title: Preparation of squid skin collagen hydrolysate as an antihyaluronidase, antityrosinase, and antioxidant agent.
abstract: A collagen was isolated from squid skin, a processing waste product. The biofunctional activities of enzymatic squid skin collagen hydrolysates were determined to produce a value-added material. Five low-molecular-mass hydrolysate fractions, F1 (>30 kD), F2 (10-30 kD), F3 (3-10 kD), F4 (1-3 kD), and F5 (<1 kD), were manufactured from its enzymatic hydrolysate by ultrafiltration. Fraction F3 had the strongest antihyaluronidase inhibitory activity. Gly, Val, and Pro were major amino acids in F3, while Met, Tyr, and His were minor ones. The molecular mass of F3 was in the range of 3.4 to 10 kD. F3 exhibited copper chelating ability in a concentration-dependent manner. The ferrous chelating ability of F3 was almost 50% at 200 µg/mL. F3 also inhibited tyrosinase activity by 39.65% at 1 mg/mL. Furthermore, F3 had stronger hydroxyl radical scavenging activity (IC50 = 149.94 µg/mL) than ascorbic acid (IC50 = 212.94 µg/mL). Therefore, the squid collagen hydrolysate can be utilized as a nutraceutical or cosmeceutical agent.
authors.affiliation: a Department of Marine Food Science and Technology , Gangneung-Wonju National University , Gangneung , South Korea.
authors.forename: Ladawan
authors.initials: L
authors.lastname: Nakchum
authors.affiliation: a Department of Marine Food Science and Technology , Gangneung-Wonju National University , Gangneung , South Korea.
authors.forename: Sang Moo
authors.initials: SM
authors.lastname: Kim
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D003094:Collagen / Q000737:chemistry* / Q000302:isolation & purification; D049832:Decapodiformes / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D007502:Iron Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D008970:Molecular Weight; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000494:pharmacology*; D012867:Skin / Q000737:chemistry
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D004791:Enzyme Inhibitors; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D011492:Protein Hydrolysates; D003094:Collagen; D014442:Monophenol Monooxygenase; D006821:Hyaluronoglucosaminidase
keywords: Antioxidant; collagen; hyaluronidase; hydrolysate; squid skin; tyrosinase