title: The assessment of the relationship between variations in the apelin gene and coronary artery disease in Turkish population.
abstract: OBJECTIVE
  Apelin is a novel endogenous peptide with inotropic and vasodilatory properties and is the ligand for the angiotensin receptor-like 1 (APJ) receptor. The aim of the study was to investigate the association of 2 single-nucleotide polymorphisms (SNPs) in the apelin gene with susceptibility to coronary artery disease (CAD) in the Turkish population.
  
  
  METHODS
  The present observational case-control study consisted of 244 subjects (134 angiographically proven CAD patients and 110 healthy controls) aged 30-65 years. The association of 2 SNPs (rs3115758 and rs3115759) in the apelin gene and CAD risk was investigated. Real-time polymerase chain reaction (RT-PCR) was used to analyze the 2 SNPs in both the CAD and the healthy subjects. Allele and genotype frequencies between patients and control groups were compared using the Chi-square (χ2) test. The relationships of the 2 polymorphisms with the presence of CAD were determined with multiple binary logistic regression analysis after adjustment for CAD risk factors.
  
  
  RESULTS
  TT and AA risk genotypes of the rs3115758 and rs3115759 variants in the apelin gene were found to be significantly related with the risk of CAD with the same power (OR: 6.36, 95% CI: 1.41-28.6) (p=0.007). After adjustments for traditional CAD risk factors, the homozygous TT genotype for rs3115758 and AA genotype for rs3115759 increased the CAD risk, both with an OR of 5.91.
  
  
  CONCLUSIONS
  Genetic variants in the apelin gene are significantly associated with the risk of CAD in the Turkish population.
authors.affiliation: Department of Cardiology, Cerrahpaşa Medical Faculty, İstanbul University; İstanbul-Turkey. ecoskunpinar@gmail.com.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Pakizeh
authors.forename: Ender
authors.initials: E
authors.lastname: Çoşkunpınar
authors.forename: Yasemin Müşteri
authors.initials: YM
authors.lastname: Oltulu
authors.forename: Hüseyin Altuğ
authors.initials: HA
authors.lastname: Çakmak
authors.forename: Barış
authors.initials: B
authors.lastname: İkitimur
authors.forename: Zümrüt Mine
authors.initials: ZM
authors.lastname: Işık Sağlam
authors.forename: Ayla
authors.initials: A
authors.lastname: Karimova
authors.forename: Vural Ali
authors.initials: VA
authors.lastname: Vural
mesh_terms: D000328:Adult; D000368:Aged; D000075244:Apelin Receptors; D016022:Case-Control Studies; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000401:mortality; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D043562:Receptors, G-Protein-Coupled / Q000235:genetics*; D014421:Turkey; D044465:White People / Q000235:genetics
chemical_list: C104575:APLNR protein, human; D000075244:Apelin Receptors; D043562:Receptors, G-Protein-Coupled