title: Construction of a recombinant lentivirus containing human microRNA-7-3 and its inhibitory effects on glioma proliferation.
abstract: In the present study, we constructed a lentivirus, FIV-CMV-GFP-miR-7-3, containing the microRNA-7-3 gene and the green fluorescent protein gene, and used it to transfect human glioma U251 cells. Fluorescence microscopy showed that 80% of U251 cells expressed green fluorescence. Real-time reverse transcription PCR showed that microRNA-7-3 RNA expression in U251 cells was significantly increased. Proliferation was slowed in transfected U251 cells, and most cells were in the G1 phase of the cell cycle. In addition, the expression of the serine/threonine protein kinase 2 was decreased. Results suggested that transfection with a lentivirus carrying microRNA-7-3 can effectively suppress epidermal growth factor receptor pathway activity in U251 cells, arrest cell cycle transition from G1 phase to S phase and inhibit glioma cell growth.
authors.affiliation: Department of Neurosurgery, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Dong
authors.affiliation: Central Laboratory, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Chongxu
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Hengzhu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Gu
authors.affiliation: Department of Neurosurgery, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Clinical Medical College, Yangzhou University, Yangzhou 225001, Jiangsu Province, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wang
keywords: cell cycle; epidermal growth factor receptor; glioma; lentivirus; microRNA-7-3; neural regeneration; proliferation; serine/threonine protein kinase 2