title: Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase.
abstract: Mutants of toluene o-xylene monooxygenase are demonstrated to oxidize ethylene to ethylene oxide in vivo at yields of >99%. The best mutant increases ethylene oxidation activity by >5500-fold relative to the native enzyme. This is the first report of a recombinant enzyme capable of carrying out this industrially significant chemical conversion.
authors.affiliation: Biophysics Graduate Group, University of California Davis, Davis, California, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Carlin
authors.forename: S J
authors.initials: SJ
authors.lastname: Bertolani
authors.forename: J B
authors.initials: JB
authors.lastname: Siegel
mesh_terms: D001665:Binding Sites; D055162:Biocatalysis; D005027:Ethylene Oxide / Q000737:chemistry / Q000378:metabolism*; D005030:Ethylenes / Q000737:chemistry / Q000378:metabolism*; D056004:Molecular Dynamics Simulation; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D005030:Ethylenes; D011994:Recombinant Proteins; C036216:ethylene; D010105:Oxygenases; C114360:toluene 2-xylene monooxygenase; D005027:Ethylene Oxide