title: Super-resolution imaging for cell biologists: concepts, applications, current challenges and developments.
abstract: The recent 2014 Nobel Prize in chemistry honored an era of discoveries and technical advancements in the field of super-resolution microscopy. However, the applications of diffraction-unlimited imaging in biology have a long road ahead and persistently engage scientists with new challenges. Some of the bottlenecks that restrain the dissemination of super-resolution techniques are tangible, and include the limited performance of affinity probes and the yet not capillary diffusion of imaging setups. Likewise, super-resolution microscopy has introduced new paradigms in the design of projects that require imaging with nanometer-resolution and in the interpretation of biological images. Besides structural or morphological characterization, super-resolution imaging is quickly expanding towards interaction mapping, multiple target detection and live imaging. Here we review the recent progress of biologists employing super-resolution imaging, some pitfalls, implications and new trends, with the purpose of animating the field and spurring future developments.
authors.affiliation: STED Microscopy Group, European Neuroscience Institute, Göttingen, Germany; Department of Neuro- and Sensory-physiology, University of Göttingen, Göttingen, Germany.
authors.forename: Eugenio F
authors.initials: EF
authors.lastname: Fornasiero
authors.forename: Felipe
authors.initials: F
authors.lastname: Opazo
mesh_terms: D008853:Microscopy / Q000379:methods*
keywords: PALM; SIM; STED; STORM; affinity-probes; cell imaging; sub-diffraction imaging