title: The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study.
abstract: BACKGROUND
  Severe burn induces systemic inflammation and reactive oxygen species leading to lipid peroxidation which may play role in remote organs injury. Sildenafil is a selective and potent inhibitor of cyclic guanosine monophosphate specific phosphodiesterase-5. Sildenafil reduces oxidative stress and inflammation in distant organs. The aim of the present study was to evaluate the effects of different dosages of sildenafil in remote organs injury.
  
  
  METHODS
  A total of thirty-two rats were randomly divided into four equal groups. The groups were designated as follows: Sham, Control, 10, and T20 mg/kg sildenafil treatment groups. Levels of malondialdehyde (MDA), vascular endothelial growth factor (VEGF), VEGF receptor (Flt-1), activities of glutathione peroxidase (Gpx), levels of total antioxidative capacity (TAC), and total oxidant status (TOS) were measured in both tissues and serum, and a semi-quantitative scoring system was used for the evaluation of histopathological findings.
  
  
  RESULTS
  Sildenafil increased levels of Gpx, and Flt-1, and decreased MDA and VEGF levels in tissues. Sildenafil also increased serum levels of TAC and Flt-1 and decreased TOS, OSI, and VEGF.
  
  
  CONCLUSIONS
  Sildenafil decreased inflammation scores in remote organs in histopathological evaluation. It has protective effects in severe burn-related remote organ injuries by decreasing oxidative stress and inflammation.
authors.affiliation: Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas, Turkey. dralihan20@hotmail.com.
authors.forename: Ali Kağan
authors.initials: AK
authors.lastname: Gökakın
authors.affiliation: Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Atabey
authors.affiliation: Department of Biochemistry, Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
authors.forename: Koksal
authors.initials: K
authors.lastname: Deveci
authors.affiliation: Department of Biochemistry, Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
authors.forename: Enver
authors.initials: E
authors.lastname: Sancakdar
authors.affiliation: Department of Pathology, Cumhuriyet University Faculty of Veterinary, Sivas, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tuzcu
authors.affiliation: Department of Anesthesiology, Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
authors.forename: Cevdet
authors.initials: C
authors.lastname: Duger
authors.affiliation: Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Topcu
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology*; D005260:Female; D007249:Inflammation / Q000517:prevention & control*; D007668:Kidney / Q000293:injuries / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000293:injuries / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000068677:Sildenafil Citrate / Q000008:administration & dosage / Q000627:therapeutic use*; D017695:Soft Tissue Injuries / Q000473:pathology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014665:Vasodilator Agents / Q000008:administration & dosage / Q000627:therapeutic use*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D042461:Vascular Endothelial Growth Factor A; D014665:Vasodilator Agents; D008315:Malondialdehyde; D000068677:Sildenafil Citrate