title: Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering.
abstract: In this study, fibrous scaffolds based on poly(γ-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units; and second, modification with the short peptide sequence azidopentanoyl-GGGRGDSGGGY-NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10-3-10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2and 200 ± 100 fm/cm2RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs. Copyright © 2015 John Wiley & Sons, Ltd.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06, Prague 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Svobodová
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06, Prague 6, Czech Republic.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Proks
authors.affiliation: Yeditepe University, Department of Genetics and Bioengineering, 34755, Istanbul, Turkey.
authors.forename: Özge
authors.initials: Ö
authors.lastname: Karabiyik
authors.affiliation: Yeditepe University, Department of Genetics and Bioengineering, 34755, Istanbul, Turkey.
authors.forename: Ayse Ceren
authors.initials: AC
authors.lastname: Çalıkoğlu Koyuncu
authors.affiliation: Yeditepe University, Department of Genetics and Bioengineering, 34755, Istanbul, Turkey.|BIOMATEN Centre of Excellence in Biomaterials and Tissue Engineering, METU, Ankara, Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Torun Köse
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06, Prague 6, Czech Republic.
authors.forename: František
authors.initials: F
authors.lastname: Rypáček
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Square 2, 162 06, Prague 6, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Studenovská
mesh_terms: D000293:Adolescent; D001593:Benzyl Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D002356:Cartilage / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D057930:Click Chemistry; D005971:Glutamates / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014083:Tooth Germ / Q000166:cytology
chemical_list: D001593:Benzyl Compounds; D005971:Glutamates; D006025:Glycosaminoglycans; D010455:Peptides; C582972:poly-(gamma-benzyl-L-glutamate)
keywords: HTGSC; adhesion peptide; fibrous scaffolds; poly(amino acid); radio-assay