title: Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug.
abstract: We synthesized a cytoprotective magnetic nanoparticle by reacting a maleimide functionalized Feraheme (FH) with a disulfide linked dimer of a polyhis tagged annexin V. Following reductive cleavage of disulfide, the resulting annexin-nanoparticle (diameter = 28.0 ± 2.0 nm by laser light scattering, 7.6 annexin's/nanoparticle) was cytoprotective to cells subjected to plasma membrane disrupting chemotherapeutic or mechanical stresses, and significantly more protective than the starting annexin V. Annexin-nanoparticles provide an approach to the design of nanomaterials which antagonize the plasma membrane permeability characteristic of necrosis and which may have applications as cytoprotective agents.
authors.affiliation: Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Boston, MA 02129, USA.
authors.forename: Howard H
authors.initials: HH
authors.lastname: Chen
authors.forename: Hushan
authors.initials: H
authors.lastname: Yuan
authors.forename: Hoonsung
authors.initials: H
authors.lastname: Cho
authors.forename: David E
authors.initials: DE
authors.lastname: Sosnovik
authors.forename: Lee
authors.initials: L
authors.lastname: Josephson
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000737:chemistry*; D000970:Antineoplastic Agents / Q000737:chemistry; D017209:Apoptosis; D016466:CHO Cells; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D019281:Dimerization; D004220:Disulfides / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D008027:Light; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry*; D009336:Necrosis; D010316:Particle Size; D010539:Permeability; D012542:Scattering, Radiation; D013314:Stress, Mechanical
chemical_list: D017304:Annexin A5; D000970:Antineoplastic Agents; D004220:Disulfides; D004337:Drug Carriers; D052203:Ferrosoferric Oxide