title: [Study on the genotype and epidemic characteristics of diarrheagenic Escherichia coli isolated from diarrheal patients in Beijing].
abstract: OBJECTIVE
  To understand the distribution of virulence gene and the epidemiological characteristics of diarrheagenic Escherichia(E.) coli (DEC) from diarrheal patients in Beijing.
  
  
  METHODS
  Stool specimens from diarrheal patients were cultured which were collected from the hospitals under sentinel surveillance program, during 2012-2013. DNA was examined by real-time PCR.
  
  
  RESULTS
  253 out of 6 370 specimens were positive for DEC detection with the rate as 4.0%. A total number of 262 DEC strains were isolated. Two different pathotypes of DEC strains with mixed infection, were isolated from 9 specimens. Different pathotypes would show the following profiles: 42.8% for enteropathogenic E. coli (EPEC) including 42.0% atypical and 0.8% typical; 38.9% for enterotoxigenic E. coli (ETEC) including 24.8% st positive, 9.9% lt positive and 4.2% st and lt both positive;15.3% for enteroaggregative E. coli(EAEC);2.7% for enteroinvasive E. coli (EIEC); one strain STEC with serotype O26:K60. ETEC had obvious characteristics on age. All kinds of DEC were isolated throughout the year with seasonal fluctuation.
  
  
  CONCLUSIONS
  DEC isolates from diarrheal patients in Beijing were dominated by EPEC and ETEC, with atypical ones accounted for the majority of EPEC. One specimen was found under mixed infection. Pathotypes DEC were found to have different age and seasonal distributions.
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qu
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Haikun
authors.initials: H
authors.lastname: Qian
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Lyu
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Hanqiu
authors.initials: H
authors.lastname: Yan
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, Beijing 100013, China. Email: wangqy@bjcdc.org.
authors.forename: Quanyi
authors.initials: Q
authors.lastname: Wang
mesh_terms: D002681:China / Q000453:epidemiology; D003967:Diarrhea / Q000382:microbiology*; D054308:Enteropathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D058872:Epidemics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000453:epidemiology* / Q000382:microbiology*; D005838:Genotype; D006801:Humans; D014774:Virulence