title: In situ mapping of the effect of additional mutations on starch granule structure in amylose-extender (ae) maize kernels.
abstract: Optical (KI/I2-staining, polarised) and FTIR microscopy has been used to monitor starch granule structure within wild-type (wt), GEMS-0067 and waxy-amylose-extender (wx-ae) maize mutant kernels. In the GEMS-0067 mutant containing the high amylose modifier (HAM) gene(s) plus the recessive ae gene, structural heterogeneity characteristic of the ae mutation was reduced markedly. However, enhanced variation in granule shape and size was observed distributed spatially within the kernel, which appears to be related to new heterogeneity in internal starch granule structure. In wx-ae starch mutants the ae gene led to heterogeneity of starch granule structure equivalent to that in single ae mutants, plus new structural heterogeneity coincident with novel induced variation in granule size and shape.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Food Research, Norwich NR4 7UA, UK.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Wellner
authors.affiliation: Institute of Food Research, Norwich NR4 7UA, UK.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Parker
authors.affiliation: Institute of Food Research, Norwich NR4 7UA, UK.
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Morris
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China. Electronic address: fcheng@zju.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Cheng
mesh_terms: D000688:Amylose / Q000737:chemistry*; D009154:Mutation; D017550:Spectroscopy, Fourier Transform Infrared; D013213:Starch / Q000737:chemistry; D003313:Zea mays / Q000737:chemistry*
chemical_list: D013213:Starch; D000688:Amylose
keywords: FTIR imaging; GEMS-0067; In situ starch granule structure; Optical microscopy; wx-ae maize mutants