title: Characterization of the murine myeloid precursor cell line MuMac-E8.
abstract: Starting point for the present work was the assumption that the cell line MuMac-E8 represents a murine cell population with stem cell properties. Preliminary studies already pointed to the expression of stem-cell associated markers and a self-regenerative potential of the cells. The cell line MuMac-E8 should be examined for their differential stage within stem cell hierarchy. MuMac-E8 cells were derived from a chimeric mouse model of arthritis. It could be shown that MuMac-E8 cells express mRNA of some genes associated with pluripotent stem cells (Nanog, Nucleostemin), of genes for hematopoietic markers (EPCR, Sca-1, CD11b, CD45), for the mesenchymal marker CD105 and of genes for the neural markers Pax-6 and Ezrin. In methylcellulose and May-Grünwald-Giemsa staining, hematopoietic colonies were obtained but the hematopoietic system of lethally irradiated mice could not be rescued. Osteogenic differentiation was not detectable. Thus, it became evident that MuMac-E8 represents not a stem cell line. However, MuMac-E8 cells expressed several myeloid surface markers (i.e. CD11b, F4/80, CD14, CD64), showed phagocytosis and is capable of producing nitric oxide. Thus, this cell line seems to be arrested an advanced stage of myeloid differentiation. Adherence data measured by impedance-based real-time cell analysis together with cell morphology data suggested that MuMac-E8 represents a new macrophage precursor cell line exhibiting weak adherence. This cell line is suitable as an in-vitro model for testing of macrophage functions. Moreover, it might be also useful for differentiation or reprogramming studies.
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany; Institute of Clinical Immunology, University of Leipzig, Leipzig, Germany; Translational Centre for Regenerative Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Fricke
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Cathleen
authors.initials: C
authors.lastname: Pfefferkorn
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany; Department of Surgery, Research Laboratories, University of Leipzig, Leipzig, Germany.
authors.forename: Doris
authors.initials: D
authors.lastname: Wolf
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Sina
authors.initials: S
authors.lastname: Riemschneider
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Janine
authors.initials: J
authors.lastname: Kohlschmidt
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Nadja
authors.initials: N
authors.lastname: Hilger
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Fueldner
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Knauer
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany; Institute of Clinical Immunology, University of Leipzig, Leipzig, Germany; Translational Centre for Regenerative Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Sack
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany; Institute of Clinical Immunology, University of Leipzig, Leipzig, Germany; Translational Centre for Regenerative Medicine, University of Leipzig, Leipzig, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Emmrich
authors.affiliation: Fraunhofer Institute for Cell Therapy and Immunology (IZI), Leipzig, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D006410:Hematopoiesis; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D010012:Osteogenesis; D013234:Stem Cells / Q000166:cytology* / Q000502:physiology