title: Biomolecular Interaction Analysis of Gestrinone-anti-Gestrinone Using Arrays of High Aspect Ratio SU-8 Nanopillars.
abstract: In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. fraorhi1@upvnet.upv.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Ortega
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. mbpolo@upvnet.upv.es.
authors.forename: María-José
authors.initials: MJ
authors.lastname: Bañuls
authors.affiliation: Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. fj_sanza@yahoo.es.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Sanza
authors.affiliation: Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. rafael.casquel@upm.es.
authors.forename: Rafael
authors.initials: R
authors.lastname: Casquel
authors.affiliation: Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. mariafe.laguna@upm.es.
authors.forename: María Fe
authors.initials: MF
authors.lastname: Laguna
authors.affiliation: Centro Láser, Universidad Politécnica de Madrid, Campus Sur, Madrid 28031, Spain. m.holgado@upm.es.
authors.forename: Miguel
authors.initials: M
authors.lastname: Holgado
authors.affiliation: Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain. dlromero@die.upm.es.
authors.forename: David
authors.initials: D
authors.lastname: López-Romero
authors.affiliation: Instituto de Sistemas Optoelectrónicos y Microtecnología, Universidad Politécnica de Madrid, ETSI de Telecomunicación, Ciudad Universitaria s/n, Madrid 28040, Spain. carlos.angulo.barrios@upm.es.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Barrios
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. amaquieira@qim.upv.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
authors.affiliation: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, Valencia 46022, Spain. rpuchades@qim.upv.es.
authors.forename: Rosa
authors.initials: R
authors.lastname: Puchades