title: Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats.
abstract: OBJECTIVE
  The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency.
  
  
  METHODS
  Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC).
  
  
  RESULTS
  All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency.
  
  
  CONCLUSIONS
  The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane.
authors.affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Tábata de Mello
authors.initials: Tde M
authors.lastname: Tera
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Rodrigo Dias
authors.initials: RD
authors.lastname: Nascimento
authors.affiliation: Department of Bioscience and Oral Diagnosis, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Renata Falchete do
authors.initials: RF
authors.lastname: Prado
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Mauro Pedrine
authors.initials: MP
authors.lastname: Santamaria
authors.affiliation: Department of Diagnosis and Surgery, Instituto de Ciência e Tecnologia, Univ. Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Maria Aparecida Neves
authors.initials: MA
authors.lastname: Jardini
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods*; D004967:Estrogens / Q000172:deficiency; D005260:Female; D048091:Guided Tissue Regeneration / Q000379:methods*; D007150:Immunohistochemistry; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D008334:Mandible / Q000601:surgery; D010006:Osteoblasts / Q000502:physiology; D015675:Osteocalcin / Q000032:analysis; D015676:Osteonectin / Q000032:analysis; D010024:Osteoporosis / Q000503:physiopathology; D010052:Ovariectomy; D011138:Polytetrafluoroethylene / Q000627:therapeutic use*; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D004967:Estrogens; D058574:Integrin-Binding Sialoprotein; D015676:Osteonectin; D015675:Osteocalcin; D011138:Polytetrafluoroethylene