title: Measurement of a urinary marker (8-hydroxydeoxy-guanosine, 8-OHdG) of DNA oxidative stress in epidemiological surveys: a pilot study.
abstract: BACKGROUND
  8-Hydroxydeoxyguanosine (8-OHdG) is a commonly used marker of DNA oxidative stress in epidemiological studies. The aim of this study was to establish whether the urinary concentration of 8-OHdG varies during the first part of the day, when clinical tests are usually performed, and whether it can therefore be measured without bias in spot urine samples.
  
  
  METHODS
  Spot urine samples were collected using a convenience sample. A linear mixed-effects model for repeated measurements was used to analyze 8-OHdG levels.
  
  
  RESULTS
  A significant increasing trend in time in the 8-OHdG concentration was found among smokers, but not in the case of nonsmokers.
  
  
  CONCLUSIONS
  In epidemiological studies on oxidative stress, all participants should collect their early morning urine specimens - before their first cigarette if they are smokers - to gather information on individual background oxidation levels.
authors.affiliation: Unit of Epidemiology and Medical Statistics, University of Verona, Verona - Italy.
authors.forename: M Elisabetta
authors.initials: ME
authors.lastname: Zanolin
authors.forename: Paolo
authors.initials: P
authors.lastname: Girardi
authors.forename: Paolo
authors.initials: P
authors.lastname: Degan
authors.forename: Marta
authors.initials: M
authors.lastname: Rava
authors.forename: Mario
authors.initials: M
authors.lastname: Olivieri
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Di Gennaro
authors.forename: Morena
authors.initials: M
authors.lastname: Nicolis
authors.forename: Roberto
authors.initials: R
authors.lastname: De Marco
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers; D015992:Body Mass Index; D002940:Circadian Rhythm; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D004032:Diet; D005260:Female; D005638:Fruit; D006184:Habits; D006801:Humans; D008297:Male; D008875:Middle Aged; D009043:Motor Activity; D018384:Oxidative Stress; D010865:Pilot Projects; D012907:Smoking / Q000453:epidemiology / Q000652:urine; D011795:Surveys and Questionnaires; D014675:Vegetables; D055815:Young Adult
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine