title: [The role of Treg/Thl7 and calprotectin in ulcerative colitis rat model].
abstract: OBJECTIVE
  To investigate the roles of helper T cells (Th), regulatory T cells (Treg) and calprotectin in the pathogenesis of ulcerative colitis (UC) in the rat model.
  
  
  METHODS
  Thirty rats were randomly divided into normal control (NC) group and UC model (UC) group. The rats of UC group were replicated using the mixed solution of 2, 4,6 trinitrobenzene sulfonic acid (TNBS) / ethanol through the intestinal tract. The rats of NC group were given NaCI solution enema. Three weeks after UC modeling, the morphology changes of colon tissue were observed. The serum levels interleukin (IL)-1β, IL-10, IL-17, IL-23 and calprotectin (CP) were detected by enzyme-linked immunosorbent assay. The changes of Treg in peripheral blood were measured by flow cytometry. The expressions of IL-17, CP, and transcriptional regulatory proteins of decision Treg cell differentiation and function (FoxP3) in colon tissue were detected by Western blot and immunohistochemistry.
  
  
  RESULTS
  UC group was observed with obvious colon tissue injury and inflammatory cells infiltration in intestinal tissue, compared with NC group, the expression of IL-1β,IL-17,IL-23,CP in serum were increased, while the expression of IL-10, the number of Treg were decreased in UC group (P<0. 05 or P(<0. 01). Compared with NC group, the expression of IL-17, CP in colon tissue were significantly increased in UC group (P(<0.05), and the expression of FoxP3 was increased (P(0. 05).
  
  
  CONCLUSIONS
  Expressions of calprotectin, IL-17 of intestinal tissue, serum are increased in rat of ulcerative colitis, which to lead to the imbalance of Treg/Thl7 cells, and elevate inflammatory responses. These factors have promote the occurrence and development of ulcerative colitis.
authors.affiliation: Department of Internal Medicine, Fourth Affiliated  Hospital, Anhui Medical University, Hefei, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.forename: Xiu-min
authors.initials: XM
authors.lastname: Xuan
authors.forename: Ling
authors.initials: L
authors.lastname: Tong
authors.forename: Qi
authors.initials: Q
authors.lastname: Huang
authors.forename: Li-min
authors.initials: LM
authors.lastname: Zhu
authors.forename: Hai-ling
authors.initials: HL
authors.lastname: Ruan
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000276:immunology* / Q000473:pathology; D016753:Interleukin-10 / Q000097:blood; D020381:Interleukin-17 / Q000097:blood; D053583:Interleukin-1beta / Q000097:blood; D053759:Interleukin-23 / Q000097:blood; D007422:Intestines / Q000473:pathology; D039841:Leukocyte L1 Antigen Complex / Q000097:blood*; D051381:Rats; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D053583:Interleukin-1beta; D053759:Interleukin-23; D039841:Leukocyte L1 Antigen Complex; D016753:Interleukin-10