title: Pycnogenol® and its fractions influence the function of isolated heart in rats with experimental diabetes mellitus.
abstract: The aim of this study was to test the effect of Pycnogenol(®) (PYC) mixture and its three fractions (buthanolic, water, ethyl acetate) on heart function in rats with experimental diabetes mellitus (DM) and compare their effects to the diabetic group. Their antioxidant activity "in vitro" was also determined. DM rats (streptozotocin over 3 consecutive days at a dose of 25 mg/kg of body weight) had increased systolic blood pressure, thicker left ventriculi wall (LV) and weaker myocardial contraction, prolonged QT interval in comparison to controls rats. In comparison to the diabetic group, PYC (20 mg/kg b.w./day) suppressed the influence of DM on the LV, improved contraction, increased coronary flow and displayed negative effect on electrical activity of hearts. The most effective of PYC's fractions was the water fraction. It improved biometric parameters and hemodynamic function of the DM hearts, enhanced shortening the QT interval, reduced the amount of dysrhythmias of the DM hearts and had the strongest antioxidant activity. In conclusion, DM damaged isolated rat heart function. Only the water fraction improved the function of the diabetic heart. The different results of three fractions and PYC on myocardial function may be caused by a various lipo- and hydro-philic action of the PYC components.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Slovakia. Electronic address: kralova@fpharm.uniba.sk.
authors.forename: Eva
authors.initials: E
authors.lastname: Kralova
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Slovakia.
authors.forename: Stanislava
authors.initials: S
authors.lastname: Jankyova
authors.affiliation: Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Slovakia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Mucaji
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Slovakia.
authors.forename: Eva
authors.initials: E
authors.lastname: Gresakova
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Comenius University, Slovakia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Stankovicova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005419:Flavonoids / Q000008:administration & dosage / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D010936:Plant Extracts; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D002316:Cardiotonic Agents; D005419:Flavonoids; D010936:Plant Extracts; C024070:pycnogenols
keywords: Antioxidant activity; Diabetic rats; Fractions; Isolated heart; Pycnogenol(®)