title: Dendritic glycopolymers as dynamic and covalent coating in capillary electrophoresis: view on protein separation processes and detection of nanogram-scaled albumin in biological samples.
abstract: Unique properties of dendritic polymers make them promising candidates for application as additives in various analytical methods. From this point, we investigated the potential use of maltose-modified hyperbranched poly(ethylene imine) (PEI-Mal) as a dynamically or covalently bound coating and a pseudostationary phase in capillary electrophoresis. The EOF mobilities were measured at different pH values (2.2, 8.5, and 10.2) using PEI-Mal in the background electrolyte (BGE) and desirable repeatability of the EOF with % RSD (n=50) ≤3.3 was obtained. The influence of pH, polymer concentration, and density of maltose shell on the separation properties of a model mixture of four proteins (albumin, lysozyme, myoglobin, insulin) were investigated. Applying PEI-Mal as a dynamic coating, improved separation of the protein mixture with a high repeatability was achieved. Applying PEI-Mal as a covalent coating for concentrating proteins in the large volume sample stacking (LVSS) combined with the field-enhanced sample injection (FESI), up to 1320-fold enhancement of sensitivity was achieved. The detection limit of 100-500ng/ml allowed successful analysis of albumin level both in blood and urine samples without additional preconcentration.
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany; Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.
authors.forename: Nikita
authors.initials: N
authors.lastname: Polikarpov
authors.affiliation: Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia.
authors.forename: Vera
authors.initials: V
authors.lastname: Potolytsyna
authors.affiliation: Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: bessonova.elena.a@gmail.com.
authors.forename: Elena
authors.initials: E
authors.lastname: Bessonova
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tripp
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1).
authors.forename: Dietmar
authors.initials: D
authors.lastname: Appelhans
authors.affiliation: Leibniz Institute of Polymer Research Dresden, Hohe Straße 6, D-01069 Dresden, Germany(1); Organic Chemistry of Polymers, Technische Universität Dresden, D-01062 Dresden, Germany.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Voit
authors.affiliation: Organic Chemistry Department, Saint Petersburg State University, Universitetsky Prospekt 26, Peterhof, 198504 Saint Petersburg, Russia. Electronic address: kartsova@gmail.com.
authors.forename: Ludmilla
authors.initials: L
authors.lastname: Kartsova
mesh_terms: D000418:Albumins / Q000032:analysis*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004573:Electrolytes / Q000737:chemistry; D019075:Electrophoresis, Capillary; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry
chemical_list: D000418:Albumins; D004573:Electrolytes; D011108:Polymers; D011506:Proteins
keywords: Capillary electrophoresis; Covalent coating; Dynamic coating; Hyperbranched polymers; On-line concentration; Proteins