title: Influenza pH1N1 Virus Accumulated H275Y Mutation in Neuraminidase during Propagation in MDCK Cells.
abstract: Here, we sequenced the genome of the influenza A/Finland/741 M/2014(H1N1) virus and found that the virus accumulated oseltamivir resistance mutation H275Y in its neuraminidase during propagation in cell culture. This indicates that propagation in cell culture modifies virus genomes. The instability of influenza genomes should be taken into consideration during drug-sensitivity studies.
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Polina
authors.initials: P
authors.lastname: Mishel
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Dmitrii
authors.initials: D
authors.lastname: Bychkov
authors.affiliation: Helsinki University Hospital Laboratory, HUSLAB, Helsinki, Finland.
authors.forename: Hannimari
authors.initials: H
authors.lastname: Kallio-Kokko
authors.affiliation: Helsinki University Central Hospital, HUCS, Helsinki, Finland.
authors.forename: Miia
authors.initials: M
authors.lastname: Valkonen
authors.affiliation: Helsinki University Central Hospital, HUCS, Helsinki, Finland.
authors.forename: Anu
authors.initials: A
authors.lastname: Kantele
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Pirkko
authors.initials: P
authors.lastname: Mattila
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Henrikki
authors.initials: H
authors.lastname: Almusa
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Jalovaara
authors.affiliation: The Institute for Molecular Medicine Finland (FIMM), Helsinki, Finland denis.kainov@helsinki.fi.
authors.forename: Denis
authors.initials: D
authors.lastname: Kainov