title: Composite scaffolds of dicalcium phosphate anhydrate /multi-(amino acid) copolymer: in vitro degradability and osteoblast biocompatibility.
abstract: This study aims to evaluate in vitro degradability and osteoblast biocompatibility of dicalcium phosphate anhydrate/multi-(amino acid) (DCPA/MAA) composites prepared by in situ polymerization method. The results revealed that the composites could be slowly degraded in PBS solution, with weight loss of 9.5 ± 0.2 wt.% compared with 12.2 ± 0.2 wt.% of MAA copolymer after eight weeks, and the changes of pH value were in the range of 7.18-7.4 and stabilized at 7.24. In addition, the compressive strength of the composite decreased from 98 to 62 MPa while that of MAA copolymer from 117 to 86 MPa. Furthermore, with non-toxicity demonstrated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide assay, the addition of DCPA to the MAA copolymer evidenced an enhancement of osteoblast differentiation and attachment compared with pure MAA materials regarding to alkaline phosphatase activity as well as initial cell adhesion. The results indicated that the DCPA/MAA scaffolds with good osteoblast biocompatibility, degradability, and sufficient strength had promising potential application in bone tissue engineering.
authors.affiliation: a State Key Laboratory of Oral Diseases , Sichuan University , Chengdu 610041 , P.R. China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Ye
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.forename: Anchun
authors.initials: A
authors.lastname: Mo
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D010795:Phthalic Acids / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D017207:Rats, Sprague-Dawley; D012996:Solutions; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D010795:Phthalic Acids; D011108:Polymers; D012996:Solutions; C007220:dimethyl 2,3,5,6-tetrachloroterephthalate; C485829:calcium phosphate, dibasic, anhydrous
keywords: composite; degradation; dicalcium phosphate anhydrate; multi-(amino acid) copolymer; osteoblast biocompatibility