title: Dehydroxymethylepoxyquinomicin selectively ablates T-CAEBV cells.
abstract: Chronic active Epstein-Barr virus infection (CAEBV) represents a new subtype of lymphoproliferative disorders characterized by high morbidity and mortality rates and often leads to malignant transformation of infected cells. Efficient therapeutic strategies are presently unavailable; therefore, the development of therapies to prevent CAEBV-mediated transformation and disease progression is crucial. Here, we used microarray analysis and luciferase reporter assays to reveal the potential role of activated nuclear factor kappa B (NF-kB) in T cell type of-CAEBV infection. Using a series of cellular and molecular experiments, we demonstrated that dehydroxymethylepoxyquinomicin (DHMEQ), a novel NF-kB inhibitor, can selectively induce apoptosis in SNT-16 cells infected with CAEBV. Mechanistic studies suggested that DHMEQ induces SNT-16 cell apoptosis through NF-kB inhibition coupled with oxidative stress generation. Thus, activated NF-kB could be a new target for CAEBV therapeutics. Owing to its selective targeting ability, DHMEQ may be a candidate for a novel therapeutic regimen to control the progression of CAEBV infections.
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Wen-Tao
authors.initials: WT
authors.lastname: Yang
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Yao
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Tian
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Jin
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Tong-Xin
authors.initials: TX
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D001549:Benzamides / Q000494:pharmacology*; D002478:Cells, Cultured; D002908:Chronic Disease; D003512:Cyclohexanones / Q000494:pharmacology*; D017931:DNA Primers; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D001549:Benzamides; D003512:Cyclohexanones; D017931:DNA Primers; D016328:NF-kappa B; C464444:dehydroxymethylepoxyquinomicin