title: Programmable sensors of 5-hydroxymethylcytosine.
abstract: 5-Hydroxymethylcytosine (hmC), the sixth base of the mammalian genome, is increasingly recognized as an epigenetic mark with important biological functions. We report engineered, programmable transcription-activator-like effectors (TALEs) as the first DNA-binding receptor molecules that provide direct, individual selectivities for cytosine (C), 5-methylcytosine (mC), and hmC at user-defined DNA sequences. Given the wide applicability of TALEs for programmable targeting of DNA sequences in vitro and in vivo, this provides broad perspectives for epigenetic research.
authors.affiliation: Department of Chemistry, Zukunftskolleg, and Konstanz Research School Chemical Biology, University of Konstanz , Universitätsstraße 10, 78457 Konstanz, Germany.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Kubik
authors.forename: Sabrina
authors.initials: S
authors.lastname: Batke
authors.forename: Daniel
authors.initials: D
authors.lastname: Summerer
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D044127:Epigenesis, Genetic; D005818:Genetic Engineering; D008958:Models, Molecular; D015394:Molecular Structure; D020080:Tandem Repeat Sequences; D015534:Trans-Activators / Q000737:chemistry*
chemical_list: D015534:Trans-Activators; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA