title: Study of the interactivity between mercury and cellular system labeled with carboxymethyl chitosan-coated quantum dots and its application in a real-time in-situ detection of mercury.
abstract: In this study, canine kidney cells (MDCK) are fluorescently labeled by carboxymethyl chitosan-coated CdTe quantum dots to obtain a stable fluorescence. Fluorescently labeled MDCK cells are incubated with Hg(2+) and passed flow cytometer to measure the mean fluorescence intensity, which shows [Hg(2+)] has a prominent quenching ability on the cells' fluorescence. The dose-dependent relation can be described by Stern-Volmer equation at the concentration range of 5-70 μg/L [Hg(2+)]. This method can be employed to determine the concentration of Hg(2+) in living cells by measuring the changes in fluorescence of the cellular system. The results show a relative standard deviation of 7.16% (n=11) and a recovery rate ranging from 92% to 103%, indicating a promising prospect of application on real-time in-situ analysis of [Hg(2+)] and its cytotoxic effects.
authors.affiliation: Wuhan Centers for Disease Prevention and Control, Wuhan 430022, Hubei Province, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: He
authors.affiliation: College of Resource and Environmental Science, Wuhan University, Wuhan 430079, Hubei Province, PR China. Electronic address: hosan9174@gmail.com.
authors.forename: Peijiang
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Wuhan Centers for Disease Prevention and Control, Wuhan 430022, Hubei Province, PR China.
authors.forename: Honghao
authors.initials: H
authors.lastname: Zhu
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry; D003199:Computer Systems; D004285:Dogs; D061985:Madin Darby Canine Kidney Cells; D008628:Mercury / Q000032:analysis*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013691:Tellurium / Q000737:chemistry
chemical_list: D019187:Cadmium Compounds; C514968:carboxymethyl-chitosan; D048271:Chitosan; D008628:Mercury; D013691:Tellurium; C028337:cadmium telluride
keywords: Carboxymethyl chitosan; Cell; Mercury; Quantum dots