title: RDH13L, an enzyme responsible for the aldehyde-alcohol redox coupling reaction (AL-OL coupling reaction) to supply 11-cis retinal in the carp cone retinoid cycle.
abstract: Cone photoreceptors require effective pigment regeneration mechanisms to maintain their sensitivity in the light. Our previous studies in carp cones suggested the presence of an unconventional and very effective mechanism to produce 11-cis retinal, the necessary component in pigment regeneration. In this reaction (aldehyde-alcohol redox coupling reaction, AL-OL coupling reaction), formation of 11-cis retinal, i.e. oxidation of 11-cis retinol is coupled to reduction of an aldehyde at a 1:1 molar ratio without exogenous NADP(H) which is usually required in this kind of reaction. Here, we identified carp retinol dehydrogenase 13-like (RDH13L) as an enzyme catalyzing the AL-OL coupling reaction. RDH13L was partially purified from purified carp cones, identified as a candidate protein, and its AL-OL coupling activity was confirmed using recombinant RDH13L. We further examined the substrate specificity, subcellular localization, and expression level of RDH13L. Based on these results, we concluded that RDH13L contributes to a significant part, but not all, of the AL-OL coupling activity in carp cones. RDH13L contained tightly bound NADP(+) which presumably functions as a cofactor in the reaction. Mouse RDH14, a mouse homolog of carp RDH13L, also showed the AL-OL coupling activity. Interestingly, although carp cone membranes, carp RDH13L and mouse RDH14 all showed the coupling activity at 15-37 °C, they also showed a conventional NADP(+)-dependent 11-cis retinol oxidation activity above 25 °C without addition of aldehydes. This dual mechanism of 11-cis retinal synthesis attained by carp RDH13L and mouse RDH14 probably contribute to effective pigment regeneration in cones that function in the light.
authors.affiliation: From the Graduate School of Frontier Biosciences and.
authors.forename: Shinya
authors.initials: S
authors.lastname: Sato
authors.affiliation: From the Graduate School of Frontier Biosciences and.
authors.forename: Sadaharu
authors.initials: S
authors.lastname: Miyazono
authors.affiliation: From the Graduate School of Frontier Biosciences and Department of Biological Sciences, Graduate School of Science, Osaka University, Yamada-oka 1-3, Suita, Osaka 565-0871, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Tachibanaki
authors.affiliation: From the Graduate School of Frontier Biosciences and Department of Biological Sciences, Graduate School of Science, Osaka University, Yamada-oka 1-3, Suita, Osaka 565-0871, Japan kawamura@fbs.osaka-u.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kawamura
mesh_terms: D000818:Animals; D002347:Carps / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D017949:Retinal Cone Photoreceptor Cells / Q000201:enzymology* / Q000378:metabolism*; D012172:Retinaldehyde / Q000378:metabolism*; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D014801:Vitamin A; D012172:Retinaldehyde
keywords: NADP; Photoreceptor; Retinal; Retinal Metabolism; Retinoid; Retinol; Vision; Visual Cycle; Vitamin A