title: Clinical response and transfusion reactions of sheep subjected to single homologous blood transfusion.
abstract: Studies in relation to blood conservation and responses to transfusion are scarce for ruminants. We evaluated the clinical manifestations of sheep that received a single homologous transfusion of whole blood, focusing on transfusion reactions. Eighteen adult sheep were subjected to a single phlebotomy to withdraw 40% of the total blood volume, which was placed into CPDA-1 bags and then divided into G0, animals that received fresh blood, and G15 and G35, animals that received blood stored for 15 or 35 days, respectively. Clinical observations were recorded throughout the transfusion, whereas heart rate, respiratory rate, and rectal temperature were assessed at the following times: 24 hours after phlebotomy and before transfusion; 30 minutes, six, twelve, 24, 48, 72, and 96 hours and eight and 16 days after transfusion. All groups presented transfusion reactions, among which hyperthermia was the most frequent (50% of animals). Tachycardia occurred most frequently in the G35 animals (50% of them). During transfusion G35 animals presented more clinical manifestation (P < 0.05). Transfusion of fresh or stored total blood improved the blood volume, but transfusion reactions occurred, demonstrating that a single transfusion of fresh or stored blood can cause inflammatory and febrile nonhemolytic transfusion reactions in sheep.
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Rejane Santos
authors.initials: RS
authors.lastname: Sousa
authors.affiliation: Institute of Biodiversity and Forest, Federal University of Western Pará, Avenida Vera Paz S/N, Salé, 68000-000 Santarém, PA, Brazil.
authors.forename: Antonio Humberto Hamad
authors.initials: AH
authors.lastname: Minervino
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Carolina Akiko Sato Cabral
authors.initials: CA
authors.lastname: Araújo
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Frederico Augusto Mazzocca Lopes
authors.initials: FA
authors.lastname: Rodrigues
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Francisco Leonardo Costa
authors.initials: FL
authors.lastname: Oliveira
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Clara Satsuki
authors.identifier: 0000-0002-4897-7764
authors.initials: CS
authors.lastname: Mori
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Janaina Larissa Rodrigues
authors.initials: JL
authors.lastname: Zaminhan
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Thiago Rocha
authors.initials: TR
authors.lastname: Moreira
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Isadora Karolina Freitas
authors.initials: IK
authors.lastname: Sousa
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, University of Sao Paulo, Avenida Professor Orlando Marques de Paiva, 87 Cidade Universitária, 05508-270 São Paulo, SP, Brazil.
authors.forename: Enrico Lippi
authors.initials: EL
authors.lastname: Ortolani
authors.affiliation: Department of Animal Science, Federal Rural University of the Semiarid Region, Avenida Francisco Mota, s/n Bairro Presidente Costa e Silva, 59625-900 Mossoró, RN, Brazil.
authors.forename: Raimundo Alves
authors.initials: RA
authors.lastname: Barrêto
mesh_terms: D000818:Animals; D001793:Blood Preservation; D001803:Blood Transfusion; D012756:Sheep; D013997:Time Factors; D065227:Transfusion Reaction / Q000378:metabolism* / Q000473:pathology*