title: Interaction of Aβ peptide with tubulin causes an inhibition of tubulin polymerization and the apoptotic death of cancer cells.
abstract: We report in this work that the Aβ peptide directly interacts with tubulin close to the vinblastine and GTP/GDP binding site, inhibits the tubulin polymerization rate, induces tubulin aggregation, causes cell shrinking, enhances Mad2, BubR1, p53, and p21 activation in MCF7 cells and induces the apoptotic death of A549, HeLa and MCF7 cells.
authors.affiliation: Chemistry Division, CSIR-Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata-700032, West Bengal, India. sghosh@iicb.res.in.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Saha
authors.forename: Saswat
authors.initials: S
authors.lastname: Mohapatra
authors.forename: Prashant
authors.initials: P
authors.lastname: Kurkute
authors.forename: Batakrishna
authors.initials: B
authors.lastname: Jana
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Mondal
authors.forename: Debmalya
authors.initials: D
authors.lastname: Bhunia
authors.forename: Subhajit
authors.initials: S
authors.lastname: Ghosh
authors.forename: Surajit
authors.initials: S
authors.lastname: Ghosh
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism*; D017209:Apoptosis; D001665:Binding Sites; D045744:Cell Line, Tumor; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D064246:Mad2 Proteins / Q000378:metabolism; D010455:Peptides; D058105:Polymerization; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D014404:Tubulin / Q000737:chemistry / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D050759:Cyclin-Dependent Kinase Inhibitor p21; D064246:Mad2 Proteins; D010455:Peptides; D014404:Tubulin; D016159:Tumor Suppressor Protein p53; D006160:Guanosine Triphosphate; C584277:BUB1 protein, human; D017346:Protein Serine-Threonine Kinases