title: Localization of resistin and its possible roles in the ovary of a vespertilionid bat, Scotophilus heathi.
abstract: The aim of the present study was to evaluate the expression and effect of resistin on ovarian activities of Scotophilus heathi. Immunohistochemical study showed marked variation in resistin immunostaining during different reproductive phases. Most intense immunostaining of resistin was noticed in thecal-interstitial cells in ovary during the period of delayed ovulation, the period of increased androgen synthesis and suppressed ovulation. The changes in ovarian resistin level also correlated positively with circulating leptin level and body white adipose tissue accumulation. The in vitro study showed that resistin alone preferentially stimulated progesterone synthesis, but with luteinizing hormone (LH) stimulated androgen secretion. Resistin alone dose-dependently increased expression of LH-receptor, steroidogenic acute regulatory protein and insulin receptor proteins in the ovary, whereas together with LH showed dose-dependent stimulatory effect on expression of androgen receptor and insulin receptor proteins in the ovary. In conclusion, during the period of fat accumulation increased ovarian resistin level may be responsible for increased androgen synthesis through insulin receptor mediated pathways in the ovary of S. heathi.
authors.affiliation: Reproductive Endocrinology Lab, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ajit
authors.initials: A
authors.lastname: Singh
authors.affiliation: Dr. Reddy's Institute of Life Sciences, Hyderabad University Campus, Gachibowly, Hyderabad 500046, India.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Suragani
authors.affiliation: Inflammation Biology and Cell Signaling Laboratory, National Institute of Pathology, Safdarjung Hospital Campus, New Delhi 110029, India.
authors.forename: Nasreen Z
authors.initials: NZ
authors.lastname: Ehtesham
authors.affiliation: Reproductive Endocrinology Lab, Department of Zoology, Banaras Hindu University, Varanasi 221005, India. Electronic address: akrishna_ak@yahoo.co.in.
authors.forename: Amitabh
authors.initials: A
authors.lastname: Krishna
mesh_terms: D052242:Adiponectin / Q000378:metabolism; D000728:Androgens / Q000097:blood; D000735:Androstenedione / Q000097:blood; D000818:Animals; D001835:Body Weight; D002685:Chiroptera / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000378:metabolism* / Q000502:physiology; D011972:Receptor, Insulin / Q000378:metabolism; D011974:Receptors, LH / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects; D052243:Resistin / Q000378:metabolism* / Q000494:pharmacology; D012621:Seasons
chemical_list: D052242:Adiponectin; D000728:Androgens; D007328:Insulin; D020738:Leptin; D011974:Receptors, LH; D052243:Resistin; D000735:Androstenedione; D007986:Luteinizing Hormone; D011972:Receptor, Insulin
keywords: A4; AR; IR; Insulin; LH-R; WAT