title: Proteomic investigation of the severe preeclampsia treatment by low molecular weight heparin.
abstract: OBJECTIVE
  The primary goal of this study is to investigate the mechanism of severe preeclampsia (PE) treatment by low molecular weight heparin (LMWH).
  
  
  METHODS
  Using two-dimensional difference in-gel electrophoresis (2D-DIGE) combined with matrix assisted laser desorption ionization-time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF) approach to identify the proteins that expressed differently in the serum samples of five patients before and after subcutaneous injection of LMWH (0.4 ml/person).
  
  
  RESULTS
  Seven protein spots were identified in 2D-DIGE that show significant change in expression level after LMWH treat- ment. Further analysis of seven protein spots with MALDI-TOF/TOF identified six different proteins. To confirm the proteomic data, two meaningful proteins of the six proteins, alpha-1-acid glycoprotein (AGP) and serotransferrin are subjected to immunoblotting. All of the proteins are obviously down-regulated after LMWH treatment.
  
  
  CONCLUSIONS
  PE is a pregnancy-specific disease that clinically manifests as new-onset hypertension and proteinuria after 20 weeks of gestation. LMWH is an effective treatment of severe PE. The present proteomics based investigation may provide a new angle to understand the mechanism of severe PE treatment with LMWH.
authors.affiliation: The Department of Obstetrics and Gynecology, 2nd Medical College of Jinan University, Shenzhen People’s Hospital, China.
authors.forename: Shuoshi
authors.initials: S
authors.lastname: Wang
authors.forename: Shuiwang
authors.initials: S
authors.lastname: Hu
authors.forename: Mei
authors.initials: M
authors.lastname: Zhong
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006495:Heparin, Low-Molecular-Weight / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D009961:Orosomucoid / Q000032:analysis; D011225:Pre-Eclampsia / Q000188:drug therapy* / Q000378:metabolism; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014168:Transferrin / Q000032:analysis
chemical_list: D001798:Blood Proteins; D006495:Heparin, Low-Molecular-Weight; D009961:Orosomucoid; D014168:Transferrin