title: Effects of Lactobacillus acidophilus dietary supplementation on the performance, intestinal barrier function, rectal microflora and serum immune function in weaned piglets challenged with Escherichia coli lipopolysaccharide.
abstract: This study was conducted with a lipopolysaccharide (LPS)-challenged piglet model to determine the effects of diets containing Lactobacillus acidophilus on the performance, intestinal barrier function, rectal microflora and serum immune function. A total of 150 piglets (initial body weight (BW) 7.53 ± 0.21 kg) were allotted to one of the following diets, including a basal diet, a basal diet supplemented with 250 mg/kg Flavomycin, or basal diet plus 0.05, 0.1 or 0.2 % L. acidophilus. On day 28 of the trial, the pigs were given an intraperitoneal injection of LPS (200 μg/kg body weight) followed by blood collection 3 h later. Diets with either antibiotics, 0.1 or 0.2 % Lactobacillus increased (P < 0.05) the final BW and decreased (P < 0.05) feed gain ratio (F/G) compared with the control group. Pigs fed diets containing antibiotic or Lactobacillus had greater average daily gain (ADG) (P < 0.05) than pigs fed the control diet. The rectal content Lactobacillus counts for pigs fed diet containing Lactobacillus were significant higher (P < 0.01) than those fed antibiotic or control diet. Feeding the Lactobacillus diets decreased the Escherichia coli counts of rectal content (P < 0.01). Pigs fed diets containing 0.1 or 0.2 % Lactobacillus decreased serum DAO activity (P < 0.05) compared with pigs fed the control diet. Serum IL-10 concentration was enhanced in pigs fed the diet with Lactobacillus compared to pigs fed the control diet and antibiotic diet. Feeding a diet with Lactobacillus reduced (P < 0.05) IFN-γ concentration compared to the control diet. Inclusion of Lactobacillus in diets fed to pigs reduced TNF-α concentration compared with pigs fed no Lactobacillus (P < 0.05). These results indicate that feeding with L. acidophilus improved growth performance and protected against LPS-induced inflammatory status.
authors.affiliation: Tianjin Institute of Animal Husbandry and Veterinary Medicine, 17 km point of Jin-Jing Road, Tianjin, 300381, People's Republic of China, qiaojy1979@126.com.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Qiao
authors.forename: Haihua
authors.initials: H
authors.lastname: Li
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D016207:Cytokines / Q000097:blood; D019587:Dietary Supplements; D004926:Escherichia coli / Q000276:immunology*; D000069196:Gastrointestinal Microbiome; D007422:Intestines / Q000276:immunology*; D007779:Lactobacillus acidophilus / Q000276:immunology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D044967:Serum / Q000276:immunology*; D013552:Swine
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides