title: Wheat bran feruloyl oligosaccharides modulate the phase II detoxifying/antioxidant enzymes via Nrf2 signaling.
abstract: The antioxidant activities of wheat bran feruloyl oligosaccharides (FOs) were determined in rats by determining the activities and mRNA expression levels of phase II detoxifying/antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and heme oxygenase-1 (HO-1) in rat organs. FOs was given by gavage at doses of 0.25, 0.5, and 0.75 mmol/kg body weight every day for 15 days. Compared with the control group, the activities of SOD, CAT, and GSH-Px in FOs treatment groups significantly (P<0.05) increased in heart, liver, and kidney. All the FOs treatment also significantly (P<0.05) increased the glutathione (GSH) contents in heart (28-58%), liver (32-71%), and kidney (31-73%) compared with the control. FOs up regulated the mRNA expression levels of SOD, CAT, and HO-1 in organs. Moreover, the immunoblot analysis revealed increased nuclear factor-E2-related factor (Nrf2) protein expression levels in organs and there were positive correlations between the mRNA expression of phase II detoxifying/antioxidant enzymes and the expressions of Nrf2 protein, which demonstrated FOs treatment could modulate the detoxifying/antioxidant enzymes via Nrf2 signaling.
authors.affiliation: Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology & Business University (BTBU), Beijing 100048, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology & Business University (BTBU), Beijing 100048, China. Electronic address: wangjing@th.btbu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology & Business University (BTBU), Beijing 100048, China.
authors.forename: Yingli
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology & Business University (BTBU), Beijing 100048, China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004043:Dietary Fiber; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D008297:Male; D050217:Metabolic Detoxication, Phase II; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D009844:Oligosaccharides / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004043:Dietary Fiber; D051267:NF-E2-Related Factor 2; D009844:Oligosaccharides; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Feruloyl oligosaccharides (FOs); Nrf2; Phase II detoxifying/antioxidant enzymes