title: Anti-tumor properties of cis-resveratrol methylated analogs in metastatic mouse melanoma cells.
abstract: Resveratrol (E-3,5,4'-trihydroxystilbene) is a polyphenol found in red wine that has been shown to have multiple anti-cancer properties. Although cis-(Z)- and trans-(E)-isomers of resveratrol occur in nature, the cis form is not biologically active. However, methylation at key positions of the cis form results in more potent anti-cancer properties. This study determined that synthetic cis-polymethoxystilbenes (methylated analogs of cis-resveratrol) inhibited cancer-related phenotypes of metastatic B16 F10 and non-metastatic B16 F1 mouse melanoma cells. In contrast with cis- or trans-resveratrol and trans-polymethoxystilbene which were ineffective at 10 μM, cis-polymethoxystilbenes inhibited motility and proliferation of melanoma cells with low micromolar specificity (IC50 < 10 μM). Inhibitory effects by cis-polymethoxystilbenes were significantly stronger with B16 F10 cells and were accompanied by decreased expression of β-tubulin and pleckstrin homology domain-interacting protein, a marker of metastatic B16 cells. Thus, cis-polymethoxystilbenes have potential as chemotherapeutic agents for metastatic melanoma.
authors.affiliation: Department of Chemistry and Biochemistry, Queens College of the City University of New York, Flushing, NY, USA.
authors.forename: Valery L
authors.initials: VL
authors.lastname: Morris
authors.forename: Tayyaba
authors.initials: T
authors.lastname: Toseef
authors.forename: Fathima B
authors.initials: FB
authors.lastname: Nazumudeen
authors.forename: Christian
authors.initials: C
authors.lastname: Rivoira
authors.forename: Carmela
authors.initials: C
authors.lastname: Spatafora
authors.forename: Corrado
authors.initials: C
authors.lastname: Tringali
authors.forename: Susan A
authors.initials: SA
authors.lastname: Rotenberg
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D020128:Inhibitory Concentration 50; D008546:Melanoma, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D013267:Stilbenes; D000077185:Resveratrol