title: Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas.
abstract: In order to re-build Man9GlcNAc2 clusters of the HIV gp120 glycoprotein, ∼2 nm gold glyconanoparticles (GNPs) were coated with the synthetic partial structures of Man9, the tetramannoside Manα1-2Manα1-2Manα1-3Manα1- and the pentamannoside Manα1-2Manα1-3[Manα1-2Manα1-6]Manα1-. Their interactions with the anti-HIV broadly neutralizing antibody 2G12 were studied by surface plasmon resonance (SPR)-based biosensors and saturation transfer difference (STD)-NMR spectroscopy. A synergistic effect of the tetra- and pentamannosides multimerized on a same GNP was observed. The assembly of these antennas of the gp120 high-mannose type glycan on GNPs provided superior binding to the anti-HIV antibody 2G12 with respect to GNPs carrying only the individual oligomannosides. The results presented in this work provide new molecular information on the interactions between clusters of oligomannosides and 2G12 that could help in the design of a carbohydrate-based vaccine against HIV.
authors.affiliation: Laboratory of Glyconanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, 28009 San Sebastian, Spain.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Chiodo
authors.affiliation: Laboratory of Glyconanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, 28009 San Sebastian, Spain.
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Enríquez-Navas
authors.affiliation: School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK.
authors.forename: Jesús
authors.initials: J
authors.lastname: Angulo
authors.affiliation: Laboratory of Glyconanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, 28009 San Sebastian, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), P° de Miramón 182, 28009 San Sebastian, Spain.
authors.forename: Marco
authors.initials: M
authors.lastname: Marradi
authors.affiliation: Laboratory of Glyconanotechnology, Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, 28009 San Sebastian, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), P° de Miramón 182, 28009 San Sebastian, Spain. Electronic address: spenades@cicbiomagune.es.
authors.forename: Soledad
authors.initials: S
authors.lastname: Penadés
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000080908:Broadly Neutralizing Antibodies; D006046:Gold / Q000737:chemistry*; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120 / Q000737:chemistry* / Q000276:immunology*; D008358:Mannose / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: C529157:2G12 monoclonal antibody; D000911:Antibodies, Monoclonal; D000080908:Broadly Neutralizing Antibodies; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D011134:Polysaccharides; D006046:Gold; D008358:Mannose
keywords: 2G12; Binding studies; Gold glyconanoparticles; High mannose-type glycans; SPR; STD-NMR