title: Immunolocalization of CD34 Positive Progenitor Cells in Diabetic and Non Diabetic Periodontitis Patients - A Comparative Study.
abstract: BACKGROUND
  Little research has been documented to determine the CD34 positive cells in healthy periodontium, chronic periodontitis and in chronic periodontitis with diabetes mellitus individuals.
  
  
  OBJECTIVE
  The aim of the present study was to evaluate and compare the CD34 positive progenitor cells of the gingiva in patients with healthy periodontium, chronic periodontitis and chronic periodontitis with Diabetes Mellitus.
  
  
  METHODS
  A total number of 75 patients were divided into 3 groups which included Group I (healthy periodontium), Group II (chronic periodontitis) and Group III (chronic periodontitis with diabetes mellitus). Periodontal examination included Plaque index, Gingival index, Gingival bleeding index, Probing pocket depth and Clinical attachment levels. Gingival biopsies were collected from each participant and they were fixed in formalin embedded in paraffin which was later subjected to immunohistochemical procedure with anti-CD34 (a stemness marker). T-Test and Regression analysis (R-square) was used to analyse the data.
  
  
  RESULTS
  The mean number of CD34 positive cells were higher in group III (5.71±1.97) compared to Group chronic periodontitis group I (4.98± 2.08) and II (4.48± 1.24).
  
  
  CONCLUSIONS
  Although CD34 is a non specific stemness marker, results suggest that there is a significant difference in the number of CD34 positive progenitor cells between Group II and Group III but no significant difference was observed between Group I, II and Group I, III.
authors.affiliation: Professor, Department of Periodontics, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Gautami S
authors.initials: GS
authors.lastname: Penmetsa
authors.affiliation: Reader, Department of Conservative Dentistry & Endodontics, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Narendra
authors.initials: N
authors.lastname: Mandalapu
authors.affiliation: Postgraduate Student, Department of Periodontics, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Sitaramaraju
authors.initials: S
authors.lastname: Dv
authors.affiliation: Associate Professor, Department of Oral Pathology, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Ravikanth
authors.initials: R
authors.lastname: Mannem
authors.affiliation: Assistant Professor, Department of Dental Materials, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Rama Krishna
authors.initials: RK
authors.lastname: Alla
authors.affiliation: Senior Lecturer, Department of Public Health Dentistry, Vishnu Dental College , Bhimavaram, Andhra Pradesh, India .
authors.forename: Praveen
authors.initials: P
authors.lastname: Gadde
keywords: CD34; Chronic Periodontitis; Diabetes Mellitus; Mesenchymal Progenitor cells; Paravascular and Stromal Progenitor cells