title: Highly angiogenic peptide nanofibers.
abstract: Major limitations of current tissue regeneration approaches using artificial scaffolds are fibrous encapsulation, lack of host cellular infiltration, unwanted immune responses, surface degradation preceding biointegration, and artificial degradation byproducts. Specifically, for scaffolds larger than 200-500 μm, implants must be accompanied by host angiogenesis in order to provide adequate nutrient/waste exchange in the newly forming tissue. In the current work, we design a peptide-based self-assembling nanofibrous hydrogel containing cell-mediated degradation and proangiogenic moieties that specifically address these challenges. This hydrogel can be easily delivered by syringe, is rapidly infiltrated by cells of hematopoietic and mesenchymal origin, and rapidly forms an extremely robust mature vascular network. Scaffolds show no signs of fibrous encapsulation and after 3 weeks are resorbed into the native tissue. These supramolecular assemblies may prove a vital paradigm for tissue regeneration and specifically for ischemic tissue disease.
authors.affiliation: Department of Chemistry and Department of Bioengineering, Rice University Mail Stop 602, 6100 Main Street, Houston, Texas 77030, United States.
authors.forename: Vivek A
authors.initials: VA
authors.lastname: Kumar
authors.forename: Nichole L
authors.initials: NL
authors.lastname: Taylor
authors.forename: Siyu
authors.initials: S
authors.lastname: Shi
authors.forename: Benjamin K
authors.initials: BK
authors.lastname: Wang
authors.forename: Abhishek A
authors.initials: AA
authors.lastname: Jalan
authors.forename: Marci K
authors.initials: MK
authors.lastname: Kang
authors.forename: Navindee C
authors.initials: NC
authors.lastname: Wickremasinghe
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Hartgerink
mesh_terms: D000595:Amino Acid Sequence; D043925:Angiogenesis Inducing Agents / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D015195:Drug Design; D005260:Female; D020100:Hydrogels / Q000737:chemistry; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D057139:Nanofibers; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011487:Protein Conformation; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D043925:Angiogenesis Inducing Agents; D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides
keywords: angiogenesis; multidomain peptide; self-assembly; supramolecular chemistry