title: Tenacity of human norovirus and the surrogates feline calicivirus and murine norovirus during long-term storage on common nonporous food contact surfaces.
abstract: The transfer of human norovirus (hNV) to food via contaminated surfaces is highly probable during food production, processing, and preparation. In this study, the tenacity of hNV and its cultivable surrogates feline calicivirus (FCV) and murine norovirus (MNV) on two common nonporous surface materials at two storage temperatures was directly compared. Virus titer reduction on artificially inoculated stainless steel and plastic carriers was monitored for 70 days at room temperature and at 7°C. Viruses were recovered at various time points by elution. Genomes from intact capsids (hNV, FCV, and MNV) were quantified with real-time reverse transcription (RT) PCR, and infectivity (FCV and MNV) was assessed with plaque assay. RNase treatment before RNA extraction was used to eliminate exposed RNA and to assess capsid integrity. No significant differences in titer reduction were found between materials (stainless steel or plastic) with the plaque assay or the real-time quantitative RT-PCR. At room temperature, infectious FCV and MNV were detected for 7 days. Titers of intact hNV, FCV, and MNV capsids dropped gradually and were still detectable after 70 days with a loss of 3 to 4 log units. At 7°C, the viruses were considerably more stable than they were at room temperature. Although only MNV infectivity was unchanged after 70 days, the numbers of intact capsids (hNV, FCV, and MNV) were stable with less than a 1-log reduction. The results indicate that hNV persists on food contact surfaces and seems to remain infective for weeks. MNV appears to be more stable than FCV at 7°C, and thus is the most suitable surrogate for hNV under dry conditions. Although a perfect quantitative correlation between intact capsids and infective particles was not obtained, real-time quantitative RT-PCR provided qualitative data about hNV inactivation characteristics. The results of this comparative study might support future efforts in assessment of foodborne virus risk and food safety.
authors.affiliation: Department of Life Science Technologies, Institute for Food Technology NRW, Division of Microbiology, Ostwestfalen-Lippe University of Applied Sciences, 32657 Lemgo, Germany. sascha.mormann@cvua-owl.de.
authors.forename: Sascha
authors.initials: S
authors.lastname: Mormann
authors.affiliation: Department of Life Science Technologies, Institute for Food Technology NRW, Division of Microbiology, Ostwestfalen-Lippe University of Applied Sciences, 32657 Lemgo, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Heißenberg
authors.affiliation: Department of Life Science Technologies, Institute for Food Technology NRW, Division of Microbiology, Ostwestfalen-Lippe University of Applied Sciences, 32657 Lemgo, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Pfannebecker
authors.affiliation: Department of Life Science Technologies, Institute for Food Technology NRW, Division of Microbiology, Ostwestfalen-Lippe University of Applied Sciences, 32657 Lemgo, Germany. barbara.becker@hs-owl.de.
authors.forename: Barbara
authors.initials: B
authors.lastname: Becker
mesh_terms: D000818:Animals; D017927:Calicivirus, Feline / Q000254:growth & development* / Q000472:pathogenicity; D002415:Cats; D005511:Food Handling / Q000379:methods*; D018857:Food Packaging; D061353:Food Storage / Q000379:methods*; D006801:Humans; D051379:Mice; D029322:Norovirus / Q000254:growth & development* / Q000472:pathogenicity; D010969:Plastics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013193:Stainless Steel; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D019562:Viral Load; D010948:Viral Plaque Assay
chemical_list: D010969:Plastics; D013193:Stainless Steel