title: Development of a new separation media using ultra-thin glassy carbon film modified silica.
abstract: A self-polymerizable octatetrayne, 1,8-dialdehydebenzyl-1,3,5,7-octatetrayne, is synthesized and covalently attached to an amino-functionalized surface of silica particles. The silica particles with a monolayer coverage of octatetrayne were then thermally processed to various final temperatures of 200, 400 and 700°C. The amino-functionalization, covalent attachment of octatetrayne and thermal process of silica particles were monitored by scanning electron microscopy (SEM), infrared (IR) spectroscopy and thermogravimetric analysis (TGA). The thermally processed particles were then packed into a capillary column and evaluated as a stationary phase for HPLC. After chromatographic evaluation, the optimized temperature for thermal processing was determined to be 400°C, which provides the best modified silica particles SiO2-OCT-T400 with an ultra-thin glassy carbon film coating. The linear solvation energy relationship model indicated that the primary contributors in retention are dispersion and H-bond basicity. The application of SiO2-OCT-T400 as a stationary phase was further demonstrated by successful separation of nonpolar hydrocarbons mixture and a nucleosides mixture.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 100 W. 18th Avenue, Columbus, OH 43210, United States.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 100 W. 18th Avenue, Columbus, OH 43210, United States. Electronic address: olesik.1@osu.edu.
authors.forename: Susan V
authors.initials: SV
authors.lastname: Olesik
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D012822:Silicon Dioxide / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013818:Thermogravimetry
chemical_list: D002244:Carbon; D012822:Silicon Dioxide
keywords: Carbon stationary phase; Monolayer stationary phase; Self-assembly