title: A metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by Rhizopus oryzae.
abstract: In this work, wheat bran (WB) was utilized as feedstock to synthesize fumaric acid by Rhizopus oryzae. Firstly, the pretreatment process of WB by dilute sulfuric acid hydrolysis undertaken at 100°C for 30min offered the best performance for fumaric acid production. Subsequently, through optimizing the seed culture medium, a suitable morphology (0.55mm pellets diameter) of R. oryzae was obtained. Furthermore, a metabolic-based approach was developed to profile the differences of intracellular metabolites concentration of R. oryzae between xylose (the abundant sugar in wheat bran hydrolysate (WBH)) and glucose metabolism. The xylitol, sedoheptulose 7-phosphate, ribulose 5-phosphate, glucose 6-phosphate, proline and serine were responsible for fumaric acid biosynthesis limitation in xylose fermentation. Consequently, regulation strategies were proposed, leading to a 149% increase in titer (up to 15.4g/L). Finally, by combinatorial regulation strategies the highest production was 20.2g/L from WBH, 477% higher than that of initial medium.
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 300072, People's Republic of China.
authors.forename: Guanyi
authors.initials: G
authors.lastname: Wang
authors.affiliation: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 300072, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 300072, People's Republic of China. Electronic address: jpwen@tju.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.affiliation: Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, People's Republic of China; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 300072, People's Republic of China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Jia
mesh_terms: D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D012233:Rhizopus / Q000378:metabolism*; D012274:Ribulosephosphates / Q000378:metabolism; D013403:Sugar Phosphates / Q000378:metabolism; D014993:Xylitol / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D005650:Fumarates; D012274:Ribulosephosphates; D013403:Sugar Phosphates; C020495:sedoheptulose 7-phosphate; C031524:ribulose 5-phosphate; C032005:fumaric acid; D014994:Xylose; D014993:Xylitol
keywords: Fumaric acid; Metabolic profiling; Rhizopus oryzae; Wheat bran; Xylose