title: Determination of dansylated amino acids and biogenic amines in Cannonau and Vermentino wines by HPLC-FLD.
abstract: Free amino acids (AA) and biogenic amines (BA) were quantified for the first time in Cannonau and Vermentino wines, the two most popular "Controlled Designation of Origin" wines from Sardinia (Italy). An analytical method for the simultaneous determination of AA and BA was developed, using selective derivatization with dansyl chloride followed by HPLC with fluorescence detection. Thirty-two compounds were identified in the wines analysed. High levels of AA were found, with proline being the most abundant with average levels of 1244 ± 398 and 1008 ± 281 mg/L in Cannonau and Vermentino wines, respectively. BA were detected at average concentrations <10mg/L, except putrescine which reached 20.5 ± 10.2mg/L in Cannonau wines. Histamine was never detected in any Vermentino wines. γ-Aminobutyric acid, 4-hydroxyproline, glycine, leucine+isoleucine and putrescine proved to be useful for differentiating Cannonau wines from Vermentino wines.
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy. Electronic address: tuberoso@unica.it.
authors.forename: Carlo Ignazio Giovanni
authors.initials: CI
authors.lastname: Tuberoso
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Congiu
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Serreli
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mameli
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D001679:Biogenic Amines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000032:analysis*; D007558:Italy; D014920:Wine / Q000032:analysis*
chemical_list: D000596:Amino Acids; D001679:Biogenic Amines; D003619:Dansyl Compounds; C029038:dansyl chloride
keywords: Amino acids; Biogenic amines; Dansyl chloride; Fluorescence detection; HPLC; Validation; Wine