title: Porous membrane culture method for expansion of human pluripotent stem cells.
abstract: For the clinical application of human pluripotent stem cells (hPSCs), it is critical to develop novel culture techniques that completely exclude the use of animal feeder cells and enzyme treatments used in conventional hPSC culture systems. Here, we describe a novel culture method using a porous membrane that allows to maintain stable attachment and expansion of hPSCs, obviates the need for enzyme treatment, and also reduces feeder layer contamination.
authors.affiliation: Department of Biomedical Science, CHA University, CHA Bio Complex, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 463-400, Republic of Korea.
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Seung-Taeh
authors.initials: ST
authors.lastname: Hwang
authors.forename: Soo-Hong
authors.initials: SH
authors.lastname: Lee
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D061252:Feeder Cells; D006801:Humans; D008567:Membranes, Artificial; D039904:Pluripotent Stem Cells / Q000166:cytology*
chemical_list: D008567:Membranes, Artificial