title: Genomic and metabolic analysis of fluoranthene degradation pathway in Celeribacter indicus P73T.
abstract: Celeribacter indicus P73(T), isolated from deep-sea sediment from the Indian Ocean, is capable of degrading a wide range of polycyclic aromatic hydrocarbons (PAHs) and is the first fluoranthene-degrading bacterium within the family Rhodobacteraceae. Here, the complete genome sequence of strain P73(T) is presented and analyzed. Besides a 4.5-Mb circular chromosome, strain P73(T) carries five plasmids, and encodes 4827 predicted protein-coding sequences. One hundred and thirty-eight genes, including 14 dioxygenase genes, were predicted to be involved in the degradation of aromatic compounds, and most of these genes are clustered in four regions. P73_0346 is the first fluoranthene 7,8-dioxygenase to be discovered and the first fluoranthene dioxygenase within the toluene/biphenyl family. The degradative genes in regions B and D in P73(T) are absent in Celeribacter baekdonensis B30, which cannot degrade PAHs. Four intermediate metabolites [acenaphthylene-1(2H)-one, acenaphthenequinone, 1,2-dihydroxyacenaphthylene, and 1,8-naphthalic anhydride] of fluoranthene degradation by strain P73(T) were detected as the main intermediates, indicating that the degradation of fluoranthene in P73(T) was initiated by dioxygenation at the C-7,8 positions. Based on the genomic and metabolitic results, we propose a C-7,8 dioxygenation pathway in which fluoranthene is mineralized to TCA cycle intermediates.
authors.affiliation: 1] State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China [2] School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Cao
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D001682:Biological Availability; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D049308:Dioxygenases; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D016680:Genome, Bacterial; D023281:Genomics; D055432:Metabolomics; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D010957:Plasmids / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D020543:Proteome / Q000378:metabolism
chemical_list: D004270:DNA, Circular; D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D020543:Proteome; C007738:fluoranthene; D049308:Dioxygenases