title: Antioxidant potential of date (Phoenix dactylifera L.) seed protein hydrolysates and carnosine in food and biological systems.
abstract: Date seed protein hydrolysates were evaluated for antioxidant activity as well as solubility and water-holding capacity in food and biological model systems. Date seed protein hydrolysates as well as carnosine exhibited >80% of solubility over a pH range of 2-12. The hydrolysates and carnosine at 0.5% (w/w) were also found to be effective in enhancing water-holding capacity and cooking yield in a fish model system, which was nearly similar to sodium tripolyphosphate (STPP; 0.3%, w/w). Incorporation of hydrolysates (200 ppm) in fish model systems resulted in the highest inhibition (30%) of oxidation in comparison to butylated hydroxytoluene (BHT; 9%). In addition, hydrolysates and carnosine inhibited β-carotene oxidation by 75%. The hydrolysates (0.1 mg/mL) inhibited LDL cholesterol oxidation by 60%, whereas carnosine inhibited oxidation by 80% after 12 h of incubation. Additionally, hydrolysates and carnosine effectively inhibited hydroxyl (6 mg/mL) and peroxyl (0.1 mg/mL) radical-induced DNA scission. Therefore, date seed protein hydrolysates could be used as a potential functional food ingredient for health promotion.
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland , St. John's, Newfoundland, Canada A1B 3X9.
authors.forename: Priyatharini
authors.initials: P
authors.lastname: Ambigaipalan
authors.forename: Fereidoon
authors.initials: F
authors.lastname: Shahidi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D002336:Carnosine / Q000737:chemistry / Q000494:pharmacology*; D004278:DNA, Superhelical / Q000187:drug effects; D005399:Fishes; D005502:Food; D055951:Functional Food; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000737:chemistry; D065749:Phoeniceae; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000494:pharmacology; D017747:Seafood; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004278:DNA, Superhelical; D008077:Lipoproteins, LDL; D010940:Plant Proteins; D011492:Protein Hydrolysates; D014867:Water; D002336:Carnosine
keywords: DNA scission; LDL cholesterol; carnosine; date seed; protein hydrolysate; solubility; water-holding capacity