title: The induction of lipid peroxidation during the acute oxidative stress response induced by intratracheal instillation of fine crystalline silica particles in rats.
abstract: Crystalline silica (SiO2) is an important material for industry but is considered potentially carcinogenic. Inhalation of a crystalline SiO2aerosol may contribute to serious lung diseases. Crystalline SiO2particles are commonly used as a positive control in toxicity assays of particulate materials (e.g. nanoparticles). Crystalline SiO2induces oxidative stress resulting in lipid peroxidation, but the acute oxidative stress response in the lung is not well understood. Lipid peroxidation during the acute stage of oxidative stress after instillation of crystalline SiO2into rats was examined by bronchoalveolar lavage fluid (BALF) analysis. The levels of 8-iso-prostaglandin F2αand hydroxyoctadecadienoic acid (HODE) in the BALF were measured using liquid chromatography coupled to quadrupole mass spectrometry. The concentration of the antioxidant protein heme oxygenase-1 (HO-1) in the BALF was determined using enzyme-linked immunosorbent assay. Intratracheal instillation of crystalline SiO2increased the level of HODE and HO-1 in BALF at 24 h after administration. The levels of HODE and HO-1 returned to baseline at 72 h after instillation. Lactate dehydrogenase leakage was observed only after 1 h instillation. These results suggest that the contribution of oxidative stress to the pulmonary toxicity of crystalline SiO2is minimal in the early acute stage after exposure.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Gifu, Japan hiroko-fukui@aist.go.jp.|Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Fukui
authors.affiliation: Research Institute for Environmental Management Technology (EMTECH), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
authors.forename: Shigehisa
authors.initials: S
authors.lastname: Endoh
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.
authors.forename: Mototada
authors.initials: M
authors.lastname: Shichiri
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ishida
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Hagihara
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Yoshida
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Iwahashi
authors.affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Horie
mesh_terms: D000393:Air Pollutants / Q000633:toxicity; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002274:Carcinogens, Environmental / Q000633:toxicity; D015237:Dinoprost / Q000819:agonists / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D005231:Fatty Acids, Unsaturated / Q000819:agonists / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000378:metabolism; D007322:Instillation, Drug; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D017208:Rats, Wistar; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000097:blood / Q000201:enzymology / Q000378:metabolism*; D014132:Trachea
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D002274:Carcinogens, Environmental; D005231:Fatty Acids, Unsaturated; D052638:Particulate Matter; C495366:hydroxyoctadecadienoic acid; C075750:8-epi-prostaglandin F2alpha; D012822:Silicon Dioxide; D015237:Dinoprost; D051547:Heme Oxygenase-1
keywords: Crystalline silica; acute phase; fine particle; intratracheal instillation; lipid peroxidation; oxidative stress