title: Additive Suppression of LPS-Induced IL-10 and TNF-α by Pre-treatment of Dexamethasone and SB203580 in a Murine Alveolar Macrophage Cell Line (MH-S).
abstract: P38 inhibitors are potent anti-inflammatory agents with distinctive mechanism of action from corticosteroid; the potential combined use of both anti-inflammatory agents could be an effective treatment for inflammatory lung disease; however, the impact of such combination on the homeostasis of immune response was poorly understood. To investigate the combined effect of dexamethasone (DEX) and/or SB203580 on tumor necrosis factor (TNF)-α (pro-inflammatory) and interleukin (IL)-10 (anti-inflammatory) secretion in mouse alveolar macrophage cell line. Secreted TNF-α and IL-10 were measured by ELISA. Phosphorylated STAT3 were investigated using Western blotting and immunocytochemistry. Pre-treatment of DEX or SB203580 inhibited lipopolysaccharide (LPS)-stimulated IL-10, TNF-α secretion, and STAT3 phosphorylation. Combined use of both agents showed stronger inhibitory effect. Combining DEX and SB203580 showed strong inhibition on the LPS-induced IL-10 secretion and STAT3 phosphorylation, which might reflect a very important drawback from the combined use of both anti-inflammatory agents.
authors.affiliation: Respiratory Division, The Second Hospital of Hebei Medical University, Heping Western Road 215#, Shijiazhuang City, 050000, Hebei, China, 13833116045@163.com.
authors.forename: Aihong
authors.initials: A
authors.lastname: Meng
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Qi
authors.forename: Dengchang
authors.initials: D
authors.lastname: Liu
authors.forename: Jiakai
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D005938:Glucocorticoids / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology*; D016753:Interleukin-10 / Q000378:metabolism*; D008070:Lipopolysaccharides; D016676:Macrophages, Alveolar / Q000378:metabolism; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology*; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005938:Glucocorticoids; C556062:IL10 protein, mouse; D007093:Imidazoles; D008070:Lipopolysaccharides; D011725:Pyridines; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D003907:Dexamethasone; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580