title: [Regulation of Thl/Th2 cells by T cell-mediated transcription factor in rats with chronic obstructive pulmonary disease].
abstract: OBJECTIVE
  To determine T box expressed in T cells (T bet), GATA binding protein-3 (GATA 3), and retinoid-related orphan nuclear receptor gammat (RORgammat) in rats with chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  Thirty rats were randomly divided into a control and a COPD group. The COPD model was established through smoking and lipopolysaccharide (LPS) tracheal instillation. Pulmonary function of the rats was measured 28 d after the establishment of the COPD model by a spirometer. Enzyme linked immunosorbent assay was performed to detect serum γ interferon (IFN-γ), interleukin (IL)-4, and IL-17. The expressions of T-bet, GATA-3, and RORgammat protein in lung tissues were determined by Western blot.
  
  
  RESULTS
  Compared with the controls, the COPD rats had decreased pulmonary function and expression of serum IL-4, and increased INF-γ, IL- 17, Th1/Th2, T-bet, T bet /GATA-3, and RORgammat protein (P<0. 05). Forced expiratory volume in 0. 3 seconds (FEV 0.3) was negatively correlated with INF γ and T-bet/GATA-3. Forced vital capacity (FVC) was positively correlated with IL 4. FEV0.3/FVC was negatively correlated with Thl/Th2, T-bet and T-bet/GATA-3. Peak expiratory flow (PEF) was negatively correlated with IL-17, T bet, and RORgammat (P<0. 05). Thl/Th2 was positively correlated with T bet/GATA-3. IL-17 was positively correlated with RORgammat. T bet/GATA-3 was positively correlated with RORgammat (P<0. 05).
  
  
  CONCLUSIONS
  Imbalanced regulation of T bet / GATA 3 and RORgammat on Th1/Th2 and Th17 cells is associated with the occurrence of COPD.
authors.affiliation: Department of Traditional Chinese Medicine, First Affiliated  Hospital of Anhui Medical University, Hefei, China.
authors.forename: Cheng-yangi
authors.initials: CY
authors.lastname: Wang
authors.forename: Xiang-guo
authors.initials: XG
authors.lastname: Liu
authors.forename: Chuan-bo
authors.initials: CB
authors.lastname: Wang
authors.forename: Qiao Rue
authors.initials: QR
authors.lastname: Ji
authors.forename: Li
authors.initials: L
authors.lastname: Fang
authors.forename: Ze
authors.initials: Z
authors.lastname: Li Ze geng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D050990:GATA3 Transcription Factor / Q000378:metabolism*; D007371:Interferon-gamma / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008070:Lipopolysaccharides; D008168:Lung / Q000503:physiopathology; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism; D029424:Pulmonary Disease, Chronic Obstructive / Q000276:immunology*; D012333:RNA, Messenger; D051381:Rats; D020825:T-Box Domain Proteins / Q000378:metabolism*; D058408:Th1-Th2 Balance; D058504:Th17 Cells / Q000276:immunology; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; D020381:Interleukin-17; D008070:Lipopolysaccharides; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D012333:RNA, Messenger; C539902:Rorc protein, rat; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007371:Interferon-gamma