title: Protection effect of atorvastatin in cerebral ischemia-reperfusion injury rats by blocking the mitochondrial permeability transition pore.
abstract: The aim of this study was to investigate the influence of atorvastatin on the opening of the mitochondrial permeability transition pore (MPTP) and the expression of cytochrome C (Cyt C) in Sprague-Dawley rats with cerebral ischemia-reperfusion (I/R). The rat model of cerebral artery ischemia was established by the suture-occluded method with ischemia for 2 h and reperfusion for 72 h. Thirty-four male rats were randomly divided into four groups: the normal group and the sham-operation group without any treatment, the I/R group with only intragastric administration of normal saline, and the intervention group, which received intragastric administration of 10 mg/kg atorvastatin at different times. All rats were sacrificed at 72 h. Compared with the I/R group, the morphology of nerve cells in the intervention group was reduced, the number of TUNEL-positive cells decreased, the expression of cortical cytoplasm Cyt C decreased, and the mitochondrial absorbance value increased. All of these differences were statistically significant. Atorvastatin could inhibit neuronal apoptosis and alleviate the cerebral I/R injury. The mechanism may be related to the blocking of the MPTP opening and the subsequent reduction of Cyt C release.
authors.affiliation: Department of Neurological Rehabilitation, Mawangdui Hospital of Hunan Province, Hunan Provincial Research Institute of Geriatrics, Changsha, China.
authors.forename: T
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Neurological Rehabilitation, Mawangdui Hospital of Hunan Province, Hunan Provincial Research Institute of Geriatrics, Changsha, China.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Neurological Rehabilitation, Mawangdui Hospital of Hunan Province, Hunan Provincial Research Institute of Geriatrics, Changsha, China xitaocn@163.com.
authors.forename: X
authors.initials: X
authors.lastname: Tao
authors.affiliation: Department of Neurological Rehabilitation, Mawangdui Hospital of Hunan Province, Hunan Provincial Research Institute of Geriatrics, Changsha, China.
authors.forename: J G
authors.initials: JG
authors.lastname: Deng
mesh_terms: D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D000069059:Atorvastatin; D002545:Brain Ischemia / Q000378:metabolism / Q000517:prevention & control*; D045304:Cytochromes c / Q000378:metabolism; D004195:Disease Models, Animal; D006538:Heptanoic Acids / Q000494:pharmacology*; D020244:Infarction, Middle Cerebral Artery / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects*; D000083162:Mitochondrial Permeability Transition Pore; D011758:Pyrroles / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D000924:Anticholesteremic Agents; D006538:Heptanoic Acids; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D011758:Pyrroles; D045304:Cytochromes c; D000069059:Atorvastatin