title: The notochord breaks bilateral symmetry by controlling cell shapes in the zebrafish laterality organ.
abstract: Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Julien
authors.initials: J
authors.lastname: Compagnon
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Barone
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Srivarsha
authors.initials: S
authors.lastname: Rajshekar
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Rita
authors.initials: R
authors.lastname: Kottmeier
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Kornelija
authors.initials: K
authors.lastname: Pranjic-Ferscha
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Behrndt
authors.affiliation: Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria. Electronic address: heisenberg@ist.ac.at.
authors.forename: Carl-Philipp
authors.initials: CP
authors.lastname: Heisenberg
mesh_terms: D000818:Animals; D019521:Body Patterning; D002467:Cell Nucleus / Q000378:metabolism; D048430:Cell Shape; D002923:Cilia / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology; D004848:Epithelium / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D009672:Notochord / Q000196:embryology* / Q000378:metabolism; D019170:Somites / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins