title: Novel sandwich immunoassays for the measurement of total and active FGF21.
abstract: BACKGROUND
  FGF21 is involved in glucose and lipid metabolism. Our objective was to develop two novel sandwich immunoassays that measure active and total levels of FGF21 in human plasma.
  
  
  RESULTS
  Both immunoassays utilized affinity-optimized monoclonal antibodies generated specifically for either the mid-domain or the intact C-terminus of the respective FGF21 peptides. Total FGF21 levels measured from normal human plasma samples ranged from 42 to 462 pg/ml, while active FGF21 levels ranged from 11 to 399 pg/ml. The data also suggested no significant differences in the concentrations of active FGF21 when measured from pre- and post-prandial samples.
  
  
  CONCLUSIONS
  We have successfully developed sensitive assays to measure active and total FGF21, which show the majority of total FGF21 in plasma is active FGF21.
authors.affiliation: Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA.
authors.forename: Tara S
authors.initials: TS
authors.lastname: Umberger
authors.forename: John H
authors.initials: JH
authors.lastname: Sloan
authors.forename: Jinbiao
authors.initials: J
authors.lastname: Chen
authors.forename: Christine
authors.initials: C
authors.lastname: Cheng
authors.forename: Robert W
authors.initials: RW
authors.lastname: Siegel
authors.forename: Yuewei
authors.initials: Y
authors.lastname: Qian
authors.forename: Jason S
authors.initials: JS
authors.lastname: Troutt
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Konrad
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D005260:Female; D005346:Fibroblast Growth Factors / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors