title: Redirecting metabolic flux in Saccharomyces cerevisiae through regulation of cofactors in UMP production.
abstract: Although it is generally known that cofactors play a major role in the production of different fermentation products, their role has not been thoroughly and systematically studied. To understand the impact of cofactors on physiological functions, a systematic approach was applied, which involved redox state analysis, energy charge analysis, and metabolite analysis. Using uridine 5'-monophosphate metabolism in Saccharomyces cerevisiae as a model, we demonstrated that regulation of intracellular the ratio of NADPH to NADP(+) not only redistributed the carbon flux between the glycolytic and pentose phosphate pathways, but also regulated the redox state of NAD(H), resulting in a significant change of ATP, and a significantly altered spectrum of metabolic products.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Xin mofan Road 5, Nanjing, 210009, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Qingguo
authors.initials: Q
authors.lastname: Liu
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Chen
authors.forename: Jinglan
authors.initials: J
authors.lastname: Wu
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Chenjie
authors.initials: C
authors.lastname: Zhu
authors.forename: Hanjie
authors.initials: H
authors.lastname: Ying
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D005285:Fermentation; D006019:Glycolysis; D053858:Metabolic Networks and Pathways; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D010427:Pentose Phosphate Pathway; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014542:Uridine Monophosphate / Q000096:biosynthesis*
chemical_list: D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D014542:Uridine Monophosphate