title: Expression and prognostic role of ubiquitination factor E4B in primary hepatocellular carcinoma.
abstract: Ubiquitination factor E4B (UBE4B) has been speculated to have contradictory functions upon tumorigenesis as an oncogene or tumor suppressor in different types of cancers. We investigated the expression and prognostic role of UBE4B in primary hepatocellular carcinoma (HCC) using cell lines and 149 archived HCC samples. Correlation between the functions of UBE4B in HCC was also explored. We used human HCC cell lines (HepG2, Hep3B, SK-Hep1, Huh7, SMMC-7721, BEL-7402) and a normal hepatocyte cell line (LO2) along with HCC samples from patients who had undergone resection for HCC previously at our hospital. A battery of methods (real-time quantitative polymerase chain reaction; Western blotting; immunohistochjemical analyses; cell proliferation and colony formation assays; cell migration and cell invasion assays) were employed to assess various aspects of UBE4B.We found that UBE4B expression was upregulated aberrantly at mRNA and protein levels in human primary HCC tissues. Amplified expression of UBE4B was highly correlated with poor outcome. Silencing of UBE4B expression by siRNA inhibited the proliferation, colony formation, migration and invasion of HCC cells in vitro, and resulted in significant apoptosis that was associated with downregulation of expression of Bcl-2 and upregulation of expression of total p53, p-p53, Bax and Cleaved-Caspase3 in HCC cells. Our findings suggested that UBE4B might have an oncogenic role in human primary HCC, and that it could be used as a prognostic marker (as well as a potential molecular target) for the treatment of HCC.
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Qiu-Zhong
authors.initials: QZ
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Weng
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Qi-Jing
authors.initials: QJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: He
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Chang-Long
authors.initials: CL
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Oncology in Southern China; Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, People's Republic of China.|Department of Biotherapy, Sun Yat-Sen University Cancer Center, Guangzhou, People's Republic of China.
authors.forename: Jian-Chuan
authors.initials: JC
authors.lastname: Xia
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000601:surgery; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality* / Q000601:surgery; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D012307:Risk Factors; D047368:Tumor Burden; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D043743:Ubiquitin-Protein Ligase Complexes / Q000235:genetics* / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases
chemical_list: D051017:Apoptosis Regulatory Proteins; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D043743:Ubiquitin-Protein Ligase Complexes; C473697:UBE4B protein, human; D044767:Ubiquitin-Protein Ligases
keywords: UBE4B; apoptosis; hepatocellular carcinoma; oncogene; prognosis