title: Cardiopulmonary bypass changes the plasma proteome in children undergoing tetralogy of Fallot repair.
abstract: BACKGROUND
  Cardiopulmonary bypass (CPB) can be associated with deleterious clinical effects. However, the impact of CPB on inflammatory, immunological and other homeostatic pathways remains poorly understood. We investigated the impact of CPB on the plasma proteome in children undergoing tetralogy of Fallot repair.
  
  
  METHODS
  Blood samples were taken from 20 children prior to and at the end of CPB and 6h, 12h and 24h after CPB. Plasma was analysed by liquid chromatography-mass spectrometry (LC-MS) in a label-free, untargeted approach. Data were analysed using Genedata software to identify peptides that were differentially expressed (p<0.01 above a false discovery rate). Proteins were identified from peptides that demonstrated differential expression.
  
  
  RESULTS
  The proteins that were found to be differentially expressed were haptoglobin isoform 1 preproprotein, isoform 2 of semaphorin-6C, vitamin D-binding protein, inter-alpha-trypsin inhibitor, ceruloplasmin, apolipoprotein B100 and fibrinogen alpha.
  
  
  CONCLUSIONS
  CPB alters the plasma proteome with differences most apparent at 6h and 12h post CPB. There was a return to baseline with no proteins differentially regulated by 24h.
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia Cardiac Surgery Unit, Royal Children's Hospital, Melbourne, Australia Department of Paediatrics, The University of Melbourne, Melbourne, Australia Department of Primary Industries, Bioscience Research Division, Melbourne, Australia.
authors.forename: M
authors.initials: M
authors.lastname: Hepponstall
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Ignjatovic
authors.affiliation: Department of Primary Industries, Bioscience Research Division, Melbourne, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Binos
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: C
authors.initials: C
authors.lastname: Attard
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia.
authors.forename: V
authors.initials: V
authors.lastname: Karlaftis
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia Cardiac Surgery Unit, Royal Children's Hospital, Melbourne, Australia.
authors.forename: Y
authors.initials: Y
authors.lastname: d'Udekem
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia Department of Paediatrics, The University of Melbourne, Melbourne, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Monagle
authors.affiliation: Murdoch Childrens Research Institute, Melbourne, Australia Cardiac Surgery Unit, Royal Children's Hospital, Melbourne, Australia Department of Paediatrics, The University of Melbourne, Melbourne, Australia igor.konstantinov@rch.org.au.
authors.forename: I E
authors.initials: IE
authors.lastname: Konstantinov
mesh_terms: D002315:Cardiopulmonary Bypass; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D008297:Male; D020543:Proteome / Q000378:metabolism*; D013771:Tetralogy of Fallot / Q000097:blood* / Q000601:surgery*
chemical_list: D020543:Proteome
keywords: cardiac surgery; cardiopulmonary bypass; mass spectrometry; paediatric; proteins; proteomics