title: Anxiety-like behaviour and c-fos expression in rats that inhaled vetiver essential oil.
abstract: Vetiver essential oil (VEO) has been used in aromatherapy for relaxation. This study aimed to investigate the effects of VEO on an anxiety-related behavioural model (the elevated plus-maze, EPM) and immediate-early gene c-fos in amygdala, known to be involved in anxiety. Male Wistar rats were administered diazepam (1 mg/kg i.p.) for 30 min or inhalated with VEO (1%, 2.5% or 5% w/w) for 7 min prior to exposure to the EPM. Then, the effects of 2.5% VEO, the anxiolytic dose, on c-fos expression in amygdala were investigated. The rats given either 2.5% VEO or diazepam exhibited an anxiolytic-like profile in the EPM. VEO and diazepam significantly increased c-fos expression in the lateral division of the central amygdaloid nucleus (CeL). Therefore, the anxiolytic properties of VEO might be associated with altering neuronal activation in CeL. However, future studies are needed to investigate the precise mechanism of action of VEO.
authors.affiliation: a Department of Physiology , Faculty of Medicine, Srinakharinwirot University , Bangkok , Thailand.
authors.forename: Somrudee
authors.initials: S
authors.lastname: Saiyudthong
authors.affiliation: b Department of Anatomy , Faculty of Medicine, Srinakharinwirot University , Bangkok , Thailand.
authors.forename: Sirinun
authors.initials: S
authors.lastname: Pongmayteegul
authors.affiliation: c School of Biomedical Sciences, University of Nottingham , Nottingham , UK.
authors.forename: Charles A
authors.initials: CA
authors.lastname: Marsden
authors.affiliation: b Department of Anatomy , Faculty of Medicine, Srinakharinwirot University , Bangkok , Thailand.
authors.forename: Pansiri
authors.initials: P
authors.lastname: Phansuwan-Pujito
mesh_terms: D000280:Administration, Inhalation; D000679:Amygdala / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D001007:Anxiety / Q000188:drug therapy*; D019341:Aromatherapy; D049930:Chrysopogon / Q000737:chemistry*; D003975:Diazepam; D008297:Male; D009822:Oils, Volatile / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D017208:Rats, Wistar; D013315:Stress, Psychological
chemical_list: D014151:Anti-Anxiety Agents; D009822:Oils, Volatile; D016760:Proto-Oncogene Proteins c-fos; D003975:Diazepam
keywords: amygdala; anxiety; diazepam; elevated plus-maze; vetiver essential oils