title: Investigation of the automated solid-phase synthesis of a 38mer peptide with difficult sequence pattern under different synthesis strategies.
abstract: Difficult peptides are a constant challenge in solid-phase peptide synthesis. In particular, hydroxyl amino acids such as serine can cause severe breakdowns in coupling yields even several amino acids after the insertion of the critical amino acid. This paper investigates several methods of improving synthesis yields of difficult peptides including the use of different resins, activators and the incorporation of a structure-breaking pseudoproline dipeptide building block both alone and in combination with each other.
authors.affiliation: Peptide Facility, Kinexus Bioinformatics Corporation, 8755 Ash Street, Suite 1, Vancouver, BC, V6P 6T3, Canada, dr.d.winkler@web.de.
authors.forename: Dirk F H
authors.initials: DF
authors.lastname: Winkler
authors.forename: Kerry
authors.initials: K
authors.lastname: Tian
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D001331:Automation / Q000379:methods*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
chemical_list: D000596:Amino Acids; D010455:Peptides