title: Lentivirus-mediated silencing of spindle and kinetochore-associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells.
abstract: Spindle and kinetochore‑associated protein 1 (SKA1) is an important component of the human kinetochore, which plays a key role in mitosis. The resent study was designed to investigate the role of SKA1 in human glioblastoma. The results of the present study demonstrated that SKA1 was expressed in human glioblastoma cells. In addition, the knockdown of SKA1 expression in the A172 and U251 human glioblastoma cell lines was accomplished using a lentivirus infection method. An MTT assay demonstrated that downregulation of SKA1 may inhibit cell proliferation, without affecting the cell cycle. Furthermore, knockdown of SKA1 expression resulted in reduced cell invasion. The results of the present study indicated that SKA1 may be a potential target protein for antiproliferative and anti‑invasive therapeutic strategies of human glioblastoma.
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Shi
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Xianzhen
authors.initials: X
authors.lastname: Chen
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Hu
authors.initials: H
authors.lastname: Peng
authors.affiliation: Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu 242000, P.R. China.
authors.forename: E
authors.initials: E
authors.lastname: Song
authors.affiliation: Medical Technology College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Xuancheng Central Hospital, Xuancheng, Anhui 242000, P.R. China.
authors.forename: Junxiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Xuancheng Central Hospital, Xuancheng, Anhui 242000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Tongji Hospital Affiliated to Tongji University, School of Medicine, Tongji University, Shanghai 200065, P.R. China.
authors.forename: Himaya
authors.initials: H
authors.lastname: Swa
authors.affiliation: Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608‑737, Republic of Korea.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608‑737, Republic of Korea.
authors.forename: Sekwon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000235:genetics*; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D034741:RNA, Small Interfering; D014161:Transduction, Genetic
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D034741:RNA, Small Interfering; C516427:SKA1 protein, human