title: Prominent IL-12 production and tumor reduction in athymic nude mice after Toxoplasma gondii lysate antigen treatment.
abstract: Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: Kyoung-Ho
authors.initials: KH
authors.lastname: Pyo
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: Bong-Kwang
authors.initials: BK
authors.lastname: Jung
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Xin
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: You-Won
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea.
authors.forename: Jong-Yil
authors.initials: JY
authors.lastname: Chai
authors.affiliation: Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. ; Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Shin
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004195:Disease Models, Animal; D007113:Immunity, Innate; D007167:Immunotherapy / Q000379:methods*; D018664:Interleukin-12 / Q000097:blood*; D008264:Macrophages / Q000276:immunology; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053594:Myeloid Differentiation Factor 88 / Q000032:analysis; D009369:Neoplasms / Q000473:pathology / Q000628:therapy*; D014122:Toxoplasma / Q000276:immunology*; D016896:Treatment Outcome
chemical_list: D000953:Antigens, Protozoan; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D018664:Interleukin-12
keywords: IL-12; MyD88; NF-κB; TIMP-1; Toxoplasma gondii; anti-tumorigenesis; athymic mouse