title: Association of polymorphisms in glutathione S-transferase genes (GSTM1, GSTT1, GSTP1) with idiopathic azoospermia or oligospermia in Sichuan, China.
abstract: The reported effects of the glutathione S-transferase (GSTs) genes (GSTM1, GSTT1, and GSTP1) on male factor infertility have been inconsistent and even contradictory. Here, we conducted a case-control study to investigate the association between functionally important polymorphisms in GST genes and idiopathic male infertility. The study group consisted of 361 men with idiopathic azoospermia, 118 men with idiopathic oligospermia, and 234 age-matched healthy fertile male controls. Genomic DNA was extracted from the peripheral blood, and analyzed by polymerase chain reaction and restriction fragment length polymorphism analysis. There was a significant association between the GSTP1 variant genotype (Ile/Val + Val/Val) with idiopathic infertility risk (odds ratio [OR]: 1.53; 95% confidence interval [CI]: 1.11-2.11; P = 0.009). Similarly, a higher risk of infertility was noted in individuals carrying a genotype combination of GSTT1-null and GSTP1 (Ile/Val + Val/Val) (OR: 2.17; 95% CI: 1.43-3.31; P = 0.0002). These results suggest an increased risk of the GSTP1 variant genotype (Ile/Val + Val/Val) for developing male factor infertility. Our findings also underrate the significance of the effect of GSTM1 and/or GSTT1 (especially the former) in modulating the risk of male infertility in males from Sichuan, Southwest China.
authors.forename: Da-Ke
authors.initials: DK
authors.lastname: Xiong
authors.forename: Hong-Han
authors.initials: HH
authors.lastname: Chen
authors.affiliation: Key Laboratory of Bio Resources and Eco-Environment, Ministry of Education, School of Life Science, Institute of Medical Genetics, Sichuan University; Bio-resource Research and Utilization Joint Key Laboratory of Sichuan and Chongqing, Chengdu; Biotechnology Academy of Nanchuan, Chongqing, China.
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Ding
authors.forename: Shao-Hong
authors.initials: SH
authors.lastname: Zhang
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000328:Adult; D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D053713:Azoospermia / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D001483:Base Sequence; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D007248:Infertility, Male / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009845:Oligospermia / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D012307:Risk Factors
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human