title: Cranial bone regeneration after cranioplasty using cryopreserved autogenous bone by a programmed freezer with a magnetic field in rats.
abstract: The purpose of this study was to develop a bone tissue bank using a programmed freezer with a magnetic field. Parietal bones were removed from rats and used for organ culture examination (non-cryopreserved, cryopreserved with a magnetic field (CAS) and cryopreserved without a magnetic field group). Next, other parietal bones were used for histological examination. The cryopreserved bones by a CAS freezer and dried bones were transplanted respectively. Control bones were replanted without cryopreservation. Animals were sacrificed at 4, 8, 12 and 24 weeks after surgery. After organ culture, the isolated osteoblasts from parietal bones which were cryopreserved by a CAS freezer can survive and proliferate as much as non-cryopreserved group. Histological examinations showed new bone formation in control and CAS group. These results suggest that bone tissue cryopreservation by CAS freezer can be successfully used for bone grafting which may be a novel option for regeneration medicine.
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan. mkaku@hiroshima-u.ac.jp.
authors.forename: Masato
authors.initials: M
authors.lastname: Kaku
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Koseki
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Shunich
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Sumi
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Hanaka
authors.initials: H
authors.lastname: Shikata
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Shotoku
authors.initials: S
authors.lastname: Kojima
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Masahide
authors.initials: M
authors.lastname: Motokawa
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Fujita
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Tanimoto
authors.affiliation: Department of Orthodontics and Craniofacial Developmental Biology, Hiroshima University Graduate School of Biomedical Sciences, Minami-ku, Hiroshima, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Tanne
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D015925:Cryopreservation / Q000379:methods*; D060526:Magnetic Fields; D008297:Male; D009924:Organ Culture Techniques; D010294:Parietal Bone / Q000502:physiology* / Q000637:transplantation* / Q000648:ultrastructure; D051381:Rats; D011916:Rats, Inbred F344; D014015:Tissue Banks