title: Cystain C and neuropeptid Y levels in brain tissues after experimental subarachnoid hemorrhage.
abstract: The aim of this study was to investigate the changes in the levels of cystatin C, which protects neurodegeneration in the central nervous system with the inhibition of cysteine protease and by inducing autophagy in the pathogenesis of cerebral vasospasm and levels of vasoconstrictive neuropeptid Y (NPY) in the brain tissue homogenates of rat model of subarachnoid hemorrhage (SAH). Three experimental groups were used: Day 2 and Day 7 groups after SAH, and also a control group. There were seven Wistar albino rats in each group. SAH was accomplished by transclival basilar artery puncture. Rat cystatin C, rat NPY were determined with ELISA in brain tissue homogenates. Day 2 group showed significantly enhanced cystatin C values in comparision with the control group (P=0.048). NPY levels between the Day 2 and Day 7 groups and the control groups were not significantly different (P=0.315). In histopathological examination, there was less neuronal loss in the Day 2 group than in the Day 7 group. Regarding our results, it would be more valuable to measure NPY levels in specific brain areas. The increased cystatin C levels on the second day after SAH is probably a pathophysiologic mechanism to organize protease activity.
authors.affiliation: Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Şerefden
authors.initials: Ş
authors.lastname: Açıkgöz
authors.affiliation: Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Can
authors.affiliation: Department of Biochemistry, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Berrak
authors.initials: B
authors.lastname: Güven
authors.affiliation: Department Neurosurgery, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Nurullah
authors.initials: N
authors.lastname: Edebali
authors.affiliation: Department of Pathology, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Figen
authors.initials: F
authors.lastname: Barut
authors.affiliation: Department of Biostatistics, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Çağatay
authors.initials: Ç
authors.lastname: Büyükuysal
authors.affiliation: Department of Immunology Faculty of Medicine, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: İshak Özel
authors.initials: İÖ
authors.lastname: Tekin
authors.affiliation: Department Neurosurgery, Bülent Ecevit University (Formerly, Zonguldak Karaelmas University), Zonguldak, Turkey.
authors.forename: Bektaş
authors.initials: B
authors.lastname: Açıkgöz
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D055316:Cystatin C / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism*; D051381:Rats; D013345:Subarachnoid Hemorrhage / Q000378:metabolism*
chemical_list: D055316:Cystatin C; D009478:Neuropeptide Y