title: The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L.
abstract: The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.
authors.affiliation: Dept. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: marek.kamlar@gmail.com.
authors.forename: Marek
authors.initials: M
authors.lastname: Kamlar
authors.affiliation: Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: olga.rothova@natur.cuni.cz.
authors.forename: Olga
authors.initials: O
authors.lastname: Rothova
authors.affiliation: Dept. of Natural Products, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic; Present address: Biomedical Research Center, University Hospital Hradec Kralove, 500 05 Hradec Kralove, Czech Republic. Electronic address: salajkova.kac@gmail.com.
authors.forename: Sarka
authors.initials: S
authors.lastname: Salajkova
authors.affiliation: Laboratory of Growth Regulators, Palacky Univ. and Institute of Experimental Botany ASCR, Olomouc 78371, Czech Republic. Electronic address: tarkowska@ueb.cas.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Tarkowska
authors.affiliation: Dept. of Natural Products, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: Pavel.Drasar@vscht.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Drasar
authors.affiliation: Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: kocova@natur.cuni.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Kocova
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, Prague 16610, Czech Republic. Electronic address: harmatha@uochb.cas.cz.
authors.forename: Juraj
authors.initials: J
authors.lastname: Harmatha
authors.affiliation: Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic. Electronic address: dana.hola@natur.cuni.cz.
authors.forename: Dana
authors.initials: D
authors.lastname: Hola
authors.affiliation: Institute of Organic Chemistry and Biochemistry ASCR, Prague 16610, Czech Republic. Electronic address: ladislavkohout@seznam.cz.
authors.forename: Ladislav
authors.initials: L
authors.lastname: Kohout
authors.affiliation: Dept. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic. Electronic address: tomas.macek@vscht.cz.
authors.forename: Tomas
authors.initials: T
authors.lastname: Macek
mesh_terms: D060406:Brassinosteroids / Q000494:pharmacology*; D026461:Ecdysteroids / Q000378:metabolism*; D008968:Molecular Conformation; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism*; D018724:Spinacia oleracea / Q000737:chemistry* / Q000187:drug effects; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
chemical_list: D060406:Brassinosteroids; D026461:Ecdysteroids; D013260:Steroids, Heterocyclic; C023623:brassinolide
keywords: 20-Hydroxyecdysone; 24-Epibrassinolide; Ecdysteroids; Spinach