title: Function and subsets of dendritic cells and natural killer cells were decreased in gastric cancer.
abstract: Dendritic cells (DCs) and natural killer (NK) cells initiate specific immune responses against tumor cells. The aim of the present study was to determine the cytotoxicity and the subsets of the DC and NK cells and the cytokines level of DC and NK cells from cancer tissue and peripheral blood in the gastric cancer patients. Cytotoxicity of DC and NK was determined using the Cytotox non-radioactive assay. The cytotoxic activity of DC or NK isolated from cancer tissue and peripheral blood was attenuated in gastric cancer patients. CD11c, CD80, CD83, CD16, CD57 and CD69 were decreased in the cancer tissue and peripheral blood in the gastric cancer patients. CD86, CCR7 and CD59 were no significance in the cancer tissue and peripheral blood from gastric cancer patients. Tumor necrosis factor (TNF)-α, interleukin (IL)-2, T-bet and IL-15Rβ levels were decreased in DC and NK from the gastric cancer tissue and peripheral blood in the gastric cancer patients. IL-15 and IL-15Rα level were no significance in DC and NK in the gastric cancer tissue and peripheral blood in the gastric cancer patients. These results indicate that the cytotoxic activity and subsets and cytokines of DC and NK cells in the cancer tissue and peripheral blood in the gastric cancer patients were decreased. The decrease of subsets content and cytokines of DC and NK may contribute to a decrease in the function of DC and NK in the tissue and peripheral blood in the gastric cancer patients.
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
authors.forename: Jingting
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Department of Gastroenterology, The Third Affiliated Hospital of Soochow University, The First People's Hospital of Changzhou Changzhou, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D015153:Blotting, Western; D016207:Cytokines / Q000096:biosynthesis; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology; D013274:Stomach Neoplasms / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D016207:Cytokines
keywords: Dendritic cell; cytokine; cytotoxicity; natural killer cell