title: CXCL10/XCL1 fusokine elicits in vitro and in vivo chemotaxis.
abstract: Fusokines are proteins formed by the fusion of two cytokines. They have greater bioavailability and therapeutic potential than individual cytokines or a combination of different cytokines. Interferon-gamma-inducible protein 10 (CXCL10) and lymphotactin (XCL1) are members of the chemotactic family of cytokines, which induce tumor regression by eliciting immune-system cell chemotaxis. We engineered a replication-deficient adenoviral system expressing CXCL10/XCL1 fusokine (Ad FIL) and assessed its chemotactic response in vitro and in vivo. The CXCL10/XCL1 fusokine elicited a greater chemotactic effect in IL-2 stimulated lymphocytes than individual or combined cytokines in vitro. CXCL10/XCL1 fusokine biological activity was demonstrated in vivo by intratumoral chemoattraction of CXCR3+ cells. Thus, this novel CXCL10/XCL1 fusokine may represent a potential tool for gene therapy treatment of cancer and other illnesses that require triggering immune-system cell recruitment.
authors.affiliation: Departamento de Histologia, Facultad de Medicina, Universidad Autonoma de Nuevo Leon (UANL), Madero y Aguirre Pequeño s/n Mitras Centro, CP: 64665, Monterrey, NL, Mexico, yesanlug@gmail.com.
authors.forename: Yessica E
authors.initials: YE
authors.lastname: Sanchez-Lugo
authors.forename: Jose J
authors.initials: JJ
authors.lastname: Perez-Trujillo
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Gutierrez-Puente
authors.forename: Aracely
authors.initials: A
authors.lastname: Garcia-Garcia
authors.forename: Humberto
authors.initials: H
authors.lastname: Rodriguez-Rocha
authors.forename: Oralia
authors.initials: O
authors.lastname: Barboza-Quintana
authors.forename: Gerardo E
authors.initials: GE
authors.lastname: Muñoz-Maldonado
authors.forename: Odila
authors.initials: O
authors.lastname: Saucedo-Cardenas
authors.forename: Roberto Montes
authors.initials: RM
authors.lastname: de Oca-Luna
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Loera-Arias
mesh_terms: D000818:Animals; D002460:Cell Line; D054357:Chemokine CXCL10 / Q000235:genetics / Q000378:metabolism*; D019744:Chemokines, C / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis; D005822:Genetic Vectors; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D017921:Mastadenovirus / Q000235:genetics*; D008810:Mice, Inbred C57BL
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D019744:Chemokines, C; C092390:XCL1 protein, human