title: Gene expression and protein secretion during human mesenchymal cell differentiation into adipogenic cells.
abstract: BACKGROUND
  Mesenchymal stromal cells (MSC) can be obtained from potentially any tissue from the human body, but cells purified from different sources are undoubtedly different, and for each medical application, the MSC with the best regenerative potential should be chosen.
  
  
  RESULTS
  Bone marrow-derived mesenchymal stromal cells (BM-MSC), adipose tissue-derived mesenchymal stromal cells (AT-MSC) and Wharton's Jelly-derived mesenchymal stromal cells (WJ-MSC) were isolated from human tissues and were cultured under differentiation media supplemented with fetal bovine serum. We quantified the expression of stem cell and adipocyte genetic markers using quantitative real time PCR, as well as the secretion of cytokines, extracellular matrix components and growth factors using Luminex and ELISA. All three MSC differentiated into adipogenic cells. AT-MSC showed the highest shift in ADIPOQ, CEBPA and PPARG mRNA expression. BM-MSC kept high expression levels of stem-cell markers SOX2 and POU5F1. WJ-MSC showed the lowest increase in mRNA expression when cells were induced to differentiate into adipocytes. Regarding protein secretion, adipocyte-like cells generated from WJ-MSC secreted the highest chemokine levels. AT-MSC-derived adipocyte-like cells secreted the lowest cytokine amounts and the highest quantity of collagen types I and III. Adipocyte-like cells obtained from BM-MSC secreted high amounts of most angiogenic factors, growth factors TGF-β1 and TGF-β2, collagens type II and IV, heparan sulfate, laminin and aggrecan.
  
  
  CONCLUSIONS
  Mesenchymal stromal cells purified from different tissues have a different behavior when induced to differentiate into adipocyte-like cells.
authors.affiliation: Excellion Biomedical Services S.A., Rua Afrânio de Mello Franco 333, Quitandinha, Petrópolis, Rio de Janeiro, Brazil. pramable@gmail.com.
authors.forename: Paola Romina
authors.initials: PR
authors.lastname: Amable
authors.affiliation: National Institute of Metrology, Quality and Technology (Inmetro), Xerém, Rio de Janeiro, Brazil. mtellesteixeira@gmail.com.
authors.forename: Marcus Vinicius Telles
authors.initials: MV
authors.lastname: Teixeira
authors.affiliation: National Institute of Metrology, Quality and Technology (Inmetro), Xerém, Rio de Janeiro, Brazil. rosanabizon@gmail.com.
authors.forename: Rosana Bizon Vieira
authors.initials: RB
authors.lastname: Carias
authors.affiliation: National Institute of Metrology, Quality and Technology (Inmetro), Xerém, Rio de Janeiro, Brazil. jmgranjeiro@inmetro.gov.br.
authors.forename: José Mauro
authors.initials: JM
authors.lastname: Granjeiro
authors.affiliation: National Institute of Metrology, Quality and Technology (Inmetro), Xerém, Rio de Janeiro, Brazil. rrborojevic@gmail.com.|Faculdade de Medicina de Petrópolis, Faculdades Arthur Sá Earp Neto, Petrópolis, Rio de Janeiro, Brazil. rrborojevic@gmail.com.
authors.forename: Radovan
authors.initials: R
authors.lastname: Borojevic
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D015415:Biomarkers; D001854:Bone Marrow Cells / Q000166:cytology; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D059631:Wharton Jelly / Q000166:cytology
chemical_list: D015415:Biomarkers; D016207:Cytokines; D011506:Proteins