title: NeuO: a fluorescent chemical probe for live neuron labeling.
abstract: To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 2, 117543 Singapore (Singapore).
authors.forename: Jun Cheng
authors.initials: JC
authors.lastname: Er
authors.forename: Cheryl
authors.initials: C
authors.lastname: Leong
authors.forename: Chai Lean
authors.initials: CL
authors.lastname: Teoh
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yuan
authors.forename: Paolomi
authors.initials: P
authors.lastname: Merchant
authors.forename: Matthew
authors.initials: M
authors.lastname: Dunn
authors.forename: David
authors.initials: D
authors.lastname: Sulzer
authors.forename: Dalibor
authors.initials: D
authors.lastname: Sames
authors.forename: Akshay
authors.initials: A
authors.lastname: Bhinge
authors.forename: Dongyoon
authors.initials: D
authors.lastname: Kim
authors.forename: Seong-Min
authors.initials: SM
authors.lastname: Kim
authors.forename: Myung-Han
authors.initials: MH
authors.lastname: Yoon
authors.forename: Lawrence W
authors.initials: LW
authors.lastname: Stanton
authors.forename: Shawn H
authors.initials: SH
authors.lastname: Je
authors.forename: Seong-Wook
authors.initials: SW
authors.lastname: Yun
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D018613:Microscopy, Confocal; D018715:Microscopy, Video; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes
keywords: BODIPY; fluorescent probes; imaging agents; live neurons