title: MicroRNA profiling in human colon cancer cells during 5-fluorouracil-induced autophagy.
abstract: Autophagy modulation is now recognized as a potential therapeutic approach for cancer (including colorectal cancer), yet the molecular mechanisms regulating autophagy in response to cellular stress are still not well understood. MicroRNAs (miRNAs) have been found to play important roles in controlling many cellular functions, including growth, metabolism and stress response. The physiological importance of the miRNA-autophagy interconnection is only beginning to be elucidated. MiRNA microarray technology facilitates analysis of global miRNA expression in certain situations. In this study, we explored the expression profile of miRNAs during the response of human colon cancer cells (HT29s) to 5-FU treatment and nutrient starvation using miRNA microarray analysis. The alteration of miRNA expression showed the same pattern under both conditions was further testified by qRT-PCR in three human colon cancer cell lines. In addition, bioinformatic prediction of target genes, pathway analysis and gene network analysis were performed to better understand the roles of these miRNAs in the regulation of autophagy. We identified and selected four downregulated miRNAs including hsa-miR-302a-3p and 27 upregulated miRNAs under these two conditions as having the potential to target genes involved in the regulation of autophagy in human colon cancer cells. They have the potential to modulate autophagy in 5-FU-based chemotherapy in colorectal cancer.
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Hou
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Yingxun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Yannan
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Tusheng
authors.initials: T
authors.lastname: Song
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University School of Medicine, Xi'an, China; Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University School of Medicine, Xi'an, China; Cardiovascular Research Center, Xi'an Jiaotong University School of Medicine, Xi'an, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D001343:Autophagy; D003110:Colonic Neoplasms / Q000378:metabolism*; D005472:Fluorouracil / Q000633:toxicity; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D005472:Fluorouracil