title: PF2405, standardized fraction of Scutellaria baicalensis, ameliorates colitis in vitro and in vivo.
abstract: Standardized extraction procedures for herb are as important as their authentication to maintain their quality and ensure their safe use. We had prepared a standardized and purified Scutellaria baicalensis Georgi extract, PF2405, which was enriched with three major components, baicalein, oroxylin A and wogonin. In the present study, we investigated the potential anti-inflammatory effects of PF2405 in vitro and in two different experimental animal models of inflammatory bowel disease. Effect of PF2405 studied in tumor necrosis factor (TNF)-α-induced HT-29 cells in vitro. In vivo experimental colitis models were induced by administration of trinitrobenzene sulfonic acid (TNBS) or dextran sulfate sodium (DSS). PF2405 (50 μg/ml) decreased TNF-α-induced cyclooxygenase (COX)-2 expressions through inhibition of phosphorylation of c-Jun N-terminal kinases and p38 mitogen-activated protein kinase in HT-29 cells. Combination of baicalein (20 μg/ml), oroxylin A (8 μg/ml), and wogonin (2 μg/ml) markedly inhibits TNF-α-induced COX-2 expression when compared with individual components. PF2405 (25 mg/kg b.w.) treatment significantly reduced histopathological severity; suppressed expression of COX-2, TNF-α, and interleukin-1β in TNBS-induced mice. Moreover, PF2405 (25 mg/kg b.w.) has both potent preventive and therapeutic activities in DSS-induced colitis. Collectively, PF2405 shows prominent anti-inflammatory effect that can be used as a new therapeutic approach for intestinal inflammatory disorders.
authors.affiliation: Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Jiang
authors.forename: Geom Seog
authors.initials: GS
authors.lastname: Seo
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology; D016264:Dextran Sulfate; D005260:Female; D019073:HT29 Cells; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors; D051379:Mice; D008810:Mice, Inbred C57BL; D010766:Phosphorylation; D010936:Plant Extracts / Q000627:therapeutic use*; D031364:Scutellaria baicalensis / Q000737:chemistry*; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha
chemical_list: D000893:Anti-Inflammatory Agents; D052246:Cyclooxygenase 2 Inhibitors; D053583:Interleukin-1beta; C000605640:PF-2405; D010936:Plant Extracts; C499814:Scutellaria baicalensis extract; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid; D016264:Dextran Sulfate; D048670:MAP Kinase Kinase 4