title: Drug resistance effects of ribosomal protein L24 overexpression in hepatocellular carcinoma HepG2 cells.
abstract: BACKGROUND
  The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer.
  
  
  METHODS
  Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null- transfected HepG2 cell lines.
  
  
  RESULTS
  The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation.
  
  
  CONCLUSIONS
  The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.
authors.affiliation: Oncology Department of the Jining First People's Hospital, Jining, Shandong, People's Republic of China E-mail : 1392144@163.com.
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Guo
authors.forename: Qing-Sheng
authors.initials: QS
authors.lastname: Kong
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Liu
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Tan
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D049109:Cell Proliferation / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006146:Guanidines / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006146:Guanidines; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C036298:ribosomal protein L24; C065212:amycin A