title: Versatile microfluidic droplets array for bioanalysis.
abstract: We propose a novel method to obtain versatile droplets arrays on a regional hydrophilic chip that is fabricated by PDMS soft lithography and regional plasma treatment. It enables rapid liquid dispensation and droplets array formation just making the chip surface in contact with solution. By combining this chip with a special Christmas Tree structure, the droplets array with concentrations in gradient is generated. It possesses the greatly improved performance of convenience and versatility in bioscreening and biosensing. For example, high throughput condition screening of toxic tests of CdSe quantum dots on HL-60 cells are conducted and cell death rates are successfully counted quickly and efficiently. Furthermore, a rapid biosensing approach for cancer biomarkers carcinoma embryonic antigen (CEA) is developed via magnetic beads (MBs)-based sandwich immunoassay methods.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, P.R. China.
authors.forename: Shan-Wen
authors.initials: SW
authors.lastname: Hu
authors.forename: Bi-Yi
authors.initials: BY
authors.lastname: Xu
authors.forename: Wei-Ke
authors.initials: WK
authors.lastname: Ye
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Xia
authors.forename: Hong-Yuan
authors.initials: HY
authors.lastname: Chen
authors.forename: Jing-Juan
authors.initials: JJ
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000737:chemistry; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D020724:Elastomers / Q000737:chemistry; D018922:HL-60 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007118:Immunoassay; D008280:Magnetics; D008422:Materials Testing; D046210:Microfluidic Analytical Techniques; D044085:Microfluidics / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry; D013499:Surface Properties; D017155:Wettability
chemical_list: D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D004129:Dimethylpolysiloxanes; D020724:Elastomers; D018036:Selenium Compounds; C013830:baysilon; C058667:cadmium selenide
keywords: droplets array; liquid dispensation; rapid analysis; surface wettability