title: Three new species of Trichoderma with hyaline ascospores from China.
abstract: Collections of Trichoderma having hyaline ascospores from different areas of China were examined. Using combined analyses of morphological data, culture characters and phylogenetic information based on rDNA sequences of partial nuc translation elongation factor 1-α encoding gene (TEF1-α) and the gene encoding the second largest nuc RNA polymerase subunit (RPB2), three new species, Trichoderma applanatum, T. oligosporum and T. sinoluteum, were discovered and are described. Trichoderma applanatum produces continuous flat to pulvinate, white to cream stromata with dense orange or pale brown ostioles, and simple acremonium-like to verticillium-like conidiophores, belongs to the Hypocreanum clade and is closely related to T. decipiens. Trichoderma oligosporum forms reddish brown stromata with a downy surface, hyaline conidia and gliocladium-like conidiophores, and is closely related to but distinct from T. crystalligenum in the Psychrophila clade. Trichoderma sinoluteum, as a member of the Polysporum clade, is characterized by pale yellow stromata, white pustulate conidiomata, pachybasium-like conidiophores, and hyaline conidia. Differences between the new species and their close relatives are discussed.
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China University of Chinese Academy of Sciences, Beijing 100049, P.R. China zhuangwy@im.ac.cn.
authors.forename: Z X
authors.initials: ZX
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R. China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Zhuang
mesh_terms: D002681:China; D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D013172:Spores, Fungal / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D014242:Trichoderma / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005656:Fungal Proteins; D020648:Peptide Elongation Factor 1; D012321:DNA-Directed RNA Polymerases
keywords: Hypocreales; RPB2; TEF1-α; phylogeny; sequence analyses; taxonomy