title: Fndc5 overexpression facilitated neural differentiation of mouse embryonic stem cells.
abstract: Fndc5 has been recently recognized as a myokine which could be cleaved and secreted into blood stream. It is termed as irisin with an important role in thermogenesis and energy homeostasis. Increased expression of Fndc5 has been reported upon retinoic acid treatment during neural differentiation and its knockdown decreased neural differentiation and neurite outgrowth. This study tries to evaluate the effect of Fndc5 overexpression on rate of neural differentiation in mouse. (Thus, transduced cell line of mouse embryonic stem cell with ability to express Fndc5 under Doxycycline treatment was established. Subsequently, the effect of overexpression of Fndc5 on different stages of neural differentiation was studied). Our study showed an increase enhancement in neuronal precursor markers and mature neuron markers upon overexpression of Fndc5, concluding that Fndc5 facilitates neural differentiation. This effect might be related to increased expression of BDNF following overexpression of Fndc5. Our findings are consistent with recent studies reporting a similar role for Fndc5 in proliferation of neural cells and increase in the expression of neurotrophins like BDNF.
authors.affiliation: Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran; Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Forouzanfar
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Rabiee
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.forename: Siamak
authors.initials: S
authors.lastname: Beheshti
authors.forename: Somayeh
authors.initials: S
authors.lastname: Tanhaei
authors.forename: Alireza
authors.initials: A
authors.lastname: Shoaraye Nejati
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jodeiri Farshbaf
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D005353:Fibronectins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism / Q000502:physiology*; D055495:Neurogenesis / Q000235:genetics*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D019208:Brain-Derived Neurotrophic Factor; C571428:FNDC5 protein, mouse; D005353:Fibronectins
keywords: BDNF; gene expression; mouse embryonic stem cell; neural differentiation