title: Real-time monitoring of phosphorylation kinetics with self-assembled nano-oscillators.
abstract: Phosphorylation is a post-translational modification that is involved in many basic cellular processes and diseases, but is difficult to detect in real time with existing technologies. A label-free detection of phosphorylation is reported in real time with self-assembled nano-oscillators. Each nano-oscillator consists of a gold nanoparticle tethered to a gold surface with a molecular linker. When the nanoparticle is charged, the nano-oscillator can be driven into oscillation with an electric field and detected with a plasmonic imaging approach. The nano-oscillators measure charge change associated with phosphorylation of peptides attached onto a single nanoparticle, allowing us to study the dynamic process of phosphorylation in real time without antibodies down to a few molecules, from which Michaelis and catalytic rate constants are determined.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 (China).
authors.forename: Yimin
authors.initials: Y
authors.lastname: Fang
authors.forename: Shan
authors.initials: S
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Shan
authors.forename: Nongjian
authors.initials: N
authors.lastname: Tao
mesh_terms: D000595:Amino Acid Sequence; D001710:Biotin / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D007700:Kinetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D020349:Surface Plasmon Resonance
chemical_list: D010455:Peptides; D001710:Biotin; D006046:Gold
keywords: charge-based detection; nano-oscillators; phosphorylation; plasmonic imaging; real-time imaging