title: miR-744 is a potential prognostic marker in patients with hepatocellular carcinoma.
abstract: Identification of new biomarkers for aggressiveness of hepatocellular carcinoma (HCC) to supplement the current group of prognosis algorithms is a significant clinical need. To clarify expression levels of microRNA-744 (miR-744) in HCC tissues and to explore its clinicopathological significance in HCC patients following liver transplantation (LT), we quantified miR-744 using real-time quantitative reverse transcription polymerase chain reaction in 96 paired cancerous tissues and para-cancerous normal liver tissues. We investigated relationships among miR-744 expression, clinicopathological parameters, and overall survival (OS). Of 96 paired samples, 68 cancer tissues expressed low miR-744 compared with their matched normal liver tissues. Patients with microvascular invasion or multi-tumor nodules showed significantly lower miR-744 expression; miR-744 was further decreased in patients with post-LT HCC recurrence compared with non-recurring patients. Patients with lower miR-744 expression showed significantly poorer recurrence-free survival and OS than individuals with higher miR-744 levels. Multivariate analysis revealed that lower miR-744 was an independent predictor of poor prognosis. Our results associate decreased miR-744 expression with HCC recurrence and prognosis, and also suggest that miR-744 is an independent predictor of survival in HCC patients after LT and may therefore be a potential biomarker for their prognosis.
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Yu-Le
authors.initials: YL
authors.lastname: Tan
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Bai
authors.affiliation: Organ Transplantation Institute, the General Hospital of Chinese People's Armed Police Forces, Beijing 100039 China.
authors.forename: Wei-Long
authors.initials: WL
authors.lastname: Zou
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Ma
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.forename: Jiang-She
authors.initials: JS
authors.lastname: Li
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
authors.lastname: Jie-Yin
authors.affiliation: Organ Transplantation Institute, the General Hospital of Chinese People's Armed Police Forces, Beijing 100039 China. Electronic address: zangyunjin@gmail.com.
authors.forename: Yun-Jin
authors.initials: YJ
authors.lastname: Zang
authors.affiliation: Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China. Electronic address: zhangzht@medmail.com.cn.
authors.forename: Zhong-Tao
authors.initials: ZT
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D016031:Liver Transplantation; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis
chemical_list: C569733:MIRN744 microRNA, human; D035683:MicroRNAs