title: Vascular endothelial growth factor genotypes and haplotypes contribute to the susceptibility of obstructive sleep apnea syndrome.
abstract: BACKGROUND
  To investigate whether VEGF polymorphisms (-460 T/C, +405 G/C, and +936 C/T)/haplotypes influence the susceptibility of obstructive sleep apnea (OSA).
  
  
  METHODS
  A prospective case-control study was conducted to evaluate the genetic effects of VEGF polymorphisms on the development of OSA. 150 patients and 225 healthy controls were recruited for this study and their genotypes were determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The odds ratios (OR) and 95% confidence intervals (CI) were calculated by logistic regression analysis.
  
  
  RESULTS
  Our study showed that the -460 C allele (C vs. T: OR = 1.95, 95% CI = 1.38-2.76) and +936 T allele (T vs. C: OR = 1.48, 95% CI = 1.02-2.15) were associated with an increased OSA risk, whereas +405 C allele was associated with a decreased susceptibility to OSA (C vs. G: OR = 0.61, 95% CI = 0.45-0.83). Compared with the most common haplotype CCT, CGC (OR = 2.22, 95% CI = 1.19-4.13) and TGC (OR = 3.83, 95% CI = 1.56-9.40) were associated with a significantly increased risk of OSA.
  
  
  CONCLUSIONS
  These observations implied that VEGF gene polymorphisms might be associated with the susceptibility to OSA. These results need to be validated by other independent studies, especially in diverse ethnic populations.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China; Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China.
authors.forename: Qunli
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China; Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Lv
authors.affiliation: Zhejiang University School of Medicine, Hangzhou, 310058, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China.
authors.forename: Shifang
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China.
authors.forename: Zhongbo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Huahao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of School of Medicine, Ningbo University, Ningbo, 315020, China.
authors.forename: Zaichun
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D020181:Sleep Apnea, Obstructive / Q000235:genetics*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A