title: Enhancing Phytoremediation Potential of Pennisetum clandestinum Hochst in Cadmium-Contaminated Soil Using Smoke-Water and Smoke-Isolated Karrikinolide.
abstract: The use of plant growth regulators (PGRs) and biostimulants to enhance phytoextraction is gaining popularity in phytoremediation technology. This study investigated the stimulatory effects of smoke-water (SW), a smoke-derived compound karrikinolide (KAR1) and other known plant growth regulators (PGRs) [gibberellic acid (GA3), kinetin (Kin) and indole-3-butyric acid (IBA)] to enhance the phytoextraction potential of Pennisetum clandestinum. Pennisetum clandestinum seedlings were grown for 10 weeks in vermiculite using Hoagland's nutrient solution and were treated with cadmium (Cd) (2, 5, and 10 mg L(-1)) and SW, KAR1 and PGRs. KAR1 exhibited positive effects on shoot and root dry weight (140 and 137 mg respectively) at the highest concentration of Cd (10 mg L(-1)) compared to all the other treatments. KAR1 and SW treatments used in the present study significantly improved the phytoextraction potential of P. clandestinum (602 and 575 mg kg(-1) respectively) compared to the other tested PGRs. This is the first report on the use of SW and KAR1 to enhance phytoremediation potential in P. clandestinum. Further studies are needed to elucidate the exact mechanisms of smoke constituents involved in phytoextraction potential of plant species.
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal Pietermaritzburg , Scottsville , South Africa.
authors.forename: Ambrose
authors.initials: A
authors.lastname: Okem
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal Pietermaritzburg , Scottsville , South Africa.
authors.forename: Manoj G
authors.initials: MG
authors.lastname: Kulkarni
authors.affiliation: a Research Centre for Plant Growth and Development, School of Life Sciences , University of KwaZulu-Natal Pietermaritzburg , Scottsville , South Africa.
authors.forename: Johannes
authors.initials: J
authors.lastname: Van Staden
mesh_terms: D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism*; D005663:Furans / Q000494:pharmacology; D031782:Pennisetum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology; D012906:Smoke / Q000032:analysis*; D012989:Soil Pollutants / Q000378:metabolism*; D014867:Water / Q000737:chemistry
chemical_list: D005663:Furans; D010937:Plant Growth Regulators; D011714:Pyrans; D012906:Smoke; D012989:Soil Pollutants; C557869:karrikinolide; D002104:Cadmium; D014867:Water
keywords: Pennisetum clandestinum; heavy metal; phytoextraction; phytoremediation; plant growth regulator; smoke solution