title: Determinants of plasma renin activity: role of a human renin gene variant as a genetic factor.
abstract: The plasma renin activity (PRA) is affected by a number of environmental factors. However, significant heritability has been shown for the activity. A hypothesis that a candidate regulatory single-nucleotide polymorphism, C-5312T, of human renin gene should have a significant effect on PRA was elucidated and updating of independent determinants of PRA was attempted. Cross sectional study. Outpatient study. We enrolled consecutive 810 subjects who had consulted our hospitals for lifestyle-related diseases. Genotypes were assayed with genomic DNA for C-5312T. Among the genetic variants, the difference of PRA was evaluated. Monovariate linear regression analysis was performed to test the correlation between PRA and clinical variables. Finally, stepwise multiple regression analysis was performed to evaluate the independent determinants. On comparing 2 genotype groups, CC/CT and T allele homozygote, the geometric means of PRA were 0.778 and 0.941 ng/ml/h, respectively (F = 5.992, P = 0.015). Monovariate linear regression analysis revealed that a number of variables have a significant correlation with the activity, including urinary salt excretion. A stepwise multivariate regression analysis revealed that renin C-5312T variant (TT) is one of the independent determinants of PRA. Thus, for the first time, a human renin gene variant was associated with a significant increase in PRA as a genetic factor and the independent determinants for the activity were updated including genetic factor.
authors.affiliation: From the Third Department of Internal Medicine, University of Fukui Faculty of Medical Sciences, Fukui (TK, TN, AS, MY, MI, SS, MI, MF, KY, YM, KA, JS, TI); and Department of Environmental and Preventive Medicine, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan (HN).
authors.forename: Tadashi
authors.initials: T
authors.lastname: Konoshita
authors.forename: Takahiro
authors.initials: T
authors.lastname: Nakaya
authors.forename: Ai
authors.initials: A
authors.lastname: Sakai
authors.forename: Mika
authors.initials: M
authors.lastname: Yamada
authors.forename: Mai
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Satsuki
authors.initials: S
authors.lastname: Sato
authors.forename: Michiko
authors.initials: M
authors.lastname: Imagawa
authors.forename: Miki
authors.initials: M
authors.lastname: Fujii
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Makino
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Arakawa
authors.forename: Jinya
authors.initials: J
authors.lastname: Suzuki
authors.forename: Tamotsu
authors.initials: T
authors.lastname: Ishizuka
mesh_terms: D000367:Age Factors; D000483:Alleles; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D001835:Body Weight; D003430:Cross-Sectional Studies; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D012083:Renin / Q000097:blood* / Q000235:genetics*; D012737:Sex Factors; D012964:Sodium / Q000652:urine; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D014280:Triglycerides; D012964:Sodium; D012083:Renin