title: FGF-23 levels in patients with critical carotid artery stenosis.
abstract: The aim of this study was to evaluate fibroblast growth factor (FGF)-23 serum levels and its tissue expression in patients with critical carotid artery stenosis (CAS). We selected 35 patients with critical CAS undergoing carotid thromboendoarterectomy. In each patient, FGF-23 serum levels were evaluated just prior to the surgery (t0) and 30 min (t1) thereafter. Moreover, macrophage cytokines were measured at baselines. Carotid artery specimens were used for immune histochemistry. On the basis of the histology, the patients were divided into 2 groups: A with complicated plaque and B with uncomplicated plaque. Twenty complicated plaques (57.14%, group A,) and 15 uncomplicated (42.86%, group B) were evaluated: calcifications were present in 16/20 (80%) complicated plaques and in 6/15 (40%) uncomplicated plaques. An inflammatory infiltrate was observed in 26/35 carotid samples: 18/26 (69.23%) complicated and 8/26 (30.76%) uncomplicated. FGF-23(+) cells were present in 17/20 complicated (85%) and in 8 uncomplicated (53%) plaques. The double-staining immunofluorescence confirmed that macrophage cells (CD68(+)) were also positive for FGF-23 staining. Serum levels of FGF-23 were significantly higher in group A versus group B at t0 (p < 0.05) and t1 (p 0.0047). Moreover, in group A patients a significant increase of FGF-23 serum levels was observed at t1 in comparison with t0 (p 0.0011). Our results suggest that FGF-23 acts in the late phases of atherosclerotic disease and may potentially represent a marker of complications in critical CAS.
authors.affiliation: Dipartimento di Medicina Clinica e Molecolare, Facoltà di Medicina e Psicologia, UOC Medicina 3, Azienda Ospedaliera Sant'Andrea, Università "La Sapienza", Rome, Italy, flavia.delporto@uniroma1.it.
authors.forename: Flavia
authors.initials: F
authors.lastname: Del Porto
authors.forename: Maria
authors.initials: M
authors.lastname: Proietta
authors.forename: Cira
authors.initials: C
authors.lastname: di Gioia
authors.forename: Noemi
authors.initials: N
authors.lastname: Cifani
authors.forename: Raffaele
authors.initials: R
authors.lastname: Dito
authors.forename: Cristiano
authors.initials: C
authors.lastname: Fantozzi
authors.forename: Livia
authors.initials: L
authors.lastname: Ferri
authors.forename: Lucrezia
authors.initials: L
authors.lastname: Fabriani
authors.forename: Michele
authors.initials: M
authors.lastname: Rossi
authors.forename: Luigi
authors.initials: L
authors.lastname: Tritapepe
authors.forename: Maurizio
authors.initials: M
authors.lastname: Taurino
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D016893:Carotid Stenosis / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D016022:Case-Control Studies; D016894:Endarterectomy, Carotid; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D012307:Risk Factors
chemical_list: D015415:Biomarkers; C000717427:FGF23 protein, human; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23