title: Role of indirect readout mechanism in TATA box binding protein-DNA interaction.
abstract: Gene expression generally initiates from recognition of TATA-box binding protein (TBP) to the minor groove of DNA of TATA box sequence where the DNA structure is significantly different from B-DNA. We have carried out molecular dynamics simulation studies of TBP-DNA system to understand how the DNA structure alters for efficient binding. We observed rigid nature of the protein while the DNA of TATA box sequence has an inherent flexibility in terms of bending and minor groove widening. The bending analysis of the free DNA and the TBP bound DNA systems indicate presence of some similar structures. Principal coordinate ordination analysis also indicates some structural features of the protein bound and free DNA are similar. Thus we suggest that the DNA of TATA box sequence regularly oscillates between several alternate structures and the one suitable for TBP binding is induced further by the protein for proper complex formation.
authors.affiliation: Computational Science Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India.
authors.forename: Manas
authors.initials: M
authors.lastname: Mondal
authors.forename: Devapriya
authors.initials: D
authors.lastname: Choudhury
authors.forename: Jaydeb
authors.initials: J
authors.lastname: Chakrabarti
authors.forename: Dhananjay
authors.initials: D
authors.lastname: Bhattacharyya
mesh_terms: D001665:Binding Sites; D056004:Molecular Dynamics Simulation; D009690:Nucleic Acid Conformation; D025341:Principal Component Analysis; D055550:Protein Stability; D016385:TATA Box; D035181:TATA-Box Binding Protein / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D035181:TATA-Box Binding Protein