title: DNA methylation abnormalities in congenital heart disease.
abstract: Congenital heart defects represent the most common malformation at birth, occurring also in ∼50% of individuals with Down syndrome. Congenital heart defects are thought to have multifactorial etiology, but the main causes are largely unknown. We have explored the global methylation profile of fetal heart DNA in comparison to blood DNA from control subjects: an absolute correlation with the type of tissue was detected. Pathway analysis revealed a significant enrichment of differential methylation at genes related to muscle contraction and cardiomyopathies in the developing heart DNA. We have also searched for abnormal methylation profiles on developing heart-tissue DNA of syndromic and non-syndromic congenital heart defects. On average, 3 regions with aberrant methylation were detected per sample and 18 regions were found differentially methylated between groups. Several epimutations were detected in candidate genes involved in growth regulation, apoptosis and folate pathway. A likely pathogenic hypermethylation of several intragenic sites at the MSX1 gene, involved in outflow tract morphogenesis, was found in a fetus with isolated heart malformation. In addition, hypermethylation of the GATA4 gene was present in fetuses with Down syndrome with or without congenital heart defects, as well as in fetuses with isolated heart malformations. Expression deregulation of the abnormally methylated genes was detected. Our data indicate that epigenetic alterations of relevant genes are present in developing heart DNA in fetuses with both isolated and syndromic heart malformations. These epimutations likely contribute to the pathogenesis of the malformation by cis-acting effects on gene expression.
authors.affiliation: a Department of Experimental and Health Sciences ; Universitat Pompeu Fabra ; Barcelona , Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Serra-Juhé
authors.forename: Ivon
authors.initials: I
authors.lastname: Cuscó
authors.forename: Aïda
authors.initials: A
authors.lastname: Homs
authors.forename: Raquel
authors.initials: R
authors.lastname: Flores
authors.forename: Núria
authors.initials: N
authors.lastname: Torán
authors.forename: Luis A
authors.initials: LA
authors.lastname: Pérez-Jurado
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004247:DNA / Q000097:blood / Q000378:metabolism; D019175:DNA Methylation; D005260:Female; D005333:Fetus / Q000378:metabolism; D055106:Genome-Wide Association Study; D006330:Heart Defects, Congenital / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000378:metabolism; D011247:Pregnancy; D055815:Young Adult
chemical_list: D004247:DNA
keywords: CHD, congenital heart defects; DMCpGs, differentially methylated CpGs; DNA methylation; DS, Down syndrome; Down syndrome; MS-MLPA, Methylation Specific Multiple Ligation-Dependent Probe Amplification; TRR, transcriptional regulatory region; congenital heart disease; epimutation; heart malformation; iCHD, isolated CHD; qRT-PCR, quantitative real-time PCR