title: Polygalacturonase production by calcium alginate immobilized Enterobacter aerogenes NBO2 cells.
abstract: Bacterial cells of Enterobacter aerogenes NBO2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. The optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 10(7) cells/ml) produced 23.48 U/mL of polygalacturonase compared to free cells of 18.54 U/ml. There was about 26.6 % increment in polygalaturonase production. However, in this study, there was 296.6 % of increment in polygalacturonase production after improvement parameters compared to before improvement parameters of calcium alginate bead immobilization cells (5.92 U/ml). This research has indicated that optimized physical parameters of calcium alginate bead immobilization cells have significantly enhanced the production of polygalacturonase.
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Darah
authors.forename: M
authors.initials: M
authors.lastname: Nisha
authors.forename: Sheh-Hong
authors.initials: SH
authors.lastname: Lim
mesh_terms: D000464:Alginates / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis*; D021902:Enterobacter aerogenes / Q000737:chemistry* / Q000201:enzymology*; D005285:Fermentation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D011096:Polygalacturonase / Q000096:biosynthesis*
chemical_list: D000464:Alginates; D001426:Bacterial Proteins; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D011096:Polygalacturonase