title: Syndecan-4 is a major syndecan in primary human endothelial cells in vitro, modulated by inflammatory stimuli and involved in wound healing.
abstract: Syndecans are important cell surface proteoglycans with many functions; yet, they have not been studied to a very large extent in primary human endothelial cells. The purpose of this study was to investigate syndecan-4 expression in cultured human umbilical vein endothelial cells (HUVECs) and assess its role in inflammatory reactions and experimental wound healing. qRT-PCR analysis revealed that syndecan-3 and syndecan-4 were highly expressed in HUVECs, whereas the expression of syndecan-1 and -2 was low. HUVECs were cultured with the inflammatory mediators lipopolysaccharide (LPS) and interleukin 1β (IL-1β). As a result, syndecan-4 expression showed a rapid and strong increase. Syndecan-1 and -2 expressions decreased, whereas syndecan-3 was unaffected. Knockdown of syndecan-4 using siRNA resulted in changes in cellular morphology and focal adhesion sites, delayed wound healing and tube formation, and increased secretion of the pro-inflammatory and angiogenic chemokine, CXCL8. These data suggest functions for syndecan-4 in inflammatory reactions, wound healing and angiogenesis in primary human endothelial cells.
authors.affiliation: Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (TTV, TMR, SOK)
authors.forename: Tram Thu
authors.initials: TT
authors.lastname: Vuong
authors.affiliation: Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (TTV, TMR, SOK)
authors.forename: Trine M
authors.initials: TM
authors.lastname: Reine
authors.affiliation: Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (AS)
authors.forename: Amanda
authors.initials: A
authors.lastname: Sudworth
authors.affiliation: Department of Transplant Medicine, Section of Nephrology, Oslo University Hospital, Oslo, Norway (TGJ)|Renal and Metabolic Research Group, Department of Clinical Medicine, UIT The Arctic University of Norway, Tromsø, Norway (TGJ)
authors.forename: Trond G
authors.initials: TG
authors.lastname: Jenssen
authors.affiliation: Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway (TTV, TMR, SOK)
authors.forename: Svein O
authors.initials: SO
authors.lastname: Kolset
mesh_terms: D055785:Gene Knockdown Techniques; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D034741:RNA, Small Interfering / Q000235:genetics; D053669:Syndecan-2 / Q000378:metabolism; D053670:Syndecan-3 / Q000378:metabolism; D053671:Syndecan-4 / Q000235:genetics / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D053670:Syndecan-3; D053671:Syndecan-4; D053669:Syndecan-2
keywords: angiogenesis; inflammation; primary human endothelial cells; shedding; syndecan-4; wound healing