title: Liquid Chromatography-Tandem Mass Spectrometry Screening Method for the Detection of Radical-Scavenging Natural Antioxidants from the Whole Scutellariae (Radix, Stem and Leaf).
abstract: A novel free radical reaction combined with high-performance LC-photodiode array-ESI-MS/MS screening method was developed for the detection and identification of natural antioxidants from whole Scutellariae. Six compounds and whole Scutellariae extracts were found to possess a potential antioxidant capacity, and their free radical-scavenging activities were investigated in detail. The six compounds were identified as baicalin, baicalein, scutellarin, scutellarein, wogonoside and chrystin-7-glucoronide. The present study reveals that the radical-scavenging capacities of the whole Scutellariae extracts are as follows: hydroxyl radical > superoxide radical > peroxy radical. Wogonoside showed the strongest capability for scavenging hydroxyl radical. Baicalein not only showed the strongest capability for scavenging superoxide radical but also showed capability for lipid radical; Scutellarein (Peak 5) exhibited the highest reactivity in the lipid peroxidation processes. Based on these studies, the current paper accomplishes the evaluation of activities of some important anti-radical substances extracted from the radix, stem and leaf of the whole Scutellariae.
authors.affiliation: School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China.
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Shi
authors.affiliation: School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China.
authors.forename: Rui-Xing
authors.initials: RX
authors.lastname: Yao
authors.affiliation: School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China gywangcn@126.com.
authors.forename: Guo-Ying
authors.initials: GY
authors.lastname: Wang
authors.affiliation: School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China.
authors.forename: Zhen-Ju
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China.
authors.forename: Fu-Wen
authors.initials: FW
authors.lastname: Chen
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D044950:Flavanones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005609:Free Radicals / Q000737:chemistry; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D031364:Scutellaria baicalensis / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D005609:Free Radicals; D005960:Glucosides; D010936:Plant Extracts; C038044:baicalin; C006680:baicalein; C473995:wogonoside