title: Effect of Cyclophosphamide on Expression of MMP-9 and TGF-β1 in Renal Tissue of Rats with Diabetes Mellitus.
abstract: Diabetic nephropathy (DN) is a severe microvascular complication that occurs in diabetes patients. In this study, we investigated the effect of cyclophosphamide (CTX) on expression of matrix metalloproteinases (MMP-9) and transforming growth factor-β1 (TGF-β1) in renal tissue of rats with DN. We found CTX significantly reduces KI and blood glucose levels, microalbuminuria, and blood urea nitrogen. It also decreases the expression of TGF-β1 and increases the expression of MMP-9. Our data suggest CTX may reduce the excretion of urine proteins, alleviate kidney hypertrophy, and reduce blood glucose level through regulation of TGF-β1 and MMP-9 gene expression.
authors.affiliation: Department of Nephrology, 5th Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Nephrology, 1st Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Nephrology, 5th Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Nephrology, 5th Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China. drzhangjin01@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003520:Cyclophosphamide / Q000494:pharmacology*; D003928:Diabetic Nephropathies / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D009584:Nitrogen / Q000097:blood / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D053773:Transforming Growth Factor beta1; D003520:Cyclophosphamide; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat; D009584:Nitrogen
keywords: Cyclophosphamide; Diabetic nephropathy; Matrix metalloproteinase; Transforming growth factor-β1