title: Genome sequence of Coxiella burnetii strain Namibia.
abstract: We present the whole genome sequence and annotation of the Coxiella burnetii strain Namibia. This strain was isolated from an aborting goat in 1991 in Windhoek, Namibia. The plasmid type QpRS was confirmed in our work. Further genomic typing placed the strain into a unique genomic group. The genome sequence is 2,101,438 bp long and contains 1,979 protein-coding and 51 RNA genes, including one rRNA operon. To overcome the poor yield from cell culture systems, an additional DNA enrichment with whole genome amplification (WGA) methods was applied. We describe a bioinformatics pipeline for improved genome assembly including several filters with a special focus on WGA characteristics.
authors.affiliation: Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany.|Department of Genome-Oriented Bioinformatics, Center of Life and Food Science Weihenstephan, Technische Universität München, Freising, Germany.
authors.forename: Mathias C
authors.initials: MC
authors.lastname: Walter
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Caroline
authors.initials: C
authors.lastname: Öhrman
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Myrtennäs
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Sjödin
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Mona
authors.initials: M
authors.lastname: Byström
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Pär
authors.initials: P
authors.lastname: Larsson
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Macellaro
authors.affiliation: CBRN Defence and Security, Swedish Defence Research Agency (FOI), Umeå, Sweden.
authors.forename: Mats
authors.initials: M
authors.lastname: Forsman
authors.affiliation: Bundeswehr Institute of Microbiology (BwIM), Munich, Germany.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Frangoulidis
keywords: Annotation; Assembly; Coxiella burnetii; Next generation sequencing (NGS); Q fever; Whole genome amplification; Whole genome sequencing