title: Golgi/plastid-type manganese superoxide dismutase involved in heat-stress tolerance during grain filling of rice.
abstract: Superoxide dismutase (SOD) is widely assumed to play a role in the detoxification of reactive oxygen species caused by environmental stresses. We found a characteristic expression of manganese SOD 1 (MSD1) in a heat-stress-tolerant cultivar of rice (Oryza sativa). The deduced amino acid sequence contains a signal sequence and an N-glycosylation site. Confocal imaging analysis of rice and onion cells transiently expressing MSD1-YFP showed MSD1-YFP in the Golgi apparatus and plastids, indicating that MSD1 is a unique Golgi/plastid-type SOD. To evaluate the involvement of MSD1 in heat-stress tolerance, we generated transgenic rice plants with either constitutive high expression or suppression of MSD1. The grain quality of rice with constitutive high expression of MSD1 grown at 33/28 °C, 12/12 h, was significantly better than that of the wild type. In contrast, MSD1-knock-down rice was markedly susceptible to heat stress. Quantitative shotgun proteomic analysis indicated that the overexpression of MSD1 up-regulated reactive oxygen scavenging, chaperone and quality control systems in rice grains under heat stress. We propose that the Golgi/plastid MSD1 plays an important role in adaptation to heat stress.
authors.affiliation: Department of Applied Biological Chemistry, Niigata University, Niigata, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Shiraya
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Mori
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Maruyama
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Takamatsu
authors.affiliation: Department of Applied Biological Chemistry, Niigata University, Niigata, Japan.
authors.forename: Kazusato
authors.initials: K
authors.lastname: Oikawa
authors.affiliation: Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Kimiko
authors.initials: K
authors.lastname: Itoh
authors.affiliation: Department of Applied Biological Chemistry, Niigata University, Niigata, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kaneko
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Ichikawa
authors.affiliation: Department of Applied Biological Chemistry, Niigata University, Niigata, Japan.|Graduate School of Science and Technology, Niigata University, Niigata, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui
mesh_terms: D000595:Amino Acid Sequence; D055785:Gene Knockdown Techniques; D006056:Golgi Apparatus / Q000201:enzymology*; D018869:Heat-Shock Response / Q000502:physiology*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000502:physiology; D018087:Plastids / Q000201:enzymology*; D012639:Seeds / Q000254:growth & development; D013482:Superoxide Dismutase / Q000235:genetics / Q000502:physiology*
chemical_list: D013482:Superoxide Dismutase
keywords: Golgi; Oryza sativa L.; grain quality; high-temperature tolerance; plastid; superoxide dismutase