title: [Microevolution of cholera agent in the modern period].
abstract: OBJECTIVE
  To carry out comparative molecular genetic analysis of highly pathogenic atypical Vibrio cholerae strains biovar El Tor, isolated in the territory of RF, in order to determine micro-evolutionary alterations of cholera agent in the modern period.
  
  
  METHODS
  38 clinical strains have been examined by means of polymerase chain reaction, sequencing and MLVA-analysis. The selected strains were isolated at different periods of time during cholera epidemic complications and differed between each other in virulence.
  
  
  RESULTS
  It is demonstrated that new variants have emerged in the course of short-term microevolution. Their genome structure and function differ from those of all previously known strains. The genome alterations have been caused by point mutations in ctxB u tcpA genes associated with virulence and located in CTXΦ prophage and pathogenicity island VPI-1 respectively, as well as by the extended deletion in pandemicity island VSP-II. Presented is the dynamics of genome structure and function alterations in modern strains.
  
  
  CONCLUSIONS
  The discovered genomic alterations in the new variants of the agent evolved in the process of microevolution are indicative of their epidemic potential enhancement and probability of virulence potentiation.
authors.forename: N I
authors.initials: NI
authors.lastname: Smirnova
authors.forename: D A
authors.initials: DA
authors.lastname: Agafonov
authors.forename: T A
authors.initials: TA
authors.lastname: Kul'shan'
authors.forename: Ia M
authors.initials: IaM
authors.lastname: Krasnov
authors.forename: V V
authors.initials: VV
authors.lastname: Kutyrev
mesh_terms: D000595:Amino Acid Sequence; D002771:Cholera / Q000453:epidemiology / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics*; D004196:Disease Outbreaks / Q000517:prevention & control / Q000706:statistics & numerical data; D019143:Evolution, Molecular; D036781:Fimbriae Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D056726:Genetic Association Studies; D014644:Genetic Variation; D006801:Humans; D012426:Russia / Q000453:epidemiology; D065288:Serogroup; D014734:Vibrio cholerae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity
chemical_list: C085807:TcpA protein, Vibrio cholerae; D036781:Fimbriae Proteins; D002772:Cholera Toxin