title: Selection by phage display of nanobodies directed against hypoxia inducible factor-1α (HIF-1α).
abstract: Hypoxia, which promotes tumor invasion and metastasis, is a common phenomenon in solid tumors. Hypoxia generally leads to a higher expression level of hypoxia inducible factor-1 (HIF-1) in tumors (cells) relative to normal tissues (cells). Given the unique expression of HIF-1α in human cancers and its vital importance in mediating hypoxic adaptation, we have identified 20 different HIF-1α-specific nanobodies by using a llama-derived nonimmune phage display library. PAS-B domain of HIF-1α (HIF-1α-PAS-B) has been used as an antigen. Nanobody (VHH16) was selected from these 20 nanobodies by phage enzyme-linked immunosorbent assay. The preliminary analysis of biological activity demonstrates that VHH16 can specifically bind to HIF-1α with high affinity. VHH16 is the first nanobody that specifically binds to HIF-1α-PAS-B as well. We suggest here that VHH16 is useful in disease diagnosis and also has potential in medical applications.
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People's Republic of China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People's Republic of China.
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People's Republic of China.|Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D002161:Camelids, New World; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods*; D017434:Protein Structure, Tertiary; D061905:Single-Domain Antibodies / Q000276:immunology*
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D019151:Peptide Library; D061905:Single-Domain Antibodies
keywords: ELISA; PAS-B; anti-HIF-1α; nanobodies; phage display technology