title: Determination by GC×GC of fatty acid and conjugated linoleic acid (CLA) isomer profiles in six selected tissues of lambs fed on pasture or on indoor diets with and without rumen-protected CLA.
abstract: In this study GC×GC was used to study the effects of pasture, hay, concentrate (indoor), and indoor plus 8 g/day of a rumen-protected conjugated linoleic acid (indoor-CLA) diets on the detailed fatty acid (FA) profiles of six tissues (muscles, fatty tissues, and liver) collected from 36 lambs. This powerful technique allowed the quantification of 128 FAs, of which 21 SFAs, 16 MUFAs, 19 PUFAs were identified by reference standards. The diets had similar, but not identical, effects on FA profiles (g/100 g FA) in the various tissues, as both indoor diets reduced total PUFAs (from 8.91 ± 6.27 to 8.06 ± 5.97; p < 0.05) and n-3 PUFAs (from 2.70 ± 2.37 to 1.50 ± 1.69; p < 0.01) and increased n-6 PUFA (from 3.76 ± 2.46 to 4.58 ± 3.42; p < 0.01), branched (from 2.37 ± 2.05 to 3.23 ± 0.54; p < 0.01), odd-chain FAs (from 5.88 ± 5.33 to 7.07 ± 1.51; p < 0.01) compared to pasture. Indoor-CLA increased CLAc9,t11 (from 0.42 ± 0.13 to 0.53 ± 0.19; p < 0.01), CLAt10,c12 (from 0.07 ± 0.06 to 0.12 ± 0.22; p < 0.05), and CLAc11,t13 (from 0.02 ± 0.04 to 0.05 ± 0.04; p < 0.05) compared to indoor.
authors.affiliation: Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova , Viale dell'Università 16, 35020 Legnaro (PD), Italy.
authors.forename: Erika
authors.initials: E
authors.lastname: Pellattiero
authors.forename: Alessio
authors.initials: A
authors.lastname: Cecchinato
authors.forename: Franco
authors.initials: F
authors.lastname: Tagliapietra
authors.forename: Stefano
authors.initials: S
authors.lastname: Schiavon
authors.forename: Giovanni
authors.initials: G
authors.lastname: Bittante
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000821:Animal Feed; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D004032:Diet / Q000662:veterinary*; D004041:Dietary Fats / Q000008:administration & dosage; D005227:Fatty Acids / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage; D044243:Linoleic Acids, Conjugated / Q000008:administration & dosage / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D012417:Rumen / Q000378:metabolism; D012756:Sheep
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D044243:Linoleic Acids, Conjugated
keywords: fatty acids; lambs; rumen-protected conjugated linoleic acid; tissues; two-dimensional gas chromatography