title: Indirect competitive assays on DVD for direct multiplex detection of drugs of abuse in oral fluids.
abstract: On-site oral fluid testing for drugs of abuse has become prominent in order to take immediate administrative action in an enforcement process. Herein, we report a DVD technology-based indirect competitive immunoassay platform for the quantitative detection of drugs of abuse. A microfluidic approach was adapted to prepare multiplex immunoassays on a standard DVD-R, an unmodified multimode DVD/Blu-Ray drive to read signal, and a free disc-quality analysis software program to process the data. The DVD assay platform was successfully demonstrated for the simultaneous, quantitative detection of drug candidates (morphine and cocaine) in oral fluids with high selectivity. The detection limit achieved was as low as 1.0 ppb for morphine and 5.0 ppb for cocaine, comparable with that of standard mass spectrometry and ELISA methods.
authors.affiliation: Department of Chemistry, Beijing Normal University , Beijing 100875, P. R. China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Li
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Shi
authors.forename: Hua-Zhong
authors.initials: HZ
authors.lastname: Yu
mesh_terms: D003042:Cocaine / Q000032:analysis*; D018765:Dopamine Uptake Inhibitors / Q000032:analysis*; D004867:Equipment Design; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation; D009020:Morphine / Q000032:analysis*; D009294:Narcotics / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000295:instrumentation*
chemical_list: D018765:Dopamine Uptake Inhibitors; D013287:Illicit Drugs; D009294:Narcotics; D009020:Morphine; D003042:Cocaine