title: Enabling technologies to advance microbial isoprenoid production.
abstract: Microbial production of isoprenoids provides an attractive alternative to biomass extraction and chemical synthesis. Although widespread research aims for isoprenoid biosynthesis, it is still in its infancy in terms of delivering commercial products. Large barriers remain in realizing a cost-competitive process, for example, developing an optimal microbial cell factory. Here, we summarize the many tools and methods that have been developed in the metabolic engineering of isoprenoid production, with the advent of systems biology and synthetic biology, and discuss how these technologies advance to accelerate the design-build-test engineering cycle to obtain optimum microbial systems. It is anticipated that innovative combinations of new and existing technologies will continue to emerge, which will enable further development of microbial cell factories for commercial isoprenoid production.
authors.affiliation: Systems and Synthetic Biology, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96, Gothenburg, Sweden.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Yongjin J
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Verena
authors.initials: V
authors.lastname: Siewers
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
mesh_terms: D000465:Algorithms; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D004926:Escherichia coli / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D058615:Synthetic Biology; D049490:Systems Biology; D013729:Terpenes / Q000737:chemistry*
chemical_list: D013729:Terpenes