title: In vivo nonlinear spectral imaging as a tool to monitor early spectroscopic and metabolic changes in a murine cutaneous squamous cell carcinoma model.
abstract: Timely detection of cutaneous squamous cell carcinoma with non-invasive modalities like nonlinear spectral imaging (NLSI) can ensure efficient preventive or therapeutic measures for patients. In this study, in vivo NLSI was used to study spectral characteristics in murine skin treated with 7, 12-dimethylbenz(a)anthracene. The results show that NLSI could detect emission spectral changes during the early preclinical stages of skin carcinogenesis. Analyzing these emission spectra using simulated band-pass filters at 450-460 nm and 525-535 nm, gave parameters that were expressed as a ratio. This ratio was increased and thus suggestive of elevated metabolic activity in early stages of skin carcinogenesis.
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Centre, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands ; Centre for Optical Diagnostics and Therapy, Erasmus Medical Centre, Post Box 2040, 3000 CA, Rotterdam, The Netherlands.
authors.forename: Giju
authors.initials: G
authors.lastname: Thomas
authors.affiliation: Department of Molecular Biophysics, Utrecht University, 3508 TA, Utrecht, The Netherlands.
authors.forename: Johan
authors.initials: J
authors.lastname: van Voskuilen
authors.affiliation: Department of Molecular Biophysics, Utrecht University, 3508 TA, Utrecht, The Netherlands.
authors.forename: Hoa
authors.initials: H
authors.lastname: Truong
authors.affiliation: Department of Experimental Animal Pathology, the Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Amsterdam, The Netherlands.
authors.forename: Ji-Ying
authors.initials: JY
authors.lastname: Song
authors.affiliation: Department of Molecular Biophysics, Utrecht University, 3508 TA, Utrecht, The Netherlands.
authors.forename: Hans C
authors.initials: HC
authors.lastname: Gerritsen
authors.affiliation: Department of Biomedical Engineering and Physics, Academic Medical Centre, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.
authors.forename: H J C M
authors.initials: HJ
authors.lastname: Sterenborg
keywords: (170.1870) Dermatology; (170.3880) Medical and biological imaging; (170.6510) Spectroscopy, tissue diagnostics; (180.4315) Nonlinear microscopy; (180.6900) Three-dimensional microscopy; (300.6420) Spectroscopy, nonlinear