title: Preventive effects of ¹²⁵I seeds on benign restenosis following esophageal stent implantation in a dog model.
abstract: The present study aimed to evaluate the effects of iodine‑125 (125I) seeds on the proliferation of primary esophageal fibroblasts in dogs, and to assess the safety and preventive efficacy of 125I seed‑pre‑loaded esophageal stents in benign restenosis following implantation. Primary fibroblasts were cultured with various 125I seed activities, which were then evaluated using cell proliferation and apoptosis assays as well as cell cycle analysis using Annexin V/propidium iodide (PI) double staining and PI staining. Prior to sacrification, animals were submitted to esophageal radiography under digital subtraction angiography. Esophageal tissues were collected and examined for macroscopic, microscopic and pathological alterations. The results demonstrated a significant and dose‑dependent inhibition of fibroblast proliferation and increased apoptosis following exposure to 125I seeds. G0/G1 fibroblast populations increased in a dose‑dependent manner following treatment with 125I seeds, in contrast to cells in S phase. Four weeks following implantation, α‑smooth muscle actin and proliferating cell nuclear antigen expression levels in the experimental group were significantly lower compared with those in the control group; in addition, eight weeks following implantation, esophageal inner diameters were increased in the experimental group. 125I seeds inhibited proliferation of dog esophageal fibroblasts via cell cycle arrest and apoptosis. In conclusion, 125I seed‑pre‑loaded esophageal stents inhibited benign hyperplasia in the upper edge of the stent to a certain extent, which relieved benign restenosis following implantation with a good safety profile.
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gan
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Jing
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Zhu
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Gaojun
authors.initials: G
authors.lastname: Teng
authors.affiliation: Department of Intervention and Vascular Surgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.
authors.forename: Jinhe
authors.initials: J
authors.lastname: Guo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002453:Cell Cycle / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D004195:Disease Models, Animal; D004285:Dogs; D004940:Esophageal Stenosis / Q000473:pathology / Q000517:prevention & control* / Q000601:surgery; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006909:Hydroxyproline / Q000378:metabolism; D007457:Iodine Radioisotopes; D008297:Male; D012008:Recurrence; D015607:Stents
chemical_list: D007457:Iodine Radioisotopes; D006909:Hydroxyproline