title: Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation.
abstract: Thermoresponsive, cationic, copolymer brushes poly(N-isopropylacrylamide(IPAAm)-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide(tBAAm)) and poly(IPAAm-co-3-acrylamidopropyl trimethylammonium chloride-co-tBAAm) were prepared on glass substrates through surface-initiated atom transfer radical polymerization. Prepared copolymer brushes were investigated as thermally modulated cell separation materials. Densely packed cationic copolymer brushes were formed on the glass substrates, and the positive charge density was modulated by controlling the composition of cationic moieties and species. During observation of cell adhesion and detachment properties on copolymer brushes, human bone marrow mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell adhesion and detachment, while other bone-marrow-derived cells did not adhere. Using these properties, hbmMSC could be purified from mixtures of human bone-marrow-derived cells simply by changing the external temperature. Therefore, the prepared cationic copolymer brush is useful for separation of hbmMSC.
authors.affiliation: Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns) , 8-1 Kawadacho, Shinjuku, Tokyo 162-8666, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Nagase
authors.forename: Yuri
authors.initials: Y
authors.lastname: Hatakeyama
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Matsuura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Naoya
authors.initials: N
authors.lastname: Takeda
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013696:Temperature
chemical_list: D011108:Polymers