title: Effect of gonadotrophins, oestradiol and insulin on cumulus expansion of Nili Ravi buffalo oocytes.
abstract: The objective of the present study was to investigate the cumulus expansions of Nili Ravi buffalo oocytes during cultured in TCM-199 supplemented with 2 μg/ml oestradiol (E(2)), 0.05 IU/ml recombinant human follicle stimulating hormone (rhFSH), 2IU/ml human chorionic gonadotrophin (hCG), and 0.12 IU/ml insulin (I). The cumulus oocytes complexes (COCs) were collected from 2-8mm follicles from local abattoir ovaries. Supplementation of medium with single hormones showed significant (P<0.0001) increase in mean diameter of COCs with rhFSH except E(2), hCG and insulin after 24 hours compared to the increase in the mean diameter of COCs matured in TCM-199 without any hormonal supplementation. With rhFSH even at 8th hour, significant increase (P<0.001) in cumulus expansion was observed. In combination of hormones the significant (P<0.0001) cumulus expansion was achieved in E(2)+rhFSH treatment group. The non significant (P>0.05) cumulus expansion was observed in treatment groups viz. E(2)+hCG, E(2)+Insulin, rhFSH+hCG, rhFSH+Insulin, hCG+Insulin, E(2)+rhFSH+hCG and E(2)+rhFSH+hCG+Insulin after 24 hours. In conclusion, supplementation of rhFSH alone and in combination with E(2)in TCM-199 has highly significant effect on cumulus expansion.
authors.affiliation: Department of Zoology University of Azad Jammu and Kashmir, Muzaffarabad, Pakistan.
authors.forename: Beenish
authors.initials: B
authors.lastname: Shahid
authors.affiliation: Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Samina
authors.initials: S
authors.lastname: Jalali
authors.affiliation: Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Muhamad Ijaz
authors.initials: MI
authors.lastname: Khan
authors.affiliation: Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Sa
authors.initials: S
authors.lastname: Shami
mesh_terms: D000818:Animals; D002020:Buffaloes; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003470:Culture Media; D054885:Cumulus Cells / Q000187:drug effects* / Q000502:physiology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D043373:Follicle Stimulating Hormone, Human / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
chemical_list: D006063:Chorionic Gonadotropin; D003470:Culture Media; D043373:Follicle Stimulating Hormone, Human; D007328:Insulin; D004958:Estradiol