title: Invariance and plasticity in the Drosophila melanogaster metabolomic network in response to temperature.
abstract: BACKGROUND
  Metabolomic responses to extreme thermal stress have recently been investigated in Drosophila melanogaster. However, a network level understanding of metabolomic responses to longer and less drastic temperature changes, which more closely reflect variation in natural ambient temperatures experienced during development and adulthood, is currently lacking. Here we use high-resolution, non-targeted metabolomics to dissect metabolomic changes in D. melanogaster elicited by moderately cool (18°C) or warm (27°C) developmental and adult temperature exposures.
  
  
  RESULTS
  We find that temperature at which larvae are reared has a dramatic effect on metabolomic network structure measured in adults. Using network analysis, we are able to identify modules that are highly differentially expressed in response to changing developmental temperature, as well as modules whose correlation structure is strongly preserved across temperature.
  
  
  CONCLUSIONS
  Our results suggest that the effect of temperature on the metabolome provides an easily studied and powerful model for understanding the forces that influence invariance and plasticity in biological networks.
authors.affiliation: Department of Pathology, University of Washington, Box 357705, Seattle, WA, 98195, USA. rmhariharan123@gmail.com.|Laboratory for Integrated Bioinformatics, RIKEN Center for Integrative Medical Sciences, Yokohama, Kanagawa, 230-0045, Japan. rmhariharan123@gmail.com.
authors.forename: Ramkumar
authors.initials: R
authors.lastname: Hariharan
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA, 30602, USA. jmhoffm@uga.edu.
authors.forename: Jessica M
authors.initials: JM
authors.lastname: Hoffman
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA, 30602, USA. thomasa@wusm.wustl.edu.|Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, MO, 63108, USA. thomasa@wusm.wustl.edu.
authors.forename: Ariel S
authors.initials: AS
authors.lastname: Thomas
authors.affiliation: Division of Pulmonary Allergy & Critical Care Medicine, Emory University, Atlanta, GA, 30322, USA. quinlyn.soltow@clinmet.com.|Department of Medicine, Clinical Biomarkers Laboratory, Emory University, Atlanta, GA, 30322, USA. quinlyn.soltow@clinmet.com.|ClinMet Inc, 3210 Merryfield Row, San Diego, CA, 92121, USA. quinlyn.soltow@clinmet.com.
authors.forename: Quinlyn A
authors.initials: QA
authors.lastname: Soltow
authors.affiliation: Division of Pulmonary Allergy & Critical Care Medicine, Emory University, Atlanta, GA, 30322, USA. dpjones@emory.edu.|Department of Medicine, Clinical Biomarkers Laboratory, Emory University, Atlanta, GA, 30322, USA. dpjones@emory.edu.
authors.forename: Dean P
authors.initials: DP
authors.lastname: Jones
authors.affiliation: Department of Pathology, University of Washington, Box 357705, Seattle, WA, 98195, USA. promislo@uw.edu.|Department of Biology, University of Washington, Seattle, WA, 98195, USA. promislo@uw.edu.
authors.forename: Daniel E L
authors.initials: DE
authors.lastname: Promislow
mesh_terms: D000367:Age Factors; D000818:Animals; D004331:Drosophila melanogaster / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D055442:Metabolome / Q000235:genetics / Q000502:physiology*; D055432:Metabolomics / Q000379:methods*; D008954:Models, Biological; D013696:Temperature