title: [Expression of gamma interferon during HPV and Chlamydia trachomatis infection in cervical samples].
abstract: BACKGROUND
  The aim of this study was to mesure the expression of gamma interferon in HPV and Chlamydia trachomatis infection in squamous intraepithelial lesions.
  
  
  METHODS
  Samples from 100 patients diagnosed by colposcopy with or without squamous intraepithelial lesions were used in the present study. Each patient was found to be infected by HPV and C.trachomatis. Relative gamma interferon mRNA expression was assessed using a real-time reverse transcriptase PCR assay (RT-PCR).
  
  
  RESULTS
  The relative units of expression of gamma interferon mRNA were 13, 1.8 and 0.3, for HPV and C.trachomatis co-infection, or HPV or C.trachomatis infection, respectively.
  
  
  CONCLUSIONS
  HPV and C.trachomatis could overstimulate the expression of gamma interferon.
authors.affiliation: Laboratorio de Biología Molecular, Centro de Investigación en Ciencias Médicas (CICMED), Posgrado en Ciencias de la Salud, Facultad de Medicina, Universidad Autónoma del Estado de México, Toluca, México.
authors.forename: María Del Carmen
authors.initials: Mdel C
authors.lastname: Colín-Ferreyra
authors.affiliation: Laboratorio de Biología Molecular, Centro de Investigación en Ciencias Médicas (CICMED), Universidad Autónoma del Estado de México; Asociación Científica Latina (ASCILA) y Ciprés Grupo Médico (CGM), Toluca, México.
authors.forename: Hugo
authors.initials: H
authors.lastname: Mendieta-Zerón
authors.affiliation: Coordinación del Investigación, Instituto Mexicano del Seguro Social (IMSS), Toluca, México.
authors.forename: María Del Socorro
authors.initials: Mdel S
authors.lastname: Romero-Figueroa
authors.affiliation: Departamento de Colposcopia, Hospital Materno-Perinatal «Mónica Pretelini», Instituto de Salud del Estado de México (ISEN), Toluca, México.
authors.forename: Migdania
authors.initials: M
authors.lastname: Martínez-Madrigal
authors.affiliation: Departamento de Colposcopia, Centro de Investigación en Ciencias Médicas (CICMED), Universidad Autónoma del Estado de México, Toluca, México.
authors.forename: Sergio
authors.initials: S
authors.lastname: Martínez-Pérez
authors.affiliation: Laboratorio de Biología Molecular, Centro de Investigación en Ciencias Médicas (CICMED), Universidad Autónoma del Estado de México, Toluca, México. Electronic address: cicmedarticulos@gmail.com.
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Domínguez-García
mesh_terms: D000328:Adult; D002690:Chlamydia Infections / Q000150:complications / Q000378:metabolism*; D002692:Chlamydia trachomatis; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis*; D030361:Papillomavirus Infections / Q000150:complications / Q000378:metabolism*; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000150:complications / Q000382:microbiology*
chemical_list: D007371:Interferon-gamma
keywords: Chlamydia trachomatis; Co-infection; Coinfección; Gamma interferon; Human papillomavirus; Interferón gamma; Virus del papiloma humano