title: The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model.
abstract: Postoperative regeneration of the middle ear mucosa and pneumatization of the middle ear cavity are of great importance after middle ear surgery. This study developed a new method to transplant autologous nasal mucosal epithelial cell sheets into the damaged middle ear cavity. The aim of this study was to evaluate postoperative healing after the transplantation of the cell sheets. Rabbit nasal mucosal epithelial cell sheets were fabricated on a temperature-responsive culture dish, and transplanted into the damaged middle ear of rabbit, which was surgically created. The healing of middle ears was evaluated by histology and X-ray computed tomography after transplantation. Functional evaluation was performed by measuring the maximum middle ear total pressure reflecting a trans-mucosal gas exchange function. Two control groups were used: the normal control group and the mucosa-eliminated control group. Transplantation of cell sheets suppressed the bone hyperplasia and the narrowing of pneumatic space in the middle ear cavity compared with the mucosa-eliminated control group. The mucosal gas exchange function was also better in the cell sheet-transplanted group. Nasal mucosal epithelial cell sheet was confirmed to be useful as an effective graft material after middle ear surgery and hopefully become a novel therapy in the future.
authors.affiliation: Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, Tokyo, Japan; Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, Japan. Electronic address: kazu1109@jikei.ac.jp.
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Hama
authors.affiliation: Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.affiliation: Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Uchimizu
authors.affiliation: Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Takagi
authors.affiliation: Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Yaguchi
authors.affiliation: Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Okano
authors.affiliation: Department of Oto-Rhino-Laryngology, Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kojima
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004432:Ear, Middle / Q000000981:diagnostic imaging / Q000601:surgery*; D004847:Epithelial Cells / Q000166:cytology / Q000637:transplantation*; D007150:Immunohistochemistry; D009297:Nasal Mucosa / Q000166:cytology*; D011312:Pressure; D011817:Rabbits; D013701:Temporal Bone / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed
keywords: Cell sheet; Cholesteatoma; Middle ear surgery; Mucosal regeneration; Nasal mucosal epithelium; Temperature-responsive culture dish