title: Chemical constituents, anticancer, antimicrobial and antioxidant activities of essential oil from Anaphalis lacteal grown in Qinghai-Tibet Plateau.
abstract: The chemical constituent of the essential oil from Anaphalis lacteal was determined; 31 compounds, representing 93.91% of the total oil, were identified by gas chromatography-mass spectrometry analysis. Three Gram-positive bacteria species, three Gram-negative bacteria species and four fungi were used to determine antimicrobial activity; the results revealed that the essential oil had a remarkable antimicrobial effect against bacteria and a susceptive effect against fungus. The oil also possessed more efficient free-radical scavenging activities than butylated hydroxytoluene (BHT) with 50% inhibitory concentration (IC50) value 31 μg/mL (40 μg/mL for BHT). MTT assay illustrated that the oil expressed certain effect in inhibiting the growth of HeLa and Hep-6 cancer cells.
authors.affiliation: a Department of Pharmaceutical Engineering , School of Chemistry and Chemical Engineering, Southeast University , Nanjing 211189 , P.R. China.
authors.forename: Shi-Jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liao
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Jin-Yue
authors.initials: JY
authors.lastname: Sun
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D019659:Asteraceae / Q000737:chemistry*; D045744:Cell Line, Tumor / Q000187:drug effects; D002681:China; D005658:Fungi / Q000187:drug effects; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D020128:Inhibitory Concentration 50; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry*; D018609:Tibet
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D009822:Oils, Volatile; D010938:Plant Oils
keywords: Anaphalis lacteal; anticancer; antimicrobial; antioxidant activities; chemical constituents; compositae