title: BRCA1 and γH2AX as independent prognostic markers in oral squamous cell carcinoma.
abstract: Oral squamous cell carcinomas (OSCC) are believed to originate from sequential mutations that can develop as a consequence of genetic instability acquired over time. BRCA1 are linked to DNA recombination and repair processes, being of importance for its role in regulation of RAD51 and H2AX (γH2AX). The aim of this study was to investigate the relationship between BRCA1 expression status and evaluate its prognostic impact. We selected from 150 OSCC patients, and evaluated BRCA1 expression in OSCC by immunohistochemistry and qRT-PCR, comparing its expression with homologous recombination markers (RAD51, γH2AX and p53), clinicopathological and survival data. Expression of BRCA1 was observed in 61 cases (43.88%) and was related to tumor size (T stage) (p=0.001), and gender (p=0.017). mRNA from BRCA1 showed a borderline relationship with perineural invasion (p=0.053). BRCA1 [p=0.030; HR: 2.334 (C.I.: 1.087-5.012)], γH2AX [p=0.045; HR: 0.467 (C.I.: 0.222-0.628)] and gender [p=0.001; HR: 10.386 [(C.I.: 2.679-10.623)] were independent prognostic factors for DSS. BRCA1 and γH2AX expression by OSCC cells are associated with reduced overall survival time, independent of other variables in patients, as well as gender, and our findings shed some light about DSB markers in OSCC and its role as prognostic factors.
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil ; Department Anatomic Pathology, AC Camargo Cancer Center, Sao Paulo, Brazil.
authors.forename: Joao Paulo
authors.initials: JP
authors.lastname: Oliveira-Costa
authors.affiliation: Department of Oral Pathology, School of Dentistry, Vale do Rio Verde University (Unincor), Tres Coracoes, Brazil.
authors.forename: Lucinei Roberto
authors.initials: LR
authors.lastname: Oliveira
authors.affiliation: Department of Oral Pathology, School of Dentistry, Vale do Rio Verde University (Unincor), Tres Coracoes, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Zanetti
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Juliana Silva
authors.initials: JS
authors.lastname: Zanetti
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Giorgia Gobbi
authors.initials: GG
authors.lastname: da Silveira
authors.affiliation: Department Anatomic Pathology, AC Camargo Cancer Center, Sao Paulo, Brazil.
authors.forename: Marcilei Eliza
authors.initials: ME
authors.lastname: Chavichiolli Buim
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Sergio
authors.initials: S
authors.lastname: Zucoloto
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ribeiro-Silva
authors.affiliation: Department of Pathology and Forensic Medicine, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil.
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Soares
keywords: BRCA1; DNA damage; H2AX; oral squamous cell carcinoma; prognostic factor