title: Determination of beta-agonists in swine hair by μFIA and chemiluminescence.
abstract: β-Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the β-agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the β-agonists. The chip was fabricated from two glass plates (64 mm × 32 mm) with microchannels of 200 μm width and 100 μm depth. The detection limits were achieved at 2.0 × 10(-8) mol/L of ractopamine, 1.0 × 10(-8) mol/L of terbutaline and 5.0 × 10(-7) mol/L of salbutamol. In this report, our method was applied for determination of the β-agonists in swine hair from three different sources with satisfactory results.
authors.affiliation: Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; School of Pharmaceutical Science and Technology and State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: Shi
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Gao
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.forename: Xianming
authors.initials: X
authors.lastname: Liu
authors.forename: Weijie
authors.initials: W
authors.lastname: Zhao
authors.forename: Bingcheng
authors.initials: B
authors.lastname: Lin
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry; D000420:Albuterol / Q000032:analysis; D000818:Animals; D004867:Equipment Design; D005292:Ferricyanides / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation / Q000379:methods; D006197:Hair / Q000737:chemistry*; D007700:Kinetics; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D010627:Phenethylamines / Q000032:analysis; D034421:Sus scrofa; D013726:Terbutaline / Q000032:analysis
chemical_list: D000318:Adrenergic beta-Agonists; D005292:Ferricyanides; D010627:Phenethylamines; C060897:ractopamine; D008165:Luminol; D013726:Terbutaline; D000420:Albuterol; C028033:potassium ferricyanide
keywords: Beta-agonists; Chemiluminescence; Microflow injection analysis; Microfluidic chip