title: Glucose transport machinery reconstituted in cell models.
abstract: Here we demonstrate the production of a functioning cell model by formation of giant vesicles reconstituted with the GLUT1 glucose transporter and a glucose oxidase and hydrogen peroxidase linked fluorescent reporter internally. Hence, a simplified artificial cell is formed that is able to take up glucose and process it.
authors.affiliation: Department of Experimental Medical Science, Faculty of Medicine, Lund University, Box 117, SE-22100, Lund, Sweden. karin.lindkvist@med.lu.se.
authors.forename: Jesper S
authors.initials: JS
authors.lastname: Hansen
authors.forename: Karin
authors.initials: K
authors.lastname: Elbing
authors.forename: James R
authors.initials: JR
authors.lastname: Thompson
authors.forename: Noah
authors.initials: N
authors.lastname: Malmstadt
authors.forename: Karin
authors.initials: K
authors.lastname: Lindkvist-Petersson
mesh_terms: D001692:Biological Transport; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D051272:Glucose Transporter Type 1 / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D053835:Unilamellar Liposomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D051272:Glucose Transporter Type 1; D010713:Phosphatidylcholines; D053835:Unilamellar Liposomes; C021016:1,2-diphytanoylphosphatidylcholine; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose