title: Biotechnological potential of yeast isolates from cachaça: the Brazilian spirit.
abstract: This study identified phenotypic traits appropriate for biotechnological applications of 118 yeasts isolated from cachaça distilleries. Different properties were verified: capacity to use alternative carbon sources; ability to tolerate high concentrations of sucrose, ethanol, methanol, aluminum and zinc as well as different pH values and foam production. Pichia guilliermondii and Pichia anomala strains were identified as the most promising ones for application in the second-generation biofuel industry, showing ability to grow on high glycerol concentrations. Other isolates, identified as Saccharomyces cerevisiae, produced bioethanol comparable to the industrial strains, and were therefore ideal for use in the first-generation ethanol industry. Some of these strains also showed high resistance to aluminum, as observed in sugarcane juice, and to inter-cycle washings with diluted sulphuric acid, as performed in the industrial bioethanol production process. In summary, yeast isolates from cachaça distilleries displayed robustness and phenotypic plasticity, which makes them interesting for biotechnological applications.
authors.affiliation: Lab. de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Universidade Federal de Ouro Preto, Campus Do Morro Do Cruzeiro, Ouro Preto, MG, Brazil.
authors.forename: Luís Eduardo Fernandes Rodrigues
authors.initials: LE
authors.lastname: da Conceição
authors.forename: Margarete Alice Fontes
authors.initials: MA
authors.lastname: Saraiva
authors.forename: Raphael Hermano Santos
authors.initials: RH
authors.lastname: Diniz
authors.forename: Juliana
authors.initials: J
authors.lastname: Oliveira
authors.forename: Gustavo Dimas
authors.initials: GD
authors.lastname: Barbosa
authors.forename: Florencia
authors.initials: F
authors.lastname: Alvarez
authors.forename: Lygia Fátima da Mata
authors.initials: LF
authors.lastname: Correa
authors.forename: Hygor
authors.initials: H
authors.lastname: Mezadri
authors.forename: Mauricio Xavier
authors.initials: MX
authors.lastname: Coutrim
authors.forename: Robson José de Cássia Franco
authors.initials: RJ
authors.lastname: Afonso
authors.forename: Candida
authors.initials: C
authors.lastname: Lucas
authors.forename: Ieso Miranda
authors.initials: IM
authors.lastname: Castro
authors.forename: Rogelio Lopes
authors.initials: RL
authors.lastname: Brandão
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology; D000535:Aluminum / Q000032:analysis; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D001938:Brazil; D057168:Distillation; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000032:analysis; D010843:Pichia / Q000145:classification / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification*; D013395:Sucrose / Q000032:analysis; D015032:Zinc / Q000032:analysis
chemical_list: D056804:Biofuels; D000431:Ethanol; D013395:Sucrose; D000535:Aluminum; D015032:Zinc; D005990:Glycerol; D000432:Methanol