title: Complement component C5 recruits neutrophils in the absence of C3 during respiratory infection with modified vaccinia virus Ankara.
abstract: Efficient leukocyte migration is important for an effective host response to viral infection and the development of adaptive immunity. The poxvirus strain modified vaccinia virus Ankara (MVA), a safe and efficient viral vector, rapidly induces chemokine expression and respiratory recruitment of leukocytes, which is unique among vaccinia viruses. In addition to chemokines, the complement system contributes to the attraction and activation of different types of leukocytes. Using a murine model of intranasal infection, we show in this study that MVA-induced neutrophil recruitment depends on complement component C5. Remarkably, we find that C5 mediates neutrophil recruitment to the lung, even in the absence of the central complement component C3. Our findings argue for complement C5 activation during MVA infection of the lung via a C3-independent pathway, which enables rapid recruitment of neutrophils.
authors.affiliation: Institut für Infektionsmedizin und Zoonosen, Ludwig-Maximilians-Universität München, Munich 80539, Germany; German Centre for Infection Research, Partner Site Munich, Munich 80539, Germany;
authors.forename: Philip J R
authors.initials: PJ
authors.lastname: Price
authors.affiliation: Division of Virology, Medical University of Innsbruck, Innsbruck 6020, Austria;
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Bánki
authors.affiliation: Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, Abteilung für Vergleichende Medizin, Neuherberg 85764, Germany; and.
authors.forename: Angelika
authors.initials: A
authors.lastname: Scheideler
authors.affiliation: Division of Virology, Medical University of Innsbruck, Innsbruck 6020, Austria;
authors.forename: Heribert
authors.initials: H
authors.lastname: Stoiber
authors.affiliation: Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich 81675, Germany.
authors.forename: Admar
authors.initials: A
authors.lastname: Verschoor
authors.affiliation: Institut für Infektionsmedizin und Zoonosen, Ludwig-Maximilians-Universität München, Munich 80539, Germany; German Centre for Infection Research, Partner Site Munich, Munich 80539, Germany;
authors.forename: Gerd
authors.initials: G
authors.lastname: Sutter
authors.affiliation: Institut für Infektionsmedizin und Zoonosen, Ludwig-Maximilians-Universität München, Munich 80539, Germany; German Centre for Infection Research, Partner Site Munich, Munich 80539, Germany; Michael.Lehmann@lmu.de.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003176:Complement C3 / Q000235:genetics / Q000276:immunology; D003182:Complement C5 / Q000276:immunology*; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D020556:Neutrophil Infiltration / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D003176:Complement C3; D003182:Complement C5