title: Identification of mud crab reovirus VP12 and its interaction with the voltage-dependent anion-selective channel protein of mud crab Scylla paramamosain.
abstract: Mud crab reovirus (MCRV) is the causative agent of a severe disease in cultured mud crab (Scylla paramamosain), which has caused huge economic losses in China. MCRV is a double-stranded RNA virus with 12 genomic segments. In this paper, SDS-PAGE, mass spectrometry and Western blot analyses revealed that the VP12 protein encoded by S12 gene is a structural protein of MCRV. Immune electron microscopy assay indicated that MCRV VP12 is a component of MCRV outer shell capsid. Yeast two hybrid cDNA library of mud crab was constructed and mud crab voltage-dependent anion-selective channel (mcVDAC) was obtained by MCRV VP12 screening. The full length of mcVDAC was 1180 bp with an open reading frame (ORF) of 849 bp encoding a 282 amino acid protein. The mcVDAC had a constitutive expression pattern in different tissues of mud crab. The interaction between MCRV VP12 and mcVDAC was determined by co-immunoprecipitation assay. The results of this study have provided an insight on the mechanisms of MCRV infection and the interactions between the virus and mud crab.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Hai-Dong
authors.initials: HD
authors.lastname: Xu
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Su
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Zou
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Liu
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Wen-Rui
authors.initials: WR
authors.lastname: Yan
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Yuan
authors.affiliation: Fisheries College, Guangdong Ocean University, Zhanjiang, Guangdong, People's Republic of China.
authors.forename: Siuming Francis
authors.initials: SF
authors.lastname: Chan
authors.affiliation: Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA.
authors.forename: Xiao-Qiang
authors.initials: XQ
authors.lastname: Yu
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China; School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: He
authors.affiliation: School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou, Guangdong, People's Republic of China. Electronic address: lsswsp@mail.sysu.edu.cn.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Weng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D003386:Brachyura / Q000378:metabolism / Q000821:virology; D004926:Escherichia coli / Q000235:genetics; D005880:Gills / Q000378:metabolism; D006367:HeLa Cells; D043143:Hepatopancreas / Q000378:metabolism; D006801:Humans; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D012087:Reoviridae / Q000502:physiology / Q000648:ultrastructure; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism*; D050994:Voltage-Dependent Anion Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: D060829:Arthropod Proteins; D015678:Viral Structural Proteins; D050994:Voltage-Dependent Anion Channels
keywords: Interaction; Mud crab reovirus; Mud crab voltage-dependent anion-selective channel; Structure protein VP12