title: Genetic characterisation of drug resistance and clonal dynamics of Acinetobacter baumannii in a hospital setting in Mexico.
abstract: The aim of this study was to determine the clinical characteristics, molecular epidemiology and biofilm production of Acinetobacter baumannii clinical isolates obtained from a tertiary-care hospital in Mexico. Clinical isolates of A. baumannii (n=152) isolated from 2007 to 2012 were included. Clonal diversity was analysed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Antimicrobial susceptibility was determined using the broth microdilution method. IMP, VIM, NDM and OXA-type genes were screened by PCR. Biofilm production was analysed using the crystal violet method. Mortality attributable to A. baumannii infection was 14.5%. Fifty-four clones were detected, of which five predominated. MLST results showed three new sequence types and two reported sequence types. More than 86% of the isolates were resistant to ciprofloxacin, ceftazidime and cefotaxime. Furthermore, 50.7% and 35.5% of the isolates were resistant to imipenem and meropenem, respectively. Of the isolates evaluated, 28.3% and 25.7% were positive for the blaOXA-58 and blaOXA-72 genes, respectively. Biofilm production was associated with resistance to imipenem (P=0.002).
authors.affiliation: Servicio de Gastroenterología, Hospital Universitario Dr José Eleuterio González, Nuevo León, Mexico.
authors.forename: Paola
authors.initials: P
authors.lastname: Bocanegra-Ibarias
authors.affiliation: Departamento de Medicina Interna, Hospital Universitario Dr José Eleuterio González, Nuevo León, Mexico.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Peña-López
authors.affiliation: Servicio de Infectología, Coordinación de Epidemiología Hospitalaria, Hospital Universitario Dr José Eleuterio González, Nuevo León, Mexico.
authors.forename: Adrian
authors.initials: A
authors.lastname: Camacho-Ortiz
authors.affiliation: Departamento de Patología Clínica, Hospital Universitario Dr José Eleuterio González, Universidad Autónoma de Nuevo León, Nuevo León, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Llaca-Díaz
authors.affiliation: Instituto Nacional de Salud Pública, Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico.
authors.forename: Jesús
authors.initials: J
authors.lastname: Silva-Sánchez
authors.affiliation: Instituto Nacional de Salud Pública, Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico.
authors.forename: Humberto
authors.initials: H
authors.lastname: Barrios
authors.affiliation: Instituto Nacional de Salud Pública, Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico.
authors.forename: Ulises
authors.initials: U
authors.lastname: Garza-Ramos
authors.affiliation: Departamento de Medicina Interna, Hospital Universitario Dr José Eleuterio González, Nuevo León, Mexico.
authors.forename: Adrian Marcelo
authors.initials: AM
authors.lastname: Rodríguez-Flores
authors.affiliation: Servicio de Gastroenterología, Hospital Universitario Dr José Eleuterio González, Nuevo León, Mexico; Departamento de Patología Clínica, Hospital Universitario Dr José Eleuterio González, Universidad Autónoma de Nuevo León, Nuevo León, Mexico. Electronic address: elvira_garza_gzz@yahoo.com.
authors.forename: Elvira
authors.initials: E
authors.lastname: Garza-González
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology* / Q000401:mortality; D040981:Acinetobacter baumannii / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000401:mortality; D024881:Drug Resistance, Bacterial; D005260:Female; D005838:Genotype; D006761:Hospitals; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D016019:Survival Analysis; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases
keywords: Acinetobacter; Biofilm; Carbapenemases; OXA-58; OXA-72; PFGE