title: Ozone inhalation modifies the rat liver proteome.
abstract: Ozone (O3) is a serious public health concern. Recent findings indicate that the damaging health effects of O3 extend to multiple systemic organ systems. Herein, we hypothesize that O3 inhalation will cause downstream alterations to the liver. To test this, male Sprague-Dawley rats were exposed to 0.5ppm O3 for 8h/day for 5 days. Plasma liver enzyme measurements showed that 5 day O3 exposure did not cause liver cell death. Proteomic and mass spectrometry analysis identified 10 proteins in the liver that were significantly altered in abundance following short-term O3 exposure and these included several stress responsive proteins. Glucose-regulated protein 78 and protein disulfide isomerase increased, whereas glutathione S-transferase M1 was significantly decreased by O3 inhalation. In contrast, no significant changes were detected for the stress response protein heme oxygenase-1 or cytochrome P450 2E1 and 2B in liver of O3 exposed rats compared to controls. In summary, these results show that an environmentally-relevant exposure to inhaled O3 can alter the expression of select proteins in the liver. We propose that O3 inhalation may represent an important unrecognized factor that can modulate hepatic metabolic functions.
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: wtheis01@uab.edu.
authors.forename: Whitney S
authors.initials: WS
authors.lastname: Theis
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: andringa@uab.edu.
authors.forename: Kelly K
authors.initials: KK
authors.lastname: Andringa
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA; Department of Pathology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: tmswain@uab.edu.
authors.forename: Telisha
authors.initials: T
authors.lastname: Millender-Swain
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA; Center for Free Radical Biology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: dadickin@uab.edu.
authors.forename: Dale A
authors.initials: DA
authors.lastname: Dickinson
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA; Center for Free Radical Biology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: epost@uab.edu.
authors.forename: Edward M
authors.initials: EM
authors.lastname: Postlethwait
authors.affiliation: Department of Environmental Health Sciences, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA; Department of Pathology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA; Center for Free Radical Biology, University of Alabama at Birmingham, 1720 2nd Avenue South, Birmingham, AL 35294, USA. Electronic address: sbailey@uab.edu.
authors.forename: Shannon M
authors.initials: SM
authors.lastname: Bailey
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010126:Ozone / Q000008:administration & dosage* / Q000494:pharmacology*; D020543:Proteome / Q000187:drug effects* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020543:Proteome; D010126:Ozone
keywords: Cytochrome P450; Endoplasmic reticulum stress; Liver; Ozone; Proteome; Stress response proteins