title: Nanogels based on alginic aldehyde and gelatin by inverse miniemulsion technique: synthesis and characterization.
abstract: Nanogels were developed from alginic aldehyde and gelatin by an inverse miniemulsion technique. Stable inverse miniemulsions were prepared by sonication of noncontinuous aqueous phase (mixture of alginic aldehyde and gelatin) in a continuous organic phase (Span 20 dissolved in cyclohexane). Cross-linking occurred between alginic aldehyde (AA) and gelatin (gel) in the presence of borax by Schiff's base reaction during the formation of inverse miniemulsion. The effects of surfactant (Span 20) concentration, volume of the aqueous phase and AA/gel weight ratio on the size of the alginic aldehyde-gelatin (AA-gel) nanoparticles were studied. Nanogels were characterized by DLS, FT-IR spectroscopy, TGA, SEM and TEM. DLS, TEM and SEM studies demonstrated nanosize and spherical morphology of the nanogels. Hemocompatibility and in vitro cytocompatibility analyses of the nanogels proved their nontoxicity. The results indicated the potential of the present nanogel system as a candidate for drug- and gene-delivery applications.
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology (IIST), Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: sarikapaithal@gmail.com.
authors.forename: P R
authors.initials: PR
authors.lastname: Sarika
authors.affiliation: Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, Kerala 695 012, India. Electronic address: anilkumarpr@sctimst.ac.in.
authors.forename: P R
authors.initials: PR
authors.lastname: Anil Kumar
authors.affiliation: Tissue Culture Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojappura, Thiruvananthapuram, Kerala 695 012, India. Electronic address: kdeeps3@gmail.com.
authors.forename: Deepa K
authors.initials: DK
authors.lastname: Raj
authors.affiliation: Department of Chemistry, Indian Institute of Space Science and Technology (IIST), Valiamala, Thiruvananthapuram, Kerala 695 547, India. Electronic address: nirmala@iist.ac.in.
authors.forename: Nirmala Rachel
authors.initials: NR
authors.lastname: James
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry*; D004655:Emulsions / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D061986:MCF-7 Cells; D008970:Molecular Weight; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000737:chemistry; D013818:Thermogravimetry
chemical_list: D000447:Aldehydes; D000464:Alginates; D004655:Emulsions; D000080385:Nanogels; D013501:Surface-Active Agents; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D005780:Gelatin; D011094:Polyethyleneimine; D007770:L-Lactate Dehydrogenase
keywords: Alginic aldehyde; Gelatin; Inverse miniemulsion; Nanogels; Schiff's base reaction.