title: Sulfated galactofucan from the brown alga Saccharina latissima--variability of yield, structural composition and bioactivity.
abstract: The fucose-containing sulfated polysaccharides (SP) from brown algae exhibit a wide range of bioactivities and are, therefore, considered promising candidates for health-supporting and medicinal applications. A critical issue is their availability in high, reproducible quality. The aim of the present study was to fractionate and characterize the SP extracted from Saccharina latissima (S.l.-SP) harvested from two marine habitats, the Baltic Sea and North Atlantic Ocean, in May, June and September. The fractionation of crude S.l.-SP by anion exchange chromatography including analytical investigations revealed that S.l.-SP is composed of a homogeneous fraction of sulfated galactofucan (SGF) and a mixture of low-sulfated, uronic acid and protein containing heteropolysaccharides. Furthermore, the results indicated that S.l. growing at an intertidal zone with high salinity harvested at the end of the growing period delivered the highest yield of S.l.-SP with SGF as the main fraction (67%). Its SGF had the highest degree of sulfation (0.81), fucose content (86.1%) and fucose/galactose ratio (7.8) and was most active (e.g., elastase inhibition: IC50 0.21 μg/mL). Thus, S.l. from the North Atlantic harvested in autumn proved to be more appropriate for the isolation of S.l.-SP than S.l. from the Baltic Sea and S.l. harvested in spring, respectively. In conclusion, this study demonstrated that habitat and harvest time of brown algae should be considered as factors influencing the yield as well as the composition and thus also the bioactivity of their SP.
authors.affiliation: . kehrig@pharmazie.uni-kiel.de.
authors.forename: Karina
authors.initials: K
authors.lastname: Ehrig
authors.affiliation: . salban@pharmazie.uni-kiel.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Alban
mesh_terms: D002458:Cell Fractionation; D002852:Chromatography, Ion Exchange; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007795:Laminaria / Q000737:chemistry*; D015394:Molecular Structure; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors
chemical_list: D005685:Galactans; D010196:Pancreatic Elastase