title: Evaluation of ploidy status using DNA-image cytometry of exfoliated mucosal cells in oral lichen planus.
abstract: BACKGROUND
  Oral lichen planus (OLP) is one of the potentially malignant disorders (PMDs) with a malignancy rate of 0.2-2%. Aneuploidy is considered to be one of the important markers for malignant transformation and DNA-image cytometry (DIC) has been successfully employed in oral mucosal PMDs and also in tumors of the cervix, lung and biliary tract.
  
  
  OBJECTIVE
  In this study, we intend to assess the ploidy status of exfoliated cells in OLP using DIC.
  
  
  METHODS
  Exfoliated cells from 48 patients with different subtypes of OLP (reticular, plaque type, erosive and atrophic) and 10 controls were stained using Feulgen reaction and assessed for integrated optical density using image analysis software and the ploidy status was assessed.
  
  
  RESULTS
  All the patients in the control group and most of the patients (93.5%) who had reticular or plaque type of OLP (29 out of 31) exhibited diploid nuclei in the smears, whereas 11 patients who had erosive or atrophic types of OLP showed aneuploid nuclei.
  
  
  CONCLUSIONS
  The patients with erosive or atrophic types of OLP are at more risk and assessment of ploidy status by exfoliative cytology can be used as an adjuvant for diagnosis.
authors.affiliation: Department of Oral Pathology, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: Ravi Teja
authors.initials: RT
authors.lastname: Chitturi
authors.affiliation: Department of Oral Pathology, Rajah Muthiah Dental College and Hospital, Cuddalore, Tamil Nadu, India.
authors.forename: R Madhavan
authors.initials: RM
authors.lastname: Nirmal
authors.affiliation: Department of Oral Pathology, Sree Anjaneya Institute of Dental Sciences, Calicut, Kerala, India.
authors.forename: Paramel Mohan
authors.initials: PM
authors.lastname: Sunil
authors.affiliation: Department of Oral Pathology, Indira Gandhi Institute of Dental Sciences, Villupuram, India.
authors.forename: A Santha
authors.initials: AS
authors.lastname: Devy
authors.affiliation: Department of Oral Pathology, SIBAR Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
authors.forename: B Venkat Ramana
authors.initials: BV
authors.lastname: Reddy
keywords: Cytology; DNA-image cytometry; integrated optical density; oral lichen planus