title: A microfluidic device to study cancer metastasis under chronic and intermittent hypoxia.
abstract: Metastatic cancer cells must traverse a microenvironment ranging from extremely hypoxic, within the tumor, to highly oxygenated, within the host's vasculature. Tumor hypoxia can be further characterized by regions of both chronic and intermittent hypoxia. We present the design and characterization of a microfluidic device that can simultaneously mimic the oxygenation conditions observed within the tumor and model the cell migration and intravasation processes. This device can generate spatial oxygen gradients of chronic hypoxia and produce dynamically changing hypoxic microenvironments in long-term culture of cancer cells.
authors.affiliation: UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University , 4206D Engineering Building III, 911 Oval Drive, Raleigh, North Carolina 27695-7115, USA.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Acosta
authors.affiliation: UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University , 4206D Engineering Building III, 911 Oval Drive, Raleigh, North Carolina 27695-7115, USA.
authors.forename: Xiao
authors.identifier: 0000-0001-8307-2170
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology , No. 43, Sec. 4, Keelung Road, Da'an District, Taipei City 106, Taiwan.
authors.forename: Pin-Kang
authors.initials: PK
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, Beckman Laser Institute, University of California Irvine , 1002 Health Services Road, Irvine, California 92617, USA.
authors.forename: Kyle B
authors.identifier: 0000-0001-5735-8423
authors.initials: KB
authors.lastname: Cutler
authors.affiliation: UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University , 4206D Engineering Building III, 911 Oval Drive, Raleigh, North Carolina 27695-7115, USA.
authors.forename: Christine S
authors.initials: CS
authors.lastname: Grant
authors.affiliation: UNC/NCSU Joint Department of Biomedical Engineering, North Carolina State University , 4206D Engineering Building III, 911 Oval Drive, Raleigh, North Carolina 27695-7115, USA.
authors.forename: Glenn M
authors.initials: GM
authors.lastname: Walker
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology , No. 43, Sec. 4, Keelung Road, Da'an District, Taipei City 106, Taiwan.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Gamcsik