title: Nucleotide sequence of conjugative prophage Φ1207.3 (formerly Tn1207.3) carrying the mef(A)/msr(D) genes for eﬄux resistance to macrolides in Streptococcus pyogenes.
abstract: Genetic element Φ1207.3 (formerly Tn1207.3) is a prophage of Streptococcus pyogenes which carries the macrolide eﬄux resistance genes mef(A)/msr(D) and is capable of conjugal transfer among streptococci. Complete nucleotide sequence showed that Φ1207.3 is 52,491 bp in length and contained 58 open reading frames (ORFs). A manual homology-based annotation with functional prediction of the hypothetical gene product was possible only for 34 out of 58 ORFs. Φ1207.3 codes for two different C-methylation systems, several phage structural genes, a lysis cassette (composed by a holin and a peptidoglycan hydrolase), and three site-specific resolvases of the serine recombinase family.
authors.affiliation: Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena Siena, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Iannelli
authors.affiliation: Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania Catania, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Santagati
authors.affiliation: Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena Siena, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Santoro
authors.affiliation: Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena Siena, Italy.
authors.forename: Marco R
authors.initials: MR
authors.lastname: Oggioni
authors.affiliation: Section of Microbiology, Department of Biomedical and Biotechnological Sciences, University of Catania Catania, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Stefani
authors.affiliation: Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena Siena, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Pozzi
keywords: ICE; S. pyogenes; Tn1207.1; Tn1207.3; macrolide resistance; prophage; Φ1207.3