title: The dynamics of heat shock system activation in Monomac-6 cells upon Helicobacter pylori infection.
abstract: Immune system cells, particularly phagocytes, are exposed to direct contact with pathogens. Because of its nature - elimination of pathogenes - their cytoprotective systems supposed to be quick and forceful. Physiological consequence of phagocytosis for the phagocyte is the apoptotic death to prevent the eventual survival of bacteria as intracellular parasites. However, in some cases, defense systems used by the bacteria force the immune cells to prolong the contact with the pathogen for its effective elimination. Experiments were performed on Monomac-6 cells exposed to live CagA, VacA expressing Helicobacter pylori (H. pylori) over different period of time. Total cellular RNA, cytoplasmic and nuclear proteins were isolated for polymerase chain reaction, Western-blot and electrophoretic mobility shift assay, respectively. We found that Monomac-6 cells infection with H. pylori resulted in the translocation of the entire cellular content of the heat shock protein 70 (HSP70) into the cytoplasm, where its presence could protect cell against toxic products of engulfed bacteria and premature apoptosis. At the same time the nuclear translocation of heat shock factor 1 (HSF-1) and activation of HSP70 gene transcription was noticed. Action of HSP70 might to postpone monocyte apoptosis through protecting cytoplasmic and nuclear proteins from damaging effect of bacterial products, what could be the defending mechanism against the toxic stress caused by engulfed bacteria and provide the immune cell with the sufficient amount of time required for neutralization of the bacteria from phagosomes, even at the expense of temporary lack of the protection of nuclear proteins.
authors.affiliation: Faculty of Health Sciences, Department of Medical Physiology, Jagiellonian University Medical College, Cracow, Poland. piotr.pierzchalski@uj.edu.pl.
authors.forename: P
authors.initials: P
authors.lastname: Pierzchalski
authors.forename: M
authors.initials: M
authors.lastname: Jastrzebska
authors.forename: P
authors.initials: P
authors.lastname: Link-Lenczowski
authors.forename: A
authors.initials: A
authors.lastname: Leja-Szpak
authors.forename: J
authors.initials: J
authors.lastname: Bonior
authors.forename: J
authors.initials: J
authors.lastname: Jaworek
authors.forename: K
authors.initials: K
authors.lastname: Okon
authors.forename: P
authors.initials: P
authors.lastname: Wojcik
mesh_terms: D000942:Antigens, Bacterial / Q000378:metabolism; D017209:Apoptosis; D001426:Bacterial Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D053938:DNA Fragmentation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D018869:Heat-Shock Response; D016481:Helicobacter Infections / Q000378:metabolism*; D016480:Helicobacter pylori / Q000378:metabolism; D006801:Humans; D009000:Monocytes / Q000378:metabolism*; D011487:Protein Conformation; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D014157:Transcription Factors; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori