title: RNA Interference in Caenorhabditis elegans.
abstract: RNAi has become an essential tool in C. elegans research. This unit describes procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA, and soaking in dsRNA solution, as well as high-throughput methods for RNAi-based screens.
authors.affiliation: RNA Therapeutics Institute and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Darryl
authors.initials: D
authors.lastname: Conte
authors.affiliation: Programs in Systems Biology and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Lesley T
authors.initials: LT
authors.lastname: MacNeil
authors.affiliation: Programs in Systems Biology and Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Albertha J M
authors.initials: AJM
authors.lastname: Walhout
authors.affiliation: RNA Therapeutics Institute and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts.|Howard Hughes Medical Institute, Chevy Chase, Maryland.
authors.forename: Craig C
authors.initials: CC
authors.lastname: Mello
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods; D008967:Molecular Biology / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D034622:RNA Interference
keywords: C. elegans; RNAi; feeding; high-throughput screening assays; microinjection; soaking