title: Insulin-like growth factor-1 endues monocytes with immune suppressive ability to inhibit inflammation in the intestine.
abstract: The pathogenesis of some chronic inflammation such as inflammatory bowel disease is unclear. Insulin-like growth factor-1 (IGF1) has active immune regulatory capability. This study aims to investigate into the mechanism by which IGF1 modulates the monocyte (Mo) properties to inhibit immune inflammation in the intestine. In this study, the production of IGF1 by intestinal epithelial cells was evaluated by real time RT-PCR and Western blotting. Mos were analyzed by flow cytometry. A mouse colitis model was created with trinitrobenzene sulfonic acid. The results showed that mouse IECs produced IGF1, which could be up regulated by exposure to CpG-ODN (CpG-oligodeoxynueleotides) in the culture. Culture the CpG-ODN-primed IEC cells and Mos or exposure of Mos to IGF1 in the culture induced the Mos to express IL-10. The IGF1-primed Mos showed the immune suppressive effect on inhibiting the immune inflammation in the mouse colon. In conclusion, the IGF1-primed Mos are capable of suppressing immune inflammation in the intestine.
authors.affiliation: 1] Department of Gastroenterology, The Shanghai Tenth People's Hospital of Tongji University, Shanghai, 200072, China [2] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Rong-Ti
authors.initials: RT
authors.lastname: Ge
authors.affiliation: Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine and State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen, 518060, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mo
authors.affiliation: Department of Gastroenterology, The Shanghai Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Ruijin
authors.initials: R
authors.lastname: Wu
authors.affiliation: Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Huan-Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine and State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen, 518060, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The Shanghai Tenth People's Hospital of Tongji University, Shanghai, 200072, China.
authors.forename: Zhanju
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 1] Shenzhen Key Laboratory of Allergy &Immunology, Shenzhen University School of Medicine and State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen, 518060, China [2] Brain Body Institute, McMaster University, Hamilton, ON, Canada L8N 4A6.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D003092:Colitis / Q000276:immunology / Q000473:pathology; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007165:Immunosuppression Therapy; D007249:Inflammation / Q000276:immunology* / Q000473:pathology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism; D007422:Intestines / Q000276:immunology* / Q000473:pathology*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology; D010641:Phenotype; D017526:Receptor, IGF Type 1 / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D014302:Trinitrobenzenesulfonic Acid
chemical_list: C408982:CPG-oligonucleotide; D018950:Lipopolysaccharide Receptors; D009838:Oligodeoxyribonucleotides; D016753:Interleukin-10; D007334:Insulin-Like Growth Factor I; D014302:Trinitrobenzenesulfonic Acid; D017526:Receptor, IGF Type 1