title: Isolation, characterization, and in rats plasma pharmacokinetic study of a new triterpenoid saponin from Dianthus superbus.
abstract: One new oleanolic acid triterpenoid saponin, 3-O-β-D-glucopyranosyl olean-11, 13(18)-diene-23,28-dioic acid, (hereafter referred to as DS-1) was isolated from the traditional Chinese medicinal plant Dianthus superbus (D. superbus). DS-1 plays an important role in the bioactivity of D. superbus. Thus, a sensitive, reliable and accurate reversed-phased liquid chromatography with tandem mass spectrometry (LC-MS/MS) in negative ion mode was developed and validated for the quantification and pharmacokinetic study of DS-1 in rats plasma. The pharmacokinetic profile showed that DS-1 was rapidly absorbed and eliminated in plasma, indicating that significant accumulation of the compound in biological specimen is unlikely. In addition, poor absorption into systemic circulation was observed after oral administration of DS-1, resulting in low absolute bioavailability (0.92 %).
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yina
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xiaobao
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Qianlan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Yongzhuang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Changshu Qiushi Technology Co. Ltd., Changshu, Jiangsu, China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang, China. tjk@zju.edu.cn.|Zhejiang University, Room 106, Zhou Yiqing Building, Zheda Road 38, Xihu district, Hangzhou, 310027, Zhejiang, China. tjk@zju.edu.cn.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid; D029749:Dianthus / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry
chemical_list: C000616652:3-O-glucopyranosylolean-11,13(18)-diene-23,28-dioic acid; D004365:Drugs, Chinese Herbal; D012503:Saponins
keywords: Dianthus superbus; LC–MS/MS; Pharmacokinetic; Triterpenoid saponin