title: Recombinant Cpn 0810 stimulates proinflammatory cytokine expression and apoptosis in human monocytes.
abstract: The aim of the present study was to express the recombinantChlamydophila pneumoniae(C. pneumoniae) protein, Cpn 0810, inEscherichia coli(E. coli) BL21, and investigate the effects of Cpn 0810 on inflammatory and apoptotic processes in human monocytic (THP-1) cells. An ELISA was performed to detect the levels of the proinflammatory cytokines, tumor necrosis factor (TNF)-α and interleukin (IL)-6. In addition, Hoechst 33258 staining and annexin V binding analyses were performed to measure the rates of apoptosis. Purified glutathione S-transferase (GST)-Cpn 0810 recombinant proteins were obtained from theE. coliBL21 cells carrying the pGEX6p-2/Cpn 0810 plasmid, and were shown to stimulate the expression of TNF-α and IL-6 in the THP-1 cells in a dose- and time-dependent manner. TNF-α and IL-6 levels peaked at 24 h after GST-Cpn 0810 stimulation. Furthermore, GST-Cpn 0810 significantly promoted the apoptosis of THP-1 cells. In conclusion, recombinant GST-Cpn 0810 was shown to stimulate the expression of TNF-α and IL-6, inhibit proliferation and induce apoptosis in THP-1 cells. Therefore, Cpn 0810 may interact with host cells followingC. pneumoniaeinfection, functioning as an important pathogenic factor.
authors.affiliation: Department of Inspection, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Inspection, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Baiping
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Liangzhuan
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: You
authors.affiliation: Department of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathogenic Biology, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yimou
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Inspection, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Qiugui
authors.initials: Q
authors.lastname: Zhang
keywords: Chlamydophila pneumoniae; Cpn 0810; THP-1 cells; apoptosis; proinflammatory cytokines