title: [Winter viral ecology in a pediatric intensive care unit: a prospective study].
abstract: BACKGROUND
  Viral respiratory infections are common in children, most of which are due to a virus. They can lead to serious infections, and these children may require treatment in a pediatric intensive care unit (PICU). This prospective study evaluated the epidemiology of respiratory viruses and associated illnesses among children hospitalized in a PICU during the three winter months of 2012-2013.
  
  
  METHODS
  All the children admitted to the PICU, University Hospital of Caen, France, were included. Nasal swabs were collected and specimens were tested using a single real-time PCR (polymerase chain reaction).
  
  
  RESULTS
  Of the 105 patients admitted to the PICU during the study period, 84 fulfilled the inclusion criteria. The "respiratory group" included 37 patients with respiratory symptoms at admission while the "nonrespiratory group" included 47 patients with no respiratory symptoms. The 84 nasal swabs collected included 54 that were considered positive (64.3%) and 70 viruses were detected. The most commonly detected virus was RSV (n=28; 40.0% positive samples), followed by HRV (n=24; 34.3%). Viruses were more frequently detected in the respiratory (86.5%) than in the nonrespiratory (42.6%) group (P<0.001). Statistical analysis by subgroups revealed that RSV infections were significantly more frequent in the respiratory group (54.1%) than in the nonrespiratory group (6.4%) (P<0.001). There was no difference for HRV (32.4% and 27.7%) or for the other viruses. No difference in duration of hospitalization or duration of mechanical ventilation was demonstrated depending on the virus detected.
  
  
  CONCLUSIONS
  The use of the very sensitive multiplex PCR technique increased virus detection rates in both symptomatic and asymptomatic subjects.
  
  
  CONCLUSIONS
  We have confirmed the frequency of RSV infections in a PICU and found that many patients without respiratory symptoms have respiratory infections caused by viruses. The impact of these infections on patient outcome should now be analyzed in order to demonstrate the role played by respiratory viruses.
authors.affiliation: Unité de réanimation pédiatrique, CHU de Caen, avenue Côte-de-Nacre, 14033 Caen, France. Electronic address: guillaume.mortamet@nck.aphp.fr.
authors.forename: G
authors.initials: G
authors.lastname: Mortamet
authors.affiliation: Département de biostatistiques et de recherche clinique, CHU de Caen, avenue Georges-Clémenceau, 14033 Caen, France.
authors.forename: R
authors.initials: R
authors.lastname: Morello
authors.affiliation: Unité de réanimation pédiatrique, CHU de Caen, avenue Côte-de-Nacre, 14033 Caen, France.
authors.forename: M
authors.initials: M
authors.lastname: Jokic
authors.affiliation: Laboratoire de virologie, CHU de Caen, avenue Georges-Clémenceau, 14033 Caen, France; Université de Caen, EA4655, France.
authors.forename: A
authors.initials: A
authors.lastname: Vabret
authors.affiliation: Laboratoire de virologie, CHU de Caen, avenue Georges-Clémenceau, 14033 Caen, France.
authors.forename: M
authors.initials: M
authors.lastname: Leroux
authors.affiliation: Service de pédiatrie, CHU de Caen, avenue Côte-de-Nacre, 14033 Caen, France; Université de Caen, EA4655, France.
authors.forename: J
authors.initials: J
authors.lastname: Brouard
authors.affiliation: Laboratoire de virologie, CHU de Caen, avenue Georges-Clémenceau, 14033 Caen, France; Université de Caen, EA4655, France.
authors.forename: J
authors.initials: J
authors.lastname: Dina
mesh_terms: D005260:Female; D006760:Hospitalization; D006801:Humans; D007223:Infant; D015278:Intensive Care Units, Pediatric; D008297:Male; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D012621:Seasons