title: Spherically-clustered porous Au-Ag alloy nanoparticle prepared by partial inhibition of galvanic replacement and its application for efficient multimodal therapy.
abstract: The polyvinylpyrrolidone (PVP)-coated spherically clustered porous gold-silver alloy nanoparticle (PVP-SPAN) was prepared by low temperature mediated, partially inhibited galvanic replacement reaction followed by silver etching process. The prepared porous nanostructures exhibited excellent photothermal conversion efficiency under irradiation of near-infrared light (NIR) and allowed a high payload of both doxorubicin (Dox) and thiolated dye-labeled oligonucleotide, DNAzyme (FDz). Especially, PVP-SPAN provided 10 times higher loading capacity for oligonucleotide than conventional hollow nanoshells due to increased pore diameter and surface-to-volume ratio. We demonstrated highly efficient chemo-thermo-gene multitherapy based on codelivery of Dox and FDz with NIR-mediated photothermal therapeutic effect using a model system of hepatitis C virus infected human liver cells (Huh7 human hepatocarcinoma cell line containing hepatitis C virus NS3 gene replicon) compared to conventional hollow nanoshells.
authors.affiliation: Department of Chemistry, Center for RNA Research, Institute for Basic Sciences (IBS), Seoul National University, Seoul, 151-747, Republic of Korea.
authors.forename: Hongje
authors.initials: H
authors.lastname: Jang
authors.affiliation: Department of Chemistry, Center for RNA Research, Institute for Basic Sciences (IBS), Seoul National University, Seoul, 151-747, Republic of Korea.
authors.forename: Dal-Hee
authors.initials: DH
authors.lastname: Min
mesh_terms: D000497:Alloys / Q000737:chemistry*; D045744:Cell Line, Tumor; D003131:Combined Modality Therapy; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006046:Gold / Q000737:chemistry*; D016174:Hepacivirus / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D007259:Infrared Rays; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D036103:Nanotechnology / Q000379:methods*; D016062:Porosity; D011205:Povidone / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013696:Temperature; D017361:Viral Nonstructural Proteins / Q000172:deficiency / Q000235:genetics; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000497:Alloys; D021881:DNA, Catalytic; D004317:Doxorubicin; D004337:Drug Carriers; D006046:Gold; D006861:Hydrogen Peroxide; D011205:Povidone; D012834:Silver; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: drug delivery; galvanic replacement; hepatitis C; nanomaterials; photothermal therapy