title: Alleviative effect of quercetin on rat testis against arsenic: a histological and biochemical study.
abstract: The preventive effect of quercetin on arsenic stimulated reproductive ailments in male Sprague Dawely (SD) rats was investigated. Twenty rats were divided into four groups. The first group served as a control and was provided tap water. The second group of rats was treated with sodium arsenite at the dose of 50 ppm in drinking water. The third group served as a positive control and received an oral dose of quercetin (50 mg/kg). In the fourth group, quercetin (50 mg/kg) was co-administered orally with arsenic (50 ppm in drinking water). All the treatments were carried out for 49 days. Arsenic treatment resulted in adverse morphological and histopathological changes in testis of rats including reduced epithelial height and tubular diameter, and increased luminal diameter. In contrast, these adverse effects of arsenic were eliminated by co-administration of quercetin. Additionally arsenic treatment significantly increased testicular thiobarbituric acid reactive substance (TBARS) levels while catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and glutathione reductase (GSR) activities, and plasma and intra-testicular testosterone concentrations, were decreased significantly. Lipid peroxidation (LPO) was significantly suppressed and depleted antioxidant defense mechanism was restored by the quercetin co-treatment. Also quercetin treatment resulted in a marked increase in plasma and testicular testosterone concentrations. On the basis of these findings, it was concluded that quercetin may be used as a potential therapeutic drug against arsenic induced reproductive toxicity.
authors.affiliation: Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid-i-Azam University , Islamabad , Pakistan and.
authors.forename: Sarwat
authors.initials: S
authors.lastname: Jahan
authors.forename: Natasha
authors.initials: N
authors.lastname: Iftikhar
authors.forename: Hizb
authors.initials: H
authors.lastname: Ullah
authors.forename: Gul
authors.initials: G
authors.lastname: Rukh
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Hussain
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D020261:Arsenic Poisoning / Q000517:prevention & control*; D018053:Arsenites / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017670:Sodium Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D013733:Testicular Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000517:prevention & control*; D013737:Testis / Q000473:pathology; D013739:Testosterone / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018053:Arsenites; D017670:Sodium Compounds; D017392:Thiobarbituric Acid Reactive Substances; D013739:Testosterone; C017947:sodium arsenite; D011794:Quercetin
keywords: Antioxidant enzymes; arsenic; intra-testicular; lipid peroxidation; quercetin