title: Epstein-Barr virus infection is inversely correlated with the expression of retinoblastoma protein in Reed-Sternberg cells in classic Hodgkin lymphoma.
abstract: Classic Hodgkin lymphoma (cHL) is characterized by few neoplastic Hodgkin/Reed-Sternberg (H/RS) cells in a background of intense inflammatory infiltrate. Epstein-Barr virus (EBV) has been shown to affect cell cycle and regulation of apoptosis. In total, 82 cases of cHL were studied. Five- micrometer sections were prepared and stained with haematoxylin and eosin and immunohistochemical streptavidin-biotin methods for EBV-LMP-1, pRb, ki-67 and cleaved caspase-3. In-situ hybridization for EBV encoded RNA was used to confirm the detection of EBV in H/RS cells. There were 45 nodular sclerosis, 28 mixed cellularity, 4 lymphocyte-rich, and 5 lymphocyte depletion subtypes in this series of cases. EBV and pRb were detected in 55% (46/82) and 64% (50/82) of the cases respectively. EBV was detected in 78% (25/32) of pRb-negative cases and 81% (29/36) of EBV-negative cases are pRb-positive. A statistically significant inverse relationship was observed between the presence of EBV and expression of pRb (P = 0.001). In conclusion, EBV infection is inversely correlated with pRb in H/RS cells in cHL.
authors.affiliation: Department of Pathology, College of Medicine and Health Sciences, United Arab Emirates University Al Ain, United Arab Emirates.
authors.forename: Suhail
authors.initials: S
authors.lastname: Al-Salam
authors.affiliation: Department of Laboratory Medicine, Tawam Hospital in Affiliation with Johns Hopkins International Medicine Al Ain, United Arab Emirates.
authors.forename: Aktham
authors.initials: A
authors.lastname: Awwad
authors.affiliation: Department Of Pathology, University of Utah, Primary Children's Hospital 100 North Mario Capecchi Drive, Salt Lake City, UT 84103, USA.
authors.forename: Mouied
authors.initials: M
authors.lastname: Alashari
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D002455:Cell Division; D049109:Cell Proliferation; D020031:Epstein-Barr Virus Infections / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006689:Hodgkin Disease / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D006801:Humans; D017403:In Situ Hybridization; D019394:Ki-67 Antigen / Q000378:metabolism; D008214:Lymphocytes / Q000473:pathology; D012367:RNA, Viral / Q000235:genetics; D016539:Reed-Sternberg Cells / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D016160:Retinoblastoma Protein / Q000378:metabolism*
chemical_list: D019394:Ki-67 Antigen; D012367:RNA, Viral; D016160:Retinoblastoma Protein; D053148:Caspase 3
keywords: Epstein Barr virus; Hodgkin lymphoma; pRb