title: Effects of the JWA gene in the regulation of human breast cancer cells.
abstract: The present study aimed to investigate whether the JWA gene can regulate the proliferation, migration and invasion of human breast cancer cells through the MAPK signaling pathway. The role of JWA in proliferation, migration, invasion and apoptosis was investigated in the MDA‑MB‑231 human breast cancer cell line. Following transfection with JWA‑small interfering (si)RNA, the effect of JWA on apoptosis was assessed by Western blot analysis, proliferation was determined using Transwell chambers and cell migration and invasion were analyzed by transwell assay. The expression levels of extracellular signal‑regulated kinase (ERK) 1/2, CSBP/RK/Mpk2 kinase (p38) and c‑Jun N‑terminal kinase (JNK) were detected using Western blot analysis in the siRNA and control groups. The expression of JWA in the breast cancer cells was significantly lower compared with the normal breast cells. Downregulation of JWA protein levels reduced the apoptosis and enhanced proliferation, migration and invasion of the MDA‑MB‑231 cells in vitro. The results of the Western blot analysis demonstrated that, compared with the control groups, the expression levels of phosphorylated (p‑)p38 decreased significantly in the JWA siRNA group. No significant changes were observed in the expression levels of p‑ERK1/2 or p‑JNK. Therefore, the JWA gene may regulate human breast cancer cells through the MAPK signaling pathway using different types of regulation.
authors.affiliation: Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Jiake
authors.initials: J
authors.lastname: Feng
authors.affiliation: Department of Critical Care Medicine, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Oncology, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Weiliang
authors.initials: W
authors.lastname: Ding
authors.affiliation: Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of General Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiac and Thoracic Surgery, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Central Laboratory, The Affiliated Yixing Hospital of Jiangsu University, Yixing, Jiangsu 214200, P.R. China.
authors.forename: Tieliang
authors.initials: T
authors.lastname: Ma
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006360:Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D020935:MAP Kinase Signaling System; D042361:Mammary Glands, Human / Q000378:metabolism; D026901:Membrane Transport Proteins; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: C484566:ARL6IP5 protein, human; D006360:Heat-Shock Proteins; D047908:Intracellular Signaling Peptides and Proteins; D026901:Membrane Transport Proteins; D034741:RNA, Small Interfering