title: A synthetic biology approach for the transformation of l-α-amino acids to the corresponding enantiopure (R)- or (S)-α-hydroxy acids.
abstract: Combinatorial assembly and variation of promoters on a single expression plasmid allowed the balance of the catalytic steps of a three enzyme (l-AAD, HIC, FDH) cascade in E. coli. The designer cell catalyst quantitatively transformed l-amino acids to the corresponding optically pure (R)- and (S)-α-hydroxy acids at up to 200 mM substrate concentration.
authors.affiliation: Austrian Centre of Industrial Biotechnology (ACIB), Petersgasse 14, 8010 Graz, Austria. birgit.wiltschi@acib.at.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Gourinchas
authors.forename: Eduardo
authors.initials: E
authors.lastname: Busto
authors.forename: Manuela
authors.initials: M
authors.lastname: Killinger
authors.forename: Nina
authors.initials: N
authors.lastname: Richter
authors.forename: Birgit
authors.initials: B
authors.lastname: Wiltschi
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D055162:Biocatalysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006880:Hydroxy Acids / Q000737:chemistry* / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D013237:Stereoisomerism; D058615:Synthetic Biology
chemical_list: D000596:Amino Acids; D006880:Hydroxy Acids