title: Morphological changes of radial glial cells during mouse embryonic development.
abstract: During brain development, the radial glial cell acts as a scaffold to support radial migration of postmitotic neurons. However, the morphological changes of radial glial cells during embryo development are poorly understood. We used in utero electroporation and immunohistochemistry to study the dynamics of radial glial cells accompanied by cortical development in mice from embryonic day 14 to postnatal day 0. We found that different segments of radial glial cells changed by the growth of different layers of cortex, such as marginal zone, cortical plate, intermediate zone and ventricular zone. Moreover, the length, angle and number of branches of the radial glial cell changed significantly at the late stage of neurogenesis. All these changes were consistent with the distinct phases of locomotion. Thus, we speculated that morphological changes of the radial glial cell were associated with the neuronal migration and dendritic development.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Duan
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Lingzhen
authors.initials: L
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Xinde
authors.initials: X
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China.
authors.forename: Shanting
authors.initials: S
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, P.R. China. Electronic address: cslin9719@nwsuaf.edu.cn.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology* / Q000196:embryology*; D018274:Electroporation; D063928:Ependymoglial Cells / Q000166:cytology*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin / Q000378:metabolism; D055495:Neurogenesis / Q000502:physiology
chemical_list: C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin
keywords: Glial branch; In utero electroporation; Neuronal migration; Radial glial cell