title: Morphological and phylogenetic analysis of Nosema sp. HR (Microsporidia, Nosematidae): a new microsporidian pathogen of Histia rhodope Cramer (Lepidoptera, Zygaenidae).
abstract: A new microsporidium was isolated from Histia rhodope Cramer (Lepidoptera, Zygaenidae), a pest of Bischofia javanica BL. in China. The morphology and molecular systematic of this novel microsporidian isolate had been described in this study. The spores were long oval and measured 3.1 × 1.9 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: 14-15 polar filament coils, posterior vacuole, and a diplokaryon. The sequenced rRNA gene of this isolate is 4309 bp long. The organization of the rRNA gene is 5'-LSU rRNA-ITS-SSU rRNA-IGS-5S-3', which is similar to that of other Nosema species (such as Nosema bombycis). Phylogenetic analysis based on LSU rRNA gene and SSU rRNA gene both revealed that this novel micorsporidian which isolated from H. rhodope had close relationship to the genus Nosema. Additionally, this isolate can also cause systemic infection of Bombyx mori. So, we should pay attention not only to N. bombycis, but also to other microsporidian (such as Nosema sp. HR) in sericulture in the future.
authors.affiliation: Experimental Teaching Center, Chongqing Medical University, Yuzhong, Chongqing, 400016, People's Republic of China, lhd20052008@126.com.
authors.forename: Handeng
authors.initials: H
authors.lastname: Liu
authors.forename: Songtao
authors.initials: S
authors.lastname: Ding
authors.forename: Qizhong
authors.initials: Q
authors.lastname: Qin
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.forename: Huimin
authors.initials: H
authors.lastname: Peng
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000469:parasitology; D002080:Butterflies / Q000469:parasitology*; D002392:Catechin / Q000031:analogs & derivatives; D002681:China; D054884:Host-Pathogen Interactions; D016818:Nosema / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D013172:Spores, Fungal / Q000648:ultrastructure
chemical_list: D012335:RNA, Ribosomal; C532536:Rhodopes; D002392:Catechin