title: A terD domain-encoding gene (SCO2368) is involved in calcium homeostasis and participates in calcium regulation of a DosR-like regulon in Streptomyces coelicolor.
abstract: Although Streptomyces coelicolor is not resistant to tellurite, it possesses several TerD domain-encoding (tdd) genes of unknown function. To elucidate the function of tdd8, the transcriptomes of S. coelicolor strain M145 and of a tdd8 deletion mutant derivative (the Δtdd8 strain) were compared. Several orthologs of Mycobacterium tuberculosis genes involved in dormancy survival were upregulated in the deletion mutant at the visual onset of prodiginine production. These genes are organized in a putative redox stress response cluster comprising two large loci. A binding motif similar to the dormancy survival regulator (DosR) binding site of M. tuberculosis has been identified in the upstream sequences of most genes in these loci. A predicted role for these genes in the redox stress response is supported by the low NAD(+)/NADH ratio in the Δtdd8 strain. This S. coelicolor gene cluster was shown to be induced by hypoxia and NO stress. While the tdd8 deletion mutant (the Δtdd8 strain) was unable to maintain calcium homeostasis in a calcium-depleted medium, the addition of Ca(2+) in Δtdd8 culture medium reduced the expression of several genes of the redox stress response cluster. The results shown in this work are consistent with Tdd8 playing a significant role in calcium homeostasis and redox stress adaptation.
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Quebec, Canada.
authors.forename: François
authors.initials: F
authors.lastname: Daigle
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Quebec, Canada.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lerat
authors.affiliation: Department of Microbial and Cellular Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom.
authors.forename: Giselda
authors.initials: G
authors.lastname: Bucca
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Quebec, Canada.
authors.forename: Édith
authors.initials: É
authors.lastname: Sanssouci
authors.affiliation: Department of Microbial and Cellular Sciences, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom.
authors.forename: Colin P
authors.initials: CP
authors.lastname: Smith
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Quebec, Canada.
authors.forename: François
authors.initials: F
authors.lastname: Malouin
authors.affiliation: Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Quebec, Canada Carole.Beaulieu@USherbrooke.ca.
authors.forename: Carole
authors.initials: C
authors.lastname: Beaulieu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D018085:Regulon; D048372:Streptomyces coelicolor / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002118:Calcium