title: Effects of soyasaponin I and soyasaponins-rich extract on the alternariol-induced cytotoxicity on Caco-2 cells.
abstract: Alternariol (AOH) is a mycotoxin produced by Alternaria spp. Soyasaponin I (Ss-I) is present naturally in legumes, and it has antioxidant properties. Cytotoxic and genotoxic effects of AOH have been demonstrated previously in vitro. In the present study, the cytotoxicity of AOH, Ss-I, and soyasaponins-rich extract from lentils was investigated; as well as, the cytoprotective effects of Ss-I and lentil extracts against AOH induced-cytotoxicity on Caco-2 cells. Cytotoxicity was carried out using MTT and PC assays (AOH: 3.125-100 µM, Ss-I: 3.125-50 µM, and lentil extracts: 1:0-1:32) during 24 h of exposure. Only AOH showed cytotoxic effect. The reduction in cell proliferation ranged from 25% to 47%. Simultaneous combination of Ss-I with AOH (1:1) increased cell proliferation (35%) compared to AOH tested alone. The Ss-I and extracts showed synergistic cytoprotective effects against cytotoxicity induced by AOH on Caco-2 cells. Food commodities containing Ss-I could contribute to diminish the toxicological risk that natural contaminant as AOH in diet can produce to humans.
authors.affiliation: Laboratory of Food Chemistry, School of Pharmacy, University of Camerino, Via S. Agostino 1, 62032 Camerino, Italy. Electronic address: pilar.viladonat@unicam.it.
authors.forename: Pilar
authors.initials: P
authors.lastname: Vila-Donat
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, Valencia 46100, Spain.
authors.forename: Celia
authors.initials: C
authors.lastname: Fernández-Blanco
authors.affiliation: Laboratory of Food Chemistry, School of Pharmacy, University of Camerino, Via S. Agostino 1, 62032 Camerino, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, Valencia 46100, Spain.
authors.forename: Guillermina
authors.initials: G
authors.lastname: Font
authors.affiliation: Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, Burjassot, Valencia 46100, Spain.
authors.forename: María-José
authors.initials: MJ
authors.lastname: Ruiz
mesh_terms: D000528:Alternaria; D000975:Antioxidants / Q000494:pharmacology; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019610:Cytoprotection; D004249:DNA Damage / Q000187:drug effects; D004357:Drug Synergism; D006801:Humans; D007783:Lactones / Q000633:toxicity*; D027806:Lens Plant / Q000737:chemistry; D009183:Mycotoxins / Q000633:toxicity; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D007783:Lactones; D009183:Mycotoxins; D010936:Plant Extracts; D012503:Saponins; C056684:soyasaponin I; D009828:Oleanolic Acid; C005197:alternariol
keywords: Alternariol; Caco-2 cells; Cytoprotective effect; Cytotoxicity; Soyasaponin I