title: Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain.
abstract: The sensory circumventricular organs (CVOs) comprise the organum vasculosum of the lamina terminalis (OVLT), subfornical organ (SFO), and area postrema (AP) and lack the blood-brain barrier. The expression of Toll-like receptor 4 (TLR4) was seen at astrocytes throughout the sensory CVOs and at microglia in the AP and solitary nucleus around the central canal. The peripheral and central administration of lipopolysaccharide induced a similar pattern of nuclear translocation of STAT3. A microglia inhibitor minocycline largely suppressed lipopolysaccharide-induced astrocytic nuclear translocation of STAT3 in the OVLT and AP, but its effect was less in the SFO.
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Nakano
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Furube
authors.affiliation: The Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical University, Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Shoko
authors.initials: S
authors.lastname: Morita
authors.affiliation: The Department of Anatomy and Neuroscience, Faculty of Medicine, Nara Medical University, Shijo-cho, Kashihara, Nara 634-8521, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Wanaka
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Nakashima
authors.affiliation: Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address: smiyata@kit.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Miyata
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology* / Q000187:drug effects; D002135:Calcium-Binding Proteins / Q000378:metabolism; D066280:Circumventricular Organs / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004333:Drug Administration Routes; D005786:Gene Expression Regulation / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008840:Microfilament Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D008911:Minocycline / Q000494:pharmacology; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000378:metabolism*
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D008070:Lipopolysaccharides; D008840:Microfilament Proteins; D050796:STAT3 Transcription Factor; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4; D017493:Leukocyte Common Antigens; D008911:Minocycline
keywords: Astrocyte; Brain; Fever; Inflammation; Microglia