: Sommard
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: de Lansalut
authors.initials: de L
authors.lastname: Perrine
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Anaïk
authors.initials: A
authors.lastname: Pichon
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Grasset
authors.affiliation: Atoxigen Laboratory, 33600 Pessac, France.
authors.forename: Saadia
authors.initials: S
authors.lastname: Berrada
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Hélène
authors.initials: H
authors.lastname: Budzinski
authors.affiliation: IFREMER, Ecotoxicology Laboratory, Place Gaby Coll, BP7, 17137 L'Houmeau, France; INRA LPGP, Campus de Beaulieu, 35042 Rennes, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Cousin
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Morin
authors.affiliation: University of Bordeaux, EPOC, UMR CNRS 5805, 33405 Talence, France. Electronic address: jerome.cachot@u-bordeaux.fr.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Cachot
mesh_terms: D000818:Animals; D000873:Anthracenes; D001551:Benz(a)Anthracenes / Q000633:toxicity*; D004249:DNA Damage; D004777:Environment; D005399:Fishes / Q000235:genetics; D007814:Larva / Q000187:drug effects / Q000235:genetics; D008027:Light; D015162:Micronucleus Tests; D009043:Motor Activity / Q000187:drug effects*; D009990:Oryzias / Q000235:genetics; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000873:Anthracenes; D001551:Benz(a)Anthracenes; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; C030935:benz(a)anthracene; C034020:anthracene
keywords: Benz[a]anthracene; DNA damage; In vivo EROD activity; Spiked sediment; Swimming behavior; Teratogenicity