title: Relationship between physicochemical properties and enzymatic hydrolysis of sugarcane bagasse varieties for bioethanol production.
abstract: The structural and physicochemical characteristics are associated with resistance of plant cell walls to saccharification by enzymes. The effect of physicochemical properties on glucose yield of bagasse from different varieties of sugarcane at low and high enzyme dosages was investigated. The result showed that glucose yield at low enzyme dosage was positively linear correlated with the yield at high enzyme dosage, for both the untreated and pretreated materials. The pretreatment significantly increased the accessibility of substrates by enzyme due to the increase of internal and external surface area. Glucose yield also showed a linear correlation with dye adsorption. However, the increase in glucose yield as a result of pretreatment did not correlate with the increases in crystallinity index and decreases in degree of polymerization. The Principal Component Analysis of infrared data indicated that lignin was the main component that differentiated the varieties before and after pretreatment. These results suggested that the key differences in pretreatment responses among varieties could be mainly attributed to their differences in the internal and external surface area after pretreatment.
authors.affiliation: Department of Process Engineering, University of Stellenbosch - Private Bag X1, Stellenbosch 7602, South Africa. Electronic address: michelbrienzo@yahoo.com.br.
authors.forename: Michel
authors.initials: M
authors.lastname: Brienzo
authors.affiliation: Department of Forest and Wood Science, University of Stellenbosch - Private Bag X1, Stellenbosch 7602, South Africa.
authors.forename: Luvuyo
authors.initials: L
authors.lastname: Tyhoda
authors.affiliation: Department of Process Engineering, University of Stellenbosch - Private Bag X1, Stellenbosch 7602, South Africa.
authors.forename: Yuda
authors.initials: Y
authors.lastname: Benjamin
authors.affiliation: Department of Process Engineering, University of Stellenbosch - Private Bag X1, Stellenbosch 7602, South Africa.
authors.forename: Johann
authors.initials: J
authors.lastname: Görgens
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D055598:Chemical Phenomena; D003460:Crystallization; D000431:Ethanol / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis / Q000187:drug effects; D058105:Polymerization; D031786:Saccharum / Q000737:chemistry* / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D013464:Sulfuric Acids / Q000494:pharmacology; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D056804:Biofuels; D013464:Sulfuric Acids; D000431:Ethanol; D002482:Cellulose; C027433:bagasse; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; C033158:sulfuric acid