title: Cortical fast-spiking parvalbumin interneurons enwrapped in the perineuronal net express the metallopeptidases Adamts8, Adamts15 and Neprilysin.
abstract: The in situ hybridization Allen Mouse Brain Atlas was mined for proteases expressed in the somatosensory cerebral cortex. Among the 480 genes coding for protease/peptidases, only four were found enriched in cortical interneurons: Reln coding for reelin; Adamts8 and Adamts15 belonging to the class of metzincin proteases involved in reshaping the perineuronal net (PNN) and Mme encoding for Neprilysin, the enzyme degrading amyloid β-peptides. The pattern of expression of metalloproteases (MPs) was analyzed by single-cell reverse transcriptase multiplex PCR after patch clamp and was compared with the expression of 10 canonical interneurons markers and 12 additional genes from the Allen Atlas. Clustering of these genes by K-means algorithm displays five distinct clusters. Among these five clusters, two fast-spiking interneuron clusters expressing the calcium-binding protein Pvalb were identified, one co-expressing Pvalb with Sst (PV-Sst) and another co-expressing Pvalb with three metallopeptidases Adamts8, Adamts15 and Mme (PV-MP). By using Wisteria floribunda agglutinin, a specific marker for PNN, PV-MP interneurons were found surrounded by PNN, whereas the ones expressing Sst, PV-Sst, were not.
authors.affiliation: Centre de Psychiatrie et Neurosciences, Institut National de la Santé et de la Recherche Médicale CPN INSERM U894, Université Paris Descartes, Hôpital Sainte Anne, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Rossier
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: A
authors.initials: A
authors.lastname: Bernard
authors.affiliation: Department of Psychiatry, Center for Psychiatric Neuroscience, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Prilly-Lausanne, Switzerland.
authors.forename: J-H
authors.initials: JH
authors.lastname: Cabungcal
authors.affiliation: Ecole Supérieure de Physique et de Chimie Industrielles UMR 7637 CNRS-ESPCI, Paris, France.
authors.forename: Q
authors.initials: Q
authors.lastname: Perrenoud
authors.affiliation: Centre de Psychiatrie et Neurosciences, Institut National de la Santé et de la Recherche Médicale CPN INSERM U894, Université Paris Descartes, Hôpital Sainte Anne, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Savoye
authors.affiliation: Ecole Supérieure de Physique et de Chimie Industrielles UMR 7637 CNRS-ESPCI, Paris, France.
authors.forename: T
authors.initials: T
authors.lastname: Gallopin
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: M
authors.initials: M
authors.lastname: Hawrylycz
authors.affiliation: Department of Psychiatry, Center for Psychiatric Neuroscience, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Prilly-Lausanne, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Cuénod
authors.affiliation: Department of Psychiatry, Center for Psychiatric Neuroscience, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Prilly-Lausanne, Switzerland.
authors.forename: K
authors.initials: K
authors.lastname: Do
authors.affiliation: Centre de Psychiatrie et Neurosciences, Institut National de la Santé et de la Recherche Médicale CPN INSERM U894, Université Paris Descartes, Hôpital Sainte Anne, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Urban
authors.affiliation: Allen Institute for Brain Science, Seattle, WA, USA.
authors.forename: Ed S
authors.initials: ES
authors.lastname: Lein
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000378:metabolism*; D000071096:ADAMTS Proteins; D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D016000:Cluster Analysis; D066298:In Vitro Techniques; D007395:Interneurons / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D015260:Neprilysin / Q000235:genetics / Q000378:metabolism*; D010320:Parvalbumins / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D037121:Plant Lectins / Q000378:metabolism; D034781:Receptors, N-Acetylglucosamine / Q000378:metabolism; D000091042:Reelin Protein; D066191:Sensorimotor Cortex / Q000166:cytology*
chemical_list: D010320:Parvalbumins; D037121:Plant Lectins; D034781:Receptors, N-Acetylglucosamine; D000091042:Reelin Protein; C040129:wisteria lectin; C000717128:Reln protein, mouse; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C000601877:ADAMTS15 protein, mouse; C498472:Adamts8 protein, mouse; D015260:Neprilysin