title: Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3.
abstract: The present study describes the gene cloning and high-level expression of an alkaline and thermostable lipase gene from Trichosporon coremiiforme V3. Nucleotide analysis revealed that this lipase gene has an open reading frame of 1,692 bp without any introns, encoding a protein of 563 amino acid residues. The lipase gene without its signal sequence was cloned into plasmid pPICZαA and overexpressed in Pichia pastoris X33. The maximum lipase activity of recombinant lipase was 5,000 U/ml, which was obtained in fed-batch cultivation after 168 h induction with methanol in a 50 L bioreactor. The purified lipase showed high temperature tolerance, and being stable at 60 °C and kept 45% enzyme activity after 1 h incubation at 70 °C. The stability, effects of metal ions and other reagents were also determined. The chain length specificity of the recombinant lipase showed high activity toward triolein (C18:1) and tripalmitin (C16:0).
authors.affiliation: Guangdong VTR Bio-Tech Co., Ltd, Zhuhai 519060, Guangdong, P.R. China.|Guangdong Feed Additive Research and Biotechnology Center, Zhuhai 519060, Guangdong, P.R. China.
authors.forename: Jian-Rong
authors.initials: JR
authors.lastname: Wang
authors.affiliation: Guangdong VTR Bio-Tech Co., Ltd, Zhuhai 519060, Guangdong, P.R. China.|Guangdong Feed Additive Research and Biotechnology Center, Zhuhai 519060, Guangdong, P.R. China.
authors.forename: Yang-Yuan
authors.initials: YY
authors.lastname: Li
authors.affiliation: Guangdong VTR Bio-Tech Co., Ltd, Zhuhai 519060, Guangdong, P.R. China.|Guangdong Feed Additive Research and Biotechnology Center, Zhuhai 519060, Guangdong, P.R. China.
authors.forename: Danni
authors.initials: D
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D003067:Coenzymes / Q000032:analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004795:Enzyme Stability; D015870:Gene Expression; D008049:Lipase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010843:Pichia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D013696:Temperature; D014250:Trichosporon / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D004271:DNA, Fungal; D008670:Metals; D011994:Recombinant Proteins; D008049:Lipase; C428386:thermostable lipase