title: Antipapain activity in the serum of patients with breast cancer.
abstract: BACKGROUND
  Complement information about the share the role of antipapain activity in serum people with breast cancer.
  
  
  OBJECTIVE
  We measured the activity of cysteine peptidase inhibitors in the sera of 150 patients with breast cancer. Patients were divided into four groups depending on the cancer type and treatment method. We also analysed the control group. The activity of cysteine peptidase inhibitors was defined as a 'defensiveness' marker.
  
  
  METHODS
  The activity of cysteine peptidase inhibitors was measured against papain using the colorimetric method and the BANA substrate.
  
  
  RESULTS
  The highest activity of enzymes was found in the group of patients with BC and hereditary predisposition to it, and the lowest activity was found in patients after surgical treatment.
  
  
  CONCLUSIONS
  The activity of cysteine peptidase inhibitors in serum was measured against papain. We found that the activity levels were correlated with the cancer stage and treatment method. The lowest activity was found in patients after surgical treatment; the highest in women with active cancer and a hereditary predisposition to it.
authors.affiliation: Department of Oncology, District Hospital, Leśna, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kilar
authors.affiliation: Department of Anatomy, Wrocław Medical University, Wrocław, Poland.
authors.forename: Bohdan
authors.initials: B
authors.lastname: Gworys
authors.affiliation: Department of Animal Product Technology and Quality Management, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
authors.forename: Teresa
authors.initials: T
authors.lastname: Skiba
authors.affiliation: Department of Pharmaceutical Biochemistry, Wrocław Medical University, Wrocław, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Gburek
authors.affiliation: Department of Pharmaceutical Biochemistry, Wrocław Medical University, Wrocław, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Gołąb
authors.affiliation: Department of Physiology, Wrocław Medical University, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Janocha
authors.affiliation: Department of Basic Sciences, Wrocław Medical University, Wrocław, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Siewiński
mesh_terms: D001943:Breast Neoplasms / Q000097:blood* / Q000201:enzymology* / Q000601:surgery; D003124:Colorimetry / Q000379:methods; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D010206:Papain / Q000037:antagonists & inhibitors* / Q000097:blood; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood
chemical_list: D015853:Cysteine Proteinase Inhibitors; D010206:Papain; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
keywords: Breast cancer; antipapain activity