title: Hypoglycemic and hypolipidemic properties of polysaccharides from Enterobacter cloacae Z0206 in KKAy mice.
abstract: The water-soluble polysaccharides (EPS) were isolated from culture broth of Enterobacter cloacae Z0206, and the hypoglycemic and hypolipidemic effects of EPS were investigated. In this study, KKAy mice were gavaged once daily with either distilled water or EPS (200 mg/kg body weight) for 6 weeks. Results showed that EPS possessed significant hypoglycemic effects. Improved oral glucose tolerance, reduced serum insulin levels as well as decreased serum triglycerides (TG), cholesterol (TC) and low density lipoprotein cholesterol (LDL-c) were observed after treatment with EPS. Furthermore, EPS upregulated the expression of glucokinase (GK), HSL (Hormone Sensitive Lipase), adipose triglyceride lipase (ATGL), carnitine palmitoyl transferase 1-α (CPT1-α), glucose transporter 2 (Glut2), adenosine monophosphate activated protein kinase (AMPK) and silent information regulator 1 (Sirt1), but downregulated the gene expression of glucose-6-phosphatase (G6P) and fatty acid synthase (FAS) in the liver. These results suggest that EPS exhibits hypoglycemic and hypolipidemic effects possibly through regulating AMPK- and SirT1-mediated effects on carbohydrate and lipid metabolism.
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Huang
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China.
authors.forename: Hangxian
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou 310058, PR China. Electronic address: yzwang@zju.edu.cn.
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D016972:Enterobacter cloacae / Q000737:chemistry*; D005215:Fasting / Q000097:blood; D005951:Glucose Tolerance Test; D006003:Glycogen / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D000960:Hypolipidemic Agents; D007328:Insulin; D008055:Lipids; D011134:Polysaccharides; D006003:Glycogen; D006593:Hexokinase
keywords: Hypoglycemic; KKAy mice; Polysaccharide; Type 2 diabetes