title: Effects of different crosslinking methods on the properties of collagen-calcium phosphate composite materials.
abstract: The purpose of this study is the preparation and characterization of porous collagen/calcium phosphates (Col/CaP) composites. Collagen scaffolds with high porosity were prepared by freeze-drying technique. Col/CaP scaffold were created by new method--by deposition of calcium phosphate within collagen matrix in two steps using freeze-drying process before immersing samples in calcium solution. To find the optimal preparative method, we prepared diverse Col/CaP scaffolds using different collagen concentration and various crosslinking method: crosslinking with carbodiimide (EDC/NHS) and dehydrothermal treatment (DHT). This study explores the effect of the different crosslinking method on the properties of scaffolds, such as: microstructure (porosity and density), dissolution, water uptake, mechanical properties and collagenase degradation. The results obtained showed that crosslinking the scaffolds by either EDC/NHS or DHT have good mechanical and morphological properties compatible with their potential application in bone regeneration. The results demonstrated that properties of Col/CaP scaffolds changed significantly with different crosslinking method. However, while EDC/NHS increased the scaffolds' resistance to dissolution and degradation by collagenase, DHT decreased the swelling ratio and resistance to dissolution in PBS solution. Based on our study, 2% collagen concentration and EDC/NHS as crosslinking reagent are recommended to design the scaffold for use in bone engineering.
authors.affiliation: Faculty of Chemistry, N. Copernicus University, Gagarin 7, 87-100 Torun, Poland. Electronic address: justynak@chem.umk.pl.
authors.forename: J
authors.initials: J
authors.lastname: Kozłowska
authors.affiliation: Faculty of Chemistry, N. Copernicus University, Gagarin 7, 87-100 Torun, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sionkowska
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D051381:Rats; D012995:Solubility; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D003094:Collagen; C020243:calcium phosphate
keywords: Calcium phosphate; Collagen; Composite scaffolds