title: Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy.
abstract: The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model. IC was induced by left coronary artery ligation, and baseline echocardiography was performed seven days later. Animals with a left ventricular ejection fraction (LVEF) of ≤40% were selected for the study. On the ninth day after IC was induced, the animals were randomized into the following experimental groups: UCSCs, UCBECs, UCSCs plus UCBECs, or vehicle (control). Thirty days after treatment, an echocardiographic analysis was performed, followed by euthanasia. The animals in all of the cell therapy groups, regardless of the cell type transplanted, had less collagen deposition in their heart tissue and demonstrated a significant improvement in myocardial function after IC. Furthermore, there was a trend of increasing numbers of blood vessels in the infarcted area. The median value of LVEF increased by 7.19% to 11.77%, whereas the control group decreased by 0.24%. These results suggest that UCSCs and UCBECs are promising cells for cellular cardiomyoplasty and can be an effective therapy for improving cardiac function following IC.
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil paula.h@pucpr.br paulahansensuss@gmail.com.
authors.forename: Paula H
authors.initials: PH
authors.lastname: Suss
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Luiz Guilherme A
authors.initials: LG
authors.lastname: Capriglione
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Fabiane
authors.initials: F
authors.lastname: Barchiki
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Lye
authors.initials: L
authors.lastname: Miyague
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Jackowski
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Letícia
authors.initials: L
authors.lastname: Fracaro
authors.affiliation: Carlos Chagas Institute, Oswaldo Cruz Foundation, FIOCRUZ/PR, Curitiba 81.350-010, Brazil.
authors.forename: Andressa V
authors.initials: AV
authors.lastname: Schittini
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Senegaglia
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Carmen L K
authors.initials: CL
authors.lastname: Rebelatto
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Márcia
authors.initials: M
authors.lastname: Olandoski
authors.affiliation: Carlos Chagas Institute, Oswaldo Cruz Foundation, FIOCRUZ/PR, Curitiba 81.350-010, Brazil.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Correa
authors.affiliation: Core for Cell Technology, Pontifícia Universidade Católica do Paraná, Curitiba 80.215-901, Brazil.
authors.forename: Paulo R S
authors.initials: PR
authors.lastname: Brofman
mesh_terms: D000818:Animals; D002469:Cell Separation; D036101:Cord Blood Stem Cell Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000637:transplantation*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells; D017202:Myocardial Ischemia / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous
keywords: Human umbilical cord-derived stromal cells; cell therapy; human umbilical cord blood-derived endothelial cells; ischemic cardiomyopathy