title: Genetic and biochemical evaluation of natural rubber from Eastern Washington prickly lettuce (Lactuca serriola L.).
abstract: Alternative sources of natural rubber are of importance due to economic, biological, and political threats that could diminish supplies of this resource. Prickly lettuce (Lactuca serriola L.) synthesizes long-chain natural rubber and was studied to determine underlying genetic and phenotypic characteristics of rubber biosynthesis. Genotypic and phenotypic analysis of an F2 segregating population using EST-SSR markers led to the discovery of genetic regions linked to natural rubber production. Interval mapping (IM) and multiple QTL mapping (MQM) identified several QTL in the mapping population that had significance based on LOD score thresholds. The discovered QTL and the corresponding local markers are genetic resources for understanding rubber biosynthesis in prickly lettuce and could be used in marker-assisted selection (MAS) breeding. Prickly lettuce is an excellent candidate for elucidating the rubber synthesis mechanism and has potential as a crop plant for rubber production.
authors.affiliation: Discovery Research, Dow Agrosciences, Indianapolis, Indiana, United States.
authors.forename: Jared L
authors.initials: JL
authors.lastname: Bell
authors.forename: Ian C
authors.initials: IC
authors.lastname: Burke
authors.forename: Michael M
authors.initials: MM
authors.lastname: Neff
mesh_terms: D019659:Asteraceae / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D001704:Biopolymers / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D005838:Genotype; D040641:Quantitative Trait Loci; D012408:Rubber / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D014861:Washington
chemical_list: D001704:Biopolymers; D012408:Rubber
keywords: EST-SSR; Lactuca serriola L.; QTL; polyisoprene; prickly lettuce; rubber