title: Transcriptomic response of the hydrothermal mussel Bathymodiolus azoricus in experimental exposure to heavy metals is modulated by the Pgm genotype and symbiont content.
abstract: Hydrothermal vent mussels belonging to the genus Bathymodiolus dominate communities at hydrothermal sites of the Mid-Atlantic Ridge. The mussel Bathymodiolus azoricus harbors thiotrophic and methanotrophic symbiotic bacteria in its gills and evolves in naturally highly metal contaminated environments. In the context of investigations on metal tolerance/effect in B. azoricus, we focused our work on the short-term adaptive response (15days) of mussels to different metals exposure at a molecular level using metal concentrations chosen to mimic natural situations at three vents sites. The expression of a set of 38 genes involved in different steps of the metal uptake, detoxication and various metabolisms was analysed by qPCR. Mussels were also genotyped at 10 enzyme loci to explore the relationships among natural genetic variation and gene expression. Relation between symbiont content (both sulfur-oxidizing and methanogen bacteria) and gene expression was also analysed. Our study demonstrated the influence of metal cocktail composition and time exposure on the transcriptome regulation with a specific pattern of regulation observed for the three metal cocktail tested. We also evidenced the significant influence of some specific Pgm genotype on the global gene expression in our experimental populations and a general trend of a higher gene expression in individuals carrying a high symbiont content.
authors.affiliation: CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France; CNRS/MNHN, UMR 7221, Evolution des Régulations Endocriniennes, MNHN, 7 Rue Cuvier, 75231 Paris Cedex 05, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Bougerol
authors.affiliation: CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Boutet
authors.affiliation: CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: LeGuen
authors.affiliation: CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Jollivet
authors.affiliation: CNRS, UMR 7144, Adaptation et Diversité en Milieu Marin, Station Biologique de Roscoff, 29680 Roscoff, France; Sorbonne Universités, UPMC Univ Paris 06, Station Biologique de Roscoff, 29680 Roscoff, France. Electronic address: atanguy@sb-roscoff.fr.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Tanguy
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000378:metabolism*; D049872:Bivalvia / Q000145:classification* / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005838:Genotype; D005880:Gills / Q000382:microbiology; D060086:Hydrothermal Vents; D019216:Metals, Heavy / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013559:Symbiosis; D059467:Transcriptome
chemical_list: D019216:Metals, Heavy; D012333:RNA, Messenger
keywords: Bathymodiolus azoricus; Genotype; Heavy metals stress; Symbiosis; Transcriptomic