title: Stable emulsions prepared by self-assembly of hyaluronic acid and chitosan for papain loading.
abstract: A simple, green and effective process is developed to fabricate hyaluronic acid (HA)/chitosan (CS) complex colloidal particles through electrostatic interactions. The obtained complexes can be used as biocompatible emulsifiers and novel potential carriers for papain loading. An HA/CS mass ratio of 2 is the optimal condition leading to the smallest Dh (420.9 nm). The complexes with eight different mass ratios are used to stabilize white oil/water emulsions. The structure of the complexes at the oil-water interface varies in response to the mass ratio and can be classified into two typical structures, similar to typical polymeric surfactants and solid particulate emulsifiers. Furthermore, papain is introduced into the complex systems. Formation of the papain/HA/CS complexes in a compact form can protect the enzyme. Here, a novel strategy is introduced to fabricate a biocompatible emulsion from the HA/CS complexes and demonstrate that the stable complex is a suitable enzyme delivery system.
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu, 214122, People's Republic of China.
authors.forename: Donghua
authors.initials: D
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jianhua
authors.initials: J
authors.lastname: Sun
authors.forename: Qiong
authors.initials: Q
authors.lastname: Hu
authors.forename: Xiaoya
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D048271:Chitosan / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers; D004655:Emulsions / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D010206:Papain / Q000008:administration & dosage* / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D004655:Emulsions; D006820:Hyaluronic Acid; D048271:Chitosan; D010206:Papain
keywords: chitosan; complexes; emulsions; enzyme carriers; hyaluronic acid