title: Synthesis, anti-oxidant activity, and biodegradability of a novel recombinant polysaccharide derived from chitosan and lactose.
abstract: A novel recombinant polysaccharide (RP) based on polysaccharide-disaccharide was synthesized from oligo-chitosan (oligo-CS) and reducing lactose using Maillard reaction with the yield of 85.1%. Chemical structure and thermal stability of RP was characterized by Fourier transform infrared spectrum (FT-IR), solid-state nuclear magnetic resonance spectroscopy (CP/MAS (13)C-NMR), and thermo gravimetric analysis (TGA). The anti-oxidant activity of RP was preliminarily investigated by its scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Biodegradability of RP was also examined by the observation of growth status of Aspergillus niger colony. It was demonstrated that RP achieved excellent radical-scavenging efficiency (>80%) at high concentrations of DPPH and its scavenging ability was superior to that of CS, suggesting that anti-oxidant property of CS was remarkably promoted by chemical modification with reducing lactose via Maillard reaction. And biodegradation test revealed that RP had better biodegradability than CS.
authors.affiliation: Department of Chemistry, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China. Electronic address: tdky@hotmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Guo
authors.affiliation: Department of Chemistry, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; School of Engineering, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Chemistry, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China.
authors.forename: Chunge
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Engineering, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; Key Laboratory of Wood Science and Technology, Hangzhou, Zhejiang Province, 311300, China. Electronic address: hzliu@iccas.ac.cn.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Engineering, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Chemistry, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China; National Engineering and Technology Research Center of Wood-based Resources Comprehensive Utilization, Zhejiang Agricultural & Forestry University, Hangzhou, Zhejiang, 311300, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Fei
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis* / Q000737:chemistry*; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry*; D007785:Lactose / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
chemical_list: D000975:Antioxidants; D011134:Polysaccharides; D048271:Chitosan; D007785:Lactose
keywords: Anti-oxidation; Chitosan; Lactose; Recombinant polysaccharide