title: A novel procedure for purification of uridine 5'-monophosphate based on adsorption methodology using a hyper-cross-linked resin.
abstract: The conventional ion exchange process used for recovery of uridine 5'-monophosphate (UMP) from the enzymatic hydrolysate of RNA is environmentally harmful and cost intensive. In this work, an innovative benign process, which comprises adsorption technology and use of a hyper-cross-linked resin as a stationary phase is proposed. The adsorption properties of this kind of resin in terms of adsorption equilibrium as well as kinetics were evaluated. The influences of the operating conditions, i.e., initial UMP concentration, feed flow rate, and bed height on the breakthrough curves of UMP in the fixed bed system were investigated. Subsequently, a chromatographic column model was established and validated for the prediction of the experimentally attained breakthrough curves of UMP and the main impurity component (phosphate ion) with a real enzymatic hydrolysate of RNA as a feed mixture. At the end of this paper, the crystallization of UMP was carried out. The purity of the final product (uridine 5'-monophosphate disodium, UMPNa2) of over 99.5 % was obtained.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, Nanjing, 210009, People's Republic of China.
authors.forename: Jinglan
authors.initials: J
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Zhuang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Jiao
authors.forename: Xu
authors.initials: X
authors.lastname: Ke
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000327:Adsorption; D002845:Chromatography; D003432:Cross-Linking Reagents / Q000737:chemistry; D003460:Crystallization; D000431:Ethanol / Q000737:chemistry; D006868:Hydrolysis; D007700:Kinetics; D008962:Models, Theoretical; D012313:RNA / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D014542:Uridine Monophosphate / Q000737:chemistry* / Q000302:isolation & purification*; D014867:Water / Q000737:chemistry; D018508:Water Purification / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D012997:Solvents; D014867:Water; D000431:Ethanol; D012313:RNA; D014542:Uridine Monophosphate