title: A Substrate Fed-Batch Biphasic Catalysis Process for the Production of Natural Crosslinking Agent Genipin with Fusarium solani ACCC 36223.
abstract: The natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. Because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. In this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. A 10 L ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying genipin yield. With Fusarium solani ACCC 36223, 15.7 g/l genipin in the ethyl acetate phase was obtained, corresponding to space-time yields of 0.654 g l(-1) h(-1).
authors.affiliation: Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, P.R. China.
authors.forename: Yuyao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, P.R. China.
authors.forename: Botao
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, P.R. China.
authors.forename: Xiaode
authors.initials: X
authors.lastname: Huang
authors.affiliation: Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Nanjing Research Institute for Comprehensive Utilization of Wild Plants, Nanjing 210042, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Qian
mesh_terms: D061249:Batch Cell Culture Techniques; D003432:Cross-Linking Reagents / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005670:Fusarium / Q000254:growth & development / Q000378:metabolism*; D006868:Hydrolysis; D039823:Iridoids / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D003470:Culture Media; D039823:Iridoids; C007835:geniposide; C007834:genipin