title: Synthetic polymers are more effective than natural flocculants for the clarification of tobacco leaf extracts.
abstract: The use of synthetic polymers as flocculants can increase filter capacity and thus reduce the costs of downstream processing during the production of plant-derived biopharmaceutical proteins, but this may also attract regulatory scrutiny due to the potential toxicity of such compounds. Therefore, we investigated the efficacy of three non-toxic natural flocculants (chitosan, kaolin and polyphosphate) alone and in combination with each other or with a synthetic polymer (Polymin P) during the clarification of tobacco leaf extracts. We used a design-of-experiments approach to determine the impact of each combination on filter capacity. We found that Polymin P was most effective when used on its own but the natural flocculants were more effective when used in combination. The combination of chitosan and polyphosphate was the most effective natural flocculant, and this was identified as a potential replacement for Polymin P under neutral and acidic extraction conditions independent of the conductivity, even though the efficiency of flocculation was lower than for Polymin P. None of the tested flocculants reduced the concentration of total soluble protein in the feed stream or the recovery of the model fluorescent protein DsRed.
authors.affiliation: Institute for Molecular Biotechnology, Worringerweg 1, RWTH Aachen University, 52074 Aachen, Germany. Electronic address: johannes.buyel@rwth-aachen.de.
authors.forename: Johannes F
authors.initials: JF
authors.lastname: Buyel
authors.affiliation: Institute for Molecular Biotechnology, Worringerweg 1, RWTH Aachen University, 52074 Aachen, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology, Forckenbeckstraße 6, 52074 Aachen, Germany. Electronic address: fischer@molbiotech.rwth-aachen.de.
authors.forename: Rainer
authors.initials: R
authors.lastname: Fischer
mesh_terms: D005427:Flocculation; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D012107:Research Design; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011108:Polymers; D011994:Recombinant Proteins
keywords: Bioprocess development; Design of experiments (DoE); Plant extract flocculation; Plant-derived biopharmaceuticals; Protein purification