title: Intrinsic stability of Brassicaceae plasma membrane in relation to changes in proteins and lipids as a response to salinity.
abstract: Changes in plasma membrane lipids, such as sterols and fatty acids, have been observed as a result of salt stress. These alterations, together with modification of the plasma membrane protein profile, confer changes in the physical properties of the membrane to be taken into account for biotechnological uses. In our experiments, the relationship between lipids and proteins in three different Brassicaceae species differing in salinity tolerance (Brassica oleracea, B. napus and Cakile maritima) and the final plasma membrane stability were studied. The observed changes in the sterol (mainly an increase in sitosterol) and fatty acid composition (increase in RUFA) in each species led to physical adaptation of the plasma membrane to salt stress. The in vitro vesicles stability was higher in the less tolerant (B. oleracea) plants together with low lipoxygenase activity. These results indicate that the proteins/lipids ratio and lipid composition is an important aspect to take into account for the use of natural vesicles in plant biotechnology.
authors.affiliation: Laboratory of Extremophile Plants, Center of Biotechnology of Borj-Cedria (LEP-CBBC), PO Box 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Najla
authors.initials: N
authors.lastname: Chalbi
authors.affiliation: Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Edificio 25, 30100, Murcia, Spain.
authors.forename: Ma Carmen
authors.initials: MC
authors.lastname: Martínez-Ballesta
authors.affiliation: Laboratory of Extremophile Plants, Center of Biotechnology of Borj-Cedria (LEP-CBBC), PO Box 901, 2050, Hammam-Lif, Tunisia.
authors.forename: Nabil Ben
authors.initials: NB
authors.lastname: Youssef
authors.affiliation: Departamento de Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC), Campus Universitario de Espinardo, Edificio 25, 30100, Murcia, Spain. Electronic address: mcarvaja@cebas.csic.es.
authors.forename: Micaela
authors.initials: M
authors.lastname: Carvajal
mesh_terms: D000222:Adaptation, Physiological; D019607:Brassicaceae / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000235:genetics / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance; D012965:Sodium Chloride / Q000494:pharmacology*; D013261:Sterols / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D010940:Plant Proteins; D013261:Sterols; D012965:Sodium Chloride; D008084:Lipoxygenase
keywords: Brassicaceae; Fatty acids; Lipoxygenase; Plasma membrane stability; Salinity; Sterols