title: Amphirionin-2, a novel linear polyketide with potent cytotoxic activity from a marine dinoflagellate Amphidinium species.
abstract: A novel linear polyketide, amphirionin-2 (1), with two unique hexahydrofuro[3,2-b]furan moieties has been isolated from the cultivated algal cells of a benthic dinoflagellate Amphidinium sp. (strain KCA09051). The structure was elucidated on the basis of detailed analyses of 2D NMR data, and the absolute configuration of C-5 was determined by using modified Mosher's method. Amphirionin-2 (1) exhibited potent cytotoxic activity against human colon carcinoma Caco-2 cells and human lung adenocarcinoma A549 cells.
authors.affiliation: Science Research Center, Kochi University, Kochi 783-8505, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Kumagai
authors.affiliation: Department of Applied Science, Kochi University, Kochi 783-8502, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Minamida
authors.affiliation: Science Research Center, Kochi University, Kochi 783-8505, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Akakabe
authors.affiliation: Science Research Center, Kochi University, Kochi 783-8505, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tsuda
authors.affiliation: Science Research Center, Kochi University, Kochi 783-8505, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Konishi
authors.affiliation: Graduate School of Kuroshio Science and Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tominaga
authors.affiliation: Center for Advanced Marine Core Research, Kochi University, Kochi 783-8502, Japan. Electronic address: mtsuda@kochi-u.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Tsuda
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Fukushi
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Kawabata
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004141:Dinoflagellida / Q000737:chemistry* / Q000378:metabolism; D005663:Furans / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D061065:Polyketides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D013237:Stereoisomerism
chemical_list: D000199:Actins; D005663:Furans; D061065:Polyketides; C000601195:amphirionin-2
keywords: Amphidinium; Cytotoxic activity; Dinoflagellate; Hexahydrofuro[32-b]furan; Polyketide