title: [Detection of transforming growth factor-β1 in colorectal cancer and its clinical significance].
abstract: OBJECTIVE
  To investigate the role of transforming growth factor β1 (TGF-β1) in patients with colorectal cancer.
  
  
  METHODS
  Fresh peripheral blood were obtained from 50 patients (before surgery and at least one week after surgery) and 25 healthy donors in the morning. Fresh colorectal cancer tissues and the adjacent tissues (at least 5 cm from the tumor site) were obtained from patients undergoing tumor resection. The expression levels of TGF-β1 in the blood and tissue specimens were determined using ELISA.
  
  
  RESULTS
  The plasma levels of TGF-β1 in patients with colorectal cancer were significantly higher than those in the healthy donors, and decreased after the surgery (P<0.05). The tumor tissues expressed higher levels of TGF-β1 than the adjacent tissues from both CEA-negative and -positive patients. The plasma level of TGF-β1 in the patients were positively correlated with the tumor size and clinical tumor stage (P<0.05).
  
  
  CONCLUSIONS
  TGF-β1 combined with CEA can provide important information for the diagnosis, prognostic assessment and prediction of recurrence in patients with colorectal cancer, and may provide new insights for anti-TGF-β1-based tumor immune therapeutic strategies.
authors.affiliation: Department of Coloproctological Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.E-mail: drhuang5413@hotmail.com.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Huang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Cao
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Sen
authors.initials: S
authors.lastname: Zhang
authors.forename: Leichang
authors.initials: L
authors.lastname: Zhang
authors.forename: Junxian
authors.initials: J
authors.lastname: Long
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D006801:Humans; D009364:Neoplasm Recurrence, Local; D011379:Prognosis; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D053773:Transforming Growth Factor beta1