title: Tri-peptide cationic lipids for gene delivery.
abstract: Several novel tri-peptide cationic lipids were designed and synthesized for delivering DNA and siRNA. They have tri-lysine and tri-ornithine as head groups, carbamate group as linker and 12 and 14 carbon atom alkyl groups as tails. These tri-peptide cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. Their particle size, Zeta potential and DNA-binding were characterized to show that they were suitable for gene transfection. The further results indicate that these lipids can transfer DNA and siRNA very efficiently into NCI-H460 and Hep-2 tumor cells. The selected lipid, CDO14, was able to deliver combined siRNAs against c-Myc and VEGF for silencing distinct oncogenic pathways in lung tumors of mice, with littlein vitroandin vivotoxicity.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116021, Liaoning, China ; SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, Dalian Nationalities University, Dalian 116600, Liaoning, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, Dalian Nationalities University, Dalian 116600, Liaoning, China.
authors.forename: Shubiao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Materials Science and Engineering, Department of Biological Engineering, The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, Dalian Nationalities University, Dalian 116600, Liaoning, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Cui
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, Dalian Nationalities University, Dalian 116600, Liaoning, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Chen
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bio-resources Utilization, Dalian Nationalities University, Dalian 116600, Liaoning, China.
authors.forename: Defu
authors.initials: D
authors.lastname: Zhi
authors.affiliation: College of Phamacy, Dalian Medical University, Dalian 116044, Liaoning, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116021, Liaoning, China.
authors.forename: Shufen
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Division of Molecular Pharmaceutics, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
authors.forename: Leaf
authors.initials: L
authors.lastname: Huang