title: Spectral overlap-free quantum dot-based determination of benzo[a]pyrene-induced cancer stem cells by concurrent monitoring of CD44, CD24 and aldehyde dehydrogenase 1.
abstract: In this study, it was found that breast cancer stem cells (CSCs) are formed from MCF-7 cells by benzo[a]pyrene (BP)-induced mutation. The breast CSCs were detected through simultaneous monitoring of CD44, CD24 and aldehyde dehydrogenase 1 (ALDH1) by hypermulticolor cellular imaging using an acousto-optical tunable filter (AOTF) and quantum dots (Q-dots).
authors.affiliation: College of Pharmacy, Seoul National University, Seoul 151-741, South Korea. jmsong@snu.ac.kr.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Shim
authors.forename: Joon Myong
authors.initials: JM
authors.lastname: Song
mesh_terms: D000080924:Aldehyde Dehydrogenase 1 Family; D001564:Benzo(a)pyrene / Q000737:chemistry* / Q000633:toxicity*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D051927:CD24 Antigen / Q000378:metabolism; D002273:Carcinogens / Q000737:chemistry / Q000633:toxicity; D002469:Cell Separation; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D061986:MCF-7 Cells; D057054:Molecular Imaging; D009154:Mutation / Q000187:drug effects; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D045663:Quantum Dots / Q000737:chemistry*; D050697:Retinal Dehydrogenase / Q000378:metabolism; D013057:Spectrum Analysis
chemical_list: D014408:Biomarkers, Tumor; D051927:CD24 Antigen; D002273:Carcinogens; D018960:Hyaluronan Receptors; D007527:Isoenzymes; D001564:Benzo(a)pyrene; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase