title: Application of glyco-blotting for identification of structures of polysaccharides causing membrane fouling in a pilot-scale membrane bioreactor treating municipal wastewater.
abstract: A new approach for the analysis of polysaccharides in membrane bioreactor (MBR) is proposed in this study. Enrichment of polysaccharides by glyco-blotting, in which polysaccharides are specifically collected via interactions between the aldehydes in the polysaccharides and aminooxy groups on glycoblotting beads, enabled MALDI-TOF/MS analysis at a high resolution. Structures of polysaccharides extracted from fouled membranes used in a pilot-scale MBR treating municipal wastewater and those in the supernatant of the mixed liquor suspension in the MBR were investigated. It was found that the overlap between polysaccharides found in the supernatants and those extracted from the fouled membrane was rather limited, suggesting that polysaccharides that dominate in supernatants may not be important in membrane fouling in MBRs. Analysis using a bacterial carbohydrate database suggested that capsular polysaccharides (CPS) and/or lipo-polysaccharides (LPS) produced by gram-negative bacteria are key players in the evolution of membrane fouling in MBRs.
authors.affiliation: Division of Environmental Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan. Electronic address: kkatsu@eng.hokudai.ac.jp.
authors.forename: Katsuki
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Graduate School of Life Science, Hokkaido University, Sapporo 001-0012, Japan.
authors.forename: Shin-Ichiro
authors.initials: SI
authors.lastname: Nishimura
authors.affiliation: Medicinal Chemistry Pharmaceuticals, LLC, 1-715, N7W4, Kita-ku, Sapporo 060-0807, Japan.
authors.forename: Risho
authors.initials: R
authors.lastname: Miyoshi
authors.affiliation: Division of Environmental Engineering, Graduate School of Engineering, Hokkaido University, Sapporo 060-8628, Japan.
authors.forename: Asiful
authors.initials: A
authors.lastname: Hoque
authors.affiliation: Center for Environmental Nano and Bio Engineering, Hokkaido University, Sapporo 060-8628, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Miyoshi
authors.affiliation: Center for Environmental Nano and Bio Engineering, Hokkaido University, Sapporo 060-8628, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D057219:Biofouling; D019149:Bioreactors; D002947:Cities; D006868:Hydrolysis; D008567:Membranes, Artificial; D010865:Pilot Projects; D011134:Polysaccharides / Q000737:chemistry*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D062065:Wastewater / Q000737:chemistry*; D018508:Water Purification / Q000295:instrumentation* / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011134:Polysaccharides; D062065:Wastewater
keywords: Capsular polysaccharides; Glyco-blotting; Lipo-polysaccharides; MALDI-TOF/MS; Wastewater treatment