title: Protective effect of the combinations of glycyrrhizic, ferulic and cinnamic acid pretreatment on myocardial ischemia-reperfusion injury in rats.
abstract: The aim of this study was to find an effective drug cocktail pretreatment to protect myocardial tissue of the heart from ischemia-reperfusion (I/R) injury. The mechanisms underlying the effects of the drug cocktail were subsequently explored in order to expand the application of Dang-gui-si-ni-tang (DGSN), a Traditional Chinese Medicine. The active components of DGSN in the serum following oral administration were investigated using high-performance liquid chromatography. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) levels were then analyzed to show the effect of the active components in the treatment of myocardial I/R injury. AnL16 (44) orthogonal experiment was utilized to determine the most effective cocktail mix and the mechanism underlying the effect of this mix on myocardial I/R injury was investigated. It was observed that FCG, a mixture of glycyrrhizic (50 mg/kg), cinnamic (200 mg/kg) and ferulic (300 mg/kg) acid, was the optimal drug cocktail present in DGSN. This was absorbed into the blood following oral administration and was shown to decrease MDA levels and increase the activity of SOD. In conclusion, the findings suggest that FCG, a combination of active ingredients in the DGSN decoction, can be absorbed into the blood and protect the myocardium from I/R injury.
authors.affiliation: Department of Cardiology, Ninth Affiliated Hospital of the Medical College of Xi'an Jiaotong University and Railway Central Hospital of Xi'an, Xi'an, Shanxi 710054, P.R. China ; College of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Cardiology, Ninth Affiliated Hospital of the Medical College of Xi'an Jiaotong University and Railway Central Hospital of Xi'an, Xi'an, Shanxi 710054, P.R. China.
authors.forename: Jiping
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Nursing Science, Huanghuai University, Zhumadian, Henan 463000, P.R. China.
authors.forename: Hongkao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Qian
authors.affiliation: Department of Cardiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Renwang
authors.initials: R
authors.lastname: Jiang
authors.affiliation: College of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Cardiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Jianing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lei
authors.affiliation: College of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhao
keywords: cinnamic acid; ferulic acid; glycyrrhizic acid; ischemia-reperfusion cocktail