title: Prevalence of Scedosporium species and Lomentospora prolificans in patients with cystic fibrosis in a multicenter trial by use of a selective medium.
abstract: OBJECTIVE
  Detection of hyphomycetes of the Scedosporium apiospermum complex and Lomentospora prolificans (Sac-Lp) is not yet standardized. Prevalence rates in patients with cystic fibrosis (CF) and the resistance pattern of these pathogens in Germany are unknown.
  
  
  METHODS
  In a one-year prospective study 11 laboratories used a selective medium for isolation of Sac-Lp, examining >11,600 respiratory samples from 2346 patients with CF. Isolates were identified by molecular methods and tested for susceptibility to antifungal drugs.
  
  
  RESULTS
  The prevalence of Sac-Lp in patients with CF in Germany varied from 0.0 to 10.5% (mean: 3.1%) among the clinical centres. The benefit of the selective medium SceSel(+) compared to standard media for fungi was documented for >5000 samples. High antifungal resistance was detected in the S. apiospermum complex, and the multiresistance of L. prolificans was confirmed.
  
  
  CONCLUSIONS
  Microbiology laboratories should be aware of these resistant species in patients with CF and consider using a selective medium.
authors.affiliation: Medizinische Hochschule Hannover, Medizinische Mikrobiologie und Krankenhaushygiene, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: Sedlacek.Ludwig@mh-hannover.de.
authors.forename: L
authors.initials: L
authors.lastname: Sedlacek
authors.affiliation: Labor Berlin - Charité Vivantes GmbH, Mikrobiologie/Mykologie, Augustenburger Platz 1, 13353 Berlin, Germany. Electronic address: barbara.graf@laborberlin.com.
authors.forename: B
authors.initials: B
authors.lastname: Graf
authors.affiliation: Christiane Herzog-Zentrum/Mukoviszidose, Klinik für Pädiatrie m.S. Pneumologie u. Immunologie, Sylter Str. 2, 13353 Berlin, Germany. Electronic address: carsten.schwarz@charite.de.
authors.forename: C
authors.initials: C
authors.lastname: Schwarz
authors.affiliation: Universitätsklinikum Erlangen, Mikrobiologisches Institut, Wasserturmstraße 3-5, 91054 Erlangen, Germany. Electronic address: frauke.albert@labor-limbach-nuernberg.de.
authors.forename: F
authors.initials: F
authors.lastname: Albert
authors.affiliation: Universitätsklinikum Tübingen, Institut für Medizinische Mikrobiologie und Hygiene, Elfriede-Aulhorn-Straße 6, 72076 Tübingen, Germany. Electronic address: silke.peter@med.uni-tuebingen.de.
authors.forename: S
authors.initials: S
authors.lastname: Peter
authors.affiliation: Max von Pettenkofer-Institut, Medizinische Mikrobiologie, Pettenkoferstraße 9a, 80336 München, Germany. Electronic address: b.wuerstl@hygconcept.de.
authors.forename: B
authors.initials: B
authors.lastname: Würstl
authors.affiliation: Helios Klinik Emil von Behring, Institut für Mikrobiologie, Immunologie und Labormedizin, Walterhöferstraße 11, 14165 Berlin, Germany. Electronic address: sonja.wagner@helios-kliniken.de.
authors.forename: S
authors.initials: S
authors.lastname: Wagner
authors.affiliation: Universitätskliniken des Saarlandes, Institut für Med. Mikrobiologie und Hygiene, Kirrberger Straße, Gebäude 43, 66421 Homburg/Saar, Germany. Electronic address: michael.klotz@uks.eu.
authors.forename: M
authors.initials: M
authors.lastname: Klotz
authors.affiliation: Städtisches Klinikum Karlsruhe, Zentralinstitut für Laboratoriumsmedizin, Mikrobiologie und Transfusionsmedizin, Moltkestraße 90, 76133 Karlsruhe, Germany. Electronic address: andrea.becker@klinikum-karlsruhe.de.
authors.forename: A
authors.initials: A
authors.lastname: Becker
authors.affiliation: RWTH Aachen, University Hospital, Institut für Med. Mikrobiologie, Pauwelsstraße 30, 52074 Aachen, Germany. Electronic address: ghaase@ukaachen.de.
authors.forename: G
authors.initials: G
authors.lastname: Haase
authors.affiliation: Dr. v. Haunersches Kinderspital, Bakteriologisches Labor, Ludwig Maximilian Universität, Lindwurmstr. 4, 80337 München, Germany. Electronic address: gudrun.laniado@med.uni-muenchen.de.
authors.forename: G
authors.initials: G
authors.lastname: Laniado
authors.affiliation: Universitätsklinikum Münster, Institut für Med. Mikrobiologie, Domagkstraße 10, 48149 Münster, Germany. Electronic address: kahl@uni-muenster.de.
authors.forename: B
authors.initials: B
authors.lastname: Kahl
authors.affiliation: Medizinische Hochschule Hannover, Medizinische Mikrobiologie und Krankenhaushygiene, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: suerbaum.sebastian@mh-hannover.de.
authors.forename: S
authors.initials: S
authors.lastname: Suerbaum
authors.affiliation: Robert Koch-Institut, FG 16 Mykologie, Nordufer 20, 13353 Berlin, Germany. Electronic address: seiboldm@rki.de.
authors.forename: M
authors.initials: M
authors.lastname: Seibold
authors.affiliation: Robert Koch-Institut, FG 16 Mykologie, Nordufer 20, 13353 Berlin, Germany. Electronic address: tintelnotk@rki.de.
authors.forename: K
authors.initials: K
authors.lastname: Tintelnot
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D003470:Culture Media / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000150:complications / Q000453:epidemiology / Q000382:microbiology; D025141:Drug Resistance, Fungal; D005260:Female; D005858:Germany / Q000453:epidemiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods; D009181:Mycoses / Q000175:diagnosis / Q000453:epidemiology / Q000209:etiology / Q000382:microbiology; D015995:Prevalence; D011446:Prospective Studies; D021681:Scedosporium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification
chemical_list: D000935:Antifungal Agents; D003470:Culture Media
keywords: Cystic fibrosis; Germany; Lomentospora; Prevalence; Scedosporium