title: Clinical-instrumental and morphological evaluation of the effect of autologous dermal fibroblasts administration.
abstract: Basic molecular mechanisms, associated with the main cell population of the dermis - fibroblasts - are the basis of skin aging. The number of functionally active fibroblasts in the skin and their biosynthetic activity decreases with age, thus enhancement of their cell density with synthetically active cells is accepted as a one of the most effective methods. The objective of the present study was to evaluate the safety and effectiveness of intradermal administration of autologous dermal fibroblasts in a year after treatment of 17 patients, aged 45-65 years. Results obtained with modern instrumental skin diagnostic methods (vacuum cutometry, optical profilometry, VISIA photometric analysis, etc.) demonstrate the safety and clinical effectiveness of dermal autofibroblast therapy: after transplantation, cultured autofibroblasts keep their biosynthetic activity and produce extracellular matrix for at least 12 months. As a result, remodelling of the dermis microstructures is observed, accompanied by a progressive increase of collagen content and thickness of the dermis (up to 62.5 ±6.7% in 12 months). This is clinically expressed by increase of skin elasticity (24.0 ±4.3% in periorbital area) and thickness of the skin, and by decrease in the number and depth of wrinkles (46 ±7% by the end of observation period). Copyright © 2014 John Wiley & Sons, Ltd.
authors.affiliation: OJSC 'Human Stem Cells Institute', Business Development Department, Moscow, Russia.|Research Institute of carcinogenesis, RAMS, Moscow, Russia.
authors.forename: Vadim
authors.initials: V
authors.lastname: Zorin
authors.affiliation: OJSC 'Human Stem Cells Institute', Regenerative Medicine Department, Moscow, Russia.
authors.forename: Alla
authors.initials: A
authors.lastname: Zorina
authors.affiliation: OJSC 'Human Stem Cells Institute', Regenerative Medicine Department, Moscow, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Cherkasov
authors.affiliation: OJSC 'Human Stem Cells Institute', R&D Department, Moscow, Russia.
authors.forename: Roman
authors.initials: R
authors.lastname: Deev
authors.affiliation: Research Institute of carcinogenesis, RAMS, Moscow, Russia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Kopnin
authors.affiliation: OJSC 'Human Stem Cells Institute', Business Development Department, Moscow, Russia.
authors.forename: Artur
authors.initials: A
authors.lastname: Isaev
mesh_terms: D000368:Aged; D003114:Colony-Forming Units Assay; D020405:Dermis / Q000166:cytology*; D004548:Elasticity; D005145:Face; D005260:Female; D005347:Fibroblasts / Q000166:cytology* / Q000637:transplantation*; D006801:Humans; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D015595:Skin Aging / Q000502:physiology; D014182:Transplantation, Autologous
keywords: SPRS therapy; aging of skin; autologous fibroblasts; cell therapy; regenerative medicine