title: Study on the Mechanisms of Cartilage Tissue Damage Caused by Hydrogen Peroxide.
abstract: Hydrogen peroxide (H2O2), normally used in the prevention of local bacterial infection, is also used for clinical debridement in bone and joint surgery. Studies show that treatment with H2O2 can cause cartilage damage, leading to postoperative complications. H2O2 can induce apoptosis because of its strong oxidation activity. To investigate the molecular mechanisms of H2O2-induced chondrocyte apoptosis, the rat chondrocytes from the knee joint were used as a cell model for this study. The results showed that the H2O2-treated cells survived at a decreased rate, with apoptosis in a great number of chondrocytes. The expression of pro-apoptotic factors of chondrocytes, Bcl-2 and Bcl-xl, were downregulated, while the pro-apoptotic factor Bax upregulated. Two important factors ERK and p38 in MAPK signaling pathway were phosphorylated at a higher level, leading to apoptosis of chondrocytes. This study described the molecular mechanism of H2O2-induced chondrocyte apoptosis, providing a theoretical basis for the rational and clinical use of H2O2 as a disinfectant.
authors.affiliation: Department of Orthopedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.|Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Department of Orthopedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Shi Dong
authors.initials: SD
authors.lastname: Hong
authors.affiliation: Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Jing Rong
authors.initials: JR
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xin
authors.affiliation: Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Xuzhou 3rd Hospital, Affiliated Hospital of Jiangsu University, Xuzhou, China.
authors.forename: Xiao Wei
authors.initials: XW
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China. limingwang99@hotmail.com.
authors.forename: Li Ming
authors.initials: LM
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D002356:Cartilage / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism*; D004202:Disinfectants / Q000009:adverse effects / Q000633:toxicity*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000009:adverse effects / Q000633:toxicity*; D020935:MAP Kinase Signaling System; D051381:Rats; D017207:Rats, Sprague-Dawley; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D004202:Disinfectants; D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Apoptosis; Chondrocyte; Disinfection; H2O2; Joint surgery