title: Akt regulation of Aven contributes to the sensitivity of cancer cells to chemotherapeutic agents.
abstract: In the present study, it was demonstrated that the protein level of the apoptosis inhibitor Aven is regulated by the Akt signaling pathway, evidenced by the observation that Aven levels were significantly increased in MCF7 constitutively active (CA)‑Akt cells and significantly inhibited following treatment with LY294002. This increase in Aven appears not to be mediated by transcriptional regulation and protein stabilization. However, the level of Aven was inversely correlated with the level of cathepsin D, which is a protease responsible for generating the C‑terminal of Aven, ΔN‑Aven, indicating that the level of Aven appears to be regulated by cathepsin D activity. It has previously been reported that ΔN‑Aven is the active form of Aven, which functions as an anti‑apoptotic molecule. Notably, low levels of ΔN‑Aven were detected in MCF7 CA‑Akt cells, which were more sensitive to anticancer drugs. Taken together, the current results suggest that the expression of Aven is regulated by the Akt signaling pathway through cathepsin D activity, which contributes to the sensitivity of cancer cells to chemotherapeutic agents.
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors.forename: Kyoung-Youn
authors.initials: KY
authors.lastname: Han
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors.forename: Jee Won
authors.initials: JW
authors.lastname: Hwang
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors.forename: Gyu-Un
authors.initials: GU
authors.lastname: Bae
authors.affiliation: Natural Products Research Center, Korea Institute of Science and Technology, Gangneung Institute, Gangneung, Gangwon‑do 210‑340, Republic of Korea.
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kim
authors.affiliation: Research Center for Cell Fate Control, College of Pharmacy, Sookmyung Women's University, Seoul 140‑742, Republic of Korea.
authors.forename: Yong Kee
authors.initials: YK
authors.lastname: Kim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D002402:Cathepsin D / Q000494:pharmacology; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D055550:Protein Stability; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014158:Transcription, Genetic
chemical_list: C412506:AVEN protein, human; D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D051017:Apoptosis Regulatory Proteins; D008565:Membrane Proteins; D051057:Proto-Oncogene Proteins c-akt; D002402:Cathepsin D