title: Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.
abstract: Human dipeptidyl peptidase IV (hDPPIV) inhibitors provide an effective strategy for the treatment of type 2 diabetes. Because certain peptides are known to act as hDPPIV inhibitors, a dataset of possible peptides with their inhibition intensities will facilitate the development of functional food for type 2 diabetes. In this study, we examined a total of 337 dipeptides with respect to their hDPPIV inhibitory effects. Amino acid residues at N-termini dominated their inhibition intensities. Particularly highly inhibitory dipeptides discovered included the following novel dipeptides: Thr-His, Asn-His, Val-Leu, Met-Leu, and Met-Met. Using our dataset, prime candidates contributing to the hDPPIV inhibitory effect of soy protein hydrolyzates were successfully identified. Possible dietary proteins potentially able to produce particularly highly hDPPIV inhibitory peptides are also discussed on the basis of the dataset.
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: s13211@u-shizuoka-ken.ac.jp.
authors.forename: Vu Thi Tuyet
authors.initials: VT
authors.lastname: Lan
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan; School of Food and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: sukeito@u-shizuoka-ken.ac.jp.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ito
authors.affiliation: School of Food and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: f11005@u-shizuoka-ken.ac.jp.
authors.forename: Masumi
authors.initials: M
authors.lastname: Ohno
authors.affiliation: Food Science Research Institute, Research & Development HQ, Fuji Oil Co., Ltd., Kinunodai 4-3, Tsukubamirai, Ibaraki 300-2497, Japan. Electronic address: motoyama.takayasu@so.fujioil.co.jp.
authors.forename: Takayasu
authors.initials: T
authors.lastname: Motoyama
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan; School of Food and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: itosohei@u-shizuoka-ken.ac.jp.
authors.forename: Sohei
authors.initials: S
authors.lastname: Ito
authors.affiliation: Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan; School of Food and Nutritional Sciences, University of Shizuoka, Yada 52-1, Suruga-ku, Shizuoka 422-8526, Japan. Electronic address: kawarsky@u-shizuoka-ken.ac.jp.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawarasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004044:Dietary Proteins / Q000032:analysis / Q000737:chemistry; D004151:Dipeptides / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D030262:Soybean Proteins / Q000032:analysis / Q000737:chemistry
chemical_list: D004044:Dietary Proteins; D004151:Dipeptides; D054873:Dipeptidyl-Peptidase IV Inhibitors; D019151:Peptide Library; D030262:Soybean Proteins
keywords: Dipeptide; Functional food; Human dipeptidyl peptidase IV; Type 2 diabetes