title: Origins of the breast milk-derived cells; an endeavor to find the cell sources.
abstract: Fresh human breast milk consists of a heterogeneous population of cells that may offer a non-invasive source of cells for therapeutic proposes. The aims of this study were to characterize the breast milk-derived cells cultured in vitro. To do this, the cells from human breast milk were cultured and the expression of the CD markers along with the embryonic stem cell markers, endothelial and luminal mammary epithelial cell markers was evaluated by flow cytometry and immunofluorescence. The presence of fetal microchimerism among the isolated cells was also determined by the presence of SRY gene. They were also differentiated into adipocytes and osteoblasts. The results showed that a remarkable number of cells expressed the mesenchymal stem cell (MSC) markers such as CD90, CD44, CD271, and CD146. A subpopulation of the human breast milk-derived cells (HBMDC) also expressed the embryonic stem cell markers, such as TRA 60-1, Oct4, Nanog and Sox2 but not SSEA1 or 4. The frequencies of the cells which expressed the endothelial, hematopoietic cell markers were negligible. SRY gene was not detected in the breast milk isolated cells. A subpopulation of the cells also expressed cytokeratin 18, the marker of luminal mammary epithelial cells. These cells showed the capability to differentiate into adipocytes and osteoblasts. In conclusion, these finding highlighted the presence of cells with various sources in the breast milk. Different stem cells including MSCs or embryonic stem cell-like cell along with the exfoliated cells from luminal epithelial cells were found among the isolated cells. The breast milk-derived stem cells might be considered as a non-invasive source of the stem cells for therapeutic purpose.
authors.affiliation: Anatomy Department, Laboratory for stem cell research, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran; Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran; Cellular and Molecular Research Club, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Sani
authors.forename: Seyed Mojtaba
authors.initials: SM
authors.lastname: Hosseini
authors.forename: Mahin
authors.initials: M
authors.lastname: Salmannejad
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Aleahmad
authors.forename: Sepideh
authors.initials: S
authors.lastname: Ebrahimi
authors.forename: Samira
authors.initials: S
authors.lastname: Jahanshahi
authors.forename: Tahereh
authors.initials: T
authors.lastname: Talaei-Khozani
mesh_terms: D017667:Adipocytes / Q000502:physiology; D000328:Adult; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D042361:Mammary Glands, Human / Q000166:cytology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D008895:Milk, Human / Q000166:cytology*; D010006:Osteoblasts / Q000502:physiology; D055815:Young Adult
keywords: cell differentiation; human breast milk; mammary gland; mammary stem cell microchimerism