title: In silico structural analysis of group 3, 6 and 9 allergens from Dermatophagoides farinae.
abstract: Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) are the predominant source of dust mite allergens, which provoke allergic diseases, such as rhinitis, asthma and eczema. Of the 30 allergen groups produced by D. farinae, the Der f 3, Der f 6 and Der f 9 allergens are all trypsin‑associated proteins, however little else is currently known about them. The present study used in silico tools to compare the amino acid sequences, and predict the secondary and tertiary structures of Der f 3, Der f 6 and Der f 9 allergens. Protein sequence alignment detected ~46% identity between Der f 3, Der f 6 and Der f 9. Furthermore, each protein was shown to contain three active sites and two highly conserved trypsin functional domains. Predictions of the secondary and tertiary structure identified α‑helices, β‑sheets and random coils. The active sites of the three proteins appeared to fold onto each other in a three‑dimensional model, constituting the active site of the enzyme. Epitope analysis demonstrated that Der f 3, Der f 6 and Der f 9 have 4‑5 potential epitopes located in random coils, and the epitope sequences of Der f 3, Der f 6 and Der f 9 were shown to overlap in two domains (at amino acids 83‑87 and 179‑180); however the residues in these two domains were not identical. The present study aimed to conduct a biochemical and genetic analysis of these three allergens, and to potentially contribute to the development of vaccines for allergen‑specific immunotherapy.
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Feixiang
authors.initials: F
authors.lastname: Teng
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Bian
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Juansong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Cunbao
authors.initials: C
authors.lastname: Ling
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Yungang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Yancheng Institute of Health Sciences, Yancheng, Jiangsu 224006, P.R. China.
authors.forename: Yubao
authors.initials: Y
authors.lastname: Cui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000737:chemistry* / Q000276:immunology*; D020134:Catalytic Domain; D040001:Dermatophagoides farinae / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016415:Sequence Alignment; D013329:Structure-Activity Relationship
chemical_list: D039741:Antigens, Dermatophagoides; D018985:Epitopes, B-Lymphocyte