title: In vitro and in vivo evaluation of the developed PLGA/HAp/Zein scaffolds for bone-cartilage interface regeneration.
abstract: OBJECTIVE
  To investigate the effect of electronspun PLGA/HAp/Zein scaffolds on the repair of cartilage defects.
  
  
  METHODS
  The PLGA/HAp/Zein composite scaffolds were fabricated by electrospinning method. The physiochemical properties and biocompatibility of the scaffolds were separately characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), and fourier transform infrared spectroscopy (FTIR), human umbilical cord mesenchymal stem cells (hUC-MSCs) culture and animal experiments.
  
  
  RESULTS
  The prepared PLGA/HAp/Zein scaffolds showed fibrous structure with homogenous distribution. hUC-MSCs could attach to and grow well on PLGA/HAp/Zein scaffolds, and there was no significant difference between cell proliferation on scaffolds and that without scaffolds (P>0.05). The PLGA/HAp/Zein scaffolds possessed excellent ability to promote in vivo cartilage formation. Moreover, there was a large amount of immature chondrocytes and matrix with cartilage lacuna on PLGA/HAp/Zein scaffolds.
  
  
  CONCLUSIONS
  The data suggest that the PLGA/HAp/Zein scaffolds possess good biocompatibility, which are anticipated to be potentially applied in cartilage tissue engineering and reconstruction.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China.
authors.forename: Yong Xin
authors.initials: YX
authors.lastname: Lin
authors.affiliation: Department of Traumatology, HuiZhou Municipal Central Hospital, Huizhou 516000, Guangdong, China.
authors.forename: Zhi Yong
authors.initials: ZY
authors.lastname: Ding
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China.
authors.forename: Xiao Bin
authors.initials: XB
authors.lastname: Zhou
authors.affiliation: Department of Pediatrics, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, Guangdong, China.
authors.forename: Si Tao
authors.initials: ST
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510630, Guangdong, China.
authors.forename: De Ming
authors.initials: de M
authors.lastname: Xie
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China.
authors.forename: Zhi Zhong
authors.initials: ZZ
authors.lastname: Li
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong, China.
authors.forename: Guo Dong
authors.initials: GD
authors.lastname: Sun
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001846:Bone Development / Q000502:physiology*; D002356:Cartilage / Q000254:growth & development*; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000502:physiology; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012038:Regeneration / Q000502:physiology; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult; D015028:Zein / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D015028:Zein; D017886:Durapatite
keywords: Cartilage tissue engineering; Chondrocyte; Electrospun; PLGA/HAp/Zein grafts; hUC-MSCs