title: [Effect of miR-92b on migration, adhesion and invasion of human gastric cancer cell line SGC7901].
abstract: OBJECTIVE
  To investigate the effect of miR-92b on the migration, adhesion and invasion of gastric cancer cell line SGC7901.
  
  
  METHODS
  The miR-92b inhibitor and mimics were transiently transfected in SGC7901 cells. The changes in the migration, adhesion and invasion of the transfected cells were tested with wound healing assay, Transwell migration assay, matrigel adhesion and Transwell invasion assay. The cellular expression of E-cadherin, vimentin, Akt and p-Akt were analyzed by Western blotting.
  
  
  RESULTS
  The migration, adhesion and invasion assays showed that transfection with the inhibitor of miR-92b obviously decreased the numbers of gastric cancer cells. The expression of E-cadherin, AKT, and pAKT increased and vimentin decreased significantly in the cells transfected with the inhibitor of miR-92b. Transfection with the mimics of miR-92b produced opposite effects in SGC7901 cells.
  
  
  CONCLUSIONS
  miR-92b promotes the migration, adhesion and invasion of human gastric cancer cell line SGC7901 by mediating epithelial-mesenchymal transition, and may accelerate tumor cell metastasis via signaling pathways other than PI3K/Akt pathway.
authors.affiliation: Department of Geriatric Gastroenterology, General Hospital of PLA, Beijing 100853, China.E-mail: weihua_wang1981@hotmail.com.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wang
authors.forename: Changzheng
authors.initials: C
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Benyan
authors.initials: B
authors.lastname: Wu
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000473:pathology*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D014746:Vimentin; D051057:Proto-Oncogene Proteins c-akt