title: Changes on digestive enzymes during initial ontogeny in the three-spot cichlid Cichlasoma trimaculatum.
abstract: A study was performed in order to understand the development of digestive enzymes during initial ontogeny of Cichlasoma trimaculatum, for which the activity of acidic and alkaline proteases, lipases, amylases and phosphatases was determined by means of biochemical and electrophoretic analysis. Our results showed that the activity of alkaline proteases, trypsin and chymotrypsin is present from day 6 after hatching (dah) during exogenous feeding with Artemia nauplii. The activities of carboxypeptidase A and leucine aminopeptidase are present from the first days, increasing at 6 dah and reaching their maximum activity at 9 dah while acid protease activity started at 9 dah. Furthermore, the lipase activity is detected on 6 dah and keeps increasing and decreasing on 17 dah. Amylase activity is detected on 3 dah, presenting fluctuations until 45 dah, where it reaches its maximum activity. Acid and alkaline phosphatases are detected from 3 dah and reach a maximum activity between 13 and 19 dah. The SDS-PAGE electrophoresis revealed six types of bands in the alkaline proteases, with molecular weight between 113.4 and 20.4 kDa. First three bands appear on 6 dah, but it is until 11 dah when all isoforms appear. Based on these results, it is considered that this species completes its digestive enzymatic machinery from day 9 after hatching, therefore is recommended to perform the transition from live feed to inert feed at 15 dah.
authors.affiliation: Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, 0.5 km Carretera Villahermosa-Cárdenas, 86039, Villahermosa, TAB, Mexico.
authors.forename: F J
authors.initials: FJ
authors.lastname: Toledo-Solís
authors.forename: A
authors.initials: A
authors.lastname: Uscanga-Martínez
authors.forename: R
authors.initials: R
authors.lastname: Guerrero-Zárate
authors.forename: G
authors.initials: G
authors.lastname: Márquez-Couturier
authors.forename: R
authors.initials: R
authors.lastname: Martínez-García
authors.forename: S
authors.initials: S
authors.lastname: Camarillo-Coop
authors.forename: N
authors.initials: N
authors.lastname: Perales-García
authors.forename: W
authors.initials: W
authors.lastname: Rodríguez-Valencia
authors.forename: M A
authors.initials: MA
authors.lastname: Gómez-Gómez
authors.forename: C A
authors.initials: CA
authors.lastname: Álvarez-González
mesh_terms: D000367:Age Factors; D000681:Amylases / Q000378:metabolism; D000818:Animals; D017756:Aquaculture / Q000379:methods*; D043422:Carboxypeptidases A / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D023681:Cichlids / Q000254:growth & development* / Q000378:metabolism; D004064:Digestive System / Q000201:enzymology* / Q000254:growth & development; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D008049:Lipase; D000681:Amylases; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D043422:Carboxypeptidases A; D002918:Chymotrypsin; D014357:Trypsin