title: Expression of α1-acid glycoprotein and lipopolysaccharide binding protein in visceral and subcutaneous adipose tissue of dairy cattle.
abstract: Adipose tissue is an endocrine compartment that plays an important role in immune defence by producing and releasing a wide range of proteins, including acute phase proteins (APPs). The liver is the main organ of APP synthesis, although extrahepatic production has also been reported. In the present study, expression of two APPs in dairy cattle, lipopolysaccharide binding protein (LBP) and α1-acid glycoprotein (AGP), was determined in four visceral (pericardial, mesenteric, omental and retroperitoneal) and three subcutaneous (withers, tail head and sternum) adipose tissue depots. mRNA expression was evaluated using qualitative and quantitative PCR, protein profiles were assessed by Western blot analysis and cellular localisation was determined by immunohistochemistry. The presence of LBP and AGP was demonstrated at mRNA and protein levels in all seven adipose tissue depots. Expression of AGP and LBP suggests that they may have roles as local and systemic inflammatory adipokines.
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany.
authors.forename: Mizanur M
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Department of Veterinary Science and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lecchi
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Sauerwein
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Mielenz
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Häußler
authors.affiliation: Department of Veterinary Science and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Restelli
authors.affiliation: Department of Veterinary Science and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Giudice
authors.affiliation: Department of Veterinary Science and Public Health, Università di Milano, Via Celoria 10, 20133 Milano, Italy. Electronic address: fabrizio.ceciliani@unimi.it.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Ceciliani
mesh_terms: D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism; D009961:Orosomucoid / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D050151:Subcutaneous Fat / Q000378:metabolism*; D000099231:Lipopolysaccharide-Binding Protein
chemical_list: D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D009961:Orosomucoid; D012333:RNA, Messenger; D000099231:Lipopolysaccharide-Binding Protein
keywords: Acute phase proteins; Adipose tissue; Bovine; Lipopolysaccharide binding proteins; α1-Acid glycoprotein