title: Structural measurements and cell line studies of the copper-PEG-Rifampicin complex against Mycobacterium tuberculosis.
abstract: The bacterium responsible for tuberculosis is increasing its resistance to antibiotics resulting in new multidrug-resistant Mycobacterium tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB). In this study, several analytical techniques including NMR, FT-ICR, MALDI-MS, LC-MS and UV/Vis are used to study the copper-Rifampicin-Polyethylene glycol (PEG-3350) complex. The copper (II) cation is a carrier for the antibiotic Rifampicin as well as nutrients for the bacterium. The NIH-NIAID cell line containing several Tb strains (including antibiotic resistant strains) is tested against seven copper-PEG-RIF complex variations.
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States. Electronic address: tmanning@valdosta.edu.
authors.forename: Thomas
authors.initials: T
authors.lastname: Manning
authors.affiliation: Department of Chemistry, Valdosta State University, Valdosta, GA 31698, United States.
authors.forename: Rachel
authors.initials: R
authors.lastname: Mikula
authors.affiliation: Nuclear Magnetic Resonance Facility, Department of Chemistry, University of Georgia, Athens, GA, United States.
authors.forename: Greg
authors.initials: G
authors.lastname: Wylie
authors.affiliation: PAMS, Department of Chemistry, University of Georgia, Athens, GA, United States.
authors.forename: Dennis
authors.initials: D
authors.lastname: Phillips
authors.affiliation: Fourier Transform-Ion Cyclotron Resonance Facility, National High Field Magnet Lab, Tallahassee, FL, United States.
authors.forename: Jackie
authors.initials: J
authors.lastname: Jarvis
authors.affiliation: NMR Facility, National High Field Magnet Lab, Tallahassee, FL, United States.
authors.forename: Fengli
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology; D003300:Copper / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D012293:Rifampin / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000995:Antitubercular Agents; D011092:Polyethylene Glycols; D003300:Copper; D012293:Rifampin
keywords: Antibiotic; Drug delivery; FT-ICR; Mass Spec; Mycobacterium tuberculosis; NMR; RIF; Tb