title: Potential of extracellular enzymes from Trametes versicolor F21a in Microcystis spp. degradation.
abstract: Studies have shown that microorganisms may be used to eliminate cyanobacteria in aquatic environments. The present study showed that the white-rot fungus Trametes versicolor F21a could degrade Microcystis aeruginosa. After T. versicolor F21a and Microcystis spp. were co-incubated for 60h, >96% of Microcystis spp. cells were degraded by T. versicolor F21a. The activities of extracellular enzymes showed that cellulase, β-glucosidase, protease, and laccase were vital to Microcystis spp. degradation in the early stage (0h to 24h), while β-glucosidase, protease, laccase, and manganese peroxidase in the late stage (24h to 60h). The positive and significant correlation of the degradation rate with these enzyme activities indicated that these enzymes were involved in the degradation rate of Microcystis spp. cells at different phases. It suggested that the extracellular enzymes released by T. versicolor F21a might be vital to Microcystis spp. degradation. The results of this study may be used to develop alternative microbial control agents for cyanobacterial control.
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China; Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, School of Material and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Gaozhong
authors.initials: G
authors.lastname: Pu
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Shao
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Cheng
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Cai
authors.affiliation: Perennial Plant Garden of Nanjing, Nanjing, 210000 China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. Electronic address: jiayong_2001@njnu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Jia
authors.affiliation: School of Life Science, Nanjing University, Nanjing 210093, China. Electronic address: tianxj@nju.edu.cn.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Tian
mesh_terms: D018533:Biomass; D004798:Enzymes / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism; D046931:Microcystis / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D055454:Trametes / Q000201:enzymology* / Q000502:physiology; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D004798:Enzymes; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase; D001617:beta-Glucosidase
keywords: Control; Cyanobacteria; Degradation; Enzyme activity; Fungus