title: Fast and reliable bacterial identification direct from positive blood culture using a new TFA sample preparation protocol and the Vitek® MS system.
abstract: A simple and reliable protocol using 0.1% trifluoroacetic acid (TFA) was developed to identify bacteria directly from blood cultures on the Vitek® MS system. Results presented a correlation of 99% for Gram-negative bacteria at species level, and 86.3% and 82.3% of Gram-positive bacteria at the genus and species levels, respectively.
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil. Electronic address: jussimara.nurmberger@afip.com.br.
authors.forename: Jussimara
authors.initials: J
authors.lastname: Monteiro
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
authors.forename: Fernanda Matsiko
authors.initials: FM
authors.lastname: Inoue
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
authors.forename: Ana Paula T
authors.initials: AP
authors.lastname: Lobo
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
authors.forename: Eduardo K
authors.initials: EK
authors.lastname: Sugawara
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
authors.forename: Fabiana M
authors.initials: FM
authors.lastname: Boaretti
authors.affiliation: Associação Fundo de Incentivo à Pesquisa, AFIP Medicina Diagnóstica, São Paulo, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Tufik
mesh_terms: D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014269:Trifluoroacetic Acid
chemical_list: D014269:Trifluoroacetic Acid
keywords: Bacterial identification; Blood culture; MALDI-TOF; Mass spectrometry; Trifluoroacetic acid; Vitek MS