title: Effect of stab injury in the rat cerebral cortex on temporal pattern of expression of neuronal cytoskeletal proteins: an immunohistochemical study.
abstract: Compelling evidence now points to the critical role of the cytoskeleton in neurodegeneration. In the present study, using an immunohistochemical approach, we have shown that cortical stab injury (CSI) in adult Wistar rats significantly affects temporal pattern of expression of neurofilament proteins (NFs), a major cytoskeleton components of neurons, and microtubule-associated proteins (MAP2). At 3 days post-injury (dpi) most of the NFs immunoreactivity was found in pyknotic neurons and in fragmentized axonal processes in the perilesioned cortex. These cytoskeletal alterations became more pronounced by 10dpi. At the subcellular level CSI also showed significant impact on NFs and MAP-2 expression. Thus, at 3dpi most of the dendrites disappeared, while large neuronal somata appeared like open circles pointing to membrane disintegration. Conversely, at 10dpi neuronal perikarya and a few new apical dendrites were strongly labeled. Since aberrant NF phosphorylation is a pathological hallmark of many human neurodegenerative disorders, as well as is found after stressor stimuli, the present results shed light into the expression of neurofilaments after the stab brain injury.
authors.affiliation: Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" University of Belgrade, Belgrade, Serbia. Electronic address: irenam@ibiss.bg.ac.rs.
authors.forename: Irena
authors.initials: I
authors.lastname: Lavrnja
authors.affiliation: Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" University of Belgrade, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Savic
authors.affiliation: Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" University of Belgrade, Belgrade, Serbia.
authors.forename: Ana
authors.initials: A
authors.lastname: Parabucki
authors.affiliation: Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Dacic
authors.affiliation: Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Laketa
authors.affiliation: Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" University of Belgrade, Belgrade, Serbia.
authors.forename: Sanja
authors.initials: S
authors.lastname: Pekovic
authors.affiliation: Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" University of Belgrade, Belgrade, Serbia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Stojiljkovic
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D016900:Neurofilament Proteins / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D014951:Wounds, Stab / Q000378:metabolism* / Q000473:pathology
chemical_list: C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins
keywords: Cortical stab injury; Cytoskeleton; Immunohistochemistry; MAP-2; Neurofilaments