title: Ovarian cavity fluid of the viviparous surfperch Neoditrema ransonnetii suppresses the spontaneous cytotoxic activity of head-kidney leucocytes against xenogeneic targets.
abstract: In this study, the effect of ovarian cavity fluid (OCF) from a surfperch Neoditrema ransonnetii on the cytotoxic activity of leucocytes was investigated. In an assay targeting RTG-2, a cell line derived from rainbow trout Oncorhynchus mykiss gonads, leucocytes from both the spleen and head kidney showed spontaneous killing activity. Pre-incubation with OCF significantly suppressed the cytotoxic activity of head-kidney leucocytes towards RTG-2. This suppressive activity was due to the presence of low molecular-mass materials. These results suggest that OCF plays significant roles in pregnancy by its ability to modulate cytotoxicity with maternal leucocytes.
authors.affiliation: School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa 252-0373, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Yokozawa
authors.forename: O
authors.initials: O
authors.lastname: Nakamura
authors.forename: E
authors.initials: E
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Tsutsui
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D060226:Head Kidney / Q000166:cytology*; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology*; D010053:Ovary / Q000737:chemistry*; D010473:Perciformes / Q000276:immunology*; D052286:Viviparity, Nonmammalian
keywords: RTG-2; cytotoxicity; immunosuppression; okitango; viviparity