title: Bioactive DNA-peptide nanotubes enhance the differentiation of neural stem cells into neurons.
abstract: We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson-Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.
authors.affiliation: Simpson Querrey Institute for BioNanotechnology, Feinberg School of Medicine and ‡Department of Neurology, Northwestern University , Chicago, Illinois 60611, United States.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Stephanopoulos
authors.forename: Ronit
authors.initials: R
authors.lastname: Freeman
authors.forename: Hilary A
authors.initials: HA
authors.lastname: North
authors.forename: Shantanu
authors.initials: S
authors.lastname: Sur
authors.forename: Su Ji
authors.initials: SJ
authors.lastname: Jeong
authors.forename: Faifan
authors.initials: F
authors.lastname: Tantakitti
authors.forename: John A
authors.initials: JA
authors.lastname: Kessler
authors.forename: Samuel I
authors.initials: SI
authors.lastname: Stupp
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D004247:DNA / Q000737:chemistry*; D051379:Mice; D045333:Nanotubes, Peptide / Q000737:chemistry*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry*
chemical_list: D045333:Nanotubes, Peptide; D009842:Oligopeptides; D004247:DNA; C046617:arginyl-glycyl-aspartyl-serine
keywords: DNA nanotechnology; DNA-peptide; Self-assembly; biomaterials; extracellular matrix; neural stem cells