title: microRNA profiling in three main stages during porcine spermatogenesis.
abstract: BACKGROUND
  Spermatogenesis is an intricate biological event wherein an undifferentiated spermatogonium develops into mature sperms. MicroRNAs are a type of single strand small non-coding RNA molecule and are implicated in the regulation of many crucial pathways during cell proliferation, apoptosis, and differentiation.
  
  
  METHODS
  Here, we present a comprehensive comparison of miRNA expression profiling in three main stages during porcine spermatogenesis using high-throughput sequencing.
  
  
  RESULTS
  We built three small RNA libraries for the testis, the epididymis and the ejaculated sperm from a Landrace boar, and in total obtained 3821 precursor hairpins encoding for 4761 mature miRNAs, of which 23 are miRNA*. Notably, 940 precursor miRNAs produced both the 5'- and 3'- strands as sister pairs, indicating the distinctive expression patterns of germ cell miRNAs. Additionally, 418 out of 710 co-expressed miRNAs were identified as being differentially expressed between libraries (P < 0.001). Apart from the sexual specific X chromosome, many miRNAs were found to be located on chromosome 12, which may play potential roles in spermatogenesis according to the result of synteny analysis with human and mouse. The Gene Ontology and KEGG pathway analysis revealed that the target genes of co-expressed miRNAs were highly involved in the cell cycle process, metal ion binding, modification of plasma membrane, and the p53 signal pathway.
authors.affiliation: Department of Animal Science, Southwest University, Rongchang, Chongqing, China, luozgg@163.com.
authors.forename: Zonggang
authors.initials: Z
authors.lastname: Luo
authors.forename: Yingkai
authors.initials: Y
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Michael
authors.initials: M
authors.lastname: Ellis
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Li
authors.forename: Jinyong
authors.initials: J
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Penghui
authors.initials: P
authors.lastname: Fu
authors.forename: Ketian
authors.initials: K
authors.lastname: Wang
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D006801:Humans; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D013091:Spermatogenesis / Q000235:genetics*; D013552:Swine; D013737:Testis / Q000254:growth & development*
chemical_list: D035683:MicroRNAs