title: Analysis of the mitochondrial proteome in cytoplasmic male-sterile and male-fertile beets.
abstract: The reported analyses were aimed at identification of the mitochondrial proteome features which were associated with cytoplasmic male sterility (CMS) in beets. The set of analyzed accessions included CMS, maintainer and restored lines. Mitochondrial preparations were subjected to blue-native electrophoresis followed either by in-gel activity assays or separation in denaturing conditions. The CMS condition was associated with decreased activity of complex V and enhancement of additional complexes with the ATPase activity. This was accompanied by accumulation of heptamer HSP60, preSATP6 and an increase in the fraction of the free ATP9 oligomer (not bound to complex V). The ATP9 effect was reversed upon fertility restoration.
  
  
  CONCLUSIONS
  The reported work provides one of very few comprehensive comparisons of the mitochondrial proteomes from cytoplasmic male-sterile (CMS) and male-fertile plants. It shows that in beets the CMS trait is associated with altered functioning of the mitochondrial ATP synthase complex. The presence of CMS-specific ATP synthase subcomplexes resembles deficiencies of this enzyme reported for mammalian cells.
authors.affiliation: Unit of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wesołowski
authors.affiliation: Unit of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland. Electronic address: m.szklarczyk@ur.krakow.pl.
authors.forename: Marek
authors.initials: M
authors.lastname: Szklarczyk
authors.affiliation: Unit of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.
authors.forename: Michał
authors.initials: M
authors.lastname: Szalonek
authors.affiliation: Unit of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland.
authors.forename: Judyta
authors.initials: J
authors.lastname: Słowińska
mesh_terms: D027461:Beta vulgaris / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D051479:Plant Infertility / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*
chemical_list: D024101:Mitochondrial Proteins; D010940:Plant Proteins; D020543:Proteome
keywords: Blue-native electrophoresis; Cytoplasmic male sterility; Mitochondrial proteome