title: New emerging bio-catalysts design in biotransformations.
abstract: The development of new and successful biotransformation processes of key interest in medicinal and pharmaceutical chemistry involves creating new biocatalysts with improved or even new activities and selectivities. This review emphasizes the new emerging developed strategies to achieve this goal, site-selective chemical modification of enzymes using tailor-made peptides, specific insertion of metals or organometallic complexes into proteins producing bio-catalysts with multiple activities and computational design for creating evolved artificial enzymes with non-natural synthetic catalytic activities.
authors.affiliation: Departamento of Biocatalisis, Instituto de Catalisis (CSIC), Marie Curie 2, Cantoblanco 28049, Madrid, Spain. Electronic address: josempalomo@icp.csic.es.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Palomo
authors.affiliation: Departamento of Biocatalisis, Instituto de Catalisis (CSIC), Marie Curie 2, Cantoblanco 28049, Madrid, Spain.
authors.forename: Marco
authors.initials: M
authors.lastname: Filice
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology; D001711:Biotransformation; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering; D013237:Stereoisomerism
chemical_list: D004798:Enzymes
keywords: Artificial metalloenzymes; Bionanohybrids; Biotransformations; C–C bond reaction; Deacetylation; Regioselectivity; Semisynthetic enzymes