title: Retaining the long-survive capacity of Circulating Tumor Cells (CTCs) followed by xeno-transplantation: not only from metastatic cancer of the breast but also of prostate cancer patients.
abstract: We investigated whether Circulating Tumor Cells (CTCs) isolated from epithelial tumors could survive and grow in xenotransplants. To this purpose, EpCAM-positive CTCs were enriched by CellSearch platform the only FDA-cleared automated platform that quantifies tumor burden in peripheral blood and provides clinical evidence of predictive and prognostic value. The CTCs were isolated from metastatic prostate (n=6) and breast (n=2) cancer patients. The xenograft assay was developed in 8-week-old NOD/SCID mice that were subcutaneously injected with increasing amounts of CTCs (ranging from 50 to 3000). Human CTCs were found in 8 out of 8 murine peripheral blood (muPB) and in 6 out of 8 murine bone marrow (muBM) samples, after a median follow-up of 10.3 months. Six out of 8 spleens were positive for human cytokeratin. Our assay showed higher successful rate than those previously reported in breast cancer and hepatocellular carcinoma. The role of EpCAM during carcinogenesis is controversial. The identification of human CTCs in muPB, muBM and spleen demonstrates that the EpCAM-positive fraction of CTCs retains the migratory capacity. This is the first experimental evidence that as few as 50 EpCAM-positive prostate cancer CTCs putatively contain metastasis-initiating-cells (MIC).
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, Oncology Section, University of Padova, Padova, Italy.|IOV-IRCCS, Padova, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Rossi
authors.affiliation: Department of Medical Diagnostic Sciences & Special Therapies, Surgical Pathology & Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Rugge
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, Oncology Section, University of Padova, Padova, Italy.|IOV-IRCCS, Padova, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Facchinetti
authors.affiliation: Department of Medical Diagnostic Sciences & Special Therapies, Surgical Pathology & Cytopathology Unit, University of Padova, Padova, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Pizzi
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Nardo
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, Oncology Section, University of Padova, Padova, Italy.
authors.forename: Vito
authors.initials: V
authors.lastname: Barbieri
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Manicone
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: De Faveri
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Chiara Scaini
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Umberto
authors.initials: U
authors.lastname: Basso
authors.affiliation: Department of Surgery, Oncology and Gastroenterology, Oncology Section, University of Padova, Padova, Italy.|IOV-IRCCS, Padova, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Amadori
authors.affiliation: IOV-IRCCS, Padova, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Zamarchi
keywords: Circulating Tumor Cells; EpCAM; breast cancer; prostate cancer; xenograft assay