title: Global multilocus sequence typing analysis of Mycoplasma bovis isolates reveals two main population clusters.
abstract: Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better understanding of the genetic diversity of this pathogen, a multilocus sequence typing (MLST) scheme was developed and applied to the characterization of 137 M. bovis isolates from diverse geographical origins, obtained from healthy or clinically infected cattle. After in silico analysis, a final set of 7 housekeeping genes was selected (dnaA, metS, recA, tufA, atpA, rpoD, and tkt). MLST analysis demonstrated the presence of 35 different sequence types (STs) distributed in two main clonal complexes (CCs), defined at the double-locus variant level, namely, CC1, which included most of the British and German isolates, and CC2, which was a more heterogeneous and geographically distant group of isolates, including European, Asian, and Australian samples. Index of association analysis confirmed the clonal nature of the investigated M. bovis population, based on MLST data. This scheme has demonstrated high discriminatory power, with the analysis showing the presence of genetically distant and divergent clusters of isolates predominantly associated with geographical origins.
authors.affiliation: Animal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom ruben.rosales@apha.gsi.gov.uk.
authors.forename: R S
authors.initials: RS
authors.lastname: Rosales
authors.affiliation: Animal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
authors.forename: C P
authors.initials: CP
authors.lastname: Churchward
authors.affiliation: Institute for Molecular Pathogenesis, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Schnee
authors.affiliation: Institute for Molecular Pathogenesis, Friedrich Loeffler Institute, Federal Research Institute for Animal Health, Jena, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Sachse
authors.affiliation: Kimron Veterinary Institute, Beit Dagan, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Lysnyansky
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Catania
authors.affiliation: Istituto Zooprofilattico Sperimentale delle Venezie, Legnaro, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Iob
authors.affiliation: Animal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
authors.forename: R D
authors.initials: RD
authors.lastname: Ayling
authors.affiliation: Animal and Plant Health Agency-Weybridge, Addlestone, Surrey, United Kingdom.
authors.forename: R A J
authors.initials: RA
authors.lastname: Nicholas
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D016000:Cluster Analysis; D005798:Genes, Bacterial; D020043:Genes, Essential; D014644:Genetic Variation; D005838:Genotype; D014943:Global Health; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D045682:Mycoplasma bovis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D058974:Phylogeography