title: A highly sensitive and specific electrochemical sensing method for robust detection of Escherichia coli lac Z gene sequence.
abstract: Based on the interaction mode of capture probe-target-signal probe (CP-T-SP), a simple but efficient electrochemical sensing method is developed for robust detection of Escherichia coli (E. coli) lac Z gene sequence. The sensing system is simply composed of two probes with CP attached to the surface of gold electrode and methylene blue (MB)-labeled SP free in the detecting solution. Two duplexes are formed with the target DNA, an E. coli lac Z gene sequence, hybridizing with two ends of CP and SP separately. This structure can position the MB labels near to the electrode surface, thereby resulting in the change of detecting current. The proposed sensor exhibits a high sensitivity with a detection limit of ∼30 fM for target DNA and good mismatch discrimination ability (MDA). This method can also be applied to the construction of other sensors for the analysis of similar designated pathogenic bacteria gene sequence (PBGS).
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China. Electronic address: yzgyhdzm@126.com.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Qu
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Guiling
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China. Electronic address: yanhong204821@yahoo.com.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.affiliation: Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, Innovative Research Team of Green Chemical Technology in University of Heilongjiang Province (2014TD007), College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Electronic Science and Technology, Harbin University of Science and Technology, Harbin 150080, China.
authors.forename: Xunjun
authors.initials: X
authors.lastname: He
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry; D055664:Electrochemical Techniques; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008751:Methylene Blue; D001616:beta-Galactosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D006046:Gold; D004247:DNA; D001616:beta-Galactosidase; D008751:Methylene Blue
keywords: DNA sensor; Electrochemical sensing method; Escherichia coli lac Z; Methylene blue; Mismatch discrimination