title: Lactonase activity and status of paraoxonase 1 in Chinese women with polycystic ovarian syndrome.
abstract: OBJECTIVE
  To study the relationship between the lactonase activities and status of paraoxonase 1 (PON1) and its association with the PON1 genetic polymorphisms in women with polycystic ovarian syndrome (PCOS).
  
  
  METHODS
  A case-control study.
  
  
  METHODS
  A total of 455 PCOS patients and 441 control women were included in this study. The lactonase activities and concentrations of PON1 were assayed using 5-thiobutyl butyrolactone (TBBL) and 7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin (DEPCyMC) respectively. A normalized lactonase activity (NLA) was estimated based on the ratio of TBBLase:DEPCyMCase activity. The PON1 genotypes, serum malondialdehyde (MDA) levels and total antioxidant capacity were analyzed.
  
  
  RESULTS
  The lactonase activities and levels of PON1 were higher in PCOS patients than in the control women. However, the NLA did not significantly differ between groups. The -108C→T variation of the PON1 gene showed decreased lactonase activities and levels of PON1 in a genotype-dependent manner (CC>CT>TT); the 192Q→R variation of the PON1 gene showed increased PON1 lactonase activities and NLA; and the 55L→M variation of the PON1 gene showed decreased lactonase activities and levels of PON1 but an increased NLA. A multivariable regression analysis showed that the -108C/T, 192Q/R, and 55L/M variations of the PON1 gene, serum apolipoprotein A1, and MDA levels were significant predictors of PON1 lactonase activity, PON1 level, and NLA.
  
  
  CONCLUSIONS
  The serum lactonase activities and concentrations of PON1 are increased in PCOS patients. The increased oxidative stress and the -108C/T, 192Q/R, and 55L/M genetic polymorphisms of PON1 may be associated with these changes.
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Kelei
authors.initials: K
authors.lastname: Xu
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Huai
authors.initials: H
authors.lastname: Bai
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Obstetrics and GynecologyWest China Second University HospitalWest China School of PharmacyLaboratory of Genetic Disease and Perinatal Medicine and Key Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of EducationWest China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, People's Republic of China fanping15@scu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Fan
mesh_terms: D000328:Adult; D043303:Aryldialkylphosphatase / Q000235:genetics* / Q000378:metabolism*; D044466:Asian People / Q000235:genetics; D002265:Carboxylic Ester Hydrolases; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007783:Lactones / Q000378:metabolism*; D010755:Organophosphates / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D010641:Phenotype; D011085:Polycystic Ovary Syndrome / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D014468:Umbelliferones / Q000378:metabolism*; D055815:Young Adult
chemical_list: C523443:7-O-diethylphosphoryl-3-cyano-4-methyl-7-hydroxycoumarin; D007783:Lactones; D010755:Organophosphates; D014468:Umbelliferones; D002265:Carboxylic Ester Hydrolases; C020579:gluconolactonase; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human