title: Association between a p73 gene polymorphism and genetic susceptibility to non-small cell lung cancer in the South of China.
abstract: BACKGROUND
  This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China.
  
  
  METHODS
  We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers).
  
  
  RESULTS
  The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/ AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, x2=0.452), tumor stage (p=0.806, x2=0.806), or lymph node metastasis (p=0.578, x2=1.098).
  
  
  CONCLUSIONS
  Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.
authors.affiliation: Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, China E-mail : liwenjoha@yahoo.com.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Wang
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Guo
authors.forename: Lin-Li
authors.initials: LL
authors.lastname: Dong
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Zhu
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Wen
authors.initials: W
authors.lastname: Li
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Tang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins