title: Screening antimutagenic and antiproliferative properties of extracts isolated from Jackfruit pulp (Artocarpus heterophyllus Lam).
abstract: The present focused on the study of the antimutagenic and antiproliferative potential of pulp Jackfruit (Artocarpus heterophyllus Lam) extract, using Salmonella typhimurium tester strains TA98 and TA100 with metabolic activation (S9) and a cancer cell line M12.C3.F6 (murine B-cell lymphoma), respectively. Jackfruit pulp extract was sequentially fractionated by chromatography (RP-HPLC) and each fraction was tested for antimutagenic and antiproliferative activities. The organic extracts obtained from Jackfruit pulp reduced the number of revertants caused by aflatoxin B1 (AFB1) and proliferation of cells M12.C3.F6; a dose-response relationship was showed. Sequential RP-HPLC fractionation of the active extracts produced both antimutagenic and/or antiproliferative fractions. These results suggested that the Jackfruit contained compounds with chemoprotective properties to reduce the mutagenicity of AFB1, also proliferation of a cancer cell line.
authors.affiliation: Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av. Tecnológico 2595, C.P. 63175 Tepic, Nayarit, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: Ruiz-Montañez
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Burgos-Hernández
authors.affiliation: Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av. Tecnológico 2595, C.P. 63175 Tepic, Nayarit, Mexico. Electronic address: montserratcalder@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Calderón-Santoyo
authors.affiliation: Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Mexico.
authors.forename: C M
authors.initials: CM
authors.lastname: López-Saiz
authors.affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de Sonora, C.P. 1658 Hermosillo, Sonora, Mexico.
authors.forename: C A
authors.initials: CA
authors.lastname: Velázquez-Contreras
authors.affiliation: Universidad Nacional Autónoma de México, Facultad de Química, Circuito Interior, Ciudad Universitaria, Col. Copilco, C.P. 04510 Coyoacán, D.F., Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Navarro-Ocaña
authors.affiliation: Laboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de Tepic, Av. Tecnológico 2595, C.P. 63175 Tepic, Nayarit, Mexico. Electronic address: arturoragazzo@hotmail.com.
authors.forename: J A
authors.initials: JA
authors.lastname: Ragazzo-Sánchez
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D031622:Artocarpus / Q000737:chemistry*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical; D004354:Drug Screening Assays, Antitumor; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016587:Antimutagenic Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D010936:Plant Extracts
keywords: Antimutagenic; Antioxidant; Antiproliferative; Chemoprotective properties; Jackfruit pulp; RP-HPLC