title: Trichomonas vaginalis metalloproteinase induces mTOR cleavage of SiHa cells.
abstract: Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
authors.forename: Juan-Hua
authors.initials: JH
authors.lastname: Quan
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: In-Wook
authors.initials: IW
authors.lastname: Choi
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
authors.forename: Jung-Bo
authors.initials: JB
authors.lastname: Yang
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: Guang-Ho
authors.initials: GH
authors.lastname: Cha
authors.affiliation: Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College, Zhanjiang 524-001, Guangdong, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Environmental Biology and Medical Parasitology, Hanyang University College of Medicine, Seoul 133-791, Korea.
authors.forename: Jae-Sook
authors.initials: JS
authors.lastname: Ryu
authors.affiliation: Department of Infection Biology, Chungnam National University School of Medicine, Daejeon 301-131, Korea.
authors.forename: Young-Ha
authors.initials: YH
authors.lastname: Lee
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism / Q000469:parasitology; D006801:Humans; D045726:Metalloproteases / Q000235:genetics / Q000378:metabolism*; D059748:Proteolysis; D017422:Sequence Analysis, DNA; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism*; D014246:Trichomonas vaginalis / Q000201:enzymology* / Q000235:genetics
chemical_list: C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D045726:Metalloproteases
keywords: 1,10-phenanthroline; SiHa cell; Trichomonas vaginalis; mTOR cleavage; metalloproteinase