title: Relationship between certain T helper cytokines and ANA staining: who is the helper?
abstract: OBJECTIVE
  This study investigated some T helper cytokines' association with different ANA positive sera.
  
  
  METHODS
  Sera of 53 ANA-positive subjects (11 were stained of homogeneous pattern, 12 of speckled particle pattern, 9 of nucleolar pattern, 15 of centromere pattern, and 6 of the peripheral nuclear type) were collected and analyzed by ELISA for related cytokines and concentrations were compared with each other and 16 healthy donors.
  
  
  RESULTS
  Investigated cytokines including IL-12(p70) and IFN-γ varied differently in the ANA sera.
  
  
  CONCLUSIONS
  This study demonstrated that some T helper cytokines varied with different ANA-positive sera, suggesting that they may play different roles in related autoimmune diseases.
authors.affiliation: Department of Laboratory Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, China. Electronic address: hjh7677@sina.com.
authors.forename: Jinhui
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Ophthalmology, Huashan Hospital, Fudan University, No. 12, Middle Wulumuqi Road, Shanghai 200040, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, China.
authors.forename: Xinghui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Rheumatology, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wei
authors.affiliation: Department of Laboratory Medicine, Gongli Hospital, Second Military Medicine University, Pudong New Area, Shanghai 200135, China.
authors.forename: Denghai
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000368:Aged; D000974:Antibodies, Antinuclear / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D018664:Interleukin-12 / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013194:Staining and Labeling; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: ANA; Autoimmune; Cytokines; T helper