title: Application of fluorescent nanoparticles to study remodeling of the endo-lysosomal system by intracellular bacteria.
abstract: Fluorescent nanoparticles (NPs) with desirable chemical, optical and mechanical properties are promising tools to label intracellular organelles. Here, we introduce a method using gold-BSA-rhodamine NPs to label the endo-lysosomal system of eukaryotic cells and monitor manipulations of host cellular pathways by the intracellular pathogen Salmonella enterica. The NPs were readily internalized by HeLa cells and localized in late endosomes/lysosomes. Salmonella infection induced rearrangement of the vesicles and accumulation of NPs in Salmonella-induced membrane structures. We deployed the Imaris software package for quantitative analyses of confocal microscopy images. The number of objects and their size distribution in non-infected cells were distinct from the ones in Salmonella-infected cells, indicating extremely remodeling of the endo-lysosomal system by WT Salmonella.
authors.affiliation: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Krieger
authors.affiliation: Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück; Michael.Hensel@biologie.uni-osnabrueck.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Hensel
mesh_terms: D011992:Endosomes / Q000382:microbiology* / Q000473:pathology; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D008247:Lysosomes / Q000382:microbiology* / Q000473:pathology; D018613:Microscopy, Confocal / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D019779:Salmonella enterica / Q000502:physiology*; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D012710:Serum Albumin, Bovine; D006046:Gold