title: Transcriptional responses of a bicarbonate-tolerant monocot, Puccinellia tenuiflora, and a related bicarbonate-sensitive species, Poa annua, to NaHCO3 stress.
abstract: Puccinellia tenuiflora is an alkaline salt-tolerant monocot found in saline-alkali soil in China. To identify the genes which are determining the higher tolerance of P. tenuiflora compared to bicarbonate sensitive species, we examined the responses of P. tenuiflora and a related bicarbonate-sensitive Poeae plant, Poa annua, to two days of 20 mM NaHCO3 stress by RNA-seq analysis. We obtained 28 and 38 million reads for P. tenuiflora and P. annua, respectively. For each species, the reads of both unstressed and stressed samples were combined for de novo assembly of contigs. We obtained 77,329 contigs for P. tenuiflora and 115,335 contigs for P. annua. NaHCO3 stress resulted in greater than two-fold absolute expression value changes in 157 of the P. tenuiflora contigs and 1090 of P. annua contigs. Homologs of the genes involved in Fe acquisition, which are important for the survival of plants under alkaline stress, were up-regulated in P. tenuiflora and down-regulated in P. annua. The smaller number of the genes differentially regulated in P. tenuiflora suggests that the genes regulating bicarbonate tolerance are constitutively expressed in P. tenuiflora.
authors.affiliation: Asian Natural Environmental Science Center (ANESC), the University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan. kobayashi@anesc.u-tokyo.ac.jp.
authors.forename: Shio
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Agricultural and Life Sciences, the University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. asatone@mail.ecc.u-tokyo.ac.jp.
authors.forename: Hina
authors.initials: H
authors.lastname: Satone
authors.affiliation: Graduate School of Agricultural and Life Sciences, the University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. tanengkong@gmail.com.
authors.forename: Engkong
authors.initials: E
authors.lastname: Tan
authors.affiliation: Asian Natural Environmental Science Center (ANESC), the University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan. kurokochi@anesc.u-tokyo.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kurokochi
authors.affiliation: Graduate School of Agricultural and Life Sciences, the University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. asakawa@mail.ecc.u-tokyo.ac.jp.
authors.forename: Shuichi
authors.initials: S
authors.lastname: Asakawa
authors.affiliation: Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China. shenkuiliu@nefu.edu.cn.
authors.forename: Shenkui
authors.initials: S
authors.lastname: Liu
authors.affiliation: Asian Natural Environmental Science Center (ANESC), the University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan. takano@anesc.u-tokyo.ac.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Takano
mesh_terms: D001639:Bicarbonates / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D031785:Poa / Q000235:genetics / Q000254:growth & development / Q000502:physiology; D006109:Poaceae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D054712:Salinity; D013312:Stress, Physiological; D015533:Transcriptional Activation
chemical_list: D001639:Bicarbonates; D010940:Plant Proteins