title: Cabut/dTIEG associates with the transcription factor Yorkie for growth control.
abstract: The Drosophila transcription factor Cabut/dTIEG (Cbt) is a growth regulator, whose expression is modulated by different stimuli. Here, we determine Cbt association with chromatin and identify Yorkie (Yki), the transcriptional co-activator of the Hippo (Hpo) pathway as its partner. Cbt and Yki co-localize on common gene promoters, and the expression of target genes varies according to changes in Cbt levels. Down-regulation of Cbt suppresses the overgrowth phenotypes caused by mutations in expanded (ex) and yki overexpression, whereas its up-regulation promotes cell proliferation. Our results imply that Cbt is a novel partner of Yki that is required as a transcriptional co-activator in growth control.
authors.affiliation: Departament de Genètica, Facultat de Biologia and Institut de Biomedicina (IBUB) de la Universitat de Barcelona, Barcelona, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Ruiz-Romero
authors.affiliation: Departament de Genètica, Facultat de Biologia and Institut de Biomedicina (IBUB) de la Universitat de Barcelona, Barcelona, Spain Centre for Genomic Regulation (CRG), Barcelona, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Blanco
authors.affiliation: Departamento de Genética, Facultad de Ciencias Biológicas, Universidad de Valencia, Valencia, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Paricio
authors.affiliation: Departament de Genètica, Facultat de Biologia and Institut de Biomedicina (IBUB) de la Universitat de Barcelona, Barcelona, Spain.
authors.forename: Florenci
authors.initials: F
authors.lastname: Serras
authors.affiliation: Departament de Genètica, Facultat de Biologia and Institut de Biomedicina (IBUB) de la Universitat de Barcelona, Barcelona, Spain mcorominas@ub.edu.
authors.forename: Montserrat
authors.initials: M
authors.lastname: Corominas
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation; D004330:Drosophila / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007605:Juvenile Hormones / Q000235:genetics*; D008954:Models, Biological; D009687:Nuclear Proteins / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000091102:YAP-Signaling Proteins; D000099317:Hippo Kinases
chemical_list: D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D007605:Juvenile Hormones; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D015534:Trans-Activators; D014157:Transcription Factors; D000091102:YAP-Signaling Proteins; C506602:Cbt protein, Drosophila; C503814:Yki protein, Drosophila; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases
keywords: Cabut; GAF; Yorkie; dTIEG; growth