title: Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.
abstract: The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
authors.affiliation: Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.|Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Susan N
authors.initials: SN
authors.lastname: Christo
authors.affiliation: Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.|Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.|Robinson Research Institute and School of Paediatrics and Reproductive Health, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Kerrilyn R
authors.initials: KR
authors.lastname: Diener
authors.affiliation: Graduate School of Biomedical Engineering, University of New South Wales, Kensington, NSW, 2052, Australia.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Nordon
authors.affiliation: Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.|Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.|Cancer Clinical Trials Unit, Royal Adelaide Hospital, Adelaide, SA 5000, Australia.|School of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Brown
authors.affiliation: Ian Wark Research Institute, University of South Australia, Adelaide, SA 5095, Australia.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Griesser
authors.affiliation: Mawson Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Krasimir
authors.initials: K
authors.lastname: Vasilev
authors.affiliation: Barbara Hardy Institute, University of South Australia, Adelaide, SA 5000, Australia.
authors.forename: Farid C
authors.initials: FC
authors.lastname: Christo
authors.affiliation: Experimental Therapeutics Laboratory, Hanson Institute, Adelaide, SA 5000, Australia.|Sansom Institute and School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000, Australia.|School of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Hayball
mesh_terms: D000818:Animals; D018106:CD28 Antigens / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D020013:Calcium Signaling; D005453:Fluorescence; D008024:Ligands; D008213:Lymphocyte Activation / Q000276:immunology*; D008810:Mice, Inbred C57BL; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; D008024:Ligands; D011948:Receptors, Antigen, T-Cell