title: The impact of zinc sulfate addition on the dynamic metabolic profiling of Saccharomyces cerevisiae subjected to long term acetic acid stress treatment and identification of key metabolites involved in the antioxidant effect of zinc.
abstract: The mechanisms of how zinc protects the cells against acetic acid toxicity and acts as an antioxidant are still not clear. Here we present results of the metabolic profiling of the eukaryotic model yeast species Saccharomyces cerevisiae subjected to long term high concentration acetic acid stress treatment in the presence and absence of zinc supplementation. Zinc addition decreased the release of reactive oxygen species (ROS) in the presence of chronic acetic acid stress. The dynamic changes in the accumulation of intermediates in central carbon metabolism were observed, and higher contents of intracellular alanine, valine and serine were observed by zinc supplementation. The most significant change was observed in alanine content, which is 3.51-fold of that of the control culture in cells in the stationary phase. Subsequently, it was found that 0.5 g L(-1) alanine addition resulted in faster glucose consumption in the presence of 5 g L(-1) acetic acid, and apparently decreased ROS accumulation in zinc-supplemented cells. This indicates that alanine exerted its antioxidant effect at least partially through the detoxification of acetic acid. In addition, intracellular glutathione (GSH) accumulation was enhanced by zinc addition, which is related to the protection of yeast cells from the oxidative injury caused by acetic acid. Our studies revealed for the first time that zinc modulates cellular amino acid metabolism and redox balance, especially biosynthesis of alanine and glutathione to exert its antioxidant effect.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China. xqzhao@dlut.edu.cn.
authors.forename: Chun
authors.initials: C
authors.lastname: Wan
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Fang
authors.forename: Liang
authors.initials: L
authors.lastname: Xiong
authors.forename: Xinqing
authors.initials: X
authors.lastname: Zhao
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hasunuma
authors.forename: Fengwu
authors.initials: F
authors.lastname: Bai
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D019342:Acetic Acid / Q000633:toxicity*; D000409:Alanine / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D019149:Bioreactors / Q000382:microbiology; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005453:Fluorescence; D005990:Glycerol / Q000494:pharmacology; D055442:Metabolome / Q000187:drug effects*; D055432:Metabolomics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013312:Stress, Physiological / Q000187:drug effects*; D019802:Succinic Acid / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology*; D019287:Zinc Sulfate / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D000431:Ethanol; D019287:Zinc Sulfate; D019802:Succinic Acid; D015032:Zinc; D000409:Alanine; D005990:Glycerol; D019342:Acetic Acid