title: In vivo tumor-targeted dual-modal fluorescence/CT imaging using a nanoprobe co-loaded with an aggregation-induced emission dye and gold nanoparticles.
abstract: As an intensely studied computed tomography (CT) contrast agent, gold nanoparticle has been suggested to be combined with fluorescence imaging modality to offset the low sensitivity of CT. However, the strong quenching of gold nanoparticle on fluorescent dyes requires complicated design and shielding to overcome. Herein, we report a unique nanoprobe (M-NPAPF-Au) co-loading an aggregation-induced emission (AIE) red dye and gold nanoparticles into DSPE-PEG(2000) micelles for dual-modal fluorescence/CT imaging. The nanoprobe was prepared based on a facile method of "one-pot ultrasonic emulsification". Surprisingly, in the micelles system, fluorescence dye (NPAPF) efficiently overcame the strong fluorescence quenching of shielding-free gold nanoparticles and retained the crucial AIE feature. In vivo studies demonstrated the nanoprobe had superior tumor-targeting ability, excellent fluorescence and CT imaging effects. The totality of present studies clearly indicates the significant potential application of M-NPAPF-Au as a dual-modal non-invasive fluorescence/X-ray CT nanoprobe for in vivo tumor-targeted imaging and diagnosis.
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian 271016, PR China; Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Jimei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Chan
authors.initials: C
authors.lastname: Li
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Shuaidong
authors.initials: S
authors.lastname: Huo
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Shubin
authors.initials: S
authors.lastname: Jin
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China. Electronic address: ff_an@aliyun.com.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: An
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian 271016, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Xue
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China.
authors.forename: C I
authors.initials: CI
authors.lastname: Okeke
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian 271016, PR China.
authors.forename: Guiyun
authors.initials: G
authors.lastname: Duan
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian 271016, PR China.
authors.forename: Fengguang
authors.initials: F
authors.lastname: Guo
authors.affiliation: Nano-organic Photoelectronic Laboratory and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Taishan Medical University, Taian 271016, PR China. Electronic address: haojifu@163.com.
authors.forename: Jifu
authors.initials: J
authors.lastname: Hao
authors.affiliation: Laboratory of Molecular Imaging, Department of Radiology, Howard University, Washington, DC 20060, USA.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Wang
authors.affiliation: College of Chemistry and Environmental Science, Chemical Biology Key Laboratory of Hebei Province, Hebei University, Baoding 071002, PR China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Sciences (CAS), Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, No. 11, First North Road, Zhongguancun, Beijing 100190, PR China. Electronic address: liangxj@nanoctr.cn.
authors.forename: Xing-Jie
authors.initials: XJ
authors.lastname: Liang
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D005650:Fumarates / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006046:Gold / Q000493:pharmacokinetics / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000000981:diagnostic imaging*; D013056:Spectrophotometry, Ultraviolet; D014018:Tissue Distribution / Q000187:drug effects; D014057:Tomography, X-Ray Computed
chemical_list: D005456:Fluorescent Dyes; D005650:Fumarates; D006046:Gold; C057444:fumaronitrile
keywords: AIE dye; Dual-modal imaging; Gold nanoparticles; Non-invasive fluorescence imaging; Tumor-targeting; X-ray computed tomography