title: Screening of the miRNAs related to breast cancer and identification of its target genes.
abstract: OBJECTIVE
  To predict and verify the target genes of the miRNAs related to breast cancer beginning from the miRNA expression profile of human breast cancer.
  
  
  METHODS
  The total RNA was extracted from cancer tissues and the corresponding paracancerous tissues of eight breast cancer patients, and then miRNAs were separated. Human breast cancer cell line MDA-MB-231 and the normal mammary epithelial cell line HBL-100 were cultured, and the total RNA was extracted, respectively, with separation of miRNAs. The gene chip technology was used to analyze and detect the miRNAs differentially expressed in tissues and cancer cells. The miRNA expression profile of human breast cancer was obtained through chip scanning and data analysis.
  
  
  RESULTS
  Through dual-luciferase method, it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c, respectively.
  
  
  CONCLUSIONS
  miR-21 and miR-200c are related miRNAs to breast cancer, and they are associated with the occurrence and development of breast cancer.
authors.affiliation: Department of Breast, Henan Cancer Hospital affiliated Cancer Hospital of Zhengzhou University, China.
authors.forename: Xianfu
authors.initials: X
authors.lastname: Sun
authors.forename: Suxia
authors.initials: S
authors.lastname: Luo
authors.forename: Yaning
authors.initials: Y
authors.lastname: He
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Shao
authors.forename: Chaojun
authors.initials: C
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.forename: Shude
authors.initials: S
authors.lastname: Cui
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis*; D011230:Precancerous Conditions / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D016601:RNA-Binding Proteins / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; C533896:MIRN200 microRNA, human; C524940:MIRN21 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; C495390:PDCD10 protein, human; C114487:PDCD4 protein, human; D011518:Proto-Oncogene Proteins; D016601:RNA-Binding Proteins; D008156:Luciferases