title: Triptolide (TPL) improves locomotor function recovery in rats and reduces inflammation after spinal cord injury.
abstract: In this study, we studied the effect of triptolide (TPL) on locomotor function in rats with spinal cord injury. A total of 40 rats were studied after dividing them in two major groups, one was experimental group denoted as TPL group while other was control group denoted as PBS group. Each group was subdivided in four subgroups having five rats each (n = 5). TPL was given intraperitonially at the rate of 5 mg/kg/day in TPL group while PBS was given at the same time interval in the same manner in control group for comparison. A reduction in the cavity area of tissue sections was observed by bright field microscopy from 0.22 ± 0.05 to 0.12 ± 0.05 mm(2) in experimental group after 28 days of treatment while BBB score also improved from 1 to 5 after 14 days of treatment. SPSS software, one way ANOVA, was used for recording statistical analysis and values were expressed as mean ± SEM where P value of <0.01 was considered significant. The expression of I-kBα and NF-kB p65 was also studied using western blotting and after recording optical density (OD) values of western blots. It was observed that treatment with TPL significantly reduced the expression of these factors after 28 days of treatment compared with controls.
authors.affiliation: Department of Orthopaedic, The General Hospital of Jinan Military Commanding Region, No. 25 Shifan Road, Tianqiao District, Jinan, 250031, Shandong, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Zhang
authors.forename: Ruo-Xian
authors.initials: RX
authors.lastname: Song
authors.forename: Xiu-Chun
authors.initials: XC
authors.lastname: Yu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004224:Diterpenes / Q000627:therapeutic use*; D004852:Epoxy Compounds / Q000627:therapeutic use; D005260:Female; D020672:I-kappa B Proteins / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology*; D009043:Motor Activity / Q000187:drug effects*; D010616:Phenanthrenes / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects*; D013119:Spinal Cord Injuries / Q000150:complications*; D013997:Time Factors
chemical_list: D004224:Diterpenes; D004852:Epoxy Compounds; C083487:I kappa B beta protein; D020672:I-kappa B Proteins; D010616:Phenanthrenes; C001899:triptolide