title: Restorative effect of hair follicular dermal cells on injured human hair follicles in a mouse model.
abstract: No model is available for examining whether in vivo-damaged human hair follicles (hu-HFs) are rescued by transplanting cultured hu-HF dermal cells (dermal papilla and dermal sheath cells). Such a model might be valuable for examining whether in vivo-damaged hu-HFs such as miniaturized hu-HFs in androgenic alopecia are improvable by auto-transplanting hu-HF dermal cells. In this study, we first developed mice with humanized skin composed of hu-keratinocytes and hu-dermal fibroblasts. Then, a 'humanized scalp model mouse' was generated by transplanting hu-scalp HFs into the humanized skin. To demonstrate the usability of the model, the lower halves of the hu-HFs in the model were amputated in situ, and cultured hu-HF dermal cells were injected around the amputated area. The results demonstrated that the transplanted cells contributed to the restoration of the damaged HFs. This model could be used to explore clinically effective technologies for hair restoration therapy by autologous cell transplantation.
authors.affiliation: PhoenixBio Co., Ltd., Higashihiroshima, Hiroshima, Japan.
authors.forename: Mikaru
authors.initials: M
authors.lastname: Yamao
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Inamatsu
authors.forename: Taro
authors.initials: T
authors.lastname: Okada
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishida
authors.forename: Chise
authors.initials: C
authors.lastname: Tateno
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Yoshizato
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000637:transplantation*; D006197:Hair / Q000254:growth & development; D018859:Hair Follicle / Q000166:cytology / Q000293:injuries* / Q000502:physiology* / Q000637:transplantation; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D051379:Mice; D012038:Regeneration; D012535:Scalp; D013997:Time Factors
keywords: cell therapy; dermal papilla cell; dermal sheath cell; hair restoration; humanized scalp model mouse