title: DNA binding, photo-induced DNA cleavage and cytotoxicity studies of lomefloxacin and its transition metal complexes.
abstract: This work was focused on a study of the DNA binding and cleavage properties of lomefloxacin (LMF) and its ternary transition metal complexes with glycine. The nature of the binding interactions between compounds and calf thymus DNA (CT-DNA) was studied by electronic absorption spectra, fluorescence spectra and thermal denaturation experiments. The obtained results revealed that LMF and its complexes could interact with CT-DNA via partial/moderate intercalative mode. Furthermore, the DNA cleavage activities of the compounds were investigated by gel electrophoresis. Mechanistic studies of DNA cleavage suggest that singlet oxygen ((1)O2) is likely to be the cleaving agent via an oxidative pathway, except for Cu(II) complex which proceeds via both oxidative and hydrolytic pathways. Antimicrobial and antitumor activities of the compounds were also studied against some kinds of bacteria, fungi and human cell lines.
authors.affiliation: Department of Chemistry, Biochemistry Speciality, Faculty of Science, Cairo University, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Ragheb
authors.affiliation: Department of Chemistry, Biochemistry Speciality, Faculty of Science, Cairo University, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Eldesouki
authors.affiliation: Department of Chemistry, Biochemistry Speciality, Faculty of Science, Cairo University, Egypt. Electronic address: mervat_sayed@yahoo.com.
authors.forename: Mervat S
authors.initials: MS
authors.lastname: Mohamed
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D004583:Electrons; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D024841:Fluoroquinolones / Q000737:chemistry* / Q000633:toxicity; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008027:Light; D008826:Microbial Sensitivity Tests; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D010957:Plasmids / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013696:Temperature; D028561:Transition Elements / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D056831:Coordination Complexes; D024841:Fluoroquinolones; D028561:Transition Elements; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; C053091:lomefloxacin
keywords: Cytotoxic activities; DNA binding and cleavage; Intercalative mode; Lomefloxacin; Transition metal complexes