title: Noninvasive assessment of pulse-wave velocity and flow-mediated vasodilation in anesthetized Göttingen minipigs.
abstract: Few methods for noninvasive assessment of arterial stiffness and endothelial dysfunction in porcine models are available. The aim of this study was to evaluate methods for assessment of arterial stiffness and endothelial dysfunction in anesthetized Göttingen minipigs. Pulse-wave velocity (PWV) was assessed in male Göttingen minipigs (n = 8; age approximately 60 wk) by using applanation tonometry of the carotid and femoral arteries. In addition, flow-mediated vasodilation (FMD) was assessed by using vascular ultrasonography of the brachial artery to evaluate endothelial dysfunction. To evaluate the reproducibility of the methods, minipigs were anesthetized by intravenous infusion of ketamine and midazolam and examined every other day for a total of 3 trials. Neither examination day nor systolic, diastolic, or mean arterial blood pressure statistically influenced PWV or FMD. The median interexamination coefficient of variation was 17% for PWV and 59% for FMD. Measured values of PWV corresponded largely to those in clinically healthy humans, but FMD values were lower than expected for lean, young animals. Although the ketamine-midazolam anesthesia we used has been associated with minor hemodynamic effects in vivo, in vitro studies suggest that both drugs are vasodilatory. Therefore anesthesia might have influenced the endothelial response, contributing to the modest FMD response and the concurrent high coefficients of variation that we noted. We conclude that PWV—but not FMD—showed acceptable interexamination variation for its potential application in porcine models.
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark; Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Trine P
authors.initials: TP
authors.lastname: Ludvigsen
authors.affiliation: Department of Clinical Physiology and Nuclear Medicine, Frederiksberg Hospital, Frederiksberg, Denmark.
authors.forename: Niels
authors.initials: N
authors.lastname: Wiinberg
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Christina J
authors.initials: CJ
authors.lastname: Jensen
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Annemette T
authors.initials: AT
authors.lastname: Callesen
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Regitze W
authors.initials: RW
authors.lastname: Andersen
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: Anne Sofie H
authors.initials: AS
authors.lastname: Jørgensen
authors.affiliation: Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Berit Ø
authors.initials: BØ
authors.lastname: Christoffersen
authors.affiliation: Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Henrik D
authors.initials: HD
authors.lastname: Pedersen
authors.affiliation: Novo Nordisk A/S, Måløv, Denmark.
authors.forename: Sophia G
authors.initials: SG
authors.lastname: Moesgaard
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen, Frederiksberg, Denmark. lisbeth.hoier@sund.ku.dk.
authors.forename: Lisbeth H
authors.initials: LH
authors.lastname: Olsen
mesh_terms: D000758:Anesthesia; D000818:Animals; D001794:Blood Pressure; D004727:Endothelium / Q000503:physiopathology*; D007649:Ketamine; D008297:Male; D008874:Midazolam; D063177:Pulse Wave Analysis / Q000379:methods*; D013552:Swine; D013556:Swine, Miniature / Q000502:physiology*; D059289:Vascular Stiffness / Q000502:physiology*; D014664:Vasodilation / Q000502:physiology*
chemical_list: D007649:Ketamine; D008874:Midazolam