title: Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR.
abstract: Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Jesse J
authors.initials: JJ
authors.lastname: Waggoner
authors.affiliation: Department of Pre-Clinical Studies, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Nikita S D
authors.initials: NS
authors.lastname: Sahadeo
authors.affiliation: Trinidad Public Health Laboratory, Port of Spain, Trinidad and Tobago.
authors.forename: Arianne
authors.initials: A
authors.lastname: Brown
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Alisha
authors.initials: A
authors.lastname: Mohamed-Hadley
authors.affiliation: Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
authors.forename: Dexter
authors.initials: D
authors.lastname: Hadley
authors.affiliation: Trinidad Public Health Laboratory, Port of Spain, Trinidad and Tobago.
authors.forename: Leslie
authors.initials: L
authors.lastname: Carrington
authors.affiliation: Department of Pre-Clinical Studies, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Christine V F
authors.initials: CV
authors.lastname: Carrington
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. Electronic address: bpinsky@stanford.edu.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Pinsky
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003715:Dengue / Q000175:diagnosis* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D065288:Serogroup; D014300:Trinidad and Tobago; D055815:Young Adult
keywords: Dengue; Molecular; Pathology