title: Tea polyphenols increase X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 expression in the hippocampus of rats during cerebral ischemia/reperfusion injury.
abstract: Recent studies have shown that tea polyphenols can cross the blood-brain barrier, inhibit apoptosis and play a neuroprotective role against cerebral ischemia. Furthermore, tea polyphenols can decrease DNA damage caused by free radicals. We hypothesized that tea polyphenols repair DNA damage and inhibit neuronal apoptosis during global cerebral ischemia/reperfusion. To test this hypothesis, we employed a rat model of global cerebral ischemia/reperfusion. We demonstrated that intraperitoneal injection of tea polyphenols immediately after reperfusion significantly reduced apoptosis in the hippocampal CA1 region; this effect started 6 hours following reperfusion. Immunohistochemical staining showed that tea polyphenols could reverse the ischemia/reperfusion-induced reduction in the expression of DNA repair proteins, X-ray repair cross-complementing protein 1 and apurinic/apyrimidinic endonuclease/redox factor-1 starting at 2 hours. Both effects lasted at least 72 hours. These experimental findings suggest that tea polyphenols promote DNA damage repair and protect against apoptosis in the brain.
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China ; Department of Anesthesiology, Stomatological Hospital, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Rongliang
authors.initials: R
authors.lastname: Xue
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lei
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Jianrui
authors.initials: J
authors.lastname: Lv
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lei
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Anesthesiology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wei
keywords: X-ray repair cross-complementing protein 1; apurinic/apyrimidinic endonuclease/redox factor-1; global cerebral ischemia/reperfusion; tea polyphenols