title: Inhibition of metastasis and invasion of ovarian cancer cells by crude polysaccharides from rosa roxburghii tratt in vitro.
abstract: BACKGROUND
  Rosa Roxburghii Tratt is a promising wild fruit crop in Southwest China. Its extracts have been used as traditional Chinese medicine, which benefit immune responses and cure various health disorders. However, whether Rosa Roxburghii Tratt polysaccharides could inhibit metastasis and invasion of ovarian cancer cells remains unknown.
  
  
  METHODS
  Effects of crude polysaccharides from Rosa Roxburghii Tratt on the viability of ovarian cancer A2780 cells were detected by MTT assay. Ovarian carcinoma cell migration and invasion after exposure to Rosa Roxburghii Tratt polysaccharides were quantified by wound healing and Transwell assays, respectively. Western blotting was applied to assess protein levels of MMP-9.
  
  
  RESULTS
  The results indicated that Rosa Roxburghii Tratt polysaccharides significantly reduced wound closure rate of A2780 cells, inhibited their migration and invasion, and suppressed the expression of MMP-9.
  
  
  CONCLUSIONS
  Our findings indicated that Rosa Roxburghii Tratt polysaccharides have potential for develop as anti-metastatic cancer drug preparations for ovarian cancer patients.
authors.affiliation: Department of Biotechnology, Dalian Medical University, Dalian, Liaoning, China E-mail : liweiling2006@hotmail.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhong-Jing
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Li-Kun
authors.initials: LK
authors.lastname: Liu
authors.forename: Er-Shao
authors.initials: ES
authors.lastname: Zhang
authors.forename: Wei-Ling
authors.initials: WL
authors.lastname: Li
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D011134:Polysaccharides / Q000494:pharmacology*; D027826:Rosa
chemical_list: D011134:Polysaccharides; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9