title: Cytoskeleton remodelling of confluent epithelial cells cultured on porous substrates.
abstract: The impact of substrate topography on the morphological and mechanical properties of confluent MDCK-II cells cultured on porous substrates was scrutinized by means of various imaging techniques as well as atomic force microscopy comprising force volume and microrheology measurements. Regardless of the pore size, ranging from 450 to 5500 nm in diameter, cells were able to span the pores. They did not crawl into the holes or grow around the pores. Generally, we found that cells cultured on non-porous surfaces are stiffer, i.e. cortical tension rises from 0.1 to 0.3 mN m(-1), and less fluid than cells grown over pores. The mechanical data are corroborated by electron microscopy imaging showing more cytoskeletal filaments on flat samples in comparison to porous ones. By contrast, cellular compliance increases with pore size and cells display a more fluid-like behaviour on larger pores. Interestingly, cells on pores larger than 3500 nm produce thick actin bundles that bridge the pores and thereby strengthen the contact zone of the cells.
authors.affiliation: Institute of Physical Chemistry, University of Goettingen, Tammannstrasse 6, Göttingen 37077, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Rother
authors.affiliation: I. Institute of Physics, University of Goettingen, Friedrich-Hund Platz 1, Göttingen 37077, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Büchsenschütz-Göbeler
authors.affiliation: Institute of Physical Chemistry, University of Goettingen, Tammannstrasse 6, Göttingen 37077, Germany.
authors.forename: Helen
authors.initials: H
authors.lastname: Nöding
authors.affiliation: Research Center CAESAR, Ludwig-Erhard-Allee 2, Bonn 53175, Germany.
authors.forename: Siegfried
authors.initials: S
authors.lastname: Steltenkamp
authors.affiliation: I. Institute of Physics, University of Goettingen, Friedrich-Hund Platz 1, Göttingen 37077, Germany.
authors.forename: Konrad
authors.initials: K
authors.lastname: Samwer
authors.affiliation: Institute of Physical Chemistry, University of Goettingen, Tammannstrasse 6, Göttingen 37077, Germany ajansho@gwdg.de.
authors.forename: Andreas
authors.initials: A
authors.lastname: Janshoff
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D061985:Madin Darby Canine Kidney Cells; D016062:Porosity
keywords: MDCK-II cells; atomic force microscopy; cytoskeleton; microrheology; porous substrates; tension