title: Live CLEM imaging to analyze nuclear structures at high resolution.
abstract: Fluorescence microscopy (FM) and electron microscopy (EM) are powerful tools for observing molecular components in cells. FM can provide temporal information about cellular proteins and structures in living cells. EM provides nanometer resolution images of cellular structures in fixed cells. We have combined FM and EM to develop a new method of correlative light and electron microscopy (CLEM), called "Live CLEM." In this method, the dynamic behavior of specific molecules of interest is first observed in living cells using fluorescence microscopy (FM) and then cellular structures in the same cell are observed using electron microscopy (EM). Following image acquisition, FM and EM images are compared to enable the fluorescent images to be correlated with the high-resolution images of cellular structures obtained using EM. As this method enables analysis of dynamic events involving specific molecules of interest in the context of specific cellular structures at high resolution, it is useful for the study of nuclear structures including nuclear bodies. Here we describe Live CLEM that can be applied to the study of nuclear structures in mammalian cells.
authors.affiliation: Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe, 651-2492, Japan, tokuko@nict.go.jp.
authors.forename: Tokuko
authors.initials: T
authors.lastname: Haraguchi
authors.forename: Hiroko
authors.initials: H
authors.lastname: Osakada
authors.forename: Takako
authors.initials: T
authors.lastname: Koujin
mesh_terms: D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D045586:Intranuclear Inclusion Bodies / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*