title: Association of the rs10757274 SNP with coronary artery disease in a small group of a Pakistani population.
abstract: OBJECTIVE
  The present study aimed to investigate the association between the rs10757274 SNP (present on locus 9p21 in the gene for CDKN2B-AS1) and coronary artery disease (CAD) in a local population of Pakistan.
  
  
  METHODS
  It was a case-control study. An allele-specific PCR-based strategy was used for the identification of genotypes. A total of 350 samples were used for the investigation, out of which 220 samples were CAD patients and 130 samples were normal healthy individuals. Effects of parameters, like family history of CAD, smoking, presence of diabetes, and hypertension, in changing the chances of CAD were studied. Odds ratio was estimated with 95% confidence interval.
  
  
  RESULTS
  A strong association was observed between CAD and factors, like smoking (OR: 1.666; 95% CI: 1.042-2.664), presence of hypertension (OR: 26.55; 95% CI: 15.95-44.20), diabetes (OR: 3.009; 95% CI: 1.841-4.920), and family history of CAD (OR: 4.9; 95% CI: 2.965-8.099). Results for the association between the genotype on the basis of rs10757274 showed a strong association between the GG genotype and the occurrence of CAD (OR: 9.603; 95% CI: 5.746-16.05).
  
  
  CONCLUSIONS
  The present results suggest the importance of the 9p21 locus in modulating the chances of CAD.
authors.affiliation: Department of Biological Sciences, University of Sargodha; Sargodha-Pakistan. kashifnawazshabbir@yahoo.com.
authors.forename: Syed Kashif
authors.initials: SK
authors.lastname: Nawaz
authors.forename: Aasma
authors.initials: A
authors.lastname: Noreen
authors.forename: Asima
authors.initials: A
authors.lastname: Rani
authors.forename: Memoona
authors.initials: M
authors.lastname: Yousaf
authors.forename: Muhammad
authors.initials: M
authors.lastname: Arshad
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D003324:Coronary Artery Disease / Q000235:genetics* / Q000401:mortality; D050762:Cyclin-Dependent Kinase Inhibitor p15 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan; D020641:Polymorphism, Single Nucleotide
chemical_list: C493966:CDKN2B protein, human; D050762:Cyclin-Dependent Kinase Inhibitor p15