title: DNA damage stress induces the expression of ribosomal protein S27a gene in a p53-dependent manner.
abstract: The small ribosomal protein RPS27a is known to play a role in the activation of cellular checkpoints via p53 which links ribosome biogenesis to cell cycle progression. Here, we show that RPS27a gene is a direct transcriptional target of p53 and is overexpressed in response to DNA damage. Elevated RPS27a level was associated with increased expression of p53 and its target p21(Waf1) gene. The RPS27a activity was specifically inhibited in the presence of a dominant negative mutant of p53. Down-regulation of ectopically expressed RPS27a by RNA interference blocked the activation of p21(waf1) in response to DNA damage. Thus, RPS27a appears to be a novel stress sensor in the cell which amplifies p53 response to arrest cell cycle.
authors.affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Nagisa
authors.initials: N
authors.lastname: Nosrati
authors.affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.
authors.forename: Neetu Rohit
authors.initials: NR
authors.lastname: Kapoor
authors.affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India. Electronic address: vijay@icgeb.res.in.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
mesh_terms: D059447:Cell Cycle Checkpoints; D002461:Cell Line, Transformed; D004249:DNA Damage; D005786:Gene Expression Regulation; D006801:Humans; D009154:Mutation; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012269:Ribosomal Proteins; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C096227:ribosomal protein S27a
keywords: DNA damage stress; RNA interference; RPS27a; Ribosomal RNA; p21(Waf1); p53