title: TMEM126A, a CD137 ligand binding protein, couples with the TLR4 signal transduction pathway in macrophages.
abstract: We showed previously that a novel protein, transmembrane protein 126A (TMEM126A), binds to CD137 ligand (CD137L, 4-1BBL) and couples with its reverse signals in macrophages. Here, we present data showing that TMEM126A relays TLR4 signaling. Thus, up-regulation of CD54 (ICAM-1), MHC II, CD86 and CD40 expression in response to TLR4 activation was diminished in TMEM126A-deficient macrophages. Moreover in TMEM126A-deficient RAW264.7 cells, LPS/TLR4-induced late-phase JNK/SAPK and IRF-3 phosphorylation was abolished. These findings indicate that TMEM126A contributes to the TLR4 signal up-regulating the expression of genes whose products are involved in antigen presentation.
authors.affiliation: Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
authors.affiliation: Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Ji-Hoi
authors.initials: JH
authors.lastname: Moon
authors.affiliation: School of Biological Sciences, University of Ulsan, Ulsan 680-749, Republic of Korea.
authors.forename: Sang W
authors.initials: SW
authors.lastname: Kang
authors.affiliation: School of Biological Sciences, University of Ulsan, Ulsan 680-749, Republic of Korea.
authors.forename: Byungsuk
authors.initials: B
authors.lastname: Kwon
authors.affiliation: Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea. Electronic address: hyeonwoo@khu.ac.kr.
authors.forename: Hyeon-Woo
authors.initials: HW
authors.lastname: Lee
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D019013:CD40 Antigens / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D005786:Gene Expression Regulation; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D050838:Interferon Regulatory Factor-3 / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D048670:MAP Kinase Kinase 4 / Q000235:genetics / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D061251:Primary Cell Culture; D011485:Protein Binding; D015398:Signal Transduction / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000276:immunology; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000235:genetics* / Q000276:immunology
chemical_list: D051940:B7-2 Antigen; D019013:CD40 Antigens; D000949:Histocompatibility Antigens Class II; D050838:Interferon Regulatory Factor-3; C494233:Irf3 protein, mouse; D008070:Lipopolysaccharides; D008565:Membrane Proteins; C495345:TLR4 protein, human; C578692:TMEM126A protein, mouse; D051197:Toll-Like Receptor 4; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D018799:Intercellular Adhesion Molecule-1; D048670:MAP Kinase Kinase 4
keywords: Antigen presenting; CD137L; Innate immunity; TMEM126A; TRL4