title: Insights into anti-parasitism induced by a C-type lectin from Bothrops pauloensis venom on Toxoplasma gondii.
abstract: Here we evaluate the effects of BpLec, a C-type lectin isolated from Bothrops pauloensis snake venom, on Toxoplasma gondii parasitism. BpLec (0.195-12.5 μg/mL) did not interfere with HeLa (host cell) viability by MTT assay, whereas higher doses decreased viability and changed HeLa morphology. In addition, the host cell treatment before infection did not influence adhesion and proliferation indexes. BpLec did not alter T. gondii tachyzoite viability, as carried out by trypan blue exclusion, but decreased both adhesion and parasite replication, when tachyzoites were treated before infection. Galactose (0.4 M) inhibited the BpLec effect on adhesion assays, suggesting that BpLec probably recognize some glycoconjugate from T. gondii membrane. Additionally, we performed cytokine measurements from supernatants collected from HeLa cells infected with T. gondii tachyzoites previously treated with RPMI or BpLec. MIF and IL-6 productions by HeLa cells were increased by BpLec treatment. Also, TGF-β1 secretion was diminished post-infection, although this effect was not dependent on BpLec treatment. Taken together, our results show that BpLec is capable of reducing T. gondii parasitism after tachyzoite treatment and may represent an interesting tool in the search for parasite antigens involved in these processes.
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil; INCT, Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica, Brazil.
authors.forename: Letícia
authors.initials: L
authors.lastname: Castanheira
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Dayane Lorena
authors.initials: DL
authors.lastname: Naves de Souza
authors.affiliation: Laboratório de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Rafaela José
authors.initials: RJ
authors.lastname: Silva
authors.affiliation: Laboratório de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Bellisa
authors.initials: B
authors.lastname: Barbosa
authors.affiliation: Laboratório de Imunoparasitologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: José Roberto
authors.initials: JR
authors.lastname: Mineo
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Kelly Aparecida
authors.initials: KA
authors.lastname: Tudini
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Rodrigues
authors.affiliation: Laboratório de Histologia e Embriologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil.
authors.forename: Eloísa Vieira
authors.initials: EV
authors.lastname: Ferro
authors.affiliation: Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil; INCT, Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica, Brazil. Electronic address: veridiana@ingeb.ufu.br.
authors.forename: Veridiana
authors.initials: V
authors.lastname: de Melo Rodrigues
mesh_terms: D000818:Animals; D017837:Bothrops / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003049:Coccidiostats / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D037181:Lectins, C-Type / Q000737:chemistry* / Q000302:isolation & purification; D014122:Toxoplasma / Q000187:drug effects*; D014688:Venoms / Q000737:chemistry*
chemical_list: D003049:Coccidiostats; D016207:Cytokines; D037181:Lectins, C-Type; D014688:Venoms
keywords: C-type lectin; Snake venom; Toxoplasma gondii