title: A panel of five serum miRNAs as a potential diagnostic tool for early-stage renal cell carcinoma.
abstract: Circulating microRNAs (miRNAs) are emerging as clinically useful tools for cancer detection; however, little is known about their early diagnostic impact on RCC. The levels of 754 serum miRNAs were initially determined using a TaqMan Low Density Array in two pooled samples from 25 RCC and 25 noncancer controls. Markedly dysregulated miRNAs in RCC cases were subsequently validated individually by qRT-PCR in another 107 patients and 107 controls arranged in two sets. The serum levels of miR-193a-3p, miR-362 and miR-572 were significantly increased whereas the levels of miR-28-5p and miR-378 were markedly decreased in patients with RCC, even in those with stage I disease, compared with the noncancer controls (P < 0.01). The areas under the ROC curve (AUCs) for the 5 combined miRNAs were 0.807 (95% CI, 0.687-0.928) and 0.796 (95% CI, 0.724-0.867) for the training set and the validation set, respectively. Furthermore, the panel enabled the differentiation of stage I RCC from controls with AUC of 0.807 (95% CI, 0.731-0.871), a sensitivity of 80% and a specificity of 71%. This panel of 5 serum miRNA may have the potential to be used clinically as an auxiliary diagnostic tool for the early detection of RCC.
authors.affiliation: 1] Department of Clinical Laboratory, Jinling Hospital, School of Life Sciences, Nanjing University, Nanjing 210002, China [2] Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Urology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Jinling Hospital, School of Life Sciences, Nanjing University, Nanjing 210002, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Lu
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gu
authors.affiliation: Department of Pathology, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zen
authors.affiliation: Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Urology, The Fifth Affiliated Hospital of Harbin Medical University, Daqing, China.
authors.forename: Tiehui
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Urology, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Clinical Laboratory, Jinling Hospital, School of Life Sciences, Nanjing University, Nanjing 210002, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1] Department of Clinical Laboratory, Jinling Hospital, School of Life Sciences, Nanjing University, Nanjing 210002, China [2] Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.
authors.forename: Chunni
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis*; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs