title: Identification of genes associated with reproduction in the Mud Crab (Scylla olivacea) and their differential expression following serotonin stimulation.
abstract: The central nervous system (CNS) is often intimately involved in reproduction control and is therefore a target organ for transcriptomic investigations to identify reproduction-associated genes. In this study, 454 transcriptome sequencing was performed on pooled brain and ventral nerve cord of the female mud crab (Scylla olivacea) following serotonin injection (5 µg/g BW). A total of 197,468 sequence reads was obtained with an average length of 828 bp. Approximately 38.7% of 2,183 isotigs matched with significant similarity (E value < 1e-4) to sequences within the Genbank non-redundant (nr) database, with most significant matches being to crustacean and insect sequences. Approximately 32 putative neuropeptide genes were identified from nonmatching blast sequences. In addition, we identified full-length transcripts for crustacean reproductive-related genes, namely farnesoic acid o-methyltransferase (FAMeT), estrogen sulfotransferase (ESULT) and prostaglandin F synthase (PGFS). Following serotonin injection, which would normally initiate reproductive processes, we found up-regulation of FAMeT, ESULT and PGFS expression in the female CNS and ovary. Our data here provides an invaluable new resource for understanding the molecular role of the CNS on reproduction in S. olivacea.
authors.affiliation: Chulabhorn International College of Medicine, Thammasat University, Pathumthani, 12121, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Napamanee
authors.initials: N
authors.lastname: Kornthong
authors.affiliation: Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, 4558, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan, 60130, Thailand.
authors.forename: Charoonroj
authors.initials: C
authors.lastname: Chotwiwatthanakun
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Kanjana
authors.initials: K
authors.lastname: Khornchatri
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Attakorn
authors.initials: A
authors.lastname: Engsusophon
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand; Pro Vice-Chancellor's Office, Faculty of Science, Engineering and Built Environment, Deakin University, Locked Bag 20000, Geelong, Victoria, 3220, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hanna
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000502:physiology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D005260:Female; D063990:Gene Ontology; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics; D010053:Ovary / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000187:drug effects / Q000235:genetics; D017421:Sequence Analysis; D012701:Serotonin / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D009479:Neuropeptides; D012333:RNA, Messenger; D012701:Serotonin