title: Effects of tobacco smoking during pregnancy on oxidative stress in the umbilical cord and mononuclear blood cells of neonates.
abstract: BACKGROUND
  Although cigarette smoke is known to be a complex mixture of over 4000 substances that can lead to damage through active or passive smoking, its mechanisms and biochemical consequences in pregnancy and neonates are not yet fully understood. Therefore, in the present study, we propose to study the impact of smoking during gestation on the viability of blood mononuclear cells (MNC) from umbilical cords of newborns to assess the degree of oxidative stress and cell viability. After childbirth, the cord blood and the umbilical cord were immediately collected in public hospitals in Greater Vitoria, ES, Brazil. Flow cytometry was used to analyze the cord blood followed by biochemical and histological tests to analyze possible changes in the umbilical cord.
  
  
  RESULTS
  Pregnant smokers had a reduction of MNC viability from the umbilical cord (10%), an increase in the production of reactive oxygen species (ROS) and an increase in cell apoptosis (~2-fold) compared to pregnant non-smokers. In the umbilical cord, it was observed an increase of advanced oxidation protein products - AOPP (~2.5-fold) and a loss of the typical architecture and disposition of endothelial cells from the umbilical artery.
  
  
  CONCLUSIONS
  These data suggest that maternal cigarette smoking during pregnancy (even in small amounts) may compromise the viability of MNC cells and damage the umbilical cord structure, possibly by excessive ROS bioavailability.
authors.forename: Ednildes de Almeida Olympio
authors.initials: Ede A
authors.lastname: Rua
authors.forename: Marcella Leite
authors.initials: ML
authors.lastname: Porto
authors.forename: Jean Pierre Louzada
authors.initials: JP
authors.lastname: Ramos
authors.forename: Breno Valentim
authors.initials: BV
authors.lastname: Nogueira
authors.forename: Silvana S
authors.initials: SS
authors.lastname: Meyrelles
authors.forename: Elisardo Corral
authors.initials: EC
authors.lastname: Vasquez
authors.forename: Thiago C
authors.initials: TC
authors.lastname: Pereira
mesh_terms: D000328:Adult; D001938:Brazil; D005260:Female; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005333:Fetus / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000473:pathology*; D018384:Oxidative Stress; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced*; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D055815:Young Adult
chemical_list: D014028:Tobacco Smoke Pollution