title: Therapeutic efficacy of autologous platelet-rich plasma and polydeoxyribonucleotide on female pattern hair loss.
abstract: Autologous platelet-rich plasma (PRP) exerts positive therapeutic effects on hair thickness and density in patients with pattern hair loss. The aim of our study was to evaluate the efficacy of intra-perifollicular autologous PRP and polydeoxyribonucleotide (PDRN) injections in treating female pattern hair loss (FPHL). Twenty FPHL patients were treated with a single session of PRP injection, followed by 12 sessions of PDRN intra-perifollicular injection, along the scalp at weekly intervals. Additionally, another 20 FPHL patients were treated with 12 sessions of PDRN injection only. Meanwhile, one half of the backs of two rabbits was injected with the PRP preparation, while the other half was injected with phosphate buffered saline as a control. Tissue samples from the rabbits were analyzed by real-time polymerase chain reaction and Western blotting. Compared with baseline values, patients treated with PRP and PDRN injections exhibited clinical improvement in mean hair counts (23.2 ± 15.5%; p < 0.001) and mean hair thickness (16.8 ± 10.8%; p < 0.001). In addition, patients treated with the 12 sessions of intra-perifollicular PDRN injection alone also showed clinical improvement in mean hair counts (17.9 ± 13.2%; p < 0.001) and mean hair thickness (13.5 ± 10.7%; p < 0.001). Comparison analyses between the two groups revealed that combined therapy with PRP and PDRN induces greater improvement in hair thickness than treatment with PDRN therapy alone (p = 0.031), but not in hair counts (p > 0.05). The pilot animal study revealed significant up-regulation of WNT, platelet-derived growth factor, and fibroblast growth factor expression in rabbit skin treated with the PRP preparation, compared with control skin. In conclusion, intra-perifollicular injections of autologous PRP and/or PDRN generate improvements in hair thickness and density in FPHL patients.
authors.affiliation: Laboratory of Disease Functional Genomics, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea.
authors.forename: Si-Hyung
authors.initials: SH
authors.lastname: Lee
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Jin-Soo
authors.initials: JS
authors.lastname: Kang
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Kim
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Oh
authors.forename: Sung Bin
authors.initials: SB
authors.lastname: Cho
mesh_terms: D000328:Adult; D000505:Alopecia / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006197:Hair / Q000187:drug effects / Q000254:growth & development*; D018859:Hair Follicle / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D007267:Injections; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000008:administration & dosage* / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D011817:Rabbits; D044968:Regenerative Medicine; D056910:Republic of Korea; D012535:Scalp / Q000187:drug effects / Q000254:growth & development* / Q000473:pathology; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism; D000071818:Wnt-5a Protein
chemical_list: D011089:Polydeoxyribonucleotides; D011518:Proto-Oncogene Proteins; C484142:WNT10A protein, human; C085389:WNT5A protein, human; D051153:Wnt Proteins; D000071818:Wnt-5a Protein