title: One-step formation and sterilization of gellan and hyaluronan nanohydrogels using autoclave.
abstract: The sterilization of nanoparticles for biomedical applications is one of the challenges that must be faced in the development of nanoparticulate systems. Usually, autoclave sterilization cannot be applied because of stability concerns when polymeric nanoparticles are involved. This paper describes an innovative method which allows to obtain, using a single step autoclave procedure, the preparation and, at the same time, the sterilization of self-assembling nanohydrogels (NHs) obtained with cholesterol-derivatized gellan and hyaluronic acid. Moreover, by using this approach, NHs, while formed in the autoclave, can be easily loaded with drugs. The obtained NHs dispersion can be lyophilized in the presence of a cryoprotectant, leading to the original NHs after re-dispersion in water.
authors.affiliation: Department of Drug Chemistry and Technologies, "Sapienza" University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.
authors.forename: Elita
authors.initials: E
authors.lastname: Montanari
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: De Rugeriis
authors.forename: Chiara
authors.initials: C
authors.lastname: Di Meo
authors.forename: Roberta
authors.initials: R
authors.lastname: Censi
authors.forename: Tommasina
authors.initials: T
authors.lastname: Coviello
authors.forename: Franco
authors.initials: F
authors.lastname: Alhaique
authors.forename: Pietro
authors.initials: P
authors.lastname: Matricardi
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers; D015195:Drug Design; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D020100:Hydrogels; D011108:Polymers; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D014867:Water; C048288:gellan gum; D006820:Hyaluronic Acid; D002784:Cholesterol