title: Planarian myosin essential light chain is involved in the formation of brain lateral branches during regeneration.
abstract: The myosin essential light chain (ELC) is a structure component of the actomyosin cross-bridge, however, the functions in the central nervous system (CNS) development and regeneration remain poorly understood. Planarian Dugesia japonica has revealed fundamental mechanisms and unique aspects of neuroscience and neuroregeneration. In this study, the cDNA DjElc, encoding a planarian essential light chain of myosin, was identified from the planarian Dugesia japonica cDNA library. It encodes a deduced protein with highly conserved functionally domains EF-Hand and Ca(2+) binding sites that shares significant similarity with other members of ELC. Whole mount in situ hybridization studies show that DjElc expressed in CNS during embryonic development and regeneration of adult planarians. Loss of function of DjElc by RNA interference during planarian regeneration inhibits brain lateral branches regeneration completely. In conclusion, these results demonstrated that DjElc is required for maintenance of neurons and neurite outgrowth, particularly for involving the brain later branch regeneration.
authors.affiliation: Laboratory of Developmental and Evolutionary Biology, School of Life Sciences, Shandong University of Technology, 12 Zhangzhou Road, Zibo, 255049, People's Republic of China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Yu
authors.forename: Xuhui
authors.initials: X
authors.lastname: Chen
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Yuan
authors.forename: Luming
authors.initials: L
authors.lastname: Zhou
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Pang
authors.forename: Bingyu
authors.initials: B
authors.lastname: Mao
authors.forename: Bosheng
authors.initials: B
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology / Q000378:metabolism* / Q000502:physiology; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D018994:Myosin Light Chains / Q000145:classification / Q000235:genetics*; D016501:Neurites / Q000378:metabolism / Q000502:physiology; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D010802:Phylogeny; D010932:Planarians / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D034622:RNA Interference; D012038:Regeneration / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015801:Helminth Proteins; D018994:Myosin Light Chains