title: Flow cytometric enumeration of parasitemia in cultures of Plasmodium falciparum stained with SYBR Green I and CD235A.
abstract: A flow cytometric (FACS) detection method for Plasmodium falciparum cultures (P. falciparum) was developed using SYBR Green I and CD235A and compared against the Giemsa stained microscopic examination. The cultured P. falciparum were spiked into red blood cells (RBCs) to yield parasitemia, ranging from 0.01% to 22.0%. FACS analysis demonstrated a clear separation between P. falciparum infected and uninfected RBCs. The measured percentage of parasitemia by FACS revealed higher precision (CV of 2.2-37.2%) with the sensitivity of 0.01% parasitemia than Giemsa stained microscopic examination (CV of 7.2-66.0%). High correlation of measured parasitaemia (r=0.98, P<0.05) was observed between FACS and Giemsa stained microscopic analyses. The higher levels of parasitaemia detection were observed in all ranges by FACS in comparison to Giemsa stained microscopic analysis. The currently reported FACS method using SYBR Green I and CD235A is potentially useful for measuring parasitemia in treating patients.
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jang
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Ju Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Jung
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Yoon
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Chi Hyun
authors.initials: CH
authors.lastname: Cho
authors.affiliation: Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Kangwon-do 200-701, Republic of Korea.
authors.forename: Eun Taek
authors.initials: ET
authors.lastname: Han
authors.affiliation: College of Bionano Technology, Gachon Bionano Research Institute, Kyungwon University, Seongnam-si, Kyeonggi-do 461-701, Republic of Korea.
authors.forename: Seong Soo A
authors.initials: SS
authors.lastname: An
authors.affiliation: Department of Laboratory Medicine, Brain Korea 21 Graduate School of Medicine, College of Medicine, Korea University, Seoul 136-703, Republic of Korea ; Department of Laboratory Medicine, College of Medicine, Korea University, Guro Hospital, Guro 2 Dong, Guro Gu, Seoul 152-703, Republic of Korea.
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
mesh_terms: D001399:Azure Stains; D052160:Benzothiazoles; D003959:Diamines; D004912:Erythrocytes / Q000469:parasitology; D005434:Flow Cytometry / Q000379:methods*; D006021:Glycophorins / Q000378:metabolism*; D006801:Humans; D016778:Malaria, Falciparum / Q000175:diagnosis / Q000469:parasitology; D008853:Microscopy; D009930:Organic Chemicals / Q000378:metabolism*; D018512:Parasitemia / Q000175:diagnosis* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000502:physiology*; D011804:Quinolines; D013194:Staining and Labeling
chemical_list: D001399:Azure Stains; D052160:Benzothiazoles; D003959:Diamines; D006021:Glycophorins; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I