title: Up-regulation of TIMP-2 expression promotes SHI-1 leukemic cells proliferation and infiltration in immunodeficiency mice.
abstract: BACKGROUND
  MMPs and TIMPs play important roles in tumor angiogenesis and invasion. Studies have shown that TIMP-2 has two roles in tumor invasion. However, its role in leukemic infiltration has not been well investigated. This study explored the roles of TIMP-2 in extramedullary infiltration of acute monocytic leukemic SHI-1 cells both in vitro and in vitro.
  
  
  METHODS
  A retroviral vector carrying the human TIMP-2 cDNA was constructed and transfected into the monocytic leukemic cell line SHI-1. The expression of TIMP-2 in the positive clones was determined. The proliferation of SHI-1 cells was examined by MTT assay. Trans-Matrigel invasion assays were used to investigate the infiltration ability in vitro. SHI-1 cells were intravenously injected into pre-treated nu/nu mice to investigate the infiltration ability feature in vitro.
  
  
  RESULTS
  The expression of TIMP-2 on the cell membrane was significantly elevated in SHI-1/TIMP-2 cells. Over-expression of TIMP-2 promoted the cells proliferation and the invasions in vitro. The SHI-1/TIMP-2 cells demonstrated higher infiltration ability when intravenously injected into nu/nu mice.
  
  
  CONCLUSIONS
  Over-expression of TIMP-2, especially on the cell membrane, may play important roles in promoting the proliferation and infiltration of SHI-1 leukemic cells.
authors.affiliation: Department of Hematology, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.
authors.forename: Zhenjiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215600, China. Email: szchenzx@263.net.
authors.forename: Zixing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215600, China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Cen
authors.affiliation: Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215600, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215600, China.
authors.forename: Qiaocheng
authors.initials: Q
authors.lastname: Qiu
authors.affiliation: Leukemia Research Unit, Jiangsu Institute of Hematology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215600, China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000502:physiology; D006801:Humans; D017254:Leukemic Infiltration / Q000503:physiopathology*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D019716:Tissue Inhibitor of Metalloproteinase-2