title: Three-dimensional visualization of the Autographa californica multiple nucleopolyhedrovirus occlusion-derived virion envelopment process gives new clues as to its mechanism.
abstract: Baculoviruses produce two virion phenotypes, occlusion-derived virion (ODV) and budded virion (BV). ODV envelopment occurs in the nucleus. Morphogenesis of the ODV has been studied extensively; however, the mechanisms underlying microvesicle formation and ODV envelopment in nuclei remain unclear. In this study, we used electron tomography (ET) together with the conventional electron microscopy to study the envelopment of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) ODV. Our results demonstrate that not only the inner but also the outer nuclear membrane can invaginate and vesiculate into microvesicles and that intranuclear microvesicles are the direct source of the ODV membrane. Five main events in the ODV envelopment process are summarized, from which we propose a model to explain this process.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China; Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
authors.forename: Peiping
authors.initials: P
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address: lsszqf@mail.sysu.edu.cn.
authors.forename: Qinfen
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000821:virology; D055032:Electron Microscope Tomography; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000502:physiology* / Q000648:ultrastructure*; D018411:Spodoptera / Q000821:virology; D014771:Virion / Q000502:physiology* / Q000648:ultrastructure*; D019065:Virus Assembly; D014779:Virus Replication
keywords: Baculovirus; Electron tomography; Envelopment process; Microvesicle formation; Occlusion-derived virion