title: Expression and clinical role of TCTP in epithelial ovarian cancer.
abstract: The aim of this study is to investigate the potential role and prognostic significance of translationally controlled tumor protein (TCTP) in human epithelial ovarian cancer (EOC). Western blot was used to evaluate the expression of TCTP in eight fresh EOC tissues. Immunohistochemistry was performed on formalin-fixed paraffin-embedded sections of 119 cases of ovarian cancers. Kaplan-Meier method indicated the relation between TCTP and EOC patients' overall survival rate. Starvation and re-feeding was used to assess cell cycle. Knocking down of TCTP and CCK8 assay showed the role of TCTP in HO8910 cell cycle. We found that TCTP was overexpressed in carcinoma tissues compared with normal tissues. Immunohistochemistry revealed that TCTP expression was significantly associated with clinicopathologic variables. Kaplan-Meier analysis revealed that high TCTP expression was significantly related to poor prognosis of the patients. Starvation and re-feeding suggested TCTP played a critical role in HO8910 cell proliferation. Interference of TCTP and CCK8 assay showed that the TCTP-siRNA treated HO8910 cells grew more slowly than the control group. CCK-8 assays and terminal-deoxynucleoitidyl transferase mediated nick end labeling assays were also performed to demonstrate the cisplatin could inhibit the survival of HO8910 cells and promote their apoptosis. All the experiments we have done showed that TCTP could promote the progression of EOC and reduce the sensitiveness of HO8910 cells to cisplatin.
authors.affiliation: Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Nantong University, Nantong, 226001, Jiangsu Province, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Minhui
authors.initials: M
authors.lastname: Hua
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Xi
authors.forename: Rong
authors.initials: R
authors.lastname: Liu
authors.forename: Shuyun
authors.initials: S
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhong
authors.forename: Meilan
authors.initials: M
authors.lastname: Tang
authors.forename: Shumin
authors.initials: S
authors.lastname: Lu
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Min
authors.forename: Chunhui
authors.initials: C
authors.lastname: Tang
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000231:Adenocarcinoma, Papillary / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D005260:Female; D015870:Gene Expression; D006801:Humans; D053208:Kaplan-Meier Estimate; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D016016:Proportional Hazards Models; D000090324:Tumor Protein, Translationally-Controlled 1
chemical_list: D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C000715647:TPT1 protein, human; D000090324:Tumor Protein, Translationally-Controlled 1; D002945:Cisplatin