title: The effects of panaxadiol saponins on megakaryocytic maturation and immune function in a mouse model of immune thrombocytopenia.
abstract: We have identified a biologically active component, panaxadiol saponins component (PDS-C), from Chinese ginseng herb extract. Panaxadiol saponins component contains five ginsenoside monomers with total purity of 92.44%. In this study, the BALB/c mouse model with immune thrombocytopenia (ITP) was established by injection of antiplatelet antibody every other day for 5 total times; the peripheral blood platelet counts steadily decreased to 20%-30% of normal levels and remained decreased for about 10 days. The antiplatelet antibody was derived from the sera of guinea pigs immunized with the platelets of BALB/c mice. Mice with ITP were treated with PDS-C at a low, a moderate, or a high dose for 10 consecutive days. We observed that the peripheral blood platelet counts of ITP mice were significantly higher than that of ITP controls (untreated) after treatment of PDS-C in a dose-dependent manner. Treatment with PDS-C also increased the mature megakaryocytes in the bone marrow of treated ITP animals with a concomitant decease of immature megakaryocyte precursors. Furthermore, macrophage phagocytosis of exogenous erythrocytes in the intra-abdominal cavity of ITP mice was inhibited by PDS-C treatment, indicating that PDS-C also could modulate immune function and may possibly prevent phagocytosis of antibody-coated platelets. Altogether, our findings suggest that PDS-C may have a dual role, promoting proliferation and differentiation of megakaryocytes, as well as modulating immune function, and it may therefore be very helpful in the treatment of ITP.
authors.affiliation: Institute of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Lin
authors.affiliation: Institute of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Yin
authors.affiliation: Institute of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China. Electronic address: gaoruilan@126.com.
authors.forename: Ruilan
authors.initials: R
authors.lastname: Gao
authors.affiliation: Institute of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Hematology Research, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Haematology, St George Hospital and St George Clinical School, University of New South Wales, Sydney, Australia.
authors.forename: Xingmai
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Haematology, St George Hospital and St George Clinical School, University of New South Wales, Sydney, Australia.
authors.forename: Beng Hock
authors.initials: BH
authors.lastname: Chong
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D005260:Female; D036145:Ginsenosides / Q000494:pharmacology*; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008533:Megakaryocytes / Q000187:drug effects* / Q000276:immunology; D008807:Mice, Inbred BALB C; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D010976:Platelet Count; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000188:drug therapy* / Q000276:immunology; D012503:Saponins / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D036145:Ginsenosides; D012503:Saponins; C004564:panaxadiol