title: Effect of melatonin on antioxidant status and circadian activity rhythm during hepatocarcinogenesis in mice.
abstract: OBJECTIVE
  Alteration of circadian systems can cause cancer and affects its development and response to therapeutics. The present study investigates whether cancer can disrupt circadian locomotor rhythms and evaluated the influence of melatonin (MLT) and oxaliplatin on the levels of antioxidants and circadian locomotor activity rhythms in N-nitrosodiethylamine (NDEA)-induced liver tumor in Indian field mouse (Mus booduga).
  
  
  METHODS
  Effects of NDEA, NDEA, and MLT, as well as NDEA and oxaliplatin, on levels of mice liver marker enzymes and antioxidants and their circadian locomotor activity rhythm were assessed.
  
  
  RESULTS
  Treatment of mice with NDEA caused significant alteration of their liver marker enzymes [aspartate transaminase and alanine transaminase; P<0.05 Duncan's multiple range test (DMRT) test] antioxidant levels (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase; P<0.05, DMRT test] and circadian locomotor activity rhythm, which were abrogated when the animals were also given MLT or the anticancer drug, oxaliplatin.
  
  
  CONCLUSIONS
  Our study demonstrated that the circadian clock was disturbed by hepatocarcinogenesis and the effects could be reversed by the chronobiotic, MLT.
authors.forename: Devi
authors.initials: D
authors.lastname: Verma
authors.forename: Onn Haji
authors.initials: OH
authors.lastname: Hashim
authors.forename: Jaime Jacqueline
authors.initials: JJ
authors.lastname: Jayapalan
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India, .
authors.forename: Perumal
authors.initials: P
authors.lastname: Subramanian
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D063646:Carcinogenesis; D002374:Catalase / Q000378:metabolism; D002940:Circadian Rhythm / Q000187:drug effects*; D004052:Diethylnitrosamine / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000517:prevention & control; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D009944:Organoplatinum Compounds / Q000008:administration & dosage; D000077150:Oxaliplatin; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009944:Organoplatinum Compounds; D000077150:Oxaliplatin; D004052:Diethylnitrosamine; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D008550:Melatonin