title: Traceable clonal culture and chemodrug assay of heterogeneous prostate carcinoma PC3 cells in microfluidic single cell array chips.
abstract: Cancer heterogeneity has received considerable attention for its role in tumor initiation and progression, and its implication for diagnostics and therapeutics in the clinic. To facilitate a cellular heterogeneity study in a low cost and highly efficient manner, we present a microfluidic platform that allows traceable clonal culture and characterization. The platform captures single cells into a microwell array and cultures them for clonal expansion, subsequently allowing on-chip characterization of clonal phenotype and response against drug treatments. Using a heterogeneous prostate cancer model, the PC3 cell line, we verified our prototype, identifying three different sub-phenotypes and correlating their clonal drug responsiveness to cell phenotype.
authors.affiliation: Department of Electrical Engineering and Computer Science, University of Michigan , 1301 Beal Avenue, Ann Arbor, Michigan 48109, USA.
authors.forename: Jaehoon
authors.initials: J
authors.lastname: Chung
authors.affiliation: Department of Biomedical Engineering, University of Michigan , 2200 Bonisteel Boulevard, Ann Arbor, Michigan 48109, USA.
authors.forename: Patrick N
authors.initials: PN
authors.lastname: Ingram
authors.forename: Tom
authors.initials: T
authors.lastname: Bersano-Begey
authors.forename: Euisik
authors.initials: E
authors.lastname: Yoon