title: DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection.
abstract: We report on a simple approach to enhance solid-phase hybridization-based single base mismatch discrimination at high ionic strength based on the deliberate insertion of a natural DNA base mismatch in the surface-tethered probe. A large drop in hybridization signal of single base mismatched alleles using the designed probe as compared with the conventional probe, from 80% to less than 25% of the signal obtained with the fully complementary, non-mutation-containing sequence, when using colorimetric detection was further improved to 20% when using electrochemical detection, attributable to a difference of spacing of immobilized probes. Finally, the designed probe was used for the electrochemical detection of the DQA1*05:05 allele amplified from real human blood samples.
authors.affiliation: INTERFIBIO Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Joda
authors.affiliation: INTERFIBIO Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: Valerio
authors.initials: V
authors.lastname: Beni
authors.affiliation: INTERFIBIO Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain.
authors.forename: Ioanis
authors.initials: I
authors.lastname: Katakis
authors.affiliation: INTERFIBIO Research Group, Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, 43007 Tarragona, Spain; Institucio Catalana de Recerca i Estudis Avançats, Passeig Lluis Companys 23, 08010 Barcelona, Spain. Electronic address: ciara.osullivan@urv.cat.
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D000483:Alleles; D020137:Base Pair Mismatch / Q000235:genetics*; D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry; D004247:DNA / Q000235:genetics*; D055664:Electrochemical Techniques; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000235:genetics*
chemical_list: D004247:DNA
keywords: DQA1*05:05 allele; Electrochemical genosensor; Hybridization refractory mutation system; Single base mismatch