title: Selective antegrade cerebral perfusion reduces brain injury following deep hypothermic circulatory arrest in the piglets' model by decreasing the levels of protein SUMO2/3-ylation.
abstract: Selective antegrade cerebral perfusion (SACP), which was adopted by many surgical groups for complex neonatal cardiac surgery, especially aortic arch repair, is a proven adjunct for neuroprotection during deep hypothermic circulatory arrest (DHCA). Several recent studies suggest that SUMO2/3 modification of proteins is markedly activated during deep hypothermia and believed to be an endogenous neuroprotective stress response. Here, we report that SACP reduces the increasing degree of SUMO2/3 conjugation following DHCA. Piglets were subjected to 1 h SACP and/or 1 h DHCA. DHCA was sufficient to markedly increase in protein SUMOylation by SUMO2/3 both in the hippocampus and cerebral cortex. SACP, especially at flow rate of 50 ml/kg/min, reduces the increasing degree of SUMO2/3 conjugation and also reduces levels of pro-apoptotic factors, Bax and Caspase 3, and increases levels of antiapoptotic factors, Bcl-2, following DHCA both in the hippocampus and cerebral cortex. This suggests that SACP at flow rate of 50 ml/kg/min is more appropriate for neuroprotection during DHCA in the pig model and level of protein SUMO2/3-ylation maybe an indicator of the degree of brain injury.
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Yaobin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Aijun
authors.initials: A
authors.lastname: Liu
authors.affiliation: Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China ; Beijing Aortic Disease Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Junwu
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University Beijing, China ; Beijing Institute of Heart, Lung and Blood Vessel Diseases Beijing, China.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Liu
keywords: SUMO2/3; Selective antegrade cerebral perfusion; apoptosis; brain injury; deep hypothermic circulatory arrest; piglet