title: Antibacterial potential of rutin conjugated with thioglycolic acid capped cadmium telluride quantum dots (TGA-CdTe QDs).
abstract: Quantum dots not only act as nanocarrier but also act as stable and resistant natural fluorescent bio markers used in various in vitro and in vivo photolabelling and biological applications. In this study, the antimicrobial potential of TGA-CdTe QDs and commercial phenolics (rutin and caffeine) were investigated against Escherichiacoli. UV absorbance and fluorescence quenching study of TGA-CdTe QDs with rutin and caffeine complex was measured by spectroscopic technique. QDs-rutin conjugate exhibited excellent quenching property due to the -OH groups present in the rutin structure. But the same time caffeine has not conjugated with QDs because of lacking of -OH group in its structure. Photolabelling of E. coli with QDs-rutin and QDs-caffeine complex was analyzed by fluorescent microscopic method. Microbe E. coli cell membrane damage was assessed by atomic force (AFM) and confocal microscopy. Based on the results obtained, it is suggested that QDs-rutin conjugate enhance the antimicrobial activity more than the treatment with QDs, rutin and caffeine alone.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Devanesan Arul
authors.initials: DA
authors.lastname: Ananth
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India; TUV - SUD, South Asia Laboratory, Bangalore 560 058, India.
authors.forename: Angappan
authors.initials: A
authors.lastname: Rameshkumar
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Jeyadevi
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Sivanadanam
authors.initials: S
authors.lastname: Jagadeeswari
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Nagarajan
authors.affiliation: School of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
authors.forename: Rajalingam
authors.initials: R
authors.lastname: Renganathan
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: sudacoli@yahoo.com.
authors.forename: Thilagar
authors.initials: T
authors.lastname: Sivasudha
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D019187:Cadmium Compounds / Q000737:chemistry*; D002110:Caffeine / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D045663:Quantum Dots / Q000737:chemistry*; D012431:Rutin / Q000737:chemistry / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000737:chemistry*; D013864:Thioglycolates / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D019187:Cadmium Compounds; D013864:Thioglycolates; D002110:Caffeine; D012431:Rutin; C017487:2-mercaptoacetate; D013691:Tellurium; C028337:cadmium telluride
keywords: Antibacterial activity; Caffeine; Fluorescence quenching; Nanoparticles; Rutin; TGA-CdTe QDs