title: New methods for determining proton pumping ability and electron transfer activity of the cytochrome bc1 complex.
abstract: It is important to determine the electron transfer activity and proton pumping ability of the cytochrome bc1 complex for better understanding its structure and function. In this study, several methods for determining the electron transfer and proton pumping of the bc1 complex, including the traditional and the new methods, are presented and evaluated. For determining the proton pumping ability of the bc1 complex, the new stopped-flow method has a higher accuracy than the traditional pH meter method, and the new spectrophotometer method is more convenient than the traditional pH meter method. In measuring the electron transfer activity of the bc1 complex, the new stopped-flow method is more accurate and has a higher separating capacity than the traditional spectrophotometer method.
authors.affiliation: College of Life Sciences, Linyi University, Linyi 276000, China Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078, USA quyuangang@lyu.edu.cn.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Life Sciences, Linyi University, Linyi 276000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dong
mesh_terms: D000818:Animals; D002417:Cattle; D045304:Cytochromes c / Q000378:metabolism; D004579:Electron Transport; D014450:Electron Transport Complex III / Q000378:metabolism*; D004583:Electrons; D006736:Horses / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008564:Membrane Potentials; D009154:Mutation; D009206:Myocardium / Q000378:metabolism; D017494:Proton Pumps / Q000378:metabolism*; D016992:Rhodobacter / Q000235:genetics; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D017494:Proton Pumps; D045304:Cytochromes c; D014450:Electron Transport Complex III
keywords: cytochrome bc1 complex; electron transfer; method; proton pumping