title: The role of hepcidin and its related genes (BMP6, GDF-15, and HJV) in rats exposed to ischemia and reperfusion.
abstract: OBJECTIVE
  To determine the roles of hepcidin and its related genes in a renal ischemia/reperfusion model.
  
  
  METHODS
  A total of 20 Wistar albino rats were equally divided into 2 groups: Group I was the control group and Group II was the ischemia and reperfusion (I/R) group (60 min of ischemia + 48 h of reperfusion). I/R was performed on the left kidneys of these rats and then the I/R-treated kidneys were removed. The levels of serum biochemical markers were evaluated after renal I/R. The expression levels of hepcidin-linked genes [growth differentiation factor 15 (GDF-15), bone morphogenetic protein 6 (BMP6), and hemojuvelin (HJV)] were also measured by RT-PCR technique. In addition, the tissues were evaluated histopathologically.
  
  
  RESULTS
  No significant association was found between renal dysfunction and I/R when compared to biochemical parameters (P > 0.05). However, differences in platelet values were statistically significant (P < 0.05). Expression levels of GDF-15, BMP6, and HJV genes increased, but this increase was not statistically significant. In addition, histopathological evaluation was performed using hematoxylin and eosin stain. This showed a significant relationship between the control group and I/R group for ischemic and nonischemic kidney scoring.
  
  
  CONCLUSIONS
  Hepcidin and BMP6, HJV, and GDF-15 should be taken into account when investigating the process of I/R.
authors.affiliation: Department of Medical Physiology, Mustafa Kemal University, Hatay, Turkey. drecepfatih@gmail.com
authors.forename: Recep
authors.initials: R
authors.lastname: Dokuyucu
authors.forename: Tuncer
authors.initials: T
authors.lastname: Demir
authors.lastname: Yumrutaş Onder
authors.forename: Ayşe Binnur
authors.initials: AB
authors.lastname: Erbağci
authors.forename: Mustafa
authors.initials: M
authors.lastname: Orkmez
authors.forename: Abdulkadir Yasir
authors.initials: AY
authors.lastname: Bahar
authors.forename: Recep
authors.initials: R
authors.lastname: Bayraktar
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Bozgeyik
authors.forename: Davut Sinan
authors.initials: DS
authors.lastname: Kaplan
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Beyhan Cengiz
authors.initials: BC
authors.lastname: Bağci
mesh_terms: D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000235:genetics* / Q000378:metabolism; D004195:Disease Models, Animal; D058851:GPI-Linked Proteins; D055436:Growth Differentiation Factor 15 / Q000235:genetics* / Q000378:metabolism; D000071020:Hemochromatosis Protein; D064451:Hepcidins / Q000097:blood*; D007668:Kidney / Q000293:injuries / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C525818:Bmp6 protein, rat; D055418:Bone Morphogenetic Protein 6; D058851:GPI-Linked Proteins; C526142:Gdf15 protein, rat; D055436:Growth Differentiation Factor 15; D000071020:Hemochromatosis Protein; D064451:Hepcidins; C513726:Hjv protein, rat; D008565:Membrane Proteins