title: A Rapid Centrifugation-Assisted Solid-Phase Extraction and Liquid Chromatography Method for Determination of Loureirin A and Loureirin B of Dragon's Blood Capsules in Rat Plasma and Urine After Oral Administration.
abstract: A simple, sensitive and rapid centrifugation-assisted solid-phase extraction (SPE) with high-performance liquid chromatography (SPE-HPLC) method was developed for simultaneous determination of the metabolites loureirin A and loureirin B from Dragon's blood in rat plasma and urine. The development of the extraction procedure included optimization of some important extraction phases. After evaluation, the metabolites of Dragon's blood were extracted by centrifugation-assisted SPE and separated by using HPLC. This method showed good linearity (r(2) > 0.99), and in the rat plasma and urine, the recoveries were 93.1 and 95.7% for loureirin A and were 90.1 and 94.2% for loureirin B. The relative standard deviation (RSD) values of intraday and interday precision in rat plasma and urine for loureirin A were <3.84 and 2.01%, respectively. The RSD values of the intraday and interday precision in rat plasma and urine for loureirin B were below 4.25 and 5.83%, respectively. Thus, the established method is suitable for metabolism studies of loureirin A and loureirin B in rat plasma and urine.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, P.R. China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, 650118 Kunming, P.R. China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Li
authors.affiliation: Qingdao Huanghai Pharmaceutical Co., Ltd. of Shandong Province, 266000 Qingdao, P.R. China.
authors.forename: Shangfang
authors.initials: S
authors.lastname: Ma
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, 650500 Kunming, P.R. China huxjia@gmail.com.
authors.forename: Xujia
authors.initials: X
authors.lastname: Hu
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002498:Centrifugation / Q000379:methods*; D047188:Chalcones / Q000097:blood* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D016014:Linear Models; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D051381:Rats; D015203:Reproducibility of Results; D012116:Resins, Plant / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D047188:Chalcones; D010936:Plant Extracts; D012116:Resins, Plant; C560420:loureirin A; C525191:dragon's blood; C505764:loureirin B