title: Amelioration of lipid peroxidation in vivo and in vitro by Satureja khozestanica essential oil in alloxan-induced diabetic rats.
abstract: BACKGROUND
  We examined possible protective effect of Satureja khozestanica essential oil (SKE) on in vivo and in vitro lipid peroxidation in alloxan-induced Type 1 diabetic rats.
  
  
  METHODS
  Thirty Sprage-dawley male rats were divided into three groups randomly; group one as control, group two diabetic untreatment, and group three treatments with SKE by 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, livers and kidneys were then removed immediately and used fresh or kept frozen until their lipid peroxidation analysis. Lipid peroxidation was determined by measurement of thiobarbituric acid reactive substances (TBARS). Blood samples were also collected before killing to measure the levels of fasting blood suger (FBS) and lipid peroxidation.
  
  
  RESULTS
  SKE significantly inhibited the levels of FBS, TBARS serum and kidney content in treated group compared with the diabetic untreated group. Also the levels of malonedialdehyde liver content unaltered in treated group. SKE significantly inhibited LDL oxidation in vitro.
  
  
  CONCLUSIONS
  The findings showed that SKE exerts beneficial effects on the lipid peroxidation in alloxan-induced Type 1 diabetic rats.
authors.affiliation: Razi Herbal Researches Center, Lorestan University of Medical Sciences, Khoram Abad, Iran ; Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Ahmadvand
authors.affiliation: Department of Anatomy, Faculty, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Tavafi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khosrowbeygi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Shahsavari
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Hormozi
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Beyranvand
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Bagheri
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khoram Abad, Iran.
authors.forename: Foad
authors.initials: F
authors.lastname: Abdolahpour
keywords: Diabetes; Essential oil; Lipid peroxidation; Rat; Satureja khozestanica