title: Functional neuropeptidomics in invertebrates.
abstract: Neuropeptides are key messengers in almost all physiological processes. They originate from larger precursors and are extensively processed to become bioactive. Neuropeptidomics aims to comprehensively identify the collection of neuropeptides in an organism, organ, tissue or cell. The neuropeptidome of several invertebrates is thoroughly explored since they are important model organisms (and models for human diseases), disease vectors and pest species. The charting of the neuropeptidome is the first step towards understanding peptidergic signaling. This review will first discuss the latest developments in exploring the neuropeptidome. The physiological roles and modes of action of neuropeptides can be explored in two ways, which are largely orthogonal and therefore complementary. The first way consists of inferring the functions of neuropeptides by a forward approach where neuropeptide profiles are compared under different physiological conditions. Second is the reverse approach were neuropeptide collections are used to screen for receptor-binding. This is followed by localization studies and functional tests. This review will focus on how these different functional screening methods contributed to the field of invertebrate neuropeptidomics and expanded our knowledge of peptidergic signaling. This article is part of a Special Issue entitled: Neuroproteomics: Applications in Neuroscience and Neurology.
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium.
authors.forename: Wouter
authors.initials: W
authors.lastname: De Haes
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium.
authors.forename: Elien
authors.initials: E
authors.lastname: Van Sinay
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium.
authors.forename: Giel
authors.initials: G
authors.lastname: Detienne
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium.
authors.forename: Liesbet
authors.initials: L
authors.lastname: Temmerman
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium.
authors.forename: Liliane
authors.initials: L
authors.lastname: Schoofs
authors.affiliation: Functional Genomics and Proteomics, Department of Biology, University of Leuven (KU Leuven), Naamsestraat 59, 3000 Leuven, Belgium. Electronic address: Kurt.Boonen@bio.kuleuven.be.
authors.forename: Kurt
authors.initials: K
authors.lastname: Boonen
mesh_terms: D000818:Animals; D006801:Humans; D007448:Invertebrates / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
chemical_list: D009479:Neuropeptides
keywords: Endocrinology; GPCR; Mass spectrometry; Neuropeptide; Peptidomics