title: Comparison of the effects of aerobic conditioning before and after pulmonary allergic inflammation.
abstract: The aim of this study is to compare the effects of aerobic conditioning (AC) before (ACBS) and after (ACAS) allergic sensitization. BALB/c mice were divided into two main groups: ACBS and ACAS. Each groups was divided into subgroups: control (nonsensitized/nontrained), AC (nonsensitized/trained), ovalbumin (OVA) (sensitized/nontrained), AC+OVA (trained/sensitized), and OVA+AC (sensitized/trained). Sensitization was induced using OVA and AC performed in treadmill (moderate intensity). We examined IgE and IgG1 levels, eosinophil counting, expression of Th1 (interleukin (IL)-2, IFN-α) and Th2 cytokines (IL-4, IL-5, IL-13), IL-10, vascular endothelial growth factor (VEGF), and airway remodeling. IgE and IgG1 were decreased only when exercise was performed before sensitization (ACBS); however, there was a decrease of eosinophils, Th2 cytokines, VEGF, and airway remodeling and increase in IL-10 in either ACBS or ACAS groups. Our results demonstrate that aerobic conditioning reduces Th2 response before and after sensitization by increasing IL-10 while the production of anaphylactic antibodies is reduced only when exercise is performed before sensitization.
authors.affiliation: Department of Physical Therapy, University of Sao Paulo, São Paulo, Brazil.
authors.forename: Ronaldo Aparecido
authors.initials: RA
authors.lastname: da Silva
authors.forename: Francine Maria
authors.initials: FM
authors.lastname: Almeida
authors.forename: Clarice Rosa
authors.initials: CR
authors.lastname: Olivo
authors.forename: Beatriz Mangueira
authors.initials: BM
authors.lastname: Saraiva-Romanholo
authors.forename: Adenir
authors.initials: A
authors.lastname: Perini
authors.forename: Milton Arruda
authors.initials: MA
authors.lastname: Martins
authors.forename: Celso Ricardo Fernandes
authors.initials: CR
authors.lastname: Carvalho
mesh_terms: D056151:Airway Remodeling / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism*; D018793:Interleukin-13 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008168:Lung / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin; D010805:Physical Conditioning, Animal; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis
chemical_list: C556062:IL10 protein, mouse; D007074:Immunoglobulin G; D018793:Interleukin-13; D007376:Interleukin-2; D042461:Vascular Endothelial Growth Factor A; C467480:vascular endothelial growth factor A, mouse; D016753:Interleukin-10; D015847:Interleukin-4; D007073:Immunoglobulin E; D010047:Ovalbumin