title: Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis.
abstract: Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total of 95 differentially expressed proteins were identified. Of these, 56 proteins were categorized according to molecular function, cellular component, and biological processes. The most frequent biological processes influenced by the proteins were response to stress, transport, and establishment of localization. Furthermore, a network analysis of the proteins with altered expression in mammary tissues demonstrated that these factors are predominantly involved with binding and structural molecule activities. Vimentin and a-enolase were central "functional hubs" in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands and potential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanisms that protect the mammary glands and promote the pathogenesis of E. coli mastitis.
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Zhao
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Yang
authors.forename: Dong-wei
authors.initials: DW
authors.lastname: Huang
authors.forename: Guang-long
authors.initials: GL
authors.lastname: Cheng
authors.forename: Hui-ling
authors.initials: HL
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002417:Cattle; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000235:genetics / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D008321:Mammary Glands, Animal / Q000276:immunology* / Q000473:pathology; D008414:Mastitis, Bovine / Q000235:genetics* / Q000276:immunology / Q000382:microbiology; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: Escherichia coli; dairy cows; mammary tissue; mastitis; proteome