title: An UPLC-MS-based metabolomics investigation on the anti-fatigue effect of salidroside in mice.
abstract: An ultra-performance liquid chromatography-quadrupole time-of-flight-based metabolomic approach was developed to study influence of salidroside, an anti-fatigue ingredient from Rhoiola rosea, on urinary metabolic profiling of rats to a single dose of 180 mg/kg per day. Unsupervised principal component analysis (PCA) and supervised orthogonal pre-projection to latent structures discriminate analysis (OPLS-DA) on metabolite profiling revealed obvious differentiation between the salidroside treated groups and controls in both positive and negative ion modes. Eleven urinary metabolites contributing to the differentiation were identified as anti-fatigue biomarkers: N-acetylserotonin, 2-Methoxyestrone 3-glucuronide, Taurine, Melatonin, Sorbitol, Geranyl diphosphate, Z-nucleotide, Cortisone, Dihydrocortisol, Sebacic acid, Pregnenolone sulfate. The physiological significance of these biomarkers is discussed. The work showed that metabolomics is a powerful tool in studying the anti-fatigue effects of natural compound salidroside on multiple targets in vivo.
authors.affiliation: State Key Laboratory of Food Science and Technology (Jiangnan University), School of Food Science and Technology, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Chaoyang
authors.initials: C
authors.lastname: Ma
authors.affiliation: Air Force Logistics College, Xuzhou, Jiangshu 221006, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Food Science and Technology (Jiangnan University), School of Food Science and Technology, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Food Science and Technology (Jiangnan University), School of Food Science and Technology, 1800 Lihu Road, 214122 Wuxi, China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Food Science and Technology (Jiangnan University), School of Food Science and Technology, 1800 Lihu Road, 214122 Wuxi, China. Electronic address: wanghongxin2009@hotmail.com.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005221:Fatigue / Q000188:drug therapy* / Q000652:urine; D005960:Glucosides / Q000302:isolation & purification / Q000652:urine*; D008297:Male; D055432:Metabolomics / Q000295:instrumentation / Q000379:methods*; D008815:Mice, Inbred Strains; D015999:Multivariate Analysis; D010636:Phenols / Q000302:isolation & purification / Q000652:urine*; D025341:Principal Component Analysis; D051381:Rats; D029771:Rhodiola / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013550:Swimming
chemical_list: D015415:Biomarkers; D005960:Glucosides; D010636:Phenols; C009172:rhodioloside
keywords: Anti-fatigue; Biomarkers; Metabolomics; Salidroside; UPLC-MS/MS