title: Human olfactory bulb neural stem cells mitigate movement disorders in a rat model of Parkinson's disease.
abstract: Parkinson's disease (PD) is a neurological disorder characterized by the loss of midbrain dopaminergic (DA) neurons. Neural stem cells (NSCs) are multipotent stem cells that are capable of differentiating into different neuronal and glial elements. The production of DA neurons from NSCs could potentially alleviate behavioral deficits in Parkinsonian patients; timely intervention with NSCs might provide a therapeutic strategy for PD. We have isolated and generated highly enriched cultures of neural stem/progenitor cells from the human olfactory bulb (OB). If NSCs can be obtained from OB, it would alleviate ethical concerns associated with the use of embryonic tissue, and provide an easily accessible cell source that would preclude the need for invasive brain surgery. Following isolation and culture, olfactory bulb neural stem cells (OBNSCs) were genetically engineered to express hNGF and GFP. The hNFG-GFP-OBNSCs were transplanted into the striatum of 6-hydroxydopamin (6-OHDA) Parkinsonian rats. The grafted cells survived in the lesion environment for more than eight weeks after implantation with no tumor formation. The grafted cells differentiated in vivo into oligodendrocyte-like (25 ± 2.88%), neuron-like (52.63 ± 4.16%), and astrocyte -like (22.36 ± 1.56%) lineages, which we differentiated based on morphological and immunohistochemical criteria. Transplanted rats exhibited a significant partial correction in stepping and placing in non-pharmacological behavioral tests, pole and rotarod tests. Taken together, our data encourage further investigations of the possible use of OBNSCs as a promising cell-based therapeutic strategy for Parkinson's disease.
authors.affiliation: Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Hany E S
authors.initials: HE
authors.lastname: Marei
authors.forename: Samah
authors.initials: S
authors.lastname: Lashen
authors.forename: Amany
authors.initials: A
authors.lastname: Farag
authors.forename: Asmaa
authors.initials: A
authors.lastname: Althani
authors.forename: Nahla
authors.initials: N
authors.lastname: Afifi
authors.forename: Abd-Elmaksoud
authors.initials: AE
authors.lastname: A
authors.forename: Shaymaa
authors.initials: S
authors.lastname: Rezk
authors.forename: Roberto
authors.initials: R
authors.lastname: Pallini
authors.forename: Patrizia
authors.initials: P
authors.lastname: Casalbore
authors.forename: Carlo
authors.initials: C
authors.lastname: Cenciarelli
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D008297:Male; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D058953:Neural Stem Cells / Q000637:transplantation*; D009830:Olfactory Bulb / Q000166:cytology*; D016627:Oxidopamine / Q000633:toxicity; D010300:Parkinson Disease / Q000209:etiology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D033581:Stem Cell Transplantation / Q000379:methods*
chemical_list: D016627:Oxidopamine; D020932:Nerve Growth Factor