title: miR-30 promotes thermogenesis and the development of beige fat by targeting RIP140.
abstract: Members of the microRNA (miR)-30 family have been reported to promote adipogenesis and inhibit osteogenesis, yet their role in the regulation of thermogenesis remains unknown. In this study, we show that miR-30b/c concentrations are greatly increased during adipocyte differentiation and are stimulated by cold exposure or the β-adrenergic receptor activator. Overexpression and knockdown of miR-30b and -30c induced and suppressed, respectively, the expression of thermogenic genes such as UCP1 and Cidea in brown adipocytes. Forced expression of miR-30b/c also significantly increased thermogenic gene expression and mitochondrial respiration in primary adipocytes derived from subcutaneous white adipose tissue, demonstrating a promoting effect of miRNAs on the development of beige fat. In addition, knockdown of miR-30b/c repressed UCP1 expression in brown adipose tissue in vivo. miR-30b/c targets the 3'-untranslated region of the receptor-interacting protein 140 (RIP140), and overexpression of miR-30b/c significantly reduced RIP140 expression. Consistent with RIP140 as a target of miR-30b/c in regulating thermogenic gene expression, overexpression of RIP140 greatly suppressed the promoting effect of miR-30b/c on the expression of UCP1 and Cidea in brown adipocytes. Taken together, the data from our study identify miR-30b/c as a key regulator of thermogenesis and uncover a new mechanism underlying the regulation of brown adipose tissue function and the development of beige fat.
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hu
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xiao
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Bangqi
authors.initials: B
authors.lastname: Yin
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Linyun
authors.initials: L
authors.lastname: He
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Meng
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Meijuan
authors.initials: M
authors.lastname: Dong
authors.affiliation: Metabolic Syndrome Research Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Key Laboratory of Diabetes Immunology, Ministry of Education, Institute of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China Department of Pharmacology, University of Texas Health Science Center, San Antonio, TX liuf@uthscsa.edu.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D052437:Adipocytes, Brown / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D003080:Cold Temperature; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D000078723:Nuclear Receptor Interacting Protein 1; D022722:Thermogenesis / Q000235:genetics / Q000502:physiology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D035683:MicroRNAs; C534715:Mirn30d microRNA, mouse; D009687:Nuclear Proteins; D000078723:Nuclear Receptor Interacting Protein 1