title: Bacillus subtilis biosensor engineered to assess meat spoilage.
abstract: Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage.
authors.affiliation: iGEM Teaching Program, Team 2012, ‡Molecular Genetics Group, and §Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen , 9747 AG Groningen, The Netherlands.
authors.forename: Alicja
authors.initials: A
authors.lastname: Daszczuk
authors.forename: Yonathan
authors.initials: Y
authors.lastname: Dessalegne
authors.forename: Ismaêl
authors.initials: I
authors.lastname: Drenth
authors.forename: Elbrich
authors.initials: E
authors.lastname: Hendriks
authors.forename: Emeraldo
authors.initials: E
authors.lastname: Jo
authors.forename: Tom
authors.initials: T
authors.lastname: van Lente
authors.forename: Arjan
authors.initials: A
authors.lastname: Oldebesten
authors.forename: Jonathon
authors.initials: J
authors.lastname: Parrish
authors.forename: Wlada
authors.initials: W
authors.lastname: Poljakova
authors.forename: Annisa A
authors.initials: AA
authors.lastname: Purwanto
authors.forename: Renske
authors.initials: R
authors.lastname: van Raaphorst
authors.forename: Mirjam
authors.initials: M
authors.lastname: Boonstra
authors.forename: Auke
authors.initials: A
authors.lastname: van Heel
authors.forename: Martijn
authors.initials: M
authors.lastname: Herber
authors.forename: Sjoerd
authors.initials: S
authors.lastname: van der Meulen
authors.forename: Jeroen
authors.initials: J
authors.lastname: Siebring
authors.forename: Robin A
authors.initials: RA
authors.lastname: Sorg
authors.forename: Matthias
authors.initials: M
authors.lastname: Heinemann
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: Veening
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes; D020869:Gene Expression Profiling; D005818:Genetic Engineering / Q000379:methods*; D008460:Meat / Q000032:analysis*; D010456:Peptides, Cyclic / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D058615:Synthetic Biology; D055549:Volatile Organic Compounds
chemical_list: D001430:Bacteriocins; D005456:Fluorescent Dyes; D010456:Peptides, Cyclic; D055549:Volatile Organic Compounds; C047828:sboA protein, Bacillus subtilis