title: Anti-septic activity of α-cubebenoate isolated from Schisandra chinensis.
abstract: Sepsis is a life-threatening, infectious, systemic inflammatory disease. In this study, we investigated the therapeutic effect of α-cubebenoate, a novel compound isolated from Schisandra chinensis against polymicrobial sepsis in a cecal ligation and puncture (CLP) experimental model. Administration of α-cubebenoate strongly enhanced survival in the CLP model. α-cubebenoate administration also markedly blocked CLP-induced lung inflammation and increased bactericidal activity by enhancing phagocytic activity and hydrogen peroxide generation in mouse bone marrow-derived macrophages and neutrophils. Expression of two important inflammatory cytokines, IL-1β and IL-6, was strongly increased in the CLP model, and this was dramatically blocked by α-cubebenoate. Lymphocyte apoptosis and caspase-3 activation, which are associated with immune paralysis during sepsis, were markedly attenuated by α-cubebenoate. Taken together, our findings indicate that α-cubebenoate, a natural compound isolated from Schisandra chinensis, is a powerful potential anti-septic agent.
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Minsoo
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Sung Kyun
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746, Korea.
authors.forename: Sang Doo
authors.initials: SD
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746; Mitochondria Hub Regulation Center, Dong-A University, Busan 602-714, Korea.
authors.forename: Ha Young
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju 565-851, Korea.
authors.forename: Jae Sam
authors.initials: JS
authors.lastname: Hwang
authors.affiliation: Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University, Miryang 627-706, Korea.
authors.forename: Young Whan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, Sungkyunkwan University, Suwon 440-746; Mitochondria Hub Regulation Center, Dong-A University, Busan 602-714; Samsung Advanced Institute for Health Sciences and Technology, Sungkyunkwan University, Seoul 135-710, Korea.
authors.forename: Yoe-Sik
authors.initials: YS
authors.lastname: Bae
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001770:Blood Bactericidal Activity / Q000187:drug effects; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D032141:Schisandra / Q000737:chemistry; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045789:Sesquiterpenes, Guaiane / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000891:Anti-Infective Agents, Local; D016207:Cytokines; D017382:Reactive Oxygen Species; D045789:Sesquiterpenes, Guaiane; C000598129:alpha-cubebenoate