title: Polyhydroxyalkanoates from Pseudomonas sp. using synthetic and olive mill wastewater under limiting conditions.
abstract: The present study aimed at investigating the ability of bacteria isolated from an enriched mixed culture to produce polyhydroxyalkanoates (PHAs) and examining the effect of nitrogen and dual nitrogen-oxygen limitation on PHAs production, by using both synthetic and olive mill wastewater (OMW). PHAs production was performed through batch experiments using both the enriched culture and the isolated strains (belonging to the genus of Pseudomonas) aiming to compare PHAs accumulation capacity, yields and rates. The use of enriched culture and synthetic wastewater under nitrogen limitation resulted in the highest PHA accumulation, i.e. 64.4%gPHAs/g of cell dry mass (CDM). However, when OMW was used, PHAs accumulation significantly decreased, i.e. 8.8%gPHAs/g CDM. The same trend was followed by the isolated strains, nevertheless, their ability to synthesize PHAs was lower. Although, dual nitrogen-oxygen limitation generally slowed down PHAs biosynthesis, in certain strains PHAs production was positively affected.
authors.affiliation: Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 1 Caratheodory Str., 26500 Patras, Greece; Institute of Chemical Engineering Sciences, Foundation for Research & Technology Hellas (ICE-HT/FORTH), 26504 Patras, Greece. Electronic address: ckourmentza@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Kourmentza
authors.affiliation: Institute of Chemical Engineering Sciences, Foundation for Research & Technology Hellas (ICE-HT/FORTH), 26504 Patras, Greece.
authors.forename: I
authors.initials: I
authors.lastname: Ntaikou
authors.affiliation: Institute of Chemical Engineering Sciences, Foundation for Research & Technology Hellas (ICE-HT/FORTH), 26504 Patras, Greece; School of Chemical Engineering, National Technical University of Athens (NTUA), Zografou Campus, 15780 Athens, Greece.
authors.forename: G
authors.initials: G
authors.lastname: Lyberatos
authors.affiliation: Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, 1 Caratheodory Str., 26500 Patras, Greece.
authors.forename: M
authors.initials: M
authors.lastname: Kornaros
mesh_terms: D001711:Biotransformation; D007220:Industrial Waste; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis* / Q000737:chemistry*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D062065:Wastewater
chemical_list: D007220:Industrial Waste; D009584:Nitrogen; D010100:Oxygen; D054813:Polyhydroxyalkanoates; D062065:Wastewater
keywords: Nitrogen oxygen limitation; Olive mill wastewater; Polyhydroxyalkanoates (PHAs); Pseudomonas