title: The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro.
abstract: BACKGROUND
  For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be the most pre-dominant lactobacilli. In this study, the adhesion ability of LAB20 to intestinal epithelial cell (IECs) lines, IECs isolated from canine intestinal biopsies, and to canine, porcine and human intestinal mucus was investigated. Further, we studied the ability of LAB20 to fortify the epithelial cell monolayer and to reduce LPS-induced interleukin (IL-8) release from enterocytes.
  
  
  RESULTS
  We found that LAB20 presented higher adhesion to canine colonic mucus as compared to mucus isolated from porcine colon. LAB20 showed adhesion to HT-29 and Caco-2 cell lines, and importantly also to canine IECs isolated from canine intestinal biopsies. In addition, LAB20 increased the transepithelial electrical resistance (TER) of enterocyte monolayers and thus strengthened the intestinal barrier function. The strain showed also anti-inflammatory capacity in being able to attenuate the LPS-induced IL-8 production of HT-29 cells.
  
  
  CONCLUSIONS
  In conclusion, canine indigenous strain LAB20 is a potential probiotic candidate for dogs adhering to the host epithelium and showing intestinal barrier fortifying and anti-inflammatory effects.
authors.affiliation: Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66 (Agnes Sjöberginkatu 2), FI-00014, Helsinki, Finland. veera.kainulainen@helsinki.fi.
authors.forename: Veera
authors.initials: V
authors.lastname: Kainulainen
authors.affiliation: Department of Food and Environmental Sciences, Faculty of Agriculture and Forestry, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FI-00014, Helsinki, Finland. yurui.tang@helsinki.fi.
authors.forename: Yurui
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), FI-00014, Helsinki, Finland. thomas.spillmann@helsinki.fi.
authors.forename: Thomas
authors.initials: T
authors.lastname: Spillmann
authors.affiliation: Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57 (Viikintie 49), FI-00014, Helsinki, Finland. susanne.kilpinen@helsinki.fi.
authors.forename: Susanne
authors.initials: S
authors.lastname: Kilpinen
authors.affiliation: Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66 (Agnes Sjöberginkatu 2), FI-00014, Helsinki, Finland. justus.reunanen@helsinki.fi.
authors.forename: Justus
authors.initials: J
authors.lastname: Reunanen
authors.affiliation: Department of Food and Environmental Sciences, Faculty of Agriculture and Forestry, University of Helsinki, P.O. Box 56 (Viikinkaari 9), FI-00014, Helsinki, Finland. per.saris@helsinki.fi.
authors.forename: Per E J
authors.initials: PE
authors.lastname: Saris
authors.affiliation: Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 66 (Agnes Sjöberginkatu 2), FI-00014, Helsinki, Finland. reetta.satokari@helsinki.fi.
authors.forename: Reetta
authors.initials: R
authors.lastname: Satokari
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D002478:Cells, Cultured; D004285:Dogs; D020895:Enterocytes / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000382:microbiology*; D007583:Jejunum / Q000382:microbiology; D007779:Lactobacillus acidophilus / Q000302:isolation & purification / Q000502:physiology*; D009093:Mucus / Q000378:metabolism / Q000382:microbiology
chemical_list: D016209:Interleukin-8