title: Characterization of a halophilic heterotrophic nitrification-aerobic denitrification bacterium and its application on treatment of saline wastewater.
abstract: A novel halophilic bacterium capable of heterotrophic nitrification-aerobic denitrification was isolated from marine sediments and identified as Vibrio diabolicus SF16. It had ability to remove 91.82% of NH4(+)-N (119.77 mg/L) and 99.71% of NO3(-)-N (136.43 mg/L). The nitrogen balance showed that 35.83% of initial NH4(+)-N (119.77 mg/L) was changed to intracellular nitrogen, and 53.98% of the initial NH4(+)-N was converted to gaseous denitrification products. The existence of napA gene further proved the aerobic denitrification ability of strain SF16. The optimum culture conditions were salinity 1-5%, sodium acetate as carbon source, C/N 10, and pH 7.5-9.5. When an aerated biological filter system inoculated with strain SF16 was employed to treat saline wastewater, the average removal efficiency of NH4(+)-N and TN reached 97.14% and 73.92%, respectively, indicating great potential of strain SF16 for future full-scale applications.
authors.affiliation: Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China. Electronic address: duanjinming@jmu.edu.cn.
authors.forename: Jinming
authors.initials: J
authors.lastname: Duan
authors.affiliation: Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Fang
authors.affiliation: School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Su
authors.affiliation: Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Research Center of Water Treatment Engineering, Jimei University, Xiamen 361021, PR China; School of Food and Biological Engineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Lin
mesh_terms: D000332:Aerobiosis; D064751:Ammonium Compounds / Q000302:isolation & purification; D001483:Base Sequence; D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D058440:Denitrification; D005798:Genes, Bacterial; D052836:Heterotrophic Processes; D006863:Hydrogen-Ion Concentration; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification; D010802:Phylogeny; D054712:Salinity; D014733:Vibrio / Q000235:genetics / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology*; D018508:Water Purification / Q000379:methods*
chemical_list: D064751:Ammonium Compounds; D002244:Carbon; D004275:DNA, Ribosomal; D009584:Nitrogen; D062065:Wastewater
keywords: Aerated biological filter; Aerobic denitrification; Halophilic bacterium; Heterotrophic nitrification; Vibrio diabolicus