title: Solena amplexicaulis induces cell cycle arrest, apoptosis and inhibits angiogenesis in hepatocarcinoma cells and HUVECs.
abstract: Solena amplexicaulis (Lam.) Gandhi (SA) has been used as a traditional medicine for the treatment of dysentery, multiple abscess, gastralgia, urethritis, and eczema in the minority area of China. This study was aimed to examine the cell proliferation inhibitory activity of the SA extract (SACE) and its mechanism of action in human hepatoma cell line (HepG2) and evaluate its anti-angiogenesis activity in human umbilical vein endothelial cell line (HUVEC). SACE could inhibit the growth of HepG2 cells in a dose- and time-dependent manner. FCM analysis showed that SACE could induce G2/M phase arrest, cell apoptosis, the mitochondrial membrane potential loss (ΔΨm) and increase the production of intracellular ROS of HepG2 cells. After treatment with SACE, topical morphological changes of apoptotic body formation, obvious increase of apoptosis-related protein expressions, such as Bax, cytochrome c, caspase-3, PARP-1, and decrease of Bcl-2, procaspase-9 protein expressions were observed at the same time. Moreover, SACE caused the significant inhibition of endothelial cell migration and tube formation in HUVEC cells. The results suggested that SACE could act as an angiogenesis inhibitor and induce cell apoptosis via a caspase-dependent mitochondrial pathway. Therefore, SACE could be a potent candidate for the prevention and treatment of liver cancer.
authors.affiliation: School of Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, Jiangsu 213164, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.forename: Yuan Yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: He Fei
authors.initials: HF
authors.lastname: Jiang
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.forename: Qian Hui
authors.initials: QH
authors.lastname: Huang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Kun
authors.initials: K
authors.lastname: Hu
authors.forename: Kun
authors.initials: K
authors.lastname: Wei
mesh_terms: D020533:Angiogenesis Inhibitors; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000098:blood supply* / Q000188:drug therapy; D020169:Caspases / Q000502:physiology; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D019662:Cucurbitaceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000098:blood supply* / Q000188:drug therapy; D051379:Mice; D008928:Mitochondria / Q000502:physiology; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D015398:Signal Transduction / Q000187:drug effects; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts; D020169:Caspases
keywords: Angiogenesis; Anticancer; Apoptosis; Nrf2; ROS; Solena amplexicaulis (Lam.) Gandhi