title: Genome-wide association study of antibody level response to NDV and IBV in Jinghai yellow chicken based on SLAF-seq technology.
abstract: Newcastle disease (ND) and avian infectious bronchitis (IB) are contagious diseases of chickens. To identify genes associated with antibody levels against ND and IB, a genome-wide association study was performed using specific-locus amplified fragment sequencing (SLAF-seq) technology in Jinghai yellow chickens. This determined six single-nucleotide polymorphisms (SNPs) that were associated with antibody levels against Newcastle disease virus (NDV): rsZ2494661, rsZ2494710, rs1211307701, rs1211307711, rs1218289310 and rs420701988. Of these, rsZ2494661 and rsZ2494710 reached the 5 % Bonferroni genome-wide significance level (5.5E-07) and they were both 134.7 kb downstream of the SETBP1 gene. The remaining four SNPs had 'suggestive' genome-wide significance levels (1.1E-05) and they were within or near the Plexin B1, LRRN1 and PDGFC genes. IB had two SNPs associated with antibody levels: rs149988433 and rs16170823; both reached chromosome-wide significance levels and they were near the USP7 and TRIM27 genes, respectively. Bioinformatics, GO annotation and pathway analysis indicated that five of these genes (Plexin B1, TRIM27, PDGFC, SETBP1 and USP7) may be important for the generation of protective antibodies against NDV and infectious bronchitis virus (IBV). This paves the way for further research on host immune responses against NDV.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
authors.forename: Wenhao
authors.initials: W
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Genxi
authors.initials: G
authors.lastname: Zhang
authors.forename: Jinyu
authors.initials: J
authors.lastname: Wang
authors.forename: Kunpeng
authors.initials: K
authors.lastname: Han
authors.forename: Yongjuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yinwen
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000917:Antibody Formation / Q000235:genetics*; D002645:Chickens / Q000235:genetics* / Q000276:immunology; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary*; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D060005:Genotyping Techniques; D001351:Infectious bronchitis virus; D009521:Newcastle Disease / Q000276:immunology*; D009522:Newcastle disease virus; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D000914:Antibodies, Viral; D005819:Genetic Markers