title: Patchouli alcohol protects against lipopolysaccharide-induced acute lung injury in mice.
abstract: BACKGROUND
  Patchouli alcohol (PA), a natural compound isolated from Pogostemon cablin, has been reported to possess anti-inflammatory activity. However, the effects of PA on lipopolysaccharide (LPS)-induced acute lung injury (ALI) have not yet been studied. In the present study, we investigated in vivo the effect of PA on ALI induced by LPS.
  
  
  METHODS
  Mice were administrated intranasally with LPS to induce lung injury. PA was administrated intraperitoneally 1 h before or after the LPS challenge.
  
  
  RESULTS
  The results showed that PA significantly decreased the wet-to-dry weight ratio of lungs and the number of total cells, neutrophils, and macrophages in bronchoalveolar lavage fluid at 7 h after the LPS challenge. In addition, PA also suppressed the production of inflammatory cytokines, such as tumor necrosis factor-α, interleukin-1β, and interleukin-6 in bronchoalveolar lavage fluid. Furthermore, Western blot analysis showed that PA inhibited the phosphorylation of IκB-α and p65 nuclear factor κB (NF-κB) induced by LPS.
  
  
  CONCLUSIONS
  Our results suggest that the anti-inflammatory effects of PA against LPS-induced ALI may be due to its ability to inhibit NF-κB signaling pathways.
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yu
authors.affiliation: Department of Clinical Laboratory, Qilu Hospital, Shandong University, Jinan, Shandong, China.
authors.forename: Xiao-Shi
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xue
authors.affiliation: Department of Pharmacy, The Second Hospital of Shandong University, Jinan, Shandong, China. Electronic address: wangrongmeiyl@163.com.
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D019686:Lamiaceae; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D012717:Sesquiterpenes; D009195:Peroxidase; C052983:patchouli alcohol
keywords: Acute lung injury; LPS; NF-κB; Patchouli alcohol