title: Effects of high CO₂ levels on fermentation, peroxidation, and cellular water stress in Fragaria vesca stored at low temperature in conditions of unlimited O₂.
abstract: To better understand the tolerance of strawberries (Fragaria vesca L.) to high CO2 in storage atmospheres, fermentation and cellular damage were investigated. Fruits were stored for 3 and 6 days at 0 °C in the presence of different CO2 levels (0, 20, or 40%) with 20% O2. Changes in pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) gene expression and in fermentative metabolites, as well as in bound water and malondialdehyde (MDA) concentrations, were analyzed. In strawberries stored without added CO2, up-regulation of PDC and ADH was not associated with an increase in fermentative metabolites. By contrast, moderate ethanol fermentation in fruits exposed to 20% CO2 seems to be essential to maintain fruit metabolism, reducing both lipid peroxidation and cellular water stress. However, if the CO2 concentration increases (40%), the excess acetaldehyde and ethanol produced were closely correlated with a decrease in bound water and production of MDA.
authors.affiliation: Department of Characterization, Quality and Security and ‡Department of Metabolism and Nutrition, Institute of Food Science Technology and Nutrition (ICTAN-CSIC) , Madrid, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Blanch
authors.forename: Raquel
authors.initials: R
authors.lastname: Rosales
authors.forename: Raquel
authors.initials: R
authors.lastname: Mateos
authors.forename: María B
authors.initials: MB
authors.lastname: Perez-Gago
authors.forename: Maria T
authors.initials: MT
authors.lastname: Sanchez-Ballesta
authors.forename: María I
authors.initials: MI
authors.lastname: Escribano
authors.forename: Carmen
authors.initials: C
authors.lastname: Merodio
mesh_terms: D000079:Acetaldehyde / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000235:genetics; D002245:Carbon Dioxide / Q000008:administration & dosage*; D003080:Cold Temperature; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects*; D005519:Food Preservation / Q000379:methods*; D031985:Fragaria / Q000378:metabolism*; D005638:Fruit / Q000378:metabolism; D015870:Gene Expression; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis; D011767:Pyruvate Decarboxylase / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D002245:Carbon Dioxide; D000431:Ethanol; D008315:Malondialdehyde; D000426:Alcohol Dehydrogenase; D011767:Pyruvate Decarboxylase; D000079:Acetaldehyde
keywords: MDA; acetaldehyde; bound water; ethanol; fermentative genes; high CO2 levels