title: Antiepileptic C21 steroids from the roots of Cynanchum otophyllum.
abstract: In order to discover more natural products possessing potentially antiepileptic activities, three C21 steroids, including a new one, characterized as caudatin-3-O-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside (1), and two known analogs, otophylloside B (2) and caudatin-3-O-β-D-oleandropyranosyl-(1 → 4)-β-D-oleandropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside (3), were isolated from the chloroform extract of the roots of Cynanchum otophyllum and evaluated for their antiepileptic activities by pentylenetrazole (PTZ)-induced zebrafish larval locomotor assay. The results showed that all of them had marked activities of suppressing PTZ-induced seizure behaviors in larval zebrafish at the dose of 10 μg/ml.
authors.affiliation: a Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming 650500 , China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Mi
authors.initials: M
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
authors.forename: Bao-Cai
authors.initials: BC
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031105:Cynanchum / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D018517:Plant Roots / Q000737:chemistry; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015027:Zebrafish
chemical_list: D000927:Anticonvulsants; D006027:Glycosides; D013256:Steroids; C000602163:caudatin-3-O-beta-D-cymaropyranosyl-(1-4)-beta-D-cymaropyranosyl-(1-4)-beta-D-cymaropyranoside
keywords: C21 steroid; Cynanchum otophyllum; antiepileptic effect; zebrafish