title: Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE.
abstract: We identified and characterized a new eukaryotic-type protein kinase (PkaE) from Streptomyces coelicolor A3 (2) M145. PkaE, consisting of 510 amino acid residues, is a cytoplasmic protein kinase and contains the catalytic domain of eukaryotic protein kinases in the N-terminal region. Recombinant PkaE was found to be autophosphorylated at threonine residues only. The disruption of chromosomal pkaE resulted in the overproduction of the actinorhodin-related blue pigment antibiotics. pkaE was expressed during the late growth phase in S. coelicolor A3 (2) M145, which corresponded to the production time of blue pigments. This result indicated that PkaE acts as a negative regulator for production of the secondary metabolites. In addition, PkaE was able to phosphorylate KbpA, a regulator involved in the AfsK-AfsR regulatory pathway.
authors.affiliation: a Department of Biochemistry , Meiji Pharmaceutical University , Kiyose , Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Urabe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ogawara
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Motojima
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D047908:Intracellular Signaling Peptides and Proteins; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C436303:KbpA protein, Steptomyces coelicolor; D011994:Recombinant Proteins; D017346:Protein Serine-Threonine Kinases
keywords: Streptomyces coelicolor; secondary metabolism; serine/threonine protein kinase