title: A Ca(2+) concentration of 1.5 mM, as present in IMDM but not in RPMI, is critical for maximal response of Th cells to PMA/ionomycin.
authors.affiliation: Cell Biology Group, Deutsches Rheumaforschungszentrum, Berlin, Germany, a Leibniz Institute.
authors.forename: Jakob
authors.initials: J
authors.lastname: Zimmermann
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
authors.forename: Hyun-Dong
authors.initials: HD
authors.lastname: Chang
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis / Q000494:pharmacology*; D018929:Cell Culture Techniques; D003092:Colitis / Q000276:immunology; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis*; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology
chemical_list: D003470:Culture Media; D016207:Cytokines; D015759:Ionomycin; D002118:Calcium
keywords: CD4+ T cells; Cellular activation; Cytokines; Flow cytometry; PMA/ionomycin