title: Rapid detection of Gram-negative bacteria and their drug resistance genes from positive blood cultures using an automated microarray assay.
abstract: We evaluated the performance of the Verigene Gram-negative blood culture (BC-GN) assay (CE-IVD version) for identification of Gram-negative (GN) bacteria and detection of resistance genes. A total of 163 GN organisms (72 characterized strains and 91 clinical isolates from 86 patients) were tested; among the clinical isolates, 86 (94.5%) isolates were included in the BC-GN panel. For identification, the agreement was 98.6% (146/148, 95% confidence interval [CI], 92.1-100) and 70% (7/10, 95% CI, 53.5-100) for monomicrobial and polymicrobial cultures, respectively. Of the 48 resistance genes harbored by 43 characterized strains, all were correctly detected. Of the 19 clinical isolates harboring resistance genes, 1 CTX-M-producing Escherichia coli isolated in polymicrobial culture was not detected. Overall, BC-GN assay provides acceptable accuracy for rapid identification of Gram-negative bacteria and detection of resistance genes, compared with routine laboratory methods despite that it has limitations in the number of genus/species and resistance gene included in the panel and it shows lower sensitivity in polymicrobial cultures.
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Eunhee
authors.initials: E
authors.lastname: Han
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Dong-Jin
authors.initials: DJ
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Yukyoung
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Jin Kyung
authors.initials: JK
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Kang Gyun
authors.initials: KG
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Seoul St Mary's Hospital, Seoul, Republic of Korea; College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: yjpk@catholic.ac.kr.
authors.forename: Yeon-Joon
authors.initials: YJ
authors.lastname: Park
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D001769:Blood / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D013997:Time Factors
keywords: Automated microarray assay; Polymicrobial; Positive blood cultures; Verigene