title: Hypoglycaemic effects of functional tri-peptides from silk in differentiated adipocytes and streptozotocin-induced diabetic mice.
abstract: BACKGROUND
  In this study, the tri-peptides Gly-Glu-Tyr (GEY) and Gly-Tyr-Gly (GYG), identified previously as active compounds from the silk peptide E5K6, significantly stimulated basal and insulin-mediated glucose uptake by 3T3-L1 fibroblasts in a dose-dependent manner.
  
  
  RESULTS
  Synthetic GEY and GYG peptides at a concentration of 500 µmol L(-1) significantly increased glucose transporter type 4 expression by 157% and 239%, respectively. Differentiation of 3T3-L1 cells into adipocytes leads to accumulation of intracellular fat droplets, and GEY and GYG at a concentration of 250 µmol L(-1) suppressed this effect by 72% and 75%, respectively. GYG improved glucose tolerance in steptozotocin (STZ)-induced diabetic mice in a dose-dependent manner.
  
  
  CONCLUSIONS
  These results suggest that GYG isolated from E5K6 has anti-diabetic potential and silk waste products containing bioactive peptides could be used to the developments of treatments to lower blood glucose.
authors.affiliation: BK Bio Co. Ltd, Sungnam, 462-819, Republic of Korea.
authors.forename: Bok Kyung
authors.initials: BK
authors.lastname: Han
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Food Quality & Safety Department, Agency for Korea National Food Cluster, Gwacheon, 427-806, Republic of Korea.
authors.forename: Hyun-Sun
authors.initials: HS
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D018149:Glucose Intolerance / Q000188:drug therapy* / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009842:Oligopeptides / Q000494:pharmacology / Q000627:therapeutic use*; D047011:Silk / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D009842:Oligopeptides; D047011:Silk; C495693:Slc2a4 protein, mouse; C078834:glycyl-tyrosyl-glycine
keywords: 3T3-L1 adipocyte; STZ-induced diabetic mice; alcalase; glucose uptake; silk peptide E5K6