title: Screening of bioconstituents and in vitro cytotoxicity of Clematis gouriana leaves.
abstract: Clematis gouriana (Ranunculaceae), a perennial herb, is used by the local inhabitants of the western Himalayan region for its medicinal properties. Major bioconstituents of C. gouriana leaves using different solvent extracts were obtained and analysed. The results revealed promising contents of phenolics (from 18.19 ± 0.10 to 22.17 ± 0.10 mg g(-1)) as gallic acid and flavonoids (from 2.83 ± 0.01 to 6.52 ± 0.08 mg g(-1)) as quercetin equivalent in different extracts. Aqueous acetone extract showed higher antioxidant activity with IC50 value of 129.11 and 25.35 μg mL(-1) against DPPH and ABTS free radicals, respectively. Antioxidant yield ranged from 16.87 ± 0.27 to 24.48 ± 0.13 mg g(-1) of Trolox equivalent in different extracts as measured by the FRAP assay. Furthermore, ethylacetate extract exhibited strong in vitro cytotoxicity against Chinese hamster ovary and glioma cell lines. Proximate composition (proteins, fats, ash and minerals) of C. gouriana leaves was also assessed. Results demonstrated the potential of C. gouriana bioconstituents as nutraceuticals.
authors.affiliation: a CSIR-Institute of Himalayan Bioresource Technology, Academy of Scientific and Innovative Research , Palampur 176061 , Himachal Pradesh , India.|b Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Shalika
authors.initials: S
authors.lastname: Rana
authors.affiliation: a CSIR-Institute of Himalayan Bioresource Technology, Academy of Scientific and Innovative Research , Palampur 176061 , Himachal Pradesh , India.|b Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Rawat
authors.affiliation: b Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Madhavi
authors.initials: M
authors.lastname: Mahendru
authors.affiliation: a CSIR-Institute of Himalayan Bioresource Technology, Academy of Scientific and Innovative Research , Palampur 176061 , Himachal Pradesh , India.|b Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Yogendra
authors.initials: Y
authors.lastname: Padwad
authors.affiliation: c Division of Hill Area Tea Science, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Yogesh B
authors.initials: YB
authors.lastname: Pakade
authors.affiliation: d Division of Biodiversity, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Brij
authors.initials: B
authors.lastname: Lal
authors.affiliation: a CSIR-Institute of Himalayan Bioresource Technology, Academy of Scientific and Innovative Research , Palampur 176061 , Himachal Pradesh , India.|b Food and Nutraceutical Lab, Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology , Palampur 176061 , Himachal Pradesh , India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Bhushan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D016466:CHO Cells; D045744:Cell Line, Tumor; D039324:Clematis / Q000737:chemistry*; D003412:Cricetulus; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D020128:Inhibitory Concentration 50; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts
keywords: Clematis gouriana; antioxidants; cytotoxicity; phenolics; protein