title: Challenges of analyzing different classes of metabolites by a single analytical method.
abstract: Complex biological samples include thousands of metabolites that range widely in both physiochemical properties and concentration. Simultaneously analyzing metabolites with different properties using a single analytical method is very challenging. The analytical process for metabolites comprises multiple steps including sampling, quenching, sample preparation, separation and detection. Each step can have a significant effect on the reliability and precision of ultimate analytic results. The aim of review is a discussion of considerations and challenges for the simultaneous analysis of metabolites using LC- and GC-MS systems. The review discusses available methodology for each analytical step, and presents the limitations and advantages of each method for the large-scale targeted metabolomics analysis of human and animal biological samples.
authors.affiliation: Terrence Donnelly Centre for Cellular & Biomolecular Research, University of Toronto, ON, M5S 3E1, Canada.
authors.forename: Fatemeh S
authors.initials: FS
authors.lastname: Mirnaghi
authors.forename: Amy A
authors.initials: AA
authors.lastname: Caudy
mesh_terms: D053000:Analytic Sample Preparation Methods; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D006801:Humans; D013058:Mass Spectrometry; D055432:Metabolomics / Q000379:methods*; D015203:Reproducibility of Results