title: Acid-sensing ion channels (ASICs) 2 and 4.2 are expressed in the retina of the adult zebrafish.
abstract: Acid-sensing ion channels (ASICs) are H(+)-gated, voltage-insensitive cation channels involved in synaptic transmission, mechanosensation and nociception. Different ASICs have been detected in the retina of mammals but it is not known whether they are expressed in adult zebrafish, a commonly used animal model to study the retina in both normal and pathological conditions. We study the expression and distribution of ASIC2 and ASIC4 in the retina of adult zebrafish and its regulation by light using PCR, in situ hybridization, western blot and immunohistochemistry. We detected mRNA encoding zASIC2 and zASIC4.2 but not zASIC4.1. ASIC2, at the mRNA or protein level, was detected in the outer nuclear layer, the outer plexiform layer, the inner plexiform layer, the retinal ganglion cell layer and the optic nerve. ASIC4 was expressed in the photoreceptors layer and to a lesser extent in the retinal ganglion cell layer. Furthermore, the expression of both ASIC2 and ASIC4.2 was down-regulated by light and darkness. These results are the first demonstration that ASIC2 and ASIC4 are expressed in the adult zebrafish retina and suggest that zebrafish could be used as a model organism for studying retinal pathologies involving ASICs.
authors.affiliation: Departamento de Morfología y Biología Celular, Grupo SINPOS Universidad de Oviedo, 33006, Oviedo, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Viña
authors.forename: V
authors.initials: V
authors.lastname: Parisi
authors.forename: C
authors.initials: C
authors.lastname: Sánchez-Ramos
authors.forename: R
authors.initials: R
authors.lastname: Cabo
authors.forename: M C
authors.initials: MC
authors.lastname: Guerrera
authors.forename: L M
authors.initials: LM
authors.lastname: Quirós
authors.forename: A
authors.initials: A
authors.lastname: Germanà
authors.forename: J A
authors.initials: JA
authors.lastname: Vega
authors.forename: O
authors.initials: O
authors.lastname: García-Suárez
mesh_terms: D062565:Acid Sensing Ion Channels / Q000096:biosynthesis*; D000818:Animals; D005136:Eye Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation / Q000502:physiology*; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000096:biosynthesis*
chemical_list: D062565:Acid Sensing Ion Channels; D005136:Eye Proteins; D029961:Zebrafish Proteins