title: RTG1- and RTG2-dependent retrograde signaling controls mitochondrial activity and stress resistance in Saccharomyces cerevisiae.
abstract: Mitochondrial retrograde signaling is a communication pathway between the mitochondrion and the nucleus that regulates the expression of a subset of nuclear genes that codify mitochondrial proteins, mediating cell response to mitochondrial dysfunction. In Saccharomyces cerevisiae, the pathway depends on Rtg1p and Rtg3p, which together form the transcription factor that regulates gene expression, and Rtg2p, an activator of the pathway. Here, we provide novel studies aimed at assessing the functional impact of the lack of RTG-dependent signaling on mitochondrial activity. We show that mutants defective in RTG-dependent retrograde signaling present higher oxygen consumption and reduced hydrogen peroxide release in the stationary phase compared to wild-type cells. Interestingly, RTG mutants are less able to decompose hydrogen peroxide or maintain viability when challenged with hydrogen peroxide. Overall, our results indicate that RTG signaling is involved in the hormetic induction of antioxidant defenses and stress resistance.
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, 05508-900 Cidade Universitária, SP, Brazil.
authors.forename: Nicole Quesada
authors.initials: NQ
authors.lastname: Torelli
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, 03828-000 Ermelino Matarazzo, SP, Brazil.
authors.forename: José Ribamar
authors.initials: JR
authors.lastname: Ferreira-Júnior
authors.affiliation: Departamento de Bioquímica, Universidade de São Paulo, 05508-900 Cidade Universitária, SP, Brazil. Electronic address: alicia@iq.usp.br.
authors.forename: Alicia J
authors.initials: AJ
authors.lastname: Kowaltowski
authors.affiliation: Departamento de Bioquímica, Universidade Federal de São Paulo, 04044-020 Vila Clementino, SP, Brazil. Electronic address: fmcunha@unifesp.br.
authors.forename: Fernanda Marques
authors.initials: FM
authors.lastname: da Cunha
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000222:Adaptation, Physiological / Q000235:genetics; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D010766:Phosphorylation; D021381:Protein Transport / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D047908:Intracellular Signaling Peptides and Proteins; C079378:RTG1 protein, S cerevisiae; C079379:RTG2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D006861:Hydrogen Peroxide
keywords: Free radicals; H(2)O(2); Hormesis; Mitochondria; Retrograde signaling