title: Cell response to single-walled carbon nanotubes in hybrid porous collagen sponges.
abstract: Three-dimensional (3D) porous collagen sponges incorporated with single-walled carbon nanotubes (SWCNTs) were prepared and used for 3D culture of bovine articular chondrocytes (BACs). The pore structures of the sponges were controlled by using ice particulates as a porogen material. The responses of cells to SWCNTs were investigated in this 3D cell culture system by evaluation of cell functions and cellular uptake of SWCNTs. The results showed that cells adhered and spatially distributed in the porous sponges. The incorporation of SWCNTs in the porous sponges promoted cell proliferation and production of sulfated glycosaminoglycans (sGAG). Confocal Raman imaging revealed that SWCNTs could be internalized by cells. The hybrid porous sponges not only provided nanostructured pore surfaces to facilitate cell proliferation and extracellular matrix (ECM) secretion but also supplied nanomaterials for cellular uptake which may be useful for biomedical applications.
authors.affiliation: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: MAO.Hongli@nims.go.jp.
authors.forename: Hongli
authors.initials: H
authors.lastname: Mao
authors.affiliation: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. Electronic address: KAWAZOE.Naoki@nims.go.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kawazoe
authors.affiliation: Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan; Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: Guoping.CHEN@nims.go.jp.
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010316:Particle Size; D011161:Porifera / Q000166:cytology*; D016062:Porosity; D013499:Surface Properties
chemical_list: D037742:Nanotubes, Carbon; D003094:Collagen
keywords: Cell functions; Cellular uptake; Porous sponges; Single-walled carbon nanotubes; Topography