title: High SHIP2 expression indicates poor survival in colorectal cancer.
abstract: SH2-containing inositol 5'-phosphatase 2 (SHIP2), which generally regulates insulin signaling, cytoskeleton remodeling, and receptor endocytosis, has been suggested to play a significant role in tumor development and progression. However, the associations between SHIP2 expression and the clinical features to evaluate its clinicopathologic significance in colorectal cancer (CRC) have not been determined yet. In the present study, one-step quantitative real-time polymerase chain reaction (qPCR) test and immunohistochemistry (IHC) analysis with CRC tissue microarrays (TMA) were employed to evaluate the mRNA and protein expression of SHIP2 in CRC. The results showed that SHIP2 expression in the mRNA and protein levels was significantly higher in CRC tissues than that in corresponding noncancerous tissues (both P < 0.05). The expression of SHIP2 protein in CRC was related to lymph node metastasis (P = 0.036), distant metastasis (P = 0.001), and overall survival (P = 0.009). Kaplan-Meier method and Cox multifactor analysis suggested that high SHIP2 protein level (P = 0.040) and positive distant metastasis (P = 0.048) were critically associated with the unfavorable survival of CRC patients. The findings suggested that SHIP2 may be identified as a useful prognostic marker in CRC and targeting CRC may provide novel strategy for CRC treatment.
authors.affiliation: Department of Gastroenterology, Kunshan Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou 215000, China.
authors.forename: Ju
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Maoying
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Oncology, Kunshan Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou 215000, China.
authors.forename: Yaoguang
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Geriatric Rehabilitation, Kunshan Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Suzhou 215000, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Weng
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute, Nanjing 210000, China.
authors.forename: Weifei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute, Nanjing 210000, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Pu
authors.affiliation: Department of Anesthesiology, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Huihui
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jin
authors.affiliation: Department of Pathology, Nanjing Medical University, Nanjing 210000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Pathology, Jiangsu Province Geriatric Institute, Nanjing 210000, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Jiangsu Province Geriatric Institute, Nanjing 210000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Hematology and Oncology, Jiangsu Province Geriatric Institute, Nanjing 210000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Infectious Diseases, The First People's Hospital of Kunshan Affiliated with Jiangsu University, Suzhou 215000, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Gu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000401:mortality / Q000556:secondary; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics / Q000378:metabolism*; D016016:Proportional Hazards Models
chemical_list: D014408:Biomarkers, Tumor; D010744:Phosphoric Monoester Hydrolases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases