title: Dietary blueberry and bifidobacteria attenuate nonalcoholic fatty liver disease in rats by affecting SIRT1-mediated signaling pathway.
abstract: NAFLD model rats were established and divided into NAFLD model (MG group), SIRT1 RNAi (SI group), blueberry juice (BJ group), blueberry juice + bifidobacteria (BJB group), blueberry juice + SIRT1 RNAi (BJSI group), and blueberry juice + bifidobacteria + SIRT1 RNAi groups (BJBSI group). A group with normal rats was a control group (CG). BJB group ameliorated NAFLD, which was better than BJ group (P < 0.05). The lipid accumulation was lower in CG, BJ, and BJB groups than that in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SIRT1 and PPAR-α were higher in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The levels of SREBP-1c were lower in CG, BJ, and BJB groups than those in MG, SI, BJSI, and BJBSI groups (P < 0.05). The biochemical indexes SOD, GSH, and HDL-c were improved from CG to BJB group (P < 0.05). Inversely, the levels of AST and ALT, TG, TC, LDL-c, and MDA were decreased from CG to BJB group (P < 0.05). These changes enhance antioxidative capability and biochemical index of rats. Blueberry juice and bifidobacteria improve NAFLD by activating SIRTI-mediating signaling pathway.
authors.affiliation: Biochemistry Department, Affiliated Hospital of Guiyang Medical College, Guiyang, Guizhou 550004, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Ren
authors.affiliation: Guiyang Medical College, Guiyang, Guizhou 550004, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Huang
authors.affiliation: Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, 28 Guiyi Street, Guiyang, Guizhou 550004, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001628:Beverages; D001644:Bifidobacterium; D029798:Blueberry Plants; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005638:Fruit; D008297:Male; D065626:Non-alcoholic Fatty Liver Disease / Q000178:diet therapy / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D018384:Oxidative Stress / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012307:Risk Factors; D015398:Signal Transduction; D056564:Sirtuin 1 / Q000378:metabolism*
chemical_list: C488368:Sirt1 protein, rat; D056564:Sirtuin 1