title: Novel MesoPorous BioGlass/silk scaffold containing adPDGF-B and adBMP7 for the repair of periodontal defects in beagle dogs.
abstract: OBJECTIVE
  The local delivery of growth factors via gene therapy has gained tremendous awareness in recent years due to their sustained growth factor delivery to target tissues. The aim of this study was to fabricate and investigate a scaffold able to release growth factors via gene therapy for the repair of periodontal tissues.
  
  
  METHODS
  Novel mesoporous bioglass (MBG)/silk fibrin scaffold combined with BMP7 and/or PDGF-B adenovirus was fabricated and tested in vitro for cell migration, proliferation and differentiation. Furthermore, acute-type buccal dehiscence periodontal defects (mesiodistal width × depth: 5 × 5 mm) were created on the buccal portion of the maxillary premolars in five normal male beagle dogs (12 months old, 15.0 ± 2.0 kg) and histologically examined for periodontal regeneration following implantation of the following five groups: (1) no scaffold, (2) MBG/silk scaffold alone, (3) scaffold + adPDGF-B, (4) scaffold + adBMP7, (5) scaffold + adPDGF-b + adBMP7.
  
  
  RESULTS
  In vitro findings demonstrated that adPDGF-B was able to rapidly recruit periodontal ligament (PDL) cells over sixfold more effectively than adBMP7, whereas adBMP7 was more able to induce osteoblast differentiation of PDL cells. In vivo findings demonstrate that scaffolds loaded with adPDGF-B were able to partially regenerate the periodontal ligament while adBMP7 scaffolds primarily improved new bone formation. The combination of both adPDGF-B and adBMP7 synergistically promoted periodontal regeneration by allowing up to two times greater regeneration of the periodontal ligament, alveolar bone and cementum when compared to each adenovirus used alone.
  
  
  CONCLUSIONS
  Although both PDGF-B and BMP7 are individually capable of promoting periodontal regeneration to some degree, their combination synergistically promotes wound healing in acute-type buccal dehiscence periodontal defects when delivered simultaneously. This study demonstrates the promise for successful delivery of low-cost, effective growth factor delivery via gene therapy for the treatment of periodontal defects.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral Implantology, School of Stomatology, Wuhan University, Wuhan, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Miron
authors.forename: Sue
authors.initials: S
authors.lastname: Li
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.forename: Anton
authors.initials: A
authors.lastname: Sculean
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D016301:Alveolar Bone Loss / Q000601:surgery*; D000818:Animals; D000077214:Becaplermin; D055419:Bone Morphogenetic Protein 7 / Q000627:therapeutic use*; D018786:Bone Substitutes / Q000737:chemistry*; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D025021:Cementogenesis / Q000502:physiology; D002516:Ceramics / Q000737:chemistry*; D004285:Dogs; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation / Q000379:methods; D008297:Male; D008439:Maxillary Diseases / Q000601:surgery; D010006:Osteoblasts / Q000502:physiology; D010012:Osteogenesis / Q000502:physiology; D010513:Periodontal Ligament / Q000166:cytology; D016062:Porosity; D020574:Proto-Oncogene Proteins c-sis / Q000627:therapeutic use*; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D055419:Bone Morphogenetic Protein 7; D018786:Bone Substitutes; D020574:Proto-Oncogene Proteins c-sis; D047011:Silk; D000077214:Becaplermin
keywords: gene therapy; growth factors; intrabony defect; osteoinduction; periodontal defect