title: EPR-based approach for the localization of paramagnetic metal ions in biomolecules.
abstract: Metal ions play an important role in the catalysis and folding of proteins and oligonucleotides. Their localization within the three-dimensional fold of such biomolecules is therefore an important goal in understanding structure-function relationships. A trilateration approach for the localization of metal ions by means of long-range distance measurements based on electron paramagnetic resonance (EPR) is introduced. The approach is tested on the Cu(2+) center of azurin, and factors affecting the precision of the method are discussed.
authors.affiliation: Institute of Physical and Theoretical Chemistry, University of Bonn, Wegelerstrasse 12, 53115 Bonn (Germany) http://www.schiemann.uni-bonn.de.
authors.forename: Dinar
authors.initials: D
authors.lastname: Abdullin
authors.forename: Nicole
authors.initials: N
authors.lastname: Florin
authors.forename: Gregor
authors.initials: G
authors.lastname: Hagelueken
authors.forename: Olav
authors.initials: O
authors.lastname: Schiemann
mesh_terms: D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D008280:Magnetics; D008670:Metals / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*
chemical_list: D008670:Metals; D011506:Proteins
keywords: EPR spectroscopy; binding sites; copper; spin labels; triangulation