title: Association analysis between g.18873C>T and g.27522G>A genetic polymorphisms of OPG and bone mineral density in Chinese postmenopausal women.
abstract: Several studies report that the OPG is an important candidate gene in the pathogenesis of osteoporosis. This study aimed to detect the potential association of OPG gene polymorphisms with osteoporosis in postmenopausal women. We recruited 928 subjects containing 463 with primary postmenopausal osteoporosis and 465 healthy volunteers as controls. The BMD of neck hip, lumbar spine (L(2-4)), and total hip were assessed by dual-energy X-ray absorptiometry (DEXA). Through the created restriction site-polymerase chain reaction (CRS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), and DNA sequencing methods, the g.18873C>T and g.27522G>A have been investigated. As for g.18873C>T, our data indicated that subjects with CC genotype have significantly higher BMD value than those of CT and TT genotypes (all P values < 0.05). As for g.27522G>A, the BMD values of subjects with GG genotype were significantly higher than those of GA and AA genotypes (all P values < 0.05). Our findings suggest that the OPG g.18873C>T and g.27522G>A genetic polymorphisms are associated with the decreased risk for osteoporosis in Chinese postmenopausal women.
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Endocrinology, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Shan
authors.affiliation: Department of Orthopaedic Surgery, The General Hospital of Beijing Military Area Command, No. 5 Nanmencang Road, Dongcheng District, Beijing 100700, China.
authors.forename: Tianlin
authors.initials: T
authors.lastname: Wen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People; D015519:Bone Density; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D015663:Osteoporosis, Postmenopausal / Q000235:genetics* / Q000503:physiopathology; D053244:Osteoprotegerin / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: D053244:Osteoprotegerin