title: Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting.
abstract: Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min(-1)) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. The approach is generalizable to other microfluidic platforms for rapid, high-throughput analysis.
authors.affiliation: Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA. leor.weinberger@gladstone.ucsf.edu.
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Jung
authors.forename: Timothy
authors.initials: T
authors.lastname: Notton
authors.forename: Erika
authors.initials: E
authors.lastname: Fong
authors.forename: Maxim
authors.initials: M
authors.lastname: Shusteff
authors.forename: Leor S
authors.initials: LS
authors.lastname: Weinberger
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D006874:Hydrostatic Pressure; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D013601:T-Lymphocytes / Q000166:cytology*