title: A three-plane architectonic atlas of the rat hippocampal region.
abstract: The hippocampal region, comprising the hippocampal formation and the parahippocampal region, has been one of the most intensively studied parts of the brain for decades. Better understanding of its functional diversity and complexity has led to an increased demand for specificity in experimental procedures and manipulations. In view of the complex 3D structure of the hippocampal region, precisely positioned experimental approaches require a fine-grained architectural description that is available and readable to experimentalists lacking detailed anatomical experience. In this paper, we provide the first cyto- and chemoarchitectural description of the hippocampal formation and parahippocampal region in the rat at high resolution and in the three standard sectional planes: coronal, horizontal and sagittal. The atlas uses a series of adjacent sections stained for neurons and for a number of chemical marker substances, particularly parvalbumin and calbindin. All the borders defined in one plane have been cross-checked against their counterparts in the other two planes. The entire dataset will be made available as a web-based interactive application through the Rodent Brain WorkBench (http://www.rbwb.org) which, together with this paper, provides a unique atlas resource.
authors.affiliation: Centre for Neural Computation, Kavli Institute for System Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.|Institute of Science and Technology IST, Klosterneuburg, Austria.
authors.forename: Charlotte N
authors.initials: CN
authors.lastname: Boccara
authors.affiliation: Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Kjonigsen
authors.affiliation: Centre for Neural Computation, Kavli Institute for System Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Ingvild M
authors.initials: IM
authors.lastname: Hammer
authors.affiliation: Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
authors.forename: Jan G
authors.initials: JG
authors.lastname: Bjaalie
authors.affiliation: Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.
authors.forename: Trygve B
authors.initials: TB
authors.lastname: Leergaard
authors.affiliation: Centre for Neural Computation, Kavli Institute for System Neuroscience, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.
authors.forename: Menno P
authors.initials: MP
authors.lastname: Witter
mesh_terms: D000818:Animals; D001931:Brain Mapping; D005260:Female; D006624:Hippocampus / Q000033:anatomy & histology*; D021621:Imaging, Three-Dimensional; D008279:Magnetic Resonance Imaging; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D017208:Rats, Wistar
chemical_list: D009419:Nerve Tissue Proteins
keywords: 3D atlas; chemoarchitecture; cytoarchitecture; hippocampus; parahippocampal region