title: Erythropoietin does not reduce plasma lactate, H⁺, and K⁺ during intense exercise.
abstract: It is investigated if recombinant human erythropoietin (rHuEPO) treatment for 15 weeks (n = 8) reduces extracellular accumulation of metabolic stress markers such as lactate, H(+) , and K(+) during incremental exhaustive exercise. After rHuEPO treatment, normalization of blood volume and composition by hemodilution preceded an additional incremental test. Group averages were calculated for an exercise intensity ∼80% of pre-rHuEPO peak power output. After rHuEPO treatment, leg lactate release to the plasma compartment was similar to before (4.3 ± 1.6 vs 3.9 ± 2.5 mmol/min) and remained similar after hemodilution. Venous lactate concentration was higher (P < 0.05) after rHuEPO treatment (7.1 ± 1.6 vs 5.2 ± 2.1 mM). Leg H(+) release to the plasma compartment after rHuEPO was similar to before (19.6 ± 5.4 vs 17.6 ± 6.0 mmol/min) and remained similar after hemodilution. Nevertheless, venous pH was lower (P < 0.05) after rHuEPO treatment (7.18 ± 0.04 vs 7.22 ± 0.05). Leg K(+) release to the plasma compartment after rHuEPO treatment was similar to before (0.8 ± 0.5 vs 0.7 ± 0.7 mmol/min) and remained similar after hemodilution. Additionally, venous K(+) concentrations were similar after vs before rHuEPO (5.3 ± 0.3 vs 5.1 ± 0.4 mM). In conclusion, rHuEPO does not reduce plasma accumulation of lactate, H(+) , and K(+) at work rates corresponding to ∼80% of peak power output.
authors.affiliation: Department of Nutrition, Exercise and Sport (NEXS), University of Copenhagen, Copenhagen, Denmark.
authors.forename: N B
authors.initials: NB
authors.lastname: Nordsborg
authors.affiliation: Site de l'Ecole Nationale de Ski et d'Alpinisme, Ecole Nationale des Sports de Montagne, Chamonix, France.
authors.forename: P
authors.initials: P
authors.lastname: Robach
authors.affiliation: Åstrand Laboratory, The Swedish School of Sport and Health Sciences, Stockholm, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Boushel
authors.affiliation: Department of Physical Education, University of Las Palmas de Gran Canaria, Las Palmas, Spain.
authors.forename: J A L
authors.initials: JA
authors.lastname: Calbet
authors.affiliation: Institute of Physiology and Center for Integrative Human Physiology, University of Zürich, Zurich, Switzerland.
authors.forename: C
authors.initials: C
authors.lastname: Lundby
mesh_terms: D000328:Adult; D004921:Erythropoietin / Q000008:administration & dosage*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D006397:Hematinics / Q000008:administration & dosage*; D006706:Homeostasis; D006801:Humans; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D019344:Lactic Acid / Q000097:blood; D007866:Leg / Q000098:blood supply / Q000502:physiology; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply / Q000378:metabolism*; D010101:Oxygen Consumption; D005082:Physical Exertion / Q000502:physiology*; D011188:Potassium / Q000097:blood; D011994:Recombinant Proteins / Q000008:administration & dosage; D012039:Regional Blood Flow; D055815:Young Adult
chemical_list: D006397:Hematinics; D011994:Recombinant Proteins; D004921:Erythropoietin; D019344:Lactic Acid; D011188:Potassium
keywords: K+; Oxygen uptake; lactate; pH