title: Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia.
abstract: Phylogenetic group D extraintestinal pathogenic Escherichia coli (ExPEC), including O15:K52:H1 and clonal group A, have spread globally and become fluoroquinolone-resistant. Here we investigated the role of canine feces as a reservoir of these (and other) human-associated ExPEC and their potential as canine pathogens. We characterized and compared fluoroquinolone-resistant E. coli isolates originally identified as phylogenetic group D from either the feces of hospitalized dogs (n = 67; 14 dogs) or extraintestinal infections (n = 53; 33 dogs). Isolates underwent phylogenetic grouping, random amplified polymorphic DNA (RAPD) analysis, virulence genotyping, resistance genotyping, human-associated ExPEC O-typing, and multi-locus sequence typing. Five of seven human-associated sequence types (STs) exhibited ExPEC-associated O-types, and appeared in separate RAPD clusters. The largest subgroup (16 fecal, 26 clinical isolates) were ST354 (phylogroup F) isolates. ST420 (phylogroup B2); O1-ST38, O15:K52:H1-ST393, and O15:K1-ST130 (phylogroup D); and O7-ST457, and O1-ST648 (phylogroup F) were also identified. Three ST-specific RAPD sub-clusters (ST354, ST393, and ST457) contained closely related isolates from both fecal or clinical sources. Genes encoding CTX-M and AmpC β-lactamases were identified in isolates from five STs. Major human-associated fluoroquinolone-resistant ± extended-spectrum cephalosporin-resistant ExPEC of public health importance may be carried in dog feces and cause extraintestinal infections in some dogs.
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, NSW 2568, Australia.
authors.forename: SiYu
authors.initials: S
authors.lastname: Guo
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Wakeham
authors.affiliation: Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, NSW 2568, Australia.
authors.forename: Huub J M
authors.initials: HJ
authors.lastname: Brouwers
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia.
authors.forename: Rowland N
authors.initials: RN
authors.lastname: Cobbold
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, Australia.
authors.forename: Sam
authors.initials: S
authors.lastname: Abraham
authors.affiliation: School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; Biosecurity Sciences Laboratory, Biosecurity Queensland, Department of Agriculture, Fisheries and Forestry, Coopers Plains QLD 4108, Australia.
authors.forename: Joanne L
authors.initials: JL
authors.lastname: Mollinger
authors.affiliation: Infectious Diseases (111F), VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA.
authors.forename: James R
authors.initials: JR
authors.lastname: Johnson
authors.affiliation: Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, NSW 2568, Australia.
authors.forename: Toni A
authors.initials: TA
authors.lastname: Chapman
authors.affiliation: School of Biology, The Australian National University, Acton, ACT 0200, Australia.
authors.forename: David M
authors.initials: DM
authors.lastname: Gordon
authors.affiliation: University Veterinary Teaching Hospital, Sydney, Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia.
authors.forename: Vanessa R
authors.initials: VR
authors.lastname: Barrs
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, SA 5371, Australia. Electronic address: darren.trott@adelaide.edu.au.
authors.forename: Darren J
authors.initials: DJ
authors.lastname: Trott
mesh_terms: D000818:Animals; D001315:Australia; D004283:Dog Diseases / Q000276:immunology / Q000382:microbiology*; D004285:Dogs; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology* / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D024841:Fluoroquinolones / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D014774:Virulence / Q000276:immunology
chemical_list: D024841:Fluoroquinolones
keywords: Extended-spectrum cephalosporin-resistant; Extraintestinal pathogenic Escherichia coli; Fluoroquinolone-resistant; Phylogenetic group D