title: [Bone mesenchymal stem cell transplantation for acute myocardial infarction: in vivo tracing with multi-modality molecular imaging].
abstract: OBJECTIVE
  To investigate the feasibility of tracking bone mesenchymal stem cell (BMSCs) dual- labeled with polyethylenimine 2k-superparamagnetic iron oxide (PEI2k-SPIO) and Luciferase transplantation for acute myocardial infarction in vivo by using magnetic resonance imaging (MRI) and fluorescence imaging.
  
  
  METHODS
  BMSCs/Luciferase was incubated with culture medium containing PEI2k-SPIO for 24 h. Prussian-blue staining and MTT were used to assess the efficacy and safety of labeling with PEI2k-SPIO. Guided with echocardiography, the dual-labeled BMSCs were injected into the margin of infarction myocardium. MRI and fluorescence imaging were performed to monitor the cells in vivo at different times (1,2,3,7 d).
  
  
  RESULTS
  As demonstrated by MTT, there was no significant difference in survival rate between the labeled and unlabeled cells (P>0. 05). Within a week after transplantation, all PEI2k-SPIO-labeled BMSCs showed a significant decreased signal on MRI. Dual-labeled BMSCs were detected bioluminescence with fluorescence imaging, but disappeared after one week.
  
  
  CONCLUSIONS
  Multi- modality imaging can not only trace the location of labeled BMSCs but also demonstrate the survival of labeled BMSCs in vivo.
authors.affiliation: Department of Radiology, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.forename: Hui-zhu
authors.initials: HZ
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Xia
authors.forename: Ying-kun
authors.initials: YK
authors.lastname: Guo
authors.forename: Jiang-long
authors.initials: JL
authors.lastname: Hou
authors.lastname: Zhang Li-zhi
authors.forename: Zhen-lin
authors.initials: ZL
authors.lastname: Li
authors.forename: Hua
authors.initials: H
authors.lastname: Ai
authors.forename: Gang
authors.initials: G
authors.lastname: Ning
authors.forename: Fa-bao
authors.initials: FB
authors.lastname: Gao
mesh_terms: D000818:Animals; D003911:Dextrans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D057054:Molecular Imaging; D009203:Myocardial Infarction / Q000628:therapy*; D011094:Polyethyleneimine
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D011094:Polyethyleneimine; C065507:ferumoxides