title: Genetic diversity of Sogatella furcifera (Hemiptera: Delphacidae) in China detected by inter-simple sequence repeats.
abstract: The white-backed planthopper (WBPH), Sogatella furcifera (Horváth) is a serious pest causing grievous damage to rice plants. In the present study, inter-simple sequence repeats were employed to investigate the genetic diversity of 108 samples from 27 WBPH geographic populations in China. Ten primers were screened out with 147 amplified bands, average percentage of polymorphic bands, polymorphic information content, and marker index were 78.9, 0.456, and 6.753% respectively. The results indicated that genetic diversity was different among populations, but genetic variation was as low as 0.2% among the populations and as high as 99.8% within the same geographic population. Among the examined WBPH populations, genetic distances were weakly correlated to geographic distance, and there was no correlation between genetic identity and elevation. Cluster analysis showed that the 27 WBPH populations studied could be lumped into four clusters, with which the results of principal coordinate analysis (were almost consistent. In conclusion, the molecular genetic data demonstrated that the region consisting of Yunnan, Guizhou, Guangdong, and Guangxi was the first landing area of WBPH in its migrating process from overwintering sites to China.
authors.affiliation: The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China.
authors.forename: Jia-Nan
authors.initials: JN
authors.lastname: Xie
authors.affiliation: The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Guo
authors.affiliation: The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China daochaojin@126.com.
authors.forename: Dao-Chao
authors.initials: DC
authors.lastname: Jin
authors.affiliation: The Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang, Guizhou 550025, China.
authors.forename: Xue-Jian
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D000531:Altitude; D025041:Animal Migration; D000818:Animals; D001483:Base Sequence; D002681:China; D016000:Cluster Analysis; D004247:DNA / Q000235:genetics; D014644:Genetic Variation; D005843:Geography; D006430:Hemiptera / Q000235:genetics*; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
chemical_list: D004247:DNA
keywords: ISSR-PCR; genetic diversity; geographic population; white-backed planthopper