title: Amyloid precursor-like protein 2 C-terminal fragments upregulate S100A9 gene and protein expression in BV2 cells.
abstract: The murine microglial cell line BV2 has neuroprotective effects, but is toxic to neurons by secreting inflammatory cytokines, and is an important target in the treatment of nerve inflammation and neurodegenerative diseases. In the present study, we observed the effects of transfecting three amyloid precursor-like protein 2 (APLP2) C-terminal fragments (CTFs; C57, C50 and C31) in the pEGFP-N1 vector on S100A9 expression in BV2 cells. Reverse transcription-PCR, western blot assay and immunocytochemistry revealed that S100A9 protein and mRNA expression was greater in BV2 cells after CTF transfection than after mock transfection with an empty vector. Furthermore, transfection of full-length APLP2-751 resulted in low levels of S100A9 protein expression. Our results show that APLP2-CTFs upregulate S100A9 protein and mRNA expression in BV2 cells, and identify a novel pathway involved in neuronal injury and apoptosis, and repair and protection in Alzheimer's disease.
authors.affiliation: Department of Psychology, Yanbian Brain Hospital, Yanji, Jilin Province, China.
authors.forename: Guangzhe
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Preventive Medicine, Medical College, Yanbian University, Yanji, Jilin Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Preventive Medicine, Medical College, Yanbian University, Yanji, Jilin Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, China.
authors.forename: Rongjie
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, China.
authors.forename: Junchang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Preventive Medicine, Medical College, Yanbian University, Yanji, Jilin Province, China.
authors.forename: Yanji
authors.initials: Y
authors.lastname: Xu
keywords: APLP2; Alzheimer's disease; BV2 cells; C-terminal fragments; NSFC grant; S100A9; amyloid precursor protein; nerve regeneration; neural regeneration; neurodegeneration; γ-secretase