title: Expression of tumor necrosis factor-alpha-mediated genes predicts recurrence-free survival in lung cancer.
abstract: In this study, we conducted a meta-analysis on high-throughput gene expression data to identify TNF-α-mediated genes implicated in lung cancer. We first investigated the gene expression profiles of two independent TNF-α/TNFR KO murine models. The EGF receptor signaling pathway was the top pathway associated with genes mediated by TNF-α. After matching the TNF-α-mediated mouse genes to their human orthologs, we compared the expression patterns of the TNF-α-mediated genes in normal and tumor lung tissues obtained from humans. Based on the TNF-α-mediated genes that were dysregulated in lung tumors, we developed a prognostic gene signature that effectively predicted recurrence-free survival in lung cancer in two validation cohorts. Resampling tests suggested that the prognostic power of the gene signature was not by chance, and multivariate analysis suggested that this gene signature was independent of the traditional clinical factors and enhanced the identification of lung cancer patients at greater risk for recurrence.
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Baohua
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Respiratory Medicine, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Song
authors.affiliation: Nanjing Decode Genomics Biotechnology Co., Ltd., Nanjing, 210019, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Lianya
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Helin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Respiratory Medicine, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Duan
authors.affiliation: Department of Respiratory Medicine, The Second Hospital of HeBei Medical University, Shijiazhuang, 050000, China.
authors.forename: Wenshu
authors.initials: W
authors.lastname: He
authors.affiliation: School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, 210096, China; College of Information Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yihua
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, 210096, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Nanjing Decode Genomics Biotechnology Co., Ltd., Nanjing, 210019, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhu
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics; D015999:Multivariate Analysis; D009364:Neoplasm Recurrence, Local / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012008:Recurrence; D018570:Risk Assessment; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha