title: Production of biobutanol from acid-pretreated corncob using Clostridium beijerinckii TISTR 1461: Process optimization studies.
abstract: Corncob is a potential feedstock in Thailand that can be used for fermentable sugar production through dilute sulfuric acid pretreatment and enzymatic hydrolysis. To recover high amounts of monomeric sugars from corncob, the sulfuric pretreatment conditions were optimized by using response surface methodology with three independent variables: sulfuric acid concentration, temperature, and time. The highest response of total sugars, 48.84 g/L, was found at 122.78°C, 4.65 min, and 2.82% (v/v) H2SO4. With these conditions, total sugars from the confirmation experiment were 46.29 g/L, with 5.51% error from the predicted value. The hydrolysate was used as a substrate for acetone-butanol-ethanol fermentation to evaluate its potential for microbial growth. The simultaneous saccharification and fermentation (SSF) showed that C. beijerinckii TISTR 1461 can generate acetone-butanol-ethanol products at 11.64 g/L (5.29 g/L acetone, 6.26 g/L butanol, and 0.09 g/L ethanol) instantly using sugars from the hydrolysed corncob with Novozymes 50013 cellulase enzyme without an overliming process.
authors.affiliation: a Department of General Sciences , Faculty of Science, Srinakharinwirot University , Bangkok , Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Boonsombuti
authors.affiliation: b Petroleum and Petrochemical College , Chulalongkorn University , Bangkok , Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Tangmanasakul
authors.affiliation: b Petroleum and Petrochemical College , Chulalongkorn University , Bangkok , Thailand.
authors.forename: J
authors.initials: J
authors.lastname: Nantapipat
authors.affiliation: c Institute of Biotechnology and Genetic Engineering , Chulalongkorn University , Bangkok , Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Komolpis
authors.affiliation: b Petroleum and Petrochemical College , Chulalongkorn University , Bangkok , Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Luengnaruemitchai
authors.affiliation: b Petroleum and Petrochemical College , Chulalongkorn University , Bangkok , Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Wongkasemjit
mesh_terms: D000096:Acetone / Q000378:metabolism; D000440:Butanols / Q000378:metabolism*; D002241:Carbohydrates; D002480:Cellulase / Q000737:chemistry / Q000378:metabolism; D046970:Clostridium beijerinckii / Q000254:growth & development / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D013464:Sulfuric Acids / Q000737:chemistry; D014961:X-Ray Diffraction; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000440:Butanols; D002241:Carbohydrates; D013464:Sulfuric Acids; D000096:Acetone; D000431:Ethanol; D002480:Cellulase; C033158:sulfuric acid
keywords: ABE fermentation; Clostridium beijerinckii; corncob; response surface methodology; simultaneous saccharification and fermentation; sulfuric acid pretreatment