title: Effects of low-doses of Bacillus spp. from permafrost on differentiation of bone marrow cells.
abstract: The effects of a new microorganism species (Bacillus spp., strain M3) isolated from permafrost specimens from Central Yakutia (Mamontova Mountain) on the bone marrow hemopoiesis were studied on laboratory mice. Analysis of the count and immunophenotype of bone marrow cells indicated that even in low doses (1000-5000 microbial cells) these microorganisms modulated hemopoiesis and lymphopoiesis activity. The percentage of early hemopoietic precursors (CD117(+)CD34(-)) increased, intensity of lymphocyte precursor proliferation and differentiation (CD25(+)CD44(-)) decreased, and the percentage of lymphocytes released from the bone marrow (CD25(+)CD44(+)) increased on day 21 after injection of the bacteria. These changes in activity of hemopoiesis were associated with changes in the level of regulatory T lymphocytes (reduced expression of TCRαβ) and were most likely compensatory. The possibility of modulating hemopoiesis activity in the bone marrow by low doses of one microorganism strain isolated from the permafrost could be useful for evaluating the effects of other low dose bacteria on the bone marrow hemopoiesis.
authors.affiliation: Tyumen Research Center, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia, lkalenova@mail.ru.
authors.forename: L F
authors.initials: LF
authors.lastname: Kalyonova
authors.forename: M A
authors.initials: MA
authors.lastname: Novikova
authors.forename: E G
authors.initials: EG
authors.lastname: Kostolomova
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D001407:Bacillus / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D008297:Male; D051379:Mice; D065946:Permafrost / Q000382:microbiology*; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism
chemical_list: D018952:Antigens, CD34; D019009:Proto-Oncogene Proteins c-kit