title: Effect of Amphotericin B Nanodisks onLeishmania majorInfected Mice.
abstract: OBJECTIVE
  To assess the efficacy of a novel formulation of the polyene antibiotic, amphotericin B (AMB), as therapy for cutaneous leishmaniasis in different mouse strains.
  
  
  METHODS
  (AMB), was formulated into water-soluble transport particles, termed nanodisks (ND). Balb/c and CH3mice infected withLeishmania majoron Day 0 were administered vehicle alone, empty ND or AMB-ND on Day 1 and day 7, via the tail vein. Mice were sacrificed 25 or 50 days post inoculation and tissue histology evaluated. Balb/c mice treated with vehicle or empty ND showed signs of severe infection while CH3mice had less inflammation and fewer parasites. AMB-ND treatment (2 mg/kg) had a marked therapeutic effect onL. majorinfected Balb/c mice and a discernable therapeutic benefit on CH3mice.
  
  
  CONCLUSIONS
  AMB-ND is efficacious in the treatment of cutaneous leishmaniasis in both susceptible and resistant mouse strains. It may be inferred that AMB-ND may be useful for prophylactic and/or treatment of early stageLeishmania spp.infection.
authors.affiliation: Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523-1619.
authors.forename: Pa
authors.initials: P
authors.lastname: Cole
authors.affiliation: Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523-1619.
authors.forename: Jv
authors.initials: J
authors.lastname: Bishop
authors.affiliation: Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland CA 94609, USA.
authors.forename: Ja
authors.initials: J
authors.lastname: Beckstead
authors.affiliation: Microbiology, Immunology, and Pathology Department, Colorado State University, Fort Collins, CO 80523-1619.
authors.forename: R
authors.initials: R
authors.lastname: Titus
authors.affiliation: Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland CA 94609, USA.
authors.forename: Ro
authors.initials: R
authors.lastname: Ryan