title: A genetic association study of activated partial thromboplastin time in European Americans and African Americans: the ARIC Study.
abstract: Reduced activated partial thromboplastin time (aPTT) is a risk marker for incident and recurrent venous thromboembolism (VTE). Genetic factors influencing aPTT are not well understood, especially in populations of non-European ancestry. The present study aimed to identify aPTT-related gene variants in both European Americans (EAs) and African Americans (AAs). We conducted a genetic association study for aPTT in 9719 EAs and 2799 AAs from the Atherosclerosis Risk in Communities (ARIC) study. Using the Candidate Gene Association Resource (CARe) consortium candidate gene array, the analyses were based on ∼50 000 SNPs in ∼2000 candidate genes. In EAs, the analyses identified a new independent association for aPTT in F5 (rs2239852, P-value = 1.9 × 10(-8)), which clusters with a coding variant rs6030 (P-value = 7.8 × 10(-7)). The remaining significant signals were located on F5, HRG, KNG1, F11, F12 and ABO and have been previously reported in EA populations. In AAs, significant signals were identified in KNG1, HRG, F12, ABO and VWF, with the leading variants in KNG1, HRG and F12 being the same as in the EAs; the significant variant in VWF (rs2229446, P-value = 1.2 × 10(-6)) was specific to the AA sample (minor allele frequency = 19% in AAs and 0.2% in EAs) and has not been previously reported. This is the first study to report aPTT-related genetic variants in AAs. Our findings in AAs demonstrate transferability of previously reported associations with KNG1, HRG and F12 in EAs. We also identified new associations at F5 in EAs and VWF in AAs that have not been previously reported for aPTT.
authors.affiliation: Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, MN 55454, USA.
authors.forename: Lu-Chen
authors.initials: LC
authors.lastname: Weng
authors.affiliation: Departments of Medicine and Pathology, University of Vermont, Burlington, VT 05405, USA.
authors.forename: Mary
authors.initials: M
authors.lastname: Cushman
authors.affiliation: Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, MN 55454, USA.
authors.forename: James S
authors.initials: JS
authors.lastname: Pankow
authors.affiliation: Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA and.
authors.forename: Saonli
authors.initials: S
authors.lastname: Basu
authors.affiliation: Human Genetics Center and Institute of Molecular Medicine, University of Texas Health Science Center at Houston, Houston, TX 77030, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Boerwinkle
authors.affiliation: Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, MN 55454, USA.
authors.forename: Aaron R
authors.initials: AR
authors.lastname: Folsom
authors.affiliation: Division of Epidemiology and Community Health, School of Public Health, University of Minnesota, Minneapolis, MN 55454, USA, tang0097@umn.edu.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tang
mesh_terms: D001741:Black or African American / Q000235:genetics*; D000368:Aged; D050197:Atherosclerosis / Q000235:genetics* / Q000503:physiopathology; D005260:Female; D056726:Genetic Association Studies; D055106:Genome-Wide Association Study; D006801:Humans; D007704:Kininogens / Q000235:genetics; D008297:Male; D008875:Middle Aged; D039842:Neural Cell Adhesion Molecule L1 / Q000235:genetics; D010314:Partial Thromboplastin Time; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D011506:Proteins / Q000235:genetics; D044465:White People / Q000235:genetics*; D000099053:Histidine-Rich Glycoprotein
chemical_list: C000591034:KNG1 protein, human; D007704:Kininogens; D039842:Neural Cell Adhesion Molecule L1; D011506:Proteins; D000099053:Histidine-Rich Glycoprotein