title: Ammonia-oxidizing bacteria and archaea in wastewater treatment plant sludge and nearby coastal sediment in an industrial area in China.
abstract: Under the increasing pressure of human activities, Hangzhou Bay has become one of the most seriously polluted waters along China's coast. Considering the excessive inorganic nitrogen detected in the bay, in this study, the impact of an effluent from a coastal industrial park on ammonia-oxidizing microorganisms (AOMs) of the receiving area was interpreted for the first time by molecular technologies. Revealed by real-time PCR, the ratio of archaeal amoA/bacterial amoA ranged from 5.68 × 10(-6) to 4.79 × 10(-5) in the activated sludge from two wastewater treatment plants (WWTPs) and 0.54-3.44 in the sediments from the effluent receiving coastal area. Analyzed by clone and pyrosequencing libraries, genus Nitrosomonas was the predominant ammonia-oxidizing bacteria (AOB), but no ammonia-oxidizing archaea (AOA) was abundant enough for sequencing in the activated sludge from the WWTPs; genus Nitrosomonas and Nitrosopumilus were the dominant AOB and AOA, respectively, in the coastal sediments. The different abundance of AOA but similar structure of AOB between the WWTPs and nearby coastal area probably indicated an anthropogenic impact on the microbial ecology in Hangzhou Bay.
authors.affiliation: School of Environment, Tsinghua University, Beijing, 100084, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lujun
authors.initials: L
authors.lastname: Chen
authors.forename: Renhua
authors.initials: R
authors.lastname: Sun
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Dai
authors.forename: Jinping
authors.initials: J
authors.lastname: Tian
authors.forename: Donghui
authors.initials: D
authors.lastname: Wen
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D044822:Biodiversity; D002681:China; D019015:Geologic Sediments / Q000382:microbiology*; D007220:Industrial Waste / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000737:chemistry; D018508:Water Purification / Q000295:instrumentation
chemical_list: D000641:Ammonia; D007220:Industrial Waste; D012722:Sewage; D062065:Wastewater