title: Proteomic analysis and functional characterization of mouse brain mitochondria during aging reveal alterations in energy metabolism.
abstract: Mitochondria are the main cellular source of reactive oxygen species and are recognized as key players in several age-associated disorders and neurodegeneration. Their dysfunction has also been linked to cellular aging. Additionally, mechanisms leading to the preservation of mitochondrial function promote longevity. In this study we investigated the proteomic and functional alterations in brain mitochondria isolated from mature (5 months old), old (12 months old), and aged (24 months old) mice as determinants of normal "healthy" aging. Here the global changes concomitant with aging in the mitochondrial proteome of mouse brain analyzed by quantitative mass-spectrometry based super-SILAC identified differentially expressed proteins involved in several metabolic pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Despite these changes, the bioenergetic function of these mitochondria was preserved. Overall, this data indicates that proteomic changes during aging may compensate for functional defects aiding in preservation of mitochondrial function. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium with the data set identifier PXD001370 (http://proteomecentral.proteomexchange.org/dataset/PXD001370).
authors.affiliation: Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Stauch
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Purnell
authors.forename: Lance M
authors.initials: LM
authors.lastname: Villeneuve
authors.forename: Howard S
authors.initials: HS
authors.lastname: Fox
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000502:physiology*; D004734:Energy Metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D015533:Transcriptional Activation
chemical_list: D024101:Mitochondrial Proteins; D020543:Proteome
keywords: Aging; Bioenergetics; Brain mitochondria; Quantitative proteomics