title: Poly(A)(+) mRNA-binding protein Tudor-SN regulates stress granules aggregation dynamics.
abstract: Stress granules (SGs) and processing bodies (PBs) comprise the main types of cytoplasmic RNA foci during stress. Our previous data indicate that knockdown of human Tudor staphylococcal nuclease (Tudor-SN) affects the aggregation of SGs. However, the precise molecular mechanism has not been determined fully. In the present study, we demonstrate that Tudor-SN binds and colocalizes with many core components of SGs, such as poly(A)(+) mRNA binding protein 1, T-cell internal antigen-1-related protein and poly(A)(+) mRNA, and SG/PB sharing proteins Argonaute 1/2, but not PB core proteins, such as decapping enzyme 1 a/b, confirming that Tudor-SN is an SG-specific protein. We also demonstrate that the Tudor-SN granule actively communicates with the nuclear and cytosolic pool under stress conditions. Tudor-SN can regulate the aggregation dynamics of poly(A)(+) mRNA-containing SGs and selectively stabilize the SG-associated mRNA during cellular stress.
authors.affiliation: Department of Biochemistry and Molecular Biology, Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, China; Laboratory of Molecular Immunology, Research Center of Basic Medical Science, Tianjin Medical University, China; Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin Medical University, China; Key Laboratory of Educational Ministry of China, Tianjin Medical University, China.
authors.forename: Xingjie
authors.initials: X
authors.lastname: Gao
authors.forename: Xue
authors.initials: X
authors.lastname: Fu
authors.forename: Juan
authors.initials: J
authors.lastname: Song
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoteng
authors.initials: X
authors.lastname: Cui
authors.forename: Chao
authors.initials: C
authors.lastname: Su
authors.forename: Lin
authors.initials: L
authors.lastname: Ge
authors.forename: Jie
authors.initials: J
authors.lastname: Shao
authors.forename: Lingbiao
authors.initials: L
authors.lastname: Xin
authors.forename: Juha
authors.initials: J
authors.lastname: Saarikettu
authors.forename: Mei
authors.initials: M
authors.lastname: Mei
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Minxin
authors.initials: M
authors.lastname: Wei
authors.forename: Olli
authors.initials: O
authors.lastname: Silvennoinen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Jinyan
authors.initials: J
authors.lastname: He
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
mesh_terms: D060565:Argonaute Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004265:DNA Helicases; D004720:Endonucleases; D004722:Endoribonucleases / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D011061:Poly A / Q000378:metabolism; D039122:Poly(A)-Binding Protein I / Q000378:metabolism; D000075223:Poly-ADP-Ribose Binding Proteins; D011132:Polyribosomes / Q000378:metabolism; D060066:Protein Interaction Maps; D020365:RNA Helicases; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D015534:Trans-Activators / Q000378:metabolism
chemical_list: C115848:AGO1 protein, human; D060565:Argonaute Proteins; D002352:Carrier Proteins; D039642:Eukaryotic Initiation Factors; C577903:IGFBP2 protein, human; D018971:Insulin-Like Growth Factor Binding Protein 2; D009687:Nuclear Proteins; D039122:Poly(A)-Binding Protein I; D000075223:Poly-ADP-Ribose Binding Proteins; D000067776:RNA Recognition Motif Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D011061:Poly A; D004720:Endonucleases; D004722:Endoribonucleases; C095221:SND1 protein, human; C466279:DCP1A protein, human; C467723:DCP2 protein, human; D004265:DNA Helicases; C116070:G3BP1 protein, human; D020365:RNA Helicases
keywords: PABP1; Tudor-SN; poly(A)+ mRNA; processing bodies; stress granules