title: [Effect of Escherichia coli secreted metabolites on functional activity of human neutrophils against the background of estriol effect].
abstract: OBJECTIVE
  Study the effect of Escherichia coli acellular metabolites of various phases of development on phagocytic activity of neutrophils against the background of pregnancy hormone effect--estriol (E3).
  
  
  METHODS
  E. coli K12 (wt) metabolites were selected at the end of adaptation and logarithmic growth phases by filtration after cultivation at 37 degrees C in LB broth. Neutrophils of heparinized venous blood of healthy non-pregnant women (follicular phase, n = 8) were incubated for 1 hour with E3 at 2 ng/ml and 20 ng/ml, then 30 minutes with E. coli metabolites, LB medium or Hanks' solution at 37 degrees C. Phagocytic activity evaluation was carried out by inhibition of bioluminescence of E. coli K12 TG1 lux+.
  
  
  RESULTS
  Exometabolites of logarithmic growth phase of E. coli culture inhibited neutrophil phagocytosis after 40 - 60 minutes of incubation in contrast to metabolites of adaptation phase compared with LB medium. Neutrophil cultivation after hormone treatment in LB medium that has the ability to stimulate neutrophil phagocytosis compared with Hanks' solution did not alter their phagocytic activity. However inhibiting effect of E3 at 20 ng/ml on neutrophil phagocytosis compared with control was exhibited in Hanks' solution (at 50 - 60 minutes) and after the effect of E. coli adaptation phase metabolites (at 40 - 60 minutes).
  
  
  CONCLUSIONS
  E3 at the level extrapolated from its level at III trimester of pregnancy could facilitate the reduction of antimicrobial potential of neutrophils at the early stages of growth of pathogenic microorganisms.
authors.forename: S V
authors.initials: SV
authors.lastname: Shirshev
authors.forename: I L
authors.initials: IL
authors.lastname: Maslennikova
authors.forename: I V
authors.initials: IV
authors.lastname: Nekrasova
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism* / Q000472:pathogenicity; D004964:Estriol / Q000096:biosynthesis* / Q000378:metabolism; D005260:Female; D006801:Humans; D064307:Microbiota; D009504:Neutrophils / Q000378:metabolism* / Q000382:microbiology; D010587:Phagocytosis; D011247:Pregnancy
chemical_list: D000890:Anti-Infective Agents; D004964:Estriol