title: DEVELOPMENTALLY REGULATED PLASMA MEMBRANE PROTEIN of Nicotiana benthamiana contributes to potyvirus movement and transports to plasmodesmata via the early secretory pathway and the actomyosin system.
abstract: The intercellular movement of plant viruses requires both viral and host proteins. Previous studies have demonstrated that the frame-shift protein P3N-PIPO (for the protein encoded by the open reading frame [ORF] containing 5'-terminus of P3 and a +2 frame-shift ORF called Pretty Interesting Potyviridae ORF and embedded in the P3) and CYLINDRICAL INCLUSION (CI) proteins were required for potyvirus cell-to-cell movement. Here, we provide genetic evidence showing that a Tobacco vein banding mosaic virus (TVBMV; genus Potyvirus) mutant carrying a truncated PIPO domain of 58 amino acid residues could move between cells and induce systemic infection in Nicotiana benthamiana plants; mutants carrying a PIPO domain of seven, 20, or 43 amino acid residues failed to move between cells and cause systemic infection in this host plant. Interestingly, the movement-defective mutants produced progeny that eliminated the previously introduced stop codons and thus restored their systemic movement ability. We also present evidence showing that a developmentally regulated plasma membrane protein of N. benthamiana (referred to as NbDREPP) interacted with both P3N-PIPO and CI of the movement-competent TVBMV. The knockdown of NbDREPP gene expression in N. benthamiana impeded the cell-to-cell movement of TVBMV. NbDREPP was shown to colocalize with TVBMV P3N-PIPO and CI at plasmodesmata (PD) and traffic to PD via the early secretory pathway and the actomyosin motility system. We also show that myosin XI-2 is specially required for transporting NbDREPP to PD. In conclusion, NbDREPP is a key host protein within the early secretory pathway and the actomyosin motility system that interacts with two movement proteins and influences virus movement.
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: Chao
authors.initials: C
authors.lastname: Geng
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Cong
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.) xdongli@sdau.edu.cn.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Li
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: An-Li
authors.initials: AL
authors.lastname: Mou
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: Rui
authors.initials: R
authors.lastname: Gao
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Liu
authors.affiliation: Laboratory of Plant Virology, Department of Plant Pathology, College of Plant Protection (C.G., Q.-Q.C., X.-D.L., A.-L.M., R.G., J.-L.L., Y.-P.T.), and Collaborative Innovation Centre for Annually High Yield and High Efficiency Production of Wheat and Corn (C.G., X.-D.L.), Shandong Agricultural University, Tai'an, Shandong 271018, China; andCollege of Plant Sciences, Jilin University, Changchun 130062, China (J.-L.L.).
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Tian
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism*; D000595:Amino Acid Sequence; D020126:Brefeldin A / Q000494:pharmacology; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D020868:Gene Silencing / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D008969:Molecular Sequence Data; D009068:Movement / Q000187:drug effects; D009154:Mutation; D009218:Myosins / Q000378:metabolism; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism*; D031425:Plasmodesmata / Q000187:drug effects / Q000378:metabolism*; D017800:Potyvirus / Q000472:pathogenicity; D011485:Protein Binding / Q000187:drug effects; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D055571:Secretory Pathway / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D053778:Thiazolidines / Q000494:pharmacology; D014026:Nicotiana / Q000187:drug effects / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D053778:Thiazolidines; D014764:Viral Proteins; D049452:Green Fluorescent Proteins; D020126:Brefeldin A; D000205:Actomyosin; D009218:Myosins; C037068:latrunculin B