title: ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide.
abstract: This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. Sequencing reads can then be used to infer regions of increased accessibility, as well as to map regions of transcription-factor binding and nucleosome position. The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assaying nucleosome positions in accessible regions of the genome.
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California.|Program in Epithelial Biology and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California.
authors.forename: Jason D
authors.initials: JD
authors.lastname: Buenrostro
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California.
authors.forename: Beijing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Program in Epithelial Biology and the Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California.
authors.forename: Howard Y
authors.initials: HY
authors.lastname: Chang
authors.affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, California.
authors.forename: William J
authors.initials: WJ
authors.lastname: Greenleaf
mesh_terms: D002843:Chromatin / Q000235:genetics* / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D008967:Molecular Biology / Q000379:methods*; D011485:Protein Binding; D011995:Recombination, Genetic; D013194:Staining and Labeling / Q000379:methods*; D019895:Transposases / Q000378:metabolism
chemical_list: D002843:Chromatin; D019895:Transposases
keywords: ATAC-seq; chromatin accessibility; transposase