title: Fluoroquinolone and Ovariectomy in the Bitch: Physiology of the Immune System as to CD56 and CD16 Expression.
abstract: Natural killer (NK) cells are innate lymphoid cells which act against a variety of pathogens and tumours. Phenotypically they are characterized by surface markers named cluster designation (CD) antigens. CD56 and CD16 are recognized as specific NK markers in the dogs as well as in humans. Surgical interventions suppress NK cells both in rats and humans. In this direction, it has been shown that an antibiotic regimen (amoxicillin, benzylpenicillin/dihydrostreptomycin, sulfametazine/sulfamerazine/ sulfathiazole, enrofloxacin, lincomycin/spectinomycin) administered only twice is effective in preventing infections after laparatomic ovariectomy, in the bitch. On these grounds, this research will show that the administration of a fluoroquinolone (5 mg/kg of enrofloxacin, Baytril®, Bayer, Milan, Italy) one hour before and at the end of ovariectomy is able to increase CD56 and CD16 expression levels. Moreover, the antibiotic administration modifies the relative expression levels of the two CD; thus suggesting that the fluoroquinolone employed enhances the activation of a specific subset of NK cells mainly involved in body recovering during the post operative period as already observed in humans.
authors.forename: Maria
authors.initials: M
authors.lastname: Albrizio
authors.forename: Annalisa
authors.initials: A
authors.lastname: Rizzo
authors.forename: Marianna
authors.initials: M
authors.lastname: Pantaleo
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Pampurini
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Piccinno
authors.affiliation: Department of the Emergency and Organ Transplantation (D.E.T.O.) - Section of Veterinary Clinics and Animal Production, University of Bari "Aldo Moro", Strada prov.le per Casamassima km3 - 70010, Valenzano (BA), Italy. raffaeleluigi.sciorsci@uniba.it.
authors.forename: Raffaele Luigi
authors.initials: RL
authors.lastname: Sciorsci
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015153:Blotting, Western; D019002:CD56 Antigen / Q000378:metabolism*; D004285:Dogs; D000077422:Enrofloxacin; D005260:Female; D005455:Fluorescent Antibody Technique; D024841:Fluoroquinolones / Q000494:pharmacology*; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D010052:Ovariectomy; D017452:Receptors, IgG / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D019002:CD56 Antigen; C428200:FCGR3B protein, human; D024841:Fluoroquinolones; D058851:GPI-Linked Proteins; C522918:NCAM1 protein, human; D017452:Receptors, IgG; D000077422:Enrofloxacin