title: [Embryotoxicity of Senecionis Scandentis Hebra on in vitro cultured mouse embryos].
abstract: The purpose of this study is to evaluate the embryotoxicity of alkaloids in Senecionis Scandentis Hebra on in vitro cultured mouse embryos. Mouse whole embryo culture (WEC) was applied in this study. Post-implantation (8.5 d) mouse embryos were isolated from their mothers, and cultured in medium of immediately centrifuged serum (ICS) with different concentrations of seneciphylline (target concentrations were 100, 50, 25 and 12.5 μg x mL(-1)) or senkirkine (target concentrations were 50, 25 and 12.5 μg x mL(-1)) for 48 h. After culturing completed, the development and organic morphodifferentiation of the cultured embryos were evaluated microscopically. Treatment with seneciphylline and senkirkine had adverse effects on the development and organic morphodifferentiation of embryos. The effect also had clear dose-response. Alkaloidals in Senecionis Scandentis Hebra had embryotoxicity on cultured embryos, which indicated that pregnant people exposed to Senecionis Scandentis Hebra may get potential risk on fetus.
authors.forename: Jia-Yin
authors.initials: JY
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Yi
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Liang
authors.forename: Yu-Shi
authors.initials: YS
authors.lastname: Zhang
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Cui
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Lu
mesh_terms: D000818:Animals; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects*; D005260:Female; D051379:Mice; D011247:Pregnancy; D011763:Pyrrolizidine Alkaloids / Q000633:toxicity*; D012674:Senecio / Q000737:chemistry*; D013723:Teratogens / Q000633:toxicity*
chemical_list: D011763:Pyrrolizidine Alkaloids; D013723:Teratogens; C013678:seneciphylline; C009238:senkirkine