title: Synthesis, characterization, antimicrobial, DNA binding and cleavage studies of mixed ligand Cu(II), Co(II) complexes.
abstract: The mixed ligand complexes MLA of Cu(II) and Co(II) with Schiff base derived from 4-amino antipyrine and 5-NO(2) salicylaldehyde (2,3 -dimethyl-1-phenyl-4-(2-hydroxy-5-nitro benzylideneamino)-pyrazol-5-one) as ONO donor (L) and A = 2,2 bipyridine (bpy),1,10 phenonthroline (1,10 phen) as N, N donor ligands have been prepared, owing to their biological and other applications. The structural features have arrived from their elemental analyses, magnetic susceptibility, molar conductance, Mass, IR, UV-VIS, powdered XRD and ESR spectral studies, that established MLA type of composition for the metal complexes. The electronic absorption spectral data of the complexes suggest an octahedral geometry around the central metal ion. The interaction of the complexes with Calf Thymus (CT) DNA has been studied using absorption spectra, viscosity measurements and fluorescence spectra. The binding constants (K(b)) of the complexes were determined as 2.1 × 10(6) M(-1) for complex 1, 2.5x10(6)M(-1) for complex 2, 1.16 × 10(6) M(-1) for complex 3,1.25x10(6)M(-1) for complex 4, DNA cleavage experiments performed on pBR-322 plasmids using metal complexes in the presence of H(2)O(2) showed that all the complexes afford a pronounced DNA cleavage. Molecular modelling studies were also performed to confirm the geometries of the complexes. The ligand and their metal complexes were screened for their antimicrobial activity against bacteria. The results showed that the metal complexes are biologically active.
authors.affiliation: Department of Chemistry, Osmania University, Hyderabad, 500007, India.
authors.forename: D Shiva
authors.initials: DS
authors.lastname: Leela
authors.forename: B
authors.initials: B
authors.lastname: Ushaiah
authors.forename: G
authors.initials: G
authors.lastname: Anupama
authors.forename: M
authors.initials: M
authors.lastname: Sunitha
authors.forename: C Gyana
authors.initials: CG
authors.lastname: Kumari
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D000983:Antipyrine / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D003035:Cobalt / Q000737:chemistry*; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000737:chemistry; D060328:Magnetic Phenomena; D008958:Models, Molecular; D008968:Molecular Conformation; D010618:Phenanthrolines / Q000737:chemistry; D011720:Pyrazoles / Q000737:chemistry; D012545:Schiff Bases / Q000737:chemistry; D014783:Viscosity
chemical_list: D000900:Anti-Bacterial Agents; D056831:Coordination Complexes; D010618:Phenanthrolines; D011720:Pyrazoles; D012545:Schiff Bases; D003035:Cobalt; C031280:pyrazole; D003300:Copper; D004247:DNA; C427203:calf thymus DNA; D006861:Hydrogen Peroxide; D000983:Antipyrine; C025205:1,10-phenanthroline