title: Amphiphilic PEO-b-PBLG diblock and PBLG-b-PEO-b-PBLG triblock copolymer based nanoparticles: doxorubicin loading and in vitro evaluation.
abstract: Huisgen's 1,3-dipolar cycloaddition ("Click Chemestry") has been used to prepare amphiphilic PEO-b-PBLG diblock and PBLG-b-PEO-b-PBLG triblock copolymers as potential carriers of anticancer drugs. Spherical and flower shaped micelles (D ≈ 100 nm) were obtained from diblock and triblock copolymers respectively. DOX was effectively encapsulated up to 18 wt.% and 50-60% of it was steadily released from the micelles over a period of 7 d. Flow cytometry and fluorescence microscopy confirmed the effective intracellular uptake as well as the sustained release of DOX from micelles. These results suggest that the diblock as well as triblock copolymers are promising carriers for intra-cellular drug delivery.
authors.affiliation: Université de Bordeaux/Bordeaux-INP, ENSCBP CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey Berland, 33607, Pessac Cedex, France; Institute of Nuclear Medicine and Allied Sciences, Brig. S.K. Mazumdar Road, Timarpur, Delhi, 110054, India; Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India; CNRS, Laboratoire de Chimie des Polymeres Organiques (UMR5629), Pessac, France.
authors.forename: Dipti
authors.initials: D
authors.lastname: Kakkar
authors.forename: Silvia
authors.initials: S
authors.lastname: Mazzaferro
authors.forename: Julie
authors.initials: J
authors.lastname: Thevenot
authors.forename: Christophe
authors.initials: C
authors.lastname: Schatz
authors.forename: Anant
authors.initials: A
authors.lastname: Bhatt
authors.forename: Bilikere S
authors.initials: BS
authors.lastname: Dwarakanath
authors.forename: Harpal
authors.initials: H
authors.lastname: Singh
authors.forename: Anil K
authors.initials: AK
authors.lastname: Mishra
authors.forename: Sebastien
authors.initials: S
authors.lastname: Lecommandoux
mesh_terms: D001704:Biopolymers / Q000737:chemistry*; D057930:Click Chemistry; D004317:Doxorubicin; D004337:Drug Carriers / Q000737:chemistry*; D005434:Flow Cytometry; D066298:In Vitro Techniques; D015394:Molecular Structure; D050997:Nanomedicine / Q000379:methods* / Q000639:trends; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D001704:Biopolymers; D004337:Drug Carriers; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011092:Polyethylene Glycols; D004317:Doxorubicin
keywords: PBLG; PEO; block-copolymers; click chemistry; nanoparticles