title: [The reagents kit to detect Metyorchis biis, Opisthorchis viverrini and Clonorchis sinensis, Opisthorchis felineus--agents of opisthorchiasis using technique of polymerase chain reaction in real-time].
abstract: The helminths Opisthorchis felineus, Opisthorchis viverrini, Clonorchis sinensis, Metorchis bilis are the agents of opisthorchiasis. The actual diagnostic of parasitic diseases based on microscope analysis of samples of human feces to detect presence of ova of parasites suffers of many shortcomings, in particular low sensitivity especially at earlier stages. The purpose of this study was to compare results of detection of parasites using both classical technique and technique of specific differentiation based on extraction of nucleic acids from samples of human feces and implementation of reaction of amplification of the chosen fragment of DNA with detection of products of polymerase chain reaction in the real time. The study detected 150 out of 165 positive samples and also 6 out of 37 negative samples both validated by coproovoscopy.
authors.forename: T A
authors.initials: TA
authors.lastname: Seredina
authors.forename: V A
authors.initials: VA
authors.lastname: Petrenko
authors.forename: A V
authors.initials: AV
authors.lastname: Tronin
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Sazonov
authors.forename: O B
authors.initials: OB
authors.lastname: Sapugol'tseva
authors.forename: A V
authors.initials: AV
authors.lastname: Katokhin
authors.forename: E S
authors.initials: ES
authors.lastname: Odintsova
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002648:Child; D003004:Clonorchis sinensis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009889:Opisthorchiasis / Q000175:diagnosis* / Q000235:genetics / Q000469:parasitology; D009891:Opisthorchis / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D011933:Reagent Kits, Diagnostic
chemical_list: D004272:DNA, Mitochondrial; D011933:Reagent Kits, Diagnostic