title: Determination of Neurotoxic Acetogenins in Pawpaw (Asimina triloba) Fruit by LC-HRMS.
abstract: The concentrations of the neurotoxins, annonacin and squamocin, were determined in a lyophilized sample of the fruit pulp of the North American pawpaw (Asimina triloba) by LC coupled to high resolution mass spectrometry or LC-HRMS. The sample was extracted using dry methanol at 100 °C and 10 MPa pressure in a sealed container. The extraction of annonacin and squamocin was optimal at 100 °C with 7.72 and 0.162 mg/g, respectively, being found. Also, several isomers of annonacin and squamocin were separated and detected but not quantified.
authors.affiliation: U.S. Food and Drug Administration, Total Diet and Pesticide Research Center, 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Levine
authors.affiliation: U.S. Food and Drug Administration, Total Diet and Pesticide Research Center, 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Kristy M
authors.initials: KM
authors.lastname: Richards
authors.affiliation: U.S. Food and Drug Administration, Total Diet and Pesticide Research Center, 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Kevin
authors.initials: K
authors.lastname: Tran
authors.affiliation: University of Missouri - St. Louis , One University Drive, St. Louis, Missouri 63121, United States.
authors.forename: Rensheng
authors.initials: R
authors.lastname: Luo
authors.affiliation: Southwest Research Center, University of Missouri , Mt. Vernon, Missouri 65712, United States.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Thomas
authors.affiliation: U.S. Food and Drug Administration, Total Diet and Pesticide Research Center, 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Smith
keywords: Asimina triloba; LC-HRMS; Parkinson’s disease; annonacin; pawpaw; squamocin