title: Biosynthesis, characterization, and hemostasis potential of tailor-made poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced by Haloferax mediterranei.
abstract: We report the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) random copolymers (R-PHBV) or higher-order copolymers (O-PHBV) in Haloferax mediterranei, with adjustable 3-hydroxyvalerate (3HV) incorporation by cofeeding valerate with glucose. Their microchemical structure, molecular weight and its distribution, and thermal and mechanical properties were characterized by NMR, GPC, DSC, TGA, and universal testing machine, respectively. (13)C NMR studies showed that O-PHBV copolymers consisted of short segments of PHB and PHV covalently linked together with random PHBV segments. Consistently, two Tg were observed in the DSC curves of O-PHBV. The "blocky" feature of O-PHBV enhanced crystallinity percentages and improved Young's modulus. Notably, the film of one O-PHBV copolymer, O-PHBV-1, showed unique foveolar cluster-like surface morphology with high hydrophobicity and roughness, as characterized using static contact angle and SEM and AFM analyses. It also exhibited increased platelet adhesion and accelerated blood clotting. The excellent hemostatic properties endow this copolymer with great potential in wound healing.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
authors.forename: Dahe
authors.initials: D
authors.lastname: Zhao
authors.forename: Hua
authors.initials: H
authors.lastname: Xiang
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000138:chemical synthesis* / Q000494:pharmacology; D019623:Haloferax mediterranei / Q000378:metabolism; D006490:Hemostatics / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D010421:Pentanoic Acids / Q000138:chemical synthesis* / Q000494:pharmacology; D010973:Platelet Adhesiveness / Q000187:drug effects / Q000502:physiology; D000091402:Prohibitins
chemical_list: D006490:Hemostatics; C000717328:PHB protein, human; D010421:Pentanoic Acids; D000091402:Prohibitins; C013056:beta-hydroxyvaleric acid; D020155:3-Hydroxybutyric Acid