title: One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase.
abstract: We describe a simple, efficient process for the production of 6-kestose, a trisaccharide with well-documented prebiotic properties. A key factor is the use of a yeast transformant expressing an engineered version of Saccharomyces invertase with enhanced transfructosylating activity. When the yeast transformant was grown with 30 % sucrose as the carbon source, 6-kestose accumulated up to ca. 100 g/L in the culture medium. The 6-kestose yield was significantly enhanced (up to 200 g/L) using a two-stage process carried out in the same flask. In the first stage, the culture was grown in 30 % sucrose at physiological temperature (30 °C) to allow overexpression of the invertase. In the second stage, sucrose was added to the culture at high concentration (60 %) and the temperature shifted to 50 °C. In both cases, 6-kestose was synthesized with high specificity, representing more than 95 % of total FOS.
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Av. Agustín Escardino 7, 46980, Paterna, Valencia, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Marín-Navarro
authors.forename: David
authors.initials: D
authors.lastname: Talens-Perales
authors.forename: Julio
authors.initials: J
authors.lastname: Polaina
mesh_terms: D003470:Culture Media; D007218:Industrial Microbiology; D009844:Oligosaccharides / Q000096:biosynthesis*; D015202:Protein Engineering; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D013395:Sucrose / Q000737:chemistry; D014170:Transformation, Genetic; D014312:Trisaccharides / Q000096:biosynthesis; D043324:beta-Fructofuranosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D003470:Culture Media; D009844:Oligosaccharides; D029701:Saccharomyces cerevisiae Proteins; D014312:Trisaccharides; C116580:fructooligosaccharide; C073432:6-kestose; D013395:Sucrose; C468437:SUC2 protein, S cerevisiae; D043324:beta-Fructofuranosidase