title: Synthesis of highly fluorescent hydrophobic carbon dots by hot injection method using Paraplast as precursor.
abstract: We have reported synthesis of bright blue colored hydrophobic carbon dots (hC-dots) using highly pure blend of polymers called Paraplast. We developed a hot injection method for making nearly monodispersed hC-dots with a diameter in a range: 5-30nm as confirmed by high resolution transmission electron microscopy (HRTEM). The involvement of various functional groups was confirmed by Fourier transform infra-red (FTIR) spectroscopy. These hC-dots were incubated with breast cancer stem cells in order to check the entry as well as biological imaging. The cells were analyzed using epifluorescent microscopy. hC-dots showed concentration dependent cytotoxicity (LD50: 50mg/ml) and could be used for bioimaging even at lower concentration (0.5mg/ml). hC-dots were found to be versatile agents for peeping inside the cells which could also be used for delivery of water insoluble chemotherapeutic agents to variety of solid tumors.
authors.affiliation: Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
authors.forename: Abou
authors.initials: A
authors.lastname: Talib
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan.
authors.forename: Sunil
authors.initials: S
authors.lastname: Pandey
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan.
authors.forename: Mukeshchand
authors.initials: M
authors.lastname: Thakur
authors.affiliation: Department of Chemistry, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 800, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan. Electronic address: hui@faculty.nsysu.edu.tw.
authors.forename: Hui-Fen
authors.initials: HF
authors.lastname: Wu
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D002244:Carbon / Q000737:chemistry*; D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008856:Microscopy, Fluorescence; D014411:Neoplastic Stem Cells; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D002244:Carbon
keywords: Biological imaging; Breast cancer stem cells; Carbon dots; Hydrophobic; Paraplast