title: Production of cell-penetrating peptides in Escherichia coli using an intein-mediated system.
abstract: Cell-penetrating peptides are molecules with the ability to cross membranes and enter cells. Attention has been put on these peptides as a tool for drug delivery research, as they are able to serve as delivery vectors for large molecules. Intracellular delivery of bioactive peptides is a very promising research area for clinical applications, since peptides are able to simulate protein regions and thus modulate key intracellular protein-protein interactions. Therefore, evaluation of different strategies for production of these peptides is necessary. In this work, an intein-mediated system was used to evaluate Escherichia coli recombinant production of p53pAnt and PNC27 anticancer cell-penetrating peptides. It was demonstrated that the pTXB1 and the pTYB11 vector systems are suitable for production of this kind of peptides. The production process involves a low-temperature induction process and an efficient on-column intein-mediated cleavage, which allowed an effective peptide recovery using a single chromatographic step.
authors.affiliation: Centre for Biotechnology and Bioengineering (CeBiB), Department of Chemical Engineering and Biotechnology, University of Chile, Beauchef 851, Santiago, Chile, vidrodri@ing.uchile.cl.
authors.forename: Vida
authors.initials: V
authors.lastname: Rodríguez
authors.forename: Jorge
authors.initials: J
authors.lastname: Lascani
authors.forename: Juan A
authors.initials: JA
authors.lastname: Asenjo
authors.forename: Barbara A
authors.initials: BA
authors.lastname: Andrews
mesh_terms: D057846:Cell-Penetrating Peptides / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D047668:Inteins; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D057846:Cell-Penetrating Peptides; D011993:Recombinant Fusion Proteins