title: Reduction of HIV-1 infectivity through endoplasmic reticulum-associated degradation-mediated Env depletion.
abstract: During the HIV-1 replicative cycle, the gp160 envelope is processed in the secretory pathway to mature into the gp41 and gp120 subunits. Misfolded proteins located within the endoplasmic reticulum (ER) are proteasomally degraded through the ER-associated degradation (ERAD) pathway, a quality control system operating in this compartment. Here, we exploited the ERAD pathway to induce the degradation of gp160 during viral production, thus leading to the release of gp120-depleted viral particles.
authors.affiliation: Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy anna.cereseto@unitn.it antonio.casini@unitn.it.
authors.forename: Antonio
authors.initials: A
authors.lastname: Casini
authors.affiliation: Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Olivieri
authors.affiliation: Nanobiotechnology Laboratory, Federal University of Uberlandia, Uberlandia, MG, Brazil.
authors.forename: Lara
authors.initials: L
authors.lastname: Vecchi
authors.affiliation: International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
authors.forename: Oscar R
authors.initials: OR
authors.lastname: Burrone
authors.affiliation: Centre for Integrative Biology (CIBIO), University of Trento, Trento, Italy anna.cereseto@unitn.it antonio.casini@unitn.it.
authors.forename: Anna
authors.initials: A
authors.lastname: Cereseto
mesh_terms: D002460:Cell Line; D060746:Endoplasmic Reticulum-Associated Degradation; D019302:HIV Envelope Protein gp160 / Q000378:metabolism*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D059748:Proteolysis
chemical_list: D019302:HIV Envelope Protein gp160; C514797:gp160 protein, Human immunodeficiency virus 1