title: Engineering of Escherichia coli for the biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from glucose.
abstract: The copolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) [P(HB-co-HHx)] has the potential to serve as a biodegradable tissue engineering material. However, the production of this kind of copolymer still suffers from high cost and uncertainty. We describe here the design of metabolic pathways to synthesize P(HB-co-HHx) directly from glucose using recombinant Escherichia coli. By combining the BktB-dependent condensation pathway with the inverted β-oxidation cycle pathway, we were able to synthesize a P(HB-co-HHx) copolymer with a 10 mol% HHx fraction in recombinant E. coli. After optimizing the host strain and employing thioesterase mutant strains, the engineered E. coli produced 12.9 wt% P(HB-co-HHx) with a 13.2 mol% 3HHx fraction.
authors.affiliation: National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Luan
authors.forename: Xuelian
authors.initials: X
authors.lastname: Cheng
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhuang
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Qi
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis*; D053898:Biosynthetic Pathways; D002208:Caproates; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D060847:Metabolic Engineering; D010957:Plasmids / Q000235:genetics; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D002208:Caproates; D003470:Culture Media; D017931:DNA Primers; D004269:DNA, Bacterial; D005947:Glucose; C115940:poly(3-hydroxybutyrate-co-3-hydroxyhexanoate); D000094122:Polyhydroxybutyrates