title: Molecular characterization of E2 glycoprotein of classical swine fever virus: adaptation and propagation in porcine kidney cells.
abstract: Classical swine fever virus (CSFV) is the causative agent of a highly contagious disease, hog cholera in pigs. The disease is endemic in many parts of the world, and vaccination is the only way to protect the animals from CSFV infection. The lapinized vaccine strains are occasionally not protective because of animal to animal passage, inadequate vaccination strategy, suboptimal vaccine dose, and emergence of new variants. The surface glycoprotein E2 of CSFV is a major antigenic determinant and can modulate the disease outcome in pigs. In the present study, we characterized the CSFV in porcine kidney cells. The CSFV vaccine strains showed enhanced replication following 15 passages in porcine kidney cells. Nucleotide sequence analysis of the E2 protein gene of the cell culture-adapted vaccine strain of CSFV showed a mutation in putative amino acid sequences that are identical to its virulent counterpart. The study suggests the possibility of exaltation in vaccine strains following its adaptation in host cells and paves the way for a further exploration of the biology of its outbreak.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India, k.rakesh279@gmail.com.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Nagendra N
authors.initials: NN
authors.lastname: Barman
authors.forename: Elina
authors.initials: E
authors.lastname: Khatoon
authors.forename: Gitika
authors.initials: G
authors.lastname: Rajbongshi
authors.forename: Nipu
authors.initials: N
authors.lastname: Deka
authors.forename: Sudhir
authors.initials: S
authors.lastname: Morla
authors.forename: Sachin
authors.initials: S
authors.lastname: Kumar
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D006691:Classical Swine Fever / Q000235:genetics*; D006692:Classical Swine Fever Virus / Q000235:genetics* / Q000472:pathogenicity; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000235:genetics / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*; D014774:Virulence
chemical_list: D014759:Viral Envelope Proteins; D014765:Viral Vaccines