title: Microfluidic perfusion culture system for multilayer artery tissue models.
abstract: We described an assembly technique and perfusion culture system for constructing artery tissue models. This technique differed from previous studies in that it does not require a solid biodegradable scaffold; therefore, using sheet-like tissues, this technique allowed the facile fabrication of tubular tissues can be used as model. The fabricated artery tissue models had a multilayer structure. The assembly technique and perfusion culture system were applicable to many different sizes of fabricated arteries. The shape of the fabricated artery tissue models was maintained by the perfusion culture system; furthermore, the system reproduced the in vivo environment and allowed mechanical stimulation of the arteries. The multilayer structure of the artery tissue model was observed using fluorescent dyes. The equivalent Young's modulus was measured by applying internal pressure to the multilayer tubular tissues. The aim of this study was to determine whether fabricated artery tissue models maintained their mechanical properties with developing. We demonstrated both the rapid fabrication of multilayer tubular tissues that can be used as model arteries and the measurement of their equivalent Young's modulus in a suitable perfusion culture environment.
authors.affiliation: Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University , 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yamagishi
authors.affiliation: Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University , 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Masuda
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University , 2-1 Yamadaoka, Suita, Osaka 562-0871, Japan.
authors.forename: Michiya
authors.initials: M
authors.lastname: Matsusaki
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Osaka University , 2-1 Yamadaoka, Suita, Osaka 562-0871, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Akashi
authors.affiliation: Department of Cardiovascular Research Institute, Graduate School of Medicine, Yokohama City University , 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
authors.forename: Utako
authors.initials: U
authors.lastname: Yokoyama
authors.affiliation: Department of Micro-Nano Systems Engineering, Graduate School of Engineering, Nagoya University , 1 Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
authors.forename: Fumihito
authors.initials: F
authors.lastname: Arai