title: Anti-inflammatory effects of kolaviron modulate the expressions of inflammatory marker genes, inhibit transcription factors ERK1/2, p-JNK, NF-κB, and activate Akt expressions in the 93RS2 Sertoli cell lines.
abstract: The anti-inflammatory effects of kolaviron (Kol-v) have been demonstrated in several experimental models. The ability of Kol-v to modulate the expressions of inflammatory genes in lipopolysaccharide (LPS)-stimulated Sertoli cell line, 93RS2 was investigated in this study. Kol-v decreased the expressions of inflammatory genes TNF-α, Tlr-4, and Nfκb1 and has synergistic effect on LPS-induced COX-2 and iNOS expressions at high concentrations (25-100 μM). At lower concentrations (5-15 µM), the expressions of TNF-α, IL-6, and IL-1α were down-regulated by Kol-v except Tgfβ1 that was up-regulated. The LPS-induced decrease in the expression of the anti-inflammatory genes IL-3, IL-4, and IL-10 was blocked by Kol-v at all concentrations of Kol-v tested. The LPS-induced phosphorylations of mitogen-activated protein kinase family members (ERK1/2, and p-JNK), decreased IκBα expression, and decreased Akt phosphorylation was blocked by Kol-v. Our results highlight the potential for Kol-v at lower concentration to ameliorate cellular damage caused by local inflammation.
authors.affiliation: Department of Biochemistry, University of Port Harcourt, East-West Road, Choba, Nigeria, abarikwus@yahoo.com.
authors.forename: Sunny O
authors.initials: SO
authors.lastname: Abarikwu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D008070:Lipopolysaccharides; D014157:Transcription Factors; D051057:Proto-Oncogene Proteins c-akt; C045492:kolaviron