title: Cell-wall polysaccharide composition and glycanase activity of Silene vulgaris callus transformed with rolB and rolC genes.
abstract: The aim of this research is to investigate the effects of the Agrobacterium rhizogenes rol genes on the composition of cell-wall polysaccharides and glycanase activity in the campion callus. The expression of the rolC gene reduces the yield of campion pectin, while the expression of the rolB or rolC gene inhibits the volumetric production of both pectin and intracellular arabinogalactan. The rol genes are involved in regulating the activity of glycanases and esterases, thereby contributing to the modification of polysaccharide structures, their molecular weight (Mw) and the degree of pectin methyl esterification (DE). The increase in pectin arabinose residue appears to be connected to a decrease in intracellular and extracellular α-l-arabinofuranosidase activity in transgenic campion calluses. In transgenic calluses expressing the rolB and rolC genes, the increase in pectin galactose residue is likely due to a decrease in β-galactosidase activity. The decrease in the Mw of pectin and its d-galacturonic acid content appears to be connected to an increase in extracellular polygalacturonase activity. Finally, the increase in pectinesterase activity causes a decrease in the DE of pectin. Thus, the expression of rolB and rolC genes in campion callus has a considerable effect on pectin's sugar composition, DE and Mw, while it appears to have an insignificant influence on intracellular and extracellular arabinogalactans.
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., 167982 Syktyvkar, Russia. Electronic address: gunter-ea@mail.ru.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Günter
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159, Prospect 100-letija, 690022 Vladivostok, Russia; Far Eastern Federal University, 8, Sukhanova str., 690022 Vladivostok, Russia.
authors.forename: Yury N
authors.initials: YN
authors.lastname: Shkryl
authors.affiliation: Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya str., 167982 Syktyvkar, Russia.
authors.forename: Oxana V
authors.initials: OV
authors.lastname: Popeyko
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159, Prospect 100-letija, 690022 Vladivostok, Russia.
authors.forename: Galina N
authors.initials: GN
authors.lastname: Veremeichik
authors.affiliation: Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, 159, Prospect 100-letija, 690022 Vladivostok, Russia; Far Eastern Federal University, 8, Sukhanova str., 690022 Vladivostok, Russia.
authors.forename: Victor P
authors.initials: VP
authors.lastname: Bulgakov
mesh_terms: D060054:Agrobacterium / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry*; D005685:Galactans / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D005685:Galactans; D011134:Polysaccharides; D010368:Pectins; D006026:Glycoside Hydrolases; C020668:glycanase; C005653:arabinogalactan
keywords: Agrobacterium rhizogenes; Callus; Cell-wall polysaccharides; Glycanase; Silene vulgaris; rol Genes