title: Behaviour of liposomes loaded with bovine serum albumin during in vitro digestion.
abstract: This study examined the stability of liposomes loaded with negatively charged protein (bovine serum albumin, BSA) during in vitro digestion. Zeta-potential and morphology measurements confirmed that BSA-loaded liposomes were successfully prepared, with an encapsulation efficiency of around 34%. The encapsulated BSA and the integrity of the liposomes remained unchanged with time when the liposomes were digested in a simulated gastric environment, suggesting that the liposomal membrane protected the entrapped BSA from pepsin hydrolysis. BSA-loaded liposomes exhibited lower stability in simulated intestinal fluid, as shown by damaged membranes and the release of free fatty acids. Also, lipolysis kinetics revealed that bile salts and ionic strength could facilitate a high level of free fatty acid release. This work further supplemented our knowledge about the effects of gastrointestinal digestion conditions on liposomal properties and provided valuable information for the design of liposome formulations for the food and health care industries.
authors.affiliation: College of Food and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand. Electronic address: a.m.ye@massey.ac.nz.
authors.forename: Aiqian
authors.initials: A
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, No. 235, Nanjing East Road, Nanchang 330047, Jiangxi, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, No. 235, Nanjing East Road, Nanchang 330047, Jiangxi, PR China.
authors.forename: Chengmei
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Food and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, PR China. Electronic address: hanjz99@zjgsu.edu.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Han
authors.affiliation: Riddet Institute, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
authors.forename: Harjinder
authors.initials: H
authors.lastname: Singh
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D008081:Liposomes; D012710:Serum Albumin, Bovine
keywords: Bovine serum albumin; Digestion; Liposome; Release kinetics