title: Human papillomavirus infection in 674 Chinese patients with laryngeal squamous cell carcinoma.
abstract: OBJECTIVE
  Previous reports suggest a strong association between human papillomavirus (HPV) and the etiology of laryngeal squamous cell carcinoma (LSCC). However, clinical data regarding the HPV infection rate among LSCC patients remain largely inconsistent.
  
  
  METHODS
  In total, 674 LSCC patients from three major hospitals in Shanghai were enrolled in this study. We determined the patients' HPV infection status using immunohistochemistry and the GenoArray HPV genotyping assay and calculated their long-term survival rate using the Kaplan-Meier method.
  
  
  RESULTS
  The total P16-positive rate according to immunostaining results was 7.57% (51/674). None of the P16-negative patients were HPV-positive according to the HPV genotyping test. The rate of HPV infection among patients with LSCC was 4.9% (33/674). HPV infection was more common among nonsmokers (P<0.05), nondrinkers (P<0.05), and patients with supraglottic LSCC (P<0.05). Of the 33 HPV-positive patients, 28 (84.8%) were infected with HPV-16, 2 with HPV-18, 1 with HPV-31, 1 with HPV-33 and 1 with HPV-45. The 3-year overall survival rate and progression-free survival rate were higher in HPV-positive than HPV-negative patients, but the difference was not statistically significant (76.3% vs. 70.7%, P = 0.30 and 65.1% vs. 58.3%, P = 0.37, respectively).
  
  
  CONCLUSIONS
  HPV was not a main causal factor in LSCC carcinogenesis in this Chinese population. HPV infection did not alter patients' overall survival or progression-free survival rates in this study.
authors.affiliation: Department of Otolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Department of Head and Neck Surgery, Renji Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Suru
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Yi
authors.affiliation: Department of Head and Neck Surgery, Renji Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Dong
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Children's Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Otolaryngology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; Otolaryngological Institute of Shanghai Jiaotong University, Shanghai, China.
authors.forename: Shankai
authors.initials: S
authors.lastname: Yin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000150:complications* / Q000453:epidemiology; D002681:China / Q000453:epidemiology; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000302:isolation & purification; D058952:Human papillomavirus 31 / Q000302:isolation & purification; D006801:Humans; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000150:complications* / Q000453:epidemiology; D007830:Larynx / Q000473:pathology; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications* / Q000175:diagnosis / Q000453:epidemiology; D055815:Young Adult