title: [Dynamic changes of a group of cytokines in phosgene-induced lung injury and the function of ulinastatin].
abstract: OBJECTIVE
  To investigate the dynamic changes of a group of cytokines in phosgene-induced lung injury and the function of different dose of ulinastatin through animal experiment.
  
  
  METHODS
  104 male SD rats were randomly assigned into the control group, ulinastatin control group, phosgene treatment groups and different dose of ulinastatin intervention groups, 8 rats each group. Treatment groups were dynamic constant exposure in phosgene, and immediately injected ulinastatin intraperitoneal, and then the experimental animal, the lung tissue biopsy, lung wet/dry ratio, RT-PCR detection, the plasma for detection of Bio-Plex 18 cytokines.
  
  
  RESULTS
  Compared with the control group, plasma concentrations of IL-1α, IL-6, GM-CSF, TNF-α, INF-γ, MIP-3α, VEGF were increased significantly first (2 h), and gradually decreased with the passage of time , the difference was statistically significant (P < 0.05). Plasma concentrations of IL-4, IL-10 were decreased earlier (2h, 6 h) and increased later (24 h) (P < 0.05). The change of plasma concentration of IL-13 was not obvious earlier (2 h) and still rising later (24h), the difference was statistically significant (P < 0.05). After drug intervention, the levels of pro-inflammatory cytokines declined and the levels of anti-inflammatory cytokines raise by different degrees at different times in ulinastatin intervention groups, the difference was statistically significant. The degree of lung injury was improved than the phosgene treatment groups and better in high dose of ulinastatin intervention group. The expression of IL-10, IL-4, IL-13-mRNA of tissue increased in accordance with plasma results.
  
  
  CONCLUSIONS
  A group of cytokines are dynamicly changed in phosgene-induced lung injury by time. High dose of ulinastatin can improved phosgene-induced lung injury, regulate the synthesis and release of inflammatory cytokines and inhibit inflammatory react in a dose-dependent manner.
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Zhiyue
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Daikun
authors.initials: D
authors.lastname: He
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Emergency & Intensive Care Centre for Chemical Accident of Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China. E-mail: j1999sh@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism*; D006023:Glycoproteins / Q000494:pharmacology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016753:Interleukin-10; D018793:Interleukin-13; D053582:Interleukin-1alpha; D015847:Interleukin-4; D015850:Interleukin-6; D008168:Lung; D055370:Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D008297:Male; D010705:Phosgene / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014409:Tumor Necrosis Factor-alpha
chemical_list: D016207:Cytokines; D006023:Glycoproteins; D018793:Interleukin-13; D053582:Interleukin-1alpha; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D010705:Phosgene; D016753:Interleukin-10; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C028665:urinastatin