title: Influence of peripheral blood microparticles of pregnant women with preeclampsia on the phenotype of monocytes.
abstract: Platelet- and endothelial-derived microparticles influence the phenotype of peripheral blood leukocytes and induce production of proinflammatory cytokines. The influence of blood plasma microparticles of pregnant women on the surface receptor expression on intact or activated monocytes is still unexplored. This study was carried out to test the hypothesis that peripheral blood microparticles of women with normal pregnancy and women with preeclampsia have different influence on the expression of surface molecules on monocytes. The objective of the study was to evaluate the influence of blood plasma microparticles of pregnant women on the phenotypic properties of intact and activated THP-1 monocytes. Microparticles were isolated from peripheral blood samples of nonpregnant women, healthy pregnant women, and women with preeclampsia. THP-1 cell line was used as a model of monocytes. Microparticles of nonpregnant women decreased CD18, CD49d, and CD54 expressions and increased CD11c, CD31, CD47, and vascular endothelial growth factor receptor 2 expressions. Microparticles of healthy pregnant women increased CD18, CD54, and integrin β7 expressions and decreased CD11a and CD29 expressions. Microparticles of women with preeclampsia decreased CD18 expression on tumor necrosis factor α (TNF-α)-activated ТНР-1 cells. Microparticles of nonpregnant women, women with normal pregnancy, and pregnant women with preeclampsia decreased CD181 expression on intact and TNF-α-activated THP-1 cells. Therefore, blood plasma microparticles of women with normal pregnancy and women with preeclampsia have different influences on the expression of surface molecules on THP-1 monocytes.
authors.affiliation: Department of Immunology, D.O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Dmitriy I
authors.initials: DI
authors.lastname: Sokolov
authors.affiliation: Department of Immunology, D.O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Olga M
authors.initials: OM
authors.lastname: Ovchinnikova
authors.affiliation: Department of Immunology, D.O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Daniil A
authors.initials: DA
authors.lastname: Korenkov
authors.affiliation: Department of Immunology, D.O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia.
authors.forename: Alice N
authors.initials: AN
authors.lastname: Viknyanschuk
authors.affiliation: Department of biology, Saint Petersburg State University, Saint Petersburg, Russia.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Benken
authors.affiliation: Department of biology, Saint Petersburg State University, Saint Petersburg, Russia.
authors.forename: Kirril V
authors.initials: KV
authors.lastname: Onokhin
authors.affiliation: Department of Immunology, D.O. Ott Research Institute of Obstetrics and Gynecology, Saint Petersburg, Russia. Electronic address: selkovsa@mail.ru.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Selkov
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D002460:Cell Line; D055252:Cell-Derived Microparticles / Q000648:ultrastructure; D005260:Female; D006801:Humans; D018625:Microscopy, Atomic Force; D009000:Monocytes / Q000502:physiology*; D011225:Pre-Eclampsia / Q000097:blood*; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D014409:Tumor Necrosis Factor-alpha; C000588866:KDR protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1; D040301:Vascular Endothelial Growth Factor Receptor-2