title: Proteomics analysis for enhanced lipid accumulation in oleaginous Chlorella vulgaris under a heterotrophic-Na⁺ induction two-step regime.
abstract: A heterotrophic-Na(+) induction (HNI) two-step regime was developed to enhance lipid accumulation in oleaginous Chlorella vulgaris. C. vulgaris was cultivated heterotrophically to a biomass of 7.8 g l(-1) in 120 h. The cells were re-suspended in fresh media supplemented with 0.5 M NaCl followed by 12 h growth to accumulate lipid to 53.4 % (w/w). The lipid productivity (625 mg l(-1) day(-1)) achieved with HNI was better than that using heterotrophy alone (405 mg l(-1) day(-1)). To promote possible applications of HNI strategy in other microalgal species, the lipid triggers and potential molecular pathways associated with lipid biosynthesis were investigated. Malic enzyme and acyl-CoA-binding protein were key metabolic checkpoints found to modulate lipid biosynthesis in cells. These results provide the foundation to develop high-lipid engineering miroalgae for industrialization of biodiesel.
authors.affiliation: School of Chemical Engineering, Xiangtan University, Xiangtan, 411105, People's Republic of China, yuqinli2004@126.com.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Li
authors.forename: Jinxiu
authors.initials: J
authors.lastname: Mu
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Xu
authors.forename: Fangxin
authors.initials: F
authors.lastname: Han
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D020418:Algal Proteins / Q000032:analysis*; D048408:Chlorella vulgaris / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry; D050356:Lipid Metabolism / Q000187:drug effects*; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D020543:Proteome / Q000032:analysis*; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D020418:Algal Proteins; D003470:Culture Media; D020543:Proteome; D012965:Sodium Chloride