title: Lactococcus lactis displayed neuraminidase confers cross protective immunity against influenza A viruses in mice.
abstract: Influenza A viruses pose a serious threat to public health. Current influenza A vaccines predominantly focus on hemagglutinin (HA) and show strain-specific protection. Neuraminidase (NA) is much less studied in the context of humoral immunity against influenza A viruses. The purpose of this study is to evaluate the cross protective immunity of NA presented on Lactococcus lactis (L.lactis) surface against homologous and heterologous influenza A viruses in the mouse model. L.lactis/pNZ8110-pgsA-NA was constructed in which pgsA was used as an anchor protein. Mice vaccinated orally with L.lactis/pNZ8110-pgsA-NA could elicit significant NA-specific serum IgG and mucosa IgA antibodies, as well as neuraminidase inhibition (NI) titers. Importantly, L.lactis/pNZ8110-pgsA-NA provided 80% protection against H5N1, 60% protection against H3N2 and H1N1, respectively. These findings suggest that recombinant L.lactis/pNZ110-pgsA-NA in the absence of adjuvant via oral administration can be served as an effective vaccine candidate against diverse strains of influenza A viruses.
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China; Department of Biomedical Engineering, State University of New York, Binghamton, 13902, USA. Electronic address: hlei@binghamton.edu.
authors.forename: Han
authors.initials: H
authors.lastname: Lei
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Xiaojue
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Daxian
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Jiexiu
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Jiao
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Handing
authors.initials: H
authors.lastname: Shu
authors.affiliation: Department of Biotechnology, College of Life Science, Nanchang University, Jiangxi 330031, China.
authors.forename: Xinqi
authors.initials: X
authors.lastname: Ge
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D056738:Cross Protection; D005260:Female; D015870:Gene Expression; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology* / Q000502:physiology; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000502:physiology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology* / Q000502:physiology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology* / Q000821:virology; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase
keywords: Cross protective immunity; L.lactis/pNZ8110-pgsA-NA; Neuraminidase (NA)