title: Water-soluble extracts from defatted sesame seed flour show antioxidant activity in vitro.
abstract: Defatted white and gold sesame seed flour, recovered as a byproduct after sesame oil extraction, was extracted with 70% ethanol to obtain polar-soluble crude extracts. The in vitro antioxidant activity of the extract was evaluated by DPPH free radical scavenging activity and oxygen radical absorbing capacity (ORAC). The polar-soluble crude extracts of both sesame seed types exhibited good antioxidant capacity, especially by the ORAC method with 34,720 and 21,700 μmol Trolox equivalent/100g of white and gold sesame seed extract, respectively. HPLC, butanol extraction, and UPLC-MS analyses showed that different compounds contributed to the antioxidant activity of the polar-soluble crude extracts. Sesaminol glycosides were identified in the butanol-soluble fractions; whereas, purified water-soluble fraction contained ferulic and vanillic acids. This study shows that hydrophilic antioxidants in the purified water-soluble fraction contributed to the antioxidant activity of white and gold sesame seed polar-soluble crude extracts.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
authors.forename: Sana
authors.initials: S
authors.lastname: Ben Othman
authors.affiliation: Shinsei Co. Ltd., 29 Katabashinmachi, Kitanagoya, Aichi 481-8526, Japan.
authors.forename: Nakako
authors.initials: N
authors.lastname: Katsuno
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kanamaru
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan. Electronic address: yabet@gifu-u.ac.jp.
authors.forename: Tomio
authors.initials: T
authors.lastname: Yabe
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D005433:Flour / Q000032:analysis*; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D031685:Sesamum / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014867:Water
keywords: DPPH radical scavenging; Hydrophilic antioxidants; ORAC; Sesamum indicum L.; Water-soluble extracts