title: Influence of modified polyester on the material properties of collagen-based biocomposites and in vitro evaluation of cytocompatibility.
abstract: The cytocompatibility of composite materials collagen (Col)/poly(hydroxyalkanoate) (PHA) and collagen/maleic anhydride-grafted PHA (PHA-g-MA) was investigated in this study. Col was homogeneously dispersed in the PHA-g-MA matrix as a result of condensation reactions. Mechanical characterisation indicated that the improved adhesion between Col and PHA-g-MA enhanced the tensile strength of the composite compared with that of PHA/Col. PHA-g-MA/Col composites were also more water-resistant than PHA/Col composites. Collagen and cell proliferation analysis indicated that PHA and PHA-g-MA and their composites were biocompatible with respect to FB proliferation. Cell-cycle and apoptosis assays by FBs on the PHA series composite samples were not affected by DNA content related to damage, i.e. rapid apoptosis/necrosis was not observed, demonstrating the potential of PHA/Col or PHA-g-MA/Col membranes for biomedical material applications.
authors.affiliation: Department of Chemical and Biochemical Engineering, Kao Yuan University, Kaohsiung County 82101, Taiwan, ROC. Electronic address: t50008@cc.kyu.edu.tw.
authors.forename: Chin-San
authors.initials: CS
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D001672:Biocompatible Materials; D002453:Cell Cycle; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D004249:DNA Damage; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008297:Male; D008299:Maleic Anhydrides / Q000737:chemistry; D008422:Materials Testing / Q000379:methods*; D011091:Polyesters / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry; D013718:Tensile Strength; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D008299:Maleic Anhydrides; D011091:Polyesters; D054813:Polyhydroxyalkanoates; D014867:Water; D003094:Collagen
keywords: Collagen; Cytocompatibility; Polyester; Polymer-matrix composites