title: Methanolic extract of Momordica cymbalaria enhances glucose uptake in L6 myotubes in vitro by up-regulating PPAR-γ and GLUT-4.
abstract: The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL(-1) were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL(-1)) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro.
authors.affiliation: Padmashree Institute of Information Sciences, Bangalore 560072, India. Electronic address: maheshguni@yahoo.com.
authors.forename: Puttanarasaiah Mahesh
authors.initials: PM
authors.lastname: Kumar
authors.affiliation: Connexios Life Sciences Pvt Ltd., J.P. Nagar, Bangalore 560078, India.
authors.forename: Marikunte V
authors.initials: MV
authors.lastname: Venkataranganna
authors.affiliation: Department of Microbiology and Biotechnology, Bangalore University, Bangalore 560056, India.
authors.forename: Kirangadur
authors.initials: K
authors.lastname: Manjunath
authors.affiliation: PES College of Pharmacy, Bangalore 560050, India.
authors.forename: Gollapalle L
authors.initials: GL
authors.lastname: Viswanatha
authors.affiliation: Director, Radiant Research Services Pvt Ltd., Bangalore 560050, India.
authors.forename: Godavarthi
authors.initials: G
authors.lastname: Ashok
mesh_terms: D001692:Biological Transport; D004305:Dose-Response Relationship, Drug; D005638:Fruit; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D029775:Momordica; D018485:Muscle Fibers, Skeletal / Q000187:drug effects*; D047495:PPAR gamma / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; D047495:PPAR gamma; D010936:Plant Extracts; D011500:Protein Synthesis Inhibitors; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D005947:Glucose
keywords: Antidiabetic activity; Diabetes; Glucose transport; L6 myotubes; Momordica cymbalaria; PPARγ