title: MiR-540 as a novel adipogenic inhibitor impairs adipogenesis via suppression of PPARγ.
abstract: A better understanding of the molecular mechanisms in adipogenesis may provide new insights into adipose tissue-related diseases. Recently, microRNAs (miRNAs) have emerged as a class of epigenetic regulators of stem cell differentiation. In this study, we found that miR-540 was an essential negative regulator of adipogenic differentiation in adipose tissue-derived stromal cells (ADSCs). Lentivirus-mediated overexpression of miR-540 resulted in blockade of the expression of C/EBP-α and PPARγ, the two master transcription factors of adipogenesis, and deficient lipid accumulation, whereas inhibition of miR-540 promoted these processes. Target gene reporter assays showed that miR-540 directly targeted the 3'-untranslated region (3'UTR) of PPARγ, resulting in a decrease of PPARγ protein expression. Collectively, these data suggest that miR-540 represents a new adipogenic inhibitor by, at least in part, targeting PPARγ.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, P. R. China; Department of Oral and Maxillofacial Surgery, The Second Xiangya Hospital, Central South University, Changsha, 410011, P. R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Yuanwei
authors.initials: Y
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Lanfeng
authors.initials: L
authors.lastname: Ye
authors.forename: Junhui
authors.initials: J
authors.lastname: Cui
authors.forename: Quan
authors.initials: Q
authors.lastname: Sun
authors.forename: Kaide
authors.initials: K
authors.lastname: Li
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Wu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D047495:PPAR gamma
keywords: ADIPOGENIC DIFFERENTIATION; ADIPOSE TISSUE-DERIVED STROMAL CELLS; PPAR-γ; miR-540