title: Cell-free synthesis of SecYEG translocon as the fundamental protein transport machinery.
abstract: The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.
authors.affiliation: Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Matsubayashi
authors.forename: Yutetsu
authors.initials: Y
authors.lastname: Kuruma
authors.forename: Takuya
authors.initials: T
authors.lastname: Ueda
mesh_terms: D058045:Artificial Cells / Q000737:chemistry / Q000378:metabolism*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D000069497:Origin of Life; D010743:Phospholipids / Q000737:chemistry* / Q000302:isolation & purification; D021381:Protein Transport; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000069816:SEC Translocation Channels; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010743:Phospholipids; D011994:Recombinant Proteins; D000069816:SEC Translocation Channels; C065493:SecE protein, E coli; C088616:SecG protein, E coli; C057841:SecY protein, E coli; C412443:YIDC protein, E coli; C078167:OMPA outer membrane proteins; D012697:Serine Endopeptidases; C026515:type I signal peptidase