title: Chromatin looping and eRNA transcription precede the transcriptional activation of gene in the β-globin locus.
abstract: Enhancers are closely positioned with actively transcribed target genes by chromatin looping. Non-coding RNAs are often transcribed on active enhancers, referred to as eRNAs (enhancer RNAs). To explore the kinetics of enhancer-promoter looping and eRNA transcription during transcriptional activation, we induced the β-globin locus by chemical treatment and analysed cross-linking frequency between the β-globin gene and locus control region (LCR) and the amount of eRNAs transcribed on the LCR in a time course manner. The cross-linking frequency was increased after chemical induction but before the transcriptional activation of gene in the β-globin locus. Transcription of eRNAs was increased in concomitant with the increase in cross-linking frequency. These results show that chromatin looping and eRNA transcription precedes the transcriptional activation of gene. Concomitant occurrence of the two events suggests functional relationship between them.
authors.affiliation: *Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
authors.forename: Yea Woon
authors.initials: YW
authors.lastname: Kim
authors.affiliation: *Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
authors.forename: Sungkung
authors.initials: S
authors.lastname: Lee
authors.affiliation: *Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
authors.forename: Jangmi
authors.initials: J
authors.lastname: Yun
authors.affiliation: *Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
authors.forename: AeRi
authors.initials: A
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D042002:Chromatin Assembly and Disassembly / Q000502:physiology*; D056426:Genetic Loci / Q000502:physiology*; D051379:Mice; D012313:RNA / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation / Q000502:physiology*; D055544:beta-Globins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D002843:Chromatin; D055544:beta-Globins; D012313:RNA