title: Identification of microRNAs and target genes involved in serous ovarian carcinoma and their influence on survival.
abstract: OBJECTIVE
  To identify the expression of key microRNAs and their predicted target genes involved in serous ovarian carcinoma (SOC) and correlation between miRNAs and prognosis.
  
  
  METHODS
  The authors used quantitative polymerase chain reaction (qPCR) to detect the expression of miR-145, miR-143, miR-146a, miR-93, miR-29a, miR-18a, and miR-200a in tissues of 56 primary SOC patients and 30 benign lesion patients. Four protein (MUC1, FAP, MMP2, MMP9) expressions were identified by western blotting and immunohistochemical stain. The Kaplan-Meier method was used to estimate the relationship of these miRNAs with overall survival rate.
  
  
  RESULTS
  In SOC tissue, expression of miR-200a, miR-93, miR-146a, and miR-18a were up-regulated, while miR-145, miR-143, miR-29a were down-regulated. MUC1, FAP, MMP2, and MMP9 were overexpressed in SOC. MiR-143 or miR-145 higher expressed patients had significantly higher overall survival (OS) rates, while miR-93 or miR-200a lower expressed patients also had higher OS rates.
  
  
  CONCLUSIONS
  These results suggested that several miRNAs and their regulated target transcripts may have important roles in the initiation and development of SOC. MiR-145/miR-143, miR-200a, and miR-93 could be used as prognostic biomarkers.
authors.affiliation: Department of Obstetrics and Gynecology,  Second Hospital of Jilin University, Changchun, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Fang
authors.forename: F
authors.initials: F
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D018284:Cystadenocarcinoma, Serous / Q000235:genetics* / Q000401:mortality; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000032:analysis*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000401:mortality; D011379:Prognosis; D015996:Survival Rate
chemical_list: D035683:MicroRNAs