title: [Chemical constituents of Kadsura oblongifolia and evaluation of their toxicity].
abstract: To study the chemical constituents of K. oblongifolia, silica gel column chromatography, MCI and Sephadex LH-20 were used to separate the 70% acetone extract of the stems of K. oblongifolia. The structures of the isolated compounds have been established on the basis of physicochemical and NMR spectroscopic evidence as well as ESI-MS in some cases. Twenty compounds were obtained and identified as heteroclitalignan A (1), kadsulignan F (2), kadoblongifolin C (3), schizanrin F (4), heteroclitalignan C (5), kadsurarin (6), kadsulignan O (7), eburicol (8), meso-dihydroguaiaretic acid (9), kadsufolin A (10), tiegusanin M (11), heteroclitin B (12), (7'S)-parabenzlactone (13), angeloylbinankadsurin B (14), propinquain H (15), quercetin (16), kadsulignan P (17), schizanrin G (18), micrandilactone C (19) and (-)-shikimic acid (20). Compouds 1, 5, 8, 11-15, 18 and 20 were isolated from this plant for the first time. Toxicity of compounds 1-10 were evaluated with zebrafish model to observe the effect on its embryonic development and heart function. The results showed that compounds 7, 9 and 10 caused edema of zebrafish embryo and decreased the heart rate of zebrafish, which exhibited interference effect on heart development of zebrafish.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Wang
authors.forename: Ke-Chun
authors.initials: KC
authors.lastname: Liu
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: He
authors.forename: Yao-Dong
authors.initials: YD
authors.lastname: Qi
authors.forename: Ben-Gang
authors.initials: BG
authors.lastname: Zhang
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Liu
authors.forename: Pei-Gen
authors.initials: PG
authors.lastname: Xiao
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects; D006139:Guaiacol / Q000031:analogs & derivatives / Q000633:toxicity; D032135:Kadsura / Q000737:chemistry*; D017705:Lignans / Q000633:toxicity; D010936:Plant Extracts / Q000633:toxicity*; D011794:Quercetin / Q000633:toxicity; D014315:Triterpenes / Q000633:toxicity; D015027:Zebrafish / Q000196:embryology
chemical_list: D017705:Lignans; D010936:Plant Extracts; D014315:Triterpenes; C542721:kadoblongifolin C; C568426:kadsulignan O; C568427:kadsulignan P; C517928:micrandilactone C; C015816:dihydroguaiaretic acid; D006139:Guaiacol; D011794:Quercetin