title: Mutations in HSP70-2 gene change the susceptibility to clinical mastitis in Chinese Holstein.
abstract: To select the molecular markers susceptible to mastitis and reduce the loss induced by mastitis, the PCR-SSCP method was adopted to investigate the correlation between SNPs of the HSP70-2 gene and mastitis in 103 Chinese Holstein. 25 new polymorphisms were detected in this study: 9 SNPs (g.-115 G→A, g.-98 T→C, g.117 C→A, g.156 A→C, g.1743 G→A, g.1746 C→T, g.1761 T→G, g.1770 C→G, g.1877 G→C) were found to be associated with mastitis; 2 key SNPs led to amino-acid changes: g.1770 C→G (590 Asp→Glu), g.1877 G→C (626 Gly→Ala). 626 Gly→Ala affected the protein secondary structure. 3 potential cow HSP70-2 proteins were found in all the 103 individuals, but predicted three-dimensional structures of 3 proteins are the same with each other. It is suggested that 9 SNPs increase the susceptibility to mastitis due to their low polymorphisms and can be used as molecular markers to breed the dairy cows resistant to mastitis.
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Pin
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Chunwan
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Zaiqun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Huo
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Honglin
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Georgia, Georgia Regents University, Augusta, GA 30912, USA.
authors.forename: Yong
authors.initials: Y
authors.lastname: Teng
authors.affiliation: College of Life Sciences, Anhui Normal University, Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, Wuhu 241000, Anhui, China. Electronic address: caiyafeinau@163.com.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Cai
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002417:Cattle; D005260:Female; D020022:Genetic Predisposition to Disease; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D008414:Mastitis, Bovine / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017433:Protein Structure, Secondary / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins
keywords: Chinese Holstein; HSP70-2; Mastitis; SNPs