title: Upconversion nanoparticle-based fluorescence resonance energy transfer assay for organophosphorus pesticides.
abstract: This paper reports a novel nanosensor for organophosphorus pesticides based on the fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs). The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Under the optimized conditions, the logarithm of the pesticides concentration was proportional to the inhibition efficiency. The detection limits of parathion-methyl, monocrotophos and dimethoate reached 0.67, 23, and 67 ng/L, respectively. Meanwhile, the biosensor shows good sensitivity, stability, and could be successfully applied to detection of OPs in real food samples, suggesting the biosensor has potentially extensive application clinic diagnoses assays.
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Long
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China. Electronic address: zhangyy@hunnu.edu.cn.
authors.forename: Youyu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010575:Pesticides / Q000737:chemistry / Q000302:isolation & purification*; D013860:Thiocholine / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D010575:Pesticides; D013860:Thiocholine; D006046:Gold; D000110:Acetylcholinesterase
keywords: Fluorescence resonance energy transfer; Gold nanoparticle; Organophosphorus pesticides; Upconversion nanoparticles