title: PCL/chitosan/Zn-doped nHA electrospun nanocomposite scaffold promotes adipose derived stem cells adhesion and proliferation.
abstract: Chitosan (Ch), and poly(ɛ-caprolactone) (PCL), widely used as biomaterials with desirable properties for tissue engineering applications, were both blended with zinc-doped hydroxyapatite nanoparticles(nZnHA) and electrospun into nanofibrous scaffolds using formic acid/acetic acid. The rationale behind this study was to demonstrate that presence of small quantities of Zn(2+) ions doped in HA nanoparticles can improve biocompatibility of PCL/Ch blends. SEM observation revealed that average fiber diameter was increased from about 136 nm for a PCL/Ch blend, to around 210 nm for PCL/Ch/nZnHA nanocomposite. PCL/Ch/nZnHA scaffolds offered higher elastic modulus (about 3-fold) and tensile strength (nearly 1.5-fold) than the corresponding PCL/Ch scaffolds. In-vitro biocompatibility studies using human adipose derived stem cells (hAD-MSCs), demonstrated that the presence of only 5 wt% nZnHA in PCL/Ch/nZnHA nanocomposites enhanced hAD-MSCs' attachment compared to PCL/Ch and PCL/Ch/nHA. Finally, hAD-MSCs proliferation occurred at significantly higher rates of 1.5, 1.3 and 1.2 times on PCL/Ch/nZnHA scaffold compared to PCL, PCL/Ch and PCL/Ch/nHA, respectively.
authors.affiliation: Department of Polymer Engineering, University of Tehran, Tehran, Iran. Electronic address: fereshte.may05@gmail.com.
authors.forename: Fereshteh Mohammad
authors.initials: FM
authors.lastname: Ghorbani
authors.affiliation: Department of Polymer Engineering, University of Tehran, Tehran, Iran. Electronic address: kaffashi@ut.ac.ir.
authors.forename: Babak
authors.initials: B
authors.lastname: Kaffashi
authors.affiliation: Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, Iran. Electronic address: p.shokrolahi@ippi.ac.ir.
authors.forename: Parvin
authors.initials: P
authors.lastname: Shokrollahi
authors.affiliation: Department of Biotechnology University of Tehran, Tehran, Iran. Electronic address: seyedjafari@ut.ac.ir.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.affiliation: Stem Cell Technology Research Center, Tehran, Iran. Electronic address: r.ardeshiry.62@gmail.com.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan; D015032:Zinc
keywords: Chitosan; Electrospun scaffold; Polycaprolactone; Tissue engineering; Zn-doped nanohydroxyapatite