title: Infection of the upper respiratory tract of hamsters by the bovine parainfluenza virus type 3 BN-1 strain expressing enhanced green fluorescent protein.
abstract: Bovine parainfluenza virus type 3 (BPIV3) is an important pathogen associated with bovine respiratory disease complex (BRDC). We have generated a recombinant BPIV3 expressing enhanced green fluorescent protein (rBPIV3-EGFP) based on the BN-1 strain isolated in Japan. After intranasal infection of hamsters with rBPIV3-EGFP, EGFP fluorescence was detected in the upper respiratory tract including the nasal turbinates, pharynx, larynx, and trachea. In the nasal turbinates, rBPIV3-EGFP attained high titers (>10(6) TCID50/g of tissue) 2-4 days after infection. Ciliated epithelial cells in the nasal turbinates and trachea were infected with rBPIV3-EGFP. Histopathological analysis indicated that mucosal epithelial cells in bronchi were shed by 6 days after infection, leaving non-ciliated cells, which may have increased susceptibility to bacterial infection leading to the development of BRDC. These data indicate that rBPIV3-EGFP infection of hamsters is a useful small animal model for studying the development of BPIV3-associated BRDC.
authors.affiliation: Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan. Electronic address: ktakeuch@md.tsukuba.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ohkura
authors.affiliation: Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Moeko
authors.initials: M
authors.lastname: Minakuchi
authors.affiliation: Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Sagai
authors.affiliation: Biologics Production, Center for Animal Disease Control and Prevention, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Kokuho
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Misako
authors.initials: M
authors.lastname: Konishi
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
authors.forename: Ken-Ichiro
authors.initials: KI
authors.lastname: Kameyama
authors.affiliation: Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D006224:Cricetinae / Q000821:virology; D004195:Disease Models, Animal; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D029101:Parainfluenza Virus 3, Bovine / Q000235:genetics* / Q000502:physiology; D012141:Respiratory Tract Infections / Q000662:veterinary* / Q000821:virology; D010253:Respirovirus Infections / Q000662:veterinary* / Q000821:virology; D014779:Virus Replication
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Bovine respiratory disease complex; Ciliated epithelial cell; GFP; Hamster; Parainfluenza virus; Pathogenesis; Respiratory tract; Reverse genetics