title: The effect of reactive oxygen species on motility parameters, DNA integrity, tyrosine phosphorylation and phosphatase activity of common carp (Cyprinus carpio L.) spermatozoa.
abstract: The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) either in the presence or absence of antioxidants for 15 and 60 min. A dose- and time-dependent reduction in spermatozoa motility and velocity was observed. Comet assays showed a dramatic increase in DNA fragmentation after 15 min. Changes in tyrosine phosphorylation of spermatozoa proteins were observed by Western blotting with anti-phosphotyrosine antibodies, and proteins of interest were identified by mass spectrometry. After a 60 min exposure to X-XO, O-linked N-acetylglucosamine transferase, isoform 4 was phosphorylated and septin-8-A was dephosphorylated. Acid phosphatase activity also decreased in a dose-dependent manner after a 60 min exposure to oxidative stress. The results demonstrate that oxidative stress impaired functional variables (sperm motility, velocity, DNA integrity) of carp spermatozoa, and altered intracellular signalling pathways through changes in tyrosine phosphorylation and acid phosphatase activity.
authors.affiliation: Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Vodňany, Czech Republic.
authors.forename: Ievgeniia
authors.initials: I
authors.lastname: Gazo
authors.forename: Anna
authors.initials: A
authors.lastname: Shaliutina-Kolešová
authors.forename: Mariola A
authors.initials: MA
authors.lastname: Dietrich
authors.forename: Pavla
authors.initials: P
authors.lastname: Linhartová
authors.forename: Olena
authors.initials: O
authors.lastname: Shaliutina
authors.forename: Jacky
authors.initials: J
authors.lastname: Cosson
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D002347:Carps / Q000378:metabolism*; D053938:DNA Fragmentation; D029941:Fish Proteins / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D017382:Reactive Oxygen Species; D000135:Acid Phosphatase