title: Increased ARPP-19 expression is associated with hepatocellular carcinoma.
abstract: The cAMP-regulated phosphoprotein 19 (ARPP-19) plays a key role in cell mitotic G2/M transition. Expression of ARPP-19 was increased in human hepatocellular carcinoma (HCC) compared to adjacent non-tumorous liver tissues in 36 paired liver samples, and the level of ARPP-19 in HCC tissues was positively correlated with the tumor size. To determine the interrelationship between ARPP-19 expression and HCC, we silenced ARPP-19 expression in the human hepatocarcinoma HepG2 and SMMC-7721 cells using lentivirus encoding ARPP-19 siRNA. HepG2 and SMMC-7721 cells with ARPP-19 knockdown displayed lowered cell growth rate, retarded colony formation and increased arrest at the G2/M phase transition. Silencing ARPP-19 in HCC cells resulted in decreased protein levels of phospho-(Ser) CDKs substrates and increased levels of inactivated cyclin division cycle 2 (Cdc2). Therefore, ARPP-19 may play a role in HCC pathogenesis through regulating cell proliferation.
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. songhy@126.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Song
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. yearlca@126.com.
authors.forename: Jielu
authors.initials: J
authors.lastname: Pan
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. liuyang8753com@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. ellawen928@yahoo.com.
authors.forename: Hongzhu
authors.initials: H
authors.lastname: Wen
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. et1978@126.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China. jiefengcui@hotmail.com.
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Cui
authors.affiliation: Liver Cancer Institute of Zhongshan Hospital, Fudan University, Shanghai 200032, China. ykliu@zshospital.com.
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Oncology and Institute of Traditional Chinese Medicine in Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. beearhu@hotmail.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Hu
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, Canada. zyao@uottawa.ca.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. jiliver@vip.sina.com.
authors.forename: Guang
authors.initials: G
authors.lastname: Ji
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016203:CDC2 Protein Kinase; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D016022:Case-Control Studies; D049109:Cell Proliferation; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D010750:Phosphoproteins; C064321:cyclic AMP-regulated phosphoprotein 19; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases