title: RVLM-IML pathway may implicate controlling peripheral airways by melanocortinergic-sympathetic signaling: a transneuronal labeling study using pseudorabies virus.
abstract: Pseudorabies virus (PRV)-614 was injected into the tracheal wall of male MC4R-GFP transgenic mice, resulting in retrograde infections in spinal cord and autonomic premotor areas of the brain including the rostroventrolateral medulla (RVLM). This polysynaptic pathway to the airway may form the substrate underlying the impact of IML and RVLM on airway function. The neurochemical phenotype of MC4R-GFP-positive neurons was identified using fluorescence immunocytochemical labeling. PRV-614/MC4R-GFP dual labeled neurons were detected in spinal IML and the rostral ventrolateral medulla (RVLM). These data demonstrate the RVLM-IML pathway of synaptically connected neurons extending to the airway through melanocortinergic-sympathetic signaling.
authors.affiliation: Department of Anesthesiology, Maternal and Child Health Hospital of Hubei Province Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Cheng-Jin
authors.initials: CJ
authors.lastname: Yue
authors.affiliation: Department of Anesthesiology and Pain Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
authors.affiliation: Department of Thoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan 430030, Hubei, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
mesh_terms: D000818:Animals; D011558:Herpesvirus 1, Suid; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D051379:Mice; D009434:Neural Pathways / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D044105:Receptor, Melanocortin, Type 4 / Q000378:metabolism*; D012119:Respiration; D015398:Signal Transduction / Q000502:physiology*; D013116:Spinal Cord / Q000378:metabolism*; D013564:Sympathetic Nervous System / Q000378:metabolism*
chemical_list: C574273:MC4R protein, mouse; D044105:Receptor, Melanocortin, Type 4
keywords: Peripheral airway; autonomic nervous system; melanocortin-4 receptor; pseudorabies virus; spinal cord; the rostroventrolateral medulla; transsynaptic tracing