title: Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.
abstract: OBJECTIVE
  The tumor suppressor gene p53 is mutated in glioblastoma. We studied the relationship between the p53 gene and the biological effects of boron neutron capture therapy (BNCT).
  
  
  METHODS
  The human glioblastoma cells; A172, expressing wild-type p53, and T98G, with mutant p53, were irradiated by the Kyoto University Research Reactor (KUR). The biological effects after neutron irradiation were evaluated by the cell killing effect, 53BP1 foci assay and apoptosis induction.
  
  
  RESULTS
  The survival-fraction data revealed that A172 was more radiosensitive than T98G, but the difference was reduced when boronophenylalanine (BPA) was present. Both cell lines exhibited similar numbers of foci, suggesting that the initial levels of DNA damage did not depend on p53 function. Detection of apoptosis revealed a lower rate of apoptosis in the T98G.
  
  
  CONCLUSIONS
  BNCT causes cell death in glioblastoma cells, regardless of p53 mutation status. In T98G cells, cell killing and apoptosis occurred effectively following BNCT.
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori-cho, Sennann-gun, Osaka, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Seki
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori-cho, Sennann-gun, Osaka, Japan kinashi@rri.kyoto-u.ac.jp.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kinashi
authors.affiliation: Research Reactor Institute, Kyoto University, Kumatori-cho, Sennann-gun, Osaka, Japan.
authors.forename: Sentaro
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D017209:Apoptosis; D016754:Boron Neutron Capture Therapy; D045744:Cell Line, Tumor; D002470:Cell Survival; D005909:Glioblastoma / Q000532:radiotherapy*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D020125:Mutation, Missense; D016159:Tumor Suppressor Protein p53 / Q000502:physiology*; D000071857:Tumor Suppressor p53-Binding Protein 1
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C495901:TP53 protein, human; C498301:TP53BP1 protein, human; D016159:Tumor Suppressor Protein p53; D000071857:Tumor Suppressor p53-Binding Protein 1
keywords: 53BP1; Boron neutron capture therapy; apoptosis; neutron; p53