title: Effect of dissolved inorganic carbon on β-carotene and fatty acid production in Dunaliella sp.
abstract: This study aimed to explore the effect of sodium bicarbonate (0-200 mM) on the production of β-carotene and lipid content in Dunaliella salina and Dunaliella bardawil. Total carotenoid and chlorophyll content were determined at regular intervals by a UV-VIS spectrophotometer. The β-carotene and lipid contents were analyzed using high-performance liquid chromatography (HPLC) and gas chromatography coupled with mass spectrometry (GC-MS). The HPLC results revealed a twofold increase of β-carotene in D. salina and D. bardawil cultures grown with sodium bicarbonate. Moreover, total fatty acid profiles from GC-MS indicated a maximum relative percentage of saturated fatty acids (tetradecanoic acid, 10,13-diethyl, methyl ester and methyl 16-methyl-heptadecanoate) compared to polyunsaturated fatty acids in both algae. Our results indicate that the optimum concentration of bicarbonate (100 to 150 mM) was required to stimulate a positive effect on β-carotene production as well as the lipid profile in Dunaliella sp.
authors.affiliation: School of Bio-Sciences and Technology, VIT University, Vellore, 632 014, Tamil Nadu, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Srinivasan
authors.forename: Velayutham Arumuga
authors.initials: VA
authors.lastname: Kumar
authors.forename: Dileep
authors.initials: D
authors.lastname: Kumar
authors.forename: Nachimuthu
authors.initials: N
authors.lastname: Ramesh
authors.forename: Subramanian
authors.initials: S
authors.lastname: Babu
authors.forename: Kodiveri Muthukalianan
authors.initials: KM
authors.lastname: Gothandam
mesh_terms: D002734:Chlorophyll / Q000032:analysis / Q000378:metabolism; D000460:Chlorophyta / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis / Q000096:biosynthesis*; D017693:Sodium Bicarbonate / Q000378:metabolism*; D019207:beta Carotene / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D005227:Fatty Acids; D019207:beta Carotene; D002734:Chlorophyll; D017693:Sodium Bicarbonate