title: Determination of RNA chaperone activity using an Escherichia coli mutant.
abstract: Bacterial cold shock proteins (CSPs) function as RNA chaperones that destabilize RNA secondary structures. A quadruple mutant of Escherichia coli (cspA, cspB, cspG, and cspE) displays a cold-sensitive growth phenotype. Plant cold shock domain (CSD) proteins have been shown to complement the cold-sensitive phenotype of the E. coli mutant and to share a function with E. coli CSPs as RNA chaperones. This methodology, which is detailed here, can be utilized to reveal or probe the RNA chaperone activity of heterologous proteins.
authors.affiliation: Crop Breeding Research Division, Hokkaido Agricultural Research Center, National Agriculture and Food Research Organization, Hitsujigaoka, Sapporo, 062-8555, Japan.
authors.forename: Myung-Hee
authors.initials: MH
authors.lastname: Kim
authors.forename: Ryozo
authors.initials: R
authors.lastname: Imai
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D057773:Cold Shock Proteins and Peptides / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism*; D016601:RNA-Binding Proteins
chemical_list: D002352:Carrier Proteins; D057773:Cold Shock Proteins and Peptides; C498192:CspA protein, E coli; C491179:CspB protein, E coli; C088097:CspE protein, E coli; C118134:CspG protein, E coli; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D012329:RNA, Bacterial; D016601:RNA-Binding Proteins