title: [Neural stem cell-specific peroxisome proliferator-activated receptor γ knockout mice: breeding and genetic identification].
abstract: OBJECTIVE
  To breed neual stem cell-specific peroxisome proliferator-activated receptor γ (PPARγ) knockout mice.
  
  
  METHODS
  Two transgenic mouse models, namely B6.PPARγloxp/loxp and B6.Nestin-Cre were interbred, and the first- generation offsprings were backcrossed with B6.PPARγloxp/loxp to obtain the second-generation mice. Genomic DNA was extracted from the second-generation mice for PCR to amplify the loxp and Cre gene fragments followed by agarose gel electrophoresis to verify their sizes. The mice with the PPARγloxp/loxp.Nestin-Cre (KO) genotype were selected as the neural stem cell-specific knockout PPARγ mice, with B6.PPARγloxp/loxp (loxp) mice as the control. Tissue samples were collected from specific regions of the mouse brain and peripheral tissue for detecting the expression of PPARγ mRNA using RT-PCR and real-time quantitative PCR.
  
  
  CONCLUSIONS
  Genotyping results showed PPARγloxp and Cre bands in the knockout mice, which showed obviously decreased mRNA expression of PPARγ, suggesting successful establishment of neural stem cell-specific PPARγ knockout mice. The two transgenic mice we used were fertile, and their breeding pattern followed the laws of Mendelian inheritance.
authors.affiliation: Department of Neurobiology, Southern Medical University, Guangzhou 510515, China.E-mail: wuqiaoqi1209@126.com.
authors.forename: Qiaoqi
authors.initials: Q
authors.lastname: Wu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Lifang
authors.initials: L
authors.lastname: Lin
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D005838:Genotype; D051379:Mice; D018345:Mice, Knockout; D058953:Neural Stem Cells; D047495:PPAR gamma / Q000235:genetics*; D012333:RNA, Messenger
chemical_list: D047495:PPAR gamma; D012333:RNA, Messenger