title: A device for dried blood microsampling in quantitative bioanalysis: overcoming the issues associated blood hematocrit.
abstract: OBJECTIVE
  A cross-laboratory experiment has been performed on a novel dried blood sampler in order to investigate whether it overcomes issues associated with blood volume and hematocrit (HCT) that are observed when taking a subpunch from dried blood spot samples.
  
  
  METHODS
  An average blood volume of 10.6 μl was absorbed by the samplers across the different HCTs investigated (20-65%).
  
  
  RESULTS
  No notable change of volume absorbed was noted across the HCT range. Furthermore, the variation in blood sample volumes across six different laboratories was within acceptable limits.
  
  
  CONCLUSIONS
  The novel volumetric absorptive microsampling device has the potential to deliver the advantages of dried blood spot sampling while overcoming some of the issues associated with the technology.
authors.affiliation: Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Spooner
authors.forename: Philip
authors.initials: P
authors.lastname: Denniff
authors.forename: Luc
authors.initials: L
authors.lastname: Michielsen
authors.forename: Ronald
authors.initials: R
authors.lastname: De Vries
authors.forename: Qin C
authors.initials: QC
authors.lastname: Ji
authors.forename: Mark E
authors.initials: ME
authors.lastname: Arnold
authors.forename: Karen
authors.initials: K
authors.lastname: Woods
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Woolf
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Valérie
authors.initials: V
authors.lastname: Boutet
authors.forename: Patricia
authors.initials: P
authors.lastname: Zane
authors.forename: Stuart
authors.initials: S
authors.lastname: Kushon
authors.forename: James B
authors.initials: JB
authors.lastname: Rudge
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D016477:Artifacts; D001800:Blood Specimen Collection / Q000295:instrumentation*; D059788:Dried Blood Spot Testing / Q000295:instrumentation*; D006400:Hematocrit; D006801:Humans; D051381:Rats