title: Oestrogen receptor alpha expression in neovaginal tissue of women following modified Abbé-McIndoe technique and in premenopausal women.
abstract: The aim of the study was to compare the expression of oestrogen receptor alpha (ERα) in neovaginal tissue of patients with vaginal agenesis following neovaginoplasty using regenerated, oxidised cellulose in premenopausal women. A prospective, observational case-control study was performed on eight patients with vaginal agenesis following modified Abbé-McIndoe neovaginoplasty and 10 control premenopausal women following benign gynaecologic surgery. 6F11 monoclonal antibody was used to determine ERα expression in the vaginal mucosa. Quantitative and qualitative evaluations were performed, respectively, in vaginal epithelium and stroma. The thickness of the vaginal epithelium was determined as the vertical distance between the basal layer cells and the apical surface of the superficial layer. The percentage of ERα-expressing cells was higher in the control group, except in the superficial zone of the epithelium. In the stromal tissue, ERα was detected in only one patient from the neovagina group compared with nine women in the control group. The neovagina group had a statistically thinner epithelium. Our study suggests that women with vaginal agenesis following modified Abbé-McIndoe neovaginoplasty using regenerated oxidised, cellulose experience relatively local hypo-oestrogenism in the first year after surgery, with repercussion in vaginal trophism.
authors.affiliation: Department of Gynaecology at Paulista School of Medicine - São Paulo Federal University (EPM-UNIFESP) , São Paulo-SP , Brazil .
authors.forename: Marcio Masashi
authors.initials: MM
authors.lastname: Kajikawa
authors.forename: Zsuzsanna Ilona Katalin
authors.initials: ZI
authors.lastname: Jármy-Di Bella
authors.forename: Juliane
authors.initials: J
authors.lastname: Dornelas
authors.forename: Luciana Campanatti
authors.initials: LC
authors.lastname: Crema
authors.forename: Cláudia Cristina
authors.initials: CC
authors.lastname: Takano
authors.forename: Gustavo Rubino de Azevedo
authors.initials: GR
authors.lastname: Focchi
authors.forename: Liliam Cristine
authors.initials: LC
authors.lastname: Rolo
authors.forename: Edward
authors.initials: E
authors.lastname: Araujo Júnior
authors.forename: Rodrigo Aquino
authors.initials: RA
authors.lastname: Castro
authors.forename: Manoel João Batista Castello
authors.initials: MJ
authors.lastname: Girão
authors.forename: Marair Gracio Ferreira
authors.initials: MG
authors.lastname: Sartori
mesh_terms: D058489:46, XX Disorders of Sex Development / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D000293:Adolescent; D000328:Adult; D001284:Atrophy; D001706:Biopsy; D001938:Brazil; D016022:Case-Control Studies; D002483:Cellulose, Oxidized / Q000627:therapeutic use; D000013:Congenital Abnormalities / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D047628:Estrogen Receptor alpha / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D048091:Guided Tissue Regeneration / Q000009:adverse effects; D013509:Gynecologic Surgical Procedures / Q000009:adverse effects; D006785:Hospitals, University; D006801:Humans; D009092:Mucous Membrane / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D009095:Mullerian Ducts / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D017697:Premenopause; D011446:Prospective Studies; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D054457:Tissue Scaffolds; D014621:Vagina / Q000002:abnormalities / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D055815:Young Adult
chemical_list: D002483:Cellulose, Oxidized; C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha
keywords: Abbé-McIndoe technique; neovagina; oestrogen receptor; premenopausal women