title: Prescription opioids. III. Disposition of oxycodone in oral fluid and blood following controlled single-dose administration.
abstract: Oxycodone (OC) is recommended to be included as an analyte tested in the proposed Substance Abuse and Mental Health Services Administration (SAMHSA's) Mandatory Guidelines for Federal Workplace Drug Testing Programs using Oral Fluid (OF) Specimens. This study demonstrates the time course of OC and metabolites, noroxycodone (NOC), oxymorphone (OM) and noroxymorphone (NOM), in near-simultaneous paired OF and whole blood (BL) specimens by liquid chromatography-tandem mass spectrometry (LC-MS-MS) (limit of detection = 1 ng/mL OF, 5 ng/mL BL). A single dose of OC 20 mg controlled-release was administered to 12 healthy subjects followed by specimen collections for 52 h. Analyte prevalence was as follows: OF, OC > NOC > OM; and BL, OC > NOC > NOM. OC and NOC were frequently detected within 15-30 min in OF and 30 min to 2 h in BL. NOM and OM appeared between 1.5-5 h post-dose. The mean OF-to-BL (OF:BL) ratios and correlations were 5.4 for OC (r = 0.719) and 1.0 for NOC (r = 0.651). The period of detection for OF exceeded BL by ∼2-fold at similar cutoff concentrations. At a 1 ng/mL cutoff for OF, the mean detection time was 34 h for OC and NOC. These data provide new information that should facilitate interpretation of OC test results.
authors.affiliation: Johns Hopkins School of Medicine, Baltimore, MD, USA.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Cone
authors.affiliation: Aegis Sciences Corporation, 515 Great Circle Road, Nashville, TN 37228, USA College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN, USA anne.depriest@aegislabs.com.
authors.forename: Anne Z
authors.initials: AZ
authors.lastname: DePriest
authors.affiliation: Aegis Sciences Corporation, 515 Great Circle Road, Nashville, TN 37228, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Heltsley
authors.affiliation: Aegis Sciences Corporation, 515 Great Circle Road, Nashville, TN 37228, USA Department of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, TN, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Black
authors.affiliation: RTI International, Research Triangle Park, NC, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Mitchell
authors.affiliation: Division of Workplace Programs (DWP), Substance Abuse and Mental Health Services Administration (SAMHSA), Rockville, MD, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: LoDico
authors.affiliation: Division of Workplace Programs (DWP), Substance Abuse and Mental Health Services Administration (SAMHSA), Rockville, MD, USA.
authors.forename: Ron
authors.initials: R
authors.lastname: Flegel
mesh_terms: D000284:Administration, Oral; D000701:Analgesics, Opioid / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D001711:Biotransformation; D002853:Chromatography, Liquid; D003692:Delayed-Action Preparations; D004334:Drug Administration Schedule; D006801:Humans; D016272:Occupational Health; D010098:Oxycodone / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D055553:Prescription Drugs / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods; D053719:Tandem Mass Spectrometry; D014018:Tissue Distribution; D017132:Workplace
chemical_list: D000701:Analgesics, Opioid; D003692:Delayed-Action Preparations; D055553:Prescription Drugs; D010098:Oxycodone