title: Immunoreactivity and two-dimensional gel-electrophoresis characterization of Egyptian cobra venom proteome.
abstract: The first and second (two) dimensional gel electrophoresis has a broad protein resolution power. It was used to separate and identify cobra venom proteome. The importance of characterizing venom proteins contents from the Egyptian elapidae, specifically neurotoxins, is based on the need to produce effective anti-venom. About 30-55distinct protein spots were identified on silver stained two-dimensional gels. Around two-thirds of the venom proteins displayed low a molecular weight and a migration into hydrophobic side. The venoms from Naja haja and Naja nigricollus showed 45-55 spots, while Walternnesia aegyptia had less (31-37) spots. The commercial prepared polyclonal antivenom had a strong signal for anionic and cationic venom protein spots with molecular weight 20-115 kDa. However, it showed weak or non immunoreactivity toward anionic low molecular weight spots (2.5-15 kDa). These results suggest the need to change the immunization schedule to include low molecular weight toxin-proteomes as separate dose or sequester injection.
authors.affiliation: Department of Biological Science, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.
authors.forename: Hussein Abduelrahman
authors.initials: HA
authors.lastname: Almehdar
authors.affiliation: Department of Biological Science, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.
authors.forename: Mahmoud Abass
authors.initials: MA
authors.lastname: Adel-Sadek
authors.affiliation: Department of Biological Science, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia / Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City for Scientific Research and Technology Applications, New Borg EL-Arab Alexandria, Egypt.
authors.forename: Elrashdy Moustafa
authors.initials: EM
authors.lastname: Redwan
mesh_terms: D000818:Animals; D000997:Antivenins / Q000032:analysis / Q000276:immunology; D004546:Elapid Venoms / Q000737:chemistry* / Q000276:immunology; D017815:Elapidae; D015180:Electrophoresis, Gel, Two-Dimensional; D057927:Hydrophobic and Hydrophilic Interactions; D005779:Immunodiffusion; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D040901:Proteomics / Q000379:methods*
chemical_list: D000997:Antivenins; D004546:Elapid Venoms; D011506:Proteins