title: Identification of Candida tropicalis BH-6 and synergistic effect with Pantoea agglomerans BH-18 on hydrogen production in marine culture.
abstract: A marine yeast was isolated from mangrove sludge and named Candida tropicalis BH-6. The optimum temperature and the initial pH value for growth of the isolated strain were 37 °C and 5.0, respectively. The strain had high salt tolerance and could survive at NaCl concentrations of 0-6 %. Additionally, the yield of hydrogen production by C. tropicalis BH-6 was only 66.30 ml/l. However, when the yeast was mixed with Pantoea agglomerans BH-18, hydrogen production increased significantly to a maximum of 1707.5 ml/l, which was 36.94 and 247.54 % higher than the monoculture of P. agglomerans BH-18 and C. tropicalis BH-6, respectively. Taken together, these results revealed that in mixed culture, the yeast strain isolated from the same ecosystem as P. agglomerans BH-18 likely consumed the organic acids produced by fermentation, thus eliminating the factor inhibiting hydrogen production by P. agglomerans BH-18. As a result, the yield of hydrogen production during mixed culture increased significantly.
authors.affiliation: College of Marine Science and Engineering, Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
authors.forename: Daling
authors.initials: D
authors.lastname: Zhu
authors.forename: Yingchao
authors.initials: Y
authors.lastname: Ma
authors.forename: Guangce
authors.initials: G
authors.lastname: Wang
authors.forename: Guanghua
authors.initials: G
authors.lastname: Pan
mesh_terms: D041022:Candida tropicalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D008969:Molecular Sequence Data; D020636:Pantoea / Q000378:metabolism*; D012623:Seawater / Q000382:microbiology*; D012722:Sewage / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D012722:Sewage; D012965:Sodium Chloride; D006859:Hydrogen