title: Sequences spanning the leader-repeat junction  mediate CRISPR adaptation to phage in  Streptococcus thermophilus.
abstract: CRISPR-Cas systems are RNA-based immune systems that protect prokaryotes from invaders such as phages and plasmids. In adaptation, the initial phase of the immune response, short foreign DNA fragments are captured and integrated into host CRISPR loci to provide heritable defense against encountered foreign nucleic acids. Each CRISPR contains a ∼100-500 bp leader element that typically includes a transcription promoter, followed by an array of captured ∼35 bp sequences (spacers) sandwiched between copies of an identical ∼35 bp direct repeat sequence. New spacers are added immediately downstream of the leader. Here, we have analyzed adaptation to phage infection in Streptococcus thermophilus at the CRISPR1 locus to identify cis-acting elements essential for the process. We show that the leader and a single repeat of the CRISPR locus are sufficient for adaptation in this system. Moreover, we identified a leader sequence element capable of stimulating adaptation at a dormant repeat. We found that sequences within 10 bp of the site of integration, in both the leader and repeat of the CRISPR, are required for the process. Our results indicate that information at the CRISPR leader-repeat junction is critical for adaptation in this Type II-A system and likely other CRISPR-Cas systems.
authors.forename: Yunzhou
authors.initials: Y
authors.lastname: Wei
authors.forename: Megan T
authors.initials: MT
authors.lastname: Chesne
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Terns
authors.forename: Michael P
authors.initials: MP
authors.lastname: Terns
mesh_terms: D000222:Adaptation, Physiological; D001483:Base Sequence; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004279:DNA, Viral; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D017108:Streptococcus Phages / Q000235:genetics / Q000502:physiology*; D048249:Streptococcus thermophilus / Q000821:virology*
chemical_list: D004279:DNA, Viral