title: Clinical significance of quantitative monitoring and mutational analysis of BCR-ABL1 transcript in Philadelphia chromosome positive B lymphoblastic leukemia.
abstract: Quantitative detection of BCR-ABL1 transcript is essential in monitoring residual disease of Philadelphia chromosome positive B lymphoblastic leukemia (Ph+ B-LL). We studied the kinetics of BCR-ABL1 transcript in 41 Ph+ B-LL patients in correlation with their clinical outcome. A total of 23 patients achieved complete molecular remission at 6 months post-treatment. This was associated with a lower relapse risk and better overall survival. Likewise, sustainable complete molecular remission in 27 patients was associated with superior clinical outcome. Sporadic low level BCR-ABL1 was detected in 12 of 27 patients who had attained complete molecular remission. The relapse rate was significantly higher in non-transplant patients with persistent positive BCR-ABL1 than patients transplanted when BCR-ABL1 was detectable. All eight patients harboring ABL1 kinase domain mutations died of disease or were transferred to hospice care. We concluded that monitoring the level of BCR-ABL1 transcript after hematologic remission has predictive value to the long-term outcome.
authors.affiliation: a Department of Pathology and Laboratory Medicine , Emory University , Atlanta, Georgia.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Department of Pathology and Laboratory Medicine , Emory University , Atlanta, Georgia.
authors.forename: Ruan T
authors.initials: RT
authors.lastname: Ramjit
authors.affiliation: a Department of Pathology and Laboratory Medicine , Emory University , Atlanta, Georgia.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Hill
authors.affiliation: b Division of Hematology/Oncology, Winship Cancer Institute, Emory University , Atlanta, Georgia.
authors.forename: Martha
authors.initials: M
authors.lastname: Arellano
authors.affiliation: b Division of Hematology/Oncology, Winship Cancer Institute, Emory University , Atlanta, Georgia.
authors.forename: H Jean
authors.initials: HJ
authors.lastname: Khoury
authors.affiliation: a Department of Pathology and Laboratory Medicine , Emory University , Atlanta, Georgia.
authors.forename: Karen P
authors.initials: KP
authors.lastname: Mann
keywords: B Lymphoblastic Leukemia (B-LL); BCR-ABL1; Philadelphia chromosome; Tyrosine kinase inhibitor (TKI)