title: Novel co-enrichment method for isolation of magnetotactic bacteria.
abstract: A novel co-enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux. We successfully enriched and isolated both North seeking as well as South seeking magnetotactic bacteria from Lonar Lake (Buldhana), Moti Lake (Jalna), Ghanewadi Lake (Jalna), Ganesh Lake (Miraj), Rankala Lake (Kolhapur), and industrial metal-contaminated glaying soils (Jalna) and a soil (Karad), (MS, India) exposed to high-voltage electric current. The hanging drop preparations and growth under magnetic stress on low-agar media allowed conformation of magnetotactic behavior of the isolates. Both Gram positive and Gram negative MTB were isolated with diverse morphologies. South seeking population was more predominant. The soil inhabitants showed little dwelling property which was more prominent in case of aquatic inhabitants. The use of in situ pH and salt concentrations during enrichment and isolation found suited. The simultaneous growth of whole consortium in the system ensured the in situ simulation of microenvironment needful for proper growth of fastidious MTB.
authors.affiliation: PG Department of Microbiology, Yashwantrao Chavan College of Science, Karad (MS), India.
authors.forename: Ajay M
authors.initials: AM
authors.lastname: Sorty
authors.forename: Nasir R
authors.initials: NR
authors.lastname: Shaikh
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial; D019015:Geologic Sediments / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D007194:India; D060526:Magnetic Fields; D055944:Magnetosomes / Q000378:metabolism; D059013:Microbial Consortia; D010802:Phylogeny; D012988:Soil Microbiology; D013312:Stress, Physiological; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Isolation technique; Magnetotactic bacteria; Magnetotaxis; Sediment; Soil