title: [Experimental study and mechanism research of 701 diedazhentonggao on acute and chronic soft tissue injury in rabbit].
abstract: OBJECTIVE
  To study the effect and mechanism of 701 Diedazhentonggao on acute and chronic soft tissue injury in rabbit.
  
  
  METHODS
  The rabbit model of acute and chronic soft tissue injury were established by heavy hammer blow method. The tissue damage scored, pain threshold value and blood rheology were detected, and histopathological and inflammatory cytokines in damaged tissue and gene chip were observed.
  
  
  RESULTS
  701 Diedazhentonggao significantly improved the acute soft tissue injury symptoms, increased the pain threshold, reduced the score values of local damage, improved subcutaneous ecchymosis symptoms, reduced the content of inflammatory cytokines in damaged tissue, repaired the injuried tissue, and reduced blood viscosity and the content of IL-1 and 5-HT. But the contents of IL-6 and PGE2 in acute soft tissue injury were not reduced. The gene chip study expressed the drug interfered the pathway of IL-1R and white blood cell cling. 701 Diedazhentonggao also improved the chronic soft tissue injury symptoms and the main performance on anti-inflammatory effect, and reduced the contents of IL-6, IL-1 and 5-HT, but it had little effet on reducing the contents of PGE2, Fbg, blood and histopathological examination.
  
  
  CONCLUSIONS
  701 Diedazhentonggao has ameliorative effect on acute and chronic soft tissue injury, but the effect on acute soft tissue injuries is better than on chronic soft tissue injuries, and its mechanism may be related to reducing the contents of IL-1, IL-6, 5-HT and other related inflammatory mediators.
authors.affiliation: Guangzhou General Pharmaceutical Research Industry, Guangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Deng
authors.forename: Qiu-Ping
authors.initials: QP
authors.lastname: Guo
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Wang
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Guo
authors.forename: Bai-Quan
authors.initials: BQ
authors.lastname: Xiao
mesh_terms: D000818:Animals; D007375:Interleukin-1; D015850:Interleukin-6; D008297:Male; D011817:Rabbits; D015398:Signal Transduction; D017695:Soft Tissue Injuries / Q000188:drug therapy*
chemical_list: D007375:Interleukin-1; D015850:Interleukin-6