title: [Genetic characterization of the Wad Medani virus (WMV) (Reoviridae, Orbivirus), isolated from the ticks Hyalomma asiaticum Schulze et Schlottke, 1930 (Ixodidae: Hyalomminae) in Turkmenistan, Kazakhstan, and Armenia and from the ticks H. anatolicum Koch, 1844 in Tajikistan].
abstract: Near full-genome sequence of the Wad Medani Virus (WMV) (strain LEIV-8066Tur) (Orbivirus, Reoviridae) isolated from the ticks Hyalomma asiaticum Schulze et Schlottke, 1929, collected from sheep in Baharly district in Turkmenistan, was determined using next generation sequencing approach. The similarity of the RNA-dependent RNA-polymerase (Pol, VP1) amino acid sequence between WMV and the Kemerovo group orbiviruses (KEMV), as well as of the Baku virus (BAKV), was 64%. The similarity of the conserved structural protein VP3 (T2) of WMV with mosquito-borne and tick-borne orbiviruses reaches 46% and 67%, respectively. For the surface proteins VP2, VP5, and VP7 (T13), which have major antigenic determinants of orbiviruses, the similarity of WMV with tickborne orbiviruses (KEMV and BAKV) is 26-30%, 45% and, 57%, respectively (ID GenBank: KJ425426-35).
authors.forename: S V
authors.initials: SV
authors.lastname: Al'khovskiĭ
authors.forename: D K
authors.initials: DK
authors.lastname: L'vov
authors.forename: M Iu
authors.initials: MIu
authors.lastname: Shchelkanov
authors.forename: A M
authors.initials: AM
authors.lastname: Shchetinin
authors.forename: P G
authors.initials: PG
authors.lastname: Deriabin
authors.forename: A K
authors.initials: AK
authors.lastname: Gitel'man
authors.forename: V A
authors.initials: VA
authors.lastname: Aristova
authors.forename: A G
authors.initials: AG
authors.lastname: Botikov
mesh_terms: D000818:Animals; D001136:Armenia; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D026863:Ixodidae / Q000821:virology; D007623:Kazakhstan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012087:Reoviridae / Q000235:genetics* / Q000472:pathogenicity; D012088:Reoviridae Infections / Q000235:genetics / Q000821:virology*; D012756:Sheep / Q000821:virology; D013620:Tajikistan; D014423:Turkmenistan