title: Silk fibroin/gelatin electrospun nanofibrous dressing functionalized with astragaloside IV induces healing and anti-scar effects on burn wound.
abstract: Functional wound dressing has provided new challenges for researchers who focus on burn to improve skin graft quality, reduce scarring, and develop a pluristratified dermal or epidermal construct of a burn wound. This study aimed to investigate the effect of a silk fibroin/gelatin (SF/GT) electrospun nanofibrous dressing loaded with astragaloside IV (AS) on deep partial-thickness burn wound. AS-loaded SF/GT-blended nanofibrous dressing was prepared by electrospinning nanotechnology. The optimal ratio (25:75) of silk fibroin to gelatin was further optimized by evaluating ATR-FTIR characteristics, mechanical properties, porosity, swelling rate, degradation, and release profile of the AS-loaded SF/GT nanofibrous dressing. In contrast to the blank control, the AS-loaded SF/GT nanofibrous dressing promoted cell adhesion and proliferation with good biocompatibility in vitro (p<0.01). This dressing also accelerated wound healing and inhibited scar formation in vivo by stimulating wound closure (p<0.05), increasing angiogenesis, regulating newly formed types of collagen, and improving collagen organization. These results showed that SF/GT nanofibrous dressing is a promising topical drug delivery system. Furthermore, AS-functionalized SF/GT nanofibrous dressing is an excellent topical therapeutic that could be applied to promote healing and elicit anti-scar effects on partial-thickness burn wound.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Shan
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Peng
authors.affiliation: College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China; Jiangsu Engineering Research Center for New-Type External and Transdermal Preparations, PR China. Electronic address: gaojianqing@zju.edu.cn.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D001458:Bandages; D002056:Burns / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D002448:Cell Adhesion; D002921:Cicatrix / Q000209:etiology / Q000517:prevention & control*; D003094:Collagen / Q000378:metabolism; D016503:Drug Delivery Systems; D005349:Fibroins / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D057139:Nanofibers; D018919:Neovascularization, Physiologic / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage* / Q000494:pharmacology; D047011:Silk / Q000737:chemistry; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014315:Triterpenes / Q000008:administration & dosage* / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D012503:Saponins; D047011:Silk; D014315:Triterpenes; C052064:astragaloside A; D005780:Gelatin; D003094:Collagen; D005349:Fibroins
keywords: Astragaloside IV; Burn; Nanofibrous dressing; Scar inhibition; Wound healing