title: Understanding the biochemical basis of temperature-induced lipid pathway adjustments in plants.
abstract: Glycerolipid biosynthesis in plants proceeds through two major pathways compartmentalized in the chloroplast and the endoplasmic reticulum (ER). The involvement of glycerolipid pathway interactions in modulating membrane desaturation under temperature stress has been suggested but not fully explored. We profiled glycerolipid changes as well as transcript dynamics under suboptimal temperature conditions in three plant species that are distinctively different in the mode of lipid pathway interactions. In Arabidopsis thaliana, a 16:3 plant, the chloroplast pathway is upregulated in response to low temperature, whereas high temperature promotes the eukaryotic pathway. Operating under a similar mechanistic framework, Atriplex lentiformis at high temperature drastically increases the contribution of the eukaryotic pathway and correspondingly suppresses the prokaryotic pathway, resulting in the switch of lipid profile from 16:3 to 18:3. In wheat (Triticum aestivum), an 18:3 plant, low temperature also influences the channeling of glycerolipids from the ER to chloroplast. Evidence of differential trafficking of diacylglycerol moieties from the ER to chloroplast was uncovered in three plant species as another layer of metabolic adaptation under temperature stress. We propose a model that highlights the predominance and prevalence of lipid pathway interactions in temperature-induced lipid compositional changes.
authors.affiliation: National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada.
authors.forename: Wenyun
authors.initials: W
authors.lastname: Shen
authors.affiliation: National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada.
authors.forename: Dustin
authors.initials: D
authors.lastname: Cram
authors.affiliation: Department of Plant Sciences, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5A8, Canada.
authors.forename: D Brian
authors.initials: DB
authors.lastname: Fowler
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors.forename: Yangdou
authors.initials: Y
authors.lastname: Wei
authors.affiliation: National Research Council Canada, Saskatoon, Saskatchewan S7N 0W9, Canada jitao.zou@nrc-cnrc.gc.ca.
authors.forename: Jitao
authors.initials: J
authors.lastname: Zou
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism; D003080:Cold Temperature; D004721:Endoplasmic Reticulum / Q000378:metabolism; D050356:Lipid Metabolism / Q000502:physiology; D010944:Plants / Q000378:metabolism*; D013696:Temperature; D014908:Triticum / Q000378:metabolism