title: Activation of the ubiquitin proteasome pathway by silk fibroin modified chitosan nanoparticles in hepatic cancer cells.
abstract: Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation.
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. myang@mail.kmuh.org.tw.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan. twchung@ms.ym.edu.tw.
authors.forename: Tze-Wen
authors.initials: TW
authors.lastname: Chung
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan. eshan5413@gmail.com.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Lu
authors.affiliation: Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. 1010.yiling@gmail.com.
authors.forename: Yi-Ling
authors.initials: YL
authors.lastname: Chen
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan. wanchi@cc.kmu.edu.tw.
authors.forename: Wan-Chi
authors.initials: WC
authors.lastname: Tsai
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan. jongsb@kmu.edu.tw.
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. yuanssf@kmu.edu.tw.
authors.forename: Shyng-Shiou
authors.initials: SS
authors.lastname: Yuan
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. liaopc@mail.ncku.edu.tw.
authors.forename: Pao-Chi
authors.initials: PC
authors.lastname: Liao
authors.affiliation: Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. pclin@mail.nsysu.edu.tw.
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.affiliation: Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan. yctyan@kmu.edu.tw.
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D001672:Biocompatible Materials / Q000378:metabolism; D002460:Cell Line; D048271:Chitosan / Q000378:metabolism*; D004337:Drug Carriers / Q000378:metabolism*; D005349:Fibroins / Q000378:metabolism*; D056945:Hep G2 Cells; D006801:Humans; D053758:Nanoparticles / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D025801:Ubiquitin; D005349:Fibroins; D048271:Chitosan; D046988:Proteasome Endopeptidase Complex