title: Impact of fish oils on the outcomes of a mouse model of acute Pseudomonas aeruginosa pulmonary infection.
abstract: Pseudomonas aeruginosa is an opportunistic Gram-negative bacterium that causes pneumonia in immunocompromised humans and severe pulmonary damage in patients with cystic fibrosis. Imbalanced fatty acid incorporation in membranes, including increased arachidonic acid and decreased DHA concentrations, is known to play a critical role in chronic inflammation associated with bacterial infection. Other lipids, such as EPA and alkylglycerols, are also known to play a role in inflammation, particularly by stimulating the immune system, decreasing inflammation and inhibiting bacterial growth. In this context, the goal of the present study was to assess the effect of dietary DHA/EPA, in a 2:1 ratio, and alkylglycerols, as natural compounds extracted from oils of rays and chimeras, respectively, on the inflammatory reaction induced by P. aeruginosa pulmonary infection in mice. To this end, mice were fed with a control diet or isolipidic, isoenergetic diets prepared with oils enriched in DHA/EPA (2:1) or alkylglycerols for 5 weeks before the induction of acute P. aeruginosa lung infection by endotracheal instillation. In our model, DHA/EPA (2:1) significantly improved the survival of mice after infection, which was associated with the acceleration of bacterial clearance and the resolution of inflammation leading to the improvement of pulmonary injuries. By contrast, alkylglycerols did not affect the outcomes of P. aeruginosa infection. Our findings suggest that supplementation with ray oil enriched in DHA/EPA (2:1) can be considered as a preventive treatment for patients at risk for P. aeruginosa infection.
authors.affiliation: Inserm U995, Team 4, IFR 114, University Lille Nord de France,Lille59045,France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Caron
authors.affiliation: Inserm U995, Team 4, IFR 114, University Lille Nord de France,Lille59045,France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Desseyn
authors.affiliation: Copalis,Boulogne-sur-Mer62203,France.
authors.forename: Luce
authors.initials: L
authors.lastname: Sergent
authors.affiliation: Nutricia Research,Uppsalalaan 12,3584CTUtrecht,The Netherlands.
authors.forename: Nana
authors.initials: N
authors.lastname: Bartke
authors.affiliation: Inserm U995, Team 4, IFR 114, University Lille Nord de France,Lille59045,France.
authors.forename: Marie-Odile
authors.initials: MO
authors.lastname: Husson
authors.affiliation: Unit of Biostatistics, CERIM, EA2694, UDSL, University Lille Nord de France,Lille,France.
authors.forename: Alain
authors.initials: A
authors.lastname: Duhamel
authors.affiliation: Inserm U995, Team 4, IFR 114, University Lille Nord de France,Lille59045,France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Gottrand
mesh_terms: D000818:Animals; D058491:Bacterial Load; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use; D005395:Fish Oils / Q000627:therapeutic use*; D005399:Fishes; D007113:Immunity, Innate; D018928:Immunity, Mucosal; D007155:Immunologic Factors / Q000627:therapeutic use; D008099:Liver / Q000737:chemistry*; D008168:Lung / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D008297:Male; D008810:Mice, Inbred C57BL; D020556:Neutrophil Infiltration / Q000276:immunology; D010539:Permeability; D018410:Pneumonia, Bacterial / Q000178:diet therapy / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D011552:Pseudomonas Infections / Q000178:diet therapy / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000276:immunology* / Q000302:isolation & purification; D011897:Random Allocation; D020545:Respiratory Mucosa / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016019:Survival Analysis
chemical_list: D016207:Cytokines; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D007155:Immunologic Factors
keywords: Alkylglycerols; Inflammation; Pseudomonas aeruginosa infection; n-3 Long-chain PUFA