title: Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of immobilized enzyme biosensors: Comparison with experiments.
abstract: A description of the implementation of the relaxation method with automatic mesh point allocation for immobilized enzyme electrodes is presented. The advantages of this method for the solution of coupled reaction-diffusion problems are discussed. The relaxation numerical simulation technique is combined with the Simplex fitting algorithm to extract kinetic parameters from experimental data. The results of the simulations are compared to experimental data from self-assembled multilayered electrodes comprised of glucose oxidase (GOx) and an Os modified redox mediator and found to be in excellent agreement.
authors.affiliation: INQUIMAE, Departamento de Quimica Inorganica, Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: V
authors.initials: V
authors.lastname: Flexer
authors.affiliation: City Technology Ltd., Walton Road, Portsmouth, Hants PO6 1SZ, UK.
authors.forename: K F E
authors.initials: KF
authors.lastname: Pratt
authors.affiliation: INFIQC, Departamento de Fisicoquímica, Facultad de Ciencias Químicas, UNC, Pab. Argentina 2° piso, Ciudad Universitaria, Córdoba 5000, Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Garay
authors.affiliation: School of Chemistry, University of Southampton, Southampton, Hants SO17 1BJ, UK.
authors.forename: P N
authors.initials: PN
authors.lastname: Bartlett
authors.affiliation: INQUIMAE, Departamento de Quimica Inorganica, Analitica y Quimica Fisica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: E J
authors.initials: EJ
authors.lastname: Calvo
keywords: Electrostatic self-assembly; Enzyme electrodes; Relaxation method; Simplex algorithm