title: Inhibitory effects of Cyperus digitatus extract on human platelet function in vitro.
abstract: The purpose of this research was to investigate the mechanisms of antiplatelet action of Cyperus digitatus. The antiplatelet action of C. digitatus was studied on platelet function: secretion, adhesion, aggregation, and sCD40L release. The platelet ATP secretion and aggregation were significantly inhibited by CDA (ethyl acetate extract) at 0.1 mg/ml and after the incubation of whole blood with CDA, the platelet coverage was inhibited by 96 ± 3% (p < 0.001). At the same concentration, CDA significantly decreased sCD40L levels. The mechanism of antiplatelet action of CDA could be by NF-κB inhibition and that is cAMP independent. In conclusion, C. digitatus extract may serve as a new source of antiplatelet agents for food and nutraceutical applications.
authors.affiliation: a Department of Clinical Biochemistry and Immunohematology , Faculty of Health Sciences. Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca , Talca , Chile .|b Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional , Gore Maule, R09I2001 , Chile , and.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Fuentes
authors.affiliation: c Chemical Institute of Natural Resources, Universidad de Talca , Talca , Chile.
authors.forename: Oscar
authors.initials: O
authors.lastname: Forero-Doria
authors.affiliation: a Department of Clinical Biochemistry and Immunohematology , Faculty of Health Sciences. Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca , Talca , Chile .|b Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional , Gore Maule, R09I2001 , Chile , and.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Alarcón
authors.affiliation: a Department of Clinical Biochemistry and Immunohematology , Faculty of Health Sciences. Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), Universidad de Talca , Talca , Chile .|b Centro de Estudios en Alimentos Procesados (CEAP), CONICYT-Regional , Gore Maule, R09I2001 , Chile , and.
authors.forename: Iván
authors.initials: I
authors.lastname: Palomo
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism*; D023201:CD40 Ligand / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D029786:Cyperus / Q000737:chemistry*; D006801:Humans; D010936:Plant Extracts / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D010979:Platelet Function Tests
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D023201:CD40 Ligand; D000255:Adenosine Triphosphate; D000242:Cyclic AMP
keywords: Antiplatelet activity; NF-κB pathway; cardiovascular diseases; cyperus digitatus; sCD40L