title: Histopathological and biochemical evidence of hepatopancreatic toxicity caused by cadmium in white shrimp, Palaemonetes argentinus.
abstract: Cadmium (Cd) is one of the most common pollutants in the environment and induces a range of tissue changes or damages and organ dysfunction. The histopathological effects of Cd and lipid peroxidation (LPO) on hepatopancreas of the freshwater shrimp, Palaemonetes argentinus, were studied. Shrimp were obtained from two lagoons with contrasting environmental quality, De los Padres (LP, impacted site) and Nahuel Rucá (NR, reference site), and were exposed to 3.06 and 12.24µgCdL(-1) for 3, 7, 10 and 15 days. The health status of both populations was also evaluated by histological analysis of control individuals. After exposure, shrimp were transferred to clean water for 28 days to evaluate the recuperation capacity of hepatopancreas. Control shrimp from NR exhibited a normal hepatopancreas structure; unlike control shrimp from LP which showed several alterations. These results were attributed to the different environmental quality of lagoons. The exposure to Cd resulted in several alterations in the histological structure of the hepatopancreas of both populations. The observed alterations included haemocytic and connective infiltrations in the intertubular space, erosioned microvilli, ripple of basal lamina, atrophied epithelium and necrosis, however, the latter was only observed in shrimp from LP. The exposure also caused an increase of LPO levels in both populations. P. argentinus was able to repair the hepatopancreas structure from the damage caused by Cd, evidenced by the histopathological results and LPO levels. Obtained results are indicating that the histological analysis of the hepatopancreas proved to be a highly sensitive method for evaluating water quality, in both environmental and laboratory conditions.
authors.affiliation: Toxicología Ambiental, Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina. Electronic address: leilachiodi@gmail.com.ar.
authors.forename: L N
authors.initials: LN
authors.lastname: Chiodi Boudet
authors.affiliation: Toxicología Ambiental, Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Polizzi
authors.affiliation: Toxicología Ambiental, Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: M B
authors.initials: MB
authors.lastname: Romero
authors.affiliation: Toxicología Ambiental, Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Robles
authors.affiliation: Área de Oceanografía Química, Instituto Argentino de Oceanografía (IADO- CONICET/UNS), Bahía Blanca, Argentina.
authors.forename: J E
authors.initials: JE
authors.lastname: Marcovecchio
authors.affiliation: Toxicología Ambiental, Facultad de Ciencias Exactas y Naturales, Departamento de Ciencias Marinas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina; Instituto de Investigaciones Marinas y Costeras (IIMyC), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: M S
authors.initials: MS
authors.lastname: Gerpe
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D004777:Environment; D004848:Epithelium; D005618:Fresh Water; D015386:Hazardous Substances; D043143:Hepatopancreas / Q000187:drug effects* / Q000473:pathology; D007753:Laboratories; D015227:Lipid Peroxidation / Q000187:drug effects*; D008871:Microvilli; D009336:Necrosis; D033541:Palaemonidae / Q000187:drug effects*; D033561:Penaeidae; D014867:Water; D014876:Water Pollution / Q000009:adverse effects*; D060753:Water Quality
chemical_list: D015386:Hazardous Substances; D002104:Cadmium; D014867:Water
keywords: Cadmium; Freshwater shrimp; Histopathology; Lipid peroxidation; Sublethal toxicity