title: Epigallocatechin gallate inhibits human tongue carcinoma cells via HK2‑mediated glycolysis.
abstract: Epigallocatechin gallate (EGCG), one of the major catechins found in green tea, was suggested to play a role as a chemopreventive agent in various human cancer models. In this study, we reported that EGCG has a profound antitumor effect on human tongue carcinoma cells by directly regulating glycolysis. EGCG dose-dependently inhibited anchorage-independent growth and short-term EGCG exposure substantially decreased EGF-induced EGF receptor (EGFR), Akt and ERK1/2 activation, as well as the downregulation of hexokinase 2 (HK2). Furthermore, inhibition of EGCG‑mediated HK2 expression was involved in Akt, but not in ERK1/2 signaling pathway suppression. Overexpression of constitutively activated Akt1 rescued inhibition of EGCG‑induced glycolysis. Moreover, EGCG inhibited HK2 expression on mitochondrial outer membrane and induced apoptosis. In summary, the results suggested that EGCG or a related analogue, may have a role in the management of human tongue carcinoma.
authors.affiliation: Powder Metallurgy Research Institute of Central South University, Changsha, Hunan, P.R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: Changsha Stomatological Hospital, Changsha, Hunan, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pathology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, Hunan, P.R. China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Liu
authors.affiliation: Changsha Stomatological Hospital, Changsha, Hunan, P.R. China.
authors.forename: Zhong-Su
authors.initials: ZS
authors.lastname: Zhou
authors.affiliation: Changsha Stomatological Hospital, Changsha, Hunan, P.R. China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Ultrasonography, The Third Xiangya Hospital of Central South University, Changsha, Hunan, P.R. China.
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Li
authors.affiliation: Powder Metallurgy Research Institute of Central South University, Changsha, Hunan, P.R. China.
authors.forename: Ke-Chao
authors.initials: KC
authors.lastname: Zhou
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D018890:Chemoprevention; D004789:Enzyme Activation / Q000187:drug effects; D066246:ErbB Receptors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects* / Q000502:physiology; D006593:Hexokinase / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D051336:Mitochondrial Membranes / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000096:biosynthesis / Q000378:metabolism; D014062:Tongue Neoplasms / Q000188:drug therapy*
chemical_list: D016588:Anticarcinogenic Agents; D002392:Catechin; C045651:epigallocatechin gallate; D006593:Hexokinase; C512478:EGFR protein, human; D066246:ErbB Receptors; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases