title: Effect of radiation on cytotoxicity, apoptosis and cell cycle arrest of human osteosarcoma MG-63 induced by a ruthenium(II) complex.
abstract: Radiation has large influence on the cytotoxicity, apoptosis and cell cycle arrest. The bioactivity of ruthenium(II) complex [Ru(dmb)2(DBHIP)](ClO4)2 (Ru1) (DBHIP=2-(3,5-dibromo-4-hydroxylphenyl)imidazo[4,5-f][1,10]phenanthroline) was investigated in the absence and presence of radiation. The cytotoxicity of Ru1 against MG-63 cells was evaluated by CCK-8 method. Ru1 shows high cytotoxicity upon radiation. Radiation can enhance the cytotoxicity of Ru1 on MG-63 cells. The apoptosis was studied by Hoechst 33258 staining method and flow cytometry. The reactive oxygen species, mitochondrial membrane potential, cell cycle arrest and western blot analysis were investigated in detail. The complex induces the apoptosis in MG-63 cells through ROS-mediated mitochondrial dysfunction pathway.
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Si-Hong
authors.initials: SH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Taishan City People's Hospital, Taishan 529200, PR China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Kun-Kang
authors.initials: KK
authors.lastname: Deng
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, PR China. Electronic address: qifengguoqfg@126.com.
authors.forename: Qi-Feng
authors.initials: QF
authors.lastname: Guo
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy; D059447:Cell Cycle Checkpoints / Q000187:drug effects* / Q000528:radiation effects; D045744:Cell Line, Tumor; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects / Q000528:radiation effects; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy; D010618:Phenanthrolines / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012428:Ruthenium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D010618:Phenanthrolines; D017382:Reactive Oxygen Species; D012428:Ruthenium
keywords: Apoptosis; Cell cycle arrest; Cytotoxicity in vitro; Mitochondrial membrane potential; Reactive oxygen species; Ruthenium(II) complex