title: Detection of mouse endogenous type B astrocytes migrating towards brain lesions.
abstract: Neuroblasts represent the predominant migrating cell type in the adult mouse brain. There are, however, increasing evidences of migration of other neural precursors. This work aims at identifying in vivo endogenous early neural precursors, different from neuroblasts, able to migrate in response to brain injuries. The monoclonal antibody Nilo1, which unequivocally identifies type B astrocytes and embryonic radial glia, was coupled to magnetic glyconanoparticles (mGNPs). Here we show that Nilo1-mGNPs in combination with magnetic resonance imaging in living mice allowed the in vivo identification of endogenous type B astrocytes at their niche, as well as their migration to the lesion site in response to glioblastoma, demyelination, cryolesion or mechanical injuries. In addition, Nilo1(+) adult radial glia-like structures were identified at the lesion site a few hours after damage. For all damage models used, type B astrocyte migration was fast and orderly. Identification of Nilo1(+) cells surrounding an induced glioblastoma was also possible after intraperitoneal injection of the antibody. This opens up the possibility of an early identification of the initial damage site(s) after brain insults, by the migration of type B astrocytes.
authors.affiliation: Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors.forename: Gema
authors.initials: G
authors.lastname: Elvira
authors.affiliation: Laboratory of Glyconanotechnology, CICbiomaGUNE, San Sebastian, Spain; CIBER-BBN, San Sebastian, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: García
authors.affiliation: Laboratory of Glyconanotechnology, CICbiomaGUNE, San Sebastian, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Gallo
authors.affiliation: Medicina y Cirugía Experimental, Hospital General Universitario Gregorio Marañón, CIBERSAM, Madrid, Spain.
authors.forename: Marina
authors.initials: M
authors.lastname: Benito
authors.affiliation: Department of Bioengineering and Aerospace Engineering, Universidad Carlos III, Madrid, Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Montesinos
authors.affiliation: Centro Integral Oncológico Clara Campal (CIOCC) and Instituto de Medicina Molecular Aplicada (IMMA), Hospital de Madrid Foundation, Madrid, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Holgado-Martin
authors.affiliation: Centro Integral Oncológico Clara Campal (CIOCC) and Instituto de Medicina Molecular Aplicada (IMMA), Hospital de Madrid Foundation, Madrid, Spain.
authors.forename: Angel
authors.initials: A
authors.lastname: Ayuso-Sacido
authors.affiliation: Laboratory of Glyconanotechnology, CICbiomaGUNE, San Sebastian, Spain; CIBER-BBN, San Sebastian, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Penadés
authors.affiliation: Medicina y Cirugía Experimental, Hospital General Universitario Gregorio Marañón, CIBERSAM, Madrid, Spain; Department of Bioengineering and Aerospace Engineering, Universidad Carlos III, Madrid, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Desco
authors.affiliation: Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain.
authors.forename: Augusto
authors.initials: A
authors.lastname: Silva
authors.affiliation: Centro de Investigaciones Biológicas (CIB-CSIC), Madrid, Spain. Electronic address: jasanz@cib.csic.es.
authors.forename: Jose A
authors.initials: JA
authors.lastname: Garcia-Sanz
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001253:Astrocytes / Q000166:cytology* / Q000637:transplantation; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001930:Brain Injuries / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000166:cytology / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D014411:Neoplastic Stem Cells / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D058185:Magnetite Nanoparticles