title: Growth-modulatory effects of heparin and VEGF165 on the choriocarcinoma cell-line JEG-3 and its expression of heparanase.
abstract: BACKGROUND
  Expression of heparanase (HPSE) in tumor cells is strongly associated with invasion, metastasis and angiogenesis. It also plays a key role during pregnancy, in processes of implantation as well as placentation. Vascular endothelial growth factor (VEGF) and heparin are known to alter HPSE expression, with heparin given prophylactically to women with a history of placenta-mediated complications in subsequent pregnancies.
  
  
  METHODS
  We examined the growth-modulatory effects of different concentrations of heparin and VEGF on the choriocarcinoma cell-line JEG-3 and the expression of heparanase under VEGF and heparin by proliferation assays, PCR, and western blot.
  
  
  RESULTS
  Proliferation of JEG-3 cells was induced by heparin in a dose-dependent manner, whereas highly concentrated VEGF led to a decreased cell proliferation. Both agents did not influence the HPSE-expression.
  
  
  CONCLUSIONS
  The presumed pregnancy-protecting effects of heparin may partially be due to an increase of trophoblast proliferation and not via regulation of HPSE expression.
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany michael.bohlmann@uksh.de.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Bohlmann
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
authors.forename: Jana-Christin
authors.initials: JC
authors.lastname: Hoerster
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
authors.forename: Diana
authors.initials: D
authors.lastname: Bundschuh
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Boemicke
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital Oldenburg, Oldenburg, Germany.
authors.forename: Doerte W
authors.initials: DW
authors.lastname: Luedders
authors.affiliation: Department of Obstetrics and Gynaecology, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Köster
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002822:Choriocarcinoma / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis; D014327:Trophoblasts / Q000201:enzymology / Q000473:pathology; D014594:Uterine Neoplasms / Q000201:enzymology* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology*; D000099175:Heparanase
chemical_list: D005966:Glucuronidase; D006493:Heparin; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D000099175:Heparanase; C467484:VEGFA protein, human
keywords: Heparin; PCR; VEGF; choriocarcinoma; heparanase; proliferation