title: Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
abstract: DH82 cells represent a permanent macrophage cell line isolated from a dog with histiocytic sarcoma (HS) and are commonly used in various fields of research upon infection and cancer, respectively. Despite its frequent use, data on cell surface antigen expression of this cell line are fragmentary and in part inconsistent. We therefore aimed at a detailed morphological and antigenic characterization of DH82 cells with respect to passage-dependent differences. Cellular morphology of early (≤ 13) and late (≥ 66) passages of DH82 cells was evaluated via scanning electron microscopy. Moreover, cells were labelled with 10 monoclonal antibodies directed against CD11c, CD14, CD18, CD44, CD45, CD80, CD86, MHC-I, MHC-II, and ICAM-1 for flow cytometric analysis. Early passage cells were characterized by round cell bodies with abundant small cytoplasmic projections whereas later passages exhibited a spindle-shaped morphology with large processes. The percentage of CD11c-, CD14-, CD18-, CD45-, and CD80 positive cells significantly decreased in late passages whereas the expression of CD44, CD86, MHC-I, MHC-II and ICAM-1 remained unchanged. DH82 cells represent a remarkably heterogeneous cell line with divergent antigenic and morphologic properties. The present findings have important implications for future studies, which should consider distinct characteristics with regard to the used passage.
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559 Hannover, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Heinrich
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559 Hannover, Germany.
authors.forename: Vanessa Bono
authors.initials: VB
authors.lastname: Contioso
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Veronika M
authors.initials: VM
authors.lastname: Stein
authors.affiliation: Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Carlson
authors.affiliation: Center for Systems Neuroscience, Bünteweg 2, 30559 Hannover, Germany; Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover Foundation, Bünteweg 9, 30559 Hannover, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tipold
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Ulrich
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Puff
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559 Hannover, Germany. Electronic address: Wolfgang.Baumgaertner@tiho-hannover.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Baumgärtner
authors.affiliation: Department of Pathology, University of Veterinary Medicine Hannover Foundation, Bünteweg 17, 30559 Hannover, Germany; Center for Systems Neuroscience, Bünteweg 2, 30559 Hannover, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Spitzbarth
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000276:immunology / Q000473:pathology*; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D006644:Histiocytes / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D054747:Histiocytic Sarcoma / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D018625:Microscopy, Atomic Force / Q000662:veterinary
chemical_list: D015703:Antigens, CD
keywords: DH82 cells; Flow cytometry; Histiocytic sarcoma; Macrophage cell line; Passage-dependency; Scanning electron microscopy