title: miR-132/212 cluster inhibits the growth of lung cancer xenografts in nude mice.
abstract: OBJECTIVE
  Lung cancer remains the leading cause of cancer-related death worldwide and microRNAs (miRNAs) play important roles in lung cancer progression. In this study, we investigate the effects of miR-132/212 cluster on the growth of subcutaneous xenografts of human lung cancer H1299 cells in nude mice, and further explore the underlying mechanisms.
  
  
  METHODS
  Nude mice with subcutaneous transplantation tumor of human lung cancer H1299 cells were randomly divided into three groups: the sham group, the control vector group, and the microRNA-132/212 group. The control vector and microRNA-132/212 cluster plasmid was intratumoral injected respectively. Tumor volume was measured during the intervention process, with a tumor growth curve generated. Immunohistochemistry was performed to analyze the expression level of Ki-67, P21, CyclinD1 and CD31 in each group.
  
  
  RESULTS
  The tumor volume of miR-132/212 group was significantly smaller than that of the control group at the terminal time point (P < 0.05). The expression levels of Ki-67, CyclinD1 and CD31 in the miR-132/212 group was significantly lower than the control group (P < 0.05), while the expression levels of P21 in the miR-132/212 group were significantly higher than the control group (P < 0.05).
  
  
  CONCLUSIONS
  miR-132/212 cluster significantly inhibited the growth of subcutaneous xenografts of human lung cancer H1299 cells in nude mice. The inhibitory effect of miR-132/212 cluster in tumor growth may be mediated by upregulating the expression of P21 and downregulating the expression of CyclinD1, thereby inhibiting tumor tissue proliferation and angiogenesis and resulting in the inhibition of tumor growth.
authors.affiliation: Department of Radiotherapy, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Judong
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Radiotherapy, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Meng
authors.affiliation: Department of Radiotherapy, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Soochow University Suzhou 215123, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Wan
authors.affiliation: Department of Oncology, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Radiotherapy, Changzhou Tumor Hospital, Soochow University Changzhou 213001, China.
authors.forename: Xifa
authors.initials: X
authors.lastname: Zhou
keywords: CyclinD1; Lung cancer; P21; angiogenesis; miR-132/212 cluster