title: The DET1-COP1-HY5 pathway constitutes a multipurpose signaling module regulating plant photomorphogenesis and thermomorphogenesis.
abstract: Developmental plasticity enables plants to respond to elevated ambient temperatures by adapting their shoot architecture. On the cellular level, the basic-helix-loop-helix (bHLH) transcription factor phytochrome interacting factor 4 (PIF4) coordinates this response by activating hormonal modules that in turn regulate growth. In addition to an unknown temperature-sensing mechanism, it is currently not understood how temperature regulates PIF4 activity. Using a forward genetic approach in Arabidopsis thaliana, we present extensive genetic evidence demonstrating that the de-etiolated 1 (DET1)-constitutive photomorphogenic 1 (COP1)-elongated hypocotyl 5 (HY5)-dependent photomorphogenesis pathway transcriptionally regulates PIF4 to coordinate seedling growth in response to elevated temperature. Our findings demonstrate that two of the most prevalent environmental cues, light and temperature, share a much larger set of signaling components than previously assumed. Similar to the toolbox concept in animal embryonic patterning, multipurpose signaling modules might have evolved in plants to translate various environmental stimuli into adaptational growth processes.
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Delker
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Louisa
authors.initials: L
authors.lastname: Sonntag
authors.affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
authors.forename: Geo Velikkakam
authors.initials: GV
authors.lastname: James
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Janitza
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Carla
authors.initials: C
authors.lastname: Ibañez
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Henriette
authors.initials: H
authors.lastname: Ziermann
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Peterson
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Denk
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Steffi
authors.initials: S
authors.lastname: Mull
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Ziegler
authors.affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany; Department of Biology, University of York, York YO10 5DD, UK.
authors.forename: Seth Jon
authors.initials: SJ
authors.lastname: Davis
authors.affiliation: Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany.
authors.forename: Korbinian
authors.initials: K
authors.lastname: Schneeberger
authors.affiliation: Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle, Germany. Electronic address: mquint@ipb-halle.de.
authors.forename: Marcel
authors.initials: M
authors.lastname: Quint
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D064527:Etiolation; D018506:Gene Expression Regulation, Plant; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D063246:Organogenesis, Plant; D015398:Signal Transduction; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; C088435:DET1 protein, Arabidopsis; C109254:HY5 protein, Arabidopsis; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases