title: Early embryonic and endometrial regulation of tumor necrosis factor and tumor necrosis factor receptor 2 in the cattle uterus.
abstract: Tumor necrosis factor (TNF) alpha likely mediates embryomaternal communication in mammals. In bovine, we have previously found that the uterine fluid of heifers that carried early embryos shows downregulation in the TNF and nuclear factor κB system. In this work, we assessed the expression of TNF and its receptor TNFR2 in the bovine endometrium and embryos during blastocyst development. Moreover, to explore the endometrial immune response to early embryos, we analyzed the number of CD45 leukocytes in the bovine endometrium. Day 8 endometrium and blastocyst recovered from animals after transfer of Day 5 embryos showed TNF and TNFR2 mRNA transcription and protein colocalization. The presence of embryos increased endometrial TNF and TNFR2 protein, whereas endometrial leukocytes decreased. Blastocysts exposed to the uterine tract had undetectable levels of TNF and lower levels of TNFR2 mRNA. These results suggest that the endometrium might lower the TNF concentration in the blastocyst by (1) regulating TNF secretion into the uterine fluid and (2) inducing decreased TNF and TNFR2 mRNA transcription in the embryo. Thus, TNF and TNFR2 might participate in early embryomaternal communication.
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Correia-Álvarez
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Gómez
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Martín
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Carrocera
authors.affiliation: Unidad de Coordinación de Trasplantes y Terapia Celular, Hospital Universitario Central de Asturias, Oviedo, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Pérez
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France.
authors.forename: N
authors.initials: N
authors.lastname: Peynot
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France.
authors.forename: C
authors.initials: C
authors.lastname: Giraud-Delville
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: J N
authors.initials: JN
authors.lastname: Caamaño
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Balseiro
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France.
authors.forename: O
authors.initials: O
authors.lastname: Sandra
authors.affiliation: INRA, UMR1198 Biologie du Développement et Reproduction, Jouy-en-Josas, France.
authors.forename: V
authors.initials: V
authors.lastname: Duranthon
authors.affiliation: Centro de Biotecnología Animal-SERIDA, Gijón, Asturias, Spain. Electronic address: mmunoz@serida.org.
authors.forename: M
authors.initials: M
authors.lastname: Muñoz
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D047889:Receptors, Tumor Necrosis Factor, Type II; D014409:Tumor Necrosis Factor-alpha; D017493:Leukocyte Common Antigens
keywords: Blastocyst; Bovine; Cytokine; In vivo; Leukocyte