title: Therapeutic potency of saponin rich aqueous extract of Scoparia dulcis L. in alloxan induced diabetes in rats.
abstract: BACKGROUND
  Diabetes mellitus is major metabolic disorders of carbohydrate metabolism. This leads to alter the multiple organ system.
  
  
  OBJECTIVE
  To investigate the antidiabetic and antioxidant effects of the saponin rich aqueous extract of Scoparia dulcis (SRE-SD) using alloxan-induced hyperglycemic rat model.
  
  
  METHODS
  The single dose of alloxan was injected for the induction of diabetes in rats. The SRE-SD and glibenclamide were administered for 15 consecutive days from the 3(rd) day of alloxan administration. Quantity of food and water intake was measured at day 0, and 18. Further, body weight was recorded and blood samples were collected at different time intervals that is, day 0, 3, 8, 13, and 18. The oxidative biomarkers (i.e. thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH) and nitrite (NO(2-)) levels were also estimated in the serum sample.
  
  
  RESULTS
  The SRE-SD showed a remarkable dose and time-dependent changes in alloxan-induced rise in the level of food consumption and water intake, serum glucose level, TBARS, NO(2-) and fall in the level of GSH. Further, significant attenuation was observed at 20 and 30 mg/kg of SRE-SD treated group.
  
  
  CONCLUSIONS
  These findings demonstrate that SRE-SD has both antidiabetic and antioxidant effects on the experimental model of diabetes in rat.
authors.affiliation: Department of Biotechnology, Udaya School of Engineering, Kanyakumari, Tamil Nadu, India.
authors.forename: P Saravana
authors.initials: PS
authors.lastname: Perumal
authors.affiliation: Department of Biotechnology, Udaya School of Engineering, Kanyakumari, Tamil Nadu, India.
authors.forename: P V
authors.initials: PV
authors.lastname: Anaswara
authors.affiliation: Akal Pharmacology and Toxicology Research Centre, A unit of Akal College of Pharmacy and Technical Education, Sangrur, India.
authors.forename: A
authors.initials: A
authors.lastname: Muthuraman
authors.affiliation: Department of Pharmaceutical Sciences and Drug Research, Pharmacology Division, Council for Scientific and Industrial Research, Punjabi University, Patiala, Punjab, India.
authors.forename: S
authors.initials: S
authors.lastname: Krishan
keywords: Alloxan; Scoparia dulcis; diabetes mellitus; oxidative stress; saponin