title: On the use of aptamer microarrays as a platform for the exploration of human prothrombin/thrombin conversion.
abstract: Microarrays are particular biosensors with multiple grafted probes that are generally used for parallel and simultaneous detection of various targets. In this study, we used microarrays with aptamer probes in order to follow up the different biomolecular interactions of a single enzyme, the thrombin protein, involved in the complex coagulation cascade. More precisely, thanks to label-free surface plasmon resonance imaging, we were able to monitor in real time an important step in the firing of the coagulation cascade in situ-the enzymatic transformation of prothrombin into thrombin, catalyzed by factor Xa. We were also able to appraise the influence of other biochemical factors and their corresponding inhibiting or enhancing behaviors on thrombin activation. Our study opens the door for the development of a complete microarray-based platform not only for the whole coagulation cascade analysis but also for novel drug screening assays in pharmacology.
authors.affiliation: Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France; Centre National de la Recherche Scientifique (CNRS), SPrAM, F-38000 Grenoble, France; Centre d'Etudes Atomiques (CEA), INAC-SPrAM, F-38000 Grenoble, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Daniel
authors.affiliation: Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France; Centre National de la Recherche Scientifique (CNRS), SPrAM, F-38000 Grenoble, France; Centre d'Etudes Atomiques (CEA), INAC-SPrAM, F-38000 Grenoble, France. Electronic address: yoann.roupioz@cea.fr.
authors.forename: Yoann
authors.initials: Y
authors.lastname: Roupioz
authors.affiliation: Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France; Centre National de la Recherche Scientifique (CNRS), SPrAM, F-38000 Grenoble, France; Centre d'Etudes Atomiques (CEA), INAC-SPrAM, F-38000 Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
authors.affiliation: Université Grenoble Alpes, INAC-SPrAM, F-38000 Grenoble, France; Centre National de la Recherche Scientifique (CNRS), SPrAM, F-38000 Grenoble, France; Centre d'Etudes Atomiques (CEA), INAC-SPrAM, F-38000 Grenoble, France.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Buhot
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D004353:Drug Evaluation, Preclinical; D006801:Humans; D046228:Microarray Analysis / Q000379:methods*; D011516:Prothrombin / Q000378:metabolism*; D013917:Thrombin / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D011516:Prothrombin; D013917:Thrombin
keywords: Aptamers; Coagulation cascade; Microarrays; Prothrombin; Surface plasmon resonance imaging; Thrombin