title: Cinnamate of inulin as a vehicle for delivery of colonic drugs.
abstract: Colon diseases are difficult to treat because oral administrated drugs are absorbed at the stomach and intestine levels and they do not reach colon; in addition, intravenous administrated drugs are eliminated from the body before reaching colon. Inulin is a naturally occurring polysaccharide found in many plants. It consists of β 2-1 linked D-fructose molecules having a glucosyl unit at the reducing end. Various inulin and dextran hydrogels have been developed that serve as potential carrier for introduction of drugs into the colon. Because inulin is not absorbed in the stomach or in the small intestine, and inulin is degraded by colonic bacteria, drugs encapsulated in inulin-coated vesicles could be specifically liberated in the colon. Therefore, the use of inulin-coated vesicles could represent an advance for the treatment of colon diseases. Here, we study the use of a cinnamoylated derivative of chicory inulin as a vehicle for the controlled delivery of colonic drugs. The encapsulation of methotrexate in inulin vesicles and its release and activity was studied in colon cancer cells in cultures.
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Dorotea
authors.initials: D
authors.lastname: López-Molina
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Chazarra
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Chee Wun
authors.initials: CW
authors.lastname: How
authors.affiliation: Durmishidze Institute of Biochemistry and Biotechnology of Agrarian University of Georgia, 0131 Tbilisi, Georgia.
authors.forename: Nikolov
authors.initials: N
authors.lastname: Pruidze
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Enma
authors.initials: E
authors.lastname: Navarro-Perán
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: García-Cánovas
authors.affiliation: Grupo de Química de Carbohidratos, Polímeros y Aditivos Industriales, Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Pedro Antonio
authors.initials: PA
authors.lastname: García-Ruiz
authors.affiliation: Grupo de Química de Carbohidratos, Polímeros y Aditivos Industriales, Departamento de Química Orgánica, Facultad de Química, Universidad de Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rojas-Melgarejo
authors.affiliation: Departamento de Bioquímica y Biología Molecular A, Facultad de Biología, Universidad de Murcia, 30100 Espinardo, Murcia, Spain. Electronic address: neptuno@um.es.
authors.forename: José Neptuno
authors.initials: JN
authors.lastname: Rodríguez-López
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D018651:Cichorium intybus; D002934:Cinnamates / Q000737:chemistry*; D003106:Colon / Q000378:metabolism*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006026:Glycoside Hydrolases / Q000737:chemistry; D006801:Humans; D007444:Inulin / Q000008:administration & dosage* / Q000737:chemistry*; D008727:Methotrexate / Q000008:administration & dosage* / Q000737:chemistry; D008863:Microspheres
chemical_list: D002934:Cinnamates; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase; D008727:Methotrexate
keywords: Cinnamoylated inulin; Colon drug delivery; Inulin; Inulinase; Methotrexate