title: Constitutively active ESCRT-II suppresses the MVB-sorting phenotype of ESCRT-0 and ESCRT-I mutants.
abstract: The endosomal sorting complex required for transport (ESCRT) protein complexes function at the endosome in the formation of intraluminal vesicles (ILVs) containing cargo proteins destined for the vacuolar/lysosomal lumen. The early ESCRTs (ESCRT-0 and -I) are likely involved in cargo sorting, whereas ESCRT-III and Vps4 function to sever the neck of the forming ILVs. ESCRT-II links these functions by initiating ESCRT-III formation in an ESCRT-I-regulated manner. We identify a constitutively active mutant of ESCRT-II that partially suppresses the phenotype of an ESCRT-I or ESCRT-0 deletion strain, suggesting that these early ESCRTs are not essential and have redundant functions. However, the ESCRT-III/Vps4 system alone is not sufficient for ILV formation but requires cargo sorting mediated by one of the early ESCRTs.
authors.affiliation: Center for Cell and Genome Science and Department of Biology, University of Utah, Salt Lake City, UT 84112.
authors.forename: Shrawan Kumar
authors.initials: SK
authors.lastname: Mageswaran
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309.
authors.forename: Natalie K
authors.initials: NK
authors.lastname: Johnson
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309 babst@biology.utah.edu odorizzi@colorado.edu.
authors.forename: Greg
authors.initials: G
authors.lastname: Odorizzi
authors.affiliation: Center for Cell and Genome Science and Department of Biology, University of Utah, Salt Lake City, UT 84112 babst@biology.utah.edu odorizzi@colorado.edu.
authors.forename: Markus
authors.initials: M
authors.lastname: Babst
mesh_terms: D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000502:physiology*; D057146:Multivesicular Bodies / Q000502:physiology*; D050505:Mutant Proteins / Q000378:metabolism; D021381:Protein Transport / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000502:physiology
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport; D050505:Mutant Proteins; D029701:Saccharomyces cerevisiae Proteins