title: [Expression of MiRNA-21 in diffuse large B cell lymphoma and its significance].
abstract: MicroRNA-21 (miR-21) is considered to play a key role in many cellular processes, affecting tumorigenesis by inhibiting target gene expression. However, its role in diffuse large B-cell lymphoma (DLBCL) is still unclear, and there are no in depth studies on relationship between miR-21 and cellular phenotype. This study was aimed to investigated the expression and role of miR-21 in the regulation of cell biological behavior in DLBCL. The expressions of miR-21 in three DLBCL cell lines were detected by real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The possible roles of miR-21 in the biological and behavioral properties of DLBCL were explored by transfection of anti-miR-21 for miR-21 knockdown. In addition, PDCD4 and PTEN were assessed by luciferase reporter assay, qRT-PCR and Western blot. The results revealed that miR-21 expression was significantly upregulated in activated B-cell-like DLBCL cells as compared to germinal centre-like DLBCL cells. The inhibition of miR-21 could induce suppression of proliferation and invasion, as well as increase apoptosis in DLBCL. Moreover, knockdown of miR-21 increased the expressions of PDCD4 and PTEN at the protein level but not at the mRNA level. It is concluded the miR-21 can regulate proliferation, invasion, and apoptosis, so it has a potential therapeutic application in DLBCL.
authors.affiliation: Department of Internal Medicine, Southeast University Medical Shool, Nanjiang 210009, Jiangsu Province, China.
authors.forename: Guo-Qi
authors.initials: GQ
authors.lastname: Song
authors.affiliation: Department of Internal Medicine, Southeast University Medical Shool, Nanjiang 210009, Jiangsu Province, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gu
authors.affiliation: Department of Internal Medicine, Southeast University Medical Shool, Nanjiang 210009, Jiangsu Province, China.
authors.forename: Bang-Shun
authors.initials: BS
authors.lastname: He
authors.affiliation: Department of Internal Medicine, Southeast University Medical Shool, Nanjiang 210009, Jiangsu Province, China.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Pan
authors.affiliation: Department of Internal Medicine, Southeast University Medical Shool, Nanjiang 210009, Jiangsu Province, China. E-mail: shukuiwang1967@163.com.
authors.forename: Shu-Kui
authors.initials: SK
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D051059:PTEN Phosphohydrolase; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D015854:Up-Regulation
chemical_list: C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human