title: Identification, expression pattern and functional characterization of As-MyD88 in bacteria challenge and during different developmental stages of Artemia sinica.
abstract: Myeloid differentiation factor 88 (MYD88), a key adapter protein in Toll-like receptor signaling, affects the immune response and the formation of the dorsal-ventral axis. Here, the 1555bp full-length cDNA of MyD88 from Artemia sinica (As-MyD88) was obtained. Molecular characterization revealed that the sequence includes an 1182bp open reading frame encoding a predicted protein of 393 amino acids. The predicted protein contains a death domain in the N-terminus, and box1 and 2 motifs of the TIR domain in the C-terminus. Real-time quantitative PCR, Western blotting and immunohistochemistry were used to determine the expression level, protein production and location of As-MYD88 during embryonic development and bacterial challenge. The highest expression level during embryonic development was at the 0h and 5h stages of A. sinica. As-MYD88 was remarkably upregulated after bacterial challenge. Our results suggested that As-MYD88 plays a vital role in response to bacterial challenge, and during post-diapause embryonic development of A. sinica.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Qin
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Huazhong
authors.initials: H
authors.lastname: Ba
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Zhenegmin
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China. Electronic address: houlin@lnnu.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, 116044, China. Electronic address: zouxiangyang@126.com.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics / Q000254:growth & development* / Q000382:microbiology*; D001419:Bacteria / Q000378:metabolism*; D001483:Base Sequence; D047108:Embryonic Development / Q000235:genetics; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D053594:Myeloid Differentiation Factor 88
keywords: Artemia sinica; Bacterial infection; Development; Gene expression pattern; MyD88