title: MLST-based inference of genetic diversity and population structure of clinical Klebsiella pneumoniae, China.
abstract: Multilocus sequence typing was applied to a collection of 327 clinical isolates of Klebsiella pneumoniae from China, which was proven to be a good representative of the global diversity of K. pneumoniae. Three lineages L1 to L3 are presented in the population with limited genetic flow across different lineages. However, extremely high levels of recombination can be observed within lineages to the extent at which the alleles are associated almost randomly. Lineages L2 and L3 most likely represent highly specific subgroups of less-virulent K. pneumoniae with modified metabolic networks, while lineage L1 contains not only hypervirulent clones with massive acquisition of virulent genes but also 'primitive and intermediate forms' during evolution of hypervirulent K. pneumoniae.
authors.affiliation: Consulting Center of Biomedical Statistics, Beijing 100850, China.
authors.forename: Chenyi
authors.initials: C
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Xianwei
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Yarong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Han
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Consulting Center of Biomedical Statistics, Beijing 100850, China.
authors.forename: Liangping
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics; D002681:China; D018389:Codon, Nonsense; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D005798:Genes, Bacterial; D008040:Genetic Linkage; D056426:Genetic Loci; D014644:Genetic Variation; D005838:Genotype; D019746:Intramolecular Oxidoreductases / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D001426:Bacterial Proteins; D018389:Codon, Nonsense; D003577:Cytochrome P-450 Enzyme System; D019746:Intramolecular Oxidoreductases; C022461:prostacyclin synthetase