title: [Repeating the Platelia™ Aspergillus test in samples positive for serum galactomannan: Is it necessary?].
abstract: BACKGROUND
  The manufacturers of the Platelia™ Aspergillus test recommend retesting every sample with a positive result to galactomannan detection.
  
  
  OBJECTIVE
  To evaluate the repeated results of the Platelia™ Aspergillus test on initially positive samples, and their association with clinical and microbiological studies.
  
  
  METHODS
  A total of 150 positive sera belonging to 92 patients were studied. More than one positive sample was obtained from 31 (33.6%) patients. Nine patients were diagnosed with probable invasive aspergillosis and two with proven Fusarium oxysporum and Scedosporium prolificans invasive fungal infection, respectively.
  
  
  RESULTS
  Only 35 out of 150 (23.3%) sera remained positive upon test repetition. The average OD-index difference between the initially positive and subsequently negative samples was 0.76. Sera from 3 patients out of 9 diagnosed with probable invasive aspergillosis yielded negative results after repetition.
  
  
  CONCLUSIONS
  Our study shows poor reproducibility of the positive samples for the Platelia™ Aspergillus test. These results reinforce the need to evaluate a second sample and to consider simultaneously repeating the first positive serum to enhance the diagnosis of invasive fungal infection.
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Sanitaria Valdecilla (IDIVAL), Santander, Cantabria, España. Electronic address: mroiz@humv.es.
authors.forename: María Pía
authors.initials: MP
authors.lastname: Roiz
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Sanitaria Valdecilla (IDIVAL), Santander, Cantabria, España.
authors.forename: María del Sol
authors.initials: Mdel S
authors.lastname: García
authors.affiliation: Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Sanitaria Valdecilla (IDIVAL), Santander, Cantabria, España.
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
mesh_terms: D001228:Aspergillosis / Q000097:blood* / Q000382:microbiology*; D001230:Aspergillus / Q000302:isolation & purification*; D003955:Diagnostic Tests, Routine; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008351:Mannans / Q000097:blood*; D009172:Mycology / Q000379:methods
chemical_list: D008351:Mannans; C012990:galactomannan; D005690:Galactose
keywords: Galactomanano; Galactomannan; Infección fúngica invasiva; Invasive fungal infection; Muestras positivas; Positive samples