title: Comparison of two kinds of orthotopic xenograft models for human ovarian cancer.
abstract: OBJECTIVE
  The aim of this study was to compare the characteristics of two orthotopic xenograft models established with human epithelial ovarian cancer solid tumor tissue slices and human ovarian carcinoma cell line OVCAR-3.
  
  
  METHODS
  Tumor tissues and cell line OVCAR3 of human epithelial ovarian cancer were grown in subcutaneous tissue and the subcutaneous tumor source was fetched and inoculated in ovarian capsule of nude mice under microscope to establish the orthotopic implantation model. At four and eight weeks after modeling, the orthotopic tumor formation rate, tumor diameter, metastasis rate outside the ovary, incidence rate of ascites, and CA125 levels in the two models were observed.
  
  
  RESULTS
  The orthotopic tumor formation rate in the solid tumor slices group (60.0%) was significantly lower than that in the cell line group (85.0%, p < 0.05). However, the tumor diameter, metastasis rate outside the ovary, incidence rate of ascites, and CA125 levels in the solid tumor slices group (2.4 +/- 0.61 cm, 75.0%, 50.0%, and 80.13 +/- 11.26 U/ml, respectively) were remarkably higher than those in the cell line group (1.6 +/- 0.53 cm, 52.9%, 29.4%, and 36.5 +/- 6.71 U/ml, respectively) (p < 0.05, respectively).
  
  
  CONCLUSIONS
  There are differences between the two orthotopic xenograft models established with human epithelial ovarian cancer solid tumor tissue slices and human ovarian carcinoma cell line OVCAR-3. The biological characteristics of the solid tumor slices model are more similar to human ovarian cancer.
authors.affiliation: Department of Gynecology,  Hainan Provincial People's Hospital, Haikou, China.  genhaizhu@126.com
authors.forename: Genhai
authors.initials: G
authors.lastname: Zhu
authors.forename: Lan
authors.initials: L
authors.lastname: Hong
authors.forename: Shengtan
authors.initials: S
authors.lastname: Wang
authors.lastname: Junhong Cai
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Yang
authors.forename: Maozhong
authors.initials: M
authors.lastname: Yao
mesh_terms: D000818:Animals; D018394:CA-125 Antigen / Q000097:blood; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D005260:Female; D064593:Heterografts; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D014183:Transplantation, Heterologous
chemical_list: D018394:CA-125 Antigen