title: Anti-platelet activity of water dispersible curcuminoids in rat platelets.
abstract: Curcuminoids are active principle of turmeric with plethora of health beneficial properties. In this study, we have evaluated for the first time the effect of water dispersible curcuminoids on rat platelet aggregation. Curcuminoids (10-30 µg/mL) significantly inhibited platelet aggregation induced by agonists viz., collagen, ADP and arachidonic acid. Curcuminoids were found to be two-fold more potent than curcumin in inhibiting platelet aggregation. Intracellular curcuminoid concentration was relatively higher than curcumin in rat platelets. Curcuminoids significantly attenuated thromboxane A2 , serotonin levels in rat platelets which play an important role in platelet aggregation. Curcuminoid treatment increased nitric oxide (NO) levels in platelets treated with agonists. Curcuminoids inhibited free radicals such as superoxide anion released from activated platelets, which ultimately inhibits platelet aggregation. Further, curcuminoids inhibited 12-lipoxygenase activity and formation of 12-hydroperoxyeicosatetraenoic acid (12-HPETE) in activated rat platelets which regulates platelet aggregation. The results suggest that curcuminoids have remarkable anti-platelet activity by modulating multiple mechanisms involved in platelet aggregation. Thus curcuminoids may have a therapeutic potential to prevent platelet activation related disorders.
authors.affiliation: Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, Mysore, 570 020, India.
authors.forename: Anikisetty
authors.initials: A
authors.lastname: Maheswaraiah
authors.forename: Lingamallu Jaganmohan
authors.initials: LJ
authors.lastname: Rao
authors.forename: Kamatham Akhilender
authors.initials: KA
authors.lastname: Naidu
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000378:metabolism; D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D015289:Leukotrienes / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D015539:Platelet Activation / Q000187:drug effects; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D013928:Thromboxane A2 / Q000378:metabolism; D014867:Water
chemical_list: D015289:Leukotrienes; D010975:Platelet Aggregation Inhibitors; D059808:Polyphenols; D014867:Water; D013481:Superoxides; D016718:Arachidonic Acid; D009569:Nitric Oxide; D012701:Serotonin; D013928:Thromboxane A2; C021201:12-HPETE; D003094:Collagen; D001092:Arachidonate 12-Lipoxygenase; D003474:Curcumin
keywords: 12-LOX; curcuminoids; nitric oxide; platelet aggregation; superoxide; thromboxane