title: OsGL1-3 is involved in cuticular wax biosynthesis and tolerance to water deficit in rice.
abstract: Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was ubiquitously expressed at different level in rice plants except root and its expression was up-regulated under ABA and PEG treatments. The transient expression of OsGL1-3-GFP fusion protein indicated that OsGL1-3 is mainly localized in the plasma membrane. Compared to the wild type, overexpression rice plants exhibited stunted growth, more wax crystallization on leaf surface, and significantly increased total cuticular wax load due to the prominent changes of C30-C32 aldehydes and C30 primary alcohols. While the RNAi knockdown mutant of OsGL1-3 exhibited no significant difference in plant height, but less wax crystallization and decreased total cuticular wax accumulation on leaf surface. All these evidences, together with the effects of OsGL1-3 on the expression of some wax synthesis related genes, suggest that OsGL1-3 is involved in cuticular wax biosynthesis. Overexpression of OsGL1-3 decreased chlorophyll leaching and water loss rate whereas increased tolerance to water deficit at both seedling and late-tillering stages, suggesting an important role of OsGL1-3 in drought tolerance.
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Linzhi
authors.initials: L
authors.lastname: Li
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; School of Life Science, Hunan University of Science and Technology, Xiangtan, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; Research Institute of Science and Technology Information, Hunan Academy of Agricultural Sciences, Changsha, China.
authors.forename: Guofu
authors.initials: G
authors.lastname: Gao
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Faxi
authors.initials: F
authors.lastname: Xu
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Ailing
authors.initials: A
authors.lastname: Liu
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, China.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Crop Germplasm Innovation and Utilization of Hunan Province, Hunan Agricultural University, Changsha, China; State Key laboratory of Main Crop Germplasm Innovation, Beijing, China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Wan
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000222:Adaptation, Physiological; D002463:Cell Membrane Permeability; D002734:Chlorophyll / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D019441:Plant Epidermis / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D014867:Water / Q000378:metabolism*; D014885:Waxes / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014885:Waxes; D014867:Water; D002734:Chlorophyll; D000040:Abscisic Acid