title: The Study of Different Approaches of Parecoxib Sodium Pretreatment on the Behavior of Rats with Neuropathic Pain.
abstract: Our objective is to analyze and observe the different administration routes of parecoxib sodium pretreatment on the behavioral improvement of rats with neuropathic pain to provide the preclinical data of parecoxib sodium on neuropathic pain treatment. 30 SD rats were randomly divided into five groups, including model group, sham operation group, intrathecal injection group (IT group), intraperitoneal injection group (IP group), and perineural infiltration group (PI group). The rats in model group and three parecoxib sodium pretreatment groups received spinal nerve ligation (SNL). Heat pain test and 50 % paw mechanical withdrawal threshold test (50 % PMWT) were use to assess the responses after parecoxib sodium pretreatment. 50 % PMWT results of right foot in five groups had no statistical difference (P > 0.05); 50 % PMWT results of left and right feet in three parecoxib sodium pretreatment groups were obviously higher than SNL group at different time points, which was statistically different (P < 0.05); in comparison with three pretreatment groups, the data of left foot in IT group were obviously higher than PI group and IP group, and the comparison among three groups had significant difference (P < 0.05). However, the data of right foot had no significant difference among three groups (P > 0.05). Paw thermal withdrawal latency (PTWL) results of left and right feet in five groups had no significant difference before surgery (P > 0.05); after the establishment of neuropathic model, PTWL results in five groups were significantly decreased; however, PTWL results of left and right feet at 3 days after surgery in IT group were significantly higher than the two other pretreatment groups (P < 0.05); PTWL results of left and right feet at 7 and 14 days after surgery had no significant difference. Parecoxib sodium pretreatment can effectively improve the behaviors caused by neuropathic pain, and intrathecal injection is the most effective route of administration.
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, China Medical University, NO.155 of Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning, China. drwenyaocui@163.com.
authors.forename: Wenyao
authors.initials: W
authors.lastname: Cui
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, China Medical University, NO.155 of Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Anesthesiology, The First Affiliated Hospital, China Medical University, NO.155 of Nanjingbei Street, Heping District, Shenyang, 110001, Liaoning, China.
authors.forename: Huiqian
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000700:Analgesics / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D004334:Drug Administration Schedule; D006358:Hot Temperature; D007249:Inflammation; D007274:Injections, Intraperitoneal; D007278:Injections, Spinal; D007555:Isoxazoles / Q000008:administration & dosage*; D008297:Male; D009437:Neuralgia / Q000188:drug therapy*; D059408:Pain Management; D010147:Pain Measurement; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013127:Spinal Nerves / Q000601:surgery
chemical_list: D000700:Analgesics; D007555:Isoxazoles; C409945:parecoxib
keywords: Different approaches; Neuropathic pain; Parecoxib sodium; Pretreatment; Rat behavior