title: Breaking the host range: mandarin fish is susceptible to a vesiculovirus derived from snakehead fish.
abstract: Members of the genus Vesiculovirus, which belongs to the family Rhabdoviridae, can cause great economic loss in fish culture. In the present report, a vesiculovirus [named snakehead fish vesiculovirus (SHVV)] was isolated from diseased hybrid snakehead fish. SHVV shared 94 % nucleotide sequence identity at the genomic level with Siniperca chuatsi rhabdovirus (SCRV), which infects mandarin fish (S. chuatsi). We showed that SHVV was able to replicate and proliferate well in SSN-1 cells, which originate from striped snakehead fish (Channa striatus). Furthermore, mandarin fish was susceptible to SHVV by bath exposure, as well as by intraperitoneal injection. The infected fish showed typical clinical signs of rhabdovirus infection, including haemorrhage and oedema. Histopathological analysis revealed that extensive inflammation and necrosis were observed in the spleen, kidney, liver, heart and brain of the moribund mandarin fish. These results will shed new light on the epidemic of vesiculovirus infections among fish.
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.|Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Division of Science and Technology, Beijing Normal University - Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.|Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Xianqin
authors.initials: X
authors.lastname: Hu
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.|Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.
authors.forename: Xufang
authors.initials: X
authors.lastname: Liang
authors.affiliation: School of Biological Sciences, Lake Superior State University, Sault Ste. Marie, MI 49783, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Marine and Environmental Technology, University of Maryland, Baltimore, MD 21202, USA.
authors.forename: Vikram
authors.initials: V
authors.lastname: Vakharia
authors.affiliation: Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, PR China.|Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, PR China.|Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation / Q000235:genetics; D005393:Fish Diseases / Q000821:virology*; D058507:Host Specificity; D008969:Molecular Sequence Data; D010473:Perciformes; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D018116:Vesiculovirus / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication / Q000235:genetics