title: Effect of rCaIFN-γ pretreatment on propofol-isoflurane suppression of NK cytotoxic activity in the peripheral blood of dogs.
abstract: In dogs, natural killer (NK) cell cytotoxic activity, which is an index of antitumor immunity, decreases after general anesthesia. In this study, we examined whether the decrease in NK cytotoxic activity can be controlled with interferon-gamma (IFN-γ) treatment. Beagles were divided into 2 groups: a treated group (n = 6) that received recombinant canine IFN-γ (rCaIFN-γ) and an untreated control group (n = 6). Blood samples were taken before and at 24, 120, and 192 hours after anesthesia. NK cytotoxic activity toward canine thyroid cancer cells was measured in isolated lymphocytes with the Rose Bengal assay. The decrease in NK cytotoxic activity after anesthesia was significantly inhibited by administration of rCaIFN-γ before propofol-isoflurane anesthesia. Further studies are necessary to evaluate the clinical implications of rCaIFN-γ treatment in tumor recurrence and morbidity.
authors.affiliation: Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan. Electronic address: sur.miyata@gmail.com.
authors.forename: Takuma
authors.initials: T
authors.lastname: Miyata
authors.affiliation: Division of Therapeutic Sciences II, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Honma
authors.affiliation: Division of Therapeutic Sciences II, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Asaka
authors.initials: A
authors.lastname: Sato
authors.affiliation: Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Division of Therapeutic Sciences I, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Koyama
authors.affiliation: Division of Therapeutic Sciences II, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Tagawa
mesh_terms: D056704:Adaptive Immunity / Q000502:physiology; D000768:Anesthesia, General; D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000662:veterinary*; D004285:Dogs / Q000276:immunology / Q000502:physiology*; D005260:Female; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000008:administration & dosage / Q000494:pharmacology*; D007530:Isoflurane / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000502:physiology; D008297:Male; D009369:Neoplasms / Q000276:immunology / Q000662:veterinary; D011184:Postoperative Period; D057234:Preoperative Period; D015742:Propofol / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000777:Anesthetics; D011994:Recombinant Proteins; D007371:Interferon-gamma; D007530:Isoflurane; D015742:Propofol
keywords: Dog; IFN-γ; Isoflurane; NK cytotoxic activity