title: Single electrode biosensor for simultaneous determination of interferon gamma and lysozyme.
abstract: Simultaneous detection of multiple biomarkers holds great promise for acute leukemia evaluation. Here, a novel biosensor is developed for simultaneous electrochemical detection of interferon gamma (IFN-γ) and lysozyme (Lys) based on aptamer recognition by coupling "signal-on" and "signal-off" modes. On one Au electrode, two kinds of signaling probes labeled by the thiolated ferrocene (Fc)- and methy blue (MB)- were designed to hybridize with IFN-γ and Lys aptamers respectively to form partial complementary DNA duplexes. In the presence of IFN-γ and Lys, the target-aptamer interaction led to the release of aptamer from duplex DNA structure. The single-stranded signaling probes thus suffered from the conformation changes, which resulted in the decreased (or increased) oxidation peak current of Fc (or MB) according to the "signal-off (or signal-on)" mode. Electrodes were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, the signal changes were quantified using square wave voltammetry (SWV). This proposed biosensor for IFN-γ and Lys possessed linear detection range from 0.01 to 10 nM and 0.1 to 100 nM, with the detection limits of 1.14×10(-3) nM and 0.0164 nM, respectively. Moreover, this biosensor was readily regenerated and proved successful toward the practical analysis. The proposed strategy could provide more integrated and reliable information for acute leukemia evaluation.
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Daimin
authors.initials: D
authors.lastname: Song
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Rijun
authors.initials: R
authors.lastname: Gui
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Bi
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Fiber Materials and Modern Textile, The Growing Base for State Key Laboratory, College of Chemical Science and Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Collaborative Innovation Center for Marine Biomass Fiber Materials and Textiles, Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Linhua
authors.initials: L
authors.lastname: Xia
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D005296:Ferrous Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007371:Interferon-gamma / Q000737:chemistry / Q000302:isolation & purification*; D007938:Leukemia / Q000175:diagnosis*; D000075163:Metallocenes; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D005296:Ferrous Compounds; D000075163:Metallocenes; D009841:Oligonucleotides; D006046:Gold; D007371:Interferon-gamma; D009113:Muramidase; C004998:ferrocene
keywords: Biosensor; Electrochemical; Interferon gamma; Lysozyme; Signal-off; Signal-on