title: Acute simulated soccer-specific training increases PGC-1α mRNA expression in human skeletal muscle.
abstract: The aim of the current study was to quantify oxygen uptake, heart rate and molecular responses of human skeletal muscle associated with mitochondrial biogenesis following an acute bout of simulated soccer training. Muscle biopsies (vastus lateralis) were obtained from nine active men immediately pre-completion, post-completion and 3 h post-completion of a laboratory-based soccer-specific training simulation on a motorised treadmill. The soccer-specific simulation was a similar intensity (55 ± 6% [Formula: see text]) and duration (60 min) as that observed in professional soccer training (e.g. standing 41%, walking 37%, jogging 11%, high-speed running 9% and sprinting 2%). Post-exercise, muscle glycogen decreased (Pre; 397 ± 86 mmol∙kg(-1) dw, Post; 344 ± 64 mmol∙kg(-1) dw; P = 0.03), plasma lactate increased (P < 0.001) up to ~4-5 mmol∙L(-1), non-esterified fatty acids and glycerol increased (P < 0.001) to values of 0.6 ± 0.2 mmol∙L(-1) and 145 ± 54 μmol∙L(-1), respectively. PGC-1α mRNA increased (P = 0.009) fivefold 3 h post-exercise. We provide novel data by demonstrating that soccer-specific training is associated with increases in PGC-1α mRNA. These data may have implications for practitioners in better understanding the metabolic and muscle responses to soccer-specific training protocols in the field.
authors.affiliation: a Research Institute for Sport and Exercise Sciences , Liverpool John Moores University , Liverpool , UK.
authors.forename: Tae-Seok
authors.initials: TS
authors.lastname: Jeong
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Bartlett
authors.forename: Chang-Hwa
authors.initials: CH
authors.lastname: Joo
authors.forename: Jari
authors.initials: J
authors.lastname: Louhelainen
authors.forename: Graeme L
authors.initials: GL
authors.lastname: Close
authors.forename: James P
authors.initials: JP
authors.lastname: Morton
authors.forename: Barry
authors.initials: B
authors.lastname: Drust
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000222:Adaptation, Physiological; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D005227:Fatty Acids / Q000097:blood; D005990:Glycerol / Q000097:blood; D006003:Glycogen / Q000378:metabolism; D006339:Heart Rate; D006801:Humans; D019344:Lactic Acid / Q000097:blood; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D010101:Oxygen Consumption; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010766:Phosphorylation; D010806:Physical Education and Training / Q000379:methods*; D012333:RNA, Messenger / Q000378:metabolism*; D012916:Soccer / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*; D055815:Young Adult; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D005227:Fatty Acids; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D012333:RNA, Messenger; D014157:Transcription Factors; D019344:Lactic Acid; D006003:Glycogen; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases; D005990:Glycerol
keywords: cell signalling; intermittent exercise; vastus lateralis