title: Antibody-mediated complement C3b/iC3b binding to group B Streptococcus in paired mother and baby serum samples in a refugee population on the Thailand-Myanmar border.
abstract: Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal sepsis and meningitis. In this study, we determined antibody-mediated deposition of complement C3b/iC3b onto the bacterial cell surface of GBS serotypes Ia, Ib, II, III, and V. This was determined for 520 mother and umbilical cord serum sample pairs obtained at the time of birth from a population on the Thailand-Myanmar border. Antibody-mediated deposition of complement C3b/iC3b was detected to at least one serotype in 91% of mothers, despite a known carriage rate in this population of only 12%. Antibody-mediated C3b/iC3b deposition corresponded to known carriage rates, with the highest levels of complement deposition observed onto the most prevalent serotype (serotype II) followed by serotypes Ia, III, V, and Ib. Finally, neonates born to mothers carrying serotype II GBS at the time of birth showed higher antibody-mediated C3b/iC3b deposition against serotype II GBS than neonates born to mothers with no serotype II carriage. Assessment of antibody-mediated C3b/iC3b deposition against GBS may provide insights into the seroepidemiology of anti-GBS antibodies in mothers and infants in different populations.
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: Jenny
authors.initials: J
authors.lastname: Herbert
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: Stephen
authors.initials: S
authors.lastname: Thomas
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Brookes
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia.
authors.forename: Claudia
authors.initials: C
authors.lastname: Turner
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand Cambodia-Oxford Medical Research Unit, Angkor Hospital for Children, Siem Reap, Cambodia.
authors.forename: Paul
authors.initials: P
authors.lastname: Turner
authors.affiliation: Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom Shoklo Malaria Research Unit, Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Mae Sot, Thailand.
authors.forename: Francois
authors.initials: F
authors.lastname: Nosten
authors.affiliation: Wellcome Centre for Global Health Research, Imperial College, London, United Kingdom Paediatric Infectious Diseases Research Group & Vaccine Institute, Institute of Infection and Immunity, St. George's, University of London, London, United Kingdom.
authors.forename: Kirsty
authors.initials: K
authors.lastname: Le Doare
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: Hudson
authors.affiliation: Paediatric Infectious Diseases Research Group & Vaccine Institute, Institute of Infection and Immunity, St. George's, University of London, London, United Kingdom.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Heath
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom.
authors.forename: Andrew
authors.initials: A
authors.lastname: Gorringe
authors.affiliation: Public Health England, Porton Down, Salisbury, United Kingdom stephen.taylor@phe.gov.uk.
authors.forename: Stephen
authors.initials: S
authors.lastname: Taylor
mesh_terms: D000907:Antibodies, Bacterial / Q000276:immunology*; D002353:Carrier State; D003179:Complement C3b / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D016555:Matched-Pair Analysis; D009035:Mothers; D002052:Myanmar / Q000453:epidemiology; D011485:Protein Binding; D012036:Refugees; D016036:Seroepidemiologic Studies; D012703:Serotyping; D013290:Streptococcal Infections / Q000276:immunology*; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism*; D013785:Thailand / Q000453:epidemiology
chemical_list: D000907:Antibodies, Bacterial; D003179:Complement C3b