title: Isolation and characterization of an antimicrobial lipopeptide produced by Paenibacillus ehimensis MA2012.
abstract: In this study, a novel lipopeptide antibiotic was isolated from the culture supernatant of Paenibacillus ehimensis strain MA2012. After analyses by mass spectrometry (MS), nuclear magnetic resonance (NMR), and high resolution mass spectrometry (HR-MS/MS) the compound was identified to be polypeptin C consisting of 3-hydroxy-4-methyl-hexanoic acid moiety and nine amino acids as peptide body. It has the same molecular mass (1115 Da) with that of polypeptin A and B but the amino acid positions differ. A relatively low concentration (125 ppm) of polypeptin C lowered the surface tension of water from 72.2 to 36.4 mN/m. It showed antimicrobial activity against several plant pathogenic bacteria and fungi. When the polypeptin C was applied to the ripe pepper fruits previously inoculated with conidia of Colletotrichum gloeosporioides, the hyphal growth on the fruit was significantly suppressed. Moreover, the hyphal morphology of C. gloeosporioides was greatly affected by the purified compound. All these data suggest the great potential of P. ehimensis MA2012 to control plant fungal and bacterial diseases.
authors.affiliation: Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Kyaw Wai
authors.initials: KW
authors.lastname: Naing
authors.affiliation: Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Yong Seong
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Xuan Hoa
authors.initials: XH
authors.lastname: Nguyen
authors.affiliation: Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Min Hae
authors.initials: MH
authors.lastname: Jeong
authors.affiliation: Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Anees
authors.affiliation: Bureau of Agriculture, Lanad and Ocean Policy, Office for Government Policy Coordination, Dasomro, Sejong, Korea.
authors.forename: Byeong Seok
authors.initials: BS
authors.lastname: Oh
authors.affiliation: Department of Food Engineering, Mokpo National University, Mokpo, Korea.
authors.forename: Jeong Yong
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Department of Food Science and Technology and Functional Food Research Center, Chonnam National University, Gwangju, Korea.
authors.forename: Jae Hak
authors.initials: JH
authors.lastname: Moon
authors.affiliation: Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Korea.
authors.forename: Kil Yong
authors.initials: KY
authors.lastname: Kim
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D061046:Biological Control Agents; D020231:Colletotrichum / Q000187:drug effects; D004275:DNA, Ribosomal / Q000737:chemistry; D005658:Fungi / Q000187:drug effects; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D056507:Paenibacillus / Q000378:metabolism*; D011113:Polymyxins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D013500:Surface Tension / Q000187:drug effects; D053719:Tandem Mass Spectrometry; D014867:Water
chemical_list: D000890:Anti-Infective Agents; D061046:Biological Control Agents; D004275:DNA, Ribosomal; D011113:Polymyxins; D012336:RNA, Ribosomal, 16S; D014867:Water; C013652:polypeptin
keywords: Antimicrobial activity; Paenibacillus ehimensis MA2012; Polypeptin C; Surface tension