title: Strain-specific interaction of a GII.10 Norovirus with HBGAs.
abstract: Noroviruses (NoVs), an important cause of gastroenteritis in humans, recognize human histo-blood group antigens (HBGAs) as receptors. The crystal structures of the protruding (P) domain of a GII.10 NoV (Vietnam 026) in complex with various HBGA oligosaccharides were elucidated. However, the HBGA binding profile of this virus remains unknown. In this study, we determined the saliva and oligosaccharide binding profiles of this virus and the roles of amino acids that are involved in HBGA binding. Our data showed that Vietnam 026 bound to all ABO secretor and non-secretor saliva with clear signals detected by monoclonal antibodies against H3, H1, Le(y), Le(a) and sialyl Le(a). Mutagenesis study confirmed the binding site determined by the crystallography study, in which single mutations wiped out the binding function. We also identified amino acids surrounding the central binding pocket that may participate in the binding by affecting the HBGA binding specificity of the GII.10 NoV.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road Street, Chang-ping District, Beijing 102206, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jin
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Tan
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA.
authors.forename: Ming
authors.initials: M
authors.lastname: Xia
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA.
authors.forename: Chao
authors.initials: C
authors.lastname: Wei
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA.
authors.forename: Pengwei
authors.initials: P
authors.lastname: Huang
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA.
authors.forename: Leyi
authors.initials: L
authors.lastname: Wang
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA.
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhong
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, 155 Changbai Road Street, Chang-ping District, Beijing 102206, China. Electronic address: zhaojund@126.com.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Duan
authors.affiliation: Divisions of Infectious Diseases, Cincinnati Children׳s Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, USA; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Electronic address: jason.jiang@cchmc.org.
authors.forename: Xi
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000595:Amino Acid Sequence; D001789:Blood Group Antigens / Q000235:genetics / Q000378:metabolism*; D017250:Caliciviridae Infections / Q000378:metabolism* / Q000821:virology; D005759:Gastroenteritis / Q000378:metabolism* / Q000821:virology; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D029322:Norovirus / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D001789:Blood Group Antigens; D011991:Receptors, Virus; D017361:Viral Nonstructural Proteins
keywords: Acute gastroenteritis; Human histo-blood group antigens; Mutagenesis; Noroviruses; Oligosaccharide; Saliva