title: Verification of a canine PSMA (FolH1) antibody.
abstract: BACKGROUND
  Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC is currently not available for dogs as data on specific canine PSMA detection are contradictory.
  
  
  METHODS
  To evaluate an antibody for specific canine PSMA detection by western blotting (WB), lysates of three canine prostatic cell lines (CT1258, DT08/40, DT08/46) were comparatively analyzed by WB and mass spectrometry (MS) to the human cell lines VCaP, LnCaP and PC-3.
  
  
  RESULTS
  MS analyses of the detected canine proteins confirmed cross reactivity of the antibody clone YPSMA-1 with canine PSMA.
  
  
  CONCLUSIONS
  The MS analyses of the extracted canine protein bands proved that the YPSMA-1 clone is as well specific for canine PSMA in WB and, thus, represents a reliable tool for comparative PSMA studies.
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Wagner
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany.
authors.forename: Denise
authors.initials: D
authors.lastname: Maibaum
authors.affiliation: Core Facility Proteomics, Hannover Medical School, Hannover, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Pich
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany ingo.nolte@tiho-hannover.de.
authors.forename: Ingo
authors.initials: I
authors.lastname: Nolte
authors.affiliation: Small Animal Clinic, University of Veterinary Medicine Hannover, Hannover, Germany Division of Medicine, Department of Haematology/Oncology, University of Rostock, Rostock, Germany.
authors.forename: Hugo
authors.initials: H
authors.lastname: Murua Escobar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000175:diagnosis / Q000378:metabolism*; D004285:Dogs; D043425:Glutamate Carboxypeptidase II / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; C473848:FOLH1 protein, human; D043425:Glutamate Carboxypeptidase II
keywords: PSMA; Prostate cancer; antibody cross-reactivity; diagnosis; dog; molecular marker