title: Inhibitory effect of ibuprofen on tumor survival and angiogenesis in gastric cancer cell.
abstract: Numerous epidemiological studies have suggested effectiveness of long-term and regular use of non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen and aspirin, in preventing and treatment of certain cancers including prostate, colon, breast, lung, and gastric cancers. We have studied the potential anti-turmeric effect of ibuprofen in adenocarcinoma gastric cell line (AGS). The effects of ibuprofen were investigated on cell proliferation, apoptosis, angiogenesis, and expression of stemness marker genes using real-time RT-PCR, DNA laddering, and tube formation assays via ECM gel and human umbilical vein endothelial cells (HUVECs). Annexin-V-FLUOS and propidium iodide (PI) were used to stain the apoptotic cells. Our findings indicate that ibuprofen at the concentrations of 100, 200, 300, 400, and 500 μM is able to reduce the cancerous characteristics of the AGS cells by inducing apoptosis, inhibition of cell proliferation, and angiogenesis. Real-time RT-PCR showed that ibuprofen altered the expression of several genes including Akt, P53, PCNA, Bax, and Bcl2 in the AGS cells. In addition, reduction in CD44 and OCT3/4 transcript levels revealed that ibuprofen reduces the stemness of the AGS cells and therefore it could be used as a potential anti-tumor drug.
authors.affiliation: Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran, hassan_akrami@yahoo.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Akrami
authors.forename: Saman
authors.initials: S
authors.lastname: Aminzadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Fallahi
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007052:Ibuprofen / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D013274:Stomach Neoplasms / Q000188:drug therapy*; D059467:Transcriptome / Q000187:drug effects
chemical_list: D020533:Angiogenesis Inhibitors; D007052:Ibuprofen