title: [Characterization of the whole genome from a human parechovirus type 3 detected from the serum of a child with sepsis in Beijing, China].
abstract: Human parechovirus type 3 (HPeV3) is an important pathogen of severe sepsis. HPeV3 is a non- enveloped, single-stranded, positive-sense RNA virus with a linear and continuous genomic RNA. The complete genome of a HPeV3 (BJ-C3174) strain was analyzed from the serum specimen from a child with sepsis hospitalized in Beijing, China, in 2012. The whole genome of BJ-C3174 was 7329 nucleotides (nt) in length excluding a poly (A) tail. One large open reading frame (ORF) of 6531 nt encoding a putative polyprotein precursor of 2177 amino acids (aa) was flanked by a 5' untranslated region (UTR) of 709 nt and 3' UTR of 91 nt. Phylogenetic analysis showed that BJ-C3174 belonged to HPeV3 and was closest to the HPeV3 strain BONN-2 from Germany. Compared with HPeV1-8 reference strains, BJ-C3174 shared the highest similarities with BONN-2 in full length and in each of the gene segments of the genome. The nucleotide and predicted amino acid identities of the whole genome between BJ-C3174 and BONN-2 were 99.3% and 99.8%, respectively, which were higher than those compared with HPeV3 prototype. Recom- bination of the gene segment with other HPeVs types was not identified.
authors.affiliation: Capital Institute of pediatrics, Beijing Key Laboratory of Etiology of Viral Diseases in Children, China.
authors.forename: Ru-Nan
authors.initials: RN
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Qian
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Deng
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Qin-Wei
authors.initials: QW
authors.lastname: Song
authors.forename: Ya-Xin
authors.initials: YX
authors.lastname: Ding
mesh_terms: D000595:Amino Acid Sequence; D002648:Child; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D030061:Parechovirus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D018805:Sepsis / Q000097:blood / Q000821:virology*