title: Gelsolin bound β-amyloid peptides(1-40/1-42): electrochemical evaluation of levels of soluble peptide associated with Alzheimer's disease.
abstract: A method for the highly sensitive determination of soluable β-amyloid peptides (Aβ(1-40/1-42)) that employs a detection bioconjugate of HRP-Au-gelsolin as the electrochemical nanoprobe is presented. Contrary to previous detection notions that utilized antibodies, which could specifically recognize the N- or C-terminus of peptides, we demonstrate herein that the reported specific binding between gelsolin and Aβ might provide an alternative way to evaluate the peptides sensitively and selectively. The HRP-Au-gelsolin nanohybrid was designed by one-pot functionalization of Au nanaoparticles (NPs) with horseradish peroxidase (HRP) and gelsolin. Through a sandwich-type sensor array, soluble Aβ(1-40/1-42) were captured onto the array due to the interactions between targeted peptides and surface-confined gelsolin and electrochemical signals were amplified by abundant attachments of HRP labeled on AuNPs, which could specifically catalyse its substrate, 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to give rise to measurable signals. The proposed gelsolin-bound Aβ methodology displayed satisfactory sensitivity and wide linear range towards Aβ(1-40/1-42) with a detection limit down to 28 pM, which are verified to be sensitive-enough for the assessment of Aβ levels both in normal and Alzheimer's disease (AD) rat brains. Experimental results indicated that compared with normal group, soluble β-amyloid peptide levels in cerebrospinal fluid (CSF) and targeted brain tissues of AD rats all declined with differentiable degrees. In short, the newly unfolding strategy presents valuable information related to pathological events in brain and will exhibit a braw perspective for the early diagnosis of AD process.
authors.affiliation: Department of Pharmaceutical Analysis, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Analysis, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, PR China; Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China.
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Agro-food Standards and Testing Technologies, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, PR China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Nie
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, 209 Tongshan Road, Xuzhou 221004, Jiangsu, PR China. Electronic address: yinxx@xzmc.edu.cn.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai 200241, PR China. Electronic address: gyshi@chem.ecnu.edu.cn.
authors.forename: Guoyue
authors.initials: G
authors.lastname: Shi
mesh_terms: D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D000818:Animals; D015374:Biosensing Techniques; D018260:Gelsolin / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D051381:Rats
chemical_list: D016229:Amyloid beta-Peptides; D018260:Gelsolin; D006046:Gold; D006861:Hydrogen Peroxide
keywords: AD; Aβ(1–40/1–42); Electrochemistry; Gelsolin