title: Genotyping of CCR5 gene, CCR2b and SDF1 variants related to HIV-1 infection in Gabonese subjects.
abstract: OBJECTIVE
  Given the magnitude of the HIV epidemic infection, many viral and human factors were analyzed, and the most decisive was the variant CCR5-Δ32. The presence of a low HIV prevalence (1.8%) in Gabon in the 1990s, compared to neighboring countries, represents a paradox that led us to search for viral and human genetic variants in this country. In this study, only variants of coreceptors and chemokines were investigated.
  
  
  METHODS
  Variants of the coding region of the CCR5 gene were analyzed by denaturing gradient gel electrophoresis, and then variants of SDF1 and CCR2b were determined by polymerase chain reaction-restriction fragment length polymorphism.
  
  
  RESULTS
  Four rare variants of the CCR5 coreceptor were found, while CCR5-Δ32 and CCR5m303 variants were not found. No association with CCR2b-V64I (17%) and SDF1-3'A (2%) variants was determined in relation to HIV-1 infection in Gabonese patients.
  
  
  CONCLUSIONS
  The paradox of HIV seroprevalence in Gabon, which ended in the 2000s, was not caused by human genetic variants but rather by environmental factors.
authors.affiliation: Centre International de Recherches Médicales de Franceville (CIRMF), Université des Sciences et Techniques de Masuku (USTM), Franceville, Gabon.
authors.forename: Landry Erik
authors.initials: LE
authors.lastname: Mombo
authors.forename: Cyrille
authors.initials: C
authors.lastname: Bisseye
authors.forename: Patrick
authors.initials: P
authors.lastname: Mickala
authors.forename: Simon
authors.initials: S
authors.lastname: Ossari
authors.forename: Maria
authors.initials: M
authors.lastname: Makuwa
mesh_terms: D054377:Chemokine CXCL12 / Q000235:genetics*; D058645:Denaturing Gradient Gel Electrophoresis; D004777:Environment; D005681:Gabon / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000453:epidemiology* / Q000235:genetics; D015528:HIV Seroprevalence; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D054390:Receptors, CCR2 / Q000235:genetics*; D019713:Receptors, CCR5 / Q000235:genetics*
chemical_list: C515334:CCR2 protein, human; C584933:CCR5 protein, human; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D054390:Receptors, CCR2; D019713:Receptors, CCR5