title: Laboratory and clinical features of abnormal macroenzymes found in human sera.
abstract: We report the analysis of unusual macroenzymes, performed in our laboratory, and review the relevant literature. In particular, we focused on macro AST, macroamylase, macro LD and macro CK. Macroenzymes are seen in healthy subjects, but can also be related to disease; thus, accurate detection is useful in day-to-day clinical practice. The macroenzyme is thought to be a specific antigen-antibody complex from the following findings: (1) the complex could be dissociated under acidic pH levels; (2) binding specificity of immunoglobulin in the complex was observed; (3) the binding site of immunoglobulin in the complex was Fab portion; and (4) the maternal IgG involved with macroenzyme was transferred to her children. This article is part of a Special Issue entitled: Medical Proteomics.
authors.affiliation: Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita-12 Nishi-5, Kita-ku, Sapporo 0600812, Japan. Electronic address: moriyama@hs.hokudai.ac.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Moriyama
authors.affiliation: Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, 1110 Shimokatou, Chuo 4093898, Japan; Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo, 1020083 Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Tamura
authors.affiliation: Clinical Laboratory and Blood Center, Hokkaido University Hospital, Kita-14 Nishi-5, Kita-ku, Sapporo 0600814, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Clinical Laboratory and Blood Center, Asahikawa Medical College Hospital, 2-1-1-1 Midorigaoka, Asahikawa 0788510, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Otsuka
authors.affiliation: First Department of Medicine, Hokkaido University School of Medicine, Kita-14 Nishi-5, Kita-ku, Sapporo 0600814, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Shigemura
authors.affiliation: Medical Laboratory Science, Faculty of Health Sciences, Hokkaido University, Kita-12 Nishi-5, Kita-ku, Sapporo 0600812, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Honma
mesh_terms: D000681:Amylases / Q000097:blood*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood*; D001219:Aspartate Aminotransferases / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007140:Immunoglobulin Fab Fragments / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*
chemical_list: D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000681:Amylases
keywords: Macro aspartate aminotransferase (AST); Macro creatine kinase (CK); Macro lactate dehydrogenase (LD); Macroamylase; Macroenzyme