title: Effects of glucosamine and nucleotide association on fibroblast: extracellular matrix gene expression.
abstract: Glucosamine (Gluc) is a drug used as an anti-inflammatory in moderate forms of knee arthrosis. A further off label use of Gluc is in the anti-aging treatments associated with Polideoxirybonucleotide (PDRN) through intra-dermal injection for a procedure called bio-stimulation. An unexpected effect on cultured dermal fibroblasts, during an experimental study on the gene activation in aesthetic bio-stimulation, was observed. The results have potential application in orthopaedic medical therapy. Fibroblast primary cultures were carried out, seeding cells on a layer of Gluc or PDRN alone or in combination for 24 h. Real Time-PCR was performed to investigate several gene expressions. The MMP13 and the IGF-I gene expression in fibroblast cultures were strongly inhibited after 24 h of incubation with the association of Gluc and PDRN, whereas Gluc and PDRN alone produced a modest inhibition of IGF-I and an activation of MMP13. MMP13 is present in osteoarthritic cartilage and this enzyme plays a significant role in cartilage collagen degradation. IGF1 is involved in growth and development and is successfully used in tissue-engineering for cartilage repair. Based on the reported data, we infer that the association of Gluc and PDRN has a potential application in cartilage therapy. Additional basic science and clinical studies are needed to confirm this preliminary report.
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Avantaggiato
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University di Bologna, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Palmieri
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carinci
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Trapella
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Sollazzo
authors.affiliation: Department of Interdisciplinary Surgery and Medicine, University Milano Bicocca, Monza, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Lauritano
mesh_terms: D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D005944:Glucosamine / Q000494:pharmacology*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D053509:Matrix Metalloproteinase 13 / Q000235:genetics; D011089:Polydeoxyribonucleotides / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D016326:Extracellular Matrix Proteins; D011089:Polydeoxyribonucleotides; D007334:Insulin-Like Growth Factor I; C507079:MMP13 protein, human; D053509:Matrix Metalloproteinase 13; D005944:Glucosamine