title: Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein.
abstract: BACKGROUND
  To identify the chemical structure of Coreopsis tinctoria extracts and their effect and mechanism on reducing blood lipid in hyperlipemia mice.
  
  
  METHODS
  The flavonoids were extracted from Coreopsis tinctoria. The chemical structure was identified by HPLC. 59 mice were divided randomly into 5 groups. (group 1: normal diet control; group 2: hyperlipemia model; group 3: hyperlipemia mice treated with Coreopsis tinctoria, low dose 100 mg/kg; group 4: hyperlipemia mice treated with Coreopsis tinctoria high dose group 200 mg/kg; group 5 hyperlipemia mice treated with Fenofibrate. After 2 week of hyperlipid diet, the treatment of Coreopsis tinctoria and Fenofibrate were given for another 6 weeks with continuous hyperlipid diet. The TC, TG, HDL, histology, adipose differentiation-related protein (ADRP) expression in different groups were compared.
  
  
  RESULTS
  Compared with normal diet group, TC, TG in hyperlipemia model group increased ( P<0.01). After treatment with Coreopsis tinctoria low dose group, high dose group, TC of the hyperlipemia mice decreased (P<0.05) without increasing AST, ALT and ALP. Fenofibrate can also decrease TC and TG but increase AST, ALT and ALP. Expression of hepatic ADRP increased in hyperlipemia mice. Coreopsis tinctoria high dose group 200 mg/kg can inhibit ADRP as Fenofibrate does.
  
  
  CONCLUSIONS
  The flavonoids from Coreopsis tinctoria extracts can reduce blood lipid without liver function damage, showing better anti- hyperlipemia effect than Fenofibrate by down-regulating ADRP.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.forename: Xinmei
authors.initials: X
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Xue
authors.forename: Jiangyun
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Department of Internal Medicine, The First Affiliated Hospital of Xinjiang Medical University Urumqi, Xinjiang 830000, China. xinhua_chen@zju.edu.cn.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
authors.forename: Muhuyati
authors.initials: M
authors.lastname: Wulasihan
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D031945:Coreopsis / Q000737:chemistry*; D015536:Down-Regulation; D004435:Eating; D005419:Flavonoids / Q000627:therapeutic use*; D006949:Hyperlipidemias / Q000188:drug therapy*; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000201:enzymology / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D009929:Organ Size; D000070780:Perilipin-2; D014280:Triglycerides / Q000378:metabolism
chemical_list: D005419:Flavonoids; D008565:Membrane Proteins; D000070780:Perilipin-2; C000602983:Plin2 protein, mouse; D014280:Triglycerides; D002784:Cholesterol