title: Liposome-Adenoviral hTERT-siRNA Knockdown in Fibroblasts from Keloids Reduce Telomere Length and Fibroblast Growth.
abstract: Keloids, which possess invasive tumor-like behavior, have been clinically challenging to clinicians especially surgeons. Excessive extracellular matrix secreted from fibroblasts is the main histo-pathological feature of keloids. In this study, we transfected hTERT-siRNA into scar fibroblasts by liposome-adenoviral transduction in order to disrupt telomere length homeostasis and influence the cell cycle of fibroblasts. Our results showed that liposome hTERT-siRNA was able to knock down hTERT gene expression in scar fibroblasts. Moreover, the telomerase activity in hTERT-siRNA group was significantly reduced compared with the control groups. And the telomeric length of hTERT-siRNA group was significantly shortened as well. Further, flow cytometry studies and MTT assay demonstrated that apoptosis rate of fibroblasts in liposome hTERT-siRNA group significantly increased. These results indicated that the liposome-mediated hTERT gene transduction could inhibit the growth of fibroblasts in scar tissues suggesting a promising strategy of keloids treatment in the future.
authors.affiliation: Plastic and Cosmetic Center, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Str. Nangang District, Harbin, 150001, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shang
authors.affiliation: Plastic and Cosmetic Center, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Str. Nangang District, Harbin, 150001, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Yu
authors.affiliation: Plastic and Cosmetic Center, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Str. Nangang District, Harbin, 150001, People's Republic of China. drhaolijun@163.com.
authors.forename: Lijun
authors.initials: L
authors.lastname: Hao
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D007627:Keloid / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000172:deficiency / Q000235:genetics*; D059505:Telomere Homeostasis
chemical_list: C509186:TERT protein, human; D019098:Telomerase
keywords: Extracellular matrix; Fibroblast; Keloids; Telomere