title: Identification of protein network alterations upon retinal ischemia-reperfusion injury by quantitative proteomics using a Rattus norvegicus model.
abstract: Retinal ischemia is a common feature associated with several ocular diseases, including diabetic retinopathy. In this study, we investigated the effect of a retinal ischemia and reperfusion (I/R) injury on protein levels via a quantitative shotgun strategy using stable isotope dimethyl labeling combined with LC-MS/MS analysis. Based on the relative quantitation data of 1088 proteins, 234 proteins showed a greater than 1.5-fold change following I/R injury, 194 of which were up-regulated and 40 were down-regulated. Gene ontology analysis revealed that after I/R injury, there was an increase in the metabolic-process related proteins but a decline in cell communication, system process and transport-related proteins. A ribosome protein network and a secreted protein network consisting of many protease inhibitors were identified among the up-regulated proteins, despite a suppression of the mammalian target of rapamycin (mTOR) pathway following the I/R injury. A synaptic-related protein network was found to be significantly down-regulated, implicating a functional reduction of neurons following a retinal I/R injury. Our results provide new systems-biology clues for the study of retinal ischemia.
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Han
authors.initials: H
authors.lastname: Tian
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Leilei
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Cai
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Zheng
authors.affiliation: College of Life Sciences, Wuhan University, Wuhan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Guo
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D004195:Disease Models, Animal; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006801:Humans; D008297:Male; D040901:Proteomics / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D012164:Retinal Diseases / Q000378:metabolism*; D015398:Signal Transduction; D049490:Systems Biology / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods