title: Low temperature inhibits root growth by reducing auxin accumulation via ARR1/12.
abstract: Plants exhibit reduced root growth when exposed to low temperature; however, how low temperature modulates root growth remains to be understood. Our study demonstrated that low temperature reduces both meristem size and cell number, repressing the division potential of meristematic cells by reducing auxin accumulation, possibly through the repressed expression of PIN1/3/7 and auxin biosynthesis-related genes, although the experiments with exogenous auxin application also suggest the involvement of other factor(s). In addition, we verified that ARABIDOPSIS RESPONSE REGULATOR 1 (ARR1) and ARR12 are involved in low temperature-mediated inhibition of root growth by showing that the roots of arr1-3 arr12-1 seedlings were less sensitive than wild-type roots to low temperature, in terms of changes in root length and meristem cell number. Furthermore, low temperature reduced the levels of PIN1/3 transcripts and the auxin level to a lesser extent in arr1-3 arr12-1 roots than in wild-type roots, suggesting that cytokinin signaling is involved in the low-temperature-mediated reduction of auxin accumulation. Taken together, our data suggest that low temperature inhibits root growth by reducing auxin accumulation via ARR1/12.
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Kun-Xiao
authors.initials: KX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Wen-Shu
authors.initials: WS
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Gong
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Liu
authors.affiliation: College of Chemistry and Biology, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China.
authors.forename: Hong-Guo
authors.initials: HG
authors.lastname: Chen
authors.affiliation: Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China yingtlu@whu.edu.cn xuhh@hhit.edu.cn.
authors.forename: Heng-Hao
authors.initials: HH
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China yingtlu@whu.edu.cn xuhh@hhit.edu.cn.
authors.forename: Ying-Tang
authors.initials: YT
authors.lastname: Lu
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002452:Cell Count; D002455:Cell Division; D003080:Cold Temperature; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D018519:Meristem / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C117138:ARR1 protein, Arabidopsis; C519632:ARR12 protein, Arabidopsis; D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D007210:Indoleacetic Acids; D014157:Transcription Factors
keywords: Auxin; Low temperature; Root meristem