title: Heat shock proteins: in vivo heat treatments reveal adipose tissue depot-specific effects.
abstract: Heat treatments (HT) and the induction of heat shock proteins (HSPs) improve whole body and skeletal muscle insulin sensitivity while decreasing white adipose tissue (WAT) mass. However, HSPs in WAT have been understudied. The purpose of the present study was to examine patterns of HSP expression in WAT depots, and to examine the effects of a single in vivo HT on WAT metabolism. Male Wistar rats received HT (41°C, 20 min) or sham treatment (37°C), and 24 h later subcutaneous, epididymal, and retroperitoneal WAT depots (SCAT, eWAT, and rpWAT, respectively) were removed for ex vivo experiments and Western blotting. SCAT, eWAT, and rpWAT from a subset of rats were also cultured separately and received a single in vitro HT or sham treatment. HSP72 and HSP25 expression was greatest in more metabolically active WAT depots (i.e., eWAT and rpWAT) compared with the SCAT. Following HT, HSP72 increased in all depots with the greatest induction occurring in the SCAT. In addition, HSP25 increased in the rpWAT and eWAT, while HSP60 increased in the rpWAT only in vivo. Free fatty acid (FFA) release from WAT explants was increased following HT in the rpWAT only, and fatty acid reesterification was decreased in the rpWAT but increased in the SCAT following HT. HT increased insulin responsiveness in eWAT, but not in SCAT or rpWAT. Differences in HSP expression and induction patterns following HT further support the growing body of literature differentiating distinct WAT depots in health and disease.
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas; and.
authors.forename: Robert S
authors.initials: RS
authors.lastname: Rogers
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Marie-Soleil
authors.initials: MS
authors.lastname: Beaudoin
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas; and.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Wheatley
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.
authors.forename: David C
authors.initials: DC
authors.lastname: Wright
authors.affiliation: Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas; and pgeiger@kumc.edu.
authors.forename: Paige C
authors.initials: PC
authors.lastname: Geiger
mesh_terms: D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D050884:HSP72 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D006358:Hot Temperature; D008066:Lipolysis / Q000502:physiology; D008297:Male; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins
keywords: HSP72; fatty acid reesterification; lipolysis; metabolism; stress response