title: Chromosome aberrations and spermatogenic disorders in mice with Robertsonian translocation (11; 13).
abstract: OBJECTIVE
  To determine the diagnostic features of Robertsonian (Rob) translocation (11; 13) in mice and the mechanisms underlying the effect on spermatogenesis and reproductive decline.
  
  
  METHODS
  A Rob translocation (11; 13) mouse model was established by cross-breeding, and confirmed by chromosome analysis. Chromosome aberrations and translocation patterns were identified in mice with Rob translocation (11; 13) by fluorescence in situ hybridization (FISH). Spermatogenic disorders were investigated at different stages of spermatogenesis. Immunofluorescent analysis was performed on sections of testis and epididymis specimens during spermatogenic meiosis. The weight of the testes and reproductive decline were recorded.
  
  
  RESULTS
  The crossed Rob translocation (11; 13) mouse has 39 chromosomes, including a fusion chromosome (included chromosomes 11 and 13) using dual color FISH. There was no difference in the distribution pattern of SYCP3 and γH2AX in spermatocytes between Rob translocation and wild-type mice; however, round haploid spermatids presented characteristic morphologic changes of apoptosis and the number of haploid spermatids was decreased. Furthermore, the immature germ cells were released into the epididymis and the number of mature sperm was reduced.
  
  
  CONCLUSIONS
  Chromosome aberrations and spermatogenic disorders may result from apoptosis of round haploid spermatids and a reduced number of mature sperm in Rob translocation (11; 13) mice. Abnormal sperm and reduced number of sperm may be one of the main reasons for reproductive decline and male infertility in Rob translocation (11; 13) mice.
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Xinjie
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Xiaohu
authors.initials: X
authors.lastname: Jin
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Junsheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Peking University Third Hospital Beijing, China ; Key Laboratory of Assisted Reproduction, Ministry of Education Beijing, China ; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction Beijing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002869:Chromosome Aberrations; D033481:Chromosomes, Mammalian; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D051379:Mice; D013091:Spermatogenesis / Q000235:genetics*; D013094:Spermatozoa; D014178:Translocation, Genetic / Q000235:genetics*
keywords: Infertility; Robertsonian translocation; mouse; spermatogenesis