title: Evaluation of oxidative DNA damage and antioxidant defense in patients with nasal polyps.
abstract: OBJECTIVE
  The presence of inflammatory cells indicates the development of epithelial cell injury in nasal polyposis (NP) and the potential for production of high levels of reactive oxygen and nitrogen species. The aim of our study was to clarify the role of oxidative stress and antioxidant status in the deterioration accompanying NP.
  
  
  METHODS
  Twenty patients (11 men) aged 47.2 ± 17.0 years with nasal polyps were included in the study. Twenty healthy subjects (7 men) aged 48.2 ± 15.3 years formed the control group. The erythrocyte activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and plasma nitric oxide (NO) concentrations were measured. An alkaline comet assay was used to determine the extent of blood lymphocyte DNA damage of oxidized purines as glicosylo-formamidoglicosylase (Fpg) sites, and oxidized pyrimidines as endonuclease III (Nth) sites.
  
  
  RESULTS
  A significant increase of NO (P < 0.05) and non-significant decreases of SOD (P > 0.05), CAT (P > 0.05), and GPx (P > 0.05) were seen in NP patients compared to healthy controls. The level of blood lymphocyte oxidative DNA damage in NP patients was significantly higher compared to the control group (P = 0.01).
  
  
  CONCLUSIONS
  The blood lymphocyte DNA damage level increased in patients with NP. Elevated DNA damage may be related to overproduction of reactive oxygen and nitrogen species and/or decreased antioxidant protection.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Mrowicka
authors.forename: Hanna
authors.initials: H
authors.lastname: Zielinska-Blizniewska
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Milonski
authors.forename: Jurek
authors.initials: J
authors.lastname: Olszewski
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Majsterek
mesh_terms: D000328:Adult; D002374:Catalase / Q000097:blood; D002908:Chronic Disease; D020552:Comet Assay; D004249:DNA Damage; D043382:DNA-Formamidopyrimidine Glycosylase / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007249:Inflammation; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000097:blood* / Q000235:genetics; D009569:Nitric Oxide / Q000097:blood; D010084:Oxidation-Reduction; D011687:Purines / Q000097:blood; D011743:Pyrimidines / Q000097:blood; D026361:Reactive Nitrogen Species / Q000097:blood; D017382:Reactive Oxygen Species / Q000097:blood; D012852:Sinusitis / Q000097:blood; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D011687:Purines; D011743:Pyrimidines; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D043382:DNA-Formamidopyrimidine Glycosylase
keywords: Antioxidant enzymes; Comet assay; DNA damage; Nasal polyps; Oxidative stress