title: Fatty acid binding protein FABP3 from Chinese mitten crab Eriocheir sinensis participates in antimicrobial responses.
abstract: Intracellular fatty acid-binding proteins (FABPs) are members of the lipid-binding protein superfamily. Aside from the main functions of FABPs in the uptake and transport of fatty acids, they are also critical in innate immunology. In this work, the full-length cDNA for a Chinese mitten crab Eriocheir sinensis FABP (Es-FABP3) was cloned with an open reading frame of 402 bp encoding a 133 amino acid polypeptide. Analysis using quantitative real-time PCR (qPCR) revealed that Es-FABP3 transcripts were widely distributed in gills, muscle, intestine, hepatopancreas, eyestalk, heart, stomach, brain, thoracic ganglia and hemocytes. After challenge with pathogen associated molecular pattern molecules (PAMPs), the relative mRNA expression levels of Es-FABP3 increased in hepatopancreas, gills and hemocytes. Moreover, the mature recombinant Es-FABP3 protein exhibited different binding activities to bacteria and fungus and inhibited the growth of different microbes. These collective results demonstrated the role of Es-FABP3 in the immunoreactions of E. sinensis to PAMPs.
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Shang-Jian
authors.initials: SJ
authors.lastname: Tan
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Xing-Kun
authors.initials: XK
authors.lastname: Jin
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Li
authors.affiliation: Aquatic Animal Breeding Center of Shanghai University Knowledge Service Platform, Shanghai Ocean University, Shanghai, China. Electronic address: jy-li@shou.edu.cn.
authors.forename: Jia-Yao
authors.initials: JY
authors.lastname: Li
authors.affiliation: School of Life Science, East China Normal University, Shanghai, China. Electronic address: qwang@bio.ecnu.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000502:physiology; D006094:Gram-Positive Bacteria / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D010843:Pichia / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D000890:Anti-Infective Agents; D060829:Arthropod Proteins; D018076:DNA, Complementary; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger
keywords: Eriocheir sinensis; FABP; Immunity; PAMPs