title: [The expressions and meanings of BMP-7 and TGF-β in idiopathic pulmonary fibrosis and idiopathic nonspecific interstitial pneumonia].
abstract: OBJECTIVE
  To investigate the expressions of cytokines in idiopathic pulmonary fibrosis (IPF) and in idiopathic nonspecific interstitial pneumonia (INSIP); To discuss expressions and meanings of bone morphogenetic protein 7 (BMP-7) and transforming growth factor beta (TGF-β) in IPF and IPF.
  
  
  METHODS
  Selected 47 cases of idiopathic interstitial pneumonia (IIP), which were diagnosed by clinical-radiologic-pathologic (CRP), and classified into two groups which were group IPF (25 IPF) and group INSIP (22 INSIP, including 6 cellular pattern and 16 fibrosing pattern). The normal lung tissues were collected as the control group: The fresh tissues were made to detect more than 114 kinds of cytokines' expressions via Oligo GEArray gene microarray technology. Made a tissue microarray which applied EnVision immunohistochemistry technology to detect the expressions of BMP-7 and TGF-β in both kinds of IIPs. The two groups of patients were followed-up visited around 5 to 8 years and the survival curves were evaluated by Kaplan-Meier method.
  
  
  RESULTS
  According to gene microarray results, these two groups were up-expression in TGF family,IL family and TNF family. Most of BMP members were down-expression, in comparison with the control group, except BMP-5,BMP-8B and BMP-15. As the tissue microarray results demonstrated, compared with normal lung tissues,BMP-7 expressed decreasingly in IPF and INSIP groups (t1 = 27.618, P < 0.001; t2 = -12.404, P < 0.001). The expression of IPF were lower than INSIP (t = 5.387, P < 0.05); In INSIP group, patients of cellular pattern expressed BMP-7 more than fibrosing pattern's (t = -5.341, P < 0.001). There were dramatically increasing expressions of TGF-β in IPF and INSIP, when compared with the control group (t1 = 23.393, P < 0.001; t2 = -13.445, P < 0.001) and it presented negative correlation with BMP-7(group IPF: r = -0.771, P < 0.001; group INSIP: r = -0.729, P < 0.001). (3) Clinical follow-up data showed, the stability(improvement), deterioration and death rates of the group IPF and the group INSIP were, respectively, 0(0%), 2 (8%), 23 (92%) and 15 (68.1%), 3 (13.6%), 4 (18.2%). The results were statistically significant (all P < 0.05). The median survival time of the part with higher BMP-7 expression and the part with relatively lower BMP-7 expression, in the group IPF, were 110.8 and 66.4 months (t = -2.686, P < 0.05); In the group INSIP, were 146.4 and 74.9 months (t = -3.037, P < 0.05).
  
  
  CONCLUSIONS
  Cellular cytokines presented different expression profiles in IPF and INSIP patients. Differently with highly activated TGF-β, BMP-7 was inhibited in IIP patients, which would remind the degree of fibrosis and prognosis of IIP. BMP-7 would be expected to be a novel target for IIP pathogenesis and prognostic research.
authors.affiliation: DePartment of Pathology, Tongji HosPital of Tongji University, Shanghai 200065, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Gu
authors.forename: Benfang
authors.initials: B
authors.lastname: Luo
authors.affiliation: Email: yixhxf@163.com.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Yi
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhu
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Yu
authors.forename: Fei
authors.initials: F
authors.lastname: Han
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.forename: Xuyou
authors.initials: X
authors.lastname: Zhu
authors.forename: Weiwei
authors.initials: W
authors.lastname: Rui
authors.forename: Weizhe
authors.initials: W
authors.lastname: Qiu
authors.forename: Desheng
authors.initials: D
authors.lastname: Fan
mesh_terms: D055419:Bone Morphogenetic Protein 7 / Q000378:metabolism*; D006801:Humans; D054988:Idiopathic Interstitial Pneumonias / Q000378:metabolism*; D054990:Idiopathic Pulmonary Fibrosis / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D016212:Transforming Growth Factor beta