title: Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line.
abstract: OBJECTIVE
  Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line.
  
  
  METHODS
  The alpha- and beta-amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography. The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 μg/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test.
  
  
  RESULTS
  The aLpha-amanitin showed a higher toxicity at 36 h, while the highest inhibition of protein synthesis by the beta-amanitin was observed at 24 h.
  
  
  CONCLUSIONS
  It was shown that the beta-amanitin may be responsible for toxicity, like alpha-amanitin, in Amanita phalloides mushroom poisoning. The early inhibition of protein synthesis for beta-amanitin might be useful for future experiments and research.
authors.forename: Ertuğrul
authors.initials: E
authors.lastname: Kaya
authors.forename: Recep
authors.initials: R
authors.lastname: Bayram
authors.forename: Kürşat Oğuz
authors.initials: KO
authors.lastname: Yaykaşli
authors.forename: İsmail
authors.initials: İ
authors.lastname: Yilmaz
authors.forename: Sait
authors.initials: S
authors.lastname: Bayram
authors.forename: Emine
authors.initials: E
authors.lastname: Yaykaşli
authors.forename: Muhsine Zeynep
authors.initials: MZ
authors.lastname: Yavuz
authors.forename: Ali Akçahan
authors.initials: AA
authors.lastname: Gepdıremen
mesh_terms: D053959:Alpha-Amanitin / Q000633:toxicity*; D000546:Amanitins / Q000633:toxicity*; D006801:Humans; D061986:MCF-7 Cells; D009145:Mushroom Poisoning
chemical_list: D053959:Alpha-Amanitin; D000546:Amanitins