title: New TiAg composite coating for bone prosthesis engineering shows promising microvascular compatibility in the murine dorsal skinfold chamber model.
abstract: The incorporation of antimicrobial substances like silver into implant surface coatings is one promising concept against primary infections of endoprosthesis, especially for immunocompromised patients as well as against reinfection after revision operations. However, besides good antimicrobial and mechanical properties it is equally important that the implant material does not disturb the local microvascular perfusion of muscle tissue to enable microbial host defense and tissue repair processes. In this study the biocompatibility of a newly developed TiAg-composite coating applied on conventional titanium via physical vapor deposition was analysed. To evaluate the local microvascular and inflammatory response of striated muscle tissue upon implantation of TiAg-coated plates the murine dorsal skinfold chamber model was used. We repetitively examined local capillary and venular perfusion, endothelial integrity as well as leucocyte activation by intravital fluorescence microscopy at 1 h, 24 h as well as 3 and 7 days after implantation. TiAg-implants were well tolerated by the vascular system as indicated by intact functional capillary density and endothelial integrity compared to pure titanium plates and controls without a metal implant. Furthermore, quantification of rolling and adherent leucocytes did not reveal signs of inflammation upon TiAg-implantation.
authors.affiliation: Department of Trauma, Hand and Reconstructive Surgery, Rostock University Medical Center, Rostock, Germany, behrendt.ak@googlemail.com.
authors.forename: Ann-Kathrin
authors.initials: AK
authors.lastname: Behrendt
authors.forename: Maximilian
authors.initials: M
authors.lastname: Beythien
authors.forename: Jakob
authors.initials: J
authors.lastname: Huber
authors.forename: Thorsten
authors.initials: T
authors.lastname: Zufraß
authors.forename: Antje
authors.initials: A
authors.lastname: Butschkau
authors.forename: Thomas
authors.initials: T
authors.lastname: Mittlmeier
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vollmar
mesh_terms: D000535:Aluminum / Q000737:chemistry; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000473:pathology*; D002196:Capillaries / Q000378:metabolism; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005260:Female; D006439:Hemodynamics; D007249:Inflammation; D036904:Leukocyte Rolling; D007962:Leukocytes / Q000166:cytology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D010477:Perfusion; D012834:Silver / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D012834:Silver; D000535:Aluminum; D014025:Titanium