title: MicroRNA-576-3p inhibits proliferation in bladder cancer cells by targeting cyclin D1.
abstract: MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Shiqi
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Xianglai
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Jindan
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Ben
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Urology, the First Affiliated Hospital, Zhejiang University, Hangzhou 310003, Zhejiang Province, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D020413:3' Untranslated Regions; C530104:CCND1 protein, human; C000590114:MIRN576 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1
keywords: bladder cancer; cyclin D1; microRNA-576-3p; proliferation