title: A histochemical comparison of methyl green-pyronin, and hematoxylin and eosin for detecting apoptotic cells in oral squamous cell carcinoma, oral leukoplakia, oral submucous fibrosis and normal oral mucosa.
abstract: Analysis of apoptotic cells in oral pathological states could be useful for determining the rates of tissue turnover, which would help determine prognosis. The use of histochemical stains such as hematoxylin and eosin (H & E) and methyl green-pyronin (MGP) can provide a simple and cost-effective method for detecting apoptotic cells. We compared the efficacy of MGP and H & E for detecting apoptotic cells in oral squamous cell carcinoma (OSCC), oral leukoplakia (OL), oral submucous fibrosis (OSMF) and normal oral mucosa (NOM). Ten cases each of OSCC, OSMF, OL and NOM were retrieved from the archives and two serial sections were stained, one with H & E and the other with MGP. Apoptotic cells were identified at 100 x magnification and the apoptotic index was calculated. Apoptotic cells were distinguished more readily in MGP stained sections than in those stained with H & E. Also, the apoptotic cell count was greater in OSCC compared to OL, OSMF and NOM. We concluded that MGP staining can be used as a routine, cost-effective method for detecting apoptotic cells.
authors.affiliation: Department of Oral Pathology and Microbiology, Maratha Mandal NG Halgekar Institute of Dental Sciences and Research Centre , Belgaum 590010 Karnataka , India.
authors.forename: S
authors.initials: S
authors.lastname: Sumedha
authors.forename: V S
authors.initials: VS
authors.lastname: Kotrashetti
authors.forename: P
authors.initials: P
authors.lastname: Somannavar
authors.forename: R
authors.initials: R
authors.lastname: Nayak
authors.forename: D
authors.initials: D
authors.lastname: Babji
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000473:pathology; D004801:Eosine Yellowish-(YS) / Q000737:chemistry*; D006416:Hematoxylin / Q000737:chemistry*; D006801:Humans; D007972:Leukoplakia, Oral / Q000175:diagnosis / Q000473:pathology; D008739:Methyl Green / Q000737:chemistry*; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000473:pathology*; D009914:Oral Submucous Fibrosis / Q000175:diagnosis / Q000473:pathology
chemical_list: D008739:Methyl Green; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin
keywords: apoptosis; eosin; hematoxylin; light microscopy; methyl green; oral leukoplakia; oral squamous cell carcinoma; oral submucous fibrosis; pyronin