title: Lipid production and mixotrophic growth features of cyanobacterial strains isolated from various aquatic sites.
abstract: The present study was conducted to determine the potential of five cyanobacteria strains isolated from aquatic zones to induce lipid production. The phylogenetic affiliation of the isolates was determined by 16S rRNA gene sequencing. Amongst the isolates, an efficient cyanobacterium, Synechococcus sp. HS01 showing maximal biomass and lipid productivity, was selected for further studies. In order to compare lipid productivity, the HS01 strain was grown in different media to screen potential significant culture ingredients and to evaluate mixotrophic cultivation. Mixotrophic cultivation of the strain using ostrich oil as a carbon source resulted in the best lipid productivity. GC analysis of fatty acid methyl esters of the selected cyanobacterial strain grown in media supplemented with ostrich oil showed a high content of C16 (palmitoleic acid and palmitic acid) and C18 (linoleic acid, oleic acid and linolenic acid) fatty acids of 42.7 and 42.8 %, respectively. Transmission electron micrographs showed that the HS01 cells exhibited an elongated rod-shaped appearance, either isolated, paired, linearly connected or in small clusters. According to initial experiments, ostrich oil, NaNO3 and NaCl were recognized as potential essential nutrients and selected for optimization of media with the goal of maximizing lipid productivity. A culture optimization technique using the response surface method demonstrated a maximum lipid productivity of 56.5 mg l(-1) day(-1). This value was 2.82-fold higher than that for the control, and was achieved in medium containing 1.12 g l(-1) NaNO3, 1 % (v/v) ostrich oil and 0.09 % (w/v) NaCl.
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Modiri
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran Department of Microbiology, Faculty of Sciences, Alzahra University, Tehran, Iran.
authors.forename: Hakimeh
authors.initials: H
authors.lastname: Sharafi
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Alidoust
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Hajfarajollah
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Haghighi
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Aisan
authors.initials: A
authors.lastname: Azarivand
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Zamanzadeh
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran.
authors.forename: Hossein Shahbani
authors.initials: HS
authors.lastname: Zahiri
authors.affiliation: Facility for Electron Microscopy Research, McGill University, 3640 Street, Montreal, Quebec, Canada.
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
authors.affiliation: Division of Industrial & Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), PO Box 14155-6343, Tehran, Iran Akbari@nigeb.ac.ir.
authors.forename: Kambiz Akbari
authors.initials: KA
authors.lastname: Noghabi
mesh_terms: D060106:Lakes / Q000382:microbiology*; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D010802:Phylogeny; D046940:Synechococcus / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D008055:Lipids