title: Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides.
abstract: To understand the function of certain RNAs, including noncoding RNAs, it is important to identify the proteins that interact with the RNAs. Here we describe the method for purification of ribonucleoprotein (RNP) complexes composed of specific cellular RNAs by pull-down with FLAG peptide-conjugated antisense oligonucleotide (ASO). Using this method, we identified a novel protein component of U7 snRNP complex.
authors.affiliation: Molecular Profiling Research Center for Drug Discovery (molprof), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo, 135-0064, Japan.
authors.forename: Shungo
authors.initials: S
authors.lastname: Adachi
authors.forename: Tohru
authors.initials: T
authors.lastname: Natsume
mesh_terms: D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry; D011485:Protein Binding; D022661:RNA, Untranslated / Q000302:isolation & purification* / Q000378:metabolism*; D020885:Ribonucleoprotein, U7 Small Nuclear / Q000378:metabolism*
chemical_list: D016376:Oligonucleotides, Antisense; D009842:Oligopeptides; D022661:RNA, Untranslated; D020885:Ribonucleoprotein, U7 Small Nuclear; C085706:FLAG peptide