title: Adhesion and growth of human bone marrow mesenchymal stem cells on precise-geometry 3D organic-inorganic composite scaffolds for bone repair.
abstract: Engineering biomaterial scaffolds that promote attachment and growth of mesenchymal stem cells in three dimensions is a crucial parameter for successful bone tissue engineering. Towards this direction, a lot of research effort has focused recently into the development of three-dimensional porous scaffolds, aiming to elicit positive cellular behavior. However, the fabrication of three-dimensional tissue scaffolds with a precise geometry and complex micro- and nano-features, supporting cell in-growth remains a challenge. In this study we report on a positive cellular response of human bone marrow-derived (BM) mesenchymal stem cells (MSCs) onto hybrid material scaffolds consisting of methacryloxypropyl trimethoxysilane, zirconium propoxide, and 2-(dimethylamino)ethyl methacrylate (DMAEMA). First, we use Direct fs Laser Writing, a 3D scaffolding technology to fabricate the complex structures. Subsequently, we investigate the morphology, viability and proliferation of BM-MSCs onto the hybrid scaffolds and examine the cellular response from different donors. Finally, we explore the effect of the materials' chemical composition on cell proliferation, employing three different material surfaces: (i) a hybrid consisting of methacryloxypropyl trimethoxysilane, zirconium propoxide and 50mol% DMAEMA, (ii) a hybrid material comprising methacryloxypropyl trimethoxysilane and zirconium propoxide, and (iii) a purely organic polyDMAEMA. Our results show a strong adhesion of BM-MSCs onto the hybrid material containing 50% DMAEMA from the first 2h after seeding, and up to several days, and a proliferation increase after 14 and 21days, similar to the polystyrene control, independent of cell donor. These findings support the potential use of our proposed cell-material combination in bone tissue engineering.
authors.affiliation: Department of Materials Science and Technology, University of Crete, Greece; Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), Greece. Electronic address: mchatzin@materials.uoc.gr.
authors.forename: Maria
authors.initials: M
authors.lastname: Chatzinikolaidou
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), Greece.
authors.forename: Sima
authors.initials: S
authors.lastname: Rekstyte
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), Greece.
authors.forename: Paulius
authors.initials: P
authors.lastname: Danilevicius
authors.affiliation: Hematology Laboratory, School of Medicine, University of Crete, Greece.
authors.forename: Charalampos
authors.initials: C
authors.lastname: Pontikoglou
authors.affiliation: Hematology Laboratory, School of Medicine, University of Crete, Greece.
authors.forename: Helen
authors.initials: H
authors.lastname: Papadaki
authors.affiliation: Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Farsari
authors.affiliation: Department of Materials Science and Technology, University of Crete, Greece; Institute of Electronic Structure and Laser (IESL), Foundation for Research and Technology Hellas (FORTH), Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Vamvakaki
mesh_terms: D000433:1-Propanol; D001842:Bone and Bones; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D049109:Cell Proliferation; D048091:Guided Tissue Regeneration; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000502:physiology*; D008689:Methacrylates; D018613:Microscopy, Confocal / Q000379:methods; D008855:Microscopy, Electron, Scanning; D012821:Silanes; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D015040:Zirconium
chemical_list: C542237:3-methacryloxypropyltrimethoxysilane; D008689:Methacrylates; D012821:Silanes; D000433:1-Propanol; D015040:Zirconium; C049840:2-(dimethylamino)ethyl methacrylate
keywords: 3D scaffold fabrication; Cell adhesion and proliferation; Cell variability; Human bone marrow mesenchymal stem cells; Hybrid material