title: Microfluidic method of pig oocyte quality assessment in relation to different follicular size based on lab-on-chip technology.
abstract: Since microfollicular environment and the size of the follicle are important markers influencing oocyte quality, the aim of this study is to present the spectral characterization of oocytes isolated from follicles of various sizes using lab-on-chip (LOC) technology and to demonstrate how follicle size may affect oocyte quality. Porcine oocytes (each, n = 100) recovered from follicles of different sizes, for example, from large (>5 mm), medium (3-5 mm), and small (<3 mm), were analyzed after preceding in vitro maturation (IVM). The LOC analysis was performed using a silicon-glass sandwich with two glass optical fibers positioned "face-to-face." Oocytes collected from follicles of different size classes revealed specific and distinguishable spectral characteristics. The absorbance spectra (microspectrometric specificity) for oocytes isolated from large, medium, and small follicles differ significantly (P < 0.05) and the absorbance wavelengths were between 626 and 628 nm, between 618 and 620 nm, and less than 618 nm, respectively. The present study offers a parametric and objective method of porcine oocyte assessment. However, up to now this study has been used to evidence spectral markers associated with follicular size in pigs, only. Further investigations with functional-biological assays and comparing LOC analyses with fertilization and pregnancy success and the outcome of healthy offspring must be performed.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60-781 Poznan, Poland ; Department of Anatomy, Poznan University of Medical Science, 6 Swiecickiego Street, 60-781 Poznan, Poland.
authors.forename: Bartosz
authors.identifier: 0000-0002-6423-686X
authors.initials: B
authors.lastname: Kempisty
authors.affiliation: Department of Microengineering and Photovoltaics, Wroclaw University of Technology, 11/17 Janiszewskiego Street, 50-372 Wroclaw, Poland.
authors.forename: Rafał
authors.initials: R
authors.lastname: Walczak
authors.affiliation: Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Antosik
authors.affiliation: Department of Microengineering and Photovoltaics, Wroclaw University of Technology, 11/17 Janiszewskiego Street, 50-372 Wroclaw, Poland.
authors.forename: Patrycja
authors.initials: P
authors.lastname: Sniadek
authors.affiliation: Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, Poland.
authors.forename: Marta
authors.identifier: 0000-0001-9717-5250
authors.initials: M
authors.lastname: Rybska
authors.affiliation: Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Street, 60-631 Poznan, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Piotrowska
authors.affiliation: Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Bukowska
authors.affiliation: Department of Anatomy, Poznan University of Medical Science, 6 Swiecickiego Street, 60-781 Poznan, Poland.
authors.forename: Jan
authors.initials: J
authors.lastname: Dziuban
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecickiego Street, 60-781 Poznan, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Institute of Veterinary Sciences, Poznan University of Life Sciences, 52 Wojska Polskiego Street, 60-628 Poznan, Poland.
authors.forename: Jędrzej M
authors.initials: JM
authors.lastname: Jaśkowski
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, 6a Chalubinskiego Street, 50-368 Wroclaw, Poland.
authors.forename: Maciej
authors.identifier: 0000-0003-2004-0010
authors.initials: M
authors.lastname: Zabel
authors.affiliation: Department of Experimental Reproductive Biology, Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Brüssow
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D059471:In Vitro Oocyte Maturation Techniques; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D009865:Oocytes / Q000254:growth & development*; D006080:Ovarian Follicle / Q000254:growth & development*; D011247:Pregnancy; D013552:Swine