title: Production of cellulolytic enzymes by Pleurotus species on lignocellulosic wastes using novel pretreatments.
abstract: In the present investigation three species of Pleurotus i.e. P. sajor&mdash;caju (P1), P. florida (P2) and P. flabellatus (P3) along with two lignocellulosic substrates namely paddy straw and wheat straw were selected for evaluation of production of extracellular cellulolytic enzymes. During the cultivation of three species of Pleurotus under in vivo condition, the two lignocellulosic substrates were treated with plants extracts (aqueous extracts of ashoka leaves (A) and neem oil (B)), hot water (H) and chemicals (C).Among all treatments, neem oil treated substrates supported better enzyme production followed by aqueous extract of ashoka leaves, hot water and chemical treatment. Between the two substrates paddy straw supported better enzyme production than wheat straw. P. flabellatus showed maximum activity of exoglucanase, endoglucanase and &#946;&mdash;glucosidase followed by P. florida and P. sajor&mdash;caju.
authors.affiliation: University of Allahabad Centre of Biotechnology Allahabad India mpsingh.16&commat;gmail.com.
authors.forename: M P
authors.initials: MP
authors.lastname: Singh
authors.affiliation: V.B.S. Purvanchal University Department of Biotechnology Jaunpur India.
authors.forename: A K
authors.initials: AK
authors.lastname: Pandey
authors.affiliation: V.B.S. Purvanchal University Department of Biotechnology Jaunpur India.
authors.forename: S K
authors.initials: SK
authors.lastname: Vishwakarma
authors.affiliation: V.B.S. Purvanchal University Department of Biotechnology Jaunpur India.
authors.forename: A K
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: V.B.S. Purvanchal University Department of Environmental Science Jaunpur India.
authors.forename: V K
authors.initials: VK
authors.lastname: Pandey
authors.affiliation: BHU Department of Zoology Varanasi India.
authors.forename: V K
authors.initials: VK
authors.lastname: Singh
mesh_terms: D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism; D044643:Glucan 1,4-beta-Glucosidase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology* / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: C036909:lignocellulose; D008031:Lignin; D001617:beta-Glucosidase; D002480:Cellulase; D044643:Glucan 1,4-beta-Glucosidase