title: The role of integrin-β/FAK in cyclic mechanical stimulation in MG-63 cells.
abstract: OBJECTIVE
  This study aims to explore the function of Integrin-β/FAK in the mechanical signal transduction and the connection with downstream ERK signal pathways.
  
  
  METHODS
  Human osteosarcoma MG63 cell lines were used in this study. The effects of mechanical strain on the Integrin-β₁ expression, FAK and ERK signal pathway in Human osteosarcoma MG63 cells were detected using RT-PCR and Western-blotting methods. The localization of FAK in Human osteosarcoma MG63 cells were determined using immunofluorescent method. The interaction between Integrin-β₁ and FAK were detected by using co-immunoprecipitation method.
  
  
  RESULTS
  The expression of Integrin-β₁ shows a notable bimodel distribution, mechanical strain stimulation can promote Integrin-β₁ expression and the phosphorylation of FAK and ERK, mechanical strain activated FAK and ERK mediated by Integrin-β₁.
  
  
  CONCLUSIONS
  Integrin-β₁ may play an important role in osteoblast proliferation differentiation process, it might feel external strain stimulation through ECM composition and makes FAK phosphated through the interaction with FAK, thus causing a series of activation of signal molecules. Finally it reduces MAPK (ERK) activation and cellular responses to finish mechanical signal transduction.
authors.affiliation: Institute of Endocrinology and Metabolism, The Second Xiangya Hospital, Central South University Changsha, P. R. China ; Department of Internal Medicine, Affiliated Hospital of National Research for Rehabilitation Technical Aids Beijing, P. R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Medical Center of Stomatology, The Second Xiangya Hospital, Central South University Changsha, P. R. China.
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Xiao
authors.affiliation: Institute of Endocrinology and Metabolism, The Second Xiangya Hospital, Central South University Changsha, P. R. China.
authors.forename: Er-Yuan
authors.initials: EY
authors.lastname: Liao
authors.affiliation: Medical Center of Stomatology, The Second Xiangya Hospital, Central South University Changsha, P. R. China.
authors.forename: Qiao-Jing
authors.initials: QJ
authors.lastname: Wang
authors.affiliation: Medical Center of Stomatology, The Second Xiangya Hospital, Central South University Changsha, P. R. China.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Wang
authors.affiliation: Medical Center of Stomatology, The Second Xiangya Hospital, Central South University Changsha, P. R. China.
authors.forename: Jia-Xuan
authors.initials: JX
authors.lastname: Lei
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D010766:Phosphorylation
chemical_list: D019012:Integrin beta1; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human
keywords: ERK signal pathway; Integrin-β; focal adhesion kinase (FAK); human osteosarcoma MG63 cells; mechanical stimulation