title: Functionalized PCL/HA nanocomposites as microporous membranes for bone regeneration.
abstract: In the present work, microporous membranes based on poly(ε-caprolactone) (PCL) and PCL functionalized with amine (PCL-DMAEA) or anhydride groups (PCL-MAGMA) were realized by solvent-non solvent phase inversion and proposed for use in Guided Tissue Regeneration (GTR). Nanowhiskers of hydroxyapatite (HA) were also incorporated in the polymer matrix to realize nanocomposite membranes. Scanning Electron Microscopy (SEM) showed improved interfacial adhesion with HA for functionalized polymers, and highlighted substantial differences in the porosity. A relationship between the developed porous structure of the membrane and the chemical nature of grafted groups was proposed. Compared to virgin PCL, hydrophilicity increases for functionalized PCL, while the addition of HA influences significantly the hydrophilic characteristics only in the case of virgin polymer. A significant increase of in vitro degradation rate was found for PCL-MAGMA based membranes, and at lower extent of PCL-DMAEA membranes. The novel materials were investigated regarding their potential as support for cell growth in bone repair using multipotent mesenchymal stromal cells (MSC) as a model. MSC plated onto the various membranes were analyzed in terms of adhesion, proliferation and osteogenic capacity that resulted to be related to chemical as well as porous structure. In particular, PCL-DMAEA and the relative nanocomposite membranes are the most promising in terms of cell-biomaterial interactions.
authors.affiliation: Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples), Italy.
authors.forename: Maria Assunta
authors.initials: MA
authors.lastname: Basile
authors.affiliation: Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples), Italy.
authors.forename: Giovanna Gomez
authors.initials: GG
authors.lastname: d'Ayala
authors.affiliation: Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples), Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Malinconico
authors.affiliation: Institute for Polymers, Composites and Biomaterials, CNR, Via Campi Flegrei 34, Pozzuoli (Naples), Italy. Electronic address: paola.laurienzo@ipcb.cnr.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Laurienzo
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, CNRS UMR 5247, University of Montpellier 1, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Coudane
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, CNRS UMR 5247, University of Montpellier 1, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Nottelet
authors.affiliation: Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples, Italy.
authors.forename: Fulvio Della
authors.initials: FD
authors.lastname: Ragione
authors.affiliation: Department of Biochemistry and Biophysics, Second University of Naples, Via L. De Crecchio 7, Naples, Italy. Electronic address: adriana.oliva@unina2.it.
authors.forename: Adriana
authors.initials: A
authors.lastname: Oliva
mesh_terms: D001861:Bone Regeneration; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite
keywords: Biocompatibility; Functionalization of polymers; Guided tissue regeneration; Membranes; Nanocomposites; Poly(ε-caprolactone)