title: Predictive response surface model for heat-induced rheological changes and aggregation of whey protein concentrate.
abstract: Whey proteins are now far more than a by-product of cheese processing. In the last 2 decades, food manufacturers have developed them as ingredients, with the dairy industry remaining as a major user. For many applications, whey proteins are modified (denatured) to alter their structure and functional properties. The objective of this research was to study the influence of 85 to 100 °C, with protein concentration of 8% to 12%, and treatment times of 5 to 30 min, while measuring rheological properties (storage modulus, loss modulus, and complex viscosity) and aggregation (intermolecular beta-sheet formation) in dispersions of whey protein concentrate (WPC). A Box-Behnken Response Surface Methodology modeled the heat denaturation of liquid sweet WPC at 3 variables and 3 levels. The model revealed a very significant fit for viscoelastic properties, and a lesser fit for protein aggregation, at temperatures not previously studied. An exponential increase of rheological parameters was governed by protein concentration and temperature, while a modest linear relationship of aggregation was governed by temperature. Models such as these can serve as valuable guides to the ingredient and dairy industries to develop target products, as whey is a major ingredient in many functional foods.
authors.affiliation: W.K. Kellogg Inst, 2 Hamblin Ave. E, Battle Creek, MI, 49017, U.S.A.
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Alvarez
authors.forename: Charles
authors.initials: C
authors.lastname: Emond
authors.forename: Ahmed
authors.initials: A
authors.lastname: Gomaa
authors.forename: Gabriel E
authors.initials: GE
authors.lastname: Remondetto
authors.forename: Muriel
authors.initials: M
authors.lastname: Subirade
mesh_terms: D036262:Empirical Research; D005511:Food Handling; D006358:Hot Temperature; D008894:Milk Proteins / Q000737:chemistry*; D010316:Particle Size; D012212:Rheology; D017550:Spectroscopy, Fourier Transform Infrared; D014783:Viscosity; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D000067816:Whey Proteins
keywords: Box-Behnken model; Fourier transform infrared; dynamic rheology; heat processing; whey protein concentrate