title: Production of monoclonal antibody against EP0 protein of pseudorabies virus and determination of its recognized epitope.
abstract: Early protein 0 (EP0) is especially important for modulating PRV gene expression and reactivation from the latent state, but the mechanisms have not been elucidated. In this study, six monoclonal antibodies (MAbs) against EP0 protein of PRV were generated and their characterizations were investigated. Western blot analysis showed all six MAbs could react with immunizing antigen, but only 2B12 and 2C6 could react with native EP0 protein from PRV-infected cells. ELISA additivity tests revealed that at least three epitopes in EP0 were defined by six MAbs. The epitope recognized by MAb 2B12 was further identified in 287-292 aa of EP0 protein using a series of expressed overlapping peptides. These MAbs may provide valuable tools for further research of the functions of EP0 in PRV infection.
authors.affiliation: 1 College of Veterinary Medicine, South China Agricultural University , Guangzhou, P.R. China .
authors.forename: Keyu
authors.initials: K
authors.lastname: Xiang
authors.forename: Yi
authors.initials: Y
authors.lastname: Cheng
authors.forename: Meizhen
authors.initials: M
authors.lastname: Zhou
authors.forename: Leilei
authors.initials: L
authors.lastname: Sun
authors.forename: Yikuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Baoshi
authors.initials: B
authors.lastname: Zhang
authors.forename: Yongwen
authors.initials: Y
authors.lastname: Luo
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ju
mesh_terms: D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D014764:Viral Proteins; C070914:early protein O, Pseudorabies virus