title: KAP1 is a Novel Substrate for the Arginine Methyltransferase PRMT5.
abstract: KRAB-associated protein 1 (KAP1), the transcriptional corepressor of Kruppel-associated box zinc finger proteins (KRAB-ZFPs), is subjected to multiple post-translational modifications that are involved in fine-tuning of the multiple biological functions of KAP1. In previous papers, we analyzed the KAP1-dependent molecular mechanism of transcriptional repression mediated by ZNF224, a member of the KRAB-ZFP family, and identified the protein arginine methyltransferase PRMT5 as a component of the ZNF224 repression complex. We demonstrated that PRMT5-mediated histone arginine methylation is required to elicit ZNF224 transcriptional repression. In this study, we show that KAP1 interacts with PRMT5 and is a novel substrate for PRMT5 methylation. Also, we present evidence that the methylation of KAP1 arginine residues regulate the KAP1-ZNF224 interaction, thus suggesting that this KAP1 post-translational modification could actively contribute to the regulation of ZNF224-mediated repression.
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via S. Pansini 5, Naples 80131, Italy. rob.dicaprio@libero.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: di Caprio
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via S. Pansini 5, Naples 80131, Italy. michelaciano@libero.it.
authors.forename: Michela
authors.initials: M
authors.lastname: Ciano
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via S. Pansini 5, Naples 80131, Italy. giorgia.montano@unina.it.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Montano
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via S. Pansini 5, Naples 80131, Italy. paola.costanzo@unina.it.
authors.forename: Paola
authors.initials: P
authors.lastname: Costanzo
authors.affiliation: Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via S. Pansini 5, Naples 80131, Italy. elenacesaro@virgilio.it.
authors.forename: Elena
authors.initials: E
authors.lastname: Cesaro