title: A Candidate Single Nucleotide Polymorphism in the 3' Untranslated Region of Stearoyl-CoA Desaturase Gene for Fatness Quality and the Gene Expression in Berkshire Pigs.
abstract: Fatness qualities in pigs measured by the amount of fat deposition and composition of fatty acids (FAs) in pork have considerable effect on current breeding goals. The stearoyl-CoA desaturase (SCD) gene plays a crucial role in the conversion of saturated FAs into monounsaturated FAs (MUFAs), and hence, is among the candidate genes responsible for pig fatness traits. Here, we identified a single nucleotide polymorphism (SNP, c.*2041T>C) in the 3' untranslated region by direct sequencing focused on coding and regulatory regions of porcine SCD. According to the association analysis using a hundred of Berkshire pigs, the SNP was significantly associated with FA composition (MUFAs and polyunsaturated FAs [PUFAs]), polyunsaturated to saturated (P:S) FA ratio, n-6:n-3 FA ratio, and extent of fat deposition such as intramuscular fat and marbling (p<0.05). In addition, the SNP showed a significant effect on the SCD mRNA expression levels (p = 0.041). Based on our results, we suggest that the SCD c.*2041T>C SNP plays a role in the gene regulation and affects the fatness qualities in Berkshire pigs.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
authors.forename: Kyu-Sang
authors.initials: KS
authors.lastname: Lim
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea ; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, 4072, Australia .
authors.forename: Jun-Mo
authors.initials: JM
authors.lastname: Kim
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
authors.forename: Eun-A
authors.initials: EA
authors.lastname: Lee
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
authors.forename: Jee-Hwan
authors.initials: JH
authors.lastname: Choe
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
authors.forename: Ki-Chang
authors.initials: KC
authors.lastname: Hong
keywords: Berkshire; Fatness Quality; Gene Expression; Polymorphism; Stearoyl-CoA Desaturase [SCD]