title: Characterisation of oligosaccharides from Baizhu by HILIC-MS.
abstract: Polysaccharides from Baizhu were separated by preparative hydrophilic interaction liquid chromatography on an XAmide column and its components were characterised as inulin-type polysaccharides with structures of α-D-glucopyranosyl-[-(1 → 2) -β-D-fructofuranosyl-](n -1)-(1 → 2) -β-D-fructofuranoside (n = 3-20) by a combinatory application of electrospray-ionisation mass spectrometry, nuclear magnetic resonance and IR, as well as the chemical analysis of monosaccharide composition. In addition, the contents of nystose and 1F-fructofranosylnystose in the crude and purified Baizhu polysaccharides were determined to be 5.81%, 4.92% and 0.70%, 0.84% (w/w), respectively. In addition, MTT assay indicated that the Baizhu polysaccharides could effectively promote spleen lymphocyte transformation for the enhancement of organism immunity. It is for the first time that inulin-type polysaccharides were discovered in Baizhu and its immuno-enhancing activity was reported, which is a vigorous evidence to explain the efficacy of Baizhu.
authors.affiliation: a College of Pharmacy, Liaoning University of Traditional Chinese Medicine , Dalian 116600 , P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Lin
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Qu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Shi
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Dou
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
mesh_terms: D000818:Animals; D031174:Atractylodes / Q000737:chemistry*; D002853:Chromatography, Liquid; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D027343:Rhizome / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D013154:Spleen / Q000166:cytology
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; C043600:fungitetraose
keywords: Atractylodes macrocephala Koidz; XAmide column; inulin-type oligosaccharides; spleen lymphocyte transformation