title: Neuregulin 1 affects leptin levels, food intake and weight gain in normal-weight, but not obese, db/db mice.
abstract: OBJECTIVE
  Studies in vitro have highlighted the potential involvement of neuregulin 1 (NRG1) in the regulation of energy metabolism. This effect has also been suggested in vivo, as intracerebroventricular injection of NRG1 reduces food intakes and weight gain in rodents. Thus, it was hypothesised that NRG1 might affect serum leptin levels in mice.
  
  
  METHODS
  Weight, food intakes, energy expenditure, spontaneous physical activity and serum leptin levels were evaluated in normal-weight C57BL/6JRJ mice following intraperitoneal administration of NRG1 (50 μg/kg, three times/week) or saline for 8 weeks. Based on the results of this first experiment, leptin-resistant obese db/db mice were then given NRG1 for 8 weeks.
  
  
  RESULTS
  Leptin serum concentrations were six times higher in C57BL/6JRJ mice treated with NRG1 than in the animals given saline. NRG1 treatment also reduced weight gain by 10% and food intakes by 15% compared with saline treatment, while energy expenditure remained unchanged. In db/db mice, serum leptin concentrations, weight gain, food intakes, energy expenditure and spontaneous physical activity were not altered by NRG1 treatment.
  
  
  CONCLUSIONS
  The decrease in food intakes and weight gain associated with NRG1 treatment in C57BL/6JRJ mice may be partly explained by increased leptin levels, whereas db/db mice were not affected by the treatment, suggesting resistance to NRG1 in this pathological state.
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France.
authors.forename: G
authors.initials: G
authors.lastname: Ennequin
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France.
authors.forename: N
authors.initials: N
authors.lastname: Boisseau
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France.
authors.forename: K
authors.initials: K
authors.lastname: Caillaud
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France.
authors.forename: V
authors.initials: V
authors.lastname: Chavanelle
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France.
authors.forename: M
authors.initials: M
authors.lastname: Etienne
authors.affiliation: Zensun Sci and Tech Ltd., Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.affiliation: Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France; French National Institute for Agricultural Research (INRA), UMR 1019, UNH, CRNH Auvergne, 63000 Clermont-Ferrand, France; UFR Médecine, University Clermont 1, 63001 Clermont-Ferrand, France.
authors.forename: C
authors.initials: C
authors.lastname: Montaurier
authors.affiliation: EA 3533, Laboratory of Metabolic Adaptations to Exercise in Physiological and Pathological Conditions, Clermont University, Blaise Pascal University, BP 10448, 63000 Clermont-Ferrand, France; Clermont University, Auvergne University, Human Nutrition Unit, BP 10448, 63000 Clermont-Ferrand cedex 1, France. Electronic address: pascal.sirvent@univ-bpclermont.fr.
authors.forename: P
authors.initials: P
authors.lastname: Sirvent
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D004435:Eating / Q000187:drug effects*; D004734:Energy Metabolism / Q000187:drug effects; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood*; D051379:Mice; D008820:Mice, Obese; D020890:Neuregulin-1 / Q000494:pharmacology*; D015430:Weight Gain / Q000187:drug effects*
chemical_list: D007328:Insulin; D020738:Leptin; D020890:Neuregulin-1
keywords: Energy expenditure; NRG1; Obesity; Weight loss