title: Ethanol extract of Dalbergia odorifera protects skin keratinocytes against ultraviolet B-induced photoaging by suppressing production of reactive oxygen species.
abstract: Dalbergia odorifera T. Chen (Leguminosae), an indigenous medicinal herb, has been widely used in northern and eastern Asia to treat diverse diseases. Here, we investigated the anti-senescent effects of ethanolic extracts of Dalbergia odorifera (EEDO) in ultraviolet (UV) B-irradiated skin cells. EEDO significantly inhibited UVB-induced senescence of human keratinocytes in a concentration-dependent manner, concomitant with inhibition of reactive oxygen species (ROS) generation. UVB-induced increases in the levels of p53 and p21, biomarkers of cellular senescence, were almost completely abolished in the presence of EEDO. Sativanone, a major constituent of EEDO, also attenuated UVB-induced senescence and ROS generation in keratinocytes, indicating that sativanone is an indexing (marker) molecule for the anti-senescence properties of EEDO. Finally, treatment of EEDO to mice exposed to UVB significantly reduced ROS levels and the number of senescent cells in the skin. Thus, EEDO confers resistance to UVB-induced cellular senescence by inhibiting ROS generation in skin cells.
authors.affiliation: a Department of Animal Biotechnology , Konkuk University , Seoul , Republic of Korea.
authors.forename: Sun Ah
authors.initials: SA
authors.lastname: Ham
authors.forename: Jung Seok
authors.initials: JS
authors.lastname: Hwang
authors.forename: Eun Sil
authors.initials: ES
authors.lastname: Kang
authors.forename: Taesik
authors.initials: T
authors.lastname: Yoo
authors.forename: Hyun Ho
authors.initials: HH
authors.lastname: Lim
authors.forename: Won Jin
authors.initials: WJ
authors.lastname: Lee
authors.forename: Kyung Shin
authors.initials: KS
authors.lastname: Paek
authors.forename: Han Geuk
authors.initials: HG
authors.lastname: Seo
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D002648:Child; D002851:Chromatography, High Pressure Liquid; D035782:Dalbergia / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D008812:Mice, Hairless; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology; D017382:Reactive Oxygen Species; D012867:Skin / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D013473:Sunscreening Agents / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects
chemical_list: D007529:Isoflavones; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; C000607319:sativanone; D000431:Ethanol
keywords: Dalbergia odorifera; reactive oxygen species; sativanone; senescence; ultraviolet