title: Using Human Induced Pluripotent Stem Cells to Model Skeletal Diseases.
abstract: Musculoskeletal disorders affecting the bones and joints are major health problems among children and adults. Major challenges such as the genetic origins or poor diagnostics of severe skeletal disease hinder our understanding of human skeletal diseases. The recent advent of human induced pluripotent stem cells (human iPS cells) provides an unparalleled opportunity to create human-specific models of human skeletal diseases. iPS cells have the ability to self-renew, allowing us to obtain large amounts of starting material, and have the potential to differentiate into any cell types in the body. In addition, they can carry one or more mutations responsible for the disease of interest or be genetically corrected to create isogenic controls. Our work has focused on modeling rare musculoskeletal disorders including fibrodysplasia ossificans progressive (FOP), a congenital disease of increased heterotopic ossification. In this review, we will discuss our experiences and protocols differentiating human iPS cells toward the osteogenic lineage and their application to model skeletal diseases. A number of critical challenges and exciting new approaches are also discussed, which will allow the skeletal biology field to harness the potential of human iPS cells as a critical model system for understanding diseases of abnormal skeletal formation and bone regeneration.
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine, Institute for Human Genetics, University of California, 513 Parnassus Ave., HSE901G, San Francisco, CA, 94143-0794, USA.
authors.forename: Emilie
authors.initials: E
authors.lastname: Barruet
authors.affiliation: Division of Endocrinology and Metabolism, Department of Medicine, Institute for Human Genetics, University of California, 513 Parnassus Ave., HSE901G, San Francisco, CA, 94143-0794, USA. Edward.hsiao@ucsf.edu.
authors.forename: Edward C
authors.initials: EC
authors.lastname: Hsiao
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D061252:Feeder Cells / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008954:Models, Biological; D009221:Myositis Ossificans / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009999:Ossification, Heterotopic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D061251:Primary Cell Culture; D012692:Serial Passage
chemical_list: D015415:Biomarkers; D000469:Alkaline Phosphatase
keywords: Directed differentiation; Fibrodysplasia ossificans progressiva; Human iPS cells; Mineralization; Ossification; Skeletal diseases