title: RNA-seq based on transcriptome reveals differ genetic expressing in Chlamys farreri exposed to carcinogen PAHs.
abstract: The effects of a mixture of carcinogen polycyclic aromatic hydrocarbons (BaP, BaA, BbF and CHR) on transcriptional responses in the digestive gland of scallop, Chlamys farreri, were investigated by high-throughput RNA-sequencing (RNA-seq) technologies. In total, 209 and 260 genes were identified as significantly up- or down-regulated, respectively. Functional analysis based on gene ontology (GO) classification system and the Kyoto encyclopedia of genes and genomes (KEGG) database revealed that PAHs significantly altered the expression of genes involved in stress response, detoxication, antioxidation which were extensively discussed. In particular, CYP450 2P1 and QO mRNA expression were found to be up-regulated by exposure to PAHs mixture, suggesting that CYP450 2P1 and QO mRNA expression can be a potential marker for prediction of the biological effects of a mixture of PAHs on scallops.
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Fengxiao
authors.initials: F
authors.lastname: Hu
mesh_terms: D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics; D020869:Gene Expression Profiling; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D049897:Pectinidae / Q000187:drug effects* / Q000235:genetics; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D002273:Carcinogens; D018840:HSP70 Heat-Shock Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System
keywords: Carcinogen PAHs; Chlamys farreri; Differential expression; Digital gene expression