title: Methyl-CpG-binding domain protein MBD7 is required for active DNA demethylation in Arabidopsis.
abstract: Although researchers have established that DNA methylation and active demethylation are dynamically regulated in plant cells, the molecular mechanism for the regulation of active DNA demethylation is not well understood. By using an Arabidopsis (Arabidopsis thaliana) line expressing the Promoter RESPONSIVE TO DEHYDRATION 29A:LUCIFERASE (ProRD29A:LUC) and Promoter cauliflower mosaic virus 35S:NEOMYCIN PHOSPHOTRANSFERASE II (Pro35S:NPTII) transgenes, we isolated an mbd7 (for methyl-CpG-binding domain protein7) mutant. The mbd7 mutation causes an inactivation of the Pro35S:NPTII transgene but does not affect the expression of the ProRD29A:LUC transgene. The silencing of the Pro35S:NPTII reporter gene is associated with DNA hypermethylation of the reporter gene. MBD7 interacts physically with REPRESSOR OF SILENCING5/INCREASED DNA METHYLATION2, a protein in the small heat shock protein family. MBD7 prefers to target the genomic loci with high densities of DNA methylation around chromocenters. The Gypsy-type long terminal repeat retrotransposons mainly distributed around chromocenters are most affected by mbd7 in all transposons. Our results suggest that MBD7 is required for active DNA demethylation and antisilencing of the genomic loci with high densities of DNA methylation in Arabidopsis.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Chunlei
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Dan
authors.initials: D
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Shaojun
authors.initials: S
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Xinjian
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Zhaobo
authors.initials: Z
authors.lastname: Lang
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Lai
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.).
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences (C.W., D.J., Y.Z., X.L., Z.G.), State Key Laboratory of Agrobiotechnology (X.D., J.L.), and China National Maize Improvement Center, Department of Plant Genetics and Breeding (X.D., J.L.), China Agricultural University, Beijing 100193, China;Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana 47906 (S.X., Z.L., J.-K.Z.);National Institute of Biological Sciences, Beijing 102206, China (X.H.); andShanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China (J.-K.Z.) gongzz@cau.edu.cn.
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Gong
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D001483:Base Sequence; D003001:Cloning, Molecular; D019175:DNA Methylation / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018745:Genome, Plant; D008954:Models, Biological; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C525054:MBD7 protein, Arabidopsis