title: First characterisation of flavonoid- and diarylheptanoid-type antioxidant phenolics in Corylus maxima by HPLC-DAD-ESI-MS.
abstract: Corylus maxima Mill. (Betulaceae) leaves have been used in traditional medicine both internally and externally, nevertheless phytochemical exploration of the plant remains incomplete. In this study, the in vitro antioxidant activity and polyphenolic composition of the ethyl acetate and methanolic extracts of C. maxima leaves and bark are reported for the first time. The radical scavenging activities of the extracts were investigated by the ABTS and DPPH assays. All the extracts of C. maxima possessed notable antioxidant activity. By mean of a HPLC-DAD-ESI-TOF and a HPLC-DAD-ESI-MS/MS method, altogether twenty-two phenolics were tentatively characterised: one flavan derivative (1), seven flavonol derivatives (4, 6, 12, 13, 16, 20 and 21) and fourteen diarylheptanoids (2, 3, 5, 7-11, 14, 15, 17-19 and 22). The amount of the two main flavonoids - myricetin-3-O-rhamnoside (6) and quercetin-3-O-rhamnoside (13) - and two diarylheptanoids - oregonin (3) and hirsutenone (15) - in the extracts were determined by a validated HPLC-ESI-MS/MS method in multiple reaction monitoring (MRM) mode. Our results showed that C. maxima could be considered as a valuable source of pharmacologically important natural products that might contribute to the revaluation of the phytotherapeutical potential of the plant.
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Budapest H-1085, Hungary; Compound Profiling Laboratory, Gedeon Richter Plc., Budapest H-1475, Hungary. Electronic address: eszter.riethmuller@gmail.com.
authors.forename: Eszter
authors.initials: E
authors.lastname: Riethmüller
authors.affiliation: Department of Pharmaceutical Chemistry, Semmelweis University, Budapest H-1092, Hungary.
authors.forename: Gergő
authors.initials: G
authors.lastname: Tóth
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Budapest H-1085, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Alberti
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Budapest H-1085, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Végh
authors.affiliation: Faculty of Pharmacy, University of Ferrara, Ferrara I-44121, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Burlini
authors.affiliation: Compound Profiling Laboratory, Gedeon Richter Plc., Budapest H-1475, Hungary.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Könczöl
authors.affiliation: Compound Profiling Laboratory, Gedeon Richter Plc., Budapest H-1475, Hungary.
authors.forename: György Tibor
authors.initials: GT
authors.lastname: Balogh
authors.affiliation: Department of Pharmacognosy, Semmelweis University, Budapest H-1085, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Kéry
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D002396:Catechols / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D031211:Corylus / Q000737:chemistry*; D036381:Diarylheptanoids / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry*; D008362:Mannosides / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000975:Antioxidants; D002396:Catechols; D036381:Diarylheptanoids; D005419:Flavonoids; D008362:Mannosides; D010636:Phenols; D010936:Plant Extracts; C508777:hirsutenone; C529905:myricetin-3-O-rhamnoside; C409456:oregonin; C012526:quercitrin; D011794:Quercetin
keywords: Corylus maxima; Diarylheptanoid; Flavonoid; HPLC; MS