title: A pliable electroporation patch (ep-Patch) for efficient delivery of nucleic acid molecules into animal tissues with irregular surface shapes.
abstract: Delivery of nucleic acids into animal tissues by electroporation is an appealing approach for various types of gene therapy, but efficiency of existing methodsis not satisfactory. Here we present the validation of novel electroporation patch (ep-Patch) for efficient delivery of DNA and siRNA into mouse tissues. Using micromachining technology, closely spaced gold electrodes were made on the pliable parylene substrate to form a patch-like electroporation metrics. It enabled large coverage of the target tissues and close surface contact between the tissues and electrodes, thus providing a uniform electric field to deliver nucleic acids into tissues, even beneath intact skin. Using this ep-Patch for efficiently delivery of both DNA and siRNA, non-invasive electroporation of healthy mouse muscle tissue was successfully achieved. Delivery of these nucleic acids was performed to intact tumors with satisfactory results. Silencing of tumor genes using the ep-Patch was also demonstrated on mice. This pliable electroporation patch method constitutes a novel way of in vivo delivery of siRNA and DNA to certain tissues or organs to circumvent the disadvantages of existing methodologies for in vivo delivery of nucleic acid molecules.
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Institute of Molecular Medicine, Peking University, Beijing 100871, PR China.
authors.forename: Yuanyu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Molecular Medicine, Peking University, Beijing 100871, PR China.
authors.forename: Deyao
authors.initials: D
authors.lastname: Zhao
authors.affiliation: National Center for Nanoscience and Technology, Beijing 100190, PR China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: National Key Laboratory of Science and Technology on Micro/Nano Fabrication, Institute of Microelectronics, Peking University, Beijing 100871, PR China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Molecular Medicine, Peking University, Beijing 100871, PR China.
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry; D045744:Cell Line, Tumor; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004566:Electrodes; D018274:Electroporation / Q000379:methods*; D017930:Genes, Reporter; D057809:HEK293 Cells; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000473:pathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*
chemical_list: D002232:Carbocyanines; D034741:RNA, Small Interfering; C085321:cyanine dye 5; D004247:DNA