title: An UPLC-MS/MS method for the analysis of glimepiride and fluoxetine in human plasma.
abstract: A sensitive and rapid ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine glimepiride (GPD) and fluoxetine (FLU) in human plasma using diazepam as the internal standard (IS) simultaneously. The presented method used an Acquity UPLC BEH C18 column for chromatographic separation with tandem mass spectrometric detection on a QTrap5500 mass spectrometer operated in positive ESI mode. The mobile phase is a mixture of acetonitrile and 1% formic acid in water with gradient elution at a flow rate of 0.40mL/min. The GPD, FLU and IS were eluted at 1.46, 1.27 and 1.39min, respectively. The MRM transitions of m/z 491.3→126.3 and m/z 310.5→148.1 were used to quantify for GPD and FLU, respectively. The linearity of this method was found to be within the concentration range of 2.5-300ng/mL and 0.1-20ng/mL for GPD and FLU in human plasma, respectively. The intra- and inter-day precision (RSD%) were less than 10.3% and accuracy (RE%) was within ±7.3%. The matrix effect were 95.3-100.7% for GPD and FLU. GPD and FLU were sufficiently stable under all relevant analytical conditions. The method was also successfully applied to the clinical samples after a single oral dose of 2mg GLP and 40mg FLU in patients.
authors.affiliation: Medical College of Henan University of Science and Technology, 471003 Luoyang, PR China.
authors.forename: Xiangjun
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Nursing of Henan University of Science and Technology, 471003 Luoyang, PR China.
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wang
authors.affiliation: Medical College of Henan University of Science and Technology, 471003 Luoyang, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Medical College of Henan University of Science and Technology, 471003 Luoyang, PR China.
authors.forename: Ying-fei
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Medical College of Henan University of Science and Technology, 471003 Luoyang, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: The First Affiliated Hospital of Wenzhou Medical University, 325000 Wenzhou, PR China. Electronic address: huangxuesun@163.com.
authors.forename: Xue-sun
authors.initials: XS
authors.lastname: Huang
mesh_terms: D005473:Fluoxetine / Q000097:blood*; D006801:Humans; D013453:Sulfonylurea Compounds / Q000097:blood*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013453:Sulfonylurea Compounds; D005473:Fluoxetine; C057619:glimepiride
keywords: Fluoxetine; Glimepiride; Human plasma; UPLC–MS/MS