title: The current incidence of viral disease in korean sweet potatoes and development of multiplex rt-PCR assays for simultaneous detection of eight sweet potato viruses.
abstract: Sweet potato is grown extensively from tropical to temperate regions and is an important food crop worldwide. In this study, we established detection methods for 17 major sweet potato viruses using single and multiplex RT-PCR assays. To investigate the current incidence of viral diseases, we collected 154 samples of various sweet potato cultivars showing virus-like symptoms from 40 fields in 10 Korean regions, and analyzed them by RT-PCR using specific primers for each of the 17 viruses. Of the 17 possible viruses, we detected eight in our samples. Sweet potato feathery mottle virus (SPFMV) and sweet potato virus C (SPVC) were most commonly detected, infecting approximately 87% and 85% of samples, respectively. Furthermore, Sweet potato symptomless virus 1 (SPSMV-1), Sweet potato virus G (SPVG), Sweet potato leaf curl virus (SPLCV), Sweet potato virus 2 ( SPV2), Sweet potato chlorotic fleck virus (SPCFV), and Sweet potato latent virus (SPLV) were detected in 67%, 58%, 47%, 41%, 31%, and 20% of samples, respectively. This study presents the first documented occurrence of four viruses (SPVC, SPV2, SPCFV, and SPSMV-1) in Korea. Based on the results of our survey, we developed multiplex RT-PCR assays for simple and simultaneous detection of the eight sweet potato viruses we recorded.
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Hae-Ryun
authors.initials: HR
authors.lastname: Kwak
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Jun-Chul
authors.initials: JC
authors.lastname: Shin
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Ye-Ji
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.affiliation: Bioenergy Crop Research Center, National Institute of Crop Science, Muan 534-833, Korea.
authors.forename: Hyeong-Un
authors.initials: HU
authors.lastname: Lee
authors.affiliation: Bioenergy Crop Research Center, National Institute of Crop Science, Muan 534-833, Korea.
authors.forename: Mi-Nam
authors.initials: MN
authors.lastname: Jung
authors.affiliation: Department of Environmental Horticulture, University of Seoul, Seoul 130-743, Korea.
authors.forename: Sun-Hyung
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Crop Protection Division, National Academy of Agricultural Science, Wanju 565-851, Korea.
authors.forename: Hong-Soo
authors.initials: HS
authors.lastname: Choi
keywords: disease incidence; multiplex RT-PCR; sweet potato viruses