title: An integrated passive-flow microfluidic biosensor with organic photodiodes for ultra-sensitive pathogen detection in water.
abstract: This work reports on integrated passive-flow optical microfluidic devices to detect waterborne pathogens in the field. Ring-shaped organic photodiodes were integrated to a capillary-induced flow microfluidic channel for monitoring chemiluminescent sandwich immunoassays enhanced by gold nanoparticles. The integrated device yielded a resolution of 4×10(4) cells/mL for the detection of Legionella pneumophila, which represented a 25-fold improvement over chemiluminescence detection devices employing no gold-nanoparticle enhancement. This work demonstrates the feasibility of a low-cost but highly sensitive lab-on-a-chip device amenable for point-of-use applications.
authors.forename: N M M
authors.initials: NM
authors.lastname: Pires
authors.forename: T
authors.initials: T
authors.lastname: Dong
mesh_terms: D055910:Antibodies, Immobilized / Q000276:immunology; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007118:Immunoassay; D056656:Lab-On-A-Chip Devices; D016952:Legionella pneumophila / Q000276:immunology / Q000302:isolation & purification*; D008163:Luminescent Measurements; D053768:Metal Nanoparticles / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D019095:Point-of-Care Systems; D011108:Polymers / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D055910:Antibodies, Immobilized; D011108:Polymers; D006046:Gold