title: High reproducibility of adhesion formation in rat with meso-stitch approximation of injured cecum and abdominal wall.
abstract: OBJECTIVE
  Peritoneal adhesions following surgery are a common, serious pathology with severe complications. Appropriate animal adhesion models are essential for the assessment of adhesion preventing medical devices. This study introduces a variation of an established rat model in which highest degree adhesions are induced with excellent reproducibility (OPAM=optimized peritoneal adhesion model). Thus, this model seems to be eligible to study effects of adhesion preventing devices.
  
  
  METHODS
  24 Lewis male rats were divided into four groups (OPAM, WSFX, sham-OPAM, sham-WSFX). The OPAM technique comprised cecal abrasion, creation of an abdominal wall defect plus approximation of injured areas by a suture, which was compared to a setting of lesions without suture fixation (WSFX). All rats were sacrificed at day 7. Macroscopic and histopathological evaluations were performed. RESULTS were statistically analyzed using ANOVA and Dunnett's test.
  
  
  RESULTS
  In OPAM rats macroscopic analyses revealed a 90% incidence adhesion of cecum to the abdominal wall, all adhesions imposing as complete agglutination. In WSFX animals incidence of adhesions formation was 75%, while in both sham groups there were no adhesions at all. Histology showed the structure of adhesions with merged smooth muscle of colon and skeletal muscle of abdominal wall in all cases.
  
  
  CONCLUSIONS
  OPAM technique provides adhesions of injured areas with a better probability than with conventional methods. All OPAM adhesions impressed as highest degree adhesions, i.e. agglutination. Due to high reproducibility in incidence and extend of adhesion formation, the OPAM is recommended for testing of adhesion prevention medical devices.
authors.affiliation: 1. Department of General, Visceral and Transplantation Surgery, Hannover Medical School (MHH), Hannover, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Poehnert
authors.affiliation: 2. Department of Pathology, Hannover Medical School (MHH), Hannover, Germany.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Abbas
authors.affiliation: 2. Department of Pathology, Hannover Medical School (MHH), Hannover, Germany.
authors.forename: Hans-Heinrich
authors.initials: HH
authors.lastname: Kreipe
authors.affiliation: 1. Department of General, Visceral and Transplantation Surgery, Hannover Medical School (MHH), Hannover, Germany.
authors.forename: Juergen
authors.initials: J
authors.lastname: Klempnauer
authors.affiliation: 1. Department of General, Visceral and Transplantation Surgery, Hannover Medical School (MHH), Hannover, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Winny
mesh_terms: D034861:Abdominal Wall / Q000473:pathology*; D000818:Animals; D002432:Cecum / Q000293:injuries / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D008297:Male; D010537:Peritoneum / Q000473:pathology; D011183:Postoperative Complications / Q000473:pathology; D011917:Rats, Inbred Lew; D015203:Reproducibility of Results; D000267:Tissue Adhesions / Q000209:etiology* / Q000473:pathology
keywords: Adhesion; Cecal Abrasion; OPAM.; Prevention; Rat