title: Characterization of bovine serum albumin partitioning behaviors in polymer-salt aqueous two-phase systems.
abstract: In this paper, a linear relationship is proposed relating the natural logarithm of partition coefficient, ln K for protein partitioning in poly (ethylene glycol) (PEG)-phosphate aqueous two-phase system (ATPS) to the square of tie-line length (TLL(2)). This relationship provides good fits (r(2) > 0.98) to the partition of bovine serum albumin (BSA) in PEG (1450 g/mol, 2000 g/mol, 3350 g/mol, and 4000 g/mol)-phosphate ATPS with TLL of 25.0-50.0% (w/w) at pH 7.0. Results also showed that the plot of ln K against pH for BSA partitioning in the ATPS containing 33.0% (w/w) PEG1450 and 8.0% (w/w) phosphate with varied working pH between 6.0 and 9.0 exhibited a linear relationship which is in good agreement (r(2) = 0.94) with the proposed relationship, ln K = α' pH + β'. These results suggested that both the relationships proposed could be applied to correlate and elucidate the partition behavior of biomolecules in the polymer-salt ATPS. The influence of other system parameters on the partition behavior of BSA was also investigated. An optimum BSA yield of 90.80% in the top phase and K of 2.40 was achieved in an ATPS constituted with 33.0% (w/w) PEG 1450 and 8.0% (w/w) phosphate in the presence of 8.5% (w/w) sodium chloride (NaCl) at pH 9.0 for 0.3% (w/w) BSA load.
authors.affiliation: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
authors.forename: Yin Hui
authors.initials: YH
authors.lastname: Chow
authors.affiliation: Department of Applied Mathematics, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
authors.forename: Yee Jiun
authors.initials: YJ
authors.lastname: Yap
authors.affiliation: Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
authors.forename: Chin Ping
authors.initials: CP
authors.lastname: Tan
authors.affiliation: Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
authors.forename: Mohd Shamsul
authors.initials: MS
authors.lastname: Anuar
authors.affiliation: Center for Infectious Diseases Research, Surya University, JI. Scientia Boulevard Block U/7, Gading Serpong, Tangerang 15810, Banten, Indonesia.
authors.forename: Bimo Ario
authors.initials: BA
authors.lastname: Tejo
authors.affiliation: Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia.
authors.forename: Pau Loke
authors.initials: PL
authors.lastname: Show
authors.affiliation: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang 43400, Selangor, Malaysia.
authors.forename: Arbakariya Bin
authors.initials: AB
authors.lastname: Ariff
authors.affiliation: School of Chemical Sciences, Universiti Sains Malaysia, Minden 11800, Malaysia.
authors.forename: Eng-Poh
authors.initials: EP
authors.lastname: Ng
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. Electronic address: tcling@um.edu.my.
authors.forename: Tau Chuan
authors.initials: TC
authors.lastname: Ling
mesh_terms: D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification; D012965:Sodium Chloride / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D010710:Phosphates; D014867:Water; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D012965:Sodium Chloride
keywords: Aqueous two-phase system; Bioseparation; Bovine serum albumin; Protein recovery; Purification; Statistical mechanics