title: Application of Savitzky-Golay differentiation filters and Fourier functions to simultaneous determination of cefepime and the co-administered drug, levofloxacin, in spiked human plasma.
abstract: The present work is concerned with simultaneous determination of cefepime (CEF) and the co-administered drug, levofloxacin (LEV), in spiked human plasma by applying a new approach, Savitzky-Golay differentiation filters, and combined trigonometric Fourier functions to their ratio spectra. The different parameters associated with the calculation of Savitzky-Golay and Fourier coefficients were optimized. The proposed methods were validated and applied for determination of the two drugs in laboratory prepared mixtures and spiked human plasma. The results were statistically compared with reported HPLC methods and were found accurate and precise.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Omar
authors.initials: O
authors.lastname: Abdel-Aziz
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
authors.forename: Maha F
authors.initials: MF
authors.lastname: Abdel-Ghany
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. Electronic address: drreham2003@gmail.com.
authors.forename: Reham
authors.initials: R
authors.lastname: Nagi
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Misr University for Science and Technology, Giza, Egypt.
authors.forename: Laila
authors.initials: L
authors.lastname: Abdel-Fattah
mesh_terms: D000077723:Cefepime; D002511:Cephalosporins / Q000097:blood*; D005583:Fourier Analysis; D006801:Humans; D064704:Levofloxacin / Q000097:blood*; D008958:Models, Molecular; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
chemical_list: D002511:Cephalosporins; D012996:Solutions; D064704:Levofloxacin; D000077723:Cefepime
keywords: Cefepime; Fourier; Levofloxacin; Plasma; Savitzky–Golay; Spectrophotometry