title: Functionality study of Na6PMo11FeO40 as a mushroom tyrosinase inhibitor.
abstract: The inhibitory effects of iron-substituted phosphomolybdic acid (Na6PMo11FeO40, abbreviated as PMo11Fe) on mushroom tyrosinase were investigated. The Native-PAGE results show that PMo11Fe has an inhibitory effect on tyrosinase. A spectrophotometric analysis shows that PMo11Fe is a reversible and noncompetitive inhibitor with KI=KIS=0.47 mmol L(-1). The effects of PMo11Fe on the preservation of lotus root slices were studied. The results show that PMo11Fe can significantly slow the browning rate of lotus root slices, inhibit the activity of polyphenol oxidase (PPO), enhance the activity of peroxidase (POD) and superoxide dismutase (SOD), maintain high levels of glutathione (GSH), ascorbic acid (ASA) and sucrose and control the peroxidation degree of fresh-cut fruits and vegetables. This study may help to elucidate the design of mushroom tyrosinase inhibitors of polyoxometalates (abbreviated as POMs) and a theoretical basis for the use of POMs to inhibit fruit spoilage.
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Xing
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China.
authors.forename: Aping
authors.initials: A
authors.lastname: Zheng
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China. Electronic address: wanglimerry@jmu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Yu
authors.affiliation: College of Food and Bioengineering, Jimei University, Xiamen 361021, PR China; College of Chemistry and Life Science, Quanzhou Normal College, Quanzhou 362000, PR China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Jiang
mesh_terms: D000363:Agaricales / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005520:Food Preservatives / Q000494:pharmacology; D058085:Iron Compounds / Q000494:pharmacology*; D007700:Kinetics; D000070116:Lotus / Q000187:drug effects; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D010756:Phosphoric Acids / Q000737:chemistry / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods
chemical_list: D004791:Enzyme Inhibitors; D005520:Food Preservatives; D058085:Iron Compounds; D010756:Phosphoric Acids; D008982:Molybdenum; D014442:Monophenol Monooxygenase; C003125:phosphomolybdic acid
keywords: Ascorbic acid (PubChem CID: 54670067); Browning; Fresh-cut fruits and vegetables; Glutathione (PubChem CID: 124886); Phosphomolybdic acid (PubChem CID: 11251951); Polyoxometalates; Tyrosinase; l-3,4-Dihydroxyphenylalanine (PubChem CID: 6047); l-Tyrosine (PubChem CID: 6057)