title: Cytokine production suppression by culture supernatant of B16F10 cells and amelioration by Ganoderma lucidum polysaccharides in activated lymphocytes.
abstract: Some cytokines, such as interleukin-2 (IL-2), interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α), produced by lymphocytes might play an important role in anti-tumor immunity and their production is possibly suppressed by cancer. Amelioration of the suppression of cytokine production might contribute to cancer control. Ganoderma lucidum polysaccharides (Gl-PS), a versatile group of a component of G. lucidum and one with various bioactivities, might have this potential. In this study, analyses including reverse transcription and the polymerase chain reaction (RT-PCR), immunocytochemistry and Western blot were used to test the effects of Gl-PS on the production of IL-2, IFN-γ and TNF-α in mononuclear lymphocytes by incubating Gl-PS with mouse splenic mononuclear lymphocytes in the presence of B16F10 cell culture supernatant following activation by phytohemagglutinin. The RT-PCR, immunocytochemistry and Western blot assays showed that the production of IL-2, IFN-γ and TNF-α in mononuclear lymphocytes was suppressed by B16F10 cell culture supernatant at both the mRNA and protein levels, whereas the suppression was fully or partially ameliorated by Gl-PS. The amelioration by Gl-PS against the suppression of the production of IL-2, IFN-γ and TNF-α in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control.
authors.affiliation: The Affiliated Hospital of Chengde Medical College, Chengde, 067000, Hebei Province, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Sun
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Li
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Lin
authors.forename: Xin-Suo
authors.initials: XS
authors.lastname: Duan
authors.forename: En-Hong
authors.initials: EH
authors.lastname: Xing
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Jiang
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Hai-Hua
authors.initials: HH
authors.lastname: Qi
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Niu
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000276:immunology*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology*; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D051379:Mice; D020940:Reishi / Q000737:chemistry*
chemical_list: D016207:Cytokines; D062610:Fungal Polysaccharides