title: Procoagulant phospholipid concentration in canine erythrocyte concentrates stored with or without prestorage leukoreduction.
abstract: OBJECTIVE
  To evaluate canine erythrocyte concentrates (ECs) for the presence of procoagulant phospholipid (PPL), determine whether PPL concentration changes during the course of storage of ECs, and ascertain whether prestorage leukoreduction (removal of leukocytes via gravity filtration) reduces the development of PPL.
  
  
  METHODS
  10 whole blood units (420 g each) collected from 10 random-source, clinically normal dogs (1 U/dog).
  
  
  METHODS
  The dogs were randomized to 1 of 2 groups. Of the 10 whole blood units collected, 5 were processed through a standard method, and 5 underwent leukoreduction. Whole blood units were processed to generate ECs, from which aliquots were aseptically collected from each unit weekly for 5 weeks. Supernatants from the concentrates were evaluated for procoagulant activity, which was converted to PPL concentration, by use of an automated assay and by measurement of real-time thrombin generation.
  
  
  RESULTS
  Supernatants from stored canine ECs contained procoagulant activity as measured by both assays. In general, the PPL concentration gradually increased during the storage period, but leukoreduction reduced the development of increased procoagulant activity over time.
  
  
  CONCLUSIONS
  The presence of PPL in canine ECs may be associated with procoagulant and proinflammatory effects in vivo, which could have adverse consequences for dogs treated with ECs.
authors.affiliation: Department of Biochemistry, School of Molecular and Cellular Biology, University of Illinois, Urbana, IL 61801.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Smith
authors.forename: Thandeka R
authors.initials: TR
authors.lastname: Ngwenyama
authors.forename: Mauria
authors.initials: M
authors.lastname: O'Brien
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Herring
authors.forename: Rafaella
authors.initials: R
authors.lastname: Corsi
authors.forename: Alyssa
authors.initials: A
authors.lastname: Galligan
authors.forename: Alison N
authors.initials: AN
authors.lastname: Beloshapka
authors.forename: Ping
authors.initials: P
authors.lastname: Deng
authors.forename: Kelly S
authors.initials: KS
authors.lastname: Swanson
authors.forename: Maureen
authors.initials: M
authors.lastname: McMichael
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D017707:Erythrocyte Transfusion / Q000662:veterinary*; D004912:Erythrocytes / Q000378:metabolism; D047589:Leukocyte Reduction Procedures / Q000662:veterinary; D010743:Phospholipids / Q000032:analysis
chemical_list: D010743:Phospholipids