title: [Research methods in protein-protein and protein-nucleic acid interactions and application in the study of human enterovirus A71].
abstract: Methods for analyses of protein-protein interactions include: yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include: yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
authors.affiliation: enter for Disease Control and Prevention, Chengdu Military Region, Kunming, China.
authors.forename: Zhi-Xiao
authors.initials: ZX
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zheng
authors.forename: Ting-Song
authors.initials: TS
authors.lastname: Hu
authors.forename: Qi-Han
authors.initials: QH
authors.lastname: Li
authors.forename: Quan-Shui
authors.initials: QS
authors.lastname: Fan
mesh_terms: D024202:Electrophoretic Mobility Shift Assay; D029821:Enterovirus A, Human / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D012367:RNA, Viral / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins