title: Protective effect of a lipid-based preparation from Mycobacterium smegmatis in a murine model of progressive pulmonary tuberculosis.
abstract: A more effective vaccine against tuberculosis (TB) is urgently needed. Based on its high genetic homology with Mycobacterium tuberculosis (Mtb), the nonpathogenic mycobacteria, Mycobacterium smegmatis (Ms), could be an attractive source of potential antigens to be included in such a vaccine. We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb/c mice after challenge with Mtb H37Rv strain. The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb/c mice following challenge with Mtb. Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL) or nonadjuvanted (LMs) showed significant reductions in bacterial load (P < 0.01) compared to the negative control group (animals immunized with phosphate buffered saline (PBS)). Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG). Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group (P < 0.01) and showed similar results compared with the BCG group. These findings suggest the potential of LMs as a promising vaccine candidate against TB.
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Maria de los Angeles
authors.initials: Mde L
authors.lastname: García
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Reinier
authors.identifier: 0000-0003-0679-7973
authors.initials: R
authors.lastname: Borrero
authors.affiliation: Center for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, Cuba.
authors.forename: Maria E
authors.initials: ME
authors.lastname: Lanio
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Yanely
authors.initials: Y
authors.lastname: Tirado
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Nadine
authors.initials: N
authors.lastname: Alvarez
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Alina
authors.initials: A
authors.lastname: Puig
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba.
authors.forename: Alicia
authors.initials: A
authors.lastname: Aguilar
authors.affiliation: Center for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, Cuba.
authors.forename: Liem
authors.initials: L
authors.lastname: Canet
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, Mexico.
authors.forename: Dulce
authors.initials: D
authors.lastname: Mata Espinoza
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Barrios Payán
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba ; School of Health Sciences, Universiti Sains Malaysia, Malaysia.
authors.forename: María Elena
authors.initials: ME
authors.lastname: Sarmiento
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 14000 México, DF, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: School of Health Sciences, Universiti Sains Malaysia, Malaysia ; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Mohd-Nor
authors.initials: MN
authors.lastname: Norazmi
authors.affiliation: Instituto Finlay, 11600 Havana, Cuba ; Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
authors.forename: Armando
authors.initials: A
authors.lastname: Acosta
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000627:therapeutic use; D001500:BCG Vaccine / Q000008:administration & dosage / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D007114:Immunization; D008055:Lipids / Q000008:administration & dosage / Q000276:immunology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D051379:Mice; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D014611:Vaccination
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D008055:Lipids