title: Proteomics reveals the importance of the dynamic redistribution of the subcellular location of proteins in breast cancer cells.
abstract: At the molecular level, living cells are enormously complicated complex adaptive systems in which intertwined genomic, transcriptomic, proteomic and metabolic networks all play a crucial role. At the same time, cells are spatially heterogeneous systems in which subcellular compartmentalization of different functions is ubiquitous and requires efficient cross-compartmental communication. Dynamic redistribution of multitudinous proteins to different subcellular locations in response to cellular functional state is increasingly recognized as a crucial characteristic of cellular function that seems to be at least as important as overall changes in protein abundance. Characterization of the subcellular spatial dynamics of protein distribution is a major challenge for proteomics and recent results with MCF7 breast cancer cells suggest that this may be of particular importance for cancer cells.
authors.affiliation: Division of Medicine, University College London, Centre for Nephrology, Royal Free Campus, Rowland Hill Street, London NW3 2PF, UK.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Pinto
authors.forename: Abdulrab Ahmed M
authors.initials: AA
authors.lastname: Alhaiek
authors.forename: Jasminka
authors.initials: J
authors.lastname: Godovac-Zimmermann
mesh_terms: D006801:Humans; D061986:MCF-7 Cells; D009369:Neoplasms / Q000378:metabolism*; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome
keywords: MCF7 cells; SILAC; breast cancer; cytoplasm; estrogen receptor; mass spectrometry; nucleus; protein trafficking; quantitative proteomics; subcellular protein location