title: Generating iPSCs: translating cell reprogramming science into scalable and robust biomanufacturing strategies.
abstract: Induced pluripotent stem cells (iPSCs) have the potential to transform drug discovery and healthcare in the 21(st) century. However, successful commercialization will require standardized manufacturing platforms. Here we highlight the need to define standardized practices for iPSC generation and processing and discuss current challenges to the robust manufacture of iPSC products.
authors.affiliation: Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK.
authors.forename: Marli
authors.initials: M
authors.lastname: Silva
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
authors.forename: Laurence
authors.initials: L
authors.lastname: Daheron
authors.affiliation: The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK.
authors.forename: Hannah
authors.initials: H
authors.lastname: Hurley
authors.affiliation: TAP Biosystems, Royston, Hertfordshire, SG8 5WY, UK.
authors.forename: Kim
authors.initials: K
authors.lastname: Bure
authors.affiliation: The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Barker
authors.affiliation: The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Nuffield Orthopaedic Centre, University of Oxford, Oxford, OX3 7LD, UK.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Carr
authors.affiliation: Centre for Biological Engineering, Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Loughborough, LE11 3TU, UK.
authors.forename: David
authors.initials: D
authors.lastname: Williams
authors.affiliation: Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center of Regenerative Medicine, Dankook University, Cheonan 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University Graduate School, Cheonan 330-714, Republic of Korea; Department of Dental Biomaterials, School of Dentistry, Dankook University, Shinbu-dong, Cheonan 330-714, Republic of Korea.
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.affiliation: Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK.
authors.forename: Anna
authors.initials: A
authors.lastname: French
authors.affiliation: Ocular Biology and Therapeutics, Institute of Ophthalmology, University College London, London, EC1V 9EL, UK; Neuroscience Research Institute, University of California, Santa Barbara, CA 93106-5060, USA.
authors.forename: Pete J
authors.initials: PJ
authors.lastname: Coffey
authors.affiliation: Australian Institute for Bioengineering & Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia; School of Chemical Engineering, The University of Queensland, St. Lucia, QLD 4072, Australia; Materials Science and Engineering Division, CSIRO, Clayton, VIC 3169, Australia.
authors.forename: Justin J
authors.initials: JJ
authors.lastname: Cooper-White
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA.
authors.forename: Brock
authors.initials: B
authors.lastname: Reeve
authors.affiliation: New York Stem Cell Foundation, New York, NY 10023, USA.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Rao
authors.affiliation: Burnham Medical Research Institute, La Jolla, CA 92037, USA; Department of Pediatrics, University of California, San Diego, La Jolla, CA 92161, USA; Sanford Consortium for Regenerative Medicine, La Jolla, CA 92037, USA.
authors.forename: Evan Y
authors.initials: EY
authors.lastname: Snyder
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
authors.forename: Kelvin S
authors.initials: KS
authors.lastname: Ng
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
authors.forename: Benjamin E
authors.initials: BE
authors.lastname: Mead
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA; The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK.
authors.forename: James A
authors.initials: JA
authors.lastname: Smith
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Division of Biomedical Engineering, Department of Medicine, Center for Regenerative Therapeutics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA 02139, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Karp
authors.affiliation: Harvard Stem Cell Institute, Cambridge, MA 02138, USA; The Oxford-UCL Centre for the Advancement of Sustainable Medical Innovation, University of Oxford, Oxford, OX3 9DU, UK; Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, Nuffield Orthopaedic Centre, University of Oxford, Oxford, OX3 7LD, UK; Centre for Behavioural Medicine, UCL School of Pharmacy, University College London, London WC1H 9JP, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Brindley
authors.affiliation: Department of Biochemical Engineering, University College London, London, WC1H 0AH, UK; Department of Nanobiomedical Science and BK21 Plus NBM Global Research Center of Regenerative Medicine, Dankook University, Cheonan 330-714, Republic of Korea; Institute of Tissue Regeneration Engineering, Dankook University Graduate School, Cheonan 330-714, Republic of Korea. Electronic address: i.wall@ucl.ac.uk.
authors.forename: Ivan
authors.initials: I
authors.lastname: Wall
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D065150:Cellular Reprogramming; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D015203:Reproducibility of Results; D033581:Stem Cell Transplantation