title: Quantifying the vascular response to ischemia with speckle variance optical coherence tomography.
abstract: Longitudinal monitoring techniques for preclinical models of vascular remodeling are critical to the development of new therapies for pathological conditions such as ischemia and cancer. In models of skeletal muscle ischemia in particular, there is a lack of quantitative, non-invasive and long term assessment of vessel morphology. Here, we have applied speckle variance optical coherence tomography (OCT) methods to quantitatively assess vascular remodeling and growth in a mouse model of peripheral arterial disease. This approach was validated on two different mouse strains known to have disparate rates and abilities of recovering following induction of hind limb ischemia. These results establish the potential for speckle variance OCT as a tool for quantitative, preclinical screening of pro- and anti-angiogenic therapies.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B #351631, Nashville, TN 37235, USA.
authors.forename: Kristin M
authors.initials: KM
authors.lastname: Poole
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B #351631, Nashville, TN 37235, USA.
authors.forename: Devin R
authors.initials: DR
authors.lastname: McCormack
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B #351631, Nashville, TN 37235, USA.
authors.forename: Chetan A
authors.initials: CA
authors.lastname: Patil
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B #351631, Nashville, TN 37235, USA.
authors.forename: Craig L
authors.initials: CL
authors.lastname: Duvall
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, VU Station B #351631, Nashville, TN 37235, USA.
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Skala
keywords: (100.2960) Image analysis; (110.6150) Speckle imaging; (170.2655) Functional monitoring and imaging; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography