title: ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin.
abstract: In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. zafar.hussain.ibupoto@liu.se.
authors.forename: Zafar Hussain
authors.initials: ZH
authors.lastname: Ibupoto
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. syeal@itn.liu.se.
authors.forename: Syed Muhammad Usman
authors.initials: SM
authors.lastname: Ali
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. kimleang.khun@liu.se.
authors.forename: Kimleang
authors.initials: K
authors.lastname: Khun
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. chan.oeurn.chey@liu.se.
authors.forename: Chan Oeurn
authors.initials: CO
authors.lastname: Chey
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. omeno@itn.liu.se.
authors.forename: Omer
authors.initials: O
authors.lastname: Nur
authors.affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. magnus.willander@itn.liu.se.
authors.forename: Magnus
authors.initials: M
authors.lastname: Willander