title: MicroRNA-22-3p is down-regulated in the plasma of Han Chinese patients with premature ovarian failure.
abstract: OBJECTIVE
  To determine whether plasma microRNAs (miRNAs) are differentially expressed between women with and without premature ovarian failure (POF), and to uncover the association of miRNAs with risk of POF.
  
  
  METHODS
  Microarray with real-time polymerase chain reaction validation.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  A total of 140 individuals with premature ovarian failure (POF) and 140 age- and body mass index-matched control subjects of Han Chinese ancestry.
  
  
  METHODS
  None.
  
  
  METHODS
  Relative miRNA expression levels in plasma of POF and control group.
  
  
  RESULTS
  Fifty-one differentially expressed miRNAs were identified by chip-based discovery stage between ten patients with POF and ten control subjects, among which nine miRNAs (let-7b-5p, let-7c, miR-15b-5p, miR-22-3p, miR-23a-3p, miR-23b-3p, miR-24-3p, miR-151a-5p, and miR-151b) were selected and validated. The relative expression level of miR-22-3p was significantly down-regulated in POF compared with control subjects. MiR-22-3p yielded a receiver operating characteristic (ROC) curve area of 0.668 (95% confidence interval 0.602-0.733) in discriminating POF from controls. In addition, logistic binary regression analysis and linear regression analysis showed the miR-22-3p to be a protective factor for POF (odds ratio 0.766, 95% CI 0.643-0.912) and negatively associated with serum FSH. Furthermore, bioinformatics analysis indicated that the target function of miR-22-3p was involved in apoptosis, endocytosis, and tumorigenesis.
  
  
  CONCLUSIONS
  Mir-22-3p showed a lower expression level in POF and was modestly effective in distinguishing POF from control subjects. The decreased expression of miR-22-3p in plasma of POF may reflect the diminished ovarian reserve and be a consequence of the pathologic process of POF.
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology of the Ministry of Education, and Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Dang
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology of the Ministry of Education, and Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China.
authors.forename: Shidou
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology of the Ministry of Education, and Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Qin
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology of the Ministry of Education, and Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, Renji Hospital Affiliated with Shanghai Jiaotong University, Shanghai, People's Republic of China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Key Laboratory for Reproductive Endocrinology of the Ministry of Education, and Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan, People's Republic of China. Electronic address: chenzijiang@hotmail.com.
authors.forename: Zi-Jiang
authors.initials: ZJ
authors.lastname: Chen
mesh_terms: D000328:Adult; D044466:Asian People; D016022:Case-Control Studies; D015536:Down-Regulation / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000097:blood*; D046228:Microarray Analysis; D065851:Ovarian Reserve / Q000235:genetics; D016649:Primary Ovarian Insufficiency / Q000097:blood* / Q000208:ethnology / Q000235:genetics; D055815:Young Adult
chemical_list: C548375:MIRN22 microRNA, human; D035683:MicroRNAs
keywords: Premature ovarian failure (POF); microRNA; microRNA-22-3p; plasma; primary ovarian insufficiency (POI)