title: Embryonal antitumor modulator of mkrtchyan prevents streptozotocin-induced damage of mouse and human pancreatic Beta cells in primary culture.
abstract: Restoration of beta cell functional activity is a cracial in diabetes type I treatment. Earlier, we have shown that embryonal antitumor modulator, created by prof. L. Mkrtchyan (EATM), expresses protective activity in rat diabetes model. In the present study, we sought to evaluate whether EATM directly acts on beta cell and protects them from streptozotocin (STZ) induced damage in primary mouse and human pancreatic beta cell cultures. Beta cells were isolated by treatment of whole pancreas (mouse) or piece of pancreatic tissue (human) with collagenase and trypsine. Human pancreatic beta cells were isolated from the piece of tissue obtained from caucasian 30-year old male undergoing surgery for benign pancreatic cysts. Beta cell damage was induced by STZ. Experiments carried out on mice beta cells showed higher effectiveness of EATM in concentration 0.01 mg/ml which almost completely abolished damage of cells induced by STZ in concentration 0.1 mg/ml and retained their functional activity. Experiments with human beta showed similar results and demonstrated EATM-induced stimulation of insulin production by intact beta cells.
authors.affiliation: M. Heratsi Yerevan State Medical University, Armenia.
authors.forename: Y
authors.initials: Y
authors.lastname: Aghajanova
authors.affiliation: M. Heratsi Yerevan State Medical University, Armenia.
authors.forename: H
authors.initials: H
authors.lastname: Harutyunyan
authors.affiliation: M. Heratsi Yerevan State Medical University, Armenia.
authors.forename: L
authors.initials: L
authors.lastname: Mkrtchyan
authors.affiliation: M. Heratsi Yerevan State Medical University, Armenia.
authors.forename: M
authors.initials: M
authors.lastname: Aghajanov
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy* / Q000503:physiopathology; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage* / Q000737:chemistry; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000473:pathology; D008297:Male; D051379:Mice; D061251:Primary Cell Culture; D011506:Proteins / Q000008:administration & dosage*; D051381:Rats
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D011506:Proteins