title: Dye functionalized cryogel columns for reversible lysozyme adsorption.
abstract: In this study, poly (methyl methacrylate-glycidyl methacrylate) [poly(MMA-GMA)] cryogels were prepared by radical cryocopolymerization of MMA with GMA as a functional comonomer. Reactive Green 19 dye was then attached to the cryogel by nucleophilic substitution reaction, and this dye-attached cryogel column was used for lysozyme adsorption. Characterization of the cryogel was performed by Fourier transform infrared spectroscopy, environmental scanning electron microscopy, Brunauer-Emmett-Teller, and energy dispersive X-ray analysis. Pore size of the cryogels was 15-30 μm and pores were interconnected structure. Attached amount of Reactive Green 19 to cryogel support was calculated as 106.25 μmol/g cryogel. Lysozyme adsorption studies were carried out by using a continuous system. It was found that the maximum amount of lysozyme adsorption (32 mg/g cryogel) obtained from experimental results was found to be approximately same with the calculated Langmuir adsorption capacity (33 mg/g cryogel). Desorption of adsorbed lysozyme was carried out by using 1.5 M NaCl in pH 4.5 acetate buffer, and desorption yield was found to be 97.4%. Cryogels were very stable, and it was found that there was no remarkable reduction in the adsorption capacity at the end of ten adsorption-desorption cycles. As a result, Reactive Green 19-attached cryogels have great advantages such as easy preparation, rapid adsorption, and desorption, being economic and allowing the direct separation of proteins.
authors.affiliation: a Koçarlı Vocational and Training School , Adnan Menderes University , Aydın , Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Uygun
authors.forename: Begüm
authors.initials: B
authors.lastname: Akduman
authors.forename: Deniz Aktaş
authors.initials: DA
authors.lastname: Uygun
authors.forename: Sinan
authors.initials: S
authors.lastname: Akgöl
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D002850:Chromatography, Gel / Q000295:instrumentation*; D004396:Coloring Agents / Q000737:chemistry*; D059985:Cryogels / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000302:isolation & purification / Q000493:pharmacokinetics*; D019904:Polymethyl Methacrylate / Q000737:chemistry; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004396:Coloring Agents; D059985:Cryogels; D004852:Epoxy Compounds; D008689:Methacrylates; D019904:Polymethyl Methacrylate; D009113:Muramidase; C007870:glycidyl methacrylate
keywords: Reactive Green 19; cryogel; dye affinity; lysozyme