title: Identification of a marine Bacillus strain C5 and parathion-methyl degradation characteristics of the extracellular esterase B1.
abstract: A bacterial strain C5 that can produce new type of marine esterase was isolated and screened from marine sludge. According to 16S rRNA sequence analysis and physiological and biochemical experiments, the strain was identified as Bacillus subtilis. A single isozyme with a molecular weight of 86 kDa was observed by SDS-PAGE and native-PAGE. On this basis, the mechanism of esterase B1 secreted by strain C5 degrading parathion-methyl was explored, and the effects of temperature and pH on the degradation rate were investigated. From the results, p-nitrophenol was one of the degradation products of B1 degrading parathion-methyl, and the best degradation effect could be achieved at the temperature of 40°C and the neutral pH value.
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
authors.forename: Junzhong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, Shandong 266071, China.
authors.forename: Mi
authors.initials: M
authors.lastname: Sun
mesh_terms: D059001:Aquatic Organisms / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology* / Q000302:isolation & purification; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008743:Methyl Parathion / Q000737:chemistry; D010802:Phylogeny; D059748:Proteolysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S; D008743:Methyl Parathion; D012697:Serine Endopeptidases; C049262:esterase B