title: Faster in vivo clearance of human embryonic kidney than Chinese hamster ovary cell derived protein: Role of glycan mediated clearance.
abstract: This investigation used in vivo and in vitro tools to study pharmacokinetics and glycosylation of two monomeric antibodies produced either transiently by HEK293 cells or stably by Chinese hamster ovary cells, and demonstrated that higher in vivo clearance of human embryonic kidney antibody was due to higher glycosylation, thus higher mannose receptor mediated uptake.
authors.affiliation: Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Inc., Andover, MA 01810, USA. Electronic address: Mengmeng.Wang@pfizer.com.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Ishino
authors.affiliation: Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Inc., Andover, MA 01810, USA.
authors.forename: Alison
authors.initials: A
authors.lastname: Joyce
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: Amy
authors.initials: A
authors.lastname: Tam
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: Weili
authors.initials: W
authors.lastname: Duan
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: William S
authors.initials: WS
authors.lastname: Somers
authors.affiliation: Department of Global Biotherapeutics Technologies, Pfizer Inc., Cambridge, MA 02140, USA.
authors.forename: Ronald
authors.initials: R
authors.lastname: Kriz
authors.affiliation: Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Inc., Andover, MA 01810, USA.
authors.forename: Denise M
authors.initials: DM
authors.lastname: O'Hara
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000917:Antibody Formation; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D007700:Kinetics; D037181:Lectins, C-Type / Q000378:metabolism; D008351:Mannans / Q000378:metabolism* / Q000493:pharmacokinetics; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D000906:Antibodies; D037181:Lectins, C-Type; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface
keywords: Antibody; Clearance; Glycosylation; Mannan; Mannose receptor; Pharmacokinetics