title: Surface biocompatibility of differently textured titanium implants with mesenchymal stem cells.
abstract: OBJECTIVE
  The major challenge for contemporary dentistry is restoration of missing teeth; currently, dental implantation is the treatment of choice in this circumstance. In the present study, we assessed the interaction between implants and Dental Pulp Stem Cells (DPSCs) in vitro by means of 3D cell culture in order to better simulate physiological conditions.
  
  
  METHODS
  Sorted CD34+ DPSCs were seeded onto dental implants having either a rough surface (TriVent) or one coated with a ceramic layer mimicking native bone (TiUnite). We evaluated preservation of DPSC viability during osteogenic differentiation by an MTT assay and compared mineralized matrix deposition with SEM analysis and histological staining; temporal expression of osteogenic markers was evaluated by RT-PCR and ELISA.
  
  
  RESULTS
  Both surfaces are equally biocompatible, preserve DPSC viability, stimulate osteogenic differentiation, and increase the production of VEGF. A slight difference was observed between the two surfaces concerning the speed of DPSC differentiation.
  
  
  CONCLUSIONS
  Our study of the two implant surfaces suggests that TriVent, with its roughness, is capable of promoting cell differentiation a bit earlier than the TiUnite surface, although the latter promotes greater cell proliferation.
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie ed Istologia Medica, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Pasqualina
authors.initials: P
authors.lastname: Naddeo
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Università degli Studi di Foggia, Foggia, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Laino
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie ed Istologia Medica, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: La Noce
authors.affiliation: Dipartimento di Dipartimento di Scienze Mediche, Orali e Biotecnologiche, Università degli Studi "G. D'Annunzio" Chieti-Pescara, Chieti, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Piattelli
authors.affiliation: Dipartimento di Odontostomatologia e Discipline Chirurgiche, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: De Rosa
authors.affiliation: Dipartimento di Dipartimento di Scienze Mediche, Orali e Biotecnologiche, Università degli Studi "G. D'Annunzio" Chieti-Pescara, Chieti, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Iezzi
authors.affiliation: Dipartimento di Odontostomatologia e Discipline Chirurgiche, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Laino
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie ed Istologia Medica, Seconda Università degli Studi di Napoli, Napoli, Italy. Electronic address: francesca.paino@unina2.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Paino
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie ed Istologia Medica, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Papaccio
authors.affiliation: Dipartimento di Medicina Sperimentale, Sezione di Biotecnologie ed Istologia Medica, Seconda Università degli Studi di Napoli, Napoli, Italy.
authors.forename: Virginia
authors.initials: V
authors.lastname: Tirino
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D015921:Dental Implants; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology; D016133:Polymerase Chain Reaction; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D015921:Dental Implants; D014025:Titanium
keywords: Biocompatibility; Cell proliferation; DPSCs; Osteodifferentiation; Surface texture; Titanium implants