title: Light-induced protein dimerization by one- and two-photon activation of gibberellic acid derivatives in living cells.
abstract: We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA3 ). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
authors.affiliation: Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg (Germany); InnovationLab GmbH, Speyerer Strasse 4, 69115 Heidelberg (Germany).
authors.forename: Korwin M
authors.initials: KM
authors.lastname: Schelkle
authors.forename: Tristan
authors.initials: T
authors.lastname: Griesbaum
authors.forename: Dirk
authors.initials: D
authors.lastname: Ollech
authors.forename: Steffy
authors.initials: S
authors.lastname: Becht
authors.forename: Tiago
authors.initials: T
authors.lastname: Buckup
authors.forename: Manuel
authors.initials: M
authors.lastname: Hamburger
authors.forename: Richard
authors.initials: R
authors.lastname: Wombacher
mesh_terms: D000818:Animals; D019556:COS Cells; D002470:Cell Survival / Q000528:radiation effects; D002522:Chlorocebus aethiops; D005875:Gibberellins / Q000737:chemistry / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D008027:Light; D008968:Molecular Conformation; D017785:Photons; D055503:Protein Multimerization / Q000528:radiation effects*
chemical_list: D005875:Gibberellins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C007842:gibberellic acid
keywords: caged compounds; chemical inducer of dimerization; photolysis; protein-protein interactions; two-photon activation