title: Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection.
abstract: Half-smooth tongue sole (Cynoglossus semilaevis) is one of the most valuable marine aquatic species in Northern China. Given to the rapid development of aquaculture industry, the C. semilaevis was subjected to disease-causing bacteria Vibrio anguillarum. It therefore is indispensable and urgent to understand the mechanism of C. semilaevis host defense against V. anguillarum infection. In the present study, the extensively analysis at the transcriptome level for V. Anguillarum disease in tongue sole was carried out. In total, 94,716 high quality contigs were generated from 75,884,572 clean reads in three libraries (HOSG, NOSG, and CG). 22,746 unigenes were identified when compared with SwissProt, an NR protein database and NT nucleotide database. 954 genes exhibiting the differentially expression at least one pair of comparison in all three libraries were identified. GO enrichment for these genes revealed gene response to biotic stimulus, immune system regulation, and immune response and cytokine production. Further, the pathways such as complement and coagulation cascades and Vibrio cholerae infection pathways were enriched in defensing of pathogen. Besides, 13,428 SSRs and 118,239 SNPs were detected in tongue sole, providing further support for genetic variation and marker-assisted selection in future. In summary, this study identifies several putative immune pathways and candidate genes deserving further investigation in the context of development of therapeutic regimens and lays the foundation for selecting resistant lines of C. semilaevis against V. anguillarum.
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China; Laboratory of Marine Biodiversity and Global Change, College of Oceanography and Environmental Science, Xiamen University, 182 Daxue Road, Xiamen 361005, Fujian, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Psychiatry & Neurobiology Science, University of Virginia, 1670 Discovery Drive, Suite 110, Charlottesville 22911, VA, USA.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Songlin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Changwei
authors.initials: C
authors.lastname: Shao
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Qilong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China.
authors.forename: Guangye
authors.initials: G
authors.lastname: Gong
authors.affiliation: Laboratory of Marine Biodiversity and Global Change, College of Oceanography and Environmental Science, Xiamen University, 182 Daxue Road, Xiamen 361005, Fujian, China.
authors.forename: Shaoxiong
authors.initials: S
authors.lastname: Ding
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, Shandong, China. Electronic address: shazx@ysfri.ac.cn.
authors.forename: Zhenxia
authors.initials: Z
authors.lastname: Sha
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes; D020869:Gene Expression Profiling / Q000662:veterinary; D015723:Gene Library; D018895:Microsatellite Repeats; D009928:Organ Specificity; D020641:Polymorphism, Single Nucleotide; D059467:Transcriptome; D014733:Vibrio / Q000502:physiology*; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000662:veterinary*
chemical_list: D029941:Fish Proteins
keywords: Cynoglossus semilaevis; High-throughput sequencing; Immune; Transcriptome; Vibrio anguillarum