title: Novel coiled-coil proteins regulate exocyst association with cortical microtubules in xylem cells via the conserved oligomeric golgi-complex 2 protein.
abstract: Xylem vessel cells develop secondary cell walls in distinct patterns. Cortical microtubules are rearranged into distinct patterns and regulate secondary cell wall deposition; however, it is unclear how exocytotic membrane trafficking is linked to cortical microtubules. Here, we show that the novel coiled-coil proteins vesicle tethering 1 (VETH1) and VETH2 recruit EXO70A1, an exocyst subunit essential for correct patterning of secondary cell wall deposition, to cortical microtubules via the conserved oligomeric Golgi complex (COG) 2 protein. VETH1 and VETH2 encode an uncharacterized domain of an unknown function designated DUF869, and were preferentially up-regulated in xylem cells. VETH1-green fluorescent protein (GFP) and VETH2-GFP co-localized at novel vesicle-like small compartments, which exhibited microtubule plus-end-directed and end-tracking dynamics. VETH1 and VETH2 interacted with COG2, and this interaction promoted the association between cortical microtubules and EXO70A1 These results suggest that the VETH-COG2 complex ensures the correct secondary cell wall deposition pattern by recruiting exocyst components to cortical microtubules.
authors.affiliation: Center for Frontier Research, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411-8540 Japan Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, Shizuoka, 411 8540 Japan Precursory Research for Embryonic Science and Technology Project, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 Japan oda@nig.ac.jp.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Oda
authors.affiliation: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Iida
authors.affiliation: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nagashima
authors.affiliation: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Sugiyama
authors.affiliation: Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002451:Cell Compartmentation; D022162:Cytoplasmic Vesicles / Q000378:metabolism; D011992:Endosomes / Q000378:metabolism; D005089:Exocytosis; D006056:Golgi Apparatus / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana; D052584:Xylem / Q000166:cytology* / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D015415:Biomarkers
keywords: Arabidopsis thaliana; Coiled-coil proteins; Conserved oligomeric Golgi complex; Cortical microtubules; Exocyst; Vesicle trafficking