title: An improved HPLC-DAD method for quantitative comparisons of triterpenes in Ganoderma lucidum and its five related species originating from Vietnam.
abstract: An HPLC-DAD method for the quality control of wild and cultivated Ganoderma lucidum (Linhzhi) and related species samples was developed and validated. The quantitative determination of G. lucidum and its related species using 14 triterpene constituents, including nine ganoderma acids (compounds 4-12), four alcohols (compounds 13-16), and one sterol (ergosterol, 17) were reported. The standard curves were linear over the concentration range of 7.5-180 µg/mL. The LOD and LOQ values for the analyses varied from 0.34 to 1.41 µg/mL and from 1.01 to 4.23 µg/mL, respectively. The percentage recovery of each reference compound was found to be from 97.09% to 100.79%, and the RSD (%) was less than 2.35%. The precision and accuracy ranged from 0.81%-3.20% and 95.38%-102.19% for intra-day, and from 0.43%-3.67% and 96.63%-103.09% for inter-day, respectively. The study disclosed in detail significant differences between the quantities of analyzed compounds in different samples. The total triterpenes in wild Linhzhi samples were significantly higher than in cultivated ones. The total constituent contents of the five related Linhzhi samples were considerably lower than that in the G. lucidum specimens, except for G. australe as its constituent content outweighed wild Linhzhi's content by 4:1.
authors.affiliation: National Institute of Medicinal Materials (NIMM), 3B Quangtrung, Hoankiem, Hanoi 100000, Vietnam. hado.nimms@gmail.com.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Ha
authors.affiliation: National Institute of Medicinal Materials (NIMM), 3B Quangtrung, Hoankiem, Hanoi 100000, Vietnam. vloandkh@gmail.com.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Loan
authors.affiliation: Faculty of Chemistry, University of Science, Vietnam National University-HoChiMinh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City 227-01, Vietnam. tmhung801018@gmail.com.
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.affiliation: National Institute of Medicinal Materials (NIMM), 3B Quangtrung, Hoankiem, Hanoi 100000, Vietnam. levungochan@gmail.com.
authors.forename: Le Vu Ngoc
authors.initials: le VN
authors.lastname: Han
authors.affiliation: National Institute of Medicinal Materials (NIMM), 3B Quangtrung, Hoankiem, Hanoi 100000, Vietnam. khoi_nguyenminh@yahoo.co.uk.
authors.forename: Nguyen Minh
authors.initials: NM
authors.lastname: Khoi
authors.affiliation: National Institute of Medicinal Materials (NIMM), 3B Quangtrung, Hoankiem, Hanoi 100000, Vietnam. levietdung75imm@yahoo.com.
authors.forename: Le Viet
authors.initials: le V
authors.lastname: Dung
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeungbuk 712-702, Korea. bsmin@cu.ac.kr.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.affiliation: Department of Biology, University of Taynguyen, 567 Le Duan, Dac Lac City 630000, Vietnam. nguyendhtn@gmail.com.
authors.forename: Nguyen Phuong Dai
authors.initials: NP
authors.lastname: Nguyen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032902:Ganoderma / Q000737:chemistry*; D012044:Regression Analysis; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D014744:Vietnam
chemical_list: D014315:Triterpenes