title: CuO-induced signal amplification strategy for multiplexed photoelectrochemical immunosensing using CdS sensitized ZnO nanotubes arrays as photoactive material and AuPd alloy nanoparticles as electron sink.
abstract: In this work, multiplexed photoelectrochemical (PEC) immunoassays are introduced into an indium tin oxide (ITO) device. Firstly, the ITO device is fabricated using a simple acid etch treatment method. Secondly, AuPd alloy nanoparticles are electro-deposited on ITO working electrodes as electron sink to construct the immunosensor platform. After that, ZnO nanotubes (ZNTs) arrays are synthesized via chemical etching of ZnO nanorods that are grown on AuPd surface by electrochemical deposition method. Subsequently, CdS is electro-deposited on ZNTs arrays and used as photoactive material. Then, CuO nanoseeds are labeled with signal antibodies and firstly used as PEC signal amplification label. The introduction of CuO brings signal amplification because of the conduction band (CB) of both CuO and ZnO are lower than that of CdS, CuO will compete the photo-induced electrons in CB of CdS with ZnO, leading to the decrease of the photocurrent intensity. Using cancer antigen 125, prostate specific antigen and α-fetoprotein as model analytes, the proposed immunoassay exhibits excellent precision and sensitivity. Meanwhile, this work provides a promising, addressable and simple strategy for the multi-detection of tumor markers.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Qingkun
authors.initials: Q
authors.lastname: Kong
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: ujn.yujh@gmail.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
authors.affiliation: Cancer Research Center, Shandong Tumor Hospital, Jinan 250117, PR China.
authors.forename: Xianrang
authors.initials: X
authors.lastname: Song
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D015374:Biosensing Techniques / Q000379:methods* / Q000706:statistics & numerical data; D018394:CA-125 Antigen / Q000032:analysis; D019187:Cadmium Compounds; D003300:Copper; D003429:Cross Reactions; D055664:Electrochemical Techniques; D004583:Electrons; D006047:Gold Alloys; D006801:Humans; D007118:Immunoassay / Q000379:methods* / Q000706:statistics & numerical data; D007610:Kallikreins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D053768:Metal Nanoparticles / Q000648:ultrastructure; D043942:Nanotubes / Q000648:ultrastructure; D010165:Palladium; D017430:Prostate-Specific Antigen / Q000032:analysis; D015203:Reproducibility of Results; D013440:Sulfides; D017971:Tin Compounds; D015034:Zinc Oxide; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: C097353:AFP protein, human; D014408:Biomarkers, Tumor; D018394:CA-125 Antigen; D019187:Cadmium Compounds; D006047:Gold Alloys; C451759:MUC16 protein, human; D008565:Membrane Proteins; D013440:Sulfides; D017971:Tin Compounds; D000509:alpha-Fetoproteins; C034939:cadmium sulfide; D010165:Palladium; C109984:indium tin oxide; D003300:Copper; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen; D015034:Zinc Oxide; C030973:cupric oxide
keywords: Electron sink; Indium tin oxide; Multiplexed immunoassays; Photoelectrochemical; Signal amplification.