title: Critical role of platelet glycoprotein ibα in arterial remodeling.
abstract: OBJECTIVE
  Arteriogenesis is strongly dependent on the recruitment of leukocytes, especially monocytes, into the perivascular space of growing collateral vessels. On the basis of previous findings that platelets are central players in inflammatory processes and mediate the recruitment of leukocytes, the aim of this study was to assess the role of platelets in a model of arterial remodeling.
  
  
  RESULTS
  C57Bl6 wild-type mice, IL4-R/Iba mice lacking the extracellular domain of the glycoprotein Ibα (GPIbα) receptor, and mice treated with antibodies to block GPIbα or deplete circulating platelets were studied in peripheral arteriogenesis. Using a novel model of intravital 2-photon and epifluorescence imaging, we visualized and quantified the interaction of platelets with leukocytes and the vascular endothelium in vivo. We found that transient platelet adhesion to the endothelium of collateral vessels was a major event during arteriogenesis and depended on GPIbα. Furthermore, leukocyte recruitment was obviously affected in animals with defective platelet GPIbα function. In IL4-R/Iba mice, transient and firm leukocyte adhesion to the endothelium of collateral vessels, as well as leukocyte accumulation in the perivascular space, were significantly reduced. Furthermore, we detected platelet-leukocyte aggregates within the circulation, which were significantly reduced in IL4-R/Iba animals. Finally, platelet depletion and loss of GPIbα function resulted in poor reperfusion recovery as determined by laser Doppler imaging.
  
  
  CONCLUSIONS
  Thus, GPIbα-mediated interactions between platelets and endothelial cells, as well as leukocytes, support innate immune cell recruitment and promote arteriogenesis-establishing platelets as critical players in this process.
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Sue
authors.initials: S
authors.lastname: Chandraratne
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Marie-Luise
authors.initials: ML
authors.lastname: von Bruehl
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Judith-Irina
authors.initials: JI
authors.lastname: Pagel
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Konstantin
authors.initials: K
authors.lastname: Stark
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Eike
authors.initials: E
authors.lastname: Kleinert
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Ildiko
authors.initials: I
authors.lastname: Konrad
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Said
authors.initials: S
authors.lastname: Farschtschi
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Raffaele
authors.initials: R
authors.lastname: Coletti
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Florian
authors.initials: F
authors.lastname: Gärtner
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Omari
authors.initials: O
authors.lastname: Chillo
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Legate
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Michael
authors.initials: M
authors.lastname: Lorenz
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Simon
authors.initials: S
authors.lastname: Rutkowski
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Amelia
authors.initials: A
authors.lastname: Caballero-Martinez
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Richard
authors.initials: R
authors.lastname: Starke
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Anca
authors.initials: A
authors.lastname: Tirniceriu
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Laurenz
authors.initials: L
authors.lastname: Pauleikhoff
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Silvia
authors.initials: S
authors.lastname: Fischer
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Gerald
authors.initials: G
authors.lastname: Assmann
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Josef
authors.initials: J
authors.lastname: Mueller-Hoecker
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Jerry
authors.initials: J
authors.lastname: Ware
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Bernhard
authors.initials: B
authors.lastname: Nieswandt
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schaper
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Christian
authors.initials: C
authors.lastname: Schulz
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.).
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Deindl
authors.affiliation: From the Medizinische Klinik und Poliklinik I, Department of Cardiology (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M.), Walter-Brendel-Centre of Experimental Medicine (S.C., M.-L.v.B., J.-I.P., K.S., E.K., I.K., S.F., R.C., F.G., O.C., K.R.L., M.L., S.R., A.C.-M., A.T., L.P., C.S., E.D., S.M.), Department of Anaesthesiology (J.-I.P.), Department of Applied Physics (K.R.L.), and Institute of Pathology (G.A., J.M.-H.), Ludwig-Maximilians-Universität, Munich, Germany; DZHK (German Centre for Cardiovascular Research), Berlin, Germany (S.C., M.-L.v.B., K.S., I.K., S.F., R.C., F.G., K.R.L., M.L., A.T., C.S., S.M); Department of Biochemistry, Medical School, Justus-Liebig-University, Giessen, Germany (S.F.); Vascular Sciences, National Heart and Lung Institute, Faculty of Medicine, Hammersmith Campus, Imperial College London, South Kensington Campus, London, United Kingdom (R.S.); Department of Physiology and Biophysics, University of Arkansas for Medical Science, Little Rock (J.W.); Rudolf Virchow Center and DFG Research Center for Experimental Biomedicine, Universität Würzburg, Würzburg, Germany (B.N.); and Max Planck Institute for Heart and Lung Research, Giessen, Germany (W.S.). steffen.massberg@med.uni-muenchen.de.
authors.forename: Steffen
authors.initials: S
authors.lastname: Massberg
mesh_terms: D000818:Animals; D018919:Neovascularization, Physiologic; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*
chemical_list: D019038:Platelet Glycoprotein GPIb-IX Complex
keywords: adhesion receptor; blood platelets; leukocytes