title: Lime application for the efficient production of nutraceutical glucooligosaccharides from Leuconostoc mesenteroides NRRL B-742 (ATCC13146).
abstract: We have previously demonstrated the production of glucooligosaccharides via a fermentation of sucrose with Leuconostoc mesenteroides NRRL B-742 using sodium hydroxide (NaOH) to control the pH. Because NaOH is expensive, we sought to minimize the cost of our process by substituting hydrated lime and saccharate of lime (lime sucrate) in its place. The yield of glucooligosaccharides using either 5 % lime (41.4 ± 0.5 g/100 g) or 5 % lime sucrate (40.0 ± 1.4 g/100 g) were both similar to the NaOH control (42.4 ± 1.5 g/100 g). Based on this, it appears that the cost associated with pH control in our process can be reduced by a factor of approximately 2.4 using lime instead of NaOH. Because our chromatographic stage is based on a Ca(2+)-form resin to separate glucooligosaccharides, the use of lime not only negates the need for costly de-salting via ion-exchange (elimination of two ion-exchange sections) prior to separation, but also greatly reduces the resin regeneration cost.
authors.affiliation: Audubon Sugar Institute, Louisiana State University Agricultural Center, 3,845 Highway 75, Saint Gabriel, LA, 70776, USA, ymoon@agcenter.lsu.edu.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Moon
authors.forename: Lee
authors.initials: L
authors.lastname: Madsen
authors.forename: Chang-Ho
authors.initials: CH
authors.lastname: Chung
authors.forename: Doman
authors.initials: D
authors.lastname: Kim
authors.forename: Donal F
authors.initials: DF
authors.lastname: Day
mesh_terms: D017610:Calcium Compounds / Q000737:chemistry*; D003470:Culture Media; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry; D007934:Leuconostoc / Q000378:metabolism*; D008353:Mannitol / Q000737:chemistry; D009844:Oligosaccharides / Q000096:biosynthesis*; D010087:Oxides / Q000737:chemistry*; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D017610:Calcium Compounds; D003470:Culture Media; D009844:Oligosaccharides; D010087:Oxides; D019344:Lactic Acid; D008353:Mannitol; D012972:Sodium Hydroxide; C016538:lime