title: Differences in serum protein 2D gel electrophoresis patterns of Przewalski's (Mongolian wild horse) and thoroughbred horses.
abstract: The objective of this study was to assess differences in serum protein expression profiles of Przewalski's (Mongolian wild horse) and thoroughbred horses using proteome analysis. The serum proteins were separated by two-dimensional electrophoresis (2-DE) and five different gene products were identified. Proteins represented by the five spots were identified by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry (MS)/MS technology. The identities of all proteins were deduced based on their similarity to proteins in the human plasma protein database. Three proteins (a haptoglobin-2 alpha glycoprotein and two haptoglobin-2beta glycoproteins with different accession numbers) were downregulated in Przewalski's horse sera compared to thoroughbred horse sera. Moreover, two proteins (tetraspanin-18 and pM5) were upregulated in Przewalski's horses compared to thoroughbred horses. Haptoglobin-2 alpha and haptoglobin-2beta may serve as candidate molecules in future studies of inflammation, coagulation, immune modulation and pro-oxidant and antioxidant activity with consequential effects on the entire metabolism of the horse.
authors.affiliation: Genomic Informatics Center, Hankyong National University, Anseong, Republic of Korea; Department of Animal Life and Environment Science, Hankyong National University, Anseong, Republic of Korea.
authors.forename: Enkhbolor
authors.initials: E
authors.lastname: Barsuren
authors.forename: Bandi
authors.initials: B
authors.lastname: Namkhai
authors.forename: Hong Sik
authors.initials: HS
authors.lastname: Kong
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains / Q000097:blood*; D000835:Animals, Wild / Q000097:blood*; D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D015180:Electrophoresis, Gel, Two-Dimensional; D006736:Horses / Q000097:blood*; D006801:Humans; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins
keywords: Przewalski's horse; serum; thoroughbred