title: Nuclear factor kappa B-dependent Zif268 expression in hippocampus is required for recognition memory in mice.
abstract: Long-term memory formation requires gene expression after acquisition of new information. The first step in the regulation of gene expression is the participation of transcription factors (TFs) such as nuclear factor kappa B (NF-кB), which are present before the neuronal activity induced by training. It was proposed that the activation of these types of TFs allows a second step in gene regulation by induction of immediate-early genes (IEGs) whose protein products are, in turn, TFs. Between these IEGs, zif268 has been found to play a critical role in long-term memory formation and reprocessing after retrieval. Here we found in mice hippocampus that, on one hand, NF-кB was activated 45 min after training in a novel object recognition (NOR) task and that inhibiting NF-кB immediately after training by intrahippocampal administration of NF-кB Decoy DNA impaired NOR memory consolidation. On the other hand, Zif268 protein expression was induced 45 min after NOR training and the administration of DNA antisense to its mRNA post-training impaired recognition memory. Finally, we found that the inhibition of NF-кB by NF-кB Decoy DNA reduced significantly the training-induced Zif268 increment, indicating that NF-кB is involved in the regulation of Zif268 expression. Thus, the present results support the involvement of NF-кB activity-dependent Zif268 expression in the hippocampus during recognition memory consolidation.
authors.affiliation: Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIByNE, CONICET, Ciudad Universitaria, Pab. II, 2do piso, 1428EHA Buenos Aires, Argentina.
authors.forename: Gisela
authors.initials: G
authors.lastname: Zalcman
authors.affiliation: Instituto de Investigación en Biomedicina de Buenos Aires (IBioBA)-CONICET-Partner Institute of the Max Planck Society, Polo Científico Tecnológico, 3er piso, Buenos Aires, Argentina.
authors.forename: Noel
authors.initials: N
authors.lastname: Federman
authors.affiliation: Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIByNE, CONICET, Ciudad Universitaria, Pab. II, 2do piso, 1428EHA Buenos Aires, Argentina.
authors.forename: Verónica
authors.initials: V
authors.lastname: de la Fuente
authors.affiliation: Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IFIByNE, CONICET, Ciudad Universitaria, Pab. II, 2do piso, 1428EHA Buenos Aires, Argentina. Electronic address: aromano@fbmc.fcen.uba.ar.
authors.forename: Arturo
authors.initials: A
authors.lastname: Romano
mesh_terms: D000818:Animals; D051766:Early Growth Response Protein 1 / Q000378:metabolism*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D021641:Recognition, Psychology / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; D016328:NF-kappa B
keywords: Memory consolidation; NF-κB; Transcription factors; Zif268