title: Physico-chemical characterization and pharmacological evaluation of sulfated polysaccharides from three species of Mediterranean brown algae of the genus Cystoseira.
abstract: BACKGROUND
  Seaweed polysaccharides are highly active natural substances having valuable applications. The present study was conducted to characterize the physico-chemical properties of sulphated polysaccharides from three Mediterranean brown seaweeds (Cystoseira sedoides, Cystoseira compressa and Cystoseira crinita) and to evaluate their anti-radical, anti-inflammatory and gastroprotective activities.
  
  
  METHODS
  The different rates of neutral sugars, uronic acids, L-fucose and sulphate content were determined by colorimetric techniques. The different macromolecular characteristics of isolated fucoidans were identified by size exclusion chromatography equipped with a triple detection: multiangle light scattering, viscometer and differential refractive index detectors, (SEC/MALS/VD/DRI). Anti-inflammatory activity was evaluated, using the carrageenan-induced rat paw edema test in comparison to the references drugs Acetylsalicylate of Lysine and Diclofenac. The gastroprotective activity was determined using HCl/EtOH induced gastric ulcers in rats and to examine the antioxidant effect of fucoidans in the three species, the free radical scavenging activity was determined using 1,1-diphenyl-2-picrylhydrazyl.
  
  
  RESULTS
  The pharmacological evaluation of the isolated fucoidans for their anti-inflammatory, and their gastroprotective effect established that these products from C. sedoides, C. compressa and C. crinita exhibited a significant anti-inflammatory activity at a dose of 50 mg/kg, i.p; the percentages of inhibition of the oedema were 51%, 57% and 58% respectively. And, at the same dose, these fucoidans from C. sedoides and C. compressa showed a significant decrease of the intensity of gastric mucosal damages compared to a control group by 68%, whereas, the fucoidan from C. crinita produced a less gastroprotective effect. Furthermore, the isolated fucoidans exhibited a radical scavenging activity.
  
  
  CONCLUSIONS
  The comparative study of fucoidans isolated from three species of the genus Cystoseira showed that they have similar chemicals properties and relatives anti-radical, anti-inflammatory and gastroprotective activities which are found to be promising.
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019, Monastir, Tunisia. hadjammarhiba@gmail.com.
authors.forename: Hiba
authors.initials: H
authors.lastname: Hadj Ammar
authors.affiliation: Laboratoire de développement chimique, galénique et pharmacologique des médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000, Monastir, Tunisia. sirinelajili@gmail.com.
authors.forename: Sirine
authors.initials: S
authors.lastname: Lajili
authors.affiliation: Institut National des Sciences et Techniques de la Mer (INSTM), Salambôo, Tunis, Tunisia. rafik.bensaid@instm.rnrt.tn.
authors.forename: Rafik
authors.initials: R
authors.lastname: Ben Said
authors.affiliation: Université de la Normandie, Laboratoire Polymères Biopolymères Surfaces, UMR 6270 CNRS Université de Rouen, FRE 3101 CNRS, 76821, Mont Saint Aignan, France. didier.lecerf@univ-rouen.fr.
authors.forename: Didier
authors.initials: D
authors.lastname: Le Cerf
authors.affiliation: Laboratoire de développement chimique, galénique et pharmacologique des médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000, Monastir, Tunisia. ursambouraoui@yahoo.fr.
authors.forename: Abderrahman
authors.initials: A
authors.lastname: Bouraoui
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019, Monastir, Tunisia. hatemmajdoub2002@yahoo.fr.
authors.forename: Hatem
authors.initials: H
authors.lastname: Majdoub
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification; D002351:Carrageenan; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D000431:Ethanol; D005260:Female; D006851:Hydrochloric Acid; D008297:Male; D000459:Phaeophyceae / Q000737:chemistry* / Q000145:classification; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D011134:Polysaccharides; D000431:Ethanol; D002351:Carrageenan; C007789:fucoidan; D006851:Hydrochloric Acid