title: Monitoring the cytosolic entry of cell-penetrating peptides using a pH-sensitive fluorophore.
abstract: We report a simple, effective method to assess the cytosolic delivery efficiency and kinetics of cell-penetrating peptides using a pH-sensitive fluorescent probe, naphthofluorescein.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, OH 43210, USA. pei.3@osu.edu.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Qian
authors.forename: Patrick G
authors.initials: PG
authors.lastname: Dougherty
authors.forename: Dehua
authors.initials: D
authors.lastname: Pei
mesh_terms: D057846:Cell-Penetrating Peptides / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D005434:Flow Cytometry; D019793:Fluorescein / Q000737:chemistry*; D005452:Fluoresceins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D021381:Protein Transport; D013050:Spectrometry, Fluorescence
chemical_list: D057846:Cell-Penetrating Peptides; D005452:Fluoresceins; D005456:Fluorescent Dyes; C000601316:naphthofluorescein; D019793:Fluorescein