title: F-ATPase of Drosophila melanogaster forms 53-picosiemen (53-pS) channels responsible for mitochondrial Ca2+-induced Ca2+ release.
abstract: Mitochondria of Drosophila melanogaster undergo Ca(2+)-induced Ca(2+) release through a putative channel (mCrC) that has several regulatory features of the permeability transition pore (PTP). The PTP is an inner membrane channel that forms from F-ATPase, possessing a conductance of 500 picosiemens (pS) in mammals and of 300 pS in yeast. In contrast to the PTP, the mCrC of Drosophila is not permeable to sucrose and appears to be selective for Ca(2+) and H(+). We show (i) that like the PTP, the mCrC is affected by the sense of rotation of F-ATPase, by Bz-423, and by Mg(2+)/ADP; (ii) that expression of human cyclophilin D in mitochondria of Drosophila S2R(+) cells sensitizes the mCrC to Ca(2+) but does not increase its apparent size; and (iii) that purified dimers of D. melanogaster F-ATPase reconstituted into lipid bilayers form 53-pS channels activated by Ca(2+) and thiol oxidants and inhibited by Mg(2+)/γ-imino ATP. These findings indicate that the mCrC is the PTP of D. melanogaster and that the signature conductance of F-ATPase channels depends on unique structural features that may underscore specific roles in different species.
authors.affiliation: From the Departments of Biomedical Sciences and.
authors.forename: Sophia
authors.initials: S
authors.lastname: von Stockum
authors.affiliation: From the Departments of Biomedical Sciences and.
authors.forename: Valentina
authors.initials: V
authors.lastname: Giorgio
authors.affiliation: From the Departments of Biomedical Sciences and.
authors.forename: Elena
authors.initials: E
authors.lastname: Trevisan
authors.affiliation: the Department of Food Science, University of Udine, I-33100 Udine, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lippe
authors.affiliation: the Department of Chemistry, Graduate Program in Immunology, University of Michigan, Ann Arbor, Michigan 48109, and.
authors.forename: Gary D
authors.initials: GD
authors.lastname: Glick
authors.affiliation: the Vollum Institute, Oregon Health and Sciences University, Portland, Oregon 97239.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Forte
authors.affiliation: Biology, University of Padova and Consiglio Nazionale delle Ricerche Neuroscience Institute, I-35131 Padova, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Da-Rè
authors.affiliation: Biology, University of Padova and Consiglio Nazionale delle Ricerche Neuroscience Institute, I-35131 Padova, Italy.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Checchetto
authors.affiliation: Biology, University of Padova and Consiglio Nazionale delle Ricerche Neuroscience Institute, I-35131 Padova, Italy.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Mazzotta
authors.affiliation: Biology, University of Padova and Consiglio Nazionale delle Ricerche Neuroscience Institute, I-35131 Padova, Italy.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Costa
authors.affiliation: Biology, University of Padova and Consiglio Nazionale delle Ricerche Neuroscience Institute, I-35131 Padova, Italy.
authors.forename: Ildikò
authors.initials: I
authors.lastname: Szabò
authors.affiliation: From the Departments of Biomedical Sciences and. Electronic address: bernardi@bio.unipd.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Bernardi
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D020013:Calcium Signaling / Q000502:physiology*; D002460:Cell Line; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D006801:Humans; D033681:Mitochondrial Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore
chemical_list: D015220:Calcium Channels; D029721:Drosophila Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D000251:Adenosine Triphosphatases; D002118:Calcium
keywords: Calcium Transport; Drosophila; Mitochondria; Permeability; calcium