title: MALD-TOF study of peptides from cryopreserved tissue fragments.
abstract: BACKGROUND
  Previously it was shown that the extracts of cryopreserved organ fragments, containing tissue specific peptides, stimulates the reparative regeneration. In order to define the mechanism of action, it is necessary to determine the peptidic composition of extracts.
  
  
  OBJECTIVE
  To examine molecular-mass distribution of peptides in the extracts of cryopreserved fragments of pigs' heart and spleen as well as piglets' heart and skin.
  
  
  METHODS
  The extracts were obtained from cryopreserved fragments of porcine organs. MALDI-ToF method was used to study the peptidic composition of the extracts.
  
  
  RESULTS
  The most intense mass spectra peaks, which may correspond to single peptidic molecules, were compared with Protein Knowledgebase (UniProtKB) data. A number of recorded peptides in the extracts expresses high biological activity.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine.
authors.forename: Liliia
authors.initials: L
authors.lastname: Rohoza
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine.
authors.forename: Iryna
authors.initials: I
authors.lastname: Bespalova
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine. sgalchenko@yandex.ua.
authors.forename: Sergiy
authors.initials: S
authors.lastname: Galchenko
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, 61015, Ukraine.
authors.forename: Boris
authors.initials: B
authors.lastname: Sandomirsky
mesh_terms: D000818:Animals; D015925:Cryopreservation; D009206:Myocardium / Q000737:chemistry*; D009926:Organ Preservation; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D012867:Skin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013154:Spleen / Q000737:chemistry*; D013552:Swine
chemical_list: D010455:Peptides