title: MicroRNAs in the immune organs of chickens and ducks indicate divergence of immunity against H5N1 avian influenza.
abstract: Chickens are susceptible to the highly pathogenic H5N1 strain of avian influenza virus (HPAIV), whereas ducks are not. Here, we used high-throughput sequencing to analyse the microRNA expression in the spleen, thymus and bursa of Fabricius of H5N1-HPAIV-infected and non-infected chickens and ducks. We annotated the genomic positions of duck microRNAs and we compared the microRNA repertoires of chickens and ducks. Our results showed that the microRNA expression patterns in the homologous immune organs of specific-pathogen-free (SPF) chickens and ducks diverge substantially. Moreover, there was larger divergence between the microRNA expression patterns in immune organs of HPAIV-infected chickens than HPAIV-infected ducks. Together, our results might help to elucidate the roles of microRNAs in the divergent immunity of chickens and ducks against H5N1 HPAIV.
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Zezhong
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Jilin Business and Technology College, Changchun 130507, China.
authors.forename: Jiazi
authors.initials: J
authors.lastname: Su
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Yinuo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Experimental Base of Agriculture, Jilin University, Changchun 130062, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China.
authors.forename: Shenyang
authors.initials: S
authors.lastname: Xing
authors.affiliation: Harbin Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guan
authors.affiliation: Harbin Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Harbin 150001, China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China. Electronic address: sbx@jlu.edu.cn.
authors.forename: Boxing
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Animal Science, Jilin University, Changchun 130062, China; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Center for Animal Embryo Engineering of Jilin Province, Jilin University, Changchun 130062, China. Electronic address: zhzhao@jlu.edu.cn.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002060:Bursa of Fabricius / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002645:Chickens / Q000276:immunology / Q000378:metabolism / Q000821:virology; D004372:Ducks / Q000276:immunology / Q000378:metabolism / Q000821:virology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D005585:Influenza in Birds / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000821:virology; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism / Q000821:virology; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Chicken; Duck; Highly pathogenic H5N1 avian influenza; Immune organ; MicroRNA