title: Construction of biocompatible porous tissue scaffold from the decellularized umbilical artery.
abstract: The scaffolds prepared from the tissue decellularization conserve the porous 3-D structure and provide an optimal matrix for the tissue regeneration. Since decade, the enzymatic digestion, chemical reagent treatment and mechanical actions such as eversion and abrasion have been used to remove the cells from the intact matrix. In this study, we optimized an enzymatic method to decellularize the umbilical artery to construct a 3-D porous scaffold which is suitable for the culture of mesenchymal stem cells (MSCs). The scaffold maintained the interconnected porous structure. It remained the similar high water content 95.3 ± 1% compared to 94.9 ± 0.6% in the intact umbilical artery (p>0.05). The decellularization process decreased the stress from 0.24 ± 0.05 mPa to 0.15 ± 0.06 mPa (p<0.05). However the decellularization did not change the strain of the artery (45 ± 15% vs. 53 ± 10%, p>0.05). When the scaffold was transplanted to the subcutaneous tissue in the wild type mice, there were less T cells appeared in the surrounding tissue which meant the decreased the immunogenicity by decellularization. This scaffold also supported the adhesion and proliferation of the MSCs. In this study, we constructed a biological compatible porous scaffold from the decellularized umbilical artery which may provide a suitable scaffold for cell-matrix interaction studies and for tissue engineering.
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Wu
authors.affiliation: Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Man
authors.initials: M
authors.lastname: Wu
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: CNRS - UMR UL 7365 IMoPA, Faculté de Médecine, Vandœuvre-lès-Nancy, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Velot
authors.affiliation: CHU, Unité de Thérapie Cellulaire, Vandœuvre-lès-Nancy, France CNRS - UMR UL 7365 IMoPA, Faculté de Médecine, Vandœuvre-lès-Nancy, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Decot
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cui
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Huang
authors.affiliation: CHU, Unité de Thérapie Cellulaire, Vandœuvre-lès-Nancy, France CNRS - UMR UL 7365 IMoPA, Faculté de Médecine, Vandœuvre-lès-Nancy, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Stoltz
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.affiliation: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002474:Cell-Free System; D002478:Cells, Cultured; D019245:Compressive Strength; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D051379:Mice; D016062:Porosity; D013718:Tensile Strength; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds; D014469:Umbilical Arteries / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials
keywords: Decellularization; biological scaffold; mesenchymal stem cells; tissue engineering; umbilical artery