title: Effects of engineered iron nanoparticles on the bryophyte, Physcomitrella patens (Hedw.) Bruch & Schimp, after foliar exposure.
abstract: The effects of iron nanoparticles on bryophytes (Physcomitrella patens) were studied following foliar exposure. We used iron nanoparticles (Fe-NP) representative of industrial emissions from the metallurgical industries. After a characterization of iron nanoparticles and the validation of nanoparticle internalization in cells, the effects (cytotoxicity, oxidative stress, lipid peroxidation of membrane) of iron nanoparticles were determined through the axenic culturing of Physcomitrella patens exposed at five different concentrations (5 ng, 50 ng, 500 ng, 5 µg and 50 µg per plant). Following exposure, the plant health, measured as ATP concentrations, was not impacted. Moreover, we studied oxidative stress in three ways: through the measure of reactive oxygen species (ROS) production, through malondialdehyde (MDA) production and also through glutathione regulation. At concentrations tested over a short period, the level of ROS, MDA and glutathione were not significantly disturbed.
authors.affiliation: Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire des Sciences Végétales et Fongiques, EA4483, 3 rue du Professeur Laguesse - BP83, 59006 Lille cedex, France. Electronic address: ludivine.canivet@univ-lille2.fr.
authors.forename: L
authors.initials: L
authors.lastname: Canivet
authors.affiliation: MCMC - ICMPE UMR 7182, rue H. Dunant, 94320 Thiais, France.
authors.forename: P
authors.initials: P
authors.lastname: Dubot
authors.affiliation: Faculté des Sciences Pharmaceutiques et Biologiques, Département de Toxicologie, Santé publique et Environnement, EA4483, 3 rue du Professeur Laguesse - BP83, 59006 Lille cedex, France.
authors.forename: G
authors.initials: G
authors.lastname: Garçon
authors.affiliation: Faculté des Sciences Pharmaceutiques et Biologiques, Laboratoire des Sciences Végétales et Fongiques, EA4483, 3 rue du Professeur Laguesse - BP83, 59006 Lille cedex, France.
authors.forename: F-O
authors.initials: FO
authors.lastname: Denayer
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D044002:Bryophyta / Q000378:metabolism; D019068:Bryopsida / Q000187:drug effects* / Q000378:metabolism; D055993:Germ Cells, Plant / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007501:Iron / Q000737:chemistry / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects; D010944:Plants / Q000378:metabolism; D026361:Reactive Nitrogen Species / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D007501:Iron; D005978:Glutathione
keywords: Glutathione; Iron nanoparticles; Malondialdehyde; Physcomitrella patens; Reactive oxygen species