title: Simple method for preparing glucose biosensor based on in-situ polypyrrole cross-linked chitosan/glucose oxidase/gold bionanocomposite film.
abstract: A film of chitosan-polypyrrole-gold nanoparticles was fabricated by in-situ chemical synthesis method and its application in glucose biosensor was investigated. The obtained biosensor exhibited a high and reproducible sensitivity of 0.58μA/mM, response time ~4s, linear dynamic range from 1 to 20mM, correlation coefficient of R(2)=0.9981, and limit of detection (LOD), based on S/N ratio (S/N=3) of 0.068mM. A value of 1.83mM for the apparent Michaelis-Menten constant was obtained. The resulting bio-nanocomposite provided a suitable environment for the enzyme to retain its bioactivity at considerably extreme conditions, and the decorated gold nanoparticles in the bio-nanocomposite offer good affinity to enzyme.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey. Electronic address: msenel@fatih.edu.tr.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Şenel
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis; D048271:Chitosan / Q000737:chemistry; D058266:Dielectric Spectroscopy; D004566:Electrodes; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011758:Pyrroles / Q000737:chemistry; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D001786:Blood Glucose; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006046:Gold; D048271:Chitosan; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Chitosan; Glucose oxidase; Immobilization; Pyrrole