title: Lysosomal enzymes in serum and synovial fluid in patients with osteoarthritis.
abstract: OBJECTIVE
  To assess the activity of arylsulfatase (AS), acid phosphatase (ACP), cathepsin D (CAT-D) and alpha-1 antitrypsin (AAT) in blood serum and synovial fluid (SF) of patients with hip or knee osteoarthritis (OA).
  
  
  METHODS
  The study included 43 subjects with OA (35 hip OA, 8 knee OA), submitted total joint replacement. The control group consisted of 58 subjects with no past history of musculoskeletal disorders.
  
  
  RESULTS
  The OA blood serum samples showed a significantly higher level of lysosomal enzymes activity than in the control group (AS by 17.8%, AAT by 42.4%); only the CAT-D activity decreased by 50%). AS, ACP and CAT-D activities were about two-fold higher in SF when compared with blood of OA patients. The differences between the genders were visible in the SF: Total protein concentration, activity of ACP (both higher in OA men) and activity of CAT-D (higher in OA women). Between the involved hip and knee, there were no significant differences in all estimated parameters in the blood serum of the OA group. In regard to the SF, only ACP activity was significantly higher in patients with a hip involved.
  
  
  CONCLUSIONS
  The osteoarthritic SF enzymatic profile differs from that in normal joints. The OA in joints is not reflected in the systemic response. Our preliminary results suggest further studies on role of lysosomal enzymes (ACP and AS) as biomarkers for the detection of osteoarthritis.
authors.affiliation: Department of Pathobiochemistry and Clinical Chemistry, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun , Bydgoszcz , Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Olszewska-Slonina
authors.forename: Stanislaw
authors.initials: S
authors.lastname: Jung
authors.forename: Dariusz
authors.initials: D
authors.lastname: Matewski
authors.forename: Krzysztof J
authors.initials: KJ
authors.lastname: Olszewski
authors.forename: Ewa
authors.initials: E
authors.lastname: Krzyzynska-Malinowska
authors.forename: Andrzej
authors.initials: A
authors.lastname: Braszkiewicz
authors.forename: Bogna
authors.initials: B
authors.lastname: Kowaliszyn
mesh_terms: D000135:Acid Phosphatase / Q000097:blood / Q000378:metabolism; D000328:Adult; D000368:Aged; D001192:Arylsulfatases / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D002402:Cathepsin D / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D015207:Osteoarthritis, Hip / Q000097:blood / Q000201:enzymology*; D020370:Osteoarthritis, Knee / Q000097:blood / Q000201:enzymology*; D012016:Reference Values; D013582:Synovial Fluid / Q000378:metabolism*; D000515:alpha 1-Antitrypsin / Q000097:blood / Q000378:metabolism
chemical_list: D000515:alpha 1-Antitrypsin; D000135:Acid Phosphatase; D001192:Arylsulfatases; C533922:CTSD protein, human; D002402:Cathepsin D
keywords: Cathepsin D; acid phosphatase; alpha 1-antitrypsin; arylsulfatase; cartilage; osteoarthritis