title: Outcomes of follicle-stimulating hormone priming and nonpriming in in vitro maturation of oocytes in infertile women with polycystic ovarian syndrome: a single-blinded randomized study.
abstract: OBJECTIVE
  Follicle-stimulating hormone (FSH) priming has been studied in in vitro matured oocytes for oocyte maturation rate, embryo quality, and pregnancy rate with discouraging results. This study aimed to initiate FSH stimulation later, i.e. on day 6, to prolong natural endometrial priming and promote oocyte maturation.
  
  
  METHODS
  Forty polycystic ovarian syndrome (PCOS) patients were enrolled into a single, blinded (investigator), randomized, controlled study, and randomly allocated to group 1 (no FSH priming) or group 2 (day 6 recombinant FSH priming). Oocytes were retrieved after human chorionic gonadotropin injection on day 10. After 27 or 51 h of incubation, only mature oocytes were denuded and fertilized by intracytoplasmic sperm injection. Two day 3 embryos were transferred in most patients. Rates of oocyte maturation, cleavage, and pregnancy were compared.
  
  
  RESULTS
  The oocyte maturation rates within 51 h were 62.6 and 72.7% in groups 1 and 2, respectively (p < 0.01). The embryo cleavage rate was significantly higher in group 2 than in group 1 (77.3 vs. 63.6%, p < 0.05). The pregnancy rate was higher in group 1 than in group 2 (50 vs. 30%, p > 0.05).
  
  
  CONCLUSIONS
  FSH priming is beneficial for promotion of the maturation and quality of oocytes, leading to a higher embryo cleavage rate and lower rate of pregnancy loss.
authors.affiliation: Infertility and Reproductive Biology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Roungsin
authors.initials: R
authors.lastname: Choavaratana
authors.forename: Isarin
authors.initials: I
authors.lastname: Thanaboonyawat
authors.forename: Pitak
authors.initials: P
authors.lastname: Laokirkkiat
authors.forename: Japarath
authors.initials: J
authors.lastname: Prechapanich
authors.forename: Singpetch
authors.initials: S
authors.lastname: Suksompong
authors.forename: Orawan
authors.initials: O
authors.lastname: Mekemaharn
authors.forename: Somsin
authors.initials: S
authors.lastname: Petyim
mesh_terms: D000293:Adolescent; D000328:Adult; D006063:Chorionic Gonadotropin / Q000008:administration & dosage / Q000627:therapeutic use; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005640:Follicle Stimulating Hormone / Q000627:therapeutic use*; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D007247:Infertility, Female / Q000188:drug therapy* / Q000628:therapy; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy* / Q000503:physiopathology / Q000628:therapy; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000008:administration & dosage / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D006063:Chorionic Gonadotropin; D011374:Progesterone; D005640:Follicle Stimulating Hormone