title: Antimicrobial activity of different solvent extracted samples from the flowers of medicinally important Plumeria obstusa.
abstract: The present research work was carried out to investigate the antimicrobial (eight bacteria and one fungus) activities of different solvent (ethanol, petroleum ether, chloroform, ethyl acetate and isobutanol) extracted samples from flowers of P. obstusa by disc diffusion method. Analysis of the data revealed that all the five extracts from flowers of P. obstusa showed different ranges of antimicrobial activities. Petroleum ether fractions showed inhibitory activities against all the nine microbial species except Klebsiella pneumonia. Ethyl acetate and isobutanol fractions showed inhibitory effects against all the tested microbial species except Pseudomonas aeruginosa. Chloroform and ethanol extracts had varying levels of inhibitions against all of the tested microorganisms. The most susceptible gram positive bacterium was Bacillus subtilis which was inhibited by all the five extracts while the most resistant gram positive bacterium was Staphylococcus aureus. Erwinia carotovora was the most susceptible gram negative bacterium while Pseudomonas aeruginosa was highly resistant among the gram negative bacteria.
authors.affiliation: Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Nasir
authors.initials: N
authors.lastname: Ali
authors.affiliation: Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Dawood
authors.initials: D
authors.lastname: Ahmad
authors.affiliation: Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
authors.forename: Jehan
authors.initials: J
authors.lastname: Bakht
mesh_terms: D000085:Acetates / Q000737:chemistry; D000473:Alkanes / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D029062:Apocynaceae / Q000737:chemistry; D000440:Butanols / Q000737:chemistry; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D002725:Chloroform / Q000737:chemistry; D052978:Disk Diffusion Antimicrobial Tests; D000431:Ethanol / Q000737:chemistry; D035264:Flowers; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry*
chemical_list: D000085:Acetates; D000473:Alkanes; D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000440:Butanols; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; C040507:isobutyl alcohol; C007650:ethyl acetate; D002725:Chloroform; C004544:naphtha