title: Enhanced hydrolysis of Macrocystis pyrifera by integrated hydroxyl radicals and hot water pretreatment.
abstract: Integrated hydroxyl radicals and hot water pretreatment (IHRHW) was employed in the bioconversion of the brown macroalgae Macrocystis pyrifera (M. pyrifera) in this study. The optimum experimental pretreatment condition (100°C, 30 min, 11.9 mM FeSO4) and the predicted optimum pretreatment condition (113.95°C, 29.1 min, 12.75 mM FeSO4) were identified using a central composite design method. All glucan and xylan were recovered as monosaccharides or polysaccharides without a fermentation inhibitor (e.g., hydroxymethyl furfural and furfural). The IHRHW-treated macroalgae digestibility reached 88.1% under the optimum experimental condition, whereas that under the predicted optimum condition reached 92.1%. The value was approximately threefold higher than those obtained with untreated M. pyrifera. Carbohydrate recovery and enzymatic hydrolysis can be significantly enhanced by the new economic hydroxyl radicals and hot water pretreatment.
authors.affiliation: Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Le
authors.initials: L
authors.lastname: Gao
authors.affiliation: Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Dongyuan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory for Sustainable Utilization of Marine Fishery Resources, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
authors.forename: Naihao
authors.initials: N
authors.lastname: Ye
authors.affiliation: Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
authors.affiliation: Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. Electronic address: demaoli@gmail.com.
authors.forename: Demao
authors.initials: D
authors.lastname: Li
mesh_terms: D001709:Biotechnology / Q000379:methods*; D005240:Feasibility Studies; D005285:Fermentation / Q000187:drug effects; D005296:Ferrous Compounds / Q000494:pharmacology; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D006868:Hydrolysis; D017665:Hydroxyl Radical / Q000494:pharmacology*; D044662:Macrocystis / Q000187:drug effects / Q000378:metabolism*; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014867:Water / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D005296:Ferrous Compounds; D014867:Water; D017665:Hydroxyl Radical; C020748:ferrous sulfate; D005947:Glucose
keywords: Fenton system; Hydroxyl radicals; Integrated hydroxyl radicals and hot water pretreatment; Macroalgae; Pretreatment