title: Spectrally resolved confocal microscopy using lanthanide centred near-IR emission.
abstract: The narrow, near infrared (NIR) emission from lanthanide ions has attracted great interest, particularly with regard to developing tools for bioimaging, where the long lifetimes of lanthanide excited states can be exploited to address problems arising from autofluorescence and sample transparency. Despite the promise of lanthanide-based probes for near-IR imaging, few reports on their use are present in the literature. Here, we demonstrate that images can be recorded by monitoring NIR emission from lanthanide complexes using detectors, optical elements and a microscope that were primarily designed for the visible part of the spectrum.
authors.affiliation: Nano-Science Center & Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 København ø, Denmark. tom@chem.ku.dk TJS@chem.ku.dk.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Liao
authors.forename: Manuel
authors.initials: M
authors.lastname: Tropiano
authors.forename: Konstantins
authors.initials: K
authors.lastname: Mantulnikovs
authors.forename: Stephen
authors.initials: S
authors.lastname: Faulkner
authors.forename: Tom
authors.initials: T
authors.lastname: Vosch
authors.forename: Thomas Just
authors.initials: TJ
authors.lastname: Sørensen
mesh_terms: D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D018613:Microscopy, Confocal; D012822:Silicon Dioxide / Q000737:chemistry; D019265:Spectroscopy, Near-Infrared
chemical_list: D056831:Coordination Complexes; D028581:Lanthanoid Series Elements; D012822:Silicon Dioxide