title: Carrot cells: a pioneering platform for biopharmaceuticals production.
abstract: Carrot (Daucus carota L.) is of importance in the molecular farming field as it constitutes the first plant species approved to produce biopharmaceuticals for human use. In this review, features that make carrot an advantageous species in the molecular farming field are analyzed and a description of the developments achieved with this crop thus far is presented. A guide for genetic transformation procedures is also included. The state of the art comprises ten vaccine prototypes against Measles virus, Hepatitis B virus, Human immunodeficiency virus, Yersinia pestis, Chlamydia trachomatis, Mycobacterium tuberculosis, enterotoxigenic Escherichia coli, Corynebacterium diphtheria/Clostridium tetani/Bordetella pertussis, and Helicobacter pylori; as well as the case of the glucocerebrosidase, an enzyme used for replacement therapy, and other therapeutics. Perspectives for these developments are envisioned and innovations are proposed such as the use of transplastomic technologies-, hairy roots-, and viral expression-based systems to improve yields and develop new products derived from this advantageous plant species.
authors.affiliation: Laboratorio de Biofarmacéuticos Recombinantes, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 6, 78210, San Luis Potosí, SLP, Mexico, rosales.s@fcq.uaslp.mx.
authors.forename: Sergio
authors.initials: S
authors.lastname: Rosales-Mendoza
authors.forename: Marlene Anahí
authors.initials: MA
authors.lastname: Tello-Olea
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000517:prevention & control; D001702:Biopharmaceutics / Q000379:methods*; D018552:Daucus carota / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D056947:Enzyme Replacement Therapy; D005962:Glucosylceramidase / Q000627:therapeutic use; D006801:Humans; D058634:Molecular Farming / Q000379:methods*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D014612:Vaccines / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D014777:Virus Diseases / Q000517:prevention & control
chemical_list: D014612:Vaccines; D005962:Glucosylceramidase