title: Development of a functional cell-based assay that probes the specific interaction between influenza A virus NP and its packaging signal sequence RNA.
abstract: Although cis-acting packaging signal RNA sequences for the influenza virus NP encoding vRNA have been identified recently though genetic studies, little is known about the interaction between NP and the vRNA packaging signals either in vivo or in vitro. Here, we provide evidence that NP is able to interact specifically with the vRNA packaging sequence RNA within living cells and that the specific RNA binding activity of NP in vivo requires both the N-terminal and central region of the protein. This assay established would be a valuable tool for further detailed studies of the NP-packaging signal RNA interaction in living cells.
authors.affiliation: National Research Laboratory of Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea, Seocho-gu, Banpo-dong 505, Seoul 137-701, Republic of Korea.
authors.forename: Jiwon
authors.initials: J
authors.lastname: Woo
authors.affiliation: National Research Laboratory of Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea, Seocho-gu, Banpo-dong 505, Seoul 137-701, Republic of Korea.
authors.forename: Kyung Lee
authors.initials: KL
authors.lastname: Yu
authors.affiliation: Avixgen Inc., Seocho-gu, Banpo-dong 505, Seoul 137-701, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: National Research Laboratory of Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea, Seocho-gu, Banpo-dong 505, Seoul 137-701, Republic of Korea. Electronic address: jiyou@catholic.ac.kr.
authors.forename: Ji Chang
authors.initials: JC
authors.lastname: You
mesh_terms: D001681:Biological Assay / Q000379:methods*; D017353:Gene Deletion; D009980:Influenza A virus / Q000378:metabolism*; D007700:Kinetics; D007763:Lac Operon; D009698:Nucleoproteins / Q000378:metabolism*; D011485:Protein Binding; D021382:Protein Sorting Signals; D012367:RNA, Viral / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D009698:Nucleoproteins; D021382:Protein Sorting Signals; D012367:RNA, Viral
keywords: Cell-based protein–RNA interaction; Influenza; Nucleoprotein; Packaging signal sequence