title: Complete genome sequence analysis of Pseudomonas aeruginosa N002 reveals its genetic adaptation for crude oil degradation.
abstract: The present research work reports the whole genome sequence analysis of Pseudomonas aeruginosa strain N002 isolated from crude oil contaminated soil of Assam, India having high crude oil degradation ability. The whole genome of the strain N002 was sequenced by shotgun sequencing using Ion Torrent method and complete genome sequence analysis was done. It was found that the strain N002 revealed versatility for degradation, emulsification and metabolizing of crude oil. Analysis of cluster of orthologous group (COG) revealed that N002 has significantly higher gene abundance for cell motility, lipid transport and metabolism, intracellular trafficking, secretion and vesicular transport, secondary metabolite biosynthesis, transport and catabolism, signal transduction mechanism and transcription than average levels found in other genome sequences of the same bacterial species. However, lower gene abundance for carbohydrate transport and metabolism, replication, recombination and repair, translation, ribosomal structure, biogenesis was observed in N002 than average levels of other bacterial species.
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Dhrubajyoti
authors.initials: D
authors.lastname: Das
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Reshita
authors.initials: R
authors.lastname: Baruah
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Sarma Roy
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India. Electronic address: 1010anil@gmail.com.
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India. Electronic address: dekaboruah@yahoo.com.
authors.forename: Hari Prasanna
authors.initials: HP
authors.lastname: Deka Boruah
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Jatin
authors.initials: J
authors.lastname: Kalita
authors.affiliation: Department of Biotechnology, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India.
authors.forename: Tarun Chandra
authors.initials: TC
authors.lastname: Bora
mesh_terms: D001483:Base Sequence; D030561:Databases, Nucleic Acid; D016680:Genome, Bacterial; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008969:Molecular Sequence Data; D010578:Petroleum / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D010578:Petroleum
keywords: Bioremediation; Crude oil; Genome; Pseudomonas aeruginosa