title: Analysis of the transcriptomic profilings of Mandarin fish (Siniperca chuatsi) infected with Flavobacterium columnare with an emphasis on immune responses.
abstract: Flavobacterium columnare (FC) is the causative pathogen of columnaris which has caused great economic loss in fish culture worldwide, including in Mandarin fish (Siniperca chuatsi) culture. In the present study, the transcriptomic profiles of the head kidneys from FC-infected and non-infected Mandarin fish were obtained using HiSeq™ 2000 (Illumina). Totally 31,168 unigenes with high quality were obtained. Genes involved in protein folding, metabolism and energy, immune responses, oxidoreductase activity, cell growth and death were identified as enriched classes. 1019 differently expressed genes between the two groups were identified, including 603 up-regulated and 416 down-regulated genes. 27 differently expressed immune related genes were scrutinized, including 17 up-regulated and 10 down-regulated genes. Six of the differently expressed genes were further validated by qRT-PCR. The roles of the immune related genes were discussed. Identification of the host genes in response to FC infection will shed a new light on the prevention of columnaris.
authors.affiliation: Wuhan Fishery Research Institute, 430207 Wuhan, Hubei, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Yulei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Division of Science and Technology, Beijing Normal University - Hong Kong Baptist University United International College, 519085 Zhuhai, Guangdong, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Yachan
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Asim
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China. Electronic address: xfliang@mail.hzau.edu.cn.
authors.forename: Xufang
authors.initials: X
authors.lastname: Liang
authors.affiliation: Wuhan Fishery Research Institute, 430207 Wuhan, Hubei, China.
authors.forename: Taoshan
authors.initials: T
authors.lastname: Ai
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, 430070 Wuhan, Hubei, China; China Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, 430070 Wuhan, Hubei, China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D045826:Flavobacteriaceae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000502:physiology*; D007113:Immunity, Innate; D008969:Molecular Sequence Data; D010473:Perciformes; D017422:Sequence Analysis, DNA / Q000662:veterinary; D059467:Transcriptome
chemical_list: D029941:Fish Proteins
keywords: Differently expressed genes; Flavobacterium columnare; Immune responses; Siniperca chuatsi; Transcriptome