title: Pea fiber and wheat bran fiber show distinct metabolic profiles in rats as investigated by a 1H NMR-based metabolomic approach.
abstract: This study aimed to examine the effect of pea fiber (PF) and wheat bran fiber (WF) supplementation in rat metabolism. Rats were assigned randomly to one of three dietary groups and were given a basal diet containing 15% PF, 15% WF, or no supplemental fiber. Urine and plasma samples were analyzed by NMR-based metabolomics. PF significantly increased the plasma levels of 3-hydroxybutyrate, and myo-inositol as well as the urine levels of alanine, hydroxyphenylacetate, phenylacetyglycine, and α-ketoglutarate. However, PF significantly decreased the plasma levels of isoleucine, leucine, lactate, and pyruvate as well as the urine levels of allantoin, bile acids, and trigonelline. WF significantly increased the plasma levels of acetone, isobutyrate, lactate, myo-inositol, and lipids as well as the urine levels of alanine, lactate, dimethylglycine, N-methylniconamide, and α-ketoglutarate. However, WF significantly decreased the plasma levels of amino acids, and glucose as well as the urine levels of acetate, allantoin, citrate, creatine, hippurate, hydroxyphenylacetate, and trigonelline. Results suggest that PF and WF exposure can promote antioxidant activity and can exhibit common systemic metabolic changes, including lipid metabolism, energy metabolism, glycogenolysis and glycolysis metabolism, protein biosynthesis, and gut microbiota metabolism. PF can also decrease bile acid metabolism. These findings indicate that different fiber diet may cause differences in the biofluid profile in rats.
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Fang
authors.affiliation: Japan International Research Center for Agricultural Sciences, 1-1 sukuba, Ohwashi, TIbaragi, 305-8686, Japan.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu, 611130, Sichuan, China.
authors.forename: Caimei
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D004043:Dietary Fiber / Q000378:metabolism*; D004734:Energy Metabolism; D005260:Female; D050261:Glycogenolysis; D006019:Glycolysis; D050356:Lipid Metabolism; D055442:Metabolome; D018532:Pisum sativum / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014908:Triticum / Q000737:chemistry*