title: Endoplasmic reticulum stress inhibition is a valid therapeutic strategy in vitrifying oocytes.
abstract: The aim of this study is to determine the link between oocyte cryopreservation and endoplasmic reticulum (ER) stress; whether ER stress inhibition improves the efficiency of oocyte vitrification is also explored. Oocytes from mice were exposure to tauroursodeoxycholic acid (TUDCA, an ER stress inhibitor) or TM (tunicamycin, an ER stress inducer) with or without vitrification. The expressions of X-box binding protein-1 (XBP-1) protein and caspase-12 protein, viability of vitrified-warmed oocytes, and their subsequent embryo competence were measured. The levels of XBP-1 protein and caspase-12 protein expression in vitrified-warmed oocytes were significantly higher than those of fresh control oocytes. TUDCA improved the viability of vitrified-warmed oocytes and their subsequent embryo competence. Mouse oocyte cryopreservation is associated with ER stress, and ER stress inhibition improves the efficiency of oocyte vitrification.
authors.affiliation: Department of Andrology, People's Hospital of Liaocheng City, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, People's Hospital of Linyi City, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Liu
authors.affiliation: Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Li
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ya-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Ru-Xin
authors.initials: RX
authors.lastname: Chen
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Wei
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Reproductive Medicine, Hospital for Maternity and Child Care of Jinan City, China. Electronic address: jmzjnfy@163.com.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D053454:Caspase 12 / Q000096:biosynthesis; D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000502:physiology*; D000071701:Regulatory Factor X Transcription Factors; D013655:Taurochenodeoxycholic Acid / Q000494:pharmacology*; D014157:Transcription Factors / Q000096:biosynthesis; D014415:Tunicamycin / Q000494:pharmacology*; D058989:Vitrification; D000071717:X-Box Binding Protein 1
chemical_list: D004268:DNA-Binding Proteins; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D000071717:X-Box Binding Protein 1; C000605544:Xbp1 protein, mouse; D014415:Tunicamycin; D013655:Taurochenodeoxycholic Acid; C031655:ursodoxicoltaurine; C506440:Casp12 protein, mouse; D053454:Caspase 12
keywords: Caspase-12; Endoplasmic reticulum stress; Oocytes; Vitrification