title: Biodegradable hydrogels composed of oxime crosslinked poly(ethylene glycol), hyaluronic acid and collagen: a tunable platform for soft tissue engineering.
abstract: In situ crosslinking hydrogels are attractive for application as injectable hydrogel-based tissue scaffolds that adapt to fill patient-specific cavities. Oxime click chemistry was used to crosslink hydrogels that were biodegradable, soft and supportive of cell adhesion. Linear poly(ethylene glycol)s (PEGs, Mn 2 or 4 kDa) terminated at both ends with aminooxy moieties and hyaluronic acid (HA, Mn 2 MDa) derivatives displaying aldehydes were non-toxic towards primary Schwann cells. The PEG and HA derivatives form oxime crosslinked hydrogels with mechanical and swelling properties that were tunable based on the composition of the hydrogels to values analogous to soft tissues such as those found in the central or peripheral nervous system. Gels incorporating collagen-1 supported the adhesion of human mesenchymal stem cells. Such chemistry has the potential to generate clinically relevant injectable hydrogels for minimally invasive personalized medical procedures in the central or peripheral nervous systems.
authors.affiliation: a J. Crayton Pruitt Family Department of Biomedical Engineering , University of Florida , Biomedical Sciences Building JG-53, P.O. Box 116131, Gainesville , FL 32611-6131 , USA.
authors.forename: John G
authors.initials: JG
authors.lastname: Hardy
authors.forename: Phillip
authors.initials: P
authors.lastname: Lin
authors.forename: Christine E
authors.initials: CE
authors.lastname: Schmidt
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003094:Collagen / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry*; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000502:physiology; D015394:Molecular Structure; D010091:Oximes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects / Q000502:physiology; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000295:instrumentation; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels; D010091:Oximes; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D003094:Collagen
keywords: biodegradable; biomaterials; biomimetic mechanical properties; central nervous system; in vitro cell culture; injectable hydrogels; peripheral nervous system; tissue engineering