title: Listeria monocytogenes batch culture growth response to metabolic inhibitors.
abstract: In certain environments nutrient and energy sources available to microorganisms can be limited. Foodborne pathogens must efficiently adapt in order to be successfully transmitted through the food chain to their hosts. For the intracellular foodborne pathogen Listeria monocytogenes, little is known regarding its response to nutrient/energy-limiting conditions. The alternative stress responsive sigma factor σ(B) has been reported to contribute to survival under specific stresses. Therefore, the effects of several metabolic inhibitors on growth of L. monocytogenes wild-type and a ΔsigB mutant were examined. In the absence of inhibitors, both strains reached stationary phase after 18 h at 23°C and 10 h at 37°C. All of the metabolic inhibitors slowed growth of either strain, with few differences observed among the different inhibitors.
authors.affiliation: a Department of Food Science and Center for Food Safety , University of Arkansas , Fayetteville , Arkansas , USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Milillo
authors.forename: B
authors.initials: B
authors.lastname: Lungu
authors.forename: C A
authors.initials: CA
authors.lastname: O'Bryan
authors.forename: S E
authors.initials: SE
authors.lastname: Dowd
authors.forename: A
authors.initials: A
authors.lastname: Muthaiyan
authors.forename: M G
authors.initials: MG
authors.lastname: Johnson
authors.forename: S C
authors.initials: SC
authors.lastname: Ricke
mesh_terms: D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001149:Arsenates / Q000494:pharmacology; D018053:Arsenites / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005516:Food Microbiology; D007461:Iodoacetates / Q000494:pharmacology; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development*; D010766:Phosphorylation / Q000187:drug effects; D011190:Potassium Cyanide / Q000494:pharmacology; D017670:Sodium Compounds / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D001149:Arsenates; D018053:Arsenites; D004791:Enzyme Inhibitors; D007461:Iodoacetates; D017670:Sodium Compounds; C017947:sodium arsenite; C009277:sodium arsenate; D012969:Sodium Fluoride; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol
keywords: Listeria monocytogenes; iodoacetate; metabolic inhibitors; potassium cyanide; sodium arsenate.