title: Doped zinc sulfide quantum dots based phosphorescence turn-off/on probe for detecting histidine in biological fluid.
abstract: We report a turn-on phosphorescence probe for detection of histidine based on Co(2+)-adsorbed N-acetyl-L-cysteine (NAC) capped Mn: ZnS quantum dots (QDs) which is directly synthesized by the hydrothermal method. The phosphorescence of NAC-Mn: ZnS QDs is effectively quenched by Co(2+) attributing to the adsorption of Co(2+) onto the surface of QDs with a concomitant in suppressing the recombination process of hole and electron of QDs. The phosphorescence of Co(2+)-adsorbed NAC-Mn: ZnS QDs can be recovered by binding of Co(2+) with histidine. The quenching and regeneration of the phosphorescence of NAC-Mn: ZnS QDs have been studied in detail. The as-prepared QDs-based probe is applied to determine histidine with a linear range of 1.25-30 μM and a detection limit of 0.74 μM. The relative standard deviation for eleven repeat detections of 20 μM histidine is 0.65%. Co(2+)-adsorbed NAC-Mn: ZnS QDs show high sensitivity and good selectivity to histidine over other amino acids, metal ions and co-existing substances. The proposed QDs probe has been successfully applied to determination of histidine in human urine samples with good recoveries of 98.5-103%.
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China; School of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Bian
authors.affiliation: School of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Wei
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Dong
authors.affiliation: School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. Electronic address: smshuang@sxu.edu.cn.
authors.forename: Shaomin
authors.initials: S
authors.lastname: Shuang
authors.affiliation: Partner State Key Laboratory of Environmental and Biological Analysis, and Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, China. Electronic address: mmfchoi@gmail.com.
authors.forename: Martin M F
authors.initials: MM
authors.lastname: Choi
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry*; D000327:Adsorption; D000328:Adult; D003035:Cobalt / Q000737:chemistry; D006639:Histidine / Q000737:chemistry / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008345:Manganese / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*; D017967:Zinc Compounds / Q000737:chemistry*
chemical_list: D049408:Luminescent Agents; D013440:Sulfides; D017967:Zinc Compounds; D003035:Cobalt; D008345:Manganese; D006639:Histidine; C031238:zinc sulfide; D000111:Acetylcysteine
keywords: Biological fluids; Doped quantum dots; Histidine; Phosphorescence probe