title: Reduction in voluntary ethanol intake following repeated oral administration of Jodina rhombifolia lyophilized aqueous extract in male Wistar rats.
abstract: BACKGROUND
  The leaves of Jodina rhombifolia (Hook. & Arn.) Reissek (SANTALACEAE) is utilized in Argentine folk medicine for the treatment of alcoholism.
  
  
  OBJECTIVE
  To evaluate the antialcoholic activity of Jodina rhombifolia lyophilized aqueous extract (JRLE) in male Wistar rats.
  
  
  METHODS
  Rats were housed individually in standard plastic cages with wood chip bedding. Throughout the duration of experiment, ethanol was offered in the standard home-cage; two-bottle free-choice regimen between an ethanolic solution (20% in tap water, v/v) and tap water with unlimited access for 24h per day for 10 consecutive days. Rats were administrated intragastrically twice daily (1 ml/200 g) for 10 consecutive days, with the control vehicle (distilled water) or one of the doses of JRLE (125, 250 and 500 mg/Kg weight). Body weight, ethanol, water and food intake were measured every day at the same hour during the 10 days of experimentation.
  
  
  RESULTS
  The reducing effect of JRLE on daily ethanol intake was evidenced from the first day of treatment and persisted throughout the entire treatment period. The treatment did not significantly affect daily water intake neither the body weight gain. Daily food intake was higher in rat groups treated with JRLE.
  
  
  CONCLUSIONS
  The results obtained in the present preliminary study show that repeated administration of JRLE, markedly reduces ethanol voluntary intake in male Wistar rats. The reduction of consumption was of remarkable magnitude and stable during the treatment 10-days.
authors.affiliation: Farmacología, Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina. Electronic address: maurote@unsl.edu.ar.
authors.forename: Mauricio Roberto
authors.initials: MR
authors.lastname: Teves
authors.affiliation: Farmacología, Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina.
authors.forename: Graciela Haydée
authors.initials: GH
authors.lastname: Wendel
authors.affiliation: Farmacología, Departamento de Farmacia, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Chacabuco y Pedernera, 5700 San Luis, Argentina.
authors.forename: Lilian Eugenia
authors.initials: LE
authors.lastname: Pelzer
mesh_terms: D000427:Alcohol Deterrents / Q000494:pharmacology*; D000428:Alcohol Drinking / Q000188:drug therapy*; D000818:Animals; D004326:Drinking; D004435:Eating; D005612:Freeze Drying; D019684:Magnoliopsida; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D017208:Rats, Wistar
chemical_list: D000427:Alcohol Deterrents; D010936:Plant Extracts
keywords: Antialcoholic; Jodina rhombifolia; Male Wistar rats; Two-bottle choice; Voluntary ethanol intake