title: Intracellular distribution of 3,6-bis(3-alkylguanidino)acridines determines their cytotoxicity.
abstract: Cytotoxicity of two derivatives of 3,6-bis(3-alkylguanidino)acridines (GNDAs; pentyl- and hexyl-GNDA) was determined against three cell lines: a murine immortalized fibroblast cell line NIH-3T3, a human ovarian carcinoma cell line A2780, and a human neuroblastoma cell line SH-SY5Y. We found out that these GNDAs were cytotoxic against A2780 and NIH-3T3 cells but they showed only a marginal cytotoxicity against neuroblastoma cells SH-SY5Y. To explain differences in cytotoxicity, intracellular distribution of GNDAs was monitored. GNDAs were accumulated in A2780 and NIH-3T3 cells in the nuclei (fluorescence microscopy). In contrast to these cell lines, in SH-SY5Y cells, GNDAs were localized outside of the nuclei, at the plasma membrane and surroundings, extending also to the cytosol. This distribution of GNDAs was confirmed by an ImageStream Flow Cytometer. Acetylcholinesterase (AChE) activity in the SH-SY5Y cells decreased upon incubation with GNDAs. Kinetic studies showed that GNDAs were able to inhibit AChE by the same mode as tacrine (9-amino-1,2,3,4-tetrahydroacridine), a known inhibitor of AChE. A low cytotocity of GNDAs against SH-SY5Y cells could be caused by their affinity to AChE (the enzyme is localized mainly at the plasma membrane). The interaction of GNDAs with AChE may affect their intracellular distribution and consequently the cytotoxicity.
  
  
  BACKGROUND
  acetylcholinesterase, acridine, neuroblastoma cell line SH-SY5Y.
authors.forename: L
authors.initials: L
authors.lastname: Krajnakova
authors.forename: H
authors.initials: H
authors.lastname: Paulikova
authors.forename: Z
authors.initials: Z
authors.lastname: Bacova
authors.forename: J
authors.initials: J
authors.lastname: Bakos
authors.forename: L
authors.initials: L
authors.lastname: Janovec
authors.forename: J
authors.initials: J
authors.lastname: Imrich
authors.forename: L
authors.initials: L
authors.lastname: Hunakova