title: Investigation of the functional properties and subcellular localization of alpha human and rainbow trout estrogen receptors within a unique yeast cellular context.
abstract: Estrogens are steroid hormones that play a pivotal role in growth, differentiation and function of reproductive and non-reproductive tissues, mediated through estrogen receptors (ERs). Estrogens are involved in different genomic and non-genomic cell signaling pathways which involve well-defined subcellular ER localizations. Thus, ER activity results from complex interplays between intrinsic binding properties and specific subcellular localization. Since these two factors are deeply intricate, we carried out, in a unique yeast cell context, a comparative study to better understand structure/function/subcellular distribution relationships. This was carried out by comparing two ERs: the human ER α subtype (hERα) and the short form of the α isoform of the rainbow trout ER (rtERαS). Their distinct binding properties to agonist and antagonist ligands and subcellular localizations were characterized in Saccharomyces cerevisiae yeast cells. An unexpected partial agonistic effect of ICI 182-780 was observed for rtERαS. Concomitant to distinct binding properties, distinct subcellular localizations were observed before and after ligand stimulation. Due to the unique cell context, the link between ERs intrinsic binding properties and subcellular localizations is partly unveiled and issues are hypothesized based on the role of cytoplasmic transient complexes which play a role in the ER cytoplasmic/nuclear partition, which in turn is critical for the recruitment of co-regulators in the nucleus.
authors.affiliation: Laboratoire d'Ingénierie des Matériaux de Bretagne, Université de Bretagne-Sud, CER Yves Coppens, BP573, 56017 Vannes Cedex, France.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Le Grand
authors.affiliation: Molecular Imaging and NanoBioTechnology, UMR 5248 CBMN, CNRS-Université Bordeaux 1-ENITAB, IECB, 2 rue Robert Escarpit, 33607 Pessac, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Bouter
authors.affiliation: CNRS/Université de Rennes 1, Institut de Génétique & Développement de Rennes, UMR 6290, 2 Ave. Prof. Léon Bernard, CS 34317, 35043 Rennes Cedex, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Couturier
authors.affiliation: CNRS/UPMC Université Paris 06, UMR 8227 LBI2M, Traduction, Cycle Cellulaire et Développement, Station Biologique, CS 90074, 29688 Roscoff Cedex, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Mulner-Lorillon
authors.affiliation: CNRS/Université de Rennes 1, Institut de Génétique & Développement de Rennes, UMR 6290, 2 Ave. Prof. Léon Bernard, CS 34317, 35043 Rennes Cedex, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Le Goff
authors.affiliation: CNRS/Université de Rennes 1, Institut de Génétique & Développement de Rennes, UMR 6290, 2 Ave. Prof. Léon Bernard, CS 34317, 35043 Rennes Cedex, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Chesnel
authors.affiliation: Laboratoire d'Ingénierie des Matériaux de Bretagne, Université de Bretagne-Sud, CER Yves Coppens, BP573, 56017 Vannes Cedex, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Sire
authors.affiliation: Laboratoire d'Ingénierie des Matériaux de Bretagne, Université de Bretagne-Sud, CER Yves Coppens, BP573, 56017 Vannes Cedex, France. Electronic address: letilly@univ-ubs.fr.
authors.forename: Véronique
authors.initials: V
authors.lastname: Le Tilly
mesh_terms: D000818:Animals; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D004967:Estrogens / Q000494:pharmacology; D029941:Fish Proteins / Q000819:agonists / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D017686:Oncorhynchus mykiss / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects; D014170:Transformation, Genetic
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004967:Estrogens; D029941:Fish Proteins; D008024:Ligands
keywords: Confocal fluorescence microscopy; Estrogen receptor; Ligand binding; Subcellular localization; Transcriptional activity