title: mir-155 regulates cardiac allograft rejection by targing the expression of suppressor of cytokine signaling-1 (DOCS1) in dendritic cells.
abstract: Previously, we observed that mir-155 is induced during dendritic cell (DC) differentiation. We now demon-strated convincing evidence indicating that mir-155 promotes DC maturation and regulates its capacity for antigen presentation and induction of alloreactive T cell activation. Interestingly, the induction of miR-155 expression in DCs is dependent on the TLR4/Myd88/NF-κB signaling. Our mechanistic studies further revealed that SOCS1 is a direct target for mir-155, and by binding to its 3'UTR, mir-155 is likely to affect SOCS1 translation. Suppression of mir-155 expression in DCs significantly attenuated LPS-induced DC maturation along with reduced capability to stimulate allogeneic T cell proliferation. As a result, administration of antagomiR-155 provided protection for cardiac allografts from rejection. Together, our data support that suppression of miR-155 in DCs could be a viable therapeutic strategy for prevention and treatment of allograft rejection in clinical setting of transplantation.
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Guo
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University Shanghai, China.
authors.forename: Mingjian
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University Shanghai, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Hu
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University Shanghai, China.
authors.forename: Chaozhou
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University Shanghai, China.
authors.forename: Yongzhao
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Urology and Organ Transplantation Institute of PLA, Changzheng Hospital, Second Military Medical University Shanghai, China.
authors.forename: Yushan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Medical Immunology, Second Military Medical University Shanghai, China.
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Wang
keywords: allograft; dendritic cells; immune response; miR-155