title: α-Linolenic acid supplementation in BioXcell® extender can improve the quality of post-cooling and frozen-thawed bovine sperm.
abstract: The present study was conducted to determine the effects of supplementing α-linolenic acid (ALA) into BioXcell(®) extender on post-cooling, post-thawed bovine spermatozoa and post thawed fatty acid composition. Twenty-four semen samples were collected from three bulls using an electro-ejaculator. Fresh semen samples were evaluated for general motility using computer assisted semen analyzer (CASA) whereas morphology and viability with eosin-nigrosin stain. Semen samples extended into BioXcell(®) were divided into five groups to which 0, 3, 5, 10 and 15 ng/ml of ALA were added, respectively. The treated samples were incubated at 37°C for 15 min for ALA uptake by sperm cells before being cooled for 2 h at 5°C. After evaluation, the cooled samples were packed into 0.25 ml straws and frozen in liquid nitrogen for 24 h before thawing and evaluation for semen quality. Evaluation of cooled and frozen-thawed semen showed that the percentages of all the sperm parameters improved with 5 ng/ml ALA supplement. ALA was higher in all treated groups than control groups than control group. In conclusion, 5 ng/ml ALA supplemented into BioXcell(®) extender improved the cooled and frozen-thawed quality of bull spermatozoa.
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Sindh Agriculture University, Tando Jam, Pakistan.
authors.forename: Asmatullah
authors.initials: A
authors.lastname: Kaka
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Haron
authors.initials: H
authors.lastname: Wahid
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia. Electronic address: rosninanuris@upm.edu.my.
authors.forename: Yusoff
authors.initials: Y
authors.lastname: Rosnina
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Nurhusien
authors.initials: N
authors.lastname: Yimer
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: A M
authors.initials: AM
authors.lastname: Khumran
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Kazhal
authors.initials: K
authors.lastname: Sarsaifi
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Sindh Agriculture University, Tando Jam, Pakistan.
authors.forename: Atique Ahmed
authors.initials: AA
authors.lastname: Behan
authors.affiliation: Department of Veterinary Clinical Studies, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Sindh Agriculture University, Tando Jam, Pakistan.
authors.forename: Ubedullah
authors.initials: U
authors.lastname: Kaka
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: M
authors.initials: M
authors.lastname: Ebrahimi
mesh_terms: D000177:Acrosome / Q000187:drug effects; D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D005615:Freezing; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000494:pharmacology*
chemical_list: D003451:Cryoprotective Agents; D005227:Fatty Acids; D017962:alpha-Linolenic Acid
keywords: ALA; BioXcell(®); Bull semen; Cryopreservation