title: Nucleoredoxin promotes adipogenic differentiation through regulation of Wnt/β-catenin signaling.
abstract: Nucleoredoxin (NRX) is a member of the thioredoxin family of proteins that controls redox homeostasis in cell. Redox homeostasis is a well-known regulator of cell differentiation into various tissue types. We found that NRX expression levels were higher in white adipose tissue of obese ob/ob mice and increased in the early adipogenic stage of 3T3-L1 preadipocyte differentiation. Knockdown of NRX decreased differentiation of 3T3-L1 cells, whereas overexpression increased differentiation. Adipose tissue-specific NRX transgenic mice showed increases in adipocyte size as well as number compared with WT mice. We further confirmed that the Wingless/int-1 class (Wnt)/β-catenin pathway was also involved in NRX-promoted adipogenesis, consistent with a previous report showing NRX regulation of this pathway. Genes involved in lipid metabolism were downregulated, whereas inflammatory genes, including those encoding macrophage markers, were significantly upregulated, likely contributing to the obesity in Adipo-NRX mice. Our results therefore suggest that NRX acts as a novel proadipogenic factor and controls obesity in vivo.
authors.affiliation: Department of Biological Science, Korea Advanced Institute Science and Technology (KAIST), Daejeon 305-701, Republic of Korea Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Young Jae
authors.initials: YJ
authors.lastname: Bahn
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Kwang-Pyo
authors.initials: KP
authors.lastname: Lee
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Jeong Yi
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Department of Biological Science, Korea Advanced Institute Science and Technology (KAIST), Daejeon 305-701, Republic of Korea.
authors.forename: Yeon-Soo
authors.initials: YS
authors.lastname: Seo
authors.affiliation: Aging Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Republic of Korea.
authors.forename: Ki-Sun
authors.initials: KS
authors.lastname: Kwon
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D060449:Wnt Signaling Pathway / Q000235:genetics / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; D051176:beta Catenin; D010088:Oxidoreductases; C105231:nucleoredoxin
keywords: Wingless/int-1 class (Wnt) signaling; adipogenesis; inflammation; obesity