title: Redox state of flavin adenine dinucleotide drives substrate binding and product release in Escherichia coli succinate dehydrogenase.
abstract: The Complex II family of enzymes, comprising respiratory succinate dehydrogenases and fumarate reductases, catalyzes reversible interconversion of succinate and fumarate. In contrast to the covalent flavin adenine dinucleotide (FAD) cofactor assembled in these enzymes, soluble fumarate reductases (e.g., those from Shewanella frigidimarina) that assemble a noncovalent FAD cannot catalyze succinate oxidation but retain the ability to reduce fumarate. In this study, an SdhA-H45A variant that eliminates the site of the 8α-N3-histidyl covalent linkage between the protein and FAD was examined. Variants SdhA-R286A/K/Y and -H242A/Y that target residues thought to be important for substrate binding and catalysis were also studied. The variants SdhA-H45A and -R286A/K/Y resulted in the assembly of a noncovalent FAD cofactor, which led to a significant decrease (-87 mV or more) in its reduction potential. The variant enzymes were studied by electron paramagnetic resonance spectroscopy following stand-alone reduction and potentiometric titrations. The "free" and "occupied" states of the active site were linked to the reduced and oxidized states of FAD, respectively. Our data allow for a proposed model of succinate oxidation that is consistent with tunnel diode effects observed in the succinate dehydrogenase enzyme and a preference for fumarate reduction catalysis in fumarate reductase homologues that assemble a noncovalent FAD.
authors.affiliation: Department of Biochemistry, University of Alberta , Edmonton, Alberta T6G 2H7, Canada.
authors.forename: Victor W T
authors.initials: VW
authors.lastname: Cheng
authors.forename: Ramanaguru Siva
authors.initials: RS
authors.lastname: Piragasam
authors.forename: Richard A
authors.initials: RA
authors.lastname: Rothery
authors.forename: Elena
authors.initials: E
authors.lastname: Maklashina
authors.forename: Gary
authors.initials: G
authors.lastname: Cecchini
authors.forename: Joel H
authors.initials: JH
authors.lastname: Weiner
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism*; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D013379:Substrate Specificity / Q000502:physiology; D013385:Succinate Dehydrogenase / Q000737:chemistry / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D005182:Flavin-Adenine Dinucleotide; D013385:Succinate Dehydrogenase