title: Expression of fucosyltransferase 8 is associated with an unfavorable clinical outcome in non-small cell lung cancers.
abstract: Objecitive: Fucosyltransferase 8 (FUT8), the only enzyme responsible for the core α1,6-fucosylation of asparagine-linked oligosaccharides of glycoproteins, is a vital enzyme in cancer development and progression. We examined FUT8 expression in non-small cell lung cancers (NSCLCs) to analyze its clinical significance. We also examined the expression of guanosine diphosphate-mannose-4,6-dehydratase (GMD), which is imperative for the synthesis of fucosylated oligosaccharides.
  
  
  METHODS
  Using immunohistochemistry, we evaluated the expression of FUT8 and GMD in relation to patient survival and prognosis in potentially curatively resected NSCLCs.
  
  
  RESULTS
  High expression of FUT8 was found in 67 of 129 NSCLCs (51.9%) and was significantly found in non-squamous cell carcinomas (p = 0.008). High expression of FUT8 was associated with poor survival (p = 0.03) and was also a significant and independent unfavorable prognostic factor in patients with potentially curatively resected NSCLCs (p = 0.047). High expression of GMD was significantly associated with high FUT8 expression (p = 0.04).
  
  
  CONCLUSIONS
  High expression of FUT8 is associated with an unfavorable clinical outcome in patients with potentially curatively resected NSCLCs, suggesting that FUT8 can be a prognostic factor. The analysis of FUT8 expression and its core fucosylated products may provide new insights for the therapeutic targets of NSCLCs.
authors.affiliation: Department of Medical Oncology, Hokkaido University Graduate School of Medicine, Sapporo, Japan.
authors.forename: Rio
authors.initials: R
authors.lastname: Honma
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kinoshita
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyoshi
authors.forename: Utano
authors.initials: U
authors.lastname: Tomaru
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Matsuno
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Shimizu
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Kichizo
authors.initials: K
authors.lastname: Kaga
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Taniguchi
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Dosaka-Akita
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology / Q000401:mortality; D000077192:Adenocarcinoma of Lung; D000368:Aged; D015153:Blotting, Western; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006801:Humans; D006836:Hydro-Lyases / Q000378:metabolism; D007150:Immunohistochemistry; D007564:Japan / Q000453:epidemiology; D053208:Kaplan-Meier Estimate; D008168:Lung / Q000201:enzymology; D008175:Lung Neoplasms / Q000201:enzymology* / Q000401:mortality* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D018570:Risk Assessment; D012307:Risk Factors
chemical_list: D006155:Guanosine Diphosphate Mannose; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase; D006836:Hydro-Lyases