title: Probing RNA translocases with DNA.
abstract: For some helicases, it is possible to investigate RNA translocase activity on DNA substrates because the enzyme acts on both substrates. Potassium permanganate (KMnO4) footprinting is a method used to chemically probe the conformation of DNA as well as the binding of proteins. Combining footprinting methods with rapid mixing methods that utilize a chemical quench-flow instrument can enable tracking of the translocase with nucleotide resolution.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR, USA.
authors.forename: Kimberly A
authors.initials: KA
authors.lastname: Reynolds
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Raney
authors.forename: Kevin D
authors.initials: KD
authors.lastname: Raney
mesh_terms: D004247:DNA / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011196:Potassium Permanganate / Q000737:chemistry; D020365:RNA Helicases / Q000378:metabolism*
chemical_list: D011196:Potassium Permanganate; D004247:DNA; D020365:RNA Helicases