title: Molecular characterization of Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae) from goats in the western part of India by LSU of nuclear ribosomal DNA.
abstract: The rumen parasite, Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae), is a highly pathogenic trematode parasite of goat (Capra hircus). It sucks blood that causes acute disease like anemia, and severe economic losses occur due to morbidity and mortality of the ruminant infected by these worms. The study of these rumen paramphistomes, their infection, and public health importance remains unclear in India especially in the western part of state Uttar Pradesh (U.P.), Meerut, India, where the goat meat consumption is very high. This paper provides the molecular characterization of G. crumenifer recovered from the rumen of Capra hircus from Meerut, U.P., India by the partial sequence of 28S rDNA. Nucleotide sequence similarity searching on BLAST of 28S rDNA from parasites showed the highest identity with those of G. crumenifer from the same host Capra hircus. This is the first report of molecular identification of G. crumenifer from this part of India.
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.) 250004, India.
authors.forename: Ashwani
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.) 250004, India. ; Veterinary Medical Research Institute, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
authors.forename: Anshu
authors.initials: A
authors.lastname: Chaudhary
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.) 250004, India.
authors.forename: Chandni
authors.initials: C
authors.lastname: Verma
authors.affiliation: Molecular Taxonomy Laboratory, Department of Zoology, Chaudhary Charan Singh University, Meerut (U.P.) 250004, India.
authors.forename: Hridaya Shanker
authors.initials: HS
authors.lastname: Singh
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D018509:DNA, Helminth / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D015511:Goat Diseases / Q000469:parasitology*; D006041:Goats; D007194:India; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D010986:Platyhelminths / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D012417:Rumen / Q000469:parasitology; D017422:Sequence Analysis, DNA; D014201:Trematode Infections / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S
keywords: Gastrothylax crumenifer; India; Uttar Pradesh; goat (Capra hircus); ribosomal DNA