title: Xylan hydrolysis in Populus trichocarpa × P. deltoides and model substrates during hydrothermal pretreatment.
abstract: Previous studies defined easy and difficult to hydrolyze fractions of hemicellulose that may result from bonds among cellulose, hemicellulose, and lignin. To understand how such bonds affect hydrolysis, Populus trichocarpa × Populus deltoides, holocellulose isolated from P. trichocarpa × P. deltoides and birchwood xylan were subjected to hydrothermal flow-through pretreatment. Samples were characterized by glycome profiling, HPLC, and UPLC-MS. Glycome profiling revealed steady fragmentation and removal of glycans from solids during hydrolysis. The extent of polysaccharide fragmentation, hydrolysis rate, and total xylose yield were lowest for P. trichocarpa × P. deltoides and greatest for birchwood xylan. Comparison of results from P. trichocarpa × P. deltoides and holocellulose suggested that lignin-carbohydrate complexes reduce hydrolysis rates and limit release of large xylooligomers. Smaller differences between results with holocellulose and birchwood xylan suggest xylan-cellulose hydrogen bonds limited hydrolysis, but to a lesser extent. These findings imply cell wall structure strongly influences hydrolysis.
authors.affiliation: Department of Chemical and Environmental Engineering and Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; Department of Chemical and Biological Engineering, 2360 East Mall, Vancouver, British Columbia V6T 1Z3, Canada(1); BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: heather.trajano@ubc.ca.
authors.forename: Heather L
authors.initials: HL
authors.lastname: Trajano
authors.affiliation: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd., Athens, GA 30602, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: siva@ccrc.uga.edu.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Pattathil
authors.affiliation: Chemical Sciences Division, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: tomkinsba@ornl.gov.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Tomkins
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: tschaplinstj@ornl.gov.
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Tschaplinski
authors.affiliation: Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd., Athens, GA 30602, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: hahn@ccrc.uga.edu.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Hahn
authors.affiliation: Chemical Sciences Division, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: vanberkelgj@ornl.gov.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Van Berkel
authors.affiliation: Department of Chemical and Environmental Engineering and Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center, Oak Ridge National Laboratory, PO Box 2008 MS6341, Oak Ridge, TN 37831, USA. Electronic address: cewyman@engr.ucr.edu.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Wyman
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002482:Cellulose / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D003433:Crosses, Genetic; D005947:Glucose / Q000378:metabolism; D054794:Glycomics; D006868:Hydrolysis / Q000187:drug effects; D008031:Lignin / Q000302:isolation & purification; D013058:Mass Spectrometry; D058105:Polymerization / Q000187:drug effects; D032107:Populus / Q000378:metabolism*; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000494:pharmacology*; D014990:Xylans / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism
chemical_list: D012997:Solvents; D014990:Xylans; D014867:Water; D002482:Cellulose; D008031:Lignin; D014994:Xylose; D005947:Glucose
keywords: Hemicellulose; Hydrolysis; Lignin–carbohydrate complex