title: In vitro transdifferentiation of human cultured CD34+ stem cells into oligodendrocyte precursors using thyroid hormones.
abstract: The extent of myelination on the axon promotes transmission of impulses in the neural network, any disturbances in this process results in the neurodegenerative condition. Transplantation of oligodendrocyte precursors that supports in the regeneration of axons through myelination is an important step in the restoration of damaged neurons. Therefore, in the present study, the differentiation of human CD34+ stem cells into oligodendrocytes was carried out. The pure human CD34+ culture developed from the stem cells obtained from a peripheral blood of a donor were subjected to oligodendrocyte differentiation medium (ODM). The ODM at a concentration of 40ng/ml thyroxine, 40ng/ml 3,3',5-tri-iodo-thyronine showed distinct morphological changes from day 6 to 9 with cells exhibiting conspicuous stellate morphology and extensive foot processes. The real-time PCR analysis showed prominent expression of Olig2, CNPase, PDGFRα and PLP1/DM20 in the differentiated cells confirming the formed cells are oligodendrocyte precursors. The expression of these genes increased from days 6 to 9 corresponding to the morphological changes observed with almost no expression of GFAP+ cells. The distinct CNPase activity was observed in these differentiated cells compared to normal CD34+ stem cells correlating with results of real-time PCR conclusively explains the development of oligodendrocytes from human CD34+ stem cells.
authors.affiliation: Stem cell Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
authors.forename: Katari
authors.initials: K
authors.lastname: Venkatesh
authors.affiliation: Stem cell Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
authors.forename: Lokanathan
authors.initials: L
authors.lastname: Srikanth
authors.affiliation: Department of Neurology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
authors.forename: Bhuma
authors.initials: B
authors.lastname: Vengamma
authors.affiliation: Department of Hematology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
authors.forename: Chodimella
authors.initials: C
authors.lastname: Chandrasekhar
authors.affiliation: Department of Neurosurgery, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India.
authors.forename: Bodapati Chandra Mouleshwara
authors.initials: BC
authors.lastname: Prasad
authors.affiliation: Stem cell Laboratory, Department of Biotechnology, Sri Venkateswara Institute of Medical Sciences, Tirupati, Andhra Pradesh, 517507, India. Electronic address: sarmasvims@gmail.com.
authors.forename: Potukuchi Venkata Gurunadha Krishna
authors.initials: PV
authors.lastname: Sarma
mesh_terms: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D054338:Cell Transdifferentiation; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008297:Male; D018991:Myelin Proteolipid Protein / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D013974:Thyroxine / Q000494:pharmacology*; D014285:Triiodothyronine, Reverse / Q000494:pharmacology*
chemical_list: D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D018952:Antigens, CD34; D051792:Basic Helix-Loop-Helix Proteins; D003470:Culture Media; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha; D013974:Thyroxine; D014285:Triiodothyronine, Reverse; C405708:OLIG2 protein, human; C472930:PLP1 protein, human
keywords: CD34+ stem cells; Oligodendrocyte differentiation medium; Oligodendrocytes; Thyroid hormones