title: Biotransformation of 1,3-propanediol cyclic sulfate and its derivatives to diols by toluene-permeabilized cells of Bacillus sp. CCZU11-1.
abstract: In this study, it was the first report that Bacillus sp. CCZU11-1 was used for the biotransformation of 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives. The catalytic performance of Bacillus sp. sulfatase in the biotransformation of 1,3-PDS was significantly improved by biocatalyst permeabilization and immobilization. Using cell permeabilization, the hydrolytic activity of the whole-cell biocatalyst was increased by 3.5-fold after 1.5 h of pretreatment with 10 % (v/v) toluene at 30 °C and pH 7.0. Biotransformation of 20 mM 1,3-PDS for 24 h, 1,3-propanediol (1,3-PD) could be obtained in the yield of 97.4 % under the optimized reaction condition. Additionally, the immobilized biocatalysts, permeabilized cells entrapped in calcium alginate, and cross-linked enzyme aggregates were further employed to biotansform 1,3-PDS. Moreover, the total operational time of the immobilized biocatalysts could reach above 240 h with high conversion rate (>90 %).
authors.affiliation: Laboratory of Biocatalysis and Bioprocessing, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, 213164, People's Republic of China, yucaihe@cczu.edu.cn.
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Dan-Ping
authors.initials: DP
authors.lastname: Zhang
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Li
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Tao
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
mesh_terms: D001407:Bacillus / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D001711:Biotransformation; D018914:Cells, Immobilized / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D010539:Permeability; D011409:Propylene Glycols / Q000378:metabolism*; D013429:Sulfatases / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D014050:Toluene / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D011409:Propylene Glycols; D013431:Sulfates; D014050:Toluene; C041787:1,3-propanediol; D013429:Sulfatases