title: Protein kinase Ymr291w/Tda1 is essential for glucose signaling in saccharomyces cerevisiae on the level of hexokinase isoenzyme ScHxk2 phosphorylation*.
abstract: The enzyme ScHxk2 of Saccharomyces cerevisiae is a dual-function hexokinase that besides its catalytic role in glycolysis is involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by the phosphorylation of the nuclear fraction of ScHxk2 at serine 15 and the translocation of the phosphoenzyme into the cytosol. Different studies suggest different serine/threonine protein kinases, Ymr291w/Tda1 or Snf1, to accomplish ScHxk2-S15 phosphorylation. The current paper provides evidence that Ymr291w/Tda1 is essential for that modification, whereas protein kinases Ydr477w/Snf1, Ynl307c/Mck1, Yfr014c/Cmk1, and Ykl126w/Ypk1, which are co-purified during Ymr291w/Tda1 tandem affinity purification, as well as protein kinase PKA and PKB homolog Sch9 are dispensable. Taking into account the detection of a significantly higher amount of the Ymr291w/Tda1 protein in cells grown in low-glucose media as compared with a high-glucose environment, Ymr291w/Tda1 is likely to contribute to glucose signaling in S. cerevisiae on the level of ScHxk2-S15 phosphorylation in a situation of limited external glucose availability. The evolutionary conservation of amino acid residue serine 15 in yeast hexokinases and its phosphorylation is illustrated by the finding that YMR291W/TDA1 of S. cerevisiae and the homologous KLLA0A09713 gene of Kluyveromyces lactis allow for cross-complementation of the respective protein kinase single-gene deletion strains.
authors.affiliation: From the Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden.
authors.forename: Sonja
authors.initials: S
authors.lastname: Kaps
authors.affiliation: From the Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Karina.Kettner@tu-dresden.de.
authors.forename: Karina
authors.initials: K
authors.lastname: Kettner
authors.affiliation: the Mass Spectrometry Platform, Max Delbrück Center for Molecular Medicine, 13125 Berlin, and.
authors.forename: Rebekka
authors.initials: R
authors.lastname: Migotti
authors.affiliation: the Mass Spectrometry Platform, Max Delbrück Center for Molecular Medicine, 13125 Berlin, and.
authors.forename: Tamara
authors.initials: T
authors.lastname: Kanashova
authors.affiliation: the Institute of Genetics, Technische Universität Dresden, 01062 Dresden, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Krause
authors.affiliation: the Institute of Genetics, Technische Universität Dresden, 01062 Dresden, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rödel
authors.affiliation: the Mass Spectrometry Platform, Max Delbrück Center for Molecular Medicine, 13125 Berlin, and.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Dittmar
authors.affiliation: From the Institute of Physiological Chemistry, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden.
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Kriegel
mesh_terms: D000595:Amino Acid Sequence; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D006593:Hexokinase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics; D007716:Kluyveromyces; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012694:Serine / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D007527:Isoenzymes; D029701:Saccharomyces cerevisiae Proteins; D012694:Serine; C490214:HXK2 protein, S cerevisiae; D006593:Hexokinase; D017346:Protein Serine-Threonine Kinases; D005947:Glucose
keywords: Hexokinase; Kluyveromyces lactis; Phosphorylation; Protein Kinase A (PKA); Saccharomyces cerevisiae; ScHxk2; Sch9; Signaling; Snf1; Ymr291w/Tda1