title: MicroRNA-503 acts as a tumor suppressor in osteosarcoma by targeting L1CAM.
abstract: Deregulated microRNAs and their roles in tumorigenesis have attracted much attention in recent years. Although miR-503 was shown to be important in tumorigenesis, its role in osteosarcoma remains unknown. In this study, we focused on the expression and mechanisms of miR-503 in osteosarcoma development. We found that miR-503 was down-regulated in osteosarcoma cell lines and primary tumor samples, and the restoration of miR-503 reduced cell proliferation, migration and invasion. Low level of miR-503 in patients with osteosarcoma was associated with considerably shortened disease-free survival. Furthermore, bioinformatic prediction and experimental validation revealed that the anti-tumor effect of miR-503 was probably exerted through targeting and repressing of L1CAM expression. L1CAM was up-regulated in osteosarcoma cell lines and primary tumor samples and the expression level of L1CAM were negatively correlated with miR-503 levels in osteosarcoma tissues. Collectively, our data identify the important roles of miR-503 in osteosarcoma pathogenesis, indicating its potential application in cancer therapy.
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chong
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Xinzhen
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Shiqian
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Department of Orthopedic Surgery, the First Affiliated Hospital of Harbin Medical University, No. 23, Youzheng St., Nangang District, Harbin, Heilongjiang Prov.150001, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Shao
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002648:Child; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology
chemical_list: C545473:MIRN503 microRNA, human; D035683:MicroRNAs; D039842:Neural Cell Adhesion Molecule L1