title: The role of eicosanoids in 17α, 20β-dihydroxy-4-pregnen-3-one-induced ovulation and spawning in Danio rerio.
abstract: This study employed a hormone bioassay to characterize the eicosanoids involved in zebrafish ovulation and spawning, in particular the prostaglandin (PG) products of cyclooxygenase (COX) metabolism and the leukotriene (LT) products of lipoxygenase (LOX) metabolism. Exposure to the teleost progestogen 17α, 20β-dihydroxy-4-pregnen-3-one (17,20βP) induced ovulation, but not spawning, in solitary females and both ovulation and spawning in male-female pairs. Transcription of the eicosanoid-synthesizing enzymes cytosolic phospholipase A2 (cPLA(2)) and COX-2 increased and LTC(4) synthase decreased in peri-ovulatory ovaries of 17,20βP-exposed fish. Ovarian PGF(2α) levels increased post-spawning in 17,20βP-exposed fish, but there was no difference in LTB(4) or LTC(4). Pre-exposure to cPLA(2) or LOX inhibitors reduced 17,20βP-induced ovulation rates, while a COX inhibitor had no effect on ovulation or spawning. Collectively, these findings suggest that eicosanoids, in particular LOX metabolites, mediate 17,20βP-induced ovulation in zebrafish. COX metabolites also appear to be involved in ovulation and spawning but their role remains undefined.
authors.affiliation: Department of Integrative Biology, University of Guelph, Guelph, ON, Canada.
authors.forename: Olivia M
authors.initials: OM
authors.lastname: Knight
authors.affiliation: Department of Integrative Biology, University of Guelph, Guelph, ON, Canada. Electronic address: gvanderk@uoguelph.ca.
authors.forename: Glen
authors.initials: G
authors.lastname: Van Der Kraak
mesh_terms: D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics; D006908:Hydroxyprogesterones / Q000494:pharmacology*; D008297:Male; D010053:Ovary / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D054467:Phospholipases A2 / Q000235:genetics; D011372:Progestins / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012098:Reproduction / Q000187:drug effects* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D006908:Hydroxyprogesterones; D011372:Progestins; D011453:Prostaglandins; D012333:RNA, Messenger; C007433:17,20-dihydroxy-4-pregnen-3-one; D051546:Cyclooxygenase 2; D005982:Glutathione Transferase; D054467:Phospholipases A2; C041116:leukotriene-C4 synthase
keywords: Arachidonic acid; Leukotriene; Maturation-inducing steroid; Prostaglandin; Reproduction; Zebrafish