title: Biological Matrix Effects in Quantitative Tandem Mass Spectrometry-Based Analytical Methods: Advancing Biomonitoring.
abstract: The ability to quantify levels of target analytes in biological samples accurately and precisely in biomonitoring involves the use of highly sensitive and selective instrumentation such as tandem mass spectrometers and a thorough understanding of highly variable matrix effects. Typically, matrix effects are caused by co-eluting matrix components that alter the ionization of target analytes as well as the chromatographic response of target analytes, leading to reduced or increased sensitivity of the analysis. Thus, before the desired accuracy and precision standards of laboratory data are achieved, these effects must be characterized and controlled. Here we present our review and observations of matrix effects encountered during the validation and implementation of tandem mass spectrometry-based analytical methods. We also provide systematic, comprehensive laboratory strategies needed to control challenges posed by matrix effects in order to ensure delivery of the most accurate data for biomonitoring studies assessing exposure to environmental toxicants.
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: Parinya
authors.initials: P
authors.lastname: Panuwet
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.|c ORISE, Centers for Disease Control and Prevention , Atlanta , Georgia , USA.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Hunter
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: Priya E
authors.initials: PE
authors.lastname: D'Souza
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.|b Department of Chemistry , Emory University , Atlanta , Georgia , USA.
authors.forename: Xianyu
authors.initials: X
authors.lastname: Chen
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.|b Department of Chemistry , Emory University , Atlanta , Georgia , USA.
authors.forename: Samantha A
authors.initials: SA
authors.lastname: Radford
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: Jordan R
authors.initials: JR
authors.lastname: Cohen
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: M Elizabeth
authors.initials: ME
authors.lastname: Marder
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: Kostya
authors.initials: K
authors.lastname: Kartavenka
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.|b Department of Chemistry , Emory University , Atlanta , Georgia , USA.
authors.forename: P Barry
authors.initials: PB
authors.lastname: Ryan
authors.affiliation: a Department of Environmental Health , Rollins School of Public Health, Emory University , Atlanta , Georgia , USA.
authors.forename: Dana Boyd
authors.initials: DB
authors.lastname: Barr
mesh_terms: D004781:Environmental Exposure / Q000032:analysis*; D015386:Hazardous Substances / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D015386:Hazardous Substances
keywords: Analytical method development; biological analysis; biomonitoring; matrix effects; tandem mass-spectrometry