title: Role of Ctf3 and COMA subcomplexes in meiosis: Implication in maintaining Cse4 at the centromere and numeric spindle poles.
abstract: During mitosis and meiosis, kinetochore, a conserved multi-protein complex, connects microtubule with the centromere and promotes segregation of the chromosomes. In budding yeast, central kinetochore complex named Ctf19 has been implicated in various functions and is believed to be made up of three biochemically distinct subcomplexes: COMA, Ctf3 and Iml3-Chl4. In this study, we aimed to identify whether Ctf3 and COMA subcomplexes have any unshared function at the kinetochore. Our data suggests that both these subcomplexes may work as a single functional unit without any unique functions, which we tested. Analysis of severity of the defects in the mutants suggests that COMA is epistatic to Ctf3 subcomplex. Interestingly, we noticed that these subcomplexes affect the organization of mitotic and meiotic kinetochores with subtle differences and they promote maintenance of Cse4 at the centromeres specifically during meiosis which is similar to the role of Mis6 (Ctf3 homolog) in fission yeast during mitosis. Interestingly, analysis of ctf3Δ and ctf19Δ mutants revealed a novel role of Ctf19 complex in regulation of SPB cohesion and duplication in meiosis.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 40076, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Agarwal
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 40076, India.
authors.forename: Gunjan
authors.initials: G
authors.lastname: Mehta
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 40076, India. Electronic address: santanughosh@iitb.ac.in.
authors.forename: Santanu K
authors.initials: SK
authors.lastname: Ghosh
mesh_terms: D002503:Centromere / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018386:Kinetochores / Q000378:metabolism / Q000502:physiology*; D008540:Meiosis / Q000235:genetics; D046912:Multiprotein Complexes / Q000378:metabolism / Q000502:physiology*; D030781:Organisms, Genetically Modified; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism* / Q000502:physiology*; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D064047:Spindle Poles / Q000378:metabolism*
chemical_list: C092746:CSE4 protein, S cerevisiae; D002868:Chromosomal Proteins, Non-Histone; C444703:Ctf3 protein, S cerevisiae; D004268:DNA-Binding Proteins; D046912:Multiprotein Complexes; D029701:Saccharomyces cerevisiae Proteins
keywords: Budding yeast; Ctf19; Ctf3; Kinetochore; Meiosis; Spindle pole body