title: Overexpression of the Circadian Clock Gene Rev-erbα Affects Murine Bone Mesenchymal Stem Cell Proliferation and Osteogenesis.
abstract: Bone mesenchymal stem cell (BMSC) age-related changes include decreased osteogenesis and increased adipogenesis. Rev-erbα and the Wnt/β-catenin signaling pathway were known to play important roles in BMSC aging. In this study, we have aimed to elucidate whether Rev-erbα and Wnt/β-catenin signaling interact during BMSC proliferation and osteogenesis. Our results showed that Rev-erbα expression gradually dropped during BMSC osteogenesis, and overexpression of Rev-erbα in BMSCs inhibited cell proliferation and osteogenesis. The inhibition of cell proliferation induced by Rev-erbα overexpression was partially reversed when Wnt/β-catenin signaling was activated. These results suggested that Rev-erbα could promote BMSC aging and may be the negative regulator during the late stage of osteogenesis. The clock gene Rev-erbα and Wnt/β-catenin signaling interact in the regulation of cell proliferation.
authors.affiliation: 1 Orthodontic Centre, West China College of Stomatology, Sichuan University , Chengdu, China .
authors.forename: Yao
authors.initials: Y
authors.lastname: He
authors.forename: Fuwei
authors.initials: F
authors.lastname: Lin
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tan
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D056926:CLOCK Proteins / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D057906:Circadian Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008810:Mice, Inbred C57BL; D010012:Osteogenesis / Q000502:physiology*; D015398:Signal Transduction; D051176:beta Catenin / Q000235:genetics
chemical_list: D051176:beta Catenin; D056926:CLOCK Proteins; C538944:Clock protein, mouse