title: Coadministration of basic fibroblast growth factor-loaded polycaprolactone beads and autologous myoblasts in a dog model of fecal incontinence.
abstract: OBJECTIVE
  Basic fibroblastic growth factor (bFGF), a member of the heparin-binding growth factor family, regulates muscle differentiation. We investigated whether coadministration of autologous myoblasts and bFGF-loaded polycaprolactone beads could improve sphincter recovery in a dog model of fecal incontinence (FI).
  
  
  METHODS
  FI was induced by resecting 25% of the posterior anal sphincter in ten mongrel dogs. One month later, the dogs were randomized to receive either PKH-26-labeled autologous myoblasts alone (M group, five dogs) or autologous myoblasts and bFGF-loaded polycaprolactone beads (MBG group, five dogs). The outcomes included anal manometry, compound muscle action potentials (CMAPs) of the pudendal nerve, and histology.
  
  
  RESULTS
  The increase in anal contractile pressure over 3 months was significantly greater in the MBG group (from 4.85 to 6.83 mmHg) than that in the M group (from 4.94 to 4.25 mmHg), with a coefficient for the difference in recovery rate of 2.672 (95% confidence interval [CI] 0.962 to 4.373, p = 0.002). The change in the CMAP amplitude was also significantly greater in the MBG group (from 0.59 to 1.56 mV) than that in the M group (from 0.81 to 0.67 mV) (coefficient 1.114, 95% CI 0.43 to 1.80, p = 0.001). Labeled cells were detected in 2/5 (40%) and 5/5 (100%) dogs in the M and MBG groups, respectively.
  
  
  CONCLUSIONS
  Coadministration of bFGF-loaded PCL beads and autologous myoblasts improved the recovery of sphincter function in a dog model of FI and had better outcomes than cell-based therapy alone.
authors.affiliation: Department of Surgery, Seoul National University College of Medicine, Seoul National University Bundang Hospital, 300 Gumi-dong Bundang-gu, Seongnam-si, Gyeonggi-do, 463-707, Republic of Korea.
authors.forename: Heung-Kwon
authors.initials: HK
authors.lastname: Oh
authors.forename: Hye Seung
authors.initials: HS
authors.lastname: Lee
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Lim
authors.forename: Soyeon
authors.initials: S
authors.lastname: Ahn
authors.forename: Sung-Bum
authors.initials: SB
authors.lastname: Kang
mesh_terms: D001003:Anal Canal / Q000503:physiopathology; D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D004337:Drug Carriers; D005242:Fecal Incontinence / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D048091:Guided Tissue Regeneration / Q000379:methods*; D009119:Muscle Contraction; D032446:Myoblasts / Q000637:transplantation*; D011091:Polyesters; D011312:Pressure; D011897:Random Allocation; D014182:Transplantation, Autologous
chemical_list: D004337:Drug Carriers; D011091:Polyesters; D016222:Fibroblast Growth Factor 2; C016240:polycaprolactone