title: Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes.
abstract: The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.
authors.forename: Jia-Le
authors.initials: JL
authors.lastname: Song
authors.affiliation: Department of Pharmacy, Northern Jiangsu People's Hospital Affiliated to Yangzhou University (Clinical Medical College of Yangzhou University), Yangzhou, Jiangsu 225001,People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
keywords: Antioxidant enzymes; HaCaT keratinocytes; Lindera coreana; Reactive oxygen species (ROS); Ultraviolet (UV)