title: Whole blood measurement of histone modifications linked to the epigenetic regulation of gene expression.
abstract: Rapid progress is being made to understand the regulatory mechanisms that underlie the epigenetic control of gene expression through histone modification. It is now recognized that this plays a major role in normal development and disease. This unit describes the application of flow cytometry to the study of epigenetic mechanisms by combining labeling of individual histone modifications and phenotypic markers, and it also discusses practical issues to optimize staining. The focus is on normal blood and samples from leukemia patients, but it can also be applied to cells grown in tissue culture.
authors.affiliation: Ontario Cancer Institute/Princess Margaret Cancer Centre, Toronto, Ontario, Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Watson
authors.affiliation: Ontario Cancer Institute/Princess Margaret Cancer Centre, Toronto, Ontario, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Hedley
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D001769:Blood / Q000378:metabolism*; D044127:Epigenesis, Genetic; D005434:Flow Cytometry / Q000379:methods*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D016707:Tissue Fixation
chemical_list: D015415:Biomarkers; D006657:Histones
keywords: drug treatment; epigenetics; histone; histone modifications; leukemia