title: The effects of a single bout pilates exercise on mRNA expression of bone metabolic cytokines in osteopenia women.
abstract: OBJECTIVE
  The purpose of this study was to examine the effect of a single bout pilates exercise on mRNA expression of bone metabolic cytokines in elderly osteopenia women.
  
  
  METHODS
  We selected 11 people of elderly osteopenia women and loaded a single bout pilates exercise about RPE 10-14 level. The blood samples were collected before, immediately after and 60 minute after pilates exercise, then examined calcium metabolic markers in serum and extracted peripheral blood mononuclear cell (PBMC) from whole blood and confirmed mRNA expression of bone metabolic cytokines from PBMC. To clarify the changes during exercise, we designed repeated measure ANOVA as the control group to perform blood sampling without exercise.
  
  
  RESULTS
  As a result, serum P showed significant interaction effect between group and time (p<.001), the pilates exercise group decreased about 9% at immediately after exercise and 13% during recovery after exercise (p<.05), while the control group showed a tendency to increase. Serum CK also showed a significant interaction between group and time (p<.05), the pilates group significantly increased at immediately after exercise and during recovery after exercise (p<.05) but the control group didn't have changes. TNF-α and IL-6 mRNA expression in PBMC was significantly increased in the pilates group (p<.01, p<.05), although INF-γ mRNA expression didn't show statistically significant difference, it tended to increase in the pilates group (NS).
  
  
  CONCLUSIONS
  These results suggested that a single bout pilates exercise of elderly osteopenia women cause hypophosphatemia with temporary muscle damage, and it leading high turnover bone metabolic state with to activate both of bone formation and bone resorption.
authors.affiliation: Department of Physical Education, Dongduk Women's University, Seoul, Korea.
authors.forename: Chang Sun
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Department of Physical Education, Dongduk Women's University, Seoul, Korea.
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Physical Education, Dongduk Women's University, Seoul, Korea.
authors.forename: Hyo Jin
authors.initials: HJ
authors.lastname: Kim
keywords: Hypophosphatemia.; IL-6; INF-γ; PBMC; Pilates exercise; TNF-α; mRNA expression