title: Near-infrared fluorescence imaging-guided surgery improves recurrence-free survival rate in novel orthotopic animal model of head and neck squamous cell carcinoma.
abstract: Appropriate animal models are required to test novel therapeutics for head and neck squamous cell carcinoma (HNSCC) such as near-infrared (NIR) imaging-guided surgery.
  
  
  
  We developed an optimized animal model of orthotopic HNSCC (in female athymic NMRI (Naval Medical Research Institute) nude mice) with a prolonged survival time. Resection of the orthotopic tumors was performed 30 days after implantation with or without the aid of a miniaturized clinical grade NIR optical imaging device, after systemic administration of a fluorescent RGD-based probe that targets αv β3 integrin.
  
  
  
  NIR optical imaging-guided surgery increased the recurrence-free survival rate by 50% through the detection of fluorescent cancer residues as small as 185 µm; these fragments could remain unidentified if resection was performed exclusively under unaided visual guidance.
  
  
  
  NIR optical imaging-guided surgery showed an improved HNSCC tumor resection quality in our optimized orthotopic animal model. © 2015 Wiley Periodicals, Inc. Head Neck 38: E246-E255, 2016.
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.|Department of Otolaryngology-Head and Neck Surgery, Grenoble University Hospital, Cedex, France.
authors.forename: Ihab
authors.initials: I
authors.lastname: Atallah
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Milet
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Henry
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Josserand
authors.affiliation: Joseph Fourier University, Grenoble Cedex, France.|Department of Otolaryngology-Head and Neck Surgery, Grenoble University Hospital, Cedex, France.
authors.forename: Emile
authors.initials: E
authors.lastname: Reyt
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Coll
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Hurbin
authors.affiliation: INSERM U823, Grenoble Cedex, France.|Joseph Fourier University, Grenoble Cedex, France.|Department of Otolaryngology-Head and Neck Surgery, Grenoble University Hospital, Cedex, France.
authors.forename: Christian Adrien
authors.initials: CA
authors.lastname: Righini
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000601:surgery*; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D006258:Head and Neck Neoplasms / Q000601:surgery*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D061848:Optical Imaging; D025321:Surgery, Computer-Assisted; D015996:Survival Rate
keywords: head and neck squamous cell carcinoma; near-infrared optical imaging-guided surgery; orthotopic animal model; surgical margins; αvβ3 integrin