title: Endometriosis may be associated with mitochondrial dysfunction in cumulus cells from subjects undergoing in vitro fertilization-intracytoplasmic sperm injection, as reflected by decreased adenosine triphosphate production.
abstract: OBJECTIVE
  To determine whether endometriosis is associated with mitochondrial dysfunction in cumulus (granulosa [GC]) cells of subjects undergoing IVF-intracytoplasmic sperm injection (ICSI).
  
  
  METHODS
  Prospective cohort study.
  
  
  METHODS
  An IVF clinic in a tertiary academic care center.
  
  
  METHODS
  Eleven women with endometriosis and 39 controls.
  
  
  METHODS
  None.
  
  
  METHODS
  Cumulus cell adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA), and genomic DNA (gDNA) number.
  
  
  RESULTS
  Cumulus cell ATP content was 65% lower in subjects with surgically proven endometriosis (median 312.5 attomoles/ng total DNA, interquartile range = 116.0-667.8) compared with controls (median 892.4 attomoles/ng total DNA, interquartile range = 403.0-1,412.2). There was no significant difference in mtDNA:gDNA ratio. There were no significant differences in age, body mass index (BMI), basal serum FSH level, total oocyte number, metaphase II (M2) oocyte number, metaphase I oocyte number, percentage of M2 oocytes, fertilization rate, implantation rate, or pregnancy rate (PR). Multivariate regression analysis showed significant positive correlations between ATP and [1] M2 oocyte number (r = 0.307) and [2] pregnancy (r = 0.332). There were also trends toward positive correlations between ATP and [3] age (r = 0.283), [4] total number of oocytes (r = 0.271), [5] percentage of M2 oocytes (r = 0.249), and [6] implantation rate (r = 0.293). There were no statistically significant correlations between mtDNA:gDNA ratio and any demographic factors or clinical outcomes measured.
  
  
  CONCLUSIONS
  Surgically confirmed endometriosis may be associated with cumulus cell mitochondrial dysfunction in subjects undergoing IVF-ICSI for infertility, as reflected by decreased ATP production.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Jones Institute for Reproductive Medicine, Norfolk, Virginia; Division of Reproductive Endocrinology and Infertility, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire. Electronic address: albert.l.hsu@hitchcock.org.
authors.forename: Albert L
authors.initials: AL
authors.lastname: Hsu
authors.affiliation: Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia.
authors.forename: Paige M
authors.initials: PM
authors.lastname: Townsend
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Jones Institute for Reproductive Medicine, Norfolk, Virginia.
authors.forename: Sergio
authors.initials: S
authors.lastname: Oehninger
authors.affiliation: Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, Virginia.
authors.forename: Frank J
authors.initials: FJ
authors.lastname: Castora
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000328:Adult; D015331:Cohort Studies; D054885:Cumulus Cells / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism; D004715:Endometriosis / Q000175:diagnosis / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D011247:Pregnancy; D018873:Pregnancy Rate / Q000639:trends; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial; D000255:Adenosine Triphosphate
keywords: Endometriosis; cumulus cells; infertility; mitochondria