title: Transcriptome-wide analysis of SAMe superfamily to novelty phosphoethanolamine N-methyltransferase copy in Lonicera japonica.
abstract: The S-adenosyl-L-methionine-dependent methyltransferase superfamily plays important roles in plant development. The buds of Lonicera japonica are used as Chinese medical material and foods; chinese people began domesticating L. japonica thousands of years ago. Compared to the wild species, L. japonica var. chinensis, L. japonica gives a higher yield of buds, a fact closely related to positive selection over the long cultivation period of the species. Genome duplications, which are always detected in the domestic species, are the source of the multifaceted roles of the functional gene. In this paper, we investigated the evolution of the SAMe genes in L. japonica and L. japonica var. chinensis and further analyzed the roles of the duplicated genes among special groups. The SAMe protein sequences were subdivided into three clusters and several subgroups. The difference in transcriptional levels of the duplicated genes showed that seven SAMe genes could be related to the differences between the wild and the domesticated varieties. The sequence diversity of seven SAMe genes was also analyzed, and the results showed that different gene expression levels between the varieties could not be related to amino acid variation. The transcriptional level of duplicated PEAMT could be regulated through the SAM-SAH cycle.
authors.affiliation: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China. yuanyuan@icmm.ac.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China. qlj0810139@163.com.
authors.forename: Linjie
authors.initials: L
authors.lastname: Qi
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing 100029, China. junyu@big.ac.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yu
authors.affiliation: CAS Key Laboratory of Genome Sciences and Information, Beijing 100029, China. wangxm@big.ac.cn.
authors.forename: Xumin
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China. huangluqi01@126.com.
authors.forename: Luqi
authors.initials: L
authors.lastname: Huang
mesh_terms: D020440:Gene Duplication; D020869:Gene Expression Profiling; D031239:Lonicera / Q000201:enzymology* / Q000235:genetics*; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D059467:Transcriptome
chemical_list: D008780:Methyltransferases; C102844:phosphoethanolamine methyltransferase