title: Stabilized, superparamagnetic functionalized graphene/Fe3O4@Au nanocomposites for a magnetically-controlled solid-state electrochemiluminescence biosensing application.
abstract: Herein, a multifunctional nanoarchitecture has been developed by integrating the branched poly(ethylenimine) functionalized graphene/iron oxide hybrids (BGNs/Fe3O4) and luminol capped gold nanoparticles (luminol-AuNPs). The luminescent luminol-AuNPs as an electrochemiluminescence marker can be assembled on the nanocarrier of BGNs/Fe3O4 hybrids efficiently via the Au-N chemical bonds and electrostatic adsorption. Meanwhile, the multifunctional nanoarchitecture has been proved with excellent electron transfer, good stability, high emission intensity, etc. Furthermore, we successfully developed an ultrasensitive magnetically-controlled solid-state electrochemiluminescence (ECL) platform for label-free determination of HeLa cells using this multifunctional nanocomposite. Excellent performance of the magnetically-controlled ECL biosensing platform has been achieved including a high sensitivity for HeLa cells with a linear range from 20 to 1 × 10(4) cells/mL, good stability, and reproducibility.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , Changchun, Jilin 130022, PR China.
authors.forename: Wenling
authors.initials: W
authors.lastname: Gu
authors.forename: Xi
authors.initials: X
authors.lastname: Deng
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Gu
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Jia
authors.forename: Baohua
authors.initials: B
authors.lastname: Lou
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D005260:Female; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis
chemical_list: D049408:Luminescent Agents; D008165:Luminol; D006046:Gold; D006108:Graphite; D052203:Ferrosoferric Oxide