title: Tadehaginoside modulates lipogenesis and glucose consumption in HepG2 cells.
abstract: Tadehaginoside (TS) is a phenylpropanoid glycoside found in Tadehagi triquetrum, a medicinal plant with multiple biological activities. This study investigated the effect of TS on lipogenesis and glucose consumption in HepG2 cells. Treatment with TS inhibited lipid accumulation in a dose-dependent manner. This inhibition was closely associated with the downregulation of lipogenic genes such as SREBP-1a, SREBP-2 and their downstream targets (FAS, ACC, HMGR) and the upregulation of lipolytic gene PPARα as revealed by real-time quantitative PCR. Further investigation showed that TS significantly stimulated glucose consumption by HepG2 cells and glucose uptake by C2C12 myotubes, which could be partially explained by the upregulation of PPARγ. Collectively, these results clearly indicate that TS is an effective regulator of lipogenesis and glucose consumption, which could be useful in treatment of obesity and diabetes.
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.
authors.forename: Xiaopo
authors.initials: X
authors.lastname: Zhang
authors.affiliation: b Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce , Harbin 150076 , P.R. China.|c Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100193 , P.R. China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.|b Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce , Harbin 150076 , P.R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.|b Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce , Harbin 150076 , P.R. China.
authors.forename: Ni
authors.initials: N
authors.lastname: An
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.|b Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce , Harbin 150076 , P.R. China.
authors.forename: Jiani
authors.initials: J
authors.lastname: Wu
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: c Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , Beijing 100193 , P.R. China.
authors.forename: Chongming
authors.initials: C
authors.lastname: Wu
authors.affiliation: a School of Pharmaceutical Science, Hainan Medical University , Haikou 571101 , P.R. China.
authors.forename: Youbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D003373:Coumaric Acids / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry*; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D050155:Lipogenesis / Q000187:drug effects*; D015394:Molecular Structure; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D003373:Coumaric Acids; D005960:Glucosides; D014157:Transcription Factors; C000595638:tadehaginoside; D005947:Glucose
keywords: glucose consumption; lipogenesis; lipolysis; tadehaginoside