title: Interleukin-6 c.-174G>C Polymorphism and Periodontitis in a Brazilian Population.
abstract: Aim. Periodontitis is an inflammatory disease that affects the teeth supporting structures, triggered by periodontal pathogens, and is influenced by environmental and genetic factors. Genes encoding molecules related to the immune response, such as cytokine, are the main candidates for polymorphisms analysis and may be possibly associated with this pathology. A G/C promoter polymorphism on the IL6 gene has been shown to affect basal IL-6 levels. The aim of this study was to investigate the association between the IL6 c.-174G>C polymorphism and periodontitis in individuals from Vitória da Conquista, Bahia, Brazil. Material and Methods. Three hundred and thirty individuals (134 cases, 196 controls) were genotyped for the IL6 c.-174G>C by MS-PCR technique. Concentrations of salivary IL-6 were determined by ELISA method. Results. The IL6 c.-174G>C polymorphism was associated with periodontitis when comparing the distribution of genotypes between patients with periodontitis and control subjects. The GC genotype appeared as a protective factor for periodontitis. Results showed increased levels of salivary IL-6 in periodontitis patients. Nevertheless, there was no relationship between the concentrations of IL-6 and genotypes when comparing the case and control groups. Conclusions. Our data indicate an association between IL6 c.-174G>C polymorphism and periodontitis and showed that IL-6 may be considered an important marker for periodontitis.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Gabriela Teixeira
authors.affiliation: Laboratory of Cellular and Molecular Biology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
authors.forename: Samir Andrade
authors.initials: SA
authors.lastname: Mendonça
authors.affiliation: Laboratory of Cellular and Molecular Biology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
authors.forename: Kamilla
authors.initials: K
authors.lastname: Menezes Oliveira
authors.affiliation: Health Sciences Center, Federal University of Bahia Recôncavo, 44380-000 Santo Antonio de Jesus, BA, Brazil.
authors.forename: Djanilson
authors.initials: D
authors.lastname: Barbosa Dos Santos
authors.affiliation: Laboratory of Microbiology and Immunology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
authors.forename: Lucas
authors.initials: L
authors.lastname: Miranda Marques
authors.affiliation: Laboratory of Cellular and Molecular Biology, Multidisciplinary Health Institute, Federal University of Bahia, 45029-094 Vitória da Conquista, BA, Brazil.
authors.forename: Maise
authors.initials: M
authors.lastname: Mendonça Amorim
authors.affiliation: NUPEM, Federal University of Rio de Janeiro, Avenida São José do Barreto, 764 São José do Barreto, 27965-045 Macaé, RJ, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: de Souza Gestinari