title: Low concentrations of cylindrospermopsin induce increases of reactive oxygen species levels, metabolism and proliferation in human hepatoma cells (HepG2).
abstract: Human hepatoma cells (HepG2) were exposed to purified cylindrospermopsin (CYN), a potent toxicant for eukaryotic cells produced by several cyanobacteria. CYN was not toxic at concentrations up to 10 μgl(-1), leading to increased viability and metabolism in cells cultured with 10% fetal bovine serum (FBS). Reduction of FBS concentration to 2% and induction of cytochrome P450 (CYP) isoforms were performed in order to make xenobiotic-metabolizing capacity of HepG2 cells closest to that of 'normal' cells. HepG2 cells proliferated less after CYPs-induction, and this induction has lead to similar results of non-induced cells, except for few individual parameters such lipid peroxidation. Foremost, low concentrations of CYN (below or equal 10 μgl(-1)) have induced HepG2 cells proliferation and metabolism increase, which was not expected.
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: samliebel@yahoo.com.br.
authors.forename: Samuel
authors.initials: S
authors.lastname: Liebel
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil.
authors.forename: Ciro Alberto
authors.initials: CA
authors.lastname: de Oliveira Ribeiro
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Valéria Freitas
authors.initials: VF
authors.lastname: de Magalhães
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Ilha do Fundão, Universidade Federal do Rio de Janeiro, CEP 21949-900, Rio de Janeiro, RJ, Brazil.
authors.forename: Rodrigo de Cássio
authors.initials: Rde C
authors.lastname: da Silva
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil.
authors.forename: Stéfani Cibele
authors.initials: SC
authors.lastname: Rossi
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil.
authors.forename: Marco Antônio Ferreira
authors.initials: MA
authors.lastname: Randi
authors.affiliation: Departamento de Biologia Celular, Universidade Federal do Paraná, Cx. Postal 19031, CEP 81.531-990, Curitiba, PR, Brazil. Electronic address: filipak@ufpr.br.
authors.forename: Francisco
authors.initials: F
authors.lastname: Filipak Neto
mesh_terms: D000470:Alkaloids; D001427:Bacterial Toxins; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival; D000087522:Cyanobacteria Toxins; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D056945:Hep G2 Cells; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014498:Uracil / Q000031:analogs & derivatives* / Q000633:toxicity; D015262:Xenobiotics / Q000378:metabolism
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D017382:Reactive Oxygen Species; D015262:Xenobiotics; C089595:cylindrospermopsin; D014498:Uracil; D003577:Cytochrome P-450 Enzyme System
keywords: CYPs; Cell lineage; Cyanotoxin; Cylindrospermopsin; HepG2