title: Redescription and molecular characterisation of Dujardinascaris madagascariensis and a note on D. dujardini (Nematoda: Heterocheilidae), parasites of Crocodylus niloticus, with a key to Dujardinascaris spp. in crocodilians.
abstract: An examination of one specimen of Nile crocodile, Crocodylus niloticus (Laurenti, 1768), from Lake Turkana (Kenya), revealed the presence of two ascaridoid nematodes belonging to the genus Dujardinascaris Baylis, 1947. Dujardinascaris madagascariensis Chabaud & Caballero, 1966 was studied by scanning electron microscopy, redescribed, and differentiated from D. dujardini (Travassos, 1920). Dujardinascaris madagascariencsis is the second of the genus to be sequenced. An internal fragment of the small ribosomal subunit and nuclear ribosomal DNA internal transcribed spacer 2 region were amplified--the slowly evolving 18S gene region was used for phylogenetic analysis. Molecular data confirmed affinity of D. madagascariensis to the family Heterocheilidae and revealed its closest relationship with D. waltoni. A key to the species of Dujardinascaris parasitizing crocodiles is provided.
authors.affiliation: Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.; Email: masova@sci.muni.cz.
authors.forename: Šárka
authors.initials: Š
authors.lastname: Mašová
authors.affiliation: Deceased; Email: unknown.
authors.forename: Vlastimil
authors.initials: V
authors.lastname: Baruš
authors.affiliation: Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic.; Email: unknown.
authors.forename: Mária
authors.initials: M
authors.lastname: Seifertová
authors.affiliation: Kenya Marine and Fisheries Research Institute, Lake Turkana Station, P. O. Box 205, Kalokol, Kenya; Email: unknown.
authors.forename: John
authors.initials: J
authors.lastname: Malala
authors.affiliation: Biology Centre of the Academy of Sciences of the Czech Republic, Institute of Parasitology, Branišovská 31, 370 05 České Budějovice, Czech Republic; Email: unknown.
authors.forename: Miloslav
authors.initials: M
authors.lastname: Jirků
mesh_terms: D000489:Alligators and Crocodiles / Q000469:parasitology*; D063147:Animal Distribution; D000825:Animal Structures / Q000033:anatomy & histology / Q000254:growth & development; D000818:Animals; D049628:Body Size; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005260:Female; D007630:Kenya; D008297:Male; D009348:Nematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D009349:Nematode Infections / Q000469:parasitology / Q000662:veterinary*; D009929:Organ Size; D010802:Phylogeny
chemical_list: D021903:DNA, Ribosomal Spacer