title: Dynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.
abstract: Lipid droplets (LDs) are ubiquitous organelles that serve as a neutral lipid reservoir and a hub for lipid metabolism. Manipulating LD formation, evolution, and mobilization in oleaginous species may lead to the production of fatty acid-derived biofuels and chemicals. However, key factors regulating LD dynamics remain poorly characterized. Here we purified the LDs and identified LD-associated proteins from cells of the lipid-producing yeast Rhodosporidium toruloides cultured under nutrient-rich, nitrogen-limited, and phosphorus-limited conditions. The LD proteome consisted of 226 proteins, many of which are involved in lipid metabolism and LD formation and evolution. Further analysis of our previous comparative transcriptome and proteome data sets indicated that the transcription level of 85 genes and protein abundance of 77 proteins changed under nutrient-limited conditions. Such changes were highly relevant to lipid accumulation and partially confirmed by reverse transcription-quantitative PCR. We demonstrated that the major LD structure protein Ldp1 is an LD marker protein being upregulated in lipid-rich cells. When overexpressed in Saccharomyces cerevisiae, Ldp1 localized on the LD surface and facilitated giant LD formation, suggesting that Ldp1 plays an important role in controlling LD dynamics. Our results significantly advance the understanding of the molecular basis of lipid overproduction and storage in oleaginous yeasts and will be valuable for the development of superior lipid producers.
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Gong
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Congyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Lin
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Shen
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Xie
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Fuquan
authors.initials: F
authors.lastname: Yang
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, Beijing, China zhaozb@dicp.ac.cn pliu@ibp.ac.cn.
authors.forename: Pingsheng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian, China Dalian National Laboratory for Clean Energy, Dalian Institute of Chemical Physics, CAS, Dalian, China zhaozb@dicp.ac.cn pliu@ibp.ac.cn.
authors.forename: Zongbao K
authors.initials: ZK
authors.lastname: Zhao
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism*; D050356:Lipid Metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014587:Ustilaginales / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D020543:Proteome