title: Dictyostelium discoideum Ax2 as an Assay System for Screening of Pharmacological Chaperones for Phenylketonuria Mutations.
abstract: In this study, we developed an assay system for missense mutations in human phenylalanine hydroxylases (hPAHs). To demonstrate the reliability of the system, eight mutant proteins (F39L, K42I, L48S, I65T, R252Q, L255V, S349L, and R408W) were expressed in a mutant strain (pah(-)) of Dictyostelium discoideum Ax2 disrupted in the indigenous gene encoding PAH. The transformed pah- cells grown in FM minimal medium were measured for growth rate and PAH activity to reveal a positive correlation between them. The protein level of hPAH was also determined by western blotting to show the impact of each mutation on protein stability and catalytic activity. The result was highly compatible with the previous ones obtained from other expression systems, suggesting that Dictyostelium is a dependable alternative to other expression systems. Furthermore, we found that both the protein level and activity of S349L and R408W, which were impaired severely in protein stability, were rescued in HL5 nutrient medium. Although the responsible component(s) remains unidentified, this unexpected finding showed an important advantage of our expression system for studying unstable proteins. As an economic and stable cell-based expression system, our development will contribute to mass-screening of pharmacological chaperones for missense PAH mutations as well as to the in-depth characterization of individual mutations.
authors.affiliation: School of Biological Sciences, Inje University, Kimhae 621-749, Republic of Korea.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Inje University, Kimhae 621-749, Republic of Korea.
authors.forename: Yun Gyeong
authors.initials: YG
authors.lastname: Yang
authors.affiliation: School of Biological Sciences, Inje University, Kimhae 621-749, Republic of Korea.
authors.forename: Hye-Lim
authors.initials: HL
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Inje University, Kimhae 621-749, Republic of Korea.
authors.forename: Young Shik
authors.initials: YS
authors.lastname: Park
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D004023:Dictyostelium / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004795:Enzyme Stability / Q000187:drug effects*; D057166:High-Throughput Screening Assays; D006801:Humans; D050505:Mutant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010651:Phenylalanine Hydroxylase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D003470:Culture Media; D050505:Mutant Proteins; D011994:Recombinant Proteins; D010651:Phenylalanine Hydroxylase