title: Characterization of selenium-enriched mycelia of Catathelasma ventricosum and their antihyperglycemic and antioxidant properties.
abstract: This is the first report concerning the selenium enrichment of Catathelasma ventricosum mycelia. The selenium-containing proteins present in selenium-enriched mycelia (Se-MC) were identified using size-exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS). The selenium-containing amino acids liberated by hydrolysis of these proteins were identified using anion exchange-ICP-MS. Se-MC was found to contain selenoproteins with molecular weights ranging from 1.7 to 60.5 kDa. The main selenium-containing amino acids within them were selenomethionine and selenocysteine. Furthermore, Se-MC possessed excellent antihyperglycemic and antioxidant properties. Se-MC normalized biochemical parameters like insulin level, blood glucose level, body weight, and antioxidant enzyme activity in streptozocin-induced diabetic mice. It also inhibited the α-amylase and α-glucosidase activities present in in vitro gastric and intestinal models. In conclusion, Se-MC has the potential to serve as a dietary supplement of selenium, an antioxidant, or an ingredient for the formulation of nutraceuticals.
authors.affiliation: College of Food Science, Sichuan Agricultural University , Yaan 625014, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Liu
authors.forename: Caiming
authors.initials: C
authors.lastname: Li
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Luo
authors.forename: Guoquan
authors.initials: G
authors.lastname: Han
authors.forename: Shude
authors.initials: S
authors.lastname: Xu
authors.forename: Fuge
authors.initials: F
authors.lastname: Niu
authors.forename: Xinjie
authors.initials: X
authors.lastname: Hu
authors.forename: Hejun
authors.initials: H
authors.lastname: Wu
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry*; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000737:chemistry*; D007328:Insulin / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D025282:Mycelium / Q000737:chemistry; D018036:Selenium Compounds / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D018036:Selenium Compounds
keywords: antihyperglycemic activity; antioxidant activity; chemical composition; mushroom; selenium speciation