title: Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-κB in Acute Transection Spinal Cord Injury in Rats.
abstract: OBJECTIVE
  To assess the effect of bone marrow mononuclear cells (BMMNCs) transplantation in the expression of nuclear factor-κB (NF-κB) in spinal cord injury (SCI) in rats.
  
  
  METHODS
  BMMNCs were isolated from tibia and femur by a density gradient centrifugation. After establishment of acute transection SCI, rats were divided into experiment (BMMNCs), experiment control (0.1 M PBS infused) and sham surgery groups (laminectomy without any SCI). Locomotor function was assessed weekly for 5 weeks post-injury using BBB locomotor score and urinary bladder function daily for 4 weeks post-injury. Activity of NF-κB in spinal cord was assessed by immunohistochemistry and reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  At each time point post-injury, sham surgery group had significantly higher Basso, Beattie, Bresnahan locomotor and urinary bladder function scores than experiment and experiment control group (p<0.05). At subsequent time interval there were gradual improvement in both experiment and experiment control group, but experiment group had higher score in comparison to experiment control group (p<0.05). Comparisons were also made for expression of activated NF-κB positive cells and level of NF-κB messenger RNA in spinal cord at various time points between the groups. Activated NF-κB immunoreactivity and level of NF-κB mRNA expression were significantly higher in control group in comparison to experiment and sham surgery group (p<0.05).
  
  
  CONCLUSIONS
  BMMNCs transplantation attenuates the expression of NF-κB in injured spinal cord tissue and thus helps in recovery of neurological function in rat models with SCI.
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Rajiv Prasad
authors.initials: RP
authors.lastname: Shrestha
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Jian Min
authors.initials: JM
authors.lastname: Qiao
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Fu Guo
authors.initials: FG
authors.lastname: Shen
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Krishna Bahadur
authors.initials: KB
authors.lastname: Bista
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Zhong Nan
authors.initials: ZN
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital of Jiamusi University, Heilongjiang, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Yang
keywords: Cell transplantation; Inflammation; Mononuclear cells; NF-κB; Neuroprotective agent; Spinal cord injury