title: LNA probe-based assay for the detection of Tomato black ring virus isolates.
abstract: Tomato black ring virus (TBRV) infects a wide range of economically important plant species worldwide. In the present study we developed a locked nucleic acid (LNA) real-time RT-PCR assay for accurate detection of genetically diverse TBRV isolates collected from different hosts. The assay based on the LNA probe has a wide detection range, high sensitivity, stability and amplification efficiency. The assay amplified all tested TBRV isolates, but no signal was observed for the RNA from other nepoviruses and healthy plant species. Under optimum reaction conditions, the detection limit was estimated around 17 copies of the TBRV target region in total RNA. Real-time RT-PCR with the LNA probe described in this paper will serve as a valuable tool for robust, sensitive and reliable detection of TBRV isolates.
authors.affiliation: Institute of Plant Protection, National Research Institute, Department of Virology and Bacteriology, Wł. Węgorka 20, 60-318 Poznań, Poland. Electronic address: beatahasiow@tlen.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
authors.affiliation: Institute of Plant Protection, National Research Institute, Department of Virology and Bacteriology, Wł. Węgorka 20, 60-318 Poznań, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rymelska
authors.affiliation: Institute of Plant Protection, National Research Institute, Department of Virology and Bacteriology, Wł. Węgorka 20, 60-318 Poznań, Poland.
authors.forename: Natasza
authors.initials: N
authors.lastname: Borodynko
mesh_terms: D018551:Solanum lycopersicum / Q000821:virology*; D017860:Nepovirus / Q000145:classification* / Q000302:isolation & purification*; D015345:Oligonucleotide Probes / Q000235:genetics*; D010935:Plant Diseases / Q000821:virology; D012367:RNA, Viral / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
chemical_list: D015345:Oligonucleotide Probes; D012367:RNA, Viral
keywords: Detection; LNA probe; Real-time RT-PCR; Tomato black ring virus