title: Growth inhibition and apoptosis induction by Scutellaria pinnatifida A. Ham. on HL-60 and K562 leukemic cell lines.
abstract: A rich flavonoid plant extensively used in traditional medicine is Scutellaria (Lamiaceae). In this study the cytotoxic effects of different extracts of Scutellaria pinnatifida were explored on two different cancer cell lines including K562 and HL-60 and a normal cell line. Also, the role of apoptosis on this toxicity was explored. The cell viability was quantitated by alamarBlue(®) assay. S. pinnatifida could effectively decrease the viability of malignant cells and the CH2Cl2 extract of S. pinnatifida had showed the most cytotoxic effects among other extracts. Apoptosis was confirmed after propidium iodide staining of DNA fragments and detection of the sub-G1 peak in the related flow cytometry histogram of cells. The results of western blot assay showed that CH2Cl2 extract has been able to increase the amount of Bax and cleavage of PARP protein after a 48h contact with cells. Neobaicalein (skullcapﬂavone II) and wogonin were identified in the extract of S. pinnatifida as the active components. The result of the present study confirmed the putative role of neobaicalein (skullcapﬂavone II) and wogonin as cytotoxic components in the CH2Cl2 extract of S. pinnatifida.
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Motahareh
authors.initials: M
authors.lastname: Boozari
authors.affiliation: Research Center of Natural Products Safety and Medicinal Plants, North Khorasan University of Medical Sciences, Bojnurd, 01830-49504, Iran.
authors.forename: Ameneh
authors.initials: A
authors.lastname: Mohammadi
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Asili
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Seyed Ahmad
authors.initials: SA
authors.lastname: Emami
authors.affiliation: Department of Pharmacodynamics and Toxicology, School of Pharmacy; Mashhad University of Medical Sciences, P.O. Box: 9188617871, Mashhad, Iran. Electronic address: tayaraninz@mums.ac.ir.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tayarani-Najaran
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D044950:Flavanones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D007938:Leukemia; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D031363:Scutellaria
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D044950:Flavanones; D005419:Flavonoids; D010936:Plant Extracts; C047405:skullcapflavone II; C085514:wogonin
keywords: Cytotoxicity; Lamiaceae; Neobaicalein; Scutellaria pinnatifida; Wogonin