title: Differentiation of 3T3-L1 preadipocytes is inhibited under a modified ceiling culture.
abstract: Ceiling culture is an inverted and closed cell culture system which represents a novel method for exploring adipocyte characteristics and function. Although the role of ceiling culture in mature adipocyte dedifferentiation has been extensively studied, its potential effects on preadipocyte differentiation remain unclear. In this study, we established a simplified dish ceiling culture method for 3T3-L1 preadipocytes and showed that our novel ceiling culture method could reproduce the function of the traditional flask ceiling culture. Then, we investigated the effects of ceiling culture on 3T3-L1 preadipocyte differentiation by Oil red O staining and RT-qPCR. The results showed that ceiling culture significantly impaired triglyceride accumulation and adipogenic marker genes expression in 3T3-L1 preadipocytes. These findings suggest that ceiling culture inhibited 3T3-L1 preadipocyte differentiation while inducing mature adipocytes dedifferentiation. Taken together, our data facilitate the understanding of the property of ceiling culture and promote the study of revealing the underlying mechanisms of mature adipocytes dedifferenatiation.
authors.affiliation: Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
authors.forename: Ziyi
authors.initials: Z
authors.lastname: Song
authors.forename: Jia
authors.initials: J
authors.lastname: Cheng
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Gao
authors.forename: Xin'e
authors.initials: X
authors.lastname: Shi
authors.forename: Gongshe
authors.initials: G
authors.lastname: Yang
mesh_terms: D041721:3T3-L1 Cells / Q000166:cytology* / Q000502:physiology*; D017667:Adipocytes / Q000166:cytology* / Q000502:physiology*; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D054337:Cell Dedifferentiation; D002454:Cell Differentiation; D002478:Cells, Cultured; D015536:Down-Regulation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
keywords: 3T3-L1; ceiling culture; dedifferentiation; differentiation; gene expression; hypoxia