title: Fluorescent labelling of DNA on superparamagnetic nanoparticles by a perylene bisimide derivative for cell imaging.
abstract: N,N'-Bis[tris-(2-aminoethyl) amine]-3,4,9,10-perylenetetracarboxylic diimide (PBI-TRIS), nonfluorescent dye was used to fluorescent labelling of DNA. For this aim, (3-aminopropyl) triethoxysilane (APTS) modified superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized to provide a suitable surface for binding of DNA. Amine functionalized nanoparticles showed a high immobilization capacity (82.70%) at 25mg of nanoparticle concentration for Calf thymus DNA. Binding capacity of PBI-TRIS to DNA-SPION was also found as 1.93μM on 25mg of nanoparticles by using UV-vis spectroscopy. Binding of PBI-TRIS to DNA onto nanoparticles was also characterized by scanning electron microscopy and infrared spectroscopy. The confocal images of PBI-TRIS labelled DNA-SPION and breast cells were taken at 488 and 561.7nm of excitation wavelengths. Cell image was also compared with a commercial dye, DAPI at 403.7nm of excitation wavelength. Results showed that PBI-TRIS can be used for cell staining.
authors.affiliation: Selcuk University, Faculty of Science, Department of Chemistry, 42075 Konya, Turkey. Electronic address: maltasesra@gmail.com.
authors.forename: Esra
authors.initials: E
authors.lastname: Maltas
authors.affiliation: Selcuk University, Faculty of Science, Department of Chemistry, 42075 Konya, Turkey.
authors.forename: Sait
authors.initials: S
authors.lastname: Malkondu
authors.affiliation: Selcuk University, Faculty of Science, Department of Biology, 42075 Konya, Turkey; Selcuk University, Advanced Technology Research and Application Center, Konya, Turkey.
authors.forename: Pembegul
authors.initials: P
authors.lastname: Uyar
authors.affiliation: Selcuk University, Faculty of Science, Department of Chemistry, 42075 Konya, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozmen
mesh_terms: D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D005029:Ethylenediamines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007094:Imides / Q000737:chemistry*; D058185:Magnetite Nanoparticles / Q000737:chemistry; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D057054:Molecular Imaging / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010569:Perylene / Q000031:analogs & derivatives* / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005029:Ethylenediamines; D005456:Fluorescent Dyes; D007094:Imides; D058185:Magnetite Nanoparticles; C000601350:N,N'-bis(tris-(2-aminoethyl)amine)-3,4,9,10-perylenetetracarboxylic diimide; C510697:perylene bisimide; D010569:Perylene; D004247:DNA; C427203:calf thymus DNA
keywords: Calf thymus DNA; Cell image; Fluorescent labelling; Perylene bisimide