title: Interaction between 5-HT1B receptors and nitric oxide in zebrafish responses to novelty.
abstract: Nitric oxide (NO) and serotonin (5-HT) interact at the molecular and systems levels to control behavioral variables, including agression, fear, and reactions to novelty. In zebrafish, the 5-HT1B receptor has been implicated in anxiety and reactions to novelty, while the 5-HT1A receptor is associated with anxiety-like behavior; this role of the 5-HT1A receptor is mediated by NO. This work investigated whether NO also participates in the mediation of novelty responses by the 5-HT1B receptor. The 5-HT1B receptor inverse agonist SB 224,289 decreased bottom-dwelling and erratic swimming in zebrafish; the effects on bottom-dwelling, but not on erratic swimming, were blocked by pre-treatment with the nitric oxide synthase inhibitor L-NAME. These effects underline a novel mechanism by which 5-HT controls zebrafish reactivity to novel environments, with implications for the study of neotic reactions, exploratory behavior, and anxiety-like states.
authors.affiliation: Laboratório de Neurociências e Comportamento "Frederico Guilherme Graeff", Universidade do Estado do Pará, Marabá, PA, Brazil; International Zebrafish Neuroscience Research Consortium, Brazil. Electronic address: caio.maximino@uepa.br.
authors.forename: Caio
authors.initials: C
authors.lastname: Maximino
authors.affiliation: Laboratório de Neurociências e Comportamento "Frederico Guilherme Graeff", Universidade do Estado do Pará, Marabá, PA, Brazil; International Zebrafish Neuroscience Research Consortium, Brazil; Laboratório de Neuroendocrinologia, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Monica Gomes
authors.initials: MG
authors.lastname: Lima
authors.affiliation: Laboratório de Neuroendocrinologia, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Evander de Jesus Oliveira
authors.initials: Ede J
authors.lastname: Batista
authors.affiliation: Laboratório de Neuroendocrinologia, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Karen Renata Herculano Matos
authors.initials: KR
authors.lastname: Oliveira
authors.affiliation: International Zebrafish Neuroscience Research Consortium, Brazil; Laboratório de Neuroendocrinologia, Universidade Federal do Pará, Belém, PA, Brazil.
authors.forename: Anderson Manoel
authors.initials: AM
authors.lastname: Herculano
mesh_terms: D000818:Animals; D054314:Drug Inverse Agonism; D005106:Exploratory Behavior; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010881:Piperidones / Q000494:pharmacology; D044302:Receptor, Serotonin, 5-HT1B / Q000378:metabolism*; D013141:Spiro Compounds / Q000494:pharmacology; D015027:Zebrafish / Q000502:physiology*
chemical_list: D010881:Piperidones; D044302:Receptor, Serotonin, 5-HT1B; C108518:SB 22489G; D013141:Spiro Compounds; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester
keywords: Anxiety; Nitric oxide; Novelty; Serotonin; Zebrafish