title: Early hemorheological changes in a porcine model of intravenously given E. coli induced fulminant sepsis.
abstract: The pathophysiology of hemorheological and microcirculatory disturbances in septic process -mostly during the early hours- still not clarified in all the details, yet. In anesthetized pigs living E. coli (ATCC 25922 strain) was administered intravenously with an increasing concentration and the animals were observed for 8 hours. Before the intervention and in every 2 hours arterial (cannulated femoral artery) and venous (cannulated external jugular vein) blood samples were collected for hemorheological laboratory tests: blood and plasma viscosity, ESR, leukocyte anti-sedimentation rate, erythrocyte deformability (together with osmoscan parameters) and erythrocyte aggregation (using light-transmission and laser back-scattering methods) Control animals were stable over the 8-hour anesthesia, while septic animals died by the 6th hours in a fulminant sepsis. Over the experimental period, the tendency of impairment in erythrocyte deformability (together with osmotic gradient ektacytometry parameters) and the controversial decreasing of erythrocyte aggregation values (declining all aggregation index values, elongating t1/2) were well detected in this porcine model during the early hours (4- 6) of fulminant sepsis. The in vitro effect of these bacteria on erythrocytes' micro-rheological parameter was similar: decreasing red blood cell deformability and lowering aggregation. Further studies are needed to clarify the early micro-rheological changes of bacteremia and the developing sepsis.
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, University of Debrecen, Hungary.
authors.forename: Norbert
authors.initials: N
authors.lastname: Nemeth
authors.affiliation: Department of Anesthesiology and Intensive Therapy, University of Debrecen, Hungary.
authors.forename: Mariann
authors.initials: M
authors.lastname: Berhes
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, University of Debrecen, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kiss
authors.affiliation: Department of Anesthesiology and Intensive Therapy, University of Debrecen, Hungary.
authors.forename: Endre
authors.initials: E
authors.lastname: Hajdu
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, University of Debrecen, Hungary.
authors.forename: Adam
authors.initials: A
authors.lastname: Deak
authors.affiliation: Department of Operative Techniques and Surgical Research, Institute of Surgery, University of Debrecen, Hungary.
authors.forename: Abel
authors.initials: A
authors.lastname: Molnar
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Debrecen, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szabo
authors.affiliation: Department of Anesthesiology and Intensive Therapy, University of Debrecen, Hungary.
authors.forename: Bela
authors.initials: B
authors.lastname: Fulesdi
mesh_terms: D000818:Animals; D002404:Catheterization; D004903:Erythrocyte Aggregation; D004907:Erythrocyte Deformability; D004909:Erythrocyte Indices; D004926:Escherichia coli; D007249:Inflammation / Q000097:blood*; D008833:Microcirculation; D012212:Rheology; D018805:Sepsis; D013552:Swine
keywords: E. coli; Red blood cell deformability; animal model; red blood cell aggregation; sepsis