title: Vitamin D receptor gene polymorphisms and steroid receptor status among Saudi women with breast cancer.
abstract: The vitamin D receptor (VDR) is a mediator for the cellular effects of vitamin D and interacts with other cell signaling pathways that influence cancer development. We evaluated the associations of the FOK1 and Taq1 VDR polymorphisms and breast cancer risk and possible effect modification by steroid receptor status of the tumor. This case-control study includes 95 breast cancer patients and 100 age-matched controls. Genotyping for VDR FOK1 and Taq1 polymorphisms was performed using polymerase chain reaction-based restriction fragment length polymorphism. Level of 25(OH)D in serum was determined using ELISA. Immunohistochemical studies were performed for estrogen receptors (ER) and progesterone receptors (PR). The frequencies of ff genotype were significantly increased in the breast cancer group compared to the control group. Carriers of the f allele were significantly more likely to develop BC. We observed a statistically significant interaction for the Fok1 polymorphism and ER status. Our results demonstrated that FOK1 f. genotype and f allele have an important role in breast cancer risk in Saudi patients.
authors.affiliation: Department of Pathology and Cytopathology, College of Medicine, Taif University, Saudi Arabia; Laboratory and Blood Bank, King Abdul Aziz Hospital, Taif, Saudi Arabia.
authors.forename: Dalal M
authors.initials: DM
authors.lastname: Nemenqani
authors.affiliation: Department of Biochemistry, college of Medicine, Taif University, Al Taif, Saudi Arabia; Departments of Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: rehab_atta@yahoo.com.
authors.forename: Rehab A
authors.initials: RA
authors.lastname: Karam
authors.affiliation: Department of Anatomy and Histology, College of Medicine, Taif University, Al Taif, Saudi Arabia; Departments of Histology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Mona G
authors.initials: MG
authors.lastname: Amer
authors.affiliation: Department of surgery, College of Medicine, Taif University, Al Taif, Saudi Arabia; Department of surgery, Benha Teaching Hospital, Benha, Egypt.
authors.forename: Tamer M
authors.initials: TM
authors.lastname: Abd El Rahman
mesh_terms: D000328:Adult; D001943:Breast Neoplasms / Q000453:epidemiology / Q000235:genetics*; D002112:Calcifediol / Q000097:blood; D016022:Case-Control Studies; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D018167:Receptors, Calcitriol / Q000235:genetics*; D011960:Receptors, Estrogen / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D012529:Saudi Arabia / Q000453:epidemiology
chemical_list: D018167:Receptors, Calcitriol; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; C579457:VDR protein, human; C035042:endodeoxyribonuclease FokI; D015252:Deoxyribonucleases, Type II Site-Specific; C027046:TCGA-specific type II deoxyribonucleases; D002112:Calcifediol
keywords: Breast cancer; Single nucleotide polymorphisms; Vitamin D receptor