title: Antinociceptive effect of hydantoin 3-phenyl-5-(4-ethylphenyl)-imidazolidine-2,4-dione in mice.
abstract: Imidazolidine derivatives, or hydantoins, are synthetic compounds with different therapeutic applications. Many imidazolidine derivatives have psychopharmacological properties, such as phenytoin, famous for its anticonvulsant efficacy, but also effective in the treatment of neuropathic pain. The hydantoin, 3-phenyl-5-(4-ethylphenyl)-imidazolidine-2,4-dione (IM-3), synthesized from the amino acid, glycine, was selected for psychopharmacological studies in mice on the basis of its chemical and structural similarity with phenytoin. The first step of this study was to define the LD50, which determined the doses of 50, 100 and 200 mg/kg for subsequent tests. The results obtained from the behavioral screening indicated that IM-3 produces decreased ambulation and analgesia in mice. Motor coordination and anxiety behavior were not affected by treatment with IM-3, as observed in the rotarod and elevated plus-maze tests, respectively. Regarding its antinociceptive properties, IM-3 showed efficacy in the acetic acid-induced writhing test by increasing the latency of the first writhe and reducing the number of writhes, as well as reducing the paw licking time in the second phase of the formalin test. The behavior of treated animals exposed to the hot plate test, however, did not differ from that of the control group. These data suggest that IM-3 has antinociceptive effects in mice, which is probably mediated by anti-inflammatory mechanisms.
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. qronaldo@ig.com.br.
authors.forename: Ronaldo Bezerra
authors.initials: RB
authors.lastname: de Queiroz
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. fabiolalelis@ltf.ufpb.br.
authors.forename: Fabíola Lélis
authors.initials: FL
authors.lastname: de Carvalho
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. divilar@hotmail.com.
authors.forename: Diogo Vilar da
authors.initials: DV
authors.lastname: Fonsêca
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. jbarbosa@ltf.ufpb.br.
authors.forename: José Maria
authors.initials: JM
authors.lastname: Barbosa-Filho
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. paulasalgado87@gmail.com.
authors.forename: Paula Regina Rodrigues
authors.initials: PR
authors.lastname: Salgado
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. liana@ccs.ufpb.br.
authors.forename: Luciano Leite
authors.initials: LL
authors.lastname: Paulo
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. bmqueiroz1@gmail.com.
authors.forename: Ana Bárbara Maroja
authors.initials: AB
authors.lastname: de Queiroz
authors.affiliation: Center for Health Sciences, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. liana@ccs.ufpb.br.
authors.forename: Liana Clébia de Morais
authors.initials: LC
authors.lastname: Pordeus
authors.affiliation: Department of Chemistry, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. sasindustrial@bol.com.br.
authors.forename: Severino Araújo
authors.initials: SA
authors.lastname: de Souza
authors.affiliation: Department of Chemistry, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. helivaldog3@gmail.com.
authors.forename: Helivaldo Diogenes da Silva
authors.initials: HD
authors.lastname: Souza
authors.affiliation: Department of Chemistry, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. brunofrlira@hotmail.com.
authors.forename: Bruno Freitas
authors.initials: BF
authors.lastname: Lira
authors.affiliation: Department of Chemistry, Federal University of Paraiba, João Pessoa PB 58059-900, Brazil. athayde-filho@quimica.ufpb.br.
authors.forename: Petrônio Filgueiras
authors.initials: PF
authors.lastname: de Athayde-Filho
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal; D005557:Formaldehyde; D006827:Hydantoins / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D045442:Rotarod Performance Test; D023382:Toxicity Tests, Acute
chemical_list: D000700:Analgesics; D006827:Hydantoins; C000601100:hydantoin 3-phenyl-5-(4-ethylphenyl)-imidazolidine-2,4-dione; D005557:Formaldehyde; D019342:Acetic Acid