title: Overlapping local and long-range RNA-RNA interactions modulate dengue virus genome cyclization and replication.
abstract: The dengue virus genome is a dynamic molecule that adopts different conformations in the infected cell. Here, using RNA folding predictions, chemical probing analysis, RNA binding assays, and functional studies, we identified new cis-acting elements present in the capsid coding sequence that facilitate cyclization of the viral RNA by hybridization with a sequence involved in a local dumbbell structure at the viral 3' untranslated region (UTR). The identified interaction differentially enhances viral replication in mosquito and mammalian cells.
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
authors.forename: Luana
authors.initials: L
authors.lastname: de Borba
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
authors.forename: Sergio M
authors.initials: SM
authors.lastname: Villordo
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
authors.forename: Nestor G
authors.initials: NG
authors.lastname: Iglesias
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
authors.forename: Claudia V
authors.initials: CV
authors.lastname: Filomatori
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina.
authors.forename: Leopoldo G
authors.initials: LG
authors.lastname: Gebhard
authors.affiliation: Fundación Instituto Leloir-CONICET, Buenos Aires, Argentina agamarnik@leloir.org.ar.
authors.forename: Andrea V
authors.initials: AV
authors.lastname: Gamarnik
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009033:Culicidae / Q000821:virology; D004261:DNA Replication; D003716:Dengue Virus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D050436:Regulatory Elements, Transcriptional
chemical_list: D020413:3' Untranslated Regions; D036022:Capsid Proteins; D012367:RNA, Viral