title: Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold.
abstract: We introduce a new strategy for fabricating a seamless three-dimensional (3D) helical microreactor utilizing a silicone tube and a paraffin mold. With this method, various shapes and sizes of 3D helical microreactors were fabricated, and a complicated and laborious photolithographic process, or 3D printing, was eliminated. With dramatically enhanced portability at a significantly reduced fabrication cost, such a device can be considered to be the simplest microreactor, developed to date, for performing the flow-through polymerase chain reaction (PCR).
authors.affiliation: Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do 461-701, Korea. nylee@gachon.ac.kr.
authors.forename: Wenming
authors.initials: W
authors.lastname: Wu
authors.forename: Kieu The Loan
authors.initials: KT
authors.lastname: Trinh
authors.forename: Nae Yoon
authors.initials: NY
authors.lastname: Lee
mesh_terms: D004867:Equipment Design; D055616:Microtechnology / Q000379:methods*; D010232:Paraffin / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012828:Silicones / Q000737:chemistry*
chemical_list: D012828:Silicones; D010232:Paraffin