title: Magnetic apatite for structural insights on the plasma membrane.
abstract: The iron oxide-hydroxyapatite (FeOxHA) nanoparticles reported here differ from those reported before by their advantage of homogeneity and simple preparation; moreover, the presence of carboxymethyldextran (CMD), together with hydroxyapatite (HA), allows access to the cellular membrane, which makes our magnetic apatite unique. These nanoparticles combine magnetic behavior, Raman label ability and the property of interaction with the cellular membrane; they therefore represent an interesting material for structural differentiation of the cell membrane. It was observed by Raman spectroscopy, scanning electron microscopy (SEM) and fluorescence microscopy that FeOxHA adheres to the plasma membrane and does not penetrate the membrane. These insights make the nanoparticles a promising material for magnetic cell sorting, e.g. in microfluidic device applications.
authors.affiliation: Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745 Jena, Germany.
authors.forename: Sarmiza E
authors.initials: SE
authors.lastname: Stanca
authors.forename: Robert
authors.initials: R
authors.lastname: Müller
authors.forename: Jan
authors.initials: J
authors.lastname: Dellith
authors.forename: Sandor
authors.initials: S
authors.lastname: Nietzsche
authors.forename: Stephan
authors.initials: S
authors.lastname: Stöckel
authors.forename: Christoph
authors.initials: C
authors.lastname: Biskup
authors.forename: Volker
authors.initials: V
authors.lastname: Deckert
authors.forename: Christoph
authors.initials: C
authors.lastname: Krafft
authors.forename: Jürgen
authors.initials: J
authors.lastname: Popp
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000648:ultrastructure; D003911:Dextrans / Q000737:chemistry; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D060328:Magnetic Phenomena; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D013059:Spectrum Analysis, Raman
chemical_list: D003911:Dextrans; D005290:Ferric Compounds; D006882:Hydroxyapatites; C000499:ferric oxide; C014392:carboxymethyl dextran