title: Antibody profiling of bipolar disorder using Escherichia coli proteome microarrays.
abstract: To profile plasma antibodies of patients with bipolar disorder (BD), an E. coli proteome microarray comprising ca. 4200 proteins was used to analyze antibody differences between BD patients and mentally healthy controls (HCs). The plasmas of HCs and patients aged 18-45 years with bipolar I disorder (DSM-IV) in acute mania (BD-A) along with remission (BD-R) were collected. The initial samples consisting of 19 BD-A, 20 BD-R, and 20 HCs were probed with the microarrays. After selecting protein hits that recognized the antibody differences between BD and HC, the proteins were purified to construct BD focus arrays for training diagnosis committees and validation. Additional six BD-A, six BD-R, six HCs, and nine schizophrenic disorder (SZ, as another psychiatric control) samples were individually probed with the BD focus arrays. The trained diagnosis committee in BD-A versus HC combined top six proteins, including rpoA, thrA, flhB, yfcI, ycdU, and ydjL. However, the optimized committees in BD-R versus HC and BD-A versus BD-R were of low accuracy (< 0.6). In the single blind test using another four BD-A, four HC, and four SZ samples, the committee of BD-A versus HC was able to classify BD-A versus HC and SZ with 75% sensitivity and 80% specificity that both HC and SZ were regarded as negative controls. The consensus motif of the six proteins, which form the committee of BD-A versus HC, is [KE]DIL[AG]L[LV]I[NL][IC][SVKH]G[LV][VN][LV] by Gapped Local Alignment of Motifs. We demonstrated that the E. coli proteome microarray is capable of screening BD plasma antibody differences and the selected proteins committee was successfully used for BD diagnosis with 79% accuracy.
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Po-Chung
authors.initials: PC
authors.lastname: Chen
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Guan-Da
authors.initials: GD
authors.lastname: Syu
authors.affiliation: §Department of Psychiatry, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; ¶Department of Psychiatry and Psychiatric Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Kuo-Hsuan
authors.initials: KH
authors.lastname: Chung
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Ho
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Feng-Hsiang
authors.initials: FH
authors.lastname: Chung
authors.affiliation: §Department of Psychiatry, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; ¶Department of Psychiatry and Psychiatric Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Pao-Huan
authors.initials: PH
authors.lastname: Chen
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Jyun-Mu
authors.initials: JM
authors.lastname: Lin
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan;
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Chen
authors.affiliation: §Department of Psychiatry, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; ¶Department of Psychiatry and Psychiatric Research Center, Taipei Medical University Hospital, Taipei, Taiwan.
authors.forename: Shang-Ying
authors.initials: SY
authors.lastname: Tsai
authors.affiliation: From the ‡Graduate Institute of Systems Biology and Bioinformatics, National Central University, Taiwan; cchen103@gmail.com.
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000378:metabolism*; D001714:Bipolar Disorder / Q000097:blood / Q000175:diagnosis* / Q000473:pathology; D016384:Consensus Sequence; D004926:Escherichia coli / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D008875:Middle Aged; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods*; D012559:Schizophrenia / Q000097:blood / Q000175:diagnosis*; D016037:Single-Blind Method; D055815:Young Adult
chemical_list: D000906:Antibodies