title: Accelerated detection of viral particles by combining AC electric field effects and micro-Raman spectroscopy.
abstract: A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres) captured by an AC electric field between planar microelectrodes is presented with a methodology for analyzing the resulting spectra by comparing relative peak intensities. The same principle is applied to dielectrophoretically captured M13 phage particles whose presence is indirectly confirmed with micro-Raman spectroscopy using NeutrAvidin-Cy3 as a labeling molecule. It is concluded that the combination of electrokinetically driven virus sampling and micro-Raman based signal transduction provides a promising approach for time-efficient and in situ detection of viruses.
authors.affiliation: Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada. matthewrtomkins@gmail.com.
authors.forename: Matthew Robert
authors.initials: MR
authors.lastname: Tomkins
authors.affiliation: Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada. david.liao@chee.queensu.ca.
authors.forename: David Shiqi
authors.initials: DS
authors.lastname: Liao
authors.affiliation: Department of Chemical Engineering Queen's University, Kingston, ON K7L 3N6, Canada. docoslis@queensu.ca.
authors.forename: Aristides
authors.initials: A
authors.lastname: Docoslis
mesh_terms: D017104:Bacteriophage M13 / Q000302:isolation & purification*; D004560:Electricity; D008839:Microelectrodes; D011137:Polystyrenes / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014771:Virion / Q000302:isolation & purification*
chemical_list: D011137:Polystyrenes; D012825:Silicon