title: On the stability of parainfluenza virus 5 F proteins.
abstract: The crystal structure of the F protein (prefusion form) of the paramyxovirus parainfluenza virus 5 (PIV5) WR isolate was determined. We investigated the basis by which point mutations affect fusion in PIV5 isolates W3A and WR, which differ by two residues in the F ectodomain. The P22 stabilizing site acts through a local conformational change and a hydrophobic pocket interaction, whereas the S443 destabilizing site appears sensitive to both conformational effects and amino acid charge/polarity changes.
authors.affiliation: Department of Molecular Biosciences Northwestern University, Evanston, Illinois, USA.
authors.forename: Taylor A
authors.initials: TA
authors.lastname: Poor
authors.affiliation: Department of Molecular Biosciences Northwestern University, Evanston, Illinois, USA.
authors.forename: Albert S
authors.initials: AS
authors.lastname: Song
authors.affiliation: Department of Molecular Biosciences Northwestern University, Evanston, Illinois, USA Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, USA.
authors.forename: Brett D
authors.initials: BD
authors.lastname: Welch
authors.affiliation: Department of Molecular Biosciences Northwestern University, Evanston, Illinois, USA Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Kors
authors.affiliation: Department of Structural Biology, Stanford University, Stanford, California, USA.
authors.forename: Theodore S
authors.initials: TS
authors.lastname: Jardetzky
authors.affiliation: Department of Molecular Biosciences Northwestern University, Evanston, Illinois, USA Howard Hughes Medical Institute, Northwestern University, Evanston, Illinois, USA ralamb@northwestern.edu.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Lamb
mesh_terms: D020816:Amino Acid Motifs; D018360:Crystallography, X-Ray; D006801:Humans; D009154:Mutation; D045402:Parainfluenza Virus 5 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055550:Protein Stability; D017434:Protein Structure, Tertiary; D019351:Rubulavirus Infections / Q000821:virology; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014760:Viral Fusion Proteins