title: Mulberry extracts alleviate aβ 25-35-induced injury and change the gene expression profile in PC12 cells.
abstract: Mulberry, which contained high amounts of anthocyanins, has been used in traditional Chinese medicine. Mulberry fruit extracts (ME) have demonstrated the antioxidant activity and neuroprotection. The study was to investigate the neuroprotective efficacy of ME against β-amyloid 25-35- (Aβ 25-35-) induced PC12 cells injury. Cells preincubated with or without ME (200 μg/mL) for 24 h were treated with Aβ 25-35 (20 μmol/L) for another 24 h. Cell viability was assessed by MTT, gene expression profiles were examined by cDNA microarrays, and RT-PCR were used to confirm the results of microarray assays. ME pretreatment was found to neutralize the cytotoxicity and prevent Aβ 25-35-induced cells injury. Analyses of gene expression profile revealed that genes involving cell adhesion, peptidase activity, cytokine activity, ion binding activity, and angiogenesis regulation were significantly modulated by ME pretreatment. Among those genes, Apaf1, Bace2, and Plcb4 were enriched in the "Alzheimer's disease-reference pathway" and downregulated after ME intervention. RT-PCR results showed that ME preincubation could significantly inhibit Aβ 25-35 increased mRNA levels of these three genes. Overall, ME pretreatment could substantially alleviate PC12 cells injury and downregulate expression of AD-related genes, such as Apaf1, Bace2, and Plcb4. This study has a great nutrigenomics interest and brings new and important light in the field of AD intervention.
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China ; Comparative Medicine Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Song
authors.affiliation: Tianjin Agricultural College, 22 Jinjing Highway, Tianjin 300384, China.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Qie
authors.affiliation: Center for Disease Control and Prevention, PLA Chengdu Military Area Command, Chengdu 610021, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Lu
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Long
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Haiqiang
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Shoudan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Preventive Medicine, Hangzhou Normal University, Hangzhou 311121, China.
authors.forename: Fuzhi
authors.initials: F
authors.lastname: Lian
authors.affiliation: Comparative Medicine Center, Institute of Laboratory Animal Science, Peking Union Medical College (PUMC) and Chinese Academy of Medical Sciences (CAMS), Beijing 100021, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Nutrition, Tianjin Institute of Health and Environmental Medicine, Tianjin 300050, China.
authors.forename: Yugang
authors.initials: Y
authors.lastname: Jiang