title: MiR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells.
abstract: MicroRNA-34a (miR-34a) is a direct transcriptional target of p53, and is downregulated in several different types of cancer. However, the underlying mechanism of the miR-34a effects in colorectal cancer is not well understood. In this study, we explored the role of miR-34a in cell invasion, migration, and apoptosis. Transient overexpression of miR-34a in SW480 cells caused a severe decrease in cell migration and invasion (both, p<0.05) compared to the control groups. Combining miR-34a transfection with 5-fluorouracil (5-FU) treatment further enhanced the inhibition in SW480 cell migration and invasion (both, p<0.05) compared to 5-FU treatment alone. These cellular changes were associated with upregulation of acetylated‑p53 (ac-p53) and p21 and downregulation of sirtuin 1 (SIRT1). These data demonstrate that miR-34a regulates the expression of a number of critical proteins involved in apoptosis, proliferation and the response to chemotherapy. In summary, miR-34a increases the sensitivity of colon cancer cells to 5-FU treatment through specific regulation of the SIRT1/p53 pathway.
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Mingguang
authors.initials: M
authors.lastname: Lai
authors.affiliation: Department of Internal Medicine, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Du
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Ruiyue
authors.initials: R
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Genhua
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Dingguo
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Zhenglei
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China.
authors.forename: Zicheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Shenzhen People's Hospital, Shenzhen, Guangdong 518020, P.R. China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D005472:Fluorouracil / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D015398:Signal Transduction / Q000187:drug effects; D056564:Sirtuin 1 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53; D056564:Sirtuin 1; D005472:Fluorouracil