title: Co-fermentation of hemicellulose and starch from barley straw and grain for efficient pentoses utilization in acetone-butanol-ethanol production.
abstract: This study aims to efficiently use hemicellulose-based biomass for ABE (acetone-butanol-ethanol) production by co-fermentation with starch-based biomass. Two processes were investigated: (I) co-fermentation of sugars derived from hemicellulose and starch in a mixture of barley straw and grain that was pretreated with dilute acid; (II) co-fermentation of straw hemicellulosic hydrolysate and gelatinized grain slurry in which the straw was pretreated with dilute acid. The two processes produced 11.3 and 13.5 g/L ABE that contains 7.4 and 7.8 g/L butanol, respectively. In process I, pretreatment with 1.0% H2SO4 resulted in better ABE fermentability than with 1.5% H2SO4, but only 19% of pentoses were consumed. In process II, 95% of pentoses were utilized even in the hemicellulosic hydrolysate pretreated with more severe condition (1.5% H2SO4). The results suggest that process II is more favorable for hemicellulosic biomass utilization, and it is also attractive for sustainable biofuel production due to great biomass availability.
authors.affiliation: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland. Electronic address: ming.yang@uef.fi.
authors.forename: Ming
authors.initials: M
authors.lastname: Yang
authors.affiliation: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors.forename: Suvi
authors.initials: S
authors.lastname: Kuittinen
authors.affiliation: College of Forestry, Northwest A&F University, 3 Taicheng Road, 712100 Yangling, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, University of Eastern Finland, P.O. Box 1627, FI70211 Kuopio, Finland.
authors.forename: Jouko
authors.initials: J
authors.lastname: Vepsäläinen
authors.affiliation: Department of Biology, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Keinänen
authors.affiliation: School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland.
authors.forename: Ari
authors.initials: A
authors.lastname: Pappinen
mesh_terms: D000096:Acetone / Q000378:metabolism; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005780:Gelatin; D001467:Hordeum / Q000378:metabolism*; D006868:Hydrolysis; D010429:Pentoses / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*
chemical_list: D000440:Butanols; D003470:Culture Media; D010429:Pentoses; D011134:Polysaccharides; D000096:Acetone; D000431:Ethanol; C007916:hemicellulose; D005780:Gelatin; D013213:Starch
keywords: ABE (acetone–butanol–ethanol); Co-fermentation; Hemicellulose; Pentoses; Starch