title: Karyotype characterization and nuclear DNA content measurement in Bromeliaceae: state of the art and future perspectives.
abstract: In Bromeliaceae, cytogenetic and flow cytometry analyses have been performed to clarify systematic and evolutionary aspects. Karyotyping approaches have shown the relatively high chromosome number, similar morphology and small size of the chromosomes. These facts have prevented a correct chromosome counting and characterization. Authors have established a basic chromosome number of x = 25 for Bromeliaceae. Recently, one karyomorphological analysis revealed that x = 25 is no longer the basic chromosome number, whose genome may have a polyploid origin. Besides cytogenetic characterization, the 2C DNA content of bromeliads has been measured. Nuclear DNA content has varied from 2C = 0.60 to 2C = 3.34 picograms. Thus, in relation to most angiosperms, the 2C DNA content of Bromeliaceae species as well as their chromosome size can be considered relatively small. In spite of some advances, cytogenetic and flow cytometry data are extremely scarce in this group. In this context, this review reports the state of the art in karyotype characterization and nuclear DNA content measurement in Bromeliaceae, emphasizing the main problems and suggesting prospective solutions and ideas for future research.
authors.affiliation: Laboratório de Citogenética, Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, ES, Brasil.
authors.forename: Andrei C P
authors.initials: AC
authors.lastname: Nunes
authors.affiliation: Laboratório de Citogenética, Departamento de Biologia, Centro de Ciências Agrárias, Universidade Federal do Espírito Santo, Alegre, ES, Brasil.
authors.forename: Wellington R
authors.initials: WR
authors.lastname: Clarindo
mesh_terms: D031229:Bromeliaceae / Q000145:classification / Q000235:genetics*; D032461:Chromosomes, Plant; D018744:DNA, Plant / Q000235:genetics; D005434:Flow Cytometry; D007621:Karyotyping
chemical_list: D018744:DNA, Plant