title: Carbohydrates as new probes for the identification of closely related Escherichia coli strains using surface plasmon resonance imaging.
abstract: Prevention of foodborne diseases depends highly on our ability to control rapidly and accurately a possible contamination of food. So far, standard procedures for bacterial detection require time-consuming bacterial cultures on plates before the pathogens can be detected and identified. We present here an innovative biochip, based on direct differential carbohydrate recognitions of five closely related Escherichia coli strains, including the enterohemorragic E. coli O157:H7. Our device relies on efficient grafting of simple carbohydrates on a gold surface and on the monitoring of their interactions with bacteria during their culture using surface plasmon resonance imaging. We show that each of the bacteria interacts in a different way with the carbohydrate chip. This allows the detection and discrimination of the tested bacterial strains in less than 10 h from an initial bacterial concentration of 10(2) CFU·mL(-1). This is an improvement over previously described systems in terms of cost, easiness to use, and stability. Easily conceived and easily regenerated, this tool is promising for the future of food safety.
authors.affiliation: Univ. Grenoble Alpes, INAC-SPRAM , F-38000 Grenoble, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Bulard
authors.forename: Aurélie
authors.initials: A
authors.lastname: Bouchet-Spinelli
authors.forename: Patricia
authors.initials: P
authors.lastname: Chaud
authors.forename: André
authors.initials: A
authors.lastname: Roget
authors.forename: Roberto
authors.initials: R
authors.lastname: Calemczuk
authors.forename: Sébastien
authors.initials: S
authors.lastname: Fort
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
mesh_terms: D050260:Carbohydrate Metabolism; D015169:Colony Count, Microbial; D004867:Equipment Design; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000175:diagnosis / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D059022:Food Safety; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D006801:Humans; D046228:Microarray Analysis / Q000295:instrumentation*; D015335:Molecular Probes / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D046888:Tissue Array Analysis / Q000295:instrumentation
chemical_list: D015335:Molecular Probes