title: Rapid DNA extraction from dried blood spots on filter paper: potential applications in biobanking.
abstract: OBJECTIVE
  Dried blood spot (DBS) technology is a microsampling alternative to traditional plasma or serum sampling for pharmaco- or toxicokinetic evaluation. DBS technology has been applied to diagnostic screening in drug discovery, nonclinical, and clinical settings. We have developed an improved elution protocol involving boiling of blood spots dried on Whatman filter paper.
  
  
  METHODS
  The purpose of this study was to compare the quality, purity, and quantity of DNA isolated from frozen blood samples and DBSs. We optimized a method for extraction and estimation of DNA from blood spots dried on filter paper (3-mm FTA card). A single DBS containing 40 μL blood was used.
  
  
  RESULTS
  DNA was efficiently extracted in phosphate-buffered saline (PBS) or Tris-EDTA (TE) buffer by incubation at 37°C overnight. DNA was stable in DBSs that were stored at room temperature or frozen. The housekeeping genes GAPDH and beta-actin were used as positive standards for polymerase chain reaction (PCR) validation of general diagnostic screening.
  
  
  CONCLUSIONS
  Our simple and convenient DBS storage and extraction methods are suitable for diagnostic screening by using very small volumes of blood collected on filter paper, and can be used in biobanks for blood sample storage.
authors.affiliation: Eulji Medi-Bio Research Institute (EMBRI), Daejeon, Korea.
authors.forename: Eun-Hye
authors.initials: EH
authors.lastname: Choi
authors.affiliation: Eulji Medi-Bio Research Institute (EMBRI), Daejeon, Korea.
authors.forename: Sang Kwang
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Eulji Medi-Bio Research Institute (EMBRI), Daejeon, Korea ; Department of Laboratory Medicine, Eulji University Hospital, Daejeon, Korea.
authors.forename: Chunhwa
authors.initials: C
authors.lastname: Ihm
authors.affiliation: Department of Laboratory Medicine, Eulji University Hospital, Daejeon, Korea.
authors.forename: Young-Hak
authors.initials: YH
authors.lastname: Sohn
keywords: biobank; dried blood spot; filter paper