title: Molecular cloning of a C-type lectin with one carbohydrate recognition domain from Fenneropenaeus merguiensis and its expression upon challenging by pathogenic bacterium or virus.
abstract: Lectins, one type of pattern recognition proteins, play important roles in an innate immunity of crustaceans including shrimp. A new C-type lectin designated FmLC1 was cloned from the hepatopancreas of banana shrimp Fenneropenaeus merguiensis by procedures of PCR and 5' and 3' rapid amplification of cDNA ends (RACE). The full-length cDNA is composed of 706bp with a single open reading frame of 477bp, encoding a peptide of 158 amino acid residues. Its deduced amino acid sequence comprises a putative signal peptide of 17 amino acids and has an estimated molecular mass of 17,934Da with a theoretical pI of 4.46. The primary sequence of FmLC1 contains a single carbohydrate recognition domain (CRD) with an EPS (Glu-Pro-Ser) motif and one Ca(2+) binding site, stabilized by two disulfide bonds. FmLC1 mRNA was detected to express specifically in the hepatopancreas, a master organ in shrimp. Its expression in the hepatopancreas was up-regulated to reach the maximum at 12 or 48h following challenge of shrimp with Vibrio harveyi or white spot syndrome virus, respectively. These results suggest that FmLC1 may participate in recognition of invading pathogens such as bacteria and viruses, and play roles in the immune response of shrimp even at different stages of the clearance of pathogens.
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
authors.forename: Supattra
authors.initials: S
authors.lastname: Thepnarong
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
authors.forename: Phanthipha
authors.initials: P
authors.lastname: Runsaeng
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
authors.forename: Onnicha
authors.initials: O
authors.lastname: Rattanaporn
authors.affiliation: Department of Biochemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand. Electronic address: prapaporn.u@psu.ac.th.
authors.forename: Prapaporn
authors.initials: P
authors.lastname: Utarabhand
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003001:Cloning, Molecular; D005786:Gene Expression Regulation; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000502:physiology; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D037181:Lectins, C-Type
keywords: Banana shrimp; EPS motif; Fenneropenaeus merguiensis; Single-CRD lectin; Vibrio harveyi; WSSV