title: Cytotoxic, antitumour-promoting and inhibition of protein denaturation effects of flavonoids, isolated from Potentilla evestita Th. Wolf.
abstract: This study was designed to evaluate the isolated flavonoids (chrysin 1, and umbelliferone 2) from Potentilla evestita for cytotoxic, antitumour-promoting and inhibition of protein denaturation activities. The results showed marked cytotoxic effect of compounds 1 and 2 in brine shrimp cytotoxic assay at various concentrations with LD50 of 34.5 and 31.8 mg/mL, respectively. In Epstein-Barr-virus early antigen activation assay, both compounds 1 and 2 illustrated significant antitumour-promoting effect with IC50 values of 462 and 308 mol ratio/32 pmol TPA, respectively. The cytotoxic and antitumour-promoting effects of compounds were strongly supported by inhibition of protein denaturation activity with IC50 of 119 and 112 μg/mL, respectively. In conclusion, both compounds possess strong cytotoxic, antitumour-promoting and inhibition of protein denaturation activities.
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.forename: Rehan
authors.initials: R
authors.lastname: Khan
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D001156:Artemia; D045744:Cell Line, Tumor; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50; D007928:Lethal Dose 50; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D031987:Potentilla / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D014468:Umbelliferones / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D010936:Plant Extracts; D014468:Umbelliferones; C043561:chrysin; C031477:7-hydroxycoumarin
keywords: Potentilla evestita; antitumour-promoter and inhibition of protein denaturation; chrysin and umbelliferone; cytotoxic