title: IGF-1 from adipose-derived mesenchymal stem cells promotes radioresistance of breast cancer cells.
abstract: OBJECTIVE
  The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells.
  
  
  METHODS
  MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; γ-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting.
  
  
  RESULTS
  AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells.
  
  
  CONCLUSIONS
  Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.
authors.affiliation: Department of Anatomy, Nanfang Hospital, Southern Medical University, Guangzhou, China E-mail : daijingxing@gmail.com; ylsmuanatomy@163.com.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Yang
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Qu
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Lin
authors.forename: Tong-Xin
authors.initials: TX
authors.lastname: Liu
authors.forename: Quan-Quan
authors.initials: QQ
authors.lastname: Sun
authors.forename: Chun
authors.initials: C
authors.lastname: Yang
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Hu
authors.forename: Jing-Xing
authors.initials: JX
authors.lastname: Dai
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001943:Breast Neoplasms / Q000378:metabolism* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000528:radiation effects; D049109:Cell Proliferation / Q000502:physiology* / Q000528:radiation effects; D005260:Female; D006657:Histones / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D061986:MCF-7 Cells; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011827:Radiation; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000378:metabolism / Q000528:radiation effects; D059016:Tumor Microenvironment
chemical_list: C468783:H2AX protein, human; D006657:Histones; C583557:IGF1 protein, human; C000597236:IGF1R protein, human; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1