title: Occurrence, molecular characterization, and antimicrobial susceptibility of Aeromonas spp. in marine species of shrimps cultured at inland low salinity ponds.
abstract: We aimed to document the risk of Aeromonas spp. in marine shrimp species cultured in inland low salinity ponds in Thailand. In 14 of 18 shrimp samples retrieved from inland grow-up ponds, Aeromonas spp. were detected at ranges from 4667 to 1,500,000 CFU/g body weight. The phylogenetic tree constructed with the gyrB and cpn60 concatenated sequences indicated that the 87 isolates consisted of Aeromonas veronii (70%), Aeromonas aquariorum (18%), Aeromonas caviae (7%), Aeromonas jandaei (2%), and Aeromonas schubertii (2%). The potential virulence of the isolates was examined by phenotypic and PCR assays. Hemolytic activity and the extracellular activity of lipase, DNase, and gelatinase were observed in most isolates (94-99%). PCR revealed the presence of 9 genes related to virulence in the 87 isolates: act (75%), aer (74%), alt (30%), ast (1%), ascV (34%), aexT (24%), fla (92%), ela (34%), and lip (24%). The susceptibility profiles to 14 antimicrobial agents of isolates were typical for the genus, but resistance to cefotaxime, a third-generation cephalosporin, and imipenem were found in two A. aquariorum and in three A. veronii isolates, respectively. These resistances were confirmed by determining minimum inhibitory concentrations. Our results indicate that the microbiological risk posed by Aeromonas should be considered for marine shrimp species that are cultured in low-salinity ponds. These shrimps may also be a vehicle for the transfer of different genotypes of Aeromonas and antibiotic-resistant determinants to regions worldwide through trade.
authors.affiliation: Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Sapporo, Japan. Electronic address: yanoya@affrc.go.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yano
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Onomichi, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Hamano
authors.affiliation: Japan International Research Center for Agricultural Sciences, Tsukuba, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Tsutsui
authors.affiliation: Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
authors.forename: Dusit
authors.initials: D
authors.lastname: Aue-Umneoy
authors.affiliation: Hokkaido National Fisheries Research Institute, Fisheries Research Agency, Sapporo, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Ban
authors.affiliation: National Research Institute of Fisheries Science, Fisheries Research Agency, Yokohama, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Satomi
mesh_terms: D000333:Aeromonas / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000378:metabolism; D004352:Drug Resistance, Microbial; D018093:Gelatinases / Q000378:metabolism; D006461:Hemolysis; D015378:Imipenem / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D033561:Penaeidae / Q000382:microbiology*; D010399:Penicillin Amidase / Q000494:pharmacology; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D054712:Salinity; D012758:Shellfish / Q000382:microbiology*; D013785:Thailand; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D003851:Deoxyribonucleases; D018093:Gelatinases; D010399:Penicillin Amidase; D002439:Cefotaxime
keywords: Aeromonas spp; Antibiotic resistance; Inland pond culture; Marine shrimp; Virulence factor