title: Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells.
abstract: Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.
authors.affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Manaka
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan. Electronic address: tanabe.natsuko@nihon-u.ac.jp.
authors.forename: Natsuko
authors.initials: N
authors.lastname: Tanabe
authors.affiliation: Department of Orthodontics, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Kariya
authors.affiliation: Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan; Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Masako
authors.initials: M
authors.lastname: Naito
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan; Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Takayama
authors.affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Nagao
authors.affiliation: Department of Prosthodontics, School of Dentistry, Shandong University, Jinan, Shandong Province, China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: Nihon University, Tokyo, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.affiliation: Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan; Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Maeno
authors.affiliation: Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan; Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Miyazaki
mesh_terms: D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics* / Q000528:radiation effects; D049109:Cell Proliferation / Q000528:radiation effects; D006801:Humans; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism / Q000528:radiation effects; D010012:Osteogenesis / Q000235:genetics* / Q000528:radiation effects; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism*; D013016:Sound
chemical_list: D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase
keywords: Adenosine triphosphate; Low-intensity pulsed ultrasound; Osteogenesis; P2X7