title: Yogurt Containing the Probacteria Lactobacillus acidophilus Combined with Natural Antioxidants Mitigates Doxorubicin-Induced Cardiomyopathy in Rats.
abstract: Probiotics and antioxidants have a definite improving effect in cardiovascular diseases. This study aims at mitigating doxorubicin toxicity on cardiac function through consuming a functional food. Five groups of adult male Sprague-Dawley rats were used along 22 weeks. Group I received 30 g/kg/day food enriched with yogurt, green tea extract, and carrots (80, 0.84, and 100 g/kg diet, respectively) from the first week, group II received carvedilol 30 mg/kg/day orally from week 17, group III received both carvedilol and tested food, and groups IV and V were +ve and -ve control groups, respectively. In week 17, cardiomyopathy was induced by i.p. injection of 2.5 mg/kg doxorubicin every 48 h for 2 weeks. Histopathological and electrophysiological examinations and biochemical analysis were done. Lipid peroxidation, antioxidant effect, heart failure compensatory mediators, and proinflammatory cytokines were assessed. Tested food normalized time between the start of Q wave and the end of T wave on electrocardiogram (QT interval) and heart rate compared to the doxorubicin group (P<.05). It also improved hypertrophy indicated by a significant (P<.05) decrease in heart/body weight ratio, angiotensin-II (Ang-II), and atrial natriuretic peptide (ANP) serum levels. Histopathological examination of cardiac sections from the tested food group revealed less marked vacuolization and low perivascular fibrosis percentage (0.7803 ± 0.04). A significant (P<.001) decrease in serum creatine kinase-membrane bound, lactate dehydrogenase, triglycerides, cholesterol, low-density lipoprotein cholesterol, and tissue malondialdehyde (MDA) levels was observed in addition to an increase in serum Na(+)/K(+) ATP1A1 and cardiac reduced glutathione (GSH) levels. Tested food also lowered the inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) serum levels significantly (P<.01). Probiotic food containing Lactobacillus acidophilus, green tea, and carrots can improve membrane integrity and cardiac contractility in doxorubicin-induced cardiomyopathy by decreasing TNF-α, IL-6, MDA, increasing GSH, and modulating compensatory mediators such as Ang-II and ANP.
authors.affiliation: 1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University , Mansoura, Egypt .
authors.forename: Nashwa M
authors.initials: NM
authors.lastname: Abu-Elsaad
authors.affiliation: 1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University , Mansoura, Egypt .
authors.forename: Ahmed G
authors.initials: AG
authors.lastname: Abd Elhameed
authors.affiliation: 2 Department of Pathology, Faculty of Medicine, Mansoura University , Mansoura, Egypt .
authors.forename: Amr
authors.initials: A
authors.lastname: El-Karef
authors.affiliation: 1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University , Mansoura, Egypt .
authors.forename: Tarek M
authors.initials: TM
authors.lastname: Ibrahim
mesh_terms: D000804:Angiotensin II / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use*; D009320:Atrial Natriuretic Factor / Q000097:blood; D028241:Camellia sinensis / Q000737:chemistry; D002227:Carbazoles / Q000494:pharmacology; D009202:Cardiomyopathies / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D002316:Cardiotonic Agents / Q000627:therapeutic use; D000077261:Carvedilol; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000097:blood; D018552:Daucus carota / Q000737:chemistry; D004317:Doxorubicin / Q000009:adverse effects; D055951:Functional Food; D006321:Heart / Q000187:drug effects* / Q000503:physiopathology; D007779:Lactobacillus acidophilus; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D019936:Probiotics / Q000627:therapeutic use; D011412:Propanolamines / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D015014:Yogurt / Q000382:microbiology*
chemical_list: D000975:Antioxidants; D002227:Carbazoles; D002316:Cardiotonic Agents; D016207:Cytokines; D011412:Propanolamines; D000077261:Carvedilol; D000804:Angiotensin II; D008315:Malondialdehyde; D004317:Doxorubicin; D009320:Atrial Natriuretic Factor; D002784:Cholesterol
keywords: IL; TNF; cardiomyopathy; doxorubicin; functional food; probiotics