title: Application of a nitric oxide sensor in biomedicine.
abstract: In the present study, we describe the biochemical properties and effects of nitric oxide (NO) in intact and dysfunctional arterial and venous endothelium. Application of the NO electrochemical sensor in vivo and in vitro in erythrocytes of healthy subjects and patients with vascular disease are reviewed. The electrochemical NO sensor device applied to human umbilical venous endothelial cells (HUVECs) and the description of others NO types of sensors are also mentioned.
authors.affiliation: Instituto de Bioquímica, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal; E-Mails: jpedro.gla@gmail.com (J.P.L.A.); anarmsilva@fm.ul.pt (A.S.S.-H.).
authors.forename: Carlota
authors.initials: C
authors.lastname: Saldanha
authors.affiliation: Instituto de Bioquímica, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal; E-Mails: jpedro.gla@gmail.com (J.P.L.A.); anarmsilva@fm.ul.pt (A.S.S.-H.) ; Servico de Imunoalergologia, Centro Hospitalar Lisboa Norte-Hospital de Santa Maria, 1649-028 Lisboa, Portugal.
authors.forename: José Pedro
authors.initials: JP
authors.lastname: Lopes de Almeida
authors.affiliation: Instituto de Bioquímica, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal; E-Mails: jpedro.gla@gmail.com (J.P.L.A.); anarmsilva@fm.ul.pt (A.S.S.-H.).
authors.forename: Ana Santos
authors.initials: AS
authors.lastname: Silva-Herdade
keywords: endothelium erythrocyte nitric oxide; microelectrode