title: A validated HPLC method for the analysis of herbal teas from three chemotypes of Brazilian Lippia alba.
abstract: Infusions and decoctions of three chemotypes of Lippia alba (Mill.) N. E. Brown (Verbenaceae) were investigated for their quantitative profiles by HPLC-DAD-ESI-MS analyses. An RP-HPLC method was developed which permitted the quality control of the preparations. The correct choice of the column allowed the detailed characterization of the constituents in a total analysis time of 35 min. The HPLC method was accordingly validated for linearity range, LOD, LOQ, accuracy and precision. For the quantitative analysis the three major phytochemical groups were taken into consideration, namely iridoids, phenylpropanoids and flavonoids. Comparative quantitative analyses revealed significant differences among the chemotypes that should be taken into account in the uses of the herbal teas. The developed HPLC-UV assay proved to be an efficient and alternative method for the discrimination of the three chemotypes. This is the first report of detailed analysis of the chemical composition of the constituents of L. alba chemotypes' teas.
authors.affiliation: Department of Chemistry, Building of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff, 6, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Timóteo
authors.affiliation: Department of Chemistry, Building of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff, 6, 50019 Sesto Fiorentino (FI), Italy. Electronic address: anastasiakarioti@yahoo.it.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Karioti
authors.affiliation: Faculty of Pharmacy, Federal University of Rio de Janeiro, Bloco A, 2° andar, Ilha do Fundão, 21941-590 Rio de Janeiro, Brazil.
authors.forename: Suzana G
authors.initials: SG
authors.lastname: Leitão
authors.affiliation: Department of Chemistry, Building of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff, 6, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Franco Francesco
authors.initials: FF
authors.lastname: Vincieri
authors.affiliation: Department of Chemistry, Building of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff, 6, 50019 Sesto Fiorentino (FI), Italy.
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Bilia
mesh_terms: D001628:Beverages / Q000032:analysis*; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis; D032411:Lippia / Q000737:chemistry*
chemical_list: D005419:Flavonoids
keywords: Chemotypes; Flavonoid glycosides; HPLC–DAD–ESI-MS; Herbal teas; Iridoids; Lippia alba; Phenylpropanoid glycosides