title: Behavior of osteoblasts on TI surface with two different coating designed for orthodontic devices.
abstract: In the present study we coated Ti surfaces with polytetrafluorethylene (PTFE) and titanium nitride (TiN) and investigated in vitro the behavior of osteoblasts on these surfaces. MG-63 osteoblasts were cultured on titanium discs with different surface treatment: uncoated Ti6Al4V, TiN-coated, PTFE-coated. Cell viability/proliferation was detected by MTT assay. Gene-expression levels of alkaline phosphatase (ALP), osteocalcin (OC), type I collagen, receptor activator of nuclear factor-kappa-B ligand (RANKL), and osteoprotegerin (OPG) were determined by qPCR. Cell behavior on different surfaces was observed by time-lapse microscopy. Cells grown on PTFE-coated Ti surface exhibited delayed surface attachment and decreased proliferation after 48 h. However, after 168 h of culture cells grown on PTFE-coated surface exhibited higher viability/proliferation, higher expression levels of ALP and OC, and higher OPG/RANKL ratio compared to uncoated surface. No effect of TiN-coating on any investigated parameter was found. Our results shows that PTFE coating exhibits no toxic effect on MG-63 cells and slightly stimulates expression of several genes associated with osteogenesis. We propose that PTFE coating could be considered as a possible choice for a surface treatment of temporary skeletal anchorage devices in orthodontics.
authors.affiliation: Division of Oral Biology, Bernhard Gottlieb School of Dentistry, Medical University of Vienna, Vienna, Austria.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Fleischmann
authors.forename: Adriano
authors.initials: A
authors.lastname: Crismani
authors.forename: Frank
authors.initials: F
authors.lastname: Falkensammer
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Bantleon
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Rausch-Fan
authors.forename: Oleh
authors.initials: O
authors.lastname: Andrukhov
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D020099:Coated Materials, Biocompatible; D015870:Gene Expression; D006801:Humans; D009967:Orthodontic Appliances; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D014025:Titanium