title: Lack of association between human herpesvirus and vestibular schwannoma: analysis of 121 cases.
abstract: OBJECTIVE
  To assess for the presence of human herpesvirus (HHV) using immunohistochemical and polymerase chain reaction (PCR) assay in surgically excised vestibular schwannoma (VS) samples.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  A retrospective laboratory-based study of tumors from patients with vestibular schwannoma.
  
  
  METHODS
  Tissue microarrays (TMAs) representing sporadic and NF2-associated VS from 121 patients, as well as appropriate positive and negative controls, were studied. TMA sections were immunostained using antibodies directed against HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, and HHV-8. PCR was used for the detection of all 8 known human herpesviruses.
  
  
  RESULTS
  There was no detectable HHV (HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, HHV-8) by immunohistochemistry in any of the 121 cases of sporadic and NF2 cases analyzed. These data were further validated by DNA sequence analyses using PCR in a subset of the VS samples, all of which were found to be negative for all HHV.
  
  
  CONCLUSIONS
  The data offer no support for an association between HHV and the development of sporadic or NF2-associated VS in humans.
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada.
authors.forename: Sanjiv K
authors.initials: SK
authors.lastname: Bhimrao
authors.affiliation: Department of Pathology and Laboratory Medicine, Division of Neuropathology, University of British Columbia, Vancouver, Canada.
authors.forename: John
authors.initials: J
authors.lastname: Maguire
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada.
authors.forename: Cathie
authors.initials: C
authors.lastname: Garnis
authors.affiliation: Department of Pathology and Laboratory Medicine, British Columbia Centre for Disease Control, University of British Columbia, Vancouver, Canada.
authors.forename: Patrick
authors.initials: P
authors.lastname: Tang
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada.
authors.forename: Jane
authors.initials: J
authors.lastname: Lea
authors.affiliation: Division of Neurosurgery, University of British Columbia, Vancouver, Canada.
authors.forename: Ryojo
authors.initials: R
authors.lastname: Akagami
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of British Columbia, Vancouver, Canada BWesterberg@providencehealth.bc.ca.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Westerberg
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009464:Neuroma, Acoustic / Q000175:diagnosis / Q000601:surgery / Q000821:virology*; D013506:Otologic Surgical Procedures / Q000379:methods*; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral
keywords: Epstein-Barr virus; acoustic neuroma; cytomegalovirus; etiology; herpes simplex virus; human herpesvirus; immunohistochemistry; varicella-zoster virus; vestibular schwannoma