title: Simple screening strategy with only water bath needed for the identification of insect-resistant genetically modified rice.
abstract: An informative, with simple instrument needed, rapid and easily updated strategy for the identification of insect-resistant genetically modified (GM) rice has been described. Such strategy is based on a parallel series of loop-mediated isothermal amplification (LAMP) reactions targeting the rice endogenous gene sucrose phosphate synthase (Sps), the top two most frequently used genetic elements (Agrobacterium tumefaciens nopaline synthase terminator (Nos) and Cauliflower mosaic virus 35S promoter (CaMV35S)), and an insect-resistant specific gene (Cry1Ac) and detected visually by phosphate ion (Pi)-induced coloration reaction. After a logical judgment of visible readouts has been obtained, three popular insect-resistant GM rice events in China can be successfully identified within 35 min, using either microwell strips or paper bases.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou 310058, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Yibin
authors.initials: Y
authors.lastname: Ying
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D018744:DNA, Plant / Q000235:genetics* / Q000302:isolation & purification; D004867:Equipment Design; D007313:Insecta / Q000502:physiology; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D012275:Oryza / Q000235:genetics* / Q000469:parasitology*; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000469:parasitology*
chemical_list: D018744:DNA, Plant