title: [Protection of T-type calcium channel blocker in spiral ganglion neurons of adult C57BL/6J mice].
abstract: OBJECTIVE
  To explore the distribution and expression of three T-type calcium channel receptors (α1G; α1H; α1I) and understand their protective effects in spiral neurons of C57BL/6J mice.
  
  
  METHODS
  The distribution and expression of three T-type calcium channel receptors in spiral ganglion neurons were observed by in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR) in 6-8-week-old C57BL/6J mice. The mice of 24-26-week-old C57BL/6J were divided into 3 groups of zonisamide, benidipine and saline. And the expression changes of calcium-binding proteins calmodulin and calbindin were observed by immunohistochemistry.
  
  
  RESULTS
  Three subunits were expressed in spiral ganglion neurons. The decremented quantities were α1H (24.21 ± 0.10), α1I (14.88 ± 0.04) and α1G (10.42 ± 0.02). The expression level of calmodulin in spiral ganglion neurons was lower in the zonisamide-treated group than that in the saline-treated group (0.336 ± 0.041 vs 0.504 ± 0.020, P < 0.05). The expression level of calbindin in spiral ganglion neurons was lower in the zonisamide (0.482 ± 0.045) and benidipine-treated groups (0.511 ± 0.032) than that in the saline-treated group (0.611 ± 0.035, P < 0.05).
  
  
  CONCLUSIONS
  The expressions of calcium-binding proteins decrease after 4-week dosing of T-type calcium channel blockers in 24-26-week C57BL/6J mice. It implies a relief of calcium overload. T-type calcium channel blockers may protect the murine spiral ganglion neurons from degeneration.
authors.affiliation: Department of Otorhinolaryngology, First Affiliated Hospital, Soochow University, Suzhou 215006, China. Email: yfyu1024@163.com.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Yu
authors.forename: Chen
authors.initials: C
authors.lastname: Pan
authors.forename: Hongyang
authors.initials: H
authors.lastname: Ling
authors.forename: Wenying
authors.initials: W
authors.lastname: Wu
authors.forename: Gensheng
authors.initials: G
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D020747:Calcium Channels, T-Type; D002135:Calcium-Binding Proteins; D017403:In Situ Hybridization; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons; D013136:Spiral Ganglion
chemical_list: D002121:Calcium Channel Blockers; D020747:Calcium Channels, T-Type; D002135:Calcium-Binding Proteins