title: Resistance Exercise Training Alters Mitochondrial Function in Human Skeletal Muscle.
abstract: BACKGROUND
  Loss of mitochondrial competency is associated with several chronic illnesses. Therefore, strategies that maintain or increase mitochondrial function will likely be of benefit in numerous clinical settings. Endurance exercise has long been known to increase mitochondrial function in the skeletal muscle. Comparatively little is known regarding the effect of resistance exercise training (RET) on skeletal muscle mitochondrial respiratory function.
  
  
  OBJECTIVE
  The purpose of the current study was to determine the effect of chronic resistance training on skeletal muscle mitochondrial respiratory capacity and function.
  
  
  METHODS
  Here, we studied the effect of a 12-wk RET program on skeletal muscle mitochondrial function in 11 young healthy men. Muscle biopsies were collected before and after the 12-wk training program, and mitochondrial respiratory capacity was determined in permeabilized myofibers by high-resolution respirometry.
  
  
  RESULTS
  RET increased lean body mass and quadriceps muscle strength by 4% and 15%, respectively (P < 0.001). Coupled mitochondrial respiration supported by complex I, and complex I and II substrates increased by 2- and 1.4-fold, respectively (P < 0.01). The ratio of coupled complex I-supported respiration to maximal respiration increased with RET (P < 0.05), as did complex I protein abundance (P < 0.05), whereas the substrate control ratio for succinate was reduced after RET (P < 0.001). Transcripts responsible for proteins critical to electron transfer and NAD production increased with training (P < 0.05), whereas transcripts involved in mitochondrial biogenesis were unaltered.
  
  
  CONCLUSIONS
  Collectively, 12 wk of RET resulted in qualitative and quantitative changes in skeletal muscle mitochondrial respiration. This adaptation was accompanied by modest changes in mitochondrial proteins and transcript expression. RET seems to be a means to augment the respiratory capacity and intrinsic function of skeletal muscle mitochondria.
authors.affiliation: 1Metabolism Unit, Shriners Hospitals for Children, Galveston, TX; 2Department of Surgery, University of Texas Medical Branch, Galveston, TX; 3Rehabilitation Sciences, University of Texas Medical Branch, Galveston, TX; 4Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX; and 5Department of Nutrition and Metabolism, University of Texas Medical Branch, Galveston, TX.
authors.forename: Craig
authors.initials: C
authors.lastname: Porter
authors.forename: Paul T
authors.initials: PT
authors.lastname: Reidy
authors.forename: Nisha
authors.initials: N
authors.lastname: Bhattarai
authors.forename: Labros S
authors.initials: LS
authors.lastname: Sidossis
authors.forename: Blake B
authors.initials: BB
authors.lastname: Rasmussen
mesh_terms: D000328:Adult; D015992:Body Mass Index; D019069:Cell Respiration; D045222:Electron Transport Chain Complex Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008931:Mitochondria, Muscle / Q000378:metabolism*; D053580:Muscle Strength / Q000502:physiology; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000378:metabolism*; D010806:Physical Education and Training / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D055070:Resistance Training / Q000379:methods*; D055815:Young Adult
chemical_list: D045222:Electron Transport Chain Complex Proteins; D012333:RNA, Messenger