title: Developmental patterns of emission of scent compounds and related gene expression in roses of the cultivar Rosa x hybrida cv. 'Yves Piaget'.
abstract: 2-Phenylethanol (2PE) and 3,5-dimethoxytoluene (DMT) are characteristic scent compounds in specific roses such as Rosa x hybrida cv. 'Yves Piaget'. We analyzed the endogenous concentrations and emission of 2PE and DMT during the unfurling process in different floral organs, as well as changes in transcript levels of the two key genes, PAR and OOMT2. The emission of both 2PE and DMT increased during floral development to reach peaks at the fully unfurled stage. The relative transcripts of PAR and OOMT2 also increased during floral development. Whereas the maximum for OOMT2 was found at the fully unfurled stage (stage 4), similar expression levels of PAR were detected at stage 4 and the senescence stage (stage 6). The results demonstrate a positive correlation between the expression levels of PAR and OOMT2 and the emission of 2PE and DMT. In addition, endogenous volatiles and relative transcripts showed tissue- and development-specific patterns.
authors.affiliation: Research Academy of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China; The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China. Electronic address: xminch@163.com.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Leibniz-Institute of Vegetable and Ornamental Crops, Grossbeeren/Erfurt e.V., Theodor-Echternmeyer-Weg 1, 14979 Großbeeren, Germany; Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany. Electronic address: baldermann@igzev.de.
authors.forename: Susanne
authors.initials: S
authors.lastname: Baldermann
authors.affiliation: The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China. Electronic address: caoshy815@126.com.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, PR China. Electronic address: 46390184@qq.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Lu
authors.affiliation: The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, PR China. Electronic address: wzcaixia1978@163.com.
authors.forename: Caixia
authors.initials: C
authors.lastname: Liu
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan. Electronic address: lxjtpt341@ybb.ne.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hirata
authors.affiliation: Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu 432-8561, Japan. Electronic address: acnwata@ipc.shizuoka.ac.jp.
authors.forename: Naoharu
authors.initials: N
authors.lastname: Watanabe
mesh_terms: D000840:Anisoles / Q000378:metabolism*; D035264:Flowers / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D010626:Phenylethyl Alcohol / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D027826:Rosa / Q000235:genetics / Q000378:metabolism*
chemical_list: D000840:Anisoles; D010940:Plant Proteins; C465201:3,5-dimethoxytoluene; D010626:Phenylethyl Alcohol
keywords: 2-Phenylethanol; 3,5-Dimethoxytoluene; Floral scent compound; GC–MS; Real time qRT-PCR; Relative transcript level; Rosa x hybrida cv. ‘Yves Piaget’