title: Intracerebral antioxidant ability of mature rats after neonatal hypoxic-ischemic brain injury estimated using the microdialysis-electron spin resonance method.
abstract: OBJECTIVE
  The intracerebral antioxidant ability of mature rats after neonatal hypoxic-ischemic (HI) brain injury was estimated using the microdialysis-electron spin resonance method.
  
  
  METHODS
  Seven-day-old Wistar rats were subjected to a modified Levine's procedure for producing HI brain injury. After HI insult, pups were returned and reared with their dams. Seven weeks after HI insult, their intracerebral antioxidant abilities were measured using the microdialysis-electron spin resonance method after the intraperitoneal injection of 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl. Ascorbic acid, L-cysteine, and glutathione (GSH) were also determined. The rats without HI insult were used as a control.
  
  
  RESULTS
  The decay rate of 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in the non-ligated side of the cerebral hemisphere of the HI group was significantly larger than that of the control group. The amounts of ascorbic acid in the perfusate from the non-ligated side of the HI group were about four times larger than those of the control group. The amounts of L-cysteine and GSH of the HI group were about 10 times larger than those of the control group.
  
  
  CONCLUSIONS
  The antioxidant ability in the non-ligated sides of the cerebral hemispheres of the mature rats 7 weeks after neonatal HI insult was higher than that of the control group. Higher amounts of ascorbic acid and GSH supported the higher antioxidant ability. The increase of the intracerebral antioxidant ability of the non-ligated side indicates the compensation of motor function for the lost side. The present results should offer important insights into the prognosis for hypoxic-ischemic encephalopathy.
authors.affiliation: Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Nakajima
authors.affiliation: Section of Psychiatry, Department of Clinical Neuroscience, University of Miyazaki, Miyazaki, Japan.
authors.forename: Yuto
authors.initials: Y
authors.lastname: Ueda
authors.affiliation: Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sameshima
authors.affiliation: Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
authors.forename: Tsuyomu
authors.initials: T
authors.lastname: Ikenoue
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001205:Ascorbic Acid / Q000097:blood / Q000378:metabolism; D001720:Birth Injuries / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D002540:Cerebral Cortex / Q000098:blood supply / Q000293:injuries / Q000378:metabolism*; D003497:Cyclic N-Oxides / Q000032:analysis; D003545:Cysteine / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D004578:Electron Spin Resonance Spectroscopy; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology; D007231:Infant, Newborn; D017551:Microdialysis; D009474:Neurons / Q000378:metabolism*; D018384:Oxidative Stress; D011759:Pyrrolidines / Q000032:analysis; D017208:Rats, Wistar; D013113:Spin Labels
chemical_list: D000975:Antioxidants; D003497:Cyclic N-Oxides; C109806:MCPROXYL; D011759:Pyrrolidines; D013113:Spin Labels; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid
keywords: antioxidant ability; ascorbic acid; glutathione; hypoxia-ischemia; microdialysis-electron spin resonance method; neonatal rat