title: miR-145 inhibits invasion and metastasis by directly targeting Smad3 in nasopharyngeal cancer.
abstract: MicroRNA-145 (miR-145) has been implicated in several cancers. However, its role in nasopharyngeal carcinoma (NPC) remains unclear. In this study, we proved that miR-145 was significantly downregulated in NPC and associated with NPC cell metastasis. Moreover, miR-145 suppressed Smad3 by directly binding to the 3'-untranslated region (UTR) of Smad3. Knockdown of Smad3 in NPC cells inhibited cell migration and invasion, which was consistent with the effect of miR-145 in NPC cells. In addition, Smad3 expression was inversely correlated with miR-145 level in clinical NPC samples. Taken together, our findings indicate that miR-145 is a tumour suppressor that affects invasive and metastatic properties of NPC via the miR-145/Smad3 axis, leading us to propose that miR-145 overexpression might be a potential therapeutic strategy of NPC intervention.
authors.affiliation: Department of ENT, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou, 215006, China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Huang
authors.forename: Peng
authors.initials: P
authors.lastname: Sun
authors.forename: Zhe
authors.initials: Z
authors.lastname: Lei
authors.forename: Manyi
authors.initials: M
authors.lastname: Li
authors.forename: Yihong
authors.initials: Y
authors.lastname: Wang
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Zhang
authors.forename: Jisheng
authors.initials: J
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D051900:Smad3 Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; C497790:SMAD3 protein, human; D051900:Smad3 Protein