title: [The effects of intermittent hypobaric hypoxia on myocardial ischemia/reperfusion injury and ZFP580 expression].
abstract: OBJECTIVE
  To elucidate whether ZFP580 is involved in the cardioprotective effects of intermittent hypobaric hypoxia (IHH) against myocardial ischemia/reperfusion (I/R) injury.
  
  
  METHODS
  Thirty two male Wistar rats were randomly divided into 2 groups (n = 16): normoxia control group and IHH preconditioning group. Rats in IHH group were exposed in a hypobaric chamber (equivalent to an altitude of 5 000 m) for a 6 h period each day for 42 d. Plasma was collected and lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) were measured after 2 h of myocardial I/R injury. ZFP580 protein expression in myocardial tissue was assayed by Western blot. Other 8 rats in each group were used to evaluate I/R-induced cardiac infarction by TTC staining. Lentivirus-mediated gene transfection was performed in H9c2 cells 72 h prior to simulated ischemia/reperfusion (SI/R) exposure. The degree of cell apoptosis was determined by annexin V/7-AAD staining and flow cytometry analysis.
  
  
  RESULTS
  Compared with normoxia control group, adaptation to IHH attenuated infarct size and plasma leakage of LDH and CK-MB. In addition, ZFP580 expression in the myocardium was up-regulated by IHH. The results of gene transfection showed that ZFP580 overexpression significantly inhibited cells apoptosis induced by SI/R.
  
  
  CONCLUSIONS
  Our findings demonstrate that the cardioprotective effect of IHH against I/R injury is mediated via ZFP580, a novel transcription factor, with anti-apoptotic roles in myocardial cells.
authors.affiliation: Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force, Tianjin, China.
authors.forename: Xiang-yan
authors.initials: XY
authors.lastname: Meng
authors.forename: Hai-long
authors.initials: HL
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Guo
authors.forename: Wen-cheng
authors.initials: WC
authors.lastname: Zhang
authors.forename: Rui-cheng
authors.initials: RC
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D002460:Cell Line; D052279:Creatine Kinase, MB Form / Q000378:metabolism; D000860:Hypoxia / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology*; D009206:Myocardium / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors; C000598274:ZFP580 protein, rat; D007770:L-Lactate Dehydrogenase; D052279:Creatine Kinase, MB Form