title: The Arabidopsis leucine-rich repeat receptor-like kinase MUSTACHES enforces stomatal bilateral symmetry in Arabidopsis.
abstract: Stomata display a mirror-like symmetry that is adaptive for shoot/atmosphere gas exchange. This symmetry includes the facing guard cells around a lens-shaped and bilaterally symmetric pore, as well as radially arranged microtubule arrays that primarily originate at the pore and then grow outwards. Mutations in MUSTACHES (MUS), which encodes a leucine-rich repeat receptor-like kinase, disrupt this symmetry, resulting in defects ranging from skewed pores and abnormally focused and depolarized radial microtubule arrays, to paired guard cells that face away from each other, or a severe loss of stomatal shape. Translational MUSproMUS:tripleGFP fusions are expressed in cell plates in most cells types in roots and shoots, and cytokinesis and cell plates are mostly normal in mus mutants. However, in guard mother cells, which divide and then form stomata, MUS expression is notably absent from new cell plates, and instead is peripherally located. These results are consistent with a role for MUS in enforcing wall building and cytoskeletal polarity at the centre of the developing stoma via signalling from the vicinity of the guard cell membrane.
authors.affiliation: Botany Department, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4.
authors.forename: Sandra
authors.initials: S
authors.lastname: Keerthisinghe
authors.forename: Jeannette A
authors.initials: JA
authors.lastname: Nadeau
authors.forename: Jessica R
authors.initials: JR
authors.lastname: Lucas
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Fred D
authors.initials: FD
authors.lastname: Sack
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D002473:Cell Wall / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D017930:Genes, Reporter; D000090263:Leucine-Rich Repeat Proteins; D008870:Microtubules / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D054046:Plant Stomata / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D017346:Protein Serine-Threonine Kinases; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins
chemical_list: D029681:Arabidopsis Proteins; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D020794:Receptor Protein-Tyrosine Kinases; C000603558:MUSTACHES protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: Arabidopsis thaliana; At1g75640; bilateral symmetry; microtubule dynamics; microtubules; stomatal morphogenesis