title: Molecular characterization of RNA binding motif protein 3 (RBM3) gene from Pashmina goat.
abstract: Pashmina goat inhabits the high altitude cold arid desert of Ladakh, India. This goat is known for its finest and costliest under fiber. Though the under fiber may be a part of its complex thermoregulation mechanism, the genetics of its adaptability under cold conditions is not known. As an attempt to understand its adaptive genetics, and the role of RNA-binding proteins at the cellular response, this study was conducted to characterize the RBM3 gene in Pashmina goat and its expression during hypothermia. The ORF of Pashmina RBM3 gene was 273 bp. Phylogenetic analysis revealed that Pashmina RBM3 is closely related to Bos taurus RBM3. Pashmina RBM3 was characterized by comparative modeling studies. The final 3-D model contained two α-helices and four β-sheets. qRT-PCR data showed that Pashmina RBM3 gene expression was significantly higher (P < 0.05) at moderate (30 °C) hypothermic stress conditions as compared with deep (15 °C) hypothermia.
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Ruby
authors.initials: R
authors.lastname: Zargar
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Uneeb
authors.initials: U
authors.lastname: Urwat
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Firdose
authors.initials: F
authors.lastname: Malik
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Riaz A
authors.initials: RA
authors.lastname: Shah
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: M H
authors.initials: MH
authors.lastname: Bhat
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Niyaz A
authors.initials: NA
authors.lastname: Naykoo
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Firdous
authors.initials: F
authors.lastname: Khan
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: H M
authors.initials: HM
authors.lastname: Khan
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Syed Mudasir
authors.initials: SM
authors.lastname: Ahmed
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir.
authors.forename: Ramesh Kumar
authors.initials: RK
authors.lastname: Vijh
authors.affiliation: Division of Animal Biotechnology, SKUAST-K, Shuhama, Alesteng, Kashmir. Electronic address: naganai@skuastkashmir.ac.in.
authors.forename: Nazir A
authors.initials: NA
authors.lastname: Ganai
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D005786:Gene Expression Regulation / Q000502:physiology*; D006041:Goats / Q000378:metabolism*; D007035:Hypothermia / Q000378:metabolism / Q000662:veterinary*; D007194:India; D008956:Models, Chemical; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
chemical_list: D016601:RNA-Binding Proteins; D012313:RNA
keywords: Comparative modeling; Expression analysis; Hypothermia; Pashmina; RNA binding motif protein 3