title: Effective collection of peripheral blood stem cells in children weighing 20 kilogram or less in a single large-volume apheresis procedure.
abstract: BACKGROUND
  Peripheral blood stem cell (PBSC) transplantation has become a routine procedure in pediatric oncology. A special group of PBSC donors are children weighing 20 kg or less. Limited vascular access and low blood volume puts them at a higher risk. Central line placement and a priming apheresis machine are recommended to avoid these complications.
  
  
  METHODS
  PBSC collections performed from July 2006 to May 2013 in children weighing less than 20 kg were included. All donors had a central venous catheter (CVC). An apheresis machine was primed with packet red blood cells.
  
  
  RESULTS
  Twenty-seven PBSC collections were performed in 22 children weighing 20 kg or less, 14 for allogeneic and 8 for autologous transplantation, in order to collect at least 2 × 10(6) CD34+ cells/kg. In the allogeneic group, median age and weight were 3 years (0.8-7) and 15.5 kg (8-20). In the autologous group, median age and weight were 3 years (2-7) and 15.35 kg (12.5-19.5). A single large-volume apheresis was sufficient to obtain the CD34+ cells needed in 78.5% and 75% of the allogeneic and autologous groups, respectively, with a median 11.84 × 10(6) and 5.79 × 10(6) CD34+ cells collected per kilogram of weight of the recipient. No serious complications related to the apheresis procedure or CVC placement occurred.
  
  
  CONCLUSIONS
  PBSC collection in a single large-volume apheresis for allogeneic and autologous transplants in children weighing 20 kg or less is a safe and effective procedure when based on standardized protocols.
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Rosario
authors.initials: R
authors.lastname: Salazar-Riojas
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: José Alberto
authors.initials: JA
authors.lastname: García-Lozano
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Mayra
authors.initials: M
authors.lastname: Valdés-Galván
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Odra
authors.initials: O
authors.lastname: Martínez-González
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Olga Graciela
authors.initials: OG
authors.lastname: Cantú-Rodríguez
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Oscar
authors.initials: O
authors.lastname: González-Llano
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: Andrés
authors.initials: A
authors.lastname: Gómez-De León
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Jaime-Pérez
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: David
authors.initials: D
authors.lastname: Gómez-Almaguer
authors.affiliation: Servicio De Hematología, Hospital Universitario "Dr. José Eleuterio González," U.A.N.L., Monterrey, Nuevo Leon, México.
authors.forename: César Homero
authors.initials: CH
authors.lastname: Gutiérrez-Aguirre
mesh_terms: D064591:Allografts; D000741:Anemia, Aplastic / Q000628:therapy; D018952:Antigens, CD34 / Q000032:analysis; D001835:Body Weight; D002405:Catheterization, Central Venous / Q000379:methods; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D019343:Citric Acid; D003520:Cyclophosphamide / Q000494:pharmacology; D016238:Cytapheresis / Q000295:instrumentation / Q000379:methods*; D004912:Erythrocytes; D000069585:Filgrastim / Q000494:pharmacology; D005947:Glucose / Q000031:analogs & derivatives; D019337:Hematologic Neoplasms / Q000628:therapy; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000737:chemistry; D006801:Humans; D009447:Neuroblastoma / Q000628:therapy; D036102:Peripheral Blood Stem Cell Transplantation; D012189:Retrospective Studies; D016019:Survival Analysis; D014182:Transplantation, Autologous; D016896:Treatment Outcome
chemical_list: D018952:Antigens, CD34; C002113:acid citrate dextrose; D019343:Citric Acid; D003520:Cyclophosphamide; D005947:Glucose; D000069585:Filgrastim
keywords: allogeneic transplant; autologous transplant; cellular mobilization; low-weight children; peripheral blood stem cell collection