title: Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803.
abstract: Here, we report the photosynthetic production of itaconic acid (IA), a promising building block, from carbon dioxide (CO₂) by Synechocystis sp. PCC6803. The engineered PCC6803 strain expressing cis-aconitate decarboxylase, the key enzyme in IA biosynthesis, produced 0.9 mg/L and 14.5 mg/L of IA at production rates of 42.8 μgL(-1)day(-1) and 919.0 μgL(-1)day(-1), under conditions of constant bubbling with air and 5% CO₂, respectively. This is the first report on the possibility of IA production from CO₂ via the photosynthetic process in cyanobacteria.
authors.affiliation: Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Taejun
authors.initials: T
authors.lastname: Chin
authors.affiliation: Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Mei
authors.initials: M
authors.lastname: Sano
authors.affiliation: Faculty of Education, Shimane University, 1060 Nishikawatsu-cho, Matsue, Shimane 690-8504, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Ohara
authors.affiliation: Department of Biobased Materials Science, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address: aso@kit.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Aso
mesh_terms: D002262:Carboxy-Lyases / Q000378:metabolism; D049109:Cell Proliferation; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways; D010788:Photosynthesis / Q000502:physiology*; D013386:Succinates / Q000032:analysis / Q000378:metabolism*; D046939:Synechocystis / Q000235:genetics / Q000378:metabolism*
chemical_list: D013386:Succinates; D002262:Carboxy-Lyases; C018742:aconitate decarboxylase; C005229:itaconic acid
keywords: Itaconic acid; Photosynthesis; Synechocystis sp. PCC6803; cis-Aconitate decarboxylase