title: Antioxidant activity of polysaccharides produced by Hirsutella sp. and relation with their chemical characteristics.
abstract: Extracellular polysaccharide (EPS) and intracellular polysaccharide (IPS) were produced in a mycelial liquid culture of the Hirsutella sp. liquid fermentation. The polysaccharides were precipitated with 50% ethanol (EPS-1, IPS-1), 65% ethanol (EPS-2, IPS-2) and 80% ethanol (EPS-3, IPS-3). The polysaccharide fragments precipitated in lower ethanol percentages had a lower neutral sugar content and a larger molecular weight. EPS-1, EPS-2, IPS-1 and IPS-2 were composed of glucose (Glu), galactose (Gal) and mannose (Man). Galactose was not detected in EPS-3 and IPS-3. Evaluated by the 1/IC50 values of hydroxyl radical scavenging activity, the polysaccharides with higher protein content, lower neutral sugar content and molecular weight about 10-20 kDa were found to have better radical scavenging activity. Significant correlations demonstrated that the antioxidant effect of the polysaccharides was influenced by monosaccharide composition (mannose, r=0.942; glucose, r=-0.905).
authors.affiliation: College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Meng
authors.affiliation: College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China.
authors.forename: Sasa
authors.initials: S
authors.lastname: Sun
authors.affiliation: Qingdao Women and Children Hospital, Qingdao, 266034, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China.
authors.forename: Zhaopeng
authors.initials: Z
authors.lastname: Shen
authors.affiliation: College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, PR China. Electronic address: jiangxl@ouc.edu.cn.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006430:Hemiptera / Q000378:metabolism*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000378:metabolism; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D017665:Hydroxyl Radical
keywords: Antioxidant activity; Correlation; Hirsutella sp.; Molecular weight; Monosaccharide composition; Polysaccharide