title: Synthesis and characterization of a novel chemically designed (Globo)3-DTPA-KLH antigen.
abstract: In recent years, many experiments have been conducted for the production and evaluation of anticancer glycoconjugated vaccines in developed countries and many achievements have been accomplished with Globo H derivatives. In the current experiment, a new chemically designed triplicate version of (Globo H)3-diethylenetriamine pentaacetic acid (DTPA)-KLH antigen was synthesized and characterized. Immunization with (Globo H)3-DTPA-KLH, a hexasaccharide that is a member of a family of antigenic carbohydrates that are highly expressed in various types of cancers conjugated with DTPA and KLH protein, induced a high level of antibody titer along with an elevated level of IL-4 in mice. Treatment of tumors with the collected sera from immunized mice decreased the tumor size in nude mice as well. None of the immunized mice illustrated any sign of tumor growth after injection of MCF-7 cells compared to the control animals. These findings, based on the newly presented structure of the Globo H antigen, lend exciting and promising evidence for clinical advancement in the development of a therapeutic vaccine in the future.
authors.affiliation: Department of Research and Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hajmohammadi
authors.affiliation: Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Seyed Davar
authors.initials: SD
authors.lastname: Siadat
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran ; Department of Virology, University of Ottawa, Ottawa, ON, Canada.
authors.forename: Masoud
authors.initials: M
authors.lastname: Ghorbani
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shafiee Ardestani
authors.affiliation: Department of Medical Genetics, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Teimourian
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Asgari
authors.affiliation: Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ahangari Cohan
authors.affiliation: Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Hajmohammadi
authors.affiliation: Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Hajmohammadi
authors.affiliation: Laboratory Animal Management of North Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Ramezan
authors.initials: R
authors.lastname: Behzadi
authors.affiliation: Department of Research and Development, Alhavi Pharmaceutical, Tehran, Iran.
authors.forename: Saied
authors.initials: S
authors.lastname: Rajab Nezhad
authors.affiliation: Pasteur Institute of Iran, Department of Animal Sciences, Karaj, Iran.
authors.forename: Nabiollah
authors.initials: N
authors.lastname: Namvar Asl
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry* / Q000276:immunology*; D019496:Cancer Vaccines / Q000737:chemistry* / Q000276:immunology; D015195:Drug Design; D005260:Female; D006433:Hemocyanins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D061986:MCF-7 Cells; D008325:Mammary Neoplasms, Experimental / Q000276:immunology* / Q000628:therapy*; D051379:Mice; D008819:Mice, Nude; D015394:Molecular Structure; D004369:Pentetic Acid / Q000737:chemistry* / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D015295:Antigens, Tumor-Associated, Carbohydrate; D019496:Cancer Vaccines; C472765:Globo-H; D004369:Pentetic Acid; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
keywords: (Globo H)3-DTPA-KLH; breast cancer; glycoconjugate vaccines