title: How FlAsH got its sparkle: historical recollections of the biarsenical-tetracysteine tag.
abstract: The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.
authors.affiliation: Independent Researcher, 4470 Arch Street, San Diego, CA, USA, albert007@cox.net.
authors.forename: B Albert
authors.initials: BA
authors.lastname: Griffin
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Adams
authors.forename: Roger Y
authors.initials: RY
authors.lastname: Tsien
mesh_terms: D001152:Arsenicals / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry*; D049673:History, 20th Century; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D013194:Staining and Labeling / Q000266:history
chemical_list: D001152:Arsenicals; D011993:Recombinant Fusion Proteins; D003545:Cysteine