title: Acanthamoeba encephalitis: isolation of genotype T1 in mycobacterial liquid culture medium.
abstract: We report a case of Acanthamoeba encephalitis diagnosed from an antemortem brain biopsy specimen, where the organism was first isolated in mycobacterial liquid medium and first identified by using a sequence generated by a commercial panfungal sequencing assay. We correlate susceptibility results with clinical outcome.
authors.affiliation: Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia.
authors.forename: Rula
authors.initials: R
authors.lastname: Azzam
authors.affiliation: Department of Ophthalmology, Flinders Medical Centre and Flinders University, Bedford Park, South Australia, Australia.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Badenoch
authors.affiliation: Department of Microbiology, Monash Health, Clayton, Victoria, Australia.
authors.forename: Michelle J
authors.initials: MJ
authors.lastname: Francis
authors.affiliation: Department of Microbiology, Monash Health, Clayton, Victoria, Australia.
authors.forename: Charles
authors.initials: C
authors.lastname: Fernandez
authors.affiliation: Department of Microbiology and Infectious Diseases, SA Pathology at Flinders Medical Centre and Flinders University, Bedford Park, South Australia.
authors.forename: Penelope J
authors.initials: PJ
authors.lastname: Adamson
authors.affiliation: Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Medicine, Monash University, Clayton, Victoria, Australia.
authors.forename: Claire
authors.initials: C
authors.lastname: Dendle
authors.affiliation: Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Medicine, Monash University, Clayton, Victoria, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Woolley
authors.affiliation: Sullivan Nicolaides Pathology, Brisbane, Queensland, Australia.
authors.forename: Jenny
authors.initials: J
authors.lastname: Robson
authors.affiliation: Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Microbiology, Monash Health, Clayton, Victoria, Australia Department of Medicine, Monash University, Clayton, Victoria, Australia.
authors.forename: Tony M
authors.initials: TM
authors.lastname: Korman
authors.affiliation: Monash Infectious Diseases, Monash Health, Clayton, Victoria, Australia Department of Microbiology, Monash Health, Clayton, Victoria, Australia Maryza.Graham@monashhealth.org.
authors.forename: Maryza
authors.initials: M
authors.lastname: Graham
mesh_terms: D000048:Acanthamoeba / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D000368:Aged; D001706:Biopsy; D001921:Brain / Q000000981:diagnostic imaging / Q000469:parasitology* / Q000473:pathology; D020808:Central Nervous System Protozoal Infections / Q000175:diagnosis* / Q000469:parasitology; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005838:Genotype; D006651:Histocytochemistry; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008828:Microbiological Techniques; D008853:Microscopy; D008969:Molecular Sequence Data; D011859:Radiography; D017422:Sequence Analysis, DNA
chemical_list: D016054:DNA, Protozoan