title: A novel ultra performance liquid chromatography-tandem mass spectrometry method for the determination of sucrose octasulfate in dog plasma.
abstract: A novel, specific and sensitive bioanalytical method has been developed for the determination of sucrose octasulfate (SOS) in dog plasma and urine using ion-pair reversed-phase ultraperformance liquid chromatography coupled with electrospray triple quadruple mass spectrometry (IPRP-UPLC ESI MS/MS). (13)C-labeled sucrose octasulfate-(13)C12 sodium salt is used as the internal standard. 200 μL of plasma or serum sample is extracted using weak anion exchange solid phase cartridge. In this method, a polar amide column is employed for the liquid chromatograph (LC) separation while the diethylamine and formic acid buffer is used as the ion-pairing reagent. The low limitation of quantitation of sucrose octasulfate is 0.20 ng on the column with a signal to noise ratio larger than 50. Parameters such as linearity, accuracy and precision have been validated in full compliance with the FDA guidelines for the bioanalytical method development and validation. A linear regression model fit the calibration curve very well with R>0.99. The bias and coefficient of variation of all levels of QCs are within the range of 15%. The selectivity, matrix effect and stabilities of analytes in solution and matrix have also been evaluated and the results met the acceptance criteria according to the guidelines. Based on these results, the method has qualified to analyze sucrose octasulfate in dog plasma for clinic research. This method has been applied to 1000 preclinical samples.
authors.affiliation: Diteba Research Laboratory Inc., 1620 Tech Avenue, Unit 3, Mississauga, ON L4W 5P4, Canada. Electronic address: yke2005@gmail.com.
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Diteba Research Laboratory Inc., 1620 Tech Avenue, Unit 3, Mississauga, ON L4W 5P4, Canada. Electronic address: steveli36@yahoo.ca.
authors.forename: Steve Lianghong
authors.initials: SL
authors.lastname: Li
authors.affiliation: Diteba Research Laboratory Inc., 1620 Tech Avenue, Unit 3, Mississauga, ON L4W 5P4, Canada.
authors.forename: Linda Dongxia
authors.initials: LD
authors.lastname: Chang
authors.affiliation: Diteba Research Laboratory Inc., 1620 Tech Avenue, Unit 3, Mississauga, ON L4W 5P4, Canada.
authors.forename: Theo
authors.initials: T
authors.lastname: Kapanadze
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004355:Drug Stability; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013395:Sucrose; C053081:sucrose octasulfate
keywords: Ion pairing; Sucrose octasulfate; UPLC–MS/MS