title: Purification of human induced pluripotent stem cell-derived neural precursors using magnetic activated cell sorting.
abstract: Neural precursor (NP) cells derived from human induced pluripotent stem cells (hiPSCs), and their neuronal progeny, will play an important role in disease modeling, drug screening tests, central nervous system development studies, and may even become valuable for regenerative medicine treatments. Nonetheless, it is challenging to obtain homogeneous and synchronously differentiated NP populations from hiPSCs, and after neural commitment many pluripotent stem cells remain in the differentiated cultures. Here, we describe an efficient and simple protocol to differentiate hiPSC-derived NPs in 12 days, and we include a final purification stage where Tra-1-60+ pluripotent stem cells (PSCs) are removed using magnetic activated cell sorting (MACS), leaving the NP population nearly free of PSCs.
authors.affiliation: Department of Bioengineering and IBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Avenida Rovisco Pais, 1049-001, Lisbon, Portugal.
authors.forename: Gonçalo M C
authors.initials: GM
authors.lastname: Rodrigues
authors.forename: Tiago G
authors.initials: TG
authors.lastname: Fernandes
authors.forename: Carlos A V
authors.initials: CA
authors.lastname: Rodrigues
authors.forename: Joaquim M S
authors.initials: JM
authors.lastname: Cabral
authors.forename: Maria Margarida
authors.initials: MM
authors.lastname: Diogo
mesh_terms: D018929:Cell Culture Techniques; D002454:Cell Differentiation; D015925:Cryopreservation / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism