title: Single-phase dielectrophoretic and electrorotation studies using three dimensional electrodes for cell characterization.
abstract: A novel electrokinetic approach using single-phase electrorotation for the label-free manipulation and characterization of biological cells is presented. A single shell model was used to theoretically design and develop an experimental strategy for biological particle characterization. As a study model, electro-rotation of glutathione agarose (GA) beads was studied using three-dimensional spatially oriented micro-needle setup. Effect of electrical parameters (i) voltage: from 0-10 Vpp and (ii) frequency: 0-100 MHz was evaluated on a heterogeneous mixture of GA beads (35-150 microns). The relationship of the electrical parameters to rotational frequency of the beads was studied. This paper demonstrates a simple and easy to implement prototype for electrokinetic characterization of particles with translational potential for biological cells.
authors.forename: Rajeshwari Taruvai Kalyana
authors.initials: RT
authors.lastname: Kumar
authors.forename: Kavya
authors.initials: K
authors.lastname: Cherukuri
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D004553:Electric Conductivity; D004566:Electrodes; D004586:Electrophoresis / Q000379:methods*; D004594:Electrophysiology; D005978:Glutathione / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000737:chemistry; D005982:Glutathione Transferase / Q000737:chemistry; D007700:Kinetics; D007791:Lactoylglutathione Lyase / Q000737:chemistry; D008853:Microscopy; D008962:Models, Theoretical; D012399:Rotation; D012685:Sepharose / Q000737:chemistry; D019415:Torque
chemical_list: D001672:Biocompatible Materials; D012685:Sepharose; D005979:Glutathione Peroxidase; D005982:Glutathione Transferase; D007791:Lactoylglutathione Lyase; D005978:Glutathione