title: Post-hypoxic and ischemic neuroprotection of BMP-7 in the cerebral cortex and caudate-putamen tissue of rat.
abstract: Previous reports have indicated that exogenous bone morphogenetic protein-7 (BMP-7) has a neuroprotective effect after cerebral ischemia injury and promotes motor function recovery, but the appropriate BMP-7 concentration and time course are unclear. Here, we assessed endogenous BMP-7 expression in hypoxia and ischemia-damaged brain tissues and investigated the effects of different BMP-7 concentrations in pre- and post-hypoxic primary rat neurons. The results showed that BMP-7 expression was significantly higher in the ischemic hemisphere. The expressions of BMP-7 and caspase-3 were localized in the cytoplasm of the primary cerebral cortical and caudate-putamen neurons 24h after hypoxia/reoxygenation. After BMP-7 treatment, the number of caspase-3 positive neurons began to decrease with increasing BMP-7 concentrations up to 80ng/ml, but not beyond. Although the numbers of caspase-3-positive neurons between pre- and post-hypoxia/reoxygenation were not significantly different, more dendrites were observed in the groups treated prior to hypoxia/reoxygenation. These results suggest that increased BMP-7 expression can be induced in the cerebral cortex and caudate-putamen both in vivo and in vitro in hypoxic-ischemic states. The neuroprotective mechanism of BMP-7 may include apoptosis suppression, and its effect was enhanced from 40 to 80ng/ml. Pre-hypoxic BMP-7 treatment may be useful to stimulate dendrite sprouting in non-injured neurons.
authors.affiliation: Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Liju
authors.initials: L
authors.lastname: Luan
authors.affiliation: Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Changman
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Anatomy and Histoembryology, School of Basic Medical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China. Electronic address: qinlihua88@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Qin
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000096:biosynthesis*; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis; D002421:Caudate Nucleus / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D008297:Male; D011699:Putamen / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; C505410:Casp3 protein, rat; D053148:Caspase 3
keywords: Bone morphogenetic protein-7; Caudate–putamen; Cerebral cortex; Hypoxia; Ischemia; Rat