title: Using deep sequencing data for identification of editing sites in mature miRNAs.
abstract: Deep sequencing has many possible applications; one of them is the identification and quantification of RNA editing sites. The most common type of RNA editing is adenosine to inosine (A-to-I) editing. A prerequisite for this editing process is a double-stranded RNA (dsRNA) structure. Such dsRNAs are formed as part of the microRNA (miRNA) maturation process, and it is therefore expected that miRNAs are affected by A-to-I editing. Indeed, tens of editing sites were found in miRNAs, some of which change the miRNA binding specificity. Here, we describe a protocol for the identification of RNA editing sites in mature miRNAs using deep sequencing data.
authors.affiliation: Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, 69978, Israel.
authors.forename: Shahar
authors.initials: S
authors.lastname: Alon
authors.forename: Eli
authors.initials: E
authors.lastname: Eisenberg
mesh_terms: D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D035683:MicroRNAs / Q000235:genetics*; D017393:RNA Editing / Q000235:genetics*; D012330:RNA, Double-Stranded / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D012330:RNA, Double-Stranded