title: Seroprevalence and seroconversion of dengue and implications for clinical diagnosis in amazonian children.
abstract: This study aimed to evaluate the prevalence of serum IgG dengue in children in an Amazonian population, to assess the seroconversion rate in 12 months, and to estimate how many seropositive children had a prior clinical diagnosis of dengue. We conducted a population-based study between 2010 and 2011, with children aged 6 months to 12 years that were living in the urban area of a small town in the Brazilian Amazon. The prevalence of IgG antibodies against dengue antigens was determined by indirect ELISA technique, and seronegative children were reexamined after 12 months to determine seroconversion rates. Results showed seroprevalence of IgG antibodies against dengue type of 2.9%, with no significant association between age, race, and sex. In seropositive children, only 8.4% had received a clinical diagnosis of dengue, and the ratio of clinically diagnosed cases and subclinical cases was 1 : 11. The seroconversion rate between 2010 and 2011 was 1.4% (CI 3.8% to 35.1%). The seroprevalence of dengue in this pediatric population was low, and the vast majority of cases were not clinically detected, suggesting a difficulty in making the clinical diagnosis in children and a high frequency of asymptomatic infections.
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Antonio Camargo
authors.initials: AC
authors.lastname: Martins
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Thasciany Moraes
authors.initials: TM
authors.lastname: Pereira
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Humberto
authors.initials: H
authors.lastname: Oliart-Guzmán
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Breno Matos
authors.initials: BM
authors.lastname: Delfino
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Saulo Augusto Silva
authors.initials: SA
authors.lastname: Mantovani
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Athos Muniz
authors.initials: AM
authors.lastname: Braña
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Fernando Luiz Cunha Castelo
authors.initials: FL
authors.lastname: Branco
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: José Alcântara
authors.initials: JA
authors.lastname: Filgueira Júnior
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Santos
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Alanderson Alves
authors.initials: AA
authors.lastname: Ramalho
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Andréia Silva
authors.initials: AS
authors.lastname: Guimarães
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Thiago Santos
authors.initials: TS
authors.lastname: de Araújo
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Cristieli Sérgio de Menezes
authors.initials: CS
authors.lastname: Oliveira
authors.affiliation: Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.
authors.forename: Benedito Antônio Lopes
authors.initials: BA
authors.lastname: da Fonseca
authors.affiliation: Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Campus Universitário, BR 364, Bairro Distrito Industrial, 69.919-900 Rio Branco, AC, Brazil.
authors.forename: Mônica
authors.initials: M
authors.lastname: da Silva-Nunes