title: One-pot preparation of cross-linked amphiphilic fluorescent polymer based on aggregation induced emission dyes.
abstract: Facile one-pot preparation of cross-linked amphiphilic fluorescent polymer based on aggregation induced emission (AIE) dyes and 2-isocyanatoethyl methacrylate (IM) has been developed. This was carried out first by free radical polymerization between AIE monomer (PhE) and IM, and then polyethyleneimine (PEI) was introduced to obtain the cross-linked fluorescent polymer. The resulted cross-linked amphiphilic polymer was prone to self-assemble into stable nanoparticles in aqueous solution with surplus amino groups on the surface which made them highly water dispersible and can be further functionalized. The as-prepared fluorescent polymer nanoparticles (PhE-IM-PEI FPNs) were fully characterized by a series of techniques including (1)H NMR spectrum, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, dynamic light scattering, UV-vis absorption spectrum, and fluorescence spectra. Such FPNs demonstrated intense orange fluorescence with a high quantum yield of about 40%. Biocompatibility evaluation and cell uptake behavior of the nanoparticles were further investigated to explore their potential biomedical applications; the demonstrated excellent biocompatibility made them promising for cell imaging.
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. Electronic address: wangke007@iccas.ac.cn.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China; Department of Chemistry/Institute of Polymers, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Xiqi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Zengfang
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Chemistry and Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. Electronic address: weiyen@tsinghua.edu.cn.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D017953:Isocyanates / Q000737:chemistry / Q000494:pharmacology; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology; D015394:Molecular Structure; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013501:Surface-Active Agents / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D005456:Fluorescent Dyes; D017953:Isocyanates; D008689:Methacrylates; D011108:Polymers; D013501:Surface-Active Agents; C029845:2-isocyanatoethyl methacrylate; D011094:Polyethyleneimine
keywords: Aggregation induced emission; Amphiphilic fluorescent polymer nanoparticles; Cross-linked; Imaging