title: Evaluation of the cell cytotoxicity and DNA/BSA binding and cleavage activity of some dioxidovanadium(V) complexes containing aroylhydrazones.
abstract: Three dioxidovanadium(V) complexes [VO2L(1-3)] (1-3) [HL(1)=1-napthoyl hydrazone of 2-acetyl pyridine, HL(2)=2-furoyl hydrazone of 2-acetyl pyridine and H2L(3)=isonicotinoyl hydrazone of 2-hydroxy benzaldehyde] have been reported. All the complexes were characterized by various spectroscopy (IR, UV-visible and NMR) and the molecular structures of 1 and 2 were characterized by single crystal X-ray diffraction technique. Structural report established five-coordinate geometries, distorted toward square pyramidal for each of 1 and 2, based on a tridentate -O,N,N coordinating anion and two oxido-O atoms. The experimental results show that the complexes interact with calf-thymus DNA (CT-DNA) possibly by a groove binding mode, with binding constants of ~10(5)M(-1). All complexes show good photo-induced cleavage of pUC19 supercoiled plasmid DNA with complex 1 showing the highest photo-induced DNA cleavage activity of ~68%. 1-3 also exhibit moderate binding affinity in the range of 10(3)-10(4)M(-1) towards bovine serum albumin (BSA), while all the complexes show good photo-induced BSA cleavage activity. Moreover the antiproliferative activity of all these complexes was studied, which reveal all compounds are significantly cytotoxic towards the HeLa cell line.
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India.
authors.forename: Subhashree P
authors.initials: SP
authors.lastname: Dash
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar 751 013, Odisha, India.
authors.forename: Alok K
authors.initials: AK
authors.lastname: Panda
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India.
authors.forename: Sagarika
authors.initials: S
authors.lastname: Pasayat
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India.
authors.forename: Sudarshana
authors.initials: S
authors.lastname: Majumder
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Bhubaneswar, Bhubaneswar 751 013, Odisha, India. Electronic address: abiswas@iitbbs.ac.in.
authors.forename: Ashis
authors.initials: A
authors.lastname: Biswas
authors.affiliation: Department of Chemistry, University of Washington, Box 351700, Seattle, WA 98195, USA.
authors.forename: Werner
authors.initials: W
authors.lastname: Kaminsky
authors.affiliation: Department of Life Science, National Institute of Technology, Rourkela 769008, Odisha, India.
authors.forename: Subhadip
authors.initials: S
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Life Science, National Institute of Technology, Rourkela 769008, Odisha, India.
authors.forename: Sujit K
authors.initials: SK
authors.lastname: Bhutia
authors.affiliation: Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. Electronic address: rupamdinda@nitrkl.ac.in.
authors.forename: Rupam
authors.initials: R
authors.lastname: Dinda
mesh_terms: D002470:Cell Survival / Q000187:drug effects*; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D053837:DNA Cleavage; D006367:HeLa Cells; D006801:Humans; D006835:Hydrazones / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D008024:Ligands; D015394:Molecular Structure; D011485:Protein Binding; D012545:Schiff Bases / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D014466:Ultraviolet Rays; D017968:Vanadium Compounds / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D056831:Coordination Complexes; D006835:Hydrazones; D008024:Ligands; D012545:Schiff Bases; D017968:Vanadium Compounds; D012710:Serum Albumin, Bovine; D004247:DNA; C427203:calf thymus DNA
keywords: BSA activity; Cytotoxicity; DNA binding and photo-induced cleavage; Dioxidovanadium(V) aroylhydrazone complexes; X-ray crystal structure