title: Selective recognition of 5-hydroxytryptamine and dopamine on a multi-walled carbon nanotube-chitosan hybrid film-modiﬁed microelectrode array.
abstract: It is difﬁcult to determine dopamine (DA) and 5-hydroxytryptamine (5-HT) accurately because of the interference of ascorbic acid (AA) in vitro, which has a high concentration and can be oxidized at a potential close to DA and 5-HT at a conventional electrode, combined with the overlapping voltammetric signal of DA and 5-HT at a bare electrode. Herein, chitosan (CS) was used as a stabilizing matrix by electrochemical reaction, and multi-walled carbon nanotubes (MWCNTs) were modified onto the microelectrode array (MEA). The CS-MWCNT hybrid film-modified MEA was quite effective at simultaneously recognizing these species in a mixture and resolved the overlapping anodic peaks of AA, DA and 5-HT into three well-deﬁned oxidation peaks in differential pulse voltammetry (DPV) at -80 mV, 105 mV and 300 mV (versus Ag|AgCl), respectively. The linear responses were obtained in the range of 5 × 10(-6) M to 2 × 10(-4) M for DA (r = 0.996) and in the range of 1 × 10(-5) M to 3 × 10(-4) M for 5-HT (r = 0.999) using the DPV under the presence of a single substance. While DA coexisted with 5-HT in the interference of 3 × 10(-4) M AA, the linear responses were obtained in the range of 1 × 10(-5) M to 3 × 10(-4) M for selective molecular recognition of DA (r = 0.997) and 5-HT (r = 0.997) using the DPV. Therefore, this proposed MEA was successfully used for selective molecular recognition and determination of DA and 5-HT using the DPV, which has a potential application for real-time determination in vitro experiments.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. xuhuiren_2012@163.com.
authors.forename: Huiren
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. wangli2011iecas@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. jpluo@mail.ie.ac.cn.
authors.forename: Jinping
authors.initials: J
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. ylsong@mail.ie.ac.cn.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Song
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. jpluo@mail.ie.ac.cn.
authors.forename: Juntao
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. zhangsong0112@126.com.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics Chinese Academy of Sciences, Beijing 100190, China. xxcai@mail.ie.ac.cn.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis; D002384:Catalysis; D048271:Chitosan / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis*; D055664:Electrochemical Techniques; D006863:Hydrogen-Ion Concentration; D008839:Microelectrodes; D055616:Microtechnology; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D012701:Serotonin / Q000032:analysis*
chemical_list: D037742:Nanotubes, Carbon; D012701:Serotonin; D048271:Chitosan; D001205:Ascorbic Acid; D004298:Dopamine