title: [Expression and correlation of CIP2A and OPN in bladder cancer].
abstract: OBJECTIVE
  To explore the expressions of cancerous inhibitor of protein phosphatase 2A (CIP2A) and osteopontin (OPN) and evaluate their roles in bladder cancer.
  
  
  METHODS
  RNA was isolated from 38 cases of patients with bladder cancer and 12 cases of normal bladder tissue by TRIzol method from May 2010 to December 2012. And reverse transcription (RT)-PCR was used to detect the expressions of CIP2A and OPN. The expression levels of CIP2A and OPN in 99 cases of patients with bladder cancer and 12 cases of normal tissue were detected by immunohistochemical staining.
  
  
  RESULTS
  The positive expression rate of CIP2A mRNA and OPN mRNA were 76.32% (29/38) and 92.11% (35/38) in bladder cancer while there was no expression in normal tissue (both P < 0.05). The positive rates of CIP2A and OPN protein were 63.64% (63/99)and 84.85% (84/99)in cases of bladder cancer tissues while CIP2A was not detected in normal tissues. The positive expression rate of OPN in normal tissues was 2/12 (both P < 0.05). The CIP2A and OPN proteins were both expressed in 58/99 cases of bladder cancer tissues while neither of them was expressed in 13 cases. In 8 cases, CIP2A was expressed while OPN was not. In another 20 cases, OPN was expressed while CIP2A was not (r = 0.300, P < 0.05).
  
  
  CONCLUSIONS
  The expression levels of CIP2A and OPN in tissue of bladder cancer are higher than those of normal controls. And CIP2A and OPN may be used as indicators of biological behaviors and serve as new molecular diagnostic markers for bladder cancer.
authors.affiliation: Department of Urology, Second Hospital of Shandong University, Shandong University Urologic Institute, Jinan 250033, China.
authors.forename: Tianjia
authors.initials: T
authors.lastname: Ma
authors.forename: Pan
authors.initials: P
authors.lastname: Xiao
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yutang
authors.initials: Y
authors.lastname: Li
authors.forename: Peikun
authors.initials: P
authors.lastname: Shi
authors.affiliation: Department of Pathogen Biology, Shandong University, Jinan 250012, China. Email: zyb@sdu.edu.cn.
authors.forename: Yabin
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D001324:Autoantigens; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D053495:Osteopontin; D012333:RNA, Messenger; D001749:Urinary Bladder Neoplasms
chemical_list: D001324:Autoantigens; C500266:CIP2A protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D053495:Osteopontin