title: Expression of claudins in human clear cell renal cell carcinoma.
abstract: The current study investigated the expression of claudins 1, 5 and 16 in human clear cell renal cell carcinoma (CCRCC) and the impact of claudin-16 on kidney cancer cells. Levels of claudin transcripts were assessed using quantitative reverse transcription-polymerase reaction and proteins were examined by immunohistochemical methods. Human kidney epithelial cell HEK293 and human kidney cancer cell line UMRC2 were also used in the study. Human kidney tissues expressed high levels of claudin-1 and in particular claudin-16, but were negative for claudin-5. Late-stage kidney carcinomas tended to express low levels of claudin-1 and claudin-16. UMRC2 cells, highly positive for claudin-16, were transfected with an anti-claudin-16 transgene, which resulted in significant reduction in both transepithelial resistance and cell matrix adhesion compared to control cells. When HEK293 cells, negative for expression of claudin-16, were forced to express claudin-16, the cells showed a marked increase in transepithelial resistance and to some degree in cell matrix adhesion. In conclusion, the present study showed that kidney tissue and CCRCC tissues highly expressed claudin-16 and claudin-1, and that claudin-16 has a profound impact on barrier function and cell adhesion in kidney cancer cells.
authors.affiliation: Department of Nephrology, Ji Shui Tan Hospital, Beijing, P.R. China Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Men
authors.affiliation: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Tracey A
authors.initials: TA
authors.lastname: Martin
authors.affiliation: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Fiona
authors.initials: F
authors.lastname: Ruge
authors.affiliation: Cardiff University-Capital Medical University Joint Centre for Biomedical Research, Beijing ChaoYang Hospital, Capital Medical University, Beijing, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Peking University Cancer Hospital and School of Oncology, Beijing, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: DU
authors.affiliation: Peking University Cancer Hospital and School of Oncology, Beijing, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Cardiff University-Peking University Cancer Institute, Cardiff University School of Medicine, Cardiff, U.K.
authors.forename: Wen G
authors.initials: WG
authors.lastname: Jiang
mesh_terms: D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D057167:Claudins / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism*; D014162:Transfection
chemical_list: D057167:Claudins
keywords: Claudin-1; claudin-16; claudin-5; clear cell renal cell carcinoma; paracellular permeability