title: Anticancer activity of fungal L-asparaginase conjugated with zinc oxide nanoparticles.
abstract: Demand for developing novel delivery system for cancer treatment has increased due to the side effects present in intravenous injection of L-asparaginase. Nanoparticles are used for delivering the drugs to its destination in cancer cure. Nanobiocomposite of zinc oxide nanoparticles conjugated with L-asparaginase was produced by Aspergillus terreus and was confirmed using maximum UV-Vis absorption at 340 nm in the present work. The presence of functional groups like OH, C-H, -C=N and C=O on the surface of nanobiocomposite was found from Fourier transform infrared spectrum analysis. Size of the produced nanocomposite was found in the range of 28-63 nm using scanning electron microscope. The crystalline nature of the synthesized nanobiocomposites was confirmed by X-ray diffraction analysis. The presence of zinc oxide on synthesized nanobiocomposite was confirmed by energy dispersive spectrum analysis. The anti-cancerous nature of the synthesized zinc oxide conjugated L-asparaginase nanobiocomposite on MCF-7 cell line was studied using MTT assay. The viability of the MCF-7 cells was decreased to 35.02 % when it was treated with L-asparaginase conjugated zinc oxide nanobiocomposite. Hence it is proved that the synthesized nanobiocomposites of zinc oxide conjugated L-asparaginase has good anti-cancerous activity.
authors.affiliation: Department of Biotechnology, St. Joseph's College of Engineering, Chennai, 600 119, India, basg2004@gmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Baskar
authors.forename: J
authors.initials: J
authors.lastname: Chandhuru
authors.forename: K
authors.initials: K
authors.lastname: Sheraz Fahad
authors.forename: A S
authors.initials: AS
authors.lastname: Praveen
authors.forename: M
authors.initials: M
authors.lastname: Chamundeeswari
authors.forename: T
authors.initials: T
authors.lastname: Muthukumar
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D001215:Asparaginase / Q000008:administration & dosage / Q000737:chemistry*; D001230:Aspergillus / Q000201:enzymology; D045744:Cell Line, Tumor; D003460:Crystallization; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D008667:Metalloproteins; D001215:Asparaginase; D015034:Zinc Oxide