title: Expression of Toll-like receptor 9 (TLR9) in the lungs and lymphoid tissue of pigs.
abstract: The pattern of distribution of Toll-like receptor 9 (TLR9) in different tissues varies between species. The aim of the present study was to describe the distribution of TLR9 expression in selected tissues and organs of healthy pigs at 3 weeks and 3 months of age. Representative formalin-fixed samples of lung, thymus and secondary lymphoid tissues were evaluated by immunohistochemistry. TLR9 positive staining was observed in epithelial cells, vascular endothelium and myoepithelial-like cells, as well as in cells of the alveolar septa of the lung. Antigen presenting cells of perifollicular zones (interdigitating, macrophage and dendritic-like cells) of the Peyer's patches, lymph nodes, spleen and thymus were also immunoreactive for TLR9. No differences were seen in TLR9 protein expression in tissues from the two age groups.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
authors.forename: Liudmila
authors.initials: L
authors.lastname: Kuzemtseva
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Pérez
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain; Department Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Spain.
authors.forename: Enric
authors.initials: E
authors.lastname: Mateu
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain; Department Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Spain.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Segalés
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA)- IRTA, Campus de la Universitat Autònoma de Barcelona (UAB), 08193 Bellaterra, Spain; Department Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Spain. Electronic address: laila.darwich@uab.cat.
authors.forename: Laila
authors.initials: L
authors.lastname: Darwich
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D007150:Immunohistochemistry / Q000662:veterinary; D008168:Lung / Q000378:metabolism; D008221:Lymphoid Tissue / Q000378:metabolism; D034421:Sus scrofa / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D051217:Toll-Like Receptor 9 / Q000235:genetics* / Q000378:metabolism
chemical_list: D051217:Toll-Like Receptor 9
keywords: Immunohistochemistry; Lung; Lymphoid tissue; Pig; Toll-like receptor 9