title: Plasmacytoid dendritic cell bactericidal activity against Burkholderia pseudomallei.
abstract: Melioidosis sepsis, caused by Burkholderia pseudomallei, is associated with high mortality due to an overwhelming inflammatory response. Plasmacytoid dendritic cells (pDC) are potent producers of type I interferons (IFN). This study investigated whether pDC and type I IFN play a role during the early stages of B. pseudomallei infection. Human and murine pDC internalised and killed B. pseudomallei as efficiently as murine conventional DC (cDC). pDC derived from B. pseudomallei-susceptible (BALB/c) mice demonstrated poor intracellular killing and increased IFN-alpha compared to pDC derived from B. pseudomallei-resistant (C57BL/6) mice. This is the first evidence of pDC bactericidal activity against B. pseudomallei infection.
authors.affiliation: Australian Institute of Tropical Health and Medicine, College of Public Health, Medicine and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia. Electronic address: Natasha.williams@my.jcu.edu.au.
authors.forename: Natasha L
authors.initials: NL
authors.lastname: Williams
authors.affiliation: Australian Institute of Tropical Health and Medicine, College of Public Health, Medicine and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia.
authors.forename: Jodie L
authors.initials: JL
authors.lastname: Morris
authors.affiliation: Australian Institute of Tropical Health and Medicine, College of Public Health, Medicine and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Rush
authors.affiliation: Australian Institute of Tropical Health and Medicine, College of Public Health, Medicine and Veterinary Sciences, James Cook University, Townsville, Queensland 4811, Australia.
authors.forename: Natkunam
authors.initials: N
authors.lastname: Ketheesan
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000166:cytology / Q000502:physiology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004195:Disease Models, Animal; D016898:Interferon-alpha; D016899:Interferon-beta; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D016898:Interferon-alpha; D016899:Interferon-beta
keywords: Burkholderia pseudomallei; Dendritic cells; Interferon-alpha; Interferon-beta; Melioidosis