title: Direct chemical sequencing of end-labeled RNA.
abstract: Chemical sequencing of RNA relies on the fact that each of the four bases in RNA is susceptible to chemical modification in a different way. In this protocol, end-labeled RNAs are subjected to base-specific chemical modification reactions that make the RNA strand adjacent to the modified base susceptible to cleavage. The chemical modification reaction is base-specific but limited so that not every base in every strand is modified. After cleavage, the resulting set of radioactive fragments is resolved via polyacrylamide gel electrophoresis.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Nilsen
mesh_terms: D000818:Animals; D001483:Base Sequence; D006801:Humans; D012313:RNA
chemical_list: D012313:RNA