title: [Expression of chemokine receptor CXCR7 in gastric cancer tissues and cell lines].
abstract: OBJECTIVE
  To investigate the expression status of CXCR7 in gastric cancer tissues and cell lines.
  
  
  METHODS
  The expression status of CXCR7 was detected in 35 primary gastric cancer tissues and matched adjacent tissues by immunohistochemistry and RT-PCR. The correlation of CXCR7 expression with the clinicopathological parameters and risk factors of gastric cancer was analyzed. The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, BGC-823, SGC-7901 and MKN-28) was also detected by immunofluorescence assay.
  
  
  RESULTS
  The expression of CXCR7 was significantly higher in gastric cancer tissues than in adjacent tissues (P<0.01). CXCR7 expression was not correlated with age, gender, smoking history, Helicobacter pylori infection, tumor location or the pathological type, but showed a higher expression level in patients with a alcohol-drinking history than in those without (P<0.05). CXCR7 was expressed with variable intensities in the 5 gastric cancer cell lines without correlation with the degrees of cell differentiation; its expression was the highest in SGC-7901 cells, a moderately differentiated human gastric adenocarcinoma cell line.
  
  
  CONCLUSIONS
  CXCR7 is highly expressed in gastric cancer tissues with variable intensities in 5 gastric cancer cell lines, suggesting its important role in gastric cancer progression.
authors.affiliation: Department of Gastroenterology, Second Affiliate Hospital of Medical College, Xian Jiao Tong University, Xi'an 710004, China.E-mail: sam19880913@126.com.
authors.forename: Ameng
authors.initials: A
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
authors.forename: Miao
authors.initials: M
authors.lastname: Jia
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guo
authors.forename: Jiong
authors.initials: J
authors.lastname: Jiang
authors.forename: Bin
authors.initials: B
authors.lastname: Qin
mesh_terms: D002454:Cell Differentiation; D045744:Cell Line, Tumor; D018450:Disease Progression; D016481:Helicobacter Infections; D006801:Humans; D007150:Immunohistochemistry; D054387:Receptors, CXCR / Q000378:metabolism*; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000378:metabolism*
chemical_list: C501896:ACKR3 protein, human; D054387:Receptors, CXCR