title: Applying dried blood spot sampling with LCMS quantification in the clinical development phase of tasquinimod.
abstract: BACKGROUND
  Tasquinimod is an orally active anticancer drug in late clinical development. Here we describe the development and validation of a bioanalytical method based upon dried blood spot analysis in combination with LCMS/MS and stable isotope dilution.
  
  
  CONCLUSIONS
  The present method was validated for accuracy, precision, linearity, selectivity, carry-over and ruggedness. Data elucidating stability of tasquinimod in dried blood spots and in blood at ambient temperature was investigated and found adequate. Furthermore, in a clinical study, incurred samples reanalysis was performed, and the correlation of blood concentration versus plasma concentrations of tasquinimod was investigated.
  
  
  CONCLUSIONS
  The method described here is suitable for bioanalysis of tasquinimod in whole blood from humans in clinical studies.
authors.affiliation: Active Biotech AB, Lund, Sweden.
authors.forename: Leif D
authors.initials: LD
authors.lastname: Svensson
authors.forename: Carl-Johan
authors.initials: CJ
authors.lastname: Sennbro
authors.forename: Camilla
authors.initials: C
authors.lastname: Svanström
authors.forename: Gunnar P
authors.initials: GP
authors.lastname: Hansson
mesh_terms: D000970:Antineoplastic Agents / Q000097:blood* / Q000592:standards; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D059788:Dried Blood Spot Testing / Q000592:standards; D006801:Humans; D011786:Quality Control; D011804:Quinolines / Q000097:blood* / Q000592:standards; D015363:Quinolones; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D000970:Antineoplastic Agents; D011804:Quinolines; D015363:Quinolones; C516109:tasquinimod