title: Studies on the role of goat heart galectin-1 as a tool for detecting post-malignant changes in glycosylation pattern.
abstract: Galectins are mammalian lectins established to play a crucial role in the progression of various cancer types by the virtue of their differential expression in normal and cancerous cells. In the present study, goat heart galectin-1 (GHG-1) was purified and investigated for its potential role in the detection of post-malignant changes in glycosylation pattern. When exposed to superoxide radicals generated from a pyrogallol auto-oxidation system, GHG-1 treated erythrocyte suspension released higher amount of oxyhemoglobin than the unagglutinated erythrocytes. The extent of erythrocyte hemolysis was found to be directly proportional to concentrations of hypochlorous acid. GHG-1 was used to detect the change in the β-galactoside expression pattern in erythrocyte membrane from human donors suffering from prostate and breast cancer. No significant change was observed in the hemolysis of lectin agglutinated erythrocytes collected from pre-operated breast cancer patients, whereas significant increase was observed in normal healthy control and post-operated samples. Findings of this study proclaim GHG-1 as an important tool for the detection of post-malignant changes in glycosylation pattern.
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Ghulam Md
authors.initials: GM
authors.lastname: Ashraf
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
authors.forename: Asma
authors.initials: A
authors.lastname: Perveen
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Shams
authors.initials: S
authors.lastname: Tabrez
authors.affiliation: Center of Excellence in Genomic Medicine Research, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Zaidi
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Kamal
authors.affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India ; College of Medical Rehabilitation, Qassim University, Buraydah, Saudi Arabia.
authors.forename: Naheed
authors.initials: N
authors.lastname: Banu
keywords: Cancer; GHG-1, goat heart galectin-1; Gal-1, galectin-1; Glycosylation; Goat heart galectin-1; HOCl; HOCl, hypochlorous acid; OxyHb, oxyhemoglobin; Oxyhemoglobin; Pyrogallol