title: PICT1 expression is a poor prognostic factor in non-small cell lung cancer.
abstract: PICT1 is a key regulator of the MDM2-TP53 pathway. High mRNA expression levels of PICT1 are associated with poor prognosis in several cancers with wild-type TP53. In this study, we identified the PICT1 protein expression profile in non-small cell lung cancer (NSCLC) with wild-type TP53 in the nucleolus and cytoplasm, and revealed the relationship between PICT1 expression and patient clinicopathological factors. PICT1 expression in the tumor cells of 96 NSCLC patients with wild-type TP53 was evaluated by immunohistochemistry. Forty-three of 96 (44.8%) NSCLC samples were positive for nucleolar PICT1, while 40/96 (41.7%) NSCLC samples were positive for cytoplasmic PICT1. There was no correlation between nucleolar PICT1 expression and clinicopathological factors. However, cytoplasmic PICT1 expression was significantly correlated with sex, smoking history, differentiation, lymphatic invasion and pathological stage. In multivariate analysis, lymphatic invasion was significantly associated with cytoplasmic PICT1 expression (hazard ratio: 5.02, P = 0.026). We scrutinized PICT1 expression in samples of NSCLC with wild-type TP53, and showed a correlation between cytoplasmic PICT1 expression and several clinicopathological factors in these patients. Our results indicate that cytoplasmic PICT1 expression is a poor prognostic factor and is associated with tumor progression via lymphatic invasion in these patients.
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Kyoko
authors.initials: K
authors.lastname: Okamura
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Takayama
authors.affiliation: Department of Molecular Oncology, Graduate School of Medical and Dental Science, Kagoshima University, Japan. ; Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Japan.
authors.forename: Kohichi
authors.initials: K
authors.lastname: Kawahara
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Taishi
authors.initials: T
authors.lastname: Harada
authors.affiliation: Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Nishio
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan. ; Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Otsubo
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan. ; Division of Pathophysiological and Experimental Pathology, Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Ijichi
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Mikihiro
authors.initials: M
authors.lastname: Kohno
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan. ; Faculty of Medical Sciences, Department of Comprehensive Clinical Oncology, Kyushu University, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Iwama
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Fujii
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ota
authors.affiliation: Division of Pathophysiological and Experimental Pathology, Department of Pathology, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Takaomi
authors.initials: T
authors.lastname: Koga
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Okamoto
authors.affiliation: Division of Cancer Genetics, Medical Institute of Bioregulation, Kyushu University, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Research Institute for Diseases of the Chest, Graduate School of Medical Sciences, Kyushu University, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
keywords: GLTSCR2; PICT1; TP53; lung cancer; lymphatic invasion