title: Development of a walleye cell line and use to study the effects of temperature on infection by viral haemorrhagic septicaemia virus group IVb.
abstract: A cell line, WE-cfin11f, with a fibroblast-like morphology was developed from a walleye caudal fin and used to study the intersection of thermobiology of walleye, Sander vitreus (Mitchill), with the thermal requirements for replication of viral haemorrhagic septicaemia virus (VHSV) IVb. WE-cfin11f proliferated from 10 to 32 °C and endured as a monolayer for at least a week at 1-34 °C. WE-cfin11f adopted an epithelial shape and did not proliferate at 4 °C. Adding VHSV IVb to cultures at 4 and 14 °C but not 26 °C led to cytopathic effects (CPE) and virus production. At 4 °C, virus production developed more slowly, but Western blotting showed more N protein accumulation. Infecting monolayer cultures at 4 °C for 7 days and then shifting them to 26 °C resulted in the monolayers being broken in small areas by CPE, but with time at 26 °C, the monolayers were restored. These results suggest that at 26 °C, the VHSV IVb life cycle stages responsible for CPE can be completed, but the production of virus and the initiation of infections cannot be accomplished.
authors.affiliation: Department of Biology, University of Waterloo, Waterloo, ON, Canada.
authors.forename: N T K
authors.initials: NT
authors.lastname: Vo
authors.forename: A W
authors.initials: AW
authors.lastname: Bender
authors.forename: L E J
authors.initials: LE
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Lumsden
authors.forename: N
authors.initials: N
authors.lastname: Lorenzen
authors.forename: B
authors.initials: B
authors.lastname: Dixon
authors.forename: N C
authors.initials: NC
authors.lastname: Bols
mesh_terms: D000818:Animals; D002460:Cell Line; D031941:Hemorrhagic Septicemia, Viral / Q000503:physiopathology* / Q000821:virology; D029141:Novirhabdovirus / Q000502:physiology*; D010471:Perches; D013696:Temperature; D014779:Virus Replication
keywords: VHSV; caudal fin; fibroblasts; thermobiology; thermovirology; walleye