title: Phagocytic activity of monocytes, their subpopulations and granulocytes during post-transplant adverse events after hematopoietic stem cell transplantation.
abstract: Phagocytosis of granulocytes and monocytes presents a major mechanism that contributes to the clearance of pathogens and cell debris. We analyzed the phagocytic activity of the peripheral blood cell monocytes, three monocyte subpopulations and granulocytes before and up to one year after hematopoietic stem cell transplantation, as well as during transplant-related adverse events. 25 pediatric patients and young adults (median age of 11.0 years) with hemato-oncological malignancies and non malignancies were enrolled in the prospective study. Ingestion of fluorescence-labeled Escherichia coli bacteria was used to assess the phagocytic activity of monocytes and their subpopulations and granulocytes by means of flow cytometry in the patient group as well as in a control group (n=36). During sepsis, a significant increase of phagocytic activity of monocytes (P=0.0003) and a significant decrease of the phagocytic activity of granulocytes (P=0.0003) and the CD14+ CD16++ monocyte subpopulation (P=0.0020) occurred. At the onset of a veno-occlusive disease, a significant increase of phagocytic activity in the CD14++ CD16+ monocyte subpopulation (P=0.001) and a significant decrease in the phagocytic activity of the CD14++ CD16- monocyte subpopulation (P=0.0048) were observed. In conclusion, the phagocytic activity of monocytes, their subpopulations and granulocytes might be a useful and easy determinable parameter that enables identification of post-transplant complications after hematopoietic stem cell transplantation. The alterations of phagocytic activity contribute to the altered immune response that accompanies adverse events after hematopoietic stem cell transplantation.
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany. Electronic address: Michaela.Doering@med.uni-tuebingen.de.
authors.forename: Michaela
authors.initials: M
authors.lastname: Döring
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Karin Melanie
authors.initials: KM
authors.lastname: Cabanillas Stanchi
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Erbacher
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Haufe
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Carl Philipp
authors.initials: CP
authors.lastname: Schwarze
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. I - General Paediatrics, Hematology/Oncology, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Rupert
authors.initials: R
authors.lastname: Handgretinger
authors.affiliation: University Hospital Tuebingen, Children's Hospital, Dpt. III - Paediatric Cardiology, Pulmology, Intensive Care Medicine, Hoppe-Seyler-Str. 1, 72076 Tuebingen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hofbeck
authors.affiliation: University Hospital Gießen, Children's Hospital, Dpt. Paediatric Cardiology, Uhlandstr., 35392 Gießen, Germany.
authors.forename: Gunter
authors.initials: G
authors.lastname: Kerst
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D006098:Granulocytes / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006504:Hepatic Veno-Occlusive Disease / Q000175:diagnosis / Q000209:etiology / Q000276:immunology*; D006801:Humans; D007223:Infant; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D011183:Postoperative Complications / Q000276:immunology*; D011446:Prospective Studies; D017452:Receptors, IgG / Q000378:metabolism; D018805:Sepsis / Q000175:diagnosis / Q000209:etiology / Q000276:immunology*; D055815:Young Adult
chemical_list: D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG
keywords: Hematopoietic stem cell transplantation; Monocyte subpopulations; Monocytes; Pediatric patients; Phagocytic activity; Post-transplant complications