title: [Prediction of the molecular response to pertubations from single cell measurements].
abstract: The response of protein signalization networks to perturbations is analysed from single cell measurements. This experimental approach allows characterizing the fluctuations in protein expression levels from cell to cell. The analysis is based on an information theoretic approach grounded in thermodynamics leading to a quantitative version of Le Chatelier principle which allows to predict the molecular response. Two systems are investigated: human macrophages subjected to lipopolysaccharide challenge, analogous to the immune response against Gram-negative bacteria and the response of the proteins involved in the mTOR signalizing network of GBM cancer cells to changes in partial oxygen pressure.
authors.affiliation: Université de Liège, département de chimie, chimie-physique théorique, B6c, allée de la Chimie, 3, B4000 Liège, Belgique.
authors.forename: Françoise
authors.initials: F
authors.lastname: Remacle
authors.affiliation: Crump institute for molecular imaging, department of molecular and medical pharmacology, university of California, department of chemistry and biochemistry, David Geffen school of medicine, Los Angeles, CA 90095, États-Unis.
authors.forename: Raphael D
authors.initials: RD
authors.lastname: Levine
mesh_terms: D000818:Animals; D002450:Cell Communication; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002468:Cell Physiological Phenomena; D005909:Glioblastoma; D006090:Gram-Negative Bacteria / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology; D015398:Signal Transduction / Q000502:physiology*; D058570:TOR Serine-Threonine Kinases / Q000502:physiology; D013816:Thermodynamics
chemical_list: D008070:Lipopolysaccharides; D058570:TOR Serine-Threonine Kinases