title: Expression of CD44 and CD35 during normal and myelodysplastic erythropoiesis.
abstract: Erythroid dysplasia is a common feature of myelodysplastic syndromes (MDS). Currently available information about the immunophenotypic features of normal and dysplastic erythropoiesis is scarce and restricted to relatively few markers. Here we studied the expression of CD117, CD35 and CD44 throughout the normal (n=16) and dysplastic (n=48) bone marrow erythroid maturation. CD35 emerged as an early marker of CD34(+) erythroid-committed precursors, which is expressed before CD105 and remains positive thereafter. MDS patients (with and without morphologic dyserythropoiesis) displayed overall increased expression of CD44, associated with slight alterations on CD35 expression, suggesting that phenotypic alterations in MDS may precede morphologic dysplasia. In turn, MDS patients with anemia showed increased expression of CD117.
authors.affiliation: Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal. Electronic address: 1979paula@gmail.com.
authors.forename: Paula
authors.initials: P
authors.lastname: Laranjeira
authors.affiliation: College of Health Technology of Coimbra, Rua 5 de Outubro, 3046-854 Coimbra, Portugal; Centro Hospitalar Tondela-Viseu, EPE, Av. Rei D. Duarte, 2504-509 Viseu, Portugal. Electronic address: raquel.sl.rodrigues@gmail.com.
authors.forename: Raquel
authors.initials: R
authors.lastname: Rodrigues
authors.affiliation: Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal. Electronic address: tiago.carvalheiro@gmail.com.
authors.forename: Tiago
authors.initials: T
authors.lastname: Carvalheiro
authors.affiliation: Centro Hospitalar Tondela-Viseu, EPE, Av. Rei D. Duarte, 2504-509 Viseu, Portugal. Electronic address: saoconstanco@hotmail.com.
authors.forename: Conceição
authors.initials: C
authors.lastname: Constanço
authors.affiliation: Centro Hospitalar Tondela-Viseu, EPE, Av. Rei D. Duarte, 2504-509 Viseu, Portugal. Electronic address: hvitoria@sapo.pt.
authors.forename: Helena
authors.initials: H
authors.lastname: Vitória
authors.affiliation: Centro de Investigación del Cáncer (Instituto de Biología Molecular y Celular del Cáncer; CSIC-USAL), Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain. Electronic address: smats@usal.es.
authors.forename: Sergio
authors.initials: S
authors.lastname: Matarraz
authors.affiliation: Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal. Electronic address: helder.trindade@ipst.min-saude.pt.
authors.forename: Hélder
authors.initials: H
authors.lastname: Trindade
authors.affiliation: Centro de Investigación del Cáncer (Instituto de Biología Molecular y Celular del Cáncer; CSIC-USAL), Universidad de Salamanca, Campus Miguel de Unamuno, 37007 Salamanca, Spain. Electronic address: orfao@usal.es.
authors.forename: Alberto
authors.initials: A
authors.lastname: Órfão
authors.affiliation: Blood and Transplantation Center of Coimbra, Portuguese Institute of the Blood and Transplantation, Quinta da Vinha Moura, São Martinho do Bispo, 3041-861 Coimbra, Portugal; College of Health Technology of Coimbra, Rua 5 de Outubro, 3046-854 Coimbra, Portugal. Electronic address: artur.paiva@ipst.min-saude.pt.
authors.forename: Artur
authors.initials: A
authors.lastname: Paiva
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D015672:Erythroid Precursor Cells / Q000378:metabolism* / Q000473:pathology; D004920:Erythropoiesis; D005260:Female; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D016130:Immunophenotyping; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000378:metabolism* / Q000473:pathology; D009367:Neoplasm Staging; D010641:Phenotype; D011379:Prognosis; D017463:Receptors, Complement 3b / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C497483:CD44 protein, human; C509991:CR1 protein, human; D018960:Hyaluronan Receptors; D017463:Receptors, Complement 3b
keywords: CD117; CD35; CD44; Erythroid maturation; Erythroid precursor; Myelodysplastic syndrome