title: Osmotic surveillance mediates rapid wound closure through nucleotide release.
abstract: Osmotic cues from the environment mediate rapid detection of epithelial breaches by leukocytes in larval zebrafish tail fins. Using intravital luminescence and fluorescence microscopy, we now show that osmolarity differences between the interstitial fluid and the external environment trigger ATP release at tail fin wounds to initiate rapid wound closure through long-range activation of basal epithelial cell motility. Extracellular nucleotide breakdown, at least in part mediated by ecto-nucleoside triphosphate diphosphohydrolase 3 (Entpd3), restricts the range and duration of osmotically induced cell migration after injury. Thus, in zebrafish larvae, wound repair is driven by an autoregulatory circuit that generates pro-migratory tissue signals as a function of environmental exposure of the inside of the tissue.
authors.affiliation: Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065.
authors.forename: William J
authors.initials: WJ
authors.lastname: Gault
authors.affiliation: Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065.
authors.forename: Balázs
authors.initials: B
authors.lastname: Enyedi
authors.affiliation: Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065 niethamp@mskcc.org.
authors.forename: Philipp
authors.initials: P
authors.lastname: Niethammer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002465:Cell Movement; D004817:Epidermis / Q000503:physiopathology; D004847:Epithelial Cells / Q000502:physiology; D045604:Extracellular Fluid / Q000502:physiology; D006868:Hydrolysis; D007814:Larva; D064587:Osmoregulation; D014945:Wound Healing; D015027:Zebrafish
chemical_list: D000255:Adenosine Triphosphate