title: Transcriptome sequencing in a Tibetan barley landrace with high resistance to powdery mildew.
abstract: Hulless barley is an important cereal crop worldwide, especially in Tibet of China. However, this crop is usually susceptible to powdery mildew caused by Blumeria graminis f. sp. hordei. In this study, we aimed to understand the functions and pathways of genes involved in the disease resistance by transcriptome sequencing of a Tibetan barley landrace with high resistance to powdery mildew. A total of 831 significant differentially expressed genes were found in the infected seedlings, covering 19 functions. Either "cell," "cell part," and "extracellular region" in the cellular component category or "binding" and "catalytic" in the category of molecular function as well as "metabolic process" and "cellular process" in the biological process category together demonstrated that these functions may be involved in the resistance to powdery mildew of the hulless barley. In addition, 330 KEGG pathways were found using BLASTx with an E-value cut-off of <10(-5). Among them, three pathways, namely, "photosynthesis," "plant-pathogen interaction," and "photosynthesis-antenna proteins" had significant matches in the database. Significant expressions of the three pathways were detected at 24 h, 48 h, and 96 h after infection, respectively. These results indicated a complex process of barley response to powdery mildew infection.
authors.affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.
authors.forename: Xing-Quan
authors.initials: XQ
authors.lastname: Zeng
authors.affiliation: College of Forestry, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Luo
authors.affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.
authors.forename: Qi-Jun
authors.initials: QJ
authors.lastname: Xu
authors.affiliation: College of Forestry, Sichuan Agricultural University, Yaan, Sichuan 625014, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Bai
authors.affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Yuan
authors.affiliation: Barley Improvement and Yak Breeding Key Laboratory, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet 850002, China.
authors.forename: Nyima
authors.initials: N
authors.lastname: Tashi
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D030541:Databases, Genetic; D060467:Disease Resistance / Q000276:immunology*; D005091:Exons / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D001467:Hordeum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D007438:Introns / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D017423:Sequence Analysis, RNA / Q000379:methods*; D018609:Tibet; D059467:Transcriptome / Q000235:genetics*