title: Bacteria repelling poly(methylmethacrylate-co-dimethylacrylamide) coatings for biomedical devices†Electronic supplementary information (ESI) available: Polymer microarray screening, including analysis of bacterial adhesion by fluorescence microscopy and SEM, and chemical composition of bacteria repelling polymers identified in the screen; polymer synthesis and characterisation; preparation of catheter pieces and solvent studies, and details for confocal imaging/analysis. See DOI: 10.1039/c4tb01129eClick here for additional data file.
abstract: Nosocomial infections due to bacteria have serious implications on the health and recovery of patients in a variety of medical scenarios. Since bacterial contamination on medical devices contributes to the majority of nosocomical infections, there is a need for redesigning the surfaces of medical devices, such as catheters and tracheal tubes, to resist the binding of bacteria. In this work, polyurethanes and polyacrylates/acrylamides, which resist binding by the major bacterial pathogens underpinning implant-associated infections, were identified using high-throughput polymer microarrays. Subsequently, two 'hit' polymers,PA13(poly(methylmethacrylate-co-dimethylacrylamide)) andPA515(poly(methoxyethylmethacrylate-co-diethylaminoethylacrylate-co-methylmethacrylate)), were used to coat catheters and substantially shown to decrease binding of a variety of bacteria (including isolates from infected endotracheal tubes and heart valves from intensive care unit patients). Catheters coated with polymerPA13showed up to 96% reduction in bacteria binding in comparison to uncoated catheters.
authors.affiliation: School of Chemistry , EaStCHEM , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JJ , UK . Email: mark.bradley@ed.ac.uk ; Tel: +44 (0)131 650 4820.
authors.forename: Seshasailam
authors.initials: S
authors.lastname: Venkateswaran
authors.affiliation: School of Chemistry , EaStCHEM , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JJ , UK . Email: mark.bradley@ed.ac.uk ; Tel: +44 (0)131 650 4820.
authors.forename: Mei
authors.initials: M
authors.lastname: Wu
authors.affiliation: School of Biological Sciences , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JF , UK . Email: mp.gallagher@ed.ac.uk ; Tel: +44 (0)131 650 5409.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Gwynne
authors.affiliation: School of Biological Sciences , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JF , UK . Email: mp.gallagher@ed.ac.uk ; Tel: +44 (0)131 650 5409.
authors.forename: Ailsa
authors.initials: A
authors.lastname: Hardman
authors.affiliation: School of Chemistry , EaStCHEM , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JJ , UK . Email: mark.bradley@ed.ac.uk ; Tel: +44 (0)131 650 4820.
authors.forename: Annamaria
authors.initials: A
authors.lastname: Lilienkampf
authors.affiliation: School of Chemistry , EaStCHEM , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JJ , UK . Email: mark.bradley@ed.ac.uk ; Tel: +44 (0)131 650 4820.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Pernagallo
authors.affiliation: School of Biological Sciences , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JF , UK . Email: mp.gallagher@ed.ac.uk ; Tel: +44 (0)131 650 5409.
authors.forename: Garry
authors.initials: G
authors.lastname: Blakely
authors.affiliation: Critical Care , NHS Lothian , Royal Infirmary of Edinburgh , 51 Little France Crescent , Edinburgh , EH16 4SA , UK.
authors.forename: David G
authors.initials: DG
authors.lastname: Swann
authors.affiliation: School of Biological Sciences , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JF , UK . Email: mp.gallagher@ed.ac.uk ; Tel: +44 (0)131 650 5409.
authors.forename: Maurice P
authors.initials: MP
authors.lastname: Gallagher
authors.affiliation: School of Chemistry , EaStCHEM , University of Edinburgh , King's Buildings, West Mains Road , Edinburgh , EH9 3JJ , UK . Email: mark.bradley@ed.ac.uk ; Tel: +44 (0)131 650 4820.
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley