title: [Study on the epidemiologic characteristic of Brucella melitensis isolated in Guizhou province in 2010-2012].
abstract: OBJECTIVE
  To understand the genetic and epidemiologic characteristic of Brucella (B.) melitensis strains isolated in Guizhou province in 2010-2012.
  
  
  METHODS
  B. genus specific BCSP31-PCR and species-specific AMOS-PCR were used to identify the bacteria strain, while the identified strains were analyzed under MLVA-16 and cluster analysis of B. melitensis strains. The strains were isolated from Guizhou and other provinces.
  
  
  RESULTS
  Six B. melitensis strains were identified as B. melitensis using the BCSP31-PCR and AMOS-PCR. Data from the MLVA-16 analysis revealed the differences of repeated numbers at parts of the VNTR locus in the six strains isolated in Guizhou province. The six strains from Guizhou province and 105 B. melitensis strains from other province could be divided into 72 MLVA types(MT). Strain ZY and ZA from Guizhou province were typed as MT63, and LL3, LL4 and LL11 were typed as MT67, while strain SQ was typed as MT72. Data from the clustering analysis showed that ZY,ZA, LL3, LL4 and LL9 were most closely clustered with B. melitensis isolates from Yunnan, Fujian and Guangdong provinces, but strain SQ was genetically remote from other strains.
  
  
  CONCLUSIONS
  PCR methods, combined with MLVA-16, identified the six B. melitensis strains isolated in Guizhou province in 2010-2012 as B. melitensis biovar 3, with the genetic diversity of the strains showed. Six strains were closely related to the B. melitensis strains from Yunnan, Fujian and Guangdong provinces. The results of this study provided scientific basis for the control and prevention of Brucellosis in Guizhou province.
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China. Email: zjumedjun@163.com.
authors.forename: Shijun
authors.initials: S
authors.lastname: Li
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Jingzhu
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Yu
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Tian
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Zhiting
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Guangpeng
authors.initials: G
authors.lastname: Tang
authors.affiliation: Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004, China.
authors.forename: Dingming
authors.initials: D
authors.lastname: Wang
mesh_terms: D017347:Brucella melitensis / Q000235:genetics* / Q000302:isolation & purification*; D002006:Brucellosis / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D014644:Genetic Variation; D006801:Humans; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction