title: Utility of RAPD marker for genetic diversity analysis in gamma rays and ethyl methane sulphonate (EMS)-treated Jatropha curcas plants.
abstract: The presence of important chemical and physical properties in Jatropha curcas makes it a valuable raw material for numerous industrial applications, including the production of biofuel. Hence, the researcher's interest is diversified to develop more and better varieties with outstanding agronomic characteristics using conventional breeding. Among these, mutation breeding is one of the best approaches to bring genetic changes in plant species. The aim of this study is to evaluate the diversity and genetic relationship among J. curcas mutants, which were obtained from different doses of gamma rays (control, 5 Kr, 10 Kr, 15 Kr, 20 Kr and 25 Kr) and EMS (1%, 2%, 3% and 4%), using RAPD marker. Among the 21 random primers, 20 produced polymorphic bands. The primers, OPM-14 and OPAW-13, produced a minimum number of bands (3) each across the ten mutants, while the primer OPF-13 produced the maximum number of bands (10), followed by the primers OPU-13, OPAM-06, OPAW-09 and OPD-05, which produced 9 bands each. The number of amplicons varied from 3 to 10, with an average of 7 bands, out of which 4.57 were polymorphic. The percentage of polymorphism ranged from 0.00 to 100 with an average of 57%. In the present study, RAPD markers were found most polymorphic, with an average polymorphism information content (PIC) value of 0.347, effective multiplex ratio (EMR) of 35.14, marker index (MI) of 14.19, resolution power (Rp) of 11.19, effective marker index (EMI) of 8.21 and genotype index (GI) of 0.36, indicating that random primers are useful in studies of genetic characterization in J. curcas mutant plants. In a dendrogram constructed based on Jaccard's similarity coefficients, the mutants were grouped into three main clusters viz., (a) control, 10 Kr, 15 Kr, 20 Kr, 2% EMS, and 3% EMS, (b) 5 Kr and 1% EMS, and (c) 25 Kr and 4% EMS mutants. Based on the attributes of the random primers and polymorphism studied, it is concluded that RAPD analysis offers a useful molecular marker for the identification of the mutants in gamma rays and EMS treated plants.
authors.affiliation: Department of Botany, Government Arts College (affiliated to Thiruvalluvar University), Thiruvannamalai 606 603, Tamilnadu, India; Division of Biotechnology and Molecular Biology, Department of Botany, Annamalai University, Annamalainagar 608 002, Tamilnadu, India. Electronic address: biofueldd2009@yahoo.com.
authors.forename: Dharman
authors.initials: D
authors.lastname: Dhakshanamoorthy
authors.affiliation: Division of Biotechnology and Molecular Biology, Department of Botany, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
authors.forename: Radhakrishnan
authors.initials: R
authors.lastname: Selvaraj
authors.affiliation: Division of Biotechnology and Molecular Biology, Department of Botany, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
authors.forename: Alagappan
authors.initials: A
authors.lastname: Chidambaram
mesh_terms: D017931:DNA Primers; D018744:DNA, Plant / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005020:Ethyl Methanesulfonate / Q000008:administration & dosage / Q000494:pharmacology*; D005720:Gamma Rays; D014644:Genetic Variation; D031286:Jatropha / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique; D012639:Seeds / Q000187:drug effects / Q000528:radiation effects
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D009153:Mutagens; D005020:Ethyl Methanesulfonate
keywords: Ethyl methane sulphonate (EMS); Gamma irradiation; Induced mutagenesis; Jatropha curcas; RAPD marker