title: miR-25 modulates NSCLC cell radio-sensitivity through directly inhibiting BTG2 expression.
abstract: A large proportion of the NSCLC patients were insensitive to radiotherapy, but the exact mechanism is still unclear. This study explored the role of miR-25 in regulating sensitivity of NSCLC cells to ionizing radiation (IR) and its downstream targets. Based on measurement in tumor samples from NSCLC patients, this study found that miR-25 expression is upregulated in both NSCLC and radio-resistant NSCLC patients compared the healthy and radio-sensitive controls. In addition, BTG expression was found negatively correlated with miR-25a expression in the both tissues and cells. By applying luciferase reporter assay, we verified two putative binding sites between miR-25 and BTG2. Therefore, BTG2 is a directly target of miR-25 in NSCLC cancer. By applying loss-and-gain function analysis in NSCLC cell lines, we demonstrated that miR-25-BTG2 axis could directly regulated BTG2 expression and affect radiotherapy sensitivity of NSCLC cells.
authors.affiliation: Pneumology Department, Civil Aviation General Hospital, Beijing 100123, China. Electronic address: carlhe@126.com.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: He
authors.affiliation: Pneumology Department, Civil Aviation General Hospital, Beijing 100123, China. Electronic address: cassieliu@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Pneumology Department, Civil Aviation General Hospital, Beijing 100123, China. Electronic address: rockg714@aliyun.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Xiao
authors.affiliation: Pneumology Department, Civil Aviation General Hospital, Beijing 100123, China. Electronic address: xiaosenqian@126.com.
authors.forename: Xiaosen
authors.initials: X
authors.lastname: Qian
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001665:Binding Sites / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000532:radiotherapy*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000532:radiotherapy*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011836:Radiation Tolerance / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D017874:Immediate-Early Proteins; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; D025521:Tumor Suppressor Proteins; C070114:BTG2 protein, human
keywords: BTG2; NSCLC; Radio-sensitivity; miR-25