title: The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP.
abstract: The smallest gene HBx of Hepatitis B virus (HBV) is recognized as an important viral oncogene (V-oncogene) in the hepatocarcinogenesis. Our previous work demonstrated that RMP is a cellular oncogene (C-oncogene) required for the proliferation of hepatocellular carcinoma (HCC) cells. Here we presented the collaboration between V-oncogene HBx and C-oncogene RMP in the development of HCC. The coexpression of HBx and RMP resulted in the cooperative effect of antiapoptosis and proliferation of HCC cells. In vivo, overexpression of RMP accelerated the growth of HBx-induced xenograft tumors in nude mice and vice versa HBx promoted the growth of RMP-driven xenograft tumors. Although HBx didn't regulate the expression of RMP, HBx and RMP interact with each other and collocalized in the cytoplasm of HCC cells. HBx and RMP collaboratively inhibited the expression of apoptotic factors and promoted the expression of antiapoptotic factors. This finding suggests that HBV may induce, or at least partially contributes to the carcinogenesis of HCC, through its V-oncoprotein HBx interacting with the C-oncoprotein RMP.
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China ; 2. Department of Tumor Biotherapy, Third Affiliated Hospital of Soochow University, Changzhou, 213003 China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
authors.affiliation: 3. Eastern Hepatobiliary Surgery Hospital, Shanghai, 200433, China.
authors.forename: Zengqing
authors.initials: Z
authors.lastname: Wen
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Yu
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Shaomu
authors.initials: S
authors.lastname: Chen
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Han
authors.initials: H
authors.lastname: Chen
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Qinying
authors.initials: Q
authors.lastname: She
authors.affiliation: 2. Department of Tumor Biotherapy, Third Affiliated Hospital of Soochow University, Changzhou, 213003 China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Jiang
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Miao
authors.affiliation: 1. Department of Cell Biology, School of Medicine, Soochow University, Suzhou, 215123 China.
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Wei
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008113:Liver Neoplasms / Q000235:genetics*; D051379:Mice; D008856:Microscopy, Fluorescence; D009856:Oncogene Proteins, Viral / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D017931:DNA Primers; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D015534:Trans-Activators; C485517:Uri1 protein, mouse; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein
keywords: HBx; viral oncogene