title: Comparative analysis of microbial community of novel lactic acid fermentation inoculated with different undefined mixed cultures.
abstract: Three undefined mixed cultures (activated sludge) from different municipal wastewater treatment plants were used as seeds in a novel lactic acid fermentation process fed with potato peel waste (PPW). Anaerobic sequencing batch fermenters were run under identical conditions to produce predominantly lactic acid. Illumina sequencing was used to examine the 16S rRNA genes of bacteria in the three seeds and fermenters. Results showed that the structure of microbial communities of three seeds were different. All three fermentation products had unique community structures that were dominated (>96%) by species of the genus Lactobacillus, while members of this genus constituted <0.1% in seeds. The species of Lactobacillus sp. differed among the three fermentations. Results of this study suggest the structure of microbial communities in lactic acid fermentation of PPW with undefined mixed cultures were robust and resilient, which provided engineering prospects for the microbial utilization of carbohydrate wastes to produce lactic acid.
authors.affiliation: Environmental Science Program, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Karol
authors.initials: K
authors.lastname: Gliniewicz
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Helena
authors.initials: H
authors.lastname: Mendes-Soares
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Settles
authors.affiliation: Department of Biological Sciences and the Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Larry J
authors.initials: LJ
authors.lastname: Forney
authors.affiliation: Department of Civil Engineering, University of Idaho, Moscow, ID 83844, United States.
authors.forename: Erik R
authors.initials: ER
authors.lastname: Coats
authors.affiliation: Environmental Science Program, University of Idaho, Moscow, ID 83844, United States; Renewable Materials Program, Department of Forest, Rangeland and Fire Sciences, University of Idaho, Moscow, ID 83844, United States. Electronic address: armandm@uidaho.edu.
authors.forename: Armando G
authors.initials: AG
authors.lastname: McDonald
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D044822:Biodiversity; D018533:Biomass; D002947:Cities; D005285:Fermentation; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D062065:Wastewater / Q000382:microbiology; D018508:Water Purification
chemical_list: D019344:Lactic Acid; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Illumina sequencing; Lactic acid; Lactobacillus; Microbial community; Potato peel waste