title: Increased production of intestinal immunoglobulins in Syntenin-1-deficient mice.
abstract: Syntenin-1 is an intracellular PDZ protein that binds multiple proteins and regulates protein trafficking, cancer metastasis, exosome production, synaptic formation, and IL-5 signaling. However, the functions of Syntenin-1 have not yet been clearly characterized in detail, especially in vivo. In this study, we generated a Syntenin-1 knock out (KO) mouse strain and analyzed the role(s) of Syntenin-1 in IL-5 signaling, because the direct interaction of Syntenin-1 with the cytoplasmic domain of the IL-5 receptor α subunit and the regulation of IL-5 signaling by Syntenin-1 have been reported. Unexpectedly, the number of IL-5-responding cells was normal and the levels of fecal immunoglobulins were rather higher in the Syntenin-1 KO mice. We also found that IgA and IgM production of splenic B cells stimulated in vitro was increased in Syntenin-1 KO mice. In addition, we showed that a distribution of intestinal microbial flora was influenced in Syntenin-1 KO mice. Our data indicate that Syntenin-1 negatively regulates the intestinal immunoglobulin production and has a function to maintain the intestinal homeostasis in vivo. The analysis of Syntenin-1 KO mice may provide novel information on not only mucosal immunity but also other functions of Syntenin-1 such as cancer metastasis and neural development.
authors.affiliation: Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan; Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Tamura
authors.affiliation: Department of Immunobiology and Pharmacological Genetics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan. Electronic address: mikutani@med.u-toyama.ac.jp.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ikutani
authors.affiliation: Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Yoshida
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Tanaka-Hayashi
authors.affiliation: Department of Immunobiology and Pharmacological Genetics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Yanagibashi
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Ran
authors.initials: R
authors.lastname: Inoue
authors.affiliation: Department of Immunobiology and Pharmacological Genetics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nagai
authors.affiliation: Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Department of Pediatrics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Miyawaki
authors.affiliation: Department of Immunobiology and Pharmacological Genetics, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Takatsu
authors.affiliation: Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0152, Japan. Electronic address: hmori@med.u-toyama.ac.jp.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Mori
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000235:genetics; D000069196:Gastrointestinal Microbiome / Q000235:genetics; D006706:Homeostasis / Q000235:genetics; D018928:Immunity, Mucosal / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000378:metabolism; D007422:Intestines / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D015398:Signal Transduction / Q000235:genetics; D053676:Syntenins / Q000235:genetics / Q000378:metabolism*
chemical_list: D007136:Immunoglobulins; D015848:Interleukin-5; C484513:Sdcbp protein, mouse; D053676:Syntenins
keywords: Antibodies; B cells; Knockout mice; Mucosal immunity; Syntenin-1