title: In vivo and continuous measurement of bisulfide in the hippocampus of rat's brain by an on-line integrated microdialysis/droplet-based microfluidic system.
abstract: An on-line and continuous approach was demonstrated for in vivo measurement of bisulfide in rat's brain. A modified droplet-based microfluidic system was constructed, which allowed on-line qualification of the fluorescence responses of the gold nanoparticle-glutathione-fluorescein isothiocyanate probe to the variation of bisulfide in the presence of the cerebral microdialysate background. The on-line method achieved a dynamic working range from 5.0 μM to 40 μM and a detection limit of 2.5 μM. The in vivo bisulfide concentration in the hippocampus of rat's brain was measured under different physiological conditions. The on-line method may facilitate the study of H2S biology by providing a previously unattainable continuous record of H2S variation in living animals. It also provides a practical platform for in vivo and continuous monitoring of other important species in cerebral systems.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn.
authors.forename: Feidan
authors.initials: F
authors.lastname: Gu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiaocui
authors.initials: X
authors.lastname: Zhu
authors.forename: Meiping
authors.initials: M
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Lanqun
authors.initials: L
authors.lastname: Mao
mesh_terms: D000818:Animals; D019793:Fluorescein / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D005978:Glutathione / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006624:Hippocampus / Q000378:metabolism*; D017879:Isothiocyanates / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D053768:Metal Nanoparticles / Q000737:chemistry; D017551:Microdialysis / Q000379:methods*; D009862:Online Systems; D051381:Rats; D013440:Sulfides / Q000737:chemistry / Q000378:metabolism*; D018511:Systems Integration; D014851:Wakefulness
chemical_list: D005456:Fluorescent Dyes; D017879:Isothiocyanates; D013440:Sulfides; C037152:isothiocyanic acid; D006046:Gold; D005978:Glutathione; D019793:Fluorescein