title: Aucubin prevents interleukin-1 beta induced inflammation and cartilage matrix degradation via inhibition of NF-κB signaling pathway in rat articular chondrocytes.
abstract: Proinflammatory cytokine interleukin-1β (IL-1β) plays a crucial role in the pathogenesis of Osteoarthritis (OA) by stimulating several mediators contributed to cartilage degradation. Aucubin, a natural compound derived from plants which has been shown to possess diverse biological activities including anti-inflammatory property, may benefit the IL-1β stimulated chondrocytes. The present study was aimed to investigate the effects of Aucubin on IL-1β stimulated rat chondrocytes. Rat chondrocytes were cultured and pretreated with Aucubin (1, 10, 20, 50μM), and then stimulated with or without IL-1β (10ng/ml). Gene and protein expression of MMP-3, MMP-9, MMP-13, cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) was determined by real-time PCR and Western blotting respectively. Nitric oxide (NO) production was quantified by Griess reagent. Phosphorylation and nuclear translocation of p65 were detected by western blotting and immunofluorescence, respectively. We found that Aucubin significantly reversed the elevated gene and protein expression of MMP-3, MMP-9, MMP-13, iNOS, COX-2 and the production of NO induced by IL-1β challenge in rat chondrocytes. Furthermore, Aucubin was able to suppress the IL-1β-mediated phosphorylation and nuclear translocation of p65, indicating Aucubin may possibly act via the NF-κB signaling pathway. The present study proposes that Aucubin may be a potential therapeutic choice in the treatment of OA due to its anti-inflammatory and chondroprotective features.
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: 151102352@qq.com.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: xgp19@163.com.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Xie
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: doctorqin@163.com.
authors.forename: Cheng-He
authors.initials: CH
authors.lastname: Qin
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: chengyirong2001@163.com.
authors.forename: Yi-Rong
authors.initials: YR
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: 280219927@qq.com.
authors.forename: Kai-Rui
authors.initials: KR
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: 151961353@qq.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of spinal surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: 435856869@qq.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital to Guangzhou Medical University, Guangzhou 510120, China. Electronic address: dwqdoctor@163.com.
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Dong
authors.affiliation: Department of Orthopaedics, 421 Hospital of People's Liberation Army, Guangzhou 510318, China. Electronic address: yangjun1267@sina.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics and Traumatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Electronic address: yubinortho@sina.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002358:Cartilage, Articular / Q000166:cytology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta; D057888:Iridoid Glucosides / Q000494:pharmacology*; D053509:Matrix Metalloproteinase 13 / Q000235:genetics / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D057888:Iridoid Glucosides; D016328:NF-kappa B; D012333:RNA, Messenger; C006650:aucubin; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D053509:Matrix Metalloproteinase 13; C507083:Mmp13 protein, rat; D019278:Matrix Metalloproteinase 3; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat
keywords: Aucubin; Chondrocyte; IL-1β; MMPs; Osteoarthritis