title: Lipidomic approach towards deciphering anandamide effects in rat decidual cell.
abstract: Altered phospholipid (PL) metabolism has been associated with pregnancy disorders. Moreover, lipid molecules such as endocannabinoids (eCBs) and prostaglandins (PGs) are important mediators of reproductive events. In humans, abnormal decidualization has been linked with unexplained infertility, miscarriage and endometrial pathologies. Anandamide (AEA), the major eCB, induces apoptosis in rat decidual cells. In this study, the PL profile of rat decidual cells was characterized by a Mass spectrometry (MS) based lipidomic approach. Furthermore, we analyzed a possible correlation between changes in PL of rat decidual cells' membrane and AEA-induced apoptosis. We found an increase in phosphatidylserine and a reduction of cardiolipin and phophatidylinositol relative contents. In addition, we observed an increase in the content of alkyl(alkenyl) acylPL, plasmalogens, and of long chain fatty acids especially with high degrees of unsaturation, as well as an increase in lipid hydroperoxides in treated cells. These findings provide novel insights on deregulation of lipid metabolism by anandamide, which may display further implications in decidualization process.
authors.affiliation: UCIBIO, REQUIMTE, Laboratory of Biochemistry, Biological Sciences Department, Faculty of Pharmacy, University of Porto, Porto, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Almada
authors.forename: M Rosário
authors.initials: MR
authors.lastname: Domingues
authors.forename: M Luísa
authors.initials: ML
authors.lastname: Dória
authors.forename: Bruno M
authors.initials: BM
authors.lastname: Fonseca
authors.forename: Natércia A
authors.initials: NA
authors.lastname: Teixeira
authors.forename: Georgina
authors.initials: G
authors.lastname: Correia-da-Silva
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D063386:Cannabinoid Receptor Agonists / Q000494:pharmacology*; D003656:Decidua / Q000166:cytology* / Q000187:drug effects*; D063388:Endocannabinoids / Q000494:pharmacology*; D005260:Female; D008054:Lipid Peroxides / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D010955:Plasmalogens; D053284:Polyunsaturated Alkamides / Q000494:pharmacology*; D011247:Pregnancy; D051381:Rats
chemical_list: D001095:Arachidonic Acids; D063386:Cannabinoid Receptor Agonists; D063388:Endocannabinoids; D008054:Lipid Peroxides; D010743:Phospholipids; D010955:Plasmalogens; D053284:Polyunsaturated Alkamides; C078814:anandamide