title: A short, rigid linker between pyrene and guanidiniocarbonyl-pyrrole induced a new set of spectroscopic responses to the ds-DNA secondary structure.
abstract: A novel pyrene-guanidiniocarbonyl-pyrrole dye, characterised by a short, rigid linker between the two chromophores, interacts strongly with ds-DNA but only negligibly with ds-RNA. Under neutral conditions the dye shows strong selectivity toward AT-DNA (with respect to GC-DNA). Binding is accompanied by a specific ICD band at 350 nm and fluorescence quenching for all DNAs/RNAs studied. At pH 5 the affinity of the dye is reversed, now favouring GC-DNA over AT-DNA. A strong emission increase for AT-DNA is observed but with quenching for GC-DNA.
authors.affiliation: Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, P. O. Box 180, HR-10002 Zagreb, Croatia. Ivo.Piantanida@irb.hr.
authors.forename: Marijana
authors.initials: M
authors.lastname: Radić Stojković
authors.forename: Patryciusz
authors.initials: P
authors.lastname: Piotrowski
authors.forename: Carsten
authors.initials: C
authors.lastname: Schmuck
authors.forename: Ivo
authors.initials: I
authors.lastname: Piantanida
mesh_terms: D004247:DNA / Q000737:chemistry*; D005470:Fluorometry; D006146:Guanidines / Q000737:chemistry*; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006146:Guanidines; D011721:Pyrenes; D011758:Pyrroles; D004247:DNA; C030984:pyrene