title: Antimicrobial activity of human β-defensins against lactic acid bacteria.
abstract: In this study, we evaluated the antimicrobial activity of human β-defensin-1 (hBD-1), human β-defensin-2 (hBD-2) and human β-defensin-3 (hBD-3) against three internationally common probiotic strains of lactic acid bacterium. Our results indicated that hBD-1, hBD-2 and hBD-3 at the range of 0.08-10 μg/mL do not have obvious antimicrobial activity against these strains. Viability of Bifidobacterium longum JDM301 (B. longum JDM301), Bifidobacterium lactis HN019 (B. lactis HN019) and Lactobacillus rhamnosus GG (LGG) were still very high even at concentration of 10 μg hBD/mL. Then, we explored the mechanism of resistance by using carbonyl cyanide 3-chlorophenylhydrazone (CCCP) to inhibit efflux pumps. In the presence of CCCP, hBD-1, hBD-2 and hBD-3 exhibited enhanced antibacterial effect against B. longum JDM301 and B. lactis HN019, but not against LGG. Efflux pumps in B. longum JDM301 and B. lactis HN019 may partly contribute to their resistance to hBD-1, hBD-2, and hBD-3.
authors.affiliation: a Department of Allergy and Immunology , Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.|b Division of Immunology, Institute of Pediatric Translational Medicine, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Wang
authors.affiliation: c Department of Medical Microbiology and Parasitology , Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine , Shanghai 200025 , P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.affiliation: d Department of Pediatrics , Xinhua Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai 200092 , P.R. China.
authors.forename: Rui-Ming
authors.initials: RM
authors.lastname: Cao
authors.affiliation: e Beingmate Research Center for Human Breast Milk, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: e Beingmate Research Center for Human Breast Milk, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.
authors.forename: Sheng-Mei
authors.initials: SM
authors.lastname: Wu
authors.affiliation: c Department of Medical Microbiology and Parasitology , Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine , Shanghai 200025 , P.R. China.
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Guo
authors.affiliation: a Department of Allergy and Immunology , Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.|b Division of Immunology, Institute of Pediatric Translational Medicine, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.|f Department of Nephrology and Rheumatology , Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine , Shanghai 200127 , P.R. China.
authors.forename: Tong-Xin
authors.initials: TX
authors.lastname: Chen
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry; D001644:Bifidobacterium; D006801:Humans; D007778:Lactobacillus; D019936:Probiotics; D023083:beta-Defensins / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; C406407:DEFB1 protein, human; C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D023083:beta-Defensins
keywords: antimicrobial peptide; defensin; efflux pump; human β-defensin; probiotics