title: Silencing of PINK1 inhibits insulin-like growth factor-1-mediated receptor activation and neuronal survival.
abstract: The etiology of Parkinson's disease remains unknown. Mutations in PINK1 have provided an understanding of the molecular mechanisms of this pathology. PINK1 and Parkin are important in the dismissal of dysfunctional mitochondria. However, the role of PINK1 in the control of neuronal survival pathways is not clear. To determine the role of PINK1 in the control of the phosphatidyl inositol 3-kinase (PI3K)/Akt pathway mediated by insulin-like grow factor type 1 (IGF-1), we use a model of mesencephalic neurons (CAD cells), which were transfected with lentiviral PINK1 shRNA or control shRNA constructs. Silencing of PINK1 was determined by RT-PCR and immunoblotting; cell viability was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays; proteins of the PI3K/Akt signaling pathway were tested by immunoblotting and IGF-1 receptor, and mitochondria were examined using fluorescence microscopy. PINK1 shRNA-transfected cells showed a reduction in cell survival compared to control shRNA cells. Exposure to IGF-1 induced a rapid and high increase in the phosphorylation level of IGF-1 receptor in control shRNA-transfected cells; however, silencing of PINK1 decreases phosphorylation level of IGF-1 receptor and downstream target proteins such as Akt, GSK3-beta, IRS-1, and hexokinase. Our results further suggest that PINK1 may be regulating the PI3K/Akt neuronal survival pathway through tyrosine kinase receptors such as IGF-1 receptor.
authors.affiliation: Neuroscience and Cell Death Group, Faculty of Medicine and Institute of Genetics, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: María J
authors.initials: MJ
authors.lastname: Contreras-Zárate
authors.forename: Andrea
authors.initials: A
authors.lastname: Niño
authors.forename: Liliana
authors.initials: L
authors.lastname: Rojas
authors.forename: Humberto
authors.initials: H
authors.lastname: Arboleda
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Arboleda
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D020868:Gene Silencing; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D038362:Glycogen Synthase Kinase 3; D000071679:Glycogen Synthase Kinase 3 beta; D006593:Hexokinase; D055504:Insulin Receptor Substrate Proteins; D007334:Insulin-Like Growth Factor I; D019869:Phosphatidylinositol 3-Kinases; D011494:Protein Kinases; D051057:Proto-Oncogene Proteins c-akt; D000099202:PTEN-Induced Putative Kinase; C526292:IRS1 protein, human