title: Isolation and purification of two antioxidant peptides from alcalase hydrolysate of Arca subcrenata.
abstract: OBJECTIVE
  To investigate the constituents with antioxidant activities from alcalase hydrolysate of Arca subcrenata.
  
  
  METHODS
  The consecutive chromatographic methods were employed,including ion-exchange chromatography, gel filtration chromatography, and reverse phase high-performance liquid chromatography (RP-HPLC). The amino acid sequences of the purified antioxidant peptides were determined by automated Edman degradation.
  
  
  RESULTS
  Under the guidance of the assay of scavenging free radicals, two peptides with antioxidant activities, termed as A-Bg1 and A-Bh, were isolated and purified from the alcalase hydrolysate of Arca subcrenata.
  
  
  CONCLUSIONS
  Constituents from the hydrolysate of Arca subcrenata might be a new potential resource of antioxidants.
authors.affiliation: Guangdong Food and Drug Vocational-technical School, Guangzhou, China.
authors.forename: Ting-Fei
authors.initials: TF
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Song
authors.forename: Rong-Min
authors.initials: RM
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000975:Antioxidants / Q000302:isolation & purification*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D056148:Chromatography, Reverse-Phase; D010455:Peptides / Q000302:isolation & purification*; D013381:Subtilisins
chemical_list: D000975:Antioxidants; D010455:Peptides; D013381:Subtilisins