title: Reference region modeling approaches for amphetamine challenge studies with [11C]FLB 457 and PET.
abstract: Detecting fluctuations in synaptic dopamine levels in extrastriatal brain regions with [(11)C]FLB 457 and positron emission tomography (PET) is a valuable tool for studying dopaminergic dysfunction in psychiatric disorders. The evaluation of reference region modeling approaches would eliminate the need to obtain arterial input function data. Our goal was to explore the use of reference region models to estimate amphetamine-induced changes in [(11)C]FLB 457 dopamine D2/D3 binding. Six healthy tobacco smokers were imaged with [(11)C]FLB 457 at baseline and at 3 hours after amphetamine (0.4 to 0.5 mg/kg, per os) administration. Simplified reference tissue models, SRTM and SRTM2, were evaluated against the 2-tissue compartmental model (2TC) to estimate [(11)C]FLB 457 binding in extrastriatal regions of interest (ROIs), using the cerebellum as a reference region. No changes in distribution volume were observed in the cerebellum between scan conditions. SRTM and SRTM2 underestimated binding, compared with 2TC, in ROIs by 26% and 9%, respectively, with consistent bias between the baseline and postamphetamine scans. Postamphetamine, [(11)C]FLB 457 binding significantly decreased across several brain regions as measured with SRTM and SRTM2; no significant change was detected with 2TC. These data support the sensitivity of [(11)C]FLB 457 for measuring amphetamine-induced dopamine release in extrastriatal regions with SRTM and SRTM2.
authors.affiliation: 1] Department of Psychiatry, Yale University, New Haven, Connecticut, USA [2] Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Sandiego
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Jean-Dominique
authors.initials: JD
authors.lastname: Gallezot
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Keunpoong
authors.initials: K
authors.lastname: Lim
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Ropchan
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Shu-fei
authors.initials: SF
authors.lastname: Lin
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.affiliation: Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Evan D
authors.initials: ED
authors.lastname: Morris
authors.affiliation: 1] Department of Psychiatry, Yale University, New Haven, Connecticut, USA [2] Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
authors.forename: Kelly P
authors.initials: KP
authors.lastname: Cosgrove
mesh_terms: D000328:Adult; D000661:Amphetamine / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D015259:Dopamine Agents / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D049268:Positron-Emission Tomography; D011759:Pyrrolidines / Q000032:analysis / Q000378:metabolism*; D017448:Receptors, Dopamine D2 / Q000378:metabolism*; D050637:Receptors, Dopamine D3 / Q000378:metabolism*; D012457:Salicylamides / Q000032:analysis / Q000378:metabolism*; D012907:Smoking / Q000378:metabolism
chemical_list: D015259:Dopamine Agents; D011759:Pyrrolidines; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3; D012457:Salicylamides; C065065:FLB 457; D000661:Amphetamine