title: Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction.
abstract: Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan ; Department of Immunobiology and Pathogenic Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Laboratory of Animal Breeding and Genetics, Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Fukuda
authors.affiliation: Laboratory of Applied Physics, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Bai
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kuroda
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Rintaro
authors.initials: R
authors.lastname: Tomioka
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoneyama
authors.affiliation: Laboratories of Animal Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
keywords: Antibody Against Leptospiral Lipopolysaccharide; Cytokines; Leptospiral Lipopolysaccharide; Pig Fibroblast Cell Line; Toll-like Receptor 2