title: Actin-myosin network is required for proper assembly of influenza virus particles.
abstract: Actin filaments are known to play a central role in cellular dynamics. After polymerization of actin, various actin-crosslinking proteins including non-muscle myosin II facilitate the formation of spatially organized actin filament networks. The actin-myosin network is highly expanded beneath plasma membrane. The genome of influenza virus (vRNA) replicates in the cell nucleus. Then, newly synthesized vRNAs are nuclear-exported to the cytoplasm as ribonucleoprotein complexes (vRNPs), followed by transport to the beneath plasma membrane where virus particles assemble. Here, we found that, by inhibiting actin-myosin network formation, the virus titer tends to be reduced and HA viral spike protein is aggregated on the plasma membrane. These results indicate that the actin-myosin network plays an important role in the virus formation.
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Kumakura
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan. Electronic address: ats-kawaguchi@md.tsukuba.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawaguchi
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, Tsukuba 305-8575, Japan. Electronic address: knagata@md.tsukuba.ac.jp.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000502:physiology*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000502:physiology*; D007251:Influenza, Human / Q000378:metabolism* / Q000821:virology; D009218:Myosins / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D000199:Actins; D009218:Myosins
keywords: Blebbistatin; Influenza virus assembly; Non-muscle myosin II