title: Spleen-specific isoforms of Pax5 and Ataxin-7 as potential proteomic markers of lymphoma-affected spleen.
abstract: The splenomegaly, enlargement of spleen, has been observed in several diseases. It has been intended to evaluate histochemical alterations, spleen-specific enzymatic and proteomic markers during splenomegaly, and lympho-proliferative disorders from spleen of mice bearing Dalton's lymphoma. The higher expression of c-fos, c-jun, and MAPK testifies proliferation of lymphocytes. The lower expression of Pax5, higher expression of CD3, and the presence of additional form of Zap-70 suggest hypertrophy of follicles and splenomegaly influenced by weak B-cell receptor-mediated signaling, but activated T-cell receptor-mediated signaling. Simultaneously, lower levels of SOD, NDR2, and MIB2 and higher expression levels of Ataxin-7 and LDH also suggest impact of stress either as a cause or effect of cell proliferation. Spleen-specific isoform of Pax5, NDR2, MIB2, and Ataxin-7 can be considered as spleen-specific unique molecular markers for the evaluation of splenomegaly and lympho-proliferative disorders.
authors.affiliation: Department of Zoology, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Brij
authors.initials: B
authors.lastname: Bharti
authors.forename: Rajnikant
authors.initials: R
authors.lastname: Mishra
mesh_terms: D000818:Animals; D000067719:Ataxin-7; D014408:Biomarkers, Tumor / Q000378:metabolism*; D008223:Lymphoma / Q000378:metabolism*; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009928:Organ Specificity; D051757:PAX5 Transcription Factor / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D013154:Spleen / Q000378:metabolism* / Q000473:pathology
chemical_list: D000067719:Ataxin-7; C000593831:Atxn7 protein, mouse; D014408:Biomarkers, Tumor; D009419:Nerve Tissue Proteins; D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse; D020033:Protein Isoforms; D020543:Proteome; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun