title: Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification.
abstract: To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.
authors.affiliation: a Laboratory of Molecular Genetics and Proteomics, College of Life Science , Capital Normal University , Beijing , China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Han
authors.forename: Xaiobing
authors.initials: X
authors.lastname: Lu
authors.forename: Zitong
authors.initials: Z
authors.lastname: Yu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Wujun
authors.initials: W
authors.lastname: Ma
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D005903:Gliadin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014908:Triticum / Q000737:chemistry* / Q000145:classification / Q000378:metabolism
chemical_list: D005903:Gliadin
keywords: capillary electrophoresis; gliadin; reversed-phase ultra performance liquid chromatography; seed; wheat