title: [Human lymphocyte immunophenotype after interaction with mesenchymal stromal cells].
abstract: Human multipotent mesenchymalstromal cells were cocultured for 72h with allogeneic blood-borne mononuclear cells (MNCs) of different maturity (lymphocytes from adult peripheral blood and umbilical cord blood) and functional state (intact and PHA-activated human peripheral blood lymphocytes): After coculture with MMSCs the share ofB-cells among MNCs and cbMNCs decreased. The proportion ofT- and NK cells declined only among cbMNCs (p < 0.05). Only T-NK subpopulation reduced among MNC and cbMNC T-cells. The drop of CD8+ cells was detected in coculture of MMSCs and PHA-MNCs. Activated HLA-DR+ cells declined, CD25+ cells increased compared to monoculture of PHA-MNCs. MMSCs supported MNC, PHA-MNC and cbMNC viability. These results are important for understanding the physiology of the allogeneic cell-to-cell interaction in connection of potential clinical application of allogeneic cell products of blood-borne and stroinal origin.
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: I V
authors.initials: IV
authors.lastname: Andrianova
authors.forename: P I
authors.initials: PI
authors.lastname: Bobyleva
authors.forename: A N
authors.initials: AN
authors.lastname: Gornostaeva
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication / Q000276:immunology*; D002470:Cell Survival / Q000276:immunology; D018920:Coculture Techniques; D006801:Humans; D007694:Killer Cells, Natural; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*