title: Creation of DNA aptamers against recombinant bone morphogenetic protein 15.
abstract: The oocyte-derived growth factor bone morphogenetic protein (BMP) 15 plays important roles in fertility, but its mechanism of action differs between species. Generation of BMP15-binding molecules, as an essential investigation tool, would be helpful to provide valuable insight into the underlying biological features of BMP15. The BMP15-binding molecules could be antibodies or aptamers. Aptamers have many advantages over antibodies as macromolecular ligands for target proteins. DNA aptamers can be obtained by a method of Systematic Evolution of Ligands by EXponential enrichment (SELEX) beginning with a pool of random sequences. However, the success of this technique cannot be guaranteed if the initial pool lacks candidate sequences. Herein, we report on the creation of DNA aptamers by means of modified SELEX. The modification included enhanced mutation and progressive selection during an in vitro evolutionary process. As a proof-of-principle, we started from a single sequence instead of a multiple-sequence pool. Functional aptamers against the recombinant BMP15 were successfully created and identified.
authors.affiliation: Molecular Medicine Research Institute, Huaqiao University, 269 Chenghua North Rd, Quanzhou, Fujian 362021, China.|Corresponding author. Email: junshenglin@hqu.edu.cn.
authors.forename: Jun Sheng
authors.initials: JS
authors.lastname: Lin
authors.affiliation: Animal Reproduction, AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New Zealand.
authors.forename: Alexia
authors.initials: A
authors.lastname: Kauff
authors.affiliation: Molecular Medicine Research Institute, Huaqiao University, 269 Chenghua North Rd, Quanzhou, Fujian 362021, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Diao
authors.affiliation: Molecular Medicine Research Institute, Huaqiao University, 269 Chenghua North Rd, Quanzhou, Fujian 362021, China.
authors.forename: Huiyong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Animal Reproduction, AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New Zealand.
authors.forename: Steve
authors.initials: S
authors.lastname: Lawrence
authors.affiliation: Animal Reproduction, AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel 9053, New Zealand.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Juengel
keywords: Systematic Evolution of Ligands by EXponential enrichment (SELEX); antibody; evolution; mutation