title: Prevalence, quantification, and typing of human adenoviruses detected in river water in Taiwan.
abstract: The prevalence of human adenoviruses (HAdV) in river waters was investigated in this study. Water samples were collected from 13 rivers in Taiwan, concentrated, and assessed for the presence of HAdVs using nested polymerase chain reaction (PCR). Human AdV positive samples were then subjected to real-time PCR (qPCR) to quantify the viral genomes and further subjected to primer-based genotyping to identify the various serotypes present. For each water sample, several water quality parameters were evaluated, including heterotrophic plate count, total coliform, Escherichia coli, water temperature, pH, conductivity, and dissolved oxygen. Among the 13 rivers examined, four rivers (30.8 %) were found to contain HAdVs. The major genotype was F species HAdV serotype 41. The mean HAdVs concentrations ranged from 6.10 × 10(2) to 8.51 × 10(2) copies/L. No significant differences were observed between the presence of HAdVs, and all of the water quality parameters evaluated (heterotrophic plate count, total coliform, E. coli, water temperature, pH, conductivity, and dissolved oxygen). Given the potential health risks posed by the presence of enteric viruses in environmental waters, further assessment is desirable with respect to possible sources, virus transport, and survival of viruses in the aquatic environment.
authors.affiliation: Division of Urology, Department of Surgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan, Republic of China.
authors.forename: Zhon-Min
authors.initials: ZM
authors.lastname: Huang
authors.forename: Bing-Mu
authors.initials: BM
authors.lastname: Hsu
authors.forename: Po-Min
authors.initials: PM
authors.lastname: Kao
authors.forename: Tien-Yu
authors.initials: TY
authors.lastname: Chang
authors.forename: Tsui-Kang
authors.initials: TK
authors.lastname: Hsu
authors.forename: Ying-Ning
authors.initials: YN
authors.lastname: Ho
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Yang
authors.forename: Yu-Li
authors.initials: YL
authors.lastname: Huang
mesh_terms: D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004784:Environmental Monitoring; D004926:Escherichia coli / Q000302:isolation & purification; D005838:Genotype; D016133:Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D045483:Rivers / Q000382:microbiology / Q000821:virology*; D013624:Taiwan; D014871:Water Microbiology; D014873:Water Pollutants / Q000145:classification / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral; D014873:Water Pollutants