title: The effect of leukocyte-reduced platelet-rich plasma on the proliferation of autologous adipose-tissue derived mesenchymal stem cells.
abstract: Clinical application of platelet-rich plasma (PRP) and stem cells has become more and more important in regenerative medicine during the last decade. However, differences in PRP preparations may contribute to variable PRP compositions with unpredictable effects on a cellular level. In the present study, we modified the centrifugation settings in order to provide a leukocyte-reduced PRP and evaluated the interactions between PRP and adipose-tissue derived mesenchymal stem cells (ASCs).PRP was obtained after modification of three different centrifugation settings and investigated by hemogram analysis, quantification of protein content and growth factor concentration. ASCs were cultured in serum-free α-MEM supplemented with autologous 10% or 20% leukocyte-reduced PRP. Cell cycle kinetics of ASCs were analyzed using flow cytometric analyses after 48 hours.Thrombocytes in PRP were concentrated, whereas erythrocytes, and white blood cells (WBC) were reduced, independent of centrifugation settings. Disabling the brake further reduced the number of WBCs. A higher percentage of cells in the S-phase in the presence of 20% PRP in comparison to 10% PRP and 20% fetal calf serum (FCS) advocates the proliferation stimulation of ASCs.These findings clearly demonstrate considerable differences between three PRP separation settings and assist in safeguarding the combination of leukocyte-reduced PRP and stem cells for regenerative therapies.
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Loibl
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Siegmund
authors.initials: S
authors.lastname: Lang
authors.affiliation: Department of Obstetrics and Gynecology, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Gero
authors.initials: G
authors.lastname: Brockhoff
authors.affiliation: AO Research Institute Davos, Davos, Switzerland.
authors.forename: Boyko
authors.initials: B
authors.lastname: Gueorguiev
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Franz
authors.initials: F
authors.lastname: Hilber
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Worlicek
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Baumann
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Grechenig
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Johannes
authors.initials: J
authors.lastname: Zellner
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Michaela
authors.initials: M
authors.lastname: Huber
authors.affiliation: Department of Orthopedic Surgery, University Hospital Basel, Basel, Switzerland.
authors.forename: Victor
authors.initials: V
authors.lastname: Valderrabano
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Angele
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Nerlich
authors.affiliation: Center of Plastic, Hand and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Prantl
authors.affiliation: Department of Trauma Surgery, University Medical Center Regensburg, Regensburg, Germany.|Department of Orthopedic Surgery, University Hospital Basel, Basel, Switzerland.|Center of Plastic, Hand and Reconstructive Surgery, University Medical Center Regensburg, Regensburg, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Gehmert
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D055815:Young Adult
keywords: PRP; Platelet-rich plasma; adipose-tissue derived mesenchymal stem cells; cell cycle; growth factor; white blood cells