title: Detection of Thyroid Papillary Carcinoma Lymph Node Metastases Using One Step Nucleic Acid Amplification (OSNA): Preliminary Results.
abstract: Purpouse: One Step Nucleic Acid Amplification (OSNA) has been previously proposed for the diagnosis of lymph node metastases (LNMs) from several malignant conditions by quantifying the number of copies of cytokeratin 19 mRNA. Our aim was to evaluate the results obtained by OSNA in the lymph nodes of patients with papillary thyroid carcinoma (PTC) by comparing our results with the findings observed using standard pathological examination.
  
  
  METHODS
  Fifty human lymph nodes (from five patients with diagnosed PTC) were studied. Each node was divided into two: one half was used for molecular study ("OSNA-node"), and the other half was used for conventional staining with hematoxylin and eosin ("HE-non-OSNA node"). Three cytological imprints using Papanicolaou and May-Grunwald-Giemsa strains were obtained from both node halves. The results from each technique were compared, and ROC analysis was performed.
  
  
  RESULTS
  The OSNA study showed 22 positive samples for LNM (44%), which demonstrate a high concordance rate with the results observed using conventional pathological examination (cytology of "OSNA-node" and HE of "Non-OSNA node") with specificity and sensitivity values greater than 86% and 89%, respectively. However, both comparisons differed in the number of copies of mRNA as the best cut-off (260 copies in the first case and 93 in the second case).
  
  
  CONCLUSIONS
  The OSNA results for the detection of LNM in patients with PTC are comparable with those observed using conventional techniques. However, its quantitative nature could be useful to more accurately detect lymph node involvement.
authors.affiliation: Department of General Surgery, Endocrine Surgery Division.
authors.forename: Oscar
authors.initials: O
authors.lastname: González
authors.forename: Carmela
authors.initials: C
authors.lastname: Iglesias
authors.forename: Carles
authors.initials: C
authors.lastname: Zafon
authors.forename: Josep
authors.initials: J
authors.lastname: Castellví
authors.forename: Amparo
authors.initials: A
authors.lastname: García-Burillo
authors.forename: Jordi
authors.initials: J
authors.lastname: Temprana
authors.forename: Enric
authors.initials: E
authors.lastname: Caubet
authors.forename: Ramón
authors.initials: R
authors.lastname: Vilallonga
authors.forename: Jordi
authors.initials: J
authors.lastname: Mesa
authors.forename: Santiago
authors.initials: S
authors.lastname: Ramón y Cajal
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Fort
authors.forename: Manel
authors.initials: M
authors.lastname: Armengol
authors.forename: José María
authors.initials: JM
authors.lastname: Balibrea
mesh_terms: D000328:Adult; D000368:Aged; D002277:Carcinoma / Q000473:pathology*; D002291:Carcinoma, Papillary; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000473:pathology*; D055815:Young Adult
keywords: OSNA; lymph node metastases; papillary thyroid carcinoma