title: Marinobacter hydrocarbonoclasticus NY-4, a novel denitrifying, moderately halophilic marine bacterium.
abstract: The isolation and characterization of a novel halophilic denitrifying marine bacterium is described. The halophilic bacterium, designated as NY-4, was isolated from soil in Yancheng City, China, and identified as Marinobacter hydrocarbonoclasticus by 16S rRNA gene sequence phylogenetic analysis. This organism can grow in NaCl concentrations ranging from 20 to 120 g/L. Optimum growth occurs at 80 g/L NaCl and pH 8.0. The organism can grow on a broad range of carbon sources and demonstrated efficient denitrifying ability (94.2% of nitrate removal and 80.9% of total nitrogen removal in 48 h). During denitrification by NY-4, no NO2 (-)-N was accumulated, N2 was the only gaseous product and no harmful N2O was produced. Because of its rapid denitrification ability, broad carbon use range and ability to grow under high salinity and pH conditions, NY-4 holds promise for the treatment of saline waste waters.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Rongpeng
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zi
authors.affiliation: Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian, 223300 People's Republic of China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Haofeng
authors.initials: H
authors.lastname: Gao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
keywords: 16S rRNA sequence; Denitrification; Marinobacter hydrocarbonoclasticus NY-4; Moderate halophile; Wastewater