title: Antioxidant activities in vitro and in vivo of water-soluble polysaccharide isolated from Sparganium stoloniferum Buch.-Ham.
abstract: In this study, the crude polysaccharides from Sparganium stoloniferum Buch.-Ham were prepared using hot-water extraction and further deproteinzated by Sevage method. The purified fraction of crude polysaccharide was obtained using a DEAE-52 cellulose chromatography and named with WSSP. Then, the antioxidant capacities of WSSP were assessed in vitro and in vivo. The results in vitro indicated that the WSSP possessed notably free radical scavenging capacity. And the antioxidant abilities were dose-dependent and increased with increasing dose of sample. The findings in vivo showed the gavage administration of WSSP can increase SOD and TAOC activities, and decrease MDA levels in tissue and serum of mice. Therefore, the WSSP may serve as a potential antioxidant.
authors.affiliation: Chemical Engineering and Pharmaceutical School, Henan University of Science and Technology, Luoyang, China / Pharmaceutical School, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Pharmaceutical School, Xinxiang Medical University, Xinxiang, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Pharmaceutical School, Xinxiang Medical University, Xinxiang, China.
authors.forename: Qinan
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Pharmaceutical School, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yue
authors.affiliation: Pharmaceutical School, Nanjing University of Chinese Medicine, Nanjing, China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Liang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000097:blood; D004305:Dose-Response Relationship, Drug; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012995:Solubility; D013482:Superoxide Dismutase / Q000097:blood; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water; D008315:Malondialdehyde; D013482:Superoxide Dismutase