title: Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library.
abstract: Mammalian cell nuclei contain multiple granular structures, which are termed nuclear bodies. These structures are involved in various molecular events in the nucleus; they provide platforms for biogenesis of macromolecular complexes that are essential for gene expression, such as the ribosome and spliceosome; they act as reservoirs of various regulatory factors; and they are involved in the regulation of specific gene loci. Nuclear bodies are usually visualized by immunostaining for specific marker proteins. Although each type of nuclear body contains a distinct set of proteins, the protein components of most types of nuclear bodies remain to be identified. This chapter introduces a new approach to identify the protein components of specific types of nuclear bodies.
authors.affiliation: Institute for Genetic Medicine, Hokkaido University, Kita-15, Nishi-7, Kita-Ku, Sapporo, 060-0815, Japan, hirose@igm.hokudai.ac.jp.
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Hirose
authors.forename: Naoki
authors.initials: N
authors.lastname: Goshima
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D015723:Gene Library; D015894:Genome, Human; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D045586:Intranuclear Inclusion Bodies / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods; D009687:Nuclear Proteins / Q000378:metabolism*; D010957:Plasmids / Q000737:chemistry / Q000235:genetics
chemical_list: D005456:Fluorescent Dyes; D009687:Nuclear Proteins