title: Detection of blaCTX-M-type genes in complex class 1 integrons carried by Enterobacteriaceae isolated from retail chicken meat in Brazil.
abstract: CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted. In this study, we report for the first time the isolation of multidrug-resistant (MDR) Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis strains harboring blaCTXM-2 or blaCTXM-8 gene variants in chicken meat sold in markets in southeast Brazil. In this regard, the genetic environment of the blaCTX-M-2 gene is composed of a complex class 1 integron and an ISCR1-associated sequence with dfr and/or aadA gene cassettes located within the variable region. In summary, chicken meat may be a reservoir of MDR Enterobacteriaceae harboring blaCTX-M-type genes, which is a public health concern.
authors.affiliation: Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho", São José do Rio Preto, SP, Brazil.
authors.forename: Tiago
authors.initials: T
authors.lastname: Casella
authors.affiliation: Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Ciudad Autónoma de Buenos Aires, BA, Argentina.
authors.forename: María Margarita
authors.initials: MM
authors.lastname: Rodríguez
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
authors.forename: Juliana Tiemi
authors.initials: JT
authors.lastname: Takahashi
authors.affiliation: Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Ciudad Autónoma de Buenos Aires, BA, Argentina.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ghiglione
authors.affiliation: Departamento de Prática de Saúde Pública, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Milena
authors.initials: M
authors.lastname: Dropa
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Ednei
authors.initials: E
authors.lastname: Assunção
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil.
authors.forename: Maurício Lacerda
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil; Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Nilton
authors.initials: N
authors.lastname: Lincopan
authors.affiliation: Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Ciudad Autónoma de Buenos Aires, BA, Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutkind
authors.affiliation: Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brazil. Electronic address: ml.nogueira@famerp.br.
authors.forename: Mara Corrêa Lelles
authors.initials: MC
authors.lastname: Nogueira
mesh_terms: D000818:Animals; D001938:Brazil; D002645:Chickens; D018432:Drug Resistance, Multiple / Q000235:genetics; D004755:Enterobacteriaceae / Q000235:genetics* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D032023:Integrons / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D008460:Meat / Q000382:microbiology*; D011513:Proteus mirabilis / Q000235:genetics / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: CTX-M; Chicken meat; ISCR1; Poultry