title: Fibrinogen adsorption and platelet adhesion to silica surfaces with stochastic nanotopography.
abstract: In this study, the effect of surface nanoscale roughness on fibrinogen adsorption and platelet adhesion was investigated. Nanorough silica surfaces with a low level of surface roughness (10 nm Rrms) were found to support the same level of fibrinogen adsorption as the planar silica surfaces, while nanorough silica surfaces with higher levels of surface roughness (15 nm Rrms) were found to support significantly less fibrinogen adsorption. All surfaces analyzed were found to support the same level of platelet adhesion; however, platelets were rounded in morphology on the nanorough silica surfaces while platelets were spread with a well-developed actin cytoskeleton on the planar silica. Unique quartz crystal microbalance with dissipation monitoring (QCM-D) responses was observed for the interactions between platelets and each of the surfaces. The QCM-D data indicated that platelets were more weakly attached to the nanorough silica surfaces compared with the planar silica. These data support the role of surface nanotopography in directing platelet-surface interactions even when the adsorbed fibrinogen layer is able to support the same level of platelet adhesion.
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia.
authors.forename: Megan S
authors.initials: MS
authors.lastname: Lord
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia.
authors.forename: John M
authors.initials: JM
authors.lastname: Whitelock
authors.affiliation: School of Mechanical and Manufacturing Engineering, University of New South Wales, Sydney, New South Wales 2052, Australia.
authors.forename: Anne
authors.initials: A
authors.lastname: Simmons
authors.affiliation: Department of Eye and Vision Science, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L69 3GA, United Kingdom.
authors.forename: Rachel L
authors.initials: RL
authors.lastname: Williams
authors.affiliation: Faculty of Science, University of Technology, Sydney, Broadway, New South Wales 2007, Australia.
authors.forename: Bruce K
authors.initials: BK
authors.lastname: Milthorpe
mesh_terms: D000327:Adsorption; D001792:Blood Platelets / Q000166:cytology / Q000502:physiology*; D002448:Cell Adhesion; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D012822:Silicon Dioxide; D005340:Fibrinogen