title: [Effects of rhG-CSF Stimulation in vitro on the Adhesion and Polarization of Human CD4⁺T Lymphocytes].
abstract: The adhesion and polarization of T lymphocytes involved in the adhesive interaction of lymphocyte function-associated antigen 1 (LFA-1) with its ligand intercellular adhesion molecule 1 (ICAM-1). This study was aimed to investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) stimulation in vitro on the adhesion and polarization of CD4⁺ T cells of healthy human in peripheral blood. The peripheral blood mononuclear cells were collected from 12 healthy volunteers. The CD4⁺ T cells were sorted by miniMACS. The sorted CD4⁺ T cells were incubated with rhG-CSF for 24 h, then the adhesion and polarization of CD4⁺ T cells activated by stroma cell-derived factor -1α (SDF-1α) and ICAM-1 were detected by ELISA and inverted microscope. The results showed that the percentage of adhesion CD4⁺T cells in the experimental group (rhG-CSF acting on the healthy adult volunteers) (61.9 ± 5.9)% was lower than that in the control group (healthy adult volunteers without rhG-CSF stimulation) (68.3 ± 7.3)% (P < 0.05). The percentage of polarized CD4⁺T cells in the experimental group (24.3 ± 4.3)% was also lower than that in control group (47.1 ± 5.1)% (P < 0.05). It is concluded that the adhesion and polarization of CD4⁺T lymphocytes can be inhibited after rhG-CSF stimulation.
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Sha-Sha
authors.initials: SS
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zhen-Yang
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li-Xun
authors.initials: LX
authors.lastname: Guan
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Hematology, Chinese PLA Medical College, Chinese PLA General Hospital, Beijing 100853, China. E-mail: gaochunji@medmail.com.cn.
authors.forename: Chun-Ji
authors.initials: CJ
authors.lastname: Gao
mesh_terms: D000368:Aged; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement; D016764:Cell Polarity / Q000187:drug effects*; D054377:Chemokine CXCL12; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1; D007963:Leukocytes, Mononuclear; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1; D008875:Middle Aged; D011994:Recombinant Proteins
chemical_list: D054377:Chemokine CXCL12; D016169:Lymphocyte Function-Associated Antigen-1; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D016179:Granulocyte Colony-Stimulating Factor