title: The DNA-dependent protein kinase: A multifunctional protein kinase with roles in DNA double strand break repair and mitosis.
abstract: The DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase composed of a large catalytic subunit (DNA-PKcs) and the Ku70/80 heterodimer. Over the past two decades, significant progress has been made in elucidating the role of DNA-PK in non-homologous end joining (NHEJ), the major pathway for repair of ionizing radiation-induced DNA double strand breaks in human cells and recently, additional roles for DNA-PK have been reported. In this review, we will describe the biochemistry, structure and function of DNA-PK, its roles in DNA double strand break repair and its newly described roles in mitosis and other cellular processes.
authors.affiliation: Department of Biochemistry & Molecular Biology, Robson DNA Science Centre, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Jette
authors.affiliation: Department of Biochemistry & Molecular Biology, Robson DNA Science Centre, Southern Alberta Cancer Research Institute, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada. Electronic address: leesmill@ucalgary.ca.
authors.forename: Susan P
authors.initials: SP
authors.lastname: Lees-Miller
mesh_terms: D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair / Q000235:genetics*; D051747:DNA-Activated Protein Kinase / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D004789:Enzyme Activation; D006801:Humans; D008938:Mitosis / Q000235:genetics*; D008954:Models, Biological; D008956:Models, Chemical; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D011487:Protein Conformation
chemical_list: D004247:DNA; D051747:DNA-Activated Protein Kinase
keywords: DNA-dependent protein kinase; Mitosis; Non-homologous end joining