title: ErbB4 as a potential molecular target in the treatment of esophageal squamous cell cancers.
abstract: ErbB4 is an important member of ErbB subfamily of tyrosine kinases receptor with overexpression in several tumors; however its biological role in esophageal cancer is poorly understood till date. The main objective of this study was to examine whether miRNA-140-5p could target and control ErbB4 expression at transcriptional level. The ErbB4 expressions in different cell lines were evaluated by western blotting and luciferase assay. Moreover, cell proliferation, apoptosis, and cell invasion studies were investigated using MTT, flow cytometry, and transwell assays. miRNA-140-5p remarkably downregulated the ErbB4 expression in EC9706 and TE-1A cell lines. Furthermore, miRNA-140-5p transfected cell significantly controlled the cell proliferation and enhanced the apoptosis of multiple cells. Additionally, miRNA-140-5p had marked effect on the DNA synthesis and caspase 3/7 activity in comparison to control cells. Specifically, miRNA-140-5p inhibited/repressed the cancer cell invasion and migration in a sign to have important biological role in esophageal carcinomas. Taken together, miRNA-140-5p could act as a potential molecular target in ErbB4 overexpressing ESCC cell lines paving the way for effective esophageal cancer treatment.
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Jiang'an District, Wuhan, Hubei 430014, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Jiang'an District, Wuhan, Hubei 430014, China.
authors.forename: Bao-Jun
authors.identifier: 0000-0003-2897-1880
authors.initials: BJ
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, The Central Hospital of Wuhan, Jiang'an District, Wuhan, Hubei 430014, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000628:therapy*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D018307:Neoplasms, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D066247:Receptor, ErbB-4 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D035683:MicroRNAs; C544302:Mirn140 microRNA, human; D012334:RNA, Neoplasm; C587689:ERBB4 protein, human; D066247:Receptor, ErbB-4; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7