title: Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: native ribonucleoprotein immunoprecipitation (RIP) assays.
abstract: Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis-acting regulatory regions in the mRNA transcript that mediate direct interactions with trans-acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.
authors.affiliation: Department of Surgery, The Jefferson Pancreas, Biliary, and Related Cancer Center, Thomas Jefferson University, 623 College Building, 1015 Walnut St., Philadelphia, PA, 19107, USA.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Cozzitorto
authors.forename: Masaya
authors.initials: M
authors.lastname: Jimbo
authors.forename: Saswati
authors.initials: S
authors.lastname: Chand
authors.forename: Fernando
authors.initials: F
authors.lastname: Blanco
authors.forename: Shruti
authors.initials: S
authors.lastname: Lal
authors.forename: Melissa
authors.initials: M
authors.lastname: Gilbert
authors.forename: Jordan M
authors.initials: JM
authors.lastname: Winter
authors.forename: Myriam
authors.initials: M
authors.lastname: Gorospe
authors.forename: Jonathan R
authors.initials: JR
authors.lastname: Brody
mesh_terms: D000818:Animals; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012261:Ribonucleoproteins