title: Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction.
abstract: Wound dressing is critical important for cutaneous wound healing. However, the application of current products is limited due to poor mechanical property, unsuitable water vapor transmission rate (WVTR), poor anti-infective property or poor biocompatibility, etc. In the present study, a microporous silicone rubber membrane bilayer (SRM-B) composed of two layers with different pore sizes was prepared. The physical properties, the influences of pore structure on the bacterial penetration, the cell adhesion and proliferation were studied. Lastly, the effects of the SRM-B on the healing of a mouse full-thickness wound were examined. The data showed that the small pore upper layer of SRM-B could effectively prevent the bacterial invasion, as well as properly keep the water vapor transmission rate; the large pore lower layer of SRM-B could promote the cell adhesion and proliferation. The in vivo results showed that SRM-B could significantly enhance wound re-epithelialization and contraction, which accelerated the wound healing. Our data suggested that the SRM-B, with different particular pore sizes, could serve as a kind of promising wound dressing.
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xu
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China. Electronic address: logxw@yahoo.com.
authors.forename: Gaoxing
authors.initials: G
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Hesheng
authors.initials: H
authors.lastname: Xia
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: He
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Polymer Materials Engineering, Polymer Research Institute of Sichuan University, Chengdu 610065, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Jianglin
authors.initials: J
authors.lastname: Tan
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Daisong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Rixing
authors.initials: R
authors.lastname: Zhan
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Yang
authors.affiliation: Institute of Burn Research, Southwest Hospital, State Key Lab of Trauma, Burn and Combined Injury, Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China. Electronic address: editorinchief@burninchina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D001458:Bandages; D002448:Cell Adhesion; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006097:Granulation Tissue / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008051:Lipid Bilayers / Q000494:pharmacology*; D055595:Mechanical Phenomena; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D008855:Microscopy, Electron, Scanning; D018919:Neovascularization, Physiologic / Q000187:drug effects; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D016062:Porosity; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D062326:Re-Epithelialization / Q000187:drug effects*; D012826:Silicone Elastomers / Q000494:pharmacology*; D013227:Steam; D013997:Time Factors
chemical_list: D008051:Lipid Bilayers; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018809:Proliferating Cell Nuclear Antigen; D012826:Silicone Elastomers; D013227:Steam; D049452:Green Fluorescent Proteins
keywords: Bilayer; Micropore; Silicone rubber; Wound dressing; Wound healing