title: Rapid evaluation of N-glycosylation status of antibodies with chemiluminescent lectin-binding assay.
abstract: We constructed an assay system for the rapid detection of the glycosylation status of antibodies with a chemiluminescence-based lectin-binding assay, and investigated the glycosylation dynamics during the culture of recombinant Chinese hamster ovary cells. This rapid detection system is applicable to the high-throughput evaluation of therapeutic glycoproteins.
authors.affiliation: Institute of Technology and Science, Tokushima University, Minamijosanjima-cho 2-1, Tokushima 770-8506, Japan. Electronic address: onitsuka@tokushima-u.ac.jp.
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Onitsuka
authors.affiliation: Institute of Technology and Science, Tokushima University, Minamijosanjima-cho 2-1, Tokushima 770-8506, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Omasa
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism*; D016466:CHO Cells; D003412:Cricetulus; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D049449:Luminescence; D008163:Luminescent Measurements; D011485:Protein Binding; D013997:Time Factors
chemical_list: D000906:Antibodies; D006023:Glycoproteins; D037102:Lectins
keywords: Antibody; Chemiluminescent assay; Chinese hamster ovary cells; Glycosylation; Lectin