title: Toxicological assessment of Ashitaba Chalcone.
abstract: The plant Angelica keiskei contains two main physiologically active flavonoid chalcones, 4-hydroxyderricin and xanthoangelol. Known as ashitaba in Japan, powder from the sap is widely consumed for its medicinal properties in Asia as a dietary supplement. Limited previously reported mammalian studies were without evidence of toxicity. GLP studies reported here, including a bacterial reverse mutation assay, a chromosome aberration assay, and an in vivo micronucleus assay are negative for genotoxicity. A GLP- compliant 90-day repeated oral gavage study of ashitaba yellow sap powder containing 8.45% chalcones in Sprague Dawley rats resulted in expected known physiological effects on coagulation parameters and plasma lipids at 300 and 1000 mg/kg/day. Ashitaba-related pathology included a dose-related male rat-specific alpha 2-urinary globulin nephropathy at 100, 300, and 1000 mg/kg/day and jejunal lymphangiectasia in both sexes at 1000 mg/kg/day. All other study parameters and histopathological changes were incidental or not of toxicological concern. Based on these studies ashitaba chalcone powder is not genotoxic with a NOAEL of 300 mg/kg in male and female rats.
authors.affiliation: Maronpot Consulting LLC, 1612 Medfield Road, Raleigh, NC 27607, United States. Electronic address: Maronpot@me.com.
authors.forename: Robert R
authors.initials: RR
authors.lastname: Maronpot
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D016466:CHO Cells; D002599:Chalcone / Q000031:analogs & derivatives* / Q000633:toxicity; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D051379:Mice; D019055:No-Observed-Adverse-Effect Level; D010936:Plant Extracts / Q000633:toxicity*; D016032:Randomized Controlled Trials as Topic; D051381:Rats; D017207:Rats, Sprague-Dawley; D059550:Toxicity Tests, Subchronic
chemical_list: D010936:Plant Extracts; C068243:4-hydroxyderricin; D002599:Chalcone; C068244:xanthoangelol
keywords: 4-Hydroxyderricin; Alpha 2-urinary globulin nephropathy; Chalcones; Flavonoids; Lymphangiectasia; Xanthoangelol