title: Identification of differentially-expressed genes in intestinal gastric cancer by microarray analysis.
abstract: Gastric cancer (GC) is one of the most frequent malignant tumors. In order to systematically characterize the cellular and molecular mechanisms of intestinal GC development, in this study, we used 22K oligonucleotide microarrays and bioinformatics analysis to evaluate the gene expression profiles of GC in 45 tissue samples, including 20 intestinal GC tissue samples, 20 normal appearing tissues (NATs) adjacent to tumors and 5 noncancerous gastric mucosa tissue samples. These profiles allowed us to explore the transcriptional characteristics of GC and determine the change patterns in gene expression that may be of clinical significance. 1519 and 1255 differentially-expressed genes (DEGs) were identified in intestinal GC tissues and NATs, respectively, as determined by Bayesian analysis (P<0.001). These genes were associated with diverse functions such as mucosa secretion, metabolism, proliferation, signaling and development, which occur at different stages of GC development.
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Shizhu
authors.initials: S
authors.lastname: Zang
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Ruifang
authors.initials: R
authors.lastname: Guo
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Wenmei
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, Renji Hospital of Shanghai Second Medical University, Shanghai 200001, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Gastroenterology, Peking University First Hospital, Beijing 100034, China.
authors.forename: Fulian
authors.initials: F
authors.lastname: Hu
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Kang
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Ren
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Beijing Institutes of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York 10029, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Pathology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Li
authors.affiliation: Shanghai Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200025, China.
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Engineering Research Center for Beijing Biochip Technology and Tsinghua University School of Medicine, Beijing 102206, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Laboratory of Molecular Oncology, MOE Key Laboratory of Carcinogenesis and Translational Research, Peking University Cancer Hospital & Institute, Beijing 100142, China. Electronic address: youyonglu@bjmu.edu.cn.
authors.forename: Youyong
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001499:Bayes Theorem; D014408:Biomarkers, Tumor / Q000235:genetics*; D005753:Gastric Mucosa / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics*; D046228:Microarray Analysis; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor
keywords: Gastric cancer development; Gene expression profile; Microarray