title: Enrichment of phosphorylated peptides and proteins by selective precipitation methods.
abstract: Protein phosphorylation is one of the most prominent post-translational modifications involved in the regulation of cellular processes. Fundamental understanding of biological processes requires appropriate bioanalytical methods for selectively enriching phosphorylated peptides and proteins. Most of the commonly applied enrichment approaches include chromatographic materials including Fe(3+)-immobilized metal-ion affinity chromatography or metal oxides. In the last years, the introduction of several non-chromatographic isolation technologies has increasingly attracted the interest of many scientists. Such approaches are based on the selective precipitation of phosphorylated peptides and proteins by applying various metal cations. The excellent performance of precipitation-based enrichment methods can be explained by the absence of any stationary phase, resin or sorbent, which usually leads to unspecific binding. This review provides an overview of recently published methods for the selective precipitation of phosphorylated peptides and proteins.
authors.affiliation: Institute of Analytical Chemistry & Radiochemistry, University of Innsbruck, Innrain 80/82, A-6020 Innsbruck, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Rainer
authors.forename: Günther K
authors.initials: GK
authors.lastname: Bonn
mesh_terms: D002412:Cations / Q000737:chemistry; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D028581:Lanthanoid Series Elements / Q000737:chemistry; D010748:Phosphopeptides / Q000032:analysis* / Q000302:isolation & purification; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002412:Cations; D028581:Lanthanoid Series Elements; D010748:Phosphopeptides; D011506:Proteins