title: A method for determining the actual rate of orientation switching of DNA self-assembled monolayers using optical and electrochemical frequency response analysis.
abstract: Electrostatic control of the orientation of fluorophore-labeled DNA strands immobilized on an electrode surface has been shown to be an effective bioanalytical tool. Modulation techniques and later time-resolved measurements were used to evaluate the kinetics of the switching between lying and standing DNA conformations. These measurements, however, are the result of a convolution between the DNA "switching" response time and the other frequency limited responses in the measurement. In this work, a method for analyzing the response of a potential driven DNA sensor is presented by calculating the potential effectively dropped across the electrode interface (using electrochemical impedance spectroscopy) as opposed to the potential applied to the electrochemical cell. This effectively deconvolutes the effect of the charging time on the observed frequency response. The corrected response shows that DNA is able to switch conformation faster than previously reported using modulation techniques. This approach will ensure accurate measurements independent of the electrochemical system, removing the uncertainty in the analysis of the switching response, enabling comparison between samples and measurement systems.
authors.affiliation: Department of Chemistry, University of British Columbia , 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Casanova-Moreno
authors.forename: D
authors.initials: D
authors.lastname: Bizzotto
mesh_terms: D004247:DNA / Q000032:analysis* / Q000138:chemical synthesis*; D055664:Electrochemical Techniques; D009690:Nucleic Acid Conformation; D055096:Optical Devices
chemical_list: D004247:DNA