title: Human cytomegalovirus miR-US33-5p inhibits viral DNA synthesis and viral replication by down-regulating expression of the host Syntaxin3.
abstract: During infection with human cytomegalovirus (HCMV), overexpression of hcmv-miR-US33 can inhibit the lytic viral replication and down-regulate US29 mRNA. However, it remains unknown whether inhibition of viral replication by miR-US33 is mediated by down-regulation of expression of US29 or another host gene. Here, we identified the host gene Syntaxin3 (STX3) to be a direct target of hcmv-miR-US33-5p using Hybrid-PCR and luciferase-reporter assays. It was further demonstrated that the levels of STX3 protein were down-regulated in hcmv-miR-US33-5p-overexpressing cells. Experiments with STX3-specific siRNA, or with an inhibitor of hcmv-miR-US33-5p confirmed that hcmv-miR-US33-5p-mediated inhibition of HCMV DNA synthesis and of viral replication are specifically mediated by down-regulation of STX3 expression.
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China. Electronic address: ruanq@sj-hospital.org.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D003587:Cytomegalovirus / Q000502:physiology*; D004261:DNA Replication; D004279:DNA, Viral; D015536:Down-Regulation; D057809:HEK293 Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D050765:Qa-SNARE Proteins / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D012367:RNA, Viral / Q000502:physiology*; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D035683:MicroRNAs; D050765:Qa-SNARE Proteins; D012367:RNA, Viral
keywords: DNA synthesis; Down-regulation; Human cytomegalovirus; Syntaxin3; Virus replication; hcmv-miR-US33-5p