title: Transcriptome changes upon in vitro challenge with Mycobacterium bovis in monocyte-derived macrophages from bovine tuberculosis-infected and healthy cows.
abstract: As innate immune cells, macrophages are expected to respond to mycobacterial infection equally in both Mycobacterium bovis-infected cows and healthy cows. We previously found that monocyte-derived macrophages (MDMs) from M. bovis-infected cows respond differently than MDMs from healthy cows when exposed to in vitro M. bovis challenge. We have now used the Agilent™ Bovine Gene Expression Microarray to examine transcriptional differences between these MDMs. At a high multiplicity of infection (10), in vitro challenge led to changes in several thousands of genes, with dysregulation at multiple orders of magnitude. For example, significant changes were seen for colony stimulating factor 3 (granulocyte) (CSF3), colony stimulating factor 2 (granulocyte-macrophage) (CSF2), and chemokine (C-C motif) ligand 20 (CCL20). Classical macrophage activation was also observed, although to a lesser degree in interleukin 12 (IL12) expression. For macrophages, kallikrein-related peptidase 12 (KLK12) and protease, serine, 2 (trypsin 2) (PRSS2), as well as a secreted protein, acidic, cysteine-rich (osteonectin) (SPARC)-centered matricellular gene network, were differentially expressed in infected animals. Finally, global transcriptome fold-changes caused by in vitro challenge were higher in healthy cows than in tuberculosis-positive cows, suggesting that healthy macrophages responded marginally better to in vitro infection. Macrophages from healthy and already infected animals can both be fully activated during M. bovis infection, yet there are differences between these macrophages: distinct expression pattern in matricellular proteins, and their different responses to in vitro infection.
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Jingjun
authors.initials: J
authors.lastname: Lin
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Zhao
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institut de Génétique et Microbiologie, Université Paris-Sud 11, 91405 Orsay, France.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yin
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.affiliation: The State Key Lab of Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National TSE Lab, College of Veterinary Medicine, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China. Electronic address: zhouxm@cau.edu.cn.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002417:Cattle; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism* / Q000382:microbiology; D009163:Mycobacterium bovis / Q000502:physiology*; D040081:Protein Array Analysis; D059467:Transcriptome / Q000276:immunology*; D014380:Tuberculosis, Bovine / Q000378:metabolism*
keywords: Bovine tuberculosis; Microarray; Monocyte-derived macrophages; Mycobacterium bovis; Pathway analysis