title: Effect of perfluorooctane sulfonate on pluripotency and differentiation factors in mouse embryoid bodies.
abstract: Perfluorooctane sulfonate (PFOS) poses potential risks to early development, but the molecular mechanisms how PFOS affects embryonic development are still unclear. Mouse embryoid bodies (mEBs) provide ideal models for testing safety or toxicity of chemicals in vitro. In this study, mEBs were exposed to PFOS up to 6 days and then their pluripotency and differentiation markers were evaluated. Our data showed that the mRNA and protein levels of pluripotency markers (Oct4, Sox2, Nanog) in mEBs were significantly increased following exposure to PFOS. Meanwhile, the expressions of miR-134, miR-145, miR-490-3p were decreased accordingly. PFOS reduced the mRNA levels of endodermal markers (Sox17, FOXA2), mesodermal markers (SMA, Brachyury) and ectodermal markers (Nestin, Fgf5) in mEBs. Meanwhile, PFOS increased the mRNA and protein levels of polycomb group (PcG) family members (Cbx4, Cbx7, Ezh2). Overall, our results showed that PFOS could increase the expression levels of pluripotency factors and decrease the differentiation markers.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Department of Endocrinology, The Affiliated Jiangyin People's Hospital of Wuxi Clinical School of Medicine, Nanjing Medical University, Jiangyin 214400, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Hospital, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiaojiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Han
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, The Affiliated Jiangyin People's Hospital of Wuxi Clinical School of Medicine, Nanjing Medical University, Jiangyin 214400, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China. Electronic address: yankaixia@njmu.edu.cn.
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D019070:Cell Lineage; D058732:Embryoid Bodies / Q000187:drug effects* / Q000378:metabolism; D005466:Fluorocarbons / Q000633:toxicity*; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000187:drug effects* / Q000378:metabolism; D063146:Polycomb-Group Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
chemical_list: D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D005819:Genetic Markers; D035683:MicroRNAs; D063146:Polycomb-Group Proteins; D012333:RNA, Messenger; C076994:perfluorooctane sulfonic acid
keywords: Differentiation; MicroRNA; Mouse embryoid bodies; Perfluorooctane sulfonate; Pluripotency; Polycomb group