title: Programmable engineering of a biosensing interface with tetrahedral DNA nanostructures for ultrasensitive DNA detection.
abstract: Self-assembled DNA nanostructures with precise sizes allow a programmable "soft lithography" approach to engineer the interface of electrochemical DNA sensors. By using millimeter-sized gold electrodes modified with several types of tetrahedral DNA nanostructures (TDNs) of different sizes, both the kinetics and thermodynamics of DNA hybridization were profoundly affected. Because each DNA probe is anchored on an individual TDN, its lateral spacing and interactions are finely tuned by the TDN size. By simply varying the size of the TDNs, the hybridization time was decreased and the hybridization efficiency was increased. More significantly, the detection limit for DNA detection was tuned over four orders of magnitude with differentially nanostructured electrodes, and achieved attomolar sensitivity with polymeric enzyme amplification.
authors.affiliation: Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800 (China).
authors.forename: Meihua
authors.initials: M
authors.lastname: Lin
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.forename: Guobao
authors.initials: G
authors.lastname: Zhou
authors.forename: Jianbang
authors.initials: J
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lu
authors.forename: Jimin
authors.initials: J
authors.lastname: Gao
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.forename: Jiye
authors.initials: J
authors.lastname: Shi
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Zuo
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004867:Equipment Design; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D013499:Surface Properties
chemical_list: D004247:DNA
keywords: DNA nanostructures; biosensors; electrochemistry; soft lithography; surface chemistry