title: Diagnosis of cutaneous leishmaniasis: why punch when you can scrape?
abstract: Cutaneous leishmaniasis (CL) has been introduced to the Leishmania under-endemic Lebanese population in an uncontrolled manner as a result of recent large-scale displacement of refugees from endemic Syria. Accordingly, a quick and reliable method to diagnose CL is essential. Matched punch biopsies and air-dried scrapings on 72 patients were obtained. Scrapings were collected in two forms: thick drop (N = 33) or thin smear (N = 39). Clinical information was recorded. Sections of punch biopsies and scrapings were stained and examined microscopically. Polymerase chain reaction (PCR) was performed on both scraping forms and biopsies. The diagnostic sensitivity of the tests performed revealed that microscopy in conjunction with PCR on punch biopsies was the most sensitive test (93%) overall. However, taken individually, microscopy and PCR yielded the highest sensitivities when performed on drop scrapings (63% and 85%, respectively), and not smear scrapings (38% and 56%, respectively) as compared with the punch biopsies (44% and 83%, respectively). Microscopic concordance for punch biopsies and drop scrapings was present in 25 of 33 cases. Concordance was predicted only by the high/low parasitic index (PI: 3.1 ± 1.7 and 0.4 ± 0.5, respectively; P < 0.05). Herein, we optimized a novel rapid method for reliable diagnosis of CL based on drop scrapings with good agreement with the gold standard punch biopsy technique.
authors.affiliation: Department of Pathology and Laboratory Medicine, Department of Internal Medicine, Outcomes Research Unit, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Mario
authors.initials: M
authors.lastname: Saab
authors.affiliation: Department of Pathology and Laboratory Medicine, Department of Internal Medicine, Outcomes Research Unit, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Hussein
authors.initials: H
authors.lastname: El Hage
authors.affiliation: Department of Pathology and Laboratory Medicine, Department of Internal Medicine, Outcomes Research Unit, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Khalil
authors.initials: K
authors.lastname: Charafeddine
authors.affiliation: Department of Pathology and Laboratory Medicine, Department of Internal Medicine, Outcomes Research Unit, American University of Beirut Medical Center, Beirut, Lebanon.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Habib
authors.affiliation: Department of Pathology and Laboratory Medicine, Department of Internal Medicine, Outcomes Research Unit, American University of Beirut Medical Center, Beirut, Lebanon ik08@aub.edu.lb.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Khalifeh
mesh_terms: D000293:Adolescent; D000328:Adult; D001707:Biopsy, Needle; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007891:Leishmania / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology*; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012867:Skin; D012882:Skin Tests / Q000379:methods; D055815:Young Adult