title: Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry.
abstract: Glioblastoma is the most common brain tumor and is characterized with robust invasion and migration potential resulting in poor prognosis. Previous investigations have demonstrated that modeled microgravity (MMG) could decline the cell proliferation and attenuate the metastasis potential in several cell lines. In this study, we studied the effects of MMG on the invasion and migration potentials of glioblastoma in human glioblastoma U87 cells. We found that MMG stimulation significantly attenuated the invasion and migration potentials, decreased thapsigargin (TG) induced store-operated calcium entry (SOCE) and downregulated the expression of Orai1 in U87 cells. Inhibition of SOCE by 2-APB or stromal interaction molecule 1 (STIM1) downregulation both mimicked the effects of MMG on the invasion and migration potentials in U87 cells. Furthermore, upregulation of Orai1 significantly weakened the effects of MMG on the invasion and migration potentials in U87 cells. Therefore, these findings indicated that MMG stimulation inhibited the invasion and migration potentials of U87 cells by downregulating the expression of Orai1 and sequentially decreasing the SOCE, suggesting that MMG might be a new potential therapeutic strategy in glioblastoma treatment in the future.
authors.affiliation: Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Zi-xuan
authors.initials: ZX
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Rao
authors.affiliation: Department of Dermatology, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xi'an Traditional Chinese Medicine Hospital, Xi'an, 710032, PR China.
authors.forename: Nan-ding
authors.initials: ND
authors.lastname: Wang
authors.affiliation: Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Jing-wen
authors.initials: JW
authors.lastname: Si
authors.affiliation: Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China.
authors.forename: Jun-chang
authors.initials: JC
authors.lastname: Li
authors.affiliation: Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, PR China. Electronic address: zongren@fmmu.edu.cn.
authors.forename: Zong-ren
authors.initials: ZR
authors.lastname: Wang
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology / Q000628:therapy*; D006801:Humans; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics; D000071740:ORAI1 Protein; D034622:RNA Interference; D000071737:Stromal Interaction Molecule 1; D015854:Up-Regulation; D018474:Weightlessness Simulation
chemical_list: D015220:Calcium Channels; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000071740:ORAI1 Protein; C510746:ORAI1 protein, human; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1; D002118:Calcium
keywords: Glioblastoma; Invasion; Microgravity; Migration; SOCE