title: Syndecan-1 modulates the motility and resolution responses of macrophages.
abstract: OBJECTIVE
  Syndecan-1 (Sdc-1) is a member of a family of cell surface proteoglycans, which has been reported to participate in the regulation of events relevant to tissue repair and chronic injury responses, including cell-substrate interactions, matrix remodeling, and cell migration. In this study, we report the functional significance of Sdc-1 in polarized macrophage populations and its role in adhesion and motility events relevant to resolution of the inflammatory program.
  
  
  RESULTS
  Macrophage Sdc-1 expression is associated with differentiated M2 macrophages with high intrinsic motility, and Sdc-1 deficiency is characterized by impaired migration and enhanced adhesion. Leukocyte infiltration and emigration were examined in a thioglycollate-induced model of peritonitis in Sdc-1(+/+) and Sdc-1(-/-) mice. Although the infiltration of inflammatory cells was similar in both cohorts, a significant delay in the lymphatic clearance of Sdc-1(-/-) macrophages was observed. Moreover, we observed enhanced inflammation and greater burden of atherosclerotic plaques in ApoE(-/-)Sdc-1(-/-) mice maintained on a Western diet.
  
  
  CONCLUSIONS
  These results demonstrate that defective motility in Sdc-1(-/-) macrophages promotes a persistent inflammatory state with relevance to the pathogenesis of atherosclerosis.
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Julianty
authors.initials: J
authors.lastname: Angsana
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Jiaxuan
authors.initials: J
authors.lastname: Chen
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Sumona
authors.initials: S
authors.lastname: Smith
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Jiantao
authors.initials: J
authors.lastname: Xiao
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.).
authors.forename: Liying
authors.initials: L
authors.lastname: Liu
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.). echaikof@bidmc.harvard.edu challer@bidmc.harvard.edu.
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Haller
authors.affiliation: From the Department of Bioengineering, Georgia Institute of Technology, Atlanta (J.A.); Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA (J.C., L.L., C.A.H., E.L.C.); Department of Surgery, Emory University, Atlanta, GA (S.S., J.X., J.W.); and Wyss Institute of Biologically Inspired Engineering, Harvard University, Boston, MA (E.L.C.). echaikof@bidmc.harvard.edu challer@bidmc.harvard.edu.
authors.forename: Elliot L
authors.initials: EL
authors.lastname: Chaikof
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002633:Chemotaxis; D002634:Chemotaxis, Leukocyte; D017077:Culture Media, Conditioned; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D006801:Humans; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010641:Phenotype; D058226:Plaque, Atherosclerotic; D015398:Signal Transduction; D053668:Syndecan-1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D001057:Apolipoproteins E; D017077:Culture Media, Conditioned; C508242:Sdc1 protein, mouse; D053668:Syndecan-1
keywords: cell movement; macrophage; syndecan-1