title: Protective role of caffeic acid phenethyl ester on serum cholinesterase inhibition by acute exposure to diazinon in rats.
abstract: OBJECTIVE
  To evaluate whether caffeic acid phenethyl ester (CAPE), a flavonoid-like natural compound plentifully found in beeswax, has a protective effect on diazinon-induced serum cholinesterase (ChE) inhibition in rats.
  
  
  METHODS
  Animals were divided into 4 groups. The first animal group was not treated with any substance. The second animal group was orally given a 200 mg/kg body weight (bw) sublethal dose of diazinon. The third animal group was injected intraperitoneally with 2.84 mg (10 micromol)/kg bw of CAPE 1 day prior to administration of 200 mg/kg bw of diazinon orally. The fourth animal group was intraperitoneally injected with 2.84 mg (10 tmol)/kg bw of CAPE 30 min after 200 mg/kg bw of diazinon was orally administered.
  
  
  RESULTS
  Analysis of the animal blood samples obtained 48 h after diazinon administration revealed that diazinon decreased serum ChE activity by 75%, while CAPE administration 24 h prior to and 30 min following diazinon application improved serum ChE activity by 25%-32% as compared to levels with diazinon administration only. In silico studies suggest that CAPE prevents diazinon from binding to butyryl ChE due to a higher binding affinity than that of diazinon.
  
  
  CONCLUSIONS
  Our laboratory findings suggest that CAPE plays a protective role against butyryl ChE inhibition by diazinon.
authors.affiliation: Department of Anesthesiology and Reanimation, Süleyman Demirel University, Isparta, Turkey.
authors.forename: Esin
authors.initials: E
authors.lastname: Oğuzhanoğlu
authors.forename: Ahmet Cenk
authors.initials: AC
authors.lastname: Andaç
authors.forename: Adnan
authors.initials: A
authors.lastname: Tüfek
authors.forename: Lütfi
authors.initials: L
authors.lastname: Yavuz
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Vural
authors.forename: Osman
authors.initials: O
authors.lastname: Gökalp
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002802:Cholinesterases / Q000097:blood* / Q000737:chemistry; D003976:Diazinon / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D008297:Male; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013816:Thermodynamics
chemical_list: D002109:Caffeic Acids; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D002802:Cholinesterases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol; D003976:Diazinon