title: Antioxidant components of Viburnum opulus L. determined by on-line HPLC-UV-ABTS radical scavenging and LC-UV-ESI-MS methods.
abstract: Antioxidant activity of the juice and seed and skin extracts prepared with methanol, acetonitrile, and water of Viburnum opulus L. grown in Eastern Black Sea Region were studied with an on-line HPLC-ABTS method and off-line antioxidant methods, among which a linear positive correlation was observed. The fruit extracts were analysed with the HPLC-UV method optimised with 14 standard phenolics. Identification of the phenolic components in the juice was made using an HPLC-UV-ESI-MS method. Nineteen phenolic compounds in juice were identified by comparing the retention times and mass spectra with those of the standards and the phenolics reported in the literature. The major peaks in the juice belonged to coumaroyl-quinic acid, chlorogenic acid, procyanidin B2, and procyanidin trimer. Quite different antioxidant composition profiles were obtained from the extracts with the solvents of different polarities. The antioxidant activities of the seed extracts were higher than those of the skin extracts in general.
authors.affiliation: Giresun University, Espiye Vocational School, Department of Food Processing, 28000 Giresun, Turkey.
authors.forename: Ayça Aktaş
authors.initials: AA
authors.lastname: Karaçelik
authors.affiliation: Faculty of Engineering and Natural Sciences, Gümüşhane University, 29100 Gümüşhane, Turkey; Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey. Electronic address: mkucuk@ktu.edu.tr.
authors.forename: Murat
authors.initials: M
authors.lastname: Küçük
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey.
authors.forename: Zeynep
authors.initials: Z
authors.lastname: İskefiyeli
authors.affiliation: Marmara University, Vocational School of Health Services, Pathology Laboratory Techniques, 34668 İstanbul, Turkey.
authors.forename: Sezgin
authors.initials: S
authors.lastname: Aydemir
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, S4-bis, B-9000 Gent, Belgium.
authors.forename: Seppe
authors.initials: S
authors.lastname: De Smet
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, S4-bis, B-9000 Gent, Belgium.
authors.forename: Bram
authors.initials: B
authors.lastname: Miserez
authors.affiliation: Department of Organic Chemistry, Ghent University, Krijgslaan 281, S4-bis, B-9000 Gent, Belgium.
authors.forename: Patrick
authors.initials: P
authors.lastname: Sandra
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D052160:Benzothiazoles / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016166:Free Radical Scavengers / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis; D013451:Sulfonic Acids / Q000737:chemistry*; D027925:Viburnum / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
keywords: FRAP; Gilaburu; On-line HPLC–ABTS/DPPH; Polyphenols; Snowball; Viburnum opulus