title: Nlrx1 regulates neuronal cell death.
abstract: BACKGROUND
  Regulation of cell death during neurodegeneration is one of the key factors that play a role in the speed at which a disease progresses. Out of several cellular pathways responsible for this progression, necrosis and apoptosis are situated on the opposite spectrum of cell death regulation. Necrosis produces an environment that promotes inflammation and cytotoxicity and apoptosis is a highly organized process that maintains tissue homeostasis. A recently discovered protein, Nlrx1, regulates inflammatory and cell death responses during infection.
  
  
  RESULTS
  Using transfections of N2A cell line, we demonstrate that Nlrx1 redirects cells away from necrosis and towards an apoptotic pathway following rotenone treatments. In addition, Nlrx1 promotes DRP1 phosphorylation and increases mitochondrial fission.
  
  
  CONCLUSIONS
  Our results suggest a novel molecular pathway for regulating mitochondrial dynamics and neuronal death. Nlrx1 may play an important role in neurodegenerative diseases, where necrosis is a prominent factor.
authors.affiliation: Program of Immunology, Department of Paediatrics, CR-CHUS, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, Canada. emilie.Imbeault@USherbrooke.ca.
authors.forename: Emilie
authors.initials: E
authors.lastname: Imbeault
authors.affiliation: Program of Immunology, Department of Paediatrics, CR-CHUS, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, Canada. t.mahvelati@gmail.com.
authors.forename: Tara M
authors.initials: TM
authors.lastname: Mahvelati
authors.affiliation: Institut fuer Zellbiologie, Universitaet Bayreuth, Bayreuth, Germany. ralf.braun@uni-bayreuth.de.
authors.forename: Ralf
authors.initials: R
authors.lastname: Braun
authors.affiliation: Montreal Neurological Institute, McGill University, Montréal, QC, Canada. pavel.gris@mcgill.ca.
authors.forename: Pavel
authors.initials: P
authors.lastname: Gris
authors.affiliation: Program of Immunology, Department of Paediatrics, CR-CHUS, Faculty of Medicine and Health Sciences, University of Sherbrooke, Sherbrooke, QC, Canada. denis.gris@usherbrooke.ca.
authors.forename: Denis
authors.initials: D
authors.lastname: Gris
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D034281:Dynamins / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D063154:Mitochondrial Dynamics / Q000187:drug effects; D024101:Mitochondrial Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D018696:Neuroprotective Agents / Q000494:pharmacology; D052999:Quinazolinones / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology
chemical_list: C549041:3-(2,4-dichloro-5-methoxyphenyl)-2-sulfanyl-4(3H)-quinazolinone; D024101:Mitochondrial Proteins; C555655:NLRX1 protein, mouse; D018696:Neuroprotective Agents; D052999:Quinazolinones; D012402:Rotenone; C486391:Dnm1l protein, mouse; D034281:Dynamins