title: Involvement of NEDD9 in the invasion and migration of gastric cancer.
abstract: Recent studies have demonstrated that neural precursor cell expressed, developmentally downregulated 9 (NEDD9) is highly expressed in various tumor tissues and cell lines. However, research on the role of NEDD9 in gastric cancer (GC) is rare, and the potential mechanism in tumor progression has not yet been explored. In this study, we investigated the role and mechanism of NEDD9 in GC. The expression of NEDD9 in GC tissues and cell lines was measured by immunohistochemistry, qRT-PCR, and Western blot, respectively. Inhibiting NEDD9 expression was carried out by siRNA transfection, and upregulating of NEDD9 was via NEDD9 overexpression plasmid. The ability of proliferation, migration, and invasion was detected by MTT assay, scratch wound assay, and transwell assay, respectively. The expression of vimentin, E-cadherin, Zeb1, and Zeb2 was measured by Western blot and qRT-PCR. We found that NEDD9 expression was dramatically increased both in GC tissues and cell lines, and the expression was significantly related to GC development. Knockdown of NEDD9 in SGC-7901 strongly inhibited its malignant capacity in vitro. Meanwhile, upregulation of NEDD9 in GES-1 increased the malignant capacity. In addition, the expression of vimentin, Zeb1, and Zeb2 was positively correlated with NEDD9, while E-cadherin was opposite. Collectively, our findings suggest that NEDD9 acts as an oncogene and promotes GC metastasis via EMT.
authors.affiliation: Department of General Surgery, The First People's Hospital of Changzhou, the Third Affiliated Hospital of Soochow University, Changzhou, 213000, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Feng
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Zhao
authors.forename: Haibin
authors.initials: H
authors.lastname: Xie
authors.forename: Yong
authors.initials: Y
authors.lastname: Yin
authors.forename: Guanghua
authors.initials: G
authors.lastname: Luo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Feng
authors.forename: Zhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis* / Q000235:genetics; D000368:Aged; D017209:Apoptosis / Q000235:genetics; D015820:Cadherins; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D015820:Cadherins; C099942:NEDD9 protein, human; D010750:Phosphoproteins