title: [The serumproteomics research of vinyl chloride workers].
abstract: OBJECTIVE
  To screen out serum differential proteins between vinyl chloride monomer (VCM)-exposed workers and healthy controls by proteomics and analyze the functions of differential proteins, and to provide a basis for elucidating the pathogenesis of diseases caused by VCM exposure and searching for the protein biomarkers.
  
  
  METHODS
  Fasting venous blood was collected from 125 VCM-exposed workers and 40 healthy controls according to accumulated exposure doses. Proteins were precipitated by acetone precipitation. These proteins were identified by 2D-nano LC-ESI-TOF/MS and quantified by isobaric tags for relative and absolute quantitation. The functions of differential proteins were analyzed by gene ontology.
  
  
  RESULTS
  A total of 596 proteins were identified, including 194 quantified proteins. There were 21 differential proteins according to the screening criteria (19 upregulated proteins and 2 downregulated proteins), including complement, apolipoprotein, and glycoprotein. The functions of these differential proteins were binding, enzyme regulator activity, catalytic activity, and transporter activity, and they were involved in the biological processes including immune system process and response to stimulus.
  
  
  CONCLUSIONS
  The complement, apolipoprotein, and glycoprotein identified in the proteomics may be related to liver injury caused by VCM exposure, and they could be used as candidate protein biomarkers of diseases caused by VCM exposure.
authors.affiliation: Binhai New Area Tanggu Jiefang Street Community Health Service Center, Tianjin 300450, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Jiyan
authors.initials: J
authors.lastname: Gao
authors.forename: Shuifu
authors.initials: S
authors.lastname: Ruan
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: E-mail: tangnaijun@tijmu.edu.cn.
authors.forename: Naijun
authors.initials: N
authors.lastname: Tang
authors.forename: Jingliang
authors.initials: J
authors.lastname: Liang
authors.forename: Jinrong
authors.initials: J
authors.lastname: Liu
mesh_terms: D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000293:injuries; D016273:Occupational Exposure; D011506:Proteins / Q000378:metabolism; D040901:Proteomics; D014752:Vinyl Chloride / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D001798:Blood Proteins; D011506:Proteins; D014752:Vinyl Chloride