title: Aldehyde dehydrogenase 1: a specific cancer stem cell marker for human colorectal carcinoma.
abstract: Since the identification of cancer stem cells (CSCs) a new understanding of tumor occurrence and development has evolved. According to the stem cell (SC) theory, colorectal carcinoma (CRC) SCs may be derived from mutations in normal intestinal cells. CSCs can be defined by their cell of origin (SCs or early progenitor cells). Thus, through a shared stem cell marker between CSCs and SCs, it is possible to investigate the association between its expression and the various clinicopathological features in patients with CRC. Aldehyde dehydrogenase 1 (ALDH1) is an appropriate marker. The present study was performed to examine the role of ALDH1 in CRC. Through indirect fluorescence antibody staining, the association between ALDH1 protein expression and various clinicopathological parameters was investigated. Furthermore, enzyme‑linked immunosorbent assay (ELISA) was used to investigate the differing content of ALDH1 between CRC tissues and normal colorectal tissues. The results revealed that ALDH1 expression was markedly associated with tumor stage, Dukes' stage and the level of tumor cell differentiation. Using ELISA, it was demonstrated that there was a greater level of ALDH1 in CRC tissue than in normal colorectal tissue. Therefore, ALDH1 levels can be used as a useful parameter for pathological evaluation of tissue histology and to predict disease prognosis.
authors.affiliation: Second Department of General Surgery, The First Hospital Qiqihar, Qiqihar, Heilongjiang 161000, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China.
authors.forename: Yin-Dong
authors.initials: YD
authors.lastname: Mu
authors.affiliation: Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China.
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liu
authors.affiliation: Department of Breast Cancer, The First Hospital of Foshan, Foshan, Guangdong 528000, P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Department of Emergency, The First Hospital, Qiqihar, Heilongjiang 161000, P.R. China.
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Ning
authors.affiliation: Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China.
authors.forename: Yue-Zhen
authors.initials: YZ
authors.lastname: Li
authors.affiliation: Life and Technology College Affiliated to Mudanjiang Teachers College, Mudanjiang, Heilongjiang, 157000, P.R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Ding
authors.affiliation: Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang, Heilongjiang 157000, P.R. China.
authors.forename: Ji-Fei
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000080924:Aldehyde Dehydrogenase 1 Family; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D011379:Prognosis; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D047368:Tumor Burden; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; D007527:Isoenzymes; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase