title: DNA damage in grasshopper Chorthippus brunneus (Orthoptera) hatchlings following paraquat exposure.
abstract: Comet assay was applied to study genotoxic damage induced by paraquat (PQ) in brain cells of Chorthippus brunneus (Insecta: Orthoptera) hatchlings. Percentage of the comet fluorescence in the tail (TDNA), length of the comet tail (TL) and Olive tail moment (OTM) were used for quantitative assessment of the DNA damage. Multiple regression analysis supplemented standard statistical elaboration of the results. Increasing PQ concentrations applied either directly to the brain cells suspension (10, 50, and 250 μM PQ final concentration--in vitro protocol) or indirectly (50, 250, and 1250 μM PQ final concentration--in vivo protocol) provoked significant increase of oxidative damage to DNA (higher median TDNA and OTM values). The damage increased with time of exposure (0, 5, 15, and 30 min) following in vitro application, but decreased in longer interval (3 vs 24 h) after in vivo administration of paraquat. On contrary, median TL values did not correlate with paraquat concentration irrespectively of the exposure protocol. Possible reason of this discrepancy in light of paraquat toxicity is discussed.
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Augustyniak
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: Ł
authors.initials: Ł
authors.lastname: Nocoń
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland. Electronic address: andrzej.kedziorski@us.edu.pl.
authors.forename: A
authors.initials: A
authors.lastname: Kędziorski
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Łaszczyca
authors.affiliation: Department of Physiology in Zabrze, Medical University of Silesia, Jordana 19, PL 41-808 Zabrze, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Sawczyn
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Tarnawska
authors.affiliation: Department of Animal Physiology and Ecotoxicology, University of Silesia, Bankowa 9, PL 40-007 Katowice, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Zawisza-Raszka
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D020552:Comet Assay; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006110:Grasshoppers / Q000187:drug effects* / Q000235:genetics; D006540:Herbicides / Q000633:toxicity*; D010269:Paraquat / Q000633:toxicity*; D012044:Regression Analysis; D013997:Time Factors
chemical_list: D006540:Herbicides; D004247:DNA; D010269:Paraquat
keywords: Comet assay; DNA damage; Grasshoppers; In vitro; In vivo; Paraquat