title: Recombinant interleukin-1 receptor antagonist attenuates the severity of chronic pancreatitis induced by TNBS in rats.
abstract: Chronic pancreatitis (CP) is a common disease in the department of gastroenterology, with the main symptoms of exocrine and/or endocrine insufficiency and abdominal pain. The pathogenic mechanism of CP is still not fully clarified and the aims of treatment now are to relieve symptoms. In this study, we attempted to find a connection between interleukin-1β (IL-1β) and interleukin-1 receptor antagonist (IL-1Ra) in trinitrobenzene sulfonic acid (TNBS)-induced chronic pancreatitis, and then the therapeutic effect of recombinant IL-1Ra was also detected in the CP model. Chronic pancreatitis was induced by intraductal infusion of TNBS in SD rats followed by a consecutive administration of rIL-1Ra, and the histological changes and collagen content in the pancreas were measured, as well as the abdominal hypersensitivity. We found that rhIL-1Ra could attenuate the severity of chronic pancreatic injury, modulate the extracellular matrix secretion, focal proliferation and apoptosis, and cellular immunity in TNBS-induced CP. Interestingly, rIL-1Ra could also block the pancreatitis-induced referred abdominal hypersensitivity. In conclusion, IL-1Ra may play a protective role in CP and rIL-1Ra would be a potential therapeutic target for the treatment of CP, while its possible mechanisms and clinical usage still need further investigation.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. Electronic address: sjqsz@126.com.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Central Research Institute, Shanghai Pharmaceuticals Holding Co. Ltd., Shanghai 200219, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jia
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: He
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Shanghai Municipality Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Shunying
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Wu
authors.affiliation: Laboratory of Regeneromics, School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
mesh_terms: D000818:Animals; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D050500:Pancreatitis, Chronic / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors*; D011994:Recombinant Proteins / Q000627:therapeutic use; D012720:Severity of Illness Index; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity*
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D014302:Trinitrobenzenesulfonic Acid
keywords: Chronic pancreatitis; Rats; Recombinant interleukin-1 receptor antagonist (rIL-1Ra); Trinitrobenzene sulfonic acid (TNBS)