title: Overproduction of laccase from a newly isolated Ganoderma lucidum using the municipal food waste as main carbon and nitrogen supplement.
abstract: A strain of Ganoderma lucidum was separated and identified according to its morphological characteristics and phylogenetic data. The fungus is a laccase producer and it can secrete laccase using the municipal food waste (FW) as carbon and nitrogen supplement. After the statistic optimization, a laccase activity of 42,000 ± 600 U/l was obtained at 500 ml flask level and the activity is 12,000 U/l higher than that obtained by fermenting glucose and peptone, indicating that the use of FW to produce laccase not only reduces production cost, but also improves laccase activity. In 15 l bioreactor, FW is also suitable for laccase production and the maximum laccase activity reached 54,000 U/l. Moreover, some details of laccase overproduction using FW were investigated. The G. lucidum consumes FW by secreting a series of hydrolases and proteases and the improvement of laccase activity is because FW induces over-expression of three isoenzymes by polyacrylamide gel electrophoresis analysis.
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang, 453007, China, whl@htu.cn.
authors.forename: Wang
authors.initials: W
authors.lastname: Hailei
authors.forename: Li
authors.initials: L
authors.lastname: Ping
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuhua
authors.forename: Liu
authors.initials: L
authors.lastname: Yufeng
mesh_terms: D000681:Amylases / Q000737:chemistry; D019149:Bioreactors; D002241:Carbohydrates / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005502:Food; D005735:Garbage; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000737:chemistry; D042845:Laccase / Q000096:biosynthesis*; D009584:Nitrogen / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D010802:Phylogeny; D012044:Regression Analysis; D020940:Reishi / Q000201:enzymology*
chemical_list: D002241:Carbohydrates; D002244:Carbon; D042845:Laccase; D006867:Hydrolases; D000681:Amylases; D010447:Peptide Hydrolases; D009584:Nitrogen