title: Genomic alterations on 8p21-p23 are the most frequent genetic events in stage I squamous cell carcinoma of the lung.
abstract: Genetic alterations in the early stages of cancer have a close correlation with tumor initiation and potentially activate downstream pathways implicated in tumor progression; however, the method of initiation in sporadic neoplasias is largely unknown. In this study, whole-genome microarray-comparative genomic hybridization was performed to identify the early genetic alterations that define the prognosis of patients with stage I squamous cell carcinoma (SCC) of the lung. The most striking finding was the high frequency of copy number losses and hemizygous deletions on chromosome 8p, which occurred in 94.7% (18/19) and 63.2% (12/19) of the cases, respectively, with a delineated minimal common region of 8p21.1-p23.3. More specifically, three loci of homozygous deletions at 8p23.1 were noted in 21.1% (4/19) of the cases. This region contains the following possible target genes, which have previously not been implicated to play a pathogenic role in stage I SCCs: MSRA,MFHAS1,CLDN23,DEFB106A,DEFB105A,LOC441316,FAM90A7PandLOC441318. These findings indicate that genetic alterations on chromosome 8p may be the first step in the initiation of genomic instability in early SCCs, and the newly identified genes in the 8p23.1 chromosomal region might be of interest for the study of the pathophysiology of stage I SCC, as potential targets for therapeutic measures.
authors.affiliation: Department of Biomedical Laboratory Science, Korea Nazarene University, Cheonan 330-718, Republic of Korea.
authors.forename: Jiun
authors.initials: J
authors.lastname: Kang
keywords: copy number loss; homozygous deletion; microarray-comparative genomic hybridization; squamous cell carcinoma of the lung; tumor suppressor genes