title: [Alternative polyadenylation site analysis of tumor-related genes based on 3'RACE in gastric cancer cells].
abstract: OBJECTIVE
  To analyze the alteration in alternative polyadenylation (APA) sites of tumor-related genes in gastric cancer cells.
  
  
  METHODS
  We used 3'RACE to capture the APA sites of two tumor-related genes (HSP90α and SEC11A) in gastric cancer cell lines MKN45, MKN28 and AGS, and compared the results with annotated poly(A) sites in UCSC database.
  
  
  RESULTS
  We found new APA sites in the two tumor-related genes in gastric cancer cells to produce new mRNA isoforms with different 3'UTRs.
  
  
  CONCLUSIONS
  There are new mRNA isoforms of HSP90α and SEC11A derived from ATA in gastric cancer cells, which provides new insights into the mechanisms of gastric tumorigenesis.
authors.affiliation: Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine of Ministry of Education, Shanghai Jiaotong University, Shanghai 200240, China.E-mail: dengpanlai@gmail.com.
authors.forename: Dengpan
authors.initials: D
authors.lastname: Lai
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Yani
authors.initials: Y
authors.lastname: Kang
mesh_terms: D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D052138:Genes, Neoplasm; D006801:Humans; D026723:Polyadenylation; D013274:Stomach Neoplasms / Q000235:genetics*