title: Lipidomic profiling of sinus mucosa from patients with chronic rhinosinusitis.
abstract: Sinusitis is a cause of significant morbidity, substantial healthcare costs, and negative effects on quality of life. The primary objective of this study is to characterize the previously unknown lipid profile of sinonasal mucosa from patients with chronic rhinosinusitis (CRS) and from controls. Sinus mucosa samples were analyzed from 9 CRS patients with concomitant nasal polyps, 11 CRS patients without polyps, and 12 controls. Ten lone polyp samples were also analyzed. Samples were subjected to a modified Bligh/Dyer lipid extraction, then high performance thin layer chromatography (HPTLC), combined gas chromatography/electron impact-mass spectrometry (GC/EI-MS), and flow-injection/electrospray ionization-tandem mass spectrometry (FI/ESI-MS/MS). Data was analyzed for identification and profiling of major components. HPTLC revealed an array of species reflecting the lipid complexity of the samples. GC/EI-MS revealed cholesterol and several fatty acids. FI/ESI-MSMS revealed numerous lipid species, namely a host of phosphatidylcholines, phosphatidylethanolamines, ceramides and cholesteryl esters, but no detectable amounts of phosphatidyinositols or sulfated lipids. These results are a first step to uncover unique molecular biomarkers in CRS.
authors.affiliation: Pasarow Mass Spectrometry Laboratory, NPI-Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, California, USA; Department of Otorhinolaryngology-Head and Neck Surgery, UCLA Health System, Los Angeles, California, USA; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
authors.forename: Farbod
authors.initials: F
authors.lastname: Fazlollahi
authors.forename: Kessiri
authors.initials: K
authors.lastname: Kongmanas
authors.forename: Nongnuj
authors.initials: N
authors.lastname: Tanphaichitr
authors.forename: Jon
authors.initials: J
authors.lastname: Mallen-St Clair
authors.forename: Quinton
authors.initials: Q
authors.lastname: Gopen
authors.forename: Kym F
authors.initials: KF
authors.lastname: Faull
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Suh
mesh_terms: D015415:Biomarkers / Q000737:chemistry; D016022:Case-Control Studies; D002518:Ceramides / Q000737:chemistry; D002788:Cholesterol Esters / Q000737:chemistry; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D009297:Nasal Mucosa / Q000473:pathology; D009298:Nasal Polyps / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011788:Quality of Life; D012852:Sinusitis / Q000503:physiopathology*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D002518:Ceramides; D002788:Cholesterol Esters; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines
keywords: fatty acids; lipids; metabolism; polyps; tissue