title: MicroRNA-134 suppresses endometrial cancer stem cells by targeting POGLUT1 and Notch pathway proteins.
abstract: We aimed to ascertain the role of microRNAs (miRNAs) in regulating human endometrial cancer stem cells (HuECSCs). The expression level of miRNA-134 (miR-134), a member of the DLK1-DIO3 genomic imprinted miRNA cluster, differed significantly between HuECSCs and human endometrial cancer cells (HuECCs). miR-134 inhibited HuECSCs proliferation and migration by targeting protein O-glucosyltransferase 1 (POGLUT1) expression. Exogenous miR-134 overexpression downregulated POGLUT1 and Notch pathway proteins in HuECSCs in vitro. miR-134 overexpression affected the G2/M phase of HuECSCs and suppressed the growth of xenograft tumours formed. Thus, endogenous miR-134 regulation in HuECSCs may suppress tumourigenesis in human endometrial carcinoma.
authors.affiliation: International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Gao
authors.affiliation: International Peace Maternity and Child Health Hospital, Shanghai Jiaotong University, Shanghai 200030, China; Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai 200072, China; Shanghai Geriatric Institute of Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, China. Electronic address: teliu79@126.com.
authors.forename: Te
authors.initials: T
authors.lastname: Liu
authors.affiliation: Laboratoire PROTEE, Bâtiment R, Université du Sud Toulon-Var, 83957 LA GARDE Cedex, France.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Huang
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D051880:Receptors, Notch / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C569273:MIRN134 microRNA, human; D035683:MicroRNAs; D051880:Receptors, Notch; D005964:Glucosyltransferases; C510651:POGLUT1 protein, human
keywords: DLK1-DIO3 genomic imprinted microRNA cluster; Human endometrial cancer stem cell; MicroRNA-134; Migration; Notch pathway; Proliferation; Protein O-glucosyltransferase 1