title: Rapid quantification of resveratrol in mouse plasma by ultra high pressure liquid chromatography (UPLC) coupled to tandem mass spectrometry.
abstract: OBJECTIVE
  The objective of this study was to develop a rapid and sensitive method for the quantification of resveratrol, a polyphenolic compound with multiple health beneficial effects, in mouse plasma.
  
  
  METHODS
  We used reversed-phase ultra high pressure-liquid chromatography with tandem mass spectrometry detection for the determination of resveratrol levels in mouse plasma. An Agilent Zorbax Eclipse Plus C18 column (2.1 mm x 50 mm, 1.8 μm) was used as the stationary phase. The mobile phase consisted of a gradient formed using 1 mM ammonium fluoride and methanol.
  
  
  RESULTS
  Using this improved method, we obtained a retention time of 2.2 min and a total run time of 5 min, for resveratrol. The calibration curve for resveratrol showed a linear range from 0.5 to 100 ng/mL. The average coefficient of variation was 6% for interday variation and 4% for intraday variation. The recovery for resveratrol in mouse plasma was 85 ± 10% (mean ± standard deviation).
  
  
  CONCLUSIONS
  The method presented herein allows a rapid and very sensitive quantification of resveratrol in mouse plasma at concentrations as low as 500 ppt.
authors.affiliation: Department of Biochemistry, University of Puerto Rico, San Juan. linnettecastillo@yahoo.com
authors.forename: Linette
authors.initials: L
authors.lastname: Castillo-Pichardo
authors.forename: Suranganie
authors.initials: S
authors.lastname: Dharmawardhane
authors.forename: José F
authors.initials: JF
authors.lastname: Rodríguez-Orengo
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D051379:Mice / Q000097:blood*; D015394:Molecular Structure; D012015:Reference Standards; D000077185:Resveratrol; D012680:Sensitivity and Specificity; D013267:Stilbenes / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013267:Stilbenes; D000077185:Resveratrol