title: Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence.
abstract: We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
authors.affiliation: Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; E-Mails: c.winterflood@pci.uzh.ch (C.M.W.); t.ruckstuhl@pci.uzh.ch (T.R.).
authors.forename: Christian M
authors.initials: CM
authors.lastname: Winterflood
authors.affiliation: Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; E-Mails: c.winterflood@pci.uzh.ch (C.M.W.); t.ruckstuhl@pci.uzh.ch (T.R.).
authors.forename: Thomas
authors.initials: T
authors.lastname: Ruckstuhl
authors.affiliation: Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; E-Mails: c.winterflood@pci.uzh.ch (C.M.W.); t.ruckstuhl@pci.uzh.ch (T.R.).
authors.forename: Stefan
authors.initials: S
authors.lastname: Seeger
keywords: interferon-gamma; one-step immunoassay; polymer test tube; supercritical angle fluorescence