title: ERBB3-mediated regulation of Bergmann glia proliferation in cerebellar lamination.
abstract: Cortical lamination is crucial for the assembly of cerebellar circuitry. In this process, granule neurons (GNs) migrate along Bergmann glia (BG), which are specialized astroglial cells, from the external granule layer to the internal granule layer. However, the molecular mechanisms underlying BG development are not well understood. Here, we show that GFAP::Cre;Erbb3(F/F) mice, which lack Erbb3 in both radial glia and neurons, exhibit impairments in balance and motor coordination. Cerebellar lamination is aberrant, with misplaced Purkinje neurons and GN clusters. These phenotypes were not observed in Math1::CreER(T2);Erbb3(F/F) mice, where the Erbb3 gene was deleted in GNs, suggesting involvement of non-neuronal Erbb3 in cerebellar lamination. Mechanistic studies indicate that ERBB3 is crucial for the proliferation of BG, which are required for GN migration. These observations identify a crucial role for ERBB3 in cerebellar lamination and reveal a novel mechanism that regulates BG development.
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Anupama
authors.initials: A
authors.lastname: Sathyamurthy
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Yin
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Arnab
authors.initials: A
authors.lastname: Barik
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA Charlie Norwood VA Medical Center, Augusta, GA 30904, USA.
authors.forename: Chengyong
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Jonathan C
authors.initials: JC
authors.lastname: Bean
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA Charlie Norwood VA Medical Center, Augusta, GA 30904, USA.
authors.forename: Dwight
authors.initials: D
authors.lastname: Figueiredo
authors.affiliation: Center for Biotechnology and Genomic Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Jin-Xiong
authors.initials: JX
authors.lastname: She
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA Charlie Norwood VA Medical Center, Augusta, GA 30904, USA Department of Neurology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Xiong
authors.affiliation: Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA Charlie Norwood VA Medical Center, Augusta, GA 30904, USA Department of Neurology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA lmei@gru.edu.
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation / Q000502:physiology*; D002531:Cerebellum / Q000166:cytology / Q000196:embryology*; D017931:DNA Primers / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D009457:Neuroglia / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000502:physiology*; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D020893:Receptor, ErbB-3 / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D020893:Receptor, ErbB-3
keywords: Bergmann glia; Cerebellum; ERBB3; Mouse