title: Effects of granular activated carbon on methane removal performance and methanotrophic community of a lab-scale bioreactor.
abstract: Two identical lab-scale bioreactor systems were operated to examine the effects of granular activated carbon (GAC) on methane removal performance and methanotrophic community. Both bioreactor systems removed methane completely at a CH4 loading rate of 71.2 g-CH4·d(-1) for 17 days. However, the methane removal efficiency declined to 88% in the bioreactor without GAC, while the bioreactor amended with GAC showed greater methane removal efficiency of 97% at a CH4 loading rate of 107.5 g-CH4·d(-1). Although quantitative real-time PCR showed that methanotrophic populations were similar levels of 5-10 × 10(8) pmoA gene copy number·VSS(-1) in both systems, GAC addition changed the methanotrophic community composition of the bioreactor systems. Microarray assay revealed that GAC enhanced the type I methanotrophic genera including Methylobacter, Methylomicrobium, and Methylomonas of the system, which suggests that GAC probably provided a favorable environment for type I methanotrophs. These results indicated that GAC is a promising support material in bioreactor systems for CH4 mitigation.
authors.affiliation: a Department of Environmental Science and Engineering , Ewha Womans University , Seoul , Republic of Korea.
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Lee
authors.forename: Sun-Ah
authors.initials: SA
authors.lastname: Choi
authors.forename: Taewoo
authors.initials: T
authors.lastname: Yi
authors.forename: Tae Gwan
authors.initials: TG
authors.lastname: Kim
authors.forename: Sang-Don
authors.initials: SD
authors.lastname: Lee
authors.forename: Kyung-Suk
authors.initials: KS
authors.lastname: Cho
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D002606:Charcoal; D008697:Methane / Q000378:metabolism*; D042082:Methylobacteriaceae / Q000378:metabolism*; D008772:Methylococcaceae / Q000378:metabolism*; D056910:Republic of Korea; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D002606:Charcoal; D008697:Methane
keywords: Bioreactor; granular activated carbon; methane; methanotrophs