title: Synthesis of a highly dispersive sinapinic acid@graphene oxide (SA@GO) and its applications as a novel surface assisted laser desorption/ionization mass spectrometry for proteomics and pathogenic bacteria biosensing.
abstract: Graphene oxide (GO)-modified sinapinic acid (3,5-dimethoxy-4-hydroxycinnamic acid, SA) (SA@GO) was synthesized and characterized; it was then investigated as a new surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) for proteomics and pathogenic bacteria biosensing. SA@GO could effectively decrease the time necessary for sweet spotting searching, reducing the amount of organic matrix and solvent and enhance the sensitivity. SA@GO shows high performance as a matrix alone without the need to add trifluoroacetic acid (TFA). However, the analysis of the intact bacteria cells shows improvement in the signal intensity (2-5 fold) and offers a low limit of detection. All these analyses could be performed with low concentrations (1-10 fmol) and tiny volumes (0.5-1 μL). This study demonstrated that the exploration of new hybrid materials is pivotal to achieve high performance and high ionization. Because of the plane of GO, it assists protein-protein interactions that make it undergo softer ionization.
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 804, Taiwan.
authors.forename: Hani Nasser
authors.initials: HN
authors.lastname: Abdelhamid
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D003373:Coumaric Acids / Q000737:chemistry*; D005453:Fluorescence; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007768:Lactalbumin / Q000378:metabolism; D007834:Lasers; D009113:Muramidase / Q000378:metabolism; D053761:Nanocomposites / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D011552:Pseudomonas Infections / Q000175:diagnosis / Q000378:metabolism / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000378:metabolism / Q000382:microbiology; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014357:Trypsin / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; C073734:sinapinic acid; D006108:Graphite; D007768:Lactalbumin; D009113:Muramidase; D002480:Cellulase; D014357:Trypsin