title: Tarantula cubensis extract alters the degree of apoptosis and mitosis in canine mammary adenocarcinomas.
abstract: In the present study, 13 clinical cases of canine mammary adenocarcinoma were evaluated in order to understand the effect of Tarantula cubensis extract (TCE) on tumor tissue. Punch biopsies were taken from the tumors before treatment with TCE. Subcutaneous injections of TCE were administered three times at weekly intervals (3 mL per dog). Between days 7 and 10 after the third injection, the tumor masses were extirpated by complete unilateral mastectomy. Pre- and post-treatment tumor tissues were immunohistochemically assessed. The expression of B-cell lymphoma 2 (Bcl-2) was found to be higher in pre-treatment compared to post-treatment tissues (p < 0.01) whereas Ki-67 expression was lower in post-treatment tissues (p < 0.01). No significant differences in fibroblast growth factor or vascular endothelial growth factor expression were observed between pre- and post-treatment tissues (p > 0.05). The apoptotic index was determined to be low before treatment and increased during treatment. These results suggest that TCE may be effective for controlling the local growth of canine mammary adenocarcinoma by regulating apoptosis.
authors.affiliation: Departments of Obstetrics and Gynecology, University of Ondokuz Mayis, Samsun 55200, Turkey.nilgung@omu.edu.tr.
authors.forename: Nilgun
authors.initials: N
authors.lastname: Gultiken
authors.forename: Tolga
authors.initials: T
authors.lastname: Guvenc
authors.forename: Duygu
authors.initials: D
authors.lastname: Kaya
authors.forename: Ali Reha
authors.initials: AR
authors.lastname: Agaoglu
authors.forename: Serhan Serhat
authors.initials: SS
authors.lastname: Ay
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Kucukaslan
authors.forename: Birten
authors.initials: B
authors.lastname: Emre
authors.forename: Murat
authors.initials: M
authors.lastname: Findik
authors.forename: Sabine
authors.initials: S
authors.lastname: Schäfer-Somi
authors.forename: Selim
authors.initials: S
authors.lastname: Aslan
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000503:physiopathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004283:Dog Diseases / Q000188:drug therapy* / Q000503:physiopathology; D004285:Dogs; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000188:drug therapy* / Q000503:physiopathology; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D008938:Mitosis / Q000187:drug effects; D013112:Spiders / Q000737:chemistry*
keywords: Tarantula cubensis; apoptosis; canine; mammary tumors