title: Preliminary study on electrophysiological changes after cellular autograft in age-related macular degeneration.
abstract: Evolving atrophic macular degeneration represents at least 80% of all macular degenerations and is currently without a standardized care. Autologous fat transplantation efficacy was demonstrated by several studies, as these cells are able to produce growth factors. The aim of the work was to demonstrate possible therapeutic effect of the joined suprachoroidal graft of adipocytes, adipose-derived stem cells (ADSCs) in stromal vascular fractions (SVFs) of adipose tissue, and platelet-rich plasma (PRP). Twelve eyes in 12 dry age-related macular degeneration (AMD) patients, aged 71.25 (SD ± 6.8) between 62 and 80 years, were analyzed. A complete ocular evaluation was performed using best corrected visual acuity (BCVA), retinographic analysis, spectral-domain optical coherence tomography, microperimetry, computerized visual field, and standard electroretinogram (ERG). Each eye received a cell in graft between choroid and sclera of mature fat cells and ADSCs in SVF enriched with PRP by means of the variant second Limoli (Limoli retinal restoration technique [LRRT]). In order to test if the differences pre- and post-treatment were significant, the Wilcoxon signed-rank test has been performed. Adverse effects were not reported in the patients. After surgery with LRRT, the most significant increase in the ERG values was recorded by scotopic rod-ERG (answer coming from the rods), from 41.26 to 60.83 μV with an average increase of 47.44% highly significant (P < 0.05). Moderately significant was the one recorded by scotopic maximal ERG (answer coming from the rods and cones), from 112.22 to 129.68 μV with an average increase of 15.56% (P < 0.1). Cell-mediated therapy based on growth factors used appears interesting because it can improve the retinal functionality responses in the short term. The ERG could, therefore, be used to monitor the effect of cell-mediated regenerative therapies.
authors.affiliation: From the Low Vision Research of Milan (PGL, CL), Milan; Department of Ophthalmology (EMV), A. Fiorini Hospital, Terracina; Polo Pontino (EMV); CenterVue (MUM), Padova; and Department of Sense Organs (MN), Faculty of Medicine and Odontology, Sapienza University of Rome, Rome, Italy.
authors.forename: Paolo Giuseppe
authors.initials: PG
authors.lastname: Limoli
authors.forename: Enzo Maria
authors.initials: EM
authors.lastname: Vingolo
authors.forename: Marco Ulisses
authors.initials: MU
authors.lastname: Morales
authors.forename: Marcella
authors.initials: M
authors.lastname: Nebbioso
authors.forename: Celeste
authors.initials: C
authors.lastname: Limoli
mesh_terms: D017667:Adipocytes / Q000637:transplantation*; D000273:Adipose Tissue / Q000166:cytology / Q000637:transplantation*; D000368:Aged; D000369:Aged, 80 and over; D064592:Autografts; D002829:Choroid / Q000601:surgery*; D004596:Electroretinography; D006801:Humans; D008268:Macular Degeneration / Q000601:surgery*; D008875:Middle Aged; D053657:Platelet-Rich Plasma; D033581:Stem Cell Transplantation; D017154:Stromal Cells / Q000166:cytology