title: Mössbauer spectroscopy of Fe/S proteins.
abstract: Iron-sulfur (Fe/S) clusters are structurally and functionally diverse cofactors that are found in all domains of life. (57)Fe Mössbauer spectroscopy is a technique that provides information about the chemical nature of all chemically distinct Fe species contained in a sample, such as Fe oxidation and spin state, nuclearity of a cluster with more than one metal ion, electron spin ground state of the cluster, and delocalization properties in mixed-valent clusters. Moreover, the technique allows for quantitation of all Fe species, when it is used in conjunction with electron paramagnetic resonance (EPR) spectroscopy and analytical methods. (57)Fe-Mössbauer spectroscopy played a pivotal role in unraveling the electronic structures of the "well-established" [2Fe-2S](2+/+), [3Fe-4S](1+/0), and [4Fe-4S](3+/2+/1+/0) clusters and -more-recently- was used to characterize novel Fe/S clustsers, including the [4Fe-3S] cluster of the O2-tolerant hydrogenase from Aquifex aeolicus and the 3Fe-cluster intermediate observed during the reaction of lipoyl synthase, a member of the radical SAM enzyme superfamily.
authors.affiliation: Department of Chemistry, The Pennsylvania State University, University Park, PA, USA 16802. Electronic address: mxp65@psu.edu.
authors.forename: Maria-Eirini
authors.initials: ME
authors.lastname: Pandelia
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA 16802.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Lanz
authors.affiliation: Department of Chemistry, The Pennsylvania State University, University Park, PA, USA 16802; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA 16802.
authors.forename: Squire J
authors.initials: SJ
authors.lastname: Booker
authors.affiliation: Department of Chemistry, The Pennsylvania State University, University Park, PA, USA 16802; Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA 16802. Electronic address: ckrebs@psu.edu.
authors.forename: Carsten
authors.initials: C
authors.lastname: Krebs
mesh_terms: D000465:Algorithms; D007501:Iron / Q000737:chemistry* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D010084:Oxidation-Reduction; D011487:Protein Conformation; D015204:Spectroscopy, Mossbauer / Q000379:methods*; D013455:Sulfur / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007506:Iron-Sulfur Proteins; D013455:Sulfur; D007501:Iron
keywords: EPR spectroscopy; Fe/S clusters; Mossbauer spectroscopy