title: Octylphenol induces changes in glycosylation pattern, calcium level and ultrastructure of bank vole spermatozoa in vitro.
abstract: Our previous studies revealed that in bank vole testicular cells octylphenol (OP) action is related either to its binding to estrogen receptors or increasing cAMP level that modulates spermatozoa motility and acrosome reaction (AR), (Kotula-Balak et al., 2011, 2014). To better understand the mechanisms underlying these changes, in the present study we aimed at evaluating the glycosylation pattern, calcium (Ca(2+)) level and ultrastructure of OP-treated vole spermatozoa. Glycans were recognized by lectins and localized for the first time on the surface of acrosomal cap and tail of vole spermatozoa. Expression and localization of glycans were determined histochemically and analyzed quantitatively. Following OP the expression of glycans markedly changed with concomitant increase of intracellular Ca(2+) concentration. Altered Ca(2+) signaling pathway and ultrastructural changes in sperm acrosome region were revealed by immunoenzymatic assay and electron microscope analysis together with hypo-osmotic swelling test, respectively. In detail, AR advancement reflected by the presence of small vesicles in the close vicinity to the sperm head was observed, while tail membrane integrity remained intact. Our study provides a new insight on OP direct effects on precocious AR of vole spermatozoa through modulation of the glycan expression and its distribution concomitantly with changes in Ca(2+) signaling pathway and acrosome ultrastructure in vitro.
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland. Electronic address: malgorzata.kotula-balak@uj.edu.pl.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kotula-Balak
authors.affiliation: Department of Developmental Biology and Morphology of Invertebrates, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors.forename: Waclaw
authors.initials: W
authors.lastname: Tworzydlo
authors.affiliation: Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Pochec
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Zarzycka
authors.affiliation: Department of Endocrinology, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Bilinska
mesh_terms: D000818:Animals; D003411:Arvicolinae / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane; D006031:Glycosylation / Q000187:drug effects*; D066298:In Vitro Techniques; D037102:Lectins / Q000378:metabolism; D008297:Male; D009997:Osmotic Pressure; D010636:Phenols / Q000494:pharmacology*; D013082:Sperm Tail / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D037102:Lectins; D010636:Phenols; C474055:octylphenol; D002118:Calcium
keywords: Bank vole; Glycosylation pattern; Intracellular Ca(2+) concentration; Octylphenol; Spermatozoa; Ultrastructure