title: Single nucleotide polymorphisms in VEGF gene are associated with an increased risk of osteosarcoma.
abstract: We aimed to assess whether the five common SNPs can affect the risk of osteosarcoma, and its association with demographic characteristics of osteosarcoma. 165 osteosarcoma patients and 330 cancer-free controls were enrolled into our study. Five common SNPs in VEGF gene, -2578C/A (rs699947), -1156G/A (rs1570360), +1612G/A (rs10434), +936C/T (rs3025039) and -634G/C (rs2010963), were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Conditional logistic regression analyses found that individuals with AA genotype and A allele of rs699947 were associated with an increased risk of osteosarcoma. Individuals with GG genotype and G allele of rs2010963 were associated with an increased risk of osteosarcoma. By stratified analysis, AA genotype of rs699947 was associated with an increased risk of osteosarcoma in those with shorter age, males and a family history of cancer, and GG genotype of rs2010963 was correlated with an increased risk of osteosarcoma in those with shorter age, females and a family history of cancer. Our study suggests that rs699947 and rs2010963 polymorphisms may play a role in the pathogenesis of osteosarcoma.
authors.affiliation: Department of Human Anatomy Histology and Embryology, School of Basic Medicine, Capital Medical University Beijing, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Tie
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Zhongwen
authors.initials: Z
authors.lastname: Zhai
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Zhenqun
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Human Anatomy Histology and Embryology, School of Basic Medicine, Capital Medical University Beijing, China.
authors.forename: Deshan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Pediatric Orthopedics, The Second Affiliated Hospital of Inner Mongolia Medical University Hohhot, China.
authors.forename: Wanlin
authors.initials: W
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000483:Alleles; D001859:Bone Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012737:Sex Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*; D055815:Young Adult
chemical_list: D042461:Vascular Endothelial Growth Factor A
keywords: Single nucleotide polymorphism; osteosarcoma; vascular endothelial growth factor