title: Transcriptome and proteome analysis of Eucalyptus infected with Calonectria pseudoreteaudii.
abstract: Cylindrocladium leaf blight is one of the most severe diseases in Eucalyptus plantations and nurseries. There are Eucalyptus cultivars with resistance to the disease. However, little is known about the defense mechanism of resistant cultivars. Here, we investigated the transcriptome and proteome of Eucalyptus leaves (E. urophylla×E. tereticornis M1), infected or not with Calonectria pseudoreteaudii. A total of 8585 differentially expressed genes (|log2 ratio| ≥1, FDR ≤0.001) at 12 and 24hours post-inoculation were detected using RNA-seq. Transcriptional changes for five genes were further confirmed by qRT-PCR. A total of 3680 proteins at the two time points were identified using iTRAQ technique.The combined transcriptome and proteome analysis revealed that the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway (jasmonic acid and sugar) were activated. The data also showed that some proteins (WRKY33 and PR proteins) which have been reported to involve in plant defense response were up-regulated. However, photosynthesis, nucleic acid metabolism and protein metabolism were impaired by the infection of C. pseudoreteaudii. This work will facilitate the identification of defense related genes and provide insights into Eucalyptus defense responses to Cylindrocladium leaf blight.
  
  
  CONCLUSIONS
  In this study, a total of 130 proteins and genes involved in the shikimate/phenylpropanoid pathway, terpenoid biosynthesis, signalling pathway, cell transport, carbohydrate and energy metabolism, nucleic acid metabolism and protein metabolism in Eucalyptus leaves after infected with C. pseudoreteaudii were identified. This is the first report of a comprehensive transcriptomic and proteomic analysis of Eucalyptus in response to Calonectria sp.
authors.affiliation: Sanming City Forest Disease and Pest Control and Quarantine Station, Sanming, Fujian 365000, China.
authors.forename: Quanzhu
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: fjgws@126.com.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Guo
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Feng
authors.affiliation: Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Ye
authors.affiliation: Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Wanfeng
authors.initials: W
authors.lastname: Xie
authors.affiliation: Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Huang
authors.affiliation: Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Liu
mesh_terms: D001203:Ascomycota / Q000378:metabolism*; D005052:Eucalyptus / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism* / Q000382:microbiology; D010940:Plant Proteins / Q000096:biosynthesis*; D020543:Proteome / Q000096:biosynthesis*; D040901:Proteomics; D059467:Transcriptome
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: Cylindrocladium leaf blight; Defense pathways; Defense related gene; Eucalyptus; RNA-seq; iTRAQ