title: [Establishment of experimental abdominal aortic aneurysm model in rats].
abstract: OBJECTIVE
  To establish a new reliable experimental abdominal aortic aneurysm (AAA) rat model that simulates human aneurysms and has high survival rate.
  
  
  METHODS
  Twenty-four SD rats were randomly divided equally into two groups (male: female=1: 1). Intraluminal saline infusion was given to rats in group A. Rats in group B were given intraluminal elastase infusion and extraluminal calcium chloride application. The diameter of abdominal aorta was measured and eventually harvested at 4 weeks. Successful AAA formation was defined as a dilation ratio )50%. HE stain was applied to evaluate the histology changes of aorta.
  
  
  RESULTS
  Group A had a survival rate, technical success rate and AAA formation rate of 83. 3%, 91. 67%, and 0% respectively, compared with 91. 67%, 100%, and 90. 91% in group B. The AAA formation rate in group B was significantly higher than that in group A (P<0. 05). The dilation ratio during infusion was 50. 82% ±4. 11% in group A and 48. 19% ± 3. 89% in group B. The dilation ratio after infusion was 29. 55% ± 4. 69% in group A and 27. 89% + 4. 28% in group B. The difference of dilation ratio between the two groups was not significant. The post-surgery (28 d) dilation ratio was 27. 89% ± 4. 28%0 in group A and 88. 98% ± 42. 33% in group B (P< 0. 05). Fusiform aneurysms, local adhesion and calcification were found in group B. Rats in group B also had thickening intimal, degenerated matrix in media, loss of elastin fiber, apoptosis of vascular smooth muscle cells and transmural inflammation, none of which was observed in group A.
  
  
  CONCLUSIONS
  With improved operative procedure, intraluminal elastase infusion and extraluminal calcium chloride application can create reliable AAA rat model.
authors.affiliation: Department of Vascular Surgery,  West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xiong
authors.forename: Guo-iun
authors.initials: GI
authors.lastname: Zeng
authors.forename: Ji-chun
authors.initials: JC
authors.lastname: Zhao
authors.forename: Yu-kui
authors.initials: YK
authors.lastname: Ma
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhou-peng
authors.initials: ZP
authors.lastname: Wu
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology; D017544:Aortic Aneurysm, Abdominal; D017209:Apoptosis; D002122:Calcium Chloride; D004108:Dilatation, Pathologic; D004195:Disease Models, Animal; D005260:Female; D007249:Inflammation; D008297:Male; D010196:Pancreatic Elastase; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D010196:Pancreatic Elastase; D002122:Calcium Chloride