title: Mannose receptor mediated phagocytosis of bacteria in macrophages of blunt snout bream (Megalobrama amblycephala) in a Ca(2+)-dependent manner.
abstract: Mannose receptor (MR) is an important pattern-recognition receptor in macrophages and plays a critical role in immune responses. It is has been reported that mammalian macrophages are able to engulf a wide range of microorganisms mediated by Ca(2+)-dependent MR binding to terminal mannose residues which are frequently found on the pathogen surfaces. However, little is known about the MR-mediated phagocytosis in macrophages of fish. In this report, the distributions of MR in the macrophage and head kidney tissue from blunt snout bream were examined using MaMR specific antibody generated in our lab. Mannan and MaMR specific antibody inhibition experiments results collectively showed that MR was involved in the GFP-expressed E. coli engulfed in the macrophages, resulting in respiratory burst, nitric oxide production as well as inflammatory cytokines secretion, and the MaMR-mediated phagocytosis was Ca(2+)-dependent. These results will shed a new light on the immune functions of teleost MRs.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Lichun
authors.initials: L
authors.lastname: Liu
authors.affiliation: Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), 13621, Egypt.
authors.forename: Abeer M
authors.initials: AM
authors.lastname: Hegazy
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Biological Sciences, Lake Superior State University, Sault Ste. Marie 49783, MI, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China. Electronic address: liuxl@mail.hzau.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan, Hubei 430070, China; Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan, Hubei 430070, China. Electronic address: linli@mail.hzau.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D003530:Cyprinidae / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D060226:Head Kidney / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D009928:Organ Specificity; D010587:Phagocytosis; D011956:Receptors, Cell Surface / Q000378:metabolism*; D016897:Respiratory Burst
chemical_list: D016207:Cytokines; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface; D009569:Nitric Oxide; D002118:Calcium
keywords: Bacteria; Macrophage; Mannose receptor; Megalobrama amblycephala; Phagocytosis