title: [Effect of yougui formula granule on ovarian granulosa cells gene expression profiles in IVF patients of shen yang deficiency syndrome].
abstract: OBJECTIVE
  To observe the effect of Yougui Formula Granule (YFG) on ovarian granulosa cells gene expression profiles in in vitro fertilization-embryo transfer (IVF-ET) patients of Shen yang deficiency syndrome (SYDS) from the viewpoint of genomics.
  
  
  METHODS
  Totally 72 infertility patients undergoing IVF-ET were randomly assigned to the treatment group and the control group according to random digit table, 36 in each group. Patients in the treatment group took YFG combined gonadotropin (Gn), while those in the control group took placebos combined Gn. All medication lasted for 3 menstrual cycles before IVF. With high-throughput gene sequencing technology, gene expression profiles of ovarian granulosa cells in the two groups were analyzed to explore the difference by gene ontology (GO) enrichment analysis and kyoto encyclopedia of genes and genomes (KEGG) pathway analysis.
  
  
  RESULTS
  Ovarian granulosa cell gene expression profiles from the follicular fluid showed, when compared with the control group, 391 differential genes were found in the syndrome-control group, 153 down-regulated and 238 upregulated. Enrichment of differentially expressed cellular location and molecular function of genes involved cell proliferation and apoptosis associated cyclin, protein ubiquitination, construction of microtubules and microfilament, mitochondrial function and energy-related factors, regulatory factors for hormone synthesis. Participated pathways involved energy metabolism pathway and transforming growth factor-β (TGF-β) signaling pathway.
  
  
  CONCLUSIONS
  There existed significant difference in gene expression profiles of ovarian granulosa cells between the treatment group and the control group. Differentially expressed genes involved in biological processes correlates with Shen yang deficiency induced proliferation of germ cells, confused apoptosis, and hindered process during which mitochondria produced energy.
authors.affiliation: Integrative Medicine Research Centre of Reproduction and Heredity, Affiliated Hospital of Shangdong University of Traditional Chinese Medicine, Jian, China. f_lian@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Lian
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.forename: Zhen-Gao
authors.initials: ZG
authors.lastname: Sun
authors.forename: Hai-Cui
authors.initials: HC
authors.lastname: Wu
mesh_terms: D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid; D015870:Gene Expression / Q000187:drug effects; D006062:Gonadotropins; D006107:Granulosa Cells; D006801:Humans; D013577:Syndrome; D059467:Transcriptome; D016711:Yang Deficiency
chemical_list: D004365:Drugs, Chinese Herbal; D006062:Gonadotropins; C552041:yougui