title: Inhibition of type 1 fimbriae-mediated Escherichia coli adhesion and biofilm formation by trimeric cluster thiomannosides conjugated to diamond nanoparticles.
abstract: Recent advances in nanotechnology have seen the development of a number of microbiocidal and/or anti-adhesive nanoparticles displaying activity against biofilms. In this work, trimeric thiomannoside clusters conjugated to nanodiamond particles (ND) were targeted for investigation. NDs have attracted attention as a biocompatible nanomaterial and we were curious to see whether the high mannose glycotope density obtained upon grouping monosaccharide units in triads might lead to the corresponding ND-conjugates behaving as effective inhibitors of E. coli type 1 fimbriae-mediated adhesion as well as of biofilm formation. The required trimeric thiosugar clusters were obtained through a convenient thiol-ene "click" strategy and were subsequently conjugated to alkynyl-functionalized NDs using a Cu(I)-catalysed "click" reaction. We demonstrated that the tri-thiomannoside cluster-conjugated NDs (ND-Man3) show potent inhibition of type 1 fimbriae-mediated E. coli adhesion to yeast and T24 bladder cells as well as of biofilm formation. The biofilm disrupting effects demonstrated here have only rarely been reported in the past for analogues featuring such simple glycosidic motifs. Moreover, the finding that the tri-thiomannoside cluster (Man3N3) is itself a relatively efficient inhibitor, even when not conjugated to any ND edifice, suggests that alternative mono- or multivalent sugar-derived analogues might also be usefully explored for E. coli-mediated biofilm disrupting properties.
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI, USR CNRS 3078), Université Lille 1, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France. Sabine.Szunerits@iri.univ-lille1.fr.
authors.forename: Manakamana
authors.initials: M
authors.lastname: Khanal
authors.forename: Fanny
authors.initials: F
authors.lastname: Larsonneur
authors.forename: Victoriia
authors.initials: V
authors.lastname: Raks
authors.forename: Alexandre
authors.initials: A
authors.lastname: Barras
authors.forename: Jean-Sébastien
authors.initials: JS
authors.lastname: Baumann
authors.forename: Fernando Ariel
authors.initials: FA
authors.lastname: Martin
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Ghigo
authors.forename: Carmen
authors.initials: C
authors.lastname: Ortiz Mellet
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zaitsev
authors.forename: Jose M
authors.initials: JM
authors.lastname: Garcia Fernandez
authors.forename: Christophe
authors.initials: C
authors.lastname: Beloin
authors.forename: Aloysius
authors.initials: A
authors.lastname: Siriwardena
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry; D018441:Biofilms; D002241:Carbohydrates / Q000737:chemistry; D045744:Cell Line, Tumor; D002855:Chromatography, Thin Layer; D018130:Diamond / Q000737:chemistry*; D019281:Dimerization; D004926:Escherichia coli / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008362:Mannosides / Q000737:chemistry*; D058726:Nanoconjugates; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D056951:Photoelectron Spectroscopy; D012997:Solvents / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D002241:Carbohydrates; D006027:Glycosides; D008362:Mannosides; D058726:Nanoconjugates; D012997:Solvents; C008245:lactosides; D049452:Green Fluorescent Proteins; D018130:Diamond