title: Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.
abstract: The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.
authors.affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Resources and Environment, Southwest University, Chongqing, 400716, P.R. China.
authors.forename: Shui-Ping
authors.initials: SP
authors.lastname: Yang
authors.affiliation: Chongqing Academy of Chinese Materia Medica, Chongqing 400065, P.R.China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China.
authors.forename: Qi-Yi
authors.initials: QY
authors.lastname: He
authors.affiliation: College of Bioengineering, Chongqing University, Chongqing 400044, PR China.
authors.forename: Bo-Chu
authors.initials: BC
authors.lastname: Wang
keywords: Common peaks number; HPLC fingerprint; The multiple indicator; The percentage of non-common peaks and similarity.; Wuwei Changyanning granule