title: α-Naphthoflavone modulates inflammatory response in adipocytes-macrophages interaction through NFκB signaling.
abstract: OBJECTIVE
  Our previous study demonstrated that α-naphthoflavone (α-NF) inhibits mouse 3T3-L1 pre-adipocytes differentiation via PPARγ, a key transcription factor in adipogenesis. Due to the critical role of inflammation in adipogenesis, we speculated that the suppression role of α-NF in adipogenesis might involve in modulation of cytokines secretion raised by adipocyte differentiation cocktail. Therefore, the present study aims to investigate the role of α-NF in modulating of inflammatory response during adipocytes differentiation and adipocyte-macrophage interaction.
  
  
  METHODS
  Conditioned medium from different doses of α-NF treated 10-day differentiated 3T3-L1 adipocytes were collected to culture RAW264.7 macrophages. Conditioned medium from activated macrophages and α-NF pre-treated macrophage were used to investigate the effects of α-NF in adipocytes differentiation. Cultured cells and medium were harvested for RT-PCR, Western blot and ELISA.
  
  
  RESULTS
  α-NF dose-dependently decreased TNF-α and IL-6 and increased IL-10 expression induced by IDM (Insulin, dexamethasone, isobutylmethylxanthine) in 3T3-L1 pre-adipocytes. Conditioned medium from α-NF treated 3T3-L1 differentiated cells inhibited inflammatory response in mouse macrophage cell line RAW264.7 in contrast to IDM control medium. NFĸB activation elicited by IDM was suppressed by α-NF in a dose-response manner. Consequently, decreased TNF-α and increased IL-10 secretion, downstream targets of NFĸB signaling pathway, were observed with α-NF in macrophages. Finally, Conditioned medium from α-NF pre-treated, LPS-activated macrophages ameliorated the suppression of 3T3-L1 adipogenesis by LPS activated macrophages.
  
  
  CONCLUSIONS
  Our results suggest that α-NF regulates inflammation response in both adipocytes and macrophages and adipocyte-macrophage interaction which contributes to pre-adipocyte differentiation.
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Sicong
authors.initials: S
authors.lastname: Lei
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Fan
authors.affiliation: Department of Urology, Renmin Hospital, Wuhan University China ; Hubei Key Laboratory of Kidney Disease Pathogenesis and Intervention Huangshi, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001571:Benzoflavones / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001571:Benzoflavones; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C011512:alpha-naphthoflavone
keywords: Adipocyte-macrohage interaction; condition medium; inflammation; α-NF