title: Amelioration of oxidative stress by Tabernamontana divaricata on alloxan-induced diabetic rats.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the anti-diabetic activity of ethanol extract of Tabernamontana divaricata (L.) and its ameliorative effect on oxidative stress in alloxan-induced diabetic rats.
  
  
  METHODS
  Diabetes was induced by single intraperitoneal injection of alloxan monohydrate (140 mg/kg body weight). Methanol extract of T. divaricata was administered at the doses of 100 and 200 mg/kg body weight in diabetic induced rats including glibenclamide (3 mg/kg) as a reference drug. In the continuous 21 days treatment, fasting blood glucose level was determined on 0, 7, 14 and 21 days. On day 21, serum lipid profiles and glycosylated hemoglobin, liver antioxidant enzymes levels were estimated.
  
  
  RESULTS
  Experimental findings showed a significant anti-diabetic potential of the extract in terms of reduction in blood glucose levels and a correct effect on the altered biochemical parameters. Observed data were found statistically significant in correction of antioxidant enzyme level accompanied with diabetes, particularly at the dose of 200 mg/kg body weight.
  
  
  CONCLUSIONS
  Based on the results, it can be concluded that the T. divaricata is found to be effective in type 2 diabetes in rats and to have an ameliorative effect on the associated oxidative stress.
authors.affiliation: Department of Pharmacology, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Kanthlal
authors.affiliation: Department of Pharmacology, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India.
authors.forename: B Anil
authors.initials: BA
authors.lastname: Kumar
authors.affiliation: Department of Pharmacology, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India.
authors.forename: Jipnomon
authors.initials: J
authors.lastname: Joseph
authors.affiliation: Department of Pharmacognosy, Amrita School of Pharmacy, Amrita Viswa Vidyapeetham University, AIMS, Kochi, Kerala, India.
authors.forename: R
authors.initials: R
authors.lastname: Aravind
authors.affiliation: Department of Pharmacology, Erode College of Pharmacy, Erode, Tamil Nadu, India.
authors.forename: P Royal
authors.initials: PR
authors.lastname: Frank
keywords: Alloxan; Tabernamontana divaricata; anti-diabetic; antioxidant; methanol extract