title: MiR-378 promotes the migration of liver cancer cells by down-regulating Fus expression.
abstract: BACKGROUND
  miR-378 regulates osteoblast differentiation and participates in tumor cell self-renewal and chemo-resistance. However, the function of miR-378 in liver cancer cell migration has not been reported to date.
  
  
  METHODS
  miR-378 expression was examined using real-time quantitative PCR. HepG2 cell migration and liver cell invasion were examined using wound-healing and cell invasion assays. Additionally, HepG2 cell metastasis was analyzed in nude mice.
  
  
  RESULTS
  miR-378 over-expression enhances HepG2 cell proliferation, migration and liver cell invasion. Typical metastatic lesions were found in the livers of mice injected with miR-378-transfected cells, and high levels of the CMV promoter were detected in the nodules, indicating that miR-378 promoted the metastasis of the tumor cells to the liver. We also demonstrated that miR-378 down-regulated Fus expression.
  
  
  CONCLUSIONS
  Our results suggested that miR-378 enhanced cell migration and metastasis by down-regulating Fus expression.
authors.affiliation: Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.
authors.forename: Jichun
authors.initials: J
authors.lastname: Ma
authors.forename: Jiang
authors.initials: J
authors.lastname: Lin
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Qian
authors.forename: Jiayu
authors.initials: J
authors.lastname: Yin
authors.forename: Burton
authors.initials: B
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Tang
authors.forename: Xingxing
authors.initials: X
authors.lastname: Chen
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Wen
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.forename: Zhaoqun
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics; D034702:RNA-Binding Protein FUS / Q000096:biosynthesis* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: C000599550:FUS protein, human; C526012:MIRN378 microRNA, human; D035683:MicroRNAs; D034702:RNA-Binding Protein FUS