title: Piper and Vismia species from Colombian Amazonia differentially affect cell proliferation of hepatocarcinoma cells.
abstract: There is an increasing interest to identify plant-derived natural products with antitumor activities. In this work, we have studied the effects of aqueous leaf extracts from Amazonian Vismia and Piper species on human hepatocarcinoma cell toxicity. Results showed that, depending on the cell type, the plants displayed differential effects; thus, Vismia baccifera induced the selective killing of HepG2, while increasing cell growth of PLC-PRF and SK-HEP-1. In contrast, these two last cell lines were sensitive to the toxicity by Piper krukoffii and Piper putumayoense, while the Piperaceae did not affect HepG2 growth. All the extracts induced cytotoxicity to rat hepatoma McA-RH7777, but were innocuous (V. baccifera at concentrations < 75 µg/mL) or even protected cells from basal death (P. putumayoense) in primary cultures of rat hepatocytes. In every case, cytotoxicity was accompanied by an intracellular accumulation of reactive oxygen species (ROS). These results provide evidence for the anticancer activities of the studied plants on specific cell lines and suggest that cell killing could be mediated by ROS, thus involving mechanisms independent of the plants free radical scavenging activities. Results also support the use of these extracts of the Vismia and Piper genera with opposite effects as a model system to study the mechanisms of the antitumoral activity against different types of hepatocarcinoma.
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. lizcanomvz@gmail.com.
authors.forename: Leandro J
authors.initials: LJ
authors.lastname: Lizcano
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. msg_@hotmail.com.
authors.forename: Maite
authors.initials: M
authors.lastname: Siles
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. jtrepiana001@ikasle.ehu.es.
authors.forename: Jenifer
authors.initials: J
authors.lastname: Trepiana
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. luisa.hernandez@ehu.es.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Hernández
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. rosaura.navarro@ehu.es.
authors.forename: Rosaura
authors.initials: R
authors.lastname: Navarro
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. mbego.ruizlarrea@ehu.es.
authors.forename: M Begoña
authors.initials: MB
authors.lastname: Ruiz-Larrea
authors.affiliation: Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain. joseignacio.ruizs@ehu.es.
authors.forename: José Ignacio
authors.initials: JI
authors.lastname: Ruiz-Sanz
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D059447:Cell Cycle Checkpoints; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D028084:Clusiaceae / Q000737:chemistry*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects; D006801:Humans; D008297:Male; D031701:Piper / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species