title: Localization of ginsenosides in the rhizome and root of Panax ginseng by laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry.
abstract: The root and rhizome of Panax ginseng C.A. Mey, known as ginseng, is a commonly used medicinal plant. Ginsenosides are the major active components responsible for the tonic effects of this herb. Here, the combination of laser microdissection and ultra-high performance liquid chromatography quadrupole/time of flight-mass spectrometry (UHPLC-QTOF-MS) was applied to investigate the localization of ginsenosides in root and rhizome of P. ginseng. Five kinds of tissue cells were separated from the rhizome, main root and branch root of ginseng. Fifty-nine ginsenosides were identified and the results showed that the cork contained more kinds of ginsenosides than did the cortex, phloem, xylem and resin canals. It is interesting that the phloem, xylem and resin canals from branch root contained a greater number of ginsenosides than did from main root. This study provides solid evidence on the accumulation of ginsenosides in cork, cortex, phloem and xylem.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region. Electronic address: lzt23@hkbu.edu.hk.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Liang
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region; Department of Resources Science of Traditional Chinese Medicines, State Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Resources Science of Traditional Chinese Medicines, State Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Nanjing, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Qin
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region.
authors.forename: Hubiao
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region. Electronic address: zzzhao@hkbu.edu.hk.
authors.forename: Zhongzhen
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036145:Ginsenosides / Q000302:isolation & purification*; D061346:Laser Capture Microdissection / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry; D027343:Rhizome / Q000737:chemistry
chemical_list: D036145:Ginsenosides
keywords: Ginseng; Ginsenosides; Laser microdissection; Tissue-specific; UHPLC-Q/TOF-MS