title: High preexisting serological antibody levels correlate with diversification of the influenza vaccine response.
abstract: Reactivation of memory B cells allows for a rapid and robust immune response upon challenge with the same antigen. Variant influenza virus strains generated through antigenic shift or drift are encountered multiple times over the lifetime of an individual. One might predict, then, that upon vaccination with the trivalent influenza vaccine across multiple years, the antibody response would become more and more dominant toward strains consistently present in the vaccine at the expense of more divergent strains. However, when we analyzed the vaccine-induced plasmablast, memory, and serological responses to the trivalent influenza vaccine between 2006 and 2013, we found that the B cell response was most robust against more divergent strains. Overall, the antibody response was highest when one or more strains contained in the vaccine varied from year to year. This suggests that in the broader immunological context of viral antigen exposure, the B cell response to variant influenza virus strains is not dictated by the composition of the memory B cell precursor pool. The outcome is instead a diversified B cell response.
  
  
  OBJECTIVE
  Vaccine strategies are being designed to boost broadly reactive B cells present in the memory repertoire to provide universal protection to the influenza virus. It is important to understand how past exposure to influenza virus strains affects the response to subsequent immunizations. The viral epitopes targeted by B cells responding to the vaccine may be a direct reflection of the B cell memory specificities abundant in the preexisting immune repertoire, or other factors may influence the vaccine response. Here, we demonstrate that high preexisting serological antibody levels to a given influenza virus strain correlate with low production of antibody-secreting cells and memory B cells recognizing that strain upon revaccination. In contrast, introduction of antigenically novel strains generates a robust B cell response. Thus, both the preexisting memory B cell repertoire and serological antibody levels must be taken into consideration in predicting the quality of the B cell response to new prime-boost vaccine strategies.
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Andrews
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA Committee on Immunology, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Kaval
authors.initials: K
authors.lastname: Kaur
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA Committee on Immunology, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Noel T
authors.initials: NT
authors.lastname: Pauli
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA.
authors.forename: Yunping
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago, Chicago, Illinois, USA wilsonp@uchicago.edu.
authors.forename: Patrick C
authors.initials: PC
authors.lastname: Wilson
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D007251:Influenza, Human / Q000097:blood / Q000276:immunology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D009975:Orthomyxoviridae / Q000276:immunology* / Q000502:physiology; D014611:Vaccination; D055815:Young Adult
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines