title: Induction of hsp70, hsp90, and catalase activity in planarian Dugesia japonica exposed to cadmium.
abstract: The hsp70 and hsp90 expression patterns and catalase (CAT) activity in the freshwater planaria Dugesia japonica exposed to cadmium (Cd) under laboratory conditions were investigated. Planaria were exposed to a range of Cd concentrations (0-150 μg Cd/L) for 24 h. The expression levels of hsp70 and hsp90 were determined by relative quantitative real-time polymerase chain reaction. Within the overall dose range in the experiment, the expression level of hsp70 and the activity of CAT in D. japonica were altered significantly. Hsp70 was induced in D. japonica upon Cd exposure concentrations as low as 9.375 μg Cd/L. No significant effect on the expression level of hsp90 was observed. Our findings demonstrated that stress gene hsp70, but not hsp90, was responsive to Cd contamination in D. japonica CAT activity was significantly induced at concentrations of 18.75, 37.5, and 75 μg Cd/L after 24-h exposure. We recommend that the use of hsp70 as a biomarker should be complemented by evidence of changes in other parameters, such as CAT activity, in D. japonica.
authors.affiliation: School of Life Sciences, Shandong University of Technology, Zibo, People's Republic of China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou, People's Republic of China.
authors.forename: Yehua
authors.initials: Y
authors.lastname: Mo
authors.affiliation: School of Life Sciences, Shandong University of Technology, Zibo, People's Republic of China.
authors.forename: Luming
authors.initials: L
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Shandong University of Technology, Zibo, People's Republic of China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Shandong University of Technology, Zibo, People's Republic of China.
authors.forename: Zhongchen
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Shandong University of Technology, Zibo, People's Republic of China zhxfshd@163.com.
authors.forename: Bosheng
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000737:chemistry / Q000378:metabolism*; D002681:China; D018840:HSP70 Heat-Shock Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D015801:Helminth Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000187:drug effects; D010932:Planarians / Q000187:drug effects* / Q000201:enzymology / Q000302:isolation & purification / Q000378:metabolism; D018510:RNA, Helminth / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D015801:Helminth Proteins; D018510:RNA, Helminth; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D002104:Cadmium; D002374:Catalase
keywords: RT-PCR; Stress genes; biomarker; cadmium; catalase; planarian (Dugesia japonica)