title: [Calcineurin subunit B is not an effective adjuvant when combined with a novel HBV protein particle vaccine].
abstract: To compare different adjuvant formulation and explore the impact of Calcineurin B subunit(CnB) as adjuvant with a novel HBV protein particle (HBSS1) vaccine in mice, female C57BL/6 mice were immunized HBSS1 with Al(OH)3 only, or a normal dose (5 μg) CnB only, or (CnB+ Al(OH)3) mixture as the adjuvant. All immunized groups were primed twice at 4-week intervals; followed by boosting with recombinant adenoviral based HBV vaccine(rAdSS1) at 10-week intervals. We detected the antigen specific humoral response in mice, including total IgG antibody and IgG subtyping. Then, we characterized the specific cell-mediated immune (CMI) response by detection of γ-interferon secreting splenocytes after stimulaton with S or PreS1 peptide pools. No enhancement of immunity was found among the mice with 5 μg of CnB alone or combined with Al(OH), adjuvanted vaccine,which could not induce higher level of anti-PreS1 and anti-S antibodies and CMI than that of HBSS1 alone or Al(OH)3 adjuvanted vaccines. We concluded that CnB is not an effective adjuvant for a novel HBV subunit vaccine.
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chuai
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.forename: Yao
authors.initials: Y
authors.lastname: Deng
authors.forename: Li
authors.initials: L
authors.lastname: Ruan
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Tan
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D019703:Calcineurin / Q000494:pharmacology*; D005260:Female; D017325:Hepatitis B Vaccines / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D021122:Protein Subunits
chemical_list: D000276:Adjuvants, Immunologic; D017325:Hepatitis B Vaccines; D021122:Protein Subunits; D019703:Calcineurin