title: X-DNA origami-networked core-supported lipid stratum.
abstract: DNA hydrogels are promising materials for various fields of research, such as in vitro protein production, drug carrier systems, and cell transplantation. For effective application and further utilization of DNA hydrogels, highly effective methods of nano- and microscale DNA hydrogel fabrication are needed. In this respect, the fundamental advantages of a core-shell structure can provide a simple remedy. An isolated reaction chamber and massive production platform can be provided by a core-shell structure, and lipids are one of the best shell precursor candidates because of their intrinsic biocompatibility and potential for easy modification. Here, we demonstrate a novel core-shell nanostructure made of gene-knitted X-shaped DNA (X-DNA) origami-networked gel core-supported lipid strata. It was simply organized by cross-linking DNA molecules via T4 enzymatic ligation and enclosing them in lipid strata. As a condensed core structure, the DNA gel shows Brownian behavior in a confined area. It has been speculated that they could, in the future, be utilized for in vitro protein synthesis, gene-integration transporters, and even new molecular bottom-up biological machineries.
authors.affiliation: School of Chemical Engineering and ‡SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University , Suwon 440-746, South Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Park
authors.forename: Min Su
authors.initials: MS
authors.lastname: Jang
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Song
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Seung-Woo
authors.initials: SW
authors.lastname: Cho
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Jeong Ho
authors.initials: JH
authors.lastname: Cho
authors.forename: Sunghwan
authors.initials: S
authors.lastname: Jung
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry / Q000201:enzymology; D052160:Benzothiazoles; D002784:Cholesterol / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000138:chemical synthesis / Q000737:chemistry*; D003959:Diamines; D005456:Fluorescent Dyes; D020100:Hydrogels / Q000737:chemistry; D008025:Ligases / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation; D009930:Organic Chemicals; D010713:Phosphatidylcholines / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010715:Phosphatidylglycerols / Q000737:chemistry*; D011804:Quinolines; D014764:Viral Proteins / Q000737:chemistry; D014966:Xanthenes
chemical_list: D052160:Benzothiazoles; D004277:DNA, Single-Stranded; D003959:Diamines; D005456:Fluorescent Dyes; D020100:Hydrogels; D009930:Organic Chemicals; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D011804:Quinolines; D014764:Viral Proteins; D014966:Xanthenes; C098022:SYBR Green I; C007510:1,2-dipalmitoyl-3-phosphatidylethanolamine; C060037:1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; C034657:Texas red; D002784:Cholesterol; D008025:Ligases; C028694:1-palmitoyl-2-oleoylphosphatidylcholine