title: The diagnostic value of a new fecal marker, matrix metalloprotease-9, in different types of inflammatory bowel diseases.
abstract: BACKGROUND
  Only limited data are available regarding the diagnostic accuracy of fecal matrix metalloprotease-9 [MMP-9] for inflammatory bowel disease [IBD]. The aims of our study were to assess the diagnostic accuracy of fecal MMP-9 in patients with active Crohn's disease [CD], ulcerative colitis [UC], and pouchitis, and to compare the diagnostic accuracy of fecal MMP-9 and fecal calprotectin [CP] in IBD.
  
  
  METHODS
  Stool and blood samples were collected in 50 CD, 54 UC, and 34 ileal pouch-anal anastomosis patients before control endoscopies were performed. Biopsies were taken for histologic purposes. The activities of CD, UC, and pouchitis were defined with the use of clinical, endoscopic, and histologic activity scores. Fecal CP and MMP-9 levels were quantified by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Active CD, UC, and pouchitis were detected in 38%, 54%, and 29% of the patients, respectively. A significant correlation was revealed between fecal CP and the clinical activities of CD and UC, and between fecal CP and the endoscopic activity of UC and pouchitis. Fecal MMP-9 did not correlate with any of the activity indices of CD; however, strong associations were shown between fecal MMP-9 and clinical, endoscopic, and histologic activities of both UC and pouchitis.
  
  
  CONCLUSIONS
  This is the first study assessing the diagnostic accuracy of MMP-9 in different types of IBD. Our results showed that fecal MMP-9 has high sensitivity in the detection of endoscopically active UC and pouchitis. These non-invasive methods help assess intestinal inflammation.
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Farkas
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Saródi
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Bálint
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Imre
authors.initials: I
authors.lastname: Földesi
authors.affiliation: Department of Pathology, University of Szeged, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Tiszlavicz
authors.affiliation: Department of Medical Physics and Informatics, University of Szeged, Hungary.
authors.forename: Mónika
authors.initials: M
authors.lastname: Szűcs
authors.affiliation: Department of Medical Physics and Informatics, University of Szeged, Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Nyári
authors.affiliation: Department of Surgery, University of Szeged, Hungary.
authors.forename: János
authors.initials: J
authors.lastname: Tajti
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Nagy
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Szepes
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Renáta
authors.initials: R
authors.lastname: Bor
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Annaházi
authors.affiliation: First Department of Medicine, University of Szeged, Hungary.
authors.forename: Richárd
authors.initials: R
authors.lastname: Róka
authors.affiliation: First Department of Medicine, University of Szeged, Hungary molnar.tamas@med.u-szeged.hu.
authors.forename: Tamás
authors.initials: T
authors.lastname: Molnár
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D003113:Colonoscopy; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry*; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis* / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex / Q000378:metabolism; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011446:Prospective Studies; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D039841:Leukocyte L1 Antigen Complex; D020780:Matrix Metalloproteinase 9
keywords: Crohn’s disease; calprotectin; matrix metalloprotease-9; pouchitis; ulcerative colitis