title: Biomechanical properties of hybrid heart valve prosthesis utilizing the pigs that do not express the galactose-α-1,3-galactose (α-Gal) antigen derived tissue and tissue engineering technique.
abstract: The aim of the study was to estimate the biomechanical properties of heart valves conduit derived from transgenic pigs to determine the usefulness for the preparation of tissue-engineered heart valves. The acellular aortic and pulmonary valve conduits from transgenic pigs were used to estimate the biomechanical properties of the valve. Non-transgenic porcine heart valve conduits were used as a reference. The biomechanics stability of acellular valve conduits decreased both for the transgenic and non-transgenic porcine valves. The energy required to break the native pulmonary valve derived from transgenic pigs was higher (20,475 ± 7,600 J m(-2)) compared with native non-transgenic pigs (12,140 ± 5,370 J m(-2)). After acellularization, the energy to break the valves decreased to 14,600 and 8,800 J m(-2) for the transgenic pulmonary valve and non-transgenic valve, respectively. The native transgenic pulmonary valve showed a higher extensibility (42.70 %) than the non-transgenic pulmonary valve (35.50 %); the extensibility decreased after acellularization to 41.1 and 31.5 % for the transgenic and non-transgenic valves, respectively. The pulmonary valves derived from transgenic pigs demonstrate better biomechanical properties compared with non-transgenic. Heart valves derived from transgenic pigs can be valuable for the preparation of tissue-engineered bioprostheses, because of their biomechanical properties, stability, reduced immune response, making them safer for clinical applications.
authors.affiliation: Bioengineering Laboratory, Heart Prosthesis Institute, Wolnosci 345a, 41-800, Zabrze, Poland, mildes@post.pl.
authors.forename: Piotr
authors.initials: P
authors.lastname: Wilczek
authors.forename: Anna
authors.initials: A
authors.lastname: Lesiak
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Niemiec-Cyganek
authors.forename: Barbara
authors.initials: B
authors.lastname: Kubin
authors.forename: Ryszard
authors.initials: R
authors.lastname: Slomski
authors.forename: Jerzy
authors.initials: J
authors.lastname: Nozynski
authors.forename: Grazyna
authors.initials: G
authors.lastname: Wilczek
authors.forename: Aldona
authors.initials: A
authors.lastname: Mzyk
authors.forename: Michalina
authors.initials: M
authors.lastname: Gramatyka
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D000941:Antigens / Q000276:immunology*; D001696:Biomechanical Phenomena; D004187:Disaccharides / Q000276:immunology*; D006350:Heart Valve Prosthesis; D013552:Swine; D023822:Tissue Engineering
chemical_list: D000941:Antigens; D004187:Disaccharides; C055075:galactosyl-(1-3)galactose