title: Specific genotypes of human papillomavirus in 125 high-grade squamous lesions and invasive cervical cancer cases from Congolese women.
abstract: BACKGROUND
  Knowledge on HPV prevalence and genotype distribution in HSIL and ICC is highly essential for the introduction of an effective vaccination program and appropriate epidemiological monitoring of viral ecology before and after vaccination in Congo. This study aimed to determine the specific-HPV genotypes in HSIL and ICC among women in southwestern Congo.
  
  
  METHODS
  125 archival paraffin-embedded biopsy collected between 2008 and 2012 and histologically diagnosed were investigated. DNA extraction was performed using the phenol/chloroform method. HPV search was performed by nested-PCR using MY09/MY11 and GP5+/GP6+ consensus primers followed by direct sequencing.
  
  
  RESULTS
  The mean age of participants was 44.3 ± 8.2 years. Overall, HPV prevalence was 89.6% (112/125) with all high-risk genotypes. HPV-DNA was detected in 81.5% (53/65) of HSIL and 98.3% (59/60) of ICC. HPV 16 the most common genotype was detected in 47.1% (25/53) of HSIL and 52.5% (31/59) of ICC. Other types identified were: HPV 33 (22.6%), HPV 18 (15%), HPV 31 (11.3%) and HPV 69 (3.7%) in HSIL, and HPV 33 (28.8%), HPV 18 (11.8%), HPV 31 (5%) and HPV 35 (1.7%) in ICC. Knowing that the ADC accounted for 6.7% (4/60) of ICC cases, HPV 18 was identified in 25% (1/4) of these cases against 75% (3/4) for HPV 16.
  
  
  CONCLUSIONS
  Our study showed that HPV 16, 33, 18 and 31 were the four most common genotypes in women with HSIL and ICC. These findings indicate that current vaccines against HPV could help to reduce the burden of cervical cancer in Congo.
authors.affiliation: Laboratoire de Virologie, Microbiologie et Qualité/ETB, Faculté des Sciences et Techniques, Université Hassan II Mohammedia, B.P. 146, Mohammedia, 20650, Maroc. anicet-2010@live.fr.|Laboratoire d'Agroalimentaire et Santé, Département de Biologie Appliquée, Faculté des Sciences et Techniques, Université Hassan 1er Settat, B.P. 577, Settat, Maroc. anicet-2010@live.fr.|Laboratoire d'Analyses Médicales et Morphologiques, Hôpital Général de Loandjili, B.P. 8122, Pointe-Noire, Congo. anicet-2010@live.fr.
authors.forename: Luc Magloire Anicet
authors.initials: LM
authors.lastname: Boumba
authors.affiliation: Laboratoire d'Agroalimentaire et Santé, Département de Biologie Appliquée, Faculté des Sciences et Techniques, Université Hassan 1er Settat, B.P. 577, Settat, Maroc. h_lahoucine@yahoo.fr.
authors.forename: Lahoucine
authors.initials: L
authors.lastname: Hilali
authors.affiliation: Laboratoire de Virologie, Microbiologie et Qualité/ETB, Faculté des Sciences et Techniques, Université Hassan II Mohammedia, B.P. 146, Mohammedia, 20650, Maroc. m.mouallif@doct.ulg.ac.be.|Institut Supérieur des Sciences de la santé, Université Hassan 1er Settat, B.P. 577, Settat, Maroc. m.mouallif@doct.ulg.ac.be.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Mouallif
authors.affiliation: Laboratoire d'Analyses Médicales et Morphologiques, Hôpital Général de Loandjili, B.P. 8122, Pointe-Noire, Congo. donatienmoukassa@gmail.com.
authors.forename: Donatien
authors.initials: D
authors.lastname: Moukassa
authors.affiliation: Laboratoire de Virologie, Microbiologie et Qualité/ETB, Faculté des Sciences et Techniques, Université Hassan II Mohammedia, B.P. 146, Mohammedia, 20650, Maroc. m.ennaji@yahoo.fr.
authors.forename: Moulay Mustapha
authors.initials: MM
authors.lastname: Ennaji
mesh_terms: D000328:Adult; D000368:Aged; D003223:Congo / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology* / Q000821:virology; D014932:Women's Health Services; D002578:Uterine Cervical Dysplasia / Q000453:epidemiology* / Q000821:virology