title: Changes in cell wall properties coincide with overexpression of extensin fusion proteins in suspension cultured tobacco cells.
abstract: Extensins are one subfamily of the cell wall hydroxyproline-rich glycoproteins, containing characteristic SerHyp4 glycosylation motifs and intermolecular cross-linking motifs such as the TyrXaaTyr sequence. Extensins are believed to form a cross-linked network in the plant cell wall through the tyrosine-derivatives isodityrosine, pulcherosine, and di-isodityrosine. Overexpression of three synthetic genes encoding different elastin-arabinogalactan protein-extensin hybrids in tobacco suspension cultured cells yielded novel cross-linking glycoproteins that shared features of the extensins, arabinogalactan proteins and elastin. The cell wall properties of the three transgenic cell lines were all changed, but in different ways. One transgenic cell line showed decreased cellulose crystallinity and increased wall xyloglucan content; the second transgenic cell line contained dramatically increased hydration capacity and notably increased cell wall biomass, increased di-isodityrosine, and increased protein content; the third transgenic cell line displayed wall phenotypes similar to wild type cells, except changed xyloglucan epitope extractability. These data indicate that overexpression of modified extensins may be a route to engineer plants for bioenergy and biomaterial production.
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America; BioEnergy Science Center, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.
authors.forename: Yunqiao
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America; BioEnergy Science Center, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Pattathil
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America; BioEnergy Science Center, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Utku
authors.initials: U
authors.lastname: Avci
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America; BioEnergy Science Center, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.affiliation: Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio, United States of America.
authors.forename: Allison
authors.initials: A
authors.lastname: Arter
authors.affiliation: Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio, United States of America; Suzhou Institute of Nano-Tech and Nano-Bionics (SINANO), Suzhou, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, United States of America; BioEnergy Science Center, University of Georgia, Athens, Georgia, United States of America; Department of Plant Biology, University of Georgia, Athens, Georgia, United States of America.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Hahn
authors.affiliation: Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, United States of America.
authors.forename: Arthur J
authors.initials: AJ
authors.lastname: Ragauskas
authors.affiliation: Department of Chemistry and Biochemistry, Ohio University, Athens, Ohio, United States of America.
authors.forename: Marcia J
authors.initials: MJ
authors.lastname: Kieliszewski
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D006031:Glycosylation; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000737:chemistry / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D006023:Glycoproteins; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins; C005488:extensin protein, plant