title: Interneuron activity leads to initiation of low-voltage fast-onset seizures.
abstract: Seizures in temporal lobe epilepsy can be classified as hypersynchronous and low-voltage fast according to their onset patterns. Experimental evidence suggests that low-voltage fast-onset seizures mainly result from the synchronous activity of γ-aminobutyric acid-releasing cells. In this study, we tested this hypothesis using the optogenetic control of parvalbumin-positive interneurons in the entorhinal cortex, in the in vitro 4-aminopyridine model. We found that both spontaneous and optogenetically induced seizures had similar low-voltage fast-onset patterns. In addition, both types of seizures presented with higher ripple than fast ripple rates. Our data demonstrate the involvement of interneuronal networks in the initiation of low-voltage fast-onset seizures.
authors.affiliation: Montreal Neurological Institute and Departments of Neurology and Neurosurgery, McGill University Montreal, Quebec, Canada.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Shiri
authors.forename: Frédéric
authors.initials: F
authors.lastname: Manseau
authors.forename: Maxime
authors.initials: M
authors.lastname: Lévesque
authors.forename: Sylvain
authors.initials: S
authors.lastname: Williams
authors.forename: Massimo
authors.initials: M
authors.lastname: Avoli
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000818:Animals; D055724:Electrophysiological Phenomena / Q000502:physiology*; D018728:Entorhinal Cortex / Q000166:cytology / Q000503:physiopathology*; D059330:GABAergic Neurons / Q000166:cytology / Q000502:physiology*; D007395:Interneurons / Q000166:cytology / Q000502:physiology*; D051379:Mice; D009415:Nerve Net / Q000166:cytology / Q000503:physiopathology; D062308:Optogenetics; D010320:Parvalbumins / Q000378:metabolism; D018408:Patch-Clamp Techniques; D012640:Seizures / Q000503:physiopathology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D010320:Parvalbumins; D005680:gamma-Aminobutyric Acid; D015761:4-Aminopyridine