title: Anti-diabetic effect mediated by Ramulus mori polysaccharides.
abstract: Diabetes mellitus is a complicated metabolic disease, whose pathogenesis is related to apoptosis within pancreatic tissue. In this study, the potential therapeutic benefits of Ramulus mori polysaccharides (RMP) on streptozotocin (STZ)-induced diabetic mice were evaluated. Our experiments indicated that RMP lowered hyperglycemia and increased insulin levels in diabetic mice. Histopathological examination revealed that RMP contributed to the reduction of STZ-lesioned pancreatic cells. In addition, the serum level of HbA1c was decreased. RMP treatment also showed increased Bcl-2 expression and reduced Bax protein level in pancreatic tissue. Furthermore, intrapancreatic expressions of p-JNK, p-p38 and cleaved-caspase-3 were down-regulated by RMP treatment. Collectively, the findings demonstrate that RMP exerts the pronounced hypoglycemic effect via regulation of the intrapancreatic JNK/p38 pathway to protect against STZ-induced apoptosis in pancreatic tissue, eventually ameliorating metabolic function in the pancreas.
authors.affiliation: Department of Pharmacy, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi 533000, PR China.
authors.forename: Lingyuan
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory Breeding Base of Natural Anti-infective Drug Research, YouJiang Medical University for Nationalities, Baise, Guangxi 533000, PR China.
authors.forename: Fenglian
authors.initials: F
authors.lastname: Yang
authors.affiliation: Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi 533000, PR China.
authors.forename: Junli
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Affiliated Hospital of YouJiang Medical University for Nationalities, Baise, Guangxi 533000, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Key Laboratory Breeding Base of Natural Anti-infective Drug Research, YouJiang Medical University for Nationalities, Baise, Guangxi 533000, PR China. Electronic address: huang_baise@126.com.
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D005215:Fasting / Q000097:blood; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008297:Male; D051379:Mice; D031625:Morus / Q000737:chemistry*; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010750:Phosphoproteins / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use; D059748:Proteolysis / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D053148:Caspase 3; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D007328:Insulin; D048031:JNK Mitogen-Activated Protein Kinases; D010750:Phosphoproteins; D011134:Polysaccharides; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Apoptosis; Hyperglycemia; Metabolism; Ramulus mori polysaccharides