title: The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
abstract: Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptors (TRAIL-R) are an important factor of apoptosis in cancer cells. There are no data about the effect of flavonols on the receptor expression on a surface of macrophage like cells. In this study, the expression level of TRAIL-R1 on murine RAW264.7 macrophages in the presence of selected flavonols: galangin, kaempferol, kaempferide and quercetin, which differ from their phenyl ring substituents, were studied. The expression of TRAIL-R1 death receptors on non-stimulated and lipopolysaccharide (LPS)-stimulated macrophages was determined using flow cytometry. The results suggested that compounds being tested can modulate TRAIL-R1 expression and can enhance TRAIL-mediated apoptosis.
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Chair and Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland. monika@warat.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Warat
authors.affiliation: School of Public Health in Bytom, Medical University of Silesia in Katowice, Toxicology and Drug Addiction Division, Communal Department of Hygiene and Sanitary Supervision, Medyków 18, 40-752 Katowice, Poland. tadsad@poczta.onet.pl.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Sadowski
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Chair and Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland. eszliszka@sum.edu.pl.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Szliszka
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Chair and Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland. wkrol@sum.edu.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Król
authors.affiliation: School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Chair and Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland. zczuba@sum.edu.pl.
authors.forename: Zenon P
authors.initials: ZP
authors.lastname: Czuba
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D044948:Flavonols / Q000737:chemistry / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000494:pharmacology
chemical_list: D044948:Flavonols; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D053221:TNF-Related Apoptosis-Inducing Ligand