title: Genetic diversity of avian influenza A (H10N8) virus in live poultry markets and its association with human infections in China.
abstract: Following the first human infection with the influenza A (H10N8) virus in Nanchang, China in December 2013, we identified two additional patients on January 19 and February 9, 2014. The epidemiologic, clinical, and virological data from the patients and the environmental specimen collected from 23 local live poultry markets (LPMs) were analyzed. The three H10N8 cases had a history of poultry exposure and presented with high fever (>38°C), rapidly progressive pneumonia and lymphopenia. Substantial high levels of cytokines and chemokines were observed. The sequences from an isolate (A/Environment/Jiangxi/03489/2013 [H10N8]) in an epidemiologically linked LPM showed highly identity with human H10N8 virus, evidencing LPM as the source of human infection. The HA and NA of human and environmental H10N8 isolates showed high identity (99.1-99.9%) while six genotypes with internal genes derived from H9N2, H7N3 and H7N9 subtype viruses were detected in environmental H10N8 isolates. The genotype of the virus causing human infection, Jiangxi/346, possessed a whole internal gene set of the A/Environment/Jiangxi/10618/2014(H9N2)-like virus. Thus, our findings support the notion that LPMs can act as both a gene pool for the generation of novel reassortants and a source for human infection, and intensive surveillance and management should therefore be conducted.
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Mingbin
authors.initials: M
authors.lastname: Liu
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Li
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Hui
authors.initials: H
authors.lastname: Yuan
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Hong
authors.initials: H
authors.lastname: Bo
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Wen
authors.initials: W
authors.lastname: Xia
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Rongbao
authors.initials: R
authors.lastname: Gao
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Guo
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Huang
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zou
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Dayan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: ShiWen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Shengen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Maohong
authors.initials: M
authors.lastname: Hu
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Xiansheng
authors.initials: X
authors.lastname: Ni
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Tian
authors.initials: T
authors.lastname: Gong
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Jianxiong
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangxi Provincial Disease Control and Prevention, Nanchang, China, 330029.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Donghu District Center for Disease Control and Prevention, Nanchang, China, 330008.
authors.forename: Jun
authors.initials: J
authors.lastname: Cai
authors.affiliation: Jiangxi Provincial people's Hospital, Nanchang, China, 330006.
authors.forename: Zuke
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: The first affiliated hospital of Nanchang University, Nanchang, China, 330008.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: The fourth affiliated hospital of Nanchang University, China, 330006.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Dexin
authors.initials: D
authors.lastname: Li
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Wu
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Zijian
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Chinese Center for Disease Control and Prevention, Beijing, China, 102206.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Nanchang Center for Disease Control and Prevention, Nanchang, China, 330038.
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
authors.affiliation: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China, 102206.
authors.forename: Yuelong
authors.initials: Y
authors.lastname: Shu
mesh_terms: D000368:Aged; D000818:Animals; D018925:Chemokines / Q000097:blood; D002681:China / Q000453:epidemiology; D016207:Cytokines / Q000097:blood; D005260:Female; D005838:Genotype; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D065926:Influenza A Virus, H10N8 Subtype / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D057920:Influenza A Virus, H7N3 Subtype / Q000235:genetics / Q000302:isolation & purification; D064766:Influenza A Virus, H7N9 Subtype / Q000235:genetics / Q000302:isolation & purification; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000302:isolation & purification; D005585:Influenza in Birds / Q000821:virology*; D007251:Influenza, Human / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics; D010802:Phylogeny; D011200:Poultry
chemical_list: D018925:Chemokines; D016207:Cytokines; D006388:Hemagglutinins; D009439:Neuraminidase