title: Phosphorylation of Heat Shock Protein 27 is Increased by Cast Immobilization and by Serum-free Starvation in Skeletal Muscles.
abstract: [Purpose] Cast immobilization- and cell starvation-induced loss of muscle mass are closely associated with a dramatic reduction in the structural muscle proteins. Heat shock proteins are molecular chaperones that are constitutively expressed in several eukaryotic cells and have been shown to protect against various stressors. However, the changes in the phosphorylation of atrophy-related heat shock protein 27 (HSP27) are still poorly understood in skeletal muscles. In this study, we examine whether or not phosphorylation of HSP27 is changed in the skeletal muscles after cast immobilization and serum-free starvation with low glucose in a time-dependent manner. [Methods] We undertook a HSP27 expression and high-resolution differential proteomic analysis in skeletal muscles. Furthermore, we used western blotting to examine protein expression and phosphorylation of HSP27 in atrophied gastrocnemius muscle strips and L6 myoblasts. [Results] Cast immobilization and starvation significantly upregulated the phosphorylation of HSP27 in a time-dependent manner, respectively. [Conclusion] Our results suggest that cast immobilization- and serum-free starvation-induced atrophy may be in part related to changes in the phosphorylation of HSP27 in rat skeletal muscles.
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Mee-Young
authors.initials: MY
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Jeong-Uk
authors.initials: JU
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Lim-Kyu
authors.initials: LK
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Byoung-Sun
authors.initials: BS
authors.lastname: Park
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Seung-Min
authors.initials: SM
authors.lastname: Yang
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Hye-Joo
authors.initials: HJ
authors.lastname: Jeon
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Won-Deok
authors.initials: WD
authors.lastname: Lee
authors.affiliation: Laboratory of Health Science and Nanophysiotherapy, Department of Physical Therapy, Graduate School, Yongin University, Republic of Korea.
authors.forename: Ji-Woong
authors.initials: JW
authors.lastname: Noh
authors.affiliation: Department of Taekwondo Instructor Education, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Taek-Yong
authors.initials: TY
authors.lastname: Kwak
authors.affiliation: Department of Judo, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Jang
authors.affiliation: Department of Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Tae-Hyun
authors.initials: TH
authors.lastname: Lee
authors.affiliation: Department of Combative Martial Arts Training, College of Martial Arts, Yongin University, Republic of Korea.
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Institute of Functional Genomics, Department of Physiology, School of Medicine, Konkuk University, Republic of Korea.
authors.forename: Bokyung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Physical Therapy, College of Public Health and Welfare, Yongin University: Yongin 449-714, Republic of Korea.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Kim
keywords: Cast immobilization and serum-free starvation; Heat shock protein 27; Muscle atrophy