title: A second-generation phosphohistidine analog for production of phosphohistidine antibodies.
abstract: Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.
authors.affiliation: Department of Chemistry, Princeton University , Princeton, New Jersey 08544, United States.
authors.forename: Jung-Min
authors.initials: JM
authors.lastname: Kee
authors.forename: Rob C
authors.initials: RC
authors.lastname: Oslund
authors.forename: Anthony D
authors.initials: AD
authors.lastname: Couvillon
authors.forename: Tom W
authors.initials: TW
authors.lastname: Muir
mesh_terms: D045163:Antibodies, Phospho-Specific / Q000737:chemistry*; D006639:Histidine / Q000031:analogs & derivatives* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry*; D011720:Pyrazoles / Q000138:chemical synthesis* / Q000737:chemistry; D015398:Signal Transduction; D014230:Triazoles / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D045163:Antibodies, Phospho-Specific; D011506:Proteins; D011720:Pyrazoles; D014230:Triazoles; D006639:Histidine; C005436:phosphohistidine