title: Expression of ezrin in vagina cells of postmenopausal rats after dietary administration of omega-3 Fatty Acid formula.
abstract: OBJECTIVE
  To see the effect of dietary administration of omega 3-fatty acid formula on the vaginal cells of postmenopausal rats.
  
  
  METHODS
  Three-week-old female Wistar/ST rats were raised after one week of adjustment period. The rats were then divided into three groups, for three different kinds of diet; general diet, 1% omega-3 fatty acid diet, and 2% omega-3 fatty acid diet. After eight weeks of having assigned diet, after the oophorectomy, with the same diet previously they had Immunohistochemistry, Immunofluorescence, and Western Blot about ezrin, merlin were done.
  
  
  RESULTS
  In immunohistochemistry, estrogen injection group revealed thicker and well differentiated features. In Immunofluorescence, Omega-3 fatty acid composition in diet did not effect expression of ezrin and merlin in rat vagina in estrogen injection group, their vaginal epithelium showed full layers (from basal to apical layer). In Western Blot analysis, Omega-3 fatty acid composition in diet did not affect expression of ezrin and merlin in rat vagina estrogen presented significant impact on expression of ezrin and merlin.
  
  
  CONCLUSIONS
  Although omega-3 fatty acid composition changed in diet, vaginal epithelial morphology unchanged. Estrogen did effect on vagina cell, but omega-3 fatty acid did not effect on ezrin and merlin in vagina.
authors.affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea.
authors.forename: Hae-Hyeog
authors.initials: HH
authors.lastname: Lee
authors.affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea.
authors.forename: Junsik
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea. ; Department of Interdisciplinary Program in Biomedical Science, Soonchunhyang University, Asan, Korea.
authors.forename: Arum
authors.initials: A
authors.lastname: Lee
authors.affiliation: Department of Food and Nutrition, College of Human Ecology, Hanyang University, Seoul, Korea.
authors.forename: Yongsoon
authors.initials: Y
authors.lastname: Park
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, Soonchunhyang University College of Medicine, Seoul, Korea.
authors.forename: Dong Won
authors.initials: DW
authors.lastname: Byun
authors.affiliation: Department of Obstetrics and Gynecology, Soonchunhyang University College of Medicine, Bucheon, Korea. ; Department of Food and Nutrition, College of Human Ecology, Hanyang University, Seoul, Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Nutrition, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Heesook
authors.initials: H
authors.lastname: Lim
keywords: Atrophic vaginitis; Diet; Fatty acids omega-3; Vagina