title: Preparation of linear maltodextrins using a hyperthermophilic amylopullulanase with cyclodextrin- and starch-hydrolysing activities.
abstract: A novel method for the preparation of linear maltodextrins from cyclodextrins and starch was proposed. To accomplish this process, an amylopullulanase from hyperthermophilic archaeon Caldivirga maquilingensis (CMApu) was characterized and used. CMApu with an estimated molecular mass of 62.7 kDa by SDS-PAGE had a maximal pullulan-hydrolysing activity at 100°C and pH 5.0. It could also hydrolyse amylopectin (AP), starch, β-CD and amylose (AM), in a decreasing order of relative activities from 88.96% to 57.17%. TLC and HPAEC analysis revealed that CMApu catalyzed the debranching and degrading reactions to produce linear malto-oligosaccharides (≤ G8-G1) from G8-β-CD and/or normal CDs, amylodextrins (DP6-96) from AM, and amylodextrins (DP1-76) from AP and potato starch. Our results showed that CMApu had a great potential for the industrial preparation of linear maltodextrins from normal starch instead of waxy starch, malto-oligosaccharides or sucrose. And the high optimal temperature of CMApu facilitated the simultaneous gelatinization and hydrolysis of cereal starch.
authors.affiliation: Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, and College of Food Science and Engineering, Changchun University, Satellite Road 6543, Changchun 130022, Jilin, People's Republic of China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Agroproducts Processing Technology at Jilin Provincial Universities, Education Department of Jilin Provincial Government, and College of Food Science and Engineering, Changchun University, Satellite Road 6543, Changchun 130022, Jilin, People's Republic of China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, and College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China. Electronic address: drlidan@yahoo.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
mesh_terms: D001105:Archaea / Q000201:enzymology; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D017124:Conserved Sequence; D003505:Cyclodextrins / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013213:Starch / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D003505:Cyclodextrins; D009844:Oligosaccharides; D011134:Polysaccharides; C021705:maltooligosaccharides; C008315:maltodextrin; D013213:Starch; D006026:Glycoside Hydrolases; C082507:amylopullulanase
keywords: Amylodextrin; Caldivirga maquilingensis; Cyclomaltodextrinase; Linear malto-oligosaccharide; Pullulanase