title: Comparison of surface plasmon resonance, resonant waveguide grating biosensing and enzyme linked immunosorbent assay (ELISA) in the evaluation of a dengue virus immunoassay.
abstract: Two label-free biosensor platforms, Resonance Waveguide Grating (RWG) and Surface Plasmon Resonance (SPR), were used to rank a large panel of anti-dengue virus NS1 antibodies. Dengue non-structural 1 (NS1) protein is an established serological marker for the early detection of dengue infection. A variety of commercial dengue NS1 antigen capture immunoassays are available in both ELISA and lateral flow format. However, there is a significant scope to improve both the sensitivity and the specificity of those tests. The interactions of antibody (Ab)-antigen (Ag) were profiled, with weak interactions (KD = 1-0.1 μM) able to be detected under static equilibrium conditions by RWG, but not observed to under more rigorous flow conditions using SPR. There were significant differences in the absolute affinities determined by the two technologies, and there was a poor correlation between antibodies best ranked by RWG and the lower limit of detection (LLOD) found by ELISA. Hence, whilst high-throughput RWG can be useful as preliminary screening for higher affinity antibodies, care should be exercised in the assignation of quantitative values for affinity between different assay formats.
authors.affiliation: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072 Australia. zjyyhdm@163.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Hu
authors.affiliation: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072 Australia. scottfry@optusnet.com.au.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Fry
authors.affiliation: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072 Australia. j.huang2@uq.edu.au.
authors.forename: Johnny X
authors.initials: JX
authors.lastname: Huang
authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. dxx53542008@126.com.
authors.forename: Xixia
authors.initials: X
authors.lastname: Ding
authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. awen.qiu@163.com.
authors.forename: Liwen
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authors.lastname: Qiu
authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. pyx009@163.com.
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authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. redcellchen@163.com.
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authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. jingjin8211@yahoo.com.cn.
authors.forename: Jing
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authors.affiliation: Alere (Brisbane), 532 Seventeen Mile Rocks Road, Sinnamon Park, Qld, 4073 Australia. catriona.mcelnea@alere.com.
authors.forename: Catriona
authors.initials: C
authors.lastname: McElnea
authors.affiliation: Alere (San Diego), Summer Ridge Road, San Diego, CA 92121, USA. joseph.buechler@alere.com.
authors.forename: Joe
authors.initials: J
authors.lastname: Buechler
authors.affiliation: Centre for Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. chexiaoyan@126.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Che
authors.affiliation: Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072 Australia. m.cooper@uq.edu.au.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Cooper