title: Ameliorative effect of Allolobophora caliginosa extract on hepatotoxicity induced by silicon dioxide nanoparticles.
abstract: This study aims to evaluate the possible ameliorative effect of earthworm (Allolobophora caliginosa) extract (EE) against silicon dioxide nanoparticles (SiNPs)-induced liver injury in male albino rats. The effectiveness of EE was compared with silymarin as a standard hepatoprotective drug. The present work demonstrates the antioxidant activity of EE by 1,1-diphenyl-2-picrylhydrazyl assay. Administration of SiNPs, for 15 consecutive days, caused changes in most of the biochemical parameters, namely, serum aminotransferase enzymes activities (alanine transaminase and aspartate transaminase), alkaline phosphatase activity, total protein, total and direct bilirubin level, malondialdehyde, glutathione reduced, catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase. In addition, administration of SiNPs induced changes in liver tissue architecture. Administration of EE, for subsequent 30 days, to SiNPs exposure demonstrated significant ameliorative effects on nearly all the studied parameters, and such effects were compatible with those of silymarin. In addition, the administration of EE repairs, to some extent, the abnormal architecture of the liver tissue induced by SiNPs.
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt.
authors.forename: Shimaa A
authors.initials: SA
authors.lastname: Sadek
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt soliman.amel5@gmail.com.
authors.forename: Amel M
authors.initials: AM
authors.lastname: Soliman
authors.affiliation: Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Marzouk
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D004534:Egypt; D020834:Ethnopharmacology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D009835:Oligochaeta / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D011897:Random Allocation; D051381:Rats; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry / Q000633:toxicity*; D013499:Surface Properties; D014020:Tissue Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000627:therapeutic use*; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014020:Tissue Extracts; D012822:Silicon Dioxide
keywords: Earthworm extract; liver injury; oxidative stress; silicon dioxide nanoparticles