title: Special AT-rich sequence-binding protein-1 participates in the maintenance of breast cancer stem cells through regulation of the Notch signaling pathway and expression of Snail1 and Twist1.
abstract: The stem cell populations in cancerous tissues and cell lines vary widely and are often associated with aggressive cases of breast cancer. Despite research on the topic, the mechanism underlying the regulation of the breast cancer stem cell (BCSC) population within tumors remains to be fully elucidated. To investigate the function of special AT‑rich sequence‑binding protein‑1 (SATB1) in the maintenance of the BCSC population, SATB1 was overexpressed with lentivirus in MCF‑7 cells or knocked down with shRNA‑lentivirus in BT‑549 cells. The effects of SATB1 overexpression or knockdown on mammosphere formation, the size of the of BCSC population, cell invasion and tumorigenesis were investigated. Activation of the Notch signaling pathway and expression of Snail1 and Twist1 were also examined in the cells. Overexpression of SATB1 in MCF‑7 cells was observed to increase mammosphere formation, the size of the BCSC population, cell invasion and tumorigenesis, accompanied by an increase in the activation of Notch signaling and expression levels of Snail1 and Twist1. Conversely, knockdown of SATB1 in BT‑549 cells produced the opposite effects. The results indicated that expression of SATB1 may increase the size of the BCSC population via the activation of the Notch signaling pathway and by increasing expression levels of Snail1 and Twist1.
authors.affiliation: Department of Breast Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Zhengkui
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Clinical Medicine, Medical School of Nanchang University, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Xuesen
authors.initials: X
authors.lastname: Zou
authors.affiliation: Department of Breast Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Guixiang
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Breast Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Zongquan
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Clinical Medicine, Medical School of Nanchang University, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Clinical Medicine, Medical School of Nanchang University, Nanchang, Jiangxi 330029, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xie
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D000066673:Cell Self Renewal; D002471:Cell Transformation, Neoplastic / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D064593:Heterografts; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D016130:Immunophenotyping; D061986:MCF-7 Cells; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics*; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D000071250:Snail Family Transcription Factors; D018874:Spheroids, Cellular; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured; D051879:Twist-Related Protein 1 / Q000235:genetics*
chemical_list: D018960:Hyaluronan Receptors; D036961:Matrix Attachment Region Binding Proteins; D009687:Nuclear Proteins; D051880:Receptors, Notch; C076189:SATB1 protein, human; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors; C103173:TWIST1 protein, human; D014157:Transcription Factors; D051879:Twist-Related Protein 1