title: In-vitro assessment of the probiotic potential of Lactobacillus plantarum KCC-24 isolated from Italian rye-grass (Lolium multiflorum) forage.
abstract: The aim of the present study was to determine the probiotic potential of the lactic acid bacteria Lactobacillus plantarum KCC-24 (L. plantarum KCC-24), that was isolated and characterized from Italian ryegrass (Lolium multiflorum) forage. The following experiments were performed to assess the probiotic characteristics such as antifungal activity, antibiotic susceptibility, resistance to low pH, stimulated gastric juice and bile salts, proteolytic activity, auto-aggregation, cell surface hydrophobicity, and in vitro antioxidant property. The isolated L. plantarum KCC-24 exhibited significant antifungal activity against the various fungal strains of Aspergillus fumigatus (73.43%), Penicillium chrysogenum (59.04%), Penicillium roqueforti (56.67%), Botrytis elliptica (40.23%), Fusarium oxysporum (52.47%) and it was susceptible to numerous antibiotics, survived in low pH, was resistant to stimulated gastric juices and bile salts (0.3% w/v). Moreover, L. plantarum KCC-24 exhibited good proteolytic activity. In addition L. plantarum KCC-24 showed potent antioxidant and hydrogen peroxide resistant property. In conclusion, the isolated L. plantarum KCC-24 exhibited several characteristics to prove it's excellent as a potential probiotic candidate for developing quality food for ruminant animals and human.
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Mayakrishnan
authors.initials: M
authors.lastname: Vijayakumar
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea.
authors.forename: Soundharrajan
authors.initials: S
authors.lastname: Ilavenil
authors.affiliation: The United Graduate School of Agricultural Sciences, Tottori University, 680-8553 Japan.
authors.forename: Da Hye
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Mariadhas Valan
authors.initials: MV
authors.lastname: Arasu
authors.affiliation: Department of Biochemistry, PSG College of Arts and Science, Affiliated to Bharathiar University, Coimbatore 614-014, Tamil Nadu, India.
authors.forename: Kannappan
authors.initials: K
authors.lastname: Priya
authors.affiliation: Grassland and Forage Division, National Institute of Animal Science, Rural Development Administration, Cheonan 330-801, Republic of Korea. Electronic address: choiwh@korea.kr.
authors.forename: Ki Choon
authors.initials: KC
authors.lastname: Choi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001422:Bacterial Adhesion; D048191:Lactiplantibacillus plantarum / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000502:physiology*; D008129:Lolium / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D012336:RNA, Ribosomal, 16S
keywords: Antifungal; Antioxidant; Auto-aggregation; L. plantarum KCC-24; Probiotics