title: Stereospecific recognition and quantitative structure-activity relationship between antibodies and enantiomers: ofloxacin as a model hapten.
abstract: In this study, ofloxacin stereoisomers were chosen as a simple model to investigate the stereospecific recognition of chiral haptens and antibodies. Three polyclonal antibodies were studied and showed a relatively high enantioselectivity and an excellent sensitivity. Comparative molecular field analysis and comparative molecular similarity indices analysis were employed to investigate the chiral recognition between the antibody and the ofloxacin enantiomer, and all the models yielded high correlation and predictive ability. It was found that the chiral discrimination was probably caused by steric hindrance; the antibody stereospecificity could be ascribed to the variation of the R1 and R3 groups of quinolones; the common structure of the quinolones is also essential in the hapten-antibody recognition. The recognition between the chiral haptens and the antibodies was co-affected by multiple interaction forces, and those forces were defined explicitly at the sub-structural level. An illustrative enhanced model with good simplicity and universality was also developed for a better understanding of the stereospecific recognition of ofloxacin enantiomers and antibodies for the first time. This work provides insights into the stereospecific recognition of chiral haptens and antibodies.
authors.affiliation: Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University/Guangdong Provincial Engineering & Technique Research Centre of Food Safety Detection and Risk Assessment, Guangzhou 510642, China. immunoassay@126.com.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Mu
authors.forename: Baoling
authors.initials: B
authors.lastname: Wang
authors.forename: Zhenlin
authors.initials: Z
authors.lastname: Xu
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Sun
authors.forename: Xinan
authors.initials: X
authors.lastname: Huang
authors.forename: Yudong
authors.initials: Y
authors.lastname: Shen
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Zherdev
authors.forename: Boris B
authors.initials: BB
authors.lastname: Dzantiev
authors.forename: Hongtao
authors.initials: H
authors.lastname: Lei
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D008958:Models, Molecular; D015242:Ofloxacin / Q000737:chemistry / Q000276:immunology*; D021281:Quantitative Structure-Activity Relationship; D013237:Stereoisomerism
chemical_list: D000906:Antibodies; D006241:Haptens; D015242:Ofloxacin