title: Oxidative damage induced by copper and beta-cypermethrin in gill of the freshwater crayfish Procambarus clarkii.
abstract: Copper (Cu) and pyrethroid are common contaminants found in the aquatic environment, and their potential toxicological effects on aquatic organisms have received extensive attention. However, the impact on crayfish species of exposure to the two chemicals are still largely unknown. The current study assessed the sublethal toxicities induced by Cu and commercial formulation of beta-cypermethrin in the freshwater crayfish Procambarus clarkii. The static test method of acute toxicity test was used. Five biomarkers of oxidative effects, i.e. reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and protein carbonyls, and pathologic changes were determined. The results demonstrated that there was a significant increase in the contents of ROS, MDA and protein carbonyls accompanied by markedly decreased SOD and CAT activities in a concentration-dependent manner. The linear relationship between protein carbonyls induction and MDA formation was observed evidently in crayfish gills at 96h. Higher contents of ROS enhanced the risk of lipid peroxidation and protein carbonylation, thus impacting vital physiological functions of gills. The results indicated that protein oxidation may be one of the main mechanisms of pollution-induced toxicity to crayfish gills. Protein carbonyl levels could be considered as a potential biomarker of exposure to environmental contaminants.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, People's Republic of China. Electronic address: kqwei88@aliyun.com.
authors.forename: Keqiang
authors.initials: K
authors.lastname: Wei
authors.affiliation: School of Economics and Management, Shanxi University, Taiyuan 030006, People's Republic of China.
authors.forename: Junxian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000633:toxicity*; D005618:Fresh Water; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D050050:Protein Carbonylation; D011722:Pyrethrins / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity; D014877:Water Pollution, Chemical / Q000009:adverse effects
chemical_list: D011722:Pyrethrins; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; C017160:cypermethrin; D008315:Malondialdehyde; D003300:Copper; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Beta-cypermethrin; Copper; Procambarus clarkii; Protein carbonyl; Reactive oxygen species (ROS)