title: Possible Chemical Source of Discrepancy between in Vitro and in Vivo Tests in Nanotoxicology Caused by Strong Adsorption of Buffer Components.
abstract: In the course of studies of the interaction of proteins with TiO2 nanoparticles, we have investigated the role of the medium employed in cellular tests, by measuring the variation of ζ-potential vs pH in the range 2-9 and bovine serum albumin adsorption on TiO2 P25 in the presence of either HEPES or PBS as buffers, both mimicking the physiological pH, but with different chemical nature. The two buffers yield remarkably dissimilar surface charges and protein uptake, i.e., they impart different surface characteristics to the particles which could affect the contact with cells or tissues. This may account for dissimilar toxicological outcomes among in vitro tests and particularly between in vitro vs in vivo tests, considering the high amount of phosphate ions present in body fluids.
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Arianna
authors.initials: A
authors.lastname: Marucco
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Catalano
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Ivana
authors.initials: I
authors.lastname: Fenoglio
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Turci
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Gianmario
authors.initials: G
authors.lastname: Martra
authors.affiliation: Department of Chemistry, Interdepartmental Centre "G. Scansetti" for Studies on Asbestos and Other Toxic Particulates, and Interdepartmental Center for Nanostructured Interfaces and Surfaces (NIS), University of Torino , Via P. Giuria 7, 10125 Torino, Italy.
authors.forename: Bice
authors.initials: B
authors.lastname: Fubini
mesh_terms: D000327:Adsorption; D002021:Buffers; D018929:Cell Culture Techniques; D006531:HEPES / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D010710:Phosphates / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000633:toxicity; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D002021:Buffers; D010710:Phosphates; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D014025:Titanium; D006531:HEPES