title: The mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) and related phylogenetic analyses.
abstract: The complete mitochondrial genome of Dastarcus helophoroides (Coleoptera: Bothrideridae) which consists of 13 PCGs, 22 tRNA genes, two rRNA genes and a non-coding region (D-loop), is sequenced for its nucleotide sequence of 15,878 bp (GenBank: KF811054.1). The genome has a typical gene order which is identical to other Coleoptera species. Except for COI gene generally starts with non-canonical initial codon, all protein-coding genes start with ATN codon and terminate with the stop codon TA(A) or TAG. The secondary structure of rrnL and rrnS consists of 48 helices (contains four newly proposed helices) and 35 helices (contains two newly proposed helices) respectively. All 22 tRNAs in D. helophoroides are predicted to fold into typical cloverleaf secondary structure, except trnS1 (AGN), in which the dihydrouracil arm (DHU arm) could not form stable stem-loop structure. Thirteen protein-coding genes (nucleotide dataset and nucleic acid dataset) of the available species (29 taxa) have been used to infer the phylogenetic relationships among these orders. Tenebrionoidea and Cucujoidea form a sister group, and D. helophoroides is classified into Cucujoidea (Bothrideridae). The study first research on the phylogenetic analyses involving to the D. helophoroides mitogenome, and the results strongly bolster the current morphology-based hypothesis.
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Zhengqing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Forestry Technology Promotion Center of Xian, Xian, Shaanxi 710061, China.
authors.forename: Xiaoji
authors.initials: X
authors.lastname: Wang
authors.affiliation: Forestry Technology Promotion Center of Xian, Xian, Shaanxi 710061, China.
authors.forename: Rongzhou
authors.initials: R
authors.lastname: Li
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Ruijian
authors.initials: R
authors.lastname: Guo
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Song
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Hao
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China.
authors.forename: Huapeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Forestry Pests Biological Control, College of Forestry, Northwest Agriculture and Forestry University, Yangling, Shaanxi 712100, China. Electronic address: limenglou@hotmail.com.
authors.forename: Menglou
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001517:Coleoptera / Q000235:genetics*; D054629:Genome, Mitochondrial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D022661:RNA, Untranslated / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012335:RNA, Ribosomal; D022661:RNA, Untranslated; D012343:RNA, Transfer
keywords: Dastarcus helophoroides; Mitochondrial genome; Molecular phylogeny; Sequence analysis