title: Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface.
abstract: We have discovered that an electrode containing a conical channel with a small angular divergence can transmit into the vacuum almost 100% of an electrospray ion current produced at atmospheric pressure. Our first implementation of such a conical duct, which we term "ConDuct," uses a conductive plastic pipette tip containing an approximately 1.6° divergent channel at its entrance. We observed that the beam formed by the ConDuct electrode has a very low divergence (less than 1°) and persists for long distances in vacuum. Intrigued by these properties, we incorporated this electrode into a novel atmosphere-to-vacuum ion transmission interface, and devised a technique for evaluating its performance relative to the commercial reference interfaces that contain heated metal capillaries. We determined that our new interface transmits at least 400 times more ions than the commercial Thermo LCQ DECA XP atmosphere-to-vacuum interface and 2 to 3 times more than the commercial interface in the Thermo Velos Orbitrap and the Q Exactive mass spectrometers. We conclude that it might be possible to optimize the properties of the transmitted ions further by manufacturing ConDuct inlet electrodes from metal rather than conductive plastic and by determining the optimum angle of channel divergence and channel length.
authors.affiliation: Laboratory for Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY, 10065, USA.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Krutchinsky
authors.forename: Júlio C
authors.initials: JC
authors.lastname: Padovan
authors.forename: Herbert
authors.initials: H
authors.lastname: Cohen
authors.forename: Brian T
authors.initials: BT
authors.lastname: Chait
mesh_terms: D001274:Atmospheric Pressure; D004566:Electrodes; D004867:Equipment Design; D007477:Ions / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D014618:Vacuum
chemical_list: D007477:Ions; D010446:Peptide Fragments