title: Hyperspectral imaging for mapping of total nitrogen spatial distribution in pepper plant.
abstract: Visible/near-infrared (Vis/NIR) hyperspectral imaging was employed to determine the spatial distribution of total nitrogen in pepper plant. Hyperspectral images of samples (leaves, stems, and roots of pepper plants) were acquired and their total nitrogen contents (TNCs) were measured using Dumas combustion method. Mean spectra of all samples were extracted from regions of interest (ROIs) in hyperspectral images. Random frog (RF) algorithm was implemented to select important wavelengths which carried effective information for predicting the TNCs in leaf, stem, root, and whole-plant (leaf-stem-root), respectively. Based on full spectra and the selected important wavelengths, the quantitative relationships between spectral data and the corresponding TNCs in organs (leaf, stem, and root) and whole-plant (leaf-stem-root) were separately developed using partial least-squares regression (PLSR). As a result, the PLSR model built by the important wavelengths for predicting TNCs in whole-plant (leaf-stem-root) offered a promising result of correlation coefficient (R) for prediction (RP = 0.876) and root mean square error (RMSE) for prediction (RMSEP = 0.426%). Finally, the TNC of each pixel within ROI of the sample was estimated to generate the spatial distribution map of TNC in pepper plant. The achievements of the research indicated that hyperspectral imaging is promising and presents a powerful potential to determine nitrogen contents spatial distribution in pepper plant.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Ke-Qiang
authors.initials: KQ
authors.lastname: Yu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.
authors.forename: Yan-Ru
authors.initials: YR
authors.lastname: Zhao
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China; Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Beijing, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China; Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Beijing, China.
authors.forename: Yong-Ni
authors.initials: YN
authors.lastname: Shao
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China; Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Beijing, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China; Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: He
mesh_terms: D000465:Algorithms; D002212:Capsicum / Q000033:anatomy & histology / Q000737:chemistry*; D016018:Least-Squares Analysis; D008962:Models, Theoretical; D009584:Nitrogen / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012984:Software; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D009584:Nitrogen