title: Fibrinogen alpha and beta gene polymorphisms in pediatric stroke--case-control and family based study.
abstract: OBJECTIVE
  Data on the role of the -455G > A polymorphism of the gene encoding β fibrinogen subunit (FGB) and the Thr312Ala polymorphism of the gene for the α fibrinogen subunit (FGA) in childhood ischemic stroke are insufficient. Therefore the aim of the study was to evaluate a possible association between these two polymorphisms and arterial ischemic stroke.
  
  
  METHODS
  The study group consisted of 85 children after ischemic stroke, 146 of their parents and 159 controls. Both polymorphisms were genotyped using the restriction fragment length polymorphism method. Two study designs were used: a case-control model and a family-based transmission-disequilibrium test. Statistica 7.1 and EpiInfo 6 softwares were used in all analyses.
  
  
  RESULTS
  In the TDT test, a tendency to a higher transmission of the 312Ala allele of the FGA gene and the -455A allele of the FGB gene was observed, however, it was statistically non-significant. The frequencies of alleles and genotypes of both FGA and FGB genes polymorphisms did not differentiate children from both groups also in the case-control model. Additive or synergistic effects between FGA and FGB genes polymorphisms were not observed.
  
  
  CONCLUSIONS
  An analysis of the results obtained in this study and a critical review of previously published data indicate that examined gene polymorphisms are not related to ischemic stroke in children.
authors.affiliation: School of Medicine in Katowice, Medical University of Silesia, Department of Paediatrics and Developmental Age Neurology, Chair of Paediatrics, Medykow Str 16, 40-752 Katowice, Poland. Electronic address: ilonakopyta@autograf.pl.
authors.forename: I
authors.initials: I
authors.lastname: Kopyta
authors.affiliation: School of Health Sciences in Katowice, Medical University of Silesia, Department of Biochemistry and Medical Genetics, Medykow Str 18, 40-752 Katowice, Poland. Electronic address: delog@op.pl.
authors.forename: P
authors.initials: P
authors.lastname: Niemiec
authors.affiliation: School of Health Sciences in Katowice, Medical University of Silesia, Department of Biochemistry and Medical Genetics, Medykow Str 18, 40-752 Katowice, Poland. Electronic address: abalcerzyk@poczta.onet.pl.
authors.forename: A
authors.initials: A
authors.lastname: Balcerzyk
authors.affiliation: School of Medicine in Katowice, Medical University of Silesia, Department of Paediatrics and Developmental Age Neurology, Chair of Paediatrics, Medykow Str 16, 40-752 Katowice, Poland. Electronic address: neurdziec@sum.edu.pl.
authors.forename: E
authors.initials: E
authors.lastname: Emich-Widera
authors.affiliation: Department of Developmental Neurology, Medical University of Gdansk, Debinki Str 7, 80-211 Gdansk, Poland. Electronic address: pilar@amg.gda.pl.
authors.forename: E
authors.initials: E
authors.lastname: Pilarska
authors.affiliation: Department of Developmental Neurology, Medical University of Gdansk, Debinki Str 7, 80-211 Gdansk, Poland. Electronic address: pienczk@gumed.edu.pl.
authors.forename: K
authors.initials: K
authors.lastname: Pienczk-Ręcławowicz
authors.affiliation: Department of Pediatric and Adolescent Neurology, Jagiellonian University Medical College, Wielicka Str 265, 30-663 Kraków, Poland. Electronic address: wgrandys@wp.pl.
authors.forename: M
authors.initials: M
authors.lastname: Kaciński
authors.affiliation: Department of Neurology, Polish Mother's Memorial Hospital-Research Institute, Rzgowska Str 281/289, 93-338 Łódź, Poland. Electronic address: janusz_wendorff@o2.pl.
authors.forename: J
authors.initials: J
authors.lastname: Wendorff
authors.affiliation: School of Health Sciences in Katowice, Medical University of Silesia, Department of Biochemistry and Medical Genetics, Medykow Str 18, 40-752 Katowice, Poland. Electronic address: biogen@sum.edu.pl.
authors.forename: T
authors.initials: T
authors.lastname: Nowak
authors.affiliation: School of Health Sciences in Katowice, Medical University of Silesia, Department of Biochemistry and Medical Genetics, Medykow Str 18, 40-752 Katowice, Poland. Electronic address: biogen@sum.edu.pl.
authors.forename: T
authors.initials: T
authors.lastname: Iwanicki
authors.affiliation: School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Drug Form Technology, Chair of Applied Pharmacy, Kasztanowa Str. 3, 41-205 Sosnowiec, Poland. Electronic address: bsarecka-hujar@sum.edu.pl.
authors.forename: B
authors.initials: B
authors.lastname: Sarecka-Hujar
authors.affiliation: School of Health Sciences in Katowice, Medical University of Silesia, Department of Biochemistry and Medical Genetics, Medykow Str 18, 40-752 Katowice, Poland. Electronic address: izak@sum.edu.pl.
authors.forename: I
authors.initials: I
authors.lastname: Zak
mesh_terms: D000293:Adolescent; D000483:Alleles; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005340:Fibrinogen / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D020521:Stroke / Q000235:genetics*
chemical_list: D005340:Fibrinogen
keywords: Children; FGA gene; FGB gene; Gene polymorphism; Stroke