title: A novel IgM-H-ficolin complement pathway to attack allogenic cancer cells in vitro.
abstract: The pentameric serum IgMs are critical to immune defense and surveillance through cytotoxicity against microbes and nascent cancer cells. Ficolins, a group of oligomeric lectins with an overall structure similar to C1q and mannose-binding lectin (MBL) participate in microbe infection and apoptotic cell clearance by activating the complement lectin pathway or a primitive opsonophagocytosis. It remains unknown whether serum IgMs interplay with ficolins in cancer immunosurveillance. Here we report a natural cancer killing of different types of cancer cells by sera from a healthy human population mediated by a novel IgM-H-ficolin complement activation pathway. We demonstrate for the first time that H-ficolin bound to a subset of IgMs in positive human sera and IgM-H-ficolin deposited on cancer cells to activate complement attack in cancer cells. Our data suggest that the IgM-H-ficolin -mediated complement activation pathway may be another defensive strategy for human cancer immunosurveillance.
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Lei
authors.affiliation: Departments of Surgery and Urology, VA Boston Healthcare System, Boston University School of Medicine, Boston, MA 02130 USA.
authors.forename: Chaoxu
authors.initials: C
authors.lastname: Liu
authors.affiliation: Departments of Surgery and Urology, VA Boston Healthcare System, Boston University School of Medicine, Boston, MA 02130 USA.
authors.forename: Kazem
authors.initials: K
authors.lastname: Azadzoi
authors.affiliation: Cancer Research Center, School of Medicine, Shandong University, Jinan, 250000 China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Biochemistry and Molecular biology, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Fan
authors.initials: F
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: An
authors.initials: A
authors.lastname: Xiang
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Yanhai
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Qingchuan
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Cancer Biology, Department of Pharmacogenomics, School of Pharmacy, the Fourth Military Medical University, Xi'an, 710032 China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.affiliation: 1] Departments of Surgery and Urology, VA Boston Healthcare System, Boston University School of Medicine, Boston, MA 02130 USA [2] Cancer Research Center, School of Medicine, Shandong University, Jinan, 250000 China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D045744:Cell Line, Tumor; D003167:Complement Activation; D003165:Complement System Proteins / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D037102:Lectins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D061986:MCF-7 Cells; D008297:Male; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D011485:Protein Binding; D044967:Serum / Q000276:immunology / Q000378:metabolism
chemical_list: C109662:FCN3 protein, human; D006023:Glycoproteins; D007075:Immunoglobulin M; D037102:Lectins; D003165:Complement System Proteins