title: Relationship between cardiac tissue glycation and skin autofluorescence in patients with coronary artery disease.
abstract: OBJECTIVE
  During ageing, advanced glycation end-products (AGEs) accumulate in extracellular matrix proteins like collagen and contribute to a decline in organ function. As skin autofluorescence (sAF) can assess subcutaneous accumulation of fluorescent AGEs, this study aimed to investigate the relationship between AGE-modified cardiac tissue collagen and AGE-related sAF in coronary artery bypass graft (CABG) surgery patients.
  
  
  METHODS
  Between January 2011 and January 2012, data from 72 consecutive male patients undergoing isolated CABG were prospectively recorded. Collagen fractions were isolated from the right atrial appendages of these patients by proteolysis and collagenase digestion. Collagen was quantified by hydroxyproline assay, and AGEs by AGE-related intrinsic fluorescence; sAF was measured using an autofluorescence reader.
  
  
  RESULTS
  Biochemical analysis showed that the insoluble cardiac collagen fraction contained the highest amounts of accumulated AGEs; the AGE-related intrinsic fluorescence of this fraction increased with age (P=0.0001), blood glucose (P=0.002), HbA1c (P=0.01) and sAF (P=0.008).
  
  
  CONCLUSIONS
  This study demonstrated for the first time a relationship between cardiac tissue glycation and AGE-related sAF. In addition, cardiac tissue glycation was associated with age, blood glucose and long-term glucose values in patients with coronary artery disease.
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital Halle, Halle (Saale), Germany. Electronic address: britt.hofmann@medizin.uni-halle.de.
authors.forename: B
authors.initials: B
authors.lastname: Hofmann
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital Halle, Halle (Saale), Germany.
authors.forename: K
authors.initials: K
authors.lastname: Jacobs
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital Halle, Halle (Saale), Germany.
authors.forename: A
authors.initials: A
authors.lastname: Navarrete Santos
authors.affiliation: Institute of Medical Epidemiology, Biostatistics and Informatics, Martin-Luther-University Halle-Wittenberg, Halle (Saale), Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wienke
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital Halle, Halle (Saale), Germany.
authors.forename: R E
authors.initials: RE
authors.lastname: Silber
authors.affiliation: Department of Cardiothoracic Surgery, University Hospital Halle, Halle (Saale), Germany.
authors.forename: A
authors.initials: A
authors.lastname: Simm
mesh_terms: D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D020517:Atrial Appendage / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D003094:Collagen / Q000737:chemistry / Q000378:metabolism; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000097:blood / Q000150:complications / Q000378:metabolism* / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D003925:Diabetic Angiopathies / Q000097:blood / Q000378:metabolism* / Q000601:surgery; D005858:Germany; D006442:Glycated Hemoglobin / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D008297:Male; D061848:Optical Imaging; D011446:Prospective Studies; D012867:Skin / Q000378:metabolism*; D040521:Subcutaneous Tissue / Q000378:metabolism*; D015854:Up-Regulation; D044465:White People
chemical_list: D015415:Biomarkers; D003094:Collagen; D006442:Glycated Hemoglobin; D017127:Glycation End Products, Advanced; C517652:hemoglobin A1c protein, human
keywords: AGEs; Coronary artery disease; Diabetes mellitus; Skin autofluorescence