title: Nesfatin-1 suppresses gastric contractions and inhibits interdigestive migrating contractions in conscious dogs.
abstract: BACKGROUND
  Nesfatin-1 is a novel 82-amino acid anorectic peptide. Acute injection of nesfatin-1 into the third brain ventricle reduces food consumption during the dark phase in rats. Nesfatin-1 is also expressed in gastric X/A-like cells in the peripheral tissues. Nesfatin-1 has been reported to reduce gastric and duodenal motility and to delay gastric emptying.
  
  
  OBJECTIVE
  In the present study, we investigated the effects of nesfatin-1 on gastrointestinal motility in conscious dogs.
  
  
  METHODS
  Force transducers were implanted onto the serosal surfaces of the gastric bodies, gastric antra, duodena, and jejuna of healthy beagle dogs, and gastrointestinal motility was monitored. We evaluated the effects of nesfatin-1 on gastrointestinal motility and on the circulating levels of nesfatin-1 in the fasted and fed states.
  
  
  RESULTS
  The intravenous administration of nesfatin-1 reduced gastric contractions and inhibited cyclical interdigestive migrating contractions in the fasted state. In the fasted state, circulating levels of nesfatin-1 tended to increase during late phase I. In addition, the kinetics of the circulating levels of nesfatin-1 were opposite to those of ghrelin during the fasted state.
  
  
  CONCLUSIONS
  Nesfatin-1 regulates gastrointestinal motility, and, in particular, it inhibits gastric contractions in the fasted state. Interdigestive migrating contractions may be regulated by interactions between nesfatin-1, ghrelin, and motilin.
authors.affiliation: Department of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan, akira_watanabe@gunma-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Watanabe
authors.forename: Erito
authors.initials: E
authors.lastname: Mochiki
authors.forename: Akiharu
authors.initials: A
authors.lastname: Kimura
authors.forename: Norimichi
authors.initials: N
authors.lastname: Kogure
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Yanai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Toyomasu
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Ogata
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Ohno
authors.forename: Hideki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D002135:Calcium-Binding Proteins / Q000097:blood / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000097:blood / Q000494:pharmacology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005215:Fasting; D005769:Gastrointestinal Motility / Q000187:drug effects*; D054439:Ghrelin / Q000097:blood; D009037:Motilin / Q000097:blood; D009119:Muscle Contraction / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000097:blood / Q000494:pharmacology*; D000080866:Nucleobindins; D014159:Transducers
chemical_list: D015415:Biomarkers; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D054439:Ghrelin; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D009037:Motilin