title: Assessment of two different types of sample for the early detection and isolation of thermophilic Campylobacter in broiler farms.
abstract: In order to assess the optimal method for the early detection and isolation of thermophilic Campylobacter in broilers at farm level, two types of samples were compared: caecal contents obtained by necropsy and cloacal swabs transported in charcoal Amies medium. The study was conducted in five batches of broilers from five different farms, where weekly samples (caecal contents and cloacal swabs) from 30 birds were obtained. Samples were plated onto selective agar (modified charcoal cefoperazone desoxycholate agar, mCCDA) for Campylobacter isolation. Four out of five batches were positive for Campylobacter. No marked differences in sensitivity of both sample types were observed. However, a higher percentage of positive birds were detected when cloacal swabs were used. The results show that cloacal swab samples are adequate, and in some cases even better than caecal samples for the early detection of Campylobacter in broiler flocks at farm level. Also, this sample avoids sacrificing birds to test Campylobacter, which not only allows saving time in sample collection, transportation and processing at the laboratory, but also improves bird welfare and cost of sampling.
authors.affiliation: a Centre de Recerca en Sanitat Animal (CReSA) , UAB-IRTA, Campus de la Universitat Autònoma de Barcelona , 08193-Bellaterra (Cerdanyola del Vallès) , Spain.
authors.forename: Saulo
authors.initials: S
authors.lastname: Urdaneta
authors.forename: Roser
authors.initials: R
authors.lastname: Dolz
authors.forename: Marta
authors.initials: M
authors.lastname: Cerdà-Cuéllar
mesh_terms: D000818:Animals; D002167:Campylobacter / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000662:veterinary*; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D002988:Cloaca / Q000382:microbiology*; D046508:Culture Techniques / Q000662:veterinary; D017931:DNA Primers / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011201:Poultry Diseases / Q000175:diagnosis* / Q000382:microbiology*; D013048:Specimen Handling / Q000379:methods / Q000662:veterinary*
chemical_list: D017931:DNA Primers