title: Functionalization of alkyne-terminated thermally hydrocarbonized porous silicon nanoparticles with targeting peptides and antifouling polymers: effect on the human plasma protein adsorption.
abstract: Porous silicon (PSi) nanomaterials combine a high drug loading capacity and tunable surface chemistry with various surface modifications to meet the requirements for biomedical applications. In this work, alkyne-terminated thermally hydrocarbonized porous silicon (THCPSi) nanoparticles were fabricated and postmodified using five bioactive molecules (targeting peptides and antifouling polymers) via a single-step click chemistry to modulate the bioactivity of the THCPSi nanoparticles, such as enhancing the cellular uptake and reducing the plasma protein association. The size of the nanoparticles after modification was increased from 176 to 180-220 nm. Dextran 40 kDa modified THCPSi nanoparticles showed the highest stability in aqueous buffer. Both peptide- and polymer-functionalized THCPSi nanoparticles showed an extensive cellular uptake which was dependent on the functionalized moieties presented on the surface of the nanoparticles. The plasma protein adsorption study showed that the surface modification with different peptides or polymers induced different protein association profiles. Dextran 40 kDa functionalized THCPSi nanoparticles presented the least protein association. Overall, these results demonstrate that the "click" conjugation of the biomolecules onto the alkyne-terminated THCPSi nanoparticles is a versatile and simple approach to modulate the surface chemistry, which has high potential for biomedical applications.
authors.affiliation: Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki , FI-00014 Helsinki, Finland.
authors.forename: Chang-Fang
authors.initials: CF
authors.lastname: Wang
authors.forename: Ermei M
authors.initials: EM
authors.lastname: Mäkilä
authors.forename: Colin
authors.initials: C
authors.lastname: Bonduelle
authors.forename: Jussi
authors.initials: J
authors.lastname: Rytkönen
authors.forename: Janne
authors.initials: J
authors.lastname: Raula
authors.forename: Sérgio
authors.initials: S
authors.lastname: Almeida
authors.forename: Ale
authors.initials: A
authors.lastname: Närvänen
authors.forename: Jarno J
authors.initials: JJ
authors.lastname: Salonen
authors.forename: Sebastien
authors.initials: S
authors.lastname: Lecommandoux
authors.forename: Jouni T
authors.initials: JT
authors.lastname: Hirvonen
authors.forename: Hélder A
authors.initials: HA
authors.lastname: Santos
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D001798:Blood Proteins / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D016062:Porosity; D012825:Silicon / Q000737:chemistry*
chemical_list: D000480:Alkynes; D001798:Blood Proteins; D004337:Drug Carriers; D010455:Peptides; D011108:Polymers; D012825:Silicon
keywords: cell−nanoparticle interaction; click chemistry; porous silicon; protein adsorption; surface modification