title: Cell therapy for chemically induced ovarian failure in mice.
abstract: Cell therapy has been linked to an unexplained return of ovarian function and fertility in some cancer survivors. Studies modeling this in mice have shown that cells transplantation generates donor-derived oocytes in chemotherapy-treated recipients. This study was conducted to further clarify the impact of cell transplantation from different sources on female reproductive function after chemotherapy using a preclinical mouse model. Methods. Female mice were administered 7.5 mg/kg cisplatin followed by cell transplantation (one week later) using GFP+ female cell donors. For cell tracking, adipose derived stem cell GFP+ (ADSC), female germline stem cell GFP+/MVH+ (FGSC), or ovary cell suspension GFP+ mice were transplanted into cisplatin-treated wild-type recipients. After 7 or 14 days animals were killed and histological analysis, IHQ for GFP cells, and ELISA for estradiol were performed. Results. Histological examinations showed that ADSC, ovary cell suspension, and FGSC transplant increase the number of follicles with apparent normal structure in the cells recipient group euthanized on day 7. Cell tracking showed GFP+ samples 7 days after transplant. Conclusion. These data suggest that intraovarian injection of ADSCs and FGSC into mice with chemotherapy-induced ovarian failure diminished the damage caused by cisplatin.
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGCV/UFRGS, 91540-000 Porto Alegre, RS, Brazil.
authors.forename: Paula
authors.initials: P
authors.lastname: Terraciano
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGCV/UFRGS, 91540-000 Porto Alegre, RS, Brazil.
authors.forename: Tuane
authors.identifier: 0000-0001-7226-5364
authors.initials: T
authors.lastname: Garcez
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGGO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Laura
authors.identifier: 0000-0001-5342-6070
authors.initials: L
authors.lastname: Ayres
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGGO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Isabel
authors.initials: I
authors.lastname: Durli
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGGO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Melchiani
authors.initials: M
authors.lastname: Baggio
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil.
authors.forename: Cristiana Palma
authors.initials: CP
authors.lastname: Kuhl
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Laurino
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGGO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Passos
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGGASTRO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Paz
authors.affiliation: Hospital de Clínicas de Porto Alegre, 90035-903 Porto Alegre, RS, Brazil ; PPGCV/UFRGS, 91540-000 Porto Alegre, RS, Brazil ; PPGGO/UFRGS, 90035-007 Porto Alegre, RS, Brazil.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Cirne-Lima