title: In-vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae).
abstract: In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In-vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgL(-1)) solely or in combination with cytokinins BAP and KIN (1.0, 2.0 and 5.0 mgL(-1)). B5 medium supplemented with 1.0 mgL(-1) IBA plus 5.0 mgL(-1) BAP and MS medium fortified with 2.0 mgL(-1) IBA and 2.0 mgL(-1) BAP were the most favorable media for callus formation with the highest induction rate (96%). Maximum growth index (2.89 and 2.63) and maximum callus biomass (2.34 and 2.33 g fresh weight) were obtained from the callus cultured on B5 medium supplemented with 1.0 mgL(-1) IBA plus 5.0 mgL(-1) BAP and MS medium fortified with 1.0 mgL(-1) IBA plus 1.0 mgL(-1) KIN, respectively. Determination and quantification of RA in cultured calli were performed by HPLC UV/MS analysis. Calli induced from the plant and maintained on supplements of IBA and BAP in the absence of light produced RA 7.5% based on dry weight (DW). No differentiation was observed in any callus during the course of this study.
authors.affiliation: Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Sahraroo
authors.affiliation: Department of Horticulture, Faculty of Agriculture, University of Tehran, Karaj, Iran.
authors.forename: Mesbah
authors.initials: M
authors.lastname: Babalar
authors.affiliation: Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Mirjalili
authors.affiliation: Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Fattahi Moghaddam
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Samad
authors.initials: S
authors.lastname: Nejad Ebrahimi
keywords: Callus culture; HPLC UV/MS; Lamiaceae; Rosmarinic acid; Satureja sp