title: Identification of new players in cell division, DNA damage response, and morphogenesis through construction of Schizosaccharomyces pombe deletion strains.
abstract: Many fundamental biological processes are studied using the fission yeast, Schizosaccharomyces pombe. Here we report the construction of a set of 281 haploid gene deletion strains covering many previously uncharacterized genes. This collection of strains was tested for growth under a variety of different stress conditions. We identified new genes involved in DNA metabolism, completion of the cell cycle, and morphogenesis. This subset of nonessential gene deletions will add to the toolkits available for the study of biological processes in S. pombe.
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Chen
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Janel R
authors.initials: JR
authors.lastname: Beckley
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: McDonald
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: MariaSanta
authors.initials: M
authors.lastname: Mangione
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Sylvia J
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Zachary C
authors.initials: ZC
authors.lastname: Elmore
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Nicole
authors.initials: N
authors.lastname: Rachfall
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Anna
authors.initials: A
authors.lastname: Feoktistova
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Jones
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Alaina H
authors.initials: AH
authors.lastname: Willet
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Guillen
authors.affiliation: Department of Genetics, Evolution and Environment and UCL Cancer Institute, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Danny A
authors.initials: DA
authors.lastname: Bitton
authors.affiliation: Department of Genetics, Evolution and Environment and UCL Cancer Institute, University College London, London WC1E 6BT, United Kingdom.
authors.forename: Jürg
authors.initials: J
authors.lastname: Bähler
authors.affiliation: Genome Technology Center, Stanford University School of Medicine, Palo Alto, California 01605.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Jensen
authors.affiliation: Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605.
authors.forename: Nick
authors.initials: N
authors.lastname: Rhind
authors.affiliation: Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 kathy.gould@vanderbilt.edu.
authors.forename: Kathleen L
authors.initials: KL
authors.lastname: Gould
mesh_terms: D002455:Cell Division / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics*; D017353:Gene Deletion; D005800:Genes, Fungal; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics* / Q000378:metabolism
keywords: DNA metabolism; cell division; fission yeast; gene deletion; stress sensitivity