title: Yu Ping Feng San, an Ancient Chinese Herbal Decoction, Induces Gene Expression of Anti-viral Proteins and Inhibits Neuraminidase Activity.
abstract: Yu Ping Feng San (YPFS), a Chinese herbal decoction comprised of Astragali Radix (Huangqi), Atractylodis Macrocephalae Rhizoma (Baizhu) and Saposhnikoviae Radix (Fangfeng), has been used clinically for colds and flus; however, the action mechanism of which is not known. Previously, we had demonstrated that YPFS could modulate inflammatory response and phagocytosis in exerting anti-viral and anti-bacterial effects. Here, we further evaluated the bioactivities of YPFS in gene expression regulated by interferon (IFN) signaling and neuraminidase activity of influenza virus A. Application of YPFS onto cultured murine macrophages, the expressions of mRNAs encoding ribonuclease L (RNaseL), myxovirus (influenza virus) resistance 2 (Mx2), protein kinase R (PKR) and IFN-stimulated gene 15 (ISG15) were induced from 2 to 30 folds in dose-dependent manners. In parallel, the transcriptional activity of IFN-stimulated response element (ISRE), an up stream regulator of the above anti-viral proteins, was also triggered by YPFS treatment. Conversely, YPFS was found to suppress the neuraminidase activity of influenza virus A in cultured epithelial cells, thereby preventing the viral release and spreading. Taken together, YPFS exerted anti-bacterial and anti-viral effects in innate immunity.
authors.affiliation: Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, SAR, China; Department of Biology, Hanshan Normal University, Chaozhou, Guangdong, 521041, China.
authors.forename: Crystal Y Q
authors.initials: CY
authors.lastname: Du
authors.forename: Ken Y Z
authors.initials: KY
authors.lastname: Zheng
authors.forename: Cathy Wc
authors.initials: CW
authors.lastname: Bi
authors.forename: Tina T X
authors.initials: TT
authors.lastname: Dong
authors.forename: Huangquan
authors.initials: H
authors.lastname: Lin
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D004285:Dogs; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004722:Endoribonucleases / Q000378:metabolism; D015870:Gene Expression; D053118:Influenza A Virus, H1N1 Subtype; D008264:Macrophages / Q000187:drug effects*; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D064547:Myxovirus Resistance Proteins / Q000378:metabolism; D009439:Neuraminidase / Q000037:antagonists & inhibitors*; D014452:Ubiquitins / Q000378:metabolism; D014764:Viral Proteins / Q000037:antagonists & inhibitors; D019892:eIF-2 Kinase / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D016207:Cytokines; D004365:Drugs, Chinese Herbal; C489550:G1p2 protein, mouse; C579067:Mx2 protein, mouse; D064547:Myxovirus Resistance Proteins; D014452:Ubiquitins; D014764:Viral Proteins; C506362:yu ping feng san; D019892:eIF-2 Kinase; D004722:Endoribonucleases; C031956:2-5A-dependent ribonuclease; D009439:Neuraminidase
keywords: ISG15; Mx2; PKR; RNaseL; Yu Ping Feng San; neuraminidase