title: HPLC and LC-MS/MS methods for determination of sodium benzoate and potassium sorbate in food and beverages: performances of local accredited laboratories via proficiency tests in Turkey.
abstract: High Performance Liquid Chromatography LC-UV and LC-MS/MS methods were developed and validated for quantitative analyses of sodium benzoate and potassium sorbate in foods and beverages. HPLC-UV and LC-MS/MS methods were compared for quantitative analyses of sodium benzoate and potassium sorbate in a representative ketchup sample. Optimisation of the methods enabled the chromatographic separation of the analytes in less than 4 min. A correlation coefficient of 0.999 was achieved over the measured calibration range for both compounds and methods (HPLC and LC-MS/MS). The uncertainty values of sodium benzoate and potassium sorbate were found as 0.199 and 0.150 mg/L by HPLC and 0.072 and 0.044 mg/L by LC-MS/MS, respectively. Proficiency testing performance of Turkish accredited laboratories between the years 2005 and 2013 was evaluated and reported herein. The aim of the proficiency testing scheme was to evaluate the performance of the laboratories, analysing benzoate and sorbate in tomato ketchup.
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey. Electronic address: ahmetceyhan.goren@tubitak.gov.tr.
authors.forename: Ahmet C
authors.initials: AC
authors.lastname: Gören
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Gökhan
authors.initials: G
authors.lastname: Bilsel
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Şimşek
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Bilsel
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Akçadağ
authors.affiliation: TUBITAK UME (National Metrology Institute), Group of Chemistry, P. O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Kevser
authors.initials: K
authors.lastname: Topal
authors.affiliation: Zivak Technologies, Gürsel District, Old Beşiktaş Street, No:44/4, 34400 Kağıthane, İstanbul, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Ozgen
mesh_terms: D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005520:Food Preservatives / Q000737:chemistry*; D020160:Sodium Benzoate / Q000032:analysis* / Q000737:chemistry; D013011:Sorbic Acid / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014421:Turkey
chemical_list: D005520:Food Preservatives; D020160:Sodium Benzoate; D013011:Sorbic Acid
keywords: Food additives; HPLC–UV; Ketchup; LC–MS/MS; Method validation; Orange juice; Potassium sorbate; Proficiency testing; Sodium benzoate; Uncertainty budget