title: [Preliminary study of a universal vaccine based on the HA2 protein of the H5N1 influenza virus].
abstract: Fragments encoding amino acids 76-130 in the linear conserved region (LCR) of A/Hubei/1/2010 (H5N1) HA2 was fused to hepatitis B core antigen (HBc) to generate a LCR-HBe virus-like particle (VLP). Results showed that the fusion protein of LCR-HBc was highly expressed in this prokaryotic expression system. The purified LCR-HBc particle stimulated high levels of IgG production in mice with a titer of > 1:12 800, and provided 50% cross-protection against lethal challenge by H1N1 viruses.
authors.affiliation: World Health organization Collaborating Center for Reference and Research on Influenza, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xin
authors.forename: Xing-Yu
authors.initials: XY
authors.lastname: Yang
authors.forename: Zai-Jiang
authors.initials: ZJ
authors.lastname: Yu
authors.forename: Hong
authors.initials: H
authors.lastname: Bo
authors.forename: Jian-Fang
authors.initials: JF
authors.lastname: Zhou
authors.forename: Kun
authors.initials: K
authors.lastname: Qin
authors.forename: Yue-Long
authors.initials: YL
authors.lastname: Shu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology*; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D007371:Interferon-gamma / Q000096:biosynthesis; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D007371:Interferon-gamma