title: Remediation of pharmaceuticals and personal care products using an aerobic granular sludge sequencing bioreactor and microbial community profiling using Solexa sequencing technology analysis.
abstract: Recently, a new type of organic pollution derived from pharmaceuticals and personal care products (PPCPs) is gradually on the rise. Wastewater treatment to remove PPCPs was investigated using an aerobic granular sludge sequencing bioreactor (GSBR). After optimization of influent organic load, hydraulic shear stress, sludge settling time, etc., aerobic granular sludge was analyzed for its physiological and biochemical characteristics and tested for its efficacy to remove PPCPs wastewater. The granular sludge effectively removed some but not all of the PPCPs tested; removal correlated with the microbial profiles in the granules, as assessed using Solexa sequencing technology. Sequencing revealed the presence of five phylogenetic groups: Proteobacteria, Bacteroidetes, Betaproteobacteria, an unclassified genus, and Zoogloea. The results demonstrated changes in the microbial profiles with time in response to the presence of PPCPs. The effects of PPCPs on microbial communities in granular sludge process are discussed.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; College of Petrochemical Technology, Lanzhou University of Technology, Lanzhou 730050, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Jinchunzi
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal & Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: shenjimin@hit.edu.cn.
authors.forename: Jimin
authors.initials: J
authors.lastname: Shen
authors.affiliation: College of Petrochemical Technology, Lanzhou University of Technology, Lanzhou 730050, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
mesh_terms: D000332:Aerobiosis; D001419:Bacteria / Q000145:classification*; D001673:Biodegradation, Environmental; D044822:Biodiversity; D018533:Biomass; D001704:Biopolymers / Q000032:analysis; D019149:Bioreactors / Q000382:microbiology*; D005110:Extracellular Space / Q000737:chemistry; D006795:Household Products; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA / Q000379:methods*; D012722:Sewage / Q000382:microbiology*
chemical_list: D001704:Biopolymers; D004364:Pharmaceutical Preparations; D012722:Sewage
keywords: Degradation; Granular sludge sequencing bioreactor (GSBR); Microbial community; Pharmaceuticals and personal care products (PPCPs); Solexa sequencing