title: Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst.
abstract: Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30°C. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition.
authors.affiliation: Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: In Yeub
authors.initials: IY
authors.lastname: Hwang
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Hur
authors.forename: Jae Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Park
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Eun Yeol
authors.initials: EY
authors.lastname: Lee
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D018533:Biomass; D003300:Copper / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D000432:Methanol / Q000378:metabolism*; D020602:Methylosinus trichosporium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005561:Formates; C030544:formic acid; D003300:Copper; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor); D008697:Methane; D000432:Methanol