title: Effects of tityustoxin on cerebral inflammatory response in young rats.
abstract: Accidents caused by scorpion stings, mainly affecting children, are considered an important cause of morbidity and mortality in tropical countries. Clinical studies demonstrate the relevant role of systemic inflammatory events in scorpion envenoming. However, remains poorly understood whether the major lethal component in Tityus serrulatus venom, tityustoxin (TsTX), is able to induce inflammatory responses in the cerebral microcirculation. In this study, we systematically examined leukocyte recruitment into the CNS in response to TsTX injection. Accordingly, developing rats were subjected to a subcutaneous (s.c.) injection of TsTX (0.75mg/kg), and leukocyte recruitment (i.e., 4, 8 and 12h after injection) and TNF-α levels were evaluated. Rats injected with TsTX presented a significant increase in leukocyte rolling and adhesion and higher levels of TNF-α at all time points studied, compared to the control group. Altogether, this work demonstrates the triggering of neuroimmunological mechanisms induced by TsTX injection in young rats.
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: iva_tereza@yahoo.com.br.
authors.forename: Iva Tereza
authors.initials: IT
authors.lastname: Van Fraga
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: marcelolf@uol.com.br.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Limborço-Filho
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: onesiacristina@yahoo.com.br.
authors.forename: Onésia Cristina Oliveira
authors.initials: OC
authors.lastname: Lima
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: norinneq@yahoo.com.br.
authors.forename: Norinne
authors.initials: N
authors.lastname: Lacerda-Queiroz
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil; Centro de Tecnologia e Imagem em Magneto-Ressonância (CTPMag), Escola de Engenharia, UFMG, Brazil. Electronic address: maiaguidine@gmail.com.
authors.forename: Patrícia Alves Maia
authors.initials: PA
authors.lastname: Guidine
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil; Centro de Tecnologia e Imagem em Magneto-Ressonância (CTPMag), Escola de Engenharia, UFMG, Brazil. Electronic address: mfdm@icb.ufmg.br.
authors.forename: Márcio Flávio Dutra
authors.initials: MF
authors.lastname: Moraes
authors.affiliation: Laboratório de Fisiologia de Insetos Hematófagos, Departamento de Parasitologia, ICB, UFMG, Brazil. Electronic address: rnaraujo@icb.ufmg.br.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Nascimento Araújo
authors.affiliation: Laboratório de Nutrição Experimental (LNNE), Faculdade de Farmácia, UFMG, Brazil; Centro de Tecnologia e Imagem em Magneto-Ressonância (CTPMag), Escola de Engenharia, UFMG, Brazil. Electronic address: tmoraes@farmacia.ufmg.br.
authors.forename: Tasso
authors.initials: T
authors.lastname: Moraes-Santos
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: massensini@gmail.com.
authors.forename: André Ricardo
authors.initials: AR
authors.lastname: Massensini
authors.affiliation: Laboratório de Neuroimunopatologia Experimental (NIPE), Departamento de Patologia, ICB, UFMG, Brazil. Electronic address: rosa@icb.ufmg.br.
authors.forename: Rosa Maria Esteves
authors.initials: RM
authors.lastname: Arantes
authors.affiliation: Núcleo de Neurociências (NNC), Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas (ICB), Universidade Federal de Minas Gerais (UFMG), MG, Brazil. Electronic address: julianat@icb.ufmg.br.
authors.forename: Juliana
authors.initials: J
authors.lastname: Carvalho-Tavares
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D002448:Cell Adhesion; D004660:Encephalitis / Q000209:etiology / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000502:physiology; D008297:Male; D055806:Microvessels / Q000502:physiology; D009498:Neurotoxins / Q000378:metabolism / Q000633:toxicity*; D017208:Rats, Wistar; D065008:Scorpion Stings / Q000209:etiology / Q000276:immunology / Q000378:metabolism; D012604:Scorpion Venoms / Q000378:metabolism / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D009498:Neurotoxins; D012604:Scorpion Venoms; D014409:Tumor Necrosis Factor-alpha; C003609:tityustoxin
keywords: Brain inflammation; Leukocyte trafficking; Microcirculation; Scorpion envenoming; TsTX