title: Saponins, especially platyconic acid A, from Platycodon grandiflorum reduce airway inflammation in ovalbumin-induced mice and PMA-exposed A549 cells.
abstract: We investigated the inhibitory effects of Platycodon grandiflorum root-derived saponins (Changkil saponins: CKS) on ovalbumin-induced airway inflammation in mice. CKS suppressed leukocytes number, IgE, Th1/Th2 cytokines, and MCP-1 chemokine secretion in bronchoalveolar lavage fluid. Also, ovalbumin-increased MUC5AC, MMP-2/9, and TIMP-1/-2 mRNA expression, NF-κB activation, leukocytes recruitment, and mucus secretion were inhibited by CKS treatment. Moreover, the active component of CKS, platyconic acid A (PA), suppressed PMA-induced MUC5AC mRNA expression (from 2.1 ± 0.2 to 1.1 ± 0.1) by inhibiting NF-κB activation (from 2.3 ± 0.2 to 1.2 ± 0.1) via Akt (from 3.7 ± 0.3 to 2.1 ± 0.2) (p < 0.01) in A549 cells. Therefore, we demonstrate that CKS or PA suppressed the development of respiratory inflammation, hyperresponsiveness, and remodeling by reducing allergic responses, and they may be potential herbal drugs for allergen-induced respiratory disease prevention.
authors.affiliation: Department of Toxicology, College of Pharmacy, Chungnam National University , Daejeon 305-764, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Choi
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Jin
authors.forename: Hyung Gyun
authors.initials: HG
authors.lastname: Kim
authors.forename: Chul Yung
authors.initials: CY
authors.lastname: Choi
authors.forename: Hyun Sun
authors.initials: HS
authors.lastname: Lee
authors.forename: Shi Yong
authors.initials: SY
authors.lastname: Ryu
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Chung
authors.forename: Young Jung
authors.initials: YJ
authors.lastname: Hwang
authors.forename: Yeon Ji
authors.initials: YJ
authors.lastname: Um
authors.forename: Tae Cheon
authors.initials: TC
authors.lastname: Jeong
authors.forename: Hye Gwang
authors.initials: HG
authors.lastname: Jeong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000276:immunology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000276:immunology; D051379:Mice; D008813:Mice, Inbred ICR; D055271:Mucin 5AC / Q000235:genetics / Q000276:immunology; D010047:Ovalbumin / Q000009:adverse effects; D018517:Plant Roots / Q000737:chemistry; D031238:Platycodon / Q000737:chemistry*; D012503:Saponins / Q000008:administration & dosage*; D013755:Tetradecanoylphorbol Acetate / Q000009:adverse effects; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000276:immunology; D014315:Triterpenes / Q000008:administration & dosage*
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; C524679:Muc5ac protein, mouse; D055271:Mucin 5AC; D012503:Saponins; D019715:Tissue Inhibitor of Metalloproteinase-1; D014315:Triterpenes; C576414:platyconic acid; D010047:Ovalbumin; D020778:Matrix Metalloproteinase 2; D013755:Tetradecanoylphorbol Acetate
keywords: NF-κB; Platycodon grandiflorum; airway inflammation; platyconic acid A; saponins