title: Transposable elements and two other molecular markers as typing tools for the genus Paracoccidioides.
abstract: Studies comparing Paracoccidioides brasiliensis and Paracoccidioides lutzii have shown that these fungi have significant genomic differences that may have implications in the clinical manifestation, diagnosis, and treatment of paracoccidioidomycosis caused by them. Thus, molecular typing methods are required that can distinguish between various species of Paracoccidioides. The aim of this study was to explore the potential use as molecular markers of the transposable elements Trem A-H recently identified and characterized in the genus Paracoccidioides as a means of differentiating the species. We take advantage of the abundance and distribution of these transposons in the Paracoccidioides genomes to develop a simple and highly reproducible polymerase chain reaction (PCR)-based technique. Furthermore we compare the performance of this test with two other molecular markers already in use to identify these fungi.
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Fernanda Lourenço
authors.initials: FL
authors.lastname: Alves
authors.affiliation: Departamento de Patologia, Centro Biomédico, Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
authors.forename: Mariceli Araújo
authors.initials: MA
authors.lastname: Ribeiro
authors.affiliation: Faculdade de Ciências Médicas, Universidade Federal do Mato Grosso, Mato Grosso, MT, Brazil.
authors.forename: Rosane Christine
authors.initials: RC
authors.lastname: Hahn
authors.affiliation: Instituto de Ciências Biológicas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, Brazil.
authors.forename: Marcus
authors.initials: M
authors.lastname: de Melo Teixeira
authors.affiliation: Disciplina de Biologia Celular, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
authors.forename: Zoilo Pires
authors.initials: ZP
authors.lastname: de Camargo
authors.affiliation: Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Patrícia Silva
authors.initials: PS
authors.lastname: Cisalpino
authors.affiliation: Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil marjoriemarini@gmail.com.
authors.forename: Marjorie Mendes
authors.initials: MM
authors.lastname: Marini
mesh_terms: D004251:DNA Transposable Elements / Q000235:genetics*; D006801:Humans; D058889:Molecular Typing / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D010228:Paracoccidioides / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010229:Paracoccidioidomycosis / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004251:DNA Transposable Elements
keywords: Paracoccidioides; hsp70; microsatellites; molecular typing; transposons