title: Increases in PKC gamma expression in trigeminal spinal nucleus is associated with orofacial thermal hyperalgesia in streptozotocin-induced diabetic mice.
abstract: Painful diabetic polyneuropathy (PDN) at the early phrase of diabetes frequently exhibits increased responsiveness to nociception. In diabetic patients and animal models, alterations in the transmission of orofacial sensory information have been demonstrated in trigeminal system. Herein, we examined the changes of protein kinase Cγ subunit (PKCγ) in trigeminal spinal nucleus (Sp5C) and observed the development of orofacial thermal sensitivity in streptozotocin (STZ)-induced type 1 diabetic mice. With hyperglycemia and body weight loss, STZ mice exhibited orofacial thermal hyperalgesia, along with increased PKCγ expression in Sp5C. Insulin treatment at the early stage of diabetes could alleviate the orofacial thermal hyperalgesia and impaired increased PKCγ in Sp5C in diabetic mice. In summary, our results demonstrate that PKCγ might be involved in orofacial thermal hyperalgesia of diabetes, and early insulin treatment might be effective way to treat orofacial PDN.
authors.affiliation: Department of Cardiology, Daping Hospital and the Research Institute of Surgery of the Third Military Medical University, 400042 Chongqing, China.
authors.forename: Hong-Ying
authors.initials: HY
authors.lastname: Xie
authors.affiliation: The Oncology Radiotherapy Center of PLA 302 Hospital, Beijing 100039, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Vascular Surgery, PLA General Hospital, Beijing, 100853, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Stomatology, General Hospital of Shenyang Military Area Command, Shenyang, China.
authors.forename: Zhao-Bin
authors.initials: ZB
authors.lastname: Zeng
authors.affiliation: Department of Cardiology, PLA General Hospital, Beijing, 100853, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhang
authors.affiliation: The Oncology Radiotherapy Center of PLA 302 Hospital, Beijing 100039, China.
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Xu
authors.affiliation: Oncology Dept.2of PLA General Hospital, Bejing 100853, China. Electronic address: qianniansong1@163.com.
authors.forename: Nian-Song
authors.initials: NS
authors.lastname: Qian
authors.affiliation: Department of Internal Medicine, Henry Ford Health System, Detroit, MI 48202, USA. Electronic address: ygz1000@gmail.com.
authors.forename: Yi-Guan
authors.initials: YG
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000201:enzymology*; D003929:Diabetic Neuropathies / Q000188:drug therapy / Q000201:enzymology*; D005145:Face; D006358:Hot Temperature; D006930:Hyperalgesia / Q000209:etiology*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D009055:Mouth; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D014278:Trigeminal Nuclei / Q000201:enzymology*
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; C087857:protein kinase C gamma; D011493:Protein Kinase C
keywords: Diabetes; Mouse; Orofacial pain; Streptozotocin; Trigeminal spinal nucleus