title: Molecularly imprinted polymer based on MWCNT-QDs as fluorescent biomimetic sensor for specific recognition of target protein.
abstract: A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer speed with a response time of 25min. It is found that the BSA as a target protein can significantly quench the luminescence of BMIP-coated MWCNT-QDs in a concentration-dependent manner that is best described by a Stern-Volmer equation. The KSV for BSA is much higher than bovine hemoglobin and lysozyme, implying a highly selective recognition of the BMIP-coated MWCNT-QDs to BSA. Under optimal conditions, the relative fluorescence intensity of BMIP-coated MWCNT-QDs decreases linearly with the increasing target protein BSA in the concentration range of 5.0×10(-7)-35.0×10(-7)M with a detection limit of 80nM.
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Zhaoqiang
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of Life Sciences, Faculty of Science and Technology, University of Westminster, 115 New Cavendish Street, London W1W 6UW, UK.
authors.forename: S W
authors.initials: SW
authors.lastname: Annie Bligh
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tao
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Quan
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: niehuali@dhu.edu.cn.
authors.forename: Huali
authors.initials: H
authors.lastname: Nie
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China. Electronic address: lzhu@dhu.edu.cn.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Gong
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D002417:Cattle; D006454:Hemoglobins / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D006454:Hemoglobins; D037742:Nanotubes, Carbon; D012710:Serum Albumin, Bovine; D009113:Muramidase
keywords: Fluorescent biomimetic sensor; Molecularly imprinted polymer; Multi-walled carbon nanotubes; Protein recognition; Quantum dots