title: Allergen expression in the European house dust mite Dermatophagoides pteronyssinus throughout development and response to environmental conditions.
abstract: House dust mites are a major source of allergy worldwide. While diagnosis and treatment based on mite extracts have remarkably advanced, little information exists on the expression of allergens in mites. We have studied gene expression of eight Dermatophagoides pteronyssinus (Trouessart) (Acari: Pyroglyphidae) allergens (Der p 1, 2, 3, 4, 5, 7, 10 and 21). All allergens showed higher transcription in nymphs compared with larvae or adults, with the only exception of Der p 10. The transcription of Der p 4 and Der p 10, together with the transcription and protein ratios Der p 1 to Der p 2, were higher in males than in females. One-week exposure of mite cultures to 16 or 35 °C (versus 24 °C) or low RH (44% versus 76%) significantly influenced the allergen gene transcription profile. Our results demonstrate that allergen expression is quantitatively and/or qualitatively influenced by mite development and sex, as well as by the environment. We suggest that monitoring allergen gene expression may be a useful tool to assist the optimization of mite cultures in the production of standardized allergenic extracts for clinical use.
authors.affiliation: Laboratorio de Interacción Planta-Insecto, Departamento de Biología Medioambiental, Centro de Investigaciones Biológicas - CSIC, Madrid, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Vidal-Quist
authors.forename: F
authors.initials: F
authors.lastname: Ortego
authors.forename: M
authors.initials: M
authors.lastname: Lombardero
authors.forename: P
authors.initials: P
authors.lastname: Castañera
authors.forename: P
authors.initials: P
authors.lastname: Hernández-Crespo
mesh_terms: D000818:Animals; D039741:Antigens, Dermatophagoides / Q000235:genetics* / Q000378:metabolism; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D040002:Dermatophagoides pteronyssinus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004777:Environment; D005260:Female; D005786:Gene Expression Regulation; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008297:Male; D009758:Nymph / Q000254:growth & development / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins
keywords: Allergen profile; ELISA; HDM; allergenic extracts; allergy; mite culture; real-time quantitative PCR; tropomyosin