title: The role of placenta growth factor in the hyperoxia-induced acute lung injury in an animal model.
abstract: Prolonged exposure to hyperoxia leads to acute lung injury. Alveolar type II cells are main target of hyperoxia-induced lung injury. However, the cellular and molecular mechanisms remain unknown. Here, we aimed to investigate the role of placental growth factor (PLGF) in hyperoxia-induced lung injury. Using experimental hyperoxia-induced lung injury model of neonatal rat and mouse lung epithelial type II cells (MLE-12), we examined the levels of PLGF in bronchoalveolar lavage fluid and in the supernatants of MLE-12 cells. Our results revealed that exogenous PLGF induced hyperoxia-induced lung injury. Furthermore, PLGF triggered a shift of vinculin from insoluble to soluble cell fraction, similar to the observation under hyperoxia stimulation. Moreover, we observed significantly reduced phosphorylation of focal adhesion kinase and increased permeability in MLE-12 cells treated with PLGF. These results suggest that PLGF triggers focal adhesion disassembly in alveolar type II cells via inhibiting the activation of focal adhesion kinase. Our findings reveal a novel role of PLGF in hyperoxia-induced lung injury and provide a potential target for the management of hyperoxia-induced acute lung injury.
authors.affiliation: Department of Neonatology, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Yuan
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Shan
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Wu
authors.forename: Xin-Dong
authors.initials: XD
authors.lastname: Xue
mesh_terms: D055371:Acute Lung Injury / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D004847:Epithelial Cells; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism; D018496:Hyperoxia / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010539:Permeability; D000072483:Placenta Growth Factor; D011257:Pregnancy Proteins / Q000378:metabolism*; D051381:Rats
chemical_list: C000608037:Pgf protein, mouse; C000608038:Pgf protein, rat; D011257:Pregnancy Proteins; D000072483:Placenta Growth Factor; D051417:Focal Adhesion Kinase 1; C496051:Ptk2 protein, mouse
keywords: acute lung injury; alveolar type II cells; focal adhesion; focal adhesion kinase; hyperoxia; neonates; placental growth factor