title: Sulfate resupply accentuates protein synthesis in coordination with nitrogen metabolism in sulfur deprived Brassica napus.
abstract: To investigate the regulatory interactions between S assimilation and N metabolism in Brassica napus, de novo synthesis of amino acids and proteins was quantified by (15)N and (34)S tracing, and the responses of transporter genes, assimilatory enzymes and metabolites pool involving in nitrate and sulfate metabolism were assessed under continuous sulfur supply, sulfur deprivation and sulfate resupply after 3 days of sulfur (S) deprivation. S-deprived plants were characterized by a strong induction of sulfate transporter genes, ATP sulfurylase (ATPS) and adenosine 5'-phosphosulfate reductase (APR), and by a repressed activity of nitrate reductase (NR) and glutamine synthetase (GS). Sulfate resupply to the S-deprived plants strongly increased cysteine, amino acids and proteins concentration. The increase in sulfate and cysteine concentration caused by sulfate resupply was not matched with the expression of sulfate transporters and the activity of ATPS and APR which were rapidly decreased by sulfate resupply. A strong induction of O-acetylserine(thiol)lyase (OASTL), NR and GS upon sulfate resupply was accompanied with the increase in cysteine, amino acids and proteins pool. Sulfate resupply resulted in a strong increase in de novo synthesis of amino acids and proteins, as evidenced by the increases in N and S incorporation into amino acids (1.8- and 2.4-fold increase) and proteins (2.2-and 6.3-fold increase) when compared to S-deprived plants. The results thus indicate that sulfate resupply followed by S-deprivation accelerates nitrate assimilation for protein synthesis.
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju 500-600, Republic of Korea.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju 500-600, Republic of Korea; Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Bok-Rye
authors.initials: BR
authors.lastname: Lee
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju 500-600, Republic of Korea.
authors.forename: Sang-Hyun
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju 500-600, Republic of Korea.
authors.forename: Rashed
authors.initials: R
authors.lastname: Zaman
authors.affiliation: Université de Caen Basse-Normandie, Esplanade de la Paix, F-14032 Caen Cedex, France; UCBN, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, 14032 Caen Cedex, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Avice
authors.affiliation: Université de Caen Basse-Normandie, Esplanade de la Paix, F-14032 Caen Cedex, France; UCBN, UMR INRA-UCBN 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, 14032 Caen Cedex, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Ourry
authors.affiliation: Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Buk-Gwangju P.O Box 205, Gwangju 500-600, Republic of Korea. Electronic address: grassl@chonnam.ac.kr.
authors.forename: Tae-Hwan
authors.initials: TH
authors.lastname: Kim
mesh_terms: D029688:Brassica napus / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis*; D014176:Protein Biosynthesis / Q000187:drug effects*; D013431:Sulfates / Q000494:pharmacology*; D013455:Sulfur / Q000172:deficiency
chemical_list: D010940:Plant Proteins; D013431:Sulfates; D013455:Sulfur; D009584:Nitrogen
keywords: (15)N and (34)S chase; Brassica napus; Interaction N and S; S deprivation; Sulfate assimilation