title: Spectrophotometric determination of 4-acetamidophenyl N'-(sulphanilamide) acetate in biological fluids.
abstract: A simple, accurate and low cost spectrophotometric method is proposed for the determination of the synthesized paracetamol derivative; 4-acetamidophenyl N'-(sulphanilamide) acetate (APSA) in biological fluids. The spectrophotometric method is based on a condensation reaction between the alcoholic solution of APSA and acidic solution of p-dimethylaminobenzaldeyde (DPMK) to generate a yellow colored product. The linear range for the determination of APSA was 1-10 µg mL(-1) with molar absorptivity of 3.6877 × 10(4) L mol(-1) cm(-1) and Sandell's sensitivity of 0.001 µg cm-2/0.001 absorbance unit. During the inter-day and intra-day analysis, the relative standard deviation for replicated determination of APSA was found to be less than 2.0% and accuracy was 99.20-101.60% and 99.10-101.30% in blood and urine samples, respectively. There was no interference with commonly used blood and urine sample. The developed spectrophotometric method was successfully applied to assess APSA in biological fluids.
authors.forename: Bhavna
authors.initials: B
authors.lastname: Shah
authors.forename: Pravin
authors.initials: P
authors.lastname: Patil
authors.forename: Hirva
authors.initials: H
authors.lastname: Shah
mesh_terms: D000438:Alcohols / Q000737:chemistry; D000577:Amides / Q000097:blood* / Q000652:urine*; D001547:Benzaldehydes / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008956:Models, Chemical; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012997:Solvents; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013424:Sulfanilamides / Q000097:blood* / Q000652:urine*; D013696:Temperature
chemical_list: C000597867:4-acetamidophenyl N'-(sulfanilamide)acetate; D000438:Alcohols; D000577:Amides; D001547:Benzaldehydes; D012997:Solvents; D013424:Sulfanilamides; C510241:p-dimethylaminobenzaldehyde