title: Differential regulation of microtubule severing by APC underlies distinct patterns of projection neuron and interneuron migration.
abstract: Coordinated migration of distinct classes of neurons to appropriate positions leads to the formation of functional neuronal circuitry in the cerebral cortex. The two major classes of cortical neurons, interneurons and projection neurons, utilize distinctly different modes (radial versus tangential) and routes of migration to arrive at their final positions in the cerebral cortex. Here, we show that adenomatous polyposis coli (APC) modulates microtubule (MT) severing in interneurons to facilitate tangential mode of interneuron migration, but not the glial-guided, radial migration of projection neurons. APC regulates the stability and activity of the MT-severing protein p60-katanin in interneurons to promote the rapid remodeling of neuronal processes necessary for interneuron migration. These findings reveal how severing and restructuring of MTs facilitate distinct modes of neuronal migration necessary for laminar organization of neurons in the developing cerebral cortex.
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Tae-Yeon
authors.initials: TY
authors.lastname: Eom
authors.affiliation: Department of Psychiatry, Neuroscience Program, and Nina Ireland Laboratory of Developmental Neurobiology, University of California, San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: Amelia
authors.initials: A
authors.lastname: Stanco
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Jiami
authors.initials: J
authors.lastname: Guo
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Wilkins
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Danielle
authors.initials: D
authors.lastname: Deslauriers
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Yan
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Chase
authors.initials: C
authors.lastname: Monckton
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Blair
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Eesim
authors.initials: E
authors.lastname: Oon
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Abby
authors.initials: A
authors.lastname: Perez
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Salas
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Oh
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Ghukasyan
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: William D
authors.initials: WD
authors.lastname: Snider
authors.affiliation: Department of Psychiatry, Neuroscience Program, and Nina Ireland Laboratory of Developmental Neurobiology, University of California, San Francisco, San Francisco, CA 94158-2324, USA.
authors.forename: John L R
authors.initials: JL
authors.lastname: Rubenstein
authors.affiliation: UNC Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA. Electronic address: anton@med.unc.edu.
authors.forename: E S
authors.initials: ES
authors.lastname: Anton
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000483:Alleles; D000818:Animals; D064189:Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism*; D002454:Cell Differentiation; D002465:Cell Movement; D002540:Cerebral Cortex / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D017353:Gene Deletion; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007395:Interneurons / Q000378:metabolism*; D000074201:Katanin; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D013997:Time Factors
chemical_list: C577336:Anapc1 protein, mouse; D064189:Apc1 Subunit, Anaphase-Promoting Complex-Cyclosome; D049452:Green Fluorescent Proteins; D000251:Adenosine Triphosphatases; D000074201:Katanin; C552938:Katna1 protein, mouse