title: CEP2 attenuates myoblast differentiation but does not affect proliferation.
abstract: CEP2 (CDC42EP2) is a member of the CDC42 subfamily that belongs to the Rho family. The Rho family plays an important role in a variety of cellular processes including skeletal myogenesis. Here, we find the expression of CEP2 increased significantly during C2C12 myogenesis. Overexpression of CEP2 could attenuate myoblast differentiation, while knockdown of CEP2 by siRNA results in enhancing myogenesis. Furthermore, we demonstrate for the first time that CEP2 attenuates myoblast differentiation via suppression of muscle regulatory factors (MRFs) rather than influencing myoblast proliferation. These results indicate that CEP2 acts as a repressor during myogenesis, which provides new insights into the role of CEP2 in muscle development.
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Ming
authors.initials: M
authors.lastname: Wu
authors.affiliation: 2. Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors.forename: Gongshe
authors.initials: G
authors.lastname: Yang
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Xingyu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Hu
authors.initials: H
authors.lastname: Chen
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Mingsen
authors.initials: M
authors.lastname: Li
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Kaifan
authors.initials: K
authors.lastname: Yu
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Xumeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Shuihua
authors.initials: S
authors.lastname: Xie
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: 2. Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
authors.forename: Guiyan
authors.initials: G
authors.lastname: Chu
authors.affiliation: 1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510006, China;
authors.forename: Delin
authors.initials: D
authors.lastname: Mo
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D032446:Myoblasts / Q000378:metabolism / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D064067:Rho Guanine Nucleotide Exchange Factors / Q000378:metabolism*
chemical_list: C000601180:Cep250 protein, mouse; D018797:Cell Cycle Proteins; D064067:Rho Guanine Nucleotide Exchange Factors
keywords: CEP2; myoblast proliferation.; myogenesis; skeletal muscle