title: Genetic mapping of legume orthologs reveals high conservation of synteny between lentil species and the sequenced genomes of Medicago and chickpea.
abstract: Lentil (Lens culinaris Medik.) is a global food crop with increasing importance for food security in south Asia and other regions. Lens ervoides, a wild relative of cultivated lentil, is an important source of agronomic trait variation. Lens is a member of the galegoid clade of the Papilionoideae family, which includes other important dietary legumes such as chickpea (Cicer arietinum) and pea (Pisum sativum), and the sequenced model legume Medicago truncatula. Understanding the genetic structure of Lens spp. in relation to more fully sequenced legumes would allow leveraging of genomic resources. A set of 1107 TOG-based amplicons were identified in L. ervoides and a subset thereof used to design SNP markers for mapping. A map of L. ervoides consisting of 377 SNP markers spread across seven linkage groups was developed using a GoldenGate genotyping array and single SNP marker assays. Comparison with maps of M. truncatula and L. culinaris documented considerable shared synteny and led to the identification of a few major translocations and a major inversion that distinguish Lens from M. truncatula, as well as a translocation that distinguishes L. culinaris from L. ervoides. The identification of chromosome-level differences among Lens spp. will aid in the understanding of introgression of genes from L. ervoides into cultivated L. culinaris, furthering genetic research and breeding applications in lentil.
authors.affiliation: Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada.
authors.forename: Neha
authors.initials: N
authors.lastname: Gujaria-Verma
authors.affiliation: Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada ; Department of Plant Pathology, University of California, Davis Davis, CA, USA.
authors.forename: Sally L
authors.initials: SL
authors.lastname: Vail
authors.affiliation: Department of Plant Pathology, University of California, Davis Davis, CA, USA.
authors.forename: Noelia
authors.initials: N
authors.lastname: Carrasquilla-Garcia
authors.affiliation: Department of Plant Pathology, University of California, Davis Davis, CA, USA.
authors.forename: R Varma
authors.initials: RV
authors.lastname: Penmetsa
authors.affiliation: Department of Plant Pathology, University of California, Davis Davis, CA, USA.
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Cook
authors.affiliation: Bioinformatics, National Centre for Genomic Resources Santa Fe, NM, USA.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Farmer
authors.affiliation: Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada.
authors.forename: Albert
authors.initials: A
authors.lastname: Vandenberg
authors.affiliation: Department of Plant Sciences, University of Saskatchewan Saskatoon, SK, Canada.
authors.forename: Kirstin E
authors.initials: KE
authors.lastname: Bett
keywords: Medicago; legume tentative orthologs; mapping; synteny; translocation; wild lentil