title: Integrating an algal β-carotene hydroxylase gene into a designed carotenoid-biosynthesis pathway increases carotenoid production in yeast.
abstract: The algal β-carotene hydroxylase gene Crchyb from Chlamydomonas reinhardtii, Czchyb from Chlorella zofingiensis, or Hpchyb from Haematococcus pluvialis and six other carotenoid-synthesis pathway genes were co-integrated into the genome of a yeast host. Each of these three algal genes showed a higher efficiency to convert β-carotene to downstream carotenoids than the fungal genes from Phaffia rhodozyma. Furthermore, the strain with Hpchyb displayed a higher carotenoid productivity than the strains integrated with Crchyb or Czchyb, indicating that Hpchyb is more efficient than Crchyb and Czchyb. These results suggest that β-carotene hydroxylase plays a crucial role in the biosynthesis of carotenoids.
authors.affiliation: Department of Medical Research, China Medical University Hospital, No. 91 Hsueh-Shih Road, Taichung 402, Taiwan; Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Jui-Jen
authors.initials: JJ
authors.lastname: Chang
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Caroline
authors.initials: C
authors.lastname: Thia
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Hao-Yeh
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Hsien-Lin
authors.initials: HL
authors.lastname: Liu
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Feng-Ju
authors.initials: FJ
authors.lastname: Ho
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Jiunn-Tzong
authors.initials: JT
authors.lastname: Wu
authors.affiliation: Agricultural Biotechnology Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Ming-Che
authors.initials: MC
authors.lastname: Shih
authors.affiliation: Biodiversity Research Center, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Wen-Hsiung
authors.initials: WH
authors.lastname: Li
authors.affiliation: Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Rd, Taichung 402, Taiwan. Electronic address: cchuang@dragon.nchu.edu.tw.
authors.forename: Chieh-Chen
authors.initials: CC
authors.lastname: Huang
mesh_terms: D053898:Biosynthetic Pathways / Q000235:genetics; D002338:Carotenoids / Q000096:biosynthesis*; D000460:Chlorophyta / Q000201:enzymology* / Q000235:genetics*; D002851:Chromatography, High Pressure Liquid; D005818:Genetic Engineering; D006899:Mixed Function Oxygenases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D002338:Carotenoids; D006899:Mixed Function Oxygenases; C079232:beta-carotene hydroxylase
keywords: Carotenoids; Green algae; Synthetic biology; β-Carotene hydroxylase