title: [Differential expression of microRNA in chronic hepatitis B patients of pi-wei dampness-heat syndrome and of gan depression Pi deficiency syndrome: a primary research].
abstract: OBJECTIVE
  To explore different microRNA expression profiles between chronic hepatitis B (CHB) patients of Pi-Wei dampness-heat syndrome (PWDHS) and Gan depression Pi deficiency syndrome (GDPDS).
  
  
  METHODS
  By applying gene chip technology, blood samples from CHB patients of PWDHS (3 cases), GDPDS (3 cases), and healthy volunteers (3 cases) were withdrawn and microRNA detected. The microRNA was screened and functional analyses performed by using SAS system.
  
  
  RESULTS
  Totally 77 microRNAs with differential expression were screened from CHB patients of PWDHS and healthy volunteers, including 60 up-regulated microRNAs and 17 down-regulated microRNAs. Functions of target genes were mainly associated with transcription factors, gas exchange, adverse stimulating, regulation of enzyme activities, developing of the immune system, and the process of actin filaments. Totally 41 microRNAs with differential expression were screened from CHB patients of GDPDS and healthy volunteers, including 32 up-regulated microRNAs and 9 down-regulated microRNAs. Functions of target genes were mainly associated with binding to nucleotide or chromatin, inhibition and activation of transcription, biosynthesis, regulation of metabolic process, regulation of enzyme activities, developing of the immune system, the process of actin filaments, and IL-12. Totally 6 microRNAs with differential expression were screened from CHB patients of PWDHS and CHB patients of GDPDS, including 1 up-regulated microRNA and 5 down-regulated microRNAs. Functions of target genes were mainly associated with transmembrane transport, regulation of transcription factors, metabolism of hormones, developing of the immune system, the process of actin filaments, regulation of metabolic process, response to exterior stimulation, and so on.
  
  
  CONCLUSIONS
  There existed differentially expressed microRNAs (spectrum) between CHB patients of PWDHS and CHB patients of GDPDS.
authors.affiliation: Department of Gastroenterology, Pixian Chinese Medicine, Hospital, Sichuan, China.
authors.forename: En-Cheng
authors.initials: EC
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
authors.forename: Yin-Ling
authors.initials: YL
authors.lastname: Guo
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Cheng-Wei
authors.initials: CW
authors.lastname: He
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Shen
authors.forename: Quan-Sheng
authors.initials: QS
authors.lastname: Feng
mesh_terms: D003863:Depression; D019694:Hepatitis B, Chronic / Q000235:genetics / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D018664:Interleukin-12 / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D035683:MicroRNAs / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012106:Research; D013577:Syndrome
chemical_list: D035683:MicroRNAs; D018664:Interleukin-12