title: Gastric cancer in young vs old Romanian patients: immunoprofile with emphasis on maspin and mena protein reactivity.
abstract: Increasing number of early-onset gastric carcinomas (GCs) and controversial results regarding the differences among young and older patients with this type of cancer are the reasons why correlation of clinicopathological factors with molecular markers is necessary. The aim of our study was to compare the demographic, clinical and immunohistochemical (IHC) aspects in Romanian patients with GC diagnosed below and above 45 years old. In 191 samples provided from patients with GC, the clinicopathological parameters were correlated with a panel of 15 antibodies: E-cadherin, HER-2, VEGF, CD31, CD105, COX-2, maspin, bax, bcl-2, p53, Ki67, MLH-1, MSH-2, mena protein and vimentin. Compared to the conventional cases, GCs diagnosed below 45 years old were more frequently located at the gastroesophageal junction and presented a higher percentage of lymph node metastases. The diffuse type E-cadherin/mena/p53/Ki67/bax-negative cases that displayed nuclear maspin positivity were also more frequently in younger patients. The intestinal type early-onset GCs were the most angiogenic ones, the apoptotic rate being lower than in the intestinal type GCs of the aged. Compared to the conventional cases, in the early-onset GCs the nuclear maspin-mediated antiproliferative activity is more intense in diffuse type while the mena-dependent tumor cell proliferation is more characteristic for intestinal type GCs.
authors.affiliation: Department of Pathology, University of Medicine and Pharmacy, Tirgu-Mures, Romania.
authors.forename: Simona
authors.initials: S
authors.lastname: Gurzu
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Kadar
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
authors.forename: Tivadar
authors.initials: T
authors.lastname: Bara
authors.forename: Tivadar
authors.initials: T
authors.lastname: Bara
authors.forename: Ioana
authors.initials: I
authors.lastname: Halmaciu
authors.forename: Ioan
authors.initials: I
authors.lastname: Jung
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D016015:Logistic Models; D008198:Lymph Nodes / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D015999:Multivariate Analysis; D012383:Romania; D015843:Serpins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; C511939:Enah protein, human; D008840:Microfilament Proteins; C085344:SERPIN-B5; D015843:Serpins
keywords: Gastric cancer; angiogenesis; maspin; mena protein; microsatellite status