title: High-throughput method for the determination of nitroimidazoles in muscle samples by liquid chromatography coupled to mass spectrometry.
abstract: We present a high-throughput confirmatory method for the analysis of 11 nitroimidazoles in muscle samples (metronidazole, MNZ; dimetridazole, DMZ; ronidazole, RNZ; tinidazole, TNZ; carnidazole, CRZ; secnidazole semihydrate, SCZ; ornidazole, ORZ; metronidazole-hydroxy, MNZ-OH; ipronidazole, IPZ; ipronidazole-hydroxy, IPZ-OH; and dimetridazole-hydroxy, HMMNI). Extraction with acetonitrile is efficient and simple, with the majority of recoveries ranging between 65 and 101%, and without the need for clean-up of the extracts. The chromatographic analysis is performed using UPLC-QqQ-MS in the MRM mode, with an electrospray source operated in positive mode. Total chromatographic analysis time is 12 min. This method has been fully validated in muscle according to Decision 657/2002/CE guidelines, and the CCα values obtained were in the range of 0.04-0.4 μg·kg(-1). The method is currently used for the analysis of muscle samples and has been tested in other matrices of animal origin, such as kidney, retina and egg, with excellent results.
authors.affiliation: Laboratori de l'Agència de Salut Pública de Barcelona, Avinguda Drassanes 13-15, 08001, Barcelona, Spain, arubies@aspb.cat.
authors.forename: A
authors.initials: A
authors.lastname: Rúbies
authors.forename: G
authors.initials: G
authors.lastname: Sans
authors.forename: P
authors.initials: P
authors.lastname: Kumar
authors.forename: M
authors.initials: M
authors.lastname: Granados
authors.forename: R
authors.initials: R
authors.lastname: Companyó
authors.forename: F
authors.initials: F
authors.lastname: Centrich
mesh_terms: D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D009132:Muscles / Q000737:chemistry; D009593:Nitroimidazoles / Q000032:analysis*; D011200:Poultry; D013552:Swine
chemical_list: D009593:Nitroimidazoles