title: Protective effects of acacetin isolated from Ziziphora clinopodioides Lam. (Xintahua) on neonatal rat cardiomyocytes.
abstract: BACKGROUND
  The total flavonoids from ethanol extract of the aerial part of Ziziphora clinopodioides Lam. (Lamlaceae) (Xintahua) showed protective activities against rat acute myocardial ischemia in rats. This study aims to isolate acacetin, a flavonoid, from the aerial part of Z. clinopodioides, to develop an HPLC method for its detection, and to evaluate its protective effects on neonatal rat cardiomyocytes.
  
  
  METHODS
  Sephadex LH-20 silicagel and pillar layer chromatography silica gel were applied for the isolation and purification of acacetin and its structure was elucidated on the basis of (1)H and (13)C NMR spectroscopy. The content of acacetin in Z. clinopodioides collected from three different origins was determined by HPLC. The neonatal rat cardiomyocytes were isolated and cultured in vitro to establish a hypoxia/reoxygenation injury model. The viability of cardiomyocytes was measured by the MTT method. Changes of malondialdehyde (MDA) content in the medium were also determined.
  
  
  RESULTS
  The acacetin content in various batches of Z. clinopodioides ranged from 45.50 to 47.41 μg/g. Acacetin of 25, 10, 5 μg/mL significantly decreased the MDA content in a model of hypoxia/reoxygenation injury (P < 0.001, P < 0.001 and P = 0.033, respectively).
  
  
  CONCLUSIONS
  Acacetin protects neonatal cardiomyocytes from the damage induced by hypoxia/reoxygenation stress through reduction of lipid peroxidation and enhancement of the antioxidant activity.
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Chong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Zheng-Yi
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Institute of Metaria Medica, Zhejiang Academy of Medical Sciences, Hangzhou, 310013 China.
authors.forename: Xing-Yue
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Key Laboratory of Xinjiang Uighur Medicine, Xinjiang Institute of Materia Medica, Xinhua South Road 140, Urumqi, 830004 China.
authors.forename: Gui-Peng
authors.initials: GP
authors.lastname: Xue
keywords: Acacetin; HPLC; Hypoxia/reoxygenation; Neonatal rat cardiomyocytes; Ziziphora clinopodioides