title: Bioaugmentation of half-matured granular sludge with special microbial culture promoted establishment of 2,4-dichlorophenoxyacetic acid degrading aerobic granules.
abstract: Aerobic granular sludge degrading recalcitrant compounds are generally hard to be cultivated. This study investigated the feasibility of cultivating 2,4-dichlorophenoxyacetic acid (2,4-D) degrading aerobic granules using half-matured sludge granules pre-grown on glucose as the seeds and bioaugmentation with a 2,4-D degrading strain Achromobacter sp. QXH. Results showed that bioaugmentation promoted the steady transformation of glucose-grown granules to 2,4-D degrading sludge granules and fast establishment of 2,4-D degradation ability. The 2,4-D degradation rate of the bioaugmented granules was enhanced by 36-62 % compared to the control at 2,4-D concentrations of 144-565 mg/L on Day 18. The inoculated strain was incorporated into the half-matured granules successfully and survived till the end of operation (220 days). Sludge granules at a mean size of 420 µm and capable of utilizing 500 mg/L 2,4-D as the sole carbon source were finally obtained. Sludge microbial community shifted slightly during the whole operation and the dominant bacteria species belonged to Proteobacteria.
authors.affiliation: Key Laboratory of Water and Sediment Sciences of Ministry of Education/State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, No.19, Xinjiekouwai Street, Haidian District, Beijing, 100875, People's Republic of China, xchquan@bnu.edu.cn.
authors.forename: Xiangchun
authors.initials: X
authors.lastname: Quan
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
authors.forename: Weicong
authors.initials: W
authors.lastname: Xiong
authors.forename: Xinrui
authors.initials: X
authors.lastname: Wang
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000378:metabolism*; D000332:Aerobiosis; D019149:Bioreactors; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage; D015084:2,4-Dichlorophenoxyacetic Acid