title: An inexpensive, fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen.
abstract: We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.
authors.affiliation: Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@uwe.ac.uk (J.M.B.); patrickwraith@blueyonder.co.uk (P.W.); janice.kiely@uwe.ac.uk (J.K.); hp@hphawkins.com (P.H.).
authors.forename: Jacqueline M
authors.initials: JM
authors.lastname: Barnett
authors.affiliation: Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@uwe.ac.uk (J.M.B.); patrickwraith@blueyonder.co.uk (P.W.); janice.kiely@uwe.ac.uk (J.K.); hp@hphawkins.com (P.H.).
authors.forename: Patrick
authors.initials: P
authors.lastname: Wraith
authors.affiliation: Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@uwe.ac.uk (J.M.B.); patrickwraith@blueyonder.co.uk (P.W.); janice.kiely@uwe.ac.uk (J.K.); hp@hphawkins.com (P.H.).
authors.forename: Janice
authors.initials: J
authors.lastname: Kiely
authors.affiliation: Bristol Urological Institute, Southmead Hospital, Bristol, BS10 5NB, UK; E-Mail: rajpersad@bristolurology.com.
authors.forename: Raj
authors.initials: R
authors.lastname: Persad
authors.affiliation: Department of Surgery, University Hospitals Bristol NHS Trust, Upper Maudlin Street, Bristol, BS2 8HW, UK; E-Mail: Katrina.Hurley@uhbristol.nhs.uk.
authors.forename: Katrina
authors.initials: K
authors.lastname: Hurley
authors.affiliation: Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@uwe.ac.uk (J.M.B.); patrickwraith@blueyonder.co.uk (P.W.); janice.kiely@uwe.ac.uk (J.K.); hp@hphawkins.com (P.H.).
authors.forename: Peter
authors.initials: P
authors.lastname: Hawkins
authors.affiliation: Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK; E-Mails: jackie.barnett@uwe.ac.uk (J.M.B.); patrickwraith@blueyonder.co.uk (P.W.); janice.kiely@uwe.ac.uk (J.K.); hp@hphawkins.com (P.H.).
authors.forename: Richard
authors.initials: R
authors.lastname: Luxton
keywords: paramagnetic particles; prostate specific antigen; resonant coil magnetometer