title: IgE Abs to Der p 1 and Der p 2 as diagnostic markers of house dust mite allergy as defined by a bronchoprovocation test.
abstract: BACKGROUND
  Limited information is available regarding the clinical usefulness of measuring the levels of IgE to allergen components from house dust mites (HDMs) in the diagnosis of genuine HDM allergy.
  
  
  METHODS
  To evaluate the diagnostic usefulness of measuring levels of serum IgE antibodies (Abs) to allergen components from Dermatophagoides pteronyssinus (DP) as a predictor of immediate asthmatic response (IAR) to bronchoprovocation, we studied 55 DP-sensitized asthmatic patients who underwent a bronchoprovocation test using crude DP extract. The levels of IgE Abs to crude DP, nDer p 1, rDer p 2, and rDer p 10 in patients who showed IAR (n = 41) were compared with those in patients who showed no IAR (n = 14).
  
  
  RESULTS
  While the frequencies of positivity for IgE Abs to nDer p 1 and rDer p 2 among the entire study population were 89 and 86%, respectively, all patients with IAR tested positive for both of them with high IgE concentrations. The areas under the receiver operating characteristic curves for IgE to nDer p 1 and rDer p 2 as predictors of IAR were 0.913 and 0.906, respectively. The specificity of IgE to nDer p 1 and rDer p 2 was higher than IgE to crude DP even at low cut-off points.
  
  
  CONCLUSIONS
  IgE to nDer p 1 and/or rDer p 2 was highly predictive of allergen-induced IAR. These findings validate the clinical usefulness of measuring the levels of IgE to nDer p 1 and rDer p 2 as a diagnostic tool for genuine HDM allergy.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Minami
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan. Electronic address: y-fukutomi@sagamihara-hosp.gr.jp.
authors.forename: Yuma
authors.initials: Y
authors.lastname: Fukutomi
authors.affiliation: Thermo Fisher Scientific, Uppsala, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Lidholm
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasueda
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Saito
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sekiya
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuburai
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maeda
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Masami
authors.initials: M
authors.lastname: Taniguchi
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Akiyama
mesh_terms: D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides / Q000276:immunology*; D060829:Arthropod Proteins / Q000276:immunology*; D015415:Biomarkers; D001985:Bronchial Provocation Tests; D003546:Cysteine Endopeptidases / Q000276:immunology*; D040002:Dermatophagoides pteronyssinus / Q000276:immunology*; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012372:ROC Curve
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; D015415:Biomarkers; C451163:Dermatophagoides pteronyssinus antigen p 2; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1
keywords: Bronchoprovocation; Component-resolved diagnostics; Dermatophagoides pteronyssinus; House dust mite; Molecular-based allergy diagnostics