title: In vitro antioxidant activity and in vivo antifatigue effect of layered double hydroxide nanoparticles as delivery vehicles for folic acid.
abstract: Folic acid antioxidants were successfully intercalated into layered double hydroxides (LDH) nanoparticles according to a previous method with minor modification. The resultant folic acid-LDH constructs were then characterized by X-ray powder diffraction and transmission electron microscopy. The in vitro antioxidant activities, cytotoxicity effect, and in vivo antifatigue were examined by a series of assays. The results showed that folic acid-LDH antioxidant system can scavenge 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals and chelate pro-oxidative Cu(2+). The in vitro cytotoxicity assays indicated that folic acid-LDH antioxidant system had no significant cytotoxic effect or obvious toxicity to normal cells. It also prolonged the forced swimming time of the mice by 32% and 51% compared to folic acid and control groups, respectively. It had an obvious effect on decreasing the blood urea nitrogen and blood lactic acid, while increasing muscle and hepatic glycogen levels. Therefore, folic acid-LDH might be used as a novel antioxidant and antifatigue nutritional supplement.
authors.affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Life Science and Technology, Tongji University, Shanghai, People's Republic of China.
authors.forename: Wenrui
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.
authors.forename: Songhui
authors.initials: S
authors.lastname: You
authors.affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.
authors.forename: Jingmei
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Physical Education, Tongji University, Shanghai, People's Republic of China.
authors.forename: Jibing
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001522:Behavior, Animal / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry / Q000633:toxicity; D005221:Fatigue; D005492:Folic Acid / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006878:Hydroxides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D008297:Male; D051379:Mice
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D005609:Free Radicals; D006878:Hydroxides; D005492:Folic Acid
keywords: antioxidant supplementation; biomaterials; drug delivery; free radicals