title: Monitoring RNA unwinding by the transcription termination factor Rho from Mycobacterium tuberculosis.
abstract: Transcription termination factor Rho is a ring-shaped, homo-hexamieric RNA translocase that dissociates transcription elongation complexes and transcriptional RNA-DNA duplexes (R-loops) in bacteria. The molecular mechanisms underlying these biological functions have been essentially studied with Rho enzymes from Escherichia coli or close Gram-negative relatives. However, phylo-divergent Rho factors may have distinct properties. Here, we describe methods for the preparation and in vitro characterization (ATPase and helicase activities) of the Rho factor from Mycobacterium tuberculosis, a specimen with uncharacteristic molecular and enzymatic features. These methods set the stage for future studies aimed at better defining the diversity of enzymatic properties of Rho across the bacterial kingdom.
authors.affiliation: Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Rue Charles Sadron, 45071, Orléans, cedex 2, France.
authors.forename: François
authors.initials: F
authors.lastname: D'Heygère
authors.forename: Annie
authors.initials: A
authors.lastname: Schwartz
authors.forename: Franck
authors.initials: F
authors.lastname: Coste
authors.forename: Bertrand
authors.initials: B
authors.lastname: Castaing
authors.forename: Marc
authors.initials: M
authors.lastname: Boudvillain
mesh_terms: D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D020365:RNA Helicases / Q000378:metabolism; D012234:Rho Factor / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000378:metabolism
chemical_list: D012234:Rho Factor; D017361:Viral Nonstructural Proteins; D012313:RNA; D020365:RNA Helicases