title: High temperature simultaneous saccharification and fermentation of starch from inedible wild cassava (Manihot glaziovii) to bioethanol using Caloramator boliviensis.
abstract: The thermoanaerobe, Caloramator boliviensis was used to ferment starch hydrolysate from inedible wild cassava to ethanol at 60°C. A raw starch degrading α-amylase was used to hydrolyse the cassava starch. During fermentation, the organism released CO2 and H2 gases, and Gas Endeavour System was successfully used for monitoring and recording formation of these gaseous products. The bioethanol produced in stoichiometric amounts to CO2 was registered online in Gas Endeavour software and correlated strongly (R(2)=0.99) with values measured by HPLC. The organism was sensitive to cyanide that exists in cassava flour. However, after acclimatisation, it was able to grow and ferment cassava starch hydrolysate containing up to 0.2ppm cyanide. The reactor hydrogen partial pressure had influence on the bioethanol production. In fed-batch fermentation by maintaining the hydrogen partial pressure around 590Pa, the organism was able to ferment up to 76g/L glucose and produced 33g/L ethanol.
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden; Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar es Salaam, P.O. Box 35179, Dar es Salaam, Tanzania; Tanzania Industrial Research and Development Organization (TIRDO), Kimweri Avenue, TIRDO Complex, Msasani, P.O. Box 23235, Dar-es salaam, Tanzania. Electronic address: ansemoshi@yahoo.co.uk.
authors.forename: Anselm P
authors.initials: AP
authors.lastname: Moshi
authors.affiliation: Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar es Salaam, P.O. Box 35179, Dar es Salaam, Tanzania. Electronic address: kenhosea2@gmail.com.
authors.forename: Ken M M
authors.initials: KM
authors.lastname: Hosea
authors.affiliation: Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, Uvumbuzi Road, Mwalimu J.K. Nyerere Mlimani Campus, University of Dar es Salaam, P.O. Box 35179, Dar es Salaam, Tanzania. Electronic address: elisante@udsm.ac.tz.
authors.forename: Emrode
authors.initials: E
authors.lastname: Elisante
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden. Electronic address: gashaw.mamo@biotek.lu.se.
authors.forename: G
authors.initials: G
authors.lastname: Mamo
authors.affiliation: Division of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden; also at Indienz AB, Annebergs Gård, SE-26873 Billeberga, Sweden. Electronic address: Bo.Mattiasson@biotek.lu.se.
authors.forename: Bo
authors.initials: B
authors.lastname: Mattiasson
mesh_terms: D061249:Batch Cell Culture Techniques; D056804:Biofuels; D001709:Biotechnology / Q000379:methods; D050260:Carbohydrate Metabolism; D002851:Chromatography, High Pressure Liquid; D000068016:Eubacteriales / Q000378:metabolism* / Q000502:physiology; D003486:Cyanides / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005433:Flour; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D002365:Manihot / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D013696:Temperature; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D056804:Biofuels; D003486:Cyanides; D000431:Ethanol; D013213:Starch; D000516:alpha-Amylases; D005947:Glucose
keywords: Bioenergy; Caloramator boliviensis; Gas Endeavour System; Manihot spp.; Thermoanaerobes