title: The EPA2 adhesin encoding gene is responsive to oxidative stress in the opportunistic fungal pathogen Candida glabrata.
abstract: Candida glabrata has emerged as an important opportunistic pathogen in both mucosal and bloodstream infections. C. glabrata contains 67 adhesin-like glycosylphosphatidylinositol-cell-wall proteins (GPI-CWPs), which are classified into seven groups and the largest is the Epa family. Epa proteins are very diverse and their expression is differentially regulated. Like many of the EPA genes, EPA2 is localized in a subtelomeric region where it is subject to chromatin-based transcriptional silencing and its role remains largely unexplored. In this study, we show that EPA2 gene is induced specifically in vitro in the presence of oxidative stress generated by H2O2. This induction is dependent on both Yap1 and Skn7, whereas Msn4 represses EPA2 expression. Interestingly, EPA2 is not induced during phagocytosis, but its expression can be identified in the liver in a murine model of systemic infection. Epa2 has no effect on the virulence of C. glabrata. The work presented herein provides a foundation for future studies to dissect the molecular mechanism(s) by which EPA2 of C. glabrata can be induced in the presence of oxidative stress in a region subject to subtelomeric silencing.
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Juárez-Cepeda
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Orta-Zavalza
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Israel
authors.initials: I
authors.lastname: Cañas-Villamar
authors.affiliation: Laboratorio de Biofísica, Instituto de Física, Universidad Autónoma de San Luis Potosí, Av. Manuel Nava # 6, Zona Universitaria, 78290, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Arreola-Gómez
authors.affiliation: Departamento de Fisiología, Escuela de Medicina, Universidad Autónoma de San Luis Potosí, Avenida Venustiano Carranza #2405, Zona Universitaria, 78210, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Gloria Patricia
authors.initials: GP
authors.lastname: Pérez-Cornejo
authors.affiliation: Laboratorio de Biofísica, Instituto de Física, Universidad Autónoma de San Luis Potosí, Av. Manuel Nava # 6, Zona Universitaria, 78290, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Carmen Yudith
authors.initials: CY
authors.lastname: Hernández-Carballo
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Gutiérrez-Escobedo
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico.
authors.forename: Irene
authors.initials: I
authors.lastname: Castaño
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, IPICYT, Camino a la Presa San José, #2055, Col. Lomas 4ª sección., 78216, San Luis Potosí, San Luis Potosí, Mexico. cano@ipicyt.edu.mx.
authors.forename: Alejandro
authors.initials: A
authors.lastname: De Las Peñas
mesh_terms: D000818:Animals; D041221:Candida glabrata / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D002177:Candidiasis / Q000188:drug therapy / Q000382:microbiology; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D051379:Mice; D018384:Oxidative Stress; D010587:Phagocytosis / Q000235:genetics; D016615:Telomere / Q000737:chemistry / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014774:Virulence
chemical_list: D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D014157:Transcription Factors; D006861:Hydrogen Peroxide
keywords: EPA2; H2O2; IVET; Oxidative stress response; Silencing