title: Determination of alanine aminotransferase with an electrochemical nano ir-C biosensor for the screening of liver diseases.
abstract: Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H2O2 concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0-544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.
authors.affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxh300@case.edu.
authors.forename: Chang-Jung
authors.initials: CJ
authors.lastname: Hsueh
authors.affiliation: Department of Biology, Brown University, 69 Brown Street, Providence, RI 02912, USA. joanne_wang@brown.edu.
authors.forename: Joanne H
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. liming.dai@case.edu.
authors.forename: Liming
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA. cxl9@case.edu.
authors.forename: Chung-Chiun
authors.initials: CC
authors.lastname: Liu