title: Combined pretreatment using ozonolysis and ball milling to improve enzymatic saccharification of corn straw.
abstract: Two clean pretreatments, ozonolysis (OZ) and planetary ball milling (BM) were applied separately and in combination to improve the enzymatic hydrolysis of corn straw. Pretreatment of corn straw by OZ and BM alone improved the enzymatic hydrolysis significantly, primarily through delignification and decrystallization of cellulose, respectively. When combined, OZ-BM and BM-OZ pretreatments made the enzymatic hydrolysis more efficient. The glucose and xylose yield of corn straw treated with OZ for 90 min followed by BM for 8 min (OZ90-BM8) reached to 407.8 and 101.9 mg/g-straw, respectively under cellulase loading of 15 FPU/g-straw, which was fivefold more than that of untreated straw. Under much lower cellulase loading of 1.5 FPU/g-straw, the glucose and xylose yield of treated straw OZ90-BM8 remained at 416.0 and 108.4 mg/g-straw, respectively, while the yield of untreated straw decreased. These findings indicate that the combined OZ-BM can be used as a promising pretreatment for corn straw.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. Electronic address: shifeng@jiangnan.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Heji
authors.initials: H
authors.lastname: Xiang
authors.affiliation: National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Li
mesh_terms: D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D002480:Cellulase / Q000378:metabolism*; D003460:Crystallization; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D010126:Ozone / Q000494:pharmacology*; D010316:Particle Size; D014866:Waste Products; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry* / Q000187:drug effects
chemical_list: D014866:Waste Products; D010126:Ozone; D008031:Lignin; D014994:Xylose; D002480:Cellulase; D005947:Glucose
keywords: Ball milling; Corn straw; Enzymatic hydrolysis; Ozonolysis; Pretreatment