title: Determination of isobavachalcone in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
abstract: A simple and selective specific high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of isobavachalcone (IBC) in rat plasma was developed. Neobavaisoflavone was used as an internal standard (IS). After protein precipitation with acetonitrile (2:1, v/v), the analyte and IS were separated on a 2.6 μm Kinetex C18 column (100 mm×2.1 mm i.d., Phenomenex) by isocratic elution with acetonitrile:water (60:40, v/v) as the mobile phase at a flow rate of 0.2 mL/min. An electrospray ionization (ESI) source was applied and operated in the negative ion mode; multiple reactions monitoring (MRM) mode was used for quantification, and the target fragment ions m/z 323.0→118.9 for IBC and m/z 321.1→265.0 for the IS were chosen. Good linearity was observed in the concentration range of 3.79-484.5 ng/mL for IBC in rat plasma. The recovery of IBC in plasma was in the range of 81.2-89.8%. Intra-day and inter-day precision were both lower than 10%. This method was suitable for pharmacokinetic studies after oral administration of 80 mg/kg IBC in rats. We also obtained pharmacokinetic parameters and concentration-time profiles for IBC after oral administration of IBC in rats.
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, Anhui, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, Anhui, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Nie
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, Anhui, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Li
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, Anhui, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Huo
authors.affiliation: Department of Biochemistry, Bengbu Medical College, 2600 Donghai Road, Bengbu, Anhui, China. Electronic address: zhangyuxin_bbmc@foxmail.com.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Faculty of Pharmacy, Bengbu Medical College, 2600 Donghai Road, Bengbu 233030, Anhui, China. Electronic address: wuchengzhu0611@foxmail.com.
authors.forename: Cheng-Zhu
authors.initials: CZ
authors.lastname: Wu
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D047188:Chalcones / Q000097:blood* / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D005260:Female; D007477:Ions / Q000737:chemistry; D007529:Isoflavones / Q000097:blood / Q000493:pharmacokinetics; D008297:Male; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012015:Reference Standards; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000097:Acetonitriles; D047188:Chalcones; D007477:Ions; D007529:Isoflavones; C549830:neobavaisoflavone; C468754:isobavachalcone; C032159:acetonitrile
keywords: Electrospray ionization; Isobavachalcone; LC–MS/MS; Pharmacokinetics; Rat plasma