title: Enrichment of aliphatic, alicyclic and aromatic acids by oil-degrading bacteria isolated from the rhizosphere of plants growing in oil-contaminated soil from Kazakhstan.
abstract: Three microbial strains were isolated from the rhizosphere of alfalfa (Medicago sativa), grass mixture (Festuca rubra, 75 %; Lolium perenne, 20 %; Poa pratensis, 10 %), and rape (Brassica napus) on the basis of their high capacity to use crude oil as the sole carbon and energy source. These isolates used an unusually wide spectrum of hydrocarbons as substrates (more than 80), including n-alkanes with chain lengths ranging from C12 to C32, monomethyl- and monoethyl-substituted alkanes (C12-C23), n-alkylcyclo alkanes with alkyl chain lengths from 4 to 18 carbon atoms, as well as substituted monoaromatic and diaromatic hydrocarbons. These three strains were identified as Gordonia rubripertincta and Rhodococcus sp. SBUG 1968. During their transformation of this wide range of hydrocarbon substrates, a very large number of aliphatic, alicyclic, and aromatic acids was detected, 44 of them were identified by GC/MS analyses, and 4 of them are described as metabolites for the first time. Inoculation of plant seeds with these highly potent bacteria had a beneficial effect on shoot and root development of plants which were grown on oil-contaminated sand.
authors.affiliation: Department of Applied Microbiology, Institute of Microbiology, University Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487, Greifswald, Germany, annett.mikolasch@uni-greifswald.de.
authors.forename: Annett
authors.initials: A
authors.lastname: Mikolasch
authors.forename: Anel
authors.initials: A
authors.lastname: Omirbekova
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Anne
authors.initials: A
authors.lastname: Reinhard
authors.forename: Halah
authors.initials: H
authors.lastname: Sheikhany
authors.forename: Ramza
authors.initials: R
authors.lastname: Berzhanova
authors.forename: Togzhan
authors.initials: T
authors.lastname: Mukasheva
authors.forename: Frieder
authors.initials: F
authors.lastname: Schauer
mesh_terms: D002244:Carbon / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism*; D004734:Energy Metabolism; D004787:Environmental Pollution; D008401:Gas Chromatography-Mass Spectrometry; D033601:Gordonia Bacterium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D007623:Kazakhstan; D010578:Petroleum / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology; D058441:Rhizosphere; D012240:Rhodococcus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*
chemical_list: D002264:Carboxylic Acids; D010578:Petroleum; D012989:Soil Pollutants; D002244:Carbon