title: Use of a new enzyme extraction system to improve the sensitivity of SOS/umu test and application to environmental samples.
abstract: The purpose of this study was to find a better enzyme extraction reagent for the SOS/umu test to replace the conventional one (the combination of sodium dodecyl sulfate (SDS) and Z-buffer), which has the disadvantage of denaturing β-galactosidase leading to decreased measurement sensitivity. By adopting a microplate system, the performance of the umu test using BugBuster Master Mix, a commercially available enzyme extraction reagent, was compared with that using the conventional reagent for detecting the genotoxicity of known mutagens as well as environmental samples. BugBuster Master Mix was found to increase the detection sensitivities of the selected genotoxins and environmental water samples, due to the fact that it doesn't denature β-galactosidase. The result of this study showed that BugBuster Master Mix could be a better enzyme extraction reagent for umu test.
authors.affiliation: Key Laboratory of Drinking Water Science and Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing, 100085, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Tian
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Oda
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
mesh_terms: D004249:DNA Damage; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity*; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D009153:Mutagens; D014874:Water Pollutants, Chemical; D012967:Sodium Dodecyl Sulfate; C078202:UmuC protein, E coli; D004259:DNA-Directed DNA Polymerase; D001616:beta-Galactosidase