title: Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous blood samples.
abstract: In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Lydie
authors.initials: L
authors.lastname: Canier
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Nimol
authors.initials: N
authors.lastname: Khim
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Saorin
authors.initials: S
authors.lastname: Kim
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Rotha
authors.initials: R
authors.lastname: Eam
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Chanra
authors.initials: C
authors.lastname: Khean
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Kaknika
authors.initials: K
authors.lastname: Loch
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Malen
authors.initials: M
authors.lastname: Ken
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Pieter
authors.initials: P
authors.lastname: Pannus
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Philippe
authors.initials: P
authors.lastname: Bosman
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Jorgen
authors.initials: J
authors.lastname: Stassijns
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Nackers
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: SweetC
authors.initials: S
authors.lastname: Alipon
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Meng Chuor
authors.initials: MC
authors.lastname: Char
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Nguon
authors.initials: N
authors.lastname: Chea
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: William
authors.initials: W
authors.lastname: Etienne
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Martin
authors.initials: M
authors.lastname: De Smet
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Kindermans
authors.affiliation: Institut Pasteur du Cambodge, Phnom Penh, Cambodia; Médecins Sans Frontières, Brussels, Belgium; Epicentre, Paris, France; Médecins Sans Frontières, Tuol Svay Prey I, Chamkarmon, Phnom Penh, Cambodia; National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia dmenard@pasteur-kh.org dmenard@pasteur.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Ménard
mesh_terms: D002160:Cambodia; D016054:DNA, Protozoan; D059788:Dried Blood Spot Testing / Q000379:methods*; D006801:Humans; D008288:Malaria / Q000097:blood* / Q000175:diagnosis* / Q000635:transmission; D018512:Parasitemia; D010961:Plasmodium / Q000145:classification / Q000235:genetics; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012698:Serologic Tests / Q000379:methods*
chemical_list: D016054:DNA, Protozoan