title: Normal adult survival but reduced Bemisia tabaci oviposition rate on tomato lines carrying an introgression from S. habrochaites.
abstract: BACKGROUND
  Host plant resistance has been proposed as one of the most promising approaches in whitefly management. Already in 1995 two quantitative trait loci (Tv-1 and Tv-2) originating from S. habrochaites CGN1.1561 were identified that reduced the oviposition rate of the greenhouse whitefly (Trialeurodes vaporariorum). After this first study, several others identified QTLs affecting whitefly biology as well. Generally, the QTLs affecting oviposition were highly correlated with a reduction in whitefly survival and the presence of high densities of glandular trichomes type IV. The aim of our study was to further characterize Tv-1 and Tv-2, and to determine their role in resistance against Bemisia tabaci.
  
  
  RESULTS
  We selected F2 plants homozygous for the Tv-1 and Tv-2 QTL regions and did three successive backcrosses without phenotypic selection. Twenty-three F2BC3 plants were phenotyped for whitefly resistance and differences were found in oviposition rate of B. tabaci. The F2BC3 plants with the lowest oviposition rate had an introgression on Chromosome 5 in common. Further F2BC4, F2BC4S1 and F2BC4S2 families were developed, genotyped and phenotyped for adult survival, oviposition rate and trichome type and density. It was possible to confirm that an introgression on top of Chr. 5 (OR-5), between the markers rs-2009 and rs-7551, was responsible for reducing whitefly oviposition rate.
  
  
  CONCLUSIONS
  We found a region of 3.06 Mbp at the top of Chr. 5 (OR-5) associated with a reduction in the oviposition rate of B. tabaci. This reduction was independent of the presence of the QTLs Tv-1 and Tv-2 as well as of the presence of trichomes type IV. The OR-5 locus will provide new opportunities for resistance breeding against whiteflies, which is especially relevant in greenhouse cultivation.
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700, AJ, Wageningen, The Netherlands. alejandro.lucatti@bayer.com.|Graduate School Experimental Plant Sciences, Wageningen Campus. Droevendaalsesteeg 1, 6708, PB, Wageningen, The Netherlands. alejandro.lucatti@bayer.com.|Bayer CropScience Vegetable Seeds, Napoleonsweg 152, 6083, AB, Nunhem, The Netherlands. alejandro.lucatti@bayer.com.
authors.forename: Alejandro F
authors.initials: AF
authors.lastname: Lucatti
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700, AJ, Wageningen, The Netherlands. fien.meijer-dekens@wur.nl.
authors.forename: Fien R G
authors.initials: FR
authors.lastname: Meijer-Dekens
authors.affiliation: Plant Research International, Business Unit Bioscience, Wageningen University and Research Centre, P.O. Box 619, 6700, AP, Wageningen, The Netherlands. ronald.mumm@wur.nl.|Netherlands Metabolomics Centre, Einsteinweg 55, 2333, CC, Leiden, The Netherlands. ronald.mumm@wur.nl.
authors.forename: Roland
authors.initials: R
authors.lastname: Mumm
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700, AJ, Wageningen, The Netherlands. richard.visser@wur.nl.
authors.forename: Richard G F
authors.initials: RG
authors.lastname: Visser
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700, AJ, Wageningen, The Netherlands. ben.vosman@wur.nl.
authors.forename: Ben
authors.initials: B
authors.lastname: Vosman
authors.affiliation: Wageningen UR Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700, AJ, Wageningen, The Netherlands. sjaak.vanheusden@wur.nl.
authors.forename: Sjaak
authors.initials: S
authors.lastname: van Heusden
mesh_terms: D000818:Animals; D005260:Female; D017343:Genes, Plant; D056726:Genetic Association Studies; D006430:Hemiptera / Q000502:physiology*; D060434:Herbivory; D018551:Solanum lycopersicum / Q000235:genetics*; D010058:Oviposition; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D032322:Solanum / Q000235:genetics*