title: Effect of Mechanism of Action of Different ω-6/ω-3 Polyunsaturated Fatty Acids Ratio on the Growth of Endometrial Carcinoma Mice.
abstract: To explore the effect and mechanism of action of different ω-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio on the expression of AKT and mTOR in mice bearing endometrial carcinoma. Once the human endometrial carcinoma xenograft models were successfully established, 40 BALB/C mice were randomized into five groups: group A (ω-6 PUFAs), group B (10:1 ω-6/ω-3 PUFAs), group C (control group), group D (1:1 ω-6/ω-3 PUFAs), and group E (ω-3 PUFAs). Six weeks post-treatment, mice were sacrificed and the xenograft tissues were harvested for immunohistochemical SP analysis of AKT and mTOR expression. AKT and mTOR mRNA expression was determined by reverse transcription polymerase chain reaction. Group A and group B had the highest positive expression of AKT and mTOR, with increased mRNA expression. Group D and group E had the lowest positive expression of AKT and mTOR, with decreased mRNA expression. There was a positive correlation between the expression of AKT and that of mTOR (r = 0.92). Thus, ω-6/ω-3 PUFAs in different proportions are associated with the mRNA expression of AKT and mTOR in the tissues of mouse xenograft model of human endometrial cancer.
authors.affiliation: Department of Gynaecology and Obstetrics, Affiliated Hospital of Xuzhou Medical College, No.99 Huaihai West Road, Xuzhou, 221000, Jiangsu, China. contribution037@163.com.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Gynaecology and Obstetrics, Affiliated Hospital of Xuzhou Medical College, No.99 Huaihai West Road, Xuzhou, 221000, Jiangsu, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Gynaecology and Obstetrics, Affiliated Hospital of Xuzhou Medical College, No.99 Huaihai West Road, Xuzhou, 221000, Jiangsu, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jing
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016889:Endometrial Neoplasms / Q000473:pathology*; D015525:Fatty Acids, Omega-3 / Q000502:physiology*; D043371:Fatty Acids, Omega-6 / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases
keywords: AKT; Endometrial carcinoma; Polyunsaturated fatty acids; mTOR