title: [Determination of quercetin and kaempferol in Fagopyrum spp. of different species and different parts by HPLC].
abstract: OBJECTIVE
  An HPLC method was established for determination of quercetin and kaempferol in Fagopyrum spp. of differrent species and different plant parts.
  
  
  METHODS
  The chromatographic separation was carried out on a DIKMA diamonsil (250 mm x 4.6 mm, 5 μm) chromatographic column. The mobile phase was acetonitrile (A) and 0.1% phosphoric acid solution (B) at the flow rate of 1.0 mL/min. The detection wavelength was set at 260 nm and the column temperature was 25 degrees C.
  
  
  RESULTS
  The descending order of quercetin content in the samples of different parts of Fagopyrum tataricum was seed (6.12 mg/g) > leaf (1.47 mg/g) > stem (0.34 mg/g); quercetin was not found in root. Only a few kaempferol was found in seed (0.42 mg/g) and leaf of Xiqiao 1 (0.09 mg/g) of Fagopyrum tataricum. There were a few quercetin was determined in the whole plant of Fagopyrum esculentum, and kaempferol was not been found. The total and mean value concentration of quercetin and kaempferol of Fagopyrum tataricum were much higher than that of Fagopyrum esculentum. In different species of Fagopyrum tataricum planted in the same area, the content of quercetin and kaempferol was similar, they were not been found in Fagopyrum esculentum.
  
  
  CONCLUSIONS
  This method is simple, rapid, accurate and reliable for quality evaluation of Fagopyrum spp. and its commercial products.
authors.affiliation: Sichuan College of Traditional Chinese Medicine, Mianyang, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xia
authors.forename: Yan-fei
authors.initials: YF
authors.lastname: Huang
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Zheng-ming
authors.initials: ZM
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019612:Fagopyrum / Q000737:chemistry*; D044949:Kaempferols / Q000032:analysis*; D018515:Plant Leaves; D018517:Plant Roots; D018547:Plant Stems; D011794:Quercetin / Q000032:analysis*; D012639:Seeds
chemical_list: D044949:Kaempferols; C006552:kaempferol; D011794:Quercetin