title: Fluorescent labeling of SNAP-tagged proteins in cells.
abstract: One of the most prominent self-labeling tags is SNAP-tag. It is an in vitro evolution product of the human DNA repair protein O (6)-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically with benzylguanine (BG) and benzylchloropyrimidine (CP) derivatives, leading to covalent labeling of SNAP-tag with a synthetic probe (Gronemeyer et al., Protein Eng Des Sel 19:309-316, 2006; Curr Opin Biotechnol 16:453-458, 2005; Keppler et al., Nat Biotechnol 21:86-89, 2003; Proc Natl Acad Sci U S A 101:9955-9959, 2004). SNAP-tag is well suited for the analysis and quantification of fused target protein using fluorescence microscopy techniques. It provides a simple, robust, and versatile approach to the imaging of fusion proteins under a wide range of experimental conditions.
authors.affiliation: Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
authors.forename: Gražvydas
authors.initials: G
authors.lastname: Lukinavičius
authors.forename: Luc
authors.initials: L
authors.lastname: Reymond
authors.forename: Kai
authors.initials: K
authors.lastname: Johnsson
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D013194:Staining and Labeling
chemical_list: D005456:Fluorescent Dyes; D011993:Recombinant Fusion Proteins