title: Cucurbiturils as supramolecular inhibitors of DNA restriction by type II endonucleases.
abstract: Cucurbiturils (CB6 and CB7) were shown to inhibit the enzymatically catalyzed restriction of plasmids and linear DNA. This effect can be inverted by supramolecular masking of the macrocycles through competitive complexation with polyamines. These experiments provide supramolecular control of biocatalytic processes.
authors.affiliation: CIQSO-Center for Research in Sustainable Chemistry and Department of Chemical Engineering, Physical Chemistry and Organic Chemistry, University of Huelva, Campus El Carmen s/n, E-21071 Huelva, Spain. uwe.pischel@diq.uhu.es.
authors.forename: Cátia
authors.initials: C
authors.lastname: Parente Carvalho
authors.forename: Amir
authors.initials: A
authors.lastname: Norouzy
authors.forename: Vera
authors.initials: V
authors.lastname: Ribeiro
authors.forename: Werner M
authors.initials: WM
authors.lastname: Nau
authors.forename: Uwe
authors.initials: U
authors.lastname: Pischel
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002384:Catalysis; D002417:Cattle; D004247:DNA / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D004278:DNA, Superhelical / Q000737:chemistry; D004720:Endonucleases / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D047028:Macrocyclic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry; D000099246:Cucurbit(n)urils
chemical_list: D004247:DNA; D004278:DNA, Superhelical; D004720:Endonucleases; D004791:Enzyme Inhibitors; D047028:Macrocyclic Compounds; D010455:Peptides; D011073:Polyamines; D000099246:Cucurbit(n)urils