title: Glassy-state stabilization of a dominant negative inhibitor anthrax vaccine containing aluminum hydroxide and glycopyranoside lipid A adjuvants.
abstract: During transport and storage, vaccines may be exposed to temperatures outside of the range recommended for storage, potentially causing efficacy losses. To better understand and prevent such losses, dominant negative inhibitor (DNI), a recombinant protein antigen for a candidate vaccine against anthrax, was formulated as a liquid and as a glassy lyophilized powder with the adjuvants aluminum hydroxide and glycopyranoside lipid A (GLA). Freeze-thawing of the liquid vaccine caused the adjuvants to aggregate and decreased its immunogenicity in mice. Immunogenicity of liquid vaccines also decreased when stored at 40°C for 8 weeks, as measured by decreases in neutralizing antibody titers in vaccinated mice. Concomitant with efficacy losses at elevated temperatures, changes in DNI structure were detected by fluorescence spectroscopy and increased deamidation was observed by capillary isoelectric focusing (cIEF) after only 1 week of storage of the liquid formulation at 40°C. In contrast, upon lyophilization, no additional deamidation after 4 weeks at 40°C and no detectable changes in DNI structure or reduction in immunogenicity after 16 weeks at 40°C were observed. Vaccines containing aluminum hydroxide and GLA elicited higher immune responses than vaccines adjuvanted with only aluminum hydroxide, with more mice responding to a single dose.
authors.affiliation: Department of Chemical and Biological Engineering, Center for Pharmaceutical Biotechnology, University of Colorado, Boulder, Boulder, Colorado, 80303.
authors.forename: Kimberly J
authors.initials: KJ
authors.lastname: Hassett
authors.forename: David J
authors.initials: DJ
authors.lastname: Vance
authors.forename: Nishant K
authors.initials: NK
authors.lastname: Jain
authors.forename: Neha
authors.initials: N
authors.lastname: Sahni
authors.forename: Lilia A
authors.initials: LA
authors.lastname: Rabia
authors.forename: Megan C
authors.initials: MC
authors.lastname: Cousins
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Joshi
authors.forename: David B
authors.initials: DB
authors.lastname: Volkin
authors.forename: C Russell
authors.initials: CR
authors.lastname: Middaugh
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Mantis
authors.forename: John F
authors.initials: JF
authors.lastname: Carpenter
authors.forename: Theodore W
authors.initials: TW
authors.lastname: Randolph
mesh_terms: D000277:Adjuvants, Pharmaceutic / Q000737:chemistry* / Q000378:metabolism; D000536:Aluminum Hydroxide / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D022122:Anthrax Vaccines / Q000737:chemistry* / Q000378:metabolism; D004355:Drug Stability; D005260:Female; D005612:Freeze Drying / Q000379:methods; D005615:Freezing; D005898:Glass; D008050:Lipid A / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000277:Adjuvants, Pharmaceutic; D022122:Anthrax Vaccines; D008050:Lipid A; D000536:Aluminum Hydroxide
keywords: aluminum; anthrax; dominant negative inhibitor; formulation; freeze-thaw; glycopyranoside lipid A (GLA); lyophilization; stability; vaccine adjuvants; vaccines