title: High-sensitivity immunoassay with surface plasmon field-enhanced fluorescence spectroscopy using a plastic sensor chip: application to quantitative analysis of total prostate-specific antigen and GalNAcβ1-4GlcNAc-linked prostate-specific antigen for prostate cancer diagnosis.
abstract: A high-sensitivity immunoassay system with surface plasmon field-enhanced fluorescence spectrometry (SPFS) was constructed using a plastic sensor chip and then applied to the detection of total prostate-specific antigen (total PSA) and GalNAcβ1-4GlcNAc-linked prostate-specific antigen (LacdiNAc-PSA) in serum, to discriminate between prostate cancer (PC) and benign prostate hyperplasia (BPH). By using this automated SPFS immunoassay, the detection limit for total PSA in serum was as low as 0.04 pg/mL, and the dynamic range was estimated to be at least five digits. A two-step sandwich SPFS immunoassay for LacdiNAc-PSA was constructed using both the anti-PSA IgG antibody to capture PSA and Wisteria floribunda agglutinin (WFA) for the detection of LacdiNAc. The results of the LacdiNAc-PSA immunoassay with SPFS showed that the assay had a sensitivity of 20.0 pg/mL and permitted the specific distinction between PC and BPH within the PSA gray zone. These results suggested that high-sensitivity automated SPFS immunoassay systems might become a powerful tool for the diagnosis of PC and other diseases.
authors.affiliation: Corporate R&D Headquarters, Konica Minolta, Inc. , No. 1 Sakura-machi, Hino-shi, Tokyo 191-8511, Japan.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Kaya
authors.forename: Tomonori
authors.initials: T
authors.lastname: Kaneko
authors.forename: Shun
authors.initials: S
authors.lastname: Kojima
authors.forename: Yukito
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Youichi
authors.initials: Y
authors.lastname: Ide
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishida
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Suda
authors.forename: Katsuko
authors.initials: K
authors.lastname: Yamashita
mesh_terms: D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D007785:Lactose / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D057230:Limit of Detection; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000097:blood*; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
chemical_list: C093701:N-acetylgalactosaminyl-1-4-N-acetylglucosamine; D017430:Prostate-Specific Antigen; D007785:Lactose