title: H-NS is a repressor of major virulence gene loci in Vibrio parahaemolyticus.
abstract: Vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci T3SS1, Vp-PAI (T3SS1+tdh2) and T6SS2. As showing in this study, the nucleoid-associated DNA-binding regulator H-NS binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover H-NS inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of V. parahaemolyticus. H-NS appears to act as a major repressor of the virulence of this pathogen. Date presented here would promote us to gain a deeper understanding of H-NS-mediated silencing of horizontally acquired virulence loci in V. parahaemolyticus.
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China ; School of Medicine, Jiangsu University Zhenjiang, China.
authors.forename: Yiquan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Center, Academy of Military Medical Sciences Beijing, China.
authors.forename: Yefeng
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University Chongqing, China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Department of Biosafety, Beijing Institute of Microbiology and Epidemiology Beijing, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
keywords: H-NS; T3SS1; T6SS2; Vibrio parahaemolyticus; Vp-PAI