title: Induced apoptosis in melanocytes cancer cell and oxidation in biomolecules through deuterium oxide generated from atmospheric pressure non-thermal plasma jet.
abstract: Recently, atmospheric-pressure non-thermal plasma-jets (APPJ) are being for the cancer treatment. However, APPJ still has drawbacks such as efficiency and rise in temperature after treatment. So, in this work, a synergetic agent D2O vapour is attached to APPJ which not only increase the efficiency of plasma source against cancer treatment, but also controlled the temperature during the treatment. OD generated by the combination of D2O + N2 plasma helped in enhancing the efficiency of APPJ. We observed OD induced apoptosis on melanocytes G361 cancer cells through DNA damage signalling cascade. Additionally, we observed that plasma induces ROS, which activated MAPK p38 and inhibits p42/p44 MAPK, leading to cancer cell death. We have also studied DNA oxidation by extracting DNA from treated cancer cell and then analysed the effects of OD/OH/D2O2/H2O2 on protein modification and oxidation. Additionally, we attempted molecular docking approaches to check the action of D2O2 on the apoptosis related genes. Further, we confirmed the formation of OD/OH simultaneously in the solution using optical emission spectroscopy. Moreover, the simultaneous generation of D2O2/H2O2 was detected by the use of confocal Raman spectroscopy and density measurements.
authors.affiliation: Plasma Bioscience Research Center/ Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwon-Ro, Nowon-Gu, Seoul 139-701, Korea.
authors.forename: Naresh
authors.initials: N
authors.lastname: Kumar
authors.affiliation: Plasma Bioscience Research Center/ Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwon-Ro, Nowon-Gu, Seoul 139-701, Korea.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Attri
authors.affiliation: Laboratory of Nanoscale Characterization &Environmental Chemistry, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, Korea.
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Yadav
authors.affiliation: Plasma Bioscience Research Center/ Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwon-Ro, Nowon-Gu, Seoul 139-701, Korea.
authors.forename: Jinsung
authors.initials: J
authors.lastname: Choi
authors.affiliation: Plasma Bioscience Research Center/ Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwon-Ro, Nowon-Gu, Seoul 139-701, Korea.
authors.forename: Eun Ha
authors.initials: EH
authors.lastname: Choi
authors.affiliation: Plasma Bioscience Research Center/ Department of Electrical and Biological Physics, Kwangwoon University, 20 Kwangwon-Ro, Nowon-Gu, Seoul 139-701, Korea.
authors.forename: Han Sup
authors.initials: HS
authors.lastname: Uhm
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001274:Atmospheric Pressure; D045744:Cell Line, Tumor; D004249:DNA Damage; D004273:DNA, Neoplasm / Q000378:metabolism; D017666:Deuterium Oxide / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008544:Melanocytes / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D058626:Plasma Gases / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D058626:Plasma Gases; D017382:Reactive Oxygen Species; D048049:Extracellular Signal-Regulated MAP Kinases; D017666:Deuterium Oxide