title: Differential effect of plant lipids on membrane organization: specificities of phytosphingolipids and phytosterols.
abstract: The high diversity of the plant lipid mixture raises the question of their respective involvement in the definition of membrane organization. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids, such as glycosylinositolphosphoceramides. This question was here addressed extensively by characterizing the order level of membrane from vesicles prepared using various plant lipid mixtures and labeled with an environment-sensitive probe. Fluorescence spectroscopy experiments showed that among major phytosterols, campesterol exhibits a stronger ability than β-sitosterol and stigmasterol to order model membranes. Multispectral confocal microscopy, allowing spatial analysis of membrane organization, demonstrated accordingly the strong ability of campesterol to promote ordered domain formation and to organize their spatial distribution at the membrane surface. Conjugated sterol forms, alone and in synergy with free sterols, exhibit a striking ability to order membrane. Plant sphingolipids, particularly glycosylinositolphosphoceramides, enhanced the sterol-induced ordering effect, emphasizing the formation and increasing the size of sterol-dependent ordered domains. Altogether, our results support a differential involvement of free and conjugated phytosterols in the formation of ordered domains and suggest that the diversity of plant lipids, allowing various local combinations of lipid species, could be a major contributor to membrane organization in particular through the formation of sphingolipid-sterol interacting domains.
authors.affiliation: From UMR1347 Agroécologie, ERL 6300 CNRS, Université de Bourgogne, 17 Rue Sully, BP 86510, 21065 Dijon Cedex, France.
authors.forename: Kevin
authors.initials: K
authors.lastname: Grosjean
authors.affiliation: the Laboratoire de Biogenèse Membranaire (LBM), CNRS, UMR 5200, F-33000 Villenave d'Ornon, France, the Laboratoire de Biogenèse Membranaire (LBM), Université de Bordeaux, UMR 5200, F-33000 Villenave d'Ornon, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Mongrand
authors.affiliation: the Laboratoire Procédés Alimentaires et Microbiologiques, AgroSup Dijon, F-21000 Dijon, France, and.
authors.forename: Laurent
authors.initials: L
authors.lastname: Beney
authors.affiliation: ERL 6300 CNRS, INRA, UMR1347 Agroécologie, 17 Rue Sully, BP 86510, 21065 Dijon Cedex, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Simon-Plas
authors.affiliation: From UMR1347 Agroécologie, ERL 6300 CNRS, Université de Bourgogne, 17 Rue Sully, BP 86510, 21065 Dijon Cedex, France, pgerbeau@dijon.inra.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Gerbeau-Pissot
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000032:analysis; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D021621:Imaging, Three-Dimensional; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry; D018613:Microscopy, Confocal; D008958:Models, Molecular; D010713:Phosphatidylcholines / Q000032:analysis; D010840:Phytosterols / Q000032:analysis; D010944:Plants / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013107:Sphingolipids / Q000032:analysis
chemical_list: D008055:Lipids; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010840:Phytosterols; D013107:Sphingolipids; D015060:1,2-Dipalmitoylphosphatidylcholine; C021273:campesterol; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine
keywords: Conjugated Forms of Phytosterol; GIPC; Glycerosphingolipid; Lipid Raft; Liquid Ordered/Disordered Phases; Membrane Reconstitution; Microscopic Imaging; Phospholipid Vesicle; Spatial Distribution of Ordered Domains; Sterol