title: Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers.
abstract: This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5% relative to the resin mixture, % by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5% significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 µm and 15.87 µm, respectively. CH-5% had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1% had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.
authors.affiliation: Department of Restorative Dentistry, Graduate Operative and Preventive Dentistry, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana.|Department of Restorative Dentistry, Dental Biomaterials Division, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana.
authors.forename: María F
authors.initials: MF
authors.lastname: Hamilton
authors.affiliation: Department of Industrial and Physical Pharmacy, Purdue University, College of Pharmacy, West Lafayette, Indiana.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Otte
authors.affiliation: Department of Oral Biology, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gregory
authors.affiliation: Department of Industrial and Physical Pharmacy, Purdue University, College of Pharmacy, West Lafayette, Indiana.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Pinal
authors.affiliation: Department of Operative Dentistry, University of North Carolina, Chapel Hill, North Carolina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Ferreira-Zandoná
authors.affiliation: Department of Restorative Dentistry, Dental Biomaterials Division, Indiana University School of Dentistry (IUSD), Indianapolis, Indiana.
authors.forename: Marco C
authors.initials: MC
authors.lastname: Bottino
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010895:Pit and Fissure Sealants; D011108:Polymers; C009916:nylon 6; D002209:Caprolactam; D048271:Chitosan
keywords: antibacterial; chitosan; dental sealants; electrospinning; nylon-6; resin