title: [Clinical value of flow cytometry in assessing bone marrow involvement of non-Hodgkin's lymphoma].
abstract: OBJECTIVE
  To evaluate the value of flow cytometry (FCM) in the diagnosis of bone marrow involvement in patients with non-Hodgkin's lymphoma (NHL).
  
  
  METHODS
  The cytomorphology, biopsy and FCM tests were performed concurrently on bone marrow samples from 206 patients who were consecutively diagnosed as NHL from March 2013 to August 2013 at Peking University Cancer Hospital. The results were analyzed and compared.
  
  
  RESULTS
  Totally bone marrow involvement occurred in 25.7% (53/206) patients. The detection positivity of bone marrow involvement by cytomorphology, biopsy and flow cytometry were 6/14, 6/14 and 9/14 respectively in 14 T-cell lymphoblastic lymphoma and T-cell acute lymphoblastic leukemia (T-LBL/ALL) patients; 9.2% (14/152) , 19.1% (29/152) and 23.7% (36/152) respectively in 152 mature B-cell NHL patients; 0 (0/40) , 15.0% (6/40) and 2.5% (1/40) in 40 cases of mature T/NK-cell NHL. The overall rate of concordance of biopsy and flow cytometry was 88.3%.
  
  
  CONCLUSIONS
  FCM has a high sensitivity in detecting bone marrow involvement in NHL patients, especially those with T-LBL/ALL and B-cell NHL. And a combination of morphology, biopsy and FCM may improve the sensitivity and accuracy in the detection of bone marrow involvement in NHL patients.
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Song
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Deng
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Xianghong
authors.initials: X
authors.lastname: Li
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Xiaopei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Ningjing
authors.initials: N
authors.lastname: Lin
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Meifeng
authors.initials: M
authors.lastname: Tu
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Zhitao
authors.initials: Z
authors.lastname: Ying
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Lingyan
authors.initials: L
authors.lastname: Ping
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Liu
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Peking University Cancer Hospital and Institute, Department of Lymphoma, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Beijing 100142, China. Email: zhujun@csco.org.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
mesh_terms: D001402:B-Lymphocytes; D001706:Biopsy; D001853:Bone Marrow; D005434:Flow Cytometry; D006801:Humans; D008228:Lymphoma, Non-Hodgkin; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; D013601:T-Lymphocytes