title: Multiple lysines combined in HIV-1 Vif determines the responsiveness to CBF-β.
abstract: The Vif (viral infectivity factor) protein of human immunodeficiency virus type-1 (HIV-1) is critical for HIV-1 infectivity. CBF-β is required for HIV-1 Vif function, as it increases the steady-state level of the HIV-1 Vif protein to promote host restriction factor APOBEC3 degradation. However, the precise mechanism by which CBF-β promotes HIV-1 Vif levels remains unclear. In the present study, we provided evidences that CBF-β promoted steady-state levels of HIV-1 Vif by inhibiting the degradation of HIV-1 Vif through the proteasome pathway. Our results reveal a new mechanism by which a cellular protein supports viral infectivity by inhibiting viral protein degradation.
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Hexing Road, Harbin 150040, PR China. Electronic address: aiyouwei@nibs.ac.cn.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Ai
authors.affiliation: College of Wildlife Resources, Northeast Forestry University, Hexing Road, Harbin 150040, PR China. Electronic address: jianzhangma@163.com.
authors.forename: Jianzhang
authors.initials: J
authors.lastname: Ma
mesh_terms: D019943:Amino Acid Substitution; D050658:Core Binding Factor beta Subunit / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D015658:HIV Infections / Q000235:genetics / Q000378:metabolism / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D006801:Humans; D008239:Lysine / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D059748:Proteolysis; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D054320:vif Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: C493692:CBFB protein, human; D050658:Core Binding Factor beta Subunit; D011994:Recombinant Proteins; D054320:vif Gene Products, Human Immunodeficiency Virus; C514847:vif protein, Human immunodeficiency virus 1; D046988:Proteasome Endopeptidase Complex; D008239:Lysine
keywords: A3G; CBF-β; HIV-1; Proteasome pathway; Vif