title: The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality.
abstract: In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool.
authors.affiliation: Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, U0.11, Wilrijk 2610, Belgium. Electronic address: sara.valckx@uantwerpen.be.
authors.forename: Sara D M
authors.initials: SD
authors.lastname: Valckx
authors.affiliation: Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, U0.11, Wilrijk 2610, Belgium.
authors.forename: Jessie
authors.initials: J
authors.lastname: De Bie
authors.affiliation: Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, U0.11, Wilrijk 2610, Belgium.
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Michiels
authors.affiliation: Centre for Reproductive Medicine, University Hospital Antwerp, Wilrijkstraat 10, Edegem 2650, Belgium.
authors.forename: Ilse G
authors.initials: IG
authors.lastname: Goovaerts
authors.affiliation: Centre for Reproductive Medicine, University Hospital Antwerp, Wilrijkstraat 10, Edegem 2650, Belgium.
authors.forename: Usha
authors.initials: U
authors.lastname: Punjabi
authors.affiliation: Dpto. Reproduccion Animal, Instituto Nacional de Investigación, y Tecnología Agraria y Alimentaria, INIA, Ctra. de la Coruña Km. 5,9, Madrid 28040, Spain.
authors.forename: Priscila
authors.initials: P
authors.lastname: Ramos-Ibeas
authors.affiliation: Dpto. Reproduccion Animal, Instituto Nacional de Investigación, y Tecnología Agraria y Alimentaria, INIA, Ctra. de la Coruña Km. 5,9, Madrid 28040, Spain.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Gutierrez-Adan
authors.affiliation: Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, U0.11, Wilrijk 2610, Belgium.
authors.forename: Peter E
authors.initials: PE
authors.lastname: Bols
authors.affiliation: Veterinary Physiology and Biochemistry, Department of Veterinary Sciences, University of Antwerp, Universiteitsplein 1, Gebouw U, U0.11, Wilrijk 2610, Belgium.
authors.forename: Jo L
authors.initials: JL
authors.lastname: Leroy
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle; D046149:Embryo Culture Techniques / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015571:Follicular Fluid / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D000080864:Lactate Dehydrogenase 5; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D014157:Transcription Factors; C111264:peroxisome-proliferator-activated receptor-gamma coactivator-1; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5
keywords: IVF; embryo quality; follicular fluid; in-vitro maturation; oocyte developmental competence