title: Comparative secretome analysis of rat stomach under different nutritional status.
abstract: Obesity is a major public health threat for many industrialised countries. Bariatric surgery is the most effective treatment against obesity, suggesting that gut derived signals are crucial for energy balance regulation. Several descriptive studies have proven the presence of gastric endogenous systems that modulate energy homeostasis; however, these systems and the interactions between them are still not well known. In the present study, we show for the first time the comparative 2-DE gastric secretome analysis under different nutritional status. We have identified 38 differently secreted proteins by comparing stomach secretomes from tissue explant cultures of rats under feeding, fasting and re-feeding conditions. Among the proteins identified, glyceraldehyde-3-phosphate dehydrogenase was found to be more abundant in gastric secretome and plasma after re-feeding, and downregulated in obesity. Additionally, two calponin-1 species were decreased in feeding state, and other were modulated by nutritional and metabolic conditions. These and other secreted proteins identified in this work may be considered as potential gastrokines implicated in food intake regulation.
  
  
  CONCLUSIONS
  The present work has an important impact in the field of obesity, especially in the regulation of body weight maintenance by the stomach. Nowadays, the most effective treatment in the fight against obesity is bariatric surgery, which suggests that stomach derived signals might be crucial for the regulation of the energy homeostasis. However, until now, the knowledge about the gastrokines and its mechanism of action has been poorly elucidated. In the present work, we had updated a previously validated explant secretion model for proteomic studies; this analysis allowed us, for the first time, to study the gastric secretome without interferences from other organs. We had identified 38 differently secreted proteins comparing ex vivo cultured stomachs from rats under feeding, fasting and re-feeding regimes. The results in the present article provide novel targets to study the role of the stomach in body weight and appetite regulation, and suggest new potential therapeutic targets for treating obesity.
authors.affiliation: Grupo Fisiopatologia Endocrina, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain.
authors.forename: Lucia L
authors.initials: LL
authors.lastname: Senin
authors.affiliation: CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain; Grupo Obesidomica, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
authors.forename: Arturo
authors.initials: A
authors.lastname: Roca-Rivada
authors.affiliation: Grupo Fisiopatologia Endocrina, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain; Grupo Obesidomica, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Castelao
authors.affiliation: Laboratorio de Proteómica, Instituto de Investigación Sanitaria de Santiago de Compostela (CHUS/SERGAS), Spain; MRC Protein Phosphorylation & Ubiquitylation Unit, University of Dundee, United Kingdom.
authors.forename: Jana
authors.initials: J
authors.lastname: Alonso
authors.affiliation: Grupo Fisiopatologia Endocrina, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain; Department of Physiology, Research Centre of Molecular Medicine and Chronic Diseases (CIMUS), Instituto de Investigacion Sanitaria Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
authors.forename: Cintia
authors.initials: C
authors.lastname: Folgueira
authors.affiliation: CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain; Laboratorio de Endocrinologia Molecular y Celular, Instituto de Investigacion Sanitaria de Santiago (IDIS), Complejo Hospitalario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; Department of Medicine, University of Santiago de Compostela, Spain.
authors.forename: Felipe F
authors.initials: FF
authors.lastname: Casanueva
authors.affiliation: CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain; Grupo Obesidomica, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain. Electronic address: maruxapardo@hotmail.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Pardo
authors.affiliation: Grupo Fisiopatologia Endocrina, Instituto de Investigacion Sanitaria de Santiago de Compostela (IDIS), Complexo Hospitalario Universitario de Santiago (CHUS/SERGAS), Santiago de Compostela, Spain; CIBER Fisiopatologia Obesidad y Nutricion, Instituto de Salud Carlos III, Santiago de Compostela, Spain. Electronic address: luisamaria.seoane@usc.es.
authors.forename: Luisa M
authors.initials: LM
authors.lastname: Seoane
mesh_terms: D000818:Animals; D005215:Fasting / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D009752:Nutritional Status; D020543:Proteome / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020543:Proteome
keywords: 2-DE; Gastrokine; Nutritional status; Obesity; Secretome; Stomach