title: FAP206 is a microtubule-docking adapter for ciliary radial spoke 2 and dynein c.
abstract: Radial spokes are conserved macromolecular complexes that are essential for ciliary motility. A triplet of three radial spokes, RS1, RS2, and RS3, repeats every 96 nm along the doublet microtubules. Each spoke has a distinct base that docks to the doublet and is linked to different inner dynein arms. Little is known about the assembly and functions of individual radial spokes. A knockout of the conserved ciliary protein FAP206 in the ciliate Tetrahymena resulted in slow cell motility. Cryo-electron tomography showed that in the absence of FAP206, the 96-nm repeats lacked RS2 and dynein c. Occasionally, RS2 assembled but lacked both the front prong of its microtubule base and dynein c, whose tail is attached to the front prong. Overexpressed GFP-FAP206 decorated nonciliary microtubules in vivo. Thus FAP206 is likely part of the front prong and docks RS2 and dynein c to the microtubule.
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602.
authors.forename: Krishna Kumar
authors.initials: KK
authors.lastname: Vasudevan
authors.affiliation: Department of Biology, Rosenstiel Center, Brandeis University, Waltham, MA 02454.
authors.forename: Kangkang
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Cell Biology, Emory University, Atlanta, GA 30303.
authors.forename: Lea M
authors.initials: LM
authors.lastname: Alford
authors.affiliation: Department of Cell Biology, Emory University, Atlanta, GA 30303.
authors.forename: Winfield S
authors.initials: WS
authors.lastname: Sale
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH 03755.
authors.forename: Erin E
authors.initials: EE
authors.lastname: Dymek
authors.affiliation: Department of Biological Sciences, Dartmouth College, Hanover, NH 03755.
authors.forename: Elizabeth F
authors.initials: EF
authors.lastname: Smith
authors.affiliation: Department of Biological Sciences, State University of New York, Buffalo, NY 14260.
authors.forename: Todd
authors.initials: T
authors.lastname: Hennessey
authors.affiliation: Department of Cell Biology, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland Department of Animal Physiology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Joachimiak
authors.affiliation: Department of Cell Biology, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Urbanska
authors.affiliation: Department of Cell Biology, Nencki Institute of Experimental Biology, 02-093 Warsaw, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Wloga
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045.
authors.forename: William
authors.initials: W
authors.lastname: Dentler
authors.affiliation: Department of Biology, Rosenstiel Center, Brandeis University, Waltham, MA 02454.
authors.forename: Daniela
authors.initials: D
authors.lastname: Nicastro
authors.affiliation: Department of Cellular Biology, University of Georgia, Athens, GA 30602.
authors.forename: Jacek
authors.initials: J
authors.lastname: Gaertig
mesh_terms: D054468:Axoneme / Q000378:metabolism*; D002923:Cilia / Q000378:metabolism / Q000502:physiology; D004398:Dyneins / Q000378:metabolism*; D055032:Electron Microscope Tomography; D055786:Gene Knockout Techniques; D008870:Microtubules / Q000378:metabolism* / Q000648:ultrastructure; D008958:Models, Molecular; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D013768:Tetrahymena / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D015800:Protozoan Proteins; D004398:Dyneins