title: Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry.
abstract: This work describes the electrical investigation of paclitaxel-treated HeLa cells using a custom-made microfluidic biosensor for whole cell analysis in continuous flow. We apply the method of differential electrical impedance spectroscopy to treated HeLa cells in order to elucidate the changes in electrical properties compared with non-treated cells. We found that our microfluidic system was able to distinguish between treated and non-treated cells. Furthermore, we utilize a model for electrical impedance spectroscopy in order to perform a theoretical study to clarify our results. This study focuses on investigating the changes in the electrical properties of the cell membrane caused by the effect of paclitaxel. We observe good agreement between the model and the obtained results. This establishes the proof-of-concept for the application in cell drug therapy.
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.) ; DELTA Dansk Elektronik, Lys & Akustik, Venlighedsvej 4, DK-2970 Hørsholm, Denmark.
authors.forename: Julie
authors.initials: J
authors.lastname: Kirkegaard
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.).
authors.forename: Casper Hyttel
authors.initials: CH
authors.lastname: Clausen
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.) ; DELTA Dansk Elektronik, Lys & Akustik, Venlighedsvej 4, DK-2970 Hørsholm, Denmark.
authors.forename: Romen
authors.initials: R
authors.lastname: Rodriguez-Trujillo
authors.affiliation: Department of Micro- and Nanotechnology, Technical University of Denmark, Oersteds Plads 345 East, DK-2800 Kgs. Lyngby, Denmark; E-Mails: julki@nanotech.dtu.dk (J.K.); romenrt@yahoo.es (R.R.-T.); Winnie.Svendsen@nanotech.dtu.dk (W.E.S.).
authors.forename: Winnie Edith
authors.initials: WE
authors.lastname: Svendsen
keywords: HeLa cells; cell properties; electrical impedance spectroscopy; paclitaxel