title: A novel function for fibroblast growth factor 21: stimulation of NADPH oxidase-dependent ROS generation.
abstract: Fibroblast growth factor 21 (FGF-21) is a major paracrine and endocrine regulator of metabolic homeostasis. Here we demonstrate that FGF-21 is also a potent mediator of innate immunity. Double-staining flow cytometry identified neutrophils and monocytes as the main sources of FGF-21 among circulating leukocytes. Functional assays showed that FGF-21 stimulates phagocytosis and production of reactive oxygen species in neutrophil-like HL-60 cells and monocytic THP-1 cells. The mechanism of action of FGF-21 was observed to involve FGF receptor activation, signal transduction through the PI3K/Akt pathway, and stimulation of NADPH oxidase activity. This study indicates that FGF-21 could be an attractive target for the management of inflammatory disorders.
authors.affiliation: College of Life Science, Northeast Agricultural University, Harbin, 150030, People's Republic of China, wangwenfei@neau.edu.cn.
authors.forename: Wen-fei
authors.initials: WF
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Ming-yao
authors.initials: MY
authors.lastname: Liu
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Zhao
authors.forename: Tong
authors.initials: T
authors.lastname: Zhang
authors.forename: Yong-bi
authors.initials: YB
authors.lastname: Yang
authors.forename: Hong-xue
authors.initials: HX
authors.lastname: Cao
authors.forename: Xiao-hui
authors.initials: XH
authors.lastname: Han
authors.forename: De-shan
authors.initials: DS
authors.lastname: Li
mesh_terms: D000328:Adult; D000818:Animals; D005260:Female; D005346:Fibroblast Growth Factors / Q000502:physiology*; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology*; D008297:Male; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D017468:Receptors, Fibroblast Growth Factor; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D019255:NADPH Oxidases