title: Production and characterization of highly specific monoclonal antibodies to D-glutamic acid.
abstract: Most of the functions of D-amino acids (D-AA) remain unclear because of little analytic methods for specific detection/determination. In this study, a highly specific monoclonal antibody to D-glutamic acid (D-Glu-MAb) was produced using a hybridoma method. Characterization of D-Glu-MAb by indirect enzyme-linked immunosorbent assay (ELISA) revealed that it has high selectivity against D-Glu-glutaraldehyde (GA) conjugates, while no cross-reaction was observed when 38 other kinds of AA-GA conjugates were used. Moreover, subsequent indirect competitive ELISA disclosed that an epitope of D-Glu-MAb is a D-Glu-GA molecule in the conjugates, suggesting that D-Glu-MAb could be a useful tool to investigate the functional analysis of D-Glu in immunostaining.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyushu University , Higashi-ku, Fukuoka, Japan .
authors.forename: Seiichi
authors.initials: S
authors.lastname: Sakamoto
authors.forename: Yurino
authors.initials: Y
authors.lastname: Matsuura
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Yonenaga
authors.forename: Yumi
authors.initials: Y
authors.lastname: Tsuneura
authors.forename: Mariko
authors.initials: M
authors.lastname: Aso
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Kurose
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D018698:Glutamic Acid / Q000737:chemistry / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure
chemical_list: D000911:Antibodies, Monoclonal; D018698:Glutamic Acid