title: Biological features of biofilm-forming ability of Acinetobacter baumannii strains derived from 121 elderly patients with hospital-acquired pneumonia.
abstract: This study is to investigate a biological activity of Acinetobacter baumannii isolates from sputum specimens of 121 elderly patients with hospital-acquired pneumonia. The ability of the isolates to form biofilms was quantitatively assessed by crystal violet staining, and adhesive property was examined using Giemsa staining. Biofilm-forming ability by the isolates was employed to test antimicrobial resistance and examine sources and clinical manifestations. The isolates grew as biofilm on abiotic surface at the indicated temperatures after a 48 h of incubation. 27.3 % of the isolates were strongly biofilm-positive in the samples, and 84.8 % displayed high adhesion ability (P < 0.05). All of the isolates showed antibiotic resistance at different levels, and the isolates produced strong biofilm exhibited low-level resistance to gentamicin, minocycline and ceftazidime (P < 0.05). The patients' experience in ICU, use of antibiotics and estimation of APACHE II (<17) were related to incidence of strong biofilm formation with no clinical manifestations found in the study. All clinical isolates are able to form biofilms which refer to adhesive efficiency and antibiotic resistance. Patient experiences in ICU surveillance, use of antibiotics and APACHE II scores are involved in biofilm-forming ability by the nosocomial pathogen derived from the hospitalized patients.
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing, 100853, China.|Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Duchao
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing, 100853, China.|Department of Respiratory Diseases, Huai'an First People's Hospital, Huai'an, 223300, Jiangsu, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xia
authors.affiliation: Nanlou Clinical Laboratory Department, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Nanlou Clinical Laboratory Department, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Meiliang
authors.initials: M
authors.lastname: Gong
authors.affiliation: Nanlou Clinical Laboratory Department, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Xie
authors.affiliation: Nanlou Respiratory Diseases Department, Chinese PLA General Hospital, 28 Fuxing Rd, Beijing, 100853, China. fangxq301@126.com.
authors.forename: Xiangqun
authors.initials: X
authors.lastname: Fang
mesh_terms: D040981:Acinetobacter baumannii / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology; D001422:Bacterial Adhesion; D018441:Biofilms / Q000187:drug effects; D002460:Cell Line; D003428:Cross Infection / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D007362:Intensive Care Units; D008826:Microbial Sensitivity Tests; D011014:Pneumonia / Q000382:microbiology*; D013183:Sputum / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents
keywords: Acinetobacter baumannii; Adhesion ability; Antibiotic susceptibility; Biofilm-forming ability