title: In vitro and in vivo modifications of recombinant and human IgG antibodies.
abstract: Tremendous knowledge has been gained in the understanding of various modifications of IgG antibodies, driven mainly by the fact that antibodies are one of the most important groups of therapeutic molecules and because of the development of advanced analytical techniques. Recombinant monoclonal antibody (mAb) therapeutics expressed in mammalian cell lines and endogenous IgG molecules secreted by B cells in the human body share some modifications, but each have some unique modifications. Modifications that are common to recombinant mAb and endogenous IgG molecules are considered to pose a lower risk of immunogenicity. On the other hand, modifications that are unique to recombinant mAbs could potentially pose higher risk. The focus of this review is the comparison of frequently observed modifications of recombinant monoclonal antibodies to those of endogenous IgG molecules.
authors.affiliation: a Protein Characterization; Alexion Pharmaceuticals Inc .; Cheshire , CT USA.
authors.forename: Hongcheng
authors.initials: H
authors.lastname: Liu
authors.forename: Gomathinayagam
authors.initials: G
authors.lastname: Ponniah
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Zhang
authors.forename: Christine
authors.initials: C
authors.lastname: Nowak
authors.forename: Alyssa
authors.initials: A
authors.lastname: Neill
authors.forename: Nidia
authors.initials: N
authors.lastname: Gonzalez-Lopez
authors.forename: Rekha
authors.initials: R
authors.lastname: Patel
authors.forename: Guilong
authors.initials: G
authors.lastname: Cheng
authors.forename: Adriana Z
authors.initials: AZ
authors.lastname: Kita
authors.forename: Bruce
authors.initials: B
authors.lastname: Andrien
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D006031:Glycosylation; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D021382:Protein Sorting Signals / Q000235:genetics; D011761:Pyrrolidonecarboxylic Acid / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D011761:Pyrrolidonecarboxylic Acid
keywords: C-terminal lysine; endogenous IgG antibodies; leader sequence; oligosaccharides; posttranslational modifications; pyroglutamate; recombinant monoclonal antibodies