title: The effects of dietary soybean isoflavones on growth, innate immune responses, hepatic antioxidant abilities and disease resistance of juvenile golden pompano Trachinotus ovatus.
abstract: The present study was conducted to investigate the effects of dietary soybean isoflavones (SI) supplementation on growth performance, innate immune responses, hepatic antioxidant abilities, heat shock protein 70 (HSP70) gene expression and resistance to the pathogen Vibrio harveyi in Trachinotus ovatus. A basal diet was supplemented with SI at 0, 10, 20, 40, 60, 80 mg kg(-1) feed for 8 weeks. Significantly maximum weight gain (WG) and specific growth rate (SGR) were observed in treatment with 40 mg kg(-1) SI supplement (P < 0.05). Feed conversion ratio (FCR), feeding rate (FR) and survival rate were not significantly different among treatments. Fish fed a diet with 40 mg kg(-1) SI showed significant increase in plasma total protein content, complement 3 content, lysozyme activity as well as respiratory burst activity, but decrease in alanine aminotransferase and aspartate aminotransferase activities (P < 0.05). Increased plasma alkaline phosphatase activity, hepatic total antioxidative capacity, catalase activity and superoxide dismutase activity were also noticed in fish fed SI at 40 or 60 mg kg(-1) (P < 0.05). On the contrary, the lowest hepatic malondialdehyde (MDA) content was observed in fish fed SI at 40 mg kg(-1) (P < 0.05). Compared with the control, the relative level of HSP70 mRNA in fish fed SI at 40-80 mg kg(-1) were significantly increased, respectively (P < 0.05). After challenge with V. harveyi, significant higher post-challenge survival was observed in fish fed diets with 40-80 mg kg(-1) SI supplement than that in control group (P < 0.05). These results indicated that dietary intake containing SI could enhance the immune ability of fish and improve its resistance to infection by V. harveyi. Especially supplementation with 40 mg kg(-1) SI to the fish for 8 weeks showed remarkable improvement in the growth, non-specific immune responses, hepatic antioxidant abilities and HSP70 gene expression.
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China.
authors.forename: Chuanpeng
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China. Electronic address: linheizhao@163.com.
authors.forename: Heizhao
authors.initials: H
authors.lastname: Lin
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, PR China. Electronic address: gexp@ffrc.cn.
authors.forename: Xianping
authors.initials: X
authors.lastname: Ge
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Niu
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Lixiong
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yu
authors.affiliation: Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture, The South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Tan
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements / Q000032:analysis; D060467:Disease Resistance / Q000187:drug effects*; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate / Q000187:drug effects*; D007529:Isoflavones / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010473:Perciformes / Q000254:growth & development; D013025:Glycine max / Q000737:chemistry*; D014733:Vibrio / Q000502:physiology; D014735:Vibrio Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000975:Antioxidants; D007529:Isoflavones
keywords: Growth; Innate immunity; Soybean isoflavones; Trachinotus ovatus; Vibrio harveyi