title: Association of interleukin 10 and transforming growth factor β gene polymorphisms with chronic idiopathic urticaria.
abstract: Transforming growth factor β (TGF-β) and interleukin 10 (IL-10) are two anti-inflammatory cytokines that are implicated in the pathogenesis of urticaria. The goal of this study was to examine the possible association of polymorphisms of TGF-β and IL-10 genes with susceptibility to chronic idiopathic urticaria (CIU). This study was conducted on 90 patients with CIU. Polymerase chain reaction (PCR) was done to determine the genotype at 5 polymorphic sites; TGF-β (codon10C/T and codon25G/C) and IL-10 (-1082G/A, -819C/T, and -592C/A). The C allele at codon 25 of TGF-β was more prevalent in CIU patients compared to controls (OR = 9.5, 95% CI = 5.4-16.8, P<0.001). Genotypes of CT and CG at 10 and 25 codons of TGF-β gene, respectively, and AG, CT, and CA for loci of -1082, -819, and -592 of IL-10 gene were significantly higher in CIU patients (P<0.001). In haplotype analysis, frequency of TGF-β haplotypes differed between patients with CIU and controls; CC haplotype was overrepresented, while CG and TG haplotypes were underrepresented (P<0.001). These results suggest that TGF-β and IL-10 genetic variability could contribute to susceptibility to CIU. Additionally, patients with CIU seem to have genotypes leading to high production of TGF-β and IL-10.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Tavakol
authors.forename: Masoud
authors.initials: M
authors.lastname: Movahedi
authors.affiliation: Ali Akbar Amirzargar, PhD, Molecular Immunology Research Center, Children's Medical Center Hospital, Dr Qarib St, Keshavarz Blvd, Tehran 14194, Iran; amirzara@tums.ac.ir.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Amirzargar
authors.forename: Zahra
authors.initials: Z
authors.lastname: Aryan
authors.forename: Alireza Zare
authors.initials: AZ
authors.lastname: Bidoki
authors.forename: Kimia
authors.initials: K
authors.lastname: Heidari
authors.forename: Samaneh
authors.initials: S
authors.lastname: Soltani
authors.forename: Mohammad
authors.initials: M
authors.lastname: Gharagozlou
authors.forename: Asghar
authors.initials: A
authors.lastname: Aghamohammadi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nabavi
authors.forename: Rasoul
authors.initials: R
authors.lastname: Nasiri
authors.forename: Alireza
authors.initials: A
authors.lastname: Ahmadvand
authors.forename: Nima
authors.initials: N
authors.lastname: Rezaei
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002908:Chronic Disease; D003062:Codon; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007492:Iran; D008297:Male; D011110:Polymorphism, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014581:Urticaria / Q000235:genetics*
chemical_list: D003062:Codon; D016212:Transforming Growth Factor beta; D016753:Interleukin-10