title: Assessment of immunogenicity and protective efficacy of Microsporum canis secreted components coupled to monophosphoryl lipid-A adjuvant in a vaccine study using guinea pigs.
abstract: Microsporum canis is the most common dermatophyte in pets and is of zoonotic importance but currently there is no effective vaccine available to prevent dermatophytosis. The aim of this work was to assess the immunogenicity and protective efficacy of secreted components (SC) from M. canis adjuvanted with the monophosphoryl lipid-A (MPLA), in a vaccine study using the guinea pig as an experimental model. Animals were vaccinated with either the SC adjuvanted with the MPLA, the MPLA adjuvant alone or PBS three times at two-week intervals, until 42 days prior to M. canis infection. A blind evaluation of dermatophytosis symptoms development and fungal persistence in skin was monitored weekly. The antibody response towards the SC and the levels of Interferon (IFN)γ and Interleukin-4 expressed in peripheral blood mononuclear cells were assessed along or at the end of the study period respectively. The animals that received MPLA had a significantly lower clinical score than those inoculated with PBS. However, no significant difference was observed between the guinea pigs vaccinated with the SC adjuvanted with the MPLA and those having received MPLA alone. The results also showed that vaccination induced a strong antibody response towards the SC and an increase in IFNγ mRNA level. Our results show that the MPLA adjuvant used in this vaccine study can induce per se a partial protection against a M. canis infection. Although they induce a delayed-type hypersensitivity reaction in guinea pigs, the SC do not confer a protection under the present experimental conditions.
authors.affiliation: Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Cambier
authors.affiliation: Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium; Parasitology and Parasitic Diseases Unit, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania.
authors.forename: Elena-Tatiana
authors.initials: ET
authors.lastname: Băguţ
authors.affiliation: Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Heinen
authors.affiliation: Laboratory of Venin and Biological Activities, Jean-Francois Champollion University Center, Albi, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Tabart
authors.affiliation: FARAH, Department of Morphology and Pathology, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Nadine
authors.initials: N
authors.lastname: Antoine
authors.affiliation: Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium. Electronic address: bmignon@ulg.ac.be.
authors.forename: Bernard
authors.initials: B
authors.lastname: Mignon
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D003883:Arthrodermataceae; D003881:Dermatomycoses / Q000517:prevention & control / Q000662:veterinary; D005657:Fungal Vaccines / Q000276:immunology*; D006168:Guinea Pigs; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008050:Lipid A / Q000031:analogs & derivatives* / Q000737:chemistry; D008864:Microsporum / Q000276:immunology*; D014611:Vaccination
chemical_list: D000276:Adjuvants, Immunologic; D005657:Fungal Vaccines; D008050:Lipid A; C048436:monophosphoryl lipid A
keywords: Dermatophytes; Microsporum canis; Monophosphoryl lipid-A adjuvant; Vaccination