title: Phage selection assisted by Sfp phosphopantetheinyl transferase-catalyzed site-specific protein labeling.
abstract: Phosphopantetheinyl transferases (PPTase) Sfp and AcpS catalyze a highly efficient reaction that conjugates chemical probes of diverse structures to proteins. PPTases have been widely used for site-specific protein labeling and live cell imaging of the target proteins. Here we describe the use of PPTase-catalyzed protein labeling in protein engineering by facilitating high-throughput phage selection.
authors.affiliation: Department of Chemistry, University of Chicago, 929 E57th Street, Chicago, IL, 60637, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhao
authors.forename: Keya
authors.initials: K
authors.lastname: Zhang
authors.forename: Karan
authors.initials: K
authors.lastname: Bhuripanyo
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Wang
authors.forename: Han
authors.initials: H
authors.lastname: Zhou
authors.forename: Mengnan
authors.initials: M
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D004926:Escherichia coli; D019151:Peptide Library; D015202:Protein Engineering; D013194:Staining and Labeling; D019809:Streptavidin / Q000737:chemistry; D017855:Transferases (Other Substituted Phosphate Groups) / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D019151:Peptide Library; C082662:phosphopantetheinyl transferase; D001710:Biotin; D019809:Streptavidin; D017855:Transferases (Other Substituted Phosphate Groups)