title: Association of VPg and eIF4E in the host tropism at the cellular level of Barley yellow mosaic virus and Wheat yellow mosaic virus in the genus Bymovirus.
abstract: Barley yellow mosaic virus (BaYMV) and Wheat yellow mosaic virus (WYMV) are separate species in the genus Bymovirus with bipartite plus-sense RNA genomes. In fields, BaYMV infects only barley and WYMV infects only wheat. Here, we studied the replicative capability of the two viruses in barley and wheat mesophyll protoplasts. BaYMV replicated in both barley and wheat protoplasts, but WYMV replicated only in wheat protoplasts. The expression of wheat translation initiation factor 4E (eIF4E), a common host factor for potyviruses, from the WYMV genome enabled WYMV replication in barley protoplasts. Replacing the BaYMV VPg gene with that of WYMV abolished BaYMV replication in barley protoplasts, whereas the additional expression of wheat eIF4E from BaYMV genome restored the replication of the BaYMV mutant in barley protoplasts. These results indicate that both VPg and the host eIF4E are involved in the host tropism of BaYMV and WYMV at the replication level.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
authors.forename: Huangai
authors.initials: H
authors.lastname: Li
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Asian Natural Environmental Science Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: yshirako@anesc.u-tokyo.ac.jp.
authors.forename: Yukio
authors.initials: Y
authors.lastname: Shirako
mesh_terms: D039561:Eukaryotic Initiation Factor-4E / Q000235:genetics / Q000378:metabolism*; D001467:Hordeum / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D058507:Host Specificity; D010935:Plant Diseases / Q000821:virology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D019176:Potyviridae / Q000235:genetics / Q000502:physiology*; D011485:Protein Binding; D014908:Triticum / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D056189:Viral Tropism; D014779:Virus Replication
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D010940:Plant Proteins; D014764:Viral Proteins
keywords: Barley yellow mosaic virus (BaYMV); Bymovirus; Eukaryotic translation initiation factor 4E (eIF4E); Mesophyll protoplasts; Replication; Viral genome-linked protein (VPg); Wheat yellow mosaic virus (WYMV)