title: Effect of smoke derivatives on in vitro pollen germination and pollen tube elongation of species from different plant families.
abstract: Plant-derived smoke stimulates seed germination in numerous plant species. Smoke also has a positive stimulatory effect on pollen germination and pollen tube growth. The range of plant families affected my smoke still needs to be established since the initial study was restricted to only three species from the Amaryllidaceae. The effects of smoke-water (SW) and the smoke-derived compounds, karrikinolide (KAR1 ) and trimethylbutenolide (TMB) on pollen growth characteristics were evaluated in seven different plant families. Smoke-water (1:1000 and 1:2000 v:v) combined with either Brewbaker and Kwack's (BWK) medium or sucrose and boric acid (SB) medium significantly improved pollen germination and pollen tube growth in Aloe maculata All., Kniphofia uvaria Oken, Lachenalia aloides (L.f.) Engl. var. aloides and Tulbaghia simmleri P. Beauv. Karrikinolide (10(-6) and 10(-7) m) treatment significantly improved pollen tube growth in A. maculata, K. uvaria, L. aloides and Nematanthus crassifolius (Schott) Wiehle compared to the controls. BWK or SB medium containing TMB (10(-3) m) produced significantly longer pollen tubes in A. maculata, K. uvaria and N. crassifolius. These results indicate that plant-derived smoke and the smoke-isolated compounds may stimulate pollen growth in a wide range of plant species.
authors.affiliation: Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.
authors.forename: A
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.
authors.forename: H B
authors.initials: HB
authors.lastname: Papenfus
authors.affiliation: Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.
authors.forename: M G
authors.initials: MG
authors.lastname: Kulkarni
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Pošta
authors.affiliation: Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.
authors.forename: J
authors.initials: J
authors.lastname: Van Staden
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D003470:Culture Media; D005663:Furans / Q000494:pharmacology*; D019668:Liliaceae / Q000187:drug effects*; D011058:Pollen / Q000187:drug effects* / Q000254:growth & development; D053205:Pollen Tube / Q000187:drug effects* / Q000254:growth & development; D011714:Pyrans / Q000494:pharmacology*; D012906:Smoke; D014867:Water
chemical_list: D003470:Culture Media; D005663:Furans; D011714:Pyrans; D012906:Smoke; C557869:karrikinolide; D014867:Water; C004511:butenolide; D015107:4-Butyrolactone
keywords: Karrikinolide; pollen germination; pollen tube growth; smoke-water; trimethylbutenolide