title: [Coculture of stem cells from the apical papilla and human umbilical vein endothelial cells enhances the angiogenic potential of dental pulp tissues].
abstract: OBJECTIVE
  To observe the effect of coculture of stem cells from the apical papilla (SCAPs) and human umbilical vein endothelial cells (HUVECs) on the angiogenic potential of dental pulp tissues.
  
  
  METHODS
  SCAPs were incubated in osteo/odontogenic, adipogenic, neurogenic induction medium and α-MEM medium, whose multilineage differentiation capacities were confirmed using alizarin red staining, oil red O staining and βIII-tubulin immunofluorescent staining. The tubular length, branching points number and junctional areas were detected after 3, 6, 9 h since cells were seeded onto matrigel, and the data was analyzed using SPSS 16.0 software package.
  
  
  RESULTS
  SCAPs in the experimental groups were detected having more lipid droplets, mineralization nodules and neuron-like cells. Coculture of SCAPs and HUVECs formed more vessel-like structures in tubular formation assay.
  
  
  CONCLUSIONS
  SCAPs are capable of differentiating into fat, bone, and nerve-like cells in vitro. Coculture of SCAPs and HUVECs can enhance the angiogenic potential of dental pulp tissues.
authors.affiliation: Dental Implant Center, Xuzhou Medical College Affiliated Xuzhou Stomatological Hospital. Xuzhou 221002, Jiangsu Province; China.E-mail:cyyuan@hku.hk.
authors.forename: Chang-yong
authors.initials: CY
authors.lastname: Yuan
authors.forename: Peng-lai
authors.initials: PL
authors.lastname: Wang
authors.forename: Cheng-fei
authors.initials: CF
authors.lastname: Zhang
authors.forename: Zong-xiang
authors.initials: ZX
authors.lastname: Liu
authors.forename: San-lian
authors.initials: SL
authors.lastname: Luo
mesh_terms: D000469:Alkaline Phosphatase; D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D003771:Dental Papilla; D003782:Dental Pulp; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D018919:Neovascularization, Physiologic; D009805:Odontogenesis; D009930:Organic Chemicals; D013234:Stem Cells
chemical_list: D009930:Organic Chemicals; C420642:alpha minimal essential medium; D000469:Alkaline Phosphatase