title: Development and validation of RP-HPLC method to determine anti-allergic compound in Thai traditional remedy called Benjalokawichien.
abstract: Benjalokawichien (BLW) or Ya-Ha-Rak (HR) is a traditional remedy in the Nationaldrug list of herbal medicinal products AD 2012 of Thailand. For traditional use, BLW is used as antipyretic agent. It also has anti-allergic effect, particularly treating allergic rash. The ethanolic extract of BLW exhibited anti-allergic activity via inhibitory effect against a release ofbeta-hexosaminidase in RBL-2H3 cell line. Pectolinarigenin has been identified as the active compound ofBLW extract. In this study, a reversed-phase high performance liquid chromatography (RP-HPLC) method was developed in order to control quality ofpreparation in three aspects such as chemical fingerprint, quantification and stability of the ethanolic extract. The RP-HPLC was performed with a gradient mobile phase composed of 0.1% ortho phosphoric acid and acetronitrile, and peaks were detected at 331 nm. Based on validation results, this analytical method is precise, accurate and stable for quantitative determination ofpectolinarigenin. The amount ofpectolinarigenin in Benjalokawichien extract determined by this method was 18.50 mg/g ofextract. Therefore, this method could be consideredfor quality control ofBLWextract.
authors.forename: Intouch
authors.initials: I
authors.lastname: Sakpakdeejaroen
authors.forename: Thana
authors.initials: T
authors.lastname: Juckmeta
authors.forename: Arunporn
authors.initials: A
authors.lastname: Itharat
mesh_terms: D018926:Anti-Allergic Agents / Q000737:chemistry*; D031484:Capparis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D002867:Chromones / Q000737:chemistry; D032409:Clerodendrum / Q000737:chemistry; D030681:Ficus / Q000737:chemistry; D006801:Humans; D029583:Menispermaceae / Q000737:chemistry; D028321:Plant Preparations / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D029641:Simaroubaceae / Q000737:chemistry; D013785:Thailand
chemical_list: D018926:Anti-Allergic Agents; D002867:Chromones; D028321:Plant Preparations; C528671:pectolinarigenin