title: Synthesis and anti-Trypanosoma cruzi activity of diaryldiazepines.
abstract: Chagas disease is a so-called "neglected disease" and endemic to Latin America. Nifurtimox and benznidazole are drugs that have considerable efficacy in the treatment of the acute phase of the disease but cause many significant side effects. Furthermore, in the Chronic Phase its efficiency is reduced and their therapeutic effectiveness is dependent on the type of T. cruzi strain. For this reason, the present work aims to drive basic research towards the discovery of new chemical entities to treat Chagas disease. Differently substituted 5,7-diaryl-2,3-dihydro-1,4-diazepines were synthesized by cyclocondensation of substituted flavones with ethylenediamine and tested as anti-Trypanosoma cruzi candidates. Epimastigotes of the Y strain from T. cruzi were used in this study and the number of parasites was determined in a Neubauer chamber. The most potent diaryldiazepine that reduced epimastigote proliferation exhibited an IC50 value of 0.25 μM, which is significantly more active than benznidazole.
authors.affiliation: Departamento de Química, ICEB, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, CEP, Ouro Preto, MG 35400-000, Brazil. jc_menezes2@hotmail.com.
authors.forename: Júlio César L
authors.initials: JC
authors.lastname: Menezes
authors.affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Brazil. luanavazz@yahoo.com.br.
authors.forename: Luana Beatriz A
authors.initials: LB
authors.lastname: Vaz
authors.affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Brazil. paulinhamav@gmail.com.
authors.forename: Paula Melo
authors.initials: PM
authors.lastname: de Abreu Vieira
authors.affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Brazil. katia.fonseca@gmail.com.
authors.forename: Kátia
authors.initials: K
authors.lastname: da Silva Fonseca
authors.affiliation: Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas, ICEB II, Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto 35400-000, Brazil. claudiamartinscarneiro@gmail.com.
authors.forename: Cláudia Martins
authors.initials: CM
authors.lastname: Carneiro
authors.affiliation: Departamento de Química, ICEB, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, CEP, Ouro Preto, MG 35400-000, Brazil. jason@iceb.ufop.br.
authors.forename: Jason G
authors.initials: JG
authors.lastname: Taylor
mesh_terms: D000818:Animals; D001381:Azepines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D014344:Trypanocidal Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D001381:Azepines; D014344:Trypanocidal Agents