title: Identification and validation of a new male sex-specific ISSR marker in pointed gourd (Trichosanthes dioica Roxb.).
abstract: The aim of the present study was to develop a genetic sex marker for the pointed gourd (Trichosanthes dioica Roxb.) to allow gender determination at any stage in the life cycle. Screening of genomic DNA with intersimple sequence repeat (ISSR) primers was used to discover sex-specific touch-down polymerase chain reaction (Td-PCR) amplification products. Using pooled DNA from male and female genotypes and 42 ISSR primers, a putative male specific marker (~550 bp) was identified. DNA marker specific to male is an indication of existence of nonepigenetic factors involved in gender development in pointed gourd. The ISSR technique has proved to be a reliable technique in gender determination of pointed gourd genotypes at the seedling phenophase. The sex marker developed here could also be used as a starting material towards sequence characterization of sex linked genes for better understanding the developmental as well as evolutionary pathways in sexual dimorphism.
authors.affiliation: Cytogenetics & Plant Breeding Section, Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
authors.forename: Sinchan
authors.initials: S
authors.lastname: Adhikari
authors.affiliation: Cytogenetics & Plant Breeding Section, Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
authors.forename: Soumen
authors.initials: S
authors.lastname: Saha
authors.affiliation: Department of Molecular Biology & Biotechnology, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
authors.forename: Tapas Kumar
authors.initials: TK
authors.lastname: Bandyopadhyay
authors.affiliation: Cytogenetics & Plant Breeding Section, Plant Biotechnology Research Unit, Department of Botany, University of Kalyani, Kalyani, Nadia, West Bengal 741235, India.
authors.forename: Parthadeb
authors.identifier: 0000-0002-0463-8429
authors.initials: P
authors.lastname: Ghosh
mesh_terms: D018744:DNA, Plant / Q000235:genetics*; D018745:Genome, Plant; D005838:Genotype; D019849:Sex Determination Processes; D029778:Trichosanthes / Q000235:genetics*
chemical_list: D018744:DNA, Plant