title: Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio.
abstract: Titanium dioxide nanoparticles (TiO2 NPs), widely used in paints, pharmaceutical preparations and in many consumer products, have been shown to induce cytotoxicity, genotoxicity and carcinogenic responses both in vitro and in vivo. Numerous studies have shown the potential impact of nanoparticles on a series of aquatic organisms and their toxicity has been linked to their dissolution, surface properties and size. In vitro studies have raised concerns about the toxicity of TiO2 NPs, but there are very limited data on ecotoxicity to aquatic life. This in vivo study aimed to describe the genotoxicity of TiO2 NPs in the zebrafish Danio rerio. After 2 weeks of adaptation, groups of zebrafish were exposed to TiO2 NPs (1 and 10μg/L) for 5, 7, 14, 21 and 28 days. The genotoxic potential of TiO2 NPs was assessed by the Comet assay, the Diffusion assay and RAPD-PCR technique. The use of multi-biomarkers has become an important aspect of ecotoxicology to evaluate environmental quality through a wide panel of biological responses triggered by contaminants. The highest genotoxic effect was observed at the maximum concentrations of nanoparticles (10μg/L) with all three tests at 14 and 21 days of exposure. The results suggests the presence of mechanisms that can reduce the n-TiO2 genotoxicity. Future studies are necessary to analyze the DNA repairing capacity in zebrafish cells and so verify the role of the antioxidant defence system in modulating the response to exposure to n-TiO2 in fish.
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy. Electronic address: lucia.rocco@unina2.it.
authors.forename: Lucia
authors.initials: L
authors.lastname: Rocco
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Marianna
authors.initials: M
authors.lastname: Santonastaso
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Filomena
authors.initials: F
authors.lastname: Mottola
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Costagliola
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Suero
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Severina
authors.initials: S
authors.lastname: Pacifico
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Via Vivaldi 43, Caserta, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Stingo
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D053758:Nanoparticles / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique; D014025:Titanium / Q000633:toxicity*; D015027:Zebrafish
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: Comet assay; DNA damage; Diffusion assay; Genomic template stability; RAPD-PCR technique; Zebrafish