title: Curcumin inhibits gastric inflammation induced by Helicobacter pylori infection in a mouse model.
abstract: Helicobacter pylori (H. pylori) infection triggers a sequence of gastric alterations starting with an inflammation of the gastric mucosa that, in some cases, evolves to gastric cancer. Efficient vaccination has not been achieved, thus it is essential to find alternative therapies, particularly in the nutritional field. The current study evaluated whether curcumin could attenuate inflammation of the gastric mucosa due to H. pylori infection. Twenty-eight C57BL/6 mice, were inoculated with the H. pylori SS1 strain; ten non-infected mice were used as controls. H. pylori infection in live mice was followed-up using a modified 13C-Urea Breath Test (13C-UBT) and quantitative real-time polymerase chain reaction (PCR). Histologically confirmed, gastritis was observed in 42% of infected non-treated mice at both 6 and 18 weeks post-infection. These mice showed an up-regulation of the expression of inflammatory cytokines and chemokines, as well as of toll-like receptors (TLRs) and MyD88, at both time points. Treatment with curcumin decreased the expression of all these mediators. No inflammation was observed by histology in this group. Curcumin treatment exerted a significant anti-inflammatory effect in H. pylori-infected mucosa, pointing to the promising role of a nutritional approach in the prevention of H. pylori induced deleterious inflammation while the eradication or prevention of colonization by effective vaccine is not available.
authors.affiliation: Serviço de Medicina 4-Hospital de Santa Marta/Centro Hospitalar de Lisboa Central, Rua de Santa Marta, 50, 1169-024 Lisboa, Portugal. santosbox@netcabo.pt.
authors.forename: António M
authors.initials: AM
authors.lastname: Santos
authors.affiliation: CEDOC-Nova Medical School-Faculdade de Ciências Médicas Campo Mártires da Pátria 130, 1169-056 Lisboa, Portugal. maria.lopes@fcm.unl.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Lopes
authors.affiliation: Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal. monica.oleastro@insa.min-saude.pt.
authors.forename: Mónica
authors.initials: M
authors.lastname: Oleastro
authors.affiliation: Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal. ivaledegato@gmail.com.
authors.forename: Inês Vale
authors.initials: IV
authors.lastname: Gato
authors.affiliation: Bacteriology Laboratory, Bordeaux University, 146 rue Léo Saignat F-33000 Bordeaux, France. pauline-floch@hotmail.fr.
authors.forename: Pauline
authors.initials: P
authors.lastname: Floch
authors.affiliation: Bacteriology Laboratory, Bordeaux University, 146 rue Léo Saignat F-33000 Bordeaux, France. lucie.benejat@u-bordeaux2.fr.
authors.forename: Lucie
authors.initials: L
authors.lastname: Benejat
authors.affiliation: Serviço de Anatomia Patológica-Instituto Português de Oncologia Dr. Francisco Gentil, R. Prof. Lima Basto, 1099-023 Lisboa, Portugal. pchaves@ipolisboa.min-saude.pt.
authors.forename: Paula
authors.initials: P
authors.lastname: Chaves
authors.affiliation: Serviço de Anatomia Patológica-Instituto Português de Oncologia Dr. Francisco Gentil, R. Prof. Lima Basto, 1099-023 Lisboa, Portugal. tpereira@ipolisboa.min-saude.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Pereira
authors.affiliation: CEDOC-Nova Medical School-Faculdade de Ciências Médicas Campo Mártires da Pátria 130, 1169-056 Lisboa, Portugal. eseixas@igc.gulbenkian.pt.
authors.forename: Elsa
authors.initials: E
authors.lastname: Seixas
authors.affiliation: Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisboa, Portugal. jorge.machado@insa.min-saude.pt.
authors.forename: Jorge
authors.initials: J
authors.lastname: Machado
authors.affiliation: Serviço de Medicina 4-Hospital de Santa Marta/Centro Hospitalar de Lisboa Central, Rua de Santa Marta, 50, 1169-024 Lisboa, Portugal. antonioguerreiro@hotmail.com.
authors.forename: António S
authors.initials: AS
authors.lastname: Guerreiro
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D003474:Curcumin / Q000494:pharmacology*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000187:drug effects / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology* / Q000517:prevention & control*; D016481:Helicobacter Infections / Q000517:prevention & control*; D016480:Helicobacter pylori / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000893:Anti-Inflammatory Agents; C507780:Myd88 protein, mouse; D053594:Myeloid Differentiation Factor 88; D051193:Toll-Like Receptors; D003474:Curcumin