title: Enhanced tolerance and remediation to mixed contaminates of PCBs and 2,4-DCP by transgenic alfalfa plants expressing the 2,3-dihydroxybiphenyl-1,2-dioxygenase.
abstract: Polychlorinated biphenyls (PCBs) and 2,4-dichlorophenol (2,4-DCP) generally led to mixed contamination of soils as a result of commercial and agricultural activities. Their accumulation in the environment poses great risks to human and animal health. Therefore, the effective strategies for disposal of these pollutants are urgently needed. In this study, genetic engineering to enhance PCBs/2,4-DCP phytoremediation is a focus. We cloned the 2,3-dihydroxybiphenyl-1,2-dioxygenase (BphC.B) from a soil metagenomic library, which is the key enzyme of aerobic catabolism of a variety of aromatic compounds, and then it was expressed in alfalfa driven by CaMV 35S promoter using Agrobacterium-mediated transformation. Transgenic line BB11 was selected out through PCR, Western blot analysis and enzyme activity assays. Its disposal and tolerance to both PCBs and 2,4-DCP were examined. The tolerance capability of transgenic line BB11 towards complex contaminants of PCBs/2,4-DCP significantly increased compared with non-transgenic plants. Strong dissipation of PCBs and high removal efficiency of 2,4-DCP were exhibited in a short time. It was confirmed expressing BphC.B would be a feasible strategy to help achieving phytoremediation in mixed contaminated soils with PCBs and 2,4-DCP.
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, Jilin 130021, People's Republic of China; College of Plant Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, Jilin 130021, People's Republic of China. Electronic address: renhejun@126.com.
authors.forename: Hejun
authors.initials: H
authors.lastname: Ren
authors.affiliation: College of Plant Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Pan
authors.affiliation: College of Plant Sciences, Jilin University, 5333 Xi'an Road, Changchun, Jilin 130062, People's Republic of China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Groud Water Resources and Environment of the Ministry of Education, College of Environment and Resources, Jilin University, 2519 Jiefang Road, Changchun, Jilin 130021, People's Republic of China.
authors.forename: Lanying
authors.initials: L
authors.lastname: Zhang
mesh_terms: D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000032:analysis* / Q000378:metabolism; D003001:Cloning, Molecular; D049308:Dioxygenases / Q000235:genetics / Q000378:metabolism*; D000455:Medicago sativa / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis* / Q000378:metabolism
chemical_list: D002733:Chlorophenols; D012989:Soil Pollutants; D011078:Polychlorinated Biphenyls; D049308:Dioxygenases; C074272:2,3-dihydroxybiphenyl oxygenase; C004762:2,4-dichlorophenol
keywords: 2,3-Dihydroxybiphenyl-1,2-dioxygenase; 2,4-Dichlorophenol; Phytoremediation; Polychlorinated biphenyls; Transgenic alfalfas