title: Protein adsorption on electrospun zinc doped hydroxyapatite containing nylon 6 membrane: kinetics and isotherm.
abstract: Surface modification of electrospun polymeric membrane surfaces is a critical step towards the separation process including protein adsorption. In this study, the electrospun Nylon fibers was incorporated with positively charged zinc doped hydroxyapatite (HAp) nanoparticles to study the adsorption of negatively charged proteins, namely bovine serum albumin (BSA). Effects of zinc amount within the atomic structure of HAp (nZH; n=0, 4, 8 At.%) was evaluated on produced scaffolds and consequently protein adsorption. The results showed that the ability of Nylon membrane to adsorb BSA increased with incorporation of nZH nanoparticles within the nylon structure. This phenomenon is appeared to be relate to different electrostatic charge and not to physical characteristic of scaffolds. The incorporated membrane (N-4ZH) by nanoparticles with highest zeta (ξ) potential adsorbed the maximum amount of protein. The adsorption of BSA was best fitted with pseudo-second order kinetic model. The experimental isotherm data were further analyzed by using Langmuir and Freundlich equations. By comparing the correlation coefficients obtained for each linear transformation of isotherm analysis, it was found that the Langmuir equation was the best fit equilibrium model that described the adsorption of BSA on these membranes.
authors.affiliation: Materials and Energy Research Center (MERC), Karaj, Iran; START - Thrust 3, Create Research Wing, #03-08, 1 Create Way, National University of Singapore, Singapore 138602, Singapore.
authors.forename: Hamid
authors.initials: H
authors.lastname: Esfahani
authors.affiliation: START - Thrust 3, Create Research Wing, #03-08, 1 Create Way, National University of Singapore, Singapore 138602, Singapore. Electronic address: nnimpp@nus.edu.sg.
authors.forename: Molamma P
authors.initials: MP
authors.lastname: Prabhakaran
authors.affiliation: Materials and Energy Research Center (MERC), Karaj, Iran. Electronic address: e-salahi@merc.ac.ir.
authors.forename: Esmaeil
authors.initials: E
authors.lastname: Salahi
authors.affiliation: Materials and Energy Research Center (MERC), Karaj, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Tayebifard
authors.affiliation: Materials and Energy Research Center (MERC), Karaj, Iran.
authors.forename: Mansour
authors.initials: M
authors.lastname: Keyanpour-Rad
authors.affiliation: Materials and Energy Research Center (MERC), Karaj, Iran.
authors.forename: Mohamad Reza
authors.initials: MR
authors.lastname: Rahimipour
authors.affiliation: START - Thrust 3, Create Research Wing, #03-08, 1 Create Way, National University of Singapore, Singapore 138602, Singapore; Department of Mechanical Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
mesh_terms: D000327:Adsorption; D000818:Animals; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism*; D004595:Electroplating / Q000379:methods*; D007700:Kinetics; D008567:Membranes, Artificial; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D015032:Zinc / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; C009916:nylon 6; D012710:Serum Albumin, Bovine; D002209:Caprolactam; D017886:Durapatite; D015032:Zinc
keywords: Apatite; Electrospinning; Fibers; Membranes