title: Shear stress is normalized in glomerular capillaries following ⅚ nephrectomy.
abstract: Loss of significant functional renal mass results in compensatory structural and hemodynamic adaptations in the nephron. While these changes have been characterized in several injury models, how they affect hemodynamic forces at the glomerular capillary wall has not been adequately characterized, despite their potential physiological significance. Therefore, we used intravital multiphoton microscopy to measure the velocity of red blood cells in individual glomerular capillaries of normal rats and rats subjected to ⅚ nephrectomy. Glomerular capillary blood flow rate and wall shear stress were then estimated using previously established experimental and mathematical models to account for changes in hematocrit and blood rheology in small vessels. We found little change in the hemodynamic parameters in glomerular capillaries immediately following injury. At 2 wk postnephrectomy, significant changes in individual capillary blood flow velocity and volume flow rate were present. Despite these changes, estimated capillary wall shear stress was unchanged. This was a result of an increase in capillary diameter and changes in capillary blood rheology in nephrectomized rats.
authors.affiliation: Division of Nephrology, Vanderbilt University Medical Center, Nashville, Tennessee; nick.ferrell@vanderbilt.edu.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Ferrell
authors.affiliation: Division of Nephrology, Indiana University School of Medicine, Indianapolis, Indiana;
authors.forename: Ruben M
authors.initials: RM
authors.lastname: Sandoval
authors.affiliation: Department of Biostatistics, Vanderbilt University, Nashville, Tennessee; and.
authors.forename: Aihua
authors.initials: A
authors.lastname: Bian
authors.affiliation: Division of Nephrology, Indiana University School of Medicine, Indianapolis, Indiana;
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Campos-Bilderback
authors.affiliation: Division of Nephrology, Indiana University School of Medicine, Indianapolis, Indiana; Roudebush Veterans Affairs Medical Center, Indianapolis, Indiana.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Molitoris
authors.affiliation: Division of Nephrology, Vanderbilt University Medical Center, Nashville, Tennessee;
authors.forename: William H
authors.initials: WH
authors.lastname: Fissell
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002196:Capillaries / Q000502:physiology*; D006400:Hematocrit; D018056:Hemorheology; D007678:Kidney Glomerulus / Q000502:physiology*; D008297:Male; D009392:Nephrectomy; D017208:Rats, Wistar; D012079:Renal Circulation; D051437:Renal Insufficiency / Q000503:physiopathology*; D013314:Stress, Mechanical
keywords: glomerular capillary; hemodynamics; shear stress; ⅚ nephrectomy