title: The inhibitory role of miR-214 in cervical cancer cells through directly targeting mitochondrial transcription factor A (TFAM).
abstract: Mitochondrial transcription factor A (TFAM) is a high-mobility group (HMG) protein and acts as a key regulator in mitochondrial DNA (mtDNA) replication, transcription, and inheritance. Accumulating evidence has demonstrated that TFAM plays an important role in tumorigenesis; however, the regulatory mechanism of TFAM in cervical cancer has not been revealed. In the current study, the au- thors found that with malignancy of cervical cancer, the protein expression of TFAM was gradually increased, while the expression of miRNA-214 was gradually downregulated. They further identified that TFAM is a target of miR-214. Forced overexpression of miRNA-214 significantly suppressed cell proliferation, cell cycle progression, colony-formation, and migration of cervical cancer Hela and Caski cells; however, upregulation of TFAM notably promoted cell proliferation, cell cycle progression, colony-formation, and migration of Hela and Caski cells. The authors further showed that miR-214 enhanced the susceptibility of Hela and Caski cells to the chemotherapy drug cisplatin. In conclusion, the current study provides a new sight for the regulatory pattern of miRNA-214 and TFAM in cervical cancer in vitro, indicating that miRNA-214 and MTFA may become important candidates for developing promising therapeutic strategies for the treatment of cervical cancer.
authors.affiliation: Department ofOncology, The Second Hospital of Central South University, Changsha, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Wen
authors.forename: Z
authors.initials: Z
authors.lastname: Lei
authors.forename: M
authors.initials: M
authors.lastname: Jin-An
authors.forename: L
authors.initials: L
authors.lastname: Xue-Zhen
authors.forename: Z
authors.initials: Z
authors.lastname: Xing-Nan
authors.forename: D
authors.initials: D
authors.lastname: Xiu-Wen
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology
chemical_list: D004268:DNA-Binding Proteins; C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D002945:Cisplatin