title: Neuroprotective effect of prenylated arylbenzofuran and flavonoids from morus alba fruits on glutamate-induced oxidative injury in HT22 hippocampal cells.
abstract: A prenylated arylbenzofuran and six flavonoids were isolated from the fruits of Morus alba L. through silica gel, octadecyl silica gel, and Diaion HP-20 column chromatography. Based on the nuclear magnetic resonance, mass spectrometry, and infrared spectroscopic data, the chemical structures of the compounds were determined to be artoindonesianin O (1), isobavachalcone (2), morachalcone A (3), quercetin (4), astragalin (5), isoquercetin (6), and rutin (7). The isolated compounds were evaluated for protection of HT22-immortalized hippocampal cells against glutamate-induced oxidative stress. Compounds 1 and 3 exhibited protective effects with EC(50) values of 19.7±1.2 and 35.5±2.1 μM, respectively. The major compounds 1-3 and 7 were quantified using liquid chromatography/mass spectrometry analysis and were determined to be 1.88±2.1, 1.90±1.8, 0.78±1.5, and 37.29±2.2 mg/kg, respectively, in the ethanol extract of M. alba L. fruits.
authors.affiliation: 1 Department of Oriental Medicinal Materials and Processing, Graduate School of Biotechnology, Kyung-Hee University , Yongin, Korea.
authors.forename: Kyeong-Hwa
authors.initials: KH
authors.lastname: Seo
authors.forename: Dae-Young
authors.initials: DY
authors.lastname: Lee
authors.forename: Rak-Hun
authors.initials: RH
authors.lastname: Jeong
authors.forename: Dong-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Young-Eon
authors.initials: YE
authors.lastname: Kim
authors.forename: Eock-Kee
authors.initials: EK
authors.lastname: Hong
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Nam-In
authors.initials: NI
authors.lastname: Baek
mesh_terms: D002460:Cell Line; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005638:Fruit / Q000737:chemistry; D005663:Furans / Q000737:chemistry / Q000494:pharmacology*; D018698:Glutamic Acid / Q000009:adverse effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D013058:Mass Spectrometry; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D005419:Flavonoids; D005663:Furans; D018696:Neuroprotective Agents; D010936:Plant Extracts; D018698:Glutamic Acid; C039281:furan
keywords: HT22; Morus alba L.; artoindonesianin O; morachalcone A; neuroprotection effect; quantitative analysis