title: Production of a fragment of glycoprotein G of herpes simplex virus type 2 and evaluation of its diagnostic potential.
abstract: BACKGROUND
  Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit.
  
  
  METHODS
  We successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG(321-580His)) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG(321-580His) as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference.
  
  
  RESULTS
  In testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG(321-580His)-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%.
  
  
  CONCLUSIONS
  The study indicates that gG(321-580His) has a high diagnostic potential for HSV-2 virus serodiagnosis in humans.
authors.affiliation: Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, People's Republic of China.
authors.forename: Ji Feng
authors.initials: JF
authors.lastname: Liu
authors.affiliation: Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yu
authors.affiliation: Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors.forename: Guo Jing
authors.initials: GJ
authors.lastname: Si
authors.affiliation: Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hu
authors.affiliation: Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, Hangzhou, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006558:Herpes Genitalis / Q000175:diagnosis* / Q000821:virology; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008297:Male; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic
keywords: baculovirus expression system; glycoprotein G; herpes simplex virus type 2; immunodominant fragment; indirect enzyme-linked immunosorbent assay