title: A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants.
abstract: Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.
authors.affiliation: 1] Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France [2] Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
authors.forename: Antoine
authors.initials: A
authors.lastname: Larrieu
authors.affiliation: 1] Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France [2] Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux, CP 18534 Dakar, Senegal.
authors.forename: Antony
authors.initials: A
authors.lastname: Champion
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Legrand
authors.affiliation: Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Lavenus
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France.
authors.forename: David
authors.initials: D
authors.lastname: Mast
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Brunoud
authors.affiliation: Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
authors.forename: Jaesung
authors.initials: J
authors.lastname: Oh
authors.affiliation: Université Montpellier 2, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 34394 Montpellier, France.
authors.forename: Soazig
authors.initials: S
authors.lastname: Guyomarc'h
authors.affiliation: Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Pizot
authors.affiliation: Department of Plant Molecular Biology, Université de Lausanne, 1015 Lausanne, Switzerland.
authors.forename: Edward E
authors.initials: EE
authors.lastname: Farmer
authors.affiliation: Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Colin
authors.initials: C
authors.lastname: Turnbull
authors.affiliation: Laboratoire de Reproduction et Développement des Plantes, CNRS, INRA, ENS Lyon, UCBL, Université de Lyon, 69364 Lyon, France.
authors.forename: Teva
authors.initials: T
authors.lastname: Vernoux
authors.affiliation: Centre for Plant Integrative Biology, University of Nottingham, Nottingham LE12 5RD, UK.
authors.forename: Malcolm J
authors.initials: MJ
authors.lastname: Bennett
authors.affiliation: 1] Institut de Recherche pour le Développement, Unité Mixte de Recherche Diversité Adaptation et Développement des plantes, 911 Avenue Agropolis, 34394 Montpellier, France [2] Laboratoire mixte international Adaptation des Plantes et microorganismes associés aux Stress Environnementaux, CP 18534 Dakar, Senegal.
authors.forename: Laurent
authors.initials: L
authors.lastname: Laplaze
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D018548:Cotyledon / Q000378:metabolism; D003517:Cyclopentanes / Q000032:analysis* / Q000378:metabolism*; D054883:Oxylipins / Q000032:analysis* / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D010944:Plants / Q000378:metabolism*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid