title: Effect of Zhuyun recipe on endometrial pinopode expression in mice with embryonic implantation dysfunction and ovulation stimulation.
abstract: Zhuyun recipe (ZYR) is a traditional Chinese medicine that has been widely used as an infertility treatment for a number of years. Although the therapeutic effects are desirable and satisfactory, the therapeutic mechanism of ZYR remains poorly understood. In the present study, pinopodes were investigated as an important morphological marker of endometrial receptivity, in order to further investigate the therapeutic mechanism of ZYR. The expression of pinopodes during the implantation window was observed using scanning electron microscopy in mice with induced ovarian stimulation (OS) and embryo implantation dysfunction (EID). A marked decrease in the number of fully developed pinopodes was observed on the endometrial surface in the OS and EID model mice, which was in accordance with the decreased pregnancy rate and number of embryonic implantation sites when compared with the control. Following treatment with ZYR, the spatial and temporal expression of pinopodes in the OS and EID mice was found to be similar to the control mice. In conclusion, ZYR was demonstrated to improve endometrial receptivity in OS and EID mice through significant improvements in the spatial and temporal expression of pinopodes.
authors.affiliation: Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Yu
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Yan
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Tailang
authors.initials: T
authors.lastname: Yin
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Reproductive Medical Center, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
keywords: embryo implantation dysfunction; endometrial receptivity; mouse; ovarian stimulation; pinopodes; traditional Chinese medicine