title: miR-155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro.
abstract: The critical role of microRNAs in cancer development has been extensively described. miRNAs are both specific markers and putative therapy targets. miR-155 has been identified to be an oncomiRNA and is highly expressed in several solid cancers, including glioblastoma. In this study, we found that miR-155 is a good potential therapy target. Knockdown of miR-155 sensitizes glioma cells to the chemotherapy of temozolomide (TMZ) by targeting the p38 isoforms mitogen-activated protein kinase 13 [MAPK13, also known as p38 MAPKδ or stress-activated protein kinase 4 (SAPK4)] and MAPK14 (also known as p38 MAPKα). As tumor suppressor genes, MAPK13 and MAPK14 play important roles in lowering the accumulation of reactive oxygen species (ROS), inducing cell apoptosis, and slowing the progression of cancer. Knockdown of miR-155 enhanced the anticancer effect of TMZ on glioma by targeting the MAPK13 and MAPK14-mediated oxidative stress and apoptosis, but did not affect the secretion of MMP2 and MMP9.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.|Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Ran
authors.initials: R
authors.lastname: Zou
authors.affiliation: Xiangya Medical School, Central South University, Changsha, Hunan, PR China.
authors.forename: Rouxi
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Xiangya Medical School, Central South University, Changsha, Hunan, PR China.
authors.forename: Chaofan
authors.initials: C
authors.lastname: Gong
authors.affiliation: Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Chaochao
authors.initials: C
authors.lastname: Tan
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Fang
mesh_terms: D017209:Apoptosis; D001932:Brain Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D048292:Mitogen-Activated Protein Kinase 13 / Q000235:genetics / Q000378:metabolism; D048308:Mitogen-Activated Protein Kinase 14 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D000077204:Temozolomide
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D003606:Dacarbazine; D048292:Mitogen-Activated Protein Kinase 13; D048308:Mitogen-Activated Protein Kinase 14; D000077204:Temozolomide
keywords: CHEMOSENSITIVITY; GLIOBLASTOMA; MAPK; REACTIVE OXYGEN SPECIES; TEMOZOLOMIDE; microRNA