title: Impact of temporary hyperthermia on corneal endothelial cell survival during organ culture preservation.
abstract: To evaluate temporary exposure to hyperthermia for its impact on endothelial cell density of porcine corneas in organ culture medium containing dextran with regards to possible negative influences of high temperatures during the storage and transport of corneal grafts. Four groups of central discs (diameter 8 mm) from the corneas of both eyes in 40 pigs were first organ-cultured (MEM with 6% dextran 500) for 24 h at 32°C. Ten corneas were then exposed to 40°C in group 1, to 42°C in group 2, to 44°C in group 3, and to 50°C in group 4 for 12 h each. The paired corneal discs for all groups were not treated, stored at 32°C and served as controls. After further organ culture of all corneas for 48 h at 32°C to allow regenerative processes, corneal endothelium was stained with Alizarin Red S and examined by light microscopy. The endothelial cell densities were determined on three central images using a system for the automatic estimation of morphometric parameters of corneal endothelium. Exposure for 12 h to 40°C as well as to 42°C induced no endothelial cell loss. Statistical analysis showed no significant difference of the endothelial cell density between corneas exposed to 40°C and 42°C and the control corneas (40°C treatment: 4736 ± 426 cells/mm(2) and control: 4762 ± 344 cells/mm(2), p = 0.74; 42°C treatment: 4240 ± 363 cells/mm(2) and control: 4176 ± 448 cells/mm(2), p = 0.40). Exposure to 44°C and 50°C lead to total necrosis of the endothelial cell layer. Exposure of organ cultured porcine corneas in dextran containing medium up to 42°C for 12 h does not compromise the endothelial cell density in a clinically relevant manner. Temperatures above 42°C, as it might be the case during transports from the cornea bank to the ophthalmic surgeon, must be strictly avoided as they damage the endothelial cell layer.
authors.affiliation: Institute of Transfusion Medicine, University Tissue Bank, Cornea Bank Berlin, Charité - Universitätsmedizin Berlin, Campus Mitte, Charitéplatz 1, 10117, Berlin, Germany, jan.schroeter@charite.de.
authors.forename: Jan
authors.initials: J
authors.lastname: Schroeter
authors.forename: Alfredo
authors.initials: A
authors.lastname: Ruggeri
authors.forename: Hagen
authors.initials: H
authors.lastname: Thieme
authors.forename: Christian
authors.initials: C
authors.lastname: Meltendorf
mesh_terms: D000818:Animals; D002452:Cell Count; D002470:Cell Survival / Q000502:physiology; D003470:Culture Media; D004728:Endothelium, Corneal / Q000473:pathology; D005125:Eye Banks / Q000379:methods; D006979:Hyperthermia, Induced / Q000009:adverse effects*; D009336:Necrosis; D009924:Organ Culture Techniques; D009926:Organ Preservation / Q000379:methods*; D013552:Swine
chemical_list: D003470:Culture Media