title: Determination of fatty acids and stable carbon isotopic ratio in subcutaneous fat to identify the feeding regime of Iberian pigs.
abstract: Discrimination among the types of feeding regimes for Iberian pigs is currently a highly demanded challenge by the Iberian pig sector. In the present research, discrimination among feeding regimes has been achieved by the combination of two analytical methods (based on FAMEs analysis by GC-FID and determination of δ(13)C by IRMS) previously used independently without success. In the present study, 80 samples of adipose tissue from Iberian pigs subjected to four different feedings were analyzed. The study of the variables more influenced by the feeding regime has allowed us to configure panels of markers with predictive power for the studied feedings by multivariate ROC analysis. The results provided values of specificity and sensitivity higher than 85% in most cases. The statistical combination of results from different analytical methods could be the key to develop models for the correct discrimination of Iberian pigs according to the feeding regime.
authors.affiliation: Department of Analytical Chemistry, University of Córdoba , Annex C-3, Campus of Rabanales, Córdoba, Spain.
authors.forename: María Asunción
authors.initials: MA
authors.lastname: López-Bascón
authors.forename: Feliciano
authors.initials: F
authors.lastname: Priego-Capote
authors.forename: Mónica
authors.initials: M
authors.lastname: Calderón-Santiago
authors.forename: Verónica
authors.initials: V
authors.lastname: Sánchez de Medina
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Moreno-Rojas
authors.forename: Juan María
authors.initials: JM
authors.lastname: García-Casco
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Luque de Castro
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002247:Carbon Isotopes / Q000737:chemistry / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D050151:Subcutaneous Fat / Q000737:chemistry* / Q000378:metabolism; D013552:Swine / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D005227:Fatty Acids
keywords: GC−FID; IRMS; Iberian pig; ROC analysis; fatty acid methyl esters; feeding regimes; stable carbon isotopes; subcutaneous fat