title: Comparative transcriptomic analysis reveals novel genes and regulatory mechanisms of Tetragenococcus halophilus in response to salt stress.
abstract: Tetragenococcus halophilus, a moderately halophilic Gram-positive bacterium, was isolated from Chinese style soy sauce. This species is a valuable resource for investigating salt tolerance mechanisms and improving salinity resistance in microorganisms. RNA-seq was used to sequence T. halophilus samples treated with 0 M (T1), 1 M (T2), and 3.5 M NaCl (T3). Comparative transcriptomic analyses of the different treatments were performed using gene ontology and Kyoto encyclopedia of genes and genome. The comparison of T1 and T2 by RNA-seq revealed that genes involved in transcription, translation, membrane system, and division were highly up-regulated under optimum salt condition. The comparison of T2 and T3 showed that genes related to heat shock proteins or the ATP-binding cassette transport systems were significantly up-regulated under maximum-salt condition. In addition, a considerable proportion of the significantly differently expressed genes identified in this study are novel. These data provide a crucial resource that may determine specific responses to salt stress in T. halophilus.
authors.affiliation: School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, China.
authors.forename: Licui
authors.initials: L
authors.lastname: Liu
authors.forename: Lifang
authors.initials: L
authors.lastname: Si
authors.forename: Xin
authors.initials: X
authors.lastname: Meng
authors.forename: Lixin
authors.initials: L
authors.lastname: Luo
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D056569:Enterococcaceae / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects* / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D009994:Osmolar Concentration; D014176:Protein Biosynthesis / Q000187:drug effects / Q000235:genetics; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D012965:Sodium Chloride / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D059467:Transcriptome / Q000187:drug effects* / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D006360:Heat-Shock Proteins; D012965:Sodium Chloride