title: Cervicovaginal cytology in patients undergoing pelvic radiotherapy using the Focalpoint system: results from the RODEO study.
abstract: BACKGROUND
  Evaluate the performance of the Focalpoint system in identifying and classifying cervical cytology alterations from samples collected from patients treated with Radiotherapy (RT).
  
  
  METHODS
  The reproducibility of manual and automated screening by cytotechnologists using the BD Focalpoint GS Imaging System was examined. Samples were collected from May 2010 to August 2011.
  
  
  RESULTS
  A total of 378 treated with RT and 8,967 cytology samples from patients without previous RT, were evaluated. The kappa values for cytological diagnoses read manually and automated in cases without previous RT were as follows: < ASC-H vs. ≥ ASC-H = 0.71; < LSIL vs. ≥ LSIL = 0.66; and < HSIL vs. ≥ HSIL = 0.67. The kappa for cytological diagnoses in post-RT women have showed: < ASC-H vs. ≥ ASC-H = 0.71; < LSIL vs. ≥ LSIL = 0.65; < HSIL vs. ≥ HSIL = 0.57.
  
  
  CONCLUSIONS
  There was no significant difference among the kappa values we found. Post-RT cytology showed small diagnostic agreement between manual and automated examination.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2014_231.
authors.affiliation: Pathology Department, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. mairastein@hotmail.com.
authors.forename: Maíra Degiovani
authors.initials: MD
authors.lastname: Stein
authors.affiliation: Pathology Department, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. mdfregnani@terra.com.br.|Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. mdfregnani@terra.com.br.
authors.forename: José Humberto T G
authors.initials: JH
authors.lastname: Fregnani
authors.affiliation: Pathology Department, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. cristovamscapula@uol.com.br.|Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. cristovamscapula@uol.com.br.
authors.forename: Cristovam
authors.initials: C
authors.lastname: Scapulatempo-Neto
authors.affiliation: Molecular Oncology Research Center, Barretos Cancer Hospital, Pio XII Foundation, Barretos, Brazil. longatto16@hotmail.com.|Laboratory of Medical Investigation (LIM) 14, Faculty of Medicine, University of São Paulo, 01246-903, São Paulo, Brazil. longatto16@hotmail.com.|Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal. longatto16@hotmail.com.|ICVS/3B's - PT Government Associate Laboratory, Braga, Guimarães, Portugal. longatto16@hotmail.com.
authors.forename: Adhemar
authors.initials: A
authors.lastname: Longatto-Filho
mesh_terms: D057205:Automation, Laboratory; D002584:Cervix Uteri / Q000473:pathology* / Q000528:radiation effects*; D004867:Equipment Design; D005260:Female; D005833:Genital Neoplasms, Female / Q000473:pathology* / Q000532:radiotherapy*; D006801:Humans; D008297:Male; D065006:Papanicolaou Test / Q000295:instrumentation*; D011237:Predictive Value of Tests; D011859:Radiography; D011878:Radiotherapy / Q000009:adverse effects; D015203:Reproducibility of Results; D014621:Vagina / Q000000981:diagnostic imaging* / Q000473:pathology*; D014626:Vaginal Smears