title: Ameliorative properties of aqueous extract of Ficus thonningii on erythrocyte osmotic fragility induced by acetaminophen in Rattus norvegicus.
abstract: In vitro antioxidant and erythrocyte protecting activities by aqueous extract of Ficus thonningii leaves on blood cells were studied in acetaminophen treated rats. The extract was safe at limit dose of 5000 mg kg(-1) body weight. The extract demonstrated dose dependent antihemolytic effect at dose levels between 50 and 200 mg kg(-1) body weight. The lowest antihemolytic effect was observed at dose level of 200 mg kg(-1) body given the lowest percentage hemolysis of 10.53 ± 1.76%, whereas the highest percentage hemolysis at dose level of 50 mg kg(-1) was 29.02 ± 7.45%. Hematology revealed erythrocytosis at dose levels of 100 and 200 mg kg(-1) body weight. Hyperglobinemia and lymphocytopenia were observed at dose levels of 100 mg kg(-1) and 200 mg kg(-1), respectively. The extract effectively showed scavenging activity on a stable oxidative radical diphenylpicrylhydrazyl (DPPH) and a significant ferric reducing antioxidant power (FRAP) activity. The plausible erythrocyte membrane protective effect may be due to its free radical scavenging activity and hence the extract can be used to improve hematological parameters and ameliorate oxidative stress.
authors.affiliation: Department of Veterinary Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Agriculture, Makurdi, Nigeria;
authors.forename: Victor Masekaven
authors.initials: VM
authors.lastname: Ahur
authors.affiliation: Department of Veterinary Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Agriculture, Makurdi, Nigeria;
authors.forename: Yahaya Adenkola
authors.initials: YA
authors.lastname: Adenkola
authors.affiliation: Department of Veterinary Physiology, Pharmacology and Biochemistry, College of Veterinary Medicine, University of Agriculture, Makurdi, Nigeria;
authors.forename: Saganuwan Alhaji
authors.initials: SA
authors.lastname: Saganuwan
authors.affiliation: Division of Livestock Services, Benue State Ministry of Agriculture and Natural Resources, Makurdi, Nigeria.
authors.forename: Job Terungwa
authors.initials: JT
authors.lastname: Ikye-Tor
keywords: Acetaminophen; Erythrocytes; Ficus thonningii; Osmotic fragility