title: Nanostructured lipid carriers as novel drug delivery system for lung cancer gene therapy.
abstract: BACKGROUND
  Nanostructured lipid carriers (NLC) are potentially good colloidal drug carriers for gene delivery. They are advised to be the second lifetime of lipid nanocarriers.
  
  
  OBJECTIVE
  The aim of this study is to develop novel modified NLC as nanomedicine for delivery of plasmid-containing enhanced green fluorescence protein (pEGFP). This system could target the lung cancer cells through receptor-mediated pathways to increase the nuclear uptake of genetic materials.
  
  
  METHODS
  In the present study, pEGFP-loaded NLC (NLC/pEGFP) were prepared. Transferrin (Tf) containing ligands were used for the surface coating of the vectors. In vitro transfection efficiency of the modified vectors was evaluated in human alveolar adenocarcinoma cell line (A549 cells) and in vivo transfection efficiency of the modified vectors was evaluated on mice bearing A549 cells model.
  
  
  RESULTS
  Tf-modified NLC/pEGFP (Tf-NLC/pEGFP) has a particle size of 157 nm, and ∼ 82% of gene loading quantity. Tf-NLC/pEGFP displayed remarkably higher transfection efficiency than non-modified NLC/pEGFP both in vitro and in vivo.
  
  
  CONCLUSIONS
  The results demonstrate that the novel NLC gene delivery system offers an effective strategy for lung cancer gene therapy.
authors.affiliation: a Department of Respiratory Medicine , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China .
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Han
authors.affiliation: b Center of Interventional Therapy, Ji'nan Infectious Disease Hospital , Ji'nan , People's Republic of China , and.
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.affiliation: a Department of Respiratory Medicine , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China .
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: a Department of Respiratory Medicine , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China .
authors.forename: Jiping
authors.initials: J
authors.lastname: Sun
authors.affiliation: a Department of Respiratory Medicine , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China .
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Li
authors.affiliation: a Department of Respiratory Medicine , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China .
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: c Department of Hematology , General Hospital of Ji'nan Command , Ji'nan , People's Republic of China.
authors.forename: Fansheng
authors.initials: F
authors.lastname: Kong
mesh_terms: D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000188:drug therapy; D000818:Animals; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D049452:Green Fluorescent Proteins / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy*; D051379:Mice; D050997:Nanomedicine / Q000379:methods; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000008:administration & dosage / Q000737:chemistry; D014162:Transfection / Q000379:methods
chemical_list: D004337:Drug Carriers; D008055:Lipids; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Active targeting; gene therapy; nanostructured lipid carriers; transferrin modification