title: Global transcriptomic profiling of Schizosaccharomyces pombe in response to nitrosative stress.
abstract: Excess production of nitric oxide (NO) and reactive nitrogen intermediates (RNIs) cause nitrosative stress on cells. Schizosaccharomyces pombe was used as a model to study nitrosative stress response. This is the first report on the global gene expression profile in response to NO in S. pombe using microarray. Among the 4824 genes reported for S. pombe, 818 were differentially expressed by at least 2-fold upon NO donor treatment. We previously showed that Pap1, the Activator Protein 1 transcription factor is required to combat nitrosative stress. In this study, the transcriptional response to NO in a null mutant for pap1 identified 45 genes that seem to be controlled by Pap1. Surprisingly, Pap1 regulated genes in S. pombe were distinctly different under nitrosative stress than those reported under oxidative stress. Genes of the pathway meiosis, cell cycle, spliceosome and oxidative phosphorylation were mostly affected under nitrosative stress in the fission yeast.
authors.affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, West Bengal, India.
authors.forename: Pranjal
authors.initials: P
authors.lastname: Biswas
authors.affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700 019, West Bengal, India. Electronic address: ghoshs71@hotmail.com.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D001483:Base Sequence; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics*; D000073718:Pancreatitis-Associated Proteins; D026361:Reactive Nitrogen Species / Q000378:metabolism / Q000633:toxicity; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D050976:Basic-Leucine Zipper Transcription Factors; D020030:Nitric Oxide Donors; D000073718:Pancreatitis-Associated Proteins; C105096:Pap1 protein, S pombe; C000612141:REG3A protein, human; D026361:Reactive Nitrogen Species; D029702:Schizosaccharomyces pombe Proteins; D009569:Nitric Oxide
keywords: Microarray analysis; Nitric oxide; Nitrosative stress; Schizosaccharomyces pombe