title: Generation and application of polyclonal IgY antibodies specific for full-length and nicked prostate-specific antigen.
abstract: BACKGROUND
  The prostate-specific antigen (PSA) is considered an important serum marker for prostate cancer detection, monitoring and staging. The purpose of this study was to generate IgY class antibodies that recognize native PSA and selected epitopes.
  
  
  METHODS
  Hens immunized with either full-length human PSA or its peptidyl fragment-conjugates produced specific antibodies that were isolated from egg yolks. We developed a monoclonal/IgY sandwich ELISA with a PSA detection limit of 50 pg/ml and a linear range of 0.05-1.0 ng/ml.
  
  
  CONCLUSIONS
  Because the signal observed for the PSA-specific IgY antibodies by ELISA and the reactivity profile of the epitope-derived IgYs were comparable to those of mouse monoclonal IgG antibodies, avian antibodies may be a cost-effective alternative to mammalian antibodies for prostate cancer diagnostics.
authors.affiliation: Division of Medicinal Chemistry & Microbiology, Faculty of Chemistry, Wroclaw University of Technology, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Łupicka-Słowik
authors.forename: Maciej
authors.initials: M
authors.lastname: Walczak
authors.forename: Renata
authors.initials: R
authors.lastname: Grzywa
authors.forename: Kamila
authors.initials: K
authors.lastname: Bobrek
authors.forename: Maria
authors.initials: M
authors.lastname: Łęcka
authors.forename: Stephane
authors.initials: S
authors.lastname: Boivin
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gaweł
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Stefaniak
authors.forename: Józef
authors.initials: J
authors.lastname: Oleksyszyn
authors.forename: Marcin
authors.initials: M
authors.lastname: Sieńczyk
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D007114:Immunization; D007136:Immunoglobulins / Q000276:immunology* / Q000302:isolation & purification; D007610:Kallikreins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D057230:Limit of Detection; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology*; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000737:chemistry* / Q000276:immunology*; D015203:Reproducibility of Results
chemical_list: D000939:Epitopes; C016512:IgY; D007136:Immunoglobulins; D010446:Peptide Fragments; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen