title: Sepsis progression to multiple organ dysfunction in carotid chemo/baro-denervated rats treated with lipopolysaccharide.
abstract: Sepsis progresses to multiple organ dysfunction (MOD) due to the uncontrolled release of inflammatory mediators. Carotid chemo/baro-receptors could play a protective role during sepsis. In anesthetized male rats, we measured cardiorespiratory variables and plasma TNF-α, glucocorticoids, epinephrine, and MOD marker levels 90min after lipopolysaccharide (LPS) administration in control (SHAM surgery) and bilateral carotid chemo/baro-denervated (BCN) rats. BCN prior to LPS blunted the tachypneic response and enhanced tachycardia and hypotension. BCN-LPS rats also showed blunted plasma glucocorticoid responses, boosted epinephrine and TNF-α responses, and earlier MOD onset with a lower survival time compared with SHAM-LPS rats. Consequently, the complete absence of carotid chemo/baro-sensory function modified the neural, endocrine and inflammatory responses to sepsis. Thus, carotid chemo/baro-receptors play a protective role in sepsis.
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Gino
authors.initials: G
authors.lastname: Nardocci
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Aldo
authors.initials: A
authors.lastname: Martin
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Abarzúa
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Jorge
authors.initials: J
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Fisiopatología Integrativa, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile; Millenium Institute on Immunology and Immunotherapy, Santiago, Chile.
authors.forename: Felipe
authors.initials: F
authors.lastname: Simon
authors.affiliation: Centro de Fisiología Celular e Integrativa, Facultad de Medicina, Clínica Alemana - Universidad del Desarrollo, Santiago, Chile; Dirección de Investigación, Universidad Autónoma de Chile, Santiago, Chile.
authors.forename: Edison P
authors.initials: EP
authors.lastname: Reyes
authors.affiliation: Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Acuña-Castillo
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Cristina
authors.initials: C
authors.lastname: Navarro
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Paula P
authors.initials: PP
authors.lastname: Cortes
authors.affiliation: Laboratorio de Fisiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Biológicas y Facultad de Medicina, Universidad Andrés Bello, Santiago, Chile. Electronic address: rfernandez@unab.cl.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernández
mesh_terms: D000818:Animals; D002344:Carotid Body / Q000187:drug effects / Q000502:physiology*; D003714:Denervation / Q000379:methods; D004837:Epinephrine / Q000097:blood; D005938:Glucocorticoids / Q000097:blood; D006339:Heart Rate / Q000187:drug effects; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009102:Multiple Organ Failure / Q000209:etiology* / Q000378:metabolism; D011311:Pressoreceptors / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012119:Respiration / Q000187:drug effects; D018805:Sepsis / Q000139:chemically induced* / Q000150:complications*; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D013990:Tidal Volume / Q000187:drug effects / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D005938:Glucocorticoids; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D004837:Epinephrine
keywords: Bilateral carotid/sinus neurotomy; Carotid body; Epinephrine; Glucocorticoids; Multiple organ dysfunction; Sepsis; TNF-α; Tissue damage