title: Dehydrozingerone exerts beneficial metabolic effects in high-fat diet-induced obese mice via AMPK activation in skeletal muscle.
abstract: Dehydrozingerone (DHZ) exerts beneficial effects on human health; however, its mechanism of action remains unclear. Here, we found that DHZ suppressed high-fat diet-induced weight gain, lipid accumulation and hyperglycaemia in C57BL/6 mice and increased AMP-activated protein kinase (AMPK) phosphorylation and stimulated glucose uptake in C2C12 skeletal muscle cells. DHZ activated p38 mitogen-activated protein kinase (MAPK) signalling in an AMPK-dependent manner. Inhibiting AMPK or p38 MAPK blocked DHZ-induced glucose uptake. DHZ increased GLUT4 (major transporter for glucose uptake) expression in skeletal muscle. Glucose clearance and insulin-induced glucose uptake increased in DHZ-fed animals, suggesting that DHZ increases systemic insulin sensitivity in vivo. Thus, the beneficial health effects of DHZ could possibly be explained by its ability to activate the AMPK pathway in skeletal muscle.
authors.affiliation: Department of Anatomy, Korea University College of Medicine, Seoul, South Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hong Min
authors.initials: HM
authors.lastname: Kim
authors.forename: Eun Soo
authors.initials: ES
authors.lastname: Lee
authors.forename: Nami
authors.initials: N
authors.lastname: Kim
authors.forename: Jung Ok
authors.initials: JO
authors.lastname: Lee
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Na Yeon
authors.initials: NY
authors.lastname: Park
authors.forename: Joo Yeon
authors.initials: JY
authors.lastname: Jo
authors.forename: Bo Young
authors.initials: BY
authors.lastname: Ham
authors.forename: Si Hyun
authors.initials: SH
authors.lastname: Han
authors.forename: Sun Hwa
authors.initials: SH
authors.lastname: Park
authors.forename: Choon Hee
authors.initials: CH
authors.lastname: Chung
authors.forename: Hyeon Soo
authors.initials: HS
authors.lastname: Kim
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects / Q000235:genetics; D001786:Blood Glucose / Q000378:metabolism; D002478:Cells, Cultured; D003474:Curcumin / Q000031:analogs & derivatives; D003847:Deoxyglucose / Q000378:metabolism; D059305:Diet, High-Fat; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000096:biosynthesis; D006943:Hyperglycemia / Q000188:drug therapy; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000378:metabolism*; D009765:Obesity; D010766:Phosphorylation / Q000187:drug effects; D034622:RNA Interference; D034741:RNA, Small Interfering; D051381:Rats; D013343:Styrenes / Q000494:pharmacology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D034741:RNA, Small Interfering; C495693:Slc2a4 protein, mouse; D013343:Styrenes; C052933:methyl-3-methoxy-4-hydroxystyryl ketone; D003847:Deoxyglucose; D048051:p38 Mitogen-Activated Protein Kinases; D055372:AMP-Activated Protein Kinases; D003474:Curcumin; D005947:Glucose
keywords: AMPK; curcumin analogue; dehydrozingerone; glucose uptake; metabolism