title: Protective role of quercetin against cisplatin-induced hair cell damage in zebrafish embryos.
abstract: OBJECTIVE
  The aim of this study was to evaluate the protective effects of quercetin on cisplatin-induced hair cell damage in transgenic zebrafish embryos.
  
  
  METHODS
  Five days postfertilization zebrafish embryos were exposed to 1 mM cisplatin and quercetin at 10, 50, 100, or 200 μM for 4 h. Hair cells within neuromasts of the supraorbital, otic, and occipital lateral lines were analyzed by fluorescent microscopy (n = 10). Survival of hair cells was calculated as the average number of hair cells in the control group that were not exposed to cisplatin. Ultrastructural changes were evaluated using scanning electron microscopy.
  
  
  RESULTS
  Hair cell damage in neuromasts was decreased by co-treatment of quercetin and cisplatin (quercetin 100 μM: 8.6 ± 1.1 cells; 1 mM cisplatin only: 5.0 ± 0.5 cells; n = 10, p < 0.05); apoptosis of hair cells examined by special stain was also decreased by quercetin. The ultrastructure of hair cells within neuromasts was preserved in zebrafish by the combination of quercetin (100 μM) and cisplatin (1 mM).
  
  
  CONCLUSIONS
  In conclusion, quercetin showed protective effects against cisplatin-induced toxicity in a zebrafish model. The results of this study suggest the possibility of a protective role of quercetin against cisplatin-induced apoptotic cell death in zebrafish.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea.
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Oh
authors.affiliation: Laboratory of Neurodevelopmental Genetics, Graduate School of Medicine, Korea University, Ansan, Republic of Korea.
authors.forename: A Y
authors.initials: AY
authors.lastname: Chung
authors.affiliation: Laboratory of Neurodevelopmental Genetics, Graduate School of Medicine, Korea University, Ansan, Republic of Korea.
authors.forename: H C
authors.initials: HC
authors.lastname: Park
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Kwon
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Republic of Korea mednlaw@korea.ac.kr.
authors.forename: J
authors.initials: J
authors.lastname: Choi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D002945:Cisplatin / Q000009:adverse effects*; D004625:Embryo, Nonmammalian; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008928:Mitochondria / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D015027:Zebrafish
chemical_list: D000970:Antineoplastic Agents; D020011:Protective Agents; D011794:Quercetin; D002945:Cisplatin
keywords: Quercetin; cisplatin; hair cell; ototoxicity; zebrafish