title: Preparation of the oligosaccharides derived from Flammulina velutipes and their antioxidant activities.
abstract: The oligosaccharides were prepared from Flammulina velutipes by hydrolysis of F. velutipes polysaccharides with hydrogen peroxide (H2O2). The yields of F. velutipes derived oligosaccharides (FVOs) were monitored during the hydrolysis process. FVOs yields were affected by three factors, i.e. reaction temperature, H2O2 concentration, and time, which were optimized by using an orthogonal design experiments as follows: reaction temperature 70°C, H2O2 concentration 3%, and reaction time 6h. Under these optimum conditions, the maximal yield of the oligosaccharides reached 17.10%, which was higher than that of hot water extraction method. The oligosaccharides were partially characterized by Fourier transform infrared spectrum, monosaccharide composition, and antioxidant activity. The results indicate that the oligosaccharides derived from F. velutipes showed strong hydroxyl radical activity and reducing capacity at the concentration of 100 μg/mL.
authors.affiliation: School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China. Electronic address: xiazqhhit@126.com.
authors.forename: Zhenqiang
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D055393:Flammulina / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry*; D013696:Temperature
chemical_list: D000975:Antioxidants; D009844:Oligosaccharides; D006861:Hydrogen Peroxide
keywords: Flammulina velutipes; Hydrogen peroxide; Oligosaccharides