title: Near-infrared reflectance spectroscopy (NIRS) for rapid determination of ginsenoside Rg1 and Re in Chinese patent medicine Naosaitong pill.
abstract: Ginsenosides in plant samples have been extensively studied because protopanaxadiol saponins are ubiquitous in Chinese patent medicines, in which they can be used in promoting human health as the main active ingredients. A method for rapid determination of two ginsenosides (Rg1 and Re) in Naosaitong (NST) samples using near-infrared reflectance spectroscopy (NIRS) is studied to determine the contents of ginsenoside Rg1 and Re in this work. Partial least square (PLS) regression was used for building the calibration models, and the effects of spectral preprocessing and variable selection on the models are investigated for optimization of the models. A total of 93 samples were scanned by NIRS, and also by high performance liquid chromatography coupled to a diode array detector to determine the contents of ginsenoside Rg1 and Re. The calibration models for Rg1 and Re had high values of the coefficient of determination (R(2)) (0.9766 and 0.9764) and low root mean square error of cross validation (RMSECV) (0.0136 and 0.0104), and the values of the standard error of prediction set (SEP) are 0.00764 and 0.0103, which indicate a good correlation between reference values and NIRS predicted values. The overall results show that NIRS could be applied for the rapid determination of the contents of ginsenosides in Ginseng byproducts for pharmaceuticals that develop high-quality Chinese patent medicines.
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, PR China.
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Qu
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, PR China. Electronic address: yingpingwcaas@126.com.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, PR China.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Yao
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, PR China. Electronic address: baixy1212@163.com.
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Bai
authors.affiliation: Changchun University of Chinese Medicine, Changchun 130117, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Bian
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides / Q000032:analysis*; D016018:Least-Squares Analysis; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013607:Tablets
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D013607:Tablets; C035054:ginsenoside Rg1
keywords: Naosaitong pill; Near-infrared spectroscopy; Partial least square