title: Association of matrix metalloproteinase-7 (-181A/G) promoter polymorphism in chronic pancreatitis.
abstract: OBJECTIVE
  Chronic pancreatitis is progressive and irreversible destruction of the pancreas. Matrix metalloproteinase-7 (MMP-7) is a secreted matrilysin, which contributes to angiogenesis and breakdown of basement membranes of pancreatic tissues. The present study was aimed to investigate the association of MMP-7 -181A/G (rs11568818) gene promoter polymorphism in patients with chronic pancreatitis.
  
  
  METHODS
  A total of 100 chronic pancreatitis patients and 150 unrelated healthy individuals were included in this case control study. The genotyping of the MMP-7 gene (- 181 A/G) (rs11568818) was carried out based on PCR-RFLP. The serum levels of MMP-7 were determined by ELISA. Association between genotypes and chronic pancreatitis was examined by odds ratio (OR) with 95% confidence interval (CI).
  
  
  RESULTS
  The frequencies of the genotypes in promoter of MMP-7 were AA 49 per cent, AG 25 per cent and GG 26 per cent in chronic pancreatitis patients and AA 53 per cent, AG 38 per cent and GG 9 per cent in control subjects. Frequency of MMP-7 -181GG genotype and - 181G allele was significantly associated with chronic pancreatitis compared to healthy subjects [OR = 1.58 (95% CI: 1.06 -2.36), p =0.019]. There was no significant difference in the serum MMP-7 levels in the patients compared to control subjects.
  
  
  CONCLUSIONS
  The present study revealed a significant association of MMP-7 -181A/G (rs11568818) GG genotype with chronic pancreatitis patients, indicating its possible association with the disease.
authors.forename: K Sri
authors.initials: KS
authors.lastname: Manjari
authors.forename: A
authors.initials: A
authors.lastname: Jyothy
authors.forename: P Shravan
authors.initials: PS
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Prabhakar
authors.forename: Pratibha
authors.initials: P
authors.lastname: Nallari
authors.affiliation: Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Hyderabad, India.
authors.forename: A
authors.initials: A
authors.lastname: Venkateshwari
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D008875:Middle Aged; D050500:Pancreatitis, Chronic / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D020783:Matrix Metalloproteinase 7