title: Comprehensive analysis of the mouse brain proteome sampled in mass spectrometry imaging.
abstract: On-tissue enzymatic digestion is performed in mass spectrometry imaging (MSI) experiments to access larger proteins and to assign protein identities. Most on-tissue digestion MSI studies have focused on method development rather than identifying the molecular features observed. Herein, we report a comprehensive study of the mouse brain proteome sampled by MSI. Using complementary proteases, we were able to identify 5337 peptides in the matrix-assisted laser desorption/ionization (MALDI) matrix, corresponding to 1198 proteins. 630 of these peptides, corresponding to 280 proteins, could be assigned to peaks in MSI data sets. Gene ontology and pathway analyses revealed that many of the proteins are involved in neurodegenerative disorders, such as Alzheimer's, Parkinson's, and Huntington's disease.
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, 2333ZA The Netherlands.
authors.forename: Bram
authors.initials: B
authors.lastname: Heijs
authors.forename: Ricardo J
authors.initials: RJ
authors.lastname: Carreira
authors.forename: Else A
authors.initials: EA
authors.lastname: Tolner
authors.forename: Arnoud H
authors.initials: AH
authors.lastname: de Ru
authors.forename: Arn M J M
authors.initials: AM
authors.lastname: van den Maagdenberg
authors.forename: Peter A
authors.initials: PA
authors.lastname: van Veelen
authors.forename: Liam A
authors.initials: LA
authors.lastname: McDonnell
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019636:Neurodegenerative Diseases / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D020543:Proteome