title: Hct-a is a new actinoporin family from the heteractis crispa sea anemone.
abstract: Several new actinoporin isoforms with molecular weights of 18995.5 to 19398.7 Da exhibiting a high hemolytic activity were isolated from the tropical sea anemone Heteractis crispa using a combination of liquid chromatography techniques. The actinoporins were demonstrated to occur as mono-, di-, and trimers in aqueous solutions. The sequences of the genes encoding actinoporins were identified, and the amino acid sequences of the new polypeptides belonging to the Hct-A actinoporin family were obtained. The new acinoporins differ in their isoelectric points, the number and localization of charged amino acid residues at the functionally important N-terminal fragment of the molecule, as well as in the charge of a tetrapeptide (amino acid residues 74-77) involved in an electrostatic interaction with the cytoplasmic membrane. A recombinant actinoporin, rHct-A2, with a molecular weight of 19141 Da, pI of 9.64, and hemolytic activity of 4.0 × 104 HU/mg, was obtained. The conductivity of the ion channels formed by rHct-A2 in the BLM was demonstrated to be similar to that of the native actinoporin from H. crispa. The obtained data expand knowledge on the structural and functional relationships of actinoporins and contribute to our understanding of the functioning mechanism of these molecules, which is the basis for the development of compounds with a high biomedical potential. Currently, they are considered as models for obtaining antitumor, antibacterial, and cardiac-stimulating agents.
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Leichenko
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Monastirnaya
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Zelepuga
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Tkacheva
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: M P
authors.initials: MP
authors.lastname: Isaeva
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: G N
authors.initials: GN
authors.lastname: Likhatskaya
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: S D
authors.initials: SD
authors.lastname: Anastyuk
authors.affiliation: Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, 100 Let Vladivostoku Prosp., 159, Vladivostok, 690022, Russia.
authors.forename: E P
authors.initials: EP
authors.lastname: Kozlovskaya
keywords: actinoporins; hemolytic activity; lipid membrane conductivity; sea anemone; structural and functional analysis