title: The cluster [Re6Se8I6]3- induces low hemolysis of human erythrocytes in vitro: protective effect of albumin.
abstract: The cluster Re6Se8I63- has been shown to induce preferential cell death of a hepatic carcinoma cell line, thus becoming a promising anti-cancer drug. Whether this cluster induces acute hemolysis or if it interacts with albumin remains unclear. The effect of acute exposure of human red blood cells to different concentrations of the cluster with and without albumin is described. Red blood cells from healthy donors were isolated, diluted at 1% hematocrit and exposed to the cluster (25-150 µM) at 37 °C, under agitation. Hemolysis and morphology were analyzed at 1 and 24 h. The potential protection of 0.1% albumin was also evaluated. Exposition to therapeutic doses of the cluster did not induce acute hemolysis. Similar results were observed following 24 h of exposition, and albumin slightly reduced hemolysis levels. Furthermore, the cluster induced alteration in the morphology of red blood cells, and this was prevented by albumin. Together, these results indicate that the cluster Re6Se8I63- is not a hemolytic component and induces moderate morphological alterations of red blood cells at high doses, which are prevented by co-incubation with albumin. In conclusion, the cluster Re6Se8I63- could be intravenously administered in animals at therapeutic doses for in vivo studies.
authors.affiliation: Universidad Bernardo O' Higgins, Departamento de Ciencias Químicas y Biológicas, General Gana 1780, Santiago 8370854, Chile. eseba21@gmail.com.
authors.forename: Edgardo
authors.initials: E
authors.lastname: Rojas-Mancilla
authors.affiliation: Universidad Bernardo O'Higgins, Escuela de Tecnología Médica, General Gana 1780, Santiago 8370854, Chile. alexis.oyarce@hotmail.com.
authors.forename: Alexis
authors.initials: A
authors.lastname: Oyarce
authors.affiliation: Universidad Bernardo O'Higgins, Escuela de Tecnología Médica, General Gana 1780, Santiago 8370854, Chile. viviana.verdugo@live.com.
authors.forename: Viviana
authors.initials: V
authors.lastname: Verdugo
authors.affiliation: Department of Chemistry, the University of Arizona, Tucson, AZ 85721, USA. zhiping@u.arizona.edu.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Universidad Bernardo O' Higgins, Laboratorio de Bionanotecnología, General Gana 1780, Santiago 8370854, Chile. rodrigoramireztagle@gmail.com.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Ramírez-Tagle
mesh_terms: D000970:Antineoplastic Agents / Q000009:adverse effects* / Q000737:chemistry; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D012211:Rhenium / Q000009:adverse effects* / Q000737:chemistry; D018036:Selenium Compounds / Q000009:adverse effects* / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D018036:Selenium Compounds; D012211:Rhenium