title: Ultrasensitive electrochemical detection of breast cancer cells based on DNA-rolling-circle-amplification-directed enzyme-catalyzed polymerization.
abstract: An ultrasensitive cytosensor based on DNA-rolling-circle-amplification-directed enzyme-catalyzed polymerization is demonstrated. As a proof of concept, the cytosensor shows excellent sensitivity for MCF-7 cell detection with a lower detection limit of 12 cells per mL.
authors.affiliation: Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi 710069, China. zhengjb@nwu.edu.cn.
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Sheng
authors.forename: Ni
authors.initials: N
authors.lastname: Cheng
authors.forename: Wushuang
authors.initials: W
authors.lastname: Bai
authors.forename: Jianbin
authors.initials: J
authors.lastname: Zheng
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000378:metabolism; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D001943:Breast Neoplasms / Q000473:pathology*; D002469:Cell Separation; D004247:DNA / Q000235:genetics*; D004563:Electrochemistry; D004798:Enzymes / Q000378:metabolism*; D006801:Humans; D057230:Limit of Detection; D061986:MCF-7 Cells; D021141:Nucleic Acid Amplification Techniques; D058105:Polymerization
chemical_list: D000814:Aniline Compounds; D052157:Aptamers, Nucleotide; D004798:Enzymes; C416807:polyaniline; D004247:DNA