title: Association detection between genetic variants in the microRNA binding sites of toll-like receptors signaling pathway genes and bladder cancer susceptibility.
abstract: Bladder cancer (BCa) is the second most common urological malignancy, and the incidence of BCa has dramatically increased recently. Various toll-like receptors (TLRs) signaling pathway proteins were proven to be associated with BCa susceptibility. However, the effect of genetic variants in TLRs signaling pathway genes on risk of BCa has not been elucidated clearly. Previous studies mainly focused on the coding region of target genes, while in this study, polymorphisms in the non-coding region, microRNA (miRNA) binding sites were investigated as potential targets. We used bioinformatics approach to screen 100 BCa related TLRs signaling pathway genes. Candidate polymorphisms were select in this region and 8 polymorphisms were confirmed. Rs72552316, located at the 3'UTR of the TLR7 gene, exhibited significant association with risk of BCa, indicating a strong relationship with decreased risk of BCa (P ≤ 0.0001). Furthermore, no association was detected between all the polymorphisms and recurrence-free survival time of overall study population or non-muscle invasive BCa subgroups. In conclusion, rs72552316 in the miRNA binding sites of TLR7 might contribute to BCa susceptibility, and this finding provided new targets for high BCa risk population screening.
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Sihang
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Jiaming
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Periodical Press of West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Urology, West China Hospital, Sichuan University Guoxuexiang 37, Chengdu 610041, Sichuan, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D000368:Aged; D001665:Binding Sites; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D015398:Signal Transduction / Q000235:genetics*; D051193:Toll-Like Receptors / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D051193:Toll-Like Receptors
keywords: Polymorphism; TLRs signaling pathway genes; bladder cancer; microRNA binding sites