title: Rapid and sensitive identification of the herbal tea ingredient Taraxacum formosanum using loop-mediated isothermal amplification.
abstract: Taraxacum formosanum (TF) is a medicinal plant used as an important component of health drinks in Taiwan. In this study, a rapid, sensitive and specific loop-mediated isothermal amplification (LAMP) assay for authenticating TF was established. A set of four specific LAMP primers was designed based on the nucleotide sequence of the internal transcribed spacer 2 (ITS2) nuclear ribosomal DNA (nrDNA) of TF. LAMP amplicons were successfully amplified and detected when purified genomic DNA of TF was added in the LAMP reaction under isothermal condition (65 °C) within 45 min. These specific LAMP primers have high specificity and can accurately discriminate Taraxacum formosanum from other adulterant plants; 1 pg of genomic DNA was determined to be the detection limit of the LAMP assay. In conclusion, using this novel approach, TF and its misused plant samples obtained from herbal tea markets were easily identified and discriminated by LAMP assay for quality control.
authors.affiliation: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40402, Taiwan. just_dfm@hotmail.com.
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Lai
authors.affiliation: Department & Institute of Pharmacology, National Yang-Ming University, Taipei 11221, Taiwan. rich720925@yahoo.com.tw.
authors.forename: Jung
authors.initials: J
authors.lastname: Chao
authors.affiliation: Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. linmk@mail.cmu.edu.tw.
authors.forename: Ming-Kuem
authors.initials: MK
authors.lastname: Lin
authors.affiliation: Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. wtchang@mail.cmu.edu.tw.
authors.forename: Wen-Te
authors.initials: WT
authors.lastname: Chang
authors.affiliation: Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. whpeng@mail.cmu.edu.tw.
authors.forename: Wen-Huang
authors.initials: WH
authors.lastname: Peng
authors.affiliation: Department of Bioresources, Da-Yeh University, Changhua 51591, Taiwan. fcsun@mail.dyu.edu.tw.
authors.forename: Fang-Chun
authors.initials: FC
authors.lastname: Sun
authors.affiliation: Management Center, Department of Medical Research and Development, Show Chwan Health Care Sysytem, Changhua 51951, Taiwan. mengshiunn@gmail.com.
authors.forename: Meng-Shiunn
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Chinese Pharmaceutical Science and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan. leemengshiou@mail.cmu.edu.tw.
authors.forename: Meng-Shiou
authors.initials: MS
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D016002:Discriminant Analysis; D017343:Genes, Plant; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D016415:Sequence Alignment; D031205:Taraxacum / Q000378:metabolism*; D013696:Temperature
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal