title: Osteogenic responses to zirconia with hydroxyapatite coating by aerosol deposition.
abstract: Previously, we found that osteogenic responses to zirconia co-doped with niobium oxide (Nb2O5) or tantalum oxide (Ta2O5) are comparable with responses to titanium, which is widely used as a dental implant material. The present study aimed to evaluate the in vitro osteogenic potential of hydroxyapatite (HA)-coated zirconia by an aerosol deposition method for improved osseointegration. Surface analysis by scanning electron microscopy and x-ray diffraction proved that a thin as-deposited HA film on zirconia showed a shallow, regular, crater-like surface. Deposition of dense and uniform HA films was measured by SEM, and the contact angle test demonstrated improved wettability of the HA-coated surface. Confocal laser scanning microscopy indicated that MC3T3-E1 pre-osteoblast attachment did not differ notably between the titanium and zirconia surfaces; however, cells on the HA-coated zirconia exhibited a lower proliferation than those on the uncoated zirconia late in the culture. Nevertheless, ALP, alizarin red S staining, and bone marker gene expression analysis indicated good osteogenic responses on HA-coated zirconia. Our results suggest that HA-coating by aerosol deposition improves the quality of surface modification and is favorable to osteogenesis.
authors.affiliation: Department of Prosthodontics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea Department of Periodontology, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Cho
authors.affiliation: Department of Prosthodontics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Hong
authors.affiliation: Department of Molecular Genetics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea.
authors.forename: H
authors.initials: H
authors.lastname: Ryoo
authors.affiliation: Department of Advanced Materials Engineering, Sejong University, Seoul, Korea.
authors.forename: D
authors.initials: D
authors.lastname: Kim
authors.affiliation: R&D Center, Nano Business, IONES Co., Ltd., Gyeonggi-do, Korea.
authors.forename: J
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Prosthodontics, School of Dentistry and Dental Research Institute, BK21 Program, Seoul National University, Seoul, Korea proshan@snu.ac.kr.
authors.forename: J
authors.initials: J
authors.lastname: Han
mesh_terms: D016475:3T3 Cells; D000336:Aerosols; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000880:Anthraquinones; D002448:Cell Adhesion / Q000502:physiology; D049109:Cell Proliferation; D002516:Ceramics / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004396:Coloring Agents; D003764:Dental Materials / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009556:Niobium / Q000737:chemistry; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology*; D010087:Oxides / Q000737:chemistry; D013499:Surface Properties; D013635:Tantalum / Q000737:chemistry; D014025:Titanium / Q000737:chemistry; D017155:Wettability; D014961:X-Ray Diffraction; D015019:Yttrium / Q000737:chemistry; D015040:Zirconium / Q000737:chemistry*
chemical_list: D000336:Aerosols; D000880:Anthraquinones; D020099:Coated Materials, Biocompatible; D004396:Coloring Agents; D003764:Dental Materials; D010087:Oxides; C499362:yttria stabilized tetragonal zirconia; D009556:Niobium; C004468:Alizarin Red S; D015019:Yttrium; D013635:Tantalum; D017886:Durapatite; D015040:Zirconium; D014025:Titanium; D000469:Alkaline Phosphatase; C073337:niobium pentoxide; C078151:tantalum oxide
keywords: biomaterial(s); implant dentistry; osseointegration; osteogenesis; prosthetic dentistry; surface chemistry