title: Involvement of RalB in the effect of geranylgeranyltransferase I on glioma cell migration and invasion.
abstract: OBJECTIVE
  The present study aimed at investigating the role and potential mechanism of geranylgeranyltransferase I (GGTase-I) on glioma cell migration and invasion.
  
  
  METHODS
  Wound-healing assay and transwell assay were performed to study whether GGTase-I and Ras-like GTPase B (RalB) have effect on glioma cell migration and invasion.
  
  
  RESULTS
  We found that knockdown of GGTase-I or RalB both significantly decreased the migratory and invasive abilities of glioma cells. GGTase-I down-regulation suppressed RalB membrane association. Moreover, down-regulation of RalB partially abolished the effect of GGTβ over-expression-induced glioma cell migration and invasion increase.
  
  
  CONCLUSIONS
  These findings suggest that RalB might be one of the targets for facilitating the invasive phenotype of malignant gliomas induced by GGTase-I.
authors.affiliation: Institute of Nervous System Diseases, Xuzhou Medical College, 84 West Huai-hai Road, Xuzhou, 221002, Jiangsu, People's Republic of China.
authors.forename: X
authors.initials: X
authors.lastname: Song
authors.forename: L
authors.initials: L
authors.lastname: Hua
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
authors.forename: Z
authors.initials: Z
authors.lastname: Fang
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Gao
authors.forename: Q
authors.initials: Q
authors.lastname: Shi
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: R
authors.initials: R
authors.lastname: Yu
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D005910:Glioma / Q000473:pathology*; D006801:Humans; D009361:Neoplasm Invasiveness / Q000473:pathology*; D034741:RNA, Small Interfering; D014162:Transfection; D020725:ral GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C483163:Ralb protein, human; D019883:Alkyl and Aryl Transferases; C083291:geranylgeranyltransferase type-I; D020725:ral GTP-Binding Proteins