title: Analysis of serum cytokine levels in larynx squamous cell carcinoma and dysplasia patients.
abstract: BACKGROUND
  Although the imbalance of cytokines in Head and Neck Squamous Cell Carcinoma (HNSCC) is well known, there is scarce data regarding its occurrence during dysplasia, before the malignant transformation.
  
  
  OBJECTIVE
  To determine whether laryngeal dysplasia patients show a different cytokine profile than patients with cancer and healthy controls.
  
  
  METHODS
  Seventeen newly diagnosed, untreated larynx squamous cell carcinoma (SCC) and six laryngeal dysplasia patients as well as 22 healthy controls were analyzed for circulating cytokines. A flowcytometry Th1/Th2 cytokine array kit was used to quantitatively measure Interleukin-2 (IL-2), IL-4, IL-6, IL-10, Tumor Necrosis Factor-α (TNF-α) and Interferon-γ (IFN-γ) levels. Additionally, IL-8 levels were determined through ELISA.
  
  
  RESULTS
  IL-6, IL-8 and IL-10 were determined to be statistically increased in SCC patients (p<0.05). IL-8 and IL-10 levels were also higher in SCC patients than dysplasia patients (p<0.05). Additionally, IL-6 and IL-10 were all found to be markedly increased in dysplasia patients compared with controls (p<0.05).
  
  
  CONCLUSIONS
  Our results demonstrate an imbalance of IL-6 and IL-10 not only in HNSCC but also in laryngeal dysplasia.
authors.affiliation: Akdeniz University, Medical Faculty, Department of Medical Microbiology, Antalya, Turkey, e-mail: metgor@ttmail.com.
authors.forename: Mete
authors.initials: M
authors.lastname: Eyigor
authors.forename: Hulya
authors.initials: H
authors.lastname: Eyigor
authors.forename: Ustun
authors.initials: U
authors.lastname: Osma
authors.forename: Mustafa Deniz
authors.initials: MD
authors.lastname: Yilmaz
authors.forename: Nuray
authors.initials: N
authors.lastname: Erin
authors.forename: Omer Tarik
authors.initials: OT
authors.lastname: Selcuk
authors.forename: Cem
authors.initials: C
authors.lastname: Sezer
authors.forename: Meral
authors.initials: M
authors.lastname: Gultekin
authors.forename: Sadi
authors.initials: S
authors.lastname: Koksoy
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007818:Laryngeal Diseases / Q000276:immunology*; D007822:Laryngeal Neoplasms / Q000276:immunology*; D007830:Larynx / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D055815:Young Adult
chemical_list: D016207:Cytokines