title: The fate of suboptimal anastomosis after colon resection: An experimental study.
abstract: BACKGROUND
  The fate of suboptimal anastomosis is unknown and early detection of anastomotic leakage after colon resection is crucial for the proper management of patients.
  
  
  METHODS
  Twenty-six rats were assigned to "Control", "Leakage" and "Suboptimal anastomosis" groups where they underwent either sham laparotomy, cecal ligation, and puncture or anastomosis with four sutures following colon resection, respectively. At the fifth hour and on the third and ninth days; peripheral blood and peritoneal washing samples through relaparotomy were obtained. The abdomen was inspected macroscopically for anastomotic healing. Polymerase chain reaction (PCR) with 16s rRNA and E.coli-specific primers were run on all samples along with aerobic and anaerobic cultures.
  
  
  RESULTS
  The sensitivity and specificity of PCR on different bodily fluids with 16s rRNA and E.coli-specific primers were 100% and 78%, respectively. All samples of peritoneal washing fluids on the third and ninth days showed presence of bacteria in both PCR and culture. The inspection of the abdomen revealed signs of anastomotic leakage in eight rats (80%), whereas mortality related with anastomosis was detected in two (20%).
  
  
  CONCLUSIONS
  Anastomotic leakage with suboptimal anastomosis after colon resection is high and the early detection is possible by running PCR on peritoneal samples as early as 72 hours.
authors.affiliation: Department of General Surgery, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey. mehmetkamilyildiz@yahoo.com.
authors.forename: Mehmet Kamil
authors.initials: MK
authors.lastname: Yıldız
authors.affiliation: Department of General Surgery, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Okan
authors.affiliation: Department of Microbiology and Clinical Microbiology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Nazik
authors.affiliation: Department of Surgery, Ümraniye Training and Research Hospital, İstanbul, Turkey.
authors.forename: Gurhan
authors.initials: G
authors.lastname: Bas
authors.affiliation: Department of Surgery, Medeniyet University Göztepe Training and Research Hospital, İstanbul, Turkey.
authors.forename: Orhan
authors.initials: O
authors.lastname: Alimoglu
authors.affiliation: Department of Microbiology and Clinical Microbiology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: İlktac
authors.affiliation: Department of General Surgery, Vakıf Gureba Training and Research Hospital, İstanbul, Turkey.
authors.forename: Emin
authors.initials: E
authors.lastname: Daldal
authors.affiliation: Department of General Surgery, Gaziosmanpaşa University Faculty of Medicine, Tokat, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sahin
authors.affiliation: Department of Microbiology and Clinical Microbiology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.
authors.forename: Nuray
authors.initials: N
authors.lastname: Kuvat
authors.affiliation: Department of Microbiology and Clinical Microbiology, İstanbul University İstanbul Faculty of Medicine, İstanbul, Turkey.
authors.forename: Betugul
authors.initials: B
authors.lastname: Ongen
mesh_terms: D000714:Anastomosis, Surgical; D057868:Anastomotic Leak / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D000818:Animals; D016470:Bacteremia / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D003106:Colon / Q000382:microbiology / Q000601:surgery*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000302:isolation & purification; D008297:Male; D016133:Polymerase Chain Reaction; D011183:Postoperative Complications / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing
chemical_list: D012336:RNA, Ribosomal, 16S