title: Effect of liraglutide on proliferation and differentiation of human adipose stem cells.
abstract: Glucagon-Like Peptide-1 (GLP-1) receptor agonists, used as glucose-lowering drugs, also induce weight loss by inhibiting food intake. The present study was aimed at the assessment of the in vitro effects of the GLP-1 receptor agonist liraglutide on proliferation and differentiation of human adipose stem cells (ASC) obtained from subcutaneous adipose tissue of morbidly obese subjects undergoing bariatric surgery. Liraglutide (10-100 nM) significantly inhibited ASC proliferation and viability, with a maximum effect at 6 days of culture (45% and 50%, for liraglutide 10 and 100 nM, respectively); the effect was reverted by exendin 9-39. Glucose uptake was significantly reduced by liraglutide in a dose dependent manner. Treatment with liraglutide reduced intracellular lipid accumulation in differentiating ASC, together with FABP-4 mRNA expression (-18%, -23%, -46%, for 1 nM, 10 nM and 100 nM, respectively), whereas it stimulated adiponectin (APN) expression (1.86-, 2.64-, 2.28-fold increase, for 1 nM, 10 nM and 100 nM, respectively). Liraglutide exerts effects on human adipose cell precursors, inhibiting proliferation and differentiation, while stimulating the expression of the insulin-sensitizing adipokine APN. These effects could contribute to the actions of GLP-1 receptor agonists on body weight and insulin sensitivity.
authors.affiliation: Endocrinology Unit, Department Of Experimental and Clinical Biomedical Sciences, University of Florence, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Cantini
authors.affiliation: Endocrinology Unit, Department Of Experimental and Clinical Biomedical Sciences, University of Florence, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Di Franco
authors.affiliation: Endocrinology Unit, Department Of Experimental and Clinical Biomedical Sciences, University of Florence, Italy.
authors.forename: Jinous
authors.initials: J
authors.lastname: Samavat
authors.affiliation: Endocrinology Unit, Department Of Experimental and Clinical Biomedical Sciences, University of Florence, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Forti
authors.affiliation: Diabetes Agency, Azienda Ospedaliera-Universitaria Careggi, Florence, Italy. Electronic address: edoardo.mannucci@unifi.it.
authors.forename: Edoardo
authors.initials: E
authors.lastname: Mannucci
authors.affiliation: Endocrinology Unit, Department Of Experimental and Clinical Biomedical Sciences, University of Florence, Italy. Electronic address: michaela.luconi@unifi.it.
authors.forename: Michaela
authors.initials: M
authors.lastname: Luconi
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis; D000328:Adult; D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000368:Aged; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005260:Female; D052216:Glucagon-Like Peptide 1 / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D000069450:Liraglutide; D008297:Male; D008875:Middle Aged; D050151:Subcutaneous Fat / Q000166:cytology
chemical_list: D007004:Hypoglycemic Agents; D000069450:Liraglutide; D052216:Glucagon-Like Peptide 1
keywords: Adipogenesis; Adipose stem cells; Glucagone-Like-Peptide-1; Weight loss