title: [IL-35 level of peripheral blood is correlated with the immune response to hepatitis B vaccine].
abstract: OBJECTIVE
  To investigate the relationship of IL-35 level in peripheral blood mononuclear cells (PBMCs) and immune response to hepatitis B vaccine and explore the factors influencing vaccine immune reaction.
  
  
  METHODS
  Our follow-up study enrolled 400 healthy volunteers who had received a standard course of immunization with hepatitis B vaccine. Chemiluminescence method was carried out to quantitatively detect hepatitis B virus surface antibody (HBsAb). PBMCs were isolated from 8 cases of non- or hypo-responders and 8 cases of moderate or hyper-responders, and the total RNA was extracted. Real-time quantitative PCR was performed to detect Epstein-Barr virus (EBV)-induced gene 3 (EBI3) and IL-12p35 mRNA levels. Finally, serum IL-35 level was compared using ELISA between 40 cases of non- or hypo-responders and 40 cases of moderate or hyper-responders randomly selected from the healthy volunteers to analyze the association with HBsAb level.
  
  
  RESULTS
  The levels of EBI3 and IL-12p35 mRNAs in PBMCs in moderate or hyper-responders were significantly higher than those in non- or hypo-responders, and the former was (12.73 ± 2.26) and (7.93 ± 1.06) folds the latter, respectively. The serum IL-35 level in moderate or hyper-responders [(111.50 ± 49.53) ng/L] was significantly higher than that in non-or hypo-responders [(15.01 ± 12.82) ng/L]; and IL-35 level was positively correlated with HBsAb level (r=0.84).
  
  
  CONCLUSIONS
  Peripheral blood IL-35 level was closely correlated with the immune response to hepatitis B vaccine and low IL-35 level might be one of possible reasons or mechanisms underlying non-response or hypo-response to hepatitis B vaccine immunization.
authors.affiliation: Department of Clinical Laboratory, Nanjing Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Department of Clinical Laboratory, Nanjing Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Junhao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Nanjing Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Qingfei
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Nanjing Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Hospital of Nanjing University, Nanjing 210008, China.
authors.forename: Ronghai
authors.initials: R
authors.lastname: Fang
authors.affiliation: Pukou District Yongning Healthcare, Nanjing 211801, China.
authors.forename: Xihua
authors.initials: X
authors.lastname: Nie
authors.affiliation: Lishui People's Hospital, Nanjing 211200, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Nanjing Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Guangyi
authors.initials: G
authors.lastname: Gu
mesh_terms: D000328:Adult; D005260:Female; D005500:Follow-Up Studies; D006509:Hepatitis B / Q000097:blood* / Q000276:immunology / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007378:Interleukins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D014761:Viral Hepatitis Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D055815:Young Adult
chemical_list: D006510:Hepatitis B Antibodies; D007378:Interleukins; D014761:Viral Hepatitis Vaccines; C532417:interleukin-35, human