title: [Expression of phosphorylated caspase-8 in non-small cell lung cancer and the clinical implications].
abstract: OBJECTIVE
  To investigate the clinical significance of phosphorylated caspase-8 by Src in patients with operable non-small cell lung cancer.
  
  
  METHODS
  Src and caspase-8 expressions were tested using immunohistochemistry in non-small cell lung cancer tissues and control lung tissues. The expression of phosphorylated caspase-8 at 380 tyrosine by Src was detected using Western blotting. The disease-free survival (DFS) of patients positive and negative for phosphorylated caspase-8 was analyzed with Kaplan-Meire survival curve.
  
  
  RESULTS
  No significant difference was found in the positivity rate of caspase-8 or Src between the cancer tissues and control lung tissues (76.3% vs 83.3%, P>0.05; 70.1% vs 66.4%, P>0.05). All the patients with Casp8- and Src-positive cancers were positive for phosphoryalted caspase-8, whose expression rate was significantly higher in the cancer tissues than in the paired control lung tissues (52.4% vs 7.1%, P<0.05). The 2-year DFS was significantly higher in patients negative for phosphorylated caspase-8 than in the positive patients (32.0% vs 60.3%, P<0.05).
  
  
  CONCLUSIONS
  Phosphorylated caspase-8 may serve as a predictor for a poorer DFS in patients with operable non-small cell lung cancer.
authors.affiliation: Department of Oncology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.E-mail: mwl791218@126.com.
authors.forename: Weili
authors.initials: W
authors.lastname: Min
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Dai
authors.forename: Yinbin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xijing
authors.initials: X
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D053181:Caspase 8 / Q000378:metabolism*; D018572:Disease-Free Survival; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: C505530:CASP8 protein, human; D053181:Caspase 8