title: Super-resolution imaging of nuclear bodies by STED microscopy.
abstract: The sizes of nuclear bodies and other nuclear structures are normally no more than a few hundred nanometers. This size is below the resolution limit of light microscopy and thus requires electron microscopy for direct observation. Recent developments in super-resolution microscopy have extended the resolution of light microscopy to beyond 100 nm. Here, we describe a super-resolution technique, gated STED, for the analysis of the structure of nuclear bodies, with emphasis on the sample preparation and other technical tips that are important to obtain high-quality super-resolution images.
authors.affiliation: Laboratory for Cell Polarity Regulation, RIKEN Quantitative Biology Center, 6-2-3 Furuedai, Suita, Osaka, 565-0824, Japan, y.okada@riken.jp.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okada
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D007091:Image Processing, Computer-Assisted / Q000295:instrumentation; D045586:Intranuclear Inclusion Bodies / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*