title: Aberrant EphB/ephrin-B expression in experimental gastric lesions and tumor cells.
abstract: OBJECTIVE
  To determine whether the expression profiles of EphB receptor and ephrin-B ligand can be used as markers for dysplastic/oncogenic transformation in gastric mucosa.
  
  
  METHODS
  The protein expression and localization of EphB and ephrin-B in normal, ulcerated regenerating, and dysplastic gastric mucosa were examined in a rat experimental model by immunolabeling, and mRNA expression was assessed in four human gastric carcinoma cell lines by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  Ephrin-B- and EphB-expressing regions were divided along the pit-gland axis in normal gastric units. EphB2 was transiently upregulated in the experimental ulcer, and its expression domain extended to gastric pits and/or the luminal surface where ephrin-B-expressing pit cells reside. EphB2, B3, and B4 and ephrin-B1 were coexpressed in the experimental gastric dysplasia, and more than one ligand-receptor pair was highly expressed in each of the gastric carcinoma cell lines.
  
  
  CONCLUSIONS
  Robust and stable coexpression of EphB and ephrin-B is a feature common to experimentally induced gastric dysplasia and human gastric carcinoma cell lines as compared to normal gastric and ulcerated regenerating epithelia. Thus, EphB/ephrin-B may be a useful marker combination for dysplastic/oncogenic transformation in gastric cancer.
authors.affiliation: Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa, Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Uchiyama
authors.affiliation: Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa, Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan.
authors.forename: Noritaka
authors.initials: N
authors.lastname: Saeki
authors.affiliation: Shintaro Uchiyama, Noritaka Saeki, Kazushige Ogawa, Department of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 598-8531, Japan.
authors.forename: Kazushige
authors.initials: K
authors.lastname: Ogawa
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004195:Disease Models, Animal; D036342:Ephrins / Q000235:genetics / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008024:Ligands; D008297:Male; D011230:Precancerous Conditions / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D011916:Rats, Inbred F344; D036081:Receptors, Eph Family / Q000235:genetics / Q000378:metabolism*; D012038:Regeneration; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013276:Stomach Ulcer / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D036342:Ephrins; D008024:Ligands; D012333:RNA, Messenger; D036081:Receptors, Eph Family
keywords: Coexpression; EphB; Ephrin-B; Gastric carcinoma cell line; Gastric dysplasia; Gastric ulcer