title: Nitric oxide participates in the regulation of the ascorbate-glutathione cycle by exogenous jasmonic acid in the leaves of wheat seedlings under drought stress.
abstract: In this paper, we investigated whether nitric oxide (NO) participated in the regulation of the ascorbate-glutathione (AsA-GSH) cycle by exogenous jasmonic acid (JA) in the leaves of wheat seedlings under drought stress. The findings of our study showed that drought stress significantly enhanced the AsA-GSH cycle by upregulating the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR). Drought stress also markedly increased electrolyte leakage (EL), malondialdehyde (MDA) content, NO content, and significantly reduced the ratios of reduced ascorbate/dehydroascorbic acid (AsA/DHA) and reduced glutathione/oxidized glutathione (GSH/GSSG) compared with control. Exogenous JA significantly increased the above indicators, compared with drought stress alone. All these effects of JA were inhibited by pretreatment with NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). Meanwhile, exogenous JA markedly decreased MDA content and electrolyte leakage of wheat leaves under drought stress. Pretreatment with cPTIO reversed the above effects of exogenous JA. Our findings indicated that NO induced by exogenous JA upregulated the activity of the AsA-GSH cycle and had important role in drought tolerance.
authors.affiliation: Henan Institute of Science and Technology, Xinxiang, 453003, China, shchjuan1978@aliyun.com.
authors.forename: Changjuan
authors.initials: C
authors.lastname: Shan
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Mingjiu
authors.initials: M
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000502:physiology; D003517:Cyclopentanes / Q000494:pharmacology; D003681:Dehydration; D055864:Droughts; D005978:Glutathione / Q000502:physiology; D015227:Lipid Peroxidation; D053858:Metabolic Networks and Pathways; D009569:Nitric Oxide / Q000502:physiology*; D018384:Oxidative Stress; D054883:Oxylipins / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism*; D017382:Reactive Oxygen Species; D036226:Seedlings / Q000378:metabolism*; D015398:Signal Transduction; D014908:Triticum / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003517:Cyclopentanes; D054883:Oxylipins; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C011006:jasmonic acid; D005978:Glutathione; D001205:Ascorbic Acid