title: 3D electrospun silk fibroin nanofibers for fabrication of artificial skin.
abstract: Tissue-engineered skin substitutes such as nanofibers from traditional electrospinning may offer an effective therapeutic option for the treatment of patients suffering from skin damages such as burns and diabetic ulcers. However, it is generally difficult for cells to infiltrate the nanofibers due to their small pore size and sheets-like appearance. In the present study, a facile and efficient strategy has successfully been introduced that can produce 3D silk fibroin nanofibers, obviating an intrinsic limitation of traditional and salt-leaching electrospinning by introducing cold-plate electrospinning. The cell attachment and infiltration studies indicated the use of 3D nanofiber scaffolds by cold-plate electrospinning as a potential candidate to overcome intrinsic barriers of electrospinning techniques. The 3D nanofiber scaffolds using this technique presented a high porosity with controlled thickness and an easy contouring of facial shape; these properties can contribute to the ideal candidate for artificial skin reconstruction. From the clinical editor: Electrospun nanofibers are considered as promising scaffolds for tissue engineering due to extracellular matrix mimicking factor resulting in a controllable 3D nanofibrous form. The cold-plate electrospinning technique can facilitate the fabrication of these biomaterials to create structures that could resemble the dermis.
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Faheem A
authors.initials: FA
authors.lastname: Sheikh
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Hyung Woo
authors.initials: HW
authors.lastname: Ju
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Bo Mi
authors.initials: BM
authors.lastname: Moon
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Ok Joo
authors.initials: OJ
authors.lastname: Lee
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Dong-Kyu
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, South Korea; Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, Hallym University, Chuncheon, South Korea. Electronic address: hlpch@paran.com.
authors.forename: Chan Hum
authors.initials: CH
authors.lastname: Park
mesh_terms: D005349:Fibroins / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D017768:Skin, Artificial
chemical_list: D005349:Fibroins
keywords: 3D nanofibers; Artificial skin; Cold-plate electrospinning; Full-thickness scaffolds