title: Photosynthetic production of glycerol by a recombinant cyanobacterium.
abstract: Cyanobacteria are prokaryotic organisms capable of oxygenic photosynthesis. Glycerol is an important commodity chemical. Introduction of phosphoglycerol phosphatase 2 from Saccharomyces cerevisiae into the model cyanobacterium Synechocystis sp. PCC6803 resulted in a mutant strain that produced a considerable amount of glycerol from light, water and COPhotosynthetic production . Mild salt stress (200 mM NaCl) on the cells led to an increase of the extracellular glycerol concentration of more than 20%. Under these conditions the mutant accumulated glycerol to an extracellular concentration of 14.3 mM after 17 days of culturing.
authors.affiliation: Molecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Philipp
authors.initials: P
authors.lastname: Savakis
authors.affiliation: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Tan
authors.affiliation: Molecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.affiliation: Molecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Filipe
authors.initials: F
authors.lastname: Branco dos Santos
authors.affiliation: Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Lu
authors.affiliation: Molecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: K.J.Hellingwerf@uva.nl.
authors.forename: Klaas J
authors.initials: KJ
authors.lastname: Hellingwerf
mesh_terms: D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D004274:DNA, Recombinant / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000032:analysis / Q000378:metabolism*; D060847:Metabolic Engineering; D009154:Mutation; D009997:Osmotic Pressure; D010788:Photosynthesis / Q000502:physiology*; D012965:Sodium Chloride
chemical_list: D004274:DNA, Recombinant; D012965:Sodium Chloride; D005990:Glycerol
keywords: Cell factory; Glycerol; Metabolic engineering; Synechocystis