title: Factors influencing elevated serum apolipoprotein B48 in diabetic and control participants.
abstract: Factors influencing the concentration of apolipoprotein B48 (apo B48) at fasting and post-prandial time frames are still being elucidated. This study assesses some possible contributing factors including the presence of type 2 diabetes and gender using an established enzyme-linked immunosorbent assay (ELISA) method. Apo B48 and triglyceride (TG) levels were measured before and for two, four and six hours post-prandially in 49 poorly controlled participants with type 2 diabetes and in 60 apparently healthy participants (controls). Apo B48 levels in the control participants increased post-prandially, peaking at four hours (14.81 ± 7.72 μg/mL) with similar responses demonstrated in TG concentrations. Post-prandial apo B48 levels were significantly higher in male control participants as demonstrated by apo B48 area under the curve (AUC); similar responses were also confirmed in triglyceride AUC. Post-prandial apo B48 concentrations in control participants correlated with HOMA-IR (P < 0.05). Apo B48 continued to increase throughout the six hours in participants with type 2 diabetes (17.73 ± 13.46 μg/mL), when levels were significantly greater than in the control participants (13.04 ± 7.67 μg/mL) (P < 0.05) despite a decrease in accompanying TG levels in participants with type 2 diabetes. Using an ELISA method, this study demonstrated that gender, insulin resistance (as evidenced by HOMA-IR) and diabetes status influence serum apo B48 levels. These effects were only apparent post-prandially.
authors.affiliation: Department of Clinical Biochemistry, Adelaide and Meath Hospital Dublin, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Leonard
authors.forename: T Kyaw
authors.initials: TK
authors.lastname: Tun
authors.forename: R
authors.initials: R
authors.lastname: Gaffney
authors.forename: J
authors.initials: J
authors.lastname: Sharma
authors.forename: J
authors.initials: J
authors.lastname: Gibney
authors.forename: G
authors.initials: G
authors.lastname: Boran
mesh_terms: D053283:Apolipoprotein B-48 / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood*
chemical_list: D053283:Apolipoprotein B-48; D014280:Triglycerides