title: Quantitative Relationships Between the Cytotoxicity of Flavonoids on the Human Breast Cancer Stem-Like Cells MCF7-SC and Their Structural Properties.
abstract: As some breast cancer-related deaths can be attributed to the metastasis of cancer stem cells, chemotherapeutic agents targeting breast cancer stem cells are of interest as a potential treatment. Flavonoids that exhibit cytotoxicity on breast cancer stem cells have rarely been observed. Thus, the objective of this study was to measure potential cytotoxic effects of 42 different flavonoids on the human breast cancer stem-like cell line, MCF7-SC. The relationship between flavonoid structural properties and cytotoxicity has not been reported previously; therefore, we determined quantitative structure-activity relationships using both comparative molecular field analysis and comparative molecular similarity analysis. Further biological experiments including Western blot analysis, flow cytometry, and immunofluorescence microscopy were also conducted on the most cytotoxic 8-chloroflavanone.
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Hyeryoung
authors.initials: H
authors.lastname: Jung
authors.affiliation: Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju, 690-756, Korea.
authors.forename: Soon Young
authors.initials: SY
authors.lastname: Shin
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Yearam
authors.initials: Y
authors.lastname: Jung
authors.affiliation: Department of Biochemical Engineering, Gangneung-Wonju National University, Gangwon, 210-702, Korea.
authors.forename: Thao Anh
authors.initials: TA
authors.lastname: Tran
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Hye Ok
authors.initials: HO
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Seoul, 136-714, Korea.
authors.forename: Kang-Yeoun
authors.initials: KY
authors.lastname: Jung
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Seoul, 136-714, Korea.
authors.forename: Dongsoo
authors.initials: D
authors.lastname: Koh
authors.affiliation: Department of Biochemical Engineering, Gangneung-Wonju National University, Gangwon, 210-702, Korea.
authors.forename: Somi Kim
authors.initials: SK
authors.lastname: Cho
authors.affiliation: Division of Bioscience and Biotechnology, BMIC, Konkuk University, Seoul, 143-701, Korea.
authors.forename: Yoongho
authors.initials: Y
authors.lastname: Lim
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003603:Cytotoxins / Q000737:chemistry / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells
chemical_list: D000970:Antineoplastic Agents; D003603:Cytotoxins; D005419:Flavonoids
keywords: 8-chloroflavanone; QSAR; cyclin-dependent kinase 6; flavonoid; flow cytometry; immunofluorescence microscopy; in silico docking