title: A novel laccase with potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from mycelia of mushroom Coprinus comatus.
abstract: A novel laccase was isolated and purified from fermentation mycelia of mushroom Coprinus comatus with an isolation procedure including three ion-exchange chromatography steps on DEAE-cellulose, CM-cellulose, and Q-Sepharose and one gel-filtration step by fast protein liquid chromatography on Superdex 75. The purified enzyme was a monomeric protein with a molecular weight of 64 kDa. It possessed a unique N-terminal amino acid sequence of AIGPVADLKV, which has considerably high sequence similarity with that of other fungal laccases, but is different from that of C. comatus laccases reported. The enzyme manifested an optimal pH value of 2.0 and an optimal temperature of 60°C using 2,2'-azinobis(3-ethylbenzothiazolone-6-sulfonic acid) diammonium salt (ABTS) as the substrate. The laccase displayed, at pH 2.0 and 37°C, K(m) values of 1.59 mM towards ABTS. It potently suppressed proliferation of tumor cell lines HepG2 and MCF7, and inhibited human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) with an IC50 value of 3.46 μM, 4.95 μM, and 5.85 μM, respectively, signifying that it is an antipathogenic protein.
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Cheng-Bo
authors.initials: CB
authors.lastname: Rong
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Kong
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Qian-Jiang
authors.initials: QJ
authors.lastname: Miao
authors.affiliation: Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing Engineering Research Center for Edible Mushroom, Beijing 100097, China.
authors.forename: Shou-Xian
authors.initials: SX
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.
authors.forename: He-Xiang
authors.initials: HX
authors.lastname: Wang
authors.affiliation: Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Sciences and Engineering, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Guo-Qing
authors.identifier: 0000-0002-6832-5931
authors.initials: GQ
authors.lastname: Zhang
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D003302:Coprinus / Q000737:chemistry / Q000201:enzymology*; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology; D054303:HIV Reverse Transcriptase / Q000096:biosynthesis / Q000187:drug effects; D015497:HIV-1 / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D042845:Laccase / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D061986:MCF-7 Cells; D025282:Mycelium / Q000737:chemistry / Q000201:enzymology; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology
chemical_list: D042845:Laccase; C514824:reverse transcriptase, Human immunodeficiency virus 1; D054303:HIV Reverse Transcriptase