title: Quality assessment and optimization of purified protein samples: why and how?
abstract: Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and misleading observations in downstream applications. In this review, we aim at proposing a simple-to-follow workflow based on an ensemble of widely available physico-chemical technologies, to assess sequentially the essential properties of any protein sample: purity and integrity, homogeneity and activity. Approaches are then suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency.
authors.affiliation: Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France. bertrand.raynal@pasteur.fr.|CNRS-UMR3528, Institut Pasteur, Departement of Structural Biology and Chemistry, Paris, France. bertrand.raynal@pasteur.fr.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Raynal
authors.affiliation: Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France. pascal.lenormand@pasteur.fr.|CNRS-UMR3528, Institut Pasteur, Departement of Structural Biology and Chemistry, Paris, France. pascal.lenormand@pasteur.fr.
authors.forename: Pascal
authors.initials: P
authors.lastname: Lenormand
authors.affiliation: Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France. bruno.baron@pasteur.fr.|CNRS-UMR3528, Institut Pasteur, Departement of Structural Biology and Chemistry, Paris, France. bruno.baron@pasteur.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Baron
authors.affiliation: Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France. sylviane.hoos@pasteur.fr.|CNRS-UMR3528, Institut Pasteur, Departement of Structural Biology and Chemistry, Paris, France. sylviane.hoos@pasteur.fr.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Hoos
authors.affiliation: Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France. patrick.england@pasteur.fr.|CNRS-UMR3528, Institut Pasteur, Departement of Structural Biology and Chemistry, Paris, France. patrick.england@pasteur.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: England
mesh_terms: D011786:Quality Control; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D011994:Recombinant Proteins