title: B-Myb regulates snail expression to promote epithelial-to-mesenchymal transition and invasion of breast cancer cell.
abstract: Breast cancer is the leading cause of cancer death in women worldwide, which is closely related to metastasis. Recent studies argue that breast cancer cells that have undergone epithelial-to-mesenchymal transition (EMT) acquire aggressive malignant properties, but the molecular mechanisms underlying this transition are poorly understood. In this study, we found that siRNA-mediated attenuation of B-Myb expression restored E-cadherin expression and cell-cell junction formation in breast cancer cells, suppressing cell invasion, anchorage-independent growth, and tumor formation. In contrast, the forced B-Myb expression decreased the expression of the epithelial marker E-cadherin, but increased the mesenchymal markers in breast cancer cells. We found that B-Myb upregulated expression of the key EMT regulator snail and that it mediated EMT activation and cell invasion by B-Myb.
authors.affiliation: Department of Oncological Surgery, Luqiao Hospital of Taizhou Enze Medical Group, Luqiao, Zhejiang, China.
authors.forename: Deyou
authors.initials: D
authors.lastname: Tao
authors.forename: Yihong
authors.initials: Y
authors.lastname: Pan
authors.forename: Guobin
authors.initials: G
authors.lastname: Jiang
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Lu
authors.forename: Song
authors.initials: S
authors.lastname: Zheng
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
authors.forename: Feilin
authors.initials: F
authors.lastname: Cao
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D005260:Female; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D009361:Neoplasm Invasiveness / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000071250:Snail Family Transcription Factors; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis*; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; C064352:MYBL2 protein, human; D000071250:Snail Family Transcription Factors; D015534:Trans-Activators; D014157:Transcription Factors