title: Anionic Lipid, pH-Sensitive Liposome-Gold Nanoparticle Hybrids for Gene Delivery - Quantitative Research of the Mechanism.
abstract: Gene therapy is a potential method for treating a large range of diseases. Gene vectors are widely used in gene therapy for promoting the gene delivery efficiency to the target cells. Here, gold nanoparticles (AuNPs) coated with dimethyldioctadecylammonium bromide (DODAB)/dioleoylphosphatidylethanolamine (DOPE) are synthesized using a facile method for a new gene vector (DODAB/DOPE-AuNPs), which possess 3- and 1.5-fold higher transfection efficiency than those of DODAB-AuNPs and a commercial transfection agent, respectively. Meanwhile, it is nontoxic with concentrations required for effective gene delivery. Imaging and quantification studies of cellular uptake reveal that DOPE increases gene copies in cells, which may be attributed to the smaller size of AuNPs/DNA complexes. The dissociation efficiency of DNA from the endocytic pathway is quantified by incubating with different buffers and investigated directly in the cells. The results suggest that DOPE increases the internalization of AuNPs/DNA complexes and promotes DNA release from early endosomes for the vector is sensitive to the anionic lipid membrane and the decreasing pH along the endocytic pathway. The new vector contains the potential to be the new alternative as gene delivery vector for biomedical applications.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.|University of Chinese Academy of Sciences, Beijing, 100039, China.
authors.forename: Baoji
authors.initials: B
authors.lastname: Du
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.|College of Physics Jilin University, Changchun, Jilin, 130012, China.|WangDepartment of Chemistry and Physics State University of New York at Stony, Brook, Stony Brook, New York, 11794-3400, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000838:Anions; D002021:Buffers; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007273:Injections, Intramuscular; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008807:Mice, Inbred BALB C; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D055672:Static Electricity; D014018:Tissue Distribution / Q000187:drug effects; D014162:Transfection
chemical_list: D000838:Anions; D002021:Buffers; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C094877:dioleoyl phosphatidylethanolamine; C015831:dimethyldioctadecylammonium; D006046:Gold; D004247:DNA
keywords: anion-sensitive; gene delivery; gold nanoparticles; liposome; pH-sensitive