title: Facile synthesis of hairy core-shell structured magnetic polymer submicrospheres and their adsorption of bovine serum albumin.
abstract: Highly magnetic polymer submicrospheres with a hairy core-shell structure were facilely synthesized by combining distillation-precipitation polymerization (DPP) with subsequent surface-initiated atom transfer radical polymerization (SI-ATRP), and then investigated for protein adsorption. A robust polymer shell consisting of poly(divinylbenzene-co-chloromethylstyrene) (P(DVB-co-CMS)) was coated on superparamagnetic submicrometer-sized magnetite colloid nanocrystal clusters (MCNCs) via DPP. With the benzyl chloride groups on the shell as initiator, poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) hairs were grafted by SI-ATRP approach. The resulting hairy core-shell structured Fe3O4@ P(DVB-co-CMS)-PDMAEMA microspheres showed pH- and temperature-sensitivity, and high-magnetization. The composite microspheres were further investigated for adsorption of a typical acidic protein, i.e. bovine serum albumin (BSA). They exhibited a high binding capacity up to over 660 mg/g (corresponding to 158 DMAEMA monomer units cooperating for binding one BSA molecule) and could rapidly reach binding equilibrium within 5 min. Moreover, the adsorption of BSA was found to be remarkably dependent on the pH and salt concentration of the protein solutions, and the bound protein could be quantitatively desorbed by washing with a medium with lowered pH or raised salt concentration.
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer, Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Yan
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer, Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Kong
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer, Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China.
authors.forename: Chongchong
authors.initials: C
authors.lastname: Yang
authors.affiliation: Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer, Chemistry Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Tianjin 300071, China. Electronic address: gqfu@nankai.edu.cn.
authors.forename: Guoqi
authors.initials: G
authors.lastname: Fu
mesh_terms: D000327:Adsorption; D000818:Animals; D001555:Benzene Derivatives / Q000138:chemical synthesis / Q000737:chemistry*; D002417:Cattle; D011232:Chemical Precipitation; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D060328:Magnetic Phenomena; D059346:Magnets / Q000737:chemistry*; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D008863:Microspheres; D009757:Nylons / Q000138:chemical synthesis / Q000737:chemistry*; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013343:Styrenes / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D001555:Benzene Derivatives; D008689:Methacrylates; D009757:Nylons; D013343:Styrenes; C407037:poly(2-(dimethylamino)ethyl methacrylate); C027142:vinyltoluene; D012710:Serum Albumin, Bovine; D052203:Ferrosoferric Oxide
keywords: Distillation–precipitation polymerization; Hairy core–shell structure; Magnetic polymer microspheres; Protein adsorption; Surface-initiated atom transfer radical polymerization