title: Characterization of the substitution pattern of cellulose derivatives using carbohydrate-binding modules.
abstract: BACKGROUND
  Derivatized celluloses, such as methylcellulose (MC) and hydroxypropyl methylcellulose (HPMC), are of pharmaceutical importance and extensively employed in tablet matrices. Each batch of derivatized cellulose is thoroughly characterized before utilized in tablet formulations as batch-to-batch differences can affect drug release. The substitution pattern of the derivatized cellulose polymers, i.e. the mode on which the substituent groups are dispersed along the cellulose backbone, can vary from batch-to-batch and is a factor that can influence drug release.
  
  
  RESULTS
  In the present study an analytical approach for the characterization of the substitution pattern of derivatized celluloses is presented, which is based on the use of carbohydrate-binding modules (CBMs) and affinity electrophoresis. CBM4-2 from Rhodothermus marinus xylanase 10A is capable of distinguishing between batches of derivatized cellulose with different substitution patterns. This is demonstrated by a higher migration retardation of the CBM in acrylamide gels containing batches of MC and HPMC with a more heterogeneous distribution pattern.
  
  
  CONCLUSIONS
  We conclude that CBMs have the potential to characterize the substitution pattern of cellulose derivatives and anticipate that with use of CBMs with a very selective recognition capacity it will be possible to more extensively characterize and standardize important carbohydrates used for instance in tablet formulation.
authors.forename: Laura
authors.initials: L
authors.lastname: von Schantz
authors.forename: Herje
authors.initials: H
authors.lastname: Schagerlöf
authors.forename: Eva
authors.initials: E
authors.lastname: Nordberg Karlsson
authors.forename: Mats
authors.initials: M
authors.lastname: Ohlin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry*; D015394:Molecular Structure; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism; D045245:Rhodothermus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D011956:Receptors, Cell Surface; C055213:saccharide-binding proteins; D002482:Cellulose