title: Genetic diversity of Clavispora lusitaniae isolated from Agave fourcroydes Lem, as revealed by DNA fingerprinting.
abstract: This study characterized Clavispora lusitaniae strains isolated from different stages of the processing and early fermentation of a henequen (Agave fourcroydes) spirit produced in Yucatan, Mexico using a molecular technique. Sixteen strains identified based on morphological features, obtained from different substrates, were typed molecularly. Nine different versions of the divergent D1/D2 domain of the large-subunit ribosomal DNA sequence were identified among the C. lusitaniae strains. The greatest degree of polymorphism was found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% of the isolates. This study provides significant insight into the genetic diversity of the mycobiota present during the henequen fermentation process, especially that of C. lusitaniae, for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealing olymorphisms between isolates of this species.
authors.affiliation: Laboratorio GeMBio, Centro de Investigación Científica de Yucatán A.C., Calle 43 # 130, Col. Chuburná de Hidalgo, Mérida, Yucatán, 97200, México.
authors.forename: Daisy
authors.initials: D
authors.lastname: Pérez-Brito
authors.forename: Anuar
authors.initials: A
authors.lastname: Magaña-Alvarez
authors.forename: Patricia
authors.initials: P
authors.lastname: Lappe-Oliveras
authors.forename: Alberto
authors.initials: A
authors.lastname: Cortes-Velazquez
authors.forename: Claudia
authors.initials: C
authors.lastname: Torres-Calzada
authors.forename: Teófilo
authors.initials: T
authors.lastname: Herrera-Suarez
authors.forename: Alfonso
authors.initials: A
authors.lastname: Larqué-Saavedra
authors.forename: Raul
authors.initials: R
authors.lastname: Tapia-Tussell
mesh_terms: D027882:Agave / Q000382:microbiology*; D000434:Alcoholic Beverages / Q000382:microbiology*; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal; D005285:Fermentation; D014644:Genetic Variation; D008800:Mexico; D064307:Microbiota; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal