title: Expression profile of Toll-like receptor mRNA in pigs co-infected with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
abstract: Field and experimental studies have shown that co-infection of pigs with porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) increases the severity of the disease. The present study investigates the mRNA expression profile of Toll-like receptors (TLRs) in pigs co-infected with PRRSV and PCV2. SPF pigs were infected with PRRSV, PCV2 or in a combination of both. The mRNA expression levels of TLRs and related cytokines in peripheral blood mononuclear cells (PBMCs) of pigs were determined by quantitative real-time RT-PCR. The mRNA expression profiles of TLRs by PBMCs from pigs co-infected with PRRSV and PCV2 displayed two distinct patterns: an increased expression profile for TLRs2, 4 and 8, and a decreased expression profile for TLRs3, 7 and 9. An up-regulated expression of IL-1β and IL-10 mRNA and a down-regulated expression of INF-α and TNF-α mRNA in PBMCs of co-infected pigs were also observed.
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
authors.forename: Pang-Yan
authors.initials: PY
authors.lastname: Tu
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
authors.forename: Pei-Chun
authors.initials: PC
authors.lastname: Tsai
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
authors.forename: Yi-Hsin
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
authors.forename: Po-Cheng
authors.initials: PC
authors.lastname: Liu
authors.affiliation: Department of Animal Science, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan. Electronic address: hlachang@mail.npust.edu.tw.
authors.forename: Hsiu-luan
authors.initials: HL
authors.lastname: Chang
authors.affiliation: Department of Biotechnology and Animal Science, National Ilan University, Ilan 26041, Taiwan.
authors.forename: Tsun-Yung
authors.initials: TY
authors.lastname: Kuo
authors.affiliation: Department of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan. Electronic address: wbchung@mail.npust.edu.tw.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Chung
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000276:immunology; D060085:Coinfection / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D012333:RNA, Messenger; D051193:Toll-Like Receptors
keywords: Co-infection; Pigs; Porcine circovirus type 2; Porcine reproductive and respiratory syndrome virus; Toll-like receptors