title: FAM83B is a novel biomarker for diagnosis and prognosis of lung squamous cell carcinoma.
abstract: Personalized therapy for non‑small cell lung cancer (NSCLC), particularly lung adenocarcinoma, has recently been significantly improved by the discovery of various molecular targets. However, this has not been the case for lung squamous cell carcinoma (SCC). In the present study, we identified the family with sequence similarity 83, member B (FAM83B) as a candidate marker for SCC through a comprehensive gene expression analysis and examined its correlations with various clinicopathological factors. The subjects of this study consisted of 215 patients with NSCLC who underwent complete resection from 2005 to 2011 at the Fukushima Medical University Hospital (Fukushima, Japan). They included 102 patients with adenocarcinoma and 113 with SCC. FAM83B expression was first examined in some of the samples by gene expression analysis and western blotting, and then all clinical specimens were evaluated by immunohistochemistry (IHC). The relationship between the quantitative values for IHC and clinicopathological factors was statistically analyzed. The results showed that FAM83B mRNA expression was significantly higher in SCC than in normal lung or adenocarcinoma (P<0.0001). Immunoblot analysis also confirmed this trend. Specimens containing >10% positive area for FAM83B were judged as 'positive'; 94.3% (107/113) of SCC and 14.7% (15/102) of adenocarcinoma were positive. Patients were divided into two subgroups according to expression (54 high‑expression and 53 low‑expression patients); the high‑expression group was associated with a better disease‑free survival (DFS) rate (P=0.042, log‑rank test). In conclusion, FAM83B may be a reliable diagnostic and prognostic biomarker for SCC. Detailed analyses of FAM83B function in lung cancer are required to understand how its expression is associated with better prognosis in SCC.
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Okabe
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Junji
authors.initials: J
authors.lastname: Ezaki
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Yamaura
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Muto
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Osugi
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Hirosumi
authors.initials: H
authors.lastname: Tamura
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Imai
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.affiliation: Nippon Gene Co., Ltd., Chiyoda, Tokyo 101‑0054, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Yanagisawa
authors.affiliation: Nippon Gene Co., Ltd., Chiyoda, Tokyo 101‑0054, Japan.
authors.forename: Reiko
authors.initials: R
authors.lastname: Honma
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Mitsukazu
authors.initials: M
authors.lastname: Gotoh
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Medical‑Industrial Translational Research Center, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Waguri
authors.affiliation: Department of Regenerative Surgery, Fukushima Medical University, School of Medicine, Fukushima 960‑1295, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics; D000077192:Adenocarcinoma of Lung; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D015331:Cohort Studies; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007564:Japan; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D012016:Reference Values
chemical_list: D014408:Biomarkers, Tumor; C000601379:FAM83B protein, human; D009363:Neoplasm Proteins