title: Ultrasound enhanced ethanol production from Parthenium hysterophorus: A mechanistic investigation.
abstract: This study presents mechanistic investigations in ultrasound-assisted bioethanol fermentation using Parthenium hysterophorus biomass. Ultrasound (35 kHz, 10% duty cycle) has been used for sonication. Experimental results were fitted to mathematical model; the kinetic and physiological parameters in the model were obtained using Genetic Algorithm (GA) based optimization. In control experiments (mechanical shaking), maximum ethanol titer of 10.93 g/L and cell mass concentration of 5.26 g/L was obtained after 18 h. In test experiments (mechanical shaking and intermittent sonication), ethanol titer of 12.14 g/L and cell mass concentration of 5.7 g/L was obtained in 10h. This indicated ∼ 2 × enhanced productivity of ethanol and cell mass with sonication. Trends in model parameters obtained after fitting of model to experimental data essentially revealed that beneficial influence of ultrasound on fermentation is a manifestation of enhanced trans-membrane transportation and dilution of toxic substances due to strong micro-convection induced by ultrasound.
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Shuchi
authors.initials: S
authors.lastname: Singh
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Shyamali
authors.initials: S
authors.lastname: Sarma
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Mayank
authors.initials: M
authors.lastname: Agarwal
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India; Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
authors.affiliation: Center for Energy, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India; Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India. Electronic address: vmoholkar@iitg.ernet.in.
authors.forename: Vijayanand S
authors.initials: VS
authors.lastname: Moholkar
mesh_terms: D000465:Algorithms; D001234:Aspergillus niger / Q000201:enzymology; D019659:Asteraceae / Q000737:chemistry*; D001407:Bacillus / Q000201:enzymology; D056804:Biofuels; D018533:Biomass; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry*; D005285:Fermentation; D007218:Industrial Microbiology; D007700:Kinetics; D008962:Models, Theoretical; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013010:Sonication; D014465:Ultrasonics; D001617:beta-Glucosidase / Q000737:chemistry
chemical_list: D056804:Biofuels; D000431:Ethanol; D001617:beta-Glucosidase; D002266:Carboxymethylcellulose Sodium
keywords: Bioethanol; Fermentation; Mathematical modeling; Parthenium hysterophorus; Sonication