title: A transporter for abiotic stress and plant metabolite resistance in the ectomycorrhizal fungus Tricholoma vaccinum.
abstract: Fungi exposed to toxic substances including heavy metals, xenobiotics, or secondary metabolites formed by co-occurring plants or other microorganisms require a detoxification system provided by exporters of several classes of transmembrane proteins. In case of mycorrhiza, plant metabolites need to be exported at the plant interface, while the extraradical hyphae may prevent heavy metal uptake, thus acting as a biofilter to the host plant at high environmental concentrations. One major family of such transporter proteins is the multidrug and toxic compound extrusion (MATE) class, a member of which, Mte1, was studied in the ectomycorrhizal fungus Tricholoma vaccinum. Phylogenetic analyses placed the protein in a subgroup of basidiomycete MATE sequences. The gene mte1 was found to be induced during symbiotic interaction. It mediated detoxification of xenobiotics and metal ions such as Cu, Li, Al, and Ni, as well as secondary plant metabolites if heterologously expressed in Saccharomyces cerevisiae.
authors.affiliation: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany.
authors.forename: Ines
authors.initials: I
authors.lastname: Schlunk
authors.affiliation: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Krause
authors.affiliation: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany.
authors.forename: Sophia
authors.initials: S
authors.lastname: Wirth
authors.affiliation: Institute of Microbiology, Microbial Communication, Friedrich Schiller University, Neugasse 25, 07743, Jena, Germany. erika.kothe@uni-jena.de.
authors.forename: Erika
authors.initials: E
authors.lastname: Kothe
mesh_terms: D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D008658:Inactivation, Metabolic; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D019216:Metals, Heavy / Q000378:metabolism; D038821:Mycorrhizae / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D010944:Plants / Q000378:metabolism / Q000382:microbiology; D020539:Sequence Analysis, Protein; D013312:Stress, Physiological; D013559:Symbiosis; D055439:Tricholoma / Q000235:genetics / Q000378:metabolism*; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D019216:Metals, Heavy; D015262:Xenobiotics
keywords: Ectomycorrhiza; Metal resistance; Multidrug and toxic compound extrusion family (MATE); Plant metabolite detoxification; Tricholoma vaccinum