title: High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors.
abstract: Choroid plexus tumors are rare neoplasms that mainly affect children. They include papillomas, atypical papillomas, and carcinomas. Detailed genetic studies are rare, and information about their molecular pathogenesis is limited. Molecular inversion probe analysis is a hybridization-based method that represents a reliable tool for the analysis of highly fragmented formalin-fixed paraffin-embedded tissue-derived DNA. Here, analysis of 62 cases showed frequent hyperdiploidy in papillomas and atypical papillomas that appeared very similar in their cytogenetic profiles. In contrast, carcinomas showed mainly losses of chromosomes. Besides recurrent focal chromosomal gains common to all choroid plexus tumors, including chromosome 14q21-q22 (harboring OTX2), chromosome 7q22 (LAMB1), and chromosome 9q21.12 (TRPM3), Genomic Identification of Significant Targets in Cancer analysis uncovered focal alterations specific for papillomas and atypical papillomas (e.g. 7p21.3 [ARL4A]) and for carcinomas (16p13.3 [RBFOX1] and 6p21 [POLH, GTPBP2, RSPH9, and VEGFA]). Additional RNA expression profiling and gene set enrichment analysis revealed greater expression of cell cycle-related genes in atypical papillomas in comparison with that in papillomas. These findings suggest that atypical papillomas represent an immature variant of papillomas characterized by increased proliferative activity, whereas carcinomas seem to represent a genetically distinct tumor group.
authors.affiliation: From the Institute of Neuropathology, University of Bonn Medical Center, Bonn (ASJ, MG, DD, AzM, TP); Department of Pediatric Neurosurgery, Asklepios Children's Clinic Sankt Augustin, Sankt Augustin (MM-J); Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg (SH, UK); and Department of Cell Biology (Tumor Research), University of Duisburg-Essen, Essen (LK-H), Germany; and Department of Pediatric Hematology/Oncology and Blood and Marrow Transplantation, Cleveland Clinic, Cleveland, Ohio (JEW).
authors.forename: Anna Sophia
authors.initials: AS
authors.lastname: Japp
authors.forename: Marco
authors.initials: M
authors.lastname: Gessi
authors.forename: Martina
authors.initials: M
authors.lastname: Messing-Jünger
authors.forename: Dorota
authors.initials: D
authors.lastname: Denkhaus
authors.forename: Anja
authors.initials: A
authors.lastname: Zur Mühlen
authors.forename: Johannes Ernst
authors.initials: JE
authors.lastname: Wolff
authors.forename: Stefan
authors.initials: S
authors.lastname: Hartung
authors.forename: Uwe
authors.initials: U
authors.lastname: Kordes
authors.forename: Ludger
authors.initials: L
authors.lastname: Klein-Hitpass
authors.forename: Torsten
authors.initials: T
authors.lastname: Pietsch
mesh_terms: D000293:Adolescent; D000328:Adult; D002277:Carcinoma / Q000235:genetics*; D002648:Child; D002675:Child, Preschool; D016545:Choroid Plexus Neoplasms / Q000235:genetics*; D002869:Chromosome Aberrations; D002877:Chromosomes, Human; D005260:Female; D005500:Follow-Up Studies; D023281:Genomics; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D051857:Otx Transcription Factors / Q000235:genetics; D020288:Papilloma, Choroid Plexus / Q000235:genetics*; D050053:TRPM Cation Channels / Q000235:genetics; D059467:Transcriptome; D055815:Young Adult
chemical_list: C497659:OTX2 protein, human; D051857:Otx Transcription Factors; D050053:TRPM Cation Channels; C475667:TRPM3 protein, human