title: Effects of microwaves (900 MHz) on peroxidase systems: A comparison between lactoperoxidase and horseradish peroxidase.
abstract: This work shows the effects of exposure to an electromagnetic field at 900 MHz on the catalytic activity of the enzymes lactoperoxidase (LPO) and horseradish peroxidase (HRP). Experimental evidence that irradiation causes conformational changes of the active sites and influences the formation and stability of the intermediate free radicals is documented by measurements of enzyme kinetics, circular dichroism spectroscopy (CD) and cyclic voltammetry.
authors.affiliation: a Department of Chemistry , "Sapienza" University of Rome , Rome , Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Barteri
authors.affiliation: b National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA) , Rome , Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: De Carolis
authors.affiliation: c National Research Council of Italy (CNR), Instituto di Genetica Molecolare (IGM) , Bologna , Italy.|d SC Laboratory of Muscoloskeletal Cell Biology, Istituto Ortopedico Rizzoli (IOR) , Bologna , Italy.
authors.forename: Fiorenzo
authors.initials: F
authors.lastname: Marinelli
authors.affiliation: e Institute of Radioastronomy, Italian National Institute for Astrophysics (INAF) , Bologna , Italy.
authors.forename: Goliardo
authors.initials: G
authors.lastname: Tomassetti
authors.affiliation: f Department of Biochemistry , "Sapienza" University of Rome , Rome , Italy.
authors.forename: Linda Celeste
authors.initials: LC
authors.lastname: Montemiglio
mesh_terms: D020134:Catalytic Domain / Q000528:radiation effects; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D007784:Lactoperoxidase / Q000737:chemistry / Q000378:metabolism*; D008872:Microwaves / Q000009:adverse effects*
chemical_list: D006735:Horseradish Peroxidase; D007784:Lactoperoxidase
keywords: 900 MHz; electromagnetic fields; enzyme kinetics; horseradish peroxidase; lactoperoxidase; microwaves; radiofrequency