title: Hypolipidemic Effect and Mechanism of Palmatine from Coptis chinensis in Hamsters Fed High-Fat diet.
abstract: Palmatine (PAL) is one of the main alkaloids in Coptis chinensis. The present aim was to investigate the hypolipidemic effect and mechanism of palmatine in hamsters fed with high-fat diet (HFD). PAL treatment decreased serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) levels, as well as increased fecal excretion of TC and total bile acids (TBA) in hyperlipidemic hamsters. Furthermore, PAL treatment up-regulated low-density lipoprotein receptor (LDLR) and cholesterol 7α-hydroxylase (CYP7A1) mRNA and protein expression and down-regulated apical sodium-dependent bile salt transporter (ASBT) mRNA and protein expression. These results demonstrated that PAL as a potential natural cholesterol lowering agent works by up-regulating LDLR and CYP7A1 mRNA and protein expression, down-regulating ASBT mRNA and protein expression, as well as enhancing fecal excretion of TC and TBA. The findings in our study suggest that palmatine could be a potential natural agent for treating hyperlipidemia.
authors.affiliation: School of Pharmaceutical Sciences, Southwest University, Chongqing, China; Engineer Research Center of Chongqing Pharmaceutical Process and Quality Control, Chongqing, China; Department of Pharmacy, Tongren Polytechnic College, Tongren, Guizhou, China.
authors.forename: Na
authors.initials: N
authors.lastname: Ning
authors.forename: Kai
authors.initials: K
authors.lastname: He
authors.forename: Yanzhi
authors.initials: Y
authors.lastname: Wang
authors.forename: Zongyao
authors.initials: Z
authors.lastname: Zou
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Xuegang
authors.initials: X
authors.lastname: Li
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ye
mesh_terms: D000818:Animals; D001600:Berberine Alkaloids / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002790:Cholesterol 7-alpha-Hydroxylase / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood; D039323:Coptis / Q000737:chemistry*; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D006949:Hyperlipidemias / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D008297:Male; D008647:Mesocricetus; D029363:Organic Anion Transporters, Sodium-Dependent / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011973:Receptors, LDL / Q000378:metabolism; D027981:Symporters / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015854:Up-Regulation
chemical_list: D001600:Berberine Alkaloids; D001647:Bile Acids and Salts; D008078:Cholesterol, LDL; D000960:Hypolipidemic Agents; D029363:Organic Anion Transporters, Sodium-Dependent; D012333:RNA, Messenger; D011973:Receptors, LDL; D027981:Symporters; D014280:Triglycerides; C071908:sodium-bile acid cotransporter; D002784:Cholesterol; D002790:Cholesterol 7-alpha-Hydroxylase; C005413:palmatine
keywords: ASBT; CYP7A1; LDLR; hyperlipidemia; hypolipidemic; palmatine