title: [Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS].
abstract: To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
authors.affiliation: Institute of Chinese Materia Medica, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Si
authors.forename: Hai-Yu
authors.initials: HY
authors.lastname: Zhao
authors.forename: Bao-Lin
authors.initials: BL
authors.lastname: Bian
authors.forename: Hong-Jie
authors.initials: HJ
authors.lastname: Wang
mesh_terms: D000664:Amphibian Venoms / Q000737:chemistry*; D000818:Animals; D002018:Bufanolides / Q000737:chemistry*; D002025:Bufonidae; D002851:Chromatography, High Pressure Liquid; D053719:Tandem Mass Spectrometry
chemical_list: D000664:Amphibian Venoms; D002018:Bufanolides; C095591:chan su