title: Cyclodextrin-complexed Ocimum basilicum leaves essential oil increases Fos protein expression in the central nervous system and produce an antihyperalgesic effect in animal models for fibromyalgia.
abstract: O. basilicum leaves produce essential oils (LEO) rich in monoterpenes. The short half-life and water insolubility are limitations for LEO medical uses. β-Cyclodextrin (β-CD) has been employed to improve the pharmacological properties of LEO. We assessed the antihyperalgesic profile of LEO, isolated or complexed in β-CD (LEO/β-CD), on an animal model for fibromyalgia. Behavioral tests: mice were treated every day with either LEO/β-CD (25, 50 or 100 mg/kg, p.o.), LEO (25 mg/kg, p.o.), tramadol (TRM 4 mg/kg, i.p.) or vehicle (saline), and 60 min after treatment behavioral parameters were assessed. Therefore, mice were evaluated for mechanical hyperalgesia (von Frey), motor coordination (Rota-rod) and muscle strength (Grip Strength Metter) in a mice fibromyalgia model. After 27 days, we evaluated the central nervous system (CNS) pathways involved in the effect induced by experimental drugs through immunofluorescence protocol to Fos protein. The differential scanning analysis (DSC), thermogravimetry/derivate thermogravimetry (TG/DTG) and infrared absorption spectroscopy (FTIR) curves indicated that the products prepared were able to incorporate the LEO efficiently. Oral treatment with LEO or LEO-βCD, at all doses tested, produced a significant reduction of mechanical hyperalgesia and we were able to significantly increase Fos protein expression. Together, our results provide evidence that LEO, isolated or complexed with β-CD, produces analgesic effects on chronic non-inflammatory pain as fibromyalgia.
authors.affiliation: Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil. simonenascimento.saude@gmail.com.
authors.forename: Simone S
authors.initials: SS
authors.lastname: Nascimento
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. adriasa2001@yahoo.com.br.
authors.forename: Adriano A S
authors.initials: AA
authors.lastname: Araújo
authors.affiliation: Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil. guedes_renan@hotmail.com.
authors.forename: Renan G
authors.initials: RG
authors.lastname: Brito
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. maiserafini@hotmail.com.
authors.forename: Mairim R
authors.initials: MR
authors.lastname: Serafini
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. paulamenezes_16@yahoo.com.br.
authors.forename: Paula P
authors.initials: PP
authors.lastname: Menezes
authors.affiliation: Department of Physical Therapy, Federal University of Sergipe, Aracaju, Sergipe 49.060-108, Brazil. josimelo@infonet.com.br.
authors.forename: Josimari M
authors.initials: JM
authors.lastname: DeSantana
authors.affiliation: Department of Morphology, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. wlucca1@gmail.com.
authors.forename: Waldecy
authors.initials: W
authors.lastname: Lucca
authors.affiliation: Department of Chemistry, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. periclesbalves@gmail.com.
authors.forename: Pericles B
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Department of Agronomic Engineering, Federal University of Sergipe, São Cristóvão, Sergipe 49.100-000, Brazil. afblank@ufs.br.
authors.forename: Arie F
authors.initials: AF
authors.lastname: Blank
authors.affiliation: Medicinal Plants Research Center, Federal University of Piauí, Teresina, Piauí 64.049-550, Brazil. menesesoliveira@gmail.com.
authors.forename: Rita C M
authors.initials: RC
authors.lastname: Oliveira
authors.affiliation: Medicinal Plants Research Center, Federal University of Piauí, Teresina, Piauí 64.049-550, Brazil. aldeidia@gmail.com.
authors.forename: Aldeidia P
authors.initials: AP
authors.lastname: Oliveira
authors.affiliation: Institute of Technology and Research, University Tiradentes, Aracaju, Sergipe 49.032-490, Brazil. ricardo_luiz@itp.org.br.
authors.forename: Ricardo L C
authors.initials: RL
authors.lastname: Albuquerque
authors.affiliation: Department of Pharmacy, Federal University of San Francisco Valley, Petrolina, Pernambuco 56.304-917, Brazil. guedesjackson@yahoo.com.br.
authors.forename: Jackson R G S
authors.initials: JR
authors.lastname: Almeida
authors.affiliation: Laboratory of Pre-Clinical Pharmacology (LAPEC), Department of Physiology, Federal University of Sergipe, Av. Tancredo Neves, S/N, Rosa Elza, CEP: 49.000-100, São Cristóvão, Sergipe 49.100-000, Brazil. lucindojr@gmail.com.
authors.forename: Lucindo J
authors.initials: LJ
authors.lastname: Quintans
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D005356:Fibromyalgia / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D018737:Hand Strength; D006930:Hyperalgesia / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D008297:Male; D051379:Mice; D039821:Monoterpenes / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D009043:Motor Activity / Q000187:drug effects; D018649:Ocimum basilicum / Q000737:chemistry*; D009822:Oils, Volatile / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics*; D015854:Up-Regulation / Q000187:drug effects; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000700:Analgesics; D039821:Monoterpenes; D009822:Oils, Volatile; D016760:Proto-Oncogene Proteins c-fos; D047392:beta-Cyclodextrins; C031215:betadex