title: Fermentative production of 1-propanol from sugars using wild-type and recombinant Shimwellia blattae.
abstract: Shimwellia blattae is an enteric bacterium and produces endogenous enzymes that convert 1,2-propanediol (1,2-PD) to 1-propanol, which is expected to be used as a fuel substitute and a precursor of polypropylene. Therefore, if S. blattae could be induced to generate its own 1,2-PD from sugars, it might be possible to produce 1-propanol from sugars with this microorganism. Here, two 1,2-PD production pathways were constructed in S. blattae, resulting in two methods for 1-propanol production with the bacterium. One method employed the L-rhamnose utilization pathway, in which L-rhamnose is split into dihydroxyacetone phosphate and 1,2-PD. When wild-type S. blattae was cultured with L-rhamnose, an accumulation of 1,2-PD was observed. The other method for producing 1,2-PD was to introduce an engineered 1,2-PD production pathway from glucose into S. blattae. In both cases, the produced 1,2-PD was then converted to 1-propanol by 1,2-PD converting enzymes, whose production was induced by the addition of glycerol.
authors.affiliation: Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, Sakai, Osaka, 599-8531, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Urano
authors.forename: Misaki
authors.initials: M
authors.lastname: Fujii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kaino
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Matsubara
authors.forename: Michihiko
authors.initials: M
authors.lastname: Kataoka
mesh_terms: D000433:1-Propanol / Q000378:metabolism*; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D004755:Enterobacteriaceae / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D060847:Metabolic Engineering; D019946:Propylene Glycol / Q000378:metabolism; D012210:Rhamnose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D019946:Propylene Glycol; D000433:1-Propanol; D005947:Glucose; D005990:Glycerol; D012210:Rhamnose