title: [Influence of reagent storage in Sysmex CA7000 for different time on 4 test RESULTS: of the plasma coagulation].
abstract: The purpose of this study was to investigate the influence of blood coagulation reagents stored for different time on test results of the specimens prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (Fib). A total of 21 patient plasma specimens was taken and measured for homeostasis by Sysmex CA7000 automated blood coagulation analyzer and supporting reagent. The PT, APTT, TT and Fib of specimens were measured with the reagents stored in Sysmex CA7000 for different time. The differences of PT, APTT, TT and Fib were analyzed between values measured of the reagents stored for 0 hour and different time (TS:12, 24, 36,48, 60, 72 h; DA:24, 48, 72, 96, 120 h; TT:2, 4, 6, 8, 10, 12 h; TR:4, 8, 12, 16, 20, 24 h; OVB:1, 2, 3, 4, 5 ,6 h), respectively. The results showed that when coagulation reagent TS were stored for more than 48 h , DA 96 h, TT 10 h, TR 16 h and OVB 4 h, the values of PT, APTT, TT and Fib of samples were statistically different from the values measured with fresh coagulation reagent (P < 0.01), respectively. Compared 0 h with TS stored for 48-72, DA 96-120, TT 10-12, TR 16-24 and OVB 4-6 h, the percentage difference of PT, APTT, TT and Fib is in -2.6% ∼ 10.8%, -3.44% ∼ 4.8%, -3.9% ∼ 5.52%, -10.8% ∼ 3.3% and -17.2% ∼ 0.5%, the PT and Fib changes were more significant. Accordingly, the result of PT, APTT and TT had a uptrend as the reagent stored in Sysmex CA7000 analyzer for a long time, while Fib downtrend. It is concluded that the reagents showed be timely replaced when the plasma coagulation test is performed so as to obtain accurate results of examination.
authors.affiliation: Department of Blood Transfusion, Anhui Province Second People's Hospital, Hefei 230011,Anhui Province, China. E-mail:tongqingchen@126.com.
authors.forename: Tong-Qing
authors.initials: TQ
authors.lastname: Chen
authors.affiliation: Department of Blood Transfusion, Anhui Province Second People's Hospital, Hefei 230011,Anhui Province, China.
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Li
mesh_terms: D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D001793:Blood Preservation / Q000379:methods*; D005340:Fibrinogen; D006487:Hemostasis; D006801:Humans; D007202:Indicators and Reagents
chemical_list: D007202:Indicators and Reagents; D005340:Fibrinogen