title: Improved and expanded Q-system reagents for genetic manipulations.
abstract: The Q system is a repressible binary expression system for transgenic manipulations in living organisms. Through protein engineering and in vivo functional tests, we report here variants of the Q-system transcriptional activator, including QF2, for driving strong and ubiquitous expression in all Drosophila tissues. Our QF2, Gal4QF and LexAQF chimeric transcriptional activators substantially enrich the toolkit available for transgenic regulation in Drosophila melanogaster.
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Olena
authors.identifier: 0000000200884534
authors.initials: O
authors.lastname: Riabinina
authors.affiliation: 1] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [2] Department of Biology, Stanford University, Stanford, California, USA.
authors.forename: David
authors.initials: D
authors.lastname: Luginbuhl
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Marr
authors.affiliation: Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Mark N
authors.initials: MN
authors.lastname: Wu
authors.affiliation: 1] Howard Hughes Medical Institute, Stanford University, Stanford, California, USA. [2] Department of Biology, Stanford University, Stanford, California, USA.
authors.forename: Liqun
authors.initials: L
authors.lastname: Luo
authors.affiliation: The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Potter
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001522:Behavior, Animal; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian; D005260:Female; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D007814:Larva; D008297:Male; D011401:Promoter Regions, Genetic; D012890:Sleep / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics; D019076:Transgenes
chemical_list: D029721:Drosophila Proteins; C557617:GAL4 protein, Drosophila; D015534:Trans-Activators; D014157:Transcription Factors; D049452:Green Fluorescent Proteins