title: Simultaneous orthogonal dual-click approach to tough, in-situ-forming hydrogels for cell encapsulation.
abstract: The use of tough hydrogels as biomaterials is limited as a consequence of time-consuming fabrication techniques, toxic starting materials, and large strain hysteresis under deformation. Herein, we report the simultaneous application of nucleophilic thiol-yne and inverse electron-demand Diels-Alder additions to independently create two interpenetrating networks in a simple one-step procedure. The resultant hydrogels display compressive stresses of 14-15 MPa at 98% compression without fracture or hysteresis upon repeated load. The hydrogel networks can be spatially and temporally postfunctionalized via radical thiylation and/or inverse electron-demand Diels-Alder addition to residual functional groups within the network. Furthermore, gelation occurs rapidly under physiological conditions, enabling encapsulation of human cells.
authors.affiliation: Department of Chemistry, The University of Warwick , Gibbet Hill Road, Coventry CV4 7AL, United Kingdom.
authors.forename: Vinh X
authors.initials: VX
authors.lastname: Truong
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Ablett
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Richardson
authors.forename: Judith A
authors.initials: JA
authors.lastname: Hoyland
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Dove
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018914:Cells, Immobilized / Q000166:cytology; D057930:Click Chemistry; D061565:Cycloaddition Reaction; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008958:Models, Molecular; D011092:Polyethylene Glycols / Q000737:chemistry*; D013314:Stress, Mechanical; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D023822:Tissue Engineering
chemical_list: D001672:Biocompatible Materials; D013438:Sulfhydryl Compounds; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols