title: Role of osteopontin in the regulation of human bladder cancer proliferation and migration in T24 cells.
abstract: Osteopontin (OPN), a secreted acid glycoprotein with a variety of functions, promotes tumor proliferation, differentiation, invasion and metastasis. The aim of the current study was to investigate whether OPN may serve as a potential therapeutic target for human bladder cancer. RNA interference (RNAi) was performed to downregulate the expression of the OPN gene in T24 human bladder cancer cells. The mRNA and protein expression levels of OPN following RNAi were determined using reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. In addition, the cell cycle progression, apoptosis and proliferation were investigated using by flow cytometric analysis and MTT assay. The cell invasion ability was measured using a Matrigel transwell assay. The mRNA and protein expression levels of OPN were found to be significantly downregulated following RNAi. The proliferation and invasion of T24 cells were significantly inhibited in vitro. In conclusion, RNAi‑targeting OPN may inhibit the proliferation, invasion and tumorigenicity of human bladder cancer cells. Therefore, OPN may serve as a potential therapeutic target for human bladder cancer.
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Song-Tao
authors.initials: ST
authors.lastname: Xu
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Fan
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Fu-Hua
authors.initials: FH
authors.lastname: Zhang
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Xu
authors.affiliation: Department of Clinical Medicine, Luohe Medical College, Luohe, Henan 462002, P.R. China.
authors.forename: Yong-Chao
authors.initials: YC
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
chemical_list: D034741:RNA, Small Interfering; D053495:Osteopontin