title: Bioanalytical method for in vitro metabolism study of repaglinide using 96-blade thin-film solid-phase microextraction and LC-MS/MS.
abstract: BACKGROUND
  A high-throughput bioanalytical method using 96-blade thin film microextraction (TFME) and LC-MS/MS for the analysis of repaglinide (RPG) and two of its main metabolites was developed and used for an in vitro metabolism study.
  
  
  RESULTS
  The target analytes were extracted from human microsomal medium by a 96-blade-TFME system employing the low-cost prototype 'SPME multi-sampler' using C18 coating. Method validation showed recoveries around 90% for all analytes and was linear over the concentration range of 2-1000 ng ml(-1) for RPG and of 2-500 ng ml(-1) for each RPG metabolite.
  
  
  CONCLUSIONS
  The method was applied to an in vitro metabolism study of RPG employing human liver microsomes and proved to be very useful for this purpose.
authors.affiliation: Núcleo de Farmácia, Universidade Federal de Sergipe, Lagarto, SE, Brazil.
authors.forename: Rodrigo Almeida
authors.initials: RA
authors.lastname: Simões
authors.forename: Pierina Sueli
authors.initials: PS
authors.lastname: Bonato
authors.forename: Fatemeh S
authors.initials: FS
authors.lastname: Mirnaghi
authors.forename: Barbara
authors.initials: B
authors.lastname: Bojko
authors.forename: Janusz
authors.initials: J
authors.lastname: Pawliszyn
mesh_terms: D002219:Carbamates / Q000008:administration & dosage / Q000627:therapeutic use*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D066298:In Vitro Techniques / Q000379:methods*; D010880:Piperidines / Q000008:administration & dosage / Q000627:therapeutic use*; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002219:Carbamates; D007004:Hypoglycemic Agents; D010880:Piperidines; C072379:repaglinide