title: Production of fermentable sugars from sugarcane bagasse by enzymatic hydrolysis after autohydrolysis and mechanical refining.
abstract: The autohydrolysis process has been considered a simple, low-cost and environmental friendly technology for generation of sugars from biomass. In order to improve accessibility of enzymes during enzymatic hydrolysis as well as to allow the recovery of hemicellulose in the filtrate, the sugarcane bagasse was pretreated using autohydrolysis followed by a mechanical refining process. The autohydrolysis was carried out in three different conditions. Autohydrolysis at 190°C for 10min provided the highest overall sugar (19.2/100g raw bagasse) in prehydrolyzate. The enzymatic hydrolysis step was performed for all the post-treated solids with and without refining at enzyme loadings of 5 and 10FPU/g for 96h. A total of 84.4% of sugar can be recovered from sugarcane bagasse at 180°C for 20min with 5 FPU/g enzyme charge. The economic analysis for the proposed method showed that the bioethanol production can have a financial return larger than 12%.
authors.affiliation: Department of Chemistry at Federal University of Viçosa, Viçosa, MG 36570-000, Brazil. Electronic address: larisse.batalha@ufv.br.
authors.forename: Larisse Aparecida Ribas
authors.initials: LA
authors.lastname: Batalha
authors.affiliation: Department of Forestry Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Han
authors.affiliation: Department of Forestry Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Hasan
authors.initials: H
authors.lastname: Jameel
authors.affiliation: Department of Forestry Biomaterials, North Carolina State University, Campus Box 8005, Raleigh, NC 27695-8005, USA.
authors.forename: Hou-Min
authors.initials: HM
authors.lastname: Chang
authors.affiliation: Department of Forestry Engineering at Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Jorge Luiz
authors.initials: JL
authors.lastname: Colodette
authors.affiliation: Department of Forestry Engineering at Federal University of Viçosa, Viçosa, MG 36570-000, Brazil.
authors.forename: Fernando José
authors.initials: FJ
authors.lastname: Borges Gomes
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D002241:Carbohydrates / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; C027433:bagasse
keywords: Autohydrolysis; Enzymatic hydrolysis; Fermentable sugar; Refining; Sugarcane bagasse