title: A MALDI-Mass Spectrometry Imaging method applicable to different formalin-fixed paraffin-embedded human tissues.
abstract: Recent advancements in Matrix Assisted Laser Desorption/Ionisation (MALDI) Mass Spectrometry Imaging (MSI) technology have enabled the analysis of formalin-fixed paraffin-embedded (FFPE) tissue samples, unlocking a wealth of new proteomic information and facilitating the possibility of performing studies with higher statistical power as well as multi-centric collaborations within the field of proteomics research. However, current methods used to analyse these specimens are often time-consuming and they need to be modified when applied to human tissues of different origin. Here we present a reproducible and time-effective method that could address these aforementioned issues and widen the applicability of this technology to a number of challenging tissue types. Additionally, tissue molecular images show high spatial resolution and a strong correlation with the morphological features, enabling the identification of tissue morphology using statistically derived visualisation, without any prior knowledge.
authors.affiliation: Department of Health Sciences, Clinical Proteomics Unit, University Milan-Bicocca, Milan, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: De Sio
authors.forename: Andrew James
authors.initials: AJ
authors.lastname: Smith
authors.forename: Manuel
authors.initials: M
authors.lastname: Galli
authors.forename: Mattia
authors.initials: M
authors.lastname: Garancini
authors.forename: Clizia
authors.initials: C
authors.lastname: Chinello
authors.forename: Francesca
authors.initials: F
authors.lastname: Bono
authors.forename: Fabio
authors.initials: F
authors.lastname: Pagni
authors.forename: Fulvio
authors.initials: F
authors.lastname: Magni
mesh_terms: D001923:Brain Chemistry; D005557:Formaldehyde / Q000737:chemistry; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007668:Kidney / Q000737:chemistry; D057054:Molecular Imaging / Q000379:methods*; D016612:Paraffin Embedding / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D005557:Formaldehyde