title: Metabolic profiling of epithelial ovarian cancer cell lines: evaluation of harvesting protocols for profiling using NMR spectroscopy.
abstract: BACKGROUND
  Metabolic profiling represents a novel technology for analyzing tumor cells. Epithelial ovarian carcinoma has a low survival rate due to the development of aggressive and chemotherapy-resistant cells. A tailored and reliable protocol is presented for profiling of chemoresistant cells using the cell line SKOV3 and a multiresistant subline SKOV3R.
  
  
  RESULTS
  Harvesting protocols with cold methanol or MilliQ freeze/thaw cycles were compared. Increased reproducibility using MilliQ was evidenced. Importantly, both approaches resulted in similar profiles. Compared with parental SKOV3, the SKOV3R cells showed a significantly different profile.
  
  
  CONCLUSIONS
  The MilliQ protocol is preferred owing to higher reproducibility and increased sample preparation options. The resulting metabolic profiles summarize metabolic alterations in chemoresistant cells consistent with a progressed and aggressive phenotype.
authors.affiliation: Division of Analytical Pharmaceutical Chemistry, Uppsala University, Box 574, SE-751 23 Uppsala, Sweden.
authors.forename: Mikael  K R
authors.initials: M
authors.lastname: Engskog
authors.forename: My
authors.initials: M
authors.lastname: Björklund
authors.forename: Jakob
authors.initials: J
authors.lastname: Haglöf
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Arvidsson
authors.forename: Maria
authors.initials: M
authors.lastname: Shoshan
authors.forename: Curt
authors.initials: C
authors.lastname: Pettersson
mesh_terms: D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D016208:Databases, Factual; D019008:Drug Resistance, Neoplasm; D005260:Female; D005615:Freezing; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D055442:Metabolome; D055432:Metabolomics; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D025341:Principal Component Analysis; D015203:Reproducibility of Results