title: The vitamin D receptor localization and mRNA expression in ram testis and epididymis.
abstract: The objectives of present study were to investigate the presence of vitamin D receptor (VDR) in testis and epididymis of ram by polymerase chain reaction (PCR), to locate VDR in testis and epididymis by immunohistochemistry and to compare difference of VDR expression between testis and epididymis before and after sexual maturation by Real time-PCR and Western blot. The results showed that VDR exists in the testis and epididymis of ram while VDR protein in testis and epididymis was localized in Leydig cells, spermatogonial stem cells, spermatocytes, Sertoli cells and principal cells. For the adult ram, the amounts of VDR mRNA and VDR protein were less (p < 0.01) in testis than compared with caput, corpus and cauda epididymis. For prepubertal ram, the result showed the same trend (p < 0.01). However, the expression levels of VDR mRNA and VDR protein in caput, corpus, cauda epididymis and testis showed no significant difference (p > 0.05) between adult and prepubertal. In conclusion, VDR exists in testis and epididymis of ram, suggesting 1α,25-(OH)(2)VD(3) may play a role in ram reproduction.
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China. Electronic address: dongke051@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Animal Husbandry and Veterinary Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, PR China.
authors.forename: Guang
authors.initials: G
authors.lastname: Jin
authors.affiliation: Animal Husbandry and Veterinary Research Institute, Shanxi Academy of Agricultural Sciences, Taiyuan, PR China.
authors.forename: Yanrong
authors.initials: Y
authors.lastname: Xue
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Yao
authors.affiliation: College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, PR China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Yue
mesh_terms: D000818:Animals; D004822:Epididymis / Q000378:metabolism*; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics* / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012756:Sheep / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D012333:RNA, Messenger; D018167:Receptors, Calcitriol
keywords: Epididymis; Ram; Testis; VDR