title: YnfA , a SMR family efflux pump is abundant in Escherichia coli isolates from urinary infection.
abstract: A quantitative study was undertaken to determine the expression level of different efflux pumps in multi-drug-resistant (MDR) Escherichia coli isolates from urinary infection. We have determined the presence of different efflux pumps and measured the expression level of tolC, mdfA, norE and ynfA genes among 48 isolates by quantitative real-time PCR. The expression level of tolC and ynfA was constantly high and observed among 75-80% of isolates, whereas mdfA and norE were expressed occasionally. Our findings suggest that ynfA, a new SMR efflux pump gene family member increases the antibiotics' resistance in E. coli.
authors.forename: S K
authors.initials: SK
authors.lastname: Sarkar
authors.forename: A
authors.initials: A
authors.lastname: Bhattacharyya
authors.affiliation: Central Research Facility, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
authors.forename: S M
authors.initials: SM
authors.lastname: Mandal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000382:microbiology*; D020869:Gene Expression Profiling; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction; D014552:Urinary Tract Infections / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins