title: Rapid identification of apolipoprotein E genotypes by high-resolution melting analysis in Chinese Han and African Fang populations.
abstract: Apolipoprotein E (APOE) gene polymorphism can affect APOE gene transcription, serum lipid levels and repair of tissue damage, which could place individuals at serious risk of cardiovascular disease or certain infectious diseases. Recently, high-resolution melting (HRM) analysis was reported to be a simple, inexpensive, accurate and sensitive method for the genotyping or/and scanning of rare mutations. For this reason, an HRM analysis was used in the present study for APOE genotyping in the Southern Chinese Han and African Fang populations. A total of 100 healthy Southern Chinese Han and 175 healthy African Fang individuals attended the study. Polymerase chain reaction-DNA sequencing was used as a reference method for the genotyping of these samples. The six APOE genotypes could all be rapidly and efficiently identified by HRM analysis, and 100% concordance was found between the HRM analysis and the reference method. The allele frequencies of APOE in the Southern Chinese Han population were 7.0, 87.5 and 5.5% for ɛ2, ɛ3 and ɛ4, respectively. In the African Fang population, the allele frequencies of APOE were 24.3, 65.7 and 10.0% for ɛ2, ɛ3 and ɛ4, respectively. A statistically significant difference was found between the allele frequencies between the populations (P<0.05). In conclusion, the present study revealed the molecular characterization of APOE gene polymorphism in the Han population from the Chaozhou region of Southern China and the Fang population from Equatorial Guinea. The findings of the study indicated that HRM analysis could be used as an accurate and sensitive method for the rapid screening and identification of APOE genotypes in prospective clinical and population genetic analyses.
authors.affiliation: Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Xiu-Hui
authors.initials: XH
authors.lastname: Zhan
authors.affiliation: Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Guang-Cai
authors.initials: GC
authors.lastname: Zha
authors.affiliation: Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Jiao
authors.affiliation: Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Yang
authors.affiliation: Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Xiao-Fen
authors.initials: XF
authors.lastname: Zhan
authors.affiliation: The Chinese Medical Aid Team to Equatorial Guinea, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Jiang-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: The Chinese Medical Aid Team to Equatorial Guinea, Guangzhou, Guangdong 510000, P.R. China.
authors.forename: Dong-DE
authors.initials: DD
authors.lastname: Xie
authors.affiliation: Central Blood Transfusion Service, Malabo Regional Hospital, Malabo 999115, Equatorial Guinea.
authors.forename: Urbano Monsuy
authors.initials: UM
authors.lastname: Eyi
authors.affiliation: Central Blood Transfusion Service, Malabo Regional Hospital, Malabo 999115, Equatorial Guinea.
authors.forename: Rocio Apicante
authors.initials: RA
authors.lastname: Matesa
authors.affiliation: Central Blood Transfusion Service, Malabo Regional Hospital, Malabo 999115, Equatorial Guinea.
authors.forename: Maximo Miko Ondo
authors.initials: MM
authors.lastname: Obono
authors.affiliation: Central Blood Transfusion Service, Malabo Regional Hospital, Malabo 999115, Equatorial Guinea.
authors.forename: Carlos Sala
authors.initials: CS
authors.lastname: Ehapo
authors.affiliation: Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Er-Jia
authors.initials: EJ
authors.lastname: Wei
authors.affiliation: Department of Biology, Hanshan Normal University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zheng
authors.affiliation: Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Laboratory Medical Center, Chaozhou Central Hospital Affiliated to Southern Medical University, Chaozhou, Guangdong 521000, P.R. China ; Department of Internal Medicine, First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Lin
keywords: African Fang; Chinese Han; apolipoprotein E; genotype; high-resolution melting