title: Development and calibration of a microfluidic biofilm growth cell with flow-templating and multi-modal characterization.
abstract: We report the development of a microfluidic flow-templating platform with multi-modal characterization for studies of biofilms and their precursor materials. A key feature is a special three inlet flow-template compartment, which confines and controls the location of biofilm growth against a template wall. Characterization compartments include Raman imaging to study the localization of the nutrient solutions, optical microscopy to quantify biofilm biomass and localization, and cyclic voltammetry for flow velocity measurements. Each compartment is tested and then utilized to make preliminary measurements.
authors.forename: Francois
authors.initials: F
authors.lastname: Paquet-Mercier
authors.forename: Adnane
authors.initials: A
authors.lastname: Karas
authors.forename: Muhammad
authors.initials: M
authors.lastname: Safdar
authors.forename: Nahid Babaei
authors.initials: NB
authors.lastname: Aznaveh
authors.forename: Mirpouyan
authors.initials: M
authors.lastname: Zarabadi
authors.forename: Jesse
authors.initials: J
authors.lastname: Greener
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002138:Calibration; D003198:Computer Simulation; D055664:Electrochemical Techniques; D044085:Microfluidics / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry; D055620:Optical Phenomena; D012212:Rheology; D013059:Spectrum Analysis, Raman