title: Directed interactions of block copolypept(o)ides with mannose-binding receptors: PeptoMicelles targeted to cells of the innate immune system.
abstract: Core-shell structures based on polypept(o)ides combine stealth-like properties of the corona material polysarcosine with adjustable functionalities of the polypeptidic core. Mannose-bearing block copolypept(o)ides (PSar-block-PGlu(OBn)) have been synthesized using 11-amino-3,6,9-trioxa-undecyl-2,3,4,6-tetra-O-acetyl-O-α-D-mannopyranoside as initiator in the sequential ring-opening polymerization of α-amino acid N-carboxyanhydrides. These amphiphilic block copolypept(o)ides self-assemble into multivalent PeptoMicelles and bind to mannose-binding receptors as expressed by dendritic cells. Mannosylated micelles showed enhanced cell uptake in DC 2.4 cells and in bone marrow-derived dendritic cells (BMDCs) and therefore appear to be a suitable platform for immune modulation.
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University, Duesbergweg 10-14, 55099, Mainz, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Heller
authors.forename: Nicole
authors.initials: N
authors.lastname: Mohr
authors.forename: Alexander
authors.initials: A
authors.lastname: Birke
authors.forename: Benjamin
authors.initials: B
authors.lastname: Weber
authors.forename: Angelika
authors.initials: A
authors.lastname: Reske-Kunz
authors.forename: Matthias
authors.initials: M
authors.lastname: Bros
authors.forename: Matthias
authors.initials: M
authors.lastname: Barz
mesh_terms: D000812:Anhydrides / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003713:Dendritic Cells / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods; D007113:Immunity, Innate / Q000502:physiology*; D056747:Immunomodulation / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy; D037601:Mannose-Binding Lectin / Q000378:metabolism*; D051379:Mice; D008823:Micelles; D050997:Nanomedicine / Q000379:methods / Q000639:trends; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D000812:Anhydrides; D037601:Mannose-Binding Lectin; D008823:Micelles; D010455:Peptides; D013778:Tetrazolium Salts; D013844:Thiazoles; C015475:polysarcosine; C022616:thiazolyl blue; D012521:Sarcosine
keywords: mannose; micelles; polypeptides; polysarcosine; targeting