title: QCM-D providing new horizon in the domain of sensitivity range and information for haemostasis of human plasma.
abstract: Monitoring of the haemostasis status is significant for proper therapeutic directions and decisions in surgery and innate coagulation disorders. In this regard, to gain a general overview of the plasmatic coagulation, prothrombin time (PT) tests are frequently combined with tests for activated partial thromboplastin time (aPTT). For aPTT we report for the first time that a QCM-D (Quartz Crystal Microbalances with Dissipation) based technique offers a better alternative to the standard coagulometer method in the perspective of range and information. We used heparin as anticoagulant to generate different coagulation times for human plasma. QCM-D astonishingly proved to be more sensitive and reliable than the standard coagulometer for aPTT range of upper limits of coagulation times. The established platform can monitor the fibrinogen concentration ranging from 1-6g/L (yielding R(2)=0.98 in calibration curves) along with aPTT from frequency and dissipation shifts together in a single set of measurements. Additionally the sensor layers have been tested for reusability, demonstrating no loss in sensor characteristics up to ten times measurements.
authors.affiliation: Biosensor Research Group, Institute of Clinical and Experimental Transfusion Medicine and Centre of Clinical Transfusion Medicine (ZKT), Tuebingen University and German Red Cross Blood Transfusion Service BW/H, Germany. Electronic address: munawar_arif@hotmail.com.
authors.forename: Munawar
authors.initials: M
authors.lastname: Hussain
authors.affiliation: Biosensor Research Group, Institute of Clinical and Experimental Transfusion Medicine and Centre of Clinical Transfusion Medicine (ZKT), Tuebingen University and German Red Cross Blood Transfusion Service BW/H, Germany.
authors.forename: Hinnak
authors.initials: H
authors.lastname: Northoff
authors.affiliation: Biosensor Research Group, Institute of Clinical and Experimental Transfusion Medicine and Centre of Clinical Transfusion Medicine (ZKT), Tuebingen University and German Red Cross Blood Transfusion Service BW/H, Germany. Electronic address: frank.gehring@med.uni-tuebingen.de.
authors.forename: Frank K
authors.initials: FK
authors.lastname: Gehring
mesh_terms: D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D001780:Blood Coagulation Tests / Q000379:methods / Q000706:statistics & numerical data; D002138:Calibration; D005340:Fibrinogen / Q000032:analysis; D006487:Hemostasis; D006801:Humans; D010314:Partial Thromboplastin Time / Q000379:methods* / Q000706:statistics & numerical data; D010976:Platelet Count; D011517:Prothrombin Time / Q000379:methods / Q000706:statistics & numerical data; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results
chemical_list: D005340:Fibrinogen
keywords: Fibrinogen; Plasma; QCM-D; aPTT; qCell T