title: Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters.
abstract: OBJECTIVE
  To evaluate the effects of montelukast and Hypericum perforatum against ischemia/reperfusion (I/R)-induced intestinal damage.
  
  
  METHODS
  Twenty-eight hamsters were divided into 4 groups following midline abdominal laparotomy: control group (n = 7), I/R group (n = 7), montelukast and I/R (MIR) group (n = 7), and Hypericum perforatum and I/R (HPIR) group (n = 7). After 60 min of ischemia through obstruction of the superior mesenteric artery, 24 h of reperfusion was maintained. Ten minutes prior to the reperfusion period, the MIR group received 7 mg/kg of intraperitoneal montelukast and the HPIR group received 7 mg/kg of intraperitoneal Hypericum perforatum. Malondialdehyde, glutathione, myeloperoxidase, and cardiotrophin-1 levels were measured from blood samples. A semiquantitative histological evaluation was performed.
  
  
  RESULTS
  Montelukast and Hypericum perforatum significantly reduced malondialdehyde levels and increased glutathione levels compared to the I/R group (P < 0.008). A statistically significant difference was also found between the I/R group and MIR and HPIR groups in terms of myelqperoxidase levels (P < 0.008). The MIR and HPIR groups showed increased cardiotrophin- 1 levels compared to the control and I/R groups (P < 0.008 for all). The MIR and HPIR groups showed significantly lower histological scores compared to the I/R group (P = 0.03 and P = 0.007, respectively).
  
  
  CONCLUSIONS
  This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.
authors.affiliation: Department of Emergency Medicine, Abant İzzet Baysal University, Bolu, Turkey. tarikocak78@gmail.com
authors.forename: Tank
authors.initials: T
authors.lastname: Ocak
authors.forename: Arif
authors.initials: A
authors.lastname: Duran
authors.forename: Gülzade
authors.initials: G
authors.lastname: Özyalvaçli
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Ocak
authors.forename: Elçin Hakan
authors.initials: EH
authors.lastname: Terzi
authors.forename: Mehmet
authors.initials: M
authors.lastname: Tosuns
authors.forename: Kemalettin
authors.initials: K
authors.lastname: Erdem
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D006224:Cricetinae; D003521:Cyclopropanes; D004195:Disease Models, Animal; D005978:Glutathione / Q000097:blood; D020902:Hypericum / Q000737:chemistry*; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood; D017538:Mesenteric Artery, Superior / Q000293:injuries; D008647:Mesocricetus; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D011804:Quinolines / Q000494:pharmacology*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy* / Q000517:prevention & control*; D013440:Sulfides
chemical_list: D000085:Acetates; D003521:Cyclopropanes; D010936:Plant Extracts; D020011:Protective Agents; D011804:Quinolines; D013440:Sulfides; D008315:Malondialdehyde; D005978:Glutathione; C093875:montelukast