title: Rapid oriented fibril formation of fish scale collagen facilitates early osteoblastic differentiation of human mesenchymal stem cells.
abstract: We studied the effect of fibril formation of fish scale collagen on the osteoblastic differentiation of human mesenchymal stem cells (hMSCs). We found that hMSCs adhered easily to tilapia scale collagen, which remarkably accelerated the early stage of osteoblastic differentiation in hMSCs during in vitro cell culture. Osteoblastic markers such as ALP activity, osteopontin, and bone morphogenetic protein 2 were markedly upregulated when the hMSCs were cultured on a tilapia collagen surface, especially in the early osteoblastic differentiation stage. We hypothesized that this phenomenon occurs due to specific fibril formation of tilapia collagen. Thus, we examined the time course of collagen fibril formation using high-speed atomic force microscopy. Moreover, to elucidate the effect of the orientation of fibril formation on the differentiation of hMSCs, we measured ALP activity of hMSCs cultured on two types of tilapia scale collagen membranes with different degrees of fibril formation. The ALP activity in hMSCs cultured on a fibrous collagen membrane was significantly higher than on a non-fibrous collagen membrane even before adding osteoblastic differentiation medium. These results showed that the degree of the fibril formation of tilapia collagen was essential for the osteoblastic differentiation of hMSCs.
authors.affiliation: Nanosystem Research Insutitute, AIST (National Institute of Advanced Industrial Science and Technology), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan.
authors.forename: Rena
authors.initials: R
authors.lastname: Matsumoto
authors.affiliation: Nanosystem Research Insutitute, AIST (National Institute of Advanced Industrial Science and Technology), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8562, Japan.
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Uemura
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, S7-6 2-12-1 Ookayama, Meguro-Ku, Tokyo, 152-8550, Japan.
authors.forename: Zhefeng
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Research Department, R & D Division, Taki Chemical Co. Ltd., 346, Miyanishi, Harima-Cho, Kako-Gun, Hyogo, 675-0145, Japan.
authors.forename: Isamu
authors.initials: I
authors.lastname: Yamaguchi
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, S7-6 2-12-1 Ookayama, Meguro-Ku, Tokyo, 152-8550, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ikoma
authors.affiliation: Department of Metallurgy and Ceramics Science, Tokyo Institute of Technology, S7-6 2-12-1 Ookayama, Meguro-Ku, Tokyo, 152-8550, Japan.
authors.forename: Junzo
authors.initials: J
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005399:Fishes; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*
chemical_list: D003094:Collagen
keywords: fibril formation; high-speed atomic force microscopy; human mesenchymal stem cells; osteoblastic differentiation; tilapia scales; type I collagen