title: Effects and Mechanism of Baicalin on Apoptosis of Cervical Cancer HeLa Cells I n -v itro.
abstract: The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscope, and cell growth arrest was confirmed by flow cytometry. Moreover, Western blot was used for investigating the expression of apoptosis related proteins; spectrophotometry was used to examine Caspase-3 activation. Our results showed that baicalin could inhibit the proliferation of HeLa Cells via induction of apoptosis in a time and dose-dependent manner (P<0.01). Apoptotic signaling induced by baicalin was characterized by up-regulating Bax, Fas, FasL and Caspase-8 protein expression, and down-regulating of Bcl-2 protein expression. These results indicated that baicalin-induced apoptosis involved activation Caspase-3 in HeLa cells through the intracellular mitochondrial pathway and the surface death receptor pathway.
authors.affiliation: Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China. ; Department of Biological Engineering, College of Environment, Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: The First Hospital of Qinhuangdao Town, Qinhuangdao, 066100, China.
authors.forename: Zhan-Zhao
authors.initials: ZZ
authors.lastname: Fu
authors.affiliation: Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China.
authors.forename: Cong-Shan
authors.initials: CS
authors.lastname: Guo
authors.affiliation: Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: The First Hospital of Qinhuangdao Town, Qinhuangdao, 066100, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Di
authors.affiliation: Department of Biomedical Engineering, College of Electrical Engineering, Yanshan University, Qinhuangdao, 066004, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Department of Biological Engineering, College of Environment, Chemical Engineering, Yanshan University, Qinhuangdao, 066004, China. ; Department of Animal Science, Northwest Agriculture and Forestry University, Yangling, 712100, China.
authors.forename: Qing-Wang
authors.initials: QW
authors.lastname: Li
keywords: Baicalin; Caspase-3; Cell apoptosis; Death receptor pathway; HeLa cells; Mitochondrial pathway