title: Immobilization of DNA aptamers via plasma polymerized allylamine film to construct an endothelial progenitor cell-capture surface.
abstract: The endothelial progenitor cells (EPCs) capture stent has drawn increasing attentions and become one of the most promising concepts for the next generation vascular stent. In this regard, it is of great significance to immobilize a molecule with the ability to bind EPC for rapid in vivo endothelialization with high specificity. In this work, a facile two-step method aimed at constructing a coating with specific EPC capturing aptamers is reported. The processes involves as the first-step deposition of plasma polymerized allylamine (PPAam) on a substrate to introduce amine groups, followed by the electrostatic adsorption of a 34 bases single strand DNA sequence to the PPAam surface as a second step (PPAam-DNA). Grazing incidence attenuated total reflection Fourier transform infrared spectroscopy (GATR-FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the successful immobilization of the aptamers. Quartz crystal microbalance with dissipation (QCM-D) real time monitoring result shows that about 175 ng/cm(2) aptamers were conjugated onto the PPAam surface. The interactions between the modified surfaces and human umbilical vein endothelial cells (ECs), smooth muscle cells (SMCs), and murine induced EPCs derived from mesenchymal stem cells (MSCs) were also investigated. It was demonstrated that PPAam-DNA samples could capture more EPCs, and present a cellular friendly surface for the proliferation of both EPCs and ECs but no effect on the hyperplasia of SMCs. Also, the co-culture results of 3 types of cells confirmed that the aptamer could specifically bond EPCs rather than ECs and SMCs, suggesting the competitive adhesion advantage of EPCs to ECs and SMCs. These data demonstrate that the EPC aptamer has large potential for designing an EPC captured stent and other vascular grafts with targeted in situ endothelialization.
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Pengkai
authors.initials: P
authors.lastname: Qi
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; Laboratory of Biosensing and MicroMechatronics, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Yalong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: zhiluyang1029@126.com.
authors.forename: Zhilu
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; Laboratory of Biosensing and MicroMechatronics, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: tqfyezi7895@gmail.com.
authors.forename: Qiufen
authors.initials: Q
authors.lastname: Tu
authors.affiliation: Key Lab of Advanced Technology of Materials of Education Ministry, Southwest Jiaotong University, Chengdu 610031, China; School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China. Electronic address: nhuang@263.net.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000499:Allylamine / Q000097:blood / Q000737:chemistry*; D000818:Animals; D052157:Aptamers, Nucleotide / Q000097:blood / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D066026:Endothelial Progenitor Cells / Q000166:cytology*; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000097:blood / Q000737:chemistry*; D058105:Polymerization; D051381:Rats; D017207:Rats, Sprague-Dawley; D013499:Surface Properties; D017155:Wettability
chemical_list: D052157:Aptamers, Nucleotide; D061925:Immobilized Nucleic Acids; D000499:Allylamine
keywords: DNA aptamers; Endothelial progenitor cell; Endothelialization; Plasma surface modification; Vascular device