title: TRPP2-dependent Ca2+ signaling in dorso-lateral mesoderm is required for kidney field establishment in Xenopus.
abstract: In Xenopus laevis embryos, kidney field specification is dependent on retinoic acid (RA) and coincides with a dramatic increase of Ca(2+) transients, but the role of Ca(2+) signaling in the kidney field is unknown. Here, we identify TRPP2, a member of the transient receptor potential (TRP) superfamily of channel proteins encoded by the pkd2 gene, as a central component of Ca(2+) signaling in the kidney field. TRPP2 is strongly expressed at the plasma membrane where it might regulate extracellular Ca(2+) entry. Knockdown of pkd2 in the kidney field results in the downregulation of pax8, but not of other kidney field genes (lhx1, osr1 and osr2). We further show that inhibition of Ca(2+) signaling with an inducible Ca(2+) chelator also causes downregulation of pax8, and that pkd2 knockdown results in a severe inhibition of Ca(2+) transients in kidney field explants. Finally, we show that disruption of RA results both in an inhibition of intracellular Ca(2+) signaling and of TRPP2 incorporation into the plasma membrane of kidney field cells. We propose that TRPP2-dependent Ca(2+) signaling is a key component of pax8 regulation in the kidney field downstream of RA-mediated non-transcriptional control of TRPP2.
authors.affiliation: Université Pierre et Marie Curie-Paris VI, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France CNRS, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France.
authors.forename: Mélinée
authors.initials: M
authors.lastname: Futel
authors.affiliation: Université Toulouse 3, Centre de Biologie du Développement, 118 route de Narbonne, F31062 Toulouse, Cedex 04, France CNRS UMR5547, Toulouse F31062, France jean-francois.riou@upmc.fr catherine.leclerc@univ-tlse3.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Leclerc
authors.affiliation: Université Pierre et Marie Curie-Paris VI, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France CNRS, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France.
authors.forename: Ronan
authors.initials: R
authors.lastname: Le Bouffant
authors.affiliation: Université Pierre et Marie Curie-Paris VI, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France CNRS, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Buisson
authors.affiliation: Université Toulouse 3, Centre de Biologie du Développement, 118 route de Narbonne, F31062 Toulouse, Cedex 04, France CNRS UMR5547, Toulouse F31062, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Néant
authors.affiliation: Université Pierre et Marie Curie-Paris VI, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France CNRS, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France.
authors.forename: Muriel
authors.initials: M
authors.lastname: Umbhauer
authors.affiliation: Université Toulouse 3, Centre de Biologie du Développement, 118 route de Narbonne, F31062 Toulouse, Cedex 04, France CNRS UMR5547, Toulouse F31062, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Moreau
authors.affiliation: Université Pierre et Marie Curie-Paris VI, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France CNRS, Equipe 'Signalisation et Morphogenèse', UMR7622-Biologie du Développement, 9, quai Saint-Bernard, 75005 Paris, France jean-francois.riou@upmc.fr catherine.leclerc@univ-tlse3.fr.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Riou
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000196:embryology*; D007668:Kidney / Q000166:cytology / Q000196:embryology*; D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors / Q000235:genetics / Q000378:metabolism*; D050396:TRPP Cation Channels / Q000235:genetics / Q000378:metabolism*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D000072636:PAX8 Transcription Factor; D051761:Paired Box Transcription Factors; C499500:Pax8 protein, Xenopus; D050396:TRPP Cation Channels; D029867:Xenopus Proteins
keywords: Ca2+ signaling; Pax8; Pronephros; TRPP2; Xenopus