title: In vivoimaging of adipose-derived mesenchymal stem cells in female nude mice after simulated childbirth injury.
abstract: The aim of the present study was to trackin vivothe distribution and survival of adipose-derived mesenchymal stem cells (ASCs) transplanted into female BALB/c nude mice following simulated childbirth injury, using green fluorescent protein and luciferase dual labeling, bioluminescent imaging (BLI) and histological evaluation. The results demonstrated that the dually labeled ASCs could be detected for up to eight weeksin vivo. The number of implanted cells decreased during the first three weeks, and then stabilized until the end of the experiment. According to the linear regression plot, ~27,621 implanted cells survived until eight weeks after implantation. Transplanted ASCs predominantly existed at the inoculation site of the vagina, with little or no spread to other organs. Histological analysis confirmed the survival of the engrafted ASCs. The study provided basic evidence that BLI techniques can be used to monitor ASCsin vivoin real time and in the long term. Through local administration, ASCs could survive in the long term to facilitate repair following pelvic-floor injury.
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Miao
authors.initials: M
authors.lastname: Dai
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Peirong
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Hou
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Yincheng
authors.initials: Y
authors.lastname: Teng
authors.affiliation: Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, P.R. China.
authors.forename: Qingkai
authors.initials: Q
authors.lastname: Wu
keywords: adipose-derived mesenchymal stem cells; bioluminescence imaging; simulated childbirth injury