title: Evaluation of characteristics and in vitro antioxidant properties of RSV loaded hyaluronic acid-DPPC microparticles as a wound healing system.
abstract: Resveratrol (RSV) was incorporated into microparticles by spray drying to treat chronic wounds such as diabetic ulcers. RSV was chosen due to its defense mechanisms as the formation of free radicals delays the healing process. RSV was loaded into microparticles consisting of dipalmitoylphosphatidylcholine (DPPC) and hyaluronic acid (HA), a polysaccharide naturally present within the skin, known to contribute to the healing process. Microparticles were evaluated in terms of production yield, size distribution, encapsulation efficiency, morphology, specific surface area, thermal properties and water content. Spherical and homogenous microparticles (span ≤ 2) in a size range between 20 and 30 μm were obtained with high encapsulation efficiency (≥ 97%). The effect of enzymes (hyaluronidase, phospholipase and lipase) on RSV release showed a dose-dependent pattern followed by a slow release stage. Cytotoxicity/proliferation and oxidative stress parameters (glutathione, oxidized glutathione, glutathione peroxidase, malondialdehyde, superoxide dismutase) obtained from human dermal fibroblast cell cultures revealed that formulations increased cell proliferation and the presence of RSV decreased oxidation in cells. RSV-loaded HA-DPPC microparticles appear as a promising formulation for wound healing due to synergistic effect of the ingredients.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, 35100, Turkey.
authors.forename: İpek
authors.initials: İ
authors.lastname: Eroğlu
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, 35100, Turkey.
authors.forename: Evren H
authors.initials: EH
authors.lastname: Gökçe
authors.affiliation: Université Paris-Sud, Institut Galien Paris-Sud, UMR CNRS 8612, Faculté de Pharmacie, Rue JB Clément, 92296, Châtenay-Malabry, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Tsapis
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, 35100, Turkey.
authors.forename: Sakine Tuncay
authors.initials: ST
authors.lastname: Tanrıverdi
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Ege, Izmir, 35100, Turkey.
authors.forename: Göksel
authors.initials: G
authors.lastname: Gökçe
authors.affiliation: Université Paris-Sud, Institut Galien Paris-Sud, UMR CNRS 8612, Faculté de Pharmacie, Rue JB Clément, 92296, Châtenay-Malabry, France.
authors.forename: Elias
authors.initials: E
authors.lastname: Fattal
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Ege, Izmir, 35100, Turkey. Electronic address: ozgen.ozer@ege.edu.tr.
authors.forename: Özgen
authors.initials: Ö
authors.lastname: Özer
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D000077185:Resveratrol; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D015060:1,2-Dipalmitoylphosphatidylcholine; D006820:Hyaluronic Acid; D000077185:Resveratrol
keywords: Antioxidant.; Hyaluronic acid; Microparticles; Resveratrol; Wound healing