title: Simultaneous determination of ten flavonoids of crude and wine-processed Radix Scutellariae aqueous extracts in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study.
abstract: Radix Scutellariae (RS) is a herbal medicine with various pharmacological activities to treat inflammation, respiratory and gastrointestinal infections, etc. In this study, a rapid, sensitive and selective UPLC-ESI-MS/MS method was developed for simultaneous determination of 10 flavonoids - scutellarin, scutellarein, chrysin, wogonin, baicalein, apigenin, wogonoside, oroxylin A-7-O-glucuronide, oroxylin A and baicalin - from RS aqueous extracts in rat plasma with propyl paraben as internal standard (IS). Chromatographic separation was achieved on a C18 column using gradient elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection was performed in multiple reaction monitoring mode using electrospray ionization in negative mode. The validated method showed good linearity over a wide concentration range (r >0.9935). The intra- and interday assay variabilities were <9.5% and <12.4% for all analytes, respectively. The extraction recovery ranged from 71.2 to 89.7% for each analyte and IS. This method was successfully applied to pharmacokinetic comparision after oral administration of crude and wine-processed RS aqueous extracts. There were significant differences in some pharmacokinetic parameters of most analytes between crude and wine-processed RS. This suggested that wine-processing exerted effects absorption of most flavonoids.
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Xiao-Cui
authors.initials: XC
authors.lastname: Qian
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Yong-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Li
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Yang
authors.affiliation: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China.|Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing, 210023, China.
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014920:Wine / Q000032:analysis*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids
keywords: Radix Scutellariae; UPLC-ESI-MS/MS; flavonoids, wine processing; pharmacokinetics