title: Human adipose-derived stem cells attenuate inflammatory bowel disease in IL-10 knockout mice.
abstract: Inflammatory bowel disease (IBD) is a complex immunological disorder characterized by chronic inflammation caused mainly by unknown factors. The interleukin-10 knockout (IL-10 KO) mouse is a well-established murine model of IBD which develops spontaneous intestinal inflammation that resembles Crohn's disease. In the present study, human adipose-derived mesenchymal stem cells (hAMSCs) were administrated to IL-10 KO mice to evaluate the anti-inflammatory effects of hAMSCs that may attenuate the progress of or treat IBD. After IBD was induced by feeding the IL-10 KO mouse a 125-250 ppm piroxicam mixed diet for 1 week, 2×10(6) hAMSCs were injected into the peritoneum and the mice were switched to a normal diet. After 1 week, the mice were sacrificed and tissue samples were harvested. Tissue scores for inflammation and inflammation-related genes expression were determined. The hAMSC-treated group showed significantly reduced inflammatory changes in histological analysis. Reverse transcription-PCR analysis showed that RANTES, Toll-like receptor 9, and IL-4 expression levels were not significantly different between the groups while IL-12, INF-γ, and TNF-α levels were significantly decreased in the hAMSC treated group. hAMSC attenuated IBD in the IL-10 KO mice by suppressing inflammatory cytokine expression, was mediated by the type 1 helper T cell pathway. Even though only a single injection of hAMSCs was delivered, the effect influenced chronic events associated with inflammatory changes and demonstrated that hAMSCs are a powerful candidate for IBD therapy.
authors.affiliation: Department of Anatomy, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea.
authors.forename: Woo Yeun
authors.initials: WY
authors.lastname: Jung
authors.affiliation: Department of Anatomy, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea.
authors.forename: Joo Hwan
authors.initials: JH
authors.lastname: Kang
authors.affiliation: Department of Anatomy, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea.
authors.forename: Kyung Gon
authors.initials: KG
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea.
authors.forename: Hee Snn
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea.
authors.forename: Byung Ik
authors.initials: BI
authors.lastname: Jang
authors.affiliation: Department of Pediatrics, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea. Electronic address: yhpark@med.yu.ac.kr.
authors.forename: Yong Hoon
authors.initials: YH
authors.lastname: Park
authors.affiliation: Department of Anatomy, Yeungnam University College of Medicine, 170, Hyeonchung-ro, Nam-gu, Daegu, 705-717, Republic of Korea. Electronic address: ihsong@med.yu.ac.kr.
authors.forename: In-Hwan
authors.initials: IH
authors.lastname: Song
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000637:transplantation; D000818:Animals; D064987:Cell- and Tissue-Based Therapy; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D015212:Inflammatory Bowel Diseases / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D016753:Interleukin-10 / Q000235:genetics*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D018345:Mice, Knockout; D010894:Piroxicam / Q000008:administration & dosage; D051217:Toll-Like Receptor 9 / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D016207:Cytokines; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D010894:Piroxicam
keywords: Adipose tissue; Inflammatory bowel disease; Interleukin-10; Knockout mice; Mesenchymal stem cells