title: Poly (N-isopropylacrylamide)-co-(acrylic acid) microgel/Ag nanoparticle hybrids for the colorimetric sensing of H2O2.
abstract: Poly (N-isopropylacrylamide)-co-(acrylic acid) (pNIPAm-co-AAc) microgels composed of Ag nanoparticles (Ag NPs) have been synthesized and employed for the colorimetric sensing of H2O2. Each pNIPAm-co-AAc microgel, which exhibited a diameter of ∼800 nm, contained multiple Ag NPs (diameter of ∼5 nm), and solutions of these hybrid materials showed a UV-vis absorption band at ∼400 nm. This is due to the excitation of the Ag NP surface plasmon. We go on to show that the intensity of this absorption band is dependent on the concentration of H2O2 in solution. Specifically, in the presence of H2O2 the magnitude of the absorption peak dramatically decreases in a linear fashion over the concentration range of 0.30 to 3.00 μM H2O2 (r(2) = 0.9918). We go on to show that the response is selective for H2O2 and can still function in complex mixtures, e.g., we showed that the response is still robust in milk samples. While Ag NPs themselves can exhibit similar responses, this system has many benefits including sample processing and long term stability - i.e., Ag NPs are destabilized in solutions of a certain pH, and aggregate readily. Our microgel/Ag NP hybrids have been shown to be extremely stable and are easily purified prior to use by simple centrifugation/washing protocols. This system is simple and straightforward to use, is low cost, and can be used in complex media, which makes it practical for analyzing complex biological and environmental samples.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2. michael.serpe@ualberta.ca hdm@tzc.edu.cn.
authors.forename: De-Man
authors.initials: DM
authors.lastname: Han
authors.forename: Qiang Matthew
authors.initials: QM
authors.lastname: Zhang
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Serpe
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000179:Acrylates / Q000737:chemistry*; D000818:Animals; D003124:Colorimetry / Q000379:methods*; D004563:Electrochemistry; D004784:Environmental Monitoring; D005504:Food Analysis; D005782:Gels; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008892:Milk; D036103:Nanotechnology; D011786:Quality Control; D012834:Silver / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance
chemical_list: D000178:Acrylamides; D000179:Acrylates; D005782:Gels; D012834:Silver; C067295:N-isopropylacrylamide; D006861:Hydrogen Peroxide; C036658:acrylic acid