title: Correlation between radiographic area and immunolocation of MMP-2 and MMP-9 in unilocular radiographic lesions.
abstract: Unilocular bone cysts are the most common entities affecting the maxillofacial region. The mechanism of proliferation and expansion remains unclear. Metalloproteinases (MMPs) are associated to diverse pathological conditions. The aim of the present study was to correlate the radiographic aspect (area) and the presence of MMP-2 and MMP-9 in dentigerous cysts, radicular cysts and keratocystic odontogenic tumors. The radiographic area of each lesion was calculated using the mathematical formula of the ellipse area. All specimens were subjected to immunohistochemical analysis for these enzymes. The average radiographic area was 284.17 mm2, 235.81 mm2 and 381.81 mm2, respectively. Statistical analyses revealed no association between the immunoreactivity of MMPs and radiographic area of the lesions in all pathologies studied, except for MMP-2 and radicular cysts, for which smaller lesions had increased immunostaining for this enzyme. The results demonstrate that quantities of MMP-2 and MMP-9 are especially involved with dentigerous and radicular cysts in expansion, whereas these enzymes seem to be related to the biological behavior of keratocystic odontogenic tumors, indicating invasion and cell proliferation. Moreover, there is an inverse association between MMP-2 and MMP-9 in keratocystic odontogenic tumors (p=0.03; rs=-0.660), indicating activity in different regions.
authors.affiliation: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors.forename: Rafaela
authors.initials: R
authors.lastname: Scariot
authors.affiliation: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors.forename: Lucas Caetano
authors.initials: LC
authors.lastname: Uetanabaro
authors.affiliation: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors.forename: Melissa Rodrigues de
authors.initials: MR
authors.lastname: Araujo
authors.affiliation: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors.forename: Joao
authors.initials: J
authors.lastname: Zielak
authors.affiliation: Graduate Program in Dentistry, Positivo University, Curitiba, PR, Brazil.
authors.forename: Allan Fernando
authors.initials: AF
authors.lastname: Giovanini
authors.affiliation: Graduate Program in Dentistry, UFPR - Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Delson João da
authors.initials: DJ
authors.lastname: Costa
authors.affiliation: Graduate Program in Dentistry, UFPR - Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Nelson Luis Barbosa
authors.initials: NL
authors.lastname: Rebellato
authors.affiliation: Graduate Program in Dentistry, UFPR - Federal University of Paraná, Curitiba, PR, Brazil.
authors.forename: Renato Cordeiro
authors.initials: RC
authors.lastname: Gugisch
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D009808:Odontogenic Tumors / Q000000981:diagnostic imaging* / Q000201:enzymology*; D011862:Radiography, Panoramic
chemical_list: D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9