title: 3D-printed microfluidic device for the detection of pathogenic bacteria using size-based separation in helical channel with trapezoid cross-section.
abstract: A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used to capture E. coli (EC) bacteria in milk, and the free MNCs and MNC-EC complexes were separated from the milk using a permanent magnet. The free MNCs and MNC-EC complexes were dispersed in a buffer solution, then the solution was injected into a helical microchannel device with or without a sheath flow. The MNC-EC complexes were separated from the free MNCs via the Dean drag force and lift force, and the separation was facilitated in the presence of a sheath flow. The concentration of the E. coli bacteria was determined using a light absorption spectrometer, and the limit of detection was found to be 10 cfu/mL in buffer solution and 100 cfu/mL in milk.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Wonjae
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Donghoon
authors.initials: D
authors.lastname: Kwon
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Choi
authors.affiliation: School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea.
authors.forename: Gyoo Yeol
authors.initials: GY
authors.lastname: Jung
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Jeon
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004867:Equipment Design; D004926:Escherichia coli / Q000302:isolation & purification* / Q000648:ultrastructure; D049449:Luminescence; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010316:Particle Size; D066330:Printing, Three-Dimensional / Q000295:instrumentation*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D058185:Magnetite Nanoparticles; D000255:Adenosine Triphosphate