title: Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model.
abstract: OBJECTIVE
  To investigate the effect of ozone and/or taurine treatment comparatively on testicular damage due to ischemia/ reperfusion (I/R) injury in an experimental torsion model in rats.
  
  
  METHODS
  Adult Wistar rats with and without torsion/detorsion were used. In order to monitor the effect of ozone and/or taurine treatment on testicular damage due to I/R injury, following histopathological investigation apoptotic indexes were scored by TUNEL method. Moreover, tumor necrosis factor receptor 1 (TNFR1), caspase 3, caspase 8, endothelial nitric oxide synthase (eNOS), tumor necrosis factor alpha (TNFα), and cytochrome C immunostainings were performed and the levels of malondialdehyde, glutathione peroxidase, superoxide dismutase, catalase, total sulfhydryl, and nitric oxide were determined in the testicular tissue.
  
  
  RESULTS
  Intraperitoneal ozone and/or taurine treatment prevented both histopathological damage and increase in the apoptotic index. Torsion did not exert an effect on the levels of TNFα and cytochrome C. Ozone and/or taurine treatment prevented increases in TNFR1, caspase 3, and caspase 8. The level of oxidative stress markers was unchanged. The increases in NO level and eNOS expression were prevented by ozone and/or taurine treatment in I/R groups.
  
  
  CONCLUSIONS
  Using ozone therapy and/or taurine before reperfusion may be a solution for germ cell degeneration resulting from testicular torsion and related infertility.
authors.forename: Tolga Reşat
authors.initials: TR
authors.lastname: Aydos
authors.forename: Mehmet Murad
authors.initials: MM
authors.lastname: Başar
authors.forename: Oğuz
authors.initials: O
authors.lastname: Kul
authors.forename: Hasan Tarık
authors.initials: HT
authors.lastname: Atmaca
authors.forename: Tuba
authors.initials: T
authors.lastname: Uzunalıoğlu
authors.forename: Üçler
authors.initials: Ü
authors.lastname: Kisa
authors.forename: Oğuzhan Ekin
authors.initials: OE
authors.lastname: Efe
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D004195:Disease Models, Animal; D020287:In Situ Nick-End Labeling; D008297:Male; D010083:Oxidants, Photochemical / Q000627:therapeutic use*; D010126:Ozone / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000032:analysis; D015427:Reperfusion Injury / Q000188:drug therapy*; D013086:Spermatic Cord Torsion; D013654:Taurine / Q000627:therapeutic use*; D013737:Testis / Q000098:blood supply* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010083:Oxidants, Photochemical; D047888:Receptors, Tumor Necrosis Factor, Type I; D013654:Taurine; D010126:Ozone