title: Up-regulated 14-3-3β and 14-3-3ζ proteins in prefrontal cortex of subjects with schizophrenia: effect of psychotropic treatment.
abstract: 14-3-3 is a family of conserved regulatory proteins that bind to a multitude of functionally diverse signalling proteins. Various genetic studies and gene expression and proteomic analyses have involved 14-3-3 proteins in schizophrenia (SZ). On the other hand, studies about the status of these proteins in major depressive disorder (MD) are still missing. Immunoreactivity values of cytosolic 14-3-3β and 14-3-3ζ proteins were evaluated by Western blot in prefrontal cortex (PFC) of subjects with schizophrenia (SZ; n=22), subjects with major depressive disorder (MD; n=21) and age-, gender- and postmortem delay-matched control subjects (n=52). The modulation of 14-3-3β and 14-3-3ζ proteins by psychotropic medication was also assessed. The analysis of both proteins in SZ subjects with respect to matched control subjects showed increased 14-3-3β (Δ=33±10%, p<0.05) and 14-3-3ζ (Δ=29±6%, p<0.05) immunoreactivity in antipsychotic-free but not in antipsychotic-treated SZ subjects. Immunoreactivity values of 14-3-3β and 14-3-3ζ were not altered in MD subjects. These results show the specific up-regulation of 14-3-3β and 14-3-3ζ proteins in PFC of SZ subjects and suggest a possible down-regulation of both proteins by antipsychotic treatment.
authors.affiliation: Department of Pharmacology, University of te Basque Country (UPV/EHU), Leioa, Bizkaia, Spain; BioCruces Health Research Institute and Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Spain. Electronic address: guadalupe.rivero@ehu.es.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Rivero
authors.affiliation: Department of Pharmacology, University of te Basque Country (UPV/EHU), Leioa, Bizkaia, Spain; BioCruces Health Research Institute and Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Spain.
authors.forename: Ane M
authors.initials: AM
authors.lastname: Gabilondo
authors.affiliation: Laboratory of Neuropharmacology, IUNICS-IdISPa, University of the Balearic Islands (UIB), and Redes Temáticas de Investigación Cooperativa en Salud-Red de Trastornos Adictivos (RETICS-RTA), Spain.
authors.forename: Jesús A
authors.initials: JA
authors.lastname: García-Sevilla
authors.affiliation: Centre Universitaire Romand de Médecine Légale-Site Genève, University of Geneva, Switzerland.
authors.forename: Romano
authors.initials: R
authors.lastname: La Harpe
authors.affiliation: Section of Forensic Pathology, Basque Institute of Legal Medicine, Bilbao, Spain.
authors.forename: Benito
authors.initials: B
authors.lastname: Morentín
authors.affiliation: Department of Pharmacology, University of te Basque Country (UPV/EHU), Leioa, Bizkaia, Spain; BioCruces Health Research Institute and Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Spain.
authors.forename: J Javier
authors.initials: JJ
authors.lastname: Meana
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism*; D000328:Adult; D000928:Antidepressive Agents / Q000627:therapeutic use; D014150:Antipsychotic Agents / Q000627:therapeutic use*; D015153:Blotting, Western; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003865:Major Depressive Disorder / Q000188:drug therapy / Q000378:metabolism; D005260:Female; D016591:Histocytological Preparation Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism*; D012559:Schizophrenia / Q000188:drug therapy* / Q000378:metabolism*; D012727:Sex Characteristics; D013997:Time Factors
chemical_list: D048948:14-3-3 Proteins; D000928:Antidepressive Agents; D014150:Antipsychotic Agents
keywords: 14-3-3 proteins; Western blot; human prefrontal cortex; major depression; postmortem; schizophrenia