title: SHARPIN overexpression induces tumorigenesis in human prostate cancer LNCaP, DU145 and PC-3 cells via NF-κB/ERK/Akt signaling pathway.
abstract: SHARPIN emerges higher expression in prostate cancerous tissues than in benign prostate hyperplasia by means of immunohistochemistry in our previous study. In this work, we performed the gain of function assay and find that overexpression of SHARPIN in LNCaP, DU145 and PC-3 cells promoted cell proliferation, invasiveness and reduced apoptosis. Furthermore, SHARPIN overexpression displayed elevated Bcl-2 and Survivin expression and reduced levels of Bax, cleaved caspase-3. Meanwhile, entropic expression of SHARPIN increased the levels of phosphorylated p65, IkBα, ERK and Akt, were selectively increased in these cells. Collectively, our study unraveled the ability of SHARPIN overexpression to induce tumorigenesis of prostate cancer cells through the NF-kB/ERK/Akt pathway and transformation of apoptosis-associated proteins.
authors.affiliation: Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou, 510120, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.forename: Yiming
authors.initials: Y
authors.lastname: Lai
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Tao
authors.initials: T
authors.lastname: Du
authors.forename: Lexiang
authors.initials: L
authors.lastname: Zeng
authors.forename: Ganping
authors.initials: G
authors.lastname: Wang
authors.forename: Xianju
authors.initials: X
authors.lastname: Chen
authors.forename: Jieqing
authors.initials: J
authors.lastname: Chen
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Yu
authors.forename: Simin
authors.initials: S
authors.lastname: Zhang
authors.forename: Yiming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Guo
mesh_terms: D015153:Blotting, Western; D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D014452:Ubiquitins / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D016328:NF-kappa B; C575183:SHARPIN protein, human; D014452:Ubiquitins; D051057:Proto-Oncogene Proteins c-akt