title: Establishment of indirect enzyme-linked immunosorbent assay for artificial musk.
abstract: Anti-inflammatory component (AIC) known as an important constituent of artificial musk exhibits bioactive effects on many pharmacological models. This study describes an immunochemical assay for the quality control of artificial musk in traditional Chinese medicine using an enzyme-linked immunosorbent assay. A polyclonal antibody against AIC was produced by rabbit. The method, at an effective measuring range of 3.13-200 ng/ml of AIC, 3 ng/ml limit of detection, and no cross-reaction with natural musk, successfully detected artificial musk in many traditional Chinese prescriptions containing artificial musk. The results demonstrated that a novel and reliable assay system for detection of artificial musk was generated.
authors.affiliation: a State Key Laboratory of Bioactive Substance and Function of Natural Medicines , Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences , Beijing 100050 , China.
authors.forename: Fang
authors.initials: F
authors.lastname: Shen
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Bai
authors.forename: Yu
authors.initials: Y
authors.lastname: Meng
authors.forename: Xuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiu-Yuan
authors.initials: XY
authors.lastname: Zhu
authors.forename: Gui-Fang
authors.initials: GF
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis / Q000276:immunology / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005229:Fatty Acids, Monounsaturated / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D008297:Male; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D011817:Rabbits
chemical_list: D000893:Anti-Inflammatory Agents; D005229:Fatty Acids, Monounsaturated; C008563:musk
keywords: ELISA; artificial musk; specific detection