title: [Effects of 5-aza-2-deoxycytidine on methylation status of RECK gene and cancer cell invasion in tongue cancer SCC-4 cells].
abstract: OBJECTIVE
  To investigate the effects of 5-aza-2-deoxycytidine on methylation status and invasion ability of RECK gene in tongue cancer SCC-4 cells.
  
  
  METHODS
  Tongue cancer cell line SCC-4 cells were treated with 5-aza-dC at different concentrations for 72 h. Methylation status of RECK gene of SCC-4 cells was detected by methylation specific PCR (MSP), the expression of RECK gene mRNA was detected by real-time quantitative PCR. The expression of RECK protein was detected by Western blot, and the invasion ability of SCC-4 cell was examined by Transwell assay. SPSS13.0 software package was used for statistical analysis.
  
  
  RESULTS
  RECK gene of SCC-4 cells was in high methylation status in untreated group, abnormal methylation was effectively reversed by 5-aza-dC treatment. After treatment with different concentration of 5-aza-dC for 72 h, relative mRNA expression level increased gradually (P<0.05). The relative expression level of RECK protein in 5-aza-dC treated group was significantly higher than that in the control group,the invasion ability of SCC-4 cell was decreased gradually.
  
  
  CONCLUSIONS
  5-aza-dC treatment for tongue cancer SCC-4 cells can successfully reverse high methylation status of RECK gene and restore the expression of RECK gene mRNA and protein, and reduced the invasion ability.
authors.affiliation: Department of Prosthodontics, Stomatological Hospital of Jinan City. Jinan 250001, Shandong Province, China.E-mail:jiangxu63@163.com.
authors.forename: Xv
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000964:Antimetabolites, Antineoplastic; D001374:Azacitidine / Q000031:analogs & derivatives; D019175:DNA Methylation; D000077209:Decitabine; D058851:GPI-Linked Proteins / Q000235:genetics*; D006801:Humans; D008745:Methylation; D009361:Neoplasm Invasiveness; D012333:RNA, Messenger; D014062:Tongue Neoplasms
chemical_list: D000964:Antimetabolites, Antineoplastic; D058851:GPI-Linked Proteins; C115460:RECK protein, human; D012333:RNA, Messenger; D000077209:Decitabine; D001374:Azacitidine