title: [Determination of chlorogenic acid and luteoloside in lonicerae flos of different habitats and germplasms].
abstract: OBJECTIVE
  To establish an UPLC-PDA method for simultaneous determination of chlorogenic acid and luteoloside in Lonicerae Flos. On the basis of developed method, the quality of Lonicerae Flos from nine habitats and two local germplasms introduced from Qufu in Shandong to Wuming in Guangxi was evaluated.
  
  
  METHODS
  The analysis was performed on a Waters Acquity UPLC H-Class system. An Acquity UPLC BEH RP18 (100 mm x 2.1 mm,1.7 μm) column was used for all analyses. The investigated compounds were separated with a gradient mobile phase consisting of acetonitrile and 0.4% phosphoric acid solution at a flow rate of 0.2 mL/min, and the detection wavelength was set at 242 nm.
  
  
  RESULTS
  The quality of Lonicerae Flos from Qufu was the best among Lonicerae Flos of nine habitats for its content of chlorogenic acid and luteoloside at 35.715 and 1.270 mg/g, respectively. The content of chlorogenic acid and luteoloside in Lonicerae Flos of "Jiufengyihao" and "Shuxing" introduced from Qufu to Wuming both complied with the standard of Chinese Pharmacopoeia (2010 edition).
  
  
  CONCLUSIONS
  The developed UPLC-PDA method is simple, reliable and repeatable, which is helpful for the quality control of Lonicerae Flos. "Jiufengyihao" and "Shuxing" are potential germplasms for the introduction of Lonicerae Flos in Wuming.
authors.affiliation: Guangxi Botanical Garden of Medicinal Plants, Nanning, China. fenghuas@163.com
authors.forename: Feng-hua
authors.initials: FH
authors.lastname: Shi
authors.forename: Xiang-sheng
authors.initials: XS
authors.lastname: Zhao
authors.forename: Qiao-cheng
authors.initials: QC
authors.lastname: Mo
authors.forename: Li-wei
authors.initials: LW
authors.lastname: Xie
mesh_terms: D002681:China; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal; D017753:Ecosystem; D031239:Lonicera / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D002726:Chlorogenic Acid; C588038:lonicerae flos