title: Physicochemical standardization, HPTLC profiling, and biological evaluation of Aśvagandhādyariṣṭa: A comparative study of three famous commercial brands.
abstract: BACKGROUND
  Aśvagandhādyariṣṭa is a polyherbal formulation that is available commercially as an over the counter drug. There are three famous brands that are available in the market. However, there are no comparative reports on the physicochemical, chromatographic, and biological profiles of Aśvagandhādyariṣṭa manufactured by these famous companies.
  
  
  OBJECTIVE
  The present study deals with the physicochemical standardization, high performance thin layer chromatography (HPTLC) profiling, and biological evaluation of Aśvagandhādyariṣṭa.
  
  
  METHODS
  Aśvagandhādyariṣṭa manufactured by three leading companies were purchased from Jalandhar, Punjab. The physicochemical standardization of the samples was carried out in accordance with the Ayurvedic Pharmacopoeia of India (API). Authentified Eisenia foetida were procured from Ujjwal Ujala Vermiculture Group, Amritsar. The anthelmintic activity, 1,1-diphenyl-2-picrylhydrazyl scavenging, and hydrogen peroxide scavenging ability of Aśvagandhādyariṣṭa was determined.
  
  
  METHODS
  The data of anthelmintic activity were expressed as mean ± standard error of the mean of six earthworms in each group. The statistical analysis was carried out using one-way analysis of variance, followed by Dunnet t-test. The difference in values at P < 0.05 was considered to be statistically significant.
  
  
  RESULTS
  Most of the physicochemical standardization parameters mentioned as per the API were found to be within limit. HPTLC profiling showed the presence of withanolide D in commercial samples. Out of three commercial brands, ASA-DAB was the most active as compared to the ASA-BDN and ASA-AVP at the concentration of 200 mg/ml for anthelmintic activity against E. foetida. ASA-DAB showed the best antioxidant activity in both the in vitro assay at the concentration of 100 μg/ml.
  
  
  CONCLUSIONS
  The ability of this formulation to scavenge free radicals supports its medical claim of antistress formulation. The anthelmintic potential of this formulation helps us conclude that it can also be considered as a general tonic because it provides relief from helminths.
authors.affiliation: Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Singh
authors.affiliation: Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India.
authors.forename: Navdeep
authors.initials: N
authors.lastname: Kaur
authors.affiliation: Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab, India ; Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Rajasthan, India.
authors.forename: Atish Tulsiram
authors.initials: AT
authors.lastname: Paul
keywords: 1,1-diphenyl-2- picrylhydrazyl; Anthelmintic; Aśvagandhādyariṣṭa; high-performance thin layer chromatography; hydrogen peroxide