title: Ethylene and pollination decrease transcript abundance of an ethylene receptor gene in Dendrobium petals.
abstract: We studied the expression of a gene encoding an ethylene receptor, called Ethylene Response Sensor 1 (Den-ERS1), in the petals of Dendrobium orchid flowers. Transcripts accumulated during the young floral bud stage and declined by the time the flowers had been open for several days. Pollination or exposure to exogenous ethylene resulted in earlier flower senescence, an increase in ethylene production and a lower Den-ERS1 transcript abundance. Treatment with 1-methylcyclopropene (1-MCP), an inhibitor of the ethylene receptor, decreased ethylene production and resulted in high transcript abundance. The literature indicates two kinds of ethylene receptor genes with regard to the effects of ethylene. One group shows ethylene-induced down-regulated transcription, while the other has ethylene-induced up-regulation. The present gene is an example of the first group. The 5' flanking region showed binding sites for Myb and myb-like, homeodomain, MADS domain, NAC, TCP, bHLH and EIN3-like transcription factors. The binding site for the EIN3-like factor might explain the ethylene effect on transcription. A few other transcription factors (RAV1 and NAC) seem also related to ethylene effects.
authors.affiliation: Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand; Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand. Electronic address: monthatipt@yahoo.com.
authors.forename: Monthathip
authors.initials: M
authors.lastname: Thongkum
authors.affiliation: Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand; Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Parichart
authors.initials: P
authors.lastname: Burns
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Anjana
authors.initials: A
authors.lastname: Bhunchoth
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Nuchnard
authors.initials: N
authors.lastname: Warin
authors.affiliation: National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Thanon Phahonyothin, Tambon Khlong Nueng, Amphoe Khlong Luang, Pathum Thani 12120, Thailand.
authors.forename: Orawan
authors.initials: O
authors.lastname: Chatchawankanphanich
authors.affiliation: Mann Laboratory, Department of Plant Sciences, University of California, Davis, CA 95616, USA.
authors.forename: Wouter G
authors.initials: WG
authors.lastname: van Doorn
mesh_terms: D024506:5' Flanking Region / Q000235:genetics; D003198:Computer Simulation; D003521:Cyclopropanes / Q000494:pharmacology; D031667:Dendrobium / Q000187:drug effects / Q000235:genetics*; D005030:Ethylenes / Q000494:pharmacology*; D035264:Flowers / Q000187:drug effects / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054817:Pollination / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D003521:Cyclopropanes; D005030:Ethylenes; D010940:Plant Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C036216:ethylene; C412563:1-methylcyclopropene
keywords: Dendrobium; Ethylene receptor; Flower senescence; Promoter analysis