title: Utilization of different waste proteins to create a novel PGPR-containing bio-organic fertilizer.
abstract: High-quality bio-organic fertilizers (BIOs) cannot be produced without the addition of some proteins, while many waste proteins are haphazardly disposed, causing serious environmental pollution. In this study, several waste proteins were used as additives to assist with the reproduction of the functional microbe (Bacillus amyloliquefaciens SQR9) inoculated into matured composts to produce BIOs. An optimized composition of solid-state fermentation (SSF) raw materials was predicted by response surface methodology and experimental validation. The results showed that 7.61% (w/w, DW, the same below) rapeseed meal, 8.85% expanded feather meal, 6.47% dewatered blue algal sludge and 77.07% chicken compost resulted in maximum biomass of strain SQR-9 and the maximum amount of lipopeptides 7 days after SSF. Spectroscopy experiments showed that the inner material structural changes in the novel SSF differed from the control and the novel BIO had higher dissolved organic matter. This study offers a high value-added utilization of waste proteins for producing economical but high-quality BIO.
authors.affiliation: Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: National Enginnering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Jianshu
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: 1] Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing. 210095, China [2] Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: 1] Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing. 210095, China [2] National Enginnering Research Center for Organic-based Fertilizers, Nanjing Agricultural University, Nanjing. 210095, China.
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001407:Bacillus; D018533:Biomass; D050260:Carbohydrate Metabolism; D002452:Cell Count; D002645:Chickens; D004553:Electric Conductivity; D005285:Fermentation; D005308:Fertilizers / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D055666:Lipopeptides / Q000378:metabolism; D008372:Manure; D009930:Organic Chemicals / Q000378:metabolism*; D063245:Plant Development; D011506:Proteins / Q000378:metabolism*; D012231:Rhizobium / Q000502:physiology*; D012987:Soil; D013050:Spectrometry, Fluorescence; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature
chemical_list: D005308:Fertilizers; D055666:Lipopeptides; D008372:Manure; D009930:Organic Chemicals; D011506:Proteins; D012987:Soil