title: Atg5 but not Atg7 in dendritic cells enhances IL-2 and IFN-γ production by Toxoplasma gondii-reactive CD4+ T cells.
abstract: The autophagy proteins (Atg) modulate not only innate but also adaptive immunity against pathogens. We examined the role of dendritic cell Atg5 and Atg7 in the production of IL-2 and IFN-γ by Toxoplasma gondii-reactive CD4(+) T cells. T. gondii-reactive mouse CD4(+) T cells exhibited unimpaired production of IL-2 and IFN-γ when stimulated with Atg7-deficient mouse dendritic cells that were infected with T. gondii or pulsed with T. gondii lysate antigens. In marked contrast, dendritic cells deficient in Atg5 induced diminished CD4(+) T cell production of IL-2 and IFN-γ. This defect was not accompanied by changes in costimulatory ligand expression on dendritic cells or impaired production of IL-12 p70, IL-1β or TNF-α. Knockdown of Irg6a in dendritic cells did not affect CD4(+) T cell cytokine production. These results indicate that Atg5 and Atg7 in dendritic cells play differential roles in the modulation of IL-2 and IFN-γ production by T. gondii-reactive CD4(+) T cells.
authors.affiliation: Department of Pathology, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA; Division of Infectious Diseases and HIV Medicine, Dept. of Medicine, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Liu
authors.affiliation: Department of Pathology, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA; Division of Infectious Diseases and HIV Medicine, Dept. of Medicine, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA; Department of Ophthalmology and Visual Sciences, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Van Grol
authors.affiliation: Department of Pathology, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA; Division of Infectious Diseases and HIV Medicine, Dept. of Medicine, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA; Department of Ophthalmology and Visual Sciences, Case Western Reserve University, 11100 Euclid Av., Cleveland, OH, 44106, USA. Electronic address: carlos.subauste@case.edu.
authors.forename: Carlos S
authors.initials: CS
authors.lastname: Subauste
mesh_terms: D000818:Animals; D001343:Autophagy / Q000276:immunology; D000071187:Autophagy-Related Protein 5; D000071193:Autophagy-Related Protein 7; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D004195:Disease Models, Animal; D007371:Interferon-gamma / Q000276:immunology*; D007376:Interleukin-2 / Q000276:immunology*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000276:immunology* / Q000627:therapeutic use; D014124:Toxoplasmosis, Animal / Q000276:immunology*
chemical_list: C495773:Atg5 protein, mouse; C502661:Atg7 protein, mouse; D000071187:Autophagy-Related Protein 5; D007376:Interleukin-2; D008869:Microtubule-Associated Proteins; D007371:Interferon-gamma; D000071193:Autophagy-Related Protein 7
keywords: Autophagy; Cytokine; Dendritic cells; T cells; Toxoplasma