title: Loss of MXD3 induces apoptosis of Reh human precursor B acute lymphoblastic leukemia cells.
abstract: MXD3 is a transcription factor that plays an important role in proliferation of human DAOY medulloblastoma cells. Here, we demonstrate that MXD3 is highly enriched in human precursor B acute lymphoblastic leukemia (preB ALL) samples compared to mobilized peripheral blood mononuclear cells, bone marrow, or hematopoietic stem cells from healthy donors. MXD3 knock-down in the preB ALL cell line Reh resulted in decreased cell numbers with no change in G0/G1, S or G2/M populations but increased apoptosis compared to control cells. Our results suggest that MXD3 is important for survival of Reh preB ALL cells, possibly as an anti-apoptotic factor.
authors.affiliation: Department of Pharmacology, UC Davis School of Medicine, Davis, CA, USA.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Barisone
authors.affiliation: Department of Pediatrics, Hematology/Oncology Section, UC Davis School of Medicine, Sacramento, CA, USA; Stem Cell Program and Institute of Regenerative Cures, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Noriko
authors.initials: N
authors.lastname: Satake
authors.affiliation: Department of Pediatrics, Hematology/Oncology Section, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Carly
authors.initials: C
authors.lastname: Lewis
authors.affiliation: Department of Pediatrics, Hematology/Oncology Section, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Connie
authors.initials: C
authors.lastname: Duong
authors.affiliation: Department of Pediatrics, Hematology/Oncology Section, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Cathy
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Biochemistry and Molecular Medicine, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
authors.affiliation: Stem Cell Program and Institute of Regenerative Cures, UC Davis School of Medicine, Sacramento, CA, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Nolta
authors.affiliation: Department of Pharmacology, UC Davis School of Medicine, Davis, CA, USA. Electronic address: ediaz@ucdavis.edu.
authors.forename: Elva
authors.initials: E
authors.lastname: Dίaz
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D016022:Case-Control Studies; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism; D008527:Medulloblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics*
chemical_list: C543450:MXD3 protein, human; D012097:Repressor Proteins
keywords: Apoptosis; MXD3; Precursor B acute lymphoblastic leukemia; Proliferation