title: Rs1800625 in the receptor for advanced glycation end products gene predisposes to sepsis and multiple organ dysfunction syndrome in patients with major trauma.
abstract: BACKGROUND
  The receptor for advanced glycation end products (RAGE) is a transmembrane receptor of the immunoglobulin superfamily, it plays pivotal roles in the pathogenesis of sepsis in several ways. Our previous study showed that rs1800625 (-429T/C) revealed a strong clinical relevance with sepsis morbidity rate and multiple organ dysfunction syndrome (MODS) in patients with major trauma. In this study, we enlarged the sample size, added two validation populations and examined the expression of RAGE on the surface of peripheral leukocytes to ex vivo lipopolysaccharide (LPS) stimulation in subjects with different genotypes.
  
  
  METHODS
  Rs1800625 was genotyped using pyrosequencing in 837 Chinese Han patients with major trauma in Chongqing. We then validated the clinical relevance in 340 Zhejiang and 347 Yunnan patients. The expression of RAGE on the surface of peripheral blood mononuclear cells was measured by flow cytometric analysis.
  
  
  RESULTS
  The results indicated that rs1800625 was significantly associated with sepsis morbidity rate and MODS in patients with major trauma in the Chongqing, Zhejiang and Yunnan districts. Patients with CC genotype had lower sepsis morbidity rate and MODS after major trauma. Furthermore, patients with CC genotype had significantly higher RAGE expression (P = 0.009).
  
  
  CONCLUSIONS
  The rs1800625 polymorphism is a functional single nucleotide polymorphism and confers host susceptibility to sepsis and MODS in patients with major trauma.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. zengling_1025@126.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. 57860275@qq.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. clgwjm@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. zhang-an-qiang@163.com.
authors.forename: An-qiang
authors.initials: AQ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. 3976341@qq.com.
authors.forename: Hai-yan
authors.initials: HY
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. 1059549576@qq.com.
authors.forename: Da-lin
authors.initials: DL
authors.lastname: Wen
authors.affiliation: Biochemistry and Molecular Biology Laboratory of Experiment Teaching Center, Chongqing Medical University, Fengyu Road, Chongqing, 401331, China. 245983564@qq.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. XTYang@163.com.
authors.forename: Xue-tao
authors.initials: XT
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. 2096415232@qq.com.
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Sun
authors.affiliation: Department of Emergency Medical Center, the Second Affiliated Hospital, Zhejiang University, 88 Jiefang Road, Zhejiang, 310009, China. zmhz@hotmail.com.
authors.forename: Mao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Kunming General Hospital, Chengdu Military of PLA, 212 Grand View Road, Kunming, Yunnan, 650032, China. 65783945@qq.com.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, 10 Changjiang Road, Yuzhong District, Daping, Chongqing, 400042, China. hellojjx@126.com.
authors.forename: Jian-xin
authors.initials: JX
authors.lastname: Jiang
mesh_terms: D000328:Adult; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009102:Multiple Organ Failure / Q000209:etiology / Q000235:genetics*; D009104:Multiple Trauma / Q000150:complications*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics*; D015203:Reproducibility of Results; D018805:Sepsis / Q000209:etiology / Q000235:genetics*
chemical_list: D000067759:Receptor for Advanced Glycation End Products