title: Prevalence of multidrug resistant and extended spectrum beta-lactamase producing Pseudomonas aeruginosa in a tertiary care hospital.
abstract: Resistance to broad-spectrum beta-lactams, mediated by extended-spectrum beta-lactamase enzymes (ESBL), is an increasing problem worldwide. The present study was undertaken to determine the incidence of ESBL-production among the clinical isolates of Pseudomonas aeruginosa and their susceptibility to selected antimicrobials. A total of one eighty-seven clinical specimens were tested for the presence of ESBL production using the double-disc synergy test. Of these, 25.13% (n = 47) isolates of P. aeruginosa were observed as ESBL positive. The maximum number of ESBL-producing strains were found in sputum (41.67%; n = 24) followed by pus (28.36%; n = 19), cerebrospinal fluid and other body fluids (21.74%; n = 5), urine (20.45%; n = 9) and blood (13.79%; n = 4). ESBL producing isolates exhibited co-resistance to an array of antibiotics tested. Imipenem and meropenem can be suggested as the drugs of choice in our study.
authors.affiliation: Department of Biosciences, Integral University, Lucknow 226026, India.
authors.forename: Sibhghatulla
authors.initials: S
authors.lastname: Shaikh
authors.affiliation: Department of Bio-engineering, Integral University, Lucknow 226026, India.
authors.forename: Jamale
authors.initials: J
authors.lastname: Fatima
authors.affiliation: Department of Bio-engineering, Integral University, Lucknow 226026, India.
authors.forename: Shazi
authors.initials: S
authors.lastname: Shakil
authors.affiliation: Department of Biosciences, Integral University, Lucknow 226026, India.
authors.forename: Syed Mohd
authors.initials: SM
authors.lastname: Danish Rizvi
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia ; Enzymoic, 7 Peterlee Pl, Hebersham, NSW 2770, Australia.
authors.forename: Mohammad Amjad
authors.initials: MA
authors.lastname: Kamal
keywords: Double-disc synergy test; Extended-spectrum beta-lactamases; Imipenem; Meropenem; Pseudomonas aeruginosa