title: Simultaneous stimulation and recording of cardiac depolarization enabled by high-frequency stimulation.
abstract: High-frequency stimulation techniques have been recently proposed for the pacing and control of excitability of cardiac tissues. This paper introduces a system designed specifically for such stimulation, and demonstrates the unique ability to record depolarization events on the same electrode used for stimulation, during the stimulus. Experimental results with HL-1 cardiomyocytes are presented, highlighting key concepts enabled by this system, such as direct strength-duration relationship measurement and beat-to-beat stimulation threshold monitoring following pacing onset or pharmacological modulation.
authors.forename: Laurent
authors.initials: L
authors.lastname: Giovangrandi
mesh_terms: D000200:Action Potentials; D000818:Animals; D016477:Artifacts; D002460:Cell Line; D004599:Electric Stimulation Therapy; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D006321:Heart / Q000502:physiology*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D051379:Mice; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000166:cytology*; D010138:Pacemaker, Artificial; D010363:Pattern Recognition, Automated; D012815:Signal Processing, Computer-Assisted