title: Manual and rotary instrumentation ability to reduce Enterococcus faecalis associated with photodynamic therapy in deciduous molars.
abstract: This aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (PDT) to reduce Enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue O/laser, fuchsin/halogen light and fuchsin/LED. Twenty deciduous molars were selected and contaminated with Enterococcus faecalis (McFarland 0.5 scale). Working length determination was performed by visual method. The teeth were randomly divided into two groups: G1 (n=10): manual instrumentation (Kerr-type files) and G2 (n=10): rotary instrumentation (ProTaper system). The bacteria were collected three times using sterile paper cones compatible with the anatomic diameter of the root canal for 30 s before and after instrumentation and after PDT. The samples were diluted in peptone water, seeded on blood agar plates and incubated in an oven at 37 °C for colony-forming units counting. The decrease of E. faecalis counts after instrumentation and after PDT was compared using the Wilcoxon test, t-test and Kruskal Wallis test. A significant reduction of E. faecalis occurred after manual and rotary instrumentation and after PDT using the three combinations of light/photosensitizer (p<0.05). It may be concluded that both rotary and manual instrumentation reduced E. faecalis. Fuchsin with halogen light or LED irradiation and toluidine blue O with laser irradiation can be used to reduce E. faecalis in root canals of primary molars. PDT can be used as an adjuvant to conventional endodontic treatment.
authors.affiliation: PUC - Pontifical Catholic University of Campinas, Campinas, SP, Brazil.
authors.forename: Sérgio Luiz
authors.initials: SL
authors.lastname: Pinheiro
authors.affiliation: PUC - Pontifical Catholic University of Campinas, Campinas, SP, Brazil.
authors.forename: Josianne Neres da
authors.initials: JN
authors.lastname: Silva
authors.affiliation: PUC - Pontifical Catholic University of Campinas, Campinas, SP, Brazil.
authors.forename: Rafael Orro
authors.initials: RO
authors.lastname: Gonçalves
authors.affiliation: PUC - Pontifical Catholic University of Campinas, Campinas, SP, Brazil.
authors.forename: Karina Teixeira
authors.initials: KT
authors.lastname: Villalpando
mesh_terms: D058491:Bacterial Load; D001578:Benzopyrans / Q000494:pharmacology; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D008963:Molar; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011897:Random Allocation; D014048:Tolonium Chloride / Q000494:pharmacology; D014099:Toothbrushing
chemical_list: D001578:Benzopyrans; D017319:Photosensitizing Agents; C100026:fuscin; D014048:Tolonium Chloride