title: Mechanism for the antibacterial action of epigallocatechin gallate (EGCg) on Bacillus subtilis.
abstract: Catechins are a class of polyphenols and have high anti-bacterial activity against various microorganisms. Here, we report the mechanism for antibacterial activity of epigallocatechin gallate (EGCg) against Gram-positive bacteria Bacillus subtilis, which is highly sensitive to EGCg. Transmission electron microscope analysis revealed that deposits containing EGCg were found throughout the cell envelope from the outermost surface to the outer surface of cytoplasmic membrane. Aggregating forms of proteins and EGCg were identified as spots that disappeared or showed markedly decreased intensity after the treatment with EGCg compared to the control by two-dimensional electrophoresis. Among the identified proteins included 4 cell surface proteins, such as oligopeptide ABC transporter binding lipoprotein, glucose phosphotransferase system transporter protein, phosphate ABC transporter substrate-binding protein, and penicillin-binding protein 5. Observations of glucose uptake of cells and cell shape B. subtilis after the treatment with EGCg suggested that EGCg inhibits the major functions of these proteins, leading to growth inhibition of B. subtilis.
authors.affiliation: a Kao Corporation, Global R&D-Safety Science , Tochigi , Japan.
authors.forename: Motokazu
authors.initials: M
authors.lastname: Nakayama
authors.forename: Kanami
authors.initials: K
authors.lastname: Shimatani
authors.forename: Tadahiro
authors.initials: T
authors.lastname: Ozawa
authors.forename: Naofumi
authors.initials: N
authors.lastname: Shigemune
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tomiyama
authors.forename: Koji
authors.initials: K
authors.lastname: Yui
authors.forename: Mao
authors.initials: M
authors.lastname: Katsuki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ikeda
authors.forename: Ai
authors.initials: A
authors.lastname: Nonaka
authors.forename: Takahisa
authors.initials: T
authors.lastname: Miyamoto
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D002392:Catechin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005947:Glucose / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry / Q000378:metabolism; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D010100:Oxygen / Q000378:metabolism; D013171:Spores, Bacterial / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D008074:Lipoproteins; C046506:oligopeptide-binding protein, bacteria; D002392:Catechin; C045651:epigallocatechin gallate; D005947:Glucose; D010100:Oxygen
keywords: Bacillus subtilis; catechins; epigallocatechin gallate