title: Identification of new phytoconstituents and antimicrobial activity in stem bark of Mangifera indica (L.).
abstract: Mangifera indica, commonly called mango or amra belonging to a family of Anacardiaceae, is an important medicinal plant widely used in a variety of Ayurvedic preparations. Extract of its bark, leaves, flowers and kernels are being extensively used for curing various chronic diseases. Mango wood is used in yagya as base fire through which medicated smoke is generated. Three new compounds have been isolated from methanolic and hexane extracts of stem bark: 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl)ester and 9,12-tetradecadiene-1-ol-acetate from the hexane extract and 3-chloro-N-(2-phenylethyl) propanamide from the methanolic extract. These were first separated by thin layer chromatography and later in a silica gel column and identified by characteristic infrared bands corresponding to respective functional groups. The compounds were further confirmed on the basis of GC-MS fragmentation pattern after comparing the data with NIST mass spectral database. All three compounds exhibited antimicrobial activity due to triterpenoids and flavonoids. Elemental analyses by INAA show it to be enriched in essential nutrient elements such as Ca, Fe, K, Mn and Zn which all play an important role in enzymatic processes.
authors.affiliation: Department of Oriental Studies, Dev Sanskrit University, Haridwar 249411, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Singh
authors.affiliation: Department of Botany, DAV College, Kanpur 208001, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Roorkee 247667, India.
authors.forename: R S
authors.initials: RS
authors.lastname: Maharia
authors.affiliation: Institute of Nuclear Science and Technology, Amity University, Sector 125, Noida 201313, India. Electronic address: amarnath943@yahoo.com.
authors.forename: A N
authors.initials: AN
authors.lastname: Garg
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D008401:Gas Chromatography-Mass Spectrometry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D031022:Mangifera / Q000737:chemistry*; D008515:Medicine, Ayurvedic; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000302:isolation & purification*; D018547:Plant Stems / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; C501277:Mangifera indica extract; D010936:Plant Extracts
keywords: Chemical constituents; Column chromatography; GC–MS; Mangifera indica; Thin layer chromatography