title: Effect of estradiol on apoptosis, proliferation and steroidogenic enzymes in the testes of the toad Rhinella arenarum (Amphibia, Anura).
abstract: Estrogens inhibit androgen production and this negative action on amphibian steroidogenesis could be related to the regulation of steroidogenic enzymes. Estrogens are also involved in the regulation of amphibian spermatogenesis by controlling testicular apoptosis and spermatogonial proliferation. The Bidder's organ (BO) is a structure characteristic from the Bufonidae family and in adult males of Rhinella arenarum it is one of the main sources of plasma estradiol (E2). The purpose of this study is to analyze the effect of E2 on testicular steroidogenic enzymes, apoptosis and proliferation in the toad R. arenarum. For this purpose, testicular fragments were treated during 24h with or without 2 or 20nM of E2. After treatments, the activities of cytochrome P450 17α-hydroxylase-C17-20 lyase (CypP450c17) and 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD/I) were measured by the transformation of radioactive substrates into products, and CypP450c17 expression was determined by Western blot analysis. Apoptosis in testicular sections was detected with a commercial fluorescent kit based on TUNEL method, and proliferation was evaluated by BrdU incorporation. Results indicate that E2 has no effect on CypP450c17 protein levels or enzymatic activity, while it reduces 3β-HSD/I activity during the post reproductive season. Furthermore, although E2 has no effect on apoptosis during the pre and the post reproductive seasons, it stimulates testicular apoptosis during the reproductive season, mostly in spermatocytes. Finally, E2 has no effect on testicular proliferation all year long. Taken together, these results suggest that E2 is involved in the regulation of testicular steroidogenesis and spermatogenesis.
authors.affiliation: Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: mflorenciascaia@bg.fcen.uba.ar.
authors.forename: María Florencia
authors.initials: MF
authors.lastname: Scaia
authors.affiliation: Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: claravolonteri@bg.fcen.uba.ar.
authors.forename: María Clara
authors.initials: MC
authors.lastname: Volonteri
authors.affiliation: Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: czuchlej@bg.fcen.uba.ar.
authors.forename: Silvia Cristina
authors.initials: SC
authors.lastname: Czuchlej
authors.affiliation: Laboratorio de Endocrinología Comparada, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina. Electronic address: nceballo@bg.fcen.uba.ar.
authors.forename: Nora Raquel
authors.initials: NR
authors.lastname: Ceballos
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D015096:3-Hydroxysteroid Dehydrogenases / Q000378:metabolism; D000728:Androgens / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001141:Aromatase / Q000378:metabolism*; D002022:Bufo arenarum / Q000254:growth & development / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D011863:Radioimmunoassay; D012098:Reproduction / Q000502:physiology; D013091:Spermatogenesis / Q000187:drug effects / Q000502:physiology*; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000728:Androgens; D004958:Estradiol; D015067:17-Hydroxysteroid Dehydrogenases; D015096:3-Hydroxysteroid Dehydrogenases; C044256:3 (or 17)-beta-hydroxysteroid dehydrogenase; D001141:Aromatase; D013254:Steroid 17-alpha-Hydroxylase
keywords: Apoptosis; Estradiol; Proliferation; Steroidogenesis; Testes; Toad