title: [Pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease by a GeXP™-based multiplex reverse transcription-PCR assay in Jinan, China, 2009-2012].
abstract: We investigated the pathogenic spectrum of enteroviruses associated with hand, foot and mouth disease (HFMD) in Jinan, China. A total of 274 specimens with a clinical diagnosis of HFMD in Jinan from 2009 to June 2012 were used. A GenomeLab™ (GeXP)-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay was employed to simultaneously detect 15 serotypes of human enteroviruses: human enterovirus (EV)71; coxsackievirus A (CVA)16, 4, 5, 6, 9, and 10; CVB1, 3 and 5; echovirus (Echo) 6, 7, 11, 13 and 19. Results showed that all samples were enterovirus-positive, with the most common serotypes being EV71 (25.18%) and CVA16 (16.06%), followed by CVA10 (14.23%), CVA6 (7.30%), CVB1 (1.09%), Echo6 (0.73%), CVA9 (0.36%), CVB3 (0.36%) and co-infections (5.11%). CVA10 and CVA6 had the third and fourth highest prevalence of pathogens for HFMD, respec- tively. The most prevalent season for CVA10 was from April to August, with a peak in April; for CVA6 it was from April to August, with a peak in June. This is the first report of the pathogenic spectrum of en- teroviruses associated with HFMD in Jinan using the GeXP-based multiplex RT-PCR assay. These data will provide the scientific evidence for the prevention and control of epidemics, as well as therapy for HFMD patients.
authors.affiliation: Jinan Municipal Center for Disease Control and Prevention, Jinan, China.
authors.forename: Heng-Yun
authors.initials: HY
authors.lastname: Guan
authors.forename: Meng-Jie
authors.initials: MJ
authors.lastname: Yang
authors.forename: Lan-Zheng
authors.initials: LZ
authors.lastname: Liu
authors.forename: Ji
authors.initials: J
authors.lastname: Wang
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Yang
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Ma
authors.forename: Chun-Rong
authors.initials: CR
authors.lastname: Wang
mesh_terms: D002648:Child; D002675:Child, Preschool; D002681:China; D004770:Enterovirus / Q000235:genetics / Q000472:pathogenicity*; D006232:Hand, Foot and Mouth Disease / Q000821:virology*; D006801:Humans; D007223:Infant; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013997:Time Factors