title: Production and structural characterization of Lactobacillus helveticus derived biosurfactant.
abstract: A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm(-1) pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL(-1). Low cost production of Lactobacilli derived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL(-1) biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR ((1)H and (13)C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced by L. helveticus MRTL91. These findings are encouraging for the application of Lactobacilli derived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications.
authors.affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra 136 119, India ; Dairy Microbiology Division, National Dairy Research Institute, Karnal 132 001, India.
authors.forename: Deepansh
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Microbiology, Kurukshetra University, Kurukshetra 136 119, India.
authors.forename: Baljeet Singh
authors.initials: BS
authors.lastname: Saharan
authors.affiliation: Division of Microbiology, Vector Control Research Center, Puducherry 605006, India.
authors.forename: Nikhil
authors.initials: N
authors.lastname: Chauhan
authors.affiliation: S. A. Jain College, Ambala City 134 002, India.
authors.forename: Anshul
authors.initials: A
authors.lastname: Bansal
authors.affiliation: Department of Chemistry, Kurukshetra University, Kurukshetra 136 119, India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Procha
mesh_terms: D000818:Animals; D018533:Biomass; D001937:Brassica / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000378:metabolism; D052198:Lactobacillus helveticus / Q000737:chemistry*; D007785:Lactose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D013500:Surface Tension / Q000187:drug effects; D013501:Surface-Active Agents / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D013818:Thermogravimetry; D014908:Triticum / Q000187:drug effects
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; D007785:Lactose