title: Input- and cell-type-specific endocannabinoid-dependent LTD in the striatum.
abstract: Changes in basal ganglia plasticity at the corticostriatal and thalamostriatal levels are required for motor learning. Endocannabinoid-dependent long-term depression (eCB-LTD) is known to be a dominant form of synaptic plasticity expressed at these glutamatergic inputs; however, whether eCB-LTD can be induced at all inputs on all striatal neurons is still debatable. Using region-specific Cre mouse lines combined with optogenetic techniques, we directly investigated and distinguished between corticostriatal and thalamostriatal projections. We found that eCB-LTD was successfully induced at corticostriatal synapses, independent of postsynaptic striatal spiny projection neuron (SPN) subtype. Conversely, eCB-LTD was only nominally present at thalamostriatal synapses. This dichotomy was attributable to the minimal expression of cannabinoid type 1 (CB1) receptors on thalamostriatal terminals. Furthermore, coactivation of dopamine receptors on SPNs during LTD induction re-established SPN-subtype-dependent eCB-LTD. Altogether, our findings lay the groundwork for understanding corticostriatal and thalamostriatal synaptic plasticity and for striatal eCB-LTD in motor learning.
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Jae-Ick
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Vivianne L
authors.initials: VL
authors.lastname: Tawfik
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Rupa R
authors.initials: RR
authors.lastname: Lalchandani
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Palo Alto, CA 94304, USA; Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Palo Alto, CA 94304, USA.
authors.forename: Grégory
authors.initials: G
authors.lastname: Scherrer
authors.affiliation: Department of Neurosurgery, Stanford University School of Medicine, Palo Alto, CA 94304, USA; Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Palo Alto, CA 94304, USA. Electronic address: dingjun@stanford.edu.
authors.forename: Jun B
authors.initials: JB
authors.lastname: Ding
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D003342:Corpus Striatum / Q000378:metabolism / Q000473:pathology; D063388:Endocannabinoids / Q000378:metabolism*; D015870:Gene Expression; D007858:Learning / Q000502:physiology; D036881:Long-Term Synaptic Depression; D051379:Mice; D009046:Motor Neurons / Q000378:metabolism / Q000473:pathology; D009473:Neuronal Plasticity; D043884:Receptor, Cannabinoid, CB1 / Q000096:biosynthesis* / Q000378:metabolism; D013569:Synapses / Q000378:metabolism / Q000473:pathology; D013788:Thalamus / Q000378:metabolism / Q000473:pathology
chemical_list: D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1