title: [Identification of key markers of normal and pathogenic microbiota determining health of periodontium by NGS-sequencing 16S-rDNA libraries of periodontal swabs].
abstract: By using NGS-sequencing libraries of DNA from periodontal swabs with primers specific to V6 region of 16S rDNA prevalence of bacterial genera and species in periodontal microbiota of patients with aggressive periodontitis and healthy donors was analyzed. Six genera of putative periodontal protectors and eight periodontal pathogens were identified with respect to aggressive (but not chronic) periodontitis. Statistically relevant over-colonization by general Porphyromonas, Treponema, Synergistes, Tannerella, Filifactor, Ruminococcus, Parvimonas and Mycoplasma was found to be associated with the condition. From these, only three genera Porphyromonas, Treponema and Tannerella are traditionally considered as periodontal pathogens. Statistically confidential over-colonization by genus Veillonella was found in healthy patients. This genus should be considered as a relevant marker of a healthy periodontium. Genera Streptococcus, Bergeyella, Granulicatella, Kingella and Corynebacterium may be considered as putative periodontal protectors. Comparison of data of NGS-sequencing and real-time PCR demonstrated a good agreement if different PCR efficiency using independent primer pairs is taken into account.
authors.affiliation: FGBU "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut stomatologii i cheliustno-litsevoĭ khirurgii" Ministerstva zdravookhraneniia Rossiĭskoĭ Federatsii, Moskva; GBOU VPO Pervyĭ Moskovskiĭ gosudarstvennyĭ universitet im. I.M. Sechenova, Moskva.
authors.forename: O A
authors.initials: OA
authors.lastname: Zorina
authors.affiliation: FGBU "Tsentral'nyĭ nauchno-issledovatel'skiĭ institut stomatologii i cheliustno-litsevoĭ khirurgii" Ministerstva zdravookhraneniia Rossiĭskoĭ Federatsii, Moskva; GBOU VPO Pervyĭ Moskovskiĭ gosudarstvennyĭ universitet im. I.M. Sechenova, Moskva.
authors.forename: N B
authors.initials: NB
authors.lastname: Petrukhina
authors.affiliation: GBOU VPO Pervyĭ Moskovskiĭ gosudarstvennyĭ universitet im. I.M. Sechenova, Moskva.
authors.forename: A A
authors.initials: AA
authors.lastname: Basova
authors.affiliation: Institut biokhimicheskoĭ fiziki im. N.M. Émanuélia RAN, Moskva; Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva.
authors.forename: A V
authors.initials: AV
authors.lastname: Shibaeva
authors.affiliation: Kurskiĭ gosudarstvennyĭ universitet, Kursk.
authors.forename: E V
authors.initials: EV
authors.lastname: Trubnikova
authors.affiliation: Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva.
authors.forename: A B
authors.initials: AB
authors.lastname: Shevelev
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D008297:Male; D064307:Microbiota / Q000235:genetics*; D008875:Middle Aged; D010512:Periodontal Index; D010518:Periodontitis / Q000382:microbiology*; D010519:Periodontium / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S