title: Characterization of mariner-like transposons of the mauritiana Subfamily in seven tree aphid species.
abstract: Mariner-like elements (MLEs) are Class II transposons present in all eukaryotic genomes in which MLEs have been searched for. This article reports the detection of MLEs in seven of the main fruit tree aphid species out of eight species studied. Deleted MLE sequences of 916-919 bp were characterized, using the terminal-inverted repeats (TIRs) of mariner elements belonging to the mauritiana Subfamily as primers. All the sequences detected were deleted copies of full-length elements that included the 3'- and 5'-TIRs but displayed internal deletions affecting Mos1 activity. Networks based on the mtDNA cytochrome oxidase subunit-I (CO-I) and MLE sequences were incongruent, suggesting that mutations in transposon sequences had accumulated before speciation of tree aphid species occurred, and that they have been maintained in this species via vertical transmissions. This is the first evidence of the widespread occurrence of MLEs in aphids.
authors.affiliation: Faculté des Sciences de Tunis, Université de Tunis El Manar, UR11ES10 Génomique des insectes ravageurs, 2092, Manar II, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Kharrat
authors.forename: Maha
authors.initials: M
authors.lastname: Mezghani
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
authors.forename: Françoise
authors.initials: F
authors.lastname: Denis
authors.forename: Aurore
authors.initials: A
authors.lastname: Caruso
authors.forename: Hanem
authors.initials: H
authors.lastname: Makni
authors.forename: Pierre
authors.initials: P
authors.lastname: Capy
authors.forename: Jacques-Deric
authors.initials: JD
authors.lastname: Rouault
authors.forename: Benoît
authors.initials: B
authors.lastname: Chénais
authors.forename: Mohamed
authors.initials: M
authors.lastname: Makni
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids / Q000145:classification* / Q000235:genetics*; D001483:Base Sequence; D016000:Cluster Analysis; D004251:DNA Transposable Elements; D019143:Evolution, Molecular; D017344:Genes, Insect; D049750:Genome, Insect; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D004251:DNA Transposable Elements