title: Influence of 3D porous galactose containing PVA/gelatin hydrogel scaffolds on three-dimensional spheroidal morphology of hepatocytes.
abstract: Three-dimensional liver scaffolds are temporary framework that mimics native ECM architecture and positively influence hepatocyte lodging, proliferation with retention of metabolic activities. The aim of the current study is to develop galactose containing physical cross-linked polyvinyl alcohol/gelatin (P/G 8:2 and 9:1) hydrogel scaffolds via freeze/thaw technique. The 8:2 and 9:1 P/G hydrogels exhibited comparable pore size and porosity (P > 0.05). The tensile strength of the fabricated 8:2 and 9:1 P/G hydrogel scaffolds were found to be in accordance with native human liver. Pore interconnectivity of both the P/G hydrogel scaffolds was confirmed by scanning electron micrographs and liquid displacement method. Further galactose containing hydrogel promoted cell-cell and cell-hydrogel interaction, aiding cellular aggregation leading to spheroids formation compared to void P/G hydrogel by 7 days. Hence, galactose containing P/G hydrogel could be more promising substrate as it showed significantly higher cell proliferation and albumin secretion for 21 days when compared to non-galactose P/G hydrogels (P < 0.05).
authors.affiliation: Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical & Biotechnology, SASTRA University, Thanjavur, 613 401, Tamil Nadu, India.
authors.forename: Kirthanashri S
authors.initials: KS
authors.lastname: Vasanthan
authors.forename: Anuradha
authors.initials: A
authors.lastname: Subramaniam
authors.forename: Uma Maheswari
authors.initials: UM
authors.lastname: Krishnan
authors.forename: Swaminathan
authors.initials: S
authors.lastname: Sethuraman
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002448:Cell Adhesion; D019070:Cell Lineage; D049109:Cell Proliferation; D002470:Cell Survival; D003432:Cross-Linking Reagents / Q000737:chemistry; D004058:Diffusion; D005109:Extracellular Matrix / Q000378:metabolism; D005690:Galactose / Q000737:chemistry*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008855:Microscopy, Electron, Scanning; D011145:Polyvinyls / Q000737:chemistry*; D016062:Porosity; D012038:Regeneration; D013696:Temperature; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000418:Albumins; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D020100:Hydrogels; D011145:Polyvinyls; C013215:polyvinyl acetate; D005690:Galactose