title: Membrane protein reconstitution for functional and structural studies.
abstract: Membrane proteins are involved in various critical biological processes, and studying membrane proteins represents a major challenge in protein biochemistry. As shown by both structural and functional studies, the membrane environment plays an essential role for membrane proteins. In vitro studies are reliant on the successful reconstitution of membrane proteins. This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes. Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed. Finally the applications of reconstitution techniques to study membrane proteins involved in Ca(2+) signaling are summarized.
authors.affiliation: Department of Biological Structure, University of Washington, Seattle, Washington, 98195, USA, LW32@uw.edu.
authors.forename: LiGuo
authors.initials: L
authors.lastname: Wang
authors.forename: LiGe
authors.initials: L
authors.lastname: Tonggu
mesh_terms: D003902:Detergents / Q000737:chemistry; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D013329:Structure-Activity Relationship
chemical_list: D003902:Detergents; D008565:Membrane Proteins