title: An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat.
abstract: Wheat bread-making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over-expression (Bx7(oe) ), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS-PAGE and normal RP-HPLC procedures failed to separate the two alleles. In the current study, an extensively optimised MALDI-TOF based procedure and a refined DNA based marker for efficiently differentiating Bx7(oe) from normal Bx7 allele were established. Results indicated that the MALDI-TOF procedure is cost effective, high throughput, and proven reliable, while the refined PCR marker only amplifies Bx7(oe) allele, a clear advantage over the previously developed codominant marker.
authors.affiliation: Australia-China Centre for Wheat Improvement, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia; Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Shahidul
authors.initials: S
authors.lastname: Islam
authors.forename: Junhong
authors.initials: J
authors.lastname: Ma
authors.forename: Masood
authors.initials: M
authors.lastname: Anwar
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
authors.forename: Rudi
authors.initials: R
authors.lastname: Appels
authors.forename: Wujun
authors.initials: W
authors.lastname: Ma
mesh_terms: D000483:Alleles; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D005983:Glutens / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin