title: Preoperative exercise facilitates abundant bone marrow collection in patients with type 2 diabetes for mononuclear cell therapy.
abstract: OBJECTIVE
  Traditional bone marrow (BM) collection is inadequate for separation of abundant mononuclear cells (MNCs). We aimed to investigate the effects of preoperative exercise on BM collection in patients with type 2 diabetes mellitus (T2DM).
  
  
  METHODS
  Sixty patients with T2DM were randomly assigned to either a control group or an exercise group (n = 30 each). The patients in the exercise group exercised before the collection. All patients underwent routine surgical care. The collected BM volume, operation duration, collecting speed, puncture times and pain scores were recorded. BM samples were tested before and after MNCs separation for CD34+ flow cytometry and whole blood cell count.
  
  
  RESULTS
  The collected BM volumes were significantly larger and collection speed was faster in the exercise group (379.77 ± 4.93 mL and 1.40 ± 0.14 mL/s) than those in the control group (356.67 ± 15.36 mL and 0.89 ± 0.16 mL/s, P = 0.00 for both). Puncture times were significantly less and pain scores were lower in the exercise group (2.07 ± 0.25 and 2.67 ± 1.56) than those in the control group (2.50 ± 0.63 and 3.43 ± 1.76, P = 0.00 and 0.02, respectively). CD34+ cells and whole blood cell count variables were comparable in the 2 groups.
  
  
  CONCLUSIONS
  Preoperative exercise facilitates BM collection by increasing collected volume, improving collecting speed, relieving patients' pains and ensuring MNC quality.
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou, People's Republic of China.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Luo
authors.affiliation: Obstetrics and Gynecology Department, Quanzhou First Hospital, Quanzhou, People's Republic of China.
authors.forename: Zhifen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou, People's Republic of China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Tao
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou, People's Republic of China.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zou
authors.affiliation: Organ Transplant Institute, Fuzhou General Hospital (Dongfang Hospital), Xiamen University, Fuzhou, People's Republic of China. Electronic address: doctortjm@yahoo.com.
authors.forename: Jianming
authors.initials: J
authors.lastname: Tan
mesh_terms: D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D064987:Cell- and Tissue-Based Therapy; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology* / Q000628:therapy; D015444:Exercise / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology* / Q000637:transplantation; D011300:Preoperative Care
keywords: bone marrow; exercise; mononuclear cells; type 2 diabetes