title: Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR.
abstract: We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, 37% fewer RT-PCR tests are needed.
authors.affiliation: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD198, Inserm 1095, Marseille, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Edouard
authors.affiliation: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD198, Inserm 1095, Marseille, France.
authors.forename: Elsa
authors.initials: E
authors.lastname: Prudent
authors.affiliation: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD198, Inserm 1095, Marseille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gautret
authors.affiliation: Saudi Ministry of Health, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Ziad A
authors.initials: ZA
authors.lastname: Memish
authors.affiliation: Aix Marseille Université, URMITE, UM63, CNRS 7278, IRD198, Inserm 1095, Marseille, France didier.raoult@gmail.com.
authors.forename: Didier
authors.initials: D
authors.lastname: Raoult
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D003365:Costs and Cost Analysis; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification; D005612:Freeze Drying / Q000191:economics / Q000379:methods; D006801:Humans; D009305:Nasopharynx / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000191:economics / Q000379:methods*; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000191:economics / Q000379:methods*
chemical_list: D004269:DNA, Bacterial