title: Selection of suitably non-repressing carbon sources for expression of alcohol oxidase isozyme promoters in the methylotrophic yeast Pichia methanolica.
abstract: In this work, we aimed to select suitable non-repressing carbon sources for the expression of promoters derived from the alcohol oxidase (AOD) isozyme genes, PMOD1 and PMOD2, during the growth of Pichia methanolica. Our results revealed that xylose is the best non-repressing carbon source for heterologous gene expression using both PMOD1 and PMOD2, and that glycerol is also a suitable carbon source with by which the on/off of PMOD2 expression can be controlled.
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. Electronic address: t_nakaga@gifu-u.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Keishi
authors.initials: K
authors.lastname: Wakayama
authors.affiliation: Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D002244:Carbon / Q000378:metabolism* / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D010843:Pichia / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics*
chemical_list: D007527:Isoenzymes; D002244:Carbon; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase
keywords: Alcohol oxidase isozymes; Gene expression; MOD promoters; Non-repressing carbon source; Pichia methaolica