title: Elevation in Total Homocysteine Levels in Chinese Patients With Essential Hypertension Treated With Antihypertensive Benazepril.
abstract: OBJECTIVE
  To investigate the effect of benazepril on plasma homocysteine (Hcy) levels and to analyze the correlation between the methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and changes in Hcy levels in response to benazepril.
  
  
  METHODS
  A total of 231 patients with mild to moderate essential hypertension were enrolled, and benazepril was orally administered at a dose of 10 mg/d for 2 weeks. Plasma Hcy levels were measured by high-performance liquid chromatography at baseline and after 2 weeks of treatment. Genotyping of the MTHFR C677T polymorphism was performed by TaqMan probe technique.
  
  
  RESULTS
  There was no significant change in Hcy level after benazepril treatment for 2 weeks (P = .97). However, stratified by baseline Hcy levels, the patients with baseline Hcy <10 μmol/L had a significant increase in plasma Hcy levels (P = .003). The results from the multivariable linear regression analysis demonstrated a significant correlation between baseline Hcy levels and the changes in Hcy levels found in both the unadjusted (P = .002) and the adjusted model (P = .004). Strikingly, we found no significant effect modification by the MTHFR C677T polymorphism on the Hcy changes after benazepril treatment. There were also no statistically significant interactions of gene and environment factors (ie, gene smoking and drinking) on the changes in Hcy levels after benazepril treatment.
  
  
  CONCLUSIONS
  Benazepril may cause an increase in plasma Hcy levels among patients with hypertension with low baseline Hcy levels, while effect modification by MTHFR C677T genotypes on the changes in Hcy levels in response to benazepril was not significant among patients with essential hypertension.
authors.affiliation: School of Life Sciences, Anhui University, Hefei, China Institute of Biomedicine, Anhui Medical University, Hefei, China shanqun@gmail.com.
authors.forename: Shanqun
authors.initials: S
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Mingluo
authors.initials: M
authors.lastname: Pan
authors.affiliation: School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Suwen
authors.initials: S
authors.lastname: Wu
authors.affiliation: Faculty of Health Sciences, Simon Fraser University, Burnaby, British Columbia, Canada.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Venners
authors.affiliation: Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
authors.forename: Guisheng
authors.initials: G
authors.lastname: Zhong
authors.affiliation: Institute for Aging Research, HSL and Harvard Medical School, Boston, MA, USA Molecular and Integrative Physiological Sciences Program, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Yi-Hsiang
authors.initials: YH
authors.lastname: Hsu
authors.affiliation: Department of Statistics, University of Virginia, Charlottesville, VA, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Weinstock
authors.affiliation: Institute of Biomedicine, Anhui Medical University, Hefei, China.
authors.forename: Binyan
authors.initials: B
authors.lastname: Wang
authors.affiliation: Institute of Biomedicine, Anhui Medical University, Hefei, China.
authors.forename: Genfu
authors.initials: G
authors.lastname: Tang
authors.affiliation: School of Life Sciences, Anhui University, Hefei, China.
authors.forename: Dahai
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Biomedicine, Anhui Medical University, Hefei, China Division of Epidemiology and Biostatistics, University of Illinois at Chicago School of Public Health, Chicago, IL, USA.
authors.forename: Xiping
authors.initials: X
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People; D001552:Benzazepines / Q000008:administration & dosage*; D002681:China; D005260:Female; D006710:Homocysteine / Q000097:blood* / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000188:drug therapy* / Q000235:genetics; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D001552:Benzazepines; D006710:Homocysteine; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); C044946:benazepril
keywords: benazepril; essential hypertension; homocysteine; methylenetetrahydrofolate reductase C677T polymorphism