title: Development of a liquid chromatography with mass spectrometry method for the determination of gelsemine in rat plasma and tissue: Application to a pharmacokinetic and tissue distribution study.
abstract: Gelsemine from Gelsemium elegans Benth is a potential anesthetic and analgesic agent with no physical dependence and opiate addiction. This study was aimed at developing an ultrafast liquid chromatography coupled to tandem mass spectrometry method to quantify gelsemine in rat plasma and tissues. Plasma and tissues were processed with acetonitrile precipitation, and dendrobine was chosen as the internal standard. Sample separation was performed on an ACQUITY HSS T3 column. The mobile phase consisted of acetonitrile and 0.1% formic acid aqueous solution. Multiple reactions monitoring mode was utilized to detect the compounds of interest. The mass spectrometer was operated in the positive ion mode for detection. The MS/MS ion transitions monitored were m/z 323.2→70.5 for gelsemine and 264.2→108.05 for dendrobine, respectively. The calibration curves were linear over the range of 1-500 ng/mL in all biological matrices. The lower limit of quantification for rats plasma and tissues was 1.0 ng/mL. The values for inter- and intraday precision and accuracy were well within the ranges acceptable (< 15%). It was successfully applied to the pharmacokinetic and tissue distribution studies of gelsemine after intravenous doses of 5, 2, and 0.5 mg/kg in rats. These data of gelsemine would be useful for clinical application and further development.
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Shuping
authors.initials: S
authors.lastname: Hu
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Yang
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Shen
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zheng
authors.forename: Kexin
authors.initials: K
authors.lastname: Huang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xiang
mesh_terms: D000470:Alkaloids / Q000097:blood / Q000493:pharmacokinetics*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000493:pharmacokinetics*; D031450:Gelsemium / Q000737:chemistry*; D007668:Kidney / Q000737:chemistry; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013270:Stomach / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal; C063230:gelsemine
keywords: Gelsemine; Pharmacokinetics; Rat; Tissue distribution; Ultrafast liquid chromatography