title: Hyaluronic acid prevents immunosuppressive drug-induced ovarian damage via up-regulating PGRMC1 expression.
abstract: Chemotherapy treatment in women can frequently cause damage to the ovaries, which may lead to primary ovarian insufficiency (POI). In this study, we assessed the preventative effects of hyaluronic acid (HA) in immunosuppressive drug-induced POI-like rat models and investigated the possible mechanisms. We found that HA, which was reduced in primary and immunosuppressant-induced POI patients, could protect the immunosuppressant-induced damage to granulosa cells (GCs) in vitro. Then we found that HA blocked the tripterygium glycosides (TG) induced POI-like presentations in rats, including delayed or irregular estrous cycles, reduced 17 beta-estradiol(E2) concentration, decreased number of follicles, destruction of follicle structure, and damage of reproductive ability. Furthermore, we investigated the mechanisms of HA prevention effects on POI, which was associated with promotion of GC proliferation and PGRMC1 expression. In conclusion, HA prevents chemotherapy-induced ovarian damage by promoting PGRMC1 in GCs. This study may provide a new strategy for prevention and treatment of POI.
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Yan
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School &State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fang
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Haixiang
authors.initials: H
authors.lastname: Sun
authors.affiliation: Immunology and Reproductive Biology Laboratory, Medical School &State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China.
authors.forename: Yayi
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing 210008, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000328:Adult; D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004958:Estradiol / Q000378:metabolism; D005260:Female; D006027:Glycosides / Q000633:toxicity; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D007166:Immunosuppressive Agents / Q000627:therapeutic use / Q000633:toxicity*; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009369:Neoplasms / Q000188:drug therapy; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism; D016649:Primary Ovarian Insufficiency / Q000139:chemically induced / Q000517:prevention & control*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D011980:Receptors, Progesterone / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012098:Reproduction / Q000187:drug effects; D029757:Tripterygium / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*; D055815:Young Adult
chemical_list: D006027:Glycosides; D007166:Immunosuppressive Agents; D008565:Membrane Proteins; C486424:Pgrmc1 protein, rat; D034741:RNA, Small Interfering; D011980:Receptors, Progesterone; D004958:Estradiol; D006820:Hyaluronic Acid; D001141:Aromatase