title: Quantitative proteomic analysis of sub-MIC erythromycin inhibiting biofilm formation of S. suis in vitro.
abstract: Streptococcus suis (S. suis) is a swine pathogen and also a zoonotic agent. Biofilms of S. suis may cause persistent infections by the host immune system and antibiotics. Sub-minimal inhibitory concentration (sub-MIC) of erythromycin can inhibit biofilm formation in bacteria. Here, we performed comparative proteomic analyses of cells at two different conditions: sub-MIC erythromycin treated and nontreated cells. Using iTRAQ strategy, we found some novel proteins that involved in biofilm formation. 79 differentially expressed proteins were identified in sub-MIC erythromycin inhibiting planktonic cell when the protein had both a fold-change of more that a ratio >1.2 or <0.8 (p-value <0.05). Several cell surface proteins (such as Primosomal protein N', l-fucose isomerase, and ABC superfamily ATP binding cassette transporter, membrane protein), as well as those involved in Quorum-sensing, were found to be implicated in biofilm formation. Overall, our results indicated that cell surface proteins played an important role in biofilm formation. Quorum-sensing played a crucial role leading to biofilm formation. ABC superfamily ATP binding cassette transporter, membrane protein and comD might act as channels for erythromycin uptake in Quorum-sensing system. Thus, our data analyzed rough regulatory pathways of biofilm formation that might potentially be exploited to deal with biofilm infections of S. suis. This article is part of a Special Issue entitled: Microbial Proteomics.
  
  
  CONCLUSIONS
  In this study, we identified many proteins involved in cell transport, biological regulation and signal transduction, stress responses and other metabolic processes that were not previously known to be associated with biofilm formation of S. suis and target spot of erythromycin. Therefore, our manuscript represents the most comprehensive analysis of protein profiles of biofilm formation of S. suis inhibited by sub-MIC erythromycin and provides new proteomic information about biofilm formation.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Quan-Yong
authors.initials: QY
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.affiliation: College of Life Science, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Guang-Dong
authors.initials: GD
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang 150030, P.R. China. Electronic address: liyanhua1970a@163.com.
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D017011:Streptococcus suis / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Biofilms; Erythromycin; Proteomics; S. suis; iTRAQ