title: Changes in fibrillin-1 expression, elastin expression and skin surface texture at sites of cultured epithelial autograft transplantation onto wounds from burn scar excision.
abstract: This study investigated the recovery process during which grafted cultured epithelium generated skin elasticity and skin surface microarchitecture. The subjects were 18 patients whose burn scars were excised at a depth not exposing the fat layer and who subsequently received cultured epithelial autografts. A total of 24 samples were obtained from the grafted sites: 6 samples within 6 weeks (stage 1), 5 samples after 6 weeks and within 6 months (stage 2), 6 samples after 6 months and within 18 months (stage 3) and 7 samples beyond 18 months (stage 4) of transplantation. These samples were evaluated by taking replicas of skin surface, and histological changes of fibrillin-1 and elastin. The expression patterns were classified using a grading scale. The grade of skin surface texture was significantly higher at stage 3 and marginally significantly higher at stage 4 compared with stage 1. The grade of fibrillin-1 was marginally significantly higher at stage 3 and significantly higher at stage 4 compared with stage 1. The grade of elastin was marginally significantly higher at stage 4 compared with stage 1. These results showed that it is important for patients to have skin care and avoid external forces for at least 18 months after transplantation.
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Kadoya
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Amano
authors.affiliation: Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Inomata
authors.affiliation: Shiseido Research Center, Yokohama, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsunenaga
authors.affiliation: Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Kumagai
authors.affiliation: Department of Plastic and Reconstructive Surgery, St. Marianna University School of Medicine, Kawasaki, Japan. k4matsu@marianna-u.ac.jp.|Department of Plastic and Reconstructive Surgery, Kawasaki Municipal Tama Hospital, Kawasaki, Japan. k4matsu@marianna-u.ac.jp.
authors.forename: Kyoichi
authors.initials: K
authors.lastname: Matsuzaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002056:Burns / Q000628:therapy*; D002478:Cells, Cultured; D002921:Cicatrix / Q000473:pathology*; D004548:Elasticity / Q000502:physiology; D004549:Elastin / Q000502:physiology*; D004848:Epithelium / Q000254:growth & development*; D005260:Female; D000071838:Fibrillin-1 / Q000502:physiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012867:Skin / Q000473:pathology; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing / Q000502:physiology*; D055815:Young Adult
chemical_list: D000071838:Fibrillin-1; D004549:Elastin
keywords: Burn scar; Cultured epithelium; Elasticity; Skin care; Wound