title: The effects of baicalin on the TLR2/4 signaling pathway in the peripheral blood mononuclear cells of a lipopolysaccharide-induced rat fever model.
abstract: Baicalin, which is a flavonoid compound isolated from Scutellariae radix, has significant antipyretic effects. The aim of the present study was to evaluate the effects of baicalin on the Toll-like receptor 2/4 (TLR2/4) signaling pathway in the peripheral blood mononuclear cells (PBMCs) of a rat fever model induced by lipopolysaccharide (LPS). Sprague-Dawley rats were injected intraperitoneally with 100μg/kg LPS with or without a 160mg/kg baicalin treatment to induce fever. The results showed that baicalin significantly reduced the body temperatures of the fever-induced rats, inhibited the LPS-modulated upregulation of TLR4 mRNA and protein expression and TNF-α and IL-1β mRNA expression in the rat PBMCs and downregulated nuclear factor-κB activation with simultaneous decreases in TNF-α and IL-1β protein expression. The LPS and baicalin had no significant effect on TLR2 mRNA or protein expression in the PBMCs. These data suggest that baicalin can inhibit the TLR4 signaling pathway in the PBMCs of our animal model. Our findings may provide new mechanistic insights into the antipyretic effects of baicalin.
authors.affiliation: Department of Pediatrics, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041,PR China; Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry Education, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Pediatrics, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041,PR China. Electronic address: hxtyh@sina.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Tao
authors.affiliation: Department of Pediatrics, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041,PR China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry Education, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041, PR China.
authors.forename: Ting
authors.initials: T
authors.lastname: Feng
authors.affiliation: Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry Education, West China Second University Hospital, Sichuan University, No. 20, Section 3, Renmin Nan Lu, Chengdu, Sichuan 610041, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D058633:Antipyretics / Q000008:administration & dosage*; D001831:Body Temperature / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005334:Fever / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology; D005419:Flavonoids / Q000008:administration & dosage*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D008516:Medicine, Chinese Traditional; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis / Q000276:immunology*; D015398:Signal Transduction / Q000187:drug effects; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D058633:Antipyretics; D005419:Flavonoids; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D016328:NF-kappa B; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin
keywords: Antipyretic effect; Baicalin; Peripheral blood mononuclear cells; Signaling pathway; Toll-like receptor 2; Toll-like receptor 4