title: Buckwheat Rutin Inhibits AngII-induced Cardiomyocyte Hypertrophy via Blockade of CaN-dependent Signal Pathway.
abstract: Buckwheat rutin has been found to be able to inhibit angiotensin II (AngII) - induced hypertrophy in cultured neonatal rat cardiomyocytes, but the mechanism remains uncertain. In this study, myocardial hypertrophy model was made by adding AngII to the medium of cardiac myocytes of neonatal rats; meanwhile, different concentrations of buckwheat rutin were applied to observe their effects. Intracellular Ca(2+) level was detected by Hitachi - 850 fluorospectrophotometer, calcineurin (CaN) activity was measured by colorimetric method, the expression of CaN protein was observed with immunocytochemistry, and the proto - oncogene c - fos mRNA expression was assessed with reverse transcription polymerase chain reaction (RT - PCR). Compared with control group, AngII could greatly stimulate the increase of intracellular Ca(2+) level, the activities and protein expression of cardiomyocytes CaN, and the expression of proto - oncogene c - fos mRNA in cultured neonatal rat cardiomyocytes, which could be effectively decreased by buckwheat rutin. Our results demonstrated that buckwheat rutin exhibited inhibitory effect on AngII - induced hypertrophy in cultured neonatal rat cardiomyocytes via Ca(2+) antagonism action thus block the CaN - dependent signal pathway.
authors.affiliation: Department of Pharmacology, College of Elementary Medicine , Hebei United University, Tangshan 063000, PR China .
authors.forename: Jin-Xiu
authors.initials: JX
authors.lastname: Chu
authors.affiliation: Tangshan Women and Children's Hospital Affiliated to Hebei United University, Tangshan 063000, PR China .
authors.forename: Guang-Min
authors.initials: GM
authors.lastname: Li
authors.affiliation: Department of Traditional Chinese Medicine , Hebei United University, Tangshan 063000, PR China .
authors.forename: Xiu-Juan
authors.initials: XJ
authors.lastname: Gao
authors.affiliation: Jitang College of Hebei United University, Tangshan 063000, PR China .
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, College of Elementary Medicine , Hebei United University, Tangshan 063000, PR China .
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Han
keywords: Angiotensin II; Buckwheat; Calcineurin; Cardiac hypertrophy; Rutin