title: Deficiency of BPOZ2 Decreases Liver Fibrosis After Chronic Carbon Tetrachloride Administration in Mice.
abstract: Bood POZ containing gene type 2 (BPOZ2), a Broad-Complex, Tramtrack, and Bric a brac domain containing protein, is an adaptor protein for the E3 ubiquitin ligase scaffold protein CUL3. It plays an important role in acute carbon tetrachloride (CCl4)-induced liver injury and regeneration in mice. In this study, we investigated the role of BPOZ2 in the process of liver fibrosis induced by chronic CCl4 treatment. The results indicate that BPOZ2 deficiency decreases sustained activation of hepatic stellate cells, attenuates collagen αI(I) and tissue inhibitor of matrix metalloprotease 1 expression, and decreases liver fibrosis after repeated CCl4 administration. These findings suggest BPOZ2 as a new therapeutic target for the prevention and treatment of hepatic fibrosis in chronic liver disease.
authors.affiliation: Department of Clinical Laboratory, Quzhou People's Hospital, Quzhou, Zhejiang, China State Key Laboratory of Medical Genomics, Research Centre for Experimental Medicine of Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Model Organism Division, Department of Medical Genetics, E-Institutes of Shanghai Universities, Shanghai Jiao Tong University School of Medicine, Shanghai, China suyingdang@shsmu.edu.cn.
authors.forename: Suying
authors.initials: S
authors.lastname: Dang
authors.affiliation: State Key Laboratory of Medical Genomics, Research Centre for Experimental Medicine of Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Runzhe
authors.initials: R
authors.lastname: Shu
authors.affiliation: Model Organism Division, Department of Medical Genetics, E-Institutes of Shanghai Universities, Shanghai Jiao Tong University School of Medicine, Shanghai, China Shanghai Research Center for Model Organisms, Shanghai, China.
authors.forename: Yougui
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: Shanghai Research Center for Model Organisms, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: Shanghai Research Center for Model Organisms, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Fei
authors.affiliation: State Key Laboratory of Medical Genomics, Research Centre for Experimental Medicine of Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Institute of Health Sciences, Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China Model Organism Division, Department of Medical Genetics, E-Institutes of Shanghai Universities, Shanghai Jiao Tong University School of Medicine, Shanghai, China Shanghai Research Center for Model Organisms, Shanghai, China zhugangw@shsmu.edu.cn.
authors.forename: Zhugang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002251:Carbon Tetrachloride / Q000633:toxicity*; D003094:Collagen / Q000032:analysis; D005260:Female; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D008103:Liver Cirrhosis / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009687:Nuclear Proteins / Q000172:deficiency* / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C585874:Abtb2 protein, mouse; D009687:Nuclear Proteins; D003094:Collagen; D002251:Carbon Tetrachloride
keywords: BPOZ2; HSC; TIMP-1; carbon tetrachloride; liver fibrosis