title: Ethanol modulation of mammalian BK channels in excitable tissues: molecular targets and their possible contribution to alcohol-induced altered behavior.
abstract: In most tissues, the function of Ca(2+)- and voltage-gated K(+) (BK) channels is modified in response to ethanol concentrations reached in human blood during alcohol intoxication. In general, modification of BK current from ethanol-naïve preparations in response to brief ethanol exposure results from changes in channel open probability without modification of unitary conductance or change in BK protein levels in the membrane. Protracted and/or repeated ethanol exposure, however, may evoke changes in BK expression. The final ethanol effect on BK open probability leading to either BK current potentiation or BK current reduction is determined by an orchestration of molecular factors, including levels of activating ligand (Ca(2+) i), BK subunit composition and post-translational modifications, and the channel's lipid microenvironment. These factors seem to allosterically regulate a direct interaction between ethanol and a recognition pocket of discrete dimensions recently mapped to the channel-forming (slo1) subunit. Type of ethanol exposure also plays a role in the final BK response to the drug: in several central nervous system regions (e.g., striatum, primary sensory neurons, and supraoptic nucleus), acute exposure to ethanol reduces neuronal excitability by enhancing BK activity. In contrast, protracted or repetitive ethanol administration may alter BK subunit composition and membrane expression, rendering the BK complex insensitive to further ethanol exposure. In neurohypophyseal axon terminals, ethanol potentiation of BK channel activity leads to a reduction in neuropeptide release. In vascular smooth muscle, however, ethanol inhibition of BK current leads to cell contraction and vascular constriction.
authors.affiliation: Department of Pharmacology, College of Medicine, The University of Tennessee Health Science Center Memphis, TN, USA.
authors.forename: Alex M
authors.initials: AM
authors.lastname: Dopico
authors.affiliation: Department of Pharmacology, College of Medicine, The University of Tennessee Health Science Center Memphis, TN, USA.
authors.forename: Anna N
authors.initials: AN
authors.lastname: Bukiya
authors.affiliation: Department of Psychiatry, The University of Massachusetts Medical School Worcester, MA, USA.
authors.forename: Gilles E
authors.initials: GE
authors.lastname: Martin
keywords: BK beta subunits; alcohol tolerance; ethanol-recognition site; ion channels; membrane lipids; n-alkanols; slo1 proteins