title: [The effects of Kangai injection on enzyme activities of macrophages in rats].
abstract: OBJECTIVE
  To study the effects of Kangai injection on the enzyme activities of macrophages and morphology of spleen and thymus from rats.
  
  
  METHODS
  Twenty four male SD rats were randomly divided into two groups (n = 12), normal control group and experimental group. The rats in experimental group were injected with Kangai injection at the dosage of 5 ml/kg x d for 30 days peritoneally and those in control group were injected with nomal saline at the same volume. The content of supermicro protein was assayed by BCA method, the activities of lactate dehydrogenase (LDH), glutathione peroxidase(GSH-Px), and inducible nitric oxide synthase (iNOS) from alveolar macrophages(AM) and peritoneal macrophages (PM) were detected biochemically. The activities of acid phosphatase (ACP), superoxide dismutase(SOD) and succinate dehydrogenase (SDH) from AM and PM were detected by ELISA. The morphology of spleen and thymus were observed by light microscopy.
  
  
  RESULTS
  The activities of LDH, GSH-Px and iNOS within AM and PM from experimental group were increased significantly compared with those of control group (P < 0.05). The activities of ACP, SOD and SDH in AM and PM from experimental group were also higher than those from control group (P < 0.05). Microscopically, there was thickening of peripheral arterial lymphatic sheath, enlargement of splenic lymphoid nodules with expended germinal center in the spleen of experimental group. There was no significant difference in the mophology of thymus between the two groups.
  
  
  CONCLUSIONS
  Kangai injection may improve immune function by activating macrophages.
authors.affiliation: First Affiliated  Hospital, Wenzhou Medical University, China.
authors.forename: Han-Bin
authors.initials: HB
authors.lastname: Chen
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Ying
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Zhao
authors.forename: Yi-Xuan
authors.initials: YX
authors.lastname: Xiang
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D007770:L-Lactate Dehydrogenase; D005979:Glutathione Peroxidase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase; D013385:Succinate Dehydrogenase