title: The level of Alzheimer-associated neuronal thread protein in urine may be an important biomarker of mild cognitive impairment.
abstract: Increased levels of Alzheimer-associated neuronal thread protein (AD7c-NTP) are often detected in urine in the early course of Alzheimer's disease (AD), which makes it a promising biomarker for AD. However, whether the concentration of urinary AD7c-NTP is increased in patients with mild cognitive impairment (MCI) remains unclear. The aim of this study was to explore the value of urinary AD7c-NTP to assist in the diagnosis of cognitive impairment by comparing differences in urinary AD7c-NTP among normal controls, MCI patients and AD patients. One hundred and seventy patients from the Xuan wu Hospital, Capital Medical University were divided into three groups according to their clinical diagnosis: an AD group (n=45), an MCI group (n=60) and a normal group (n=65). The Mini Mental State Examination and the Montreal Cognitive Assessment scale were used to screen for the diagnosis of AD and MCI, and patients met the diagnostic criteria of the National Institute of Neurological Disorders and Stroke and the Alzheimer's Disease and Related Disorders Association. The level of urinary AD7c-NTP was determined using the enzyme-linked immunosorbent assay method. The urinary levels of AD7c-NTP in the AD group (median 2.14 [range 0.49-6.39] ng/ml) and the MCI group (median 1.57 [range 0.4-4.15] ng/ml) were significantly higher than those of the normal group (median 0.53 [range 0.04-2.07] ng/ml). To our knowledge our study is the first to show that the level of urinary AD7c-NTP in MCI patients is higher than in healthy people, which suggests that the level of urinary AD7c-NTP may be an important biomarker for early diagnosis of MCI.
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China; Department of Geriatrics, Xuan Wu Hospital, Capital Medical University, Beijing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Ma
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China; Division of Respiratory Disease, Mentougou District Hospital, Beijing, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China. Electronic address: rong_wang72@aliyun.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xuan Wu Hospital, Capital Medical University, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Han
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Jingshuang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Dong
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Yanchuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Central Laboratory, Xuan Wu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Key Laboratory for Neurodegenerative Disease of Ministry of Education, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, 45 Changchun Street, Xicheng District, Beijing 100053, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000652:urine*; D015415:Biomarkers / Q000652:urine*; D060825:Cognitive Dysfunction / Q000652:urine*; D003704:Dementia / Q000175:diagnosis / Q000523:psychology; D042241:Early Diagnosis; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000652:urine*; D009483:Neuropsychological Tests
chemical_list: C497612:AD7c-NTP protein, human; D015415:Biomarkers; D009419:Nerve Tissue Proteins
keywords: Alzheimer associated neuronal thread protein; Alzheimer’s disease; ELISA; Mild cognitive impairment; Urine