title: Telencephalin protects PAJU cells from amyloid beta protein-induced apoptosis by activating the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway.
abstract: Telencephalin is a neural glycoprotein that reduces apoptosis induced by amyloid beta protein in the human neural tumor cell line PAJU. In this study, we examined the role of the ezrin/radixin/moesin protein family/phosphatidylinositol-3-kinase/protein kinase B pathway in this process. Western blot analysis demonstrated that telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B were not expressed in PAJU cells transfected with empty plasmid, while they were expressed in PAJU cells transfected with a telencephalin expression plasmid. After treatment with 1.0 nM amyloid beta protein 42, expression of telencephalin and phosphorylated phosphatidylinositol-3-kinase/protein kinase B in the transfected cells gradually diminished, while levels of phosphorylated ezrin/radixin/moesin increased. In addition, the high levels of telencephalin, phosphorylated ezrin/radixin/moesin and phosphatidylinositol-3-kinase/protein kinase B expression in PAJU cells transfected with a telencephalin expression plasmid could be suppressed by the phosphatidylinositol-3-kinase inhibitor LY294002. These findings indicate that telencephalin activates the ezrin/radixin/moesin family/phosphatidylinositol-3-kinase/protein kinase B pathway and protects PAJU cells from amyloid beta protein-induced apoptosis.
authors.affiliation: Department of Neurology, Shangrao No. 5 People's Hospital, the Second Affiliated Hospital of Nanchang University, Shangrao 334000, Jiangxi Province, China ; Department of Neurology, Ruikang Hospital Affiliated to Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China ; Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Heping
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Psychiatry, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Neurology, Xiangya Second Hospital, Central South University, Changsha 410011, Hunan Province, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Hu
keywords: amyloid beta protein; ezrin/radixin/moesin family proteins/phosphatidylinositol-3-kinase/protein kinase B signal transduction; neural regeneration; telencephalin/intercellular adhesion molecule 5