title: Accumulation of 24S-hydroxycholesterol in neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0): argument in favor of 24S-hydroxycholesterol as a potential biomarker of neurolipotoxicity.
abstract: Cholesterol oxide derivatives (oxysterols) are viewed as potential biomarkers of neurodegenerative diseases. 24S-hydroxycholesterol, an oxysterol produced only in brain neurons, is often found for unknown reasons in increased levels in the plasma in patients with neurodegenerative diseases. On human neuronal SK-N-BE cells treated with hexacosanoic acid (C26:0) identified at increased levels in the tissues and plasma of patients with peroxisomal leukodystrophies and Alzheimer's disease, we observed increased level of 24S-hydroxycholesterol associated with C26:0 induced lipotoxicity. This finding reinforces the hypothesis suggesting that 24S-hydroxycholesterol could constitute a biomarker of neurotoxicity.
authors.affiliation: Team 'Biochemistry of Peroxisome, Inflammation and Lipid Metabolism', EA 7270, INSERM, faculté des sciences Gabriel, University of Bourgogne, 6, boulevard Gabriel, 21000 Dijon, France; LR12ES05, Lab-NAFS 'Nutrition-Functional Food and Vascular Health', faculté de médecine, University of Monastir, avenue Avicenne, Monastir, Tunisia.
authors.forename: A
authors.initials: A
authors.lastname: Zarrouk
authors.affiliation: LR12ES05, Lab-NAFS 'Nutrition-Functional Food and Vascular Health', faculté de médecine, University of Monastir, avenue Avicenne, Monastir, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Hammami
authors.affiliation: Department of Neurology, University Hospital of Dijon, Bocage central, 14, rue Gaffarel, BP 77908, 21079 Dijon cedex, France.
authors.forename: T
authors.initials: T
authors.lastname: Moreau
authors.affiliation: Team 'Biochemistry of Peroxisome, Inflammation and Lipid Metabolism', EA 7270, INSERM, faculté des sciences Gabriel, University of Bourgogne, 6, boulevard Gabriel, 21000 Dijon, France. Electronic address: gerard.lizard@u-bourgogne.fr.
authors.forename: G
authors.initials: G
authors.lastname: Lizard
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D002784:Cholesterol / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D006801:Humans; D006888:Hydroxycholesterols / Q000378:metabolism*; D008055:Lipids / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D020258:Neurotoxicity Syndromes / Q000175:diagnosis* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D006888:Hydroxycholesterols; D008055:Lipids; C044563:24-hydroxycholesterol; D002784:Cholesterol; C017364:hexacosanoic acid
keywords: 24S-hydroxycholesterol; 24S-hydroxycholestérol; Acide hexacosanoïque (C26:0); Hexacosanoic acid (C26:0); Lipotoxicity; Lipotoxicité; Neuronal stress; Stress neuronal