title: Genome-scale transcriptome analysis in response to nitric oxide in birch cells: implications of the triterpene biosynthetic pathway.
abstract: Evidence supporting nitric oxide (NO) as a mediator of plant biochemistry continues to grow, but its functions at the molecular level remains poorly understood and, in some cases, controversial. To study the role of NO at the transcriptional level in Betula platyphylla cells, we conducted a genome-scale transcriptome analysis of these cells. The transcriptome of untreated birch cells and those treated by sodium nitroprusside (SNP) were analyzed using the Solexa sequencing. Data were collected by sequencing cDNA libraries of birch cells, which had a long period to adapt to the suspension culture conditions before SNP-treated cells and untreated cells were sampled. Among the 34,100 UniGenes detected, BLASTX search revealed that 20,631 genes showed significant (E-values≤10-5) sequence similarity with proteins from the NR-database. Numerous expressed sequence tags (i.e., 1374) were identified as differentially expressed between the 12 h SNP-treated cells and control cells samples: 403 up-regulated and 971 down-regulated. From this, we specifically examined a core set of NO-related transcripts. The altered expression levels of several transcripts, as determined by transcriptome analysis, was confirmed by qRT-PCR. The results of transcriptome analysis, gene expression quantification, the content of triterpenoid and activities of defensive enzymes elucidated NO has a significant effect on many processes including triterpenoid production, carbohydrate metabolism and cell wall biosynthesis.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin 150040, China; College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Fansuo
authors.initials: F
authors.lastname: Zeng
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Fengkun
authors.initials: F
authors.lastname: Sun
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), Harbin 150040, China; College of Life Science, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Zhan
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D029662:Betula / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D009828:Oleanolic Acid / Q000378:metabolism; D059828:Plant Cells / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D014315:Triterpenes / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014315:Triterpenes; D009599:Nitroprusside; D009569:Nitric Oxide; D009828:Oleanolic Acid