title: Effect of 4-(N,N-dimethylamino)phenethyl alcohol on degree of conversion and cytotoxicity of photo-polymerized CQ-based resin composites.
abstract: The aim of this study was to evaluate the degree of conversion (DC) and the cytotoxicity of photo-cured experimental resin composites containing 4-(N,N-dimethylamino)phenethyl alcohol (DMPOH) combined to the camphorquinone (CQ) compared with ethylamine benzoate (EDAB). The resin composites were mechanically blended using 35 wt% of an organic matrix and 65 wt% of filler loading. To this matrix was added 0.2 wt% of CQ and 0.2 wt% of one of the reducing agents tested. 5x1 mm samples (n=5) were previously submitted to DC measurement and then pre-immersed in complete culture medium without 10% (v/v) bovine serum for 1 h or 24 h at 37 °C in a humidifier incubator with 5% CO2 and 95% humidity to evaluate the cytotoxic effects of experimental resin composites using the MTT assay on immortalized human keratinocytes cells. As a result of absence of normal distribution, the statistical analysis was performed using the nonparametric Kruskal-Wallis to evaluate the cytotoxicity and one-way analysis of variance to evaluate the DC. For multiple comparisons, cytotoxicity statistical analyses were submitted to Student-Newman-Keuls and DC analysis to Tukey's HSD post-hoc test (=0.05). No significant differences were found between the DC of DMPOH (49.9%) and EDAB (50.7%). 1 h outcomes showed no significant difference of the cell viability between EDAB (99.26%), DMPOH (94.85%) and the control group (100%). After 24 h no significant difference were found between EDAB (48.44%) and DMPOH (38.06%), but significant difference was found compared with the control group (p>0.05). DMPOH presented similar DC and cytotoxicity compared with EDAB when associated with CQ.
authors.affiliation: Department of Restorative Dentistry, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Dayane Carvalho Ramos Salles de
authors.initials: DC
authors.lastname: Oliveira
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Camila Batista da
authors.initials: CB
authors.lastname: Silva
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Bruno Vilela
authors.initials: BV
authors.lastname: Muniz
authors.affiliation: Department of Physiological Sciences, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Volpato
authors.affiliation: Department of Restorative Dentistry, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Ana Rosa
authors.initials: AR
authors.lastname: Costa
authors.affiliation: Department of Restorative Dentistry, Piracicaba Dental School, UNICAMP - University of Campinas, Piracicaba, SP, Brazil.
authors.forename: Mario Alexandre Coelho
authors.initials: MA
authors.lastname: Sinhoreti
mesh_terms: D002164:Camphor / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D002470:Cell Survival; D002478:Cells, Cultured; D003116:Color; D003188:Composite Resins / Q000737:chemistry*; D005021:Ethylamines / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D015603:Keratinocytes / Q000187:drug effects*; D008422:Materials Testing; D010626:Phenylethyl Alcohol / Q000737:chemistry*; D058105:Polymerization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003188:Composite Resins; D005021:Ethylamines; D002164:Camphor; D010626:Phenylethyl Alcohol; C553149:camphorquinone; C041564:ethylamine