title: Clinical application of ultrasound for preparation of (99m)Tc-sestamibi complex.
abstract: OBJECTIVE
  The main aim of this investigation is the clinical application of ultrasound irradiation technique as an alternative method to reconstitute sestamibi kits in comparison of water boiling bath method.
  
  
  METHODS
  The 740-3700 MBq (20-100 mCi) (99m)Tc-MIBI (sestamibi) complex samples were prepared due to ultrasound irradiation technique or boiled water bath method as a standard method. Twenty patients (8 men and 12 women; age range 30-72, median 52.45 years) have been referred to Golestan hospital for myocardial perfusion imaging (MPI). The subjects have been divided randomly into group A (3 men, 7 women, age range 36-67, median 51.7 years) and group B (5 men, 5 women, age range 30-72, median 50.3 years), respectively. The (99m)Tc-MIBI radiopharmaceuticals have been prepared by Ultrasound irradiation technique administrated to group A and (99m)Tc-MIBI complex samples due to the boiled water bath technique administrated to the other group. For all patients, the 2-day stress/rest MPI protocol was performed.
  
  
  RESULTS
  The radio-HPLC and TLC studies have indicated that the (99m)Tc-MIBI complex samples with good yields could be prepared successfully due to new developed technique. The scintigraphy imaging studies have demonstrated that the (99m)Tc-sestamibi prepared due to the above-mentioned modalities shows very identical biodistribution in the heart, thyroid, lung, liver, gallbladder, kidneys, stomach, large intestine and bladder of the subjects. Any unexpected accumulation of radiotracer samples have not been observed in our approach.
  
  
  CONCLUSIONS
  The ultrasound irradiation technique is convenient and sufficient method to prepare (99m)Tc-sestamibi. It can be recommended as an alternative method to reconstitute sestamibi kits particularly in emergency situations to reduce potentially medical risk by avoiding any delay in acute therapy for myocardial infarction.
authors.affiliation: School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, Doroudi-a@ajums.ac.ir.
authors.forename: Alireza
authors.initials: A
authors.lastname: Doroudi
authors.forename: Mostafa
authors.initials: M
authors.lastname: Erfani
authors.forename: Behzad
authors.initials: B
authors.lastname: Norouzi
authors.forename: Seyyed Mostafa
authors.initials: SM
authors.lastname: Saadati
authors.forename: Ali
authors.initials: A
authors.lastname: Kiasat
authors.forename: Faramarz
authors.initials: F
authors.lastname: Ahmadi
authors.forename: Behrooz
authors.initials: B
authors.lastname: Etesami
authors.forename: Mohammad Hadi
authors.initials: MH
authors.lastname: Baghersad
mesh_terms: D000328:Adult; D000368:Aged; D002851:Chromatography, High Pressure Liquid; D004339:Drug Compounding / Q000379:methods*; D005260:Female; D005704:Gallbladder / Q000000981:diagnostic imaging; D006321:Heart / Q000000981:diagnostic imaging; D006358:Hot Temperature; D006801:Humans; D008099:Liver / Q000000981:diagnostic imaging; D008168:Lung / Q000000981:diagnostic imaging; D008297:Male; D008875:Middle Aged; D055414:Myocardial Perfusion Imaging / Q000379:methods; D019275:Radiopharmaceuticals; D011897:Random Allocation; D017256:Technetium Tc 99m Sestamibi; D013961:Thyroid Gland / Q000000981:diagnostic imaging; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D000069453:Ultrasonic Waves; D014867:Water; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D019275:Radiopharmaceuticals; D014867:Water; D017256:Technetium Tc 99m Sestamibi