title: Effect of Centella asiatica Leaf Extract on the Dietary Supplementation in Transgenic Drosophila Model of Parkinson's Disease.
abstract: The role of Centella asiatica L. leaf extract was studied on the transgenic Drosophila model flies expressing normal human alpha synuclein (h-αS) in the neurons. The leaf extract was prepared in acetone and was subjected to GC-MS analysis. C. asiatica extract at final concentration of 0.25, 0.50, and 1.0 μL/mL was mixed with the diet and the flies were allowed feeding on it for 24 days. The effect of extract was studied on the climbing ability, activity pattern, lipid peroxidation, protein carbonyl content, glutathione content, and glutathione-S-transferase activity in the brains of transgenic Drosophila. The exposure of extract to PD model flies results in a significant delay in the loss of climbing ability and activity pattern and reduced the oxidative stress (P < 0.05) in the brains of PD flies as compared to untreated PD flies. The results suggest that C. asiatica leaf extract is potent in reducing the PD symptoms in transgenic Drosophila model of Parkinson's disease.
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Yasir Hasan
authors.initials: YH
authors.lastname: Siddique
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Falaq
authors.initials: F
authors.lastname: Naz
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Jyoti
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Ambreen
authors.initials: A
authors.lastname: Fatima
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Saba
authors.initials: S
authors.lastname: Khanam
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.lastname: Rahul
authors.affiliation: Drosophila Transgenic Laboratory, Section of Genetics, Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India.
authors.forename: Fahad
authors.initials: F
authors.lastname: Ali
authors.affiliation: Photobiology Division, CSIR-Indian Institute of Toxicology Research, Lucknow, Uttar Pradesh 206002, India.
authors.forename: Syed Faiz
authors.initials: SF
authors.lastname: Mujtaba
authors.affiliation: Forest Entomology Division, Forest Research Institute, Dehradun 248006, India.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Faisal