title: Long noncoding RNA PEG10 regulates proliferation and invasion of esophageal cancer cells.
abstract: Long chain noncoding RNAs (LncRNAs), a class of noncoding RNA nucleotides longer than 200 bp, have important roles in a variety of biological processes. Accumulating evidence has confirmed the involvement of LncRNAs in cancer initiation, development and progression. We investigated the expression of LncRNA PEG10 in a cohort of esophageal carcinomas to assess its impact on esophageal cancer cell proliferation, apoptosis and invasion. Quantitaive reverse transcription polymerase chain reaction assays were used to quantify LncRNA PEG10 expression levels in 43 paired EC samples and adjacent non-neoplastic tissues. Cell growth, apoptosis and Transwell invasion assays were used to evaluate the effects of LncRNA PEG10 on esophageal cancer cells. LncRNA PEG10 was expressed at higher levels in esophageal cancer tissues than in adjacent non-neoplastic tissues (P<0.05). This relatively high expression was significantly associated with the occurrence of lymph node metastases (P<0.05). Apoptosis and migration rates were significantly decreased in two esophageal cancer cell lines (EC9706 and KYSE150) transfected with si-LncRNA PEG10 (P<0.05). Downregulation of LncRNA PEG10 decreased the expression of PEG10 (P<0.05). Our results indicated that LncRNA PEG10 is upregulated in esophageal cancer tissues, and its downregulation inhibits proliferation and invasion, and promotes apoptosis in esophageal cancer cells. LncRNA PEG10 may serve as a therapeutic agent in esophageal cancer.
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: W
authors.initials: W
authors.lastname: Zang
authors.affiliation: Department of Hemato-tumor, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, People's Republic of China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People's Republic of China.
authors.forename: G
authors.initials: G
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D049109:Cell Proliferation; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009928:Organ Specificity; D062085:RNA, Long Noncoding / Q000502:physiology*
chemical_list: C000603989:PEG10 non-coding RNA, human; D062085:RNA, Long Noncoding