title: Markers of macromolecular oxidative damage in maternal serum and risk of neural tube defects in offspring.
abstract: Neural tube defects (NTDs) are among the most common and severe congenital malformations. To examine the association between markers of macromolecular oxidative damage and risk of NTDs, we measured levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), protein carbonyl (PC), and 8-iso-prostaglandin F2α (8-iso-PGF2α) in maternal serum samples of 117 women with NTD-affected pregnancies and 121 women with healthy term newborns. We found higher levels of 8-OHdG and PC in the NTD group than in the control group; however, we did not observe a statistically significant difference in 8-iso-PGF2α levels between the NTD and the control groups. NTD risk increased with increasing quartiles of 8-OHdG [odds ratio (OR)=1.17; 95% confidence interval (CI) 0.39-3.51; OR=2.19; 95% CI, 0.68-7.01; OR=3.70; 95% CI, 1.30-10.51, for the second, third, and fourth quartile relative to the lowest quartile, respectively; P=0.009], and with increasing quartiles of PC (OR=2.26; 95% CI, 0.66-7.69; OR=3.86; 95% CI, 1.17-12.80; OR=5.98; 95% CI, 1.82-19.66, for the second, third, and fourth quartile relative to the lowest quartile, respectively; P=0.002]. Serum levels of 8-OHdG were higher in women who did not take folic acid supplements during the periconceptional period. These results suggest that oxidative stress is present in women carrying pregnancies affected by NTDs.
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Jufen
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Reproductive and Child Health/Ministry of Health Key Laboratory of Reproductive Health, Peking University, Beijing 100191, China; Department of Epidemiology and Health Statistics, School of Public Health, Peking University, Beijing 100191, China. Electronic address: renag@pku.edu.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Ren
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002681:China; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D019587:Dietary Supplements; D015237:Dinoprost / Q000031:analogs & derivatives / Q000097:blood; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D006771:Hospitals, Maternity; D006801:Humans; D009436:Neural Tube Defects / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D016017:Odds Ratio; D018384:Oxidative Stress; D011159:Population Surveillance; D050050:Protein Carbonylation; D012306:Risk
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005492:Folic Acid; D015237:Dinoprost; D003849:Deoxyguanosine
keywords: 8-Hydroxy-2′-deoxyguanosine; 8-iso-prostaglandin F2α; Neural tube defects; Oxidative stress; Protein carbonyl