title: [Leptin promotes the proliferation of airway smooth muscle cells and the expressions of HIF-1α and NF-κB of hypoxic rats].
abstract: OBJECTIVE
  To investigate the effect of leptin on the proliferation of airway smooth muscle cells (ASMCs) and the expressions of hypoxia-inducible factor-1α (HIF-1α) and nuclear factor-kappa B (NF-κB) of hypoxic rats.
  
  
  METHODS
  The rat ASMCs were cultured under normoxic and hypoxic states. The hypoxic cells were divided into hypoxia group, leptin 50 μg/L hypoxia group (L50 group), leptin 100 μg/L hypoxia group (L100 group), leptin 200 μg/L hypoxia group (L200 group), leptin 200 μg/L and leptin receptor antibody hypoxia group (ob-R antibody group) by random number table. All the groups are cultured for 24 hours. Then the CCK-8 method was used to assay cell proliferation rate, and Western blotting and real-time RT-PCR to measure the expressions of HIF-1α and NF-κB at protein and mRNA levels.
  
  
  RESULTS
  Compared with the normoxic group, each hypoxia group had significantly increased cell proliferation . Compared with the hypoxia group, cell proliferation rate was significantly raised in L50, L100 and L200 groups, and it was positively correlated with the concentration (r=0.992). Compared with L50, L100 and L200 groups, the ob-R antibody group showed significantly decreased cell proliferation rate. Compared with the normoxic group, each hypoxic group has increased expressions of HIF-1α and NF-κB mRNA and proteins; compared with the hypoxia group, the expressions were significantly elevated in the L50, L100 and L200 groups and showed a positive correlation with the concentration; but the expressions were reduced in the ob-R antibody group as compared with L50, L100 and L200 groups.
  
  
  CONCLUSIONS
  Leptin can promote rat ASMCs proliferation and the expressions of HIF-1α and NF-κB under hypoxic condition.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital, Xuzhou Medical College, Xuzhou 221000, China.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Shi
authors.affiliation: Department of Medicine, Central Hospital, Datun Coal-Electricity (Group) CO., LTD, Xuzhou 221611, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital, Xuzhou Medical College, Xuzhou 221000, China.
authors.forename: Jiechen
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital, Xuzhou Medical College, Xuzhou 221000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital, Xuzhou Medical College, Xuzhou 221000, China.
authors.forename: Shuyang
authors.initials: S
authors.lastname: Zhu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D006801:Humans; D000860:Hypoxia / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D020738:Leptin / Q000378:metabolism*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012137:Respiratory System / Q000166:cytology* / Q000378:metabolism / Q000503:physiopathology
chemical_list: D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020738:Leptin; D016328:NF-kappa B