title: A comparative study of smart spectrophotometric methods for simultaneous determination of a skeletal muscle relaxant and an analgesic in combined dosage form.
abstract: Six simple, specific, accurate and precise spectrophotometric methods were developed and validated for the simultaneous determination of the analgesic drug; paracetamol (PARA) and the skeletal muscle relaxant; dantrolene sodium (DANT). Three methods are manipulating ratio spectra namely; ratio difference (RD), ratio subtraction (RS) and mean centering (MC). The other three methods are utilizing the isoabsorptive point either at zero order namely; absorbance ratio (AR) and absorbance subtraction (AS) or at ratio spectrum namely; amplitude modulation (AM). The proposed spectrophotometric procedures do not require any preliminary separation step. The accuracy, precision and linearity ranges of the proposed methods were determined. The selectivity of the developed methods was investigated by analyzing laboratory prepared mixtures of the drugs and their combined dosage form. Standard deviation values are less than 1.5 in the assay of raw materials and capsules. The obtained results were statistically compared with each other and with those of reported spectrophotometric ones. The comparison showed that there is no significant difference between the proposed methods and the reported methods regarding both accuracy and precision.
authors.affiliation: Analytical Chemistry Department, Faculty of Pharmacy, Deraya University, 14511 Cairo, Egypt.
authors.forename: Hesham
authors.initials: H
authors.lastname: Salem
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts, 11787 6 October City, Egypt; Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795 Cairo, Egypt. Electronic address: daliammamdouh@gmail.com.
authors.forename: Dalia
authors.initials: D
authors.lastname: Mohamed
mesh_terms: D000082:Acetaminophen / Q000032:analysis*; D000700:Analgesics / Q000032:analysis*; D003620:Dantrolene / Q000032:analysis*; D004338:Drug Combinations; D057230:Limit of Detection; D009465:Neuromuscular Agents / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
chemical_list: D000700:Analgesics; D004338:Drug Combinations; D009465:Neuromuscular Agents; D000082:Acetaminophen; D003620:Dantrolene
keywords: Absorbance subtraction method; Amplitude modulation method; Dantrelax compound® capsules; Dantrolene sodium; Paracetamol