title: Chemical Genetics Approach Reveals Importance of cAMP and MAP Kinase Signaling to Lipid and Carotenoid Biosynthesis in Microalgae.
abstract: In this study, we attempted to understand signaling pathways behind lipid biosynthesis by employing a chemical genetics approach based on small molecule inhibitors. Specific signaling inhibitors of MAP kinase or modulators of cAMP signaling were selected to evaluate the functional roles of each of the key signaling pathways in three different microalgal species: Chlamydomonas reinhardtii, Chlorella vulgaris, and Haematococcus pluvialis. Our results clearly indicate that cAMP signaling pathways are indeed positively associated with microalgal lipid biosynthesis. In contrast, MAP kinase pathways in three microalgal species are all negatively implicated in both lipid and carotenoid biosynthesis.
authors.affiliation: Division of Environmental Science and Ecological Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, Republic of Korea.
authors.forename: Yoon-E
authors.initials: YE
authors.lastname: Choi
authors.affiliation: Department of Food Science and Engineering, Ewha Womans University, Seoul, 120-750, Republic of Korea.
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Rhee
authors.affiliation: Department of Food Science and Technology, Jungwon University, Chungbuk 367-805, Republic of Korea.
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeonbuk 580-185, Republic of Korea.
authors.forename: Joon-Woo
authors.initials: JW
authors.lastname: Ahn
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Hwang
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Daejeon 305-701, Republic of Korea.|Advanced Biomass R&D Center, KAIST, Daejeon 305-701, Republic of Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D002338:Carotenoids / Q000032:analysis / Q000096:biosynthesis*; D016825:Chlamydomonas reinhardtii / Q000737:chemistry* / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D008055:Lipids; D017382:Reactive Oxygen Species; D002338:Carotenoids; D000242:Cyclic AMP; D020928:Mitogen-Activated Protein Kinases; D005947:Glucose