title: Proteomic analysis of serum and urine of HIV-monoinfected and HIV/HCV-coinfected patients undergoing long term treatment with nevirapine.
abstract: Nevirapine (NVP) is an effective nonnucleoside reverse transcriptase inhibitor (NNRTI) of particular interest as it is often used in resource limited countries. However, one of the main concerns with the use of NVP is hepatotoxicity and elevation of liver enzymes as a consequence of highly active antiretroviral therapy (HAART) containing NVP is more often reported in HIV patients coinfected with hepatitis C virus than in HIV-monoinfected patients. To discover possible markers of NVP induced hepatotoxicity, serum and urine samples from twenty-five HIV or HIV/HCV patients, all of whom had received NVP continuously for at least four months, and healthy controls were subjected to in-solution or in-gel proteomic analysis. A total of 83 differentially regulated proteins consisted of 34 proteins identified in serum by in-solution analysis, 2 proteins identified from serum in a 2D gel electrophoresis analysis, and 47 proteins identified in urine in an in-solution analysis. Three proteins, namely, haptoglobin, Rho-related BTB domain containing protein 3, and death-associated protein kinase 3, were selected for further validation by Western blot analysis and results showed that haptoglobin has potential for further development as an additional marker of NVP induced hepatotoxicity.
authors.affiliation: Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Jeerang
authors.initials: J
authors.lastname: Wongtrakul
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Thananya
authors.identifier: 0000-0002-7183-1595
authors.initials: T
authors.lastname: Thongtan
authors.affiliation: Proteomics Research Laboratory, Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathum Thani 12120, Thailand.
authors.forename: Sittiruk
authors.initials: S
authors.lastname: Roytrakul
authors.affiliation: Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Benjawan
authors.initials: B
authors.lastname: Kumrapich
authors.affiliation: Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Kanokwan
authors.initials: K
authors.lastname: Janphen
authors.affiliation: Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Jutarat
authors.initials: J
authors.lastname: Praparattanapan
authors.affiliation: Department of Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Khuanchai
authors.initials: K
authors.lastname: Supparatpinyo
authors.affiliation: Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Phuttamonthol Sai 4, Nakorn Pathom 73170, Thailand.
authors.forename: Duncan R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D019380:Anti-HIV Agents / Q000008:administration & dosage / Q000009:adverse effects*; D015415:Biomarkers / Q000097:blood / Q000652:urine; D001798:Blood Proteins / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000652:urine; D060085:Coinfection / Q000097:blood* / Q000188:drug therapy / Q000652:urine; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy / Q000652:urine; D006526:Hepatitis C / Q000097:blood* / Q000188:drug therapy / Q000652:urine; D006801:Humans; D019829:Nevirapine / Q000008:administration & dosage / Q000009:adverse effects*; D011507:Proteinuria / Q000097:blood / Q000652:urine; D040901:Proteomics
chemical_list: D019380:Anti-HIV Agents; D015415:Biomarkers; D001798:Blood Proteins; D019829:Nevirapine; D000410:Alanine Transaminase