title: Investigation of immunomodulatory properties of human Wharton's Jelly-derived mesenchymal stem cells after lentiviral transduction.
abstract: Human Wharton's Jelly-derived Mesenchymal Stem Cells (hWJ-MSCs) are considered as an alternative for bone-marrow-derived MSCs. These cells have immunosuppressive properties. It was unclear whether the WJ-MSCs would sustain their immunomodulatory characteristics after lentiviral transduction or not. In this study, we evaluated immunomodulatory properties of WJ-MSCs after lentiviral transduction. HWJ-MSCs were transduced with lentiviral particles. Expression of transduced and un-transduced hWJ-MSCs surface molecules and secretion of IL-10, HGF, VEGF and TGF-β was analyzed. Cell proliferation and frequency of CD4(+)CD25(+) CD127(low/neg) Foxp3(+) T regulatory cells was measured. There was no difference between the surface markers and secretion of IL-10, HGF, VEGF and TGF-β in transduced and un-transduced hWJ-MSCs. Both cells inhibited the proliferation of PHA stimulated PBMCs, and improved the frequency of T regulatory cells. These findings suggest that lentiviral transduction does not alter the immunomodulatory function of hWJ-MSCs. However, lentiviral transduction may have a wide range of applications in gene therapy.
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Amari
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: ebtekarm@modares.ac.ir.
authors.forename: Massoumeh
authors.initials: M
authors.lastname: Ebtekar
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Moazzeni
authors.affiliation: Hematology Department, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran; Stem Cells Biology Department, Stem Cell Technology Research Center, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Medical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
authors.forename: Leila Mohammadi
authors.initials: LM
authors.lastname: Amirabad
authors.affiliation: Department of Immunology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Taher
authors.initials: MT
authors.lastname: Tahoori
authors.affiliation: National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; Stem Cells Biology Department, Stem Cell Technology Research Center, Tehran, Iran. Electronic address: massumi.mohammad@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Massumi
mesh_terms: D002454:Cell Differentiation / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D017228:Hepatocyte Growth Factor / Q000032:analysis / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000235:genetics / Q000276:immunology*; D016753:Interleukin-10 / Q000032:analysis / Q000276:immunology; D016086:Lentivirus / Q000235:genetics; D007963:Leukocytes, Mononuclear; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000276:immunology*; D011247:Pregnancy; D014161:Transduction, Genetic; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000276:immunology; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis / Q000276:immunology; D059631:Wharton Jelly / Q000166:cytology* / Q000276:immunology
chemical_list: C066855:HGF protein, human; D007155:Immunologic Factors; D016212:Transforming Growth Factor beta; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D017228:Hepatocyte Growth Factor
keywords: Immunomodulatory properties; Lentiviral transduction; Wharton’s Jelly-derived Mesenchymal Stem Cells