title: Oral Delivery of Bovine Lactoferrin Using Pectin- and Chitosan-Modified Liposomes and Solid Lipid Particles: Improvement of Stability of Lactoferrin.
abstract: A critical problem associated with delivery of bovine lactoferrin (bLf) by the oral route is low bioavailability, which is derived from the enzymatic degradation in the gastrointestinal tract and poor permeation across the intestinal epitheliums. Particulate carrier systems have been identified to protect bLf against proteolysis via encapsulation. This study aimed to evaluate the physico-chemical stability of bLf-loaded liposomes and solid lipid particles (SLPs) modified by pectin and chitosan when exposed to various stress conditions. Transmission electron microscopy results showed liposomes and SLPs had a classic shell-core structure with polymer layers surrounded on surface, but the structure appeared to be partially broken after digestion in simulated intestinal fluid (SIF). Although HPLC and sodium dodecyl sulphate-polyacrylamide gel electrophoresis methods qualitatively and quantitatively described either liposomes or SLPs could retain intact bLf against proteolysis in SIF to some extent, all liposome formulations showed rapid rate of lipolysis mediated by pancreatic enzymes. On the other hand, all SLP formulations showed higher heat resistance and greater electrolyte tolerance compared to liposome formulations. After 180 days storage time, liposome-loaded bLf was completely degraded, whereas almost 30% of intact bLf still remained in SLP formulations. Overall, SLPs are considered as primary choice for oral bLf delivery.
authors.affiliation: School of Pharmacy, Faculty of Medical and Health Science, The University of Auckland, Auckland, 1142, New Zealand.
authors.forename: Xudong
authors.initials: X
authors.lastname: Yao
authors.affiliation: Faculty of Agriculture and Life Science, Lincoln University, Lincoln, 7647, New Zealand.
authors.forename: Craig
authors.initials: C
authors.lastname: Bunt
authors.affiliation: School of Medicine, Faculty of Medical and Health Science, The University of Auckland, Auckland, 1142, New Zealand.
authors.forename: Jillian
authors.initials: J
authors.lastname: Cornish
authors.affiliation: School of Chemical Science, The University of Auckland, Auckland, 1142, New Zealand.
authors.forename: Siew-Young
authors.initials: SY
authors.lastname: Quek
authors.affiliation: School of Pharmacy, Faculty of Medical and Health Science, The University of Auckland, Auckland, 1142, New Zealand.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Wen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002417:Cattle; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D007781:Lactoferrin / Q000737:chemistry* / Q000494:pharmacology; D008081:Liposomes; D010368:Pectins / Q000737:chemistry* / Q000494:pharmacology; D055550:Protein Stability
chemical_list: D008081:Liposomes; D010368:Pectins; D048271:Chitosan; D007781:Lactoferrin
keywords: Drug Delivery/ADMET; Nanotechnology (Drug Discovery); Protein Structure