title: Morphological and molecular analysis of the genus Culicoides (Diptera: Ceratopogonidae) in Slovakia with five new records.
abstract: The biodiversity of Culicoides from eastern Slovakia was investigated by light trapping. An integrative taxonomy approach combining DNA barcode sequence and morphological analyses was used to accurately identify specimens. Five species were newly recorded from Slovakia: Culicoides picturatus Kremer & Deduit, C. gejgelensis Dzhafarov, C. clastrieri Callot et al., C. griseidorsum Kieffer and C. odiatus Austen. The checklist of the Culicoides species recorded from SK has been updated to 63 species and barcode sequence data is provided for 8 species not previously available on GenBank. Conflict between results from molecular and morphological analyses resulted in the discovery of some potentially new cryptic species and the inability of DNA barcodes to distinguish C. festivipennis Kieffer from C. clastrieri, C. salinarius Kieffer from C. manchuriensis Tokunaga and C. pallidicornis Kieffer from C. subfasciipennis Kieffer. These conflicts suggest further study is required to clarify the status of these species.
authors.affiliation: University of Veterinary Medicine and Pharmacy in Košice, Dept. of Parasitology, Komenského 73, SK-04181 Košice, Slovak Republic.; Email: Alica.Kocisova@uvlf.sk.
authors.forename: Adela
authors.initials: A
authors.lastname: Sarvašová
authors.affiliation: University of Veterinary Medicine and Pharmacy in Košice, Dept. of Parasitology, Komenského 73, SK-04181 Košice, Slovak Republic.; Email: unknown.
authors.forename: Alica
authors.initials: A
authors.lastname: Kočišová
authors.affiliation: University of Veterinary Medicine and Pharmacy in Košice, Dept. of Parasitology, Komenského 73, SK-04181 Košice, Slovak Republic.; Email: unknown.
authors.forename: Miloš
authors.initials: M
authors.lastname: Halán
authors.affiliation: Institute of Parasitology and Tropical Pathology (IPPTS), Medicine faculty, EA7292, 3 rue Koeberlé, F-67000 Strasbourg, France.; Email: bmathieu@unistra.fr.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Delécolle
authors.affiliation: Institute of Parasitology and Tropical Pathology (IPPTS), Medicine faculty, EA7292, 3 rue Koeberlé, F-67000 Strasbourg, France. EID Méditerranée, 165 av Paul Rimbaud, F-34184 Montpellier, France; Email: unknown.
authors.forename: Bruno
authors.initials: B
authors.lastname: Mathieu
mesh_terms: D063147:Animal Distribution; D000818:Animals; D049628:Body Size; D002519:Ceratopogonidae / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics* / Q000254:growth & development; D017753:Ecosystem; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D009929:Organ Size; D010802:Phylogeny; D018154:Slovakia; D014921:Wings, Animal / Q000033:anatomy & histology / Q000254:growth & development