title: Differential expression of perilipin 2 and 5 in human skeletal muscle during aging and their association with atrophy-related genes.
abstract: Sarcopenia, the progressive loss of muscle mass and strength, is a phenomenon characterizing human aging whose etiology is still not clear. While there is increasing evidence for the influence of inter-muscular adipose tissue infiltration in the development of sarcopenia, much less is known about a possible role for intra-muscular triglycerides (IMTG). IMTG accumulate in form of lipid droplets decorated by proteins such as Perilipins (Plins). In skeletal muscle the most abundant are Plin2 and Plin5. In this study we compared the expression of these two Plins in Vastus lateralis muscle samples of subjects of different age, both healthy donors (HD) and patients with limited lower limb mobility (LLMI). These latter are characterized by a condition of chronic physical inactivity. Plin2 expression resulted higher in old age for both HD and LLMI patients, while Plin5 slightly decreased only in LLMI patients. Moreover, in these patients, only Plin2 was associated with the decrease of muscle strength and the expression of factors related to muscle atrophy (MuRF1, Atrogin and p53). An increase in Plin2 and a concomitant decrease of Plin5 was also observed when we considered animal model of disuse-induced muscle atrophy. As a whole, these data indicate that Plin2 and Plin5 have a different expression pattern during muscle aging and inactivity, and only Plin2 appears to be associated with functional alterations of the muscle.
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine and Interdepartmental Centre "L. Galvani" (CIG), University of Bologna, via S. Giacomo 12, 40126, Bologna, Italy, m.conte@unibo.it.
authors.forename: Maria
authors.initials: M
authors.lastname: Conte
authors.forename: Francesco
authors.initials: F
authors.lastname: Vasuri
authors.forename: Enrico
authors.initials: E
authors.lastname: Bertaggia
authors.forename: Andrea
authors.initials: A
authors.lastname: Armani
authors.forename: Aurelia
authors.initials: A
authors.lastname: Santoro
authors.forename: Elena
authors.initials: E
authors.lastname: Bellavista
authors.forename: Alessio
authors.initials: A
authors.lastname: Degiovanni
authors.forename: Antonia
authors.initials: A
authors.lastname: D'Errico-Grigioni
authors.forename: Giovanni
authors.initials: G
authors.lastname: Trisolino
authors.forename: Miriam
authors.initials: M
authors.lastname: Capri
authors.forename: Martino V
authors.initials: MV
authors.lastname: Franchi
authors.forename: Marco V
authors.initials: MV
authors.lastname: Narici
authors.forename: Marco
authors.initials: M
authors.lastname: Sandri
authors.forename: Claudio
authors.initials: C
authors.lastname: Franceschi
authors.forename: Stefano
authors.initials: S
authors.lastname: Salvioli
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001706:Biopsy; D016022:Case-Control Studies; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008875:Middle Aged; D051346:Mobility Limitation; D023421:Models, Animal; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D053580:Muscle Strength / Q000502:physiology; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D009133:Muscular Atrophy / Q000235:genetics / Q000378:metabolism*; D000070780:Perilipin-2; D000070797:Perilipin-5; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D055948:Sarcopenia / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D009124:Muscle Proteins; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; D000070797:Perilipin-5; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; C000602983:Plin2 protein, mouse; C511805:Plin5 protein, mouse; C485290:Ppargc1a protein, mouse; D011506:Proteins; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D016159:Tumor Suppressor Protein p53; C440908:TRIM63 protein, human; D044767:Ubiquitin-Protein Ligases