title: Salicylic Acid Inhibits the Replication of Tomato bushy stunt virus by Directly Targeting a Host Component in the Replication Complex.
abstract: Although the plant hormone salicylic acid (SA) plays a central role in signaling resistance to viral infection, the underlying mechanisms are only partially understood. Identification and characterization of SA's direct targets have been shown to be an effective strategy for dissecting the complex SA-mediated defense signaling network. In search of additional SA targets, we previously developed two sensitive approaches that utilize SA analogs in conjunction with either a photoaffinity labeling technique or surface plasmon resonance-based technology to identify and evaluate candidate SA-binding proteins (SABPs) from Arabidopsis. Using these approaches, we have now identified several members of the Arabidopsis glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein family, including two chloroplast-localized and two cytosolic isoforms, as SABPs. Cytosolic GAPDH is a well-known glycolytic enzyme; it also is an important host factor involved in the replication of Tomato bushy stunt virus (TBSV), a single-stranded RNA virus. Using a yeast cell-free extract, an in vivo yeast replication system, and plant protoplasts, we demonstrate that SA inhibits TBSV replication. SA does so by inhibiting the binding of cytosolic GAPDH to the negative (-)RNA strand of TBSV. Thus, this study reveals a novel molecular mechanism through which SA regulates virus replication.
authors.affiliation: 1 Boyce Thompson Institute for Plant Research, Ithaca, NY 14853, U.S.A.;
authors.forename: Miaoying
authors.initials: M
authors.lastname: Tian
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Sasvari
authors.forename: Paulina Alatriste
authors.initials: PA
authors.lastname: Gonzalez
authors.forename: Giulia
authors.initials: G
authors.lastname: Friso
authors.forename: Elden
authors.initials: E
authors.lastname: Rowland
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Liu
authors.forename: Klaas J
authors.initials: KJ
authors.lastname: van Wijk
authors.forename: Peter D
authors.initials: PD
authors.lastname: Nagy
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Klessig
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000821:virology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000187:drug effects*; D020156:Salicylic Acid / Q000494:pharmacology*; D017864:Tombusvirus / Q000235:genetics*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D029681:Arabidopsis Proteins; C585192:GAPC1 protein, Arabidopsis; D024581:Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); D020156:Salicylic Acid