title: Immunohistochemical distribution of leptin in kidney tissues of melatonin treated diabetic rats.
abstract: We examined using immunohistochemistry the distribution of leptin in kidney tissues of melatonin treated, streptozotocin (STZ) diabetic rats. The animals were divided into five groups: control, sham, melatonin-treated, diabetic and melatonin-treated diabetic. Kidney sections were prepared and stained with hematoxylin and eosin, and Crossman's triple staining for histological examination. The immunohistochemical localization of leptin in the kidney tissue was determined using the streptavidin-biotin-peroxidase method. We determined that on days 7 and 14, the leptin immunoreactivity of the diabetic and melatonin-treated diabetic groups was weaker than for the other groups. Weak immunoreactivity was found in the proximal and distal tubules of the kidney in the diabetic and melatonin-treated diabetic groups on days 7 and 14, and strong immunoreactivity was found in the control, sham and melatonin groups. Melatonin application had no significant effect on leptin production in the kidney tissues of diabetic rats.
authors.affiliation: School of Health Sciences, Kafkas University , Kars.
authors.forename: S
authors.initials: S
authors.lastname: Elis Yildiz
authors.forename: T
authors.initials: T
authors.lastname: Deprem
authors.forename: E
authors.initials: E
authors.lastname: Karadag Sari
authors.forename: S A
authors.initials: SA
authors.lastname: Bingol
authors.forename: S
authors.initials: S
authors.lastname: Koral Tasci
authors.forename: S
authors.initials: S
authors.lastname: Aslan
authors.forename: G
authors.initials: G
authors.lastname: Nur
authors.forename: M
authors.initials: M
authors.lastname: Sozmen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced* / Q000188:drug therapy*; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism*; D020738:Leptin / Q000378:metabolism*; D008550:Melatonin / Q000627:therapeutic use*; D009929:Organ Size; D051381:Rats; D013311:Streptozocin
chemical_list: D000975:Antioxidants; D020738:Leptin; D013311:Streptozocin; D008550:Melatonin
keywords: diabetes mellitus; immunohistochemistry; kidney; leptin; melatonin