title: Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia.
abstract: BACKGROUND & OBJECTIVESI: Transovarial transmission of dengue virus in the Aedes vectors is now a well-documented phenomenon reported from many parts of the endemic areas in the world, which played an important role in initiating and maintaining the outbreak in human populations. This study investigated the factors affecting breeding habitats and the relationship with transovarial dengue virus in larvae of Aedes aegypti and Ae. albopictus.
  
  
  METHODS
  Larval surveillance was conducted in dengue outbreak areas in Malaysia from 2008 until 2009. Sampling was carried out based on habitat type, water condition (substrate type), canopy coverage, temperature and pH at breeding habitats. RT-PCR was performed to detect presence of transovarial dengue virus in larvae collected in the study areas.
  
  
  RESULTS
  A total of 789 breeding habitats were identified during this study and the majority of these breeding sites were plastic containers (57.46%). Aedes albopictus dominated most of the water condition surveyed, while Ae. aegypti indicated preference toward habitats with clear water. Aedes aegypti was selective in selecting ovipositional sites compared to Ae. albopictus where shaded areas were shown to be the most preferred. From a total of 363 mosquito larvae pools, 23 (6.3%) pools were positive for dengue virus where 18 of them were from Ae. albopictus and five were from Ae. aegypti mosquito larvae pools.
  
  
  CONCLUSIONS
  This study indicated the presence of transovarial transmission of dengue virus in immature Ae. aegypti and Ae. albopictus in the field. This study also showed that combination of water conditions, canopy coverage, temperature and pH of breeding habitats were the factors affecting the larval population. The study suggested that larval survey programme could serve as a tool not only to monitor the local dengue vector distribution but also to provide objective information for taking appropriate action by the community against dengue vectors.
authors.affiliation: Medical Entomology Unit, Infectious Disease Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia.
authors.forename: A
authors.initials: A
authors.lastname: Rohani
authors.forename: A R
authors.initials: AR
authors.lastname: Aidil Azahary
authors.forename: M
authors.initials: M
authors.lastname: Malinda
authors.forename: M N
authors.initials: MN
authors.lastname: Zurainee
authors.forename: H
authors.initials: H
authors.lastname: Rozilawati
authors.forename: W M A
authors.initials: WM
authors.lastname: Wan Najdah
authors.forename: H L
authors.initials: HL
authors.lastname: Lee
mesh_terms: D000330:Aedes / Q000254:growth & development* / Q000821:virology*; D000818:Animals; D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000302:isolation & purification*; D017753:Ecosystem; D007303:Insect Vectors; D007814:Larva / Q000254:growth & development / Q000821:virology; D008296:Malaysia / Q000453:epidemiology; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012367:RNA, Viral