title: Combined analysis of the tobacco metabolites cotinine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human urine.
abstract: Two of the most widely measured compounds in the urine of people who use tobacco products are cotinine, a major metabolite of the addictive constituent nicotine, and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), a metabolite of the powerful lung carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Thousands of analyses have been reported in the literature, carried out exclusively, to the best of our knowledge, by separate methods. In the study reported here, we have developed a sensitive, accurate, and precise liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring method for the combined analysis of total cotinine (the sum of cotinine and its glucuronide) and total NNAL (the sum of NNAL and its glucuronide). The new method quantifies naturally occurring [(13)C]cotinine to minimize problems associated with the vast differences in concentration of total cotinine and total NNAL in urine. This method should greatly facilitate future determinations of these important compounds.
authors.affiliation: Masonic Cancer Center, University of Minnesota , 2231 6th Street SE, 2-148 CCRB, Minneapolis, Minnesota 55455, United States.
authors.forename: Delshanee
authors.initials: D
authors.lastname: Kotandeniya
authors.forename: Steven G
authors.initials: SG
authors.lastname: Carmella
authors.forename: Xun
authors.initials: X
authors.lastname: Ming
authors.forename: Sharon E
authors.initials: SE
authors.lastname: Murphy
authors.forename: Stephen S
authors.initials: SS
authors.lastname: Hecht
mesh_terms: D002853:Chromatography, Liquid; D003367:Cotinine / Q000378:metabolism / Q000652:urine*; D006801:Humans; D009538:Nicotine / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism / Q000652:urine*; D011725:Pyridines / Q000378:metabolism / Q000652:urine*; D012907:Smoking / Q000378:metabolism / Q000652:urine*; D053719:Tandem Mass Spectrometry; D062789:Tobacco Products
chemical_list: D009602:Nitrosamines; D011725:Pyridines; D009538:Nicotine; C099565:4-(methylnitrosamino)-1-(3-pyridyl)-1-butan-1-ol; D003367:Cotinine