title: Exploring a new serine protease from Cucumis sativus L.
abstract: Coagulation is an important physiological process in hemostasis which is activated by sequential action of proteases. This study aims to understand the involvement of aqueous fruit extract of Cucumis sativus L. (AqFEC) European burp less variety in blood coagulation cascade. AqFEC hydrolyzed casein in a dose-dependent manner. The presence of protease activity was further confirmed by casein zymography which revealed the possible presence of two high molecular weight protease(s). The proteolytic activity was inhibited only by phenyl methyl sulphonyl fluoride suggesting the presence of serine protease(s). In a dose-dependent manner, AqFEC also hydrolysed Aα and Bβ subunits of fibrinogen, whereas it failed to degrade the γ subunit of fibrinogen even at a concentration as high as 100 μg and incubation time up to 4 h. AqFEC reduced the clotting time of citrated plasma by 87.65%. The protease and fibrinogenolytic activity of AqFEC suggests its possible role in stopping the bleeding and ensuing wound healing process.
authors.affiliation: Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Technical Institutions Campus, Mysore, 570 006, Karnataka, India.
authors.forename: Zohara
authors.initials: Z
authors.lastname: Nafeesa
authors.forename: B R
authors.initials: BR
authors.lastname: Shivalingu
authors.forename: H K
authors.initials: HK
authors.lastname: Vivek
authors.forename: B S
authors.initials: BS
authors.lastname: Priya
authors.forename: S Nanjunda
authors.initials: SN
authors.lastname: Swamy
mesh_terms: D001777:Blood Coagulation / Q000187:drug effects; D003029:Coagulants / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D018553:Cucumis sativus / Q000737:chemistry / Q000201:enzymology*; D005340:Fibrinogen / Q000378:metabolism; D005638:Fruit / Q000737:chemistry / Q000201:enzymology; D006801:Humans; D007700:Kinetics; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D057057:Serine Proteases / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013379:Substrate Specificity
chemical_list: D003029:Coagulants; D010940:Plant Proteins; D005340:Fibrinogen; D057057:Serine Proteases