title: [HPLC analysis of dehydroepiandrosterone sulfate in serum with use of solid-phase extraction on hyper cross-linked polystyrene (Purosep-200)].
abstract: We have developed a simple HPLC method for analysis of the dehydroepiandrosterone sulfate (DHEA-sulfate) in serum with use a new procedure of solid-phase extraction (SPE) on hyper cross-linked polystyrene (Purosep-200) and fast chromatographic separation on the monolithic column under isocratic elution and UV detection at 200 nm. Complete SPE procedure lasts for about 7 min, chromatographic separation takes less than 6 min. Simplicity and high reproducibility of this method makes it attractive in routine clinical practice.
authors.affiliation: State Medical Academy.
authors.forename: A A
authors.initials: AA
authors.lastname: Dutov
authors.affiliation: Zabaikalsky State University, Chita, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Nikitin
authors.affiliation: State Medical Academy.
authors.forename: Yu L
authors.initials: YL
authors.lastname: Luk'ianova
authors.affiliation: Zabaikalsky State University, Chita, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Sverkunova
authors.affiliation: Zabaikalsky State University, Chita, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Martynova
authors.affiliation: State Medical Academy.
authors.forename: A V
authors.initials: AV
authors.lastname: Ermolina
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000592:standards*; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D011137:Polystyrenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation*
chemical_list: D011137:Polystyrenes; D019314:Dehydroepiandrosterone Sulfate
keywords: HPLC; dehydroepiandrosterone sulfate; hyper cross-linked polystyrene (Purosep-200); serum; solid-phase extraction