title: Identification and expression analysis of QM-like gene from Spodoptera litura after challenge by the entomopathogenic fungus Nomuraea rileyi.
abstract: A partial sequence of QM homologue was isolated from a Spodoptera litura fatbody suppression subtractive hybridization library. The full-length Spodoptera litura QM (SpLQM) cDNA of 838 bp contains a 5' untranslated region (UTR) of 112 bp, a 3' UTR of 66 bp, and an open reading frame of 660 nucleotides coding for a 219 amino acid peptide with a molecular weight of 25.5 kDa. Analysis of SpLQM sequence revealed the presence of characteristic motifs, including the ribosomal protein L10 signature and SH3-binding motif. Multiple alignment analysis revealed that SpLQM shares an overall identity of 57.1-99.1% with other members of QM family. Phylogenetic analysis confirmed that SpLQM is closely related to other insect QMs. Analysis of the tissue expression pattern showed that the SpLQM mRNA was expressed in all tissues tested, with highest levels measured in hemocytes, followed by fat bodies. Upon Nomuraea rileyi challenge, SpLQM showed significant upregulation in fat bodies and hemocytes, while slightly upregulation in midguts. The results suggest that SpLQM might play an important role in the innate immunity of S. litura in response to N. rileyi infection. SpLQM was also successfully overexpressed in Escherichia coli, and the recombinant fusion protein SpLQM-His has a molecular weight of 32 kDa.
authors.affiliation: Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Zhong-Kang
authors.initials: ZK
authors.lastname: Wang
authors.affiliation: Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing 400030, China.
authors.forename: Er-Yan
authors.initials: EY
authors.lastname: Feng
authors.affiliation: Chongqing Engineering Research Center for Fungal Insecticides, School of Life Science, Chongqing University, Chongqing 400030, China ypy128@sina.com.
authors.forename: You-Ping
authors.initials: YP
authors.lastname: Yin
mesh_terms: D000596:Amino Acids; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D005216:Fat Body / Q000378:metabolism / Q000382:microbiology; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000378:metabolism* / Q000382:microbiology*; D006999:Hypocreales / Q000502:physiology*; D007113:Immunity, Innate / Q000276:immunology; D007814:Larva / Q000378:metabolism / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger; D000080882:Ribosomal Protein L10; D012269:Ribosomal Proteins / Q000378:metabolism; D018411:Spodoptera / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins
keywords: QM gene; gene expression; innate immunity