title: Different requirements for Wnt signaling in tongue myogenic subpopulations.
abstract: The tongue is a muscular organ that is essential in vertebrates for important functions, such as food intake and communication. Little is known about regulation of myogenic progenitors during tongue development when compared with the limb or trunk region. In this study, we investigated the relationship between different myogenic subpopulations and the function of canonical Wnt signaling in regulating these subpopulations. We found that Myf5- and MyoD-expressing myogenic subpopulations exist during embryonic tongue myogenesis. In the Myf5-expressing myogenic progenitors, there is a cell-autonomous requirement for canonical Wnt signaling for cell migration and differentiation. In contrast, the MyoD-expressing subpopulation does not require canonical Wnt signaling during tongue myogenesis. Taken together, our results demonstrate that canonical Wnt signaling differentially regulates the Myf5- and MyoD-expressing subpopulations during tongue myogenesis.
authors.affiliation: Department of Orthodontics, School of Stomatology, Peking University, Beijing, China Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mayo
authors.affiliation: Center for Craniofacial Molecular Biology, Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA ychai@usc.edu.
authors.forename: Y
authors.initials: Y
authors.lastname: Chai
mesh_terms: D000818:Animals; D060466:Axin Protein / Q000032:analysis / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D051379:Mice; D024510:Muscle Development / Q000502:physiology*; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D017570:MyoD Protein / Q000032:analysis / Q000502:physiology; D051787:Myogenic Regulatory Factor 5 / Q000032:analysis / Q000502:physiology; D022661:RNA, Untranslated / Q000032:analysis / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*; D014059:Tongue / Q000166:cytology / Q000196:embryology*; D060449:Wnt Signaling Pathway / Q000502:physiology*; D051176:beta Catenin / Q000032:analysis / Q000502:physiology
chemical_list: D060466:Axin Protein; C111729:Axin2 protein, mouse; C495267:CTNNB1 protein, mouse; C110853:Gt(ROSA)26Sor non-coding RNA, mouse; C497418:Myf5 protein, mouse; D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D051787:Myogenic Regulatory Factor 5; D022661:RNA, Untranslated; D051176:beta Catenin
keywords: Myf5; MyoD; Wnt/β-catenin; craniofacial muscles; myogenesis; progenitors