title: Simultaneous pretreatment and saccharification: green technology for enhanced sugar yields from biomass using a fungal consortium.
abstract: Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Saurabh Sudha
authors.initials: SS
authors.lastname: Dhiman
authors.affiliation: Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Rim
authors.initials: JR
authors.lastname: Haw
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea.
authors.forename: Dayanand
authors.initials: D
authors.lastname: Kalyani
authors.affiliation: Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi 110007, India.
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
authors.affiliation: Department of Materials Science and Engineering, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 136-713, Republic of Korea.
authors.forename: Yun Chan
authors.initials: YC
authors.lastname: Kang
authors.affiliation: Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea; Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Republic of Korea. Electronic address: jkrhee@konkuk.ac.kr.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000096:biosynthesis*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D055772:Green Chemistry Technology / Q000379:methods*; D006868:Hydrolysis; D059013:Microbial Consortia; D018625:Microscopy, Atomic Force; D012275:Oryza / Q000737:chemistry; D010636:Phenols / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D013501:Surface-Active Agents / Q000494:pharmacology; D014866:Waste Products / Q000032:analysis
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D010636:Phenols; D013501:Surface-Active Agents; D014866:Waste Products; D000431:Ethanol
keywords: Ethanol; Fungal consortia; Pretreatment; Reducing sugars; Saccharification