title: Effects of azithromycin onPseudomonas aeruginosaisolates from catheter-associated urinary tract infection.
abstract: Pseudomonas aeruginosais a common pathogenic bacterium in urinary tract infections (UTIs), particularly catheter-associated UTIs. The aim of this study was to investigate the effect of azithromycin (AZM) onP. aeruginosaisolated from UTIs. Isolates were identified by biochemical assays and the Vitek system. Antimicrobial susceptibility was determined using the disk diffusion assay. Biofilm formation and adhesion were assayed using a crystal violet staining method. The swimming motility was assayed on agar plates. The elastase activity and rhamnolipid production were determined by the elastin-Congo red method and orcinol reaction, respectively. A total of 32 bacterial isolates were collected from 159 urinary catheters and eight of them wereP. aeruginosaisolates. The results showed that theP. aeruginosaisolates had stronger biofilm formation capability and the biofilms were thicker than those ofP. aeruginosaPAO1. AZM inhibited biofilm formation and adhesion on urinary catheters, and also decreased swimming motility and the production of virulence factors. The results of this study indicated that AZM is potentially a good choice for use in the treatment of UTIs.
authors.affiliation: Urologic Institute of Chongqing Red Cross Hospital, Chongqing 400020, P.R. China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Xu
authors.affiliation: Urologic Institute of Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Urologic Institute of Chongqing Red Cross Hospital, Chongqing 400020, P.R. China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: He
authors.affiliation: Urologic Institute of Chongqing Red Cross Hospital, Chongqing 400020, P.R. China.
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Xu
authors.affiliation: Urologic Institute of Chongqing Red Cross Hospital, Chongqing 400020, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Urologic Institute of Chongqing Red Cross Hospital, Chongqing 400020, P.R. China.
authors.forename: Huan-Sheng
authors.initials: HS
authors.lastname: Jin
keywords: Pseudomonas aeruginosa; azithromycin; biofilm; swimming; urinary tract infection; virulence factor