title: The yeast BDF1 regulates endocytosis via LSP1 under salt stress.
abstract: Bromodomain-containing transcription factor, a kind of important regulating protein, can recognize and bind to acetylated histone. The homologous genes, BDF1 and BDF2, in Saccharomyces cerevisiae, respectively, encode a bromodomain-containing transcription factor. Previously study has demonstrated that both BDF1 and BDF2 participate in yeast salt stress response. Bdf1p deletion cells are sensitive to salt stress and this phenotype is suppressed by its homologue BDF2 in a dosage-dependent manner. In this study, we show that the histone deacetylase SIR2 over-expression enhanced dosage-dependent compensation of BDF2. SIR2 over-expression induced a global transcription change, and 1959 gene was down-regulated. We deleted some of the most significant down-regulated genes and did the spot assay. The results revealed that LSP1, an upstream component of endocytosis pathway, and CIN5, a transcription factor that mediates cellular resistance to stresses, can enhance salt resistance of bdf1∆. Further analysis demonstrated that under salt stress the endocytosis is over-activated in bdf1∆ but was recovered in bdf1∆ lsp1∆. To our best knowledge, this is the first report that the transcription factor Bdf1p regulates endocytosis under salt stress via LSP1, a major component of eisosomes that regulate the sites of endocytosis.
authors.affiliation: Shandong Medicinal Biotechnology Centre, Shandong Academy of Medical Sciences, Jinan, 250062, China.
authors.forename: Jiafang
authors.initials: J
authors.lastname: Fu
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Mingpeng
authors.initials: M
authors.lastname: Wang
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhaojie
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
mesh_terms: D004705:Endocytosis; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005816:Genetic Complementation Test; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D012492:Salts / Q000633:toxicity*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D056565:Sirtuin 2 / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000092468:Bromodomain Containing Proteins
chemical_list: D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D012492:Salts; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D056565:Sirtuin 2; D014157:Transcription Factors; C091342:BDF1 protein, S cerevisiae; D000092468:Bromodomain Containing Proteins; C487328:LSP1 protein, S cerevisiae; C087778:SIR2 protein, S cerevisiae