title: Analysis of Phenolic Compounds in Some Medicinal Herbs by LC-MS.
abstract: In this paper, a liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry in negative mode method was developed for the identification and quantitative determination of 13 individual phenolics (chlorogenic acid, caffeic acid, coumaric acid, ferulic acid, (+)-catechin, (-)-epicatechin, rutin, quercitrin, isoquercitrin, fisetin, isorhamnetin, hesperidin and chrysin) from ethanolic extracts [30, 50 and 70% (w/v)] of Calendula officinalis, Hypericum perforatum, Galium verum and Origanum vulgare and some commercial extracts of these medicinal herbs. Correlation coefficients (r(2)) from calibration curves for all the compounds were between 0.9971 and 0.9996. Limit of detection was in the range of 0.070-0.280 µg/mL and limit of quantification was from 0.233 to 0.932 µg/mL. The method was partially validated and the results obtained are: the intra- and interday relative standard deviation values were within 0.086 and 2.821% and recovery values vary from 95.84% (coumaric acid) to 103.20% (rutin).
authors.affiliation: Centre of Bioanalysis, National Institute for Biological Science, Bucharest, Romania.
authors.forename: Alina O
authors.initials: AO
authors.lastname: Matei
authors.affiliation: Centre of Bioanalysis, National Institute for Biological Science, Bucharest, Romania.
authors.forename: Florentina
authors.initials: F
authors.lastname: Gatea
authors.affiliation: Centre of Bioanalysis, National Institute for Biological Science, Bucharest, Romania Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania rglucian2000@yahoo.com.
authors.forename: Gabriel L
authors.initials: GL
authors.lastname: Radu
mesh_terms: D020838:Calendula / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032061:Galium / Q000737:chemistry*; D020902:Hypericum / Q000737:chemistry*; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D031341:Origanum / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry
chemical_list: D010636:Phenols; D010936:Plant Extracts