title: Structural characterization of O- and C-glycosylating variants of the landomycin glycosyltransferase LanGT2.
abstract: The structures of the O-glycosyltransferase LanGT2 and the engineered, C-C bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates.
authors.affiliation: Institut für Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstrasse 21, 79104 Freiburg (Germany).
authors.forename: Heng Keat
authors.initials: HK
authors.lastname: Tam
authors.forename: Johannes
authors.initials: J
authors.lastname: Härle
authors.forename: Stefan
authors.initials: S
authors.lastname: Gerhardt
authors.forename: Jürgen
authors.initials: J
authors.lastname: Rohr
authors.forename: Guojun
authors.initials: G
authors.lastname: Wang
authors.forename: Jon S
authors.initials: JS
authors.lastname: Thorson
authors.forename: Aurélien
authors.initials: A
authors.lastname: Bigot
authors.forename: Monika
authors.initials: M
authors.lastname: Lutterbeck
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Seiche
authors.forename: Bernhard
authors.initials: B
authors.lastname: Breit
authors.forename: Andreas
authors.initials: A
authors.lastname: Bechthold
authors.forename: Oliver
authors.initials: O
authors.lastname: Einsle
mesh_terms: D018360:Crystallography, X-Ray; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry / Q000378:metabolism*; D062105:Molecular Docking Simulation; D011487:Protein Conformation; D015202:Protein Engineering
chemical_list: D016695:Glycosyltransferases
keywords: C-glycosylation; Friedel-Crafts alkylation; carbasugars; enzyme engineering; glycosyltransferases