title: Monitoring Bacteroides spp. markers, nutrients, metals and Escherichia coli in soil and leachate after land application of three types of municipal biosolids.
abstract: A lysimeter-based field study was done to monitor the transfer of culturable Escherichia coli, general (ALLBAC), human (Hf183) and swine (PIG-BAC-1) specific 16S rRNA Bacteroides spp. markers, nutrients and metals through soils and leachate over time following land application of a CP1/Class A as well as two CP2/Class B municipal biosolids (MBs). Hf183 markers were detected up to six days following application in soils receiving dewatered and liquid MBs, but not in leachate, suggesting their use in source tracking is better suited for recent pollution events. The CP2/Class B biosolids and swine manure contributed the highest microbial load with E. coli loads (between 2.5 and 3.7 log CFU (100 mL)(-1)) being greater than North American concentration recommendations for safe recreational water. ALLBAC persisted in soils and leachate receiving all treatments and was detected prior to amendment application demonstrating its unsuitability for identifying the presence of fecal pollution. A significant increase in NO₃-N (for Lystek and dewatered MBs) and total-P (for dewatered and liquid MBs) in leachate was observed in plots receiving the CP1/Class A and CP2/Class B type MBs which exceeded North American guidelines, suggesting impact to surface water. Metal (As, Cd, Cr, Co, Cu, Pb, Mo, Ni, Se, Zn and Hg) transfer was negligible in soil and leachate samples receiving all treatments. This study is one of the first to examine the fate of E. coli and Bacteroides spp. markers in situ following the land application of MBs where surface runoff does not apply.
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Crystal A
authors.initials: CA
authors.lastname: McCall
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Katerina S
authors.initials: KS
authors.lastname: Jordan
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada.
authors.forename: Marc B
authors.initials: MB
authors.lastname: Habash
authors.affiliation: School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2W1, Canada. Electronic address: dunfield@uoguelph.ca.
authors.forename: Kari E
authors.initials: KE
authors.lastname: Dunfield
mesh_terms: D000818:Animals; D001439:Bacteroides / Q000302:isolation & purification*; D015415:Biomarkers / Q000032:analysis; D004784:Environmental Monitoring; D004926:Escherichia coli / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D006801:Humans; D008670:Metals / Q000032:analysis; D009864:Ontario; D012621:Seasons; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*; D062611:Solid Waste / Q000032:analysis; D034421:Sus scrofa; D014131:Trace Elements / Q000032:analysis; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D015415:Biomarkers; D008670:Metals; D012989:Soil Pollutants; D062611:Solid Waste; D014131:Trace Elements; D014874:Water Pollutants, Chemical
keywords: Bacteroides spp.; Fecal contaminants; Fecal indicator organisms; Fecal source tracking; qPCR