title: Association between sHLA-G and HLA-G 14-bp deletion/insertion polymorphism in Crohn's disease.
abstract: The aim of this study was to evaluate the association between the HLA-G 14-bp deletion/insertion (Del/Ins) polymorphism and soluble (s) HLA-G production in patients with Crohn's disease (CD). We analyzed also the sHLA-G molecules by ELISA and western blot in plasma samples. Among unselected patients, the 14-bp Del/Ins polymorphism was not significantly associated with increased CD risk neither for alleles (P = 0.371) nor for genotypes (P = 0.625). However, a significant association was reported between the 14-bp Del/Ins polymorphism and CD, in particular in young-onset CD patients for alleles [P = 0.020, odds ratio (OR) = 2.438, 95% confidence interval (CI): 1.13-5.25] but not with adult-onset CD patients. A significant association was reported concerning the genotype Ins/Ins for young-onset CD patients (P = 0.029, OR = 3.257, 95% CI: 1.08-9.77). We observed also a significant increase in sHLA-G measured by ELISA in CD patients compared to controls (P = 0.002). The 14-bp Del/Del and 14-bp Del/Ins genotypes are the high HLA-G producers. Among sHLA-G(positive) patients, 43% of subjects present dimers of HLA-G. The presence of dimers seems to be related to the advanced stages of the disease. The 14-bp Del/Ins polymorphism is associated with an increased risk of CD particularly in young-onset CD patients and controls sHLA-G plasma levels. Dimers of sHLA-G are frequent in advanced disease stages. The above findings indicate that the genetic 14-bp Del/Ins polymorphism in exon 8 of the HLA-G gene is associated with the risk of CD and suggest a role for sHLA-G as a prognostic marker for progressive disease.
authors.affiliation: Department of Biology, Laboratoire Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia ines.zidi@techemail.com.
authors.forename: Inès
authors.initials: I
authors.lastname: Zidi
authors.affiliation: Department of Biology, Laboratoire Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Hamza
authors.initials: H
authors.lastname: Ben Yahia
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Daria
authors.initials: D
authors.lastname: Bortolotti
authors.affiliation: Department of Biology, Laboratoire Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia Gastroenterology Department, Charles Nicolle Hospital, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Leila
authors.initials: L
authors.lastname: Mouelhi
authors.affiliation: Department of Biology, Laboratoire Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia Laboratory of Microbiology-Immunology, UR02SP13, CHU Farhat Hached, Sousse, Tunisia.
authors.forename: Ahmed Baligh
authors.initials: AB
authors.lastname: Laaribi
authors.affiliation: Gastroenterology Department, Charles Nicolle Hospital, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Shema
authors.initials: S
authors.lastname: Ayadi
authors.affiliation: Faculty of Medicine Ibn Al Jazzar, University of Sousse, Sousse, Tunisia.
authors.forename: Nour
authors.initials: N
authors.lastname: Zidi
authors.affiliation: Gastroenterology Department, Charles Nicolle Hospital, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Houissa
authors.affiliation: Gastroenterology Department, Charles Nicolle Hospital, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Radhouane
authors.initials: R
authors.lastname: Debbech
authors.affiliation: Department of Biology, Laboratoire Microorganismes et Biomolécules Actives, Sciences Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Abdellatif
authors.initials: A
authors.lastname: Boudabous
authors.affiliation: Gastroenterology Department, Charles Nicolle Hospital, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis, Tunisia.
authors.forename: Taoufik
authors.initials: T
authors.lastname: Najjar
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Di Luca
authors.affiliation: Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Ferrara, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Rizzo
mesh_terms: D000328:Adult; D017668:Age of Onset; D003424:Crohn Disease / Q000235:genetics* / Q000276:immunology; D019281:Dimerization; D018450:Disease Progression; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059951:HLA-G Antigens / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D012306:Risk; D017384:Sequence Deletion; D055815:Young Adult
chemical_list: D059951:HLA-G Antigens
keywords: 14 bp; Crohn’s disease; HLA-G; conformation; polymorphism