title: In vivo cross-linking followed by polyA enrichment to identify yeast mRNA binding proteins.
abstract: mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, JSCBB Room B450, 3415 Colorado Ave, UCB 596, Boulder, CO, 80303, USA, sarah.f.mitchell@colorado.edu.
authors.forename: Sarah F
authors.initials: SF
authors.lastname: Mitchell
authors.forename: Roy
authors.initials: R
authors.lastname: Parker
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism; D011061:Poly A / Q000737:chemistry*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011061:Poly A