title: Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects.
abstract: Loop-mediated isothermal amplification (LAMP), a newly developed gene amplification method, combines rapidity, simplicity, and high specificity. Several tests have been developed based on this method, and simplicity is maintained throughout all steps, from extraction of nucleic acids to detection of amplification. In the LAMP reaction, samples are amplified at a fixed temperature through a repetition of two types of elongation reactions occurring at the loop regions: self-elongation of templates from the stem loop structure formed at the 3'-terminal and the binding and elongation of new primers to the loop region. The LAMP reaction has a wide range of possible applications, including point-of-care testing, genetic testing in resource-poor settings (such as in developing countries), and rapid testing of food products and environmental samples.
authors.affiliation: Biochemical Research Laboratory, Research & Development Division, Eiken Chemical Co., Ltd. 1381-3 Shimoishigami, Ohtawara, Tochigi, 324-0036, Japan, Tsugunori_Notomi@eiken.co.jp.
authors.forename: Tsugunori
authors.initials: T
authors.lastname: Notomi
authors.forename: Yasuyoshi
authors.initials: Y
authors.lastname: Mori
authors.forename: Norihiro
authors.initials: N
authors.lastname: Tomita
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kanda
mesh_terms: D017931:DNA Primers; D005820:Genetic Testing; D006801:Humans; D055029:Inverted Repeat Sequences; D021141:Nucleic Acid Amplification Techniques; D009975:Orthomyxoviridae / Q000302:isolation & purification; D019095:Point-of-Care Systems; D013696:Temperature; D014376:Tuberculosis / Q000175:diagnosis
chemical_list: D017931:DNA Primers