title: Hair dyes resorcinol and lawsone reduce production of melanin in melanoma cells by tyrosinase activity inhibition and decreasing tyrosinase and microphthalmia-associated transcription factor (MITF) expression.
abstract: Hair coloring products are one of the most important cosmetics for modern people; there are three major types of hair dyes, including the temporary, semi-permanent and permanent hair dyes. The selected hair dyes (such as ammonium persulfate, sodium persulfate, resorcinol and lawsone) are the important components for hair coloring products. Therefore, we analyzed the effects of these compounds on melanogenesis in B16-F10 melanoma cells. The results proved that hair dyes resorcinol and lawsone can reduce the production of melanin. The results also confirmed that resorcinol and lawsone inhibit mushroom and cellular tyrosinase activities in vitro. Resorcinol and lawsone can also downregulate the protein levels of tyrosinase and microphthalmia-associated transcription factor (MITF) in B16-F10 cells. Thus, we suggest that frequent use of hair dyes may have the risk of reducing natural melanin production in hair follicles. Moreover, resorcinol and lawsone may also be used as hypopigmenting agents to food, agricultural and cosmetic industry in the future.
authors.affiliation: Department of Cosmetic Science and Management, Mackay Medicine, Nursing and Management College, 92 Shengjing Road, Beitou, Taipei 11260, Taiwan. s107@eip.mkc.edu.tw.
authors.forename: Shu-Mei
authors.initials: SM
authors.lastname: Lee
authors.affiliation: Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, Taiwan. yishyan@gm.pu.edu.tw.
authors.forename: Yi-Shyan
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, Taiwan. chchlin@pu.edu.tw.
authors.forename: Chih-Chien
authors.initials: CC
authors.lastname: Lin
authors.affiliation: Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, Taiwan. happyalan12@gmail.com.
authors.forename: Kuan-Hung
authors.initials: KH
authors.lastname: Chen
mesh_terms: D000645:Ammonium Sulfate / Q000633:toxicity; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents / Q000633:toxicity*; D015536:Down-Regulation / Q000187:drug effects*; D008543:Melanins / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009285:Naphthoquinones / Q000633:toxicity*; D012118:Resorcinols / Q000633:toxicity*; D017670:Sodium Compounds / Q000633:toxicity; D013431:Sulfates / Q000633:toxicity
chemical_list: D004396:Coloring Agents; D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; D009285:Naphthoquinones; D012118:Resorcinols; D017670:Sodium Compounds; D013431:Sulfates; C031276:ammonium peroxydisulfate; D014442:Monophenol Monooxygenase; C024625:sodium persulfate; D000645:Ammonium Sulfate; C005090:lawsone; C031389:resorcinol