title: Effect of magnetized extender on sperm membrane integrity and development of oocytes in vitro fertilized with liquid storage boar semen.
abstract: The objective of this study was to evaluate the effect of a magnetized extender on sperm membrane damage and development of oocytes in vitro fertilized with liquid storage boar semen. Before semen dilution, extender was flowed through a neodymium magnet (0, 2000, 4000 and 6000G) for 5min and collected semen was preserved for 168h at 18°C. In results, plasma membrane integrity with live sperm was significantly higher in semen treated with extenders magnetized at 4000G than sperm treated with extenders magnetized at 0G during semen preservation for 120-168h (p<0.05). In addition, acrosomal membrane damage was significantly lower in semen treated with extenders magnetized at 4000 and 6000G compared to 0 and 2000G during semen preservation for 168h (p<0.05). And mitochondrial membrane damage with all sperm was significantly lower in semen treated with extenders magnetized at 2000G than other groups during semen preservation for 168h. The ability of semen to achieve successful in vitro fertilization was also not significantly different among the groups during preservation. However, when the semen was preserved for 168h, the blastocyst formation rates were significantly higher at 6000G compared to 0 and 2000G (p<0.05). In conclusion, these results suggest that highly magnetized semen extender could protect the sperm membrane from damage, and improve the ability of rates of in vitro blastocyst development and magnetized semen diluter is beneficial for long liquid preservation of boar semen.
authors.affiliation: College of Animal Life Science, Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: College of Animal Life Science, Division of Applied Animal Science, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: parkck@kangwon.ac.kr.
authors.forename: Choon-Keun
authors.initials: CK
authors.lastname: Park
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D008280:Magnetics; D008297:Male; D009865:Oocytes / Q000502:physiology*; D012662:Semen Preservation / Q000379:methods*; D013094:Spermatozoa / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D013552:Swine
keywords: Boar sperm; Liquid preservation; Magnetized water; Membrane damage