title: Effect of small nuclear ribonucleoprotein-associated polypeptide N on the proliferation of medulloblastoma cells.
abstract: Spliceosome mutations have been reported in various types of cancer and a number of antitumor drugs have been observed to tightly bind to spliceosome components. Small nuclear ribonucleoprotein‑associated polypeptide N (SNRPN) is a small ribonuclear protein and is a key spliceosome constituent. However, the role of SNRPN in human medulloblastoma remains unknown. In the present study, the effect of SNRPN on cell growth was investigated in vitro using the Daoy human medulloblastoma cell line. Lentivirus (Lv)-mediated short hairpin (sh) RNA was used to silence SNRPN expression, which was verified by reverse transcription‑quantitative polymerase chain reaction and western blotting. Cell proliferation was examined by MTT and colony formation assays. Knockdown of SNRPN markedly reduced the proliferation and colony formation ability of Daoy medulloblastoma cells. In addition, flow cytometric analysis revealed that the cell cycle distribution was altered when the Daoy cells were infected with Lv‑shSNRPN. To the best of our knowledge, this is the first study to investigate the effect of SNRPN on cell proliferation in medulloblastoma. The results indicate that SNRPN may be a potential novel target for the development of pharmacological therapeutics in human medulloblastoma.
authors.affiliation: Department of Neurosurgery, Fuzhou General Hospital of Nanjing Military Region, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Jing
authors.affiliation: Department of Pediatric Neurosurgery, The Affiliated Xinhua Hospital of the Medical College, Shanghai Jiaotong University, Shanghai 200092, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pediatrics, Fuzhou General Hospital of Nanjing Military Region, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Fuzhou General Hospital of Nanjing Military Region, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Qingshuang
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Pediatric Neurosurgery, The Affiliated Xinhua Hospital of the Medical College, Shanghai Jiaotong University, Shanghai 200092, P.R. China.
authors.forename: Qinchuan
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Department of Neurosurgery, Fuzhou General Hospital of Nanjing Military Region, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Shousen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Pediatric Neurosurgery, The Affiliated Xinhua Hospital of the Medical College, Shanghai Jiaotong University, Shanghai 200092, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015870:Gene Expression; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D006801:Humans; D008527:Medulloblastoma / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014410:Tumor Stem Cell Assay; D055517:snRNP Core Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D055517:snRNP Core Proteins