title: 4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase- and tyrosine ammonia lyase-expressing Streptomyces lividans.
abstract: Streptomyces lividans was adopted as a host strain for 4-vinylphenol (4VPh) production directly from cellulose. In order to obtain novel phenolic acid decarboxylase (PAD) expressed in S. lividans, PADs distributed among Streptomyces species were screened. Three novel PADs, derived from Streptomycessviceus, Streptomyceshygroscopicus, and Streptomycescattleya, were successfully obtained and expressed in S. lividans. S. sviceus PAD (SsPAD) could convert p-hydroxycinnamic acid (pHCA) to 4VPh more efficiently than the others both in vitro and in vivo. For 4VPh production directly from cellulose, l-tyrosine ammonia lyase derived from Rhodobacter sphaeroides and SsPAD were introduced into endoglucanase-secreting S. lividans, and the 4VPh biosynthetic pathway was constructed therein. The created transformants successfully produced 4VPh directly from cellulose.
authors.affiliation: Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kawai
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D003373:Coumaric Acids / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D011422:Propionates; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology; D048170:Streptomyces lividans / Q000235:genetics / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; D010636:Phenols; D011422:Propionates; D011994:Recombinant Proteins; D002482:Cellulose; D002262:Carboxy-Lyases; C417714:phenolic acid decarboxylase; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; C495469:p-coumaric acid; C030626:4-vinylphenol
keywords: 4-Vinylphenol; Cellulose; Phenolic acid decarboxylase; Streptomyces lividans