title: Effects of resveratrol on the treatment of inflammatory response induced by severe burn.
abstract: The aim of this study was to preliminarily investigate the effects of resveratrol on the treatment of systemic inflammatory response induced by severe burn wounding. Through the simulation experiment in vivo on burned mice and simulative experiment in vitro on mice macrophage respectively, differences of the related pro-inflammatory cytokines and SIRT1 expression levels between the resveratrol-treated group and the untreated control group were detected and analyzed. The results of the simulation experiment in vivo on burned mice manifested that the survival rate of the mice in the resveratrol-treated group was markedly higher than that of controls (p<0.05). Resveratrol could significantly reduce the levels of pro-inflammatory factors TNF-α, IL-1β, and IL-6 in serum (p<0.01) and greatly elevate the expression level of SIRT1 (p<0.01). The results of the simulative experiment in vitro on mice macrophage showed no significant difference in TNF-α, IL-1β, or IL-6 contents among three groups (C, mice macrophage control group; R, resveratrol-treated macrophage group; I, SIRT1-inhibitor-treated macrophage group). Whereas, after lipopolysaccharide (LPS) activation (L group), macrophage TNF-α, IL-1β, and IL-6 levels were significantly increased in L group, dramatically higher than those in L+R group (LPS and resveratrol treatment group) (p<0.01). After adding SITR1 inhibitor, three pro-inflammatory cytokines in L+R+I group all showed significant increases compared with those in L+R group (p<0.01). LPS activated macrophages were able to promote the expression of pro-inflammatory cytokines. By upregulating the expression levels of SIRT1, resveratrol could effectively inhibit the inflammation cascade reaction and increase the survival rate of severe burn with bacterial infections in a large extent.
authors.affiliation: Department of Burn and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, No. 127 West Changle Road, Xi' an, 710032, Shaanxi Province, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Tao
authors.forename: Xiaozhi
authors.initials: X
authors.lastname: Bai
authors.forename: Wenbin
authors.initials: W
authors.lastname: Jia
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiongxiang
authors.initials: X
authors.lastname: Zhu
authors.forename: Juntao
authors.initials: J
authors.lastname: Han
authors.forename: Maolong
authors.initials: M
authors.lastname: Dong
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Dongdong
authors.initials: D
authors.lastname: Chen
authors.forename: Dahai
authors.initials: D
authors.lastname: Hu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D002056:Burns / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D005260:Female; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology; D053583:Interleukin-1beta / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011552:Pseudomonas Infections / Q000276:immunology / Q000401:mortality; D011550:Pseudomonas aeruginosa / Q000276:immunology; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000096:biosynthesis*; D013267:Stilbenes / Q000627:therapeutic use*; D015996:Survival Rate; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C587672:IL1B protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D013267:Stilbenes; D014409:Tumor Necrosis Factor-alpha; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1; D000077185:Resveratrol