title: Exercise training is more effective than resveratrol supplementation on alleviation of inflammation in peritoneal macrophages of high fat diet mice.
abstract: OBJECTIVE
  This study investigated the effect of exercise training and resveratrol supplementation with low fat diet on proinflammatory profiles by Lipopolysaccharide (LPS)-stimulation in peritoneal macrophage of high fat diet mice.
  
  
  METHODS
  To accomplish the purpose of this study, C57BL/6 male mice were fed high fat diet (45% fat diet) for 8 weeks. Then these mice were divided into 3 groups; HLC (high fat diet and low fat diet for 8 weeks as the control, n=10), HLR (high fat diet and low fat diet for 8 weeks with resveratrol supplementation, n=10). HLE (high fat diet and low fat diet for 8 weeks with moderate exercise training, n=10). Resveratrol (10 mg/kg) was administrated once a day, 5 days/week for 8 weeks. Exercise training was performed for 8 weeks on a treadmill running for 30-60 min/day at 10-22 m/min, 0% grade, 5 days/week. After exercise training, all the peritoneal macrophage was collected and LPS (0, 0.5, 1.0 μg/ml) were used to stimulate the cells. Then peritoneal macrophage TNF-α, IL-6, MCP-1, IL12p70, IFN-γ, IL-10 were measured by BD cytometric bead array mouse inflammation kit.
  
  
  RESULTS
  As a result, body weight and total cholesterol were significantly reduced in HLE compared with HLC (p<.05). Also, TNF-α and MCP-1 were decreased in HLE compared with HLC (p<.05) by LPS-stimulation (0, 0.5, 1.0 μg/ml) and IL-6, IL-12p70 and IFN-r were decreased in HLE compared with HLC (p<.05) by LPS-stimulation (1.0 μg/ml). But resveratrol supplementation did not affect the result.
  
  
  CONCLUSIONS
  These findings suggest that exercise training has beneficial effects on body weight, total cholesterol, peritoneal macrophage and proinflammatory cytokine in high fat diet mice.
authors.affiliation: Department of Sports Science, Chungnam National University, Daejeon, Korea.
authors.forename: Hee Geun
authors.initials: HG
authors.lastname: Park
authors.affiliation: Department of Sports Science, Chungnam National University, Daejeon, Korea.
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Lee
authors.affiliation: Department of Sports Science, Chungnam National University, Daejeon, Korea.
authors.forename: Jong Kui
authors.initials: JK
authors.lastname: Jun
authors.affiliation: Department of Sports Science, Chungnam National University, Daejeon, Korea.
authors.forename: Wang Lok
authors.initials: WL
authors.lastname: Lee
keywords: High-fat diet; inflammation; macrophage; moderate exercise; resveratrol