title: Microarray analysis of long non-coding RNAs in COPD lung tissue.
abstract: OBJECTIVE
  Long noncoding RNAs (lncRNAs) play an important role in the pathogenesis of many human diseases. In this study, we provide the description of genome-wide lncRNA expression in the lung tissue of non-smokers without Chronic obstructive pulmonary disease (COPD), of smokers without COPD and of smokers with COPD.
  
  
  METHODS
  RNA was extracted from human lung tissue and analysed using an Agilent Human lncRNA + mRNA Array v2.0 system.
  
  
  RESULTS
  39,253 distinct lncRNA transcripts were detected in the lung tissues of all subjects. In smokers without COPD 87 lncRNAs were significantly up-regulated and 244 down-regulated compared to non-smokers without COPD with RNA50010|UCSC-9199-1005 and RNA58351| CombinedLit_316_550, the most over- and under-regulated, respectively. In contrast, in COPD patients 120 lncRNAs were over-expressed and 43 under-expressed compared with smokers without COPD with RNA44121|UCSC-2000-3182 and RNA43510|UCSC-1260-3754 being the most over- and under-regulated, respectively. Gene Ontology (GO) and pathway analysis indicated that cigarette smoking was associated with activation of metabolic pathways, whereas COPD transcripts were associated with 'hematopoietic cell lineage', intermediary metabolism and immune system processes.
  
  
  CONCLUSIONS
  We conclude that the altered expression of lncRNAs might play partial role in pathways implicated in COPD onset and progression such as intermediary metabolism and the immune response.
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Bi
authors.forename: Ji
authors.initials: J
authors.lastname: Zhou
authors.forename: Dandan
authors.initials: D
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Lingling
authors.initials: L
authors.lastname: Li
authors.forename: Like
authors.initials: L
authors.lastname: Yu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Mao
authors.initials: M
authors.lastname: Huang
authors.forename: Ian M
authors.initials: IM
authors.lastname: Adcock
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Barnes
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D063990:Gene Ontology; D006801:Humans; D008168:Lung / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics* / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D012907:Smoking / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger