title: Expression of papillary thyroid carcinoma-associated molecular markers and their significance in follicular epithelial dysplasia with papillary thyroid carcinoma-like nuclear alterations in Hashimoto's thyroiditis.
abstract: The aim of this study was to evaluate the expression of papillary thyroid carcinoma (PTC)-associated tumor markers in follicular epithelial dysplasia showing PTC-like nuclear alterations (FED) in Hashimoto's thyroiditis (HT) and to explore the relationship between HT and PTC. In this study, 43 PTC, 18 HT with FED and 16 peritumoral benign thyroid tissues were immunohistochemically analyzed for CK19, galectin-3, HBME-1, CD56, claudin-1 and NGAL expression. Our research revealed that in HT, the expression of CK19, galectin-3, HBME-1, claudin-1 and NGAL was focal and limited to FED, while CD56 was strongly positive in FED and most Hürthle cells. The stain intensity of CK19, claudin-1 and NGAL in FED decreased compared with PTC, but were significantly higher than that in peritumoral benign thyroid tissues (all P < 0.0125). For galectin-3, HBME-1 and CD56, no statistically significant difference was detected between HT and peritumoral benign thyroid tissues (all P > 0.05). In conclusion, In HT, FED might be a precancerous condition closely associated with PTC development as they have overlaps in cytological and immunomarker profiles, indicating that in patients with HT, under prolonged stimuli from chronic inflammation, part of follicular epithelia may show regeneration, hyperplasia, Hürthle cell metaplasia and dysplasia, eventually malignant transformation. Hence, long term follow-up and regular inspection would be necessary for Hashimoto's thyroiditis with FED.
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of ENT, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Lingzhi
authors.initials: L
authors.lastname: Qin
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei Province, China.
authors.forename: Naping
authors.initials: N
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000473:pathology; D062445:Claudin-1 / Q000378:metabolism; D005260:Female; D037502:Galectin 3 / Q000378:metabolism; D050031:Hashimoto Disease / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D053539:Keratin-19 / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013961:Thyroid Gland / Q000378:metabolism* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult; D000951:Antigens, Neoplasm
chemical_list: D014408:Biomarkers, Tumor; D062445:Claudin-1; D037502:Galectin 3; D053539:Keratin-19; C432845:HBME-1 antigen; D000951:Antigens, Neoplasm
keywords: CD56; CK19; HBME-1; Hashimoto’s thyroiditis; NGAL; Papillary thyroid carcinoma; claudin-1; galectin-3