title: The SMART model: Soft Membranes Adapt and Respond, also Transiently, in the presence of antimicrobial peptides.
abstract: Biophysical and structural studies of peptide-lipid interactions, peptide topology and dynamics have changed our view on how antimicrobial peptides insert and interact with membranes. Clearly, both the peptides and the lipids are highly dynamic, change and mutually adapt their conformation, membrane penetration and detailed morphology on a local and a global level. As a consequence, the peptides and lipids can form a wide variety of supramolecular assemblies in which the more hydrophobic sequences preferentially, but not exclusively, adopt transmembrane alignments and have the potential to form oligomeric structures similar to those suggested by the transmembrane helical bundle model. In contrast, charged amphipathic sequences tend to stay intercalated at the membrane interface where they cause pronounced disruptions of the phospholipid fatty acyl packing. At increasing local or global concentrations, the peptides result in transient membrane openings, rupture and ultimately lysis. Depending on peptide-to-lipid ratio, lipid composition and environmental factors (temperature, buffer composition, ionic strength, etc.), the same peptide sequence can result in a variety of those responses. Therefore, the SMART model has been introduced to cover the full range of possibilities. With such a view in mind, novel antimicrobial compounds have been designed from amphipathic polymers, peptide mimetics, combinations of ultra-short polypeptides with hydrophobic anchors or small designer molecules.
authors.affiliation: Université de Strasbourg/CNRS, UMR7177, Institut de Chimie, 4, rue Blaise Pascal, 67070, Strasbourg, France.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Bechinger
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D055592:Biophysical Phenomena; D002462:Cell Membrane / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation
chemical_list: D023181:Antimicrobial Cationic Peptides; D008563:Membrane Lipids
keywords: PGLa; alamethicin; carpet model; cecropin; equlibria; hydrophobic mismatch; local disorder; magainin; membrane macroscopic phase; membrane pore; membrane topology; peptaibol; peptide-lipid interactions; toroidal pore