title: Minor Changes in Core Temperature Prior to Cardiac Arrest Influence Outcomes: An Experimental Study.
abstract: OBJECTIVE
  To investigate whether slight variations in core temperature prior to cardiac arrest (CA) influence short-term outcomes and mitochondrial functions.
  
  
  METHODS
  Three groups of New Zealand White rabbits (n = 12/group) were submitted to 15 minutes of CA at 38°C (T-38 group), 39°C (T-39), or 40°C (T 40) and 120 minutes of reperfusion. A Sham-operated group (n = 6) underwent only surgery. Restoration of spontaneous circulation (ROSC), survival, hemodynamics, and pupillary reactivity were recorded. Animals surviving to the end of the observation period were euthanized to assess fresh brain and heart mitochondrial functions (permeability transition and oxidative phosphorylation). Markers of brain and heart damages were also measured.
  
  
  RESULTS
  The duration of asphyxia required to induce CA was significantly lower in the T-40 group when compared to the T-38 group (P < .05). The rate of ROSC was >80% in all groups (P = nonsignificant [ns]). Survival significantly differed among the T-38, T-39, and T-40 groups: 10 (83%) of 12, 7 (58%) of 12, and 4 (33%) of 12, respectively (log-rank test, P = .027). At the end of the protocol, none of the animals in the T-40 group had pupillary reflexes compared to 8 (67%) of 12 in the T-38 group (P < .05). Troponin and protein S100B were significantly higher in the T-40 versus T-38 group (P < .05). Cardiac arrest significantly impaired both inner mitochondrial membrane integrity and oxidative phosphorylation in all groups. Brain mitochondria disorders were significantly more severe in the T-40 group compared to the T-38 group (P < .05).
  
  
  CONCLUSIONS
  Small changes in body temperature prior to asphyxial CA significantly influence brain mitochondrial functions and short-term outcomes in rabbits.
authors.affiliation: Hospices Civils de Lyon, Groupement Hospitalier Edouard Herriot, Service de Réanimation Médicale, Lyon, France Faculté de médecine Lyon-Est, Université de Lyon, Université Claude Bernard Lyon 1, Lyon, France INSERM UMR 1060, CarMeN, Lyon, France.
authors.forename: Martin
authors.initials: M
authors.lastname: Cour
authors.affiliation: Hospices Civils de Lyon, Groupement Hospitalier Edouard Herriot, Service de Réanimation Médicale, Lyon, France Faculté de médecine Lyon-Est, Université de Lyon, Université Claude Bernard Lyon 1, Lyon, France INSERM UMR 1060, CarMeN, Lyon, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Jahandiez
authors.affiliation: INSERM UMR 1060, CarMeN, Lyon, France.
authors.forename: Joseph
authors.initials: J
authors.lastname: Loufouat
authors.affiliation: INSERM UMR 1060, CarMeN, Lyon, France Hospices Civils de Lyon, Groupement Hospitalier Est, Explorations Fonctionnelles Cardiovasculaires & Centre d'Investigations Cliniques de Lyon, Lyon, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Ovize
authors.affiliation: Hospices Civils de Lyon, Groupement Hospitalier Edouard Herriot, Service de Réanimation Médicale, Lyon, France Faculté de médecine Lyon-Est, Université de Lyon, Université Claude Bernard Lyon 1, Lyon, France INSERM UMR 1060, CarMeN, Lyon, France laurent.argaud@chu-lyon.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Argaud
mesh_terms: D000818:Animals; D001237:Asphyxia; D001831:Body Temperature / Q000502:physiology*; D001921:Brain / Q000473:pathology; D004195:Disease Models, Animal; D006323:Heart Arrest / Q000503:physiopathology*; D006439:Hemodynamics / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000473:pathology*; D008930:Mitochondria, Liver / Q000473:pathology*; D051336:Mitochondrial Membranes / Q000378:metabolism; D010085:Oxidative Phosphorylation; D011817:Rabbits
keywords: cardiopulmonary resuscitation; ischemia–reperfusion; mitochondria; mitochondrial permeability transition pore; temperature