title: Antitumour efficacy of Piper tuberculatum and piplartine based on the hollow fiber assay.
abstract: Piper tuberculatum, popularly known in Brazil as "jaborandi falso" and "pimenta darta", is widely used in folk medicine for the treatment of several diseases. In this study, the in vivo hollow fiber assay was used to investigate the antitumour efficacy of the crude extract and piplartine obtained from P. tuberculatum roots. Human glioblastoma (SF-295) and colon carcinoma (HCT-8) cell lines were used. In vitro cytotoxicity was assayed by the MTT assay. In the hollow fiber assay, nude mice implanted with tumour cells in hollow fibers were treated for four consecutive days via the intraperitoneal route, and tumour cell populations were assessed by the MTT assay. Both the crude extract and piplartine displayed cytotoxicity. In the hollow fiber assay, tumour growth inhibition rates were 24.6-54.8 % for the crude extract and 33.7-62.2 % for piplartine. No signal of toxicity was noticed. In conclusion, the crude extract and piplartine obtained from P. tuberculatum roots displayed in vitro and in vivo anticancer efficacy.
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil.
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Bezerra
authors.affiliation: Departamento de Biofísica e Fisiologia/Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina, Piauí, Brazil.
authors.forename: Paulo Michel P
authors.initials: PM
authors.lastname: Ferreira
authors.affiliation: Departamento de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Camila Maria L
authors.initials: CM
authors.lastname: Machado
authors.affiliation: Departamento de Química Orgânica, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Nayara C
authors.initials: NC
authors.lastname: de Aquino
authors.affiliation: Departamento de Química Orgânica, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Edilberto R
authors.initials: ER
authors.lastname: Silveira
authors.affiliation: Departamento de Radiologia, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Roger
authors.initials: R
authors.lastname: Chammas
authors.affiliation: Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Pessoa
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D020128:Inhibitory Concentration 50; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D031701:Piper / Q000737:chemistry*; D010881:Piperidones / Q000008:administration & dosage / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D011145:Polyvinyls / Q000008:administration & dosage; D005465:Fluorocarbon Polymers
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010881:Piperidones; D010936:Plant Extracts; D011145:Polyvinyls; C498077:piperlongumine; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers