title: Potential of 5-azacytidine induction decidual stromal cells from maternal human term placenta towards cardiomyocyte-like cells in serum-free medium.
abstract: Decidual stromal cells (DSCs) from maternal term placenta represent a potential source of cells for the treatment of cardiovascular and graft-versus-host diseases. However, it is not clear whether DSCs could be induced towards cardiomyocyte-like differentiation. We chose the placentas which should bred male new-baby. We isolated DSCs from placenta by tissue adherence. The morphology, immunophenotype, and multi-lineage potential were analyzed. Karyotype analysis (G-band) was performed to determine the source and karyotype stability of DSCs. DSCs were induced by 5-azacytidine. Expression of Myf5, α-cardiac actin, Cardiac troponin T (cTnT) and GAPDH was assessed by PCR, and cTnT expression was also analyzed by immunofluorescence. Karyotype analyses indicated that cells were derived from the maternal matrix. After induction with 5-azacytidine, DSCs expressed the cardiac-specific markers Myf5, myogenin and cTnT, indicating differentiation towards cardiomyocyte-like cells.
authors.affiliation: Department of Cardiology, Taizhou Renmin Hospital, 210 Yingchun Road, Taizhou, Jiangsu Province, China.
authors.forename: Gecai
authors.initials: G
authors.lastname: Chen
authors.forename: Aihuan
authors.initials: A
authors.lastname: Yue
authors.forename: Zhongbao
authors.initials: Z
authors.lastname: Ruan
authors.forename: Yigang
authors.initials: Y
authors.lastname: Yin
authors.forename: Ruzhu
authors.initials: R
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Ren
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D001374:Azacitidine / Q000494:pharmacology*; D061249:Batch Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D003656:Decidua / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D004791:Enzyme Inhibitors / Q000008:administration & dosage; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D001374:Azacitidine