title: Quantification of protein-materials interaction by soft colloidal probe spectroscopy.
abstract: We present a robust and fast method to quantify the adhesion energy of surface anchored proteins on material surfaces using soft colloidal particles as sensors. The results obtained from studying the adhesion of fibronectin on surfaces with different hydrophobicity were in good agreement with theoretical considerations demonstrating the feasibility of the method.
authors.affiliation: Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany. stephan.schmidt@uni-leipzig.de.
authors.forename: Steve
authors.initials: S
authors.lastname: Martin
authors.forename: Hanqing
authors.initials: H
authors.lastname: Wang
authors.forename: Laura
authors.initials: L
authors.lastname: Hartmann
authors.forename: Tilo
authors.initials: T
authors.lastname: Pompe
authors.forename: Stephan
authors.initials: S
authors.lastname: Schmidt
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D003102:Colloids / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D008299:Maleic Anhydrides / Q000737:chemistry; D008857:Microscopy, Interference; D013499:Surface Properties
chemical_list: D000180:Acrylic Resins; D003102:Colloids; D005353:Fibronectins; D008299:Maleic Anhydrides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C006903:carbopol 940