title: Optimization of a high-resolution radical scavenging assay coupled on-line to reversed-phase liquid chromatography for antioxidant detection in complex natural extracts.
abstract: This paper reports the optimization of the on-line coupling of 2,2'-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid based radical scavenging assays with reversed-phase high-performance liquid chromatography. The residence time in the reactor was reduced to 6.4 s to ensure minimal peak broadening and loss of separation. Peak capacity losses between compound detection and measurement of the radical scavenging potential were reduced to 10% and lower on coupled column systems. The methodology was successfully applied for the detection of the scavenging activity of molecules encompassing a broad hydrophobicity range. The method shows promise for the assessment of low-molecular-weight polyphenols in red wine by coupled-column high-resolution high-performance liquid chromatography with mass spectrometry analysis.
authors.affiliation: Separation Science Group, Department of Organic & Macromolecular Chemistry, Ghent University, Ghent, Belgium.
authors.forename: Seppe
authors.initials: S
authors.lastname: De Smet
authors.forename: Bram
authors.initials: B
authors.lastname: Miserez
authors.forename: Maria
authors.initials: M
authors.lastname: Rambla Alegre
authors.forename: Mehmet
authors.initials: M
authors.lastname: Talha Yapa
authors.forename: André
authors.initials: A
authors.lastname: de Villiers
authors.forename: Pat
authors.initials: P
authors.lastname: Sandra
authors.forename: Frederic
authors.initials: F
authors.lastname: Lynen
keywords: Antioxidants; High-performance liquid chromatography; Radicals; Wine