title: Dietary conjugated linoleic acid and its effect on immune response in pigs infected with the porcine reproductive and respiratory syndrome virus.
abstract: The aim of this study was to evaluate the effects of dietary conjugated linoleic acid (CLA) on immune response in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV). A total of 18 pigs 4 weeks of age were allocated to 3 treatments, 6 per treatment: 0% CLA, 1% CLA, and 2% CLA. Serum IL-1β, IL-6 and TNF-α; lymphocyte proliferation; and IL-2, IFN-γ, IL-10, IL-4 and IL-12 in PBMCs were evaluated. NF-κB, COX2, iNOS and PPAR-γ mRNA were also evaluated. No differences were observed among treatment groups in most of the in vivo cytokine profiles; only TNF-α production was increased in infected pigs in the CLA-supplemented groups. The cytokine profile in vitro was not affected by CLA supplementation. CLA decreased the proliferation of PBMCs stimulated with PRRSVs. Inflammation mediators and PPAR-γ were not affected by CLA in infected pigs. CLA did not improve the immune response of PRRSV infected pigs.
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico. Electronic address: pinelli@ciad.mx.
authors.forename: A
authors.initials: A
authors.lastname: Pinelli-Saavedra
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: J R
authors.initials: JR
authors.lastname: Peralta-Quintana
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Sosa-Castañeda
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: S Y
authors.initials: SY
authors.lastname: Moya-Camarena
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Burgara-Estrella
authors.affiliation: Department of Nutrition and Metabolism, Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD) Apdo. Postal 1735, Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico.
authors.forename: J
authors.initials: J
authors.lastname: Hernández
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology*; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D016328:NF-kappa B / Q000097:blood; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D034421:Sus scrofa / Q000276:immunology*; D013552:Swine; D018417:Th1 Cells / Q000378:metabolism / Q000473:pathology; D018418:Th2 Cells / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D044243:Linoleic Acids, Conjugated; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha
keywords: CLA supplementation; Immune response; PRRSV