title: Prevalence of blaIMP, and blaVIM gene carriage in metallo-β-lactamase-producing burn isolates of Pseudomonas aeruginosa in Tehran.
abstract: OBJECTIVE
  To study the prevalence of blaVIM and blaIMP genes in metallo-β-lactamase (MBL)-producing burn isolates of Pseudomonas aeruginosa in relation with AmpC and extended-spectrum β-lactamase (ESBL) production.
  
  
  METHODS
  Thirty-two carbapenem-resistant MBL-producing P aeruginosa burn isolates from Shahid Motahari Burn Hospital in Tehran were employed. Antibiotic susceptibility was determined to 13 antibiotics including imipenem and meropenem by disk diffusion. AmpC and ESBL production was detected by the AmpC disk test and combined disk diffusion assay, respectively, blaIMP and blaVIM gene carriage was shown by polymerase chain reaction and type-specific primers.
  
  
  RESULTS
  AmpC production was observed in 81% and ESBL production was detected in 12.5% of the isolates. blalMP carriage was observed in 56.25% and blaVIM gene in 46.8% of the isolates. Surprisingly, 43.5% of the isolates carried both blalMP and blaviM genes.
  
  
  CONCLUSIONS
  We think that this is the first report on the cocarriage of blalMP and blavIM in P aeruginosa. There was also a strong association between MBL gene carriage and AmpC β-lactamase production.
authors.affiliation: Department of Microbiology, Shahid Beheshti University, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Salimi
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Eftekhar
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002056:Burns / Q000382:microbiology*; D015780:Carbapenems / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases