title: Assessment of pesticide residues and gene expression in common carp exposed to atrazine and chlorpyrifos: Health risk assessments.
abstract: This study assessed the impacts of atrazine (ATR), chlorpyrifos (CPF) and combined ATR/CPF exposure on the kidney of common carp (Cyprinus carpio L.). The carp were sampled after a 40-d exposure to CPF and ATR, individually or in combination, followed by a 40-d recovery to measure the expression levels of heat shock proteins genes (HSP60, HSP70 and HSP90) and pesticide residues in the kidney tissue. The results revealed that the mRNA and protein levels of HSP60, HSP70 and HSP90 were induced in the kidney of common carp by ATR, CPF, and ATR/CPF mixture. The accumulated amounts of ATR, CPF, and their metabolites in the kidney tissues exhibited dose-dependency. These results exhibited that increasing concentration of ATR and CPF in the environment causes considerable stress for common carp, suggesting that the expression levels of HSP60, HSP70 and HSP90 may act as potential biomarkers for assessing the environmental ATR and CPF risk for carp.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China; Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
authors.forename: Houjuan
authors.initials: H
authors.lastname: Xing
authors.affiliation: Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Academy of Agricultural Sciences of Heilongjiang Province, 368 Xuefu Road, Xiangfang District, Harbin 150086, PR China.
authors.forename: Hongda
authors.initials: H
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China. Electronic address: shiwenxu@neau.edu.cn.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Xu
mesh_terms: D000818:Animals; D001280:Atrazine / Q000378:metabolism / Q000633:toxicity*; D015153:Blotting, Western; D002347:Carps; D004390:Chlorpyrifos / Q000378:metabolism / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D010573:Pesticide Residues / Q000378:metabolism / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D018570:Risk Assessment; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D010573:Pesticide Residues; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D004390:Chlorpyrifos; D001280:Atrazine
keywords: Fish; Heat shock proteins; Pesticide residues; Western blot; mRNA