title: Weak D Type 4.2.2 (DAR1.2) in an African child: Serology and molecular characterization.
abstract: The weak D phenotype is represented by a group of RHD genotypes that code for alterated RhD proteins associated with a reduced RhD expression on red blood cell. By routine serology, some partial D variants are likely to be missed. In this report we describe the case of a three-year-old Black African child with a "unclear" reaction with monoclonal anti-D. We analyzed the blood sample of the child with different methods to conclude that it is a case of DAR 1.2 (weak D 4.2.2) and that it must be transfused with D negative erithrocytes.
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Orlando
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy. Electronic address: rossana.putzulu@rm.unicatt.it.
authors.forename: Rossana
authors.initials: R
authors.lastname: Putzulu
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Massini
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Fernando
authors.initials: F
authors.lastname: Scavone
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Piccirillo
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Maresca
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Gina
authors.initials: G
authors.lastname: Zini
authors.affiliation: Transfusion Medicine Department, Università Cattolica Sacro Cuore, L.go A.Gemelli, Rome 8- 00168, Italy.
authors.forename: Luciana
authors.initials: L
authors.lastname: Teofili
mesh_terms: D000349:Africa; D000483:Alleles; D044383:Black People; D001788:Blood Grouping and Crossmatching / Q000379:methods*; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D004912:Erythrocytes / Q000166:cytology; D005091:Exons; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012204:Rh-Hr Blood-Group System / Q000235:genetics*; D018029:Rho(D) Immune Globulin / Q000097:blood*; D012699:Serology / Q000379:methods*
chemical_list: C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin
keywords: BeadChip; D-Partial; D-Weak