title: An artificial blood vessel implanted three-dimensional microsystem for modeling transvascular migration of tumor cells.
abstract: Reproducing a tumor microenvironment consisting of blood vessels and tumor cells for modeling tumor invasion in vitro is particularly challenging. Here, we report an artificial blood vessel implanted 3D microfluidic system for reproducing transvascular migration of tumor cells. The transparent, porous and elastic artificial blood vessels are obtained by constructing polysaccharide cellulose-based microtubes using a chitosan sacrificial template, and possess excellent cytocompatibility, permeability, and mechanical characteristics. The artificial blood vessels are then fully implanted into the collagen matrix to reconstruct the 3D microsystem for modeling transvascular migration of tumor cells. Well-defined simulated vascular lumens were obtained by proliferation of the human umbilical vein endothelial cells (HUVECs) lining the artificial blood vessels, which enables us to reproduce structures and functions of blood vessels and replicate various hemodynamic parameters. Based on this model, the adhesion and transvascular migration of tumor cells across the artificial blood vessel have been well reproduced.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China. whhuang@whu.edu.cn.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Pei
authors.forename: Min
authors.initials: M
authors.lastname: Xie
authors.forename: Zi-He
authors.initials: ZH
authors.lastname: Jin
authors.forename: Ya-Shi
authors.initials: YS
authors.lastname: Xiao
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Wei-Hua
authors.initials: WH
authors.lastname: Huang
mesh_terms: D001187:Artificial Organs; D001808:Blood Vessels / Q000166:cytology*; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D002482:Cellulose / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008954:Models, Biological; D009369:Neoplasms / Q000473:pathology*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties; D059016:Tumor Microenvironment
chemical_list: D002482:Cellulose; D048271:Chitosan