title: Optical properties of tissues quantified using morphological granulometry from phase-contrast images of thin tissue samples.
abstract: A method combining morphological granulometry with Mie theory to determine optical scattering in biological tissues was proposed. Otsu's method was applied to binarize phase-contrast images. Binary morphological granulometry was used to estimate size density distribution of the tissue samples based on the binary phase-contrast images. Our results showed that the optical parameters associated with light scattering in tissue could be quantitatively determined by combining size density distribution with Mie theory. It was suggested that this unique method could be used to characterize biological tissues for disease diagnosis.
authors.affiliation: Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, Fujian, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiovascular Surgery, Fujian Provincial Hospital, Fuzhou, Fujian, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Chen
authors.affiliation: Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, Fujian, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, Fujian, China Biophotonics Research Laboratory Department of Engineering and Physics, University of Central Oklahoma, Edmond, OK, USA.
authors.forename: Wei R
authors.initials: WR
authors.lastname: Chen
mesh_terms: D003198:Computer Simulation; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008027:Light; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D008954:Models, Biological; D009391:Nephelometry and Turbidimetry / Q000379:methods; D012031:Refractometry / Q000379:methods*; D012542:Scattering, Radiation
keywords: Mie theory; Morphological granulometry; optical scattering properties