title: [Establishment of a method for detecting peripheral blood circulating brain microvascular endothelial cells, a novel biomarker for blood-brain barrier injury].
abstract: OBJECTIVE
  To establish a method for detecting circulating brain microvascular endothelial cells (cBMECs), a novel biomarker of blood-brain barrier (BBB) injury.
  
  
  METHODS
  Blood samples were collected from 33 patients with AIDS encephalitis and 13 healthy subjects for detection of cBMECs, cECs and EPCs using magnetic affinity isolation and immune identification technology.
  
  
  RESULTS
  The numbers of cBMECs, cECs and EPCs were significantly higher in the AIDS patients than in the control subjects (t=4.298, P<0.01; t=4.886, P<0.01; t=4.889, P<0.01). An significant association was also noted between HIV load and cBMEC number (r=0.928, P<0.01).
  
  
  CONCLUSIONS
  We have successfully established a method for detecting peripheral blood cBMECs, which can be of important value in non-invasive assessment of BBB injury.
authors.affiliation: Department of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China.E-mail: lossier@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Du
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Dexi
authors.initials: D
authors.lastname: Chen
authors.forename: Jiawen
authors.initials: J
authors.lastname: Qiu
authors.forename: Xiaolong
authors.initials: X
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.forename: Shenghe
authors.initials: S
authors.lastname: Huang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000503:physiopathology; D015415:Biomarkers; D001812:Blood-Brain Barrier / Q000473:pathology*; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans
chemical_list: D015415:Biomarkers