title: [Frequency of diseases caused by group A streptococci among invasive infections of soft tissues and characteristics of the causative agent].
abstract: OBJECTIVE
  Determine frequency of diseases caused by group A streptococci (GAS) among invasive infections of soft tissues; identify emm-types of the isolated streptococci, determine the presence of bacteriophage integrases and toxin genes in their genomes.
  
  
  METHODS
  4750 case histories of patients with soft tissue infections of the purulent-surgical department of the 23rd City Clinical Hospital.of Moscow "Medsantrud" in 2008 - 2011 were analyzed. 46 strains of GAS isolated from patients with invasive streptococcus infection (ISI) were studied. GAS identification was carried out by latex-agglutination method. GAS emm-type was determined by molecular-genetic methods, as well as the presence of bacteriophage integrases int2, int3, int4, int5, int6, int7, int49, bacteriophage toxins speA, speI, sla, speC/J, speL, speH, speC, ssa and speB gene present on the chromosomal DNA.
  
  
  RESULTS
  132 cases (2.8%) were attributed to invasive infections. In 46 cases of invasive infections (35%) GAS were isolated. 22 different emm-types of invasive GAS strains were detected. Only speB gene among all the toxin genes (as well as the expression of the gene--SpeB toxin) was detected in all the strains, whereas sla and speI genes were not detected in any of the strains. Genes of the other toxins (ssa, speL, speC, speA, speH, speC/J) occurred in a number of strains. Genes of phage integrases were detected among all the strains however in varying combinations (from 1 to 4 genes).
  
  
  CONCLUSIONS
  Invasive infections caused by GAS are more frequently spread than had been previously assumed and a high degree of genetic heterogeneity of invasive GAS strains was detected.
authors.forename: N I
authors.initials: NI
authors.lastname: Briko
authors.forename: E V
authors.initials: EV
authors.lastname: Glushkova
authors.forename: A G
authors.initials: AG
authors.lastname: Nosik
authors.forename: A V
authors.initials: AV
authors.lastname: Dmitriev
authors.forename: N F
authors.initials: NF
authors.lastname: Dmitrieva
authors.forename: D A
authors.initials: DA
authors.lastname: Kleĭmenov
authors.forename: K V
authors.initials: KV
authors.lastname: Lipatov
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D005098:Exotoxins / Q000096:biosynthesis / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D009031:Moscow; D018461:Soft Tissue Infections / Q000235:genetics / Q000382:microbiology* / Q000473:pathology; D013290:Streptococcal Infections / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D013297:Streptococcus pyogenes / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; C117980:SpeA protein, Streptococcus pyogenes; C027951:erythrogenic toxin