title: Two activities of long-chain acyl-coenzyme A synthetase are involved in lipid trafficking between the endoplasmic reticulum and the plastid in Arabidopsis.
abstract: In plants, fatty acids are synthesized within the plastid and need to be distributed to the different sites of lipid biosynthesis within the cell. Free fatty acids released from the plastid need to be converted to their corresponding coenzyme A thioesters to become metabolically available. This activation is mediated by long-chain acyl-coenzyme A synthetases (LACSs), which are encoded by a family of nine genes in Arabidopsis (Arabidopsis thaliana). So far, it has remained unclear which of the individual LACS activities are involved in making plastid-derived fatty acids available to cytoplasmic glycerolipid biosynthesis. Because of its unique localization at the outer envelope of plastids, LACS9 was regarded as a candidate for linking plastidial fatty export and cytoplasmic use. However, data presented in this study show that LACS9 is involved in fatty acid import into the plastid. The analyses of mutant lines revealed strongly overlapping functions of LACS4 and LACS9 in lipid trafficking from the endoplasmic reticulum to the plastid. In vivo labeling experiments with lacs4 lacs9 double mutants suggest strongly reduced synthesis of endoplasmic reticulum-derived lipid precursors, which are required for the biosynthesis of glycolipids in the plastids. In conjunction with this defect, double-mutant plants accumulate significant amounts of linoleic acid in leaf tissue.
authors.affiliation: Departments of Plant Biochemistry (D.J., M.F.) and Plant Cell Biology (C.R., M.W.), Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, 37077 Goettingen, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Jessen
authors.affiliation: Departments of Plant Biochemistry (D.J., M.F.) and Plant Cell Biology (C.R., M.W.), Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, 37077 Goettingen, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Roth
authors.affiliation: Departments of Plant Biochemistry (D.J., M.F.) and Plant Cell Biology (C.R., M.W.), Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, 37077 Goettingen, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Wiermer
authors.affiliation: Departments of Plant Biochemistry (D.J., M.F.) and Plant Cell Biology (C.R., M.W.), Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University, 37077 Goettingen, Germany mfulda@gwdg.de.
authors.forename: Martin
authors.identifier: http://orcid.org/0000-0001-5734-067X
authors.initials: M
authors.lastname: Fulda
mesh_terms: D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001692:Biological Transport; D003066:Coenzyme A Ligases / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004789:Enzyme Activation; D005227:Fatty Acids / Q000378:metabolism; D050356:Lipid Metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D018515:Plant Leaves / Q000378:metabolism; D010938:Plant Oils / Q000378:metabolism; D018087:Plastids / Q000378:metabolism*; D012098:Reproduction; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D029681:Arabidopsis Proteins; D005227:Fatty Acids; C000601796:LACS4 protein, Arabidopsis; D008563:Membrane Lipids; D010938:Plant Oils; D003066:Coenzyme A Ligases; C468564:LACS9 protein, Arabidopsis