title: [Kinetics of Cu crossing human erythrocyte membrane].
abstract: This study was aimed to investigate various factors influencing the proceduction of Cu(II) crossing human erythrocyte membrane, including concentration of Cu²⁺, pH value of the medium, temperature and time of incubation, and to derive kinetic equation of Cu(II) crossing human erythrocyte membrane. Suspension red blood cells were incubated by Cu²⁺, then content of Cu²⁺ crossed human erythrocyte membrane was determined by atomic absorption spectrometry under various conditions after digestion. The results showed that content of Cu²⁺ crossed human erythrocyte membrane increased with the increase of extracellular Cu²⁺ and enhancement of incubation temperature, and the content of Cu²⁺ crossed human erythrocyte membrane showed a increasing tendency when pH reached to 6.2-7.4, and to maximum at pH 7.4, then gradually decreased at range of pH 7.4-9.2. It is concluded that the Cu²⁺ crossing human erythrocyte has been confirmed to be the first order kinetics characteristics within 120 min, and the linear equation is 10³ × Y = 0.0497t +6.5992.
authors.affiliation: Tibetan Traditional Medical College, Lasa 850000,Tibetan, China. E-mail: duncila@21cn.com.
authors.forename: Zhu Ci Ren
authors.initials: ZC
authors.lastname: Dun
mesh_terms: D003300:Copper / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013696:Temperature
chemical_list: D003300:Copper