title: Determination of thailandepsin B in rat plasma with a LC-MS/MS method.
abstract: BACKGROUND
  Thailandepsin B (TDP-B) is a potent histone deacetylase inhibitor under development. A reliable bioanalytical method for the quantification of TDP-B in plasma samples is required.
  
  
  RESULTS
  The stabilizer mixture containing hydrochloric acid, formic acid and dichlorvos was applied to stabilize TDP-B in matrix samples. The method validation was conducted over the curve range of 1.00 to 1000 ng/ml. The intrabatch and interbatch precision and accuracy of the quality control samples showed ≤10.9% relative standard deviation and -6.7% to 15.0% relative error. Moreover, a possible metabolite M of TDP-B was tentatively characterized.
  
  
  CONCLUSIONS
  A reliable LC-MS/MS method was developed and validated for the quantification of TDP-B and was successfully applied to a rat pharmacokinetic study.
authors.affiliation: Tongji School of Pharmacy, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan, Hubei 430030, China.
authors.forename: Zhongzhe
authors.initials: Z
authors.lastname: Cheng
authors.forename: Lian
authors.initials: L
authors.lastname: Ding
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guiying
authors.initials: G
authors.lastname: Chen
authors.forename: Chang
authors.initials: C
authors.lastname: Chen
authors.forename: Yi-Qiang
authors.initials: YQ
authors.lastname: Cheng
authors.forename: Hongliang
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D056572:Histone Deacetylase Inhibitors / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D056572:Histone Deacetylase Inhibitors