title: Evaluation of three different concentrations of Chlorhexidine for their substantivity to human dentin.
abstract: OBJECTIVE
  To evaluate the substantivity of different concentrations of Chlorhexidine (CHX) to dentin disks prepared in-vitro.
  
  
  METHODS
  Sixty dentin disks were prepared from extracted human third molars and divided into three groups (each containing 20 disks). All the disks were partially demineralized, as per standard procedure. Group A specimens were then treated with 10 microliters of 0.02% Chlorhexidine, Group B specimens with 10 microliters of 0.2% Chlorhexidine, and Group C specimens were treated with 10 microliters of 2% Chlorhexidine. They were then incubated in 1 ml of Phosphatebufferedsaline PBS (pH 7.4). The substantivity was evaluated after 24 hours and one week of incubation. CHX concentration in the eluates was spectrophotometrically analyzed.
  
  
  RESULTS
  A significant amount of CHX was found retained on the dentin disks in Group B as compared to Group C. Also, Group A performed significantly better than group C. However, no statistically significant difference was observed between Group A and Group B.
  
  
  CONCLUSIONS
  Both 0.02% and 0.2% Chlorhexidine can be clinically recommended when being used for prolonging the durability of resin-dentin bond.
authors.affiliation: Department of Conservative Dentistry and Endodontics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
authors.forename: Harpreet
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Orthodontics and Dentofacial Orthopaedics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Kapoor
authors.affiliation: Department of Orthodontics and Dentofacial Orthopaedics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
authors.forename: Jaidev
authors.initials: J
authors.lastname: Dhillon
authors.affiliation: Department of Conservative Dentistry and Endodontics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Kaur
keywords: Bond strength; chlorhexidine; concentration