title: LGR5 expression in oral epithelial dysplasia and oral squamous cell carcinoma.
abstract: OBJECTIVE
  LGR5 is pivotal to oral cavity development and is implicated in epithelial malignancy whereby stimulation of LGR5 potentiates canonical Wnt signaling. This investigation tested our hypothesis of a correlation between LGR5 expression and the severity of oral epithelial dysplasia (OED) and oral squamous cell carcinoma (OSCC).
  
  
  METHODS
  Immunoreactive LGR5 protein expression was quantified in 342 tissue samples ranging in disease severity from normal through mild and moderate or severe OED to OSCC.
  
  
  RESULTS
  LGR5 was restricted to the basal layers for normal tissues, projected to the stratum granulosum in severe dysplasia, intense in carcinoma nests of well-differentiated OSCC, but uniformly diffuse throughout poorly differentiated OSCC. Median LGR5 immunoreactivity index scores increased with disease severity: mild dysplasia = 1 < moderate or severe dysplasia = 2.5 < OSCC = 6.
  
  
  CONCLUSIONS
  Inclusion of LGR5 in a panel of immunohistochemical biomarkers may improve identification of increased potential for malignancy in OED.
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Dalley
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia.
authors.forename: Ahmad A
authors.initials: AA
authors.lastname: Abdul Majeed
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia; The University of Queensland, School of Dentistry, Brisbane, Queensland, Australia.
authors.forename: Luke P
authors.initials: LP
authors.lastname: Pitty
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia; The University of Queensland, School of Dentistry, Brisbane, Queensland, Australia.
authors.forename: Aidan G
authors.initials: AG
authors.lastname: Major
authors.affiliation: The University of Queensland, UQ Centre for Clinical Research, Brisbane, Queensland, Australia; The University of Queensland, School of Dentistry, Brisbane, Queensland, Australia; The Australian Centre for Oral Oncology Research & Education, Brisbane, Queensland, Australia. Electronic address: camile@oralmedpath.com.au.
authors.forename: Camile S
authors.initials: CS
authors.lastname: Farah
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D012189:Retrospective Studies; D012720:Severity of Illness Index
chemical_list: D014408:Biomarkers, Tumor; C116884:LGR5 protein, human; D043562:Receptors, G-Protein-Coupled