title: Outer membrane proteome analysis of Indian strain of Pasteurella multocida serotype B:2 by MALDI-TOF/MS analysis.
abstract: Identification of outer membrane proteins (OMPs) is important to understand the bacteria structure and function, host-pathogen interaction, development of novel vaccine candidates, and diagnostic antigens. But till now the key antigens of P. multocida B:2 isolate causing haemorrhagic septicaemia (HS) in animals are not clearly defined. In this study, P52 strain of P. multocida serotype B:2 was grown in vitro under iron-rich and iron-limited condition. The OMPs were extracted by sarkosyl method followed by SDS-PAGE and the proteins were identified by MALDI-TOF/MS analysis. In total, 22 proteins were identified, of which 7 were observed exclusively under iron-limited condition. Most of the high molecular weight proteins (TbpA, HgbA, HgbB, HasR, IroA, and HemR) identified in this study were involved in iron acquisition. Some hypothetical proteins (HP-KCU-10206, HP and AAUPMB 08244, HP AAUPMB 21592, HP AAUPMB 19766, AAUPMB 11295) were observed for the first time in this study which could be unique to serotype B:2. Further functional in vivo study of the proteins identified are required to explore the utility of these proteins in developing diagnostics and vaccine against HS.
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: A
authors.initials: A
authors.lastname: Prasannavadhana
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India ; Institute of Bacterial Infections and Zoonoses, Friedrich Loeffler Institut, Namburger Straße 96 a, 07743 Jena, Germany.
authors.forename: Prasad
authors.initials: P
authors.lastname: Thomas
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India ; Regional Medical Research Centre, Bhubaneswar, Odisha 751023, India.
authors.forename: Laxmi Narayan
authors.initials: LN
authors.lastname: Sarangi
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Santosh Kumar
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Adyasha
authors.initials: A
authors.lastname: Priyadarshini
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Viswas Konasagara
authors.initials: VK
authors.lastname: Nagaleekar
authors.affiliation: Division of Veterinary Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India.
authors.forename: Vijendra Pal
authors.initials: VP
authors.lastname: Singh
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000378:metabolism*; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology; D007194:India; D007501:Iron / Q000494:pharmacology; D016979:Pasteurella multocida / Q000145:classification* / Q000187:drug effects; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D065288:Serogroup; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D016056:Immunodominant Epitopes; D020543:Proteome; D007501:Iron