title: Quantitative proteomics of bronchoalveolar lavage fluid in lung adenocarcinoma.
abstract: The most commonly reported primary lung cancer subtype is adenocarcinoma, which is associated with a poor prognosis and short survival. Proteomic studies on human body fluids such as bronchoalveolar lavage fluid (BALF) have become essential methods for biomarker discovery, examination of tumor pathways and investigation of potential treatments.
  
  
  
  This study used quantitative proteomics to investigate the up-regulation of novel proteins in BALF from patients with primary lung adenocarcinoma in order to identify potential biomarkers.
  
  
  
  BALF samples from individuals with and without primary lung adenocarcinoma were analyzed using liquid chromatography-mass spectrometry.
  
  
  
  One thousand and one hundred proteins were identified, 33 of which were found to be consistently overexpressed in all lung adenocarcinoma samples compared to non-cancer controls. A number of overexpressed proteins have been previously shown to be related to lung cancer progression including S100-A8, annexin A1, annexin A2, thymidine phosphorylase and transglutaminase 2.
  
  
  
  The overexpression of a number of specific proteins in BALF from patients with primary lung adenocarcinoma may be used as a potential biomarker for lung adenocarcinoma.
authors.affiliation: Cancer and Tissue Repair Laboratory, School of Medical Sciences, RMIT University, Bundoora, VIC, Australia Applied Medical Sciences College, Qassim University, Buraidah, Saudi Arabia. E-mail: smtrody@qu.edu.sa
authors.forename: Saleh A
authors.initials: SA
authors.lastname: Almatroodi
authors.affiliation: Institute for Breathing and Sleep, Austin Health, Heidelberg, VIC, Australia.
authors.forename: Christine F
authors.initials: CF
authors.lastname: McDonald
authors.affiliation: Institute for Breathing and Sleep, Austin Health, Heidelberg, VIC, Australia.
authors.forename: Allison L
authors.initials: AL
authors.lastname: Collins
authors.affiliation: Cancer and Tissue Repair Laboratory, School of Medical Sciences, RMIT University, Bundoora, VIC, Australia.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Darby
authors.affiliation: Cancer and Tissue Repair Laboratory, School of Medical Sciences, RMIT University, Bundoora, VIC, Australia.
authors.forename: Dodie S
authors.initials: DS
authors.lastname: Pouniotis
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*; D011379:Prognosis; D011446:Prospective Studies; D040901:Proteomics / Q000379:methods*
keywords: BALF; Lung adenocarcinoma; biomarker; mass spectrophotometry; protein; quantitative proteomics