title: Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression.
abstract: OBJECTIVE
  To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10(-/-) mice.
  
  
  METHODS
  To synchronize colitis, 8- to 10-wk-old IL-10(-/-) mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10(-/-) mice and in Colo 205 cells.
  
  
  RESULTS
  Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1β, interferon (IFN)-γ and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the butein-treated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells.
  
  
  CONCLUSIONS
  Butein ameliorated colitis in IL-10(-/-) mice by regulating IL-6/STAT3 and MMP-9 activation.
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Sehe Dong
authors.initials: SD
authors.lastname: Lee
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Jung Wan
authors.initials: JW
authors.lastname: Choe
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Beom Jae
authors.initials: BJ
authors.lastname: Lee
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Myoung Hee
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Moon Kyung
authors.initials: MK
authors.lastname: Joo
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Jong Eun
authors.initials: JE
authors.lastname: Yeon
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Jong-Jae
authors.initials: JJ
authors.lastname: Park
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Jae Seon
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Sehe Dong Lee, Jung Wan Choe, Beom Jae Lee, Myoung Hee Kang, Moon Kyung Joo, Ji Hoon Kim, Jong Eun Yeon, Jong-Jae Park, Jae Seon Kim, Young-Tae Bak, Division of Gastroenterology, Department of Internal Medicine, Korea University Medical Center, Seoul 152-703, South Korea.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Bak
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D047188:Chalcones / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects* / Q000378:metabolism; D004195:Disease Models, Animal; D004789:Enzyme Activation; D006801:Humans; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics; D015850:Interleukin-6 / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D018345:Mice, Knockout; D010894:Piroxicam; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D047188:Chalcones; C556062:IL10 protein, mouse; D015850:Interleukin-6; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C494087:Stat3 protein, mouse; C577686:interleukin-6, mouse; D016753:Interleukin-10; D010894:Piroxicam; C040918:butein; D020780:Matrix Metalloproteinase 9; C501830:Mmp9 protein, mouse
keywords: Butein; Colitis; Inflammatory bowel disease; Interleukin-6/signal transducer and activator of transcription 3; Matrix metalloproteinase-9