title: The "kidney-liver" multiorgan ex vivo perfused model improves the circuit's biochemical milieu during perfusion compared to the "liver-kidney" counterpart.
abstract: The multiorgan ex vivo perfused liver-kidney model allows studying the hepatic pathophysiology and purifying waste products. We tested if the addition of the kidney first followed by the liver (KL circuit) produces better results compared to the classic liver-first approach (LK). Intact livers and kidneys were obtained post mortem from ten female domestic white pigs, five experiments were conducted with the KL circuit and five with the LK. Bile, urine production, arterial blood gases, glucose, renal and liver tests were collected hourly during the perfusions. The KL circuit had values more close to physiological ranges, more stable over time and showed less variability compared to the LK circuit for urine production, glucose, PH, anion gap, lactate, urea, sodium, potassium and Alanine Transaminase (ANOVA test for repeated measures p < 0.05). The KL circuit produced a more physiological and reliable biochemical milieu.
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, Leicester General Hospital, University of Leicester, Gwendolen Road, Leicester, LE5 4PW, UK, wenchung813@btinternet.com.
authors.forename: Wen Yuan
authors.initials: WY
authors.lastname: Chung
authors.forename: Gianpiero
authors.initials: G
authors.lastname: Gravante
authors.forename: Amar
authors.initials: A
authors.lastname: Eltweri
authors.forename: Roberto
authors.initials: R
authors.lastname: Sorge
authors.forename: Seok Ling
authors.initials: SL
authors.lastname: Ong
authors.forename: Cristina
authors.initials: C
authors.lastname: Pollard
authors.forename: Mathew
authors.initials: M
authors.lastname: Metcalfe
authors.forename: Ashley
authors.initials: A
authors.lastname: Dennison
mesh_terms: D000818:Animals; D005260:Female; D005947:Glucose / Q000378:metabolism; D006464:Hemoperfusion / Q000379:methods*; D007668:Kidney / Q000502:physiology*; D007677:Kidney Function Tests; D008099:Liver / Q000502:physiology*; D008111:Liver Function Tests; D023421:Models, Animal; D013552:Swine
chemical_list: D005947:Glucose