title: Genome-based proteomic analysis of Lignosus rhinocerotis (Cooke) Ryvarden sclerotium.
abstract: Lignosus rhinocerotis (Cooke) Ryvarden (Polyporales, Basidiomycota), also known as the tiger milk mushroom, has received much interest in recent years owing to its wide-range ethnobotanical uses and the recent success in its domestication. The sclerotium is the part with medicinal value. Using two-dimensional gel electrophoresis coupled with mass spectrometry analysis, a total of 16 non-redundant, major proteins were identified with high confidence level in L. rhinocerotis sclerotium based on its genome as custom mapping database. Some of these proteins, such as the putative lectins, immunomodulatory proteins, superoxide dismutase, and aegerolysin may have pharmaceutical potential; while others are involved in nutrient mobilization and the protective antioxidant mechanism in the sclerotium. The findings from this study provide a molecular basis for future research on potential pharmacologically active proteins of L. rhinocerotis.
authors.affiliation: 1. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;
authors.forename: Hui-Yeng Yeannie
authors.initials: HY
authors.lastname: Yap
authors.affiliation: 1. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;
authors.forename: Shin-Yee
authors.initials: SY
authors.lastname: Fung
authors.affiliation: 2. Ligno Biotech Sdn. Bhd., 43300 Balakong Jaya, Selangor, Malaysia.
authors.forename: Szu-Ting
authors.initials: ST
authors.lastname: Ng
authors.affiliation: 2. Ligno Biotech Sdn. Bhd., 43300 Balakong Jaya, Selangor, Malaysia.
authors.forename: Chon-Seng
authors.initials: CS
authors.lastname: Tan
authors.affiliation: 1. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia;
authors.forename: Nget-Hong
authors.initials: NH
authors.lastname: Tan
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D006460:Hemolysin Proteins / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D016366:Open Reading Frames; D010946:Plants, Medicinal / Q000737:chemistry; D011124:Polyporaceae / Q000737:chemistry* / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000975:Antioxidants; D005656:Fungal Proteins; D006460:Hemolysin Proteins; C461279:aegerolysin
keywords: LC-MS; Lignosus rhinocerotis; MALDI-MS; proteins.; proteomic analysis