title: Effect of basic and acidic additives on the separation of some basic drug enantiomers on polysaccharide-based chiral columns with acetonitrile as mobile phase.
abstract: The separation of enantiomers of 16 basic drugs was studied using polysaccharide-based chiral selectors and acetonitrile as mobile phase with emphasis on the role of basic and acidic additives on the separation and elution order of enantiomers. Out of the studied chiral selectors, amylose phenylcarbamate-based ones more often showed a chiral recognition ability compared to cellulose phenylcarbamate derivatives. An interesting effect was observed with formic acid as additive on enantiomer resolution and enantiomer elution order for some basic drugs. Thus, for instance, the enantioseparation of several β-blockers (atenolol, sotalol, toliprolol) improved not only by the addition of a more conventional basic additive to the mobile phase, but also by the addition of an acidic additive. Moreover, an opposite elution order of enantiomers was observed depending on the nature of the additive (basic or acidic) in the mobile phase.
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia.
authors.forename: Khatuna
authors.initials: K
authors.lastname: Gogaladze
authors.forename: Lali
authors.initials: L
authors.lastname: Chankvetadze
authors.forename: Maia
authors.initials: M
authors.lastname: Tsintsadze
authors.forename: Tivadar
authors.initials: T
authors.lastname: Farkas
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
mesh_terms: D000097:Acetonitriles; D000688:Amylose / Q000737:chemistry*; D002482:Cellulose / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013237:Stereoisomerism
chemical_list: D000097:Acetonitriles; D002482:Cellulose; D000688:Amylose; C032159:acetonitrile
keywords: basic chiral drugs; effect of minor additives; enantiomer elution order; polysaccharide-based chiral selectors; separation of enantiomers