title: A staggered decameric assembly of human C-reactive protein stabilized by zinc ions revealed by X-ray crystallography.
abstract: Human C-reactive protein (CRP) is an acute phase protein, which harbours both host defence and scavenging properties. In this study, we obtained two new crystal forms of CRP, where CRP forms a symmetric, staggered dimer of pentamers. In one of these structures, obtained in the presence of HIV-1 Tat protein, this dimer of pentamers is stabilized by two zinc ions trapped within a cleft of the effector face of CRP. These two decameric interfaces involve complementary surfaces of CRP pentamers and bury a large area of ~2000 Å(2) per pentamer, suggesting a biological role of this interface. These two novel decameric interfaces and the involvement of zinc might have important consequences in the understanding of CRP biological functions.
authors.forename: Christophe
authors.initials: C
authors.lastname: Guillon
authors.forename: Ulrick Mavoungou
authors.initials: UM
authors.lastname: Bigouagou
authors.forename: Christelle
authors.initials: C
authors.lastname: Folio
authors.forename: Pascale
authors.initials: P
authors.lastname: Jeannin
authors.forename: Yves
authors.initials: Y
authors.lastname: Delneste
authors.affiliation: "Biocrystallography and Structural Biology of Therapeutic Targets", UMR 5086 CNRS Universite de Lyon, IBCP 7, passage du Vercors, 69367 Lyon cedex 7, France. christophe.guillon@ibcp.fr.
authors.forename: Patrice
authors.initials: P
authors.lastname: Gouet
mesh_terms: D001665:Binding Sites; D002097:C-Reactive Protein / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray / Q000379:methods*; D006801:Humans; D007477:Ions / Q000737:chemistry; D008958:Models, Molecular; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D055503:Protein Multimerization; D055550:Protein Stability; D015032:Zinc / Q000737:chemistry*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*; D000099153:Pentraxins
chemical_list: D002097:C-Reactive Protein; D007477:Ions; D009419:Nerve Tissue Proteins; D015032:Zinc; D054322:tat Gene Products, Human Immunodeficiency Virus; D000099153:Pentraxins