title: Ultra-low temperature conservation of Brazilian pine embryogenic cultures.
abstract: This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Grasiela
authors.initials: G
authors.lastname: Demarchi
authors.affiliation: Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa, Campus São Gabriel, São Gabriel, RS, Brasil.
authors.forename: Valdir M
authors.initials: VM
authors.lastname: Stefenon
authors.affiliation: Laboratório de Fisiologia Vegetal, Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Neusa
authors.initials: N
authors.lastname: Steiner
authors.affiliation: Laboratório de Camarões Marinhos, Departamento de Aquicultura, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Felipe N
authors.initials: FN
authors.lastname: Vieira
authors.affiliation: Universidade Federal de Santa Catarina, Campus Curitibanos, Curitibanos, SC, Brasil.
authors.forename: Lirio L
authors.initials: LL
authors.lastname: Dal Vesco
authors.affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.
authors.forename: Miguel P
authors.initials: MP
authors.lastname: Guerra
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D058439:Plant Somatic Embryogenesis Techniques; D064028:Tracheophyta / Q000196:embryology*