title: Preparative separation of echinocandin B from Aspergillus nidulans broth using macroporous resin adsorption chromatography.
abstract: Echinocandin B (ECB), an echinocandin type of lipopeptide antibiotic produced by Aspergillus nidulans, is a precursor for the synthesis of novel anti-fungal drug - anidulafungin. In this work, a separation strategy involving one-step macroporous resin adsorption chromatography was established for ECB purification from Aspergillus nidulans CCTCC M 2010275 fermentation broth. Among nine macroporous resin adsorbents tested, the non-polar resin HP-20 had the best adsorption and desorption performance. The static equilibrium adsorption data fitted well with the Langmuir equation, and the adsorption kinetic followed the pseudo-second order model. The separation parameters of ECB from broth were optimised by dynamic adsorption/desorption experiments with the column packed with HP-20 resin. Under optimal conditions, the purity increased by 3.8-fold from 23.2% in broth to 88.5% in eluent with 87.1% recovery yield by a one-step treatment. Our study provided a one-step and effective method for large-scale production of ECB, and offered references for separating other echinocandins from broth.
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Zou
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Qiu-Liang
authors.initials: QL
authors.lastname: Wang
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Kun
authors.initials: K
authors.lastname: Niu
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China. Electronic address: zhengyg@zjut.edu.cn.
authors.forename: Yu-Guo
authors.initials: YG
authors.lastname: Zheng
authors.affiliation: Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P.R China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, P.R China.
authors.forename: Yin-Chu
authors.initials: YC
authors.lastname: Shen
mesh_terms: D000327:Adsorption; D001233:Aspergillus nidulans / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D054714:Echinocandins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D054714:Echinocandins; D005656:Fungal Proteins; C007572:echinocandin B
keywords: Adsorption chromatography; Aspergillus nidulans; Broth; Echinocandin B; Macroporous resin