title: Bioactive indole alkaloids isolated fromAlstonia angustifolia.
abstract: Bioassay-guided fractionation was conducted on a CHCl3-soluble extract of the stem bark ofAlstonia angustifolia(Apocynaceae) collected in Vietnam using the HT-29 human colon cancer cell line, and led to the isolation of a new sarpagine-type indole alkaloid (1), together with nine known alkaloids, including four macroline-derived alkaloids (2-5), a sarpagine-type alkaloid (6), and four macroline-pleiocarpamine bisindole alkaloids (7-10). The structure of the new compound (1) was determined on the basis of spectroscopic data interpretation. Compounds1-10were evaluatedin vitrofor their NF-κB (p65) inhibitory activity against the Hela cells in an ELISA assay. The new sarpagine alkaloid,N(4)-methyltalpinine (1), was found to show significant NF-κB inhibitory activity (ED50= 1.2 µM). Furthermore, all the isolates (1-10) were evaluatedin vitrofor their antileishmanial activity, and compounds (1-4, 6and8-10) exhibited leishmaniacidal activity against promastigotes ofLeishmania mexicana.
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, OH 43210, United States.
authors.forename: Li
authors.initials: L
authors.lastname: Pan
authors.affiliation: Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: César
authors.initials: C
authors.lastname: Terrazas
authors.affiliation: Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Ulyana
authors.initials: U
authors.lastname: Muñoz Acuña
authors.affiliation: Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Tran Ngoc
authors.initials: TN
authors.lastname: Ninh
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, OH 43210, United States.
authors.forename: Heebyung
authors.initials: H
authors.lastname: Chai
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, OH 43210, United States ; Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Esperanza J
authors.initials: EJ
authors.lastname: Carcache de Blanco
authors.affiliation: Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, United States ; Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Djaja D
authors.initials: DD
authors.lastname: Soejarto
authors.affiliation: Department of Pathology, College of Medicine, The Ohio State University, Columbus, OH 43210, United States.
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Satoskar
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, OH 43210, United States.
authors.forename: A Douglas
authors.initials: AD
authors.lastname: Kinghorn
keywords: Alstonia angustifolia (Apocynaceae); NF-κB (p65) inhibitory activity; antileishmanial activity; cytotoxicity; macroline-pleiocarpamine bisindole alkaloid; macroline-type alkaloid; sarpagine-type alkaloid; stem bark