title: β-(1 → 3)-Glucanolytic yeasts from Brazilian grape microbiota: production and characterization of β-glucanolytic enzymes by Aureobasidium pullulans 1WA1 cultivated on fungal Mycelium.
abstract: A total of 95 yeast strains were isolated from the microbiota of different grapes collected at vineyards in southern Brazil. The yeasts were screened for β-(1 → 3)-glucanases using a newly developed zymogram method that relies upon the appearance of clearance zones around growing colonies cultured on agar–botryosphaeran medium and also by submerged fermentation on nutrient medium containing botryosphaeran, a (1 → 3),(1 → 6)-β-d-glucan. Among 14 β-(1 → 3)-glucanase-positive yeasts identified, four strains produced the highest β-glucanolytic activities and were evaluated for enzyme production on cellobiose, botryosphaeran, and mycelial biomass from Botryosphaeria rhodina (MAMB-05). Yeast strain 1WA1 produced the highest β-(1 → 3)-glucanase and β-glucosidase activities and was identified by molecular characterization as Aureobasidium pullulans. The physicochemical properties of the crude β-glucanolytic enzyme preparation were characterized, and the preparation was used to hydrolyze several β-d-glucans (laminarin, botryosphaeran, lasiodiplodan, pustulan, and curdlan). The production and physicochemical properties of the β-glucanolytic preparation enable its potential applications in wine enology and production of prebiotics through hydrolysis of β-d-glucans.
authors.forename: Anelize
authors.initials: A
authors.lastname: Bauermeister
authors.forename: Ismael R
authors.initials: IR
authors.lastname: Amador
authors.forename: Carla P
authors.initials: CP
authors.lastname: Pretti
authors.forename: Ellen C
authors.initials: EC
authors.lastname: Giese
authors.forename: André L M
authors.initials: AL
authors.lastname: Oliveira
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001938:Brazil; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D064307:Microbiota; D025282:Mycelium / Q000737:chemistry / Q000201:enzymology / Q000235:genetics; D013379:Substrate Specificity; D027843:Vitis / Q000382:microbiology*; D015003:Yeasts / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D047071:beta-Glucans; D006026:Glycoside Hydrolases