title: High-level production of creatine amidinohydrolase from Arthrobacter nicotianae 23710 in Escherichia coli.
abstract: In the present study, the gene encoding creatinase was amplified from Arthrobacter nicotianae 23710 (CICC) and functionally overexpressed in Escherichia coli. By applying a two-stage temperature control strategy, the production of creatinase was increased up to 61.3 U/mL in 3-L fermentor with a high productivity of 6.1 U/mL/h. The recombinant creatinase shows excellent resistance to the chelating agent EDTA, the surfactants (Tween 20, Tween 80, and Triton X-100) and the common preservative NaN3 (20 mM). High-level expression of the recombinant creatinase will contribute to its application in clinical diagnosis of renal function.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.forename: Linpei
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Kang
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
mesh_terms: D001173:Arthrobacter / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D013696:Temperature; D014512:Ureohydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014512:Ureohydrolases; C019944:creatinase