title: Cell selective chitosan microparticles as injectable cell carriers for tissue regeneration.
abstract: The detection, isolation and sorting of cells holds an important role in cell therapy and regenerative medicine. Also, injectable systems have been explored for tissue regeneration in vivo, because it allows repairing complex shaped tissue defects through minimally invasive surgical procedures. Here we report the development of chitosan microparticles with a size of 115.8 μm able to capture and expand a specific cell type that can also be regarded as an injectable biomaterial. Monoclonal antibodies against cell surface antigens specific to endothelial cells and stem cells were immobilized on the surface of the microparticles. Experimental results showed that particles bioconjugated with specific antibodies provide suitable surfaces to capture a target cell type and subsequent expansion of the captured cells. Primarily designed for an application in tissue engineering, three main challenges are accomplished with the herein presented microparticles: separation, scale-up expansion of specific cell type and successful use as an injectable system to form small tissue constructs in situ.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Custódio
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal.
authors.forename: M T
authors.initials: MT
authors.lastname: Cerqueira
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal.
authors.forename: A P
authors.initials: AP
authors.lastname: Marques
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal.
authors.forename: R L
authors.initials: RL
authors.lastname: Reis
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, AvePark, Zona Industrial da Gandra, S. Cláudio do Barco, 4806-909 Caldas das Taipas, Guimarães, Portugal; ICVS/3B's, PT Government Associated Laboratory, Braga, Guimarães, Portugal. Electronic address: jmano@dep.uminho.pt.
authors.forename: J F
authors.initials: JF
authors.lastname: Mano
mesh_terms: D000273:Adipose Tissue / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D005434:Flow Cytometry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D008863:Microspheres; D010316:Particle Size; D012038:Regeneration; D044968:Regenerative Medicine / Q000379:methods; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
chemical_list: D000911:Antibodies, Monoclonal; D001672:Biocompatible Materials; D001710:Biotin; D004247:DNA; D048271:Chitosan
keywords: Antibody immobilization; Cell separation; Chitosan microparticles; Functional materials; Injectable materials