title: Dietary ractopamine influences sarcoplasmic proteome profile of pork Longissimus thoracis.
abstract: Dietary ractopamine improves pork leanness, whereas its effect on sarcoplasmic proteome has not been characterized. Therefore, the influence of ractopamine on sarcoplasmic proteome of post-mortem pork Longissimus thoracis muscle was examined. Longissimus thoracis samples were collected from carcasses (24 h post-mortem) of purebred Berkshire barrows (n=9) managed in mixed-sex pens and fed finishing diets containing ractopamine (RAC; 7.4 mg/kg for 14 days followed by 10.0 mg/kg for 14 days) or without ractopamine for 28 days (CON). Sarcoplasmic proteome was analyzed using two-dimensional electrophoresis and mass spectrometry. Nine protein spots were differentially abundant between RAC and CON groups. Glyceraldehyde-3-phosphate dehydrogenase and phosphoglucomutase-1 were over-abundant in CON, whereas serum albumin, carbonic anhydrase 3, L-lactate dehydrogenase A chain, fructose-bisphosphate aldolase A, and myosin light chain 1/3 were over-abundant in RAC. These results suggest that ractopamine influences the abundance of enzymes involved in glycolytic metabolism, and the differential abundance of glycolytic enzymes could potentially influence the conversion of muscle to meat.
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA; Departamento de Tecnologia de Alimentos, Faculdade de Veterinaria, Universidade Federal Fluminense, Niteroi, 24230-340 Rio de Janeiro, Brazil.
authors.forename: Bruno R C
authors.initials: BR
authors.lastname: Costa-Lima
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA. Electronic address: spsuma2@uky.edu.
authors.forename: Surendranath P
authors.initials: SP
authors.lastname: Suman
authors.affiliation: Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA.
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
authors.affiliation: Proteomics Core Facility, University of Kentucky, Lexington, KY 40506, USA.
authors.forename: Carol M
authors.initials: CM
authors.lastname: Beach
authors.affiliation: Departamento de Tecnologia de Alimentos, Faculdade de Veterinaria, Universidade Federal Fluminense, Niteroi, 24230-340 Rio de Janeiro, Brazil.
authors.forename: Teofilo J P
authors.initials: TJ
authors.lastname: Silva
authors.affiliation: Centro de Tecnologia de Carnes, Instituto de Tecnologia de Alimentos, Campinas, 13070-178 Sao Paulo, Brazil.
authors.forename: Expedito T F
authors.initials: ET
authors.lastname: Silveira
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Benjamin M
authors.initials: BM
authors.lastname: Bohrer
authors.affiliation: Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Dustin D
authors.initials: DD
authors.lastname: Boler
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001947:Breeding; D004032:Diet; D006019:Glycolysis / Q000187:drug effects*; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D018994:Myosin Light Chains / Q000378:metabolism; D064170:Paraspinal Muscles / Q000201:enzymology / Q000378:metabolism*; D010627:Phenethylamines / Q000494:pharmacology*; D011180:Postmortem Changes; D020543:Proteome / Q000378:metabolism*; D000069466:Red Meat / Q000032:analysis*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D006133:Growth Substances; D009124:Muscle Proteins; D018994:Myosin Light Chains; D010627:Phenethylamines; D020543:Proteome; C060897:ractopamine
keywords: Glycolysis; Longissimus thoracis; Ractopamine; Sarcoplasmic proteome