title: Garcinielliptone FC: antiparasitic activity without cytotoxicity to mammalian cells.
abstract: Garcinielliptone FC (GFC) is a natural prenylated benzophenone found in the seeds of Platonia insignis Mart. (Clusiaceae), a native Brazilian plant. It has been chemically characterized and it is known that GFC has several biological activities such as antioxidant and vasorelaxant properties. In this study, we report the in vitro effect of GFC against the blood fluke Schistosoma mansoni, the parasite responsible for schistosomiasis mansoni. The anti-S. mansoni activity and cytotoxicity toward mammalian cells were determined for the compound. GFC⩾6.25 μM showed antischistosomal activity and confocal laser scanning microscopy analysis demonstrated several morphological alterations on the tegument of worms, and a correlation between viability and tegumental damage was observed. In addition, at sub-lethal concentrations of GFC (⩽3.125 μM), the number of S. mansoni eggs was reduced. More importantly, GFC exhibited no activity toward mammalian cells and, therefore, there is an appreciable selectivity of this compound against the helminths. In conclusion, these findings indicate the potential of GFC as an antiparasitic agent.
authors.affiliation: Programa de Pós-graduação em Biotecnologia, Rede Nordeste de Biotecnologia, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Ana P
authors.initials: AP
authors.lastname: Silva
authors.affiliation: Núcleo de Pesquisa em Doenças Negligenciadas, Guarulhos, SP, Brazil.
authors.forename: Marcos P
authors.initials: MP
authors.lastname: Silva
authors.affiliation: Núcleo de Pesquisa em Doenças Negligenciadas, Guarulhos, SP, Brazil.
authors.forename: Cristiano G
authors.initials: CG
authors.lastname: Oliveira
authors.affiliation: Núcleo de Pesquisa em Doenças Negligenciadas, Guarulhos, SP, Brazil.
authors.forename: Daniela C
authors.initials: DC
authors.lastname: Monteiro
authors.affiliation: Núcleo de Enteroparasitas, Instituto Adolfo Lutz, São Paulo, SP, Brazil.
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Pinto
authors.affiliation: Laboratório de Parasitologia, Instituto Butantan, São Paulo, SP, Brazil.
authors.forename: Ronaldo Z
authors.initials: RZ
authors.lastname: Mendonça
authors.affiliation: Laboratório de Produtos Naturais, Instituto Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Joaquim S
authors.initials: JS
authors.lastname: Costa Júnior
authors.affiliation: Programa de Pós-graduação em Biotecnologia, Rede Nordeste de Biotecnologia, Universidade Federal do Piauí, Teresina, PI, Brazil.
authors.forename: Rivelilson M
authors.initials: RM
authors.lastname: Freitas
authors.affiliation: Núcleo de Pesquisa em Doenças Negligenciadas, Guarulhos, SP, Brazil. Electronic address: josuem@usp.br.
authors.forename: Josué
authors.initials: J
authors.lastname: de Moraes
mesh_terms: D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D028084:Clusiaceae / Q000737:chemistry; D005260:Female; D008297:Male; D008958:Models, Molecular; D009043:Motor Activity / Q000187:drug effects; D012098:Reproduction / Q000187:drug effects; D012550:Schistosoma mansoni / Q000187:drug effects; D012556:Schistosomicides / Q000633:toxicity; D012639:Seeds / Q000737:chemistry; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D014709:Vero Cells
chemical_list: D000977:Antiparasitic Agents; D012556:Schistosomicides; D014315:Triterpenes; C573355:garcinielliptone FC
keywords: Antiparasitic activity; Antischistosomal drug; Confocal laser scanning microscopy; Cytotoxicity; Garcinielliptone FC; Schistosoma