title: MiR-221-induced PUMA silencing mediates immune evasion of bladder cancer cells.
abstract: Immune evasion of cancer cells is mainly due to the impaired transduction of apoptotic signals from immune cells to cancer cells, as well as inhibition of subsequent apoptosis signal cascades within the cancer cells. Over the past few decades, the research has focused more on the impaired transduction of the apoptotic signal from immune cells to cancer cells, rather than inhibition of the intracellular signaling pathways. In this study, miR‑221 inhibitor was transfected into bladder cancer cell lines 5637, J82 and T24 to repress the expression of miR‑221. As a result, the repression of miR‑221 on p53 upregulated modulator of apoptosis (PUMA) was abolished, resulting in increased expression of the pro-apoptotic Bax and reduced expression of the anti-apoptotic Bcl-2, which promotes apoptosis of bladder cancer cells. The expression of MMP-2, MMP-9 and VEGF-C were reduced, resulting in reduced invasiveness and infiltration capability of bladder cancer cells, thereby inhibiting the immune evasion of bladder cancer cells.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Fu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Laboratory Animal Science, Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Xiali
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Health Sciences, Macao Polytechnic Institute, Macao 999078, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of System Bio-medicine of Jiangxi Province, Medical College of Jiujiang University, Jiangxi, Jiujiang 332000, P.R. China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Jiangxi Provincil People's Hospital, Nanchang 330006, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Weipeng
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Urology, Chinese PLA General Hospital, Beijing 100039, P.R. China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Ju
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, P.R. China.
authors.forename: Gongxian
authors.initials: G
authors.lastname: Wang
mesh_terms: D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000502:physiology*; D009361:Neoplasm Invasiveness; D020320:Oligoribonucleotides, Antisense / Q000235:genetics / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D034622:RNA Interference / Q000187:drug effects / Q000502:physiology; D014407:Tumor Cells, Cultured; D019139:Tumor Escape / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D051017:Apoptosis Regulatory Proteins; D035683:MicroRNAs; D020320:Oligoribonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C494826:BAX protein, human; C438378:BBC3 protein, human; C525709:MIR221, human