title: [Effects of Biejiajian Pills on Wnt signal pathway signal molecules β-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells].
abstract: OBJECTIVE
  To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma.
  
  
  METHODS
  HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR.
  
  
  RESULTS
  Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities of β-catenin/TCF4 complex.
  
  
  CONCLUSIONS
  Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.
authors.affiliation: Department of Traditional Chinese Medicine, 458 Hospital of PLA, Guangzhou 510602, China.E-mail: hjzwb@aliyun.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Wen
authors.forename: Haitao
authors.initials: H
authors.lastname: Sun
authors.forename: Songqi
authors.initials: S
authors.lastname: He
authors.forename: Yang
authors.initials: Y
authors.lastname: Cheng
authors.forename: Wenyan
authors.initials: W
authors.lastname: Jia
authors.forename: Eryan
authors.initials: E
authors.lastname: Fan
authors.forename: Jie
authors.initials: J
authors.lastname: Pang
mesh_terms: D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D019938:Cyclin D1 / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D051381:Rats; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000378:metabolism*; D051153:Wnt Proteins; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C530104:CCND1 protein, human; C495270:CTNNB1 protein, human; D004365:Drugs, Chinese Herbal; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051153:Wnt Proteins; D051176:beta Catenin; C530159:bie jia jian; D019938:Cyclin D1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2