title: Bacterial (BKH1) assisted silica nanoparticles from silica rich substrates: a facile and green approach for biotechnological applications.
abstract: A green technique of silica nanoparticles (SiO2-NPs) formation by using a thermophilic bacterium (BKH1) as biological template is demonstrated here. SiO2-NPs are synthesized from inorganic (magnesium tri-silicate), and organic (tetraethyl orthosilicate) precursor with the help of BKH1 bacteria. BKH1 derived SiO2-NPs are subjected to Atomic Force Microscopy, Transmission Electron Microscopy, and Field Emission Scanning Electron Microscopy equipped with Energy Dispersive X-ray Analyzer to establish nanoparticle morphology. In addition, Infrared Spectroscopy reveals the presence of chemical and functional groups in SiO2-NPs samples and X-ray diffraction, the amorphous nature. The Zeta potential (ζ) reveals substantial stability of bacteria derived SiO2-NPs in the aqueous environment. Presence of two intense luminescence peaks in the UV and visible regions merits the bacteria derived SiO2-NPs for use as an optical probe in biomedical applications. This novel mode of bacteria derived SiO2-NPs formation is eco-friendly and ambient temperature synthesis approach. It avoids the complex protocol of multi-steps synthesis of silica nanoparticles, hence likely to be cost-effective. In-depth translation research is suggested for the synthesis of silica nanoparticles in large quantities using thermophilic BKH1 template.
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700032, India.
authors.forename: Shilpi
authors.initials: S
authors.lastname: Show
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700032, India.
authors.forename: Abiral
authors.initials: A
authors.lastname: Tamang
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700032, India.
authors.forename: Trinath
authors.initials: T
authors.lastname: Chowdhury
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700032, India. Electronic address: dipankar@phys.jdvu.ac.in.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Mandal
authors.affiliation: Department of Physics, Jadavpur University, Kolkata 700032, India. Electronic address: bdc_physics@yahoo.co.in.
authors.forename: Brajadulal
authors.initials: B
authors.lastname: Chattopadhyay
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001709:Biotechnology; D055772:Green Chemistry Technology; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D012822:Silicon Dioxide
keywords: Bacterial template; Green chemistry; Magnesium trisilicate; Photoluminance; Silica nanoparticles; Tetraethyl orthosilicate