title: Increase in the expression of inducible nitric oxide synthase on exposure to bisphenol A: a possible cause for decline in steroidogenesis in male mice.
abstract: Bisphenol A (BPA) is a well-known plasticizer and xenoestrogen that is responsible for many acquired reproductive difficulties, especially in men. Despite the prevalence of BPA in society, the mechanism behind reproductive deficits remains elusive. The present study investigates the mode of BPA's action by evaluating its effect on the expression of inducible nitric oxide synthase (iNOS) and steriodogenic acute regulatoryprotein (StAR) in male mice testis. Swiss albino mice were treated with a range BPA concentrations of 0.5, 50 and 100μg/kg body weight/day intraperitoneally for 60 days. Several markers of oxidative stress and male fertility were investigated. Nitrite levels, malondialdehyde levels and testicular injury scores were elevated whereas the sperm count, serum testosterone levels and catalase activity were reduced in the BPA groups. Mechanistically, an increase in iNOS expression was observed in the testis whereas the expression of the StAR was down regulated in the BPA treated mouse. These results suggest that BPA induces oxidative stress by altering the expression of iNOS, which consequently leads to the down regulation of StAR expression in the testis of male mouse.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: shikhachouhan16@gmail.com.
authors.forename: Shikha
authors.initials: S
authors.lastname: Chouhan
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: satyndra_yadav@yahoo.co.in.
authors.forename: Satyndra Kumar
authors.initials: SK
authors.lastname: Yadav
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: jaiprakash_biotech@yahoo.co.in.
authors.forename: Jay
authors.initials: J
authors.lastname: Prakash
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: suswes00@gmail.com.
authors.forename: Susan
authors.initials: S
authors.lastname: Westfall
authors.affiliation: Department of Pathology, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: amrita_lko@rediffmail.com.
authors.forename: Amrita
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Department of Endocrinology and Metabolism, Institute of Medical Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: drnkavns@gmail.com.
authors.forename: Neeraj Kumar
authors.initials: NK
authors.lastname: Agarwal
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: suryasinghbhu16@gmail.com.
authors.forename: Surya Pratap
authors.initials: SP
authors.lastname: Singh
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D052244:Endocrine Disruptors / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D010636:Phenols / Q000633:toxicity*; D010750:Phosphoproteins / Q000378:metabolism*; D010968:Plasticizers / Q000633:toxicity*; D013076:Sperm Count; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000097:blood; D000098682:Steroidogenic Acute Regulatory Protein; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002374:Catalase; D052244:Endocrine Disruptors; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D009573:Nitrites; D010636:Phenols; D010750:Phosphoproteins; D010968:Plasticizers; D013739:Testosterone; D000098682:Steroidogenic Acute Regulatory Protein; C496317:Nos2 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Oxidative stress; Serum testosterone; Sperm count; StAR; iNOS