title: Measuring helicase inhibition of the DEAD-box protein Dbp2 by Yra1.
abstract: Despite the highly conserved helicase core, individual DEAD-box proteins are specialized in diverse RNA metabolic processes. One mechanism that determines DEAD-box protein specificity is enzymatic regulation by other protein cofactors. In this chapter, we describe a protocol for purifying the Saccharomyces cerevisiae DEAD-box RNA helicase Dbp2 and RNA-binding protein Yra1 and subsequent analysis of helicase regulation. The experiments described here can be adapted to other RNA helicases and their purified cofactor(s).
authors.affiliation: Department of Biochemistry, Purdue University, BCHM 305, 175 S. University Street, West Lafayette, IN, 47907-2063, USA.
authors.forename: Wai Kit
authors.initials: WK
authors.lastname: Ma
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Tran
mesh_terms: D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; C105887:YRA1 protein, S cerevisiae; C438898:Dbp2 protein, S cerevisiae; D053487:DEAD-box RNA Helicases