title: ASPicDB: a database web tool for alternative splicing analysis.
abstract: Alternative splicing (AS) is a basic molecular phenomenon that increases the functional complexity of higher eukaryotic transcriptomes. Indeed, through AS individual gene loci can generate multiple RNAs from the same pre-mRNA. AS has been investigated in a variety of clinical and pathological studies, such as the transcriptome regulation in cancer. In human, recent works based on massive RNA sequencing indicate that >95 % of pre-mRNAs are processed to yield multiple transcripts. Given the biological relevance of AS, several computational efforts have been done leading to the implementation of novel algorithms and specific specialized databases. Here we describe the web application ASPicDB that allows the recovery of detailed biological information about the splicing mechanism. ASPicDB provides powerful querying systems to interrogate AS events at gene, transcript, and protein levels. Finally, ASPicDB includes web visualization instruments to browse and export results for further off-line analyses.
authors.affiliation: Consorzio Interuniversitario CINECA, 00185, Rome, Italy.
authors.forename: Mattia
authors.initials: M
authors.lastname: D'Antonio
authors.forename: Tiziana
authors.initials: T
authors.lastname: Castrgnanò
authors.forename: Matteo
authors.initials: M
authors.lastname: Pallocca
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: D'Erchia
authors.forename: Ernesto
authors.initials: E
authors.lastname: Picardi
authors.forename: Graziano
authors.initials: G
authors.lastname: Pesole
mesh_terms: D000465:Algorithms; D017398:Alternative Splicing / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D030541:Databases, Genetic; D020407:Internet