title: Effect of DAPK1 gene on proliferation, migration, and invasion of carcinoma of pancreas BxPC-3 cell line.
abstract: DAPK1 can induce apoptosis in several cells; to determine the effect of DAPK1 would provide a new potential therapeutic strategy for treating pancreatic cancer. The aim of the present study was to investigate the effect of DAPK1 gene on proliferation, migration, and invasion of carcinoma of pancreas BxPC-3 cell line and explore the possible mechanisms. In our study, DAPK1 over-expressed cells were established by using the lentiviral transfection method, and DAPK1 obviously increased in BxPC-3 cells after transient transfection. Cell Counting Kit-8 (CCK-8) assay was used to determine the BxPC-3 cells proliferation after transfection. Apoptosis of the BxPC-3 cells was determined by using flow cytometry analysis. In addition, cell adhesion assay and in vitro invasion assay were performed. Western blotting was used to determine the protein expressions of caspase-3, DAPK1, VEGF, PEDF, MMP2, AKT, P-AKT, P-ERK, Bcl2, and Bax. Our results demonstrated that DAPK1 gene over-expression can suppress the proliferation, migration, and invasion of carcinoma of pancreas BxPC-3 cell line, and the possible mechanisms may be correlated to induction of mitochondria-mediated apoptosis, down-regulations of MMP-2 and VEGF, up-regulations of PEDF, through the PI3K/Akt and ERK pathways.
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Guan-Xiong
authors.initials: GX
authors.lastname: Ye
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: De-Biao
authors.initials: DB
authors.lastname: Pan
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Jiang
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.affiliation: Department of Hepatobiliary Surgery, People Hospital of Lishui Lishui 323000, China.
authors.forename: Sheng-Qian
authors.initials: SQ
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D064550:Death-Associated Protein Kinases / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D005136:Eye Proteins / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015843:Serpins / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D000099174:Pigment Epithelium-Derived Factor
chemical_list: D053148:Caspase 3; D064550:Death-Associated Protein Kinases; D005136:Eye Proteins; D020778:Matrix Metalloproteinase 2; D009414:Nerve Growth Factors; D051057:Proto-Oncogene Proteins c-akt; D015843:Serpins; D042461:Vascular Endothelial Growth Factor A; D000099174:Pigment Epithelium-Derived Factor; C579096:DAPK1 protein, human
keywords: BxPC-3 cell; DAPK1; carcinoma of pancreas; invasion; migration; proliferation