title: Mitochondria-targeted antioxidant SkQ1 reverses glaucomatous lesions in rabbits.
abstract: Glaucoma is the main cause of irreversible blindness worldwide. This disease is characterized by apoptosis of retinal ganglion cells (RGC) and visual field loss that seems to be related to elevated intraocular pressure (IOP). Several lines of evidences have implicated the crucial role of mitochondrial dysfunction in the pathogenesis of glaucoma. Increased mitochondrial oxidative stress in RGC may underlie or contribute to susceptibility of RGC to apoptosis. In our work we (i) designed a rabbit model of chronic, moderately elevated IOP for studying glaucoma and (ii) demonstrated efficacy of mitochondria-targeted antioxidant SkQ1 as a tool to reverse several traits of experimental glaucoma induced by a series of injections of hydroxypropylmethylcellulose (HPMC) to the anterior chamber of the rabbit eye. It is shown that 6 months instillations of drops of 0.2.5-5 microM solution of SkQ1 normalize IOP and eye hydrodynamics and abolish an increase in lens thickness that accompanies glaucoma.
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Iomdina
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Inna P
authors.initials: IP
authors.lastname: Khoroshilova-Maslova
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Robustova
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Averina
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Nadezhda A
authors.initials: NA
authors.lastname: Kovaleva
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Gjumrakch
authors.initials: G
authors.lastname: Aliev
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: V Prakash
authors.initials: VP
authors.lastname: Reddy
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Zamyatnin
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Maxim V
authors.initials: MV
authors.lastname: Skulachev
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Ivan I
authors.initials: II
authors.lastname: Senin
authors.affiliation: Lomonosov Moscow State University, Faculty of Bioengineering and Bioinformatics, Moscow 119992, Russia.
authors.forename: Vladimir P
authors.initials: VP
authors.lastname: Skulachev
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D005901:Glaucoma / Q000188:drug therapy* / Q000503:physiopathology; D007429:Intraocular Pressure / Q000187:drug effects; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D010971:Plastoquinone / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D011817:Rabbits
chemical_list: C532038:10-(6'-plastoquinonyl)decyltriphenylphosphonium; D000975:Antioxidants; D010971:Plastoquinone