title: A new FRET ratiometric fluorescent chemosensor for Hg²⁺ and its application in living EC 109 cells.
abstract: On the basis of fluorescent resonance energy transfer, a new fluorophore dyad (L) bearing rhodamine B and naphthalimide was developed as fluorescent ratiometric chemosensor for Hg(2+) in aqueous solution. L exhibited high selectivity and excellent sensitivity towards Hg(2+) with a broad pH span (1.0-8.0) and the detection limit of L was 2.11×10(-8) M. Sensor L for the detection of Hg(2+) was rapid and the recognizing event could complete in 2.5 min. A significant change in the color could be used for naked-eye detection. The selective fluorescence response of L to Hg(2+) is due to the Hg(2+)-promoted ring opening of spirolactam of rhodamine moiety, leading to a cyclization reaction of thiourea moiety. In addition, fluorescence imaging experiments of Hg(2+) in living EC 109 cells demonstrated its value of practical applications in biological systems.
authors.affiliation: Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Clinical Medicine, Zhengzhou University, Zhengzhou, China.
authors.forename: Manxiu
authors.initials: M
authors.lastname: Huai
authors.affiliation: Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Wang
authors.affiliation: Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China; Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, China.
authors.forename: Yufen
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Phosphorus Chemical Engineering Research Center of Henan Province, The College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China; Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, China. Electronic address: yeyong@zzu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D003116:Color; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D008628:Mercury / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D008628:Mercury
keywords: Biological systems; FRET; Fluorescence; Hg(2+); Ratiometric