title: Candida parapsilosis biofilm identification by Raman spectroscopy.
abstract: Colonies of Candida parapsilosis on culture plates were probed directly in situ using Raman spectroscopy for rapid identification of specific strains separated by a given time intervals (up to months apart). To classify the Raman spectra, data analysis was performed using the approach of principal component analysis (PCA). The analysis of the data sets generated during the scans of individual colonies reveals that despite the inhomogeneity of the biological samples unambiguous associations to individual strains (two biofilm-positive and two biofilm-negative) could be made.
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. osamek@isibrno.cz.
authors.forename: Ota
authors.initials: O
authors.lastname: Samek
authors.affiliation: Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic. k.mlynarikova@gmail.com.
authors.forename: Katarina
authors.initials: K
authors.lastname: Mlynariková
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. berns@isibrno.cz.
authors.forename: Silvie
authors.initials: S
authors.lastname: Bernatová
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. jezek@isibrno.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Ježek
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. vlk@isibrno.cz.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Krzyžánek
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. siler@isibrno.cz.
authors.forename: Martin
authors.initials: M
authors.lastname: Šiler
authors.affiliation: Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic, v.v.i., Královopolská 147, Brno 61264, Czech Republic. pavlik@isibrno.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Zemánek
authors.affiliation: Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic. fruzic@fnusa.cz.
authors.forename: Filip
authors.initials: F
authors.lastname: Růžička
authors.affiliation: Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic. veronika.hola@fnusa.cz.
authors.forename: Veronika
authors.initials: V
authors.lastname: Holá
authors.affiliation: Department of Microbiology, Faculty of Medicine and St. Anne's Faculty Hospital, Brno 65691, Czech Republic. martina.mahelova@fnusa.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Mahelová
mesh_terms: D018441:Biofilms; D002175:Candida / Q000145:classification* / Q000166:cytology / Q000648:ultrastructure; D013059:Spectrum Analysis, Raman