title: [Anti-tumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line in vitro].
abstract: OBJECTIVE
  To explore the antitumor effects of DDP-PLLA-CNTs on human cholangiocarcinoma cell line.
  
  
  METHODS
  DDP-PLLA-CNTs were prepared with the method of ultrasound emulsification. The morphology of DDP-PLLA-CNTs was determined by scanning electron microscope (SEM). And its drug loading and drug release curve in vitro was detected by UV-Vis-NIR spectrophotometer. CCK8 was used to test the cytotoxic effects of DDP-PLLA-CNTs at different concentrations on QBC939 cell proliferation.Flow cytometry was employed to measure the changes of apoptotic rate.
  
  
  RESULTS
  With excellent controlled-release characteristic of in vitro drug release, DDP-PLLA-CNTs inhibited the proliferation and significantly increased the apoptotic rate of QBC939 cell line.
  
  
  CONCLUSIONS
  DDP-PLLA-CNTs have drug sustained-release characteristics and can significantly inhibit the proliferation of QBC939 cell line.
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Qichen
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Weng
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Weibin
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Dong
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department & Laboratory of General Surgery, Affiliated Xinhua Hospital, Shanghai Jiaotong University, School of Medicine; Institute of Biliary Tract Disease, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. Email: laoniulyb@163.com.
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001650:Bile Duct Neoplasms; D001653:Bile Ducts, Intrahepatic; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018281:Cholangiocarcinoma; D005663:Furans; D006801:Humans; D019344:Lactic Acid; D037742:Nanotubes, Carbon; D011091:Polyesters; D011108:Polymers; D011753:Pyrones
chemical_list: C458502:2,6-dimethyldifuro-8-pyrone; D005663:Furans; D037742:Nanotubes, Carbon; D011091:Polyesters; D011108:Polymers; D011753:Pyrones; D019344:Lactic Acid; C033616:poly(lactide)