title: [Inhibition effect of N-acetyl-seryl-aspartyl-lysyl-proline on myofibroblast differentiation of MRC-5 human fetal lung fibroblasts inuced by Ang II].
abstract: OBJECTIVE
  To explore the inhibition effect of N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) on myofibroblast differentiation of MRC-5 human fetal lung fibroblasts induced by angiotensin (Ang) II.
  
  
  METHODS
  The study was divided into 2 step: (1) MRC-5 human fetal lung fibroblasts was induced for 48 h at different dose of Ang II and at different time point by 100 nmol/L Ang II. Then the expression of collagen type I and α-smooth muscle actin (α-SMA) were mesaured by western blot. (2) MRC-5 human fetal lung fibroblasts were divided into 4 group: (1) control, (2) Ang II, (3) Ang II+Ac-SDKP, (4) Ang II+8-Me-cAMP (a specific activator of Epac). The α-SMA expression was observed by immnocytochemical stain. The protein expression of collagen type I, α-SMA, serum response factor (SRF), myocardin-related transcription factor (MRTF)-A, exchange protein directly activated by cAMP (Epac) 1, 2 were measured by Westen blot.
  
  
  RESULTS
  Myofibroblast differentiation could be induced by Ang II from MRC-5 cells with a dose- and time-dependent manner. The up-regulation of SRF and MRTF-A were observed in MRC-5 cells induced by Ang II and accompanied with collagen I and α-SMA increased. Pre-treatment with 8-Me-cAMP or Ac-SDKP could attenuated all this changes induced by Ang II, and promoted the expression of Epac1.
  
  
  CONCLUSIONS
  Ac-SDKP can inhibit the myofibroblast differentiation of MRC-5 cells induced by Ang II via Epac1 activating.
authors.affiliation: Hebei United University, Tangshan 063000, China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Li
authors.forename: Shipu
authors.initials: S
authors.lastname: Du
authors.forename: Xinxin
authors.initials: X
authors.lastname: Xue
authors.forename: Dingjie
authors.initials: D
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.forename: Haijing
authors.initials: H
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Wei
authors.forename: Jingrui
authors.initials: J
authors.lastname: Tian
authors.affiliation: E-mail: fangyang1955@163.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
mesh_terms: D000199:Actins; D000804:Angiotensin II; D002454:Cell Differentiation / Q000187:drug effects*; D003094:Collagen; D024042:Collagen Type I; D000242:Cyclic AMP / Q000031:analogs & derivatives; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D008168:Lung / Q000166:cytology; D058628:Myofibroblasts / Q000187:drug effects*; D009842:Oligopeptides / Q000494:pharmacology*; D026362:Serum Response Factor; D015534:Trans-Activators
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D020662:Guanine Nucleotide Exchange Factors; C431575:MRTFA protein, human; D009842:Oligopeptides; C116122:RAPGEF3 protein, human; D026362:Serum Response Factor; D015534:Trans-Activators; D000804:Angiotensin II; D003094:Collagen; D000242:Cyclic AMP; C058504:goralatide