title: On computation of calcium cycling anomalies in cardiomyocytes data.
abstract: Induced pluripotent stem cell (iPSC) lines derived from skin fibroblasts of patients suffering from cardiac disorders were differentiated to cardiomyocytes and used to generate a data set of Ca(2+) transients of 136 recordings. The objective was to separate normal signals for later medical research from abnormal signals. We constructed a signal analysis procedure to detect peaks representing calcium cycling in signals and another procedure to classify them into either normal or abnormal peaks. Using machine learning methods we classified signals into normal or abnormal signals on the basis of peak findings in them. We compared classification results obtained to those made visually by an expert biotechnologist who assessed the signals independent of the computer method. Classification accuracies of around 85% indicated high congruence between two modes denoting the high capability and usefulness of computer based processing for the present data.
authors.forename: Martti
authors.initials: M
authors.lastname: Juhola
authors.forename: Henry
authors.initials: H
authors.lastname: Joutsijoki
authors.forename: Kirsi
authors.initials: K
authors.lastname: Varpa
authors.forename: Jyri
authors.initials: J
authors.lastname: Saarikoski
authors.forename: Jyrki
authors.initials: J
authors.lastname: Rasku
authors.forename: Kati
authors.initials: K
authors.lastname: Iltanen
authors.forename: Jorma
authors.initials: J
authors.lastname: Laurikkala
authors.forename: Heikki
authors.initials: H
authors.lastname: Hyyrö
authors.forename: Jorge
authors.initials: J
authors.lastname: Avalos-Salguero
authors.forename: Harri
authors.initials: H
authors.lastname: Siirtola
authors.forename: Kirsi
authors.initials: K
authors.lastname: Penttinen
authors.forename: Katriina
authors.initials: K
authors.lastname: Aalto-Setälä
mesh_terms: D002118:Calcium / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D012815:Signal Processing, Computer-Assisted
chemical_list: D002118:Calcium