title: Separation of tubulin and microtubule-associated proteins by ion exchange chromatography.
abstract: Conventional liquid chromatography on phosphocellulose (PC) can be used to separate tubulin and microtubule-associated proteins (MAPs). Tubulin is a highly acidic protein and thus does not bind to PC. MAPs, however, do bind to PC and can be eluted with a subsequent salt wash of the column.
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire 03755.
authors.forename: Roger D
authors.initials: RD
authors.lastname: Sloboda
mesh_terms: D000818:Animals; D002852:Chromatography, Ion Exchange; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D014404:Tubulin / Q000302:isolation & purification*
chemical_list: D008869:Microtubule-Associated Proteins; D014404:Tubulin