title: Digital gene expression profiling (DGE) of cadmium-treated Drosophila melanogaster.
abstract: Cadmium is highly toxic and can cause oxidative damage, metabolic disorders, and reduced lifespan and fertility in animals. In this study, we investigated the effects of cadmium in Drosophila melanogaster, performing transcriptome analysis by using tag-based digital gene expression (DGE) profiling. Among 1970 candidate genes, 1443 were up-regulated and 527 were down-regulated following cadmium exposure. Using Gene Ontology analysis, we found that cadmium stress affects three processes: transferase activity, stress response, and the cell cycle. Furthermore, we identified five differentially expressed genes (confirmed by real-time PCR) involved in all three processes: Ald, Cdc2, skpA, tefu, and Pvr. Pathway analysis revealed that these genes were involved in the cell cycle pathway and fat digestion and absorption pathway. This study reveals the gene expression response to cadmium stress in Drosophila, it provides insights into the mechanisms of this response, and it could contribute to our understanding of cadmium toxicity in humans.
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Delong
authors.initials: D
authors.lastname: Guan
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Mo
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China. Electronic address: zhangmin451@snnu.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000818:Animals; D016203:CDC2 Protein Kinase / Q000235:genetics; D002104:Cadmium / Q000633:toxicity*; D002453:Cell Cycle / Q000187:drug effects; D018797:Cell Cycle Proteins / Q000235:genetics; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D063990:Gene Ontology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D044843:SKP Cullin F-Box Protein Ligases / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C434749:SkpA protein, Drosophila; D002104:Cadmium; D044843:SKP Cullin F-Box Protein Ligases; C438368:Pvr protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases; C501876:Mps1 protein, Drosophila; D017346:Protein Serine-Threonine Kinases; C514947:Tefu protein, Drosophila; D016203:CDC2 Protein Kinase
keywords: Cadmium toxicity; Digital gene profiling; Drosophila melanogaster