title: RNA-Rocket: an RNA-Seq analysis resource for infectious disease research.
abstract: BACKGROUND
  RNA-Seq is a method for profiling transcription using high-throughput sequencing and is an important component of many research projects that wish to study transcript isoforms, condition specific expression and transcriptional structure. The methods, tools and technologies used to perform RNA-Seq analysis continue to change, creating a bioinformatics challenge for researchers who wish to exploit these data. Resources that bring together genomic data, analysis tools, educational material and computational infrastructure can minimize the overhead required of life science researchers.
  
  
  RESULTS
  RNA-Rocket is a free service that provides access to RNA-Seq and ChIP-Seq analysis tools for studying infectious diseases. The site makes available thousands of pre-indexed genomes, their annotations and the ability to stream results to the bioinformatics resources VectorBase, EuPathDB and PATRIC. The site also provides a combination of experimental data and metadata, examples of pre-computed analysis, step-by-step guides and a user interface designed to enable both novice and experienced users of RNA-Seq data.
  
  
  METHODS
  RNA-Rocket is available at rnaseq.pathogenportal.org. Source code for this project can be found at github.com/cidvbi/PathogenPortal.
  
  
  BACKGROUND
  anwarren@vt.edu
  
  
  BACKGROUND
  Supplementary materials are available at Bioinformatics online.
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Andrew S
authors.initials: AS
authors.lastname: Warren
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Aurrecoechea
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Brunk
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Prerak
authors.initials: P
authors.lastname: Desai
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Scott
authors.initials: S
authors.lastname: Emrich
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Gloria I
authors.initials: GI
authors.lastname: Giraldo-Calderón
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Harb
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Deborah
authors.initials: D
authors.lastname: Hix
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lawson
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Dustin
authors.initials: D
authors.lastname: Machi
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Mao
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: McClelland
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Nordberg
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Maulik
authors.initials: M
authors.lastname: Shukla
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Leslie B
authors.initials: LB
authors.lastname: Vosshall
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Alice R
authors.initials: AR
authors.lastname: Wattam
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Will
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Hyun Seung
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA 24060, USA, Center for Tropical & Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA, Penn Center for Bioinformatics and Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA, Department of Computer Science and Engineering, University of Notre Dame, Notre Dame, IN 46556, USA, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46656-0369, USA, University of California, Department of Microbiology and Molecular Genetics, Irvine, California, USA and The Rockefeller University, Howard Hughes Medical Institute, New York, NY 10065, USA.
authors.forename: Bruno
authors.initials: B
authors.lastname: Sobral
mesh_terms: D000818:Animals; D001419:Bacteria / Q000235:genetics; D004199:Disease Vectors; D020869:Gene Expression Profiling / Q000379:methods*; D023281:Genomics; D057166:High-Throughput Screening Assays / Q000379:methods*; D010271:Parasites / Q000235:genetics; D017423:Sequence Analysis, RNA / Q000379:methods*; D012984:Software