title: A comparative study on the therapeutic effects of silymarin and silymarin-loaded solid lipid nanoparticles on D-GaIN/TNF-α-induced liver damage in Balb/c mice.
abstract: Nanostructure-mediated drug delivery is known to have a potential to improve drug bioavailability, apart from fostering release deviation of drug molecules and enabling precision drug targeting. Solid lipid nanoparticles (SLNs) have drawn great deal of the attention of scientists in finding a solution to minimize pharmaceutic limitations of the drugs used. Silymarin (Sm) has so far been used for treating diverse liver and gallbladder disorders, such as cirrhosis, hepatitis, and jaundice, and for protecting the liver against poisoning from chemical and environmental toxins on account of its antihepatotoxic and antioxidative properties. The present study aims to develop a novel silymarin-loaded solid lipid nanoparticle (Sm-loaded SLN) system with enhanced bioavailability and with an ability to provide excellent hepatic protection for poorly water-soluble drugs. Based upon our investigation results with apoptotic markers, PCNA and lightmicroscopic findings, it can be concluded that Sm-loaded SLN significantly reduced D-GaIN/TNF-α-induced hepatotoxicity, which suggested improved bioactivity compared to Sm. In conclusion, Sm-loaded SLN could be a useful system for the delivery of poorly water-soluble Sm, apart from providing favourable hepatic protection.
authors.affiliation: Faculty of Science, Department of Biology, Anadolu University, Eskisehir, Turkey. Electronic address: mustafacengizogu@gmail.com.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Cengiz
authors.affiliation: Faculty of Science, Department of Biology, Anadolu University, Eskisehir, Turkey.
authors.forename: H Mehtap
authors.initials: HM
authors.lastname: Kutlu
authors.affiliation: Faculty of Medicine, Department of Histology and Embryology, Osmangazi University, Eskisehir, Turkey.
authors.forename: Dilek D
authors.initials: DD
authors.lastname: Burukoglu
authors.affiliation: Faculty of Arts and Science, Department of Biology, Osmangazi University, Eskisehir, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Ayhancı
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016503:Drug Delivery Systems; D005688:Galactosamine / Q000009:adverse effects*; D008055:Lipids / Q000737:chemistry*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D020011:Protective Agents / Q000494:pharmacology; D012838:Silymarin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D008055:Lipids; D020011:Protective Agents; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha; D005688:Galactosamine
keywords: Balb/c mice; D-GaIN/TNF-α; Hepatoprotectivity; Hepatotoxicity; Silymarin-loaded solid lipid nanoparticle