title: Structure-based design and synthesis of a bivalent iminobiotin analog showing strong affinity toward a low immunogenic streptavidin mutant.
abstract: The streptavidin/biotin interaction has been widely used as a useful tool in research fields. For application to a pre-targeting system, we previously developed a streptavidin mutant that binds to an iminobiotin analog while abolishing affinity for natural biocytin. Here, we design a bivalent iminobiotin analog that shows 1000-fold higher affinity than before, and determine its crystal structure complexed with the mutant protein.
authors.affiliation: a Division of Applied Chemistry, Graduate School of Engineering , Osaka University , Suita , Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Kawato
authors.forename: Eiichi
authors.initials: E
authors.lastname: Mizohata
authors.forename: Yohei
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Meshizuka
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Noriaki
authors.initials: N
authors.lastname: Takasu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Matsuoka
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Matsumura
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Motomu
authors.initials: M
authors.lastname: Kanai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Doi
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Akira
authors.initials: A
authors.lastname: Sugiyama
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D018360:Crystallography, X-Ray; D015195:Drug Design; D008958:Models, Molecular; D009154:Mutation; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D019809:Streptavidin / Q000737:chemistry*
chemical_list: C518163:iminobiotin; D001710:Biotin; D019809:Streptavidin
keywords: X-ray structural analysis; bivalency; iminobiotin; pre-targeting; streptavidin