title: Permanent proteins in the urine of healthy humans during the Mars-500 experiment.
abstract: Urinary proteins serve as indicators of various conditions in human normal physiology and disease pathology. Using mass spectrometry proteome analysis, the permanent constituent of the urine was examined in the Mars-500 experiment (520 days isolation of healthy volunteers in a terrestrial complex with an autonomous life support system). Seven permanent proteins with predominant distribution in the liver and blood plasma as well as extracellular localization were identified. Analysis of the overrepresentation of the molecular functions and biological processes based on Gene Ontology revealed that the functional association among these proteins was low. The results showed that the identified proteins may be independent markers of the various conditions and processes in healthy humans and that they can be used as standards in determination of the concentration of other proteins in the urine.
authors.affiliation: Institute for Biomedical Problems - Russian Federation State, Scientific Research Center Russian Academy of Sciences, Moscow 123007, Russia , CaDyWEC International Laboratory, Angers Faculty of Medicine, 49045 Angers Cedex 01, France.
authors.forename: Irina M
authors.initials: IM
authors.lastname: Larina
authors.forename: Lyudmila Kh
authors.initials: LKh
authors.lastname: Pastushkova
authors.forename: Evgeny S
authors.initials: ES
authors.lastname: Tiys
authors.forename: Kirill S
authors.initials: KS
authors.lastname: Kireev
authors.forename: Alexey S
authors.initials: AS
authors.lastname: Kononikhin
authors.forename: Natalia L
authors.initials: NL
authors.lastname: Starodubtseva
authors.forename: Igor A
authors.initials: IA
authors.lastname: Popov
authors.forename: Marc-Antoine
authors.initials: MA
authors.lastname: Custaud
authors.forename: Igor V
authors.initials: IV
authors.lastname: Dobrokhotov
authors.forename: Evgeny N
authors.initials: EN
authors.lastname: Nikolaev
authors.forename: Nikolay A
authors.initials: NA
authors.lastname: Kolchanov
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Ivanisenko
mesh_terms: D000328:Adult; D000337:Aerospace Medicine / Q000379:methods; D001798:Blood Proteins / Q000032:analysis / Q000378:metabolism; D064368:Healthy Volunteers; D006801:Humans; D008099:Liver / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D011507:Proteinuria / Q000175:diagnosis*; D040901:Proteomics / Q000379:methods*; D012934:Social Isolation; D013026:Space Flight
chemical_list: D001798:Blood Proteins; D011506:Proteins
keywords: Urinary proteome; associative gene networks; chromatography-mass spectrometry; constant subproteome of urine