title: Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex.
abstract: The synthesis of a new pyrene-containing Fischer carbene complex is described. The complex has a broad absorbance spectrum between 300 and 400 nm and, on excitation at 345 nm in CH2Cl2 solution, emission is observed at 395 and 415 nm. Emission is also observed in PBS buffer, but in this case the resulting spectra are much broader. Confocal and fluorescence lifetime imaging indicate that emission occurs on treating HeLa cells with the complex and co-localisation studies demonstrate that this is from the mitochondria and lipid-rich regions of the cell.
authors.affiliation: Department of Chemistry, University of Bath, Claverton Down, Bath, BA2 7AY, UK. S.Pascu@bath.ac.uk.
authors.forename: Rory L
authors.initials: RL
authors.lastname: Arrowsmith
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Atkin
authors.forename: Stanley W
authors.initials: SW
authors.lastname: Botchway
authors.forename: Ian J S
authors.initials: IJ
authors.lastname: Fairlamb
authors.forename: Jason M
authors.initials: JM
authors.lastname: Lynam
authors.forename: James W B
authors.initials: JW
authors.lastname: Moir
authors.forename: Sofia I
authors.initials: SI
authors.lastname: Pascu
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Ward
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Zhang
mesh_terms: D001692:Biological Transport; D002248:Carbon Monoxide / Q000737:chemistry*; D002857:Chromium / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D018613:Microscopy, Confocal / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry*
chemical_list: D009942:Organometallic Compounds; D011721:Pyrenes; D002857:Chromium; C030011:carbene; D002248:Carbon Monoxide; C030984:pyrene; D008697:Methane