title: Micronuclei and other nuclear anomalies in exfoliated buccal mucosa cells of Mexican women with breast cancer.
abstract: OBJECTIVE
  Breast cancer (BC) is the most frequently diagnosed form of cancer and the leading cause of cancer-related deaths among females in the world. RESULTS of several studies showed that the genome of primary cancer patients (naive for any treatment) is unstable. The purpose of the present study was to evaluate the genomic instability in BC patients by means of buccal cells micronucleus (MN) cytome assay Methods: The frequencies of nuclear anomalies including MN, binucleates (BN), broken eggs (BE), condensed chromatin (CC), karyorrhexis (KR) and karyolysis (KL) were evaluated in exfoliated buccal mucosa cells of Mexican women with primary BC and healthy women. Buccal cells were collected from 21 BC patients (9 with stage I and 12 with stage II) and from 20 healthy females used as control group.
  
  
  RESULTS
  The results of the evaluation of cells showed that the frequencies of MN, BN, BE, KR and KL were significantly increased in the pooled group of BC patients compared with the control group. However, no one parameter of buccal MN-cytome assay in patients with stage I BC was significant compared with controls and BC patients with stage II.
  
  
  CONCLUSIONS
  Application of the buccal MN-cytome assay for the study of genomic instability in primary BC patients showed that both genotoxic and cytotoxic effects can be evaluated in such patients.
authors.affiliation: Unidad Académica de Medicina, Universidad Autónoma de Nayarit; Tepic, Nayarit, México;
authors.forename: Aurelio
authors.initials: A
authors.lastname: Flores-Garcia
authors.forename: Olivia
authors.initials: O
authors.lastname: Torres-Bugarin
authors.forename: Jesús
authors.initials: J
authors.lastname: Salvador Velarde-Félix
authors.forename: Héctor
authors.initials: H
authors.lastname: Rangel-Villalobos
authors.forename: Eloy Alfonso
authors.initials: EA
authors.lastname: Zepeda-Carrillo
authors.forename: Amelia
authors.initials: A
authors.lastname: Rodríguez-Trejo
authors.forename: Pedro
authors.initials: P
authors.lastname: Aguíar-García
authors.forename: Armen
authors.initials: A
authors.lastname: Nersesyan
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D002467:Cell Nucleus; D005260:Female; D006801:Humans; D015162:Micronucleus Tests; D009061:Mouth Mucosa / Q000166:cytology