title: Comparison of cryopreservation bags for hematopoietic progenitor cells using a WBC-enriched product.
abstract: Hematopoietic progenitor cells (HPC) are stored in cryopreservation bags that are resistant to liquid nitrogen. Since Cryocyte bags of Baxter (B-bags) are no longer available, an alternative bag was sought. Also, the influence of freezing volume was studied. Miltenyi Biotec (MB)- and MacoPharma (MP)-bags passed the integrity tests without failure. Comparing MB- and MP-bags with B-bags, no difference in WBC recovery or viability was found when using a WBC-enriched product as a "dummy" HPC product. Further, a freezing volume of 30 mL resulted in better WBC recovery and viability than 60 mL. Additonal studies using real HPC might be necessary.
authors.affiliation: Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands. Electronic address: margriet.j.dijkstra@gmail.com.
authors.forename: Margriet J
authors.initials: MJ
authors.lastname: Dijkstra-Tiekstra
authors.affiliation: Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands.
authors.forename: Sandra
authors.initials: S
authors.lastname: Hazelaar
authors.affiliation: Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands.
authors.forename: Effimia
authors.initials: E
authors.lastname: Gkoumassi
authors.affiliation: Sanquin Blood Supply, Division Blood Bank, Stem Cell Laboratory, Groningen, The Netherlands.
authors.forename: Margienus
authors.initials: M
authors.lastname: Weggemans
authors.affiliation: Sanquin Blood Supply, Department Transfusion Monitoring, Groningen, The Netherlands.
authors.forename: Janny
authors.initials: J
authors.lastname: de Wildt-Eggen
mesh_terms: D001793:Blood Preservation / Q000379:methods; D002470:Cell Survival; D015925:Cryopreservation / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005615:Freezing; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology*; D013048:Specimen Handling
keywords: Cryopreservation bags; Integrity; WBC recovery; WBC viability; WBC-enriched product