title: Adsorption-synergic biodegradation of diesel oil in synthetic seawater by acclimated strains immobilized on multifunctional materials.
abstract: Using enrichment culture technique, three isolates marked as ODB-1, ODB-2 and ODB-3, were selected from oil contaminated seawater. 16S rDNA gene sequencing indicated that ODB-1 affiliated with Pseudomonas sp. while ODB-2 and ODB-3 affiliated with Brevundimonas sp. Subsequently, the bacterial cells were immobilized on the surface of expanded graphite (EG), expanded perlite (EP) and bamboo charcoal (BC). Among the three isolates, ODB-1 showed a strong binding to the bio-carriers through extracellular polysaccharides, while ODB-2 and ODB-3 made the adhesion to bio-carrier through direct physical adsorption. The immobilized bacteria exhibited good salinity tolerance compared with the planktonic bacteria. Their total diesel oil removal rates were more than 85% after 6 days' incubation. Adsorption-biodegradation process played an important role in the oil-pollution remediation. EG-bacteria system was treated as a promising remediation method, which achieved nearly 100% removal of diesel oil. Thereinto, over 83% removal of diesel oil owed to biodegradation.
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China. Electronic address: wangxj@tongji.edu.cn.
authors.forename: Xuejiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Mian
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Yunjie
authors.initials: Y
authors.lastname: Bu
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Shanghai 200092, China.
authors.forename: Jianfu
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000064:Acclimatization; D000327:Adsorption; D000537:Aluminum Oxide; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D018914:Cells, Immobilized / Q000378:metabolism; D002606:Charcoal; D004275:DNA, Ribosomal; D005742:Gasoline; D006108:Graphite; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D012822:Silicon Dioxide; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004275:DNA, Ribosomal; D005742:Gasoline; D014874:Water Pollutants, Chemical; C003076:Perlite; D002606:Charcoal; D012822:Silicon Dioxide; D006108:Graphite; D000537:Aluminum Oxide
keywords: Adsorption; Bio-carrier; Biodegradation; Diesel oil; Immobilization