title: Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases.
abstract: We describe a nucleic acid testing (NAT) platform for infectious disease diagnostics at the point-of-care, using surface acoustic waves (SAW) to perform a multiplexed loop-mediated isothermal amplification (LAMP) test for sexually transmitted diseases. The ultrasonic actuation not only enables faster NAT reactions but also provides a route towards integrating low-cost, low-power molecular diagnostics into disposable sensors.
authors.affiliation: Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK. julien.reboud@glasgow.ac.uk.
authors.forename: Gaolian
authors.initials: G
authors.lastname: Xu
authors.forename: Rory N
authors.initials: RN
authors.lastname: Gunson
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Cooper
authors.forename: Julien
authors.initials: J
authors.lastname: Reboud
mesh_terms: D002692:Chlamydia trachomatis / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D009691:Nucleic Acid Denaturation; D019095:Point-of-Care Systems; D012749:Sexually Transmitted Diseases / Q000175:diagnosis* / Q000382:microbiology*; D013696:Temperature; D014465:Ultrasonics
chemical_list: D004269:DNA, Bacterial