title: MD-miniRNA could be a more accurate biomarker for prostate cancer screening compared with serum prostate-specific antigen level.
abstract: Prostate cancer and prostatic hyperplasia detection remains a great challenge, lacking of effective non-invasive and specific diagnostic biomarkers. In the current study, we aimed to identify the relative expression of plasma MD-miniRNA and its diagnostic performance in differentiating prostate cancer and prostatic hyperplasia patients from healthy controls, compared with serum prostate-specific antigen (PSA) level. All of the clinical participants (63 prostate cancer patients, 32 prostatic hyperplasia patients, and 50 healthy controls) were obtained from the Third Affiliated Hospital of Suzhou University in China between January 2013 and April 2014. Clinical characteristics were well matched. Plasma samples were extracted to test the relative expression of MD-miniRNA using the method of qRT-PCR. SPSS 22.0 statistical software package was used to analyze the data and GraphPad Prism 6.0 was used to generate the graphs. Relativity expression of plasma MD-miniRNA was significantly upregulated in prostate cancer, compared with prostatic hyperplasia patients and healthy controls. Serum PSA level revealed similar differences among these groups. MD-miniRNA presented a relatively high diagnostic accuracy with AUC of 0.86 (95 % CI 0.80-0.93) in differentiating prostate cancer patients from healthy controls. Simultaneously, MD-miniRNA was able to discriminate prostate cancer patients from prostatic hyperplasia controls with AUC of 0.79 (95 % CI 0.70-0.88). In addition, MD-miniRNA displayed a better diagnostic performance than PSA level. However, the panel of these two biomarkers revealed the best diagnostic performance, compared with either single biomarker. Results of this study showed that plasma MD-miniRNA could serve as a promising and noninvasive biomarker for diagnosing prostate cancer. Further large-scale studies are needed to confirm its clinical diagnosis accuracy.
authors.affiliation: Department of Urology, Third Affiliated Hospital, Suzhou University, Changzhou, 213003, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Xue
authors.forename: Cui-Xing
authors.initials: CX
authors.lastname: Zhou
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Shi
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: He
mesh_terms: D000368:Aged; D003937:Diagnosis, Differential; D006801:Humans; D008297:Male; D008875:Middle Aged; D017430:Prostate-Specific Antigen / Q000096:biosynthesis / Q000097:blood*; D011470:Prostatic Hyperplasia / Q000097:blood* / Q000235:genetics; D011471:Prostatic Neoplasms / Q000097:blood* / Q000235:genetics; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000097:blood*
chemical_list: C000600023:MD-miniRNA, human; D062085:RNA, Long Noncoding; D017430:Prostate-Specific Antigen