title: Glial β-oxidation regulates Drosophila energy metabolism.
abstract: The brain's impotence to utilize long-chain fatty acids as fuel, one of the dogmas in neuroscience, is surprising, since the nervous system is the tissue most energy consuming and most vulnerable to a lack of energy. Challenging this view, we here show in vivo that loss of the Drosophila carnitine palmitoyltransferase 2 (CPT2), an enzyme required for mitochondrial β-oxidation of long-chain fatty acids as substrates for energy production, results in the accumulation of triacylglyceride-filled lipid droplets in adult Drosophila brain but not in obesity. CPT2 rescue in glial cells alone is sufficient to restore triacylglyceride homeostasis, and we suggest that this is mediated by the release of ketone bodies from the rescued glial cells. These results demonstrate that the adult brain is able to catabolize fatty acids for cellular energy production.
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Joachim G
authors.initials: JG
authors.lastname: Schulz
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Antonio
authors.initials: A
authors.lastname: Laranjeira
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Leen
authors.initials: L
authors.lastname: Van Huffel
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Annette
authors.initials: A
authors.lastname: Gärtner
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Sven
authors.initials: S
authors.lastname: Vilain
authors.affiliation: VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Jarl
authors.initials: J
authors.lastname: Bastianen
authors.affiliation: Laboratory of Lipid Biochemistry, KU Leuven, Herestraat 49, 3000 Leuven, Belgium.
authors.forename: Paul P
authors.initials: PP
authors.lastname: Van Veldhoven
authors.affiliation: 1] VIB Center for the Biology of Disease, Leuven and Center for Human Genetics, KU Leuven, Leuven, Belgium [2] Centro Biología Molecular "Severo Ochoa" CSIC-UAM, Madrid, Spain.
authors.forename: Carlos G
authors.initials: CG
authors.lastname: Dotti
mesh_terms: D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure; D002334:Carnitine O-Palmitoyltransferase / Q000737:chemistry / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism; D006706:Homeostasis; D006801:Humans; D007814:Larva / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism; D050356:Lipid Metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009457:Neuroglia / Q000378:metabolism*; D009765:Obesity / Q000473:pathology; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D016019:Survival Analysis; D014280:Triglycerides / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D010743:Phospholipids; D012333:RNA, Messenger; D014280:Triglycerides; D002334:Carnitine O-Palmitoyltransferase