title: Label-free evanescent microscopy for membrane  nano-tomography in living cells.
abstract: We show that through-the-objective evanescent microscopy (epi-EM) is a powerful technique to image membranes in living cells. Readily implementable on a standard inverted microscope, this technique enables full-field and real-time tracking of membrane processes without labeling and thus signal fading. In addition, we demonstrate that the membrane/interface distance can be retrieved with 10 nm precision using a multilayer Fresnel model. We apply this nano-axial tomography of living cell membranes to retrieve quantitative information on membrane invagination dynamics.
authors.affiliation: Institut Langevin, ESPCI ParisTech, CNRS UMR 7587, 1 rue Jussieu, 75231 Paris cedex 05, France. pierre.bon@espci.fr
authors.forename: Pierre
authors.initials: P
authors.lastname: Bon
authors.forename: Thomas
authors.initials: T
authors.lastname: Barroca
authors.forename: Sandrine
authors.initials: S
authors.lastname: Lévèque-Fort
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Fort
mesh_terms: D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D008962:Models, Theoretical; D036103:Nanotechnology / Q000379:methods*; D055095:Optics and Photonics; D012815:Signal Processing, Computer-Assisted; D014057:Tomography, X-Ray Computed / Q000379:methods*