title: [Efects of Inhibiting miR-155 Expression on the Proliferation and Apoptosis of Leukemia THP-1 Cells].
abstract: The aim of this study was to investigate the effects of miR-155 inhibitor transfection on the proliferation and apoptosis of THP-1 cells. The miR-155 inhibitor was transfected into THP-1 cells (THP-1I) by using X-treme GENE siRNA transfection reagent. Cells without transfection (THP-1C) and cells with negative transfection (THP-1IC) were used as controls. Quantitative real-time polymerase chain reaction (RT-PCR) was performed to detect the expression of miR-155 and relative expression of SHIP1 mRNA in the cells. Cell proliferation was assayed using CCK-8 method. Cell apoptosis were detected by flow cytometry. The expression of SHIP1, TAKT and pAKT in THP-1 cells were detected by Western blot. The results indicated that compared with THP-1C and THP-1IC, the expression of miR-155 in THP-1I cells was significantly reduced; miR-155 inhibition significantly increased apoptosis rate in THP-1 cells (P < 0.05) ; miR-155 inhibition in THP-1 cells caused no significant alteration in SHIP1 mRNA level but significantly increased its protein content, indicating some post-transcriptional modulations might exist underlying the modulation of miR-155 to SHIP1, the miR-155 caused significantly reduced protein level of pAKT (P < 0.05) without interfering TAKT protein content. It is concluded that the miR-155 inhibition may promote THP-1 cell apoptosis through increasing SHIP1 protein content and impairing its downstream PI3K/AKT signaling pathway. This study suggests that miR-155 inhibition may be a promising therapy strategy for treating acute myeloid leukemia (AML).
authors.affiliation: Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China. E-mail: xh-xuehua@163.com.
authors.forename: Hua
authors.initials: H
authors.lastname: Xue
authors.affiliation: Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China.
authors.forename: Hui-Mei
authors.initials: HM
authors.lastname: Guo
authors.affiliation: Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China.
authors.forename: Luo-Ming
authors.initials: LM
authors.lastname: Hua
authors.affiliation: Department of Hematology, Affiliated Hospital of Hebei University, Baoding, Hebei Province 071000, China.
authors.forename: Song-Ying
authors.initials: SY
authors.lastname: Zhao
authors.affiliation: Department of Internal Medicine, Yi County People's Hospital, Baoding 070000, Hebei Province, China.
authors.forename: Hong-Juan
authors.initials: HJ
authors.lastname: Shi
authors.affiliation: Department of Internal Medicine, Yi County People's Hospital, Baoding 070000, Hebei Province, China.
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, Yi County People's Hospital, Baoding 070000, Hebei Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Song
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D019869:Phosphatidylinositol 3-Kinases; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014162:Transfection
chemical_list: C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019869:Phosphatidylinositol 3-Kinases