title: Molecular profiling of Escherichia coli O157:H7 and non-O157 strains isolated from humans and cattle in Alberta, Canada.
abstract: Virulence markers in Shiga toxin-producing Escherichia coli (STEC) and their association with diseases remain largely unknown. This study determines the importance of 44 genetic markers for STEC (O157 and non-O157) from human clinical cases and their correlation to disease outcome. STEC isolated from a cattle surveillance program were also included. The virulence genes tested were present in almost all O157:H7 isolates but highly variable in non-O157 STEC isolates. Patient age was a significant determinant of clinical outcome.
authors.affiliation: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada University of Alberta, Edmonton, Alberta, Canada linda.chui@albertahealthservices.ca.
authors.forename: Linda
authors.initials: L
authors.lastname: Chui
authors.affiliation: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors.forename: Vincent
authors.initials: V
authors.lastname: Li
authors.affiliation: Anses (French Agency for Food, Environmental and Occupational Health and Safety), Food Safety Laboratory, Maisons-Alfort, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Fach
authors.affiliation: Anses (French Agency for Food, Environmental and Occupational Health and Safety), Food Safety Laboratory, Maisons-Alfort, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Delannoy
authors.affiliation: University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Malejczyk
authors.affiliation: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Patterson-Fortin
authors.affiliation: Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
authors.forename: Alan
authors.initials: A
authors.lastname: Poon
authors.affiliation: Alberta Agriculture and Rural Development, Edmonton, Alberta, Canada.
authors.forename: Robin
authors.initials: R
authors.lastname: King
authors.affiliation: Epidemiology and Surveillance Team, Alberta Ministry of Health, Edmonton, Alberta, Canada.
authors.forename: Kimberley
authors.initials: K
authors.lastname: Simmonds
authors.affiliation: Epidemiology and Surveillance Team, Alberta Ministry of Health, Edmonton, Alberta, Canada.
authors.forename: Allison N
authors.initials: AN
authors.lastname: Scott
authors.affiliation: DynaLIFEdx, Edmonton, Alberta, Canada.
authors.forename: Mao-Cheng
authors.initials: MC
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000416:Alberta; D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary*; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016019:Survival Analysis; D037521:Virulence Factors / Q000235:genetics*; D055815:Young Adult
chemical_list: D037521:Virulence Factors