title: Structural and genetic relationships of closely related O-antigens of Cronobacter spp. and Escherichia coli: C. sakazakii G2594 (serotype O4)/E. coli O103 and C. malonaticus G3864 (serotype O1)/E. coli O29.
abstract: O-Antigen (O-polysaccharide) variation is the basis for bacterial serotyping and is important in bacterial virulence and niche adaptation. In this work, we present structural and genetic evidences for close relationships between the O-antigens of the Cronobacter spp. and Escherichia coli. Cronobacter sakazakii G2594 (serotype O4) and Cronobacter malonaticus G3864 (serotype O1) are structurally related to those of E. coli O103 and O29, respectively, and some other members of the Enterobacteriaceae family differing in the patterns of lateral glucosylation (C. sakazakii G2594) or O-acetylation (C. malonaticus G3864). The O-antigen gene clusters of the corresponding Cronobacter and E. coli strains contain the same genes with high-level similarity, and the structural differences within both O-antigen pairs were suggested to be due to modification genes carried by prophages.
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Eldar M
authors.initials: EM
authors.lastname: Turdymuratov
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Hu
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Guo
authors.affiliation: TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; Tianjin Biochip Corporation, TEDA, Tianjin 300457, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: yknirel@gmail.com.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D002240:Carbohydrate Sequence; D059126:Cronobacter / Q000145:classification* / Q000378:metabolism; D004926:Escherichia coli / Q000145:classification* / Q000378:metabolism; D019143:Evolution, Molecular; D005810:Multigene Family; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D065288:Serogroup
chemical_list: D019081:O Antigens
keywords: Bacterial polysaccharide structure; Cronobacter malonaticus; Cronobacter sakazakii; Lipopolysaccharide; O-Antigen gene cluster; O-Specific polysaccharide