title: Proteomic analysis of silkworm midgut cellular proteins interacting with the 5' end of infectious flacherie virus genomic RNA.
abstract: The flacherie disease in the silkworm is caused by the infectious flacherie virus (IFV). IFV relies on its 5' region of genomic RNA to recruit host-related factors to implement viral translation and replication. To identify host proteins bound to the 5'-region of IFV RNA and identify proteins important for its function, mass spectrometry was used to identify proteins from silkworm midgut extracts that were obtained using RNA aptamer-labeled 5' region of IFV RNA. We found 325 protein groups (unique peptide≥2) bound to the 5' region of IFV RNA including translation-related factors (16 ribosomal subunits, 3 eukaryotic initiation factor subunits, 1 elongation factor subunit and 6 potential internal ribosome entry site trans-acting factors), cytoskeleton-related proteins, membrane-related proteins, metabolism enzymes, and other proteins. These results can be used to study the translation and replication related factors of IFV interacting with host silkworm and to control flacherie disease in silkworm.
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Mingqian
authors.initials: M
authors.lastname: Li
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: He
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhangwuke
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiangkang
authors.initials: X
authors.lastname: He
authors.affiliation: Silkworm Pathology and Disease Control Laboratory, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China xmlu@zju.edu.cn.
authors.forename: Xingmeng
authors.initials: X
authors.lastname: Lu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D012831:Bombyx / Q000821:virology*; D003599:Cytoskeleton / Q000737:chemistry; D020869:Gene Expression Profiling; D016679:Genome, Viral; D019476:Insect Proteins / Q000737:chemistry*; D007304:Insect Viruses / Q000235:genetics*; D009690:Nucleic Acid Conformation; D010957:Plasmids; D040901:Proteomics; D012313:RNA / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry*; D012270:Ribosomes / Q000737:chemistry / Q000648:ultrastructure; D053719:Tandem Mass Spectrometry
chemical_list: D052157:Aptamers, Nucleotide; D019476:Insect Proteins; D012367:RNA, Viral; D012313:RNA
keywords: RNA-binding protein; infectious flacherie virus; mass spectrometry; midgut cellular protein; silkworm