title: Wheat proteomics: proteome modulation and abiotic stress acclimation.
abstract: Cellular mechanisms of stress sensing and signaling represent the initial plant responses to adverse conditions. The development of high-throughput "Omics" techniques has initiated a new era of the study of plant molecular strategies for adapting to environmental changes. However, the elucidation of stress adaptation mechanisms in plants requires the accurate isolation and characterization of stress-responsive proteins. Because the functional part of the genome, namely the proteins and their post-translational modifications, are critical for plant stress responses, proteomic studies provide comprehensive information about the fine-tuning of cellular pathways that primarily involved in stress mitigation. This review summarizes the major proteomic findings related to alterations in the wheat proteomic profile in response to abiotic stresses. Moreover, the strengths and weaknesses of different sample preparation techniques, including subcellular protein extraction protocols, are discussed in detail. The continued development of proteomic approaches in combination with rapidly evolving bioinformatics tools and interactive databases will facilitate understanding of the plant mechanisms underlying stress tolerance.
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization Tsukuba, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.affiliation: National Institute of Crop Science, National Agriculture and Food Research Organization Tsukuba, Japan.
authors.forename: Abu H M
authors.initials: AH
authors.lastname: Kamal
authors.affiliation: Plant Stress Biology Lab, Department of Botany, West Bengal State University Kolkata, India.
authors.forename: Zahed
authors.initials: Z
authors.lastname: Hossain
keywords: abiotic stress; proteomics; review; wheat