title: The neuron regrowth is associated with the proliferation of neural precursor cells after leukemia inhibitory factor administration following spinal cord injury in mice.
abstract: OBJECTIVE
  To explore whether LIF could promote the proliferation of neural precursor cells (NPCs) and to analyze the correlation between increased NPCs and FluoroGold (FG) labeled neurons in mice after spinal cord injury (SCI).
  
  
  METHODS
  Motor behavior was assessed using Rotarod and Platform Hang tests; neurons in the corticospinal and rubrospinal systems were labeled with FG, NPCs were immustained with nestin-FITC conjugate. The numbers of FG-labeled neurons and NPCs were estimated, and the correlation between FG-labeled neurons and NPCs was assessed.
  
  
  RESULTS
  Mice in the SCI group showed negligible recovery of locomotor behavior; in contrast, mice in the LIF group showed a statically significant improvement. Both FG-labeled neurons and NPCs were significantly increased in the LIF group compared to the SCI group, and this increase in FG-labeled neurons and NPCs showed a clear association above the lesion level.
  
  
  CONCLUSIONS
  LIF could promote locomotive behaviors in mice post-SCI by encouraging the proliferation of NPCs; LIF may in fact be a potential cytokine for the induction of NPCs post-SCI.
authors.affiliation: Capital Medical University, Beijing, 100069, China.
authors.forename: Yubo
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Neurology, Tianjin First Center Hospital, Tianjin Medical University, Tianjin, 300192, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D005260:Female; D053605:Leukemia Inhibitory Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D008124:Locomotion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D058953:Neural Stem Cells / Q000166:cytology* / Q000473:pathology; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology* / Q000473:pathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*
chemical_list: D053605:Leukemia Inhibitory Factor