title: The effects of social defeat on behavior and dopaminergic markers in mice.
abstract: The present study investigated the effects of chronic social defeat stress on several behavioral parameters, and the expression of dopaminergic markers, i.e., dopamine D1 receptors (D1Rs), dopamine D2 receptors (D2Rs), and dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein-32 (DARPP-32), in the prefrontal cortex (PFC), amygdala (AMY), and hippocampus (HIP) of mouse brains. After 10days of social defeat stress, the defeated mice were divided into two groups: one group underwent a series of behavioral tests. The other group was sacrificed on the 11th day and tissue samples were collected for Western blotting. The behavioral tests comprised tests of locomotion, light/dark preference, social interaction, as well as the novel object recognition test (NORT), Morris water maze, and forced swimming test (FST). We measured the expression of D1Rs, D2Rs, total DARPP-32, phospho-Thr34 or Thr75-DARPP-32 using Western blotting. The defeated mice showed increased anxiety- and depression-like behaviors, and impaired cognition. No significant differences in D1Rs and D2Rs expression were shown between defeated and control mice in any area studied. A significantly increased expression in total DARPP-32, and phospho-DARPP-32 was observed in the PFC or AMY of defeated mice. These data suggest that alterations in dopaminergic markers may be involved in anxiety- and depression-like behaviors, and cognitive impairment induced by social defeat stress.
authors.affiliation: Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: hongmeiab@gmail.com.
authors.forename: H-M
authors.initials: HM
authors.lastname: Jin
authors.affiliation: Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: sushma_shrestha17@hotmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Shrestha Muna
authors.affiliation: Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: tarique_rb@yahoo.com.
authors.forename: T R
authors.initials: TR
authors.lastname: Bagalkot
authors.affiliation: Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: cuiyin61@gmail.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Neurology, Chonbuk National University Medical School, Jeonju, Republic of Korea. Electronic address: binod3aug@gmail.com.
authors.forename: B K
authors.initials: BK
authors.lastname: Yadav
authors.affiliation: Department of Psychiatry, Chonbuk National University Medical School, Jeonju, Republic of Korea; Research Institute of Clinical Medicine of Chonbuk National University, Biomedical Research Institute of Chonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: chungyc@jbnu.ac.kr.
authors.forename: Y-C
authors.initials: YC
authors.lastname: Chung
mesh_terms: D000818:Animals; D001007:Anxiety / Q000503:physiopathology*; D001921:Brain / Q000503:physiopathology*; D002908:Chronic Disease; D003071:Cognition / Q000502:physiology*; D003863:Depression / Q000503:physiopathology*; D004291:Dominance-Subordination; D051937:Dopamine and cAMP-Regulated Phosphoprotein 32 / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology; D010766:Phosphorylation; D017447:Receptors, Dopamine D1 / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D013315:Stress, Psychological / Q000503:physiopathology*
chemical_list: D051937:Dopamine and cAMP-Regulated Phosphoprotein 32; C497929:Ppp1r1b protein, mouse; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2
keywords: behavioral tests; dopamine D(1) receptors; dopamine D2 receptors; dopamine and cyclic adenosine 3′,5′-monophosphate-regulated phosphoprotein-32 (DARPP-32); social defeat