title: Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates.
abstract: The aerobic Pseudomonas denitrificans is widely used for industrial and commercial vitamin B12 fermentation, due to its higher productivity compared to the anaerobic vitamin B12-producing microorganisms. This paper aimed to develop a cost-effective fermentation medium for industrial vitamin B12 production by P. denitrificans in 120,000-l fermenter. It was found that maltose syrup (a low-cost syrup from corn starch by means of enzymatic or acid hydrolysis) and corn steep liquor (CSL, a by-product of starch industry) were greatly applicable to vitamin B12 production by P. denitrificans. Under the optimal fermentation medium performed by response surface methodology, 198.27 ± 4.60 mg/l of vitamin B12 yield was obtained in 120,000-l fermenter, which was close to the fermentation with the refined sucrose (198.80 mg/l) and was obviously higher than that obtained under beet molasses utilization (181.75 mg/l). Therefore, maltose syrups and CSL were the efficient and economical substrates for industrial vitamin B12 fermentation by P. denitrificans.
authors.affiliation: Nanchang Key Laboratory of Applied Fermentation Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Wei-Fu
authors.initials: WF
authors.lastname: Peng
authors.forename: Kun-Tai
authors.initials: KT
authors.lastname: Li
mesh_terms: D019149:Bioreactors; D003362:Cost-Benefit Analysis; D005285:Fermentation; D008320:Maltose / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D014805:Vitamin B 12 / Q000096:biosynthesis*; D003313:Zea mays / Q000378:metabolism*
chemical_list: D008320:Maltose; D014805:Vitamin B 12