title: Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection.
abstract: T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes.
authors.affiliation: State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. wuzhan1002@gmail.com jianhuijiang@hnu.edu.cn.
authors.forename: Zhen-Kun
authors.initials: ZK
authors.lastname: Wu
authors.forename: Dian-Ming
authors.initials: DM
authors.lastname: Zhou
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wu
authors.forename: Xia
authors.initials: X
authors.lastname: Chu
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D006108:Graphite / Q000737:chemistry; D007700:Kinetics; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D010087:Oxides / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide
chemical_list: D010087:Oxides; D006108:Graphite; D005090:Exodeoxyribonucleases; C046264:gene b exonuclease