title: MicroRNA-451 plays a role in murine embryo implantation through targeting Ankrd46, as implicated by a microarray-based analysis.
abstract: OBJECTIVE
  To determine the potential microRNA (miRNA) regulators of embryo implantation, as a continuation of genomic and proteomic research.
  
  
  METHODS
  Laboratory animal research.
  
  
  METHODS
  University hospital laboratory.
  
  
  METHODS
  Adult healthy female C57BL6/J mice (age 6-8 weeks, nonfertile, weighing 18-20 g each).
  
  
  METHODS
  Female mice were mated naturally with fertile males to produce pregnancy. Luminal epithelium was collected by laser-capture microdissection during the implantation period. Mouse models of pseudopregnancy, delayed implantation, and artificial decidualization were established.
  
  
  METHODS
  The miRNA profile in luminal epithelium was clarified by microarray analysis and validated by real-time reverse transcription polymerase chain reaction (qRT-PCR) in a series of models. Target genes were predicted and confirmed by luciferase activity assay. The role of miRNA in implantation was examined by loss-of-function and gain-of-function of miRNA in vitro and in vivo.
  
  
  RESULTS
  A total of 29 and 15 miRNAs were up- and down-regulated, respectively, during the implantation period; 11 of these miRNAs were validated by qRT-PCR. The profile of miR-451 was clarified in a series of models. A dual-luciferase activity assay showed that Ankrd46 was a target gene of miR-451. Loss-of-function by LV-miR-451 sponge or miR-451 inhibitor led to a reduced number of embryo implantations, but had little effect on fertilization.
  
  
  CONCLUSIONS
  miR-451 was specifically up-regulated during the implantation period, and it may play a major role in embryo implantation by targeting Ankrd46.
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China. Electronic address: zhengyuli@scu.edu.cn.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Jinhai
authors.initials: J
authors.lastname: Gou
authors.affiliation: Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China; Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Sichuan Key Laboratory of Gynecologic Oncology, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
mesh_terms: D000818:Animals; D010064:Embryo Implantation / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D046228:Microarray Analysis; D009124:Muscle Proteins / Q000235:genetics*; D041681:NIH 3T3 Cells; D011247:Pregnancy
chemical_list: C000597438:Ankrd46 protein, mouse; D035683:MicroRNAs; C550758:Mirn451 microRNA, mouse; D009124:Muscle Proteins
keywords: Ankrd46; Embryo implantation; luminal epithelium; miR-451; miRNA