title: Ciglitazone, a peroxisome proliferator-activated receptor gamma ligand, inhibits proliferation and differentiation of th17 cells.
abstract: Peroxisome proliferator-activated receptor gamma (PPARγ) was identified as a cell-intrinsic regulator of Th17 cell differentiation. Th17 cells have been associated with several autoimmune diseases, including experimental autoimmune encephalomyelitis (EAE), inflammatory bowel disease (IBD), and collagen-induced arthritis. In this study, we confirmed PPARγ-mediated inhibition of Th17 cell differentiation and cytokine production at an early stage. Treatment with ciglitazone, a PPARγ ligand, reduced both IL-1β-mediated enhancement of Th17 differentiation and activation of Th17 cells after polarization. For Th17 cell differentiation, we found that ciglitazone-treated cells had a relatively low proliferative activity and produced a lower amount of cytokines, regardless of the presence of IL-1β. The inhibitory activity of ciglitazone might be due to decrease of CCNB1 expression, which regulates the cell cycle in T cells. Hence, we postulate that a pharmaceutical PPARγ activator might be a potent candidate for treatment of Th17-mediated autoimmune disease patients.
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Dong Hyeok
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Ihn
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Chaerin
authors.initials: C
authors.lastname: Moon
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; BK21 Plus, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Sang-Seok
authors.initials: SS
authors.lastname: Oh
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; BK21 Plus, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Soojong
authors.initials: S
authors.lastname: Park
authors.affiliation: College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; BK21 Plus, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Keun Woo
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea; ; PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea;
authors.forename: Kwang Dong
authors.initials: KD
authors.lastname: Kim
keywords: CCNB1; Cell proliferation; IL-17; PPARγ; Th17 cell