title: Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers.
abstract: OBJECTIVE
  We have investigated the effect of surface charge of model lipid membranes on their interactions with dendriplexes formed by HIV-derived peptides and 2 types of positively charged carbosilane dendrimers (CBD).
  
  
  METHODS
  Interaction of dendriplexes with lipid membranes was measured by fluorescence anisotropy, dynamic light scattering and Langmuir-Blodgett techniques. The morphology of the complexes was examined by transmission electron microscopy.
  
  
  RESULTS
  All dendriplexes independent of the type of peptide interacted with model lipid membranes. Negatively charged vesicles composed of a mixture of DMPC/DPPG interacted more strongly, and it was accompanied by an increase in anisotropy of the fluorescent probe localized in polar domain of lipid bilayers. There was also an increase in surface pressure of the lipid monolayers. Mixing negatively charged liposomes with dendriplexes increased liposome size and made their surface charges more positive.
  
  
  CONCLUSIONS
  HIV-peptide/dendrimer complexes interact with model lipid membranes depending on their surface charge. Carbosilane dendrimers can be useful as non-viral carriers for delivering HIV-peptides into cells.
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: maksion@biol.uni.lodz.pl.
authors.forename: Maksim
authors.initials: M
authors.lastname: Ionov
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Ciepluch
authors.affiliation: Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Garaiova
authors.affiliation: Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia.
authors.forename: Sophie
authors.initials: S
authors.lastname: Melikishvili
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódż, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Michlewska
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódż, Poland.
authors.forename: Łucja
authors.initials: Ł
authors.lastname: Balcerzak
authors.affiliation: Laboratory of Electron Microscopy, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódż, Poland.
authors.forename: Sława
authors.initials: S
authors.lastname: Glińska
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Miłowska
authors.affiliation: Departamento Química Inorgánica, Universidad de Alcalá de Henares, CIBER-BBN Alcalá de Henares, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Gomez-Ramirez
authors.affiliation: Departamento Química Inorgánica, Universidad de Alcalá de Henares, CIBER-BBN Alcalá de Henares, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: de la Mata
authors.affiliation: Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus.
authors.forename: Dzmitry
authors.initials: D
authors.lastname: Shcharbin
authors.affiliation: Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia.
authors.forename: Iveta
authors.initials: I
authors.lastname: Waczulikova
authors.affiliation: Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Bryszewska
authors.affiliation: Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia.
authors.forename: Tibor
authors.initials: T
authors.lastname: Hianik
mesh_terms: D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D005454:Fluorescence Polarization; D015497:HIV-1 / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism*; D008081:Liposomes; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D012821:Silanes / Q000737:chemistry / Q000378:metabolism*
chemical_list: D050091:Dendrimers; D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010446:Peptide Fragments; D010743:Phospholipids; D012821:Silanes; C504072:carbosilane
keywords: Carbosilane dendrimer; HIV-peptide carrier; Model lipid membranes; Nano-complex