title: OsARF16, a transcription factor regulating auxin redistribution, is required for iron deficiency response in rice (Oryza sativa L.).
abstract: Plant response to iron deficiency is the most important feature for survival in Fe-limited soils. Several phytohormones, including auxin, are involved in iron uptake and homeostasis. However, the mechanisms behind how auxin participates in the iron deficiency response in rice are largely unknown. We found that OsARF16 was involved in the iron deficiency response and the induction of iron deficiency response genes. Most Fe-deficient symptoms could be partially restored in the osarf16 mutant, including dwarfism, photosynthesis decline, a reduction in iron content and root system architecture (RSA) regulation. OsARF16 expression was induced in the roots and shoots by Fe deprivation. Restoration of the phenotype could also be mimicked by 1-NOA, an auxin influx inhibitor. Furthermore, the qRT-PCR data indicated that the induction of Fe-deficiency response genes by iron deficiency was more compromised in the osarf16 mutant than in Nipponbare. In conclusion, osarf16, an auxin insensitive mutant, was involved in iron deficiency response in rice. Our results reveal a new biological function for OsARF16 and provide important information on how ARF-medicated auxin signaling affects iron signaling and the iron deficiency response. This work may help us to improve production or increased Fe nutrition of rice to iron deficiency by regulating auxin signaling.
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: shencj@hznu.edu.cn.
authors.forename: Chenjia
authors.initials: C
authors.lastname: Shen
authors.affiliation: Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
authors.forename: Runqing
authors.initials: R
authors.lastname: Yue
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: whz62@163.com.
authors.forename: Huizhong
authors.initials: H
authors.lastname: Wang
mesh_terms: D001692:Biological Transport; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D012275:Oryza / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D007501:Iron
keywords: 1-NOA; Auxin; Auxin response factor; Iron deficiency response; OsARF16; Phytohormones