title: Dendrofalconerol A suppresses migrating cancer cells via EMT and integrin proteins.
abstract: OBJECTIVE
  Enhanced cell motility is a hallmark of highly metastatic cancer cells. The anti-migratory activity of Dendrofalconerol A (DF-A), a pure bis(bibenzyls) isolated from the stem of Dendrobium falconeri (Orchidaceae) is reported in the present study.
  
  
  METHODS
  Cytotoxicity effects of DF-A on H460 lung cancer cells was determined by the MTT assay. We also investigated the mechanism of DF-A-mediated EMT and integrin proteins level by western blotting.
  
  
  RESULTS
  DF-A at concentrations of 0.5-5 μM significantly reduced the protein level of migrating cells in a dose-dependent manner. The expression of migration-related integrins, including integrin β1 and integrin α4 was significantly reduced in response to DF-A treatment. Also, DF-A was shown to suppress epithelial to mesenchymal transition (EMT), as indicated by cadherin switch from N- to E-cadherin and decrease of Snail, Slug and vimentin.
  
  
  CONCLUSIONS
  This study revealed the potential of DF-A, an anti-metastasis agent and the underlying mechanism in this in vitro assay with H460 cells, which leads to the development of a novel anti-metastatic agent.
authors.affiliation: Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Premkamol
authors.initials: P
authors.lastname: Pengpaeng
authors.affiliation: Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Boonchoo
authors.initials: B
authors.lastname: Sritularak
authors.affiliation: Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi.c@chula.ac.th pithi_chan@yahoo.com.
authors.forename: Pithi
authors.initials: P
authors.lastname: Chanvorachote
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001632:Bibenzyls / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D039423:Integrin alpha1 / Q000378:metabolism*; D039663:Integrin beta4 / Q000378:metabolism*; D014966:Xanthenes / Q000494:pharmacology*
chemical_list: C000595880:4,6-dimethoxy-9-(4-methoxybenzyl)-8-(2-(4-methoxyphenyl)ethyl)-9H-xanthene-2,3,5-triol; D000972:Antineoplastic Agents, Phytogenic; D001632:Bibenzyls; D039423:Integrin alpha1; D039663:Integrin beta4; D014966:Xanthenes
keywords: Dendrobium falconeri; EMT; dendrofalconerol A; integrin; lung cancer; migration; suppression