title: Effect of the accuracy of pH control on hydrogen fermentation.
abstract: pH, known as the most important parameter in H2 fermentation, cannot be precisely controlled in a scaled-up fermenter as in a lab fermenter. In the preset work, to assess the effect of pH control accuracy on H2 fermentation, the pH was controlled at 6.0±0.1, 6.0±0.3, 6.0±0.5, 6.0±0.7, and 6.0±0.9 during batch fermentation of food waste. Up to deviation of ±0.3, a high H2 yield of 1.67-1.73 mol H2/mol hexose(added) was attained with producing butyrate as a major metabolite (>70% of total organic acids produced). A huge drop of H2 production, however, was observed at deviation >±0.5 with lowered substrate utilization and increased production of lactate. Next generation sequencing results showed that Clostridium was found to be the dominant genus (76.4% of total number of sequences) at deviation of ±0.1, whereas the dominant genus was changed to lactic acid bacteria such as Streptococcus and Lactobacillus with increase of deviation value.
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Chungman
authors.initials: C
authors.lastname: Moon
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Division of Renewable Energy Engineering, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea.
authors.forename: Sujin
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Civil and Environmental Engineering, KAIST, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Yeo-Myeong
authors.initials: YM
authors.lastname: Yun
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Mo-Kwon
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea.
authors.forename: Dong-Hoon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: New Technology Research Team, Korea District Heating Corp. R&D Institute, 781 Yangjae-daero, Gangnam-gu, Seoul 135-220, Republic of Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Kang
authors.affiliation: New Technology Research Team, Korea District Heating Corp. R&D Institute, 781 Yangjae-daero, Gangnam-gu, Seoul 135-220, Republic of Korea.
authors.forename: Seung-Shin
authors.initials: SS
authors.lastname: Kwak
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, 152 Gajeong-ro, Yuseong-gu, Daejeon 305-343, Republic of Korea; Division of Renewable Energy Engineering, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 305-350, Republic of Korea. Electronic address: bmmskim@kier.re.kr.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kim
mesh_terms: D001419:Bacteria / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D005285:Fermentation; D005502:Food; D015723:Gene Library; D059014:High-Throughput Nucleotide Sequencing; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013045:Species Specificity; D014866:Waste Products / Q000032:analysis
chemical_list: D002264:Carboxylic Acids; D014866:Waste Products; D006859:Hydrogen
keywords: Butyrate; Hydrogen fermentation; Lactate; Next generation sequencing; pH deviation