title: Serum CD166: a novel hepatocellular carcinoma tumor marker.
abstract: BACKGROUND
  We evaluated the diagnostic value of serum-CD166 in patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  Tissue-CD166 was measured using immunohistochemistry. Cell proliferation and migration were evaluated using MTT and Transwell assays, respectively. Serum-CD166 was examined using ELISA and western blotting.
  
  
  RESULTS
  CD166 was up-regulated in HCC compared to those in normal liver tissues. Cell proliferation was positively correlated and cell migration was negatively correlated with endogenous CD166 expression in HCC cells. CD166 inhibition using specific shRNA decreased cell proliferation but increased cell migration. Serum CD166 concentrations were much higher in HCC than in colon cancer, hepatitis B, hepatitis C, cirrhosis, gastric cancer, breast cancer, lung cancer and healthy individuals. Serum CD166 also decreased dramatically after curative surgery. A positive correlation was found between serum CD166 and AFP (R=0.7141, p=0.000). Serum CD166 was also positively correlated with γ-GT, bile acid, ALT, AST, and ALP but was negatively correlated with Alb and pre-Alb. The area under the receiver operating characteristic curve for serum-CD166 was 0.9860, which was better than AFP (AUC-ROC, 0.9354) for the differentiation of HCC patients from healthy individuals, with a cut-off of 261 ng/ml (sensitivity: 100.00%, specificity: 89.41%).
  
  
  CONCLUSIONS
  Serum CD166 is a novel diagnostic tumor marker for HCC.
authors.affiliation: Department of Clinical laboratory medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Clinical laboratory medicine, Ruijin Hospital of Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Jiafei
authors.initials: J
authors.lastname: Lin
authors.affiliation: School of Public Health, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Clinical laboratory medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Weng
authors.affiliation: Department of Clinical laboratory medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Clinical laboratory medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China. Electronic address: karajan2@163.com.
authors.forename: Jiayi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical laboratory medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai 200072, China. Electronic address: sunfenyong@126.com.
authors.forename: Fenyong
authors.initials: F
authors.lastname: Sun
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000097:blood* / Q000378:metabolism; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D015816:Cell Adhesion Molecules, Neuronal / Q000097:blood* / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005326:Fetal Proteins / Q000097:blood* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D014408:Biomarkers, Tumor; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule
keywords: AFP; CD166; Liver cancer; Liver function test; Tumor marker; YAP