title: [Hesperetin attenuates angiotensin II-induced collagen expression in cardiac fibroblasts in vitro].
abstract: OBJECTIVE
  To explore the effect of hesperetin (HES) on collagen expression in cardiac fibroblasts in vitro induced by angiotensin II (AngII).
  
  
  METHODS
  Cell growth was determined by trypan blue staining, reactive oxygen species (ROS) generation by microplate reader, the expressions of collagen I,III and matrix metalloproteinase 1 (MMP-1) by real-time polymerase chain reaction (PCR) and cell proliferation by cell counting kit-8.
  
  
  RESULTS
  HES and AngII+HES had no effect on cellular activity. AngII significantly increased ROS generation (1.70 ± 0.12 vs 1, P < 0.01), gene expression of collagen I,III both increased (1.31 ± 0.08 vs 1, 1.40 ± 0.09 vs 1, both P < 0.01) while MMP-1 decreased (0.68 ± 0.03 vs 1, P < 0.01). Ang II also induced the proliferation of fibroblasts (1.91 ± 0.18 vs 1, P < 0.01). While HES (25, 50, 100 µmol/L) or NAC (1 mmol/L) reversed these effects during co-treating with AngII, ROS decreased versus the Ang II group(1.37 ± 0.05, 1.16 ± 0.08, 1.07 ± 0.07, 1.12 ± 0.07 vs 1.70 ± 0.12, all P < 0.01) , gene expression of collagenI,III also decreased (1.22 ± 0.08 and 1.27 ± 0.07, 1.14 ± 0.07 and 1.00 ± 0.06, 1.02 ± 0.06 and 0.99 ± 0.05, 1.08 ± 0.07 and 1.09 ± 0.06 vs 1.31 ± 0.08 and 1.40 ± 0.09, all P < 0.01), MMP-1 increased (0.76 ± 0.05, 0.88 ± 0.07, 1.01 ± 0.08, 0.96 ± 0.07 vs 0.68 ± 0.03, P < 0.01) versus the Ang II group. Cell proliferation was also inhibited (1.42 ± 0.07, 1.38 ± 0.03, 1.07 ± 0.15, 1.16 ± 0.11 vs 1.91 ± 0.18, all P < 0.01). NAC had the same effect with HES.
  
  
  CONCLUSIONS
  HES inhibits the synthesis of collagen and the Ang II-induced proliferation of fibroblast through suppressing the ROS generation.
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China. Email: qztang@whu.edu.cn.
authors.forename: Qizhu
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiao
authors.affiliation: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Deng
mesh_terms: D000804:Angiotensin II; D049109:Cell Proliferation; D003094:Collagen; D005347:Fibroblasts; D015870:Gene Expression; D006321:Heart; D006569:Hesperidin; D066298:In Vitro Techniques; D020781:Matrix Metalloproteinase 1; D017382:Reactive Oxygen Species
chemical_list: D017382:Reactive Oxygen Species; D000804:Angiotensin II; D003094:Collagen; D006569:Hesperidin; D020781:Matrix Metalloproteinase 1; C013015:hesperetin