title: Skin reaction and regeneration after single sodium lauryl sulfate exposure stratified by filaggrin genotype and atopic dermatitis phenotype.
abstract: BACKGROUND
  Filaggrin is key for the integrity of the stratum corneum. Mutations in the filaggrin gene (FLGnull) play a prominent role in atopic dermatitis (AD) pathogenesis. People with AD have increased susceptibility to irritants. However, little is known about the effect of filaggrin genotype and AD phenotype on irritant response and skin regeneration.
  
  
  OBJECTIVE
  To investigate the role of FLGnull and AD groups for skin reaction and recovery after sodium lauryl sulfate (SLS) irritation.
  
  
  METHODS
  This is a case-control study comprising 67 subjects, including healthy controls and patients with and without FLGnull and AD. Reactivity to different doses of SLS at 24, 48, 72 and 145 h after SLS application was measured by transepidermal water loss (TEWL) and laser Doppler flowmetry (LDF). Reactivity was assessed univariately and by pattern analysis.
  
  
  RESULTS
  All patient groups showed a higher degree of skin-barrier disruption and inflammation than did controls in response to SLS. Assessing reactivity by the delta value of the area under the curve for both TEWL and LDF showed significant differences between healthy controls and those with the AD phenotype, irrespective of filaggrin mutation. The poorest regeneration was among those with the AD phenotype. The two AD phenotype groups were separated by multivariate technique, due to earlier inflammatory reactivity among subjects with FLGnullplus AD compared with the AD phenotype alone.
  
  
  CONCLUSIONS
  Both skin reaction and regeneration were significantly different between the patient population and the healthy controls. Additionally, response severity and regeneration depended more on AD phenotype than on filaggrin genotype, whereas the response was more rapid among the FLGnullplus AD individuals.
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårdsvej 28, 2900, Hellerup, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Bandier
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårdsvej 28, 2900, Hellerup, Denmark.
authors.forename: B C
authors.initials: BC
authors.lastname: Carlsen
authors.affiliation: Faculty of Science, University of Copenhagen, Frederiksberg, Denmark.
authors.forename: M A
authors.initials: MA
authors.lastname: Rasmussen
authors.affiliation: Department of Nuclear Medicine, Aalborg University Hospital, Aalborg, Denmark.|Department of Clinical Medicine, Aalborg University Hospital, Aalborg, Denmark.
authors.forename: L J
authors.initials: LJ
authors.lastname: Petersen
authors.affiliation: National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårdsvej 28, 2900, Hellerup, Denmark.
authors.forename: J D
authors.initials: JD
authors.lastname: Johansen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000503:physiopathology; D017453:Dermatitis, Irritant / Q000235:genetics / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D005260:Female; D000091344:Filaggrin Proteins; D005838:Genotype; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007509:Irritants / Q000008:administration & dosage / Q000009:adverse effects; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D011897:Random Allocation; D012038:Regeneration / Q000235:genetics*; D012879:Skin Physiological Phenomena / Q000235:genetics*; D012967:Sodium Dodecyl Sulfate / Q000008:administration & dosage / Q000009:adverse effects*; D014870:Water Loss, Insensible / Q000235:genetics; D055815:Young Adult
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D007509:Irritants; D012967:Sodium Dodecyl Sulfate