title: Specific visualization of tumor cells using upconversion nanophosphors.
abstract: The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the "transparency window" of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Grebenik
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Generalova
authors.affiliation: Lomonosov Moscow State University, GSP-1, Leninskie Gory, Moscow, 119991, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Nechaev
authors.affiliation: Institute of Laser and Information Technologies, Russian Academy of Sciences, Pionerskaya Str., 2, Troitsk, 142190, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Khaydukov
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
authors.forename: K E
authors.initials: KE
authors.lastname: Mironova
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Stremovskiy
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia.
authors.forename: E N
authors.initials: EN
authors.lastname: Lebedenko
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia ; Lobachevsky Nizhniy Novgorod State University, Gagarina Prospekt, 23, Nizhniy Novgorod, 603950, Russia ; Department of Physics and Astronomy, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: A V
authors.initials: AV
authors.lastname: Zvyagin
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, Moscow, 117997, Russia ; Lobachevsky Nizhniy Novgorod State University, Gagarina Prospekt, 23, Nizhniy Novgorod, 603950, Russia.
authors.forename: S M
authors.initials: SM
authors.lastname: Deyev
keywords: HER2; anti-tumor antibodies; biomarker imaging; self-assembly; upconversion nanophosphors