title: Sequencing analysis and characterization of the plasmid pBIF10 isolated from Bifidobacterium longum.
abstract: A resident plasmid, pBIF10, was isolated from Bifidobacterium longum B200304, and the full-length sequence of pBIF10 was analyzed. In this sequence, we identified at least 17 major open reading frames longer than 200 bp. A tetracycline resistance gene, tetQ, was identified and verified to confer antibiotic resistance to tetracycline. The plasmid replicon with replication protein B gene (repB) and a typical iteron was identified in pBIF10. An artificial clone vector was constructed with the replicon of pBIF10; the results showed that repB controlled plasmid replication in other bifidobacteria host cells at low transformation frequency. Taken together, the analysis and characterization of pBIF10 provided necessary information for the understanding of antibiotic resistance mediated by a plasmid in a Bifidobacterium strain. GC% and repB sequence analyses indicated that pBIF10 was a molecular hybrid of at least 2 other bacterial genera plasmids.
authors.affiliation: Key Laboratory of Biochemistry and Molecular Biology, Molecular Medical and Cancer Research Center, Chongqing Medical University, Yuzhong District, Yi XueYuan Road, No. 1, Chongqing 400016, People's Republic of China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Ma
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Xie
authors.forename: Qiongwen
authors.initials: Q
authors.lastname: Hu
authors.forename: Zongyin
authors.initials: Z
authors.lastname: Qiu
authors.forename: Fangzhou
authors.initials: F
authors.lastname: Song
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D001644:Bifidobacterium / Q000235:genetics*; D005243:Feces; D022761:Gene Transfer, Horizontal; D005822:Genetic Vectors; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013753:Tetracycline Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C048817:RepB protein, Bacteria
keywords: Bifidobacterium longum; plasmid; plasmide; replicon