title: HPTLC analysis of Scoparia dulcis Linn (Scrophulariaceae) and its larvicidal potential against dengue vector Aedes aegypti.
abstract: This study evaluates the larvicidal activity of Scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. The extract was tested at various concentrations ranging from 0.1 to 2 mg/mL against Aedes aegypti larvae. The extracts displayed significant larvicidal efficacy against Ae. aegypt species after 24 h exposure revealing LC50 of 3.3835 (mg/mL) and LC90 of 5.7578 (mg/mL). Finger printing profile carried out by CAMAG automatic TLC sample applicator programmed through WIN CATS software revealed peaks with different Rf values for three different volumes injected: 16, 15 and 18 peaks were spotted for 3, 6 and 9 μL, respectively. Ascending order of Rf values was also ascertained for each peak recorded. This study clearly signifies that S. dulcis extract contains numerous compounds that are known to have larvicidal properties which clearly substantiates its efficacy on Ae. aegypti larvae.
authors.affiliation: a Department of Physiology , Dr. ALM PG Institute of Basic Medical Sciences, University of Madras, Taramani Campus , Chennai-600 113 , Tamilnadu , India.
authors.forename: Wankupar
authors.initials: W
authors.lastname: Wankhar
authors.forename: Sakthivel
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Sheeladevi
authors.initials: S
authors.lastname: Rathinasamy
mesh_terms: D000330:Aedes; D000818:Animals; D007306:Insecticides / Q000737:chemistry*; D007814:Larva; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D036721:Scoparia / Q000737:chemistry*
chemical_list: D007306:Insecticides; D010936:Plant Extracts
keywords: Aedes aegypti; HPTLC; Scoparia dulcis; dengue; larvicidal