title: Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy.
abstract: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specifically, VB-ODN for Nuclear factor-κB (NF-κB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-κB itself. The specific binding between VB-ODN produced double-stranded RNA and NF-κB was evidenced by electrophoretic mobility shift assay. Moreover, similar VB-ODNs designed for three other TFs also inhibit their downstream gene expression but not that of themselves. Our study provides a new design of decoy for blocking TF activity.
authors.affiliation: State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China; Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gang
authors.affiliation: No. 518 Hospital of Chinese People's Liberation Army, Xi'an 710043, Shaanxi Province, PR China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Genome Institute, Washington University in St. Louis, St. Louis, MO 63108, USA.
authors.forename: Jiayin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China. Electronic address: lxuhelen@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, PR China. Electronic address: liuzhiguo@fmmu.edu.cn.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D002470:Cell Survival; D015870:Gene Expression; D005822:Genetic Vectors / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D008954:Models, Biological; D016328:NF-kappa B / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000378:metabolism*; D015203:Reproducibility of Results; D047490:Retinoid X Receptor alpha / Q000378:metabolism; D050656:Transcription Factor AP-2 / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D016760:Proto-Oncogene Proteins c-fos; D012330:RNA, Double-Stranded; D047490:Retinoid X Receptor alpha; D050656:Transcription Factor AP-2; D014157:Transcription Factors
keywords: NF-κB; Transcription factor decoy; VB-ODN; shRNA