title: Differential effects of peripheral and transitional prostatic stromal cells on tumorigenesis.
abstract: The human prostate contains two types of stromal cells, peripheral stromal cells (PSCs) and transitional stromal cells (TSCs). Here, we demonstrate the effects of PSCs and TSCs on tumorigenesis in prostate cancer (PCa) and identify the mechanisms underlying these effects. Using microarray analysis, we identified 3,643 differentially expressed genes in cocultures of TSCs, PSCs, and DU145 cells, a human prostate cancer cell line. Expression of cell division cycle 25 homolog A (CDC25A) was lower and that of tumor-associated calcium signal transducer 2 (TACSTD2) was higher in TSCs than in PSCs. Additionally, increased CDC25A expression or decreased TACSTD2 expression modulated the survival, growth, and migration of DU145 cells. These data suggest that PSCs promote and TSCs inhibit tumorigenesis by regulating the expression of CDC25A and TACSTD2.
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Bao
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Yu-Bing
authors.initials: YB
authors.lastname: Peng
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Wen-Ji
authors.initials: WJ
authors.lastname: Li
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Jun
authors.initials: J
authors.lastname: Da
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Urology, Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000328:Adult; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics; D001483:Base Sequence; D063646:Carcinogenesis; D015815:Cell Adhesion Molecules / Q000235:genetics; D002478:Cells, Cultured; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D011467:Prostate / Q000166:cytology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology*; D055815:Young Adult; D020687:cdc25 Phosphatases / Q000235:genetics
chemical_list: D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D017931:DNA Primers; D018076:DNA, Complementary; C499380:TACSTD2 protein, human; C517004:CDC25A protein, human; D020687:cdc25 Phosphatases