title: The optimal extracting process, manufacturing technique and biological evaluation of Lithospermum erythrorhizon microcapsules.
abstract: Lithospermum erythrorhizon has been proved to be anti-inflammatory, by recent studies. This study extracts L. erythrorhizon with ethanol at various solid-liquid ratios (1:4, 1:6, 1:8, and 1:12), extraction temperatures (40°C, 50°C, and 60°C), and extraction times (4, 24 and 36h) in order to determine the optimal parameters. The optimal parameters are extracted and condensed into L. erythrorhizon extract; then the antibacterial property and cell compatibility of L. erythrorhizon extract are evaluated with various concentrations of L. erythrorhizon extract solution and different weights of L. erythrorhizon extract powder, respectively. The concentrations of solution are 0.1mg/ml, 0.5mg/ml, 1.0mg/ml, and 2.0mg/ml and ethanol is chosen as the solvent, and different weights of powder are varied as 0.1mg, 1.0mg, 2.0mg, and 10mg. The cell viability test and animal study are performed on L. erythrorhizon microcapsules. The experiment results show that sodium alginate/pectin L. erythrorhizon (SPL) microcapsules possess a 120-hour drug release. The results of cell viability and animal study show that the L. erythrorhizon microcapsules (SPL) have good cell viability (99%) and can help in the wound healing process (the wound size reduction reaches 91.3% on Day 11).
authors.affiliation: Institute of Biomedical Engineering and Materials Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan, ROC.
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Lou
authors.affiliation: Department of Dental Technology and Materials Science, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan, ROC.
authors.forename: Chiung-Yun
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung City 40724, Taiwan, ROC.
authors.forename: Zong-Han
authors.initials: ZH
authors.lastname: Wu
authors.affiliation: Laboratory of Fiber Application and Manufacturing, Department of Fiber and Composite Materials, Feng Chia University, Taichung City 40724, Taiwan, ROC; School of Chinese Medicine, China Medical University, Taichung 40402, Taiwan, ROC; Department of Fashion Design, Asia University, Taichung 41354, Taiwan, ROC. Electronic address: jhlin@fcu.edu.tw.
authors.forename: Jia-Horng
authors.initials: JH
authors.lastname: Lin
mesh_terms: D000464:Alginates / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002214:Capsules / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D029684:Lithospermum / Q000737:chemistry*; D008297:Male; D009285:Naphthoquinones / Q000008:administration & dosage / Q000737:chemistry; D010368:Pectins / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011208:Powders; D017207:Rats, Sprague-Dawley; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000464:Alginates; D000900:Anti-Bacterial Agents; D002214:Capsules; D006603:Hexuronic Acids; D009285:Naphthoquinones; D010936:Plant Extracts; D011208:Powders; C016101:shikonin; D010368:Pectins; D020723:Glucuronic Acid
keywords: Lithospermum erythrorhizon; Microcapsules; Pectin; Sodium alginate; Wound healing