title: Assessment of T-2 toxin effect and its metabolite HT-2 toxin combined with insulin-like growth factor I, leptin and ghrelin on progesterone secretion by rabbit ovarian fragments.
abstract: Assessment of A-trichothecene mycotoxins (T-2 and HT-2 toxins) effect combined with growth factor IGF-I, and the metabolic hormones leptin and ghrelin on progesterone secretion by rabbit ovarian fragments was studied. Rabbit ovarian fragments were incubated without (control group) or with T-2/HT-2 toxin, or their combinations with insulin-like growth factor I (IGF-I), leptin or ghrelin at various concentrations for 24 h. Secretion of progesterone was determined by ELISA. First, T-2 toxin and HT-2 toxins at all doses used (0.01, 0.1, 1, 10, and 100 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Second, T-2 toxin but not HT-2 toxin combined with IGF-I was shown to be potential regulator of progesterone secretion in rabbit ovarian fragments. T-2 toxin at all doses used (0.01; 0.1; 1; 10; and 100 ng mL(-1)) combined with IGF-I (at dose 100 ng mL(-1)) significantly (P < 0.05) decreased progesterone secretion by rabbit ovarian fragments. Third, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL(-1)) combined with leptin (at dose 1000 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Furthermore, T-2 toxin and HT-2 toxin at all doses used in the study (0.01, 0.1, 1, 10, and 100 ng mL(-1)) combined with ghrelin (500 ng mL(-1)) were not shown to be potential regulators of progesterone secretion in rabbit ovarian fragments. Results in this study showed that trichothecene as T-2 toxin combined with IGF-I but not HT-2 toxin was able to decrease progesterone secretion in rabbit ovarian fragments in vitro. Experimental results of T-2 and HT-2 toxins combined with leptin and ghrelin did not confirm ability to modulate progesterone secretion by ovarian fragments in rabbits.
authors.affiliation: a Department of Animal Physiology, Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.
authors.forename: Nora
authors.initials: N
authors.lastname: Maruniakova
authors.forename: Attila
authors.initials: A
authors.lastname: Kadasi
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Sirotkin
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Leśniak
authors.forename: Ana M M
authors.initials: AM
authors.lastname: Ferreira
authors.forename: Jozef
authors.initials: J
authors.lastname: Bulla
authors.forename: Adriana
authors.initials: A
authors.lastname: Kolesarova
mesh_terms: D000818:Animals; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D054439:Ghrelin / Q000494:pharmacology*; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000494:pharmacology*; D020738:Leptin / Q000494:pharmacology*; D009183:Mycotoxins / Q000633:toxicity; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D011817:Rabbits; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000633:toxicity*
chemical_list: D004338:Drug Combinations; D054439:Ghrelin; D018970:Insulin-Like Growth Factor Binding Protein 1; D020738:Leptin; D009183:Mycotoxins; D011374:Progesterone; D013605:T-2 Toxin; C012351:HT-2 toxin
keywords: HT-2 toxin; IGF-I; Progesterone; T-2 toxin; ghrelin; leptin; ovary; rabbit