title: Molecular analysis and antimicrobial susceptibility of enterotoxigenic Escherichia coli from diarrheal patients.
abstract: A total of 123 enterotoxigenic Escherichia coli (ETEC) isolates from diarrheal patients from June to December 2012 in Shanghai, China, were examined to determine their genetic relatedness using multilocus sequence typing and pulsed-field gel electrophoresis (PFGE) and for the presence of virulence genes and antimicrobial susceptibility. Twenty-nine sequence types (STs) and 63 PFGE patterns were identified, and results from the 2 subtyping methods correlated well. The 12 isolates of PFGE cluster B all belonged to ST-2332 and were associated with nosocomial neonatal diarrhea. Isolates of a cluster usually had the same set of virulence factors, whereas isolates of different PFGE clusters carried diverse combinations of virulence determinants. Isolates belonging to ST-2332 and ST-182 (n=9) were resistant to at least 6 antimicrobials. Our findings highlighted the need of active surveillance programs for infectious diseases collecting data at both epidemiological and genetic levels that can detect high-risk lineages of pathogens in order to rapidly identify disease outbreaks.
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Haijian
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Dai
authors.initials: D
authors.lastname: Kuang
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Ju
authors.affiliation: The Changning District Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: The Changning District Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Guo
authors.affiliation: The Putuo District Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Municipal Ke-Ma-Jia Technology Center for Microbiology, Shanghai, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Shanghai Municipal Ke-Ma-Jia Technology Center for Microbiology, Shanghai, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Shi
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
authors.forename: Huiming
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China. Electronic address: xbxu@scdc.sh.cn.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; Department of Food Science and Nutrition, University of Maryland, College Park, MD, USA. Electronic address: jmeng@umd.edu.
authors.forename: Jianghong
authors.initials: J
authors.lastname: Meng
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000453:epidemiology* / Q000382:microbiology; D054307:Enterotoxigenic Escherichia coli / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058889:Molecular Typing; D037521:Virulence Factors / Q000235:genetics; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors
keywords: Antimicrobial susceptibility; ETEC; MLST; PFGE; Virulence gene