title: The squid ink polysaccharides protect tight junctions and adherens junctions from chemotherapeutic injury in the small intestinal epithelium of mice.
abstract: Gastrointestinal mucositis and infection by chemotherapy treatment are associated with alterations in the functioning of the intestinal barrier, due to the potential damage induced by anticancer drugs on the epithelial tight junctions and adheren junction. We aimed to study the protective effect of dietary polysaccharides on chemotherapy-induced injury in the epithelial cells. In the current study, using mice that were intraperitoneally injected with 50 mg/kg cyclophosphamide for 2 days, we reveal that polysaccharides from the ink of Ommastrephes bartrami (OBP) enhanced the mRNA and protein expression levels of Occludin, zonulae occluden (ZO)-1, and E-cadherin. Immunohistochemistry staining of ZO-1 and E-cadherin confirmed the increase in the mRNA and protein levels. OBP also remarkably enhanced the mRNA expression of other tight junction proteins, ZO-2, ZO-3, claudin-2, and cingulin. Our results may have important implications in host defense, especially the immunopotentiation function of OBP on the cyclophosphamide-induced epithelial cell injury, as well as intestinal disorders involving inflammation and infection.
authors.affiliation: a College of Food Science and Engineering , Ocean University of China , Qingdao , China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zuo
authors.forename: Lu
authors.initials: L
authors.lastname: Cao
authors.forename: Xuemin
authors.initials: X
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Changhu
authors.initials: C
authors.lastname: Xue
authors.forename: Qingjuan
authors.initials: Q
authors.lastname: Tang
mesh_terms: D022005:Adherens Junctions / Q000187:drug effects*; D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000009:adverse effects; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D057167:Claudins / Q000235:genetics; D003520:Cyclophosphamide / Q000009:adverse effects; D049832:Decapodiformes / Q000737:chemistry*; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000293:injuries; D007421:Intestine, Small; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects*; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D018906:Antineoplastic Agents, Alkylating; D015820:Cadherins; C489227:Cgn protein, mouse; D057167:Claudins; C113371:Cldn2 protein, mouse; D008565:Membrane Proteins; D062793:Occludin; D011134:Polysaccharides; D020011:Protective Agents; D012333:RNA, Messenger; D062826:Zonula Occludens-1 Protein; D003520:Cyclophosphamide