title: Effect of inulin on efficient production and regulatory biosynthesis of bacillomycin D in Bacillus subtilis fmbJ.
abstract: The effect of inulin on the production of bacillomycin D and the levels of mRNA of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC), the thioesterase gene (TE) and regulating genes: AbrB, ComA, DegU, PhrC, SigmaH and Spo0A in Bacillus subtilis fmbJ were investigated. The production of bacillomycin D was enhanced with the increase of biomass concentration. The maximum production and productivity of bacillomycin D were found to be 1227.49 mg/L and 10.23 mg/L h. Inulin significantly improved the expression of bacillomycin D synthetase genes: bmyA (BYA), bmyB (BYB), bmyC (BYC) and the thioesterase gene (TE). Also, inulin up-regulated ComA, DegU, SigmaH and Spo0A and therefore promoted the high production of bacillomycin D. Our results provided a practical approach for efficient production of bacillomycin D and a meaningful explanation for regulatory mechanism of bacillomycin D biosynthesis.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China; Department of Bioengineering and Food, Bengbu University, Bengbu 233030, China.
authors.forename: Shiquan
authors.initials: S
authors.lastname: Qian
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
authors.forename: Hedong
authors.initials: H
authors.lastname: Lu
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Meng
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China. Electronic address: lufengxia@njau.edu.cn.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Lv
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Bie
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, China. Electronic address: fmb@njau.edu.cn.
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D007444:Inulin / Q000494:pharmacology*; D010455:Peptides / Q000378:metabolism*; D013997:Time Factors
chemical_list: D023181:Antimicrobial Cationic Peptides; D010455:Peptides; C027845:bacillomycin D; D000255:Adenosine Triphosphate; D007444:Inulin
keywords: Bacillomycin D production; Fed-batch fermentation; Gene expression; Inulin; Regulatory mechanism