title: A simple and sensitive LC-MS/MS method for the determination of sotalol in rat plasma.
abstract: A sensitive, rapid and robust HPLC method with tandem mass spectrometry (HPLC/MS/MS) detection has been developed and validated for the quantification of sotalol in rat plasma. Plasma samples were precipitated with acetonitrile before analysis. The chromatographic separation was performed on an Atlantis hydrophilic interaction liquid chromatography Silica column (50 × 2.1 mm, 3 µm) with a gradient mobile phase of 10 mm NH4 COOH (containing 0.2% of formic acid) as buffer A and acetonitrile as mobile phase B. Sotalol (m/z 273.2 → 255.1) and atenolol (the internal standard, IS, m/z 267.2 → 190.1) were monitored under positive ionization mode with 5500 QTRAP. Retention time of sotalol and the IS were 2.69 and 3.43 min, respectively. The linear range was 5-500 nm based on the analysis of 0.1 mL of plasma. The intrabatch precision ranged from 1.2 to 6.1%, and the inter-batch precision was from 3.3 to 6.5%. The coefficient of variation of IS-normalized matrix factor was 7.6%. Experiments for stability were performed and the analyte was sufficiently stable. A run time of 6 min for each injection made it possible to analyze a high throughput of plasma samples. The assay was successfully applied to the determination of sotalol in rat plasma after a micro-dose oral administration.
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.|Department of Pharmacy, Jiangmen Central Hospital Affiliated Jiangmen Hospital of Sun Yat-Sen, Jiangmen City, Guangdong Province, 529000, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.|Department of Pharmacy, Jiangmen Central Hospital Affiliated Jiangmen Hospital of Sun Yat-Sen, Jiangmen City, Guangdong Province, 529000, China.
authors.forename: Siyuan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Suodi
authors.initials: S
authors.lastname: Zhai
authors.affiliation: Department of Pharmacy, Jiangmen Central Hospital Affiliated Jiangmen Hospital of Sun Yat-Sen, Jiangmen City, Guangdong Province, 529000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Peking University Third Hospital, Beijing, 100191, China.
authors.forename: Xianhua
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000319:Adrenergic beta-Antagonists / Q000097:blood*; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013015:Sotalol / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*
chemical_list: D000319:Adrenergic beta-Antagonists; D000889:Anti-Arrhythmia Agents; D013015:Sotalol
keywords: LC-MS/MS; rat plasma; sotalol; validation