title: Isothermal DNA origami folding: avoiding denaturing conditions for one-pot, hybrid-component annealing.
abstract: The DNA origami technique offers great potential for nanotechnology. Using biomolecular self-assembly, defined 2D and 3D nanoscale DNA structures can be realized. DNA origami allows the positioning of proteins, fluorophores or nanoparticles with an accuracy of a few nanometers and enables thereby novel nanoscale devices. Origami assembly usually includes a thermal denaturation step at 90 °C. Additional components used for nanoscale assembly (such as proteins) are often thermosensitive, and possibly damaged by such harsh conditions. They have therefore to be attached in an extra second step to avoid defects. To enable a streamlined one-step nanoscale synthesis - a so called one-pot folding - an adaptation of the folding procedures is required. Here we present a thermal optimization of this process for a 2D DNA rectangle-shaped origami resulting in an isothermal assembly protocol below 60 °C without thermal denaturation. Moreover, a room temperature protocol is presented using the chemical additive betaine, which is biocompatible in contrast to chemical denaturing approaches reported previously.
authors.affiliation: Leibniz Institute of Photonic Technology (IPHT), Jena 07745, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kopielski
authors.forename: Anne
authors.initials: A
authors.lastname: Schneider
authors.forename: Andrea
authors.initials: A
authors.lastname: Csáki
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Fritzsche
mesh_terms: D004247:DNA / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D044366:Transition Temperature
chemical_list: D004247:DNA