title: Long-term in vitro cultivation of Borrelia miyamotoi.
abstract: Borrelia are fastidious bacteria some of which are difficult to grow in vitro. Here, we report a method for successful continuous in vitro cultivation of the emerging pathogen Borrelia miyamotoi. The type and quantity of serum as well as the atmosphere were critical for successful in vitro cultivation. Optimal growth was achieved using 50% pooled human serum and an atmosphere of 6% CO2.
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany. Electronic address: gabriele.margos@lgl.bayern.de.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Margos
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Stockmeier
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Hizo-Teufel
authors.affiliation: German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Hepner
authors.affiliation: Yale School of Public Health, New Haven, CT, USA.
authors.forename: Durland
authors.initials: D
authors.lastname: Fish
authors.affiliation: IS Insect Services GmbH, Berlin, Germany.
authors.forename: Hans
authors.initials: H
authors.lastname: Dautel
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Sing
authors.affiliation: German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Eldina
authors.initials: E
authors.lastname: Dzaferovic
authors.affiliation: German National Reference Centre for Borrelia, Oberschleissheim, Germany; Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Melissa
authors.initials: M
authors.lastname: Rieger
authors.affiliation: German National Reference Centre for Borrelia, Oberschleissheim, Germany; Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Jungnick
authors.affiliation: German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Binder
authors.affiliation: Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Reinhard K
authors.initials: RK
authors.lastname: Straubinger
authors.affiliation: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; German National Reference Centre for Borrelia, Oberschleissheim, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Fingerle
mesh_terms: D000818:Animals; D001272:Atmosphere; D001898:Borrelia / Q000235:genetics / Q000254:growth & development*; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D007814:Larva; D016513:Mice, SCID; D011817:Rabbits; D012061:Relapsing Fever / Q000382:microbiology*; D044967:Serum; D013997:Time Factors
chemical_list: D003470:Culture Media; D002245:Carbon Dioxide
keywords: Borrelia miyamotoi; In vitro cultivation; Relapsing fever spirochetes