title: Different angiogenic abilities of self-setting calcium phosphate cement scaffolds consisting of different proportions of fibrin glue.
abstract: To investigate the different angiogenic abilities of the self-setting calcium phosphate cement (CPC) consisting of different proportions of fibrin glue (FG), the CPC powder and the FG solution were mixed at the powder/liquid (P/L) ratios of 1 : 0.5, 1 : 1, and 1 : 2 (g/mL), respectively, and pure CPC was used as a control. After being implanted into the lumbar dorsal fascia of the rabbit, the angiogenic process was evaluated by histological examination and CD31 immunohistochemistry to detect the new blood vessels. The result of the new blood vessel showed that the P/L ratio of 1 : 1 group indicated the largest quantity of new blood vessel at 4 weeks, 8 weeks, and 12 weeks after implantation, respectively. The histological evaluation also showed the best vascular morphology in the 1 : 1 group at 4 weeks, 8 weeks, and 12 weeks after the operation, respectively. Our study indicated that the CPC-FG composite scaffold at the P/L ratio of 1 : 1  (g/mL) stimulated angiopoiesis better than any other P/L ratios and has significant potential as the bioactive material for the treatment of bone defects.
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Street, Xi'an 710032, China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Xiu
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Street, Xi'an 710032, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Fan
authors.affiliation: Institute of Gynaecology and Obstetrics, General Hospital of PLA, Beijing 100853, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Orthopaedics, General Hospital of PLA, Beijing 100853, China.
authors.forename: Geng
authors.initials: G
authors.lastname: Cui
authors.affiliation: Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, No. 127 Changle West Street, Xi'an 710032, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D001843:Bone Cements / Q000494:pharmacology; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D003738:Dental Cements / Q000737:chemistry / Q000494:pharmacology; D005269:Femur / Q000187:drug effects / Q000254:growth & development*; D015718:Fibrin Tissue Adhesive / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008422:Materials Testing; D011817:Rabbits
chemical_list: D001843:Bone Cements; D002130:Calcium Phosphates; D003738:Dental Cements; D015718:Fibrin Tissue Adhesive; C020243:calcium phosphate