title: The effect of bone marrow concentrate and hyperbaric oxygen therapy on bone repair.
abstract: Neoangiogenesis represents an essential part of bone regeneration. Therefore the improvement of neovascularization is the subject of various research approaches. In addition autologous mesenchymal stem cells concentrate in combination with bone substitute materials have been shown to support bone regeneration. In a rabbit model we examined the proposed synergistic effect of hyperbaric oxygen therapy (HBOT) and bone marrow concentrate (BMC) with porous calcium phosphate granules (CPG) on neoangiogenesis and osseous consolidation of a critical- size defect. The animal groups treated with HBOT showed a significantly higher microvessel density (MVD) by immunhistochemistry. Furthermore HBOT groups presented a significantly larger amount of new bone formation histomorphometrically as well as radiologically. We conclude that the increase in perfusion as a result of increased angiogenesis may play a key role in the effects of HBOT and consequently promotes bone healing.
authors.affiliation: Department of Trauma and Handsurgery, Heinrich Heine University Hospital Duesseldorf, Moorenstr. 5, 40225, Duesseldorf, Germany.
authors.forename: J P
authors.initials: JP
authors.lastname: Grassmann
authors.forename: J
authors.initials: J
authors.lastname: Schneppendahl
authors.forename: M
authors.initials: M
authors.lastname: Sager
authors.forename: A R
authors.initials: AR
authors.lastname: Hakimi
authors.forename: M
authors.initials: M
authors.lastname: Herten
authors.forename: T T
authors.initials: TT
authors.lastname: Loegters
authors.forename: M
authors.initials: M
authors.lastname: Wild
authors.forename: M
authors.initials: M
authors.lastname: Hakimi
authors.forename: J
authors.initials: J
authors.lastname: Windolf
authors.forename: P
authors.initials: P
authors.lastname: Jungbluth
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000737:chemistry*; D001861:Bone Regeneration; D002454:Cell Differentiation; D049109:Cell Proliferation; D006931:Hyperbaric Oxygenation; D011817:Rabbits