title: First isolation of enteroaggregative Escherichia coli O104:H4 from a diarrhea case in Argentina.
abstract: We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliCH4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 genes were included. Biochemical and antimicrobial susceptibility assays as well as serotyping were performed. The identified E. coli strain was susceptible to all antimicrobials tested and harbored the aggR, aaiC, pCVD432 plasmid, lpfO113, rfbO104, fliCH4 and terD genes. Although serotype EAEC O104:H4 rarely spreads and sporadic cases have been reported, global concern increased after the large-scale outbreak in Europe in 2011. The finding of EAEC O104:H4 reinforces the need for improved methodologies for the detection of all E. coli pathotypes.
authors.affiliation: Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina. Electronic address: ccarbonari@anlis.gov.ar.
authors.forename: Claudia C
authors.initials: CC
authors.lastname: Carbonari
authors.affiliation: Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Natalia
authors.initials: N
authors.lastname: Deza
authors.affiliation: Laboratorio de la Dirección de Patologías Prevalentes y Epidemiología, Ministerio de Salud de la Provincia de Chubut, Rawson, Provincia de Chubut, Argentina.
authors.forename: Mario
authors.initials: M
authors.lastname: Flores
authors.affiliation: Centro Periférico de Salud Ramón Carrillo, Hospital Zonal de Trelew, Trelew, Provincia de Chubut, Argentina.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Gasparini
authors.affiliation: Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Manfredi
authors.affiliation: Servicio Fisiopatogenia, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas - ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
authors.forename: Marta
authors.initials: M
authors.lastname: Rivas
mesh_terms: D001118:Argentina; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans
keywords: Diarrea; Diarrhea; Enteroaggregative Escherichia coli; Escherichia coli enteroagregativo; O104:H4 serotype; Serotipo O104:H4