title: Imaging of nucleolar RNA in living cells using a highly photostable deep-red fluorescent probe.
abstract: A new crescent-shape fluorescent probe (named here as CP) that selectively stains RNA in nucleoli of living cells is prepared. CP shows a deep-red emission (658 nm) and a large Stokes shift because of the introduction of rigid-conjugated coumarin moiety into the molecular structure. Cell imaging experiments indicate that CP can rapidly stain nucleoli in living cells by binding with nucleolar RNA, showing performance superior to commercially available nucleoli dye SYTO RNASelect in terms of high photostability and selectivity. More significantly, these excellent properties together with low cytotoxicity enable CP to monitor nucleolar RNA changes during mitosis, and after treating with anti-cancer drugs cisplatin, actinomycin D and α-amanitin. Thus, CP could be a potential tool for real-time, long-term visualization of the dynamic changes for nucleolar RNA and evaluation of the therapeutic effect for anti-cancer drugs that targeted RNA polymerase I (Pol I).
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Bingjiang
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: wangpf@mail.ipc.ac.cn.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D058948:Cell Tracking; D002945:Cisplatin / Q000494:pharmacology; D005456:Fluorescent Dyes; D006801:Humans; D057054:Molecular Imaging; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification*; D012318:RNA Polymerase I / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D012313:RNA; D012318:RNA Polymerase I; D002945:Cisplatin
keywords: Fluorescent imaging; Nucleoli; RNA; Real-time