title: Maternal fructose consumption alters messenger RNA expression of hippocampal StAR, PBR, P450(11β), 11β-HSD, and 17β-HSD in rat offspring.
abstract: Hippocampal functions such as neuronal protection and synapse formation are positively modulated by neurosteroids, which are synthesized de novo within the brain. However, the mechanisms regulating neurosteroidogenesis remain unclear. Fructose, which is used as a sweetener, affects steroid hormone synthesis in peripheral endocrine organs. This monosaccharide can penetrate the blood-brain barrier and impair hippocampal function. Also, fructose is secreted into milk and is thus delivered to the fetus. Based on these observations, we hypothesized that the hippocampal neurosteroidogenesis in the offspring may be affected by maternal fructose consumption. Female rats were fed with normal water or 20% fructose solution during gestation and lactation. Maternal calorie intake did not change significantly, and no significant change in body weight was observed. The levels of messenger RNAs (mRNAs) for steroidogenic enzymes and proteins in the hippocampus of the offspring were analyzed by real-time reverse transcriptase polymerase chain reaction. Maternal fructose consumption during gestation and lactation increased mRNA levels of P450(11β)-2, 11β-HSD-2, and 17β-HSD-1 in the offspring hippocampus, and reduced levels of mRNAs for StAR, PBR, and 17β-HSD-3. Maternal fructose consumption might influence hippocampal neurosteroidogenesis in offspring.
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ohashi
authors.affiliation: Department of Joint Research Laboratory of Clinical Medicine, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Ando
authors.affiliation: Department of Biochemistry, Fujita Health University School of Medicine, Toyoake, Japan. Electronic address: mntneiji@fujita-hu.ac.jp.
authors.forename: Eiji
authors.initials: E
authors.lastname: Munetsuna
authors.affiliation: Department of Hygiene, Fujita Health University School of Medicine, Toyoake, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Yamada
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Mirai
authors.initials: M
authors.lastname: Yamazaki
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Ayuri
authors.initials: A
authors.lastname: Nagura
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Taromaru
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Department of Public Health, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Department of Clinical Biochemistry, Fujita Health University School of Health Sciences, Toyoake, Japan.
authors.forename: Ryouji
authors.initials: R
authors.lastname: Teradaira
mesh_terms: D000818:Animals; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D005260:Female; D047109:Fetal Development / Q000187:drug effects* / Q000235:genetics; D005632:Fructose / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D006728:Hormones / Q000235:genetics / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism; D011247:Pregnancy; D039401:Prenatal Nutritional Physiological Phenomena; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D013256:Steroids / Q000378:metabolism*
chemical_list: D004040:Dietary Carbohydrates; D006728:Hormones; D018377:Neurotransmitter Agents; D012333:RNA, Messenger; D013256:Steroids; D005632:Fructose
keywords: Hippocampus; Maternal fructose consumption; Neurosteroid; PBR; Rat offspring; StAR