title: The Dishevelled, EGL-10 and pleckstrin (DEP) domain-containing protein DEPDC7 binds to CARMA2 and CARMA3 proteins, and regulates NF-κB activation.
abstract: The molecular complexes containing BCL10, MALT1 and CARMA proteins (CBM complex) have been recently identified as a key component in the signal transduction pathways that regulate activation of Nuclear Factor kappaB (NF-κB) transcription factor. Herein we identified the DEP domain-containing protein DEPDC7 as cellular binding partners of CARMA2 and CARMA3 proteins. DEPDC7 displays a cytosolic distribution and its expression induces NF-κB activation. Conversely, shRNA-mediated abrogation of DEPDC7 results in impaired NF-κB activation following G protein-coupled receptors stimulation, or stimuli that require CARMA2 and CARMA3, but not CARMA1. Thus, this study identifies DEPDC7 as a CARMA interacting molecule, and provides evidence that DEPDC7 may be required to specifically convey on the CBM complex signals coming from activated G protein-coupled receptors.
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy.
authors.forename: Egildo Luca
authors.initials: EL
authors.lastname: D'Andrea
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Ferravante
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Ivan
authors.initials: I
authors.lastname: Scudiero
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Zotti
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Reale
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Pizzulo
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy; Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Luigi Regenburgh
authors.initials: LR
authors.lastname: De La Motte
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: De Maio
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Pellegrino
authors.initials: P
authors.lastname: Mazzone
authors.affiliation: Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Telesio
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy; Biogem Consortium, Via Camporeale, 83031 Ariano Irpino (AV), Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Vito
authors.affiliation: Dipartimento di Scienze e Tecnologie, Università degli Studi del Sannio, Via Port'Arsa 11, 82100 Benevento, Italy; SannioTech Consortium, Strada Statale Appia, Benevento, Italy.
authors.forename: Romania
authors.initials: R
authors.lastname: Stilo
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry; D001798:Blood Proteins / Q000737:chemistry; D053468:CARD Signaling Adaptor Proteins / Q000276:immunology* / Q000378:metabolism; D000072261:Dishevelled Proteins; D005786:Gene Expression Regulation; D006162:Guanylate Cyclase / Q000276:immunology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D016328:NF-kappa B / Q000276:immunology*; D010750:Phosphoproteins / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D001798:Blood Proteins; D053468:CARD Signaling Adaptor Proteins; C430880:CARD10 protein, human; C000603056:DEPDC7 protein, human; D000072261:Dishevelled Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D016328:NF-kappa B; D010750:Phosphoproteins; C039077:platelet protein P47; C426243:CARD14 protein, human; D006162:Guanylate Cyclase