title: The relative importance of the adsorption and partitioning mechanisms in hydrophilic interaction liquid chromatography.
abstract: We propose an original model of effective diffusion along packed beds of mesoporous particles for HILIC developed by combining Torquatos model for heterogeneous beds (external eluent+particles), Landauers model for porous particles (solid skeleton+internal eluent), and the time-averaged model for the internal eluent (bulk phase+diffuse water (W) layer+rigid W layer). The new model allows to determine the analyte concentration in rigid and diffuse W layer from the experimentally determined retention factor and intra-particle diffusivity and thus to distinguish the retentive contributions from adsorption and partitioning. We apply the model to investigate the separation of toluene (TO, as a non-retained compound), nortriptyline (NT), cytosine (CYT), and niacin (NA) on an organic ethyl/inorganic silica hybrid adsorbent. Elution conditions are varied through the choice of a third solvent (W, ethanol, tetrahydrofuran (THF), acetonitrile (ACN), or n-hexane) in a mobile phase (MP) of ACN/aqueous acetate buffer (pH 5)/third solvent (90/5/5, v/v/v). Whereas NA and CYT retention factors increase monotonously from W to n-hexane as third solvent, NT retention reaches its maximum with polar aprotic third solvents. The involved equilibrium constants for adsorption and partitioning, however, do not follow the same trends as the overall retention factors. NT retention is dominated by partitioning and NA retention by adsorption, while CYT retention is controlled by adsorption rather than partitioning. Our results reveal that the relative importance of adsorption and partitioning mechanisms depends in a complex way from analyte properties and experimental parameters and cannot be predicted generally.
authors.affiliation: Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA. Electronic address: gritti@ion.chem.utk.edu.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Gritti
authors.affiliation: Department of Chemistry, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Höltzel
authors.affiliation: Department of Chemistry, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Tallarek
authors.affiliation: Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
authors.forename: Georges
authors.initials: G
authors.lastname: Guiochon
mesh_terms: D000327:Adsorption; D002021:Buffers; D002853:Chromatography, Liquid / Q000379:methods*; D003596:Cytosine / Q000302:isolation & purification; D004058:Diffusion; D057927:Hydrophobic and Hydrophilic Interactions; D009525:Niacin / Q000302:isolation & purification; D009661:Nortriptyline / Q000302:isolation & purification; D016062:Porosity; D012822:Silicon Dioxide; D012997:Solvents; D014050:Toluene / Q000302:isolation & purification
chemical_list: D002021:Buffers; D012997:Solvents; D009525:Niacin; D014050:Toluene; D012822:Silicon Dioxide; D003596:Cytosine; D009661:Nortriptyline
keywords: Adsorption–partitioning mechanism; HILIC; Intra-particle diffusivity; Landauer's model; Retention mechanism; Torquato's model