title: Hydrobacter penzbergensis gen. nov., sp. nov., isolated from purified water.
abstract: A Gram-negative, oxidase- and catalase-positive bacterium, designated strain EM 4(T), which varied in shape from rod-shaped to curved or helical with frequently observed bulb-shaped protuberances, was isolated from purified water. 16S rRNA gene sequence analysis indicated that the novel strain belongs to the family Chitinophagaceae within the phylum Bacteroidetes; the closest relative among bacterial species with validly published names was determined to be Sediminibacterium salmoneum NBRC 103935(T), with 93.4 % sequence identity. The main fatty acids of strain EM 4(T) were iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, aminolipids, aminophospholipids and unknown lipids; the quinone system consisted of menaquinone MK-7. 16S rRNA gene sequence analysis and the polar lipid and fatty acid profiles suggest that the strain represents a novel genus and species, for which the name Hydrobacter penzbergensis gen. nov., sp. nov. is proposed. The type strain of Hydrobacter penzbergensis is strain EM 4(T) ( = DSM 25353(T) = CCUG 62278(T)).
authors.affiliation: Roche Diagnostics GmbH, Nonnenwald 2, 82377 Penzberg, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Eder
authors.affiliation: Ribocon GmbH, Fahrenheitstr. 1, 28359 Bremen, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Peplies
authors.affiliation: Department Biologie I, Biozentrum der LMU, Großhadernerstr. 2-4, 82152 Planegg-Martinsried, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Wanner
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7B, 38124 Braunschweig, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Frühling
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstr. 7B, 38124 Braunschweig, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Verbarg
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7