title: From guide to target: molecular insights into eukaryotic RNA-interference machinery.
abstract: Since its relatively recent discovery, RNA interference (RNAi) has emerged as a potent, specific and ubiquitous means of gene regulation. Through a number of pathways that are conserved in eukaryotes from yeast to humans, small noncoding RNAs direct molecular machinery to silence gene expression. In this Review, we focus on mechanisms and structures that govern RNA silencing in higher organisms. In addition to highlighting recent advances, we discuss parallels and differences among RNAi pathways. Together, the studies reviewed herein reveal the versatility and programmability of RNA-induced silencing complexes and emphasize the importance of both upstream biogenesis and downstream silencing factors.
authors.affiliation: 1] W.M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. [2] Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
authors.forename: Jonathan J
authors.identifier: 0000000227433776
authors.initials: JJ
authors.lastname: Ipsaro
authors.affiliation: 1] W.M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA. [2] Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
authors.forename: Leemor
authors.initials: L
authors.lastname: Joshua-Tor
mesh_terms: D056890:Eukaryota / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism*
chemical_list: D022661:RNA, Untranslated