title: CYANOCHIP: an antibody microarray for high-taxonomical-resolution cyanobacterial monitoring.
abstract: Cyanobacteria are Gram-negative photosynthetic prokaryotes that are widespread on Earth. Eutrophication and global warming make some aquatic ecosystems behave as bioreactors that trigger rapid and massive cyanobacterial growth with remarkable economic and health consequences. Rapid and efficient early warning systems are required to support decisions by water body authorities. We have produced 17 specific antibodies to the most frequent cyanobacterial strains blooming in freshwater ecosystems, some of which are toxin producers. A sandwich-type antibody microarray immunoassay (CYANOCHIP) was developed for the simultaneous testing of any of the 17 strains, or other closely related strains, in field samples from different habitats (water, rocks, and sediments). We titrated and tested all of the antibodies in succession using a fluorescent sandwich microarray immunoassay. Although most showed high specificity, we applied a deconvolution method based on graph theory to disentangle the few existing cross-reactions. The CYANOCHIP sensitivity ranged from 10(2) to 10(4) cells mL(-1), with most antibodies detecting approximately 10(2) cells mL(-1). We validated the system by testing multiple isolates and crude natural samples from freshwater reservoirs and rocks, both in the laboratory and by in situ testing in the field. The results demonstrated that CYANOCHIP is a valuable tool for the sensitive and reliable detection of cyanobacteria for early warning and research purposes.
authors.affiliation: Department of Molecular Evolution, Centro de Astrobiología (INTA-CSIC) , Carretera de Ajalvir km 4, Torrejón de Ardoz, 28850 Madrid, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Blanco
authors.forename: Antonio
authors.initials: A
authors.lastname: Quesada
authors.forename: Ignacio
authors.initials: I
authors.lastname: Gallardo-Carreño
authors.forename: Jacobo
authors.initials: J
authors.lastname: Aguirre
authors.forename: Victor
authors.initials: V
authors.lastname: Parro
mesh_terms: D000906:Antibodies; D000458:Cyanobacteria / Q000145:classification / Q000276:immunology; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods; D005068:Eutrophication; D005618:Fresh Water / Q000382:microbiology*; D007118:Immunoassay / Q000379:methods; D046228:Microarray Analysis / Q000379:methods*; D015203:Reproducibility of Results; D013030:Spain; D014871:Water Microbiology
chemical_list: D000906:Antibodies