title: Antibacterial activity and mechanism of action of Monarda punctata essential oil and its main components against common bacterial pathogens in respiratory tract.
abstract: The aim of the current research work was to study the chemical composition of the essential oil of Monarda punctata along with evaluating the essential oil and its major components for their antibacterial effects against some frequently encountered respiratory infection causing pathogens. Gas chromatographic mass spectrometric analysis revealed the presence of 13 chemical constituents with thymol (75.2%), p-cymene (6.7%), limonene (5.4), and carvacrol (3.5%) as the major constituents. The oil composition was dominated by the oxygenated monoterpenes. Antibacterial activity of the essential oil and its major constituents (thymol, p-cymene, limonene) was evaluated against Streptococcus pyogenes, methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus pneumoniae, Haemophilus influenzae and Escherichia coli. The study revealed that the essential oil and its constituents exhibited a broad spectrum and variable degree of antibacterial activity against different strains. Among the tested strains, Streptococcus pyogenes, Escherichia coli and Streptococcus pneumoniae were the most susceptible bacterial strain showing lowest MIC and MBC values. Methicillin-resistant Staphylococcus aureus was the most resistant bacterial strain to the essential oil treatment showing relatively higher MIC and MBC values. Scanning electron microscopy revealed that the essential oil induced potent and dose-dependent membrane damage in S. pyogenes and MRSA bacterial strains. The reactive oxygen species generated by the Monarda punctata essential oil were identified using 2', 7'-dichlorofluorescein diacetate (DCFDA).This study indicated that the Monarda punctata essential oil to a great extent and thymol to a lower extent triggered a substantial increase in the ROS levels in S. pyogenes bacterial cultures which ultimately cause membrane damage as revealed by SEM results.
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiantong University Xi'an, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiantong University Xi'an, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiantong University Xi'an, China.
authors.forename: Fei-Yan
authors.initials: FY
authors.lastname: Li
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiantong University Xi'an, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Xi'an Jiantong University Xi'an, China.
authors.forename: Zhong-Min
authors.initials: ZM
authors.lastname: Sun
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D053138:Cyclohexenes / Q000494:pharmacology; D000081007:Cymenes; D004926:Escherichia coli / Q000187:drug effects; D000077222:Limonene; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D031338:Monarda / Q000737:chemistry*; D039821:Monoterpenes / Q000494:pharmacology; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology*; D012137:Respiratory System / Q000382:microbiology; D013296:Streptococcus pneumoniae / Q000187:drug effects*; D013297:Streptococcus pyogenes / Q000187:drug effects*; D013729:Terpenes / Q000494:pharmacology; D013943:Thymol / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D053138:Cyclohexenes; D000081007:Cymenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; C007210:4-cymene; D013943:Thymol; C073316:carvacrol; D000077222:Limonene
keywords: Monarda punctata; Streptococcus pyogenes; essential oil; methicillin-resistant Staphylococcus aureus; p-cymene; thymol