title: Photochemical properties of gemifloxacin: a laser flash photolysis study.
abstract: The photochemical properties of gemifloxacin (GEFX), a fluoroquinolone antibacterial drug that exhibits phototoxicity toward biological substrates, were studied in aqueous solutions by laser flash photolysis (LFP) and pulse radiolysis. GEFX triplet state ((3)GEFX(∗)) absorption spectra showed maximum absorption at 510nm. (3)GEFX(∗) was quenched by naproxen (NAP) via energy transfer with a rate constant of 1.2×10(8)dm(3)mol(-1)s(-1). The energy of (3)GEFX(∗) was 266kJmol(-1) and the transient absorption spectra showed direct evidence of electron transfer from 2'-deoxyguanosine-5'-monophosphate, N,N,N',N'-tetramethyl-p-phenylenediamine, and tryptophan to (3)GEFX(∗) with bimolecular reaction rate constants of 4.1×10(6), 2.0×10(7), and 2.2×10(7)dm(3)mol(-1)s(-1), respectively. The rate constants for reactions of GEFX with OH, eaq(-) were found to be 1.5×10(10) and 1.4×10(10)dm(3)mol(-1)s(-1), respectively. The mechanisms and products of the photosensitive damage of lysozyme were related to the GEFX concentration, irradiation time, and ambient conditions.
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; University of Chinese Academy of Science, Beijing 100049, China.
authors.forename: Yulie
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Yancheng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; University of Chinese Academy of Science, Beijing 100049, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Li
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: wangwenfeng@sinap.ac.cn.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Wang
mesh_terms: D003848:Deoxyguanine Nucleotides / Q000737:chemistry; D004579:Electron Transport; D024841:Fluoroquinolones / Q000737:chemistry*; D000077735:Gemifloxacin; D017665:Hydroxyl Radical / Q000737:chemistry; D007700:Kinetics; D007834:Lasers; D009287:Naphthyridines / Q000737:chemistry*; D010782:Photolysis; D013772:Tetramethylphenylenediamine / Q000737:chemistry; D014364:Tryptophan / Q000737:chemistry
chemical_list: D003848:Deoxyguanine Nucleotides; D024841:Fluoroquinolones; D009287:Naphthyridines; D017665:Hydroxyl Radical; C007257:2'-deoxyguanosine 5'-phosphate; D014364:Tryptophan; D000077735:Gemifloxacin; D013772:Tetramethylphenylenediamine