title: Simultaneous Estimation of Withaferin A and Z-Guggulsterone in Marketed Formulation by RP-HPLC.
abstract: A simple, rapid, precise and accurate high-performance liquid chromatography (HPLC) method was developed for simultaneous estimation of withaferin A and Z-guggulsterone in a polyherbal formulation containing Withania somnifera and Commiphora wightii. The chromatographic separation was achieved on a Purosphere RP-18 column (particle size 5 µm) with a mobile phase consisting of Solvent A (acetonitrile) and Solvent B (water) with the following gradients: 0-7 min, 50% A in B; 7-9 min, 50-80% A in B; 9-20 min, 80% A in B at a flow rate of 1 mL/min and detection at 235 nm. The marker compounds were well separated on the chromatogram within 20 min. The results obtained indicate accuracy and reliability of the developed simultaneous HPLC method for the quantification of withaferin A and Z-guggulsterone. The proposed method was found to be reproducible, specific, precise and accurate for simultaneous estimation of these marker compounds in a combined dosage form. The HPLC method was appropriate and the two markers are well resolved, enabling efficient quantitative analysis of withaferin A and Z-guggulsterone. The method can be successively used for quantitative analysis of these two marker constituents in combination of marketed polyherbal formulation.
authors.affiliation: Medicinal Natural Products Research Laboratory (MNPRL), Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (E), Mumbai 400 019, India pasagrawal@gmail.com.
authors.forename: Poonam
authors.initials: P
authors.lastname: Agrawal
authors.affiliation: Medicinal Natural Products Research Laboratory (MNPRL), Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (E), Mumbai 400 019, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Vegda
authors.affiliation: Medicinal Natural Products Research Laboratory (MNPRL), Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga (E), Mumbai 400 019, India.
authors.forename: Kirti
authors.initials: K
authors.lastname: Laddha
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D029744:Commiphora / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D028321:Plant Preparations / Q000737:chemistry*; D011282:Pregnenediones / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D032341:Withania / Q000737:chemistry; D054358:Withanolides / Q000032:analysis* / Q000737:chemistry
chemical_list: D028321:Plant Preparations; D011282:Pregnenediones; D054358:Withanolides; C023617:pregna-4,17-diene-3,16-dione; C009684:withaferin A