title: Sampling phasic dopamine signaling with fast-scan cyclic voltammetry in awake, behaving rats.
abstract: Fast-scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the in vivo measurement of extracellular fluctuations in multiple chemical species. The technique is frequently utilized to sample sub-second (phasic) concentration changes of the neurotransmitter dopamine in awake and behaving rats. Phasic dopamine signaling is implicated in reinforcement, goal-directed behavior, and locomotion, and FSCV has been used to investigate how rapid changes in striatal dopamine concentration contribute to these and other behaviors. This unit describes the instrumentation and construction, implantation, and use of components required to sample and analyze dopamine concentration changes in awake rats with FSCV.
authors.affiliation: Graduate Program in Neuroscience, University of Illinois at Chicago, Chicago, Illinois.
authors.forename: S M
authors.initials: SM
authors.lastname: Fortin
authors.affiliation: Graduate Program in Neuroscience, University of Illinois at Chicago, Chicago, Illinois.
authors.forename: J J
authors.initials: JJ
authors.lastname: Cone
authors.affiliation: Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle, Washington.
authors.forename: S
authors.initials: S
authors.lastname: Ng-Evans
authors.affiliation: Department of Cell Physiology & Pharmacology, University of Leicester, Leicester, United Kingdom.
authors.forename: J E
authors.initials: JE
authors.lastname: McCutcheon
authors.affiliation: Graduate Program in Neuroscience, University of Illinois at Chicago, Chicago, Illinois.|Department of Psychology, University of Illinois at Chicago, Chicago, Illinois.
authors.forename: M F
authors.initials: MF
authors.lastname: Roitman
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*; D014851:Wakefulness / Q000502:physiology*
chemical_list: D004298:Dopamine
keywords: dopamine; fast-scan cyclic voltammetry; motivation; nucleus accumbens; reinforcement; reward