title: Blueberry treatment decreased D-galactose-induced oxidative stress and brain damage in rats.
abstract: D-galactose (GAL) causes aging-related changes and oxidative stress in the organism. We investigated the effect of whole fresh blueberry (BB) (Vaccinium corymbosum L.) treatment on oxidative stress in age-related brain damage model. Rats received GAL (300 mg/kg; s.c.; 5 days per week) alone or together with 5 % (BB1) and 10 % (BB2) BB containing chow for two months. Malondialdehyde (MDA),protein carbonyl (PC) and glutathione (GSH) levels, and Cu Zn-superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione transferase (GST) activities as well as acetylcholinesterase (AChE) activities were determined. Expressions of B cell lymphoma-2 (Bcl-2), Bax and caspase-3 were also evaluated in the brain by immunohistochemistry. MDA and PC levels and AChE activity increased, but GSH levels, SOD and GSH-Px activities decreased together with histopathological structural damage in the brain of GAL-treated rats. BB treatments, especially BB2 reduced MDA and PC levels and AChE activity and elevated GSH levels and GSH-Px activity. BB1 and BB2 treatments diminished apoptosis and ameliorated histopathological findings in the brain of GAL-treated rats. These results indicate that BB partially prevented the shift towards an imbalanced prooxidative status and apoptosis together with histopathological amelioration by acting as an antioxidant (radical scavenger) itself in GAL-treated rats.
authors.affiliation: Department of Biochemistry, Yeditepe University Medical Faculty, Kayışdağı, Istanbul, Turkey.
authors.forename: Jale
authors.initials: J
authors.lastname: Çoban
authors.forename: Işın
authors.initials: I
authors.lastname: Doğan-Ekici
authors.forename: A Fatih
authors.initials: AF
authors.lastname: Aydın
authors.forename: Esra
authors.initials: E
authors.lastname: Betül-Kalaz
authors.forename: Semra
authors.initials: S
authors.lastname: Doğru-Abbasoğlu
authors.forename: Müjdat
authors.initials: M
authors.lastname: Uysal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D029798:Blueberry Plants; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D005690:Galactose / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D005690:Galactose