title: Extracellular vesicle-mediated export of fungal RNA.
abstract: Extracellular vesicles (EVs) play an important role in the biology of various organisms, including fungi, in which they are required for the trafficking of molecules across the cell wall. Fungal EVs contain a complex combination of macromolecules, including proteins, lipids and glycans. In this work, we aimed to describe and characterize RNA in EV preparations from the human pathogens Cryptococcus neoformans, Paracoccidiodes brasiliensis and Candida albicans, and from the model yeast Saccharomyces cerevisiae. The EV RNA content consisted mostly of molecules less than 250 nt long and the reads obtained aligned with intergenic and intronic regions or specific positions within the mRNA. We identified 114 ncRNAs, among them, six small nucleolar (snoRNA), two small nuclear (snRNA), two ribosomal (rRNA) and one transfer (tRNA) common to all the species considered, together with 20 sequences with features consistent with miRNAs. We also observed some copurified mRNAs, as suggested by reads covering entire transcripts, including those involved in vesicle-mediated transport and metabolic pathways. We characterized for the first time RNA molecules present in EVs produced by fungi. Our results suggest that RNA-containing vesicles may be determinant for various biological processes, including cell communication and pathogenesis.
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia da Escola Paulista de Medicina-UNIFESP, São Paulo, SP, Brazil.
authors.forename: Roberta
authors.initials: R
authors.lastname: Peres da Silva
authors.affiliation: Departamento de Microbiologia, Imunologia e Parasitologia da Escola Paulista de Medicina-UNIFESP, São Paulo, SP, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Puccia
authors.affiliation: 1] Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil [2] Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Marcio L
authors.initials: ML
authors.lastname: Rodrigues
authors.affiliation: Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Débora L
authors.initials: DL
authors.lastname: Oliveira
authors.affiliation: Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
authors.forename: Luna S
authors.initials: LS
authors.lastname: Joffe
authors.affiliation: Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Gabriele V
authors.initials: GV
authors.lastname: César
authors.affiliation: Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Nimrichter
authors.affiliation: Instituto Carlos Chagas, Fundação Oswaldo Cruz, Fiocruz-PR, Curitiba, PR, Brazil.
authors.forename: Samuel
authors.initials: S
authors.lastname: Goldenberg
authors.affiliation: Instituto Carlos Chagas, Fundação Oswaldo Cruz, Fiocruz-PR, Curitiba, PR, Brazil.
authors.forename: Lysangela R
authors.initials: LR
authors.lastname: Alves
mesh_terms: D001483:Base Sequence; D005091:Exons / Q000235:genetics; D005110:Extracellular Space / Q000378:metabolism*; D005453:Fluorescence; D005658:Fungi / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D063990:Gene Ontology; D016681:Genome, Fungal; D006801:Humans; D007438:Introns / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics; D034443:RNA Transport; D012331:RNA, Fungal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D022661:RNA, Untranslated / Q000235:genetics; D022142:Secretory Vesicles / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D035683:MicroRNAs; D012331:RNA, Fungal; D012333:RNA, Messenger; D022661:RNA, Untranslated