title: Rapid and sensitive analysis of multiple bioactive constituents in tripterygium glycosides tablets using liquid chromatography coupled with time-of-flight mass spectrometry.
abstract: A simultaneous determination method based on liquid chromatography coupled with time-of-flight mass spectrometry was developed for the analysis of 11 bioactive constituents in tripterygium glycosides tablets, an immune and inflammatory prescription used in China. The analysis was fully optimized on a 1.8 μm particle size C18 column with linear gradient elution, permitting good separation of the 11 analytes and two internal standards in 21 min. The quantitation of each target constituent was carried out using the narrow window extracted ion chromatograms with a ±l0 ppm extraction window, yielding good linearity (r(2) > 0.996) with a linear range of 10-1000 ng/mL. The limits of quantitation were low ranging from 0.25 to 5.02 ng/mL for the 11 analytes, and the precisions and repeatability were better than 1.6 and 5.3%, respectively. The acceptable recoveries obtained were in the range of 93.4-107.4%. This proposed method was successfully applied to quantify the 11 bioactive constituents in commercial samples produced by nine pharmaceutical manufacturers to profile the quality of these preparations. The overall results demonstrate that the contents of the 11 bioactive constituents in different samples were in great diversity, therefore, the quality, clinical safety, and efficacy of this drug needs further research and evaluation.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, P. R. China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, P. R. China.
authors.forename: Meng-xiang
authors.initials: MX
authors.lastname: Su
authors.forename: Wen-di
authors.initials: WD
authors.lastname: Zhou
authors.forename: Juan
authors.initials: J
authors.lastname: Lan
authors.forename: Bin
authors.initials: B
authors.lastname: Di
authors.forename: Tai-jun
authors.initials: TJ
authors.lastname: Hang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D012680:Sensitivity and Specificity; D013607:Tablets / Q000737:chemistry; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D013607:Tablets
keywords: Liquid chromatography; Quantitative analysis; Time-of-flight mass spectrometry; Traditional Chinese medicine; Tripterygium wilfordii