title: A conservative mutant of a proteolytic fragment produced during fibril formation enhances fibrillogenesis.
abstract: The fibrillogenesis of a peptide corresponding to residues 35-51 of human α-lactalbumin (¹GYDTQAIVENNESTEYG¹⁷) can be dramatically enhanced by the addition of a tetrapeptide TDYG homologous to its C-terminus (TEYG). Generation of spontaneous hydrolytic products similar to this peptide was demonstrated by mass-spectrometry analysis of GYDTQAIVENNESTEYG peptide solution components during fibrillogenesis. Possible mechanisms and roles of short peptides in protein metabolism are discussed.
authors.affiliation: a FSBI Research Institute of Influenza; Ministry of Healthcare of the Russian Federation ; St. Petersburg , Russia.
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Egorov
authors.forename: Dmitry V
authors.initials: DV
authors.lastname: Lebedev
authors.forename: Aram A
authors.initials: AA
authors.lastname: Shaldzhyan
authors.forename: Alexey K
authors.initials: AK
authors.lastname: Sirotkin
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Gorshkov
authors.forename: Olga A
authors.initials: OA
authors.lastname: Mirgorodskaya
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Grudinina
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Vasin
authors.forename: Michael M
authors.initials: MM
authors.lastname: Shavlovsky
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D006801:Humans; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D009154:Mutation; D059748:Proteolysis
chemical_list: D000682:Amyloid
keywords: Fibrillogenesis; GYDTQAIVENNESTEYG; conformation transmission; lactalbumin; short peptides; spontaneous hydrolysis