title: [Purification of monoclonal antibody to clenbuterol and its biology identity].
abstract: OBJECTIVE
  To identify the self-preparation monoclonal antibody which target to clenbuterol, and set up the standard curve to clenbuterol (CL) detection.
  
  
  METHODS
  The affinity constants and activity of the monoclonal antibody which target to CL were determined by ELISA. ELISA was also used to confirm whether the monoclonal antibody had any across-reaction with BSA and CL analogues. The rat ascites which contains the monoclonal antibody target to CL was purified by (NH4)2SO4 salt-out method and further by affinity column. At last, the CL detection standard curve which based on indirect competition ELISA was established.
  
  
  RESULTS
  The ELISA experiment showed that the antibody titer was 10(6) and the monoclonal antibody affinity constants was 2.90 x 10(10) L/mol. The result of the indirect competition ELISA confirmed that the monoclonal antibody had no cross-reaction with BSA and a few kind of CL analogue. CL detection standard curve based on indirect competition ELISA was established, which R2 was 0.9812, and the lowest detectable limit was 1.0 ng/ml.
  
  
  CONCLUSIONS
  The standard curve based on indirectly competitioning ELISA was established. The self-preparation monoclonal antibody which target to CL has high affinity and high specific to CL, which had established the foundation to the advanced development of the CL immune test paper and CL ELISA kit.
authors.affiliation: Academy of Military Medical Sciences, Tianjin, China.
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Li
authors.forename: Bao-an
authors.initials: BA
authors.lastname: Ning
authors.forename: Nan
authors.initials: N
authors.lastname: Liu
authors.forename: Xin-hua
authors.initials: XH
authors.lastname: Ma
authors.forename: Guo-rong
authors.initials: GR
authors.lastname: Ou
authors.forename: Zhi-xian
authors.initials: ZX
authors.lastname: Gao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000302:isolation & purification*; D000915:Antibody Affinity; D002976:Clenbuterol / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D057230:Limit of Detection; D051381:Rats
chemical_list: D000911:Antibodies, Monoclonal; D002976:Clenbuterol