title: Luteal changes after treatment with sub-luteolytic doses of prostaglandin (cloprostenol sodium) in cattle.
abstract: This study characterizes the physiological and morphological changes related to partial luteolysis in bovine corpus luteum (CL) after challenges with sub-doses of cloprostenol sodium on Day 6 (D6) of the estrous cycle. Cows (n = 12/treatment) were treated as follows: Control (2 mL, saline, i.m.); 2XPGF (two treatments i.m. 500 μg of cloprostenol sodium 2 h apart) and 1/6PGF (83.3 μg of cloprostenol sodium, i.m., once). Plasma progesterone (P4) concentration, CL volume and blood flow were measured immediately before the treatments, then every 8 h (h) for 48 h. In the Control, P4 concentrations were higher at 48 h than at 0 h. P4 decreased 8h after 2XPGF treatment (P < 0.05), and remained low until the end of the trial. P4 decreased in 1/6PGF between 8 and 16 h (P < 0.05), then began to rebound at 24 h. Luteal volume was higher in Controls at 48 h than at 0 h. Under 1/6PGF, luteal volume decreased at 24 h (P < 0.05) and began to rebound at 32 h. Luteal volume and blood flow were reduced starting at 24 and 32 h, respectively, after 2XPGF treatment (P < 0.05). In this study, we were able to describe the partial luteolysis phenomenon, induced by a treatment of a D6CL with cloprostenol sub-dose.
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Trevisol
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Jair Camargo
authors.initials: JC
authors.lastname: Ferreira
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Camila Louise
authors.initials: CL
authors.lastname: Ackermann
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Flavia Caroline
authors.initials: FC
authors.lastname: Destro
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Wolff Camargo
authors.initials: WC
authors.lastname: Marques Filho
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: Aline Souza
authors.initials: AS
authors.lastname: Carmagos
authors.affiliation: Department of Animal Science, ESALQ, University of São Paulo (USP), Piracicaba, SP, Brazil.
authors.forename: Marcos Vinicius
authors.initials: MV
authors.lastname: Biehl
authors.affiliation: Institute of Animal Science, SAA, Nova Odessa, São Paulo, Brazil.
authors.forename: Jackson Barros
authors.initials: JB
authors.lastname: do Amaral
authors.affiliation: Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: de Figueiredo Pantoja
authors.affiliation: Department of Animal Science, ESALQ, University of São Paulo (USP), Piracicaba, SP, Brazil.
authors.forename: Roberto
authors.initials: R
authors.lastname: Sartori
authors.affiliation: Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista-FMVZ, Unesp, Botucatu, SP, Brazil. Electronic address: jcferreira@fmvz.unesp.br.
authors.forename: João Carlos Pinheiro
authors.initials: JC
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D002417:Cattle; D003008:Cloprostenol / Q000494:pharmacology*; D003338:Corpus Luteum / Q000033:anatomy & histology* / Q000098:blood supply / Q000000981:diagnostic imaging / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008183:Luteal Phase / Q000097:blood / Q000187:drug effects; D003341:Luteolysis / Q000187:drug effects; D008186:Luteolytic Agents / Q000494:pharmacology*; D009929:Organ Size / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D012039:Regional Blood Flow / Q000187:drug effects; D014463:Ultrasonography
chemical_list: D008186:Luteolytic Agents; D003008:Cloprostenol; D011374:Progesterone
keywords: Corpus luteum; Cow; Partial luteolysis