title: Characteristic Fingerprint Based on Low Polar Constituents for Discrimination of Wolfiporia extensa according to Geographical Origin Using UV Spectroscopy and Chemometrics Methods.
abstract: The fungus species Wolfiporia extensa has a long history of medicinal usage and has also been commercially used to formulate nutraceuticals and functional foods in certain Asian countries. In the present study, a practical and promising method has been developed to discriminate the dried sclerotium of W. extensa collected from different geographical sites based on UV spectroscopy together with chemometrics methods. Characteristic fingerprint of low polar constituents of sample extracts that originated from chloroform has been obtained in the interval 250-400 nm. Chemometric pattern recognition methods such as partial least squares discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA) were applied to enhance the authenticity of discrimination of the specimens. The results showed that W. extensa samples were well classified according to their geographical origins. The proposed method can fully utilize diversified fingerprint characteristics of sclerotium of W. extensa and requires low-cost equipment and short-time analysis in comparison with other techniques. Meanwhile, this simple and efficient method may serve as a basis for the authentication of other medicinal fungi.
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China ; College of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
authors.forename: Ji
authors.identifier: 0000-0002-7737-0785
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Resources and Environment, Yuxi Normal University, Yuxi 653100, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China.
authors.forename: Yuanzhong
authors.identifier: 0000-0001-5376-757X
authors.initials: Y
authors.lastname: Wang