title: A role for adaptor protein complex 1 in protein targeting to rhoptry organelles in Plasmodium falciparum.
abstract: The human malaria parasite Plasmodium falciparum possesses sophisticated systems of protein secretion to modulate host cell invasion and remodeling. In the present study, we provide insights into the function of the AP-1 complex in P. falciparum. We utilized GFP fusion constructs for live cell imaging, as well as fixed parasites in immunofluorescence analysis, to study adaptor protein mu1 (Pfμ1) mediated protein trafficking in P. falciparum. In trophozoites Pfμ1 showed similar dynamic localization to that of several Golgi/ER markers, indicating Golgi/ER localization. Treatment of transgenic parasites with Brefeldin A altered the localization of Golgi-associated Pfμ1, supporting the localization studies. Co-localization studies showed considerable overlap of Pfμ1 with the resident rhoptry proteins, rhoptry associated protein 1 (RAP1) and Cytoadherence linked asexual gene 3.1 (Clag3.1) in schizont stage. Immunoprecipitation experiments with Pfμ1 and PfRAP1 revealed an interaction, which may be mediated through an intermediate transmembrane cargo receptor. A specific role for Pfμ1 in trafficking was suggested by treatment with AlF4, which resulted in a shift to a predominantly ER-associated compartment and consequent decrease in co-localization with the Golgi marker GRASP. Together, these results suggest a role for the AP-1 complex in rhoptry protein trafficking in P. falciparum.
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: K M
authors.initials: KM
authors.lastname: Kaderi Kibria
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Khushboo
authors.initials: K
authors.lastname: Rawat
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Christen M
authors.initials: CM
authors.lastname: Klinger
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Datta
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Panchal
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Shailja
authors.initials: S
authors.lastname: Singh
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Gayatri R
authors.initials: GR
authors.lastname: Iyer
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India.
authors.forename: Inderjeet
authors.initials: I
authors.lastname: Kaur
authors.affiliation: Department of Bioscience and Biotechnology, Banasthali University, Banasthali-304022, Rajasthan, India.
authors.forename: Veena
authors.initials: V
authors.lastname: Sharma
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada. Electronic address: dacks@ualberta.ca.
authors.forename: Joel B
authors.initials: JB
authors.lastname: Dacks
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India. Electronic address: amohd@icgeb.res.in.
authors.forename: Asif
authors.initials: A
authors.lastname: Mohmmed
authors.affiliation: Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India. Electronic address: pawanm@icgeb.res.in.
authors.forename: Pawan
authors.initials: P
authors.lastname: Malhotra
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000502:physiology*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000469:parasitology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D010963:Plasmodium falciparum / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D033961:Adaptor Protein Complex 1; D008565:Membrane Proteins; D015800:Protozoan Proteins; C117001:ROP 1 protein, Toxoplasma gondii; D049452:Green Fluorescent Proteins
keywords: Adaptor protein complex-1 (AP-1); Clathrin; ER–Golgi network; Plasmodium falciparum; Trafficking; Vesicular trafficking