title: Glutathione-coordinated [2Fe-2S] cluster: a viable physiological substrate for mitochondrial ABCB7 transport.
abstract: The glutathione-coordinated [2Fe-2S] cluster is demonstrated to be a viable and likely substrate for physiological iron-sulfur cluster transport by Atm1p, a mitochondrial ABC export protein. Flow cytometry and colorimetric assays demonstrate a quantitative methodology for study of metal translocation proteins and their proteoliposome products.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 100 West 18th Ave, Columbus, OH 43210, USA. cowan@chemistry.ohio-state.edu.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Li
authors.forename: J A
authors.initials: JA
authors.lastname: Cowan
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000378:metabolism*; D056831:Coordination Complexes / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism*; D011510:Proteolipids / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018528:ATP-Binding Cassette Transporters; D056831:Coordination Complexes; D011510:Proteolipids; C020485:proteoliposomes; D013455:Sulfur; D007501:Iron; D005978:Glutathione