title: Analysis of psilocin, bufotenine and LSD in hair.
abstract: A method for the simultaneous extraction of the hallucinogens psilocin, bufotenine, lysergic acid diethylamide (LSD) as well as iso-LSD, nor-LSD and O-H-LSD from hair with hydrochloride acid and methanol is presented. Clean-up of the hair extracts is performed with solid phase extraction using a mixed-mode cation exchanger. Extracts are measured with liquid chromatography coupled with electrospray tandem mass spectrometry. The method was successfully validated according to the guidelines of the 'Society of Toxicological and Forensic Chemistry' (GTFCh). To obtain reference material hair was soaked in a solution of the analytes in dimethyl sulfoxide/methanol to allow incorporation into the hair. These fortified hair samples were used for method development and can be employed as quality controls.
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, D-48149 Münster, Germany rafaela.martin@uni-muenster.de.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Martin
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, D-48149 Münster, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Schürenkamp
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, D-48149 Münster, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Gasse
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, D-48149 Münster, Germany.
authors.forename: Heidi
authors.initials: H
authors.lastname: Pfeiffer
authors.affiliation: Institute of Legal Medicine, University Hospital Münster, Röntgenstr. 23, D-48149 Münster, Germany.
authors.forename: Helga
authors.initials: H
authors.lastname: Köhler
mesh_terms: D002027:Bufotenin / Q000032:analysis*; D002853:Chromatography, Liquid; D006197:Hair / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008238:Lysergic Acid Diethylamide / Q000032:analysis* / Q000378:metabolism; D011562:Psilocybin / Q000031:analogs & derivatives* / Q000032:analysis; D052616:Solid Phase Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D002027:Bufotenin; D011562:Psilocybin; D008238:Lysergic Acid Diethylamide; C009105:psilocin