title: MiRNA-125a-5p inhibits glioblastoma cell proliferation and promotes cell differentiation by targeting TAZ.
abstract: Glioblastoma (GBM) is the most lethal brain tumor due to the resistance to conventional therapies, such as radiotherapy and chemotherapy. TAZ, an important mediator of the Hippo pathway, was found to be up-regulated in diverse cancers, including in GBM, and plays important roles in tumor initiation and progression. However, little is known about the regulation of TAZ expression in tumors. In this study, we found that miR-125a-5p is an important regulator of TAZ in glioma cells by directly targeting the TAZ 3' UTR. MiR-125a-5p levels are inversely correlated with that of TAZ in normal astrocytes and a panel of glioma cell lines. MiR-125a-5p represses the expression of TAZ target genes, including CTGF and survivin, and inhibits cell proliferation and induces the differentiation of GBM cells; whereas over-expression of TAZ rescues the effects of miR-125a-5p. This study revealed a mechanism for TAZ deregulation in glioma cells, and also demonstrated a tumor suppressor role of miR-125a-5p in glioblastoma cells.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China.
authors.forename: Gelei
authors.initials: G
authors.lastname: Xiao
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Peng
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China.
authors.forename: Dingyang
authors.initials: D
authors.lastname: Liu
authors.affiliation: Cancer Research Institute, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Zeyou
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Liao
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China; Cancer Research Institute, Central South University, Changsha, Hunan 410008, PR China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, PR China; The Institute of Skull Base Surgery & Neuro-oncology at Hunan, Changsha, Hunan 410008, PR China. Electronic address: xry69@163.com.
authors.forename: Xianrui
authors.initials: X
authors.lastname: Yuan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D002454:Cell Differentiation / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D052138:Genes, Neoplasm; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators; D014157:Transcription Factors; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins
chemical_list: D020413:3' Untranslated Regions; D047908:Intracellular Signaling Peptides and Proteins; C531888:MIRN125 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; D000091364:Transcriptional Coactivator with PDZ-Binding Motif Proteins; C551991:WWTR1 protein, human
keywords: Cell differentiation; Glioblastoma; TAZ; miR-125a-5p