title: [BRAF V600E mutation detection in melanoma patients: clinical and morphological features].
abstract: OBJECTIVE
  To estimate the frequency of BRAF V600E mutation in melanoma patients and to assess an association of this type of mutation with the clinical and morphological characteristics of the disease.
  
  
  METHODS
  The mutation of BRAF V600E was analyzed using an allele-specific real-time PCR assay in 71 melanoma patients treated at the Krasnoyarsk Territorial Oncology Dispensary. Before DNA isolation, the glasses containing the material obtained from the patients and stained with hematoxylin and eosin were tested for the content of tumor cells in the sections. Later on, genomic DNA was isolated with a FFPET DNA-Extraction kit (Biolink, Russia) and the real-time PCR assay was carried out.
  
  
  RESULTS
  The BRAF V600E mutation was observed in 40.84% of the patients and was not in 59.16%. There were specific features in tumor location and histotype in relation to the status of BRAF.
  
  
  CONCLUSIONS
  To detect whether the V600E mutation is present or absent in the BRAF gene in melanoma patients is an important component in the evaluation of the biological behavior of tumor cells and their performance of biologically significant functions, such as tumor invasion and dissemination and neoangiogenesis, which is important for the further assessment of tumor progression and disease prognosis.
authors.forename: M V
authors.initials: MV
authors.lastname: Aksenenko
authors.forename: S S
authors.initials: SS
authors.lastname: Bekuzarov
authors.forename: T G
authors.initials: TG
authors.lastname: Ruksha
mesh_terms: D000368:Aged; D000483:Alleles; D005260:Female; D006801:Humans; D008297:Male; D008545:Melanoma / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D017354:Point Mutation; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D012426:Russia
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf