title: Quantitative assay for the colonization ability of heterogeneous bacteria on controlled nanopillar structures.
abstract: The colonization ability of bacteria on biomaterial surfaces is influenced by the morphology of the bacteria and the nanotopography of the biomaterial. However, interactions between the bacterial morphology and nanotopography of biomaterials have not yet been completely elucidated. In this article, we quantitatively characterized the bacterial morphology to illuminate the integrated effects of polyethylene terephthalate (PET) nanopillar arrays on the colonization of bacteria cells with different shapes. Our results demonstrated that the interaction between interpillar spacing and the diameter of the bacterial cells impacted the number of bacterial cells that adhered to different PET substrates. The interpillar spacing of nanopillar arrays promotes bacterial adhesion in a definite range (<50 nm). However, further increasing the interpillar spacing inhibited the adhesion of bacteria to the nanopillar arrays. Moreover, the interpillar spacing also influenced the morphologies of adherent bacterial cells on the PET nanopillar arrays, which consequently facilitated bacterial adhesion to the nanopillar arrays. Our findings enhance the understanding of interactions between controlled nanotopography and bacterial colonization and provide an appropriate parameter for the design of antibacterial materials with nanotopography.
authors.affiliation: Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China. National Center for Nanoscience and Technology, Beijing, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Jin
authors.forename: Wen
authors.initials: W
authors.lastname: Guo
authors.forename: Peihong
authors.initials: P
authors.lastname: Xue
authors.forename: Hainan
authors.initials: H
authors.lastname: Gao
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Chen
authors.initials: C
authors.lastname: Zheng
authors.forename: Yali
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dong
authors.initials: D
authors.lastname: Han
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000648:ultrastructure; D001422:Bacterial Adhesion; D008422:Materials Testing; D049329:Nanostructures / Q000737:chemistry*; D011093:Polyethylene Terephthalates / Q000737:chemistry*
chemical_list: D011093:Polyethylene Terephthalates