title: Effect of the treatment of focal brain ablation in rat with bone marrow mesenchymal stromal cells on sensorimotor recovery and cytokine production.
abstract: Increased efficacy of the multipotent mesenchymal stromal cells (MSCs) for the treatment of CNS injuries has been shown when they are administrated within a collagen scaffold, an environment in three dimensions (3D), when compared to the cultivation over a plastic surface (2D). We evaluated the MSC therapeutic effect in the 2D and 3D conditions using the model of focal cortical ablation. Male Wistar rats were submitted to the ablation by aspiration. Intravenous injection (IV) of MSC cultured in 2D, and the intralesional administration (IL) of MSC cultured in 2D or 3D were tested. Administrations were made 24h after ablation. Unskilled and skilled forelimb movements were evaluated by sensorimotor tests. The level of cytokines was measured two days after ablation in the 2D IV groups. Only the MSC 3D IL promoted recovery of the skilled movements. MSC 2D IV promoted recovery of the unskilled movements in all tests, and the MSC 3D IL promoted it only in the adhesive test. MSC 2D IL was unable to promote any recovery. DAPI-stained MSC was found in the perilesional parenchyma at the third post-ablation day after 2D and 3D IL. A significant reduction in the levels of cytokines by the MSC 2D IV was observed in the plasma. Our study strengthens the evidences of the MSC as a prospective therapeutic approach for the CNS injuries.
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil.
authors.forename: Helder Teixeira
authors.initials: HT
authors.lastname: de Freitas
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil.
authors.forename: Mariana Gomes
authors.initials: MG
authors.lastname: Rebel
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil.
authors.forename: Bárbara Paula
authors.initials: BP
authors.lastname: Coelho
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil.
authors.forename: Viviane Gomes
authors.initials: VG
authors.lastname: da Silva
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil.
authors.forename: Gisela Garcia Cabral
authors.initials: GG
authors.lastname: Galaxe-Almeida
authors.affiliation: Laboratório de Biologia Celular e Tecidual (Laboratory of Cell and Tissue Biology) - Centro de Biociências e Biotecnologia (Center of Biosciences and Biotechnology) - Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) (State University of North Fluminense) - Campos dos Goytacazes - RJ, Brazil. Electronic address: agiraldi@uenf.br.
authors.forename: Arthur
authors.initials: A
authors.lastname: Giraldi-Guimarães
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001853:Bone Marrow; D001930:Brain Injuries / Q000628:therapy*; D003094:Collagen; D016207:Cytokines / Q000097:blood*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D009068:Movement / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000502:physiology*
chemical_list: D016207:Cytokines; D003094:Collagen
keywords: Brain injury; Cell therapy; Cerebral cortex; Collagen scaffold; Functional recovery; Mesenchymal stem cells