title: Comparison of international guidelines of dermal absorption tests used in pesticides exposure assessment for operators.
abstract: The number of farmers who have suffered from non-fatal acute pesticide poisoning has been reported to vary from 5.7% to 86.7% in South Korea since 1975. Absorption through the skin is the main route of exposure to pesticides for farmers who operate with them. Several in vitro tests using the skins of humans or animal and in vivo tests using laboratory animals are introduced for the assessment of human dermal absorption level of pesticides. The objective of this study is to evaluate and compare international guidelines and strategies of dermal absorption assessments and to propose unique approaches for applications into pesticide registration process in our situation. Until present in our situation, pesticide exposure level to operator is determined just using default value of 10 as for skin absorption ratio because of data shortage. Dermal absorption tests are requested to get exposure level of pesticides and to ultimately know the safety of pesticides for operators through the comparison with the value of AOEL. When the exposure level is higher than AOEL, the pesticide cannot be approved. We reviewed the skin absorption test guidelines recommended by OECD, EFSA and EPA. The EPA recommends assessment of skin absorption of pesticides for humans through the TPA which includes all the results of in vitro human and animal and animal in vivo skin absorption studies. OECD and EFSA, employ a tiered approach, which the requirement of further study depends on the results of the former stage study. OECD guidelines accept the analysis of pesticide level absorbed through skin without radioisotope when the recovery using the non-labeled method is within 80~120%. Various factors are reviewed in this study, including the origin of skin (gender, animal species and sites of skin), thickness, temperature and, etc., which can influence the integrity of results.
authors.affiliation: Department of Applied Biotoxicology, Hoseo University, Asan, Korea.
authors.forename: Jaehwan
authors.initials: J
authors.lastname: So
authors.affiliation: Department of Applied Biotoxicology, Hoseo University, Asan, Korea.
authors.forename: Junyoung
authors.initials: J
authors.lastname: Ahn
authors.affiliation: Department of Applied Biotoxicology, Hoseo University, Asan, Korea.
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Lee
authors.affiliation: National Institute of Agricultural Science, RDA, Jeonju, Korea.
authors.forename: Kyung-Hun
authors.initials: KH
authors.lastname: Park
authors.affiliation: National Institute of Agricultural Science, RDA, Jeonju, Korea.
authors.forename: Min-Kyoung
authors.initials: MK
authors.lastname: Paik
authors.affiliation: National Institute of Agricultural Science, RDA, Jeonju, Korea.
authors.forename: Mihye
authors.initials: M
authors.lastname: Jeong
authors.affiliation: Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Korea.
authors.forename: Myung-Haing
authors.initials: MH
authors.lastname: Cho
authors.affiliation: Department of Applied Biotoxicology, Hoseo University, Asan, Korea.
authors.forename: Sang-Hee
authors.initials: SH
authors.lastname: Jeong
keywords: AOEL; Dermal absorption; Exposure assessment; Operator safety; Pesticides