title: In vitro development of canine somatic cell nuclear transfer embryos in different culture media.
abstract: The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
authors.affiliation: Division of Animal Biotechnology, National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Korea.kimdhhj@korea.kr.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Jin-Gu
authors.initials: JG
authors.lastname: No
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Choi
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Yeom
authors.forename: Dong-Kyo
authors.initials: DK
authors.lastname: Kim
authors.forename: Byoung-Chul
authors.initials: BC
authors.lastname: Yang
authors.forename: Jae Gyu
authors.initials: JG
authors.lastname: Yoo
authors.forename: Min Kyu
authors.initials: MK
authors.lastname: Kim
authors.forename: Hong-Tea
authors.initials: HT
authors.lastname: Kim
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D019976:Cloning, Organism / Q000662:veterinary*; D003470:Culture Media / Q000378:metabolism; D004285:Dogs / Q000196:embryology*; D046149:Embryo Culture Techniques / Q000662:veterinary*; D047108:Embryonic Development; D053652:Nuclear Transfer Techniques / Q000662:veterinary*
chemical_list: D003470:Culture Media
keywords: G1/G2 media; canine; in vitro culture; somatic cell nuclear transfer embryo