title: Efficacy of Zhuling polyporus polysaccharide with BCG to inhibit bladder carcinoma.
abstract: There is growing interest in reducing Bacille Calmette-Guerin (BCG) side effects while keeping intact its therapeutic efficacy. In the present study, we evaluated the efficacy of Sclerotia of Polyporus umbellatus FRIES (Zhuling) and its main ingredient Polyporus Polysaccharide (PPS) to attenuate side effects of BCG therapy in vivo. The results show that bladder cancer development in model rats exhibited significantly reduced cancer invasiveness with Zhuling PPS combined with BCG. Flow cytometric (FCM) analysis showed expression of costimulatory molecules CD86, CD40, and TLR4/CD14 significantly increased with Zhuling PPS in combination with BCG. Similarly, immunohistochemical analysis revealed stronger CD86 and CD40 staining. Our findings show Zhuling PPS strongly reduced side effects and displayed synergistic effects during BCG instillation in rat bladder cancer models. The findings also suggest that the attenuation effect may result from direct activation of dendritic cell (DC) TLR4.
authors.affiliation: College of Chinese Medicine, Hebei University, Baoding 071002, China. Electronic address: xxzgw@126.com.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Zhang
authors.affiliation: College of Chinese Medicine, Hebei University, Baoding 071002, China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Qin
authors.affiliation: College of Chinese Medicine, Hebei University, Baoding 071002, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Han
authors.affiliation: The School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong Province, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Li
authors.affiliation: College of Chinese Medicine, Hebei University, Baoding 071002, China.
authors.forename: Hong-Gai
authors.initials: HG
authors.lastname: Yang
authors.affiliation: College of Chinese Medicine, Hebei University, Baoding 071002, China.
authors.forename: Pi-Hu
authors.initials: PH
authors.lastname: Nie
authors.affiliation: Guangdong Provincial Academy of Chinese Medical Sciences, Guangdong Provincial Traditional Chinese Medicine Hospital, Guangzhou 510006, Guangdong Province, China. Electronic address: zengxing-china@163.com.
authors.forename: Xing
authors.initials: X
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use* / Q000652:urine*; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D005260:Female; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D009163:Mycobacterium bovis / Q000502:physiology*; D055492:Polyporus / Q000737:chemistry*; D011134:Polysaccharides / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000628:therapy*
chemical_list: D000970:Antineoplastic Agents; D051940:B7-2 Antigen; D019013:CD40 Antigens; D018950:Lipopolysaccharide Receptors; D011134:Polysaccharides; D051197:Toll-Like Receptor 4
keywords: Bacille Calmette-Guerin; Bladder carcinoma; Polyporus polysaccharide; Sclerotia of Polyporus umbellatus FRIES