title: Improved surgical margin definition by narrow band imaging for resection of oral squamous cell carcinoma: A prospective gene expression profiling study.
abstract: Incomplete primary tumor excision contributes to localized postsurgical recurrence of oral squamous cell carcinoma (OSCC). The purpose of this study was to provide molecular evidence that surgical margin definition using narrow band imaging (NBI) resulted in more complete OSCC excision than conventional white light (WL) panendoscopy.
  
  
  
  Molecular divergence among tumor, WL, and NBI-defined surgical margins was compared in 18 patients through microarray analysis (GeneChip U133-plus-2.0).
  
  
  
  The numbers of differentially expressed genes (NBI = 4387; WL = 3266; vs tumor) signified that NBI placed margins into less involved tissue than WL examination. Principal component analysis segregated tumor, WL, and NBI tissues appropriately based solely on mRNA profiles, and unsupervised hierarchical clustering identified 4 patients (22%) who benefited directly from NBI surgical margin definition. Gene ontology enrichment indicated increasing cell phenotypic diversity: tumor<WL<NBI.
  
  
  
  Resection to NBI-defined margins will leave less dysplastic and malignant residual tissue and thereby increase ablative surgery success rates. © 2015 Wiley Periodicals, Inc. Head Neck 38: 832-839, 2016.
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia.|Australian Centre for Oral Oncology Research and Education, Brisbane, Queensland, Australia.
authors.forename: Camile S
authors.initials: CS
authors.lastname: Farah
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Andrew J
authors.identifier: 0000-0002-8362-6492
authors.initials: AJ
authors.lastname: Dalley
authors.affiliation: Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, Australia.
authors.forename: Phan
authors.initials: P
authors.lastname: Nguyen
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia.|Maxillofacial Unit, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia.
authors.forename: Martin
authors.initials: M
authors.lastname: Batstone
authors.affiliation: University of Queensland Centre for Clinical Research, The University of Queensland, Brisbane, Queensland, Australia.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Kordbacheh
authors.affiliation: Department of Pathology, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia.
authors.forename: Joanna
authors.initials: J
authors.lastname: Perry-Keene
authors.affiliation: Department of Thoracic Medicine, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Fielding
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D000072662:Margins of Excision; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging* / Q000235:genetics / Q000473:pathology / Q000601:surgery; D062048:Narrow Band Imaging; D011446:Prospective Studies; D012334:RNA, Neoplasm / Q000378:metabolism
chemical_list: D012334:RNA, Neoplasm
keywords: microarray analysis; narrow band imaging; oral squamous cell carcinoma; prevention of second cancers; surgical margins