title: Comparison of two different methods in the cryopreservation of Eurasian perch (Perca fluviatilis) sperm.
abstract: Two different cryopreservation methods were compared and an optimal dilution ratio for the use of controlled-rate freezer (CRF) was established for Eurasian perch (Perca fluviatilis) sperm. Progressive motility (72 ± 15%) and curvilinear velocity (VCL, 146 ± 11 μm/s) of sperm cryopreserved with CRF did not reduce significantly compared to fresh sperm [progressive motility (90 ± 4%), VCL (173 ± 24 μm/s)]. On the other hand, progressive motility (62 ± 15%) and VCL (120 ± 21 μm/s) of sperm cryopreserved with the conventional floating frame technique were significantly lower when compared to the fresh control. Sperm in both cryopreserved groups showed significantly higher straightness [STR, CRF (84 ± 4%), frame (84 ± 2%)] than in the fresh control group (68 ± 4%). Perch sperm cryopreserved with CRF at a dilution ratio of 1:20 showed significantly higher progressive motility (49 ± 6%) than at a ratio of 1:5 (39 ± 6%) and showed significantly higher VCL (129 ± 11 μm/s) than at dilution ratios of 1:10 (112 ± 17 μm/s) and 1:5 (115 ± 9 μm/s).
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary. Electronic address: Bernath.Gergely@mkk.szie.hu.
authors.forename: G
authors.initials: G
authors.lastname: Bernáth
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: Z
authors.initials: Z
authors.lastname: Bokor
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Kása
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: L
authors.initials: L
authors.lastname: Várkonyi
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: Á
authors.initials: Á
authors.lastname: Hegyi
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: T
authors.initials: T
authors.lastname: Kollár
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: B
authors.initials: B
authors.lastname: Urbányi
authors.affiliation: Department of Lake and River Fisheries, University of Warmia and Mazury, Olsztyn, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Żarski
authors.affiliation: Szabolcsi Halászati Kft., Csillag 16., H-4400 Nyíregyháza, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Radóczi Ifj
authors.affiliation: Department of Aquaculture, Szent István University, Páter Károly 1., H-2100 Gödöllő, Hungary.
authors.forename: Á
authors.initials: Á
authors.lastname: Horváth
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D010471:Perches; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*
keywords: Controlled-rate freezer; Cryopreservation; Eurasian perch; Sperm; Standardization