title: GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes.
abstract: BACKGROUND
  In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal-epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124-131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells.
  
  
  METHODS
  Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line.
  
  
  RESULTS
  We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells.
  
  
  CONCLUSIONS
  Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity.
authors.affiliation: Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Universidad de Buenos Aires-IQUIBICEN, CONICET, Intendente Güiraldes 2160, Pabellón 2, 1428, Buenos Aires, Argentina, liliana@qb.fcen.uba.ar.
authors.forename: L N
authors.initials: LN
authors.lastname: Guerra
authors.forename: C
authors.initials: C
authors.lastname: Suarez
authors.forename: D
authors.initials: D
authors.lastname: Soto
authors.forename: A
authors.initials: A
authors.lastname: Schiappacasse
authors.forename: D
authors.initials: D
authors.lastname: Sapochnik
authors.forename: P
authors.initials: P
authors.lastname: Sacca
authors.forename: G
authors.initials: G
authors.lastname: Piwien-Pilipuk
authors.forename: B
authors.initials: B
authors.lastname: Peral
authors.forename: J C
authors.initials: JC
authors.lastname: Calvo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000378:metabolism; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D041681:NIH 3T3 Cells; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015238:Sulfotransferases / Q000378:metabolism*; D013466:Sulfurtransferases / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; C493411:Fabp4 protein, mouse; D050556:Fatty Acid-Binding Proteins; D014280:Triglycerides; D013466:Sulfurtransferases; C432419:GAL3ST2 protein, human; C506605:Gal3St2 protein, mouse; D015238:Sulfotransferases