title: A sensitive fluorescent assay for thiamine based on metal-organic frameworks with intrinsic peroxidase-like activity.
abstract: Metal-organic frameworks (MOFs) with tunable structures and properties have recently been emerged as very interesting functional materials. However, the catalytic properties of MOFs as enzymatic mimics remain to be further investigated. In this work, we for the first time demonstrated the peroxidase-like activity of copper-based MOFs (HKUST-1) by employing thiamine (TH) as a peroxidase substrate. In the presence of H2O2, HKUST-1 can catalyze efficiently the conversion of non-fluorescent TH to strong fluorescent thiochrome. The catalytic activity of HKUST-1 is highly dependent on the temperature, pH and H2O2 concentrations. As a peroxidase mimic, HKUST-1 not only has the features of low cost, high stability and easy preparation, but also follows Michaelis-Menten behaviors and shows stronger affinity to TH than horseradish peroxidase (HRP). Based on the peroxidase-like activity of HKUST-1, a simple and sensitive fluorescent method for TH detection has been developed. As low as 1 μM TH can be detected with a linear range from 4 to 700 μM. The detection limit for TH is about 50 fold lower than that of HRP-based fluorescent assay. The proposed method was successfully applied to detect TH in tablets and urine samples and showed a satisfactory result. We believed that the present work could improve the understanding of catalytic behaviors of MOFs as enzymatic mimics and find out a wider application in bioanalysis.
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China. Electronic address: hltan@jxnu.edu.cn.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Tan
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Zhengchen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Chanjiao
authors.initials: C
authors.lastname: Ma
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Song
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China.
authors.forename: Fugang
authors.initials: F
authors.lastname: Xu
authors.affiliation: Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Key Laboratory of Chemical Biology, Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, PR China. Electronic address: lwanggroup@aliyun.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D006801:Humans; D009942:Organometallic Compounds / Q000737:chemistry*; D009195:Peroxidase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013607:Tablets / Q000737:chemistry; D013831:Thiamine / Q000032:analysis* / Q000737:chemistry / Q000652:urine
chemical_list: D002264:Carboxylic Acids; D009942:Organometallic Compounds; D013607:Tablets; D003300:Copper; D009195:Peroxidase; D013831:Thiamine
keywords: Fluorescence; Metal-organic frameworks; Peroxidase-like activity; Thiamine