title: TP53 codon 72 single nucleotide polymorphism in chronic lymphocytic leukemia.
abstract: Defects in the tumor suppressor gene TP53 are known to be important in chronic lymphocytic leukemia (CLL) and TP53 inactivation is associated with a particularly aggressive form of the disease. The single nucleotide polymorphism in the TP53 gene at codon 72 (rs1042522), results in amino acid substitution influencing apoptotic potential of TP53 protein. The aim of the study was to evaluate the association of the TP53 codon 72 polymorphism and incidence of TP53 mutations in CLL patients.
  
  
  METHODS
  261 CLL samples were analyzed by polymerase chain reaction and direct sequencing for TP53 mutations and single nucleotide polymorphism.
  
  
  RESULTS
  The 72Pro/Pro genotype was associated with an increased incidence of TP53 mutations in previously treated patients (OR = 2.503; 95% CI 1.142-5.487; р = 0.001).
  
  
  CONCLUSIONS
  This study revealed that the TP53 codon 72 polymorphism may be used as a risk factor for incidence of TP53 mutations in CLL.
authors.affiliation: SE "National Research Center for Radiation Medicine, National Academy of Medical Sciences of Ukraine", 53 Melnikov Str., Kyiv 04050, Ukraine.
authors.forename: N I
authors.initials: NI
authors.lastname: Bilous
authors.affiliation: SE "National Research Center for Radiation Medicine, National Academy of Medical Sciences of Ukraine", 53 Melnikov Str., Kyiv 04050, Ukraine.
authors.forename: I V
authors.initials: IV
authors.lastname: Abramenko
authors.affiliation: SE "National Research Center for Radiation Medicine, National Academy of Medical Sciences of Ukraine", 53 Melnikov Str., Kyiv 04050, Ukraine.
authors.forename: A A
authors.initials: AA
authors.lastname: Chumak
authors.affiliation: SE "National Research Center for Radiation Medicine, National Academy of Medical Sciences of Ukraine", 53 Melnikov Str., Kyiv 04050, Ukraine.
authors.forename: I S
authors.initials: IS
authors.lastname: Dyagil
authors.affiliation: SE "National Research Center for Radiation Medicine, National Academy of Medical Sciences of Ukraine", 53 Melnikov Str., Kyiv 04050, Ukraine.
authors.forename: Z V
authors.initials: ZV
authors.lastname: Martіna
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D019943:Amino Acid Substitution; D003062:Codon; D004252:DNA Mutational Analysis; D005260:Female; D005787:Gene Frequency; D016158:Genes, p53; D005838:Genotype; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D003062:Codon