title: [Grippol, Vaxigrip and influvac vaccines--inductors of innate and adaptive immunity factor genes in human blood cells].
abstract: OBJECTIVE
  Study the effect of inactivated influenza vaccines on the activity of innate and adaptive immunity genes (TLR3, TLR4 and B2M), RNA-interference Dicer1-gene, production of cytokines (antiviral IFN type I and II, regulatory IL10, IL17) and pro-inflammatory factors IL1-β, TNFα.
  
  
  METHODS
  Gene expression was determined by rRT-PCR with authors' primers in human blood cells treated with various doses of the vaccines. Concentration of cytokines by enzyme immunoassay was measured in cultural fluid using "Vector-best" kits.
  
  
  RESULTS
  The studied vaccines have characteristic effects on genetic level. Grippol vaccine predominately stimulates TLR4 gene, activates TLR3, B2M and Dicer1 genes. Influvac vaccine mostly induces TLR3 gene and to a lesser extent TLR4 gene, does not influence the expression of B2M gene and inhibits Dicer1 gene. Vaxigrip split vaccine--the most potent stimulator of gene activity at low doses. Its main targets are TLR3 and B2M genes. All the inactivated vaccines--inductors of high level of IFNγ, low level of TNFα and do not induce IL17. Grippol additionally stimulates secretion of IL1-β, and Vaxigrip - IFNα. Subunit vaccines Grippol and Influvac that contain purified influenza virus hemagglutinins induce IL10 synthesis in blood cells.
  
  
  CONCLUSIONS
  Immunogenetic characteristics of the inactivated influenza vaccines administered nowadays are obtained.
authors.forename: T M
authors.initials: TM
authors.lastname: Sokolova
authors.forename: A N
authors.initials: AN
authors.lastname: Shuvalov
authors.forename: V V
authors.initials: VV
authors.lastname: Poloskov
authors.forename: I M
authors.initials: IM
authors.lastname: Shapoval
authors.forename: M P
authors.initials: MP
authors.lastname: Kostinov
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects / Q000235:genetics*; D001773:Blood Cells / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects / Q000235:genetics*; D009980:Influenza A virus / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000008:administration & dosage; D007251:Influenza, Human / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D007372:Interferons / Q000096:biosynthesis; D014612:Vaccines / Q000008:administration & dosage*; D015164:Vaccines, Inactivated / Q000008:administration & dosage
chemical_list: D016207:Cytokines; C408342:Grippol; D007252:Influenza Vaccines; D014612:Vaccines; D015164:Vaccines, Inactivated; C568705:influvac; C478242:vaxigrip; D007372:Interferons