title: Label-free biomedical imaging of lipids by stimulated Raman scattering microscopy.
abstract: Advances in modern optical microscopy have provided unparalleled tools to study intracellular structure and function, yet visualizing lipid molecules within a cell remains challenging. Stimulated Raman Scattering (SRS) microscopy is a recently developed imaging modality that addresses this challenge. By selectively imaging the vibration of chemical moieties enriched in lipids, this technique allows for rapid imaging of lipid molecules in vivo without the need for perturbative extrinsic labels. SRS microscopy has been effectively employed in the study of fat metabolism, helping uncover novel regulators of lipid storage. This unit provides a brief introduction to the principle of SRS microscopy, and describes methods for its use in imaging lipids in cells, tissues, and whole organisms.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.|Medical Scientist Training Program, Baylor College of Medicine, Houston, Texas.|These authors contributed equally to this work.
authors.forename: Prasanna V
authors.initials: PV
authors.lastname: Ramachandran
authors.affiliation: Program in Developmental Biology, Baylor College of Medicine, Houston, Texas.|These authors contributed equally to this work.
authors.forename: Ayse Sena
authors.initials: AS
authors.lastname: Mutlu
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.|Program in Developmental Biology, Baylor College of Medicine, Houston, Texas.|Huffington Center on Aging, Baylor College of Medicine, Houston, Texas.|Corresponding author (wmeng@bcm.edu).
authors.forename: Meng C
authors.initials: MC
authors.lastname: Wang
mesh_terms: D008055:Lipids / Q000032:analysis*; D008853:Microscopy / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D008055:Lipids
keywords: C. elegans; cell culture; lipid; lipid droplets; metabolism; microscopy; stimulated Raman scattering; tissue section