title: Viscoelastic and mechanical properties of hyaluronan films and hydrogels modified by carbodiimide.
abstract: This study investigated an effect of different ways of the preparation of insoluble hyaluronan material on its mechanical and viscoelastic properties. Hyaluronan (NaHy) of molecular weight Mw=500,000 g mol(-1) was modified with N-(3-dimethylaminopropyl-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), to be able absorb liquid without changing its mechanical properties. The modified, water insoluble NaHy materials were prepared in different geometry; as modified films and modified cylinders with exact dimensions. The occurrence of modification was confirmed by FT-IR (Fourier transform infrared spectroscopy) and (1)H NMR (proton nuclear magnetic resonance) spectroscopy and swelling test. The determined mechanical and viscoelastic properties of unmodified and modified hyaluronan revealed the high dependency of elasticity changes depending on the gel processing method. Moreover, NaHy gels in the cylindrical form with the sponge-like structure predominant them as a convenient geometry for application in a humid environment.
authors.affiliation: Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Material Engineering, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Gřundělová
authors.affiliation: Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria. Electronic address: adriana.gregorova@tugraz.at.
authors.forename: Adriana
authors.initials: A
authors.lastname: Gregorova
authors.affiliation: Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Material Engineering, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic.
authors.forename: Aleš
authors.initials: A
authors.lastname: Mráček
authors.affiliation: Tomas Bata University in Zlín, Faculty of Technology, The Department of Chemistry, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic.
authors.forename: Robert
authors.initials: R
authors.lastname: Vícha
authors.affiliation: Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Material Engineering, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Smolka
authors.affiliation: Tomas Bata University in Zlín, Faculty of Technology, Department of Physics and Material Engineering, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic; Centre of Polymer Systems, Tomas Bata University in Zlín, nám. T.G. Masaryka 5555, 760 01 Zlín, Czech Republic.
authors.forename: Antonín
authors.initials: A
authors.lastname: Minařík
mesh_terms: D004548:Elasticity; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013388:Succinimides / Q000737:chemistry; D013718:Tensile Strength; D014783:Viscosity; D014867:Water
chemical_list: D020100:Hydrogels; D013388:Succinimides; D014867:Water; D006820:Hyaluronic Acid; C001426:N-hydroxysuccinimide; D005022:Ethyldimethylaminopropyl Carbodiimide
keywords: Carbodiimide; Hyaluronan; Mechanical properties; Modification; Viscoelastic properties