title: SAffold viruses in pediatric patients with diarrhea in Thailand.
abstract: Saffold virus (SAFV) is a newly discovered human virus which is classified into the genus Cardiovirus of the family Picornaviridae. A total of 608 fecal specimens collected during January 2012 to December 2013 from children with diarrhea in Chiang Mai, Thailand were investigated for SAFV by RT-nested PCR and sequence analysis. Of these, nine out of 608 (1.5%) were positive for SAFVs and four genotypes were identified, SAFV1, SAFV2, SAFV3, and SAFV4. SAFV mono-infection was found in five cases (CMH-S038-12, CMH-S071-12, CMH-S102-12, CMH-N029-12, and CMH-S048-13), while co-infection with other viruses causing diarrhea was observed in four cases (CMH-S021-12, CMH-S115-12, CMH-N048-13 and CMH-N103-13). This study provides more information about the genetic background of SAFV circulating in pediatric patients with diarrhea in Thailand.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Arpaporn
authors.initials: A
authors.lastname: Yodmeeklin
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.forename: Watchaporn
authors.initials: W
authors.lastname: Chuchaona
authors.forename: Wilaiporn
authors.initials: W
authors.lastname: Saikruang
authors.forename: Rungnapa
authors.initials: R
authors.lastname: Malasao
authors.forename: Natthawan
authors.initials: N
authors.lastname: Chaimongkol
authors.forename: Tipachan
authors.initials: T
authors.lastname: Kongsricharoern
authors.forename: Nuthapong
authors.initials: N
authors.lastname: Ukarapol
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
mesh_terms: D000293:Adolescent; D018188:Cardiovirus Infections / Q000453:epidemiology* / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D016000:Cluster Analysis; D060085:Coinfection / Q000453:epidemiology / Q000821:virology; D003967:Diarrhea / Q000453:epidemiology* / Q000821:virology*; D005243:Feces / Q000821:virology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013785:Thailand / Q000453:epidemiology; D017945:Theilovirus / Q000302:isolation & purification*
keywords: Saffold virus; Thailand; children; diarrhea