title: Isolation and genome characterization of a novel duck Tembusu virus with a 74 nucleotide insertion in the 3' non-translated region.
abstract: During investigations into the outbreak of duck Tembusu virus (DTMUV) infection in 2011 in China, a DTMUV strain (DTMUV-AH2011) was isolated from the affected ducks. The length of the genome of the DTMUV-AH2011 strain was found to be 11,064 nucleotides and to possess 10,278 nucleotides of one open reading frame (ORF), flanked by 94 nucleotides of the 5' non-translated region (NTR) and 692 nucleotides of the 3' NTR. In comparison with five fully sequenced TMUV genomes, the genome of DTMUV-AH2011 had a 74 nucleotide insertion in the 3' NTR. Comparison of the DTMUV-AH2011 fully deduced amino acid sequences with those of other Tembusu virus strains reported recently in China showed they had a highly conserved polyprotein precursor, sharing 98.9% amino acid identities, at least. The overall divergences of amino acid substitutions were randomly distributed among viral proteins except for the protein NS4B, the protein NS4B was unchanged. Knowledge of the biological characters of DTMUV and the potential role of the insertion in the 3' NTR in RNA replication will be useful for further studies of the mechanisms of virus replication and pathogenesis.
authors.affiliation: a Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine , Nanjing Agricultural University , Nanjing , China.
authors.forename: Jingman
authors.initials: J
authors.lastname: Wang
authors.forename: Weiting
authors.initials: W
authors.lastname: Liu
authors.forename: Gang
authors.initials: G
authors.lastname: Meng
authors.forename: Kangning
authors.initials: K
authors.lastname: Zhao
authors.forename: Jinyan
authors.initials: J
authors.lastname: Gu
authors.forename: Puyan
authors.initials: P
authors.lastname: Chen
authors.forename: Ruibing
authors.initials: R
authors.lastname: Cao
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks / Q000662:veterinary*; D004372:Ducks / Q000821:virology*; D005416:Flavivirus / Q000235:genetics*; D018177:Flavivirus Infections / Q000453:epidemiology* / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics*; D011201:Poultry Diseases / Q000821:virology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D020413:3' Untranslated Regions