title: From the viral perspective: infectious salmon anemia virus (ISAV) transcriptome during the infective process in Atlantic salmon (Salmo salar).
abstract: The infectious salmon anemia virus (ISAV) is a severe disease that mainly affects the Atlantic salmon (Salmo salar) aquaculture industry. Although several transcriptional studies have aimed to understand Salmon-ISAV interaction through the evaluation of host-gene transcription, none of them has focused their attention upon the viral transcriptional dynamics. For this purpose, RNA-Seq and RT-qPCR analyses were conducted in gills, liver and head-kidney of S. salar challenged by cohabitation with ISAV. Results evidence the time and tissue transcript patterns involved in the viral expression and how the transcription levels of ISAV segments are directly linked with the protein abundance found in other virus of the Orthomyxoviridae family. In addition, RT-qPCR result evidenced that quantification of ISAV through amplification of segment 3 would result in a more sensitive approach for detection and quantification of ISAV. This study offers a more comprehensive approach regarding the ISAV infective process and gives novel knowledge for its molecular detection.
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Concepción, Chile; Aquainnovo, Casilla 30B, Puerto Montt 5503032, Chile.
authors.forename: Diego
authors.initials: D
authors.lastname: Valenzuela-Miranda
authors.affiliation: Facultad de Ciencias Agronómicas, Universidad de Chile, Av Santa Rosa 11315, La Pintana, Santiago 8820808, Chile.
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Cabrejos
authors.affiliation: Aquainnovo, Casilla 30B, Puerto Montt 5503032, Chile; Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Av Santa Rosa 11735, La Pintana, Santiago 8820808, Chile.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Yañez
authors.affiliation: Laboratory of Biotechnology and Aquatic Genomics, Interdisciplinary Center for Aquaculture Research (INCAR), University of Concepción, P.O. Box 160-C, Concepción, Chile. Electronic address: crisgallardo@udec.cl.
authors.forename: Cristian
authors.initials: C
authors.lastname: Gallardo-Escárate
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D046829:Isavirus / Q000235:genetics*; D020317:Salmo salar; D059467:Transcriptome
keywords: ISAV; Orthomyxoviridae; RNA-Seq; Salmo salar; Viral transcriptome