title: Isoforms of Hsp70-binding human LDL in adult Schistosoma mansoni worms.
abstract: Schistosoma mansoni is one of the most common parasites infecting humans. They are well adapted to the host, and this parasite's longevity is a consequence of effective escape from the host immune system. In the blood circulation, lipoproteins not only help to conceal the worm from attack by host antibodies but also act as a source of lipids for S. mansoni. Previous SEM studies showed that the low-density lipoprotein (LDL) particles present on the surface of adult S. mansoni worms decreased in size when the incubation time increased. In this study, immunocytochemical and proteomic analyses were used to locate and identify S. mansoni binding proteins to human plasma LDL. Ultrathin sections of adult worms were cut transversely from the anterior, medial and posterior regions of the parasite. Immunocytochemical experiments revealed particles of gold in the tegument, muscle region and spine in male worms and around vitelline cells in females. Immunoblotting and 2D-electrophoresis using incubations with human serum, anti-LDL antibodies and anti-chicken IgG peroxidase conjugate were performed to identify LDL-binding proteins in S. mansoni. Analysis of the binding proteins using LC-MS identified two isoforms of the Hsp70 chaperone in S. mansoni. Hsp70 is involved in the interaction with apoB in the cytoplasm and its transport to the endoplasmic reticulum. However, further studies are needed to clarify the functional role of Hsp70 in S. mansoni, mainly related to the interaction with human LDL.
authors.affiliation: Laboratório de Expressão Gênica em Eucariotos, Instituto de Química - Departamento de Bioquímica, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, sala 1200, São Paulo, SP, 05508-000, Brazil, adrianasap@gmail.com.
authors.forename: Adriana S A
authors.initials: AS
authors.lastname: Pereira
authors.forename: Marília G S
authors.initials: MG
authors.lastname: Cavalcanti
authors.forename: Russolina B
authors.initials: RB
authors.lastname: Zingali
authors.forename: José L
authors.initials: JL
authors.lastname: Lima-Filho
authors.forename: Maria E C
authors.initials: ME
authors.lastname: Chaves
mesh_terms: D000818:Animals; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D015801:Helminth Proteins / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D020033:Protein Isoforms; D040901:Proteomics; D012550:Schistosoma mansoni / Q000378:metabolism*; D012555:Schistosomiasis mansoni / Q000469:parasitology*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015801:Helminth Proteins; D008077:Lipoproteins, LDL; D020033:Protein Isoforms