title: Methods and compositions for amplification and detection of microRNAs (miRNAs) and noncoding RNAs (ncRNAs) using the signature sequence amplification method (SSAM).
abstract: The signature sequence amplification method (SSAM) described herein is an approach for amplifying noncoding RNA (ncRNA), microRNA (miRNA), and small polynucleotide sequences. A key point of the SSAM technology is the generation of signature sequences. The signature sequences include target sequences (miRNA, ncRNA, and/or any small polynucleotide sequence) flanked by two DNA fragments. Target sequences can be amplified through DNA synthesis, RNA synthesis, or the combination of DNA and RNA synthesis. The amplification of signature sequences provides an efficient and reproducible mechanism to determine the presence or absence of the target miRNAs/ncRNAs, to analyze the quantities of the miRNAs in biological samples, and for miRNA/ncRNA profiling.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Ginsberg
authors.affiliation: Center for Dementia Research, Nathan Kline Institute, NYU Langone Medical Center, 140 Old Orangeburg Road, Orangeburg, NY 10962, 845-398-2170, USA. ginsberg@nki.rfmh.org.
authors.forename: Shaoli
authors.initials: S
authors.lastname: Che
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D022661:RNA, Untranslated / Q000032:analysis* / Q000737:chemistry
chemical_list: D035683:MicroRNAs; D022661:RNA, Untranslated