title: Diagnostic value of plasma signal peptide-Cub-Egf domain-containing protein-1 (SCUBE-1) in an experimental model of acute ischemic stroke.
abstract: OBJECTIVE
  This study was intended to examine possible diagnostic value of plasma Signal Peptide-Cub-Egf domain-containing protein-1 (SCUBE1) levels in an experimental model of acute ischemic stroke.
  
  
  METHODS
  Twenty-four female Sprague Dawley rats were divided into four groups. Blood and brain tissue specimens were collected immediately following artery ligation (control; Group 1), 1h after ligation (Group 2), 2 h after ligation (Group 3) and 6h after ligation (Group 4). SCUBE1 levels were investigated in the serum specimens. The brain samples were examined histopathologically. Correlation analysis was performed between the values.
  
  
  RESULTS
  Median SCUBE1 values were 1.75 ng/ml in the control group, 3.80 ng/ml, 3.71 ng/ml and 4.19 ng/ml in the groups 2, 3 and 4, respectively (n=6 for each, P=0.004, for each group compared to control values). Histopathological analysis revealed median atrophic neuron percentages of 16% (in group 1), 42%, 55% and 76% in group 2, 3 and 4 respectively (n=6 for each, P=0.004, for each group compared to control group). A higly significant correlation was determined between SCUBE-1 levels and percentage of atrophic neurons (r=0.744 P=0.000).
  
  
  CONCLUSIONS
  In this experimental model of acute ischemic stroke plasma SCUBE1 levels rose from the 1st hour of induced stroke and remained high up to 6th hour tested. Results of this experimental study has a potential to become the basis for a clinical study to confirm whether SCUBE1 can be used as a biomarker in the early diagnosis of acute ischemic stroke patients.
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Emergency Medicine, Trabzon, Turkey.
authors.forename: Suha
authors.initials: S
authors.lastname: Turkmen
authors.affiliation: Diyarbakır Selahaddin Eyyubi State Hospital, Department of Emergency Medicine, Diyarbakır, Turkey. Electronic address: umuteryigitacil@gmail.com.
authors.forename: Umut
authors.initials: U
authors.lastname: Eryigit
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Emergency Medicine, Trabzon, Turkey.
authors.forename: Yunus
authors.initials: Y
authors.lastname: Karaca
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Biochemistry, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Mentese
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Biochemistry, Trabzon, Turkey.
authors.forename: Uzun Aysegul
authors.initials: UA
authors.lastname: Sumer
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Histology, Trabzon, Turkey.
authors.forename: Esin
authors.initials: E
authors.lastname: Yulug
authors.affiliation: Manisa State Hospital, Department of Emergency Medicine, Manisa, Turkey.
authors.forename: Nurhak
authors.initials: N
authors.lastname: Aksut
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Neurology, Trabzon, Turkey.
authors.forename: Sibel
authors.initials: S
authors.lastname: Gazioglu
authors.affiliation: Karadeniz Technical University, Faculty of Medicine, Department of Emergency Medicine, Trabzon, Turkey.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Gunduz
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D002545:Brain Ischemia / Q000097:blood* / Q000175:diagnosis / Q000473:pathology; D002352:Carrier Proteins / Q000097:blood*; D004195:Disease Models, Animal; D005260:Female; D008565:Membrane Proteins / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000097:blood* / Q000175:diagnosis / Q000473:pathology
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; C528568:Scube1 protein, rat