title: Recombinant transferrin binding protein A (rTbpA) fragments of Pasteurella multocida serogroup B:2 provide variable protection following homologous challenge in mouse model.
abstract: Transferrin binding protein A (TbpA), an iron acquisition surface protein that also acts as virulence factor, is widely distributed among strains of Pasteurella multocida. In the present study, a total of seven clones of TbpA fragments (39D to F777; 39D to Q697; 188V to F777; 188V to Q697; 39D to P377; 188V to P377 and 39D to F187) belonging to P. multocida B:2 were constructed, over-expressed and purified as recombinant fusion proteins from Escherichia coli using affinity chromatography. Immunization of mice with rTbpA fragments resulted in a significant (p < 0.05) rise in antigen specific serum total IgG and subtypes (IgG1 and IgG2a) tires. All immunized mice challenged with 8 LD50 of P. multocida B:2 resulted in a variable protective efficacy up to 50%. The study indicated the potential possibilities to incorporate full length TbpA in subunit vaccine formulation composed of synergistic subunit antigens against haemorrhagic septicaemia (HS) in cattle and buffalo.
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital, Uttarakhand, India. Electronic address: sbshivachandra@gmail.com.
authors.forename: Sathish Bhadravati
authors.initials: SB
authors.lastname: Shivachandra
authors.affiliation: National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Bengaluru-560024, Karnataka, India.
authors.forename: Revanaiah
authors.initials: R
authors.lastname: Yogisharadhya
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital, Uttarakhand, India.
authors.forename: Abhinendra
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Clinical Bacteriology Laboratory, Indian Veterinary Research Institute (IVRI), Mukteswar-263138, Nainital, Uttarakhand, India.
authors.forename: Nihar Nalini
authors.initials: NN
authors.lastname: Mohanty
authors.affiliation: Division of Bacteriology and Mycology, Indian Veterinary Research Institute (IVRI), Izatnagar-243122, Bareilly, Uttar Pradesh, India.
authors.forename: Viswas Konasagara
authors.initials: VK
authors.lastname: Nagaleekar
mesh_terms: D000820:Animal Diseases / Q000276:immunology / Q000517:prevention & control*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001428:Bacterial Vaccines / Q000276:immunology / Q000627:therapeutic use*; D002020:Buffaloes; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control*; D004195:Disease Models, Animal; D004926:Escherichia coli; D006483:Hemorrhagic Septicemia / Q000517:prevention & control / Q000662:veterinary*; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D016979:Pasteurella multocida / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology / Q000627:therapeutic use; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000627:therapeutic use*; D065288:Serogroup; D033881:Transferrin-Binding Protein A / Q000276:immunology / Q000627:therapeutic use*
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D033881:Transferrin-Binding Protein A
keywords: Immunogenicity; Mouse model; Pasteurella multocida; Protective efficacy; Recombinant transferrin binding protein A (rTbpA)