title: Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide.
abstract: Extensive research indicates that graphene oxide (GO) can effectively deliver photosensitives (PSs) by π-π stacking for photodynamic therapy (PDT). However, due to the tight complexes of GO and PSs, the fluorescence of PSs are often drastically quenched via an energy/charge transfer process, which limits GO-PS systems for photodiagnostics especially in fluorescence imaging. To solve this problem, we herein strategically designed and prepared a novel photo-theranostic agent based on sinoporphyrin sodium (DVDMS) loaded PEGylated GO (GO-PEG-DVDMS) with improved fluorescence property for enhanced optical imaging guided PDT. The fluorescence of loaded DVDMS is drastically enhanced via intramolecular charge transfer. Meanwhile, the GO-PEG vehicles can significantly increase the tumor accumulation efficiency of DVDMS and lead to an improved PDT efficacy as compared to DVDMS alone. The cancer theranostic capability of the as-prepared GO-PEG-DVDMS was carefully investigated both in vitro and in vivo. Most intriguingly, 100% in vivo tumor elimination was achieved by intravenous injection of GO-PEG-DVDMS (2 mg/kg of DVDMS, 50 J) without tumor recurrence, loss of body weight or other noticeable toxicity. This novel GO-PEG-DVDMS theranostics is well suited for enhanced fluorescence imaging guided PDT.
authors.affiliation: Department of Radiology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China; National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States; Molecular Imaging Center of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yan
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States.
authors.forename: Gang
authors.initials: G
authors.lastname: Niu
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Lin
authors.affiliation: Laboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD 20892, United States.
authors.forename: Albert J
authors.initials: AJ
authors.lastname: Jin
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States.
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Key Laboratory of Magnetic Materials and Devices, & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Magnetic Materials and Devices, & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, Zhejiang 315201, China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wu
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: Jiangxi Qinglong Group Co., Ltd., No. 283 Dongfeng Street, Yichun, Jiangxi 336000, China.
authors.forename: Shaoliang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States. Electronic address: peng.huang@nih.gov.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Radiology, The Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, China; Molecular Imaging Center of Harbin Medical University, Harbin, Heilongjiang, China. Electronic address: shenbzh@vip.sina.com.
authors.forename: Baozhong
authors.initials: B
authors.lastname: Shen
authors.affiliation: National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), 31 Center Drive, Bethesda, MD 20892, United States. Electronic address: shawn.chen@nih.gov.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003952:Diagnostic Imaging; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D006108:Graphite / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008819:Mice, Nude; D018625:Microscopy, Atomic Force; D010087:Oxides / Q000138:chemical synthesis / Q000737:chemistry*; D010778:Photochemotherapy; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use*; D026082:Singlet Oxygen / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D014018:Tissue Distribution / Q000187:drug effects
chemical_list: D010087:Oxides; D011166:Porphyrins; C586896:sinoporphyrin sodium; D026082:Singlet Oxygen; D011092:Polyethylene Glycols; D006108:Graphite
keywords: Fluorescence imaging; Graphene oxide (GO); Photodynamic therapy; Photosensitizer; Sinoporphyrin sodium (DVDMS)