title: Glucose oxidase adsorption performance of carbonaceous mesocellular foams prepared with different carbon sources.
abstract: Several carbonaceous mesocellular foams (C-MCFs) were prepared with MCF-silica as template using the carbon precursors of sucrose, furfuryl alcohol and lab-made phenolic resin, and the corresponding C-MCFs were named as C-MCF-Suc, C-MCF-FA and C-MCF-PR, respectively. The results of SEM, transmission electron microscopy, N2 adsorption-desorption and energy-dispersive X-ray measurements indicated that the C-MCFs prepared from different carbon source appeared morphologically with different degree of order and different pore distribution. The C-MCF-FA exhibited the highest ordered structure and the smallest pore distribution among the foams. The optimum conditions for adsorption of C-MCFs on glucose oxidase (GOD) were also studied, and the maximum adsorbance was determined. The adsorption of GOD on C-MCF-FA was performed at different pH with different GOD concentrations. The maximum adsorption (423.3 mg g(-1)) was observed near the isoelectric point of the GOD (pI ≈ 5.0) with a GOD concentration of 6.0 mg mL(-1), suggesting that the GOD adsorption on C-MCFs might be affected strongly by the electric repulsion between the GOD molecules. Moreover, GOD adsorption performances on different C-MCFs revealed that both the pore size and the pore volume played important roles in the adsorption process, and the window size of C-MCFs dominated the residual immobilized amounts of GOD. Compared to the other two C-MCFs, the C-MCF-FA with a smaller window pore (10 nm) and higher volume (1.40 cm(3) g(-1)) exhibited the highest GOD adsorption and catalytic activity. Furthermore, the immobilized GOD exhibited improved thermal and storable stabilities. Thus the C-MCF-FA could be served as the prospective GOD carrier material used in enzymatic fuel cells.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Zhou
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Pu
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Luo
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China. Electronic address: nic0700@scu.edu.cn.
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhuang
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liao
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Zhongbin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Yadong
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000327:Adsorption; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism*; D005557:Formaldehyde / Q000737:chemistry; D005663:Furans / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010636:Phenols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry
chemical_list: D005663:Furans; D010636:Phenols; D011108:Polymers; D005557:Formaldehyde; D013395:Sucrose; D002244:Carbon; D012822:Silicon Dioxide; C011529:phenol-formaldehyde resin; C012986:furfuryl alcohol; D005949:Glucose Oxidase
keywords: Carbonaceous mesocellular foams; Furfuryl alcohol; Glucose oxidase adsorption; Phenolic resin; Solution pH and concentration; Sucrose