title: Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene.
abstract: Insulin-like androgenic gland hormone-binding protein (IAGBP) has been investigated in crustaceans in vitro. However, the relationship between IAGBP and its putative binding protein partner insulin-like androgenic gland hormone (IAG) has not been studied at the transcriptional level in vivo. In the current study, we cloned the full-length cDNA of IAGBP from the oriental river prawn Macrobrachium nipponense (Mn-IAGBP) and investigated the transcriptional patterns of Mn-IAGBP and the M. nipponense IAG gene (Mn-IAG) at different developmental stages and in different tissues. Mn-IAGBP mRNA was detected in all examined tissues from adult male prawns, with the highest transcriptional levels in the testis. Mn-IAG mRNA was detected in the androgenic gland and hepatopancreas. The genomic sequences of Mn-IAGBP and Mn-IAG were isolated by genome walking and two gene copies were found in both Mn-IAGBP and Mn-IAG. The relationship between Mn-IAGBP and Mn-IAG at the transcriptional level was studied by RNA interference. Injection of Mn-IAGBP double-stranded RNA (dsRNA) significantly reduced the transcription of Mn-IAG, while injection of Mn-IAG dsRNA significantly reduced the transcription of Mn-IAGBP in testis, muscle, androgenic gland, and hepatopancreas. These results demonstrate the involvement of the IAGBP gene in IAG signaling in M. nipponense.
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China; Weifang University of Science and Technology, Shouguang 262700, PR China.
authors.forename: Fajun
authors.initials: F
authors.lastname: Li
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China.
authors.forename: Hongkun
authors.initials: H
authors.lastname: Bai
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China. Electronic address: fuht@ffrc.cn.
authors.forename: Hongtuo
authors.initials: H
authors.lastname: Fu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Sufei
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, PR China.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Shubo
authors.initials: S
authors.lastname: Jin
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Shengming
authors.initials: S
authors.lastname: Sun
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000728:Androgens / Q000378:metabolism; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D042341:Gonadal Hormones / Q000235:genetics / Q000378:metabolism*; D043143:Hepatopancreas / Q000378:metabolism; D007328:Insulin / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D033541:Palaemonidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000728:Androgens; D060829:Arthropod Proteins; D002352:Carrier Proteins; D018076:DNA, Complementary; D042341:Gonadal Hormones; D007328:Insulin; D012330:RNA, Double-Stranded
keywords: Insulin-like androgenic gland hormone gene; Insulin-like androgenic gland hormone-binding protein gene; Macrobrachium nipponense; RNA interference