title: A projection selection method to improve image quality in optical projection tomography.
abstract: Optical projection tomography (OPT) is a very important imaging tool for a mesoscopic-scale. It can provide three dimensional (3D) transmission and emission imaging. However, high-resolution OPT is limited in depth of field (DOF) due to a high numerical aperture, which causes a poor performance of OPT in imaging large samples. Moreover, it is difficult to tune the focus plane (FP) to a fixed position where OPT always has the best image quality in different directions. To address these problems, we developed a projection selection method to improve DOF in OPT. In each direction, our method automatically selects the best projection from several projections with different FP. Then, we use a series of selected projections for 3D reconstruction. The experimental results demonstrate that our method can improve the image quality comparing to a fixed FP. Moreover, our method is flexible to be used in other OPT setups by adding a linear stage.
authors.forename: Jin
authors.initials: J
authors.lastname: Guo
authors.forename: Yujie
authors.initials: Y
authors.lastname: Yang
authors.forename: Di
authors.initials: D
authors.lastname: Dong
authors.forename: Liangliang
authors.initials: L
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Hui
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.lastname: Jie Tian
authors.lastname: Xia Liu
mesh_terms: D000465:Algorithms; D000818:Animals; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000254:growth & development; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional; D011679:Pupa / Q000033:anatomy & histology; D012469:Salivary Glands / Q000033:anatomy & histology; D041622:Tomography, Optical / Q000379:methods*