title: Regulation of gene expression in HBV- and HCV-related hepatocellular carcinoma: integrated GWRS and GWGS analyses.
abstract: OBJECTIVE
  To explore the molecular mechanism of hepatitis B virus-related and hepatitis C virus-related hepatocellular carcinoma, samples from hepatitis B virus and hepatitis C virus infected patients and the normal were compared, respectively.
  
  
  METHODS
  In both experiments, genes with high value were selected based on a genome-wide relative significance and genome-wide global significance model. Co-expression network of the selected genes was constructed, and transcription factors in the network were identified. Molecular complex detection algorithm was used to obtain sub-networks.
  
  
  RESULTS
  Based on the new model, the top 300 genes were selected. Co-expression network was constructed and transcription factors were identified. We obtained two common genes FCN2 and CXCL14, and two common transcription factors RFX5 and EZH2. In hepatitis B virus experiment, cluster 1 and 3 had the higher value. In cluster 1, ten of the 17 genes and one transcription factor were all reported associated with hepatocellular carcinoma. In cluster 3, transcription factor ESR1 was reported related with hepatocellular carcinoma. In hepatitis C virus experiment, the value of cluster 3 and 4 was higher. In cluster 3, nine genes were reported to play a key role in hepatocellular carcinoma. In cluster 4, there were 5 genes in the 34 genes. To compare the relevance of a node in holding together communicating nodes, centralities based analysis was performed and we obtained some genes with high stress value.
  
  
  CONCLUSIONS
  The analysis above helped us to understand the pathogenesis of hepatitis B virus and hepatitis C virus associated hepatocellular carcinoma.
authors.affiliation: Department of General Surgery, Provincial Hospital Affiliated to Shandong University Ji'nan 250014, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Provincial Hospital Affiliated to Shandong University Ji'nan 250014, China.
authors.forename: Hua-Qiang
authors.initials: HQ
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Provincial Hospital Affiliated to Shandong University Ji'nan 250014, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
keywords: Hepatocellular carcinoma; hepatitis B virus; hepatitis C virus; transcription factor