title: The influence of methylenetetrahydrofolate reductase and thymidylate synthetase gene polymorphisms on lung adenocarcinoma occurrence.
abstract: OBJECTIVE
  Methylenetetrahydrofolate reductase (MTHFR) and thymidylate synthetase (TYMS) are suggested as risk factors for lung cancer. The purpose of this study was to analyze the association of MTHFR C677T polymorphism and variable number tandem repeat 2R/3R and single nucleotide polymorphism G>C in the 3R allele of the TYMS gene with lung adenocarcinoma.
  
  
  METHODS
  A case-control study including lung adenocarcinoma patients and healthy subjects was performed. Restriction fragment length polymorphism analysis was used for genotyping. Descriptive analyses included genotype and allelic frequencies; the odds ratio and 95% confidence interval were calculated as an estimate of relative risk. Significance was set at p<0.05.
  
  
  RESULTS
  A significant difference in CC vs TT+CT MTHFR genotype distribution was observed between patients and controls. There was no significant association between the TYMS polymorphisms and the risk of lung adenocarcinoma.
  
  
  CONCLUSIONS
  The MTHFR 677T allele is likely to have a protective effect against lung adenocarcinoma development.
authors.affiliation: Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Cavic
authors.forename: Ana
authors.initials: A
authors.lastname: Krivokuca
authors.forename: Jelena
authors.initials: J
authors.lastname: Spasic
authors.forename: Ksenija
authors.initials: K
authors.lastname: Brotto
authors.forename: Emina
authors.initials: E
authors.lastname: Malisic
authors.forename: Davorin
authors.initials: D
authors.lastname: Radosavljevic
authors.forename: Sinisa
authors.initials: S
authors.lastname: Radulovic
authors.forename: Radmila
authors.initials: R
authors.lastname: Jankovic
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D016022:Case-Control Studies; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D013940:Thymidylate Synthase / Q000235:genetics*
chemical_list: C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); C000600377:TYMS protein, human; D013940:Thymidylate Synthase