title: DODAB:monoolein liposomes containing Candida albicans cell wall surface proteins: a novel adjuvant and delivery system.
abstract: We describe the preparation and characterization of DODAB:MO-based liposomes and demonstrate their adjuvant potential and use in antigen delivery. Liposomes loaded with Candida albicans proteins assembled as stable negatively charged spherical nanoparticles with a mean size of 280 nm. High adsorption efficiency (91.0 ± 9.0%) is attained with high lipid concentrations. The nanoparticles were non-toxic, avidly taken up by macrophage cells and accumulated in membrane rich regions with an internalization time of 20 min. Immunized mice displayed strong humoral and cell-mediated immune responses, producing antibodies (IgGs) against specific cell wall proteins, Cht3p and Xog1p. DODAB:MO-based liposomes loaded with C. albicans proteins have an excellent immunogenic potential and can be explored for the development of an immunoprotective strategy against Candida infections.
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
authors.forename: Catarina
authors.initials: C
authors.lastname: Carneiro
authors.affiliation: IBMC - Instituto de Biologia Molecular e Celular, Porto, Portugal.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Correia
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
authors.forename: Tony
authors.initials: T
authors.lastname: Collins
authors.affiliation: IBMC - Instituto de Biologia Molecular e Celular, Porto, Portugal; Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Porto, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Vilanova
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
authors.forename: Célia
authors.initials: C
authors.lastname: Pais
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal; NanoDelivery I&D in Biotechnology, Biology Department, Braga, Portugal.
authors.forename: Andreia C
authors.initials: AC
authors.lastname: Gomes
authors.affiliation: Centre of Physics (CFUM), University of Minho, Braga, Portugal; NanoDelivery I&D in Biotechnology, Biology Department, Braga, Portugal.
authors.forename: M Elisabete C D
authors.initials: ME
authors.lastname: Real Oliveira
authors.affiliation: Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal. Electronic address: psampaio@bio.uminho.
authors.forename: Paula
authors.initials: P
authors.lastname: Sampaio
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D002176:Candida albicans / Q000276:immunology*; D002460:Cell Line; D002473:Cell Wall / Q000276:immunology*; D016503:Drug Delivery Systems / Q000379:methods; D005989:Glycerides / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage; D010316:Particle Size; D000644:Quaternary Ammonium Compounds / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D000941:Antigens; D005989:Glycerides; D007074:Immunoglobulin G; D008081:Liposomes; D008565:Membrane Proteins; D000644:Quaternary Ammonium Compounds; C015831:dimethyldioctadecylammonium; C005953:monoolein
keywords: Adjuvant activity; Antigen delivery systems; Antigen protection; Candida albicans proteins; DODAB:MO liposomes; Humoral and cellular immune responses; Liposome endocytosis; Nanoparticles