title: Preservation of Capillary-beds in Rat Lung Tissue Using Optimized Chemical Decellularization.
abstract: Promoting regeneration using scaffolds created by decellularizing native tissue is becoming a popular technique applied to a variety of tissues. We demonstrate a method to decellularize highly vascular tissue keeping the vascular structure intact down to the capillary scale. Using vascular corrosion casting (VCC), we created a method for quantitatively assessing the functionality of vascular extracellular matrix (ECM) following decellularization. Murine lung tissue was decellularized using a number of techniques, then characterized using standard histological methods, as well as our quantitative VCC (qVCC) technique. Using an optimized acellular method, we successfully decellularized lung tissue while leaving behind a patent vascular network based on qualitative and quantitative histological methods.
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Nagao
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Ouyang
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America.
authors.forename: Renee
authors.initials: R
authors.lastname: Keller
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America.
authors.forename: Curtis
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America.
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Suggs
authors.affiliation: Department of Biomedical Engineering, The University of Texas at Austin, 107 West Dean Keeton Street, Austin, Texas, TX 78712, United States of America ; J. Crayton Pruitt Family Department of Biomedical Engineering, The University of Florida, Gainesville, FL 32611, United States of America.
authors.forename: Christine E
authors.initials: CE
authors.lastname: Schmidt