title: On-chip multi-gas incubation for microfluidic cell cultures under hypoxia.
abstract: We developed a simple system that regulates CO2 and O2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO2 and desired pO2 levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth.
authors.affiliation: Department of Materials and Life Sciences, Tokyo Denki University , Saitama 350-0394, Japan.
authors.forename: Atsushi
authors.identifier: 0000-0002-8664-4821
authors.initials: A
authors.lastname: Takano
authors.affiliation: Department of Materials and Life Sciences, Tokyo Denki University , Saitama 350-0394, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Department of Mechanical Engineering, Shibaura Institute of Technology , Tokyo 135-8548, Japan.
authors.forename: Nobuyuki
authors.identifier: 0000-0001-9341-7304
authors.initials: N
authors.lastname: Futai