title: Comparative analysis of cardiovascular development related genes in stem cells isolated from deciduous pulp and adipose tissue.
abstract: Human exfoliated deciduous teeth (SHED) and adipose stem cells (ASC) were suggested as alternative cell choice for cardiac regeneration. However, the true functionability of these cells toward cardiac regeneration is yet to be discovered. Hence, this study was carried out to investigate the innate biological properties of these cell sources toward cardiac regeneration. Both cells exhibited indistinguishable MSCs characteristics. Human stem cell transcription factor arrays were used to screen expression levels in SHED and ASC. Upregulated expression of transcription factor (TF) genes was detected in both sources. An almost equal percentage of >2-fold changes were observed. These TF genes fall under several cardiovascular categories with higher expressions which were observed in growth and development of blood vessel, angiogenesis, and vasculogenesis categories. Further induction into cardiomyocyte revealed ASC to express more significantly cardiomyocyte specific markers compared to SHED during the differentiation course evidenced by morphology and gene expression profile. Despite this, spontaneous cellular beating was not detected in both cell lines. Taken together, our data suggest that despite being defined as MSCs, both ASC and SHED behave differently when they were cultured in a same cardiomyocytes culture condition. Hence, vigorous characterization is needed before introducing any cell for treating targeted diseases.
authors.affiliation: Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Zhang Xin
authors.initials: ZX
authors.lastname: Loo
authors.affiliation: Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wijenthiran
authors.identifier: 0000-0001-9685-2512
authors.initials: W
authors.lastname: Kunasekaran
authors.affiliation: Hygieia Innovation Sdn. Bhd (852106-M), Lot 1G-2G, Lanai Complex No. 2, Persiaran Seri Perdana, Precint 10, 62250 Putrajaya, Malaysia.
authors.forename: Vijayendran
authors.initials: V
authors.lastname: Govindasamy
authors.affiliation: Department of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Sabri
authors.identifier: 0000-0001-9510-1864
authors.initials: S
authors.lastname: Musa
authors.affiliation: Departments of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Noor Hayaty
authors.initials: NH
authors.lastname: Abu Kasim
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002319:Cardiovascular System / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D048430:Cell Shape; D002648:Child; D002675:Child, Preschool; D003782:Dental Pulp / Q000166:cytology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D050437:Genes, Developmental; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism; D014094:Tooth, Deciduous / Q000166:cytology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D014157:Transcription Factors