title: The effect of isorhamnetin glycosides extracted from Opuntia ficus-indica in a mouse model of diet induced obesity.
abstract: A diet rich in polyphenols can ameliorate some metabolic alterations associated with obesity and type 2 diabetes. Opuntia ficus-indica (OFI) is a plant rich in isorhamnetin glycosides and is highly consumed in Mexico. The purpose of this research was to determine the metabolic effect of an OFI extract on a mouse model of diet-induced obesity and in isolated pancreatic islets. OFI extract was added to a high fat (HF) diet at a low (0.3%) or high (0.6%) dose and administered to C57BL/6 mice for 12 weeks. Mice fed the HF diet supplemented with the OFI extract gained less body weight and exhibited significantly lower circulating total cholesterol, LDL cholesterol and HDL cholesterol compared to those fed the HF diet alone. The HF-OFI diet fed mice presented lower glucose and insulin concentration than the HF diet fed mice. However, the HF-OFI diet fed mice tended to have higher insulin concentration than control mice. The OFI extract stimulated insulin secretion in vitro, associated with increased glucose transporter 2 (GLUT2) and peroxisome proliferator-activated receptor gamma (PPARγ) mRNA content. Furthermore, the OFI extract improved glucose tolerance, and additionally increased energy expenditure. These metabolic improvements were associated with reduced adipocyte size, increased hepatic IRS1 tyr-608 and S6 K thr-389 phosphorylation. OFI isorhamnetin glycosides also diminished the hepatic lipid content associated with reduced mRNA expression of the endoplasmic reticulum stress markers and lipogenic enzymes and increased mRNA expression of genes related to fatty acid oxidation. Overall, the OFI extract prevented the development of metabolic abnormalities associated with diet-induced obesity.
authors.affiliation: Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849 Monterrey, NL, México.
authors.forename: César
authors.initials: C
authors.lastname: Rodríguez-Rodríguez
authors.forename: Nimbe
authors.initials: N
authors.lastname: Torres
authors.forename: Janet A
authors.initials: JA
authors.lastname: Gutiérrez-Uribe
authors.forename: Lilia G
authors.initials: LG
authors.lastname: Noriega
authors.forename: Iván
authors.initials: I
authors.lastname: Torre-Villalvazo
authors.forename: Ana M
authors.initials: AM
authors.lastname: Leal-Díaz
authors.forename: Marilena
authors.initials: M
authors.lastname: Antunes-Ricardo
authors.forename: Claudia
authors.initials: C
authors.lastname: Márquez-Mota
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ordaz
authors.forename: Rocío A
authors.initials: RA
authors.lastname: Chavez-Santoscoy
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Serna-Saldivar
authors.forename: Armando R
authors.initials: AR
authors.lastname: Tovar
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D004734:Energy Metabolism; D005786:Gene Expression Regulation; D051273:Glucose Transporter Type 2 / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D006027:Glycosides / Q000008:administration & dosage / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use*; D006943:Hyperglycemia / Q000209:etiology / Q000517:prevention & control; D006949:Hyperlipidemias / Q000209:etiology / Q000517:prevention & control; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D029747:Opuntia / Q000737:chemistry*; D047495:PPAR gamma / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D018547:Plant Stems / Q000737:chemistry; D011794:Quercetin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification / Q000627:therapeutic use; D011897:Random Allocation; D017208:Rats, Wistar; D046509:Tissue Culture Techniques
chemical_list: D019440:Anti-Obesity Agents; D051273:Glucose Transporter Type 2; D006027:Glycosides; D007328:Insulin; D047495:PPAR gamma; D010936:Plant Extracts; C495683:Slc2a2 protein, rat; C047368:3-methylquercetin; D011794:Quercetin