title: Differential expression of CYP1A1 and CYP1A2 genes in H4IIE rat hepatoma cells exposed to TCDD and PAHs.
abstract: Rat hepatoma cells H4IIE were treated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polycyclic aromatic hydrocarbons (PAHs) (dibenz(a,h)anthracene, benzo(a)pyrene, benz(a)anthracene, chrysene), low-concentration mixtures of PAHs and TCDD, and environmental mixtures contaminated by PAHs and their derivatives. Expression of the gene battery comprising cytochrome P450 Cyp1a1, Cyp1a2, Cyp1b1, and glutathione-s-transferase Gsta2 and Gstp was investigated using quantitative real time polymerase chain reaction (qRT-PCR) analysis. The results revealed that TCDD induce Cyp1a1>Cyp1a2>Cyp1b1, while PAHs and PAH-containing environmental mixtures induce Cyp1a2>Cyp1a1>Cyp1b1 gene expression pattern. While low-concentration mixtures elicited a more pronounced response in comparison to single treatments, the typical gene expression patterns were not observed. In all samples, Gsta2 was predominantly expressed relative to Gstp. These findings indicate that differential Cyp1a1 and Cyp1a2 expression in the H4IIE cells might be used for detection of PAHs in highly contaminated environmental mixtures, but not in low-concentration mixtures of these compounds.
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia. Electronic address: sonja.kaisarevic@dbe.uns.ac.rs.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kaisarevic
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Vanja
authors.initials: V
authors.lastname: Dakic
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Hrubik
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Glisic
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department of Effect-Directed Analysis, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Urte
authors.initials: U
authors.lastname: Lübcke-von Varel
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Pogrmic-Majkic
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Fa
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Teodorovic
authors.affiliation: Helmholtz Centre for Environmental Research - UFZ, Department of Effect-Directed Analysis, Permoserstr. 15, 04318 Leipzig, Germany.
authors.forename: Werner
authors.initials: W
authors.lastname: Brack
authors.affiliation: University of Novi Sad Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 2, 21000 Novi Sad, Serbia.
authors.forename: Radmila
authors.initials: R
authors.lastname: Kovacevic
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics; D045744:Cell Line, Tumor; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D019388:Cytochrome P-450 CYP1A2; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics; D003580:Cytochromes / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D008113:Liver Neoplasms / Q000235:genetics; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D051381:Rats
chemical_list: D003580:Cytochromes; D007527:Isoenzymes; D000072317:Polychlorinated Dibenzodioxins; D011084:Polycyclic Aromatic Hydrocarbons; C017170:Cyp1a2 protein, rat; C585499:Cyp1b1 protein, rat; D019363:Cytochrome P-450 CYP1A1; D019388:Cytochrome P-450 CYP1A2; D065633:Cytochrome P-450 CYP1B1; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C496558:Gstp1 protein, rat; C119995:glutathione S-transferase alpha
keywords: CYP; Gene expression; H4IIE; Mixtures; PAHs; TCDD