title: Protein hairy enhancer of split-1 expression during differentiation of muscle-derived stem cells into neuron-like cells.
abstract: Muscle-derived stem cells were isolated from the skeletal muscle of Sprague-Dawley neonatal rats aged 3 days old. Cells at passage 5 were incubated in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) fetal bovine serum, 20 μg/L nerve growth factor, 20 μg/L basic fibroblast growth factor and 1% (v/v) penicillin for 6 days. Cells presented with long processes, similar to nerve cells. Connections were formed between cell processes. Immunocytochemical staining with neuron specific enolase verified that cells differentiated into neuron-like cells. Immunofluorescence cytochemistry and western blot results revealed that the expression of protein hairy enhancer of split-1 was significantly reduced. These results indicate that low expression of protein hairy enhancer of split-1 participates in the differentiation of muscle-derived stem cells into neuron-like cells.
authors.affiliation: Department of Nursing College, Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Mina
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Zhanpeng
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Nursing College, Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Xifan
authors.initials: X
authors.lastname: Mei
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Fang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Jinhao
authors.initials: J
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, First Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China.
authors.forename: Yajiang
authors.initials: Y
authors.lastname: Yuan
keywords: muscle-derived stem cells; neural regeneration; neuron specific enolase; neuron-like cells; proliferation; protein hairy enhancer of split-1