title: Antimicrobial and cytotoxic isopimarane diterpenoid glycosides from Microlepia pilosissima Ching.
abstract: A phytochemical investigation of the EtOH extract of the dry fronds of Microlepia pilosissima Ching afforded four new isopimarane diterpene glycosides, 3α-O-α-L-rhamnopyranosyl-7β-O-β-D-fucopyranosyl-ent-iospimara-8(14),15-diene (1), 3α-O-[2-O-acetyl-α-L-rhamnopyranosyl]-7β-O-β-D-fucopyranosyl-ent-iospimara-8(14),15-diene (2), 3α-O-[β-D-glucopyranosyl-(1→2)-α-L-rhamnopyranosyl]-7β-O-β-D-fucopyranosyl-ent-iospimara-8(14),15-diene (3) and 3α-O-[β-D-fucopyranosyl-(1→2)-α-L-rhamnopyranosyl]-7β-O-β-D-fucopyranosyl-ent-iospimara-8(14),15-diene (4) as well as their aglycone, 3α,7β-dihydroxy-ent-iospimara-8(14),15-diene (5). Their structures were characterized by spectroscopic methods, including 1D-NMR, 2D-NMR, and HR-ESI-MS. The isolated compounds were evaluated in vitro for antimicrobial properties against three pathogen fungi and two oral pathogens and cytotoxicities against eight tumor cell lines. As a result, compounds 1-4 appeared to be promising antimicrobial potential and possessed moderate cytotoxic activities against the tested tumor cell lines.
authors.affiliation: College of Biological Resources and Environment Science, Qujing Normal University, Qujing 655011, Yunnan, China. Electronic address: hujiang@ustc.edu.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Pharmacy, 455 Hospital of People's Liberation Army, Shanghai 200052, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Physics and Electronic Engineering, Qujing Normal University, Qujing 655011, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Biological Resources and Environment Science, Qujing Normal University, Qujing 655011, Yunnan, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Mao
authors.affiliation: College of Biological Resources and Environment Science, Qujing Normal University, Qujing 655011, Yunnan, China.
authors.forename: YongMao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Biological Resources and Environment Science, Qujing Normal University, Qujing 655011, Yunnan, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Shi
mesh_terms: D045784:Abietanes / Q000737:chemistry* / Q000302:isolation & purification; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000302:isolation & purification; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D029624:Ferns / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D015394:Molecular Structure; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D006027:Glycosides
keywords: Antimicrobial; Cytotoxicity; Dennstaedtiaceae; Isopimarane diterpene glycosides; Microlepia pilosissima Ching