title: Reduced expression of microRNA‑206 regulates cell proliferation via cyclinD2 in gliomas.
abstract: MicroRNAs are short single‑stranded non‑coding RNA molecules that function as regulators of tumor progression, including regulation of glioblastoma multiforme, which is a World Health Organization grade Ⅳ glioma. Based on the results of a microRNA microarray, which included 198 patients with glioma from the Chinese Glioma Genome Atlas data set, it was observed that microRNA‑206 (miR‑206) was downregulated in high-grade (grades Ⅲ and Ⅳ) gliomas compared with grade II gliomas. In addition, high expression of miR‑206 was associated with longer overall survival time in glioma patients. The present study aimed to investigate the biological functions of miR‑206 in glioma progression in vitro using the LN229 glioma cell line. Cell proliferation was observed to be inhibited subsequent to transfection with miR‑206. It was suggested that miR‑206 induced cell cycle G1/S phase arrest by suppressing the expression of cyclinD2. The results of the present study concluded that miR‑206 inhibits glioma progression via the regulation of cyclinD2 and that miR‑206 may be a novel biomarker with potential for use as a therapeutic target in gliomas.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Xue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Beijing Neurosurgical Institute, Beijing 100050, P.R. China.
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Tianzhu
authors.initials: T
authors.lastname: Guo
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Qingyang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, P.R. China.
authors.forename: Quangeng
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D056742:Cyclin D2 / Q000737:chemistry / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000737:chemistry / Q000235:genetics*; D060787:Neoplasm Grading; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D056742:Cyclin D2; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger