title: Influence of the association between platelet-rich fibrin and bovine bone on bone regeneration. A histomorphometric study in the calvaria of rats.
abstract: This study aimed to investigate the effects of platelet-rich fibrin (PRF) associated or not with Bio-Oss on bone defects in the calvaria of rats. A critical-size defect of 5-mm diameter was performed in the calvaria of 48 rats. These animals were divided into six groups of eight animals each, according to the treatment received: homogeneous clot, autogenous clot, autogenous PRF, homogeneous PRF, Bio-Oss, or Bio-Oss associated with PRF. The animals were euthanized after 30 or 60 days. Bone regeneration was evaluated by histomorphometric analysis. The highest mean percentages of new bone formation at 30 days (54.05% ± 5.78) and 60 days (63.58% ± 5.78) were observed in the Bio-Oss associated with PRF group; in particular, the percentage of new bone at 30 days was significantly higher than that of all of the other groups (P<0.01). At 60 days, the Bio-Oss associated with PRF (63.58% ± 5.78) and Bio-Oss (57.34% ± 5.78) groups had similar results, and both showed a statistical difference compared to the other groups. PRF had a positive effect on bone regeneration only when associated with Bio-Oss.
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil. Electronic address: marinareis89@hotmail.com.
authors.forename: M R
authors.initials: MR
authors.lastname: Oliveira
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: deC Silva
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Ferreira
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Avelino
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil.
authors.forename: I R
authors.initials: IR
authors.lastname: Garcia
authors.affiliation: Federal University of Alfenas, Bairro Centro, Alfenas, MG, Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Mariano
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects*; D002417:Cattle; D005337:Fibrin / Q000502:physiology*; D008297:Male; D008903:Minerals / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012886:Skull / Q000601:surgery*
chemical_list: C077540:Bio-Oss; D008903:Minerals; D005337:Fibrin
keywords: Bone graft; Bone regeneration; Bone transplantation; Fibrin; Platelet-derived growth factor