title: Prevention of glucocorticoid induced bone changes with beta-ecdysone.
abstract: Beta-ecdysone (βEcd) is a phytoecdysteroid found in the dry roots and seeds of the asteraceae and achyranthes plants, and is reported to increase osteogenesis in vitro. Since glucocorticoid (GC) excess is associated with a decrease in bone formation, the purpose of this study was to determine if treatment with βEcd could prevent GC-induced osteoporosis. Two-month-old male Swiss-Webster mice (n=8-10/group) were randomized to either placebo or slow release prednisolone pellets (3.3mg/kg/day) and treated with vehicle control or βEcd (0.5mg/kg/day) for 21days. GC treatment inhibited age-dependent trabecular gain and cortical bone expansion and this was accompanied by a 30-50% lower bone formation rate (BFR) at both the endosteal and periosteal surfaces. Mice treated with only βEcd significantly increased bone formation on the endosteal and periosteal bone surfaces, and increased cortical bone mass were their controls to compare to GC alone. Concurrent treatment of βEcd and GC completely prevented the GC-induced reduction in BFR, trabecular bone volume and partially prevented cortical bone loss. In vitro studies determined that βEcd prevented the GC increase in autophagy of the bone marrow stromal cells as well as in whole bone. In summary, βEcd prevented GC induced changes in bone formation, bone cell viability and bone mass. Additional studies are warranted of βEcd for the treatment of GC induced bone loss.
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA; Department of Science and Technology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Dai
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Yu-An Evan
authors.initials: YA
authors.lastname: Lay
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Science and Technology, Longhua Hospital Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
authors.forename: Guoqin
authors.initials: G
authors.lastname: Jin
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Kot
authors.affiliation: Department of Materials Science and Engineering, University of California at Berkeley, Berkeley, CA 94720, USA.
authors.forename: Robert O
authors.initials: RO
authors.lastname: Ritchie
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA.
authors.forename: Nancy E
authors.initials: NE
authors.lastname: Lane
authors.affiliation: Center for Musculoskeletal Health, Internal Medicine, University of California at Davis Medical Center, Sacramento, CA 95817, USA. Electronic address: wei.yao@ucdmc.ucdavis.edu.
authors.forename: Wei
authors.initials: W
authors.lastname: Yao
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D001696:Biomechanical Phenomena / Q000187:drug effects; D001835:Body Weight / Q000528:radiation effects; D016723:Bone Remodeling / Q000187:drug effects; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D002454:Cell Differentiation / Q000187:drug effects; D004441:Ecdysterone / Q000494:pharmacology*; D005938:Glucocorticoids / Q000009:adverse effects*; D006728:Hormones / Q000097:blood; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D055114:X-Ray Microtomography
chemical_list: D005938:Glucocorticoids; D006728:Hormones; D004441:Ecdysterone
keywords: Autophagy; Beta-ecdysone (βEcd); Bone formation; Glucocorticoid