title: Enhanced lipid production with undetoxified corncob hydrolysate by Rhodotorula glutinis using a high cell density culture strategy.
abstract: In recent years, energy crisis and environmental issues such as greenhouse effect, global warming, etc. has roused peoples' concern. Biodiesel, as renewable energy, has attracted much attention to deal with such problems. This work studied the lipid production by Rhodotorula glutinis with undetoxified corncob hydrolysate. The results indicated that R. glutinis had high tolerance to the inhibitors in corncob hydrolysate and it could utilize undetoxified corncob hydrolysate directly for lipid production. The cell grew well with undetoxified hydrolysate in the batch culture of 5L fermentor with the optimized C/N ratio of 75, lipid titer and lipid content reached 5.5g/L and 36.4%, respectively. High cell density culture with two-stage nitrogen feeding strategy was studied to enhance the lipid production, biomass, lipid concentration and lipid content of 70.8, 33.5g/L and 47.2% were obtained. The results indicated the potential application for lipid production by R. glutinis with corncob hydrolysate directly.
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China; Department of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China. Electronic address: liuhongjuan@tsinghua.edu.cn.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China. Electronic address: zhangja@tsinghua.edu.cn.
authors.forename: Jian'an
authors.initials: J
authors.lastname: Zhang
mesh_terms: D019342:Acetic Acid / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism; D005662:Furaldehyde / Q000378:metabolism; D006868:Hydrolysis; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000737:chemistry; D009584:Nitrogen / Q000378:metabolism; D012248:Rhodotorula / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008055:Lipids; D002244:Carbon; D014994:Xylose; D005662:Furaldehyde; D009584:Nitrogen; D019342:Acetic Acid
keywords: Biofuel; Fed-batch culture; Lipid; Rhodotorula glutinis; Undetoxified hydrolysate