title: Evaluation of Cytotoxicity of Dentin Desensitizing Products.
abstract: OBJECTIVE
  To evaluate the cytotoxic effects of the dentin desensitizing products (DDPs) used in the treatment of dentin hypersensitivity on cultured human gingival and pulpal fibroblast cells.
  
  
  METHODS
  The cytotoxic effects of DDPs (Smart Protect, Systemp Desensitizer, Seal & Protect, Aqua-Prep F, Isodan, Gluma, BisBlock, D/Sense Crystal, UltraEZ, Colgate Sensitive Pro-Relief, Topex, and Clinpro White Varnish) on cultured human gingival- and pulp-derived fibroblast cells were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test (Serva, Heidelberg, Germany) under two different conditions. In the first test, different dilutions of the DDPs were directly applied onto cultured gingival fibroblast cells, and in the second test, the products were applied onto different-thickness dentin discs (0.5 and 1 mm) placed above cell culture medium, which contained pulp fibroblast cells.
  
  
  RESULTS
  According to the cytotoxicity evaluations of gingival fibroblast cells, the cytotoxicity of all of the DDPs was very high at 50% concentrations (p<0.05). Colgate Sensitive Pro-Relief, Clinpro White Varnish, and Topex showed higher cytotoxicity than did the other products (p<0.05), decreasing with further dilutions, and these products were found to be less cytotoxic to both types of cells (p<0.05) than were the other products with further dilutions. The cytotoxicity to human gingival and pulpal fibroblast cells of Systemp Desensitizer, Aqua-Prep F, Isodan, and Gluma did not show any decrease with further dilutions, and these products were found to be more cytotoxic than the other products (p<0.05).
  
  
  CONCLUSIONS
  According to the findings of this study, Colgate Sensitive Pro-Relief, Topex, and Clinpro White Varnish were less cytotoxic than the other DDPs used in this study.
authors.forename: G B
authors.initials: GB
authors.lastname: Eyüboğlu
authors.forename: C
authors.initials: C
authors.lastname: Yeşilyurt
authors.forename: M
authors.initials: M
authors.lastname: Ertürk
mesh_terms: D003764:Dental Materials; D003804:Dentin; D057133:Dentin Desensitizing Agents / Q000494:pharmacology*; D003806:Dentin Permeability / Q000187:drug effects; D003807:Dentin Sensitivity; D004338:Drug Combinations; D005881:Gingiva / Q000187:drug effects; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology; D009566:Nitrates; D011109:Polymethacrylic Acids / Q000494:pharmacology; D017680:Potassium Compounds; D012969:Sodium Fluoride
chemical_list: C438504:Aqua-Prep F; D003764:Dental Materials; D057133:Dentin Desensitizing Agents; D004338:Drug Combinations; C473099:Isodan; D008689:Methacrylates; D009566:Nitrates; D011109:Polymethacrylic Acids; D017680:Potassium Compounds; C489334:Systemp.desensitizer; C489164:UltraEZ; D012969:Sodium Fluoride; C044372:Gluma; D005976:Glutaral