title: LC-MS-based metabolomics study of marine bacterial secondary metabolite and antibiotic production in Salinispora arenicola.
abstract: An LC-MS-based metabolomics approach was used to characterise the variation in secondary metabolite production due to changes in the salt content of the growth media as well as across different growth periods (incubation times). We used metabolomics as a tool to investigate the production of rifamycins (antibiotics) and other secondary metabolites in the obligate marine actinobacterial species Salinispora arenicola, isolated from Great Barrier Reef (GBR) sponges, at two defined salt concentrations and over three different incubation periods. The results indicated that a 14 day incubation period is optimal for the maximum production of rifamycin B, whereas rifamycin S and W achieve their maximum concentration at 29 days. A "chemical profile" link between the days of incubation and the salt concentration of the growth medium was shown to exist and reliably represents a critical point for selection of growth medium and harvest time.
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia. utpal.bose@uqconnect.edu.au.
authors.forename: Utpal
authors.initials: U
authors.lastname: Bose
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia. a.hewavitharana@pharmacy.uq.edu.au.
authors.forename: Amitha K
authors.initials: AK
authors.lastname: Hewavitharana
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia. Yi.ng@uqconnect.edu.au.
authors.forename: Yi Kai
authors.initials: YK
authors.lastname: Ng
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia. n.shaw@pharmacy.uq.edu.au.
authors.forename: Paul Nicholas
authors.initials: PN
authors.lastname: Shaw
authors.affiliation: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia. j.fuerst@uq.edu.au.
authors.forename: John A
authors.initials: JA
authors.lastname: Fuerst
authors.affiliation: School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia. m.hodson1@uq.edu.au.
authors.forename: Mark P
authors.initials: MP
authors.lastname: Hodson
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D013058:Mass Spectrometry; D055432:Metabolomics; D008848:Micromonosporaceae / Q000737:chemistry* / Q000378:metabolism; D011161:Porifera / Q000382:microbiology; D012294:Rifamycins / Q000302:isolation & purification / Q000378:metabolism; D012623:Seawater / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D012294:Rifamycins