title: Bivalent ligation of the collagen-binding modules of fibronectin by SFS, a non-anchored bacterial protein of Streptococcus equi.
abstract: SFS is a non-anchored protein of Streptococcus equi subspecies equi that causes upper respiratory infection in horses. SFS has been shown to bind to fibronectin (FN) and block interaction of FN with type I collagen. We have characterized interactions of a recombinant 60-mer polypeptide, R1R2, with FN. R1R2 contains two copies of collagen-like 19-residue repeats. Experiments utilizing various FN fragments and epitope-mapped anti-FN monoclonal antibodies located the binding site to (8-9)FNI modules of the gelatin-binding domain. Fluorescence polarization and competitive enzyme-linked assays demonstrated that R1R2 binds preferentially to compact dimeric FN rather than monomeric constructs containing (8-9)FNI or a large dimeric FN construct that is constitutively in an extended conformation. In contrast to bacterial peptides that bind (2-5)FNI in addition to (8-9)FNI, R1R2 did not cause conformational extension of FN as assessed by a conformationally sensitive antibody. Equilibrium and stopped-flow binding assays and size exclusion chromatography were compatible with a two-step binding reaction in which each of the repeats of R1R2 interacts with one of the subunits of dimeric FN, resulting in a stable complex with a slow koff. In addition to not binding to type I collagen, the R1R2·FN complex incorporated less efficiently into extracellular matrix than free FN. Thus, R1R2 binds to FN utilizing features of compact soluble FN and in doing so interferes with the organization of the extracellular matrix. A similar bivalent binding strategy may underlie the collagen-FN interaction.
authors.affiliation: Departments of Biomolecular Chemistry and Medicine, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Wenjiang
authors.initials: W
authors.lastname: Ma
authors.affiliation: Departments of Biomolecular Chemistry and Medicine, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Hanqing
authors.initials: H
authors.lastname: Ma
authors.affiliation: Departments of Biomolecular Chemistry and Medicine, University of Wisconsin, Madison, Wisconsin 53706.
authors.forename: Frances J
authors.initials: FJ
authors.lastname: Fogerty
authors.affiliation: Departments of Biomolecular Chemistry and Medicine, University of Wisconsin, Madison, Wisconsin 53706. Electronic address: dfmosher@wisc.edu.
authors.forename: Deane F
authors.initials: DF
authors.lastname: Mosher
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry* / Q000235:genetics; D017434:Protein Structure, Tertiary; D018502:Streptococcus equi / Q000737:chemistry* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D005353:Fibronectins
keywords: Bacterial Protein; Collagen; FNI Modules; Fibronectin; Host-Pathogen Interactions; Intrinsically Disordered Protein; Kinetics; Matrix Metalloproteinase (MMP); Streptococcus equi