title: Decrease of colonization in the chicks' cecum and internal organs of Salmonella enterica serovar Pullorum by deletion of cpdB by Red system.
abstract: Salmonella enterica serovar Pullorum (S. Pullorum) is a worldwide poultry pathogen of considerable economic importance, particularly in those countries with a developing poultry industry. A variety of genes that affect S. Pullorum colonization in chickens had been identified. 2',3'-cyclic phosphodiesterase (cpdB) is the bifunctional enzyme which possess 2',3'-cyclic phosphodiesterase as well as 3'-nucleotidase activity. To assess the role of cpdB of S. Pullorum in colonization of cecum and internal organs in poultry, seven-day-old chicks were infected with 10(9) CFU/ml of a cpdB mutant and wild type strain. High number of cpdB mutant and wild type strain colonized the internal organs shortly after infection, but no colonization of cpdB mutant were observed from internal organs at day 10 post-infection, meanwhile, wild type bacteria in internal organs were observed at day 16 post-infection. Furthermore, the colonization of cpdB mutant in the cecum was seriously decreased from 6 days post-infection simultaneously wild type strain was increased and seriously decreased at day 8 post-infection. At day 12 post-infection, no cpdB mutant was observed from cecum, however high numbers of wild type strain were isolated at day 16 post-infection. It is concluded that cpdB is involved in long-term colonization of S. Pullorum in the chicks' cecum and internal organs. In addition, deletion of cpdB from S. Pullorum was not affect the morphology and growth of bacteria.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China; College of Life Sciences, Nankai University, Tianjin 300071, China; Northeast Agricultural University, Harbin 150030, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Huanan
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China. Electronic address: siguo_liu@hvri.ac.cn.
authors.forename: Siguo
authors.initials: S
authors.lastname: Liu
authors.affiliation: Northeast Agricultural University, Harbin 150030, China. Electronic address: ligx@neau.edu.cn.
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
mesh_terms: D000825:Animal Structures / Q000382:microbiology*; D000818:Animals; D002432:Cecum / Q000382:microbiology*; D002645:Chickens; D017353:Gene Deletion; D055786:Gene Knockout Techniques; D009708:Nucleotidases / Q000235:genetics*; D019779:Salmonella enterica / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D013997:Time Factors; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D037521:Virulence Factors; D009708:Nucleotidases; C040470:3'-nucleotidase
keywords: Colonization; Deletion; Invasion; Salmonella serovar Pullorum; cpdB gene