title: Is hydroxyethyl starch necessary for sedimentation of bone marrow?
abstract: Hydroxyethyl starch (HES) is used to separate hematopoietic progenitor cells after bone marrow (BM) collection from red blood cells. The aims were to study alternatives for HAES-steril (200 kDa; not available anymore) and to optimize the sedimentation process. Using WBC-enriched product (10 × 10(9) WBC/L), instead of BM, sedimentation at 10% hematocrit using final 0.6 or 0.39% Voluven (130 kDa) or without HES appeared to be good alternatives for 0.6% HAES-steril. MNC recovery >80% and RBC depletion >90% was reached. Optimal sedimentation was reached using 110-140 mL volume. Centrifugation appeared not suitable for sedimentation. Additional testing with BM might be necessary to confirm these results.
authors.affiliation: Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands. Electronic address: m.dijkstra@sanquin.nl.
authors.forename: Margriet J
authors.initials: MJ
authors.lastname: Dijkstra-Tiekstra
authors.affiliation: Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands.
authors.forename: Airies C
authors.initials: AC
authors.lastname: Setroikromo
authors.affiliation: Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands.
authors.forename: Marcha
authors.initials: M
authors.lastname: Kraan
authors.affiliation: Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands.
authors.forename: Effimia
authors.initials: E
authors.lastname: Gkoumassi
authors.affiliation: Department Transfusion Monitoring, Sanquin Blood Supply, P.O. Box 1191, 9701 BD Groningen, The Netherlands.
authors.forename: Janny
authors.initials: J
authors.lastname: de Wildt-Eggen
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology*; D002469:Cell Separation; D002498:Centrifugation; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000737:chemistry*; D008297:Male; D010952:Plasma Substitutes / Q000737:chemistry*
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes
keywords: Bone marrow HPC; Centrifugation; Gravity; Hydroxyethyl starch; MNC recovery; RBC depletion; Volume; WBC-enriched product