title: Differential responses to steroid hormones in fibroblasts from the vocal fold, trachea, and esophagus.
abstract: There is accumulating evidence that fibroblasts are target cells for steroids such as sex hormones and corticoids. The characteristics of fibroblasts vary among tissues and organs. Our aim in this study is to examine differences in responses to steroid hormones among fibroblasts from different cervicothoracic regions. We compared the actions of steroid hormones on cultured fibroblasts from the vocal folds, which are considered to be the primary target of steroid hormones, and the trachea and esophagus in adult male rats. Expression of steroid hormone receptors (androgen receptor, estrogen receptor α, and glucocorticoid receptor) was identified by immunofluorescence histochemistry. Androgen receptor was much more frequently expressed in fibroblasts from the vocal fold than in those from the trachea and esophagus. Cell proliferation analysis showed that administration of testosterone, estradiol, or corticosterone suppressed growth of all 3 types of fibroblasts. However, mRNA expression for extracellular matrix-associated genes, including procollagen I and III and elastin, and hyaluronic acid synthase I was elevated only by addition of testosterone to fibroblasts from the vocal fold. These results indicate that each steroid hormone exerts region-specific effects on cervicothoracic fibroblasts with different properties through binding to specific receptors.
authors.affiliation: Departments of Anatomy and Neurobiology (S.M., K.I.M., M.K.) and Otolaryngology-Head and Neck Surgery (S.M., T.N., Y.S., H.B., R.H., H.S., Y.H.), Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan.
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Mukudai
authors.forename: Ken Ichi
authors.initials: KI
authors.lastname: Matsuda
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nishio
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Sugiyama
authors.forename: Hideki
authors.initials: H
authors.lastname: Bando
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Hirota
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sakaguchi
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Hisa
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Kawata
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D003345:Corticosterone / Q000494:pharmacology; D004947:Esophagus / Q000166:cytology*; D004958:Estradiol / Q000494:pharmacology; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism; D005109:Extracellular Matrix; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051381:Rats; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000235:genetics / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*; D013739:Testosterone / Q000494:pharmacology; D014132:Trachea / Q000166:cytology*; D014827:Vocal Cords / Q000166:cytology*
chemical_list: D047628:Estrogen Receptor alpha; D011944:Receptors, Androgen; D011965:Receptors, Glucocorticoid; D013256:Steroids; D013739:Testosterone; D004958:Estradiol; D003345:Corticosterone