title: Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model.
abstract: OBJECTIVE
  To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysi after performing an experimental isolated lung contusion model.
  
  
  METHODS
  Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO) glutirthione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination.
  
  
  RESULTS
  The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after th pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C.
  
  
  CONCLUSIONS
  The present results indicate that vitamin C administration attenuated the oxidative damage and morphological change induced by pulmonary contusion in an experimental rat study.
authors.affiliation: Department  of Biochemistry, Dışkapı Yıldırım Beyazit Research Hospital, Ankara, Turkey.
authors.forename: Rana
authors.initials: R
authors.lastname: Sirmali
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Giniş
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sirmali
authors.forename: Okan
authors.initials: O
authors.lastname: Solak
authors.forename: Bengü
authors.initials: B
authors.lastname: Şeliman
authors.forename: Yetkin
authors.initials: Y
authors.lastname: Ağaçkiran
authors.forename: Namık
authors.initials: N
authors.lastname: Delibaş
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001205:Ascorbic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D018893:Bronchoalveolar Lavage; D002374:Catalase / Q000378:metabolism; D003288:Contusions; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D055370:Lung Injury; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D001205:Ascorbic Acid