title: Electrospun polyvinyl alcohol/carbon dioxide modified polyethyleneimine composite nanofiber scaffolds.
abstract: A novel biocompatible polyvinyl alcohol/carbon dioxide modified polyethyleneimine (PVA/PEI-CO2) composite nanofiber was fabricated by a green and facile protocol, which reduces the cytotoxicity of PEI through the surface modification of the PEI with CO2. The (13)C NMR spectrum, elemental analysis, and TGA show that CO2 has been incorporated in the PEI surface resulting in a relatively stable structure. The resulting PVA/PEI-CO2 composite nanofibers have been characterized by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), contact angle, and scanning electron microscopy (SEM). The results show that the average diameters of the nanofibers range from 265 ± 53 nm to 423 ± 80 nm. The cytotoxicity of PVA/PEI-CO2 composite nanofibers was assessed by cytotoxicity evaluation using the growth and cell proliferation of normal mice Schwann cells. SEM and the MTT assay demonstrated the promotion of cell growth and proliferation on the PVA/PEI-CO2 composite scaffold. It suggests that PEI-CO2 can have tremendous potential applications in biological material research.
authors.affiliation: Chemical Engineering and Biotechnology, College of Chemistry, Donghua University, Shanghai, China.
authors.forename: Han-Bing
authors.initials: HB
authors.lastname: Wu
authors.affiliation: School of Science, Engineering and Technology, Abertay University, Scotland, UK.
authors.forename: David H
authors.initials: DH
authors.lastname: Bremner
authors.affiliation: Chemical Engineering and Biotechnology, College of Chemistry, Donghua University, Shanghai, China.
authors.forename: Hua-Li
authors.initials: HL
authors.lastname: Nie
authors.affiliation: Chemical Engineering and Biotechnology, College of Chemistry, Donghua University, Shanghai, China jquan@dhu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Quan
authors.affiliation: Chemical Engineering and Biotechnology, College of Chemistry, Donghua University, Shanghai, China lzhu@dhu.edu.cn.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D002245:Carbon Dioxide / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011094:Polyethyleneimine / Q000031:analogs & derivatives* / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry; D051381:Rats; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002245:Carbon Dioxide; D011142:Polyvinyl Alcohol; D011094:Polyethyleneimine
keywords: CO2; Polyethyleneimine derivatives; cell scaffold; composite nanofibers; electrospinning