title: Syntheses, characterization, interaction with DNA, cytotoxic and apoptosis of two novel complexes of Zn(II) and Mn(II) with 2-methyl-1H-4,5-imidazoledicarboxylic acid.
abstract: Two new complexes, Zn(L)2(H2O)2 (1) and Mn(L)2(H2O)2 (2) [L = 2-Methyl-1H-4,5-imidazoledicarboxylic acid] were synthesized and characterized by elemental analysis, infrared spectroscopy, and single crystal X-ray diffraction. Intramolecular weak interactions, such as hydrogen-bond and intermolecular interactions were presented in the complexes. The activities of the complexes binding with DNA, and cytotoxic activities were studied. The binding of complexes with fish sperm DNA (FS-DNA) was investigated by fluorescence spectra. Gel electrophoresis assay demonstrated the ability of the complexes to cleave the pBR322 plasmid DNA. The cytotoxic activities of the complexes were tested against the KB cell line. Cytotoxic activity studies showed the two complexes exhibited significant cancer cell inhibitory rate. The most active compound was complex 1 with IC50 and CC50value of 36.5, 429, with the selectivity index (SI = 11.75) among the tested compounds.
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Ling-Feng
authors.initials: LF
authors.lastname: Li
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Chi
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Li
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Shi-Kai
authors.initials: SK
authors.lastname: Liang
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Jin
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Si-Jia
authors.initials: SJ
authors.lastname: Liu
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China.
authors.forename: Ming-Chang
authors.initials: MC
authors.lastname: Zhu
authors.affiliation: Department of Coordination Chemistry, International Key Laboratory of Shenyang, Inorganic Molecule-Based Chemical, Shenyang University of Chemical Technology, Shenyang 110142, China. Electronic address: enjungao@163.com.
authors.forename: En-Jun
authors.initials: EJ
authors.lastname: Gao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002264:Carboxylic Acids / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005399:Fishes; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D008297:Male; D008345:Manganese / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013094:Spermatozoa / Q000737:chemistry; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C000603212:2-methyl-1H-4,5-imidazoledicarboxylic acid; D000970:Antineoplastic Agents; D002264:Carboxylic Acids; D007093:Imidazoles; D009942:Organometallic Compounds; D008345:Manganese; D004247:DNA; D015032:Zinc
keywords: Cleavage; Cytotoxicity; Fluorescent; Hydrogen-bond