title: Involvement of pituitary tumor transforming gene 1 in psoriasis, seborrheic keratosis, and skin tumors.
abstract: Accumulating evidence suggests that pituitary tumor transforming gene 1 (PTTG1) is a potential biomarker for cancer malignancy and a cell-cycle regulatory protein. This investigation was performed to address the subcellular localization of PTTG1 and its possible involvement in proliferative skin diseases. In vitro primary-cultured keratinocytes and skin samples from psoriasis, seborrheic keratosis (SK), basal cell carcinoma (BCC), and squamous cell carcinoma (SCC) were investigated by immunofluorescence and real-time PCR. In normal skin, PTTG1 is localized predominantly in 10% of basal keratinocytes, while 30-40% in basal and suprabasal psoriatic keratinocytes. PTTG1 mRNA in psoriatic epidermis is about 5-fold more than that in normal one (P<0.01). PTTG1 is localized in cytoplasm in primary-cultured normal and psoriatic keratinocytes, and PTTG1 in HaCaT cells is distributed throughout the cytoplasm of metaphase cells. PTTG1 is seen at both G2 and M phases, and highest PTTG1 expression correlates with highest cyclin B1 expression and highest degree of nuclear pleomorphism at M phase. The positive rate of PTTG1 in SK, BCC, and SCC is about 10%, 20%, and more than 80%, respectively. PTTG1 siRNA, which knocks down the expression of PTTG1, reduced the invasive capacity of A431 cells. In conclusion, PTTG1 is a marker for proliferative skin diseases associated with cell cycle regulation and may aid in detection of aggressive cancers.
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Sui-Qing
authors.initials: SQ
authors.lastname: Cai
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Dou
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Rehabilitation, Qianfoshan Hospital, Shandong University, Jinan, Shandong, 250014, China.
authors.forename: Shu-Qing
authors.initials: SQ
authors.lastname: Li
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Department of Dermatology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310009, China.
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Man
mesh_terms: D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism*; D016022:Case-Control Studies; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D055785:Gene Knockdown Techniques; D006801:Humans; D017492:Keratosis, Seborrheic / Q000378:metabolism* / Q000473:pathology; D009361:Neoplasm Invasiveness; D011565:Psoriasis / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D064249:Securin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human