title: [Analytical studies on flavonoids constituents in Apocynum venetom leaves by UPLC-Q-TOF-MS].
abstract: OBJECTIVE
  To establish an ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometric (UPLC-Q-TOF-MS) method for analyzing the flavonoids constituents in Apocynum venetom leaves.
  
  
  METHODS
  The analysis was performed on an Alltima C18 analytical column with a gradient solvent system of acetonitrile-1.0% acetic acid at the flow rate of 0.8 mL/min. The detection wavelength was at 254 nm and the column temperature was at 30 degrees C. Identification and detection were collected under the negative electrospray ion mode using Q-TOF-MS.
  
  
  RESULTS
  11 flavonoids constituents being separated and identified, six of them were detected simultaneously. The results showed that the six detected flavonoids constituents had good linearity between concentrations and peak area (r > 0.9996), and the average recoveries of the method were between 94.3% - 103.3%.
  
  
  CONCLUSIONS
  The method is accurate, reproducible, and can be used for the quality control of Apocynum venetom leaves.
authors.affiliation: Vancheng Health Vocational & Technical College, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Song
authors.forename: Hu
authors.initials: H
authors.lastname: Xu
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Xun-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Feng-Su
authors.initials: FS
authors.lastname: Zhang
authors.forename: Ya
authors.initials: Y
authors.lastname: Hou
authors.forename: Yang
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000097:Acetonitriles; D030010:Apocynum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D018515:Plant Leaves; D012997:Solvents
chemical_list: D000097:Acetonitriles; D005419:Flavonoids; D012997:Solvents; C032159:acetonitrile