title: Highly sensitive method for specific, brief, and economical detection of glycoproteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis by the synthesis of a new hydrazide derivative.
abstract: A new hydrazide derivative was synthesized and used for the first time as a specific, brief, and economical probe to selectively visualize glycoproteins in 1-D and 2-D sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with high sensitivity. The detection limit of the newly developed staining method is 2- and 4-fold higher than that of the widely used Pro-Q Emerald 300 and 488 stains, respectively.
authors.affiliation: School of Pharmacy, Wenzhou Medical University , Wenzhou, Zhejiang 325035, P. R. China.
authors.forename: Weitao
authors.initials: W
authors.lastname: Cong
authors.forename: Ayi
authors.initials: A
authors.lastname: Zhou
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.forename: Jiayi
authors.initials: J
authors.lastname: Shen
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Weijian
authors.initials: W
authors.lastname: Ye
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xinliang
authors.initials: X
authors.lastname: Zhu
authors.forename: Jianjun
authors.initials: J
authors.lastname: Lin
authors.forename: Litai
authors.initials: L
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis*; D006834:Hydrazines / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data
chemical_list: D006023:Glycoproteins; D006834:Hydrazines