title: [The effects of ADAM28 AS-ODN on biological function of human gingival fibroblasts].
abstract: OBJECTIVE
  To study the effects of a disintegrin and metalloproteinase 28 (ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, differentiation and apoptosis of human gingival fibroblasts (HGFs), and analyze the possible mechanism.
  
  
  METHODS
  Cell culture, gene transfection, MTT chromatometry, enzyme dynamics, and flow cytometry (FCM) techniques were used to detect the effects of ADAM28 AS-ODN on biological characteristics of HGFs after transfected into HGFs. The statistical differences were evaluated by SNK test with SPSS 16.0 software package.
  
  
  RESULTS
  In ADAM28 AS-ODN group, the proliferation activity of HGFs decreased significantly. Cell percentage in S phase in AS-ODN group was notably lower than that of S-ODN and untransfected groups, and cell percentage in G2+M phase was remarkably lower than that of untransfected group. Cell proliferation index (PI=S+G2M) in AS-ODN group was significantly lower than that of the other two groups. There was a significant difference between the groups. In AS-ODN group, alkaline phosphatase (ALP) secretion activity and percentage of apoptotic cell notably increased.
  
  
  CONCLUSIONS
  ADAM28 AS-ODN could inhibit HGFs proliferation significantly and influence the changes of cell cycle, promote HGFs differentiation and induce HGFs apoptosis remarkably.
authors.affiliation: Department of Stomatology, the 401 Hospital of Chinese PLA. Qingdao 266071, Shandong Province, China.E-mail:zhaozheng170@163.com.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.forename: Lu-Jia
authors.initials: LJ
authors.lastname: Li
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Zheng-nan
authors.initials: ZN
authors.lastname: Huang
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Yang
mesh_terms: D051722:ADAM Proteins / Q000502:physiology*; D017209:Apoptosis; D002453:Cell Cycle; D002454:Cell Differentiation; D049109:Cell Proliferation; D005347:Fibroblasts; D006801:Humans; D014162:Transfection
chemical_list: D051722:ADAM Proteins; C410224:ADAM28 protein, human