title: Adipose-derived stem cells: selecting for translational success.
abstract: We have witnessed a rapid expansion of in vitro characterization and differentiation of adipose-derived stem cells, with increasing translation to both in vivo models and a breadth of clinical specialties. However, an appreciation of the truly heterogeneous nature of this unique stem cell group has identified a need to more accurately delineate subpopulations by any of a host of methods, to include functional properties or surface marker expression. Cells selected for improved proliferative, differentiative, angiogenic or ischemia-resistant properties are but a few attributes that could prove beneficial for targeted treatments or therapies. Optimizing cell culture conditions to permit re-introduction to patients is critical for clinical translation.
authors.affiliation: Blond McIndoe Laboratories, Institute of Inflammation & Repair, School of Medicine, University of Manchester, M13 9PT, UK.
authors.forename: Kavan S
authors.initials: KS
authors.lastname: Johal
authors.forename: Vivien C
authors.initials: VC
authors.lastname: Lees
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Reid
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D019651:Plastic Surgery Procedures; D033581:Stem Cell Transplantation / Q000009:adverse effects; D013234:Stem Cells / Q000166:cytology*; D057170:Translational Research, Biomedical / Q000379:methods*
keywords: adipose; adipose-derived stem cells; clinical translation; cluster of differentiation markers; fat grafting; serum-free media; stromal vascular fraction; subpopulation selection