title: Encapsulation of carvacrol, a monoterpene present in the essential oil of oregano, with β-cyclodextrin, improves the pharmacological response on cancer pain experimental protocols.
abstract: Cancer pain is a major public health problem worldwide due to the strong impact on the quality of life of patients and side effects of the existing therapeutic options. Monoterpenes, as carvacrol (CARV), have been extensively studied about their therapeutic properties, especially their importance in the control of painful conditions and inflammation, which can be improved through the use of inclusion complexes of β-cyclodextrin (β-CD). We evaluated the effect of encapsulation of CARV in β-CD (CARV/β-CD) on the nociception induced by tumor cells (Sarcoma 180) in rodents. Inclusion complexes were prepared in two different procedures and characterized through thermal analysis and scanning electron microscopy. CARV/β-CD complex was administered (50 mg/kg, p.o.) in mice with tumor on the hind paw and was able to reduce the hyperalgesia (von Frey) during 24 h, unlike the free CARV (100 mg/kg, p.o.), which promoted effects until 9 h. Administration on alternate days of complex of CARV/β-CD (12.5-50 mg/kg, p.o.) reduced hyperalgesia, as well as spontaneous and palpation-induced nociception. However, pure CARV (50 mg/kg) did not cause significant changes in nociceptive responses. Together, these results produced evidence that the encapsulation of carvacrol in β-cyclodextrin can be useful for the development of new options for pain management.
authors.affiliation: Department of Health Education, Federal University of Sergipe, Lagarto, SE, Brazil.
authors.forename: Adriana Gibara
authors.initials: AG
authors.lastname: Guimarães
authors.affiliation: Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
authors.forename: Marlange Almeida
authors.initials: MA
authors.lastname: Oliveira
authors.affiliation: Department of Physiology, Federal University of Sergipe, São Cristóvão, SE, Brazil.
authors.forename: Rafael dos Santos
authors.initials: Rdos S
authors.lastname: Alves
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Lagarto, São Cristóvão, SE, Brazil.
authors.forename: Paula dos Passos
authors.initials: Pdos P
authors.lastname: Menezes
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Lagarto, São Cristóvão, SE, Brazil.
authors.forename: Mairim Russo
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Lagarto, São Cristóvão, SE, Brazil.
authors.forename: Adriano Antunes de Souza
authors.initials: AA
authors.lastname: Araújo
authors.affiliation: Oswaldo Cruz Foundation, Laboratory of Tissue Engineering and Immunopharmacology, Salvador, BA, Brazil.
authors.forename: Daniel Pereira
authors.initials: DP
authors.lastname: Bezerra
authors.affiliation: Department of Health Education, Federal University of Sergipe, Lagarto, SE, Brazil. Electronic address: lucindojr@gmail.com.
authors.forename: Lucindo José
authors.initials: LJ
authors.lastname: Quintans Júnior
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical; D000081007:Cymenes; D006930:Hyperalgesia / Q000188:drug therapy; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D059225:Nociception / Q000187:drug effects; D009822:Oils, Volatile / Q000737:chemistry*; D031341:Origanum / Q000737:chemistry* / Q000378:metabolism; D010146:Pain / Q000517:prevention & control*; D010173:Palpation; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D047392:beta-Cyclodextrins; C073316:carvacrol; C031215:betadex
keywords: Cancer pain; Carvacrol; Hyperalgesia; Monoterpene; Nociception; β-Cyclodextrin