title: Fabrication and characterization of ovalbumin films for wound dressing applications.
abstract: A great number of people suffer from burning injuries all around the world each year. Applying an appropriate wound dressing can promote new tissue formation, prevent losing water and inhibit invasion of infectious organisms. In this study, egg white with a long standing history, as a homemade remedy, was fabricated as a wound dressing for burn injuries. For this reason, ovalbumin films were cross-linked by 1-ethyl-3-3-dimethyl aminopropyl carbodiimide hydrochloride (EDC) with different concentrations (1, 5 and 10mM) using three concentrations of ethanol. Physical-chemical characterizations including Fourier transform infrared spectroscopy (FTIR), gas transmission rate (GTR), tensile mechanical tests, water uptake and degradation rate were performed on the samples. The sample with 5mM crosslinking agent at 70% ethanol was considered as the optimized one with 417kPa of ultimate tensile strength, 64% elongation at break and 230% water uptake. In addition, biological evaluations conducted by MTT and live/dead assay indicated no sign of cyto-toxicity for all the samples. Moreover, scanning electron microscopy (SEM) showed that the fibroblast cells were well spread on the sample with the formation of filopodia. In conclusion, modified ovalbumin can be applied as the base material for fabrication of wound dressing and skin care products.
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mozhgan
authors.initials: M
authors.lastname: Shojaee
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Navaee
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran; Departments of Biomedicine and Surgery, University Hospital Basel, University of Basel, 4031 Basel, Switzerland.
authors.forename: Sasan
authors.initials: S
authors.lastname: Jalili-Firoozinezhad
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Faturechi
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Majidi
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran. Electronic address: sh_bonakdar@pasteur.ac.ir.
authors.forename: Shahin
authors.initials: S
authors.lastname: Bonakdar
mesh_terms: D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D002460:Cell Line / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010047:Ovalbumin / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D010047:Ovalbumin; D005022:Ethyldimethylaminopropyl Carbodiimide
keywords: Biocompatibility; Burn; Ovalbumin; Wound dressing