title: Divergent signalling pathways regulate lipopolysaccharide-induced eRNA expression in human monocytic THP1 cells.
abstract: Recent studies have indicated that non-coding RNAs transcribed from enhancer regions are important regulators of enhancer function and gene expression. In this report, we have characterised the expression of six enhancer RNAs (eRNAs) induced in human monocytic THP1 cells following activation of the innate immune response by lipopolysaccharide (LPS). Specifically, we have demonstrated that LPS-induced expression of individual eRNAs is mediated through divergent intracellular signalling pathways that includes NF-κB and the mitogen activated protein kinases, extracellular regulated kinase-1/2 and p38.
authors.affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, United Kingdom.
authors.forename: James A
authors.initials: JA
authors.lastname: Heward
authors.affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, United Kingdom.
authors.forename: Benoit T
authors.initials: BT
authors.lastname: Roux
authors.affiliation: Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, United Kingdom. Electronic address: m.a.lindsay@bath.ac.uk.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Lindsay
mesh_terms: D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D062085:RNA, Long Noncoding; D048051:p38 Mitogen-Activated Protein Kinases
keywords: ERK and p38; Enhancer RNA; Lipopolysaccharide; Long non-coding RNA; Monocyte; NF-κB