title: Inferred metagenomic comparison of mucosal and fecal microbiota from individuals undergoing routine screening colonoscopy reveals similar differences observed during active inflammation.
abstract: The mucosal microbiota lives in close proximity with the intestinal epithelium and may interact more directly with the host immune system than the luminal/fecal bacteria. The availability of nutrients in the mucus layer of the epithelium is also very different from the gut lumen environment. Inferred metagenomic analysis for microbial function of the mucosal microbiota is possible by PICRUSt. We recently found that by using this approach, actively inflamed tissue of ulcerative colitis (UC) patients have mucosal communities enriched for genes involved in lipid and amino acid metabolism, and reduced for carbohydrate and nucleotide metabolism. Here, we find that the same bacterial taxa (e.g. Acinetobacter) and predicted microbial pathways enriched in actively inflamed colitis tissue are also enriched in the mucosa of subjects undergoing routine screening colonoscopies, when compared with paired samples of luminal/fecal bacteria. These results suggest that the mucosa of healthy individuals may be a reservoir of aerotolerant microbial communities expanded during colitis.
authors.affiliation: a Department of Microbiology ; New York University School of Medicine ; New York , NY USA.
authors.forename: Mei San
authors.initials: MS
authors.lastname: Tang
authors.forename: Jordan
authors.initials: J
authors.lastname: Poles
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Leung
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Wolff
authors.forename: Michael
authors.initials: M
authors.lastname: Davenport
authors.forename: Soo Ching
authors.initials: SC
authors.lastname: Lee
authors.forename: Yvonne Al
authors.initials: YA
authors.lastname: Lim
authors.forename: Kek Heng
authors.initials: KH
authors.lastname: Chua
authors.forename: P'ng
authors.initials: P
authors.lastname: Loke
authors.forename: Ilseung
authors.initials: I
authors.lastname: Cho
mesh_terms: D058448:Biota; D003092:Colitis / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D008875:Middle Aged
keywords: acinetobacter; colitis; inflammation; microbiome; mucosa; proteobacteria