title: The effect of SIRT1 protein knock down on PGC-1α acetylation during skeletal muscle contraction.
abstract: OBJECTIVE
  The purpose of this study was to investigate the effect of Sirtuin 1 (SIRT1) and General control nonderepressible 5 (GCN5) knock down on peroxisome proliferator- activated receptor gamma coactivator 1-alpha (PGC-1α) deacetylation during electrical stimulated skeletal muscle contraction.
  
  
  METHODS
  Skeletal muscle primary cell were isolated from C57BL/6 mice gastrocnemius and transfected lentiviral SIRT1 and GCN5 shRNA. Knock downed muscle cell were stimulated by electrical stimulation (1Hz, 3min) and collected for PGC-1α deceatylation assays. Immunoprecipitation performed for PGC-1α deacetylation, acetyl-lysine level was measured.
  
  
  RESULTS
  Our resulted showed SIRT1 knock down not influenced to PGC-1α deacetylation during electrical stimulation induced muscle contraction while GCN5 knock down decreased PGC-1α deacetylation significantly (p<0.05).
  
  
  CONCLUSIONS
  This study can be concluded that GCN5 is a critical factor for muscle contraction induced PGC-1α deacetylation.
authors.affiliation: Department of physical education, Chonbuk National University, Jeonju, Korea.
authors.forename: Dae Ryoung
authors.initials: DR
authors.lastname: Park
authors.affiliation: Department of physical education, Chonbuk National University, Jeonju, Korea.
authors.forename: Jeong Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Human Physiology, Korea National Sport University, Seoul, Korea.
authors.forename: Chang Keun
authors.initials: CK
authors.lastname: Kim
keywords: Deacetylation of PGC-1; GCN5; SIRT1; p-ACC; p-AMPK