title: Magnetic purification of curcumin from Curcuma longa rhizome by novel naked maghemite nanoparticles.
abstract: Naked maghemite nanoparticles, namely, surface active maghemite nanoparticles (SAMNs), characterized by a diameter of about 10 nm, possessing peculiar colloidal stability, surface chemistry, and superparamagnetism, present fundamental requisites for the development of effective magnetic purification processes for biomolecules in complex matrices. Polyphenolic molecules presenting functionalities with different proclivities toward iron chelation were studied as probes for testing SAMN suitability for magnetic purification. Thus, the binding efficiency and reversibility on SAMNs of phenolic compounds of interest in the pharmaceutical and food industries, namely, catechin, tyrosine, hydroxytyrosine, ferulic acid, coumaric acid, rosmarinic acid, naringenin, curcumin, and cyanidin-3-glucoside, were evaluated. Curcumin emerged as an elective compound, suitable for magnetic purification by SAMNs from complex matrices. A combination of curcumin, demethoxycurcumin, and bis-demethoxycurcumin was recovered by a single magnetic purification step from extracts of Curcuma longa rhizomes, with a purity >98% and a purification yield of 45%, curcumin being >80% of the total purified curcuminoids.
authors.affiliation: Department of Comparative Biomedicine and Food Science, University of Padua , Legnaro, 35020 PD, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Magro
authors.forename: Rene
authors.initials: R
authors.lastname: Campos
authors.forename: Davide
authors.initials: D
authors.lastname: Baratella
authors.forename: Maria Izabela
authors.initials: MI
authors.lastname: Ferreira
authors.forename: Emanuela
authors.initials: E
authors.lastname: Bonaiuto
authors.forename: Vittorino
authors.initials: V
authors.lastname: Corraducci
authors.forename: Maíra Rodrigues
authors.initials: MR
authors.lastname: Uliana
authors.forename: Giuseppina Pace Pereira
authors.initials: GP
authors.lastname: Lima
authors.forename: Silvia
authors.initials: S
authors.lastname: Santagata
authors.forename: Paolo
authors.initials: P
authors.lastname: Sambo
authors.forename: Fabio
authors.initials: F
authors.lastname: Vianello
mesh_terms: D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000302:isolation & purification*; D005290:Ferric Compounds; D060328:Magnetic Phenomena; D053758:Nanoparticles; D027343:Rhizome / Q000737:chemistry*; D015204:Spectroscopy, Mossbauer; D013501:Surface-Active Agents
chemical_list: D005290:Ferric Compounds; D013501:Surface-Active Agents; C000499:ferric oxide; D003474:Curcumin
keywords: Curcuma longa; curcumin; curcuminoids; magnetic nanoparticles; magnetic purification; polyphenols