title: Investigation of methanogenic community structures in rural biogas digesters from different climatic regions in Yunnan, southwest China.
abstract: Understanding of the microbial community structures of the biogas digesters in different climatic regions can help improve the methane production in the fermentation process. The methanogenic archaeal diversity in four rural biogas digesters (BNA, JSA, LJA, and XGA) was investigated by a culture-independent rRNA approach in different climatic regions in Yunnan. Community structure composed of 711 clones in the all libraries. A total of 33 operational taxonomic units (OTUs) were detected, and major groups of methanogens were the orders Methanosarcinales and Methanomicrobiales. 63.2 % of all archaeal OTUs belong to the order Methanosarcinales which mostly contain acetotrophic methanogens. Methanomicrobiales (19.5 % in all OTUs) were detected in considerable number. Additionally, there were minor rates of uncultured archaea. The principal component analysis indicated that the genus Methanosaeta was mainly affected by the fermentation temperatures.
authors.affiliation: Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, China, dongminghua08@126.com.
authors.forename: Minghua
authors.initials: M
authors.lastname: Dong
authors.forename: Yan
authors.initials: Y
authors.lastname: Wu
authors.forename: Qiumin
authors.initials: Q
authors.lastname: Li
authors.forename: Guangliang
authors.initials: G
authors.lastname: Tian
authors.forename: Bin
authors.initials: B
authors.lastname: Yang
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Li
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiao
authors.forename: Fang
authors.initials: F
authors.lastname: Yin
authors.forename: Xingling
authors.initials: X
authors.lastname: Zhao
authors.forename: Wudi
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Cui
mesh_terms: D001105:Archaea / Q000145:classification* / Q000235:genetics; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D058448:Biota; D002681:China; D002980:Climate; D016000:Cluster Analysis; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012424:Rural Population; D017422:Sequence Analysis, DNA
chemical_list: D056804:Biofuels; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D008697:Methane