title: Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach.
abstract: Rape seeds primed with -1.2 MPa polyethylene glycol 6000 showed improved germination performance. To better understand the beneficial effect of osmopriming on seed germination, a global expression profiling method was used to compare, for the first time, transcriptomic and proteomic data for osmoprimed seeds at the crucial phases of priming procedure (soaking, drying), whole priming process and subsequent germination. Brassica napus was used here as a model to dissect the process of osmopriming into its essential components. A total number of 952 genes and 75 proteins were affected during the main phases of priming and post-priming germination. Transcription was not coordinately associated with translation resulting in a limited correspondence between mRNAs level and protein abundance. Soaking, drying and final germination of primed seeds triggered distinct specific pathways since only a minority of genes and proteins were involved in all phases of osmopriming while a vast majority was involved in only one single phase. A particular attention was paid to genes and proteins involved in the transcription, translation, reserve mobilization, water uptake, cell cycle and oxidative stress processes.
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University in Poznan, ul. Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Kubala
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University in Poznan, ul. Umultowska 89, 61-614 Poznan, Poland. Electronic address: garnczar@amu.edu.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Garnczarska
authors.affiliation: Department of Plant Physiology, Faculty of Biology, Adam Mickiewicz University in Poznan, ul. Umultowska 89, 61-614 Poznan, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Wojtyla
authors.affiliation: Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 45, boîte L7.07.02, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: André
authors.initials: A
authors.lastname: Clippe
authors.affiliation: Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 34, 60-479 Poznan, Poland.
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Kosmala
authors.affiliation: Laboratory of Molecular and Systems Biology, Institute of Bioorganic Chemistry, Polish Academy of Science, ul. Noskowskiego 12/14, 61-704 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Żmieńko
authors.affiliation: Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute-Agronomy (ELI-A), Université catholique de Louvain, Croix du Sud 45, boîte L7.07.13, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Stanley
authors.initials: S
authors.lastname: Lutts
authors.affiliation: Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute-Agronomy (ELI-A), Université catholique de Louvain, Croix du Sud 45, boîte L7.07.13, B-1348 Louvain-la-Neuve, Belgium.
authors.forename: Muriel
authors.initials: M
authors.lastname: Quinet
mesh_terms: D029741:Brassica rapa / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018525:Germination / Q000235:genetics / Q000502:physiology*; D040901:Proteomics; D059467:Transcriptome
keywords: Brassica napus; Germination; Osmopriming; Polyethylene glycol; Proteome; Transcriptome