title: Bacterial community composition in three freshwater reservoirs of different alkalinity and trophic status.
abstract: In order to investigate the factors controlling the bacterial community composition (BCC) in reservoirs, we sampled three freshwater reservoirs with contrasted physical and chemical characteristics and trophic status. The BCC was analysed by 16S rRNA gene amplicon 454 pyrosequencing. In parallel, a complete dataset of environmental parameters and phytoplankton community composition was also collected. BCC in the analysed reservoirs resembled that of epilimnetic waters of natural freshwater lakes with presence of Actinobacteria, Alpha- and Betaproteobacteria, Cytophaga-Flavobacteria-Bacteroidetes (CFB) and Verrucomicrobia groups. Our results evidenced that the retrieved BCC in the analysed reservoirs was strongly influenced by pH, alkalinity and organic carbon content, whereas comparatively little change was observed among layers in stratified conditions.
authors.affiliation: Laboratory of Freshwater Ecology, University of Namur, Namur, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Llirós
authors.affiliation: Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Özgül
authors.initials: Ö
authors.lastname: Inceoğlu
authors.affiliation: Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Tamara
authors.initials: T
authors.lastname: García-Armisen
authors.affiliation: Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Adriana
authors.initials: A
authors.lastname: Anzil
authors.affiliation: Laboratory of Freshwater Ecology, University of Namur, Namur, Belgium.
authors.forename: Bruno
authors.initials: B
authors.lastname: Leporcq
authors.affiliation: Laboratory of Freshwater Ecology, University of Namur, Namur, Belgium.
authors.forename: Lise-Marie
authors.initials: LM
authors.lastname: Pigneur
authors.affiliation: Laboratory of Freshwater Ecology, University of Namur, Namur, Belgium.
authors.forename: Laurent
authors.initials: L
authors.lastname: Viroux
authors.affiliation: Chemical Oceanography Unit, University of Liège, Liège, Belgium.
authors.forename: François
authors.initials: F
authors.lastname: Darchambeau
authors.affiliation: Laboratory of Freshwater Ecology, University of Namur, Namur, Belgium.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Descy
authors.affiliation: Ecologie des Systèmes Aquatiques, Université Libre de Bruxelles, Brussels, Belgium.
authors.forename: Pierre
authors.initials: P
authors.lastname: Servais
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification; D041963:Bacteroidetes / Q000235:genetics / Q000302:isolation & purification; D020562:Betaproteobacteria / Q000235:genetics / Q000302:isolation & purification; D003589:Cytophaga / Q000235:genetics / Q000302:isolation & purification; D041965:Flavobacteriaceae / Q000235:genetics / Q000302:isolation & purification; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D010839:Phytoplankton / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S