title: Measurement of Vitamin K1in Commercial Canola Cultivars from Growing Locations in North and South America Using High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
abstract: Plant oils, including canola oil, are considered to be major sources of vitamin K as the second most substantial contributor of vitamin K to the human diet. Green leafy vegetables are the largest source of vitamin K. However, the effects of environment and germplasm on vitamin K levels in harvested canola seed have not been extensively investigated. To better understand these relationships, harvested canola seed from a range of diverse cultivars grown in different geographical locations in North and South America was assessed for levels of vitamin K. The analytical method developed to perform this measurement was based on C30reversed-phase HPLC that could distinguish the biologically active trans-vitamin K1from the inactive cis-isomer. Results demonstrated that for the majority of the canola cultivars evaluated, those cultivated in the North American sites had higher average vitamin K1levels than those cultivated in the South American sites. Not all of the cultivars exhibited differences in response to the environment, suggesting that individual cultivar genetics also played a role in the variability of vitamin K1levels observed in canola seed. Results from this study suggest that cultivar and environmental effects influence vitamin K1levels in canola seed and provide a context to assess compositional variability of new cultivars.
authors.affiliation: EPL Bio Analytical Services, 9095 West Harristown Boulevard, Niantic, Illinois 62551, United States.
authors.forename: Fred A
authors.initials: FA
authors.lastname: Claussen
authors.affiliation: Monsanto Company, LLC, 800 North Lindbergh Boulevard, Creve Coeur, Missouri 63167, United States.
authors.forename: Mary L
authors.initials: ML
authors.lastname: Taylor
authors.affiliation: Monsanto Company, LLC, 800 North Lindbergh Boulevard, Creve Coeur, Missouri 63167, United States.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Breeze
authors.affiliation: Monsanto Company, LLC, 800 North Lindbergh Boulevard, Creve Coeur, Missouri 63167, United States.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
keywords: Brassica napus; HPLC-MS/MS; canola; high-performance liquid chromatography−tandem mass spectrometry; low erucic acid rapeseed; phylloquinone; vitamin K1