title: Expression and bioregulation of the kallikrein-related peptidases family in the human neutrophil.
abstract: The family of kallikrein-related peptidases (KLKs) has been identified in a variety of immunolabeled human tissue sections, but no previous study has experimentally confirmed their presence in the human neutrophil. We have investigated the expression and bioregulation of particular KLKs in the human neutrophil and, in addition, examined whether stimulation by a kinin B(1) receptor (B1R) agonist or fMet-Leu-Phe (fMLP) induces their secretion. Western blot analysis of neutrophil homogenates indicated that the MM of the KLKs ranged from 27 to 50 kDa. RT-PCR showed that blood neutrophils expressed only KLK1, KLK4, KLK10, KLK13, KLK14 and KLK15 mRNAs, whereas the non-differentiated HL-60 cells expressed most of them, with exception of KLK3 and KLK7. Nevertheless, mRNAs for KLK2, KLK5, KLK6 and KLK9 that were previously undetectable appeared after challenging with a mixture of cytokines. Both kinin B(1)R agonist and fMLP induced secretion of KLK1, KLK6, KLK10, KLK13 and KLK14 into the culture medium in similar amounts, whereas the B(1)R agonist caused the release of lower amounts of KLK2, KLK4 and KLK5. When secreted, the differing proteolytic activity of KLKs provides the human neutrophil with a multifunctional enzymatic capacity supporting a new dimension for its role in human disorders of diverse etiology.
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Alejandro J
authors.initials: AJ
authors.lastname: Lizama
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Yessica
authors.initials: Y
authors.lastname: Andrade
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Patricio
authors.initials: P
authors.lastname: Colivoro
authors.affiliation: Instituto de Fisiologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Jose
authors.initials: J
authors.lastname: Sarmiento
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Carola E
authors.initials: CE
authors.lastname: Matus
authors.affiliation: Instituto de Fisiologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Carlos B
authors.initials: CB
authors.lastname: Gonzalez
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Kanti D
authors.initials: KD
authors.lastname: Bhoola
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: Pamela
authors.initials: P
authors.lastname: Ehrenfeld
authors.affiliation: Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia, Universidad Austral de Chile, Valdivia, Chile cfiguero@uach.cl.
authors.forename: Carlos D
authors.initials: CD
authors.lastname: Figueroa
mesh_terms: D000328:Adult; D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007609:Kallidin / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D059748:Proteolysis / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D043783:Receptor, Bradykinin B1 / Q000819:agonists; D020840:Tissue Kallikreins / Q000235:genetics / Q000378:metabolism*; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D043783:Receptor, Bradykinin B1; D007609:Kallidin; D009240:N-Formylmethionine Leucyl-Phenylalanine; C052787:kallidin, des-Arg(10)-; D020840:Tissue Kallikreins
keywords: KLKs; Tissue kallikrein; bradykinin; kallikrein-related peptidases; kinin B1 receptor; neutrophils