title: Tag-femtosecond laser-induced breakdown spectroscopy for the sensitive detection of cancer antigen 125 in blood plasma.
abstract: Successful treatment of cancers requires detecting early signs of the disease. One promising way to approach this is to develop minimally invasive tests for the sensitive and specific detection of biomarkers in blood. Irrespective of the detection approach one uses, this remains a challenging task because biomarkers are typically present in low concentrations and there are signals that interfere strongly with prevailing compounds of human fluids. In this paper, we show that elemental encoded particle assay coupled with femtosecond laser-induced breakdown spectroscopy for simultaneous multi-elemental analysis can significantly improve biomarker detectability. An estimated near single molecule per particle efficiency of this method leads to sensitive detection of ovarian cancer biomarker CA125 in human blood plasma. This work opens new ways for earlier detection of cancers and for multiplex assay developments in various analytical applications from proteomics, genomics, and neurology fields.
authors.affiliation: Optical Science Center for Applied Research and Applications and Department of Physics and Engineering, Delaware State University, Dover, DE, 19901, USA.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Markushin
authors.forename: Poopalasingam
authors.initials: P
authors.lastname: Sivakumar
authors.forename: Denise
authors.initials: D
authors.lastname: Connolly
authors.forename: Noureddine
authors.initials: N
authors.lastname: Melikechi
mesh_terms: D018394:CA-125 Antigen / Q000097:blood*; D006801:Humans; D007834:Lasers; D057230:Limit of Detection
chemical_list: D018394:CA-125 Antigen