title: Effects of detyrosinated tubulin on Na+,K+-ATPase activity and erythrocyte function in hypertensive subjects.
abstract: Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. Moreover, we report a reduced content and activity of the enzyme tubulin tyrosine ligase in erythrocytes of hypertensive subjects. Such alterations are related to changes in erythrocyte deformability. Our findings indicate that the detyrosination/tyrosination cycle of tubulin is important in regulation of NKA activity, and that abnormalities in this cycle are involved in hypertension development.
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Marina R
authors.initials: MR
authors.lastname: Amaiden
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina. Electronic address: vsantander@exa.unrc.edu.ar.
authors.forename: Verónica S
authors.initials: VS
authors.lastname: Santander
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Noelia E
authors.initials: NE
authors.lastname: Monesterolo
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Ayelen D
authors.initials: AD
authors.lastname: Nigra
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Juan F
authors.initials: JF
authors.lastname: Rivelli
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Alexis N
authors.initials: AN
authors.lastname: Campetelli
authors.affiliation: Unit of Clinical Genetics and Functional Genomics, Department of Pharmacology and Physiology, Medical School, University of Zaragoza, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Pie
authors.affiliation: Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, 5800 Córdoba, Argentina.
authors.forename: Cesar H
authors.initials: CH
authors.lastname: Casale
mesh_terms: D000328:Adult; D004907:Erythrocyte Deformability / Q000235:genetics; D004912:Erythrocytes / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D006973:Hypertension / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D014404:Tubulin / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
chemical_list: D014404:Tubulin; D014443:Tyrosine; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Detyrosinated tubulin; Erythrocyte deformability; Human erythrocytes; Hypertension; Na(+),K(+)-ATPase