title: Stress and strain analysis on the anastomosis site sutured with either epineurial or perineurial sutures after simulation of sciatic nerve injury.
abstract: The magnitude of tensile stress and tensile strain at an anastomosis site under physiological stress is an important factor for the success of anastomosis following suturing in peripheral nerve injury treatment. Sciatic nerves from fresh adult cadavers were used to create models of sciatic nerve injury. The denervated specimens underwent epineurial and perineurial suturing. The elastic modulus (40.96 ± 2.59 MPa) and Poisson ratio (0.37 ± 0.02) of the normal sciatic nerve were measured by strain electrical measurement. A resistance strain gauge was pasted on the front, back, left, and right of the edge of the anastomosis site after suturing. Strain electrical measurement results showed that the stress and strain values of the sciatic nerve following perineurial suturing were lower than those following epineurial suturing. Scanning electron microscopy revealed that the sciatic nerve fibers were disordered following epineurial compared with perineurial suturing. These results indicate that the effect of perineurial suturing in sciatic nerve injury repair is better than that of epineurial suturing.
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China.
authors.forename: Guangyao
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Ophthalmology, Second Hospital, Jilin University, Changchun 130041, Jilin Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Gao
keywords: Poisson ratio; anastomosis site; biomechanics; elastic modulus; epineurial suture; neural regeneration; perineurial suture; peripheral nerve injury; sciatic nerve injury; strain electrical measurement; tensile strain; tensile stress