title: Effects of angiotensin type 2 receptor on secretion of the locus coeruleus in stress-induced hypertension rats.
abstract: Locus coeruleus (LC) has noradrenergic nerve terminals projecting to hypothalamus that modulating cardiovascular activity. To study the dynamic characteristics of norepinephrine (NE) release in hypothalamus followed by electrical stimulation in the locus coeruleus in the stress-induced hypertension (SIH) rats, we established the hypertension model rats by stimulations combining noise and foot-shock stress. After the end of modeling, NE release in the hypothalamus by electrical stimulation in LC was studied and NE signal was recorded by carbon fiber electrode. The peak value, the time to peak and half-life period of NE signal in both group rats were analyzed. Furthermore, to clarify the role of angiotensin II type 2 receptors (AT2) in norepinephrine (NE) release and the blood pressure of rat model of stress-induced hypertension, we intraperitoneally administered the AT2 receptor antagonist PD123319 (AT2 receptor antagonist, 0.3mg/kg, i.p.) and intracerebroventricularly injection of CGP42112 (AT2 receptor agonist, 6μg/5μl, i.c.v.) to adult male rats. We found the peak value of NE signal in the hypothalamus followed by electrical stimulation in the LC in SIH rats were higher than that in controls (P<0.01). Intraperitoneal injection of PD123319 (AT2 receptor antagonist) potentiated electrical stimulation in the LC induced NE release in the hypothalamus in SIH rats and elevated blood pressure (P<0.05), whereas intracerebroventricular injection of CGP42112 (AT2 receptor agonist) inhibited the NE release and reduced the heart rate (P<0.05). These results suggest that combining noise and foot-shock stresses increased the blood pressure and the secretion of NE in the hypothalamus followed by electrical stimulation in the LC in rats. AT2 receptors can inhibit the secretion of NE from the LC to the hypothalamus. The attenuation of presynaptic action of AT2 receptor may play a role in the pathophysiological mechanism of SIH in rats.
authors.affiliation: Department of Physiology and Neurobiology, Wannan Medical College, Wuhu, Anhui 241002, China.
authors.forename: Wan-kun
authors.initials: WK
authors.lastname: Gong
authors.affiliation: Department of Biochemistry, Wannan Medical College, Wuhu, Anhui 241002, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lü
authors.affiliation: Department of Forensic Medicine, Wannan Medical College, Wuhu, Anhui 241002, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Physiology and Neurobiology, Wannan Medical College, Wuhu, Anhui 241002, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Physiology and Neurobiology, Wannan Medical College, Wuhu, Anhui 241002, China.
authors.forename: Meng-ya
authors.initials: MY
authors.lastname: Wang
authors.affiliation: Department of Physiology and Neurobiology, Wannan Medical College, Wuhu, Anhui 241002, China. Electronic address: huanghp@wnmc.edu.cn.
authors.forename: Hong-ping
authors.initials: HP
authors.lastname: Huang
mesh_terms: D057912:Angiotensin II Type 2 Receptor Blockers / Q000494:pharmacology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D004195:Disease Models, Animal; D006973:Hypertension / Q000503:physiopathology*; D007031:Hypothalamus / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D008125:Locus Coeruleus / Q000378:metabolism* / Q000503:physiopathology*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044139:Receptor, Angiotensin, Type 2 / Q000819:agonists / Q000502:physiology*; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D057912:Angiotensin II Type 2 Receptor Blockers; D007093:Imidazoles; D009842:Oligopeptides; D011725:Pyridines; D044139:Receptor, Angiotensin, Type 2; C060894:CGP 42112A; C073402:PD 123319; D009638:Norepinephrine
keywords: AT(2) receptor; Carbon fiber electrode; Hypothalamus; Locus coeruleus; Stress-induced hypertension