title: Reticulon protein-1C is a key component of MAMs.
abstract: The endoplasmic reticulum (ER) is a key organelle fundamental for the maintenance of cellular homeostasis and the determination of cell fate under stress conditions. Reticulon-1C (RTN-1C) is a member of the reticulon family proteins localized primarily on the ER membrane and known to regulate ER structure and function. Several cellular processes depend on the structural and functional crosstalk between different organelles, particularly on the endoplasmic reticulum and mitochondria. These dynamic contacts, called mitochondria-associated ER membranes (MAMs), are essential for the maintenance of mitochondrial structure and participate in lipid and calcium exchanges between the two organelles. In this study we investigated the impact of RTN-1C modulation on mitochondrial dynamics. We demonstrate that RTN-1C controls mitochondrial structure and function affecting intracellular Ca2+ homeostasis and lipid exchange between ER and mitochondria. We propose that these events depend on RTN-1C involvement in the regulation of ER-mitochondria cross-talk and define a role for RTN-1C in maintaining the function of contacts between the two organelles.
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Reali
authors.affiliation: European Centre for Brain Research, IRCSS Santa Lucia Foundation, Via del Fosso di Fiorano 64, 00143 Rome, Italy.
authors.forename: Bisan
authors.initials: B
authors.lastname: Mehdawy
authors.affiliation: National Institute for Infectious Diseases, IRCCS 'L. Spallanzani', Via Portuense, 00149 Rome, Italy.
authors.forename: Roberta
authors.initials: R
authors.lastname: Nardacci
authors.affiliation: Cell Stress and Survival Unit, Danish Cancer Society Research Center, Strandboulevarden 49, 2100 Copenhagen, Denmark.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Filomeni
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Risuglia
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Rossin
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy; National Institute for Infectious Diseases, IRCCS 'L. Spallanzani', Via Portuense, 00149 Rome, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Antonioli
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy; National Institute for Infectious Diseases, IRCCS 'L. Spallanzani', Via Portuense, 00149 Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Marsella
authors.affiliation: National Institute for Infectious Diseases, IRCCS 'L. Spallanzani', Via Portuense, 00149 Rome, Italy; Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Lecce, Italy.
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Fimia
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy; National Institute for Infectious Diseases, IRCCS 'L. Spallanzani', Via Portuense, 00149 Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Piacentini
authors.affiliation: Department of Biology, University of Rome 'Tor Vergata', Via della Ricerca Scientifica, 00133 Rome, Italy. Electronic address: federica.di.sano@uniroma2.it.
authors.forename: Federica
authors.initials: F
authors.lastname: Di Sano
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000502:physiology; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism; D063154:Mitochondrial Dynamics / Q000187:drug effects / Q000235:genetics; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C081781:RTN1 protein, human
keywords: Endoplasmic reticulum; MAM; Mitochondria; RTN-1C