title: Active site-directed proteomic probes for adenylation domains in nonribosomal peptide synthetases.
abstract: We describe a general strategy for selective chemical labeling of individual adenylation (A) domains in nonribosomal peptide synthetases (NRPSs) using active site-directed proteomic probes coupled to the 5'-O-N-(aminoacyl)sulfamoyladenosine (AMS) scaffold with a clickable benzophenone functionality. These proteomic tools can greatly facilitate the molecular identification, functional characterization, and profiling of virtually any kind of A domains of NRPS enzymes in complex biological systems.
authors.affiliation: Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. fishika@pharm.kyoto-u.ac.jp scseigyo-hisyo@pharm.kyoto-u.ac.jp.
authors.forename: Sho
authors.initials: S
authors.lastname: Konno
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Ishikawa
authors.forename: Takehiro
authors.initials: T
authors.lastname: Suzuki
authors.forename: Naoshi
authors.initials: N
authors.lastname: Dohmae
authors.forename: Michael D
authors.initials: MD
authors.lastname: Burkart
authors.forename: Hideaki
authors.initials: H
authors.lastname: Kakeya
mesh_terms: D000241:Adenosine / Q000737:chemistry* / Q000378:metabolism; D001577:Benzophenones / Q000737:chemistry / Q000378:metabolism; D020134:Catalytic Domain; D057930:Click Chemistry; D010453:Peptide Synthases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D001577:Benzophenones; D011994:Recombinant Proteins; C047723:benzophenone; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase; D000241:Adenosine