title: The parasitic worm product ES-62 targets myeloid differentiation factor 88-dependent effector mechanisms to suppress antinuclear antibody production and proteinuria in MRL/lpr mice.
abstract: OBJECTIVE
  The hygiene hypothesis suggests that parasitic helminths (worms) protect against the development of autoimmune disease via a serendipitous side effect of worm-derived immunomodulators that concomitantly promote parasite survival and limit host pathology. The aim of this study was to investigate whether ES-62, a phosphorylcholine-containing glycoprotein secreted by the filarial nematode Acanthocheilonema viteae, protects against kidney damage in an MRL/lpr mouse model of systemic lupus erythematosus (SLE).
  
  
  METHODS
  MRL/lpr mice progressively produce high levels of autoantibodies, and the resultant deposition of immune complexes drives kidney pathology. The effects of ES-62 on disease progression were assessed by measurement of proteinuria, assessment of kidney histology, determination of antinuclear antibody (ANA) production and cytokine levels, and flow cytometric analysis of relevant cellular populations.
  
  
  RESULTS
  ES-62 restored the disrupted balance between effector and regulatory B cells in MRL/lpr mice by inhibiting plasmablast differentiation, with a consequent reduction in ANA production and deposition of immune complexes and C3a in the kidneys. Moreover, by reducing interleukin-22 production, ES-62 may desensitize downstream effector mechanisms in the pathogenesis of kidney disease. Highlighting the therapeutic importance of resetting B cell responses, adoptive transfer of purified splenic B cells from ES-62-treated MRL/lpr mice mimicked the protection afforded by the helminth product. Mechanistically, this reflects down-regulation of myeloid differentiation factor 88 expression by B cells and also kidney cells, resulting in inhibition of pathogenic cross-talk among Toll-like receptor-, C3a-, and immune complex-mediated effector mechanisms.
  
  
  CONCLUSIONS
  This study provides the first demonstration of protection against kidney pathology by a parasitic worm-derived immunomodulator in a model of SLE and suggests therapeutic potential for drugs based on the mechanism of action of ES-62.
authors.affiliation: University of Glasgow, Glasgow, UK.
authors.forename: David T
authors.initials: DT
authors.lastname: Rodgers
authors.forename: Mairi A
authors.initials: MA
authors.lastname: McGrath
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Pineda
authors.forename: Lamyaa
authors.initials: L
authors.lastname: Al-Riyami
authors.forename: Justyna
authors.initials: J
authors.lastname: Rzepecka
authors.forename: Felicity
authors.initials: F
authors.lastname: Lumb
authors.forename: William
authors.initials: W
authors.lastname: Harnett
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Harnett
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D004195:Disease Models, Animal; D015801:Helminth Proteins / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008180:Lupus Erythematosus, Systemic / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D019463:Mice, Inbred MRL lpr; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism*; D011507:Proteinuria / Q000378:metabolism* / Q000473:pathology
chemical_list: D000974:Antibodies, Antinuclear; C435884:ES-62 protein, Acanthocheilonema viteae; D015801:Helminth Proteins; D053594:Myeloid Differentiation Factor 88