title: An injured tissue affects the opposite intact peritoneum during postoperative adhesion formation.
abstract: The pathophysiology of adhesion formation needs to be clarified to reduce the adhesion-related morbidity. The epithelial characteristics of the peritoneum suggest a protective role against adhesion formation, yet how the peritoneum is involved in adhesion formation is not well characterized. We microscopically observed an experimental model of adhesion formation to investigate the effects of an injured tissue on the opposite intact peritoneum. Adhesions were induced between injured and intact hepatic lobes, and the intact peritoneum opposite to the injured tissue was examined for 8 days. The opposite intact peritoneum was denuded of mesothelial cells for 6 hours, and the remnant mesothelial cells changed morphologically for 24 hours. The detachment of mesothelial cells allowed fibrin to attach to the basement membrane of the opposite peritoneum, connecting the two lobes. Moreover, macrophages and myofibroblasts accumulated between the two lobes, and angiogenesis occurred from the opposite intact lobe to the injured lobe. These observations indicate that an injured tissue deprives the opposite intact peritoneum of its epithelial structure and causes fibrous adhesions to the opposite intact tissue. This study implies a possible role of mesothelial cells for barrier function against adhesion formation, that is, keeping mesothelial cells intact might lead to its prophylaxis.
authors.affiliation: Division of Gastroenterologic and General Surgery, Department of Surgery, Asahikawa Medical University, Hokkaido, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: 1] Advanced Surgery Center, Sapporo Higashi Tokushukai Hospital, Hokkaido, Japan [2] Faculty of Pharmaceutical Sciences, Hokkaido University, Hokkaido, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Kono
authors.affiliation: Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, Hokkaido, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Bochimoto
authors.affiliation: Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, Hokkaido, Japan.
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Hira
authors.affiliation: Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, Hokkaido, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: Division of Gastroenterologic and General Surgery, Department of Surgery, Asahikawa Medical University, Hokkaido, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D004549:Elastin / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005337:Fibrin / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D010537:Peritoneum / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000267:Tissue Adhesions
chemical_list: D005337:Fibrin; D004549:Elastin