title: PRFM enhance wound healing process in skin graft.
abstract: Facial plastic and reconstructive surgery often used skin graft on defects that cannot be covered primarily by a local flap. However, wound healing using skin graft is slow, most of the time the graft is contractured and the take of graft is not optimal. Platelet rich fibrin matrix (PRFM) is a new generation of concentrated platelets that produce natural fibrin and reported to speed up the healing process. Application of PRFM in the skin graft implants is expected to increase the survival of the graft. We used porcine as animal models to elucidate the effect of autologous PRFM on wound healing in full-thickness (FTSG) and split-thickness (STSG) skin grafts. Survival level of the skin graft was determined by using ImageJ software based on the formation of collagen type 1 and graft take. We observed that the use of PRFM in FTSG and STSG increased type 1 collagen formation. We also found that PRFM addition in STSG gave the best skin graft take.
authors.affiliation: Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
authors.forename: Mirta
authors.initials: M
authors.lastname: Reksodiputro
authors.affiliation: Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
authors.forename: Dini
authors.initials: D
authors.lastname: Widodo
authors.affiliation: Department of ENT, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
authors.forename: Jenny
authors.initials: J
authors.lastname: Bashiruddin
authors.affiliation: Department of Pathology Anatomy, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia.
authors.forename: Nurjati
authors.initials: N
authors.lastname: Siregar
authors.affiliation: Department of Biology Molecular, Eijkman Institute, Jakarta, Indonesia.
authors.forename: Safarina
authors.initials: S
authors.lastname: Malik
mesh_terms: D000818:Animals; D024042:Collagen Type I / Q000096:biosynthesis; D005260:Female; D005337:Fibrin; D006085:Graft Survival; D006801:Humans; D008297:Male; D053657:Platelet-Rich Plasma; D016038:Skin Transplantation / Q000379:methods; D013552:Swine; D014945:Wound Healing
chemical_list: D024042:Collagen Type I; D005337:Fibrin