title: Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26.
abstract: In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells.
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. rjrenato@ig.com.br.
authors.forename: Renato Martins
authors.initials: RM
authors.lastname: da Silva
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. babifal@yahoo.com.br.
authors.forename: Bárbara Della
authors.initials: BD
authors.lastname: Noce
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. kmhilaf@hotmail.com.
authors.forename: Camila Fernanda
authors.initials: CF
authors.lastname: Waltero
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. eveniltonpessoa@yahoo.com.br.
authors.forename: Evenilton Pessoa
authors.initials: EP
authors.lastname: Costa
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. leoabreu@gmail.com.
authors.forename: Leonardo Araujo
authors.initials: LA
authors.lastname: de Abreu
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Kita-ku Sapporo 060-0818, Japan. naftalygitahi@yahoo.com.
authors.forename: Naftaly Wang'ombe
authors.initials: NW
authors.lastname: Githaka
authors.affiliation: Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, NUPEM-UFRJ/Macaé, Campus Macaé, Avenida São José do Barreto, São José do Barreto, CEP 27965-045 Macaé, RJ, Brazil. jlcmoraes@yahoo.com.br.
authors.forename: Jorge
authors.initials: J
authors.lastname: Moraes
authors.affiliation: Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, NUPEM-UFRJ/Macaé, Campus Macaé, Avenida São José do Barreto, São José do Barreto, CEP 27965-045 Macaé, RJ, Brazil. hgomes2@yahoo.com.br.
authors.forename: Helga Fernandes
authors.initials: HF
authors.lastname: Gomes
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Kita-ku Sapporo 060-0818, Japan. konnai@vetmed.hokudai.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: Center of Biotechnology, Federal University of Rio Grande do Sul, C.P. 15005, Av. Bento Gonçalves 9500, Prédio 43421, Campos do Vale, CEP 91501-970 Porto Alegre, RS, Brazil. itabajara.vaz@ufrgs.br.
authors.forename: Itabajara da Silva
authors.initials: Ida S
authors.lastname: Vaz
authors.affiliation: Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Kita-ku Sapporo 060-0818, Japan. kazu@chem.kyushu-univ.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ohashi
authors.affiliation: Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil. carloslogullo@yahoo.com.br.
authors.forename: Carlos
authors.initials: C
authors.lastname: Logullo
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D005943:Gluconeogenesis; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D048494:Rhipicephalus / Q000166:cytology / Q000196:embryology / Q000235:genetics / Q000378:metabolism*
chemical_list: D005947:Glucose