title: Oncogenic Role of the Ec Peptide of the IGF-1Ec Isoform in Prostate Cancer.
abstract: IGF-1 is one of the key molecules in cancer biology; however, little is known about the role of the preferential expression of the premature IGF-1 isoforms in prostate cancer. We have examined the role of the cleaved COO- terminal peptide (PEc) of the third IGF-1 isoform, IGF-1Ec, in prostate cancer. Our evidence suggests that endogenously produced PEc induces cellular proliferation in the human prostate cancer cells (PC-3) in vitro and in vivo, by activating the ERK1/2 pathway in an autocrine/paracrine manner. PEc overexpressing cells and tumors presented evidence of epithelial to mesenchymal transition, whereas the orthotopic injection of PEc-overexpressing, normal prostate epithelium cells (HPrEC) in SCID mice was associated with increased metastatic rate. In humans, the IGF-1Ec expression was detected in prostate cancer biopsies, where its expression correlates with tumor stage. Our data describes the action of PEc in prostate cancer biology and defines its potential role in tumor growth, progression and metastasis.
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Armakolas
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Kaparelou
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Andreas
authors.initials: A
authors.lastname: Dimakakos
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Efstathia
authors.initials: E
authors.lastname: Papageorgiou
authors.affiliation: Third Orthopaedic Clinic, KAT General Hospital, Kifisia, Attiki, Greece.
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Armakolas
authors.affiliation: Third Orthopaedic Clinic, KAT General Hospital, Kifisia, Attiki, Greece.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Antonopoulos
authors.affiliation: Department of Pathology, Metropolitan General Hospital, Athens, Greece.
authors.forename: Constantina
authors.initials: C
authors.lastname: Petraki
authors.affiliation: Department of Pathology, Metropolitan General Hospital, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Lekarakou
authors.affiliation: Biomedical Research Foundation Academy of Athens, Center for Experimental Surgery, Athens, Greece.
authors.forename: Pavlos
authors.initials: P
authors.lastname: Lelovas
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Martha
authors.initials: M
authors.lastname: Stathaki
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Constantinos
authors.initials: C
authors.lastname: Psarros
authors.affiliation: Laboratory for Research of the Musculoskeletal System Theodoros Garofalidis, University of Athens, KAT Hospital Kifisia, Attiki, Greece.
authors.forename: Ismene
authors.initials: I
authors.lastname: Donta
authors.affiliation: Molecular Carcinogenesis Group, Laboratory of Histology and Embryology, Medical School, University of Athens, Greece.
authors.forename: Panos S
authors.initials: PS
authors.lastname: Galanos
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Paul
authors.initials: P
authors.lastname: Msaouel
authors.affiliation: Molecular Carcinogenesis Group, Laboratory of Histology and Embryology, Medical School, University of Athens, Greece.|Biomedical Research Foundation, Academy of Athens, Athens, Greece.|Institute for Cancer Sciences, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.|Manchester Centre for Cellular Metabolism, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
authors.forename: Vassilis G
authors.initials: VG
authors.lastname: Gorgoulis
authors.affiliation: Physiology Laboratory, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece.
authors.forename: Michael
authors.initials: M
authors.lastname: Koutsilieris
mesh_terms: D000818:Animals; D019898:Autocrine Communication; D001706:Biopsy; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D058750:Epithelial-Mesenchymal Transition; D015870:Gene Expression; D064593:Heterografts; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008954:Models, Biological; D009362:Neoplasm Metastasis; D019899:Paracrine Communication; D010455:Peptides / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020033:Protein Isoforms
chemical_list: D010455:Peptides; D020033:Protein Isoforms; D007334:Insulin-Like Growth Factor I