title: Complex micropatterning of proteins within microfluidic channels.
abstract: Microfluidic channels containing protein micropatterned surfaces are useful in many bioanalytical and biological applications. In this study, we developed a new method to integrate microfluidics and protein micropatterning by attaching poly(dimethylsiloxane) (PDMS) microfluidic channels to bio-friendly photoresist films via poly(dopamine) (PDA) adhesive. A bio-friendly photoresist poly(2,2-dimethoxy nitrobenzyl methacrylate-r-methyl methacrylate-r-poly(ethylene glycol) methacrylate) (PDMP) was synthesized and used. By performing microscope projection photolithography (MPP) to the PDMP thin films within PDMS microchannels, complex micropatterns of proteins were successfully generated within microfluidic channels.
authors.forename: Miju
authors.initials: M
authors.lastname: Kim
authors.forename: Junsang
authors.initials: J
authors.lastname: Doh
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D008163:Luminescent Measurements; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D004129:Dimethylpolysiloxanes; D007211:Indoles; D011108:Polymers; D011506:Proteins; C568283:polydopamine; C013830:baysilon; D016650:Fluorescein-5-isothiocyanate