title: Quantifiler® Trio Kit and forensic samples management: a matter of degradation.
abstract: DNA collected from crime scenes may have experienced different levels of degradation. This is mainly due to sample exposure to different environmental factors. The impact of DNA degradation on short tandem repeat (STR) profiling can lead to partial or null information and in some cases, the identification of the trace may fail. The availability of a system enabling the assessment not only of the quantity of the DNA but also of its quality in terms of degradation would result in shorter time for sample processing, more reliable identifications and cost reduction by predicting the quality of the DNA profiles prior to STR analysis. We report here a study on 181 selected degraded DNA samples extracted from real crime scene evidence. The selected samples were processed by combining the use of a new commercial quantification kit (Quantifiler® Trio) with a new 24 marker multiplex PCR amplification kit (Globalfiler® Kit). Applying different statistical analyses we investigated the reliability of the Degradation Index provided by the Quantifiler® Trio in determining the level of DNA degradation in a forensic sample. This useful information can be used to predict the quality of the profile obtained after STR amplification. The combination of such a quantification kit with different PCR protocols allowed us to define practical guidelines for processing degraded forensic DNA samples with a simplified and comprehensive approach.
authors.affiliation: Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Vernarecci
authors.affiliation: Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.
authors.forename: Enrica
authors.initials: E
authors.lastname: Ottaviani
authors.affiliation: Life Sciences Solutions, Thermo Fisher Scientific, Monza, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Agostino
authors.affiliation: Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Mei
authors.affiliation: Life Sciences Solutions, Thermo Fisher Scientific, South San Francisco, CA, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Calandro
authors.affiliation: Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy. Electronic address: paola.montagna@interno.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Montagna
mesh_terms: D053836:DNA Degradation, Necrotic; D016172:DNA Fingerprinting / Q000295:instrumentation / Q000379:methods*; D053803:Forensic Genetics / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D018895:Microsatellite Repeats; D011180:Postmortem Changes; D011933:Reagent Kits, Diagnostic; D013048:Specimen Handling / Q000379:methods
chemical_list: D011933:Reagent Kits, Diagnostic
keywords: DNA degradation; Degradation Index; Globalfiler(®); Quantification; Quantifiler(®) Trio; Short tandem repeat (STR)