title: Potent anti-prostate cancer immune response induced by dendritic cells transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells.
abstract: OBJECTIVE
  To test the effect of dendritic cells (DCs) transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells (CIKs) against prostate cancer.
  
  
  METHODS
  Flow cytometry was used to detect the surface markers of the co-cultured cells, and cytotoxicity against prostate cancer cells in vitro as well as antitumor activities in vivo were observed.
  
  
  RESULTS
  Our results showed that Ad-4-1BBL-transduced DCs could increase percentage of CD3(+)CD56(+) cells in CIKs, and CIKs co-cultured with Ad-4-1BBL-transduced DCs could augment the secretion of IL-12 and IFN-γ and decrease TGF-β production. In addition, Ad-4-1BBL-transduced DCs enhanced the cytotoxicity of CIKs against prostate cancer and resulted in inhibition of tumor growth and tumor-bearing animals' survival.
  
  
  CONCLUSIONS
  These results demonstrate that 4-1BBL-engineered DCs can improve CIKs cytotoxicity against prostate cancer cells.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Kuang
authors.initials: K
authors.lastname: Youlin
authors.forename: Kang
authors.initials: K
authors.lastname: Jian
authors.forename: Liang
authors.initials: L
authors.lastname: Siming
authors.forename: Zhang
authors.initials: Z
authors.lastname: Li
authors.forename: He
authors.initials: H
authors.lastname: Weiyang
authors.forename: Liu
authors.initials: L
authors.lastname: Chaodong
authors.forename: Gou
authors.initials: G
authors.lastname: Xin
mesh_terms: D053330:4-1BB Ligand / Q000235:genetics / Q000276:immunology; D000256:Adenoviridae; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy; D014161:Transduction, Genetic
chemical_list: D053330:4-1BB Ligand; D016207:Cytokines
keywords: 4-1BBL; cytokine-induced killer cells; dendritic cells; immunotherapy