title: Orange juice intake during a fatty meal consumption reduces the postprandial low-grade inflammatory response in healthy subjects.
abstract: OBJECTIVE
  Evidence associates polyphenol-rich foods to reduction of low-grade inflammation and mortality for cardiovascular disease, the mechanisms underlying such effects being still unclear. Consumption of a fatty meal by healthy volunteers induces rapid and reversible low-grade inflammation. The aim of the present study was to evaluate the effect of orange juice on cellular modifications induced by a fatty meal.
  
  
  RESULTS
  18 apparently healthy subjects consumed a fatty meal, during which they drunk orange juice, either blond or red, or water, according to a randomized cross-over design. Two hours after the end of the fatty meal, both white blood cell (WBC) and platelet counts significantly increased (12.5 and 5%, respectively), while mean platelet volume decreased and a 25% release of myeloperoxidase (MPO) from polymorphonuclear leukocyte occurred. Both juices significantly prevented WBC increase and MPO degranulation, in respect to control. Triglycerides significantly increased (42%) after the fatty meal, but at a lower extent when red orange juice was consumed with the meal (20%), in respect to blond orange juice or control. This effect was statistically significant in the subgroup of 8 subjects with hypertriglyceridemia. Vascular stiffness (augmentation index), measured by Endo-PAT2000, significantly decreased after the meal only in conjunction with red orange juice.
  
  
  CONCLUSIONS
  In healthy subjects the concomitant intake of orange juice may prevent the low-grade inflammatory reaction induced by a fatty meal, at cellular and possibly at vascular function levels. The relative role of different polyphenols on the observed effects of orange juices remains to be established.
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy. Electronic address: chiara.cerletti@neuromed.it.
authors.forename: C
authors.initials: C
authors.lastname: Cerletti
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy; Research Centre in Epidemiology and Preventive Medicine EPIMED, Department of Clinical and Experimental Medicine, University of Insubria, Varese, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gianfagna
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tamburrelli
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Curtis
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: M
authors.initials: M
authors.lastname: D'Imperio
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: W
authors.initials: W
authors.lastname: Coletta
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Giordano
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Lorenzet
authors.affiliation: Consiglio per la Ricerca e Sperimentazione in Agricoltura-Centro di Ricerca per l'Agrumicoltura e le Colture Mediterranee (CT), Italy.
authors.forename: P
authors.initials: P
authors.lastname: Rapisarda
authors.affiliation: Consiglio per la Ricerca e Sperimentazione in Agricoltura-Centro di Ricerca per l'Agrumicoltura e le Colture Mediterranee (CT), Italy.
authors.forename: G
authors.initials: G
authors.lastname: Reforgiato Recupero
authors.affiliation: Research Laboratories, Fondazione di Ricerca e Cura "Giovanni Paolo II", Catholic University, Campobasso, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Rotilio
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy.
authors.forename: L
authors.initials: L
authors.lastname: Iacoviello
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy.
authors.forename: G
authors.initials: G
authors.lastname: de Gaetano
authors.affiliation: Department of Epidemiology and Prevention, IRCCS Istituto Neurologico Mediterraneo NEUROMED, Pozzilli (IS), Italy.
authors.forename: M B
authors.initials: MB
authors.lastname: Donati
mesh_terms: D000328:Adult; D001628:Beverages; D002318:Cardiovascular Diseases / Q000517:prevention & control*; D032084:Citrus sinensis / Q000378:metabolism*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000178:diet therapy*; D008297:Male; D062407:Meals; D009195:Peroxidase; D019518:Postprandial Period; D012307:Risk Factors; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D009195:Peroxidase
keywords: Fatty meal; Low-grade inflammation; Myeloperoxidase; Orange juice; Vascular function