title: Plain silver surface plasmon resonance for microarray application.
abstract: The application scope of surface plasmon resonance (SPR) and SPR imaging (SPRi) is rapidly growing, and tools such as high-performance and low-cost slides could enable more rapid growth of the field. We describe herein a novel silver slide, addressing the inherent instability of plain silver structure by improving adhesion between the glass substrate and the silver layer with a thin buffer layer of gold. Covered by a self-assembled monolayer (SAM) only, SPR characteristics of the slide remain steady for more than 3 months under regular storage. In a bioassay, the slide substantiates the predicted nearly 100% sensitivity improvement over gold slides and exhibits exceptional performance stability as determined by sensitivity and resolution measurements during the extended 40,000 s multicycle experiment. We demonstrate the suitability of this new slide for large-area SPRi, describing analysis results from a 1 296-ligand protein microarray. We predict this slide structure will provide a stable, high-sensitivity solution for high-throughput SPRi applications and other surface analysis platforms.
authors.affiliation: National Center for NanoScience and Technology , Beijing 100190, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Cheng
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Wang
authors.forename: Doreen E
authors.initials: DE
authors.lastname: Gillespie
authors.forename: Christopher
authors.initials: C
authors.lastname: Lausted
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zheng
authors.forename: Mo
authors.initials: M
authors.lastname: Yang
authors.forename: Jinsong
authors.initials: J
authors.lastname: Zhu
mesh_terms: D004867:Equipment Design; D008024:Ligands; D040081:Protein Array Analysis / Q000295:instrumentation*; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
chemical_list: D008024:Ligands; D012834:Silver