title: [XAF1 inhibits cell proliferation and induces apoptosis in human lung adenocarcinoma cell line A549 in vitro].
abstract: OBJECTIVE
  XAF1 is a factor necessary to inhibit tumor cell growth. Low XAF1 expression is associated with various tumor cells. The aim of this study is to investigate the effect and the mechanism of adenovirus vector Ad5/F35 mediated X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) on the inhibition of cell proliferation and the induction of apoptosis of human lung adenocarcinoma cell A549.
  
  
  METHODS
  Recombinant virus Ad5/F35-XAF1 and controlled virus Ad5/F35-NULL exhibited different multiplicities of infection (MOI) at the same time. mRNA and protein expressions of XAF1 were determined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot, respectively. Cell proliferation was observed by methyl thiazolyl tetrazolium (MTT) assay, and cell apoptosis was analyzed by FACS with Annexin V-FITC/PI double staining. The expressions of apoptosis-associated proteins, such as PARP, Caspase-3, and Caspase-8, were also determined by Western blot.
  
  
  RESULTS
  mRNA and protein expressions of XAF1 were significantly increased in human lung adenocarcinoma cell A549 after this cell was transfected with Ad5/F35-XAF1 for 48 h; these expressions were higher than those of the controlled group Ad5/F35-NULL. Cell proliferation was inhibited and apoptosis was induced in a dose-dependent manner in the Ad5/F35-XAF1 group. After Ad5/F35-XAF1 transfection was performed, the cleavage of apoptosis-associated proteins, such as PARP, Caspase-3, and Caspase-8, was activated.
  
  
  CONCLUSIONS
  Restored XAF1 expression inhibits cell proliferation and induces cell apoptosis in human lung adenocarcinoma cell line A549. Furthermore, XAF1 may activate associated apoptotic signaling pathways in A549 cell line.
authors.affiliation: The Dushu Lake Campus Class 3 Grade, 2012 Clinical Medicine, Soochow University, Suzhou 215123, China.
authors.forename: Donglai
authors.initials: D
authors.lastname: Chen
authors.affiliation: The Second Hospital Affiliated to Suzhou University, Suzhou 215123, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Zhang
authors.affiliation: The Second Hospital Affiliated to Suzhou University, Suzhou 215123, China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Sang
authors.affiliation: The Second Hospital Affiliated to Suzhou University, Suzhou 215123, China.
authors.forename: Rongying
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Cancer Molecular Genetics Laboratory of Soochow University, Suzhou 215123, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: The Second Hospital Affiliated to Suzhou University, Suzhou 215123, China.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D000077192:Adenocarcinoma of Lung; D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009363:Neoplasm Proteins; C417551:XAF1 protein, human