title: Development of a Selective Medium for the Fungal Pathogen Cylindrocarpon destructans Using Radicicol.
abstract: The soil-borne ascomycete fungus Cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin A and radicicol. The slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. In this study, we developed a selective medium for C. destructans using radicicol produced by this fungus. Supplementing 50 mg/L of radicicol to medium inhibited the mycelia growth of other fungi including Botrytis cinerea, Rhizoctonia solani and Alternaria panax, but did not affect the growth of C. destructans. In addition, conidia germination of other fungal species except for C. destructans was inhibited in submerged culture supplemented with radicicol. This medium provides a very efficient tool for isolating C. destructans and also can be used as an enrichment medium for this fungus.
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Yunhee
authors.initials: Y
authors.lastname: Kang
authors.affiliation: Ginseng Research Division, National Institute of Horticultural and Herbal Science, Eumseong 369-873, Korea.
authors.forename: Seung-Ho
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Applied Biology, Dong-A University, Busan 604-714, Korea.
authors.forename: Jungkwan
authors.initials: J
authors.lastname: Lee
keywords: Cylindrocarpon destructans; Fusarium solani; ginseng root rot; radicicol; selective medium