title: Electrochemical biosensors based on ferroceneboronic Acid and its derivatives: a review.
abstract: We review recent progress in the development of electrochemical biosensors based on ferroceneboronic acid (FcBA) and ferrocene (Fc)-modified boronic acids. These compounds can be used to construct electrochemical biosensors because they consist of a binding site (i.e., a boronic acid moiety) and an electrochemically active part (i.e., an Fc residue). By taking advantage of the unique properties of FcBA and its derivatives, electrochemical sensors sensitive to sugars, glycated hemoglobin (HbA1c), fluoride (F(-)) ions, and so forth have been widely studied. FcBA-based sugar sensors rely on the selective binding of FcBA to 1,2- or 1,3-diol residues of sugars through the formation of cyclic boronate ester bonds. The redox properties of FcBA-sugar adduct differ from those of free FcBA, which forms the basis of the electrochemical determination of sugars. Thus, non-enzymatic glucose sensors are now being actively studied using FcBA and Fc-modified boronic acids as redox markers. Using a similar principle, HbA1c can be detected by FcBA-based electrochemical systems because it contains hydrocarbon chains on the polypeptide chain. HbA1c sensors are useful for monitoring blood glucose levels over the preceding 8-12 weeks. In addition, FcBA and Fc-modified boronic acids have been used for the detection of F(-) ions due to the selective binding of boronic acid to F(-) ions. F(-)-ion sensors may be useful alternatives to conventional ion-selective electrodes sensitive to F(-) ion. Furthermore, FcBA derivatives have been studied to construct lectin; steroids; nucleotides; salicylic acid; and bacteria sensors. One of the limitations of FcBA-based sensors comes from the fact that FcBA derivatives are added in sample solutions as reagents. FcBA derivatives should be immobilized on the surface of electrodes for developing reagentless sensors.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Shandong University, 44 Wenhua Xilu, Jinan, Shandong 250012, China; E-Mail: bzhenw@hotmail.com ; Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan; E-Mail: t-shigehiro@m.tohoku.ac.jp.
authors.forename: Baozhen
authors.initials: B
authors.lastname: Wang
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan; E-Mail: t-shigehiro@m.tohoku.ac.jp.
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Public Health College, Harbin Medical University, Harbin 150081, China; E-Mail: duxiaoyanha@163.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Du
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Aramaki, Aoba-ku, Sendai 980-8578, Japan; E-Mail: t-shigehiro@m.tohoku.ac.jp.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Anzai
keywords: F−-ion sensor; HbA1c sensor; electrochemical sensor; ferrocene-modified boronic acid; ferroceneboronic acid; glucose sensor