title: Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins†Electronic supplementary information (ESI) available. See DOI: 10.1039/c4ra01027bClick here for additional data file.
abstract: We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins ofE. coli.
authors.affiliation: Institute of Physical & Theoretical Chemistry , Goethe-University Frankfurt , Max-von-Laue-Str. 7 , 60438 Frankfurt/Main , Germany . Email: heilemann@chemie.uni-frankfurt.de.
authors.forename: Anika
authors.initials: A
authors.lastname: Raulf
authors.affiliation: Institute of Physical & Theoretical Chemistry , Goethe-University Frankfurt , Max-von-Laue-Str. 7 , 60438 Frankfurt/Main , Germany . Email: heilemann@chemie.uni-frankfurt.de.
authors.forename: Christoph K
authors.initials: CK
authors.lastname: Spahn
authors.affiliation: Institute of Physical & Theoretical Chemistry , Goethe-University Frankfurt , Max-von-Laue-Str. 7 , 60438 Frankfurt/Main , Germany . Email: heilemann@chemie.uni-frankfurt.de.
authors.forename: Patrick J M
authors.initials: PJ
authors.lastname: Zessin
authors.affiliation: UCL Medical School , Gower St. , London , UK.
authors.forename: Kieran
authors.initials: K
authors.lastname: Finan
authors.affiliation: Institute for Organic Chemistry and Chemical Biology , Goethe-University Frankfurt , Max-von-Laue-Str. 9 , 60438 Frankfurt/Main , Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Bernhardt
authors.affiliation: Institute for Organic Chemistry and Chemical Biology , Goethe-University Frankfurt , Max-von-Laue-Str. 9 , 60438 Frankfurt/Main , Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Heckel
authors.affiliation: Institute of Physical & Theoretical Chemistry , Goethe-University Frankfurt , Max-von-Laue-Str. 7 , 60438 Frankfurt/Main , Germany . Email: heilemann@chemie.uni-frankfurt.de.
authors.forename: Mike
authors.initials: M
authors.lastname: Heilemann