title: Structure and properties of porous films based on aliphatic copolyamide developed for cellular technologies.
abstract: The effect of concentration and viscosity of the copolyamide (copolymer of ε-caprolactam and hexamethylendiaminadipate) solutions in aqueous/alcoholic solvents on its phase state was studied. The films obtained by the coagulation method were characterized by monodisperse pore distribution with an average pore size of 1.3 μm. The films processed by electrospinning from copolyamide solutions were characterized by a bimodal distribution of macropores with one peak of pore radius at 2.0 μm and second peak of pore radius at 20 μm. The adhesion and proliferation of mesenchymal adhesion stem cells (ASCs) stem cells to copolyamide matrix were studied. With the help of scanning electron microscopy it was shown that both tapes porous films were characterized by good adhesion of mesenchymal ASCs stem cells. It was shown that the porous structure, transport and mechanical properties of these copolyamide films allow their use as two-dimensional matrices for cellular technology.
authors.affiliation: Institute of Macromolecular Compounds Russian Academy of Sciences, 31 Bolshoy prospekt VO, 199004, Saint Petersburg, Russia, dobrov@hq.macro.ru.
authors.forename: I P
authors.initials: IP
authors.lastname: Dobrovolskaya
authors.forename: P V
authors.initials: PV
authors.lastname: Popryadukhin
authors.forename: V E
authors.initials: VE
authors.lastname: Yudin
authors.forename: E M
authors.initials: EM
authors.lastname: Ivan'kova
authors.forename: V Yu
authors.initials: VY
authors.lastname: Elokhovskiy
authors.forename: Z
authors.initials: Z
authors.lastname: Weishauptova
authors.forename: K
authors.initials: K
authors.lastname: Balik
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D006801:Humans; D008027:Light; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry; D009757:Nylons / Q000737:chemistry*; D010539:Permeability; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D012212:Rheology; D012542:Scattering, Radiation; D013234:Stem Cells / Q000166:cytology*; D013314:Stress, Mechanical; D014783:Viscosity; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D009757:Nylons; D011108:Polymers; D014867:Water