title: Increased frequencies of nuocytes in peripheral blood from patients with Graves' hyperthyroidism.
abstract: Newly identified nuocytes play an important role in Th2 cell mediated immunity such as protective immune responses to helminth parasites, allergic asthma and chronic rhinosinusitis. However, the contributions of nuocytes in the occurrence and development of Graves' hyperthyroidism remains unknown. Previous studies found that there was a predominant Th2 phenotype in patients with Graves' hyperthyroidism, it might relate to polarization of nuocytes. Nuocytes were defined by transcription factor RORα, various cell surface markers (T1/ST2, IL-17RB, ICOS, CD45) and associated cytokines. In this study, these cells related genes or molecules in PBMC from patients with Graves' hyperthyroidism were measured, and the potential correlation between them was analyzed. The expression levels of T1/ST2, IL-17RB, ICOS, IL-5 and IL-13, which represented nuocytes associated molecules were significantly increased in patients, meanwhile, the RORα mRNA also had a tendency to increase. In addition, IFN-γ and T-bet (Th1 related cytokine and transcription factor) were obviously decreased, and there was a positive correlation between IL-17RB and IL-13. These results suggested that there were polarized nuocytes in Graves' hyperthyroidism patients, and which closely related to the down-regulation of Th1 cells or relatively advantage of Th2 differentiation.
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Bie
authors.affiliation: Department of Endocrinology, The Affiliated People's Hospital of Jiangsu University Zhenjiang 212001, PR China.
authors.forename: Yingzhao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, The Affiliated People's Hospital of Jiangsu University Zhenjiang 212001, PR China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Ying
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Huijian
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University Zhenjiang 212013, PR China.
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006111:Graves Disease / Q000097:blood* / Q000276:immunology; D006801:Humans; D018793:Interleukin-13 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D055815:Young Adult
chemical_list: D018793:Interleukin-13; D015848:Interleukin-5
keywords: Graves’ hyperthyroidism; Nuocytes; cytokines; transcription factors