title: Technologies to probe functions and mechanisms of long noncoding RNAs.
abstract: Thousands of long noncoding RNAs (lncRNAs) have been discovered, but their functional characterization has been slowed by a limited set of research tools. Here we review emerging RNA-centric methods to interrogate the intrinsic structure of lncRNAs as well as their genomic localization and biochemical partners. Understanding these technologies, including their advantages and caveats, and developing them in the future will be essential to progress from description to comprehension of the myriad roles of lncRNAs.
authors.affiliation: Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Ci
authors.initials: C
authors.lastname: Chu
authors.affiliation: 1] Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA. [2] Department of Pharmaceutical Sciences, University of California at Irvine, Irvine, California, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Spitale
authors.affiliation: 1] Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA. [2] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
authors.forename: Howard Y
authors.initials: HY
authors.lastname: Chang
mesh_terms: D008967:Molecular Biology / Q000379:methods*; D062085:RNA, Long Noncoding / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*
chemical_list: D062085:RNA, Long Noncoding