title: Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta.
abstract: Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P&lt;0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins.
authors.affiliation: Polytechnic University of Marche. giovorsini@yahoo.com.
authors.forename: G
authors.initials: G
authors.lastname: Orsini
authors.forename: A
authors.initials: A
authors.lastname: Majorana
authors.forename: A
authors.initials: A
authors.lastname: Mazzoni
authors.forename: A
authors.initials: A
authors.lastname: Putignano
authors.forename: M
authors.initials: M
authors.lastname: Falconi
authors.forename: A
authors.initials: A
authors.lastname: Polimeni
authors.forename: L
authors.initials: L
authors.lastname: Breschi
mesh_terms: D002648:Child; D024042:Collagen Type I / Q000378:metabolism*; D003804:Dentin / Q000378:metabolism / Q000473:pathology; D003811:Dentinogenesis Imperfecta / Q000378:metabolism / Q000473:pathology; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D053495:Osteopontin / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000378:metabolism / Q000473:pathology
chemical_list: D024042:Collagen Type I; C513309:DMP1 protein, human; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D053495:Osteopontin