title: Transcriptome analysis of distinct Lindera glauca tissues revealed the differences in the unigenes related to terpenoid biosynthesis.
abstract: The Lindera glauca, an economically and ecologically important tree species, has emerged as a novel potential plant for the intensive studies of essential oil owing to its characteristic aroma and medicinal property in distinct tissues. However, the transcriptome information and molecular research on this species is still unknown to date. To reveal the formation and accumulation mechanism of essential oil in distinct L. glauca tissues, it is crucial to analyze transcriptome and to identify the full repertoire of potential unigenes involved in terpenoid biosynthesis. In this paper, the transcriptomes of the roots, sarcocarps, stems, leaves and kernels of L. glauca were analyzed for the first time by using short-read sequencing technology (Illumina). A total of 27.2GB valid reads (the average length=92.7bp) was obtained from distinct L. glauca tissues, and then assembled de novo into 264,831 unigenes by Trinity strategy (mean size=560.2bp). The resulting 98,141 unigenes (38%) of all the assembled unigenes were annotated in multiple public databases, of which 114 potential unigenes were identified to be involved in the terpenoid biosynthetic accumulation in L. glauca. Additionally, the differential expression profiles revealed 675, 697, 432, 1702 and 844 high tissue-specificity expressions of unigenes in the roots, sarcocarps, stems, leaves and kernels of L. glauca, respectively. Overall, these obtained comprehensive unigene resources will contribute to advance the research regarding the specific plant and more specifically discovery of genes participating in the terpenoid pathway and its regulation in specific tissues of the L. glauca, but also could help the understanding of the differential accumulation of secondary metabolites in distinct plant tissues.
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Niu
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Hou
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Fang
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Jiyong
authors.initials: J
authors.lastname: An
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: Denglong
authors.initials: D
authors.lastname: Ha
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Jigongshan National Nature Reserve, Xingyang 464133, China.
authors.forename: WenZhi
authors.initials: W
authors.lastname: Du
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Qi
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China.
authors.forename: Genan
authors.initials: G
authors.lastname: Liu
authors.affiliation: College of Biological Sciences and Biotechnology, College of Nature Conservation, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing 10083, China. Electronic address: szlin@bjfu.edu.cn.
authors.forename: Shanzhi
authors.initials: S
authors.lastname: Lin
mesh_terms: D030561:Databases, Nucleic Acid; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D017343:Genes, Plant / Q000502:physiology*; D027443:Lindera / Q000235:genetics / Q000378:metabolism*; D035261:Plant Components, Aerial / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D013729:Terpenes; D059467:Transcriptome / Q000502:physiology*
chemical_list: D013729:Terpenes
keywords: Differential profile; Distinct tissues; Illumina/Solexa; Lindera glauca; Terpenoid biosynthesis; Transcriptome analysis