title: Oleanane triterpenes from the flowers of Camellia japonica inhibit porcine epidemic diarrhea virus (PEDV) replication.
abstract: Porcine epidemic diarrhea virus (PEDV) infections have resulted in a severe economic loss in the swine industry in many countries due to no effective treatment approach. Fifteen oleanane triterpenes (1-15), including nine new ones (1-4 and 10-14), were isolated from the flowers of Camellia japonica, and their molecular structures were determined by extensive spectroscopic methods. These compounds were evaluated for their antiviral activity against PEDV replication, and the structure-activity relationships (SARs) were discussed. Compounds 6, 9, 11, and 13 showed most potent inhibitory effects on PEDV replication. They were found to inhibit PEDV genes encoding GP6 nucleocapsid, GP2 spike, and GP5 membrane protein synthesis based on RT-PCR data. Western blot analysis also demonstrated their inhibitory effects on PEDV GP6 nucleocapsid and GP2 spike protein synthesis during viral replication. The present study suggested the potential of compounds 6, 9, 11, and 13 as promising scaffolds for treating PEDV infection via inhibiting viral replication.
authors.affiliation: Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University , Sillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
authors.forename: Jun-Li
authors.initials: JL
authors.lastname: Yang
authors.forename: Thi-Kim-Quy
authors.initials: TK
authors.lastname: Ha
authors.forename: Basanta
authors.initials: B
authors.lastname: Dhodary
authors.forename: Euisun
authors.initials: E
authors.lastname: Pyo
authors.forename: Ngoc Hieu
authors.initials: NH
authors.lastname: Nguyen
authors.forename: Hyomoon
authors.initials: H
authors.lastname: Cho
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.forename: Won Keun
authors.initials: WK
authors.lastname: Oh
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D028244:Camellia / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D035264:Flowers / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D053485:Porcine epidemic diarrhea virus / Q000187:drug effects* / Q000254:growth & development*; D013329:Structure-Activity Relationship; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; C413246:oleanane; D009828:Oleanolic Acid