title: Oxygen-glucose deprivation inducing B1 RNA inhibits neuronal cells metabolic activity by NLRP3 and associated proinflammatory cytokines production.
abstract: Cerebral ischemia occurs when blood flow to part of the brain is obstructed, which can result in oxygen and glucose deprivation (OGD) and neuronal damage. However, the mechanisms remain poorly understood. The present study investigated the production and effects of double-stranded RNA (dsRNA) induced by OGD in neuronal cells. By confocal microscopy, dsRNA containing B1 and B2 RNA, was found accumulating in HT22 cells under OGD treatment. The sequence of B1 RNA was identified and transfected into HT22 cells. Interestingly, B1 RNA induced transcription and expression of NLRP3, interleukin (IL)-1β, and tumor necrosis factor (TNF)-α, which was similar to the effects of OGD treatment. Moreover, HT22 cell growth inhibition and proinflammatory cytokines production induced by OGD and B1 RNA treatment were down-regulated by NLRP3 knock-down. These findings suggest that B1 RNA induced by OGD forms as dsRNA and inhibits neuronal cell metabolic activity by regulating the NLRP3 and associated proinflammatory cytokines production.
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an 710032, PR China; Department of Pharmacogenomics, School of Pharmacy, The Fourth Military Medical University,Xi'an 710032, PR China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an 710032, PR China; Department of Pharmacogenomics, School of Pharmacy, The Fourth Military Medical University,Xi'an 710032, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Hui
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Rao
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Peng
authors.affiliation: Department of Surgery, Boston Veterans Affairs Healthcare System, Boston University School of Medicine, Boston, MA 02130, USA.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an 710032, PR China; Department of Pharmacogenomics, School of Pharmacy, The Fourth Military Medical University,Xi'an 710032, PR China. Electronic address: yanzhen@fmmu.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, PR China. Electronic address: feizhou@fmmu.edu.cn.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fei
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005947:Glucose / Q000172:deficiency*; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D008807:Mice, Inbred BALB C; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D009474:Neurons / Q000473:pathology; D010100:Oxygen / Q000378:metabolism*; D010957:Plasmids; D012313:RNA / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*; D018626:Retroelements; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002352:Carrier Proteins; D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D012330:RNA, Double-Stranded; D018626:Retroelements; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D005947:Glucose; D010100:Oxygen
keywords: B1 RNA; Cerebral ischemia; NLRP3; Oxygen and glucose deprivation; dsRNA