title: Adsorption properties of an activated carbon for 18 cytokines and HMGB1 from inflammatory model plasma.
abstract: The ability of an activated carbon (AC) to adsorb 18 different cytokines with molecular weights ranging from 8 kDa to 70 kDa and high mobility group box-1 (HMGB1) from inflammatory model plasma at 310 K and the mechanisms of adsorption were examined. Porosity analysis using N2 gas adsorption at 77K showed that the AC had micropores with diameters of 1-2 nm and mesopores with diameters of 5-20 nm. All 18 cytokines and HMGB1 were adsorbed on the AC; however, the shapes of the adsorption isotherms changed depending on the molecular weight. The adsorption isotherms for molecules of 8-10 kDa, 10-20 kDa, 20-30 kDa, and higher molecular weights were classified as H-2, L-3, S-3, and S-1 types, respectively. These results suggested that the adsorption mechanism for the cytokines and HMGB1 in the mesopores and on the surface of the AC differed as a function of the molecular weight. On the basis of these results, it can be concluded that AC should be efficient for cytokine adsorption.
authors.affiliation: Medical Material Laboratory, Asahi-Kasei Medical Co., Ltd., 2111-2, Oaza-sato, Oita 870-0396, Japan; Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Medical Material Laboratory, Asahi-Kasei Medical Co., Ltd., 2111-2, Oaza-sato, Oita 870-0396, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kiriyama
authors.affiliation: Medical Material Laboratory, Asahi-Kasei Medical Co., Ltd., 2111-2, Oaza-sato, Oita 870-0396, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Hatanaka
authors.affiliation: Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage, Chiba 263-8522, Japan. Electronic address: kanoh@faculty.chiba-u.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Kanoh
mesh_terms: D000327:Adsorption; D002244:Carbon / Q000097:blood / Q000737:chemistry*; D016207:Cytokines / Q000097:blood / Q000737:chemistry*; D024243:HMGB1 Protein / Q000097:blood / Q000737:chemistry*; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000097:blood*; D008954:Models, Biological; D008970:Molecular Weight; D010316:Particle Size; D013499:Surface Properties
chemical_list: D016207:Cytokines; D024243:HMGB1 Protein; C585491:HMGB1 protein, human; D002244:Carbon
keywords: Activated carbon; Adsorption; Cytokine; Inflammatory model plasma; Mesopore