title: Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus.
abstract: Lactobacilli are widespread in natural environments and are increasingly being investigated as potential health modulators. In this study, we have adapted the broad-host-range vector pNZ8048 to express the mCherry protein (pRCR) to expand the usage of the mCherry protein for analysis of gene expression in Lactobacillus. This vector is also able to replicate in Streptococcus pneumoniae and Escherichia coli. The usage of pRCR as a promoter probe was validated in Lactobacillus acidophilus by characterizing the regulation of lactacin B expression. The results show that the regulation is exerted at the transcriptional level, with lbaB gene expression being specifically induced by co-culture of the L. acidophilus bacteriocin producer and the S. thermophilus STY-31 inducer bacterium.
authors.affiliation: Departamento de Microbiología Molecular y Biología de las Infecciones, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040, Madrid, Spain.
authors.forename: M Luz
authors.initials: ML
authors.lastname: Mohedano
authors.forename: Tomás
authors.initials: T
authors.lastname: García-Cayuela
authors.forename: Adrián
authors.initials: A
authors.lastname: Pérez-Ramos
authors.forename: Rogier A
authors.initials: RA
authors.lastname: Gaiser
authors.forename: Teresa
authors.initials: T
authors.lastname: Requena
authors.forename: Paloma
authors.initials: P
authors.lastname: López
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001430:Bacteriocins / Q000235:genetics / Q000378:metabolism; D018920:Coculture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D007779:Lactobacillus acidophilus / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; C035728:lactocin