title: Anti-metastatic effect and mechanisms of Wenshen Zhuanggu Formula in human breast cancer cells.
abstract: BACKGROUND
  Wenshen Zhuanggu Formula (WSZG), a traditional Chinese medicine (TCM) empirical prescription, has been used to treat the patients with breast cancer bone metastasis as an adjuvant in clinical practice. To explore the anti-metastatic activity and potential mechanisms of WSZG-containing serum (WSZG-CS) on highly bone-metastatic human breast cancer MDA-MB-231BO cells.
  
  
  METHODS
  MDA-MB-231BO cells were cultured alone or co-cultured with bone marrow-derived mesenchymal stem cells (BMSCs). Invasion assays were carried out in Matrigel-coated Transwell chambers. CC chemokine 5 (CCL5) and interleukin (IL)-17B secretion levels were detected by ELISA. CCR5 and IL-17BR protein expression levels were determined by immunocytochemistry and Western blot analysis.
  
  
  RESULTS
  Compared with control serum, WSZG-CS significantly inhibited BMSC induced MDA-MB-231BO breast cancer cell invasion, reduced CCL5 and IL-17B levels in co-culture supernatants, and downregulated CCR5 and IL-17BR protein expression in breast cancer cells co-cultured with BMSCs.
  
  
  CONCLUSIONS
  WSZG-CS exerts an anti-metastatic activity against MDA-MB-231BO breast cancer cells, due to its ability to mitigate the interaction between BMSCs and breast cancer cells mediated via the CCL5/CCR5 and IL-17B/IL-17BR signaling pathways.
authors.affiliation: Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 725 Wanping South Road, Shanghai 200032, PR China.
authors.forename: Xiang-hui
authors.initials: XH
authors.lastname: Han
authors.affiliation: Engineering Research Center of Modernization of Traditional Chinese Medicine, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Chun-li
authors.initials: CL
authors.lastname: Wang
authors.affiliation: Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, PR China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Ma
authors.affiliation: Engineering Research Center of Modernization of Traditional Chinese Medicine, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 725 Wanping South Road, Shanghai 200032, PR China.
authors.forename: Qian-wen
authors.initials: QW
authors.lastname: Hu
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, PR China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.affiliation: Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 725 Wanping South Road, Shanghai 200032, PR China. Electronic address: yigels2014@126.com.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Institute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 725 Wanping South Road, Shanghai 200032, PR China. Electronic address: lujingen_lh@126.com.
authors.forename: Jin-gen
authors.initials: JG
authors.lastname: Lu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000378:metabolism; D018920:Coculture Techniques; D003374:Coumarins / Q000032:analysis; D005260:Female; D005363:Ficusin / Q000032:analysis; D011564:Furocoumarins / Q000032:analysis; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D059630:Mesenchymal Stem Cells / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D044967:Serum
chemical_list: D000970:Antineoplastic Agents; C483024:CCL5 protein, human; D018946:Chemokine CCL5; D003374:Coumarins; D011564:Furocoumarins; D020381:Interleukin-17; D010936:Plant Extracts; C000605708:Wenshen Zhuanggu; C011659:angelicin; D005363:Ficusin; C046627:osthol
keywords: Breast cancer; Isopsoralen (PubChem CID: 10658); Mesenchymal stem cells; Metastases; Osthole (PubChem CID: 10228); Psoralen (PubChem CID: 6199); Traditional Chinese medicine; Wenshen Zhuanggu Formula