title: Gene-ectomy: gene ablation with CRISPR/Cas9 in human hematopoietic cells.
abstract: CRISPR/Cas9 has recently been introduced as a gene editing tool and shows considerable promise. In this issue of Cell Stem Cell, Mandal et al. (2014) show efficient CRISPR/Cas9-mediated ablation of the CCR5 and B2M genes in primary human hematopoietic cells, two editing strategies that are potentially translatable into clinical application.
authors.affiliation: Infection, Immunity, Inflammation and Physiological Medicine Programme, Molecular and Cellular Immunology Section, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK.
authors.forename: Miguel
authors.initials: M
authors.lastname: Calero-Garcia
authors.affiliation: Infection, Immunity, Inflammation and Physiological Medicine Programme, Molecular and Cellular Immunology Section, UCL Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK. Electronic address: h.gaspar@ucl.ac.uk.
authors.forename: H Bobby
authors.initials: HB
authors.lastname: Gaspar
mesh_terms: D000818:Animals; D064130:CRISPR-Associated Proteins / Q000378:metabolism*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D017353:Gene Deletion; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans
chemical_list: D064130:CRISPR-Associated Proteins