title: Antibody activation using DNA-based logic gates.
abstract: Oligonucleotide-based molecular circuits offer the exciting possibility to introduce autonomous signal processing in biomedicine, synthetic biology, and molecular diagnostics. Here we introduce bivalent peptide-DNA conjugates as generic, noncovalent, and easily applicable molecular locks that allow the control of antibody activity using toehold-mediated strand displacement reactions. Employing yeast as a cellular model system, reversible control of antibody targeting is demonstrated with low nM concentrations of peptide-DNA locks and oligonucleotide displacer strands. Introduction of two different toehold strands on the peptide-DNA lock allowed signal integration of two different inputs, yielding logic OR- and AND-gates. The range of molecular inputs could be further extended to protein-based triggers by using protein-binding aptamers.
authors.affiliation: Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, De Rondom 70, 5612 AP Eindhoven (The Netherlands).
authors.forename: Brian M G
authors.initials: BM
authors.lastname: Janssen
authors.forename: Martijn
authors.initials: M
authors.lastname: van Rosmalen
authors.forename: Lotte
authors.initials: L
authors.lastname: van Beek
authors.forename: Maarten
authors.initials: M
authors.lastname: Merkx
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D052157:Aptamers, Nucleotide / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D006388:Hemagglutinins / Q000737:chemistry; D007700:Kinetics; D009841:Oligonucleotides / Q000737:chemistry; D009975:Orthomyxoviridae / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000276:immunology
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D006388:Hemagglutinins; D009841:Oligonucleotides; D010455:Peptides; D004247:DNA
keywords: DNA nanotechnology; antibodies; aptamers; molecular computing; peptide-oligonucleotide conjugates