title: Solvothermal synthesis of ZnO nanoparticles and anti-infection application in vivo.
abstract: Zinc oxide nanoparticles (ZnONPs) have been widely studied as the bacteriostatic reagents. However, synthesis of small ZnO nanoparticles with good monodispersion and stability in aqueous solution is still a challenge. Anti-infection research of ZnONPs used as antibacterial agent in vivo is rare. In this paper, a novel, sustainable, and simple method to synthesize ZnO nanoparticles with good monodispersion in aqueous low-temperature conditions and with a small molecule agent is reported. Inhibition zone test and the minimum inhibitory concentration test were performed to examine the antibacterial activity of ZnONPs against bacteria Staphylococcus aureus and Escherichia coli in vitro. For further application in vivo, low cytotoxicity and low acute toxicity in mice of ZnO were demonstrated. Finally, 4 nm ZnONPs combined with poly(vinyl alcohol) gel was used as antibacterial agent in rodent elytritis model, and significant anti-infection effect was proven. In one word, the present research would shed new light on the designing of antibacterial materials like ZnO with promising application in disinfection.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Center for Micro/nanomaterials and Technology, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Beijing 100190, P. R. China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Bai
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Huiyu
authors.initials: H
authors.lastname: Liu
authors.forename: Longfei
authors.initials: L
authors.lastname: Tan
authors.forename: Tianlong
authors.initials: T
authors.lastname: Liu
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D016585:Vaginosis, Bacterial / Q000188:drug therapy* / Q000382:microbiology; D015034:Zinc Oxide / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D015034:Zinc Oxide
keywords: ZnO nanoparticles; antibacterial activities; elytritis model; minimum inhibitory concentrations