title: Silencing of polo-like kinase 2 increases cell proliferation and decreases apoptosis in SGC-7901 gastric cancer cells.
abstract: Polo‑like kinase 2 (PLK2) is a serine/threonine protein kinase, which has vital roles during mitosis and the centrosome cycle. In acute myeloblastic leukemia and hepatocarcinogenesis, PLK2 acts as a tumor suppressor; however, the function of PLK2 in gastric cancer remains to be elucidated. In the present study, PLK2 was overexpressed in gastric cancer tissues and three types of gastric cancer cells, SGC‑7901, MKN‑45 and BGC‑823. Transfection of SGC‑7901 gastric cancer cells with small interfering (si)RNA against PLK2 exerted no effect on the ratio of cells at different stages of the cell cycle compared with that of the untransfected and control siRNA‑transfected cells. In addition, silencing of PLK2 significantly enhanced the growth of SGC‑7901 cells through inhibiting apoptosis. Furthermore, apoptosis‑associated genes Bax and caspase 3 were found to be downregulated at the protein level. In conclusion, these results suggested that PLK2 may act as a tumor suppressor in gastric cancer, therefore indicating its therapeutic potential.
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Li Ying
authors.initials: LY
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ling Yu
authors.initials: LY
authors.lastname: Zhao
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Xiao Ge
authors.initials: XG
authors.lastname: Zhao
authors.affiliation: Department of Genetics and Molecular Biology, Molecular Bacteriology Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Tu Sheng
authors.initials: TS
authors.lastname: Song
authors.affiliation: Center Laboratory of Biomedical Research, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Immunology and Pathogenic Biology, Molecular Bacteriology Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Chinese Ministry of Education, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, P.R. China.
authors.forename: Ji Ru
authors.initials: JR
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D051017:Apoptosis Regulatory Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C496126:PLK2 protein, human; D017346:Protein Serine-Threonine Kinases