title: Determination of metal content in superoxide dismutase enzymes by capillary electrophoresis†.
abstract: Superoxide dismutases are antioxidant scavenger enzymes that contain a metal cofactor (copper, zinc, iron, and manganese) in their active site. Metal content measurement is one of the essential steps to characterize enzyme biological activity. We have developed a capillary electrophoretic protocol for the determination of the metal content in superoxide dismutase enzymes. The background electrolyte containing 10 mM pyridine-2,6-dicarboxylic acid and 1 mM 1-methyl-3-tetradecylimidazolium chloride at pH 3.8 was optimized for on-column complexation of the above-mentioned metals. The minimum detectable levels of metals ranged from 0.3 to 1.2 μg/mL. The reliability of the method was checked by parallel quantitative determination of the metal content in superoxide dismutase enzymes by graphite furnace or flame atomic absorption spectrophotometry methods.
authors.affiliation: Department of Chemistry, Tallinn University of Technology, Tallinn, Estonia.
authors.forename: Jana
authors.initials: J
authors.lastname: Kazarjan
authors.forename: Merike
authors.initials: M
authors.lastname: Vaher
authors.forename: Thérèse
authors.initials: T
authors.lastname: Hunter
authors.forename: Maria
authors.initials: M
authors.lastname: Kulp
authors.forename: Gary James
authors.initials: GJ
authors.lastname: Hunter
authors.forename: Rosalin
authors.initials: R
authors.lastname: Bonetta
authors.forename: Diane
authors.initials: D
authors.lastname: Farrugia
authors.forename: Mihkel
authors.initials: M
authors.lastname: Kaljurand
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D002417:Cattle; D003300:Copper / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D007501:Iron / Q000032:analysis*; D008345:Manganese / Q000032:analysis*; D013482:Superoxide Dismutase / Q000737:chemistry*; D015032:Zinc / Q000032:analysis*
chemical_list: D029742:Caenorhabditis elegans Proteins; D008345:Manganese; D003300:Copper; D007501:Iron; D013482:Superoxide Dismutase; D015032:Zinc
keywords: Capillary electrophoresis; On-column complexation; Quantitative metal analysis; Superoxide dismutase