title: Comparison of backbone dynamics of the type III antifreeze protein and antifreeze-like domain of human sialic acid synthase.
abstract: Antifreeze proteins (AFPs) are found in a variety of cold-adapted (psychrophilic) organisms to promote survival at subzero temperatures by binding to ice crystals and decreasing the freezing temperature of body fluids. The type III AFPs are small globular proteins that consist of one α-helix, three 3(10)-helices, and two β-strands. Sialic acids play important roles in a variety of biological functions, such as development, recognition, and cell adhesion and are synthesized by conserved enzymatic pathways that include sialic acid synthase (SAS). SAS consists of an N-terminal catalytic domain and a C-terminal antifreeze-like (AFL) domain, which is similar to the type III AFPs. Despite having very similar structures, AFL and the type III AFPs exhibit very different temperature-dependent stability and activity. In this study, we have performed backbone dynamics analyses of a type III AFP (HPLC12 isoform) and the AFL domain of human SAS (hAFL) at various temperatures. We also characterized the structural/dynamic properties of the ice-binding surfaces by analyzing the temperature gradient of the amide proton chemical shift and its correlation with chemical shift deviation from random coil. The dynamic properties of the two proteins were very different from each other. While HPLC12 was mostly rigid with a few residues exhibiting slow motions, hAFL showed fast internal motions at low temperature. Our results provide insight into the molecular basis of thermostability and structural flexibility in homologous psychrophilic HPLC12 and mesophilic hAFL proteins.
authors.affiliation: Department of Chemistry and Research Institute of Natural Science, Gyeongsang National University, Jinju, Gyeongnam, 660-701, Republic of Korea.
authors.forename: Yong-Geun
authors.initials: YG
authors.lastname: Choi
authors.forename: Chin-Ju
authors.initials: CJ
authors.lastname: Park
authors.forename: Hee-Eun
authors.initials: HE
authors.lastname: Kim
authors.forename: Yeo-Jin
authors.initials: YJ
authors.lastname: Seo
authors.forename: Ae-Ree
authors.initials: AR
authors.lastname: Lee
authors.forename: Seo-Ree
authors.initials: SR
authors.lastname: Choi
authors.forename: Shim Sung
authors.initials: SS
authors.lastname: Lee
authors.forename: Joon-Hwa
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D021343:Antifreeze Proteins, Type III / Q000378:metabolism* / Q000648:ultrastructure; D003080:Cold Temperature; D018360:Crystallography, X-Ray; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D007652:Oxo-Acid-Lyases / Q000378:metabolism* / Q000648:ultrastructure; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment
chemical_list: D021343:Antifreeze Proteins, Type III; C018730:N-acetylneuraminate synthase; D007652:Oxo-Acid-Lyases