title: Photosensitive GFP mutants containing an azobenzene unnatural amino acid.
abstract: The incorporation of unnatural amino acids represents a unique mechanism for the modulation of protein function. This approach has been utilized to generate photoswitchable GFP mutants, capable of demonstrating modulated fluorescence upon exposure to UV irradiation. Overall these photosensitive GFP mutants can be employed in various biosensing and diagnostic techniques to better understand protein function and processing.
authors.affiliation: Department of Chemistry, College of William & Mary, Williamsburg, VA 23187, USA.
authors.forename: Marshall S
authors.initials: MS
authors.lastname: Padilla
authors.affiliation: Department of Chemistry, College of William & Mary, Williamsburg, VA 23187, USA.
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Young
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000378:metabolism*; D001391:Azo Compounds / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014466:Ultraviolet Rays
chemical_list: D000596:Amino Acids; D001391:Azo Compounds; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins; C009850:azobenzene
keywords: Aminoacyl-tRNA synthetase; Fluorescent sensors; Green fluorescent protein; Protein expression; Unnatural amino acids