title: Comparison of two different reactive dye immobilized poly(hydroxyethyl methacrylate) cryogel discs for purification of lysozyme.
abstract: In this study, cibacron blue F3GA and alkali blue 6B immobilized poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were prepared. The cryogel discs were characterised by swelling tests, elemental analysis and scanning electron microscopy. Each cryogel disc was used for lysozyme adsorption from aqueous solutions. Maximum adsorption capacities were 103.3 and 106.7 mg/g for cibacron blue F3GA and alkali blue 6B immobilized cryogel discs, respectively. Equilibrium lysozyme concentration, pH, ionic strength and temperature were the factors of which effect on lysozyme adsorption was investigated. Reusability of the cryogel discs was tested and less than 5% decrease in adsorption capacity was reported. In the last stage of this work, the cryogel discs were used for lysozyme purification from chicken egg white. Molecular weight and purity of the eluted lysozyme from cryogel discs were controlled by sodium dodecyl sulfate polyacrylamide gel electrophoresis. In conclusion, cibacron blue F3GA and alkali blue 6B immobilized cryogel discs present a cheap and fast way for purification of lysozyme with a high purity.
authors.affiliation: Department of Biology, Molecular Biology Division, Hacettepe University, Beytepe, 06800, Ankara, Turkey.
authors.forename: Işık
authors.initials: I
authors.lastname: Perçin
authors.forename: Gulsu
authors.initials: G
authors.lastname: Sener
authors.forename: Ahmet Hamdi
authors.initials: AH
authors.lastname: Demirçelik
authors.forename: Nilay
authors.initials: N
authors.lastname: Bereli
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D001557:Benzenesulfonates / Q000737:chemistry*; D002645:Chickens; D004396:Coloring Agents / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D004529:Egg White / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D013194:Staining and Labeling / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D014227:Triazines / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D001557:Benzenesulfonates; D004396:Coloring Agents; D059985:Cryogels; D014227:Triazines; D011102:Polyhydroxyethyl Methacrylate; C111519:alkali blue 6B; C012842:Cibacron Blue F 3GA; D009113:Muramidase