title: Two nearly complete mitogenomes of wheat stem borers, Cephus pygmeus (L.) and Cephus sareptanus Dovnar-Zapolskij (Hymenoptera: Cephidae): an unusual elongation of rrnS gene.
abstract: Two nearly complete mitochondrial genomes (mitogenomes) of wheat stem borers, Cephus pygmeus and Cephus sareptanus (Hymenoptera: Cephidae), were sequenced, characterised and compared with the previously known mitogenome of Cephus cinctus. The gene orders are mostly conserved, except for translocation of trnM and swapped position of trnI and trnQ. An A+T bias was found, but a deviation from strand asymmetry was also detected on the J strand. All protein coding genes (PCGs) are initiated by ATN codons, except for nad1, nad2 and atp8, and all are terminated with TAA, TA- or T- as a stop codon. The predicted secondary structures of rrnS and rrnL genes are mostly consistent with reported hymenopteran species. However, an unusual elongation in rrnS, not know elsewhere in the order, was discovered in Cephus species. Three autonomous sequences detected in domains I and II are mainly responsible for the length expansions.
authors.affiliation: Cumhuriyet University, Department of Molecular Biology and Genetics, Faculty of Science, Cumhuriyet University, Sivas 58140, Turkey. Electronic address: ekorkmaz@cumhuriyet.edu.tr.
authors.forename: Ertan Mahir
authors.initials: EM
authors.lastname: Korkmaz
authors.affiliation: Cumhuriyet University, Department of Molecular Biology and Genetics, Faculty of Science, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Özgül
authors.initials: Ö
authors.lastname: Doğan
authors.affiliation: Cumhuriyet University, Department of Molecular Biology and Genetics, Faculty of Science, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Mahir
authors.initials: M
authors.lastname: Budak
authors.affiliation: Cumhuriyet University, Department of Biology, Faculty of Science, Cumhuriyet University, Sivas 58140, Turkey.
authors.forename: Hasan Hüseyin
authors.initials: HH
authors.lastname: Başıbüyük
mesh_terms: D000818:Animals; D001483:Base Sequence; D018388:Codon, Terminator / Q000737:chemistry / Q000235:genetics; D042622:DNA Repeat Expansion; D017344:Genes, Insect; D050259:Genes, Mitochondrial; D049750:Genome, Insect / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D006927:Hymenoptera / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014908:Triticum / Q000469:parasitology*
chemical_list: D018388:Codon, Terminator
keywords: Mitochondrial genome; Sawflies; Secondary structure; Strand asymmetry; Symphyta; rRNAs