title: The influence of different factors including fnbA and mecA expression on biofilm formed by MRSA clinical isolates with different genetic backgrounds.
abstract: Biofilm formation is considered an important virulence factor in implanted device-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Recent studies demonstrated that the ica-independent biofilms produced by MRSA are multifactorial. Despite the recent progress achieved in this field, the bacterial factors associated with biofilm formation/accumulation and regulation among clinical MRSA isolates remain largely unknown. In this study, using MRSA isolates from diverse multilocus sequence typing (MLST) clonal complexes that produce different amounts of biofilm, and a number of phenotypic and molecular approaches, we investigated the correlation between biofilm-associated factors and the ability of the bacteria to accumulate biofilm.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil. Electronic address: marinafarrel@yahoo.com.br.
authors.forename: Marina Farrel
authors.initials: MF
authors.lastname: Côrtes
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil. Electronic address: crisbeltrame23@gmail.com.
authors.forename: Cristiana Ossaille
authors.initials: CO
authors.lastname: Beltrame
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil. Electronic address: marianasevero@gmail.com.
authors.forename: Mariana Severo
authors.initials: MS
authors.lastname: Ramundo
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil. Electronic address: fabienneaf@micro.ufrj.
authors.forename: Fabienne Antunes
authors.initials: FA
authors.lastname: Ferreira
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Centro de Ciências da Saúde, 21941.902 Rio de Janeiro, RJ, Brazil. Electronic address: agnes@micro.ufrj.br.
authors.forename: Agnes Marie Sá
authors.initials: AM
authors.lastname: Figueiredo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D008807:Mice, Inbred BALB C; D016459:Prosthesis-Related Infections / Q000382:microbiology; D013203:Staphylococcal Infections / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors
keywords: Biofilm; DNase; Methicillin-resistant Staphylococcus aureus; Protease; agr; fnbA; pbp2a; sarA