title: Bacterial Efficacy of Ca(oH)2 Against E.faecalis Compared with three Dental Lasers on Root Canal Dentin- An Invitro Study.
abstract: OBJECTIVE
  The aim of this study was to evaluate bactericidal effect of Ca(OH)2 compared with 810 nm diode, 980 nm diode, and Nd:YAG lasers on root canal dentin against E.faecalis.
  
  
  METHODS
  Sixty five freshly extracted human mandibular single rooted teeth were selected for the study. The apical third of these roots was gradually enlarged until reaching the ISO 40- K file. The samples were divided into 4 groups, each containing 15 teeth and 5 teeth for control group. Group-1: 810 nm Diode; Group-2: 980 nm Diode; Group-3: Nd:YAG; Group-4: Ca(OH)2. 50μL of the E.faecalis ATCC 29212 strand was incubated in 1 mL of Brain Heart Infusion Broth (BHI) culture medium in37(o)C incubator for 4h. The concentration of the inoculation was then adjusted for a degree of turbidity which was adjusted to 0.5 McFarland scale. Later from the incubated broth, 10μL of E.faecalis culture was inoculated into the main canal and were sealed.
  
  
  RESULTS
  The incubated plates were checked for growth and the colony was counted using colony counter and the results are interpreted. There was statistically significant difference (p<0.05) amongst the Ca(OH)2 group regarding the laser groups.
  
  
  CONCLUSIONS
  The teeth irradiated with the Nd:YAG laser had significantly higher bacterial reduction than all the other groups and the respective control groups.
authors.affiliation: Reader, Department of Conservative Dentistry and Endodontics, Sibar Dental College , Guntur, Andhra Pradesh, India .
authors.forename: Narasimha Reddy
authors.initials: NR
authors.lastname: Kanumuru
authors.affiliation: Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Dental College , Guntur, Andhra Pradesh, India .
authors.forename: Rama
authors.initials: R
authors.lastname: Subbaiah
keywords: Ca(OH)2; Diode laser; E.Faecalis; Nd:YAG laser