title: Biophysical characterization of lectin-glycan interactions for therapeutics, vaccines and targeted drug-delivery.
abstract: Lectin-glycan interactions play a role in biological processes, host-pathogen interactions and in disease. A more detailed understanding of these interactions is not only useful for the elucidation of their biological function but can also be applied in immunology, drug development and delivery and diagnostics. We review some commonly used biophysical techniques for studying lectin-glycan interactions; namely: frontal affinity chromatography, glycan/lectin microarray, surface plasmon resonance, electrochemical impedance spectroscopy, isothermal titration calorimetry, fluorescent assays, enzyme linked lectin sorbent assay and saturation transfer difference nuclear magnetic resonance spectroscopy. Each method is evaluated on efficiency, cost and throughput. We also consider the advantages and limitations of each technique and provide examples of their application in biology, drug discovery and delivery, immunology, glycoprofiling and biosensing.
authors.affiliation: School of Chemistry & Molecular Biosciences, The University of Queensland, St. Lucia, QLD 4072, Australia.
authors.forename: Michelle P
authors.initials: MP
authors.lastname: Christie
authors.forename: Istvan
authors.initials: I
authors.lastname: Toth
authors.forename: Pavla
authors.initials: P
authors.lastname: Simerská
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis; D011485:Protein Binding; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D004337:Drug Carriers; D037102:Lectins; D011134:Polysaccharides; D014614:Vaccines, Synthetic