title: Lactoferrin induced neuronal differentiation: A boon for brain tumours.
abstract: The cumulative treatments of bovine lactoferrin (bLf) and iron saturated lactoferrin (Fe-bLf) in the neuroblastoma cells showed neuronal differentiating actions evident with the expression of specific differentiating markers, β-tubulin III and neurofilaments. The protein treatments also showed lowered endogenous survivin that is responsible for cell proliferation and the miRNA 584 and miRNA214-3p, required for differentiation. Further, bLf adopted the PI3K signalling predominantly, while Fe-bLf involved both the PI3K and ERK signalling for inducing differentiation. In conclusion, this is the first study to report the neuronal differentiating effects of milk proteins and future studies are warranted for clinical application.
authors.affiliation: Nanomedicine Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Faculty of Health, Deakin University, Geelong Technology Precinct, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Bhasker
authors.initials: B
authors.lastname: Sriramoju
authors.affiliation: Nanomedicine Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Faculty of Health, Deakin University, Geelong Technology Precinct, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia.
authors.forename: Rupinder K
authors.initials: RK
authors.lastname: Kanwar
authors.affiliation: Nanomedicine Laboratory of Immunology and Molecular Biomedical Research (NLIMBR), School of Medicine, Faculty of Health, Deakin University, Geelong Technology Precinct, Pigdons Road, Waurn Ponds, Geelong, Victoria 3217, Australia. Electronic address: jagat.kanwar@deakin.edu.au.
authors.forename: Jagat R
authors.initials: JR
authors.lastname: Kanwar
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D007382:Intermediate Filaments / Q000378:metabolism; D007781:Lactoferrin / Q000494:pharmacology*; D035683:MicroRNAs / Q000378:metabolism; D009447:Neuroblastoma / Q000473:pathology; D009474:Neurons / Q000187:drug effects*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D000077022:Survivin; D014162:Transfection; D014404:Tubulin / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D035683:MicroRNAs; D000077022:Survivin; D014404:Tubulin; D019869:Phosphatidylinositol 3-Kinases; D007781:Lactoferrin
keywords: Bovine lactoferrin; Iron saturated bovine lactoferrin; Neurological disorders; P-glycoprotein; Protein therapeutics