title: Immunohistochemical Characterization of Sympathetic Chain Ganglia (SChG) Neurons Supplying the Porcine mammary Gland.
abstract: The aim of this study was to investigate the chemical coding of mammary gland-projecting SChG neurons using double-labelling immunohistochemistry. Earlier observation showed that after injection of the retrograde tracer fast blue (FB) into the second, right thoracic mamma, FB+ mammary gland-projecting neurons were found in Th1-3, Th9-14 and L1-4 right SChG. The greatest number of FB+ nerve cell bodies was observed in Th10 (approx. 843) and Th11 (approx. 567). Neurons projecting to the last right abdominal mamma were found in L1-4 SChG. The greatest number of FB+ neurons was observed in L2 (approx. 1200). Immunohistochemistry revealed that the vast majority of FB+ mammary-projecting neurons contained immunoreactivities to TH (96.97%) and/or DßH (95.92%). Many TH/DßH-positive neurons stained for SOM (41.5%) or NPY (33.2%), and less numerous nerve cells expressed VIP (16.9%). This observation strongly corresponds to the results of previous studies concerning the immunohistochemical characterization of nerve fibres supplying the porcine mammary gland.
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Franke-Radowiecka
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: K
authors.initials: K
authors.lastname: Wąsowicz
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Klimczuk
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Podlasz
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Zalecki
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, Olsztyn, 10-719, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Sienkiewicz
mesh_terms: D059331:Adrenergic Neurons / Q000737:chemistry / Q000166:cytology; D000578:Amidines; D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods / Q000662:veterinary; D005456:Fluorescent Dyes; D005728:Ganglia, Sympathetic / Q000033:anatomy & histology / Q000737:chemistry / Q000166:cytology*; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry / Q000662:veterinary; D008321:Mammary Glands, Animal / Q000033:anatomy & histology / Q000294:innervation*; D051379:Mice; D009474:Neurons / Q000737:chemistry* / Q000145:classification / Q000166:cytology; D011817:Rabbits; D051381:Rats; D013552:Swine / Q000033:anatomy & histology*
chemical_list: D000578:Amidines; D005456:Fluorescent Dyes; D007106:Immune Sera; C031455:diamidino compound 253-50