title: Changes in the levels of off-flavor generation in soybean through biotic elicitor treatments.
abstract: The nutritional benefits of soybean remain underutilized as the off-flavor present in it limits the consumption and acceptability among people. The aim of the present study was to unveil the effect of the phytohormones methyl jasmonate (MJ: 0, 50 μM, 1 mM, and 15 mM) and salicylic acid (SA: 0, 50 μM, 0.1 mM, and 10 mM) as elicitors on two contrasting off-flavor soybean varieties at different growth stages (1, bloom; 2, pod development; 3, seed development). The effects of two elicitors varied widely and were found to be dose dependent and growth stage independent. SA reduces the lipoxygenase (LOX) and hydroperoxide lyase (HPL) activity, which in turn resulted in reduction in the TBA number and carbonyl value in contrast to MJ. SA 0.1 mM is the most effective dose in reduction of off-flavor determining parameters and protein oxidation, and it reduces the LOX and HPL activity by 2.3- and 2.4-fold, respectively in "high off-flavor" cultivar 'Bragg' compared to "low off-flavor" cultivar 'DS 2706' which showed 1.4- and 2.1-fold, respectively. This reduction in protein oxidation is also supported by enhanced content of antioxidant enzymes. Thus, phytohormone SA can be used in reduction of off-flavor generation, more effectively than MJ treatments, in soybean.
authors.affiliation: Division of Biochemistry, ‡Division of Genetics, and §Division of Food Science & Post harvest Technology, Indian Agricultural Research Institute , New Delhi-110012, India.
authors.forename: Sweta
authors.initials: S
authors.lastname: Kumari
authors.forename: Anil
authors.initials: A
authors.lastname: Dahuja
authors.forename: T
authors.initials: T
authors.lastname: Vinutha
authors.forename: Sanjay Kumar
authors.initials: SK
authors.lastname: Lal
authors.forename: Abhijit
authors.initials: A
authors.lastname: Kar
authors.forename: Raj D
authors.initials: RD
authors.lastname: Rai
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000446:Aldehyde-Lyases / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005421:Flavoring Agents / Q000032:analysis* / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D010084:Oxidation-Reduction; D054883:Oxylipins / Q000494:pharmacology*; D010940:Plant Proteins / Q000378:metabolism; D020156:Salicylic Acid / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D005421:Flavoring Agents; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; D003577:Cytochrome P-450 Enzyme System; D008084:Lipoxygenase; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase; D020156:Salicylic Acid
keywords: antioxidant enzymes; elicitation; off-flavor; protein oxidation; soybean