title: [IDH1 mutation and MGMT expression in astrocytoma and the relationship with prognosis after radiotherapy].
abstract: OBJECTIVE
  To study the correlation between IDH1 mutation, MGMT expression, clinicopathologic features and post-radiotherapy prognosis in patients with astrocytoma.
  
  
  METHODS
  Detection of IDH1 mutation and MGMT expression was carried out in 48 cases of astrocytoma (WHO grade II to III) by EnVision method with immunohistochemical staining. Follow-up data, including treatment response and overall survival time, were analyzed.
  
  
  RESULTS
  The rates of IDH1 mutation and MGMT expression in astrocytomas were 62.7% (30/48) and 47.9% (23/48), respectively. There was a negative correlation between IDH1 mutation and MGMT expression (r = -0.641, P < 0.01). The age of patients with IDH1 mutation was younger at disease onset. The IDH1 mutation rate in patients with WHO grade II astrocytoma was higher than that in patients with WHO grade III tumor (P < 0.05). The age at onset was an independent factor affecting the expression of mutant IDH1. After radiotherapy, patients with IDH1 mutation+/MGMT- tumor carried a longer overall survival time than patients with IDH1 mutation-/MGMT+ tumor (P < 0.05).
  
  
  CONCLUSIONS
  There is a correlation between IDH1 mutation and MGMT expression in WHO grade II to III astrocytoma. Age at onset is an independent factor affecting the expression of mutant IDH1. Tumors with IDH1+/MGMT- pattern show better response to radiotherapy than tumors with IDH1-/MGMT+ pattern. Detection of IDH1 mutation and MGMT protein expression can provide some guidance in choice of treatment modalities in patients with astrocytoma.
authors.affiliation: Department of Pathology, Harbin Medical University First Hospital, Harbin 150001, China.
authors.forename: Mengwan
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Pathology, Harbin Medical University First Hospital, Harbin 150001, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Pathology, Harbin Medical University First Hospital, Harbin 150001, China.
authors.forename: Jiayao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Harbin Medical University First Hospital, Harbin 150001, China.
authors.forename: Jingqi
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Harbin Medical University First Hospital, Harbin 150001, China. E-mail: qijiping2003@163.com.
authors.forename: Jiping
authors.initials: J
authors.lastname: Qi
mesh_terms: D000328:Adult; D000367:Age Factors; D017668:Age of Onset; D000368:Aged; D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy; D015254:DNA Modification Methylases / Q000378:metabolism*; D045643:DNA Repair Enzymes / Q000378:metabolism*; D005260:Female; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D050505:Mutant Proteins / Q000235:genetics*; D009154:Mutation; D011379:Prognosis; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D025521:Tumor Suppressor Proteins; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes