title: miR-374a promotes cell proliferation, migration and invasion by targeting SRCIN1 in gastric cancer.
abstract: MicroRNAs (miRNAs) play a prominent role in gastric cancer (GC) initiation and progression. In this study, we found that miR-374a expression was up-regulated in human GC cell lines and tissues. Inhibition of miR-374a suppressed GC cell proliferation, migration and invasion in vitro and slowed tumor growth in vivo. SRC kinase signaling inhibitor 1 (SRCIN1) was identified as a direct target of miR-374a. Silencing of SRCIN1 significantly enhanced cell proliferation, migration and invasion, whereas SRCIN1 reintroduction partially abrogated the oncogenic effects of miR-374a. Taken together, these findings suggest that miR-374a functions as a candidate oncogene in GC by directly targeting SRCIN1. miR-374a may therefore be useful as a promising therapeutic target for malignant GC.
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Xinyun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Su
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Xujun
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Gao
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China.
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of General Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, PR China. Electronic address: sunyanpingcz2003@sina.com.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Sun
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000096:biosynthesis* / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; C587995:MIRN374 microRNA 374, human; D035683:MicroRNAs; C532615:SRCIN1 protein, human
keywords: Gastric cancer; SRC kinase signaling inhibitor 1; miR-374a