title: Expression levels of microRNA-199 and hypoxia-inducible factor-1 alpha in brain tissue of patients with intractable epilepsy.
abstract: OBJECTIVE
  During the last decade, experimental evidence has demonstrated an important role of hypoxia, which leads to neuronal cell death and angiogenesis, in the mechanisms of seizure precipitation and recurrence. MicroRNA-199 targets hypoxia-inducible factor-1alpha (HIF-1α), which has recently been implicated in the pathophysiology of the hypoxic state and brain injury. However, little is known about the roles of MicroRNA-199 and HIF-1α in the human epileptogenic process.
  
  
  METHODS
  In this study, we investigated the expression of miR-199a-5p, miR-199b-5p and HIF-1α using real-time PCR, immunohistochemistry and western blots in the temporal neocortex of twenty four patients with intractable epilepsy and twelve control subjects.
  
  
  RESULTS
  Compared with the control group, the expression of miR-199a-5p and miR-199b-5p was significantly lower in epileptic brain tissues (p < 0.05). The levels of HIF-1α mRNA and protein were highly up-regulated in epileptic brain tissues compared with those of control subjects (p < 0.05).
  
  
  CONCLUSIONS
  These data suggest that the abnormal expression of miR-199 and HIF-1α in epileptic brain tissue may be involved in the pathophysiology of human epilepsy and that the expression of HIF-1α may be regulated by miR-199. These findings may provide new insights into the treatment of epilepsy.
authors.affiliation: a 1 Department of Neurology, Institute of Neurology, Affiliated Hospital of North Sichuan Medical College , Wen Hua Road, Nanchong 637000 , China.
authors.forename: Guohui
authors.initials: G
authors.lastname: Jiang
authors.affiliation: a 1 Department of Neurology, Institute of Neurology, Affiliated Hospital of North Sichuan Medical College , Wen Hua Road, Nanchong 637000 , China.
authors.forename: Ruijiao
authors.initials: R
authors.lastname: Zhou
authors.affiliation: b 2 Department of Neurosurgery, Daping Hospital and Institute of Surgery Research, Third Military Medical University , Chongqing 400042 , China.
authors.forename: Xuzhi
authors.initials: X
authors.lastname: He
authors.affiliation: c 3 Diagnosis Department of Hebei Medical University , Shijiazhuang 050000 , China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Shi
authors.affiliation: a 1 Department of Neurology, Institute of Neurology, Affiliated Hospital of North Sichuan Medical College , Wen Hua Road, Nanchong 637000 , China.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: a 1 Department of Neurology, Institute of Neurology, Affiliated Hospital of North Sichuan Medical College , Wen Hua Road, Nanchong 637000 , China.
authors.forename: Juming
authors.initials: J
authors.lastname: Yu
authors.affiliation: a 1 Department of Neurology, Institute of Neurology, Affiliated Hospital of North Sichuan Medical College , Wen Hua Road, Nanchong 637000 , China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
mesh_terms: D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D000069279:Drug Resistant Epilepsy / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D013702:Temporal Lobe / Q000378:metabolism*; D055815:Young Adult
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D035683:MicroRNAs; C531408:mirn199 microRNA, human
keywords: epilepsy; hypoxia; hypoxia-inducible factor-1α; miRNA-199; recurrent seizures