title: [Calbindin-containing neurons of the ventral horn of murine spinal cord gray matter].
abstract: The study was performed in 4 C57black/6 mice to examine the neurons located in T(II), L(IV), L(V) and L(VI) segments of the spinal cord (SC) ventral horn, containing 28 kD calbindin (CAB) and 200 kD neurofilament (NF) proteins. To demonstrate immunoreactive neurons, the cells were labeled with antibodies against CAB and double labeled with antibodies against CAB and NF. The total cell population was demonstrated using NeuroTrace Red Fluorescent Nissl Stain. Results have shown that CAB-immunopositive neurons were identified in ventromedial area of the ventral horn at all SC levels and were represented by Renshaw cells. CAB-positive interneurons located in the medial area of the ventral horn were present only in SC lumbar segments. CAB-positive motorneurons that were identified in the medial area of the ventral horn, were present in one SC segment (L(IV)) and were also found to contain a NF protein.
authors.forename: V V
authors.initials: VV
authors.lastname: Porseva
authors.forename: V V
authors.initials: VV
authors.lastname: Shilkin
authors.forename: A A
authors.initials: AA
authors.lastname: Strelkov
authors.forename: P M
authors.initials: PM
authors.lastname: Masliukov
mesh_terms: D000818:Animals; D000870:Anterior Horn Cells / Q000166:cytology* / Q000378:metabolism*; D064026:Calbindins / Q000378:metabolism*; D008161:Lumbosacral Region; D051379:Mice
chemical_list: D064026:Calbindins