title: Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements.
abstract: In the last 15 years, there has been an explosion in the development of genetically encoded biosensors that report enzyme activity, chemical transformation, or concentration of ions and molecules in living cells. Currently, there are well over 120 biosensors of different cellular targets. As a general design principle, these sensors convert a molecular event, such as the binding of a molecule to a sensing domain or a signal-induced change in protein conformation, into a change in the sensor's fluorescence properties. In contrast to small-molecule sensors, genetically encoded sensors are generated when sensor-encoding nucleic acid sequences, which have been introduced by transgenic technologies, are translated in cells, tissues, or organisms. One of the best developed classes of biosensors is the genetically encoded Ca(2+) indicators (GECIs). Here, we briefly summarize the properties of ratiometric GECIs and describe how they are used to quantify Ca(2+) in specific cellular locations, such as the cytosol, nucleus, endoplasmic reticulum, and mitochondria.
authors.affiliation: Department of Chemistry and Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, Colorado 80309.
authors.forename: J Genevieve
authors.initials: JG
authors.lastname: Park
authors.affiliation: Department of Chemistry and Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, Colorado 80309.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Palmer
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D015388:Organelles / Q000378:metabolism*; D011487:Protein Conformation; D015202:Protein Engineering
chemical_list: D008164:Luminescent Proteins; D002118:Calcium