title: The Milieu Intérieur study - an integrative approach for study of human immunological variance.
abstract: The Milieu Intérieur Consortium has established a 1000-person healthy population-based study (stratified according to sex and age), creating an unparalleled opportunity for assessing the determinants of human immunologic variance. Herein, we define the criteria utilized for participant enrollment, and highlight the key data that were collected for correlative studies. In this report, we analyzed biological correlates of sex, age, smoking-habits, metabolic score and CMV infection. We characterized and identified unique risk factors among healthy donors, as compared to studies that have focused on the general population or disease cohorts. Finally, we highlight sex-bias in the thresholds used for metabolic score determination and recommend a deeper examination of current guidelines. In sum, our clinical design, standardized sample collection strategies, and epidemiological data analyses have established the foundation for defining variability within human immune responses.
authors.affiliation: Center for Human Immunology, Institut Pasteur, Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, Paris, France; INSERM U818, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Thomas
authors.affiliation: Center for Human Immunology, Institut Pasteur, Paris, France; Center for Bioinformatics, Institut Pasteur, Paris, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Rouilly
authors.affiliation: Laboratory of Human Evolutionary Genetics, Department of Genomes & Genetics, Institut Pasteur, Paris, France; CNRS URA3012, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Patin
authors.affiliation: Center for Human Immunology, Institut Pasteur, Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, Paris, France; INSERM U818, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Alanio
authors.affiliation: PIRC, Institut Pasteur, Paris, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Dubois
authors.affiliation: PIRC, Institut Pasteur, Paris, France.
authors.forename: Cécile
authors.initials: C
authors.lastname: Delval
authors.affiliation: Biotrial, Rennes, France.
authors.forename: Louis-Guillaume
authors.initials: LG
authors.lastname: Marquier
authors.affiliation: Biotrial, Rennes, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fauchoux
authors.affiliation: Biotrial, Rennes, France.
authors.forename: Seloua
authors.initials: S
authors.lastname: Sayegrih
authors.affiliation: Unit of Emerging Diseases Epidemiology, Institut Pasteur, Paris, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Vray
authors.affiliation: Center for Human Immunology, Institut Pasteur, Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, Paris, France; INSERM U818, France.
authors.forename: Darragh
authors.initials: D
authors.lastname: Duffy
authors.affiliation: Laboratory of Human Evolutionary Genetics, Department of Genomes & Genetics, Institut Pasteur, Paris, France; CNRS URA3012, France. Electronic address: quintana@pasteur.fr.
authors.forename: Lluis
authors.initials: L
authors.lastname: Quintana-Murci
authors.affiliation: Center for Human Immunology, Institut Pasteur, Paris, France; Laboratory of Dendritic Cell Immunobiology, Department of Immunology, Institut Pasteur, Paris, France; INSERM U818, France; INSERM UMS20, France. Electronic address: albertm@pasteur.fr.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Albert
mesh_terms: D000328:Adult; D000367:Age Factors; D000368:Aged; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D003586:Cytomegalovirus Infections / Q000276:immunology*; D005260:Female; D005602:France; D006801:Humans; D007107:Immune System / Q000276:immunology*; D008297:Male; D024821:Metabolic Syndrome / Q000276:immunology*; D008875:Middle Aged; D012307:Risk Factors; D012737:Sex Factors; D012907:Smoking / Q000276:immunology*; D012959:Socioeconomic Factors; D055815:Young Adult
keywords: Baseline serologic data; CMV; Cohort design; Healthy donor; Immune phenotypes; Metabolic syndrome