title: [Correlation study on Chinese medical syndrome types of chronic hepatitis B patients and HLA-DR13 gene, BCP mutation, and T-lymphocyte subsets].
abstract: OBJECTIVE
  To explore the correlation between the HLA-DR13, basic core promoter (BCP), changes of T lymphocyte subset and clinical Chinese medical syndromes of chronic hepatitis B (CHB).
  
  
  METHODS
  Totally 102 CHB patients were syndrome typed as Gan depression Pi deficiency syndrome (GDPDS), Pi-Shen yang deficiency syndrome (PSYDS), Gan-gallbladder dampness heat syndrome (GGDHS), Gan-Shen yin deficiency syndrome (GSYDS), and static blood blocking collaterals syndrome (SBBCS). Besides, 30 healthy subjects were recruited as the normal control group. The blood HBV-DNA level and HLA-DR13 gene were detected with real time fluorescent PCR. The expression of CD4+ and CD8+ in T lymphocytes was detected using flow cytometry. The mutation of serum A1762T/G1764A was detected using PCR sequencing. Hepatitis Be antigen (HBeAg) was detected with ELISA, and correlation between various Chinese medical syndrome types and objective indicators were analyzed.
  
  
  RESULTS
  There was no statistical difference in HBV-DNA quantitative results among various syndrome types (P > 0.05). HBeAg positive rate was higher in GDPDS than in other syndrome types (P < 0.05). It was sequenced as GDPDS > GSYDS > SBBCS > GGDHS > PSYDS. Compared with the normal control group, percentages of CD3+ and CD3+ CD4+ were lower in PSYDS (P < 0.05). The ratio of CD3+ CD4+/CD3+ CD8 was lower in GGDHS and PSYDS than in the normal control group (P < 0.05). There was no statistical difference in the CD3+ CD8+ percentage among various syndrome types (P > 0.05). The quantitation of HLA-DR13 gene was lower in GDPDS and GSYDS than in the normal control group (P < 0.05). The positive rate of BCP mutation was higher in GSYDS than in other syndrome types (P < 0.05).
  
  
  CONCLUSIONS
  Co-detection results of HLA-DR13 and BCP could be used as reference indices of Chinese medical syndrome typing of CHB.
authors.affiliation: Department of Testing, First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. xiaorongy3196@qq.com
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Yang
authors.forename: Yin
authors.initials: Y
authors.lastname: Liu
authors.forename: Juan
authors.initials: J
authors.lastname: Ouyang
authors.forename: Xiu-Kun
authors.initials: XK
authors.lastname: Wang
authors.forename: Wei-Xin
authors.initials: WX
authors.lastname: Diao
mesh_terms: D059810:HLA-DR Serological Subtypes / Q000235:genetics / Q000378:metabolism; D019694:Hepatitis B, Chronic / Q000145:classification / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008516:Medicine, Chinese Traditional; D011401:Promoter Regions, Genetic; D013577:Syndrome; D016176:T-Lymphocyte Subsets / Q000378:metabolism; D016711:Yang Deficiency; D016710:Yin Deficiency
chemical_list: D059810:HLA-DR Serological Subtypes; C097337:HLA-DR13 antigen