title: Simultaneous ultrasound-assisted water extraction and β-cyclodextrin encapsulation of polyphenols from Mangifera indica stem bark in counteracting TNFα-induced endothelial dysfunction.
abstract: This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. β-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the β-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and β-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.
authors.affiliation: a Vascular and Endovascular Surgery Unit, Research Laboratory of Experimental and Clinical Vascular Biology, DISC , University of Genoa , IRCCS San Martino - IST, 16132 Genoa , Italy.
authors.forename: Marzia
authors.initials: M
authors.lastname: Mura
authors.forename: Daniela
authors.initials: D
authors.lastname: Palmieri
authors.forename: Davide
authors.initials: D
authors.lastname: Garella
authors.forename: Antonella
authors.initials: A
authors.lastname: Di Stilo
authors.forename: Patrizia
authors.initials: P
authors.lastname: Perego
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Cravotto
authors.forename: Domenico
authors.initials: D
authors.lastname: Palombo
mesh_terms: D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D031022:Mangifera / Q000737:chemistry*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D010766:Phosphorylation; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014465:Ultrasonics; D047392:beta-Cyclodextrins; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; C573420:ICAM1 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D059808:Polyphenols; D014409:Tumor Necrosis Factor-alpha; D047392:beta-Cyclodextrins; D018799:Intercellular Adhesion Molecule-1; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D048051:p38 Mitogen-Activated Protein Kinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; C031215:betadex
keywords: Mangifera indica; TNFα; endothelial dysfunction; oestrogen receptor; ultrasound-assisted extraction; β-cyclodextrin