title: Laccase production by Trametes hirsuta, characterization, and its capability of decoloring chlorophyll.
abstract: The present study focused on laccase production, characterization, and its involvement in chlorophyll decolorization. Extracellular laccase, with the highest activity of 11 U/ml on day 8, was efficiently produced from Trametes hirsuta in 5 l bioreactor with optimized media comprising dual carbon sources, glucose and water hyacinth. A laccase was then purified from the supernatant to homogeneity with purification fold of 9.51 and recovery of 39.8% and an estimated molecular mass of 62 kDa by SDS-PAGE. The laccase showed activity at pH 2-6 and temperature 30-80°C and was relatively thermally stable at below 70°C and neutral pH. The laccase was applied to decolorize chlorophyll under different factors: temperature, pH, mediator, metal ions, and enzyme dosage. Other fungal laccases were also found to be able to degrade chlorophyll with rating from 52% to 88% following 1 h treatment with two laccase dosages (5 or 10 U/ml) in the absence of any other mediators at room temperature. These findings may be an important step in developing new, important, and commercially viable industrial applications for laccase enzymes.
authors.forename: Jiayang
authors.initials: J
authors.lastname: Liu
authors.forename: Wenhua
authors.initials: W
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cai
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Huang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liang
mesh_terms: D002734:Chlorophyll / Q000737:chemistry / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics / Q000378:metabolism*; D015394:Molecular Structure; D055454:Trametes / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D002734:Chlorophyll; D042845:Laccase