title: Preliminary study on pathogenesis of bronchial asthma in children.
abstract: BACKGROUND
  The etiology and pathogenesis of bronchial asthma remain unclear. This study is to investigate the risk factors related to bronchial asthma onset in children from genetics and immunology and preliminarily reveal the pathogenesis of bronchial asthma in children.
  
  
  METHODS
  Real-time quantitative PCR was adopted to detect the expression level of TRPV1 gene and mRNA and enzyme-linked immunosorbent assay method to the total immunoglobulin E level and levels of IL-4, IL-5, and IFN-γ in serum in peripheral venous blood for children in two groups. Logistic regression analysis was applied to analyze the most essential factors inducing bronchial asthma in children.
  
  
  RESULTS
  The mRNA level of TRPV1 in peripheral blood in the case group was higher than that in the control group (P < 0.01). The levels of IL-4, IL-5, and eosinophils in serum in the case group were markedly higher than those in the control group (P < 0.01), while IFN-γ level in the case group was lower than that in the control group (P < 0.01). The results of logistic regression analysis indicated that TRPV1 expression level, IL-4 level, and rs4790522 site mutation were the main risk factors inducing bronchial asthma in children.
  
  
  CONCLUSIONS
  The levels of TRPV1 gene expression and Th1/Th2 cytokines have a close relationship with asthma onset in children, which provides theoretical evidences for molecular targeted treatment in children with bronchial asthma.
authors.affiliation: 1] Rehabilitation Centre, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China [2] Department of Chinese Internal Medicine, the First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan, China.
authors.forename: Ya-Feng
authors.initials: YF
authors.lastname: Ren
authors.affiliation: Department of Pediatrics, People's Hospital of Zhengzhou, Zhengzhou, Henan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pediatrics, People's Hospital of Zhengzhou, Zhengzhou, Henan, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Xing
authors.affiliation: Department of Pediatrics, People's Hospital of Zhengzhou, Zhengzhou, Henan, China.
authors.forename: Hai-Shan
authors.initials: HS
authors.lastname: Guan
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Bo-Ai
authors.initials: BA
authors.lastname: Zhang
authors.affiliation: Henan Provincial People's Hospital, Zhengzhou, Henan, China.
authors.forename: Chuan-Liang
authors.initials: CL
authors.lastname: Chen
authors.affiliation: School of Electrical Engineering, Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D001249:Asthma / Q000235:genetics* / Q000276:immunology* / Q000503:physiopathology*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012044:Regression Analysis; D012307:Risk Factors; D050916:TRPV Cation Channels / Q000235:genetics / Q000378:metabolism*
chemical_list: C573415:IFNG protein, human; C508598:IL4 protein, human; C508599:IL5 protein, human; D015848:Interleukin-5; D050916:TRPV Cation Channels; C494482:TRPV1 protein, human; D015847:Interleukin-4; D007371:Interferon-gamma