title: Transposon mutagenesis identifies genes and evolutionary forces driving gastrointestinal tract tumor progression.
abstract: To provide a more comprehensive understanding of the genes and evolutionary forces driving colorectal cancer (CRC) progression, we performed Sleeping Beauty (SB) transposon mutagenesis screens in mice carrying sensitizing mutations in genes that act at different stages of tumor progression. This approach allowed us to identify a set of genes that appear to be highly relevant for CRC and to provide a better understanding of the evolutionary forces and systems properties of CRC. We also identified six genes driving malignant tumor progression and a new human CRC tumor-suppressor gene, ZNF292, that might also function in other types of cancer. Our comprehensive CRC data set provides a resource with which to develop new therapies for treating CRC.
authors.affiliation: 1] Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore. [2] Department of Oncologic Pathology, School of Medicine, Kanazawa Medical University, Ishikawa, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Takeda
authors.affiliation: Cancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
authors.forename: Zhubo
authors.initials: Z
authors.lastname: Wei
authors.affiliation: 1] Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore. [2] Division of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Hideto
authors.initials: H
authors.lastname: Koso
authors.affiliation: Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Hinxton, UK.
authors.forename: Alistair G
authors.initials: AG
authors.lastname: Rust
authors.affiliation: Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.
authors.forename: Christopher Chin Kuan
authors.initials: CC
authors.lastname: Yew
authors.affiliation: 1] Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore. [2] Cancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
authors.forename: Michael B
authors.identifier: 0000000275156515
authors.initials: MB
authors.lastname: Mann
authors.affiliation: Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.
authors.forename: Jerrold M
authors.initials: JM
authors.lastname: Ward
authors.affiliation: Experimental Cancer Genetics, Wellcome Trust Sanger Institute, Hinxton, UK.
authors.forename: David J
authors.initials: DJ
authors.lastname: Adams
authors.affiliation: 1] Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore. [2] Cancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
authors.forename: Neal G
authors.initials: NG
authors.lastname: Copeland
authors.affiliation: 1] Division of Genomics and Genetics, Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore. [2] Cancer Research Program, Houston Methodist Research Institute, Houston, Texas, USA.
authors.forename: Nancy A
authors.initials: NA
authors.lastname: Jenkins
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D000236:Adenoma / Q000235:genetics* / Q000473:pathology; D000818:Animals; D005075:Biological Evolution; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016254:Mutagenesis, Insertional / Q000379:methods*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D013047:Specific Pathogen-Free Organisms; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D002352:Carrier Proteins; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors; C570380:ZNF292 protein, human; C406423:Zfp292 protein, mouse