title: Regulatory motifs on ISWI chromatin remodelers: molecular mechanisms and kinetic proofreading.
abstract: Recently, kinetic proofreading scenarios have been proposed for the regulation of chromatin remodeling, first on purely theoretical grounds (Blossey and Schiessel 2008 HFSP J. 2 167-70) and deduced from experiments on the ISWI/ACF system (Narlikar 2010 Curr. Opin. Chem. Biol. 14 660). In the kinetic proofreading scenario of chromatin remodeling, the combination of the recognition of a histone tail state and ATP-hydrolysis in the remodeler motor act together to select (i.e. proofread) a nucleosomal substrate. ISWI remodelers have recently been shown to have an additional level of regulation as they contain auto-inhibitory motifs which need to be inactivated through an interaction with the nucleosome. In this paper we show that the auto-regulatory effect enhances substrate recognition in kinetic proofreading. We further report some suggestive additional insights into the molecular mechanism underlying ISWI-autoregulation.
authors.affiliation: Interdisciplinary Research Institute, Université des Sciences et des Technologies de Lille (USTL), CNRS USR3078, 50 Avenue Halley, 59568 Villeneuve d'Ascq, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Brysbaert
authors.forename: Marc F
authors.initials: MF
authors.lastname: Lensink
authors.forename: Ralf
authors.initials: R
authors.lastname: Blossey
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D020816:Amino Acid Motifs; D042002:Chromatin Assembly and Disassembly; D006657:Histones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006706:Homeostasis; D007700:Kinetics; D008958:Models, Molecular; D009690:Nucleic Acid Conformation
chemical_list: D006657:Histones; D000255:Adenosine Triphosphate