title: Next-generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp).
abstract: Vesicular stomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
authors.forename: Fredrik
authors.initials: F
authors.lastname: Barrenas
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Green
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Thomas
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
authors.forename: G Lynn
authors.initials: GL
authors.lastname: Law
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
authors.forename: Sean C
authors.initials: SC
authors.lastname: Proll
authors.affiliation: Vaccine and Gene Therapy Institute, Beaverton, Oregon, USA.
authors.forename: Flora
authors.initials: F
authors.lastname: Engelmann
authors.affiliation: Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, Oregon, USA.
authors.forename: Ilhem
authors.initials: I
authors.lastname: Messaoudi
authors.affiliation: Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marzi
authors.affiliation: Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.affiliation: Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA honey@uw.edu.
authors.forename: Michael G
authors.initials: MG
authors.lastname: Katze
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D015023:Democratic Republic of the Congo; D046129:Ebola Vaccines / Q000276:immunology*; D029043:Ebolavirus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D015870:Gene Expression; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology*; D059014:High-Throughput Nucleotide Sequencing; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008252:Macaca fascicularis; D017423:Sequence Analysis, RNA; D013997:Time Factors; D059467:Transcriptome; D014614:Vaccines, Synthetic / Q000276:immunology; D018116:Vesiculovirus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D046129:Ebola Vaccines; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C113189:envelope glycoprotein, Ebola virus