title: Structural and biomechanical changes in the Achilles tendon after chronic treatment with statins.
abstract: Cases of tendinopathy and tendon ruptures have been reported as side effects associated with statin therapy. This work assessed possible changes in the structural and biomechanical properties of the tendons after chronic treatment with statins. Wistar rats were divided into the following groups: treated with atorvastatin (A-20 and A-80), simvastatin (S-20 and S-80) and the group that received no treatment (C). The doses of statins were calculated using allometric scaling, based on the doses of 80 mg/day and 20 mg/day recommended for humans. The morphological aspect of the tendons in A-20, S-20 and S-80 presented signals consistent with degeneration. Both the groups A-80 and S-80 showed a less pronounced metachromasia in the compression region of the tendons. Measurements of birefringence showed that A-20, A-80 and S-80 groups had a lower degree of organization of the collagen fibers. In all of the groups treated with statins, the thickness of the epitenon was thinner when compared to the C group. In the biomechanical tests the tendons of the groups A-20, A-80 and S-20 were less resistant to rupture. Therefore, statins affected the organization of the collagen fibers and decreased the biomechanical strength of the tendons, making them more predisposed to ruptures.
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil. Electronic address: leprado.farm@gmail.com.
authors.forename: L P
authors.initials: LP
authors.lastname: de Oliveira
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: C P
authors.initials: CP
authors.lastname: Vieira
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: F D
authors.initials: FD
authors.lastname: Guerra
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Almeida
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, University of Campinas - UNICAMP, Campinas, SP, Brazil.
authors.forename: E R
authors.initials: ER
authors.lastname: Pimentel
mesh_terms: D000125:Achilles Tendon / Q000033:anatomy & histology / Q000187:drug effects* / Q000293:injuries*; D000818:Animals; D000069059:Atorvastatin / Q000494:pharmacology; D003094:Collagen / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012421:Rupture; D019821:Simvastatin / Q000494:pharmacology; D013708:Tendon Injuries / Q000473:pathology*
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D003094:Collagen; D000069059:Atorvastatin; D019821:Simvastatin
keywords: Biomechanics; Birefringence; Collagen; Ruptures; Statins; Tendinopathy