title: The EPIYA-ABCC motif pattern in CagA of Helicobacter pylori is associated with peptic ulcer and gastric cancer in Mexican population.
abstract: BACKGROUND
  Helicobacter pylori chronic infection is associated with chronic gastritis, peptic ulcer, and gastric cancer. Cytotoxin-associated gene A (cagA)-positive H. pylori strains increase the risk of gastric pathology. The carcinogenic potential of CagA is linked to its polymorphic EPIYA motif variants. The goals of this study were to investigate the frequency of cagA-positive Helicobacter pylori in Mexican patients with gastric pathologies and to assess the association of cagA EPIYA motif patterns with peptic ulcer and gastric cancer.
  
  
  METHODS
  A total of 499 patients were studied; of these, 402 had chronic gastritis, 77 had peptic ulcer, and 20 had gastric cancer. H. pylori DNA, cagA, and the EPIYA motifs were detected in total DNA from gastric biopsies by PCR. The type and number of EPIYA segments were determined by the electrophoretic patterns. To confirm the PCR results, 20 amplicons of the cagA 3' variable region were sequenced, and analyzed in silico, and the amino acid sequence was predicted with MEGA software, version 5. The odds ratio (OR) was calculated to determine the associations between the EPIYA motif type and gastric pathology and between the number of EPIYA-C segments and peptic ulcers and gastric cancer.
  
  
  RESULTS
  H. pylori DNA was found in 287 (57.5%) of the 499 patients, and 214 (74%) of these patients were cagA-positive. The frequency of cagA-positive H. pylori was 74.6% (164/220) in chronic gastritis patients, 73.6% (39/53) in peptic ulcer patients, and 78.6% (11/14) in gastric cancer patients. The EPIYA-ABC pattern was more frequently observed in chronic gastritis patients (79.3%, 130/164), while the EPIYA-ABCC sequence was more frequently observed in peptic ulcer (64.1%, 25/39) and gastric cancer patients (54.5%, 6/11). However, the risks of peptic ulcer (OR = 7.0, 95% CI = 3.3-15.1; p < 0.001) and gastric cancer (OR = 5.9, 95% CI = 1.5-22.1) were significantly increased in individuals who harbored the EPIYA-ABCC cagA gene pattern.
  
  
  CONCLUSIONS
  cagA-positive H. pylori is highly prevalent in southern Mexico, and all CagA variants were of the western type. The cagA alleles that code for EPIYA-ABCC motif patterns are associated with peptic ulcers and gastric cancer.
authors.forename: Fredy Omar
authors.initials: FO
authors.lastname: Beltrán-Anaya
authors.forename: Tomás Manuel
authors.initials: TM
authors.lastname: Poblete
authors.forename: Adolfo
authors.initials: A
authors.lastname: Román-Román
authors.forename: Salomón
authors.initials: S
authors.lastname: Reyes
authors.forename: José
authors.initials: J
authors.lastname: de Sampedro
authors.forename: Oscar
authors.initials: O
authors.lastname: Peralta-Zaragoza
authors.forename: Miguel Ángel
authors.initials: MÁ
authors.lastname: Rodríguez
authors.forename: Oscar
authors.initials: O
authors.lastname: del Moral-Hernández
authors.forename: Berenice
authors.initials: B
authors.lastname: Illades-Aguiar
authors.forename: Gloria
authors.initials: G
authors.lastname: Fernández-Tilapa
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002908:Chronic Disease; D005260:Female; D005756:Gastritis / Q000235:genetics / Q000382:microbiology; D016481:Helicobacter Infections / Q000235:genetics / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D059372:Nucleotide Motifs; D010437:Peptic Ulcer / Q000235:genetics / Q000382:microbiology*; D020539:Sequence Analysis, Protein; D013274:Stomach Neoplasms / Q000235:genetics / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori