title: Identification of high-risk Dukes B colorectal cancer by microRNA expression profiling: a preliminary study.
abstract: OBJECTIVE
  MicroRNAs (miRNAs) from tumour tissue and common gene mutations were studied to determine whether they predict the development of metastasis in patients with Dukes B colorectal cancer.
  
  
  METHODS
  Patients who underwent curative resection for Dukes B colorectal cancer who subsequently developed distant metastatic disease at some stage in the following 5 years ('high-risk B') were compared with case-matched controls of Dukes A, Dukes B (no metastases, 'low-risk B') and Dukes C patients without any detectable metastasis at 5 years of follow-up. MiRNAs from tumour and adjacent normal tissue and common gene mutations (KRAS, BRAF, PIK3CA) in primary cancer tissue were analysed to identify prognostic tissue markers for the development of metastasis in patients with Dukes B colorectal cancer.
  
  
  RESULTS
  Expression of miR-15b and miR-135b was significantly downregulated (P < 0.001) in 'high-risk B' tumours compared with Dukes A, 'low-risk B' and C without metastasis. No significant differences were noted for mutation status and the development of metastasis.
  
  
  CONCLUSIONS
  The study suggests that the development of metastasis in Dukes B tumours may be predictable based on the miRNA expression of miR-15b and miR-135b. This requires further study on a much larger cohort.
authors.affiliation: Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.|Department of Colorectal Surgery, Leicester General Hospital, University Hospitals of Leicester NHS Trust, Leicester, UK.
authors.forename: M I
authors.initials: MI
authors.lastname: Aslam
authors.affiliation: Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.
authors.forename: J
authors.initials: J
authors.lastname: Venkatesh
authors.affiliation: Department of Colorectal Surgery, Leicester General Hospital, University Hospitals of Leicester NHS Trust, Leicester, UK.
authors.forename: J S
authors.initials: JS
authors.lastname: Jameson
authors.affiliation: Department of Histopathology, Leicester Royal Infirmary, University Hospitals of Leicester NHS Trust, Leicester, UK.
authors.forename: K
authors.initials: K
authors.lastname: West
authors.affiliation: Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.
authors.forename: J H
authors.initials: JH
authors.lastname: Pringle
authors.affiliation: Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, UK.|Department of Colorectal Surgery, Leicester General Hospital, University Hospitals of Leicester NHS Trust, Leicester, UK.
authors.forename: B
authors.initials: B
authors.lastname: Singh
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D058534:Class I Phosphatidylinositol 3-Kinases; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000235:genetics*; D009367:Neoplasm Staging / Q000379:methods; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D011379:Prognosis; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; C117307:KRAS protein, human; D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human; C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf; D016283:Proto-Oncogene Proteins p21(ras)
keywords: KRAS; MicroRNA profiling; colorectal cancer; metastatic disease; miR-135b; miR-15b