title: Enhanced expression of neurotrophic factors in the injured spinal cord through vaccination with myelin basic protein-derived peptide pulsed dendritic cells.
abstract: METHODS
  Vaccination of spinal cord injury (SCI) mice with myelin basic protein-derived peptide (A91) pulsed dendritic cells (DC) to enhance brain-derived neurotrophic factor and neurotrophin-3 (NT-3) expression in injured spinal cord.
  
  
  OBJECTIVE
  To investigate the effect of A91-pulsed DC (A91-DC) on expression of neurotrophic factor in injured spinal cord.
  
  
  BACKGROUND
  SCI leads to progressive secondary tissue degeneration, and no satisfactory treatment is currently available. Accumulating evidence indicates that administration of neurotrophic factors to injured spinal cord is partially successful at promoting nerve tissue repair. However, most of strategy can cause secondary injury and limiting their wide clinical application.
  
  
  METHODS
  Proliferation of T cells and the capability of CD4 T cells to secret neurotrophic factors were first measured in vitro to demonstrate the stimulus action of the A91-DC. In SCI mice model, enzyme-linked immunosorbent assay and immunofluorescence was employed to investigate the brain-derived neurotrophic factor and NT-3 expression in injured spinal cord. Furthermore, the neuroprotective effect of A91-DC in injured spinal cord was examined through histology measurement.
  
  
  RESULTS
  In this study, we demonstrated that A91-DC promoted the capability of T cells to secret neurotrophic factors and in the subacute phase of SCI. Moreover, vaccination with A91-DC enhanced the expression level of brain-derived neurotrophic factor and NT-3 and exerted neuroprotective effect in injured spinal cord.
  
  
  CONCLUSIONS
  The findings of study demonstrate that the therapeutic strategy of vaccination A91-DC is a potential minimally invasive approach that could provide strong neurotrophic factor support after SCI.
authors.affiliation: *Department of Orthopedics Surgery, Second Hospital, Harbin Medical University, Harbin, P.R. China †Department of Pathology and Center of Electron Microscope, School of Basic Medicine, Harbin Medical University, Harbin, P.R. China; and ‡Li Ka Shing Institute of Virology, University of Alberta, Edmonton, Canada.
authors.forename: Yufu
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Pengyu
authors.initials: P
authors.lastname: Kong
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.forename: Jinglong
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology*; D004195:Disease Models, Animal; D051379:Mice; D008807:Mice, Inbred BALB C; D004676:Myelin Basic Protein / Q000276:immunology*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D020933:Neurotrophin 3 / Q000378:metabolism*; D020127:Recovery of Function / Q000187:drug effects*; D013116:Spinal Cord / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D016896:Treatment Outcome; D014612:Vaccines / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D004676:Myelin Basic Protein; D018696:Neuroprotective Agents; D020933:Neurotrophin 3; D014612:Vaccines