title: A group-specific sequence-based typing approach for HLA-DQA1.
abstract: An HLA-DQA1 sequence-based typing method reliant upon group-specific amplification to achieve an unambiguous second-field DQA1 typing assignment is presented. Method validation, using 51 reference DNA samples covering 21 different DQA1 alleles, showed 100% concordance with the reference types. This typing strategy has several important uses including identifying DQA1 mismatches in kidney donor/recipient pairs to inform patient DQ antibody assignments.
authors.affiliation: Welsh Transplantation and Immunogenetics Laboratory, Welsh Blood Service, Pontyclun, Wales, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Lemin
authors.forename: J
authors.initials: J
authors.lastname: Johnson
authors.forename: C
authors.initials: C
authors.lastname: Darke
mesh_terms: D001483:Base Sequence; D059848:HLA-DQ alpha-Chains / Q000235:genetics* / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D016030:Kidney Transplantation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen