title: Polysaccharides of Dendrobium officinale induce aquaporin 5 translocation by activating M3 muscarinic receptors.
abstract: Dendrobium officinale is an herbal medicine that has been clinically used to promote body fluid production. Previous works demonstrated that D. officinale polysaccharides could ameliorate symptoms of salivary secretion of patients with Sjögren's syndrome and in a respective mice model. In the present study, we investigated the underlying mechanism by which D. officinale polysaccharides activate M3 muscarinic receptors and induce extracellular calcium influx, leading to the translocation of aquaporin 5, a water channel protein, to the apical membrane of human submandibular gland epithelial cells. Enzymatic treatment of D. officinale polysaccharides suggested that they are hydrolyzed but do not permeate cell membranes. This finding supports the pharmacological activity of D. officinale polysaccharides to promote salivary secretion.
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Liu
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Wingyan
authors.initials: W
authors.lastname: Chung
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Stephen Cho-Wing
authors.initials: SC
authors.lastname: Sze
authors.affiliation: Foshan University, Department of Pharmacy, Foshan, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Lixing
authors.initials: L
authors.lastname: Lao
authors.affiliation: School of Chinese Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000378:metabolism*; D031667:Dendrobium / Q000737:chemistry*; D004195:Disease Models, Animal; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D043587:Receptor, Muscarinic M3 / Q000187:drug effects / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000188:drug therapy*
chemical_list: D051402:Aquaporin 5; D010936:Plant Extracts; D011134:Polysaccharides; D043587:Receptor, Muscarinic M3