title: Molecular and biochemical characterization of CTX-M-131, a natural Asp240Gly variant derived from CTX-M-2, produced by a Providencia rettgeri clinical strain in São Paulo, Brazil.
abstract: CTX-M-131 is a natural Asp240Gly variant from the CTX-M-2 group detected in a Providencia rettgeri clinical strain from Brazil. Molecular analysis showed that blaCTX-M-131 was inserted in a complex class 1 integron harbored by a 112-kb plasmid, which has not been previously described as a platform for CTX-M-encoding genes with the Asp240Gly mutation. Steady-state kinetic parameters showed that the enzyme has a typical cefotaximase catalytic profile and an enhanced activity against ceftazidime.
authors.affiliation: School of Public Health, University of São Paulo, São Paulo, Brazil milenadropa@gmail.com.
authors.forename: Milena
authors.initials: M
authors.lastname: Dropa
authors.affiliation: School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Barbara
authors.initials: B
authors.lastname: Ghiglione
authors.affiliation: School of Public Health, University of São Paulo, São Paulo, Brazil.
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Matté
authors.affiliation: School of Public Health, University of São Paulo, São Paulo, Brazil.
authors.forename: Livia Carminato
authors.initials: LC
authors.lastname: Balsalobre
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Nilton
authors.initials: N
authors.lastname: Lincopan
authors.affiliation: School of Public Health, University of São Paulo, São Paulo, Brazil.
authors.forename: Glavur Rogério
authors.initials: GR
authors.lastname: Matté
authors.affiliation: School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Gutkind
authors.affiliation: School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina.
authors.forename: Pablo
authors.initials: P
authors.lastname: Power
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil; D002442:Ceftazidime / Q000494:pharmacology; D016023:Integrins / Q000235:genetics; D007700:Kinetics; D010957:Plasmids / Q000235:genetics; D011532:Providencia / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D016023:Integrins; D002442:Ceftazidime; C071167:beta-lactamase CTX-2; D001618:beta-Lactamases