title: Synthesis and immunestimulating activity of lactobacilli-originated polysaccharide-polymeric microparticle conjugates.
abstract: The design and synthesis of biomaterials capable of activating the immune system are of interest in immunology-related fields because of their ability to tune up the immune defenses of the host. Lactobacilli are a major constituent of normal human indigenous flora, and some specific strains are known to activate the immune system of the host as probiotics. In this study, we first fabricated novel biohybrid materials in which lactobacilli (L. casei strain Shirota, LcS)-originated polysaccharide-peptidoglycan complexes (PS-PGs) are conjugated with polymeric microparticles (MPs). PS-PGs conjugated onto polymeric MPs surfaces bound its specific antibody, suggesting that PS-PGs kept their original molecular recognition ability. The PS-PGs-based hybrid MPs with an appropriate density of conjugated PS-PGs effectively induced high levels of IL-12 production from macrophages without cytotoxicity. These results suggest that LcS-originated PS-PGs could be available bio-originated materials for developing novel biomaterials capable of activating the immune system in a safe manner.
authors.affiliation: Department of Nanobiochemistry, Frontiers of Innovative Research in Science and Technology (FIRST), Konan University , 7-1-20 Minatojima-Minamimachi, Kobe 650-0047, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Nagahama
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kumano
authors.forename: Tsubasa
authors.initials: T
authors.lastname: Nakata
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Tsuji
authors.forename: Kaoru
authors.initials: K
authors.lastname: Moriyama
authors.forename: Kan
authors.initials: K
authors.lastname: Shida
authors.forename: Koji
authors.initials: K
authors.lastname: Nomoto
authors.forename: Katsuyoshi
authors.initials: K
authors.lastname: Chiba
authors.forename: Kazuya
authors.initials: K
authors.lastname: Koumoto
authors.forename: Jun
authors.initials: J
authors.lastname: Matsui
mesh_terms: D000276:Adjuvants, Immunologic / Q000138:chemical synthesis* / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D007778:Lactobacillus / Q000737:chemistry*; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D011108:Polymers / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000276:Adjuvants, Immunologic; D011108:Polymers; D011135:Polysaccharides, Bacterial