title: p53-induced microRNA-1246 inhibits the cell growth of human hepatocellular carcinoma cells by targeting NFIB.
abstract: In recent years, miR-1246 has been identified as a transcriptional target of p53 in Down syndrome and may provide a new p53-miR-1246-DYRK1A-NFAT pathway in cancer. The present study aimed to explore the role of miR-1246 in the tumorigenesis of human hepatocellular carcinoma (HCC). We found that wild-type p53 regulated the expression of miR-1246 in HCC cell lines, and alteration of miR-1246 modulated cell proliferation, colony formation ability and apoptosis. The nuclear factor I/B (NFIB), an oncogene, was identified as a direct target gene of miR-1246 using a fluorescent reporter assay. Overexpression of NFIB abolished the regulation of cell apoptosis caused by miR-1246 in HepG2 cells. This finding suggests that miR-1246 is regulated by p53 and suppresses the growth of human HCC by targeting NFIB. Here, we propose a new p53-miR-1246-NFIB pathway in HCC.
authors.affiliation: Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Ultrasound, The Affiliated Hospital of Hebei University, Baoding, Hebei, P.R. China.
authors.forename: Li-Ye
authors.initials: LY
authors.lastname: Cao
authors.affiliation: Department of Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, P.R. China.
authors.forename: Shu-Jie
authors.initials: SJ
authors.lastname: Cheng
authors.affiliation: Department of Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, P.R. China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Zhang
authors.affiliation: Department of Surgery, The Affiliated Hospital of Hebei University, Baoding, Hebei, P.R. China.
authors.forename: Xiao-Shi
authors.initials: XS
authors.lastname: Jin
authors.affiliation: Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D051839:NFI Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53 / Q000235:genetics* / Q000378:metabolism
chemical_list: C577700:MIRN1246 microRNA, human; D035683:MicroRNAs; D051839:NFI Transcription Factors; C110754:NFIB protein, human; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53