title: Identification of the tropomyosin (HL-Tm) in Haemaphysalis longicornis.
abstract: Haemaphysalis longicornis tropomyosin (HL-Tm) was amplified by RT-PCR. The cDNA contained a 825 bp open reading frame coding for 274 amino acids with a predicted theoretical isoelectric point (pI) of 4.55 and molecular weight of 31.7 kDa. Real-time RT-PCR analysis showed that the expression levels of the HL-Tm in the unfed-females were significantly higher than in other tested developmental stages (eggs, unfed-larvae and unfed-nymphs). Western blot analysis showed that rabbit anti-serum against H. longicornis unfed-adult ticks recognized the recombinant HL-Tm protein (rHL-Tm). Immunization of rabbits with the rHL-Tm resulted in a statistically significant reduction of female engorgement and oviposition. Silencing of HL-Tm by RNAi showed a decrease in tick engorgement and oviposition, which is consistent with the effect of recombinant protein vaccine on the adults. These results showed that tick HL-Tm might be involved in the regulation of ticks blood-feeding, growth and oviposition.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Meiyuan
authors.initials: M
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Zhancheng
authors.initials: Z
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Junren
authors.initials: J
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yin
authors.affiliation: Wuwei Bureau of Animal Husbandry and Veterinary, Wuwei, Gansu Province 733000, People's Republic of China.
authors.forename: Qiaoying
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Chai
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping, Yanchangbu, Lanzhou, Gansu Province 730046, People's Republic of China. Electronic address: liuguangyuan2002@sina.com.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007114:Immunization; D026863:Ixodidae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D010058:Oviposition / Q000235:genetics; D034622:RNA Interference; D011817:Rabbits; D014335:Tropomyosin / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*
chemical_list: D014335:Tropomyosin
keywords: Haemaphysalis longicornis; Tropomyosin; Vaccination