title: Disruption of Snf3/Rgt2 glucose sensors decreases lifespan and caloric restriction effectiveness through Mth1/Std1 by adjusting mitochondrial efficiency in yeast.
abstract: Down-regulation of intracellular nutrient signal pathways was proposed to be a primary mechanism of caloric restriction (CR)-mediated lifespan extension. However, the link between lifespan and glucose sensors in the plasma membrane was poorly understood in yeast. Herein, a mutant that lacked glucose sensors (snf3Δrgt2Δ) had impaired glucose fermentation, showed decreased chronological lifespan (CLS), and reduced CLS extension by CR. The mutant also had reduced mitochondrial efficiency, as inferred by increased mitochondrial superoxide and decreased ATP levels. Mth1 and Std1, which are downstream effectors of the Snf3/Rgt2 pathway, were required for viability through mitochondrial function but not fermentative metabolism.
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Kyung-Mi
authors.initials: KM
authors.lastname: Choi
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
authors.forename: Young-Yon
authors.initials: YY
authors.lastname: Kwon
authors.affiliation: Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea. Electronic address: cklee2005@korea.ac.kr.
authors.forename: Cheol-Koo
authors.initials: CK
authors.lastname: Lee
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D031204:Caloric Restriction; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D008136:Longevity / Q000235:genetics* / Q000502:physiology; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics*; D009154:Mutation; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D047908:Intracellular Signaling Peptides and Proteins; C118806:MTH1 protein, S cerevisiae; D009004:Monosaccharide Transport Proteins; C112512:RGT2 protein, S cerevisiae; C104463:SNF3 protein, S cerevisiae; C081335:STD1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D005947:Glucose
keywords: Chronological lifespan; Glucose sensor; Gpr1; Mitochondrial function; Rgt2; Snf3