title: Improving xylitol production through recombinant expression of xylose reductase in the white-rot fungus Phanerochaete sordida YK-624.
abstract: We generated an expression construct consisting of the xylose reductase (XR) gene (xr) from Phanerochaete chrysosporium. Transformant X7 exhibited increased xylitol production and markedly higher XR activities than the wild-type strain. RT-PCR analysis demonstrated that the increased XR activity was associated with constant expression of the recombinant xr gene.
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Hirabayashi
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Jianqiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
authors.affiliation: Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: ahhirai@ipc.shizuoka.ac.jp.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014993:Xylitol / Q000096:biosynthesis*; D014994:Xylose / Q000378:metabolism*
chemical_list: D014994:Xylose; D000449:Aldehyde Reductase; D014993:Xylitol
keywords: Phanerochaete sordida YK-624; Recombinant expression; White-rot fungi; Xylitol; Xylose reductase