title: Biodiesel production from microalgae oil catalyzed by a recombinant lipase.
abstract: A recombinant Rhizomucor miehei lipase was constructed and expressed in Pichia pastoris. The target enzyme was termed Lipase GH2 and it can be used as a free enzyme for catalytic conversion of microalgae oil mixed with methanol or ethanol for biodiesel production in an n-hexane solvent system. Conversion rates of two major types of biodiesel, fatty acid methyl ester (FAME) and fatty acid ethyl ester (FAEE), reached maximal values (>90%) after 24h. The process of FAME production is generally more simple and economical than that of FAEE production, even though the two processes show similar conversion rates. In spite of the damaging effect of ethanol on enzyme activity, we successfully obtained ethyl ester by the enzymatic method. Our findings indicate that Lipase GH2 is a useful catalyst for conversion of microalgae oil to FAME or FAEE, and this system provides efficiency and reduced costs in biodiesel production.
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Ji
authors.initials: J
authors.lastname: Xia
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China. Electronic address: yingli528@vip.sina.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratories for Agro-biotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
authors.forename: Jilun
authors.initials: J
authors.lastname: Li
mesh_terms: D056804:Biofuels; D001709:Biotechnology / Q000379:methods*; D005591:Chemical Fractionation; D048408:Chlorella vulgaris / Q000378:metabolism*; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D058086:Microalgae / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013696:Temperature
chemical_list: D056804:Biofuels; D010938:Plant Oils; D011994:Recombinant Proteins; D008049:Lipase
keywords: Biodiesel; Conversion efficiency; Esterification reaction; Microalgae oil; Recombinant lipase