title: Cell apoptosis and proliferation in rat brains after intracerebral hemorrhage: role of Wnt/β-catenin signaling pathway.
abstract: OBJECTIVE
  To investigate the role of Wnt/β-catenin signaling pathway in cell apoptosis and proliferation in intracerebral hemorrhage (ICH) in rats.
  
  
  METHODS
  The ICH rats were established by stereotaxic infusion of 50 µL autologous arterial blood into the right striatum. Pathological characteristics of brain tissue was assessed by hematoxylin and eosin and TUNEL staining, and the expressions of proliferating cell nuclear antigen (PCNA) and Wnt3a/β-catenin/cyclin D1 were investigated by immunohistochemistry or reverse-transcription polymerase chain reaction.
  
  
  RESULTS
  The number of apoptotic cells and PCNA-positive cells kept increasing from the day following the ICH induction and reached a peak on the 3rd and 14th days, respectively. Some of the PCNA-positive cells could coexpress neurofilament protein-200 or glial fibrillary acidic protein. Wnt3a, β-catenin, cyclin D1, and PCNA mRNAs reached maximal expression levels on the 3rd, 7th, 7th, and 14th days, respectively. Moreover, the number of apoptotic cells was significantly positively correlated with the expressions of Wnt3a and β-catenin mRNAs, and negatively correlated with the number of PCNA-positive cells.
  
  
  CONCLUSIONS
  Our results suggest that the Wnt/β-catenin signaling pathway is associated with cell apoptosis and expression of PCNA in the ICH rat brain and regulates the balance between cell apoptosis and proliferation.
authors.affiliation: Department of Endocrinology, First Affiliated Hospital, Luzhou Medical College, P.R. China. lyycx@foxmail.com
authors.forename: Ling
authors.initials: L
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.forename: Neng Bin
authors.initials: NB
authors.lastname: Chang
authors.forename: Ling
authors.initials: L
authors.lastname: Dou
authors.forename: Chao Xian
authors.initials: CX
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis; D049109:Cell Proliferation / Q000502:physiology; D002543:Cerebral Hemorrhage / Q000503:physiopathology*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley; D060449:Wnt Signaling Pathway / Q000502:physiology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen