title: In vitro antibiotic susceptibilities of Yersinia pestis determined by broth microdilution following CLSI methods.
abstract: In vitro susceptibilities to 45 antibiotics were determined for 30 genetically and geographically diverse strains of Yersinia pestis by the broth microdilution method at two temperatures, 28°C and 35°C, following Clinical and Laboratory Standards Institute (CLSI) methods. The Y. pestis strains demonstrated susceptibility to aminoglycosides, quinolones, tetracyclines, β-lactams, cephalosporins, and carbapenems. Only a 1-well shift was observed for the majority of antibiotics between the two temperatures. Establishing and comparing antibiotic susceptibilities of a diverse but specific set of Y. pestis strains by standardized methods and establishing population ranges and MIC50 and MIC90 values provide reference information for assessing new antibiotic agents and also provide a baseline for use in monitoring any future emergence of resistance.
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA henry.heine@medicine.ufl.edu.
authors.forename: Henry S
authors.initials: HS
authors.lastname: Heine
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Hershfield
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Charles
authors.initials: C
authors.lastname: Marchand
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Lynda
authors.initials: L
authors.lastname: Miller
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Halasohoris
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Bret K
authors.initials: BK
authors.lastname: Purcell
authors.affiliation: Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Worsham
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods / Q000592:standards; D010930:Plague / Q000382:microbiology; D013696:Temperature; D015010:Yersinia pestis / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents