title: Tissue specific dysregulated protein subnetworks in type 2 diabetic bladder urothelium and detrusor muscle.
abstract: Diabetes mellitus is well known to cause bladder dysfunction; however, the molecular mechanisms governing this process and the effects on individual tissue elements within the bladder are poorly understood, particularly in type 2 diabetes. A shotgun proteomics approach was applied to identify proteins differentially expressed between type 2 diabetic (TallyHo) and control (SWR/J) mice in the bladder smooth muscle and urothelium, separately. We were able to identify 1760 nonredundant proteins from the detrusor smooth muscle and 3169 nonredundant proteins from urothelium. Pathway and network analysis of significantly dysregulated proteins was conducted to investigate the molecular processes associated with diabetes. This pinpointed ERK1/2 signaling as a key regulatory node in the diabetes-induced pathophysiology for both tissue types. The detrusor muscle samples showed diabetes-induced increased tissue remodeling-type events such as Actin Cytoskeleton Signaling and Signaling by Rho Family GTPases. The diabetic urothelium samples exhibited oxidative stress responses, as seen in the suppression of protein expression for key players in the NRF2-Mediated Oxidative Stress Response pathway. These results suggest that diabetes induced elevated inflammatory responses, oxidative stress, and tissue remodeling are involved in the development of tissue specific diabetic bladder dysfunctions. Validation of signaling dysregulation as a function of diabetes was performed using Western blotting. These data illustrated changes in ERK1/2 phosphorylation as a function of diabetes, with significant decreases in diabetes-associated phosphorylation in urothelium, but the opposite effect in detrusor muscle. These data highlight the importance of understanding tissue specific effects of disease process in understanding pathophysiology in complex disease and pave the way for future studies to better understand important molecular targets in reversing bladder dysfunction.
authors.affiliation: From the ‡Center for Proteomics and Bioinformatics.
authors.forename: Sara E
authors.initials: SE
authors.lastname: Tomechko
authors.affiliation: §Urology Institute, University Hospitals Case Medical Center and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106 guiming.liu@case.edu.
authors.forename: Guiming
authors.initials: G
authors.lastname: Liu
authors.affiliation: §Urology Institute, University Hospitals Case Medical Center and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106.
authors.forename: Mingfang
authors.initials: M
authors.lastname: Tao
authors.affiliation: From the ‡Center for Proteomics and Bioinformatics.
authors.forename: Daniela
authors.initials: D
authors.lastname: Schlatzer
authors.affiliation: §Urology Institute, University Hospitals Case Medical Center and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106.
authors.forename: C Thomas
authors.initials: CT
authors.lastname: Powell
authors.affiliation: §Urology Institute, University Hospitals Case Medical Center and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
authors.affiliation: From the ‡Center for Proteomics and Bioinformatics.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Chance
authors.affiliation: §Urology Institute, University Hospitals Case Medical Center and Department of Urology, Case Western Reserve University School of Medicine, Cleveland, Ohio, 44106.
authors.forename: Firouz
authors.initials: F
authors.lastname: Daneshgari
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D008297:Male; D051379:Mice; D009130:Muscle, Smooth / Q000378:metabolism*; D009928:Organ Specificity; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D001743:Urinary Bladder / Q000166:cytology* / Q000378:metabolism; D019459:Urothelium / Q000378:metabolism*
chemical_list: D020543:Proteome