title: Characterization of glycerol phosphate oxidase from Streptococcus pneumoniae and its application for ketose synthesis.
abstract: Glycerol phosphate oxidase from Streptococcus pneumoniae (GPOS.pne) was purified and characterized. By the actions of GPOS.pne and dihydroxyacetone phosphate (DHAP)-dependent aldolases, various ketoses including rare sugars were synthesized with glyceraldehydes as acceptors in a one-pot four-enzyme system.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Zijie
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Division of Mathematics and Science, University of South Carolina Salkehatchie, Walterboro, SC 29488, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: hideki@jiangnan.edu.cn.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakanishi
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China. Electronic address: xdgao@jiangnan.edu.cn.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Gao
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000446:Aldehyde-Lyases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005985:Glyceraldehyde / Q000737:chemistry / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007661:Ketoses / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D013296:Streptococcus pneumoniae / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D007661:Ketoses; D011994:Recombinant Proteins; D005985:Glyceraldehyde; D005993:Glycerolphosphate Dehydrogenase; C036140:L-alpha-glycerol-phosphate oxidase; D002374:Catalase; D000135:Acid Phosphatase; D000446:Aldehyde-Lyases
keywords: Aldolase; Enzymatic synthesis; Glycerol phosphate oxidase; Rare sugar; Streptococcus pneumoniae