title: Rhizoctonia bataticola lectin (RBL) induces phenotypic and functional characteristics of macrophages in THP-1 cells and human monocytes.
abstract: We have previously reported that a fungal lectin, Rhizoctonia bataticola lectin (RBL), stimulates proliferation and secretion of Th1/Th2 cytokines in human peripheral blood mononuclear cells (PBMC). In the present study, we evaluated the ability of RBL to differentiate human monocytes to macrophages. RBL induced morphological changes indicative of differentiation in primary monocytes and THP-1 cells. Stimulation with RBL resulted in significant up-regulation of differentiation markers - CD54, HLA-DR, CD11b and CD11c and secretion of proinflammatory cytokines - IL-1β, TNF-α and IL-6. Functionally, RBL profoundly increased phagocytic activity in monocytes. In THP-1 cells, RBL-induced phagocytosis was higher compared to the effect induced by combination of phorbol-12-myristate-13-acetate (PMA) and lipopolysaccharide (LPS). RBL induced a significant increase in matrix metalloproteinase-9 (MMP-9) activity in comparison with a combined treatment of PMA+LPS. Mechanistic studies revealed the involvement of the NF-κB pathway in RBL-induced differentiation of monocytes. The data suggest that RBL mimics the combined action of PMA and LPS to induce morphological and functional differentiation in human monocytes and monocytic cell line - THP-1 to macrophages. Human monocytes differentiated to macrophages with RBL have the potential as an in vitro model to study macrophage biology.
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Pune 411007, Maharashtra, India.
authors.forename: Radha
authors.initials: R
authors.lastname: Pujari
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Pune 411007, Maharashtra, India.
authors.forename: Natesh
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: Suhas
authors.initials: S
authors.lastname: Ballal
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: Sachin M
authors.initials: SM
authors.lastname: Eligar
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: S
authors.initials: S
authors.lastname: Anupama
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: Ganapati
authors.initials: G
authors.lastname: Bhat
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: Bale M
authors.initials: BM
authors.lastname: Swamy
authors.affiliation: Department of Biochemistry, Karnatak University, Dharwad 580003, Karnataka, India.
authors.forename: Shashikala R
authors.initials: SR
authors.lastname: Inamdar
authors.affiliation: National Centre for Cell Science, Savitribai Phule Pune University Campus, Pune 411007, Maharashtra, India. Electronic address: padma@nccs.res.in.
authors.forename: Padma
authors.initials: P
authors.lastname: Shastry
mesh_terms: D001667:Binding, Competitive; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D018613:Microscopy, Confocal; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D012232:Rhizoctonia / Q000737:chemistry*; D013450:Sulfones / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: C434003:3-(4-methylphenylsulfonyl)-2-propenenitrile; D016207:Cytokines; D037102:Lectins; D008070:Lipopolysaccharides; D016328:NF-kappa B; D009570:Nitriles; D013450:Sulfones; D018799:Intercellular Adhesion Molecule-1; D020780:Matrix Metalloproteinase 9; D013755:Tetradecanoylphorbol Acetate
keywords: Differentiation; Fungal lectin; Macrophage; Monocyte; Phagocytosis