title: Two-liquid phase partitioning biotrickling filters for methane abatement: exploring the potential of hydrophobic methanotrophs.
abstract: The potential of two-liquid phase biotrickling filters (BTFs) to overcome mass transfer limitations derived from the poor aqueous solubility of CH4 has been scarcely investigated to date. In this context, the abatement of diluted methane emissions in two-liquid phase BTFs was evaluated using two different inocula: a type II methanotrophs culture in BTF 1 and a hydrophobic microbial consortium capable of growing inside silicone oil in BTF 2. Both BTFs supported stable elimination capacities above 45 g m(-3) h(-1) regardless of the inoculum, whereas no improvement derived from the presence of hydrophobic microorganisms compared to the type II metanotrophs culture was observed. Interestingly, the addition of silicone oil mediated a reduced metabolites concentration in the recycling aqueous phase, thus decreasing the needs for mineral medium renewal. Moreover, a 78% similarity was recorded between the microbial communities enriched in both BTFs at the end of the experimental period in spite of the differences in the initial inoculum structure. The results obtained confirmed the superior performance of two-liquid phase BTFs for CH4 abatement compared with conventional biotrickling filters.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Lebrero
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Hernández
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Pérez
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Estrada
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Spain. Electronic address: mutora@iq.uva.es.
authors.forename: Raúl
authors.initials: R
authors.lastname: Muñoz
mesh_terms: D000393:Air Pollutants / Q000737:chemistry; D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D019149:Bioreactors; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D008697:Methane / Q000737:chemistry*; D014867:Water; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D000393:Air Pollutants; D014874:Water Pollutants, Chemical; D014867:Water; D008697:Methane
keywords: Biotrickling filter; Greenhouse gas; Mass transfer; Methane; Silicone oil; Two-liquid phase partitioning bioreactor