title: Evaluation of the Cepheid Xpert C. difficile/Epi and meridian bioscience illumigene C. difficile assays for detecting Clostridium difficile ribotype 033 strains.
abstract: Clostridium difficile PCR ribotype 033 (RT033) is found in the gastrointestinal tracts of production animals and, occasionally, humans. The illumigene C. difficile assay (Meridian Bioscience, Inc.) failed to detect any of 52 C. difficile RT033 isolates, while all strains signaled positive for the binary toxin genes but were reported as negative for C. difficile by the Xpert C. difficile/Epi assay (Cepheid).
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Grace O
authors.initials: GO
authors.lastname: Androga
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Alan M
authors.initials: AM
authors.lastname: McGovern
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Briony
authors.initials: B
authors.lastname: Elliott
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Chang
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Timothy T
authors.initials: TT
authors.lastname: Perkins
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia.
authors.forename: Niki F
authors.initials: NF
authors.lastname: Foster
authors.affiliation: School of Pathology and Laboratory Medicine, The University of Western Australia, Crawley, Western Australia, Australia Department of Microbiology, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands, Western Australia, Australia thomas.riley@uwa.edu.au.
authors.forename: Thomas V
authors.initials: TV
authors.lastname: Riley
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D016360:Clostridioides difficile / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003015:Clostridium Infections / Q000175:diagnosis* / Q000382:microbiology* / Q000662:veterinary; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021521:Ribotyping; D012680:Sensitivity and Specificity