title: [Effects of carbon disulfide inhalation on lipid levels of ApoE gene knockout mice and C57BL/6J mice].
abstract: OBJECTIVE
  To investigate the effects of carbon disulfide (CS(2)) inhalation on the lipid levels of ApoE knockout gene mice and C57BL/6J mice.
  
  
  METHODS
  Fifty-one male ApoE gene knockout mice were randomly divided into four groups: CS(2)-exposed normal diet group, CS(2)-unexposed normal diet group, CS(2)-exposed high-fat diet group, and CS(2)-unexposed high-fat diet group. Fifty male C57BL/6J mice were divided into four groups in the same way. The exposed groups received 1000 mg/m3 CS(2) by static inhalation (5h/d, 5d/w) for four weeks. The weight of each mouse was determined and recorded once a week. On the 14th day of exposure, six mice in each group were randomly selected to measure serum total cholesterol (TC) levels. On the 28th day of exposure, the serum levels of TC and low-density lipoprotein (LDL) in the remaining mice were measured.
  
  
  RESULTS
  The mean weight gain of exposed groups was less than that of the unexposed groups. On the 14th and 28th days of experiment, the TC levels of the CS2-exposed high-fat diet group were significantly higher than those of the CS(2)-unexposed high-fat diet group among ApoE knockout gene mice (P < 0.01 for both). On the 14th day of experiment, the TC levels of the CS(2)-unexposed high-fat diet group were significantly higher than those of the CS(2)-unexposed normal-diet group among C57BL/6J mice group (P < 0.05). On the 28th day of experiment, the LDL levels of the CS(2)-exposed high-fat diet group were significantly higher than those of the CS(2)-unexposed high-fat diet group among ApoE knockout gene mice (P = 0.003).
  
  
  CONCLUSIONS
  CS(2) exposure, high-fat diet, and ApoE gene knockout can elevate blood lipids in mice, thus increasing the risk of atherosclerosis.
authors.affiliation: The School of Public Health, NanjingMedical University, Nanjing 211166, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Chunhui
authors.initials: C
authors.lastname: Ni
authors.forename: Lu
authors.initials: L
authors.lastname: Ding
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.forename: Shanlei
authors.initials: S
authors.lastname: Qiao
authors.forename: Jinglian
authors.initials: J
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Zhong
authors.affiliation: E-mail: zhubl@jscdc.cn.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics*; D050197:Atherosclerosis; D001835:Body Weight; D002246:Carbon Disulfide / Q000633:toxicity*; D059305:Diet, High-Fat / Q000009:adverse effects*; D055786:Gene Knockout Techniques; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000097:blood*; D008077:Lipoproteins, LDL; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D001057:Apolipoproteins E; D008055:Lipids; D008077:Lipoproteins, LDL; D002246:Carbon Disulfide