title: Induction of apoptosis in Caco-2 cells by exogenously added O₂⁻ produced by a nanodevice.
abstract: The effects of reactive oxygen species on cells have attracted considerable attention in relation to oxidative stress and related disorders. Superoxide (O2(-)) is the primary reactive oxygen species formed in animals as a byproduct or purposeful product of enzymes. We recently established an O2(-)-generating nanodevice that produces O2(-) continuously even in culture medium, by improving an original nanodevice. The new nanodevice, named Device II, efficiently induced cell death in Caco-2 cells in a time- and dose-dependent manner. Catalase largely recovered the cell viability, while superoxide dismutase rather lowered the viability. Flow cytometric and fluorescence microscopic analyses revealed that phosphatidylserine was exposed on the cells and that caspase-3 was activated in the cells after treatment with Device II. These findings indicated that exogenously added O2(-) caused apoptosis in Caco-2 cells through its derivative H2O2.
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yoshioka
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan.
authors.forename: Hironosuke
authors.initials: H
authors.lastname: Fujibayashi
authors.affiliation: INCS Shigenobu Station, Ehime University, Toon, Ehime 791-0295, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kameda
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan.
authors.forename: Daijiro
authors.initials: D
authors.lastname: Kan
authors.affiliation: Department of Biochemistry, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan.
authors.forename: Shigenobu
authors.initials: S
authors.lastname: Tone
authors.affiliation: Department of Applied Chemistry, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan. Electronic address: tamura@ehime-u.ac.jp.
authors.forename: Minoru
authors.initials: M
authors.lastname: Tamura
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D018938:Caco-2 Cells; D053148:Caspase 3 / Q000096:biosynthesis; D002374:Catalase / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010716:Phosphatidylinositols / Q000378:metabolism; D010718:Phosphatidylserines; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D008562:Membrane Glycoproteins; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D013481:Superoxides; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; C056558:CYBB protein, human; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; D053148:Caspase 3
keywords: Apoptosis; Caco-2; Nanodevice; Oxidative stress; Phosphatidylserine; Superoxide