title: Immunolocalization of a voltage-gated calcium channel β subunit in the tentacles and cnidocytes of the Portuguese man-of-war, Physalia physalis.
abstract: This study investigated the localization of a voltage-gated calcium channel (VGCC) β subunit in the tentacles and cnidocytes of the Portuguese man-of-war using confocal immunocytochemistry. An antibody specific to the Ca(2+) channel β subunit of the Portuguese-man-of-war (PpCaVβ) was generated, and characterized by Western immunoblotting. The antibody labeling was widespread in the ectoderm of cnidosacs of the tentacles. The binding of the antibody on isolated cnidocytes was distributed at the base of the cell and appeared as multiple strong fluorescent plaques located around the basal hemisphere of the cell. The distribution of PpCaVβ in the cnidocyte is consistent with previous studies on other hydrozoans that demonstrated that cnidocytes convey sensory information to other cnidocytes through chemical synapses in which the cnidocyte is pre-synaptic to elements of the animal's nervous system. Importantly and surprisingly, PpCaVβ did not localize to the apical surface of the cnidocyte where the exocytotic events involved in cnidocyst discharge are thought to take place.
authors.affiliation: Whitney Laboratory for Marine Bioscience and bouchardc@usf.edu.
authors.forename: Christelle
authors.initials: C
authors.lastname: Bouchard
authors.affiliation: Whitney Laboratory for Marine Bioscience and Dept. of Physiology and Functional Genomics, University of Florida, 9505 Ocean Shore Blvd., St. Augustine, Florida 32080.
authors.forename: Peter A V
authors.initials: PA
authors.lastname: Anderson
mesh_terms: D000825:Animal Structures / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D015220:Calcium Channels / Q000378:metabolism*; D037402:Hydrozoa / Q000166:cytology / Q000502:physiology*
chemical_list: D015220:Calcium Channels