title: The effect of chronic peripheral nesfatin-1 application on blood pressure in normal and chronic restraint stressed rats: related with circulating level of blood pressure regulators.
abstract: Nesfatin is a peptide secreted by peripheral tissues, central and peripheral nervous system. It is involved in the regulation of homeostasis. Although the effects of nesfatin-1 on nutrition have been studied widely in the literature, the mechanisms of nesfatin-1 action and also relations with other physiological parameters are still not clarified well. We aimed to investigate the effect of peripheral chronic nesfatin-1 application on blood pressure regulation in normal and in rats exposed to restraint immobilization stress. In our study, three month-old male Wistar rats were used. Rats were divided into 4 groups as Control, Stress, Control+Nesfatin-1, Nesfatin-1+Stress. Angiotensinogen, angiotensin converting enzyme 2, angiotensin II, endothelin-1, endothelial nitric oxide synthase, aldosterone, cortisol, nesfatin-1 levels were determined in plasma samples by ELISA. Our results have shown that chronic peripheral nesfatin-1 administration increases blood pressure in normal and in rats exposed to chronic restraint stress. Effect of nesfatin-1 on circulating level of angiotensinogen, angiotensin converting enzyme 2, angiotensin II, endothelin-1, endothelial nitric oxide synthase, aldosterone and cortisol has been identified. We can conclude that elevated high blood pressure after chronic peripheral nesfatin-1 administration in rats exposed to chronic restraint stress may be related to decreased plasma level of endothelial nitric oxide synthase concentration.
authors.affiliation: Department of Physiology, Faculty of Medicine, University of Dumlupınar, Kütahya, Turkey. ceylanayada@yahoo.com.
authors.forename: Ceylan
authors.initials: C
authors.lastname: Ayada
authors.forename: Günfer
authors.initials: G
authors.lastname: Turgut
authors.forename: Sebahat
authors.initials: S
authors.lastname: Turgut
authors.forename: Zuhal
authors.initials: Z
authors.lastname: Güçlü
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000187:drug effects*; D001835:Body Weight; D002135:Calcium-Binding Proteins / Q000008:administration & dosage*; D004268:DNA-Binding Proteins / Q000008:administration & dosage*; D019332:Endothelin-1 / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D009419:Nerve Tissue Proteins / Q000008:administration & dosage*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D000080866:Nucleobindins; D051381:Rats; D017208:Rats, Wistar; D012084:Renin-Angiotensin System; D013312:Stress, Physiological
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D019332:Endothelin-1; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat