title: [Study on seeds after-ripening regulation and key techniques for seedling of Pulsatilla cernua].
abstract: OBJECTIVE
  The seeds of Pulsatilla cernua were used as tested materials for screening and establishing the main factors with different levels to control fast development of seed embryonic and seedlings of Pulsatilla cernua.
  
  
  METHODS
  The main factors with different levels for development of seed embryonic and seedlings of Pulsatilla cernua were investigated through repeated experiments with multifactorial and cross.
  
  
  RESULTS
  The method for development of seedlings and seeds germination of Pulsatilla cernua were to soak the seeds in the mixed solutions with 2.40 mg/L KT, 2.80 mg/L GA3 and 0.30 -0.70 mg/L 2,4-D for 24 h. The seeds and sand (1:2) were mixed, treated with temperature change in 63 - 70 d. The extent of temperature change and time were (23 ± 2) degrees C and 14 h in day, while (10 ± 2) degrees C and 10 h in night. The incidence rate of the embryo with cotyledons was 95.1%, and the germination rate of seed was 92.3%.
  
  
  CONCLUSIONS
  The plant regeneration control technology for development of seed embryonic and seedling of Pulsatilla cernua have been solved, which is suitable for industrial seedlings of Pulsatilla cernua.
authors.affiliation: Tonghua Normal University, China. gudizhou@163.com
authors.forename: Di-Zhou
authors.initials: DZ
authors.lastname: Gu
authors.forename: Li-Fan
authors.initials: LF
authors.lastname: Zhang
authors.forename: Qiu-Shuang
authors.initials: QS
authors.lastname: Wang
authors.forename: Xue-Shi
authors.initials: XS
authors.lastname: Zhang
mesh_terms: D018525:Germination; D031946:Pulsatilla / Q000254:growth & development*; D036226:Seedlings; D012639:Seeds / Q000254:growth & development*