title: Evaluation of a murine single-blood-injection SAH model.
abstract: The molecular pathways underlying the pathogenesis after subarachnoid haemorrhage (SAH) are poorly understood and continue to be a matter of debate. A valid murine SAH injection model is not yet available but would be the prerequisite for further transgenic studies assessing the mechanisms following SAH. Using the murine single injection model, we examined the effects of SAH on regional cerebral blood flow (rCBF) in the somatosensory (S1) and cerebellar cortex, neuro-behavioural and morphological integrity and changes in quantitative electrocorticographic and electrocardiographic parameters. Micro CT imaging verified successful blood delivery into the cisterna magna. An acute impairment of rCBF was observed immediately after injection in the SAH and after 6, 12 and 24 hours in the S1 and 6 and 12 hours after SAH in the cerebellum. Injection of blood into the foramen magnum reduced telemetric recorded total ECoG power by an average of 65%. Spectral analysis of ECoGs revealed significantly increased absolute delta power, i.e., slowing, cortical depolarisations and changes in ripples and fast ripple oscillations 12 hours and 24 hours after SAH. Therefore, murine single-blood-injection SAH model is suitable for pathophysiological and further molecular analysis following SAH.
authors.affiliation: Department for Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Moorenstraße 5, D-40225 Düsseldorf, Germany; Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, D-50931 Köln, Germany.
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Kamp
authors.affiliation: Department for Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Moorenstraße 5, D-40225 Düsseldorf, Germany; Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, D-50931 Köln, Germany; Center of Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, D-50931 Köln, Germany.
authors.forename: Maxine
authors.initials: M
authors.lastname: Dibué
authors.affiliation: Department for Neuropathology, University Medical Center of the Johannes Gutenberg University, Langenbeckstr. 1, D-55131 Mainz, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Sommer
authors.affiliation: Department for Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Moorenstraße 5, D-40225 Düsseldorf, Germany.
authors.forename: Hans-Jakob
authors.initials: HJ
authors.lastname: Steiger
authors.affiliation: Institute for Neurophysiology, Medical Faculty, University of Cologne, Robert-Koch-Str. 39, D-50931 Köln, Germany; Center of Molecular Medicine Cologne (CMMC), Robert-Koch-Str. 39, D-50931 Köln, Germany.
authors.forename: Toni
authors.initials: T
authors.lastname: Schneider
authors.affiliation: Department for Neurosurgery, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Moorenstraße 5, D-40225 Düsseldorf, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hänggi
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000098:blood supply* / Q000503:physiopathology; D002946:Cisterna Magna; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D012039:Regional Blood Flow; D013003:Somatosensory Cortex / Q000098:blood supply* / Q000503:physiopathology; D013345:Subarachnoid Hemorrhage / Q000503:physiopathology*