title: Biodegradation of nicotine by a novel strain Pusillimonas.
abstract: A novel strain, Pusillimonas sp. T2, which is capable of degrading nicotine, was isolated and identified. This strain could completely degrade 500 mg/L nicotine within 8 h at 30 °C, pH 7.0. Six intermediates were detected and identified as 6-hydroxy-nicotine, 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine, 2,6-dihydroxypyridine, 6-hydroxy-3-succinoyl-pyridine and 2,5-dihydroxypyridine. Activities of 6-hydroxy-3-succinoyl-pyridine hydroxylase and 2,6-dihydroxypyridine hydroxylase were demonstrated in the cell extracts of strain T2, indicating that this strain may employ a novel variant of the pyridine and pyrrolidine pathways that is different from those of other species. This study provides the first evidence that Pusillimonas bacteria participate in nicotine degradation.
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: mayun@zjut.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: weroti@163.com.
authors.forename: Rongti
authors.initials: R
authors.lastname: Wen
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: qiujiguo@zjut.edu.cn.
authors.forename: Jiguo
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: 13757127801@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Hong
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: liumengpfr@163.com.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.affiliation: Research Center of Environmental Science, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. Electronic address: smzh327@163.com.
authors.forename: Dou
authors.initials: D
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D020562:Betaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002681:China; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009538:Nicotine / Q000031:analogs & derivatives / Q000378:metabolism*; D010802:Phylogeny; D011725:Pyridines / Q000378:metabolism; D011759:Pyrrolidines / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D013386:Succinates / Q000378:metabolism
chemical_list: C507268:6-hydroxy-3-succinoyl-pyridine; C502317:6-hydroxynicotine; D011725:Pyridines; D011759:Pyrrolidines; D012722:Sewage; D013386:Succinates; C089431:2,6-dihydroxypyridine; D009538:Nicotine; D006899:Mixed Function Oxygenases; C023666:pyridine; C065452:2,5-dihydroxypyridine
keywords: Degradation pathway; Intermediates; Nicotine; Pusillimonas sp. T2