title: MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells.
abstract: Fyn kinase is implicated in prostate cancer. We illustrate the role of miR-125a-3p in cellular pathways accounted for motility and migration of prostate cancer cells, probably through its regulation on Fyn expression and Fyn-downstream proteins. Prostate cancer PC3 cells were transiently transfected with empty miR-Vec (control) or with miR-125a-3p. Overexpression of miR-125a-3p reduced migration of PC3 cells and increased apoptosis. Live cell confocal imaging indicated that overexpression of miR-125a-3p reduced the cells' track speed and length and impaired phenotype. Fyn, FAK and paxillin, displayed reduced activity following miR-125a-3p overexpression. Accordingly, actin rearrangement and cells' protrusion formation were impaired. An inverse correlation between miR-125a-3p and Gleason score was observed in human prostate cancer tissues. Our study demonstrated that miR-125a-3p may regulate migration of prostate cancer cells.
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.|This work was performed in partial fulfillment of the requirements for a Ph.D. degree of Lihi Ninio-Many, Sackler Faculty of Medicine, Tel Aviv University, Israel.
authors.forename: Lihi
authors.initials: L
authors.lastname: Ninio-Many
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Hadas
authors.initials: H
authors.lastname: Grossman
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Mattan
authors.initials: M
authors.lastname: Levi
authors.affiliation: Department of Pathology, Rabin Medical Center, Beilinson Campus, Petah-Tiqva, Israel.
authors.forename: Sofia
authors.initials: S
authors.lastname: Zilber
authors.affiliation: Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: Ilan
authors.initials: I
authors.lastname: Tsarfaty
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Noam
authors.initials: N
authors.lastname: Shomron
authors.affiliation: Department of Pathology, Rabin Medical Center, Beilinson Campus, Petah-Tiqva, Israel.
authors.forename: Anna
authors.initials: A
authors.lastname: Tuvar
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Dana
authors.initials: D
authors.lastname: Chuderland
authors.affiliation: Institute of Oncology, Davidoff Center, Rabin Medical Center, Beilinson Campus, Petah-Tiqva, and Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: Salomon M
authors.initials: SM
authors.lastname: Stemmer
authors.affiliation: Institute of Oncology, Davidoff Center, Rabin Medical Center, Beilinson Campus, Petah-Tiqva, and Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: Irit
authors.initials: I
authors.lastname: Ben-Aharon
authors.affiliation: Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Ruth
authors.initials: R
authors.lastname: Shalgi
keywords: EMT; Fyn; actin cytoskeleton; live imaging; miR-125a-3p; migration; prostate cancer