title: Human peripheral blood CD34+ cells attenuate oleic acid-induced acute lung injury in rats.
abstract: OBJECTIVE
  Adult stem cell-based therapy is a promising novel approach for treatment of acute lung injury (ALI). In this study, we evaluated the therapeutic effect of isolated human peripheral blood CD34+ progenitor cells in an ALI rat model, induced by oleic acid (OA) injection.
  
  
  METHODS
  Seventy-five adult female rats were used in this study. Group A, control without treatment, and group B, control injected with phosphate-buffered saline, comprised 15 rats each; the remaining 45 rats were injected with OA to induce ALI and were further subdivided into 3 groups: group C (ALI group, 15 rats), group D (ALI and fibroblast group, 15 rats) and group E (ALI and CD34+ cell group, 15 rats).
  
  
  RESULTS
  CD34+ cells transplantation in rats with OA-induced lung injury improves the arterial PaO(2) and wet/dry ratio, reduces infiltration of inflammatory cells and decreases lung vascular permeability as determined by reduced intra-alveolar and interstitial patchy congestion and hemorrhage as well as decreased interstitial edema. Additionally, lung inflammation determined by expression of the pro-inflammatory mediators intercellular adhesion molecule 1 and tumor necrosis factor-α was attenuated in CD34+ cell-treated rats at 6, 24 and 48 h post-OA challenge compared with non-treated rats. Moreover, the expression of anti-inflammatory molecule interleukin-10 was up-regulated in the lung of OA-induced ALI rats after administration of CD34+ cells. The important finding was that human TNF-α-induced protein 6 (TSG-6) gene expression was significantly up-regulated in rats treated with CD34+ cells.
  
  
  CONCLUSIONS
  The freshly isolated human peripheral blood-derived CD34+ cells may be used as an important source of stem cells that improve ALI. The anti-inflammatory properties of CD34+ cells in the lung are explained, at least in part, by activation of CD34+ cells to express TSG-6.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. Electronic address: dr.somiahassan@yahoo.com.
authors.forename: Somia H
authors.initials: SH
authors.lastname: Abd-Allah
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Sally M
authors.initials: SM
authors.lastname: Shalaby
authors.affiliation: Department of Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Abd-Elbary
authors.affiliation: Department of Animal Wealth Development, Genetics and Genetic Engineering, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
authors.forename: Ayman A
authors.initials: AA
authors.lastname: Saleh
authors.affiliation: Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt. Electronic address: mohrizk73@yahoo.com.
authors.forename: Mohammed Abu
authors.initials: MA
authors.lastname: El-Magd
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000628:therapy*; D000328:Adult; D053687:Adult Stem Cells / Q000637:transplantation*; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002199:Capillary Permeability / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000628:therapy; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D008168:Lung / Q000473:pathology; D019301:Oleic Acid; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; C513056:Tnfaip6 protein, rat; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D019301:Oleic Acid
keywords: CD34(+)ve; ICAM-1; IL-10; TNF-α; TSG-6; acute lung injury; oleic acid