title: Effect of artificial musk aqueous extract on the expressions of inflammatory mediators released from lipopolysaccharide-stimulated RAW264.7 cells.
abstract: OBJECTIVE
  To identify the anti-inflammatory effects of artificial musk aqueous extract(AME)on lipopolysaccharide-stimulated cytokines secreted or released by RAW264.7 cells.
  
  
  METHODS
  Cytokines including interleukin(IL)-6,IL-10,and tumor necrosis factor Α were determined using cytokine enzyme-linked immunosorbent assay kits.
  
  
  RESULTS
  Compared with model group,the levels of major cytokines such as tumor necrosis factor Α,IL-6,and IL-10 significantly decreased in different AME groups in a dose-dependent manner.
  
  
  CONCLUSIONS
  In lipopolysaccharide-stimulated RAW264.7 macrophages,AME can remarkably inhibit the release of inflammatory cytokines and thus exerts its anti-inflammatory effects.
authors.affiliation: Department of Technology,Beijing Lianxin Pharmaceutic Co.,Ltd,Beijing 102628,China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Department of Technology,Beijing Lianxin Pharmaceutic Co.,Ltd,Beijing 102628,China.
authors.forename: Qin-Wen
authors.initials: QW
authors.lastname: Zou
authors.affiliation: Beijing Key Laboratory,Institute of Materia Medica,CAMS and PUMC,Beijing 100050,China.
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Bai
authors.affiliation: Beijing Key Laboratory,Institute of Materia Medica,CAMS and PUMC,Beijing 100050,China.
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Cheng
authors.affiliation: Beijing Key Laboratory,Institute of Materia Medica,CAMS and PUMC,Beijing 100050,China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Department of Technology,Beijing Lianxin Pharmaceutic Co.,Ltd,Beijing 102628,China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory,Institute of Materia Medica,CAMS and PUMC,Beijing 100050,China.
authors.forename: Xiu-Yuan
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: Beijing Key Laboratory,Institute of Materia Medica,CAMS and PUMC,Beijing 100050,China.
authors.forename: Xiao-Liang
authors.initials: XL
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005229:Fatty Acids, Monounsaturated; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C008563:musk