title: A self-sustaining advanced lignocellulosic biofuel production by integration of anaerobic digestion and aerobic fungal fermentation.
abstract: High energy demand hinders the development and application of aerobic microbial biofuel production from lignocellulosic materials. In order to address this issue, this study focused on developing an integrated system including anaerobic digestion and aerobic fungal fermentation to convert corn stover, animal manure and food wastes into microbial lipids for biodiesel production. Dairy manure and food waste were first anaerobically digested to produce energy and solid digestate (AD fiber). AD fiber and corn stover were then processed by a combined alkali and acid hydrolysis, followed by fungal lipid accumulation. The integrated process can generate 1L biodiesel and 1.9 kg methane from 12.8 kg dry dairy manure, 3.1 kg dry food wastes and 12.2 kg dry corn stover with a positive net energy of 57 MJ, which concludes a self-sustaining lignocellulosic biodiesel process and provides a new route to co-utilize corn stover and organic wastes for advanced biofuel production.
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Ruan
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Yingkui
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Steven
authors.initials: S
authors.lastname: Archer
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. Electronic address: liaow@msu.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D050356:Lipid Metabolism; D008697:Methane / Q000737:chemistry; D013816:Thermodynamics; D014835:Volatilization; D003313:Zea mays / Q000737:chemistry
chemical_list: D056804:Biofuels; C036909:lignocellulose; D008031:Lignin; D008697:Methane
keywords: Anaerobic digestion; Biodiesel; Lignocellulosic material; Mortierella isabellina; Self-sustaining