title: Genotoxic and biochemical changes in Baccharis trimera induced by coal contamination.
abstract: The processing and combustion of coal in thermal power plants release anthropogenic chemicals into the environment. Baccharis trimera is a common plant used in folk medicine that grows readily in soils degraded by coal mining activities. This shrub bioaccumulates metals released into the environment, and thus its consumption may be harmful to health. The purpose of this study was to investigate the phytochemical profile, antioxidant capacity (DPPH), genotoxic (comet assay) and mutagenic potential (CBMN-cyt) in V79 cells of B. trimera aqueous extracts in the coal-mining region of Candiota (Bt-AEC), and in Bagé, a city that does not experience the effects of exposure to coal (Bt-AEB, a reference site). In the comet assay, only Bt-AEC was genotoxic at the highest doses (0.8mg/mL and 1.6mg/mL), compared to the control. For extracts from both areas, mutagenic effects were observed at higher concentrations compared to the control. The cell damage parameters were significantly high in both extracts; however, more striking values were observed for Bt-AEC, up to the dose of 0.8mg/mL. In chemical analysis, no variation was observed in the contents of flavonoids and phenolic compounds, neither the antioxidant activity, which may suggest that DNA damage observed in V79 cells was induced by the presence of coal contaminants absorbed by the plant.
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil; Centro de Ciências da Saúde, Universidade da Região da Campanha (URCAMP), Bagé, Rio Grande do Sul, Brazil.
authors.forename: A P S
authors.initials: AP
authors.lastname: Menezes
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil. Electronic address: juliana.silva@ulbra.br.
authors.forename: J
authors.initials: J
authors.lastname: Da Silva
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil; Centro de Ciências da Saúde, Universidade da Região da Campanha (URCAMP), Bagé, Rio Grande do Sul, Brazil.
authors.forename: R R
authors.initials: RR
authors.lastname: Rossato
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Santos
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: N
authors.initials: N
authors.lastname: Decker
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: Da Silva
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Cruz
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: R R
authors.initials: RR
authors.lastname: Dihl
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Lehmann
authors.affiliation: Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada a Saúde (PPGBioSaude), Universidade Luterana do Brasil, Canoas, Brazil. Electronic address: alexandre.ferraz@ulbra.br.
authors.forename: A B F
authors.initials: AB
authors.lastname: Ferraz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D031175:Baccharis / Q000737:chemistry / Q000254:growth & development; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003031:Coal / Q000633:toxicity*; D003032:Coal Mining; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage; D005419:Flavonoids / Q000032:analysis; D008670:Metals; D009153:Mutagens / Q000302:isolation & purification / Q000633:toxicity*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000302:isolation & purification / Q000633:toxicity*; D011210:Power Plants
chemical_list: D000975:Antioxidants; D003031:Coal; D005419:Flavonoids; D008670:Metals; D009153:Mutagens; D010636:Phenols; D010936:Plant Extracts
keywords: Baccharis trimera; Coal; DNA damage; Genotoxicity; Thermal power plant; V79 cells