title: Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro.
abstract: Regenerated skeletal muscles show less muscle damage after strenuous muscle exercise. The aim of the studies was to investigate if the regeneration is associated with reduced muscle creatine kinase (CK) efflux immediately after the exercise. Cryolesion was applied to the soleus muscle of 3-month-old C57BL/6J male mice. Then total CK efflux was assessed in vitro in the regenerated muscles without exercise or after 100 eccentric contractions. The same measurements were performed in the control muscles, which were not exposed to cryolesion. Regenerated muscles generated weaker (P < 0.05) twitches, but stronger (P < 0.05) 150-Hz and 300-Hz tetani with prolonged (P < 0.01) contraction times compared with the control muscles. There was no difference between regenerated and control muscles in the total CK efflux without exercise, but only control muscles showed an increase (P < 0.001) in the CK efflux after the exercise. Our results suggest that muscle regeneration is associated with modulation of contractile properties and improvement in muscle resistance to damage after eccentric exercise.
authors.affiliation: a Institute of Sports Sciences and Innovation, Lithuanian Sports University, Sporto 6, LT-44221 Kaunas, Lithuania.
authors.forename: Juozas
authors.initials: J
authors.lastname: Baltusnikas
authors.forename: Audrius
authors.initials: A
authors.lastname: Kilikevicius
authors.forename: Tomas
authors.initials: T
authors.lastname: Venckunas
authors.forename: Andrej
authors.initials: A
authors.lastname: Fokin
authors.forename: Arimantas
authors.initials: A
authors.lastname: Lionikas
authors.forename: Aivaras
authors.initials: A
authors.lastname: Ratkevicius
mesh_terms: D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*
chemical_list: D003402:Creatine Kinase
keywords: contractions avec étirement; cryolesion; cryolésion; dommage musculaire; dommage primaire; eccentric exercise; exercice pliométrique; lengthening contractions; mice; muscle damage; primary damage; repeated bout effect; répétition d’exercice; souris