title: Combining serum and urine biomarkers in the early diagnosis of mild cognitive impairment that evolves into Alzheimer's disease in patients with the apolipoprotein E ϵ4 genotype.
abstract: Possession of the apolipoprotein E (APOE) ϵ4 genotype is a major predictor of progression to Alzheimer's disease (AD), particularly in patients with mild cognitive impairment (MCI). However, the use of APOE genotyping in the diagnosis of MCI is limited due to its low sensitivity and specificity, which often results in a high false-positive rate. In this study, we found that there was a significant decrease in serum BDNF and notable increase in urine AD7c-NTP in MCI patients who harbored the APOE ϵ4 allele. Both serum BDNF and urine AD7c-NTP had higher positive predictive values and were more sensitive biomarkers of MCI. Additionally, a testing strategy employing serum BDNF and urine AD7c-NTP revealed increases in sensitivity, positive and negative predictive values, and predictive ability compared with the use of either biomarker alone, suggested that combinatorial detection might have great potential for translation to the clinic.
authors.affiliation: Geriatric Key Laboratory, Hebei General Hospital , Shijiazhuang, Hebei , China .
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Cui
authors.forename: Jingci
authors.initials: J
authors.lastname: Yang
authors.forename: Jinrong
authors.initials: J
authors.lastname: Zhang
authors.forename: Dongcai
authors.initials: D
authors.lastname: Yuan
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.forename: Yali
authors.initials: Y
authors.lastname: Duo
authors.forename: Shengjun
authors.initials: S
authors.lastname: Li
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000652:urine; D053327:Apolipoprotein E4 / Q000235:genetics; D019208:Brain-Derived Neurotrophic Factor / Q000097:blood*; D016022:Case-Control Studies; D060825:Cognitive Dysfunction / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000652:urine; D018450:Disease Progression; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000652:urine*
chemical_list: C497612:AD7c-NTP protein, human; D053327:Apolipoprotein E4; D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; C579455:BDNF protein, human
keywords: Alzheimer’s disease; apolipoprotein E ϵ4; mild cognitive impairment; serum BDNF; urine AD7c-NTP