title: Anethole, a Medicinal Plant Compound, Decreases the Production of Pro-Inflammatory TNF-α and IL-1β in a Rat Model of LPS-Induced Periodontitis.
abstract: Periodontitis (PD) is known to be one of most prevalent worldwide chronic inflammatory diseases. There are several treatments including antibiotics for PD; however, since drug resistance is an increasing problem, new drugs particularly derived from plants with fewer side effects are required. The effects of trans-anethole on IL-1 β and TNF-α level in a rat model of PD were investigated and compared to ketoprofen. Eschericia coli lipopolysaccharide (LPS, 30 µg) was injected bilaterally into the palatal gingiva (3 µL/site) between the upper first and second molars every two days for 10 days in anesthetized rats. Administration of either trans-anethole (10 or 50 mg/Kg, i.p.) or ketoprofen (10 mg/Kg, i.p.) was started 20 minute before LPS injection and continued for 10 days. Then, IL-1β and TNF-α levels were measured in blood samples by ELISA at day 0 (control) and at day 10. Anethole at both concentrations significantly suppressed IL-1β and TNF-α production when compared to LPS-treated rats. The suppressive effects of anethole on LPS-induced pro-inflammatory cytokines were almost similar as seen with ketoprofen. In conclusion, the present results suggest that anethole may have a potent inhibitory effect on PD through suppression of pro-inflammatory molecules; therefore it could be a novel therapeutic strategy for PD.
authors.affiliation: Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Janet
authors.initials: J
authors.lastname: Moradi
authors.affiliation: Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran. ; Dental Faculty, Semnan Unversity of Medical Sciences, Senman, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Abbasipour
authors.affiliation: Neurophysiology Research Centre (NPRC) and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran.
authors.forename: Jalal
authors.initials: J
authors.lastname: Zaringhalam
authors.affiliation: Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Bita
authors.initials: B
authors.lastname: Maleki
authors.affiliation: Department of Periodontics, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Ziaee
authors.affiliation: Private Practice in Periodontics and Implant Surgery, Hamadan, Iran.
authors.forename: Amin
authors.initials: A
authors.lastname: Khodadoustan
authors.affiliation: Neurophysiology Research Centre (NPRC) and Department of Physiology, Medical School, Shahid Beheshti University of Medical Sciences, Evin, Tehran, Iran.
authors.forename: Mahyar
authors.initials: M
authors.lastname: Janahmadi
keywords: Anti-inflammatory; IL-1β; Ketoprofen; LPS-induced periodontitis; TNF-α; Trans-anethole