title: Effects of dietary probiotic supplementation on LXRα and CYP7α1 gene expression, liver enzyme activities and fat metabolism in ducks.
abstract: 1. The objective of this study was to investigate the effects of dietary probiotic supplementation on liver X receptor alpha (LXRα) and cholesterol 7α-hydroxylase (CYP7α1) mRNA levels, protein enzymatic activities and fat metabolism in Cherry Valley Pekin ducks. 2. A total of 750 one-day-old Cherry Valley Pekin ducks were randomly divided into 5 groups with three replicates of 50 ducks each in a completely randomised experiment. Each group was fed on a basal diet supplemented with 0, 500, 1000, 1500 or 2000 mg probiotics/kg. 3. Body rate and feed conversion ratio were highest and abdominal subcutaneous fat % was lowest at 1000 mg probiotic/kg. 4. The mRNA levels of LXRα and CYP7α1 in liver tissue was estimated by RT-PCR; serum triglyceride (TG) and total cholesterol (TC) concentrations were measured by ELISA. 5. The expression levels and enzyme activity of LXRα and CYP7α1 increased in conjunction with decreases in TG and TC concentrations following probiotic supplementation to a maximum at 1000 mg probiotics/kg and decreased thereafter. 6. It is concluded that dietary probiotics can enhance LXRα and CYP7α1 enzyme activities in the liver and reduce lipid concentrations and fat deposition in ducks.
authors.affiliation: a Jiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design , Yangzhou University , Yangzhou 225009 , Jiangsu , China.
authors.forename: Z
authors.initials: Z
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Mu
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: Zhu
authors.forename: C
authors.initials: C
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Lan
authors.forename: Q
authors.initials: Q
authors.lastname: Xu
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.forename: G
authors.initials: G
authors.lastname: Chen
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D030161:Avian Proteins / Q000235:genetics* / Q000378:metabolism; D002790:Cholesterol 7-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism; D004032:Diet / Q000662:veterinary; D004372:Ducks / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D050356:Lipid Metabolism; D008099:Liver / Q000201:enzymology; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors / Q000235:genetics* / Q000378:metabolism; D019936:Probiotics
chemical_list: D030161:Avian Proteins; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D002790:Cholesterol 7-alpha-Hydroxylase