title: Gold nanoparticles conjugated dopamine as sensing platform for SERS detection.
abstract: In this work, the properties of dopamine and dopamine-quinone on gold nanoparticles (Au NPs) surface were studied by the constructed Au NPs/dopamine sensing platform using surface enhanced Raman scattering (SERS) spectroscopy. Interestingly, the Au NPs/dopamine-quinone exhibited the characteristic Raman band at 1270, 1335 and 1480 cm(-1) at pH 10.0, whereas, no obvious Raman band of Au NPs/dopamine was observed at pH = 6.0. Also, dopamine-quinone could be reduced by glutathione (GSH) and dopamine could be oxidized easily by superoxide radical anion (O2(-)), thus, this sensing platform could be used to determine the concentration of GSH and O2(-) in a wide range. Importantly, the utility of Au NPs/dopamine platform was demonstrated in living HeLa and normal human liver (HL-7702) cells, and responded to the concentration changes of reactive oxygen species (ROS) in real time.
authors.affiliation: School of Chemical, Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Chemical, Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China.
authors.forename: Xiangqing
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemical, Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China.
authors.forename: Shi-Zhao
authors.initials: SZ
authors.lastname: Kang
authors.affiliation: School of Chemical, Environmental Engineering, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China. Electronic address: mujin@sit.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Mu
mesh_terms: D002460:Cell Line; D004298:Dopamine / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000032:analysis; D013059:Spectrum Analysis, Raman; D013481:Superoxides / Q000032:analysis; D013499:Surface Properties
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D006046:Gold; D005978:Glutathione; D004298:Dopamine
keywords: Cells; Dopamine; Gold nanoparticles; Surface enhanced Raman scattering