title: The evidence of Tobacco rattle virus impact on host plant organelles ultrastructure.
abstract: Tobraviruses, like other (+) stranded RNA viruses of plants, replicate their genome in cytoplasm and use such usual membranous structures like endoplasmic reticulum. Based on the ultrastructural examination of Tobacco rattle virus (TRV)-infected potato and tobacco leaf tissues, in this work we provide evidence of the participation of not only the membranous and vesicular ER structures but also other cell organelles during the viral infection cycle. Non-capsidated TRV PSG particles (potato isolate from the Netherlands) (long and short forms) were observed inside the nucleus while the presence of TRV capsid protein (CP) was detected in the nucleus caryolymph and within the nucleolus area. Both capsidated and non-capsidated viral particles were localized inside the strongly disorganized chloroplasts and mitochondria. The electron-dense TRV particles were connected with vesicular structures of mitochondria as well as with chloroplasts in both potato and tobacco tissues. At 15-30 days after infection, vesicles filled with TRV short particles were visible in mitochondria revealing the expanded cristae structures. Immunodetection analysis revealed the TRV PSG CP epitope inside chloroplast with disorganized thylakoids structure as well as in mitochondria of different tobacco and potato tissues. The ultrastructural analysis demonstrated high dynamics of the main cell organelles during the TRV PSG-Solanaceous plants interactions. Moreover, our results suggest a relationship between organelle changes and different stages of virus infection cycle and/or particle formation.
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, WULS-SGGW, Nowoursynowska Str. 159, 02-776 Warsaw, Poland. Electronic address: katarzyna_otulak@sggw.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Otulak
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, WULS-SGGW, Nowoursynowska Str. 159, 02-776 Warsaw, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Chouda
authors.affiliation: Plant Molecular Biology Center and the Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA; Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61-704 Poznan, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Bujarski
authors.affiliation: Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences, WULS-SGGW, Nowoursynowska Str. 159, 02-776 Warsaw, Poland.
authors.forename: Grażyna
authors.initials: G
authors.lastname: Garbaczewska
mesh_terms: D036022:Capsid Proteins / Q000302:isolation & purification; D002467:Cell Nucleus / Q000648:ultrastructure / Q000821:virology; D002736:Chloroplasts / Q000648:ultrastructure / Q000821:virology; D004721:Endoplasmic Reticulum / Q000648:ultrastructure* / Q000821:virology; D058503:Mesophyll Cells / Q000648:ultrastructure / Q000821:virology; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000648:ultrastructure / Q000821:virology; D015388:Organelles / Q000648:ultrastructure* / Q000821:virology*; D052585:Phloem / Q000648:ultrastructure / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000502:physiology* / Q000648:ultrastructure; D012328:RNA Viruses / Q000502:physiology* / Q000648:ultrastructure; D011198:Solanum tuberosum / Q000821:virology; D014026:Nicotiana / Q000821:virology
chemical_list: D036022:Capsid Proteins
keywords: Chloroplast; Endoplasmic reticulum; Mitochondria; Nucleus; Plant–virus interaction; Tobacco rattle virus