title: Innovative approach for urease inhibition by Ficus carica extract-fabricated silver nanoparticles: An in vitro study.
abstract: In the present study, a rapid, low-cost, and ecofriendly method of stable silver nanoparticles (AgNPs) synthesis using leaves extract of Ficus carica (F. carica), a plant with diverse metabolic consortium, is reported for the first time. An absorption peak at 422 nm in UV-Vis spectroscopy, a spherical shape with an average size of 21 nm in transmission electron microscopy, and crystalline nature in X-ray powder diffraction studies were observed for the synthesized AgNPs. Fourier transform infrared analysis indicated that proteins of F. carica might have a vital role in AgNP synthesis and stabilization. AgNPs were found to inhibit urease, a key enzyme responsible for the survival and pathogenesis of the bacterium, Helicobacter pylori. Inhibition of urease by AgNPs was monitored spectrophotometrically by the evaluation of ammonia release. The urease inhibition potential of AgNPs can be explored in the treatment of H. pylori by preparing novel combinations of standard drugs with AgNPs- or AgNPs-encapsulated drug molecules.
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Hemant P
authors.initials: HP
authors.lastname: Borase
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Rahul B
authors.initials: RB
authors.lastname: Salunkhe
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Chandrashekhar D
authors.initials: CD
authors.lastname: Patil
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Rahul K
authors.initials: RK
authors.lastname: Suryawanshi
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Bipinchandra K
authors.initials: BK
authors.lastname: Salunke
authors.affiliation: School of Environment Science, North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Nilesh D
authors.initials: ND
authors.lastname: Wagh
authors.affiliation: School of Life Sciences, North Maharashtra University, Jalgaon, Maharashtra, India.|North Maharashtra Microbial Culture Collection Centre (NMCC), North Maharashtra University, Jalgaon, Maharashtra, India.
authors.forename: Satish V
authors.initials: SV
authors.lastname: Patil
mesh_terms: D000641:Ammonia / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D030681:Ficus / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D010936:Plant Extracts; D012834:Silver; D000641:Ammonia; D014510:Urease
keywords: FT-IR; Helicobacter pylori; biomaterials; silver nanoparticles; urease inhibitors