title: Simultaneous determination of six triterpenic acids in some Chinese medicinal herbs using ultrasound-assisted dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection.
abstract: A novel analytical method was developed for simultaneous determination of six triterpenic acids using ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) follow by high-performance liquid chromatography (HPLC) with fluorescence detection. Six triterpenic acids (ursolic acid, oleanolic acid, betulinic acid, maslinic acid, betulonic acid and corosolic acid) were extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. After the extraction and nitrogen flushing, the extracts were rapidly derivatized with 2-(12,13-dihydro-7H-dibenzo[a,g]carbazol-7-yl)ethyl4-methylbenzenesulfonate. The main experimental parameters affecting extraction efficiency and derivatization yield were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The limits of detection (LODs) and the limits of quantification (LOQs) were in the range of 0.95-1.36 ng mL(-1) and 3.17-4.55 ng mL(-1), respectively. Under the optimum conditions, the method has been successfully applied for the analysis of triterpenic acids in six different traditional Chinese medicinal herbs.
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Wu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China. Electronic address: 61254368@163.com.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Ocean University, Zhanjiang, People's Republic of China.
authors.forename: Shucheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Na
authors.initials: N
authors.lastname: Hu
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China.
authors.forename: Yourui
authors.initials: Y
authors.lastname: Suo
authors.affiliation: Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, People's Republic of China; Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, People's Republic of China. Electronic address: jmyou6304@163.com.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005453:Fluorescence; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods; D008516:Medicine, Chinese Traditional / Q000379:methods; D009828:Oleanolic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D053978:Pentacyclic Triterpenes; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry*; D014465:Ultrasonics / Q000379:methods; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D012997:Solvents; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid; C118308:betulonic acid; C113861:corosolic acid; C412811:maslinic acid
keywords: High-performance liquid chromatography; Pre-column derivatization; Response surface methodology; Traditional Chinese medicinal herbs; Triterpenic acids; Ultrasound-assisted dispersive liquid–liquid microextraction