title: Protective effects of vitamin E and Cornus mas fruit extract on methotrexate-induced cytotoxicity in sperms of adult mice.
abstract: This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE) and vitamin E (Vit E) on sperm quality parameters in the methotrexate (MTX)-treated mice. Forty-eight young adult male mice (8-12 weeks) were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (20 mg kg(-1), ip, once weekly), MTX + CMFE (250 mg kg(-1)), MTX + CMFE (500 mg kg(-1)), MTX + CMFE (1000 mg kg(-1)), and MTX + Vit E (100 IU kg(-1), po) for 35 consecutive days. On day 35, after euthanasia the epididymal sperms were isolated. Then the total mean sperm count, sperm viability and motility were determined. The total antioxidant capacity (TAOC) of all experimental groups were also evaluated. The MTX-treated animals showed a significant changes in all parameters of sperm quality assessment compared to the control group. Both Vit E and CMFE were able to protect from MTX-induced effects on sperm maturity and DNA damage. Co-administration of MTX and CMFE and/or Vit E resulted in protection from MTX-reduced TAOC. In conclusion, these data suggested that MTX administration could adversely affect the sperm quality. Moreover, the protective effect of Vit E and CMFE on MTX-induced sperm toxicity was also documented.
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Leila
authors.initials: L
authors.lastname: Zarei
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Rajabali
authors.initials: R
authors.lastname: Sadrkhanlou
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Rasoul
authors.initials: R
authors.lastname: Shahrooz
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Hassan
authors.initials: H
authors.lastname: Malekinejad
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Medical Sciences, Urmia, Iran.
authors.forename: Behroz
authors.initials: B
authors.lastname: Eilkhanizadeh
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Abbas
authors.initials: A
authors.lastname: Ahmadi
keywords: Cornus mas; Methotrexate; Mouse; Sperm quality; Vitamin E