title: Fatty acid changes help to better understand regression of nonalcoholic fatty liver disease.
abstract: OBJECTIVE
  To investigate whether liver steatosis reduction due to a six-month dietary intervention results in significant changes in the concentrations of fatty acids.
  
  
  METHODS
  A group of 35 Caucasian individuals diagnosed with different levels of steatosis were prospectively enrolled in the present study. Analysis of the fatty acid profiles was performed according to changes in liver steatosis (liver steatosis reduction by one or two degrees) after a six-month dietary intervention. The diet helped reduce body mass in obese and overweight patients, and stabilize both glycemia and dyslipidemia. Fatty acids were extracted according to the Folch method and analyzed by gas chromatography.
  
  
  RESULTS
  This study showed significant changes in fatty acid profiles in patients who had reduced liver steatosis by one as well as two degrees. A reduction in liver steatosis by one degree caused a significant increase in the level of the n-3 family: eicosapentaenoic acid (P < 0.055), docosapentaenoic acid-C 22:5 (P < 0.05) and docosahexaenoic acid (P < 0.05). A reduction in liver steatosis by two degrees caused a significant decrease in serum palmitoleic acid-C 16:1 (P < 0.05).
  
  
  CONCLUSIONS
  Liver steatosis reduction is associated with changes in fatty acid profiles, and these changes may reflect an alteration in fatty acid synthesis and metabolism. These findings may help better understand regression of nonalcoholic fatty liver disease.
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Maciejewska
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Arleta
authors.initials: A
authors.lastname: Drozd
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Ossowski
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Karina
authors.initials: K
authors.lastname: Ryterska
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Jamioł-Milc
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Banaszczak
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Raszeja-Wyszomirska
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kaczorowska
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Sabinicz
authors.affiliation: Dominika Maciejewska, Arleta Drozd, Piotr Ossowski, Karina Ryterska, Dominika Jamioł-Milc, Marcin Banaszczak, Małgorzata Kaczorowska, Anna Sabinicz, Ewa Stachowska, Department of Biochemistry and Human Nutrition, Pomeranian Medical University, 70-204 Szczecin, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Stachowska
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage* / Q000097:blood; D004281:Docosahexaenoic Acids / Q000008:administration & dosage / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000097:blood; D005227:Fatty Acids / Q000008:administration & dosage* / Q000097:blood; D005229:Fatty Acids, Monounsaturated / Q000008:administration & dosage / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000175:diagnosis / Q000178:diet therapy* / Q000208:ethnology; D011044:Poland / Q000453:epidemiology; D011446:Prospective Studies; D012074:Remission Induction; D012720:Severity of Illness Index; D013997:Time Factors; D016896:Treatment Outcome; D015431:Weight Loss; D044465:White People; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D004041:Dietary Fats; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; C008757:palmitoleic acid; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; C026219:docosapentaenoic acid
keywords: Biochemical parameters; Diet; Fatty acids; Gas chromatography; Nonalcoholic fatty liver disease; Sterol regulatory element binding proteins 1c; n-3 Family; n-6 Family