title: Metabolism of androstenone, 17β-estradiol and dihydrotestosterone in primary cultured pig hepatocytes and the role of 3β-hydroxysteroid dehydrogenase in this process.
abstract: Steroids metabolism plays an important role in mammals and contributes to quality of pig meat. The main objective of this study was to identify metabolites of androstenone, 17β-estradiol and dihydrotestosterone using primary cultured pig hepatocytes as a model system. The role of 3β-hydroxysteroid dehydrogenase (3βHSD) in regulation of steroid metabolism was also validated using trilostane, a specific 3βHSD inhibitor. Steroid glucuronide conjugated metabolites were detected by liquid chromatography time of flight mass spectrometry (LC-TOF-MS). 3βHSD enzyme was essential for metabolism of androstenone to 5α-androst-16-en-3β-ol, which then formed androstenone glucuronide conjugate. Metabolism of 17β-estradiol was accompanied by formation of glucuronide-conjugated estrone and glucuronide-conjugated estradiol. The ratio of the two metabolites was around 5:1. 3βHSD enzyme was not involved in 17β-estradiol metabolism. 5α-Dihydrotestosterone-17β-glucuronide was identified as a dihydrotestosterone metabolite, and this metabolism was related to 3βHSD enzyme activity as demonstrated by inhibition study.
authors.affiliation: Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Pathology, Air Force General Hospital, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Ren
authors.affiliation: Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, China Agricultural University, Beijing, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Fang
authors.affiliation: Centre for Research in Biosciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, United Kingdom.
authors.forename: Huda
authors.initials: H
authors.lastname: Al-Kateb
authors.affiliation: Centre for Research in Biosciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, United Kingdom.
authors.forename: Olena
authors.initials: O
authors.lastname: Doran
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D000736:Androstenes / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D013196:Dihydrotestosterone / Q000378:metabolism* / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism* / Q000494:pharmacology; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism*; D013552:Swine
chemical_list: D000736:Androstenes; D013196:Dihydrotestosterone; C000214:androst-16-en-3-one; D004958:Estradiol; D015096:3-Hydroxysteroid Dehydrogenases