title: Research to Improve the Efficiency of Double Stereo PCR Microfluidic Chip by Passivating the Inner Surface of Steel Capillary with NOA61.
abstract: In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-µm film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95° to 56°, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.
authors.affiliation: Institute of Laser Engineering, Beijing University of Technology, Beijing, 100124, China. 5777@bjut.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Laser Engineering, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Science, Researching and Training Center, Beijing, 100094, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Applied Sciences, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Kuanxin
authors.initials: K
authors.lastname: Yu
authors.affiliation: Institute of Laser Engineering, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Laser Engineering, Beijing University of Technology, Beijing, 100124, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Science, Researching and Training Center, Beijing, 100094, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
mesh_terms: D000269:Adhesives; D044085:Microfluidics / Q000295:instrumentation*; D016133:Polymerase Chain Reaction / Q000295:instrumentation*
chemical_list: D000269:Adhesives
keywords: Microfluidic chip; NOA 61; Surface modification