title: Identification and functional characterization of novel feline cytochrome P450 2A.
abstract: 1. Cytochrome P450s are the major metabolizing enzymes for xenobiotics in humans and other mammals. Although the domestic cat Felis catus, an obligate carnivore, is the most common companion animal, the properties of cytochrome P450 subfamilies are largely unknown. 2. We newly identified the feline CYP2A13, which consists of 494 deduced amino acids, showing the highest identity to CYP2As of dogs, followed by those of pigs, cattle and humans. 3. The feline CYP2A13 transcript and protein were expressed almost exclusively in the liver without particular sex-dependent differences. 4. The feline CYP2A13 protein heterogeneously expressed in Escherichia coli showed metabolic activity similar to those of human and canine CYP2As for coumarin, 7-ethoxycoumarin and nicotine. 5. The results indicate the importance of CYP2A13 in systemic metabolism of xenobiotics in cats.
authors.affiliation: School of Veterinary Medicine, Rakuno Gakuen University , Ebetsu, Hokkaido , Japan .
authors.forename: Gaku
authors.initials: G
authors.lastname: Okamatsu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Komatsu
authors.forename: Akira
authors.initials: A
authors.lastname: Kubota
authors.forename: Takenori
authors.initials: T
authors.lastname: Onaga
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Uchide
authors.forename: Daiji
authors.initials: D
authors.lastname: Endo
authors.forename: Rikio
authors.initials: R
authors.lastname: Kirisawa
authors.forename: Guojun
authors.initials: G
authors.lastname: Yin
authors.forename: Hiroki
authors.initials: H
authors.lastname: Inoue
authors.forename: Takio
authors.initials: T
authors.lastname: Kitazawa
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Uno
authors.forename: Hiroki
authors.initials: H
authors.lastname: Teraoka
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis* / Q000235:genetics; D002415:Cats; D002417:Cattle; D003374:Coumarins / Q000493:pharmacokinetics / Q000494:pharmacology; D004285:Dogs; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D009538:Nicotine / Q000493:pharmacokinetics / Q000494:pharmacology; D009928:Organ Specificity / Q000187:drug effects / Q000502:physiology; D013552:Swine
chemical_list: D003374:Coumarins; C017299:7-ethoxycoumarin; D009538:Nicotine; D001189:Aryl Hydrocarbon Hydroxylases
keywords: Carnivore; cytochrome P450; domestic cats; drug metabolism