title: Bioinformatic analysis of a PLP-dependent enzyme superfamily suitable for biocatalytic applications.
abstract: In this review we analyse structure/sequence-function relationships for the superfamily of PLP-dependent enzymes with special emphasis on class III transaminases. Amine transaminases are highly important for applications in biocatalysis in the synthesis of chiral amines. In addition, other enzyme activities such as racemases or decarboxylases are also discussed. The substrate scope and the ability to accept chemically different types of substrates are shown to be reflected in conserved patterns of amino acids around the active site. These findings are condensed in a sequence-function matrix, which facilitates annotation and identification of biocatalytically relevant enzymes and protein engineering thereof.
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany; KTH Royal Institute of Technology, School of Biotechnology, Division of Industrial Biotechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Fabian
authors.initials: F
authors.lastname: Steffen-Munsberg
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Clare
authors.initials: C
authors.lastname: Vickers
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany; Protein Biochemistry, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Hannes
authors.initials: H
authors.lastname: Kohls
authors.affiliation: KTH Royal Institute of Technology, School of Biotechnology, Division of Industrial Biotechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Land
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Mallin
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Alberto
authors.initials: A
authors.lastname: Nobili
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany.
authors.forename: Lilly
authors.initials: L
authors.lastname: Skalden
authors.affiliation: Bio-Prodict, Nieuwe Marktstraat 54E, 6511 AA Nijmegen, The Netherlands.
authors.forename: Tom
authors.initials: T
authors.lastname: van den Bergh
authors.affiliation: Bio-Prodict, Nieuwe Marktstraat 54E, 6511 AA Nijmegen, The Netherlands.
authors.forename: Henk-Jan
authors.initials: HJ
authors.lastname: Joosten
authors.affiliation: KTH Royal Institute of Technology, School of Biotechnology, Division of Industrial Biotechnology, AlbaNova University Center, SE-106 91 Stockholm, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Berglund
authors.affiliation: Protein Biochemistry, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. Electronic address: matthias.hoehne@uni-greifswald.de.
authors.forename: Matthias
authors.initials: M
authors.lastname: Höhne
authors.affiliation: Dept. of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, Greifswald University, Felix-Hausdorff-Str. 4, 17487 Greifswald, Germany. Electronic address: uwe.bornscheuer@uni-greifswald.de.
authors.forename: Uwe T
authors.initials: UT
authors.lastname: Bornscheuer
mesh_terms: D055162:Biocatalysis; D001709:Biotechnology; D019295:Computational Biology; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D000637:Transaminases
chemical_list: D011732:Pyridoxal Phosphate; D000637:Transaminases
keywords: Annotation; Biocatalysis; Bioinformatics; Enzyme discovery; PLP-dependent enzymes; Protein function; Transaminase