title: [Effect of honokiol on proliferation and apoptosis in HL-60 cells and its potential mechanism].
abstract: This study was aimed to investigate the effect of Honokiol (HNK) on proliferation and apoptosis of acute myeloid leukemia HL-60 cells and its potential mechanism. Inhibitory effect of HNK on the HL-60 cell proliferation was detected by MTT assay. Flow cytometry was used to detect the change of cell cycle and AnnexinV/PI staining was used to detect apoptosis. Western blot was applied to analyze the cell cycle protein (cyclins), cyclin-dependent kinase (CDK), P53, P21, P27, BCL-2, BCL-XL, Bax, caspase-3/9 and proteins for MAPK signal pathway. The results showed that HNK could inhibit the proliferation of HL-60 cells in time- and dose dependent ways. HNK arrested HL-60 cells in G0/G1 phase, and S phase cells decreased significantly (P < 0.05). The expression of cyclin D1, cyclin A, cyclin E and CDK2/4/6 were significantly down-regulated (P < 0.05), the expression of P53 and P21 was significantly upregulated after treating for 24 h with HNK (P < 0.05). After 24 h treatment with HNK, HL-60 cell apoptosis increased significantly with the upregulation of activated caspase-3, -9, BAX expression and the downregulation of BCL-2, BCL-XL expression. The MAPK subfamily, P38 and JNK were not significantly changed, but the expression of MEK1/2-ERK1/2 was significantly downregulated (P < 0.05). It is concluded that HNK arrestes the cells at G0/G1 phase and induces HL-60 cell apoptosis through the intervention of MEK1/2-ERK1/2 signaling pathway.
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Fan
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China. E-mail: Zengyingjian1975@163.com.
authors.forename: Ying-Jian
authors.initials: YJ
authors.lastname: Zeng
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Guang-Yang
authors.initials: GY
authors.lastname: Weng
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wu
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China.
authors.forename: Zhang-Qiu
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Li
authors.affiliation: Department of Hematology, Wuyi Hospital of Traditional Chinese Medicine/Traditional Chinese Medicine Hospital of Jinan University Medical College, Jiangmen 529000, Guangdong Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Department of Hematology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.
authors.forename: Kun-Yuan
authors.initials: KY
authors.lastname: Guo
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D053148:Caspase 3; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1; D019927:Cyclin E; D051357:Cyclin-Dependent Kinase 2; D018922:HL-60 Cells; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D015513:Oncogene Proteins; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D001713:Biphenyl Compounds; D053148:Caspase 3; D019938:Cyclin D1; D019927:Cyclin E; D051357:Cyclin-Dependent Kinase 2; D017705:Lignans; D015513:Oncogene Proteins; D051028:bcl-2-Associated X Protein; C475820:CCNE1 protein, human; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols; C495894:CDK2 protein, human