title: Loss of growth homeostasis by genetic decoupling of cell division from biomass growth: implication for size control mechanisms.
abstract: Growing cells adjust their division time with biomass accumulation to maintain growth homeostasis. Size control mechanisms, such as the size checkpoint, provide an inherent coupling of growth and division by gating certain cell cycle transitions based on cell size. We describe genetic manipulations that decouple cell division from cell size, leading to the loss of growth homeostasis, with cells becoming progressively smaller or progressively larger until arresting. This was achieved by modulating glucose influx independently of external glucose. Division rate followed glucose influx, while volume growth was largely defined by external glucose. Therefore, the coordination of size and division observed in wild-type cells reflects tuning of two parallel processes, which is only refined by an inherent feedback-dependent coupling. We present a class of size control models explaining the observed breakdowns of growth homeostasis.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Hannah
authors.initials: H
authors.lastname: Schmidt-Glenewinkel
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel naama.barkai@weizmann.ac.il.
authors.forename: Naama
authors.initials: N
authors.lastname: Barkai
mesh_terms: D059447:Cell Cycle Checkpoints / Q000235:genetics; D002455:Cell Division / Q000235:genetics*; D005947:Glucose / Q000378:metabolism; D006706:Homeostasis; D008962:Models, Theoretical; D012331:RNA, Fungal / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics; D017423:Sequence Analysis, RNA
chemical_list: D012331:RNA, Fungal; D005947:Glucose
keywords: cell size control; external vs. internal signalling; glucose signalling; microfluidics