title: Simultaneous saccharification and fermentation of delignified lignocellulosic biomass at high solid loadings by a newly isolated thermotolerant Kluyveromyces sp. for ethanol production.
abstract: Simultaneous saccharification and fermentation studies were carried out using thermotolerant newly isolated Kluyveromyces sp. with three different delignified lignocellulosic biomass viz. rice straw, wheat straw and sugarcane bagasse at 5-15% solid loading and 6-12 FPU g(-1) substrate enzyme loading for different time intervals 0-72 h at 42°C. Maximum ethanol achieved from rice straw, wheat straw and sugarcane bagasse with in-house crude cellulases from Aspergillus terreus was 23.23, 18.29 and 17.91 mg mL(-1) at 60 h with 10% solid load and 9 FPU g(-1) substrate enzyme loading. Tween 80 1% (v/v) enhanced the ethanol yield by 8.39%, 9.26% and 8.14% in rice straw, wheat straw and sugarcane bagasse, respectively. External supplementation of β-glucosidase to the crude as well commercial cellulases produced maximum theoretical ethanol yield of 71.76%, 63.77%, 57.15% and 84.56%, 72.47%, 70.55% from rice straw, wheat straw and sugarcane bagasse, respectively.
authors.affiliation: Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120 Gujarat, India. Electronic address: madhuri68@gmail.com.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Narra
authors.affiliation: Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120 Gujarat, India.
authors.forename: Jisha P
authors.initials: JP
authors.lastname: James
authors.affiliation: Sardar Patel Renewable Energy Research Institute, P. Box No. 2, Vallabh Vidyanagar, 388 120 Gujarat, India.
authors.forename: Velmurugan
authors.initials: V
authors.lastname: Balasubramanian
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006601:Hexoses / Q000378:metabolism; D007716:Kluyveromyces / Q000187:drug effects / Q000302:isolation & purification* / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D008969:Molecular Sequence Data; D012275:Oryza / Q000737:chemistry; D010802:Phylogeny; D031786:Saccharum / Q000737:chemistry; D013501:Surface-Active Agents / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000737:chemistry
chemical_list: D006601:Hexoses; D013501:Surface-Active Agents; C036909:lignocellulose; D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase
keywords: Delignification; Ethanol; Lignocellulosic biomass; Simultaneous saccharification and fermentation; Thermotolerant yeast strain