title: Aneuploidy in Lewy body diseases.
abstract: An increase in DNA content is associated with neuronal degeneration in Alzheimer's disease but has not been evaluated in Lewy body diseases. Using stereological principles, flow cytometry, and standard histopathologic methods, we evaluated the number and DNA content of neurons and all cells and the severity of Lewy and Alzheimer pathologies, in brain regions affected at different stages in Lewy body diseases compared with controls. An increase in neuronal DNA content was observed in all the affected brain regions examined, although this change was related to different pathologies. In the substantia nigra, increased neuronal DNA content related to neuronal loss, whereas in the cortex and hippocampus, increased neuronal DNA content related to Alzheimer pathologies. Of note, increased neuronal DNA content did not relate to the deposition of Lewy bodies in any region examined. These data support the concept that increased DNA content increases neuronal susceptibility to degeneration and Alzheimer pathologies.
authors.affiliation: Neuroscience Research Australia, Randwick, New South Wales, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Neuroscience Research Australia, Randwick, New South Wales, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia.
authors.forename: Claire
authors.initials: C
authors.lastname: Shepherd
authors.affiliation: Neuroscience Research Australia, Randwick, New South Wales, Australia; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. Electronic address: g.halliday@neura.edu.au.
authors.forename: Glenda
authors.initials: G
authors.lastname: Halliday
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D004247:DNA / Q000378:metabolism; D005260:Female; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016631:Lewy Bodies / Q000378:metabolism / Q000473:pathology; D020961:Lewy Body Disease / Q000235:genetics* / Q000473:pathology*; D008297:Male; D009410:Nerve Degeneration / Q000473:pathology; D016874:Neurofibrillary Tangles / Q000473:pathology; D051844:alpha-Synuclein / Q000378:metabolism
chemical_list: D051844:alpha-Synuclein; D004247:DNA
keywords: Aneuploidy; Lewy bodies; Lewy body diseases; Neurofibrillary tangles; Neuronal degeneration; β-amyloid plaques