title: Effect of bone morphogenetic protein 7 on differentiation of adipose derived mesenchymal stem cells into brown adipocytes in rats.
abstract: OBJECTIVE
  To evaluate the effect of bone morphogenetic protein(BMP7)on the differentiation of adipose derived mesenchymal stem cells(AD-MSCs)isolated from different adipose tissues into brown adipocytes in rats.
  
  
  METHODS
  Primary AD-MSCs were isolated from rate interscapular brown adipose tissue(iBAT),inguinal subcutaneous white adipose tissue(sWAT),and epididymal white adipose tissue(eWAT),respectively,and then cultivated in vitro. Differentiation of AD-MSCs into brown adipocytes was induced by BMP7. The characteristics of brown adipocytes were detected by immunofluorescence staining and oil red staining of cells. The expression levels of brown adipocyte-related genes were detected by polymerase chain reaction.
  
  
  RESULTS
  AD-MSCs from iBAT and sWAT were differentiated into cluster multilocular cells,which were stained red by oil red "O"staining and showed uncoupling protein 1-positive by immunofluorescent staining method. AD-MSCs from eWAT had a small number of scattered multilocular cells and showed uncoupling protein 1-negative. The results of reverse transcription-polymerase chain reaction showed that the uncoupling protein 1 gene was highly expressed in the iBAT group and sWAT group but was negative in the eWAT group.
  
  
  CONCLUSIONS
  AD-MSCs isolated from different adipose tissues in rats have different gene expression profiles and differentiation potentials.
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Long
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Jun-Xia
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine,Xushui County People's Hospital,Xushui,Hebei 072550,China.
authors.forename: Min-Zhi
authors.initials: MZ
authors.lastname: Li
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Lian
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: Hebei Key Lab of Laboratory Animal Science,Hebei Medical University,Shijiazhuang 050017,China.
authors.forename: Shu-Feng
authors.initials: SF
authors.lastname: Liu
mesh_terms: D052437:Adipocytes, Brown / Q000502:physiology*; D000273:Adipose Tissue / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000502:physiology*; D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D007473:Ion Channels / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000378:metabolism; D009765:Obesity / Q000378:metabolism; D051381:Rats; D000071256:Uncoupling Protein 1
chemical_list: D055419:Bone Morphogenetic Protein 7; D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604373:Ucp1 protein, rat; D000071256:Uncoupling Protein 1