title: Antioxidant and DNA damage protection potentials of selected phenolic acids.
abstract: In this study, ten different phenolic acids (caffeic, chlorogenic, cinnamic, ferulic, gallic, p-hydroxybenzoic, protocatechuic, rosmarinic, syringic, and vanillic acids) were evaluated for their antioxidant and DNA damage protection potentials. Antioxidant activity was evaluated by using four different test systems named as β-carotene bleaching, DPPH free radical scavenging, reducing power and chelating effect. In all test systems, rosmarinic acid showed the maximum activity potential, while protocatechuic acid was determined as the weakest antioxidant in β-carotene bleaching, DPPH free radical scavenging, and chelating effect assays. Phenolic acids were also screened for their protective effects on pBR322 plasmid DNA against the mutagenic and toxic effects of UV and H2O2. Ferulic acid was found as the most active phytochemical among the others. Even at the lowest concentration value (0.002 mg/ml), ferulic acid protected all of the bands in the presence of H2O2 and UV. It is followed by caffeic, rosmarinic, and vanillic acids. On the other hand, cinnamic acid (at 0.002 mg/ml), gallic acid (at 0.002 mg/ml), p-hydroxybenzoic acid (at 0.002 and 0.004 mg/ml), and protocatechuic acid (at 0.002 and 0.004 mg/ml) could not protect plasmid DNA.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Literature, Kilis 7 Aralık University, TR-79000 Kilis, Turkey.
authors.forename: Kemal
authors.initials: K
authors.lastname: Sevgi
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science and Literature, Kilis 7 Aralık University, TR-79000 Kilis, Turkey. Electronic address: bektastepe@yahoo.com.
authors.forename: Bektas
authors.initials: B
authors.lastname: Tepe
authors.affiliation: Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Suleyman Demirel University, TR-32000 Isparta, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Sarikurkcu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002934:Cinnamates / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects*; D053630:Depsides / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000633:toxicity; D062385:Hydroxybenzoates / Q000737:chemistry*; D019787:Linoleic Acid / Q000737:chemistry; D064209:Phytochemicals / Q000737:chemistry; D014466:Ultraviolet Rays / Q000009:adverse effects; D014641:Vanillic Acid / Q000737:chemistry; D019207:beta Carotene / Q000737:chemistry; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D002934:Cinnamates; D003373:Coumaric Acids; D053630:Depsides; D005707:Gallic Acid; D006861:Hydrogen Peroxide; D062385:Hydroxybenzoates; D019787:Linoleic Acid; D064209:Phytochemicals; D014641:Vanillic Acid; D019207:beta Carotene; D000097123:Rosmarinic Acid; C029010:cinnamic acid; C009091:protocatechuic acid; C004999:ferulic acid; C017616:phenolic acid
keywords: Antioxidant activity; DNA damage protection potential; Ferulic acid; Rosmarinic acid; pBR322 plasmid DNA