title: Applications of flow cytometry for measurement of autophagy.
abstract: Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy.
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Alik
authors.initials: A
authors.lastname: Demishtein
authors.affiliation: Department of Biological Services, The Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: Ziv
authors.initials: Z
authors.lastname: Porat
authors.affiliation: Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel. Electronic address: zvulun.elazar@weizmann.ac.il.
authors.forename: Zvulun
authors.initials: Z
authors.lastname: Elazar
authors.affiliation: Department of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK. Electronic address: eshvets@mrc-lmb.cam.ac.uk.
authors.forename: Elena
authors.initials: E
authors.lastname: Shvets
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis*; D000818:Animals; D001343:Autophagy; D005434:Flow Cytometry / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D000071456:Sequestosome-1 Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D006360:Heat-Shock Proteins; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D000071456:Sequestosome-1 Protein; C101343:Sqstm1 protein, mouse; D049452:Green Fluorescent Proteins
keywords: Autophagy; Flow cytometry; GFP–LC3; Lysosome; P62/SQSTM1