title: Effects of glucose and sucrose variants of the caries-promoting Diet 2000 on the feeding patterns and parotid glands of prematurely weaned rats.
abstract: OBJECTIVE
  The hypothesis of this study was that feeding glucose instead of sucrose in the cariogenic Diet 2000 to rats weaned at age 18 days would result in greater light/dark differences in feeding activity and secretion and storage of parotid salivary enzymes.
  
  
  METHODS
  Diet 2000 and a stock commercial diet (controls) were prepared in pelleted and powdered forms, as the increased mastication required by pellets has been shown to support circadian rhythms in rats. Food jars were weighed at lights on and just prior to lights off daily. Rats were euthanized at 25 days and their parotid glands removed, weighed, and analyzed for specific activities of the salivary enzymes α-amylase and deoxyribonuclease I.
  
  
  RESULTS
  Light/dark differences in feeding activity were strong in the rats fed the pelleted stock diet and both powdered and pelleted glucose 2000 diets, moderate with the pelleted sucrose 2000 diet, and not significant with the powdered sucrose 2000 and stock diets. Light/dark differences in the parotid salivary enzymes were strong with the powdered glucose 2000 diet and the pelleted forms of the glucose and sucrose 2000 and stock diets, and not significant with the powdered stock and sucrose 2000 diets.
  
  
  CONCLUSIONS
  Caries reportedly is higher in sucrose than glucose fed to rats in the standard powdered form of Diet 2000, mainly due to the colonizing advantage Streptococcus mutans gains with sucrose. These results suggest that additional factors are more feeding during lights on and less stimulation of parotid salivary secretion with the sucrose powder.
authors.affiliation: VA Medical Center, Dental Service, Oral Pathology Research Lab (151-1), 50 Irving Street, N.W., Washington, DC 20422, United States. Electronic address: bkredman@comcast.net.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Redman
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001835:Body Weight; D002940:Circadian Rhythm; D003731:Dental Caries / Q000139:chemically induced*; D004037:Diet, Cariogenic; D005247:Feeding Behavior; D005947:Glucose / Q000008:administration & dosage*; D010306:Parotid Gland / Q000201:enzymology*; D011208:Powders; D051381:Rats; D017207:Rats, Sprague-Dawley; D012463:Saliva / Q000201:enzymology*; D013395:Sucrose / Q000008:administration & dosage*
chemical_list: D011208:Powders; D013395:Sucrose; D005947:Glucose
keywords: Caries; Circadian rhythm; Diet; Parotid gland; Rat