title: Identification of a human papillomavirus-associated oncogenic miRNA panel in human oropharyngeal squamous cell carcinoma validated by bioinformatics analysis of the Cancer Genome Atlas.
abstract: High-risk human papillomavirus (HPV) is a causative agent for an increasing subset of oropharyngeal squamous cell carcinomas (OPSCCs), and current evidence supports these tumors as having identifiable risk factors and improved response to therapy. However, the biochemical and molecular alterations underlying the pathobiology of HPV-associated OPSCC (designated HPV(+) OPSCC) remain unclear. Herein, we profile miRNA expression patterns in HPV(+) OPSCC to provide a more detailed understanding of pathologic molecular events and to identify biomarkers that may have applicability for early diagnosis, improved staging, and prognostic stratification. Differentially expressed miRNAs were identified in RNA isolated from an initial clinical cohort of HPV(+/-) OPSCC tumors by quantitative PCR-based miRNA profiling. This oncogenic miRNA panel was validated using miRNA sequencing and clinical data from The Cancer Genome Atlas and miRNA in situ hybridization. The HPV-associated oncogenic miRNA panel has potential utility in diagnosis and disease stratification and in mechanistic elucidation of molecular factors that contribute to OPSCC development, progression, and differential response to therapy.
authors.affiliation: Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri.
authors.forename: Daniel L
authors.initials: DL
authors.lastname: Miller
authors.affiliation: Department of Health Management and Informatics, University of Missouri School of Medicine, Columbia, Missouri; Department of Statistics, University of Missouri School of Medicine, Columbia, Missouri.
authors.forename: J Wade
authors.initials: JW
authors.lastname: Davis
authors.affiliation: Department of Pathology and Anatomical Sciences, University of Missouri School of Medicine, Columbia, Missouri.
authors.forename: Kristen H
authors.initials: KH
authors.lastname: Taylor
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, Indiana; Harper Cancer Research Institute, University of Notre Dame, South Bend, Indiana.
authors.forename: Jeff
authors.initials: J
authors.lastname: Johnson
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, Indiana; Harper Cancer Research Institute, University of Notre Dame, South Bend, Indiana.
authors.forename: Zonggao
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Biochemistry, Indiana University South Bend, South Bend, Indiana.
authors.forename: Russell
authors.initials: R
authors.lastname: Williams
authors.affiliation: Department of Surgery, University of Missouri School of Medicine, Columbia, Missouri.
authors.forename: Ulus
authors.initials: U
authors.lastname: Atasoy
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri; Department of Otolaryngology Head and Neck Surgery, Washington University School of Medicine, St. Louis, Missouri.
authors.forename: James S
authors.initials: JS
authors.lastname: Lewis
authors.affiliation: Department of Chemistry and Biochemistry, University of Notre Dame, South Bend, Indiana; Harper Cancer Research Institute, University of Notre Dame, South Bend, Indiana. Electronic address: sstack@nd.edu.
authors.forename: M Sharon
authors.initials: MS
authors.lastname: Stack
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000821:virology; D045744:Cell Line, Tumor; D019295:Computational Biology; D004279:DNA, Viral; D052162:Human papillomavirus 16; D006801:Humans; D035683:MicroRNAs; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000821:virology; D030361:Papillomavirus Infections / Q000235:genetics* / Q000473:pathology / Q000821:virology
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs