title: MicroRNA-103a inhibits gastric cancer cell proliferation, migration and invasion by targeting c-Myb.
abstract: OBJECTIVE
  There have been no previous reports concerning functions of miR-103a in gastric cancer (GC) cells. Thus the aim of the study was to investigate its expression and role in development of this tumour.
  
  
  METHODS
  Real-time RT-PCR was performed to detect expression of miR-103a in GC cell lines and clinical cancer specimens. To further understand its role, we restored expression of miR-103a in MGC-803 cell line by transfection with miR-103a mimics or inhibitors. Effects of miR-103a on cell proliferation, migration and invasion on targets were also determined.
  
  
  RESULTS
  miR-103a was down-regulated in both GC cell lines and clinical cancer specimens. Meanwhile, its level was closely associated with pM or pTNM stage of GC. Overexpression of miR-103a markedly suppressed proliferation, migration, and invasion of GC cells, while its inhibition significantly accelerated cell proliferation, migration and invasion. Moreover, c-Myb was identified to be a functional downstream target of miR-103a, ectopic expression of which partially reversed suppression of cell proliferation and invasion.
  
  
  CONCLUSIONS
  Thus our observations suggest that miR-103a functioned as a tumour suppressor by targeting c-Myb. These findings indicate that miR-103a might play a significant role in pathogenesis of GC.
authors.affiliation: Department of General Surgery, Heilongjiang Province Hospital, Harbin, Heilongjiang, 150000, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Liang
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Liu
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Xue
authors.forename: Bing
authors.initials: B
authors.lastname: Qiu
authors.forename: Bin
authors.initials: B
authors.lastname: Wei
authors.forename: Kan
authors.initials: K
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics* / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: C544749:MIRN103 microRNA, human; D035683:MicroRNAs; D020598:Proto-Oncogene Proteins c-myb