title: Effect of surface chemistry on the integrin induced pathway in regulating vascular endothelial cells migration.
abstract: The migration of vascular endothelial cells (ECs) is essential for reendothelialization after implantation of cardiovascular biomaterials. Reendothelialization is largely determined by surface properties of implants. In this study, surfaces modified with various chemical functional groups (CH3, NH2, COOH, OH) prepared by self-assembled monolayers (SAMs) were used as model system. Expressions and distributions of critical proteins in the integrin-induced signaling pathway were examined to explore the mechanisms of surface chemistry regulating EC migration. The results showed that SAMs modulated cell migration were in the order CH3>NH2>OH>COOH, determined by differences in the expressions of focal adhesion components and Rho GTPases. Multiple integrin subunits showed difference in a surface chemistry-dependent manner, which induced a stepwise activation of signaling cascades associated with EC migration. This work provides a broad overview of surface chemistry regulated endothelial cell migration and establishes association among the surface chemistry, cell migration behavior and associated integrin signaling events. Understanding the relationship between these factors will help us to understand the surface/interface behavior between biomaterials and cells, reveal molecular mechanism of cells sensing surface characterization, and guide surface modification of cardiovascular implanted materials.
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
authors.forename: Hongchi
authors.initials: H
authors.lastname: Yu
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, Department of Material Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Fu-zhai
authors.initials: FZ
authors.lastname: Cui
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, PR China.
authors.forename: Hans
authors.initials: H
authors.lastname: Gregersen
authors.affiliation: Center for Surface Science and Plasma Technology, Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400030, PR China.
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China. Electronic address: liuxiaohg@scu.edu.cn.
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Liu
mesh_terms: D002465:Cell Movement; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism*; D006801:Humans; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D016023:Integrins
keywords: Cell adhesion/migration; FAK; Focal adhesion; Rho GTPases; Surface chemistry