title: A high-throughput screening assay for identification of inhibitors of the A1AO-ATP synthase of the rumen methanogen Methanobrevibacter ruminantium M1.
abstract: We report the development of a high-throughput screening platform to identify inhibitors of the membrane-bound A1Ao-ATP synthase from the rumen methanogen Methanobrevibacter ruminantium M1. Inhibitors identified in the screen were tested against growing cultures of M. ruminantium, validating the approach to identify new inhibitors of methanogens.
authors.affiliation: Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand.
authors.forename: Htin Lin
authors.initials: HL
authors.lastname: Aung
authors.affiliation: AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: Debjit
authors.initials: D
authors.lastname: Dey
authors.affiliation: AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Janssen
authors.affiliation: AgResearch Limited, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: Ron S
authors.initials: RS
authors.lastname: Ronimus
authors.affiliation: Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand. Electronic address: gregory.cook@otago.ac.nz.
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Cook
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D057166:High-Throughput Screening Assays; D041581:Methanobrevibacter / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D010802:Phylogeny; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012417:Rumen / Q000382:microbiology*
chemical_list: D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases
keywords: A(1)A(O)-ATP synthase; High-throughput screening; Inhibitor; Methane; Methanobrevibacter ruminantium