title: Structure characterization and antitumor activity of a polysaccharide from the alkaline extract of king oyster mushroom.
abstract: A water-soluble polysaccharide, designated as KOMAP, was isolated and purified from the alkaline extract of king oyster mushroom, which was composed of glucose (Glc), mannose (Man) and arabinose (Ara) in a molar ratio of 6.2:2.1:2.0. It had an average molecular weight of 2.1×10(4)Da. GC-MS analysis revealed that KOMAP was a linear structure of the polymer with a backbone composed of β-1,4-linked glucopyranosyl and β-1,3,6-linked mannopyranosyl units, which was terminated with α-1-linked arabinofuranosyl unit at C-6 position of β-1,3,6-linked mannopyranosyl residue along the main chain in the ratio of 3.1:1. The results in the animal experiment showed that 50, 100 and 200mg/mL of KOMAP not only inhibited the tumor growth, but also increased relative thymus and spleen indices, LPS- or ConA-induced lymphocytes proliferation, and serum cytokine IL-2, TNF-α, and IFN-γ levels, as well as the activities of NK cells and CTLs in spleen of Renca tumor-bearing mice. In summary, our data indicate that the KOMAP exerts effective immunoregulatory and anti-tumor activities in vivo.
authors.affiliation: Department of Medical Records Management, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: liuxinkui@21cn.com.
authors.forename: Xinkui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Nursing Department, Henan Provincial People's Hospital, Zhengzhou 450003, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Respiratory, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450053, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides
keywords: Antitumor; Immunomodulatory; King oyster mushroom; Polysaccharide