title: Acid stability of the kinetically stable alkaline serine protease possessing polyproline II fold.
abstract: The kinetically stable alkaline serine protease from Nocardiopsis sp.; NprotI, possessing polyproline II fold (PPII) was characterized for its pH stability using proteolytic assay, fluorescence and Circular Dichroism (CD) spectroscopy, and Differential Scanning Calorimetry (DSC). NprotI was found to be functionally stable when incubated at pH 1.0, even after 24 h, while after incubation at pH 10.0, drastic loss in the activity was observed. The enzyme showed enhanced activity after incubation at pH 1.0 and 3.0, at higher temperature (50-60 °C). NprotI maintained the overall PPII fold in broad pH range as seen using far UV CD spectroscopy. The PPII fold of NprotI incubated at pH 1.0 remained fairly intact up to 70 °C. Based on the isodichroic point and Tm values revealed by secondary structural transitions, different modes of thermal denaturation at pH 1.0, 5.0 and 10.0 were observed. DSC studies of NprotI incubated at acidic pH (pH 1.0-5.0) showed Tm values in the range of 74-76 °C while significant decrease in Tm (63.8 °C) was observed at pH 10.0. NprotI could be chemically denatured at pH 5.0 (stability pH) only with guanidine thiocynate. NprotI can be classified as type III protein among the three acid denatured states. Acid tolerant and thermostable NprotI can serve as a potential candidate for biotechnological applications.
authors.affiliation: Division of Biochemical Sciences, National Chemical Laboratory, Dr. HomoBhabha Road, Pune, 411008, India.
authors.forename: Sonali
authors.initials: S
authors.lastname: Rohamare
authors.forename: Vaishali
authors.initials: V
authors.lastname: Javdekar
authors.forename: Sayli
authors.initials: S
authors.lastname: Dalal
authors.forename: Pavan Kumar
authors.initials: PK
authors.lastname: Nareddy
authors.forename: Musti J
authors.initials: MJ
authors.lastname: Swamy
authors.forename: Sushama M
authors.initials: SM
authors.lastname: Gaikwad
mesh_terms: D039903:Actinobacteria / Q000201:enzymology*; D001426:Bacterial Proteins / Q000737:chemistry*; D002942:Circular Dichroism; D004795:Enzyme Stability; D008956:Models, Chemical; D010455:Peptides / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012697:Serine Endopeptidases / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; C011083:polyproline; D012697:Serine Endopeptidases