title: Deoxynivalenol-induced cytokines and related genes in concanavalin A-stimulated primary chicken splenic lymphocytes.
abstract: Deoxynivalenol (DON) immunotoxicity and its induction of cytokines and related genes in the splenic lymphocytes of chickens have not been completely elucidated. In the present study, we aimed to evaluate the effects of 48 h of different DON treatments (0 μg/mL, 0.2 μg/mL, 0.8 μg/mL, 3.2 μg/mL, 12.5 μg/mL, and 50 μg/mL) on the secretion and the mRNA expressions of some cytokine genes, such as interleukin-1beta (IL-1β), IL-1RI, IL-2, IL-4, IL-6, IL-10, IL-12β, and IFN-γ in chicken splenic lymphocytes. The concentrations of IL-1RI, IL-6, IL-10, and IFN-γ were increased with the DON concentrations increasing (P<0.05 or P<0.01). However, the concentrations of IL-1β, IL-2, IL-4, and IL-12β were decreased with the DON concentrations increasing (P<0.05 or P<0.01). Except IL-1β, the mRNA expressions of the other cytokines were up-regulated by DON. The highest mRNA expressions values of IL-1RI, IL-4, IL-10, IL-12β, and IFN-γ were at 50 μg/mL DON treatment groups (P<0.05 or P<0.01), while the highest mRNA expressions values of IL-2 and IL-6 were at 12.5 μg/mL DON treatment groups (P<0.05 or P<0.01). Our data revealed that the potent effects of DON in affecting the secretion and the mRNA expression of the related cytokines in chicken splenic lymphocytes in vitro.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: zhihua_ren@126.com.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China; School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. Electronic address: wangyachao1688@126.com.
authors.forename: Yachao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: 847767028@qq.com.
authors.forename: Huidan
authors.initials: H
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: dengyt1012@hotmail.com.
authors.forename: Youtian
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: dengjl213@126.com.
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: zzcjl@126.com.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: wangyadudu@126.com.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: pengxi197313@163.com.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: cui580420@sicau.edu.cn.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: shenliuhong@sohu.com.
authors.forename: Liuhong
authors.initials: L
authors.lastname: Shen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: mxp886@sina.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China; Sichuan Province Key Laboratory of Animal Disease & Human Health, Ya'an 625014, China; Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Ya'an 625014, China. Electronic address: fangjing4109@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
mesh_terms: D000818:Animals; D002645:Chickens / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis*; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D061251:Primary Cell Culture; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D016207:Cytokines; D014255:Trichothecenes; D003208:Concanavalin A; C007262:deoxynivalenol
keywords: Chicken; Cytokines; Deoxynivalenol; Immunity; Mycotoxin