title: β-Spectrin regulates the hippo signaling pathway and modulates the basal actin network.
abstract: Emerging evidence suggests functional regulation of the Hippo pathway by the actin cytoskeleton, although the detailed molecular mechanism remains incomplete. In a genetic screen, we identified a requirement for β-Spectrin in the posterior follicle cells for the oocyte repolarization process during Drosophila mid-oogenesis. β-spectrin mutations lead to loss of Hippo signaling activity in the follicle cells. A similar reduction of Hippo signaling activity was observed after β-Spectrin knockdown in mammalian cells. We further demonstrated that β-spectrin mutations disrupt the basal actin network in follicle cells. The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the β-spectrin mutations and the loss of the Hippo signaling activity phenotype.
authors.affiliation: From the Division of Life Science and Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China and.
authors.forename: Kenneth Kin Lam
authors.initials: KK
authors.lastname: Wong
authors.affiliation: the Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544.
authors.forename: Wenyang
authors.initials: W
authors.lastname: Li
authors.affiliation: From the Division of Life Science and Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China and.
authors.forename: Yanru
authors.initials: Y
authors.lastname: An
authors.affiliation: From the Division of Life Science and.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: From the Division of Life Science and.
authors.forename: Zhuoheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: the Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544.
authors.forename: Yibin
authors.initials: Y
authors.lastname: Kang
authors.affiliation: From the Division of Life Science and Center of Systems Biology and Human Health, School of Science and Institute for Advanced Study, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China and yany@ust.hk.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D003599:Cytoskeleton / Q000235:genetics / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009865:Oocytes / Q000254:growth & development / Q000378:metabolism; D009866:Oogenesis / Q000235:genetics*; D006080:Ovarian Follicle / Q000254:growth & development / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D013049:Spectrin / Q000235:genetics*; D000099317:Hippo Kinases
chemical_list: D000199:Actins; D029721:Drosophila Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017346:Protein Serine-Threonine Kinases; D013049:Spectrin; C499474:hpo protein, Drosophila; D000099317:Hippo Kinases
keywords: Cell Signaling; Drosophila; Epithelial Cell; Hippo Pathway; Spectrin