title: The effect of 5-amino-3-methyl-4-isoxazolecarboxylic acid hydrazide on lymphocyte subsets and humoral immune response in SRBC-immunized mice.
abstract: 5-Amino-3-methyl-4-isoxazolecarboxylic acid hydrazide is a non-cytotoxic synthetic isoxazole derivative with considerable immunomodulatory properties demonstrated in in vitro experiments. The aim of this study was to investigate the influence of this compound, depending on the dosage and schedule of treatment, on lymphocyte subsets in non-immunized mice and humoral immune response in SRBC (sheep red blood cells)-immunized mice. An analysis of lymphocyte subsets was carried out by flow cytometry, using specific monoclonal antibodies stained with fluorescein isothiocyanate (FITC) or phycoerythrin (PE). In the SRBC-immunized mice, the influence of the compound on the humoral response was determined, depending on the time of administration relative to the antigen. The number of plaque forming cells (PFC) was determined by a local hemolysis technique in an agar gel. Total and 2-mercaptoethanol resistant serum agglutination titers were defined by active hemagglutination test carried out on microplates. The investigated hydrazide was able to modulate the percentage and absolute number of T lymphocyte subsets in the thymus, and T and B lymphocytes in the peripheral lymphatic organs. It also enhanced humoral immune response in SRBC-immunized mice by increasing the number of cells producing hemolytic anti-SRBC antibodies (PFC) and by augmenting the level of total and 2-mercaptoethanol resistant hemagglutinin. The present study showed modulatory effects of 5-amino-3-methyl-4-isoxazolecarboxylic acid hydrazide on lymphocyte subsets and humoral immune response in mice. This compound could be potentially useful for the treatment of autoimmune diseases, infections or as an adjuvant for boosting the efficacy of vaccines.
authors.affiliation: Faculty of Veterinary Medicine, Department of Biochemistry, Pharmacology and Toxicology, Wrocław University of Environmental and Life Sciences , Wrocław , Poland and.
authors.forename: Angelika
authors.initials: A
authors.lastname: Drynda
authors.forename: Bożena
authors.initials: B
authors.lastname: Obmińska-Mrukowicz
authors.forename: Marcin
authors.initials: M
authors.lastname: Mączyński
authors.forename: Stanisław
authors.initials: S
authors.lastname: Ryng
mesh_terms: D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D056724:Immunity, Humoral / Q000187:drug effects / Q000276:immunology*; D007114:Immunization / Q000379:methods; D007555:Isoxazoles / Q000737:chemistry / Q000494:pharmacology*; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012756:Sheep
chemical_list: D002264:Carboxylic Acids; D007555:Isoxazoles
keywords: B and T lymphocyte subsets; flow cytometry; immunomodulation; isoxazole derivative; mice