title: Development of transgenic mouse model expressing porcine aminopeptidase N and its susceptibility to porcine epidemic diarrhea virus.
abstract: Porcine coronavirus infections have known as they are specific to pigs with predominantly enteric or respiratory diseases. No laboratory animal model is yet been developed in porcine coronaviruses study. Here, we report that development of a transgenic mouse model expressing porcine APN which is susceptible to porcine coronavirus infection. The porcine APN transgene was constructed by fusing with mouse proximal APN promoter at 5' terminus and bovine growth hormone polyadenylation site at its 3' terminus. After screen on pubs from the microinjected mice, we confirmed two transgenic lines expressing porcine APN in various organs. We confirmed the susceptibility to porcine epidemic diarrhea virus, one of the porcine coronaviruses. These transgenic mice will be an important tool for research into the porcine coronaviruses.
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Department of Microbiology & Molecular Biology, College of Bioscience & Biotechnology, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Eui-Soon
authors.initials: ES
authors.lastname: Park
authors.affiliation: Department of Microbiology & Molecular Biology, College of Bioscience & Biotechnology, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yu
authors.affiliation: Department of Microbiology & Molecular Biology, College of Bioscience & Biotechnology, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Jaerang
authors.initials: J
authors.lastname: Rho
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea.
authors.forename: Sarita
authors.initials: S
authors.lastname: Paudel
authors.affiliation: Animal and Plant Quarantine Agency, 175, Anyang-ro, Manan-gu, Anyang, Gyeonggi-do, Republic of Korea.
authors.forename: Bang-Hun
authors.initials: BH
authors.lastname: Hyun
authors.affiliation: Animal and Plant Quarantine Agency, 175, Anyang-ro, Manan-gu, Anyang, Gyeonggi-do, Republic of Korea.
authors.forename: Dong-Kun
authors.initials: DK
authors.lastname: Yang
authors.affiliation: Laboratory of Infectious Diseases, College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea; Research Institute of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea. Electronic address: shin0089@cnu.ac.kr.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Shin
mesh_terms: D000818:Animals; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism*; D018352:Coronavirus Infections / Q000473:pathology* / Q000821:virology; D004195:Disease Models, Animal; D008822:Mice, Transgenic; D053485:Porcine epidemic diarrhea virus / Q000502:physiology*; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D013552:Swine
chemical_list: D011991:Receptors, Virus; D018826:CD13 Antigens
keywords: Pathogenesis; Porcine APN; Porcine coronaviruses; Porcine epidemic diarrhea virus; Transgenic mouse model