title: Comparative analysis of ADS gene promoter in seven Artemisia species.
abstract: Artemisinin is the most effective antimalarial drug that is derived from Artemisia annua. Amorpha-4,11-diene synthase (ADS) controls the first committed step in artemisinin biosynthesis. The ADS gene expression is regulated by transcription factors which bind to the cis-acting elements on the ADS promoter and are probably responsible for the ADS gene expression difference in the Artemisia species. To identify the elements that are significantly involved in ADS gene expression, the ADS gene promoter of the seven Artemisia species was isolated and comparative analysis was performed on the ADS promoter sequences of these species. Results revealed that some of the cis-elements were unique or in terms of number were more in the high artemisinin producer species, A. annua, than the other species. We have reported that the light-responsive elements, W-box, CAAT-box, 5'-UTR py-rich stretch, TATA-box sequence and tandem repeat sequences have been identified as important factors in the increased expression of ADS gene.
authors.affiliation: Agronomy and Plant Breeding Department, College of Agriculture, University of Tehran, Karaj, 31587-11167, Iran. mnaghavi@ut.ac.ir.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Ranjbar
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Naghavi
authors.forename: Hoshang
authors.initials: H
authors.lastname: Alizadeh
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence / Q000235:genetics; D031172:Artemisia annua / Q000235:genetics*; D037621:Artemisinins / Q000378:metabolism / Q000627:therapeutic use; D018506:Gene Expression Regulation, Plant; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
chemical_list: D037621:Artemisinins; D014157:Transcription Factors; C031327:artemisinin; D019883:Alkyl and Aryl Transferases; C403714:amorpha-4,11-diene synthase