title: Role of heat shock protein 27 in regulation of glutathione system and apoptosis of Jurkat tumor cells and blood lymphocytes.
abstract: Reaction of the glutathione system of Jurkat tumor cells and blood lymphocytes was evaluated under conditions of culturing with 5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl) isoxazole (KRIBB3), a selective inhibitor of heat shock protein Hsp27. The results indicated the regulatory role of Hsp27 in the maintenance of the functional activities of glutathione reductase, glutathione peroxidase, and realization of apoptotic death of Jurkat cells and blood lymphocytes. Inhibition of Hsp27 in Jurkat tumor cells led to imbalance of the glutathione system and increase of the share of annexin-positive cells.
authors.affiliation: Siberian State Medical University, the Ministry of Health of the Russian Federation, Tomsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ryazantseva
authors.forename: E A
authors.initials: EA
authors.lastname: Stepovaya
authors.forename: O L
authors.initials: OL
authors.lastname: Nosareva
authors.forename: E V
authors.initials: EV
authors.lastname: Konovalova
authors.forename: D S
authors.initials: DS
authors.lastname: Orlov
authors.forename: A I
authors.initials: AI
authors.lastname: Naumova
authors.forename: S A
authors.initials: SA
authors.lastname: Didenko
authors.forename: O N
authors.initials: ON
authors.lastname: Vesnina
authors.forename: E V
authors.initials: EV
authors.lastname: Shakhristova
authors.forename: A P
authors.initials: AP
authors.lastname: Zima
authors.forename: V V
authors.initials: VV
authors.lastname: Novitskii
mesh_terms: D000328:Adult; D000818:Animals; D000840:Anisoles / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005260:Female; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D055551:HSP27 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D055815:Young Adult
chemical_list: C507623:5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)isoxazole; D000840:Anisoles; D055551:HSP27 Heat-Shock Proteins; D007555:Isoxazoles; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005978:Glutathione