title: Cytidine deaminase motifs within the DYW domain of two pentatricopeptide repeat-containing proteins are required for site-specific chloroplast RNA editing.
abstract: In angiosperm organelles, cytidines are converted to uridines by a deamination reaction in the process termed RNA editing. The C targets of editing are recognized by members of the pentatricopeptide repeat (PPR) protein family. Although other members of the editosome have begun to be identified, the enzyme that catalyzes the C-U conversion is still unknown. The DYW motif at the C terminus of many PPR editing factors contains residues conserved with known cytidine deaminase active sites; however, some PPR editing factors lack a DYW motif. Furthermore, in many PPR-DYW editing factors, the truncation of the DYW motif does not affect editing efficiency, so the role of the DYW motif in RNA editing is unclear. Here, a chloroplast PPR-DYW editing factor, quintuple editing factor 1 (QED1), was shown to affect five different plastid editing sites, the greatest number of chloroplast C targets known to be affected by a single PPR protein. Loss of editing at the five sites resulted in stunted growth and accumulation of apparent photodamage. Adding a C-terminal protein tag to QED1 was found to severely inhibit editing function. QED1 and RARE1, another plastid PPR-DYW editing factor, were discovered to require their DYW motifs for efficient editing. To identify specific residues critical for editing, conserved deaminase residues in each PPR protein were mutagenized. The mutant PPR proteins, when expressed in qed1 or rare1 mutant protoplasts, could not complement the editing defect. Therefore, the DYW motif, and specifically, the deaminase residues, of QED1 and RARE1 are required for editing efficiency.
authors.affiliation: From the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853.
authors.forename: Jessica A
authors.initials: JA
authors.lastname: Wagoner
authors.affiliation: From the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: From the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853.
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.affiliation: From the Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York 14853 mrh5@cornell.edu.
authors.forename: Maureen R
authors.initials: MR
authors.lastname: Hanson
mesh_terms: D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D003564:Cytidine Deaminase / Q000737:chemistry* / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D017434:Protein Structure, Tertiary; D017393:RNA Editing / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D003564:Cytidine Deaminase
keywords: Arabidopsis; Chloroplast; Cytidine Deaminase; PPR Motif; Pentatricopeptide Protein; RNA Editing; Site-directed Mutagenesis; Yeast Two-hybrid Assay