title: Revisiting the role of histo-blood group antigens in rotavirus host-cell invasion.
abstract: Histo-blood group antigens (HBGAs) have been proposed as rotavirus receptors. H type-1 and Lewis(b) antigens have been reported to bind VP8* from major human rotavirus genotypes P[4], P[6] and P[8], while VP8* from a rarer P[14] rotavirus recognizes A-type HBGAs. However, the role and significance of HBGA receptors in rotavirus pathogenesis remains uncertain. Here we report that P[14] rotavirus HAL1166 and the related P[9] human rotavirus K8 bind to A-type HBGAs, although neither virus engages the HBGA-specific α1,2-linked fucose moiety. Notably, human rotaviruses DS-1 (P[4]) and RV-3 (P[6]) also use A-type HBGAs for infection, with fucose involvement. However, human P[8] rotavirus Wa does not recognize A-type HBGAs. Furthermore, the common human rotaviruses that we have investigated do not use Lewis(b) and H type-1 antigens. Our results indicate that A-type HBGAs are receptors for human rotaviruses, although rotavirus strains vary in their ability to recognize these antigens.
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland 4222, Australia.
authors.forename: Raphael
authors.initials: R
authors.lastname: Böhm
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
authors.forename: Fiona E
authors.initials: FE
authors.lastname: Fleming
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland 4222, Australia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Maggioni
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
authors.forename: Vi T
authors.initials: VT
authors.lastname: Dang
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
authors.forename: Gavan
authors.initials: G
authors.lastname: Holloway
authors.affiliation: Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000, Australia.
authors.forename: Barbara S
authors.initials: BS
authors.lastname: Coulson
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland 4222, Australia.
authors.forename: Mark
authors.initials: M
authors.lastname: von Itzstein
authors.affiliation: Institute for Glycomics, Griffith University, Gold Coast Campus, Southport, Queensland 4222, Australia.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haselhorst
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D056004:Molecular Dynamics Simulation; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D012401:Rotavirus / Q000378:metabolism*; D012400:Rotavirus Infections / Q000378:metabolism / Q000503:physiopathology*; D053586:Virus Internalization
chemical_list: D000017:ABO Blood-Group System; D009844:Oligosaccharides