title: Unsupervised feature construction and knowledge extraction from genome-wide assays of breast cancer with denoising autoencoders.
abstract: Big data bring new opportunities for methods that efficiently summarize and automatically extract knowledge from such compendia. While both supervised learning algorithms and unsupervised clustering algorithms have been successfully applied to biological data, they are either dependent on known biology or limited to discerning the most significant signals in the data. Here we present denoising autoencoders (DAs), which employ a data-defined learning objective independent of known biology, as a method to identify and extract complex patterns from genomic data. We evaluate the performance of DAs by applying them to a large collection of breast cancer gene expression data. Results show that DAs successfully construct features that contain both clinical and molecular information. There are features that represent tumor or normal samples, estrogen receptor (ER) status, and molecular subtypes. Features constructed by the autoencoder generalize to an independent dataset collected using a distinct experimental platform. By integrating data from ENCODE for feature interpretation, we discover a feature representing ER status through association with key transcription factors in breast cancer. We also identify a feature highly predictive of patient survival and it is enriched by FOXM1 signaling pathway. The features constructed by DAs are often bimodally distributed with one peak near zero and another near one, which facilitates discretization. In summary, we demonstrate that DAs effectively extract key biological principles from gene expression data and summarize them into constructed features with convenient properties.
authors.affiliation: Department of Genetics, Institute for Quantitative Biomedical Sciences, Norris Cotton Cancer Center, The Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Tan
authors.forename: Matthew
authors.initials: M
authors.lastname: Ung
authors.forename: Chao
authors.initials: C
authors.lastname: Cheng
authors.forename: Casey S
authors.initials: CS
authors.lastname: Greene
mesh_terms: D000465:Algorithms; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D019295:Computational Biology; D030541:Databases, Genetic / Q000706:statistics & numerical data; D005260:Female; D055106:Genome-Wide Association Study / Q000706:statistics & numerical data; D023281:Genomics / Q000706:statistics & numerical data; D006801:Humans; D051188:Knowledge Bases; D011379:Prognosis; D011960:Receptors, Estrogen / Q000378:metabolism; D015398:Signal Transduction; D016019:Survival Analysis; D000069558:Unsupervised Machine Learning
chemical_list: D011960:Receptors, Estrogen