title: [Intracerelar ventricular injection of the antisense oligonucleotides of ERK5 inhibits neuropathic pain].
abstract: OBJECTIVE
  To explore the effects of extracellular signal-regulated protein kinase 5 signaling pathway in chronic constriction injury (CCI)-induced neuropathic pain above spinal cord level.
  
  
  METHODS
  The CCI model of neuropathic pain was established. Intracerebroventricular injection of antisense oligonucleotides and immunohistochemical labeling technique were employed. And the heat and mechanical hyperalgesia responses were used to examine the affect of ERK5 in CCI-induced neuropathic pain and the expression of p-CREB (cAMP response-element binding protein) in spinal cord.
  
  
  RESULTS
  The mechanical paw withdrawal reflex threshold and thermal reflex latency became significantly lower in CCI-induced mice. Compared with saline group, mechanical and thermal hyperalgesia significantly decreased and the expression of p-CREB protein decreased at spinal cord in oligonucleotide group.
  
  
  CONCLUSIONS
  The ERK5 spinal cord neurons are involved in the regulation of CCI-induced neuropathic pain in mice. And this effect is achieved by adjusting the CREB-dependent gene expression.
authors.affiliation: Department of Anesthesiology, Affiliated First People's Hospital of Soochow University, Suzhou 215006, China.
authors.forename: Quiping
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Anesthesiology, Affiliated First People's Hospital of Soochow University, Suzhou 215006, China. Email: jjcqp@126.com.
authors.forename: Jianping
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein; D006358:Hot Temperature; D006930:Hyperalgesia; D007267:Injections; D048054:Mitogen-Activated Protein Kinase 7 / Q000037:antagonists & inhibitors*; D009437:Neuralgia; D016376:Oligonucleotides, Antisense; D051381:Rats; D015398:Signal Transduction; D013116:Spinal Cord
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D016376:Oligonucleotides, Antisense; D048054:Mitogen-Activated Protein Kinase 7