title: Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma.
abstract: Dendritic cells play an important role in determining whether naïve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-β production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.
authors.affiliation: Department of Pediatrics, Pusan National University School of Medicine, Medical Research Institute of Pusan National University Hospital, Pusan 602-739, Korea.
authors.forename: Hye-Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, Pusan National University, Pusan 602-739, Korea.
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Neurosurgery, Konkuk University Hospital, Seoul 380-701, Korea.
authors.forename: Joon
authors.initials: J
authors.lastname: Cho
authors.affiliation: Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: In Duk
authors.initials: ID
authors.lastname: Jung
authors.affiliation: Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Gun Young
authors.initials: GY
authors.lastname: Yoon
authors.affiliation: Department of Physiology, College of Medicine, Korea University, Seoul 136-705, Korea.
authors.forename: Min-Goo
authors.initials: MG
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Sung Jae
authors.initials: SJ
authors.lastname: Shin
authors.affiliation: Department of Physiology, School of Medicine, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
authors.affiliation: Department of Biochemistry, College of Medicine, Konyang University, Daejeon 302-711, Korea.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.affiliation: Cell Signaling and Bioimaging Laboratory, Department of Bio and Brain Engineering, KAIST, Daejeon 305-701, Korea.
authors.forename: Seung-Wook
authors.initials: SW
authors.lastname: Ryu
authors.affiliation: Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University, Chungju 380-701, Korea.
authors.forename: Yeong-Min
authors.initials: YM
authors.lastname: Park
authors.affiliation: National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.
authors.forename: Ji Chang
authors.initials: JC
authors.lastname: You
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D001249:Asthma / Q000628:therapy*; D003713:Dendritic Cells / Q000276:immunology*; D050990:GATA3 Transcription Factor / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000302:isolation & purification*; D006967:Hypersensitivity / Q000628:therapy*; D007167:Immunotherapy; D018836:Inflammation Mediators / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010047:Ovalbumin / Q000008:administration & dosage
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D006360:Heat-Shock Proteins; D018836:Inflammation Mediators; D010047:Ovalbumin