title: Anti-inflammatory activity of sappanchalcone isolated from Caesalpinia sappan L. in a collagen-induced arthritis mouse model.
abstract: Sappanchalcone, a bioactive flavonoid isolated from the heartwood of Caesalpinia sappan L. possesses anti-inflammatory effects. We studied the efficacy of sappanchalcone in attenuating collagen-induced arthritis (CIA) in a mouse model of rheumatoid arthritis. Sappanchalcone was purified to homogeneity from the chloroform fraction of the methanolic extract of C. sappan, and identified using mass spectrometry and (1)H-nuclear magnetic resonance spectroscopy. CIA-induced male DBA/1J mice were divided into control, sappanchalcone-treated, and methotrexate-treated groups (n = 10 per group). Paw swelling, arthritis severity, radiographic and histomorphometric changes were assessed to measure the protective role of sappanchalcone against chronic disease progression. Sappanchalcone administration significantly reduced clinical arthritis and inflammatory edema in paws. Bone mineral density and trabecular structure were maintained in CIA mice administered sappanchalcone. The levels of pro-inflammatory cytokines (TNF-α, IL-6, and 1L-1β) were significantly lower in the serum of sappanchalcone-treated mice as compared with the control group. Our results suggest that sappanchalcone could be used as an anti-inflammatory and bone-protective agent during the treatment of rheumatoid arthritis.
authors.affiliation: Department of Life Science and Biotechnology, Soonchunhyang University, Asan, Chungnam, 336-745, Republic of Korea.
authors.forename: Eui-Gil
authors.initials: EG
authors.lastname: Jung
authors.forename: Kook-Il
authors.initials: KI
authors.lastname: Han
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Bharat Bhusan
authors.initials: BB
authors.lastname: Patnaik
authors.forename: Wan-Jong
authors.initials: WJ
authors.lastname: Kim
authors.forename: Gang Min
authors.initials: GM
authors.lastname: Hur
authors.forename: Kung-Woo
authors.initials: KW
authors.lastname: Nam
authors.forename: Man-Deuk
authors.initials: MD
authors.lastname: Han
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification* / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000473:pathology; D029911:Caesalpinia / Q000737:chemistry*; D047188:Chalcones / Q000302:isolation & purification* / Q000494:pharmacology*; D003094:Collagen; D016207:Cytokines / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004798:Enzymes / Q000378:metabolism; D005528:Foot / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008811:Mice, Inbred DBA; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D047188:Chalcones; D016207:Cytokines; D004798:Enzymes; D010936:Plant Extracts; D003094:Collagen; C044363:sappanchalcone