title: Co-administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 with prebiotic inulin alleviates the intestinal inflammation in rats exposed to N,N-dimethylhydrazine.
abstract: The aim of this study was to determine the anti-inflammatory effects of preventive administration of a probiotic strain Lactobacillus plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin or with flax-seed oil in the gut of rats, which developed chronic inflammation following administration of the pro-carcinogen N,N-dimethylhydrazine (DMH). After 28weeks administration of probiotic/prebiotic-containing diet, rats were killed and their colons were examined by immunohistological criteria, whereas cytokines were determined in the jejunal mucosa. Application of DMH triggered the production of pro-inflammatory cytokines IL-2, IL-6, IL-17, and TNF-α, expression of pro-inflammatory mediators NF-κB, COX-2 and iNOS and caused depletion of goblet cells. Supplementing the diet with L. plantarum and its combination with the prebiotic abolished DMH-induced inflammatory process in the jejunal mucosa by inhibiting the production of pro-inflammatory cytokines and by stimulation of anti-inflammatory IL-10 cytokine synthesis, whereas concentration of TGF-β1 was not influenced significantly. Diet prevented a decrease in goblet cell numbers but numbers of mast cells were lowered only moderately. However, combined treatment of rats with L. plantarum and flax-seed oil had no significant effect on the parameters examined, except for decreased expression of NF-κB, in comparison with the negative control. Results indicate that the preventive administration of probiotic L. plantarum LS/07 CCM7766 alone or in combination with prebiotic inulin to rats with DMH-induced chronic inflammation can reduce inflammatory process in the jejunal and colon mucosa, probably indirectly, and involves down-regulation of synthesis of pro-inflammatory cytokines and suppression of NF-κB activity in mucosal cells.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Štofilová
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Viktória
authors.initials: V
authors.lastname: Szabadosová
authors.affiliation: Department of Experimental Pharmacology, Institute of Parasitology of the Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovak Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hrčková
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Rastislav
authors.initials: R
authors.lastname: Salaj
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Izabela
authors.initials: I
authors.lastname: Bertková
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Emília
authors.initials: E
authors.lastname: Hijová
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Strojný
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of P.J. Šafárik, Trieda SNP 1, 04011 Košice, Slovak Republic.
authors.forename: Alojz
authors.initials: A
authors.lastname: Bomba
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D002273:Carcinogens; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051546:Cyclooxygenase 2 / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004127:Dimethylhydrazines; D005260:Female; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007410:Intestinal Diseases / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007444:Inulin / Q000494:pharmacology / Q000627:therapeutic use*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism; D048191:Lactiplantibacillus plantarum; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D056692:Prebiotics; D019936:Probiotics / Q000494:pharmacology / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D002273:Carcinogens; D016207:Cytokines; D004127:Dimethylhydrazines; D016328:NF-kappa B; D056692:Prebiotics; C013958:dimazine; D007444:Inulin; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat
keywords: Cancer prevention; Chronic inflammation; Flax-seed oil; Gut immunity; Inulin; Lactobacillus plantarum