title: Crystal structure of a fully glycosylated HIV-1 gp120 core reveals a stabilizing role for the glycan at Asn262.
abstract: The crystal structure of a fully glycosylated HIV-1 gp120 core in complex with CD4 receptor and Fab 17b at 4.5-Å resolution reveals 9 of the 15 N-linked glycans of core gp120 to be partially ordered. The glycan at position Asn262 had the most extensive and well-ordered electron density, and a GlcNAc(2)Man(7) was modeled. The GlcNAc stem of this glycan is largely buried in a cleft in gp120, suggesting a role in gp120 folding and stability. Its arms interact with the stems of neighboring glycans from the oligomannose patch, which is a major target for broadly neutralizing antibodies.
authors.affiliation: Vaccine Research Center, National Institutes of Health, Bethesda, Maryland, 20892; Department of Integrative Structural and Computational Biology, the Scripps Research Institute, La Jolla, California; International AIDS Vaccine Initiative Neutralizing Antibody Center and the Collaboration for AIDS Vaccine Discovery, the Scripps Research Institute, La Jolla, California; Scripps Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery, the Scripps Research Institute, La Jolla, California.
authors.forename: Leopold
authors.initials: L
authors.lastname: Kong
authors.forename: Ian A
authors.initials: IA
authors.lastname: Wilson
authors.forename: Peter D
authors.initials: PD
authors.lastname: Kwong
mesh_terms: D001216:Asparagine / Q000737:chemistry* / Q000378:metabolism*; D001666:Binding Sites, Antibody; D015704:CD4 Antigens / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D006031:Glycosylation; D057809:HEK293 Cells; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000378:metabolism; D015497:HIV-1 / Q000737:chemistry; D006801:Humans; D008358:Mannose / Q000737:chemistry; D008958:Models, Molecular; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism
chemical_list: D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D011134:Polysaccharides; D001216:Asparagine; D008358:Mannose
keywords: HIV-1 gp120; glycan shield; role of N262