title: MicroRNA-221 accelerates the proliferation of laryngeal cancer cell line Hep-2 by suppressing Apaf-1.
abstract: Laryngeal cancer is one of the most commonly occurring malignant cancers of the head and neck region. In the present study, we investigated the roles of miR-221 in laryngeal squamous cell carcinoma cell line, Hep-2. We examined the function and mechanism of miR-221 in Hep-2 cells using techniques of cell biology and molecular pathology, such as western blotting, quantitative PCR, immunohistochemical staining and flow cytometry. Using a luciferase assay, the apoptotic protease activating factor-1 (Apaf-1) mRNA 3'-UTR was shown to have complementary binding sites using bioinformatics prediction software including TargetScan, PicTar and miRanda. In conclusion, our results showed that miR-221 inhibition caused elevated expression levels of the Apaf-1 apoptotic pathway proteins caspase-3, -8 and -9. miR-221 may therefore be used as a novel therapeutic target for laryngeal cancer.
authors.affiliation: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Zhao
authors.affiliation: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Li-Yin
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, Shengjing Hospital, China Medical University, Heping, Shenyang, Liaoning 110004, P.R. China.
authors.forename: Wen-Yue
authors.initials: WY
authors.lastname: Ji
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D053477:Apoptotic Protease-Activating Factor 1 / Q000096:biosynthesis* / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000096:biosynthesis; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous
chemical_list: D053477:Apoptotic Protease-Activating Factor 1; D053148:Caspase 3; D053181:Caspase 8; D053453:Caspase 9; D050760:Cyclin-Dependent Kinase Inhibitor p27; D035683:MicroRNAs; C506592:APAF1 protein, human; C436291:CDKN1B protein, human; C525709:MIR221, human