title: A clinical correlation of anti-DNA-AGE autoantibodies in type 2 diabetes mellitus with disease duration.
abstract: Nonenzymatic glycation of amino groups of DNA bases by reducing sugars can generate advanced glycation end products (AGEs). Cellular formation of AGEs under normal physiology is continuously scanned and removed by efficient system in the cells. However, excess formation and accumulation of AGEs may be cause or consequence of some human diseases. Mammalian DNA incubated with d-glucose for 28 days at 37°C showed structural changes in DNA as confirmed by UV, fluorescence, CD, melting temperature, S1 nuclease sensitivity and gel electrophoresis. Formation of DNA-AGE was confirmed by HPLC and LC-MS. Enzyme immunoassay and electrophoretic mobility shift assay of autoantibodies in type 2 diabetes patients' sera with disease duration of 5-15 years exhibited significantly high binding with DNA-AGE as compared to patients with 1-5 years of disease duration. Autoantibodies against aberrant DNA-AGE may be important in the assessment of initiation/progression of secondary complications in type 2 diabetes mellitus patients.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India.
authors.forename: Jalaluddin M
authors.initials: JM
authors.lastname: Ashraf
authors.affiliation: Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India.
authors.forename: Mir Yasir
authors.initials: MY
authors.lastname: Arfat
authors.affiliation: R.G. Centre for Diabetes and Endocrinology, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India.
authors.forename: Zarina
authors.initials: Z
authors.lastname: Arif
authors.affiliation: R.G. Centre for Diabetes and Endocrinology, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India.
authors.forename: Jamal
authors.initials: J
authors.lastname: Ahmad
authors.affiliation: Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India.
authors.lastname: Moinuddin
authors.affiliation: Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh-202002, UP, India. Electronic address: kalam786@rediffmail.com.
authors.forename: Khursheed
authors.initials: K
authors.lastname: Alam
mesh_terms: D000328:Adult; D000368:Aged; D050197:Atherosclerosis / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D015415:Biomarkers / Q000097:blood; D004247:DNA / Q000276:immunology / Q000648:ultrastructure; D003924:Diabetes Mellitus, Type 2 / Q000276:immunology*; D003928:Diabetic Nephropathies / Q000276:immunology*; D003930:Diabetic Retinopathy / Q000276:immunology*; D024202:Electrophoretic Mobility Shift Assay; D005260:Female; D017127:Glycation End Products, Advanced / Q000276:immunology*; D006801:Humans; D008297:Male; D009690:Nucleic Acid Conformation; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001323:Autoantibodies; D015415:Biomarkers; D017127:Glycation End Products, Advanced; D004247:DNA
keywords: Autoantibodies; CEdG; DNA; Glucose; Glycation; Type 2 diabetes