title: Contribution of IL-12A and IL-12B polymorphisms to Chlamydia trachomatis-specific cell-mediated immune responses.
abstract: Inherited variance in the IL-12B gene is associated with susceptibility to Chlamydia trachomatis-induced tubal factor infertility and disease severity. In this study, our aim was to discover how polymorphisms in IL-12-coding genes influence C. trachomatis-induced immune responses and IL-12 production. The study population consisted of 240 women. IL-12A and IL-12B single nucleotide polymorphisms (SNPs) were determined from isolated DNA using the Sequenom system with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. We studied lymphocyte proliferative (LP) responses to C. trachomatis strains E and F elementary bodies (EBs) and recombinant chlamydial heat-shock protein 60 (CHSP60) antigen. IL-12p40 and IL-12p70 levels were measured using the BD Flex Set method. We found a statistically significant association between the C. trachomatis EB antigen-specific LP response and the rs2853694 SNP (P = 0.02). Our study demonstrates that the IL-12 cytokine family is involved in C. trachomatis-specific immune responses. Moreover, C. trachomatis-induced IL-12 production and the IL-12B rs2853694 SNP partially explain individual variation in the C. trachomatis LP response.
authors.affiliation: National Institute for Health and Welfare, Oulu, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Öhman
authors.forename: A
authors.initials: A
authors.lastname: Natividad
authors.forename: R
authors.initials: R
authors.lastname: Bailey
authors.forename: J
authors.initials: J
authors.lastname: Ragoussis
authors.forename: L-L
authors.initials: LL
authors.lastname: Johnson
authors.forename: A
authors.initials: A
authors.lastname: Tiitinen
authors.forename: M
authors.initials: M
authors.lastname: Halttunen
authors.forename: J
authors.initials: J
authors.lastname: Paavonen
authors.forename: H-M
authors.initials: HM
authors.lastname: Surcel
mesh_terms: D000293:Adolescent; D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D018834:Chaperonin 60 / Q000276:immunology; D002690:Chlamydia Infections / Q000276:immunology*; D002692:Chlamydia trachomatis / Q000276:immunology*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics / Q000276:immunology; D007247:Infertility, Female / Q000382:microbiology; D053762:Interleukin-12 Subunit p40 / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D018834:Chaperonin 60; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40