title: Preparation of photocrosslinked fish elastin polypeptide/microfibrillated cellulose composite gels with elastic properties for biomaterial applications.
abstract: Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N'-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress-strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity.
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. shinya.y08232@gmail.com.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yano
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. megumi.m07232@gmail.com.
authors.forename: Megumi
authors.initials: M
authors.lastname: Mori
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. teramoto.naozumi@it-chiba.ac.jp.
authors.forename: Naozumi
authors.initials: N
authors.lastname: Teramoto
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. makoto.i10230@gmail.com.
authors.forename: Makoto
authors.initials: M
authors.lastname: Iisaka
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. natsumi.s09231@gmail.com.
authors.forename: Natsumi
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. masanari.n11231@gmail.com.
authors.forename: Masanari
authors.initials: M
authors.lastname: Noto
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. yasuko.k05230@gmail.com.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Kaimoto
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. masashi.k06230@gmail.com.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kakimoto
authors.affiliation: Research & Development Division, Hayashikane Sangyo Co., Ltd., 2-4-8 Yamato-machi, Shimonoseki, Yamaguchi 750-8608, Japan. myamada@hayashikane.co.jp.
authors.forename: Michio
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Research & Development Division, Hayashikane Sangyo Co., Ltd., 2-4-8 Yamato-machi, Shimonoseki, Yamaguchi 750-8608, Japan. esiratsuti@hayashikane.co.jp.
authors.forename: Eri
authors.initials: E
authors.lastname: Shiratsuchi
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. shimasaki.toshiaki@it-chiba.ac.jp.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Shimasaki
authors.affiliation: Department of Life and Environmental Sciences, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan. mitsuhiro.shibata@p.chibakoudai.jp.
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Shibata
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D002482:Cellulose / Q000138:chemical synthesis / Q000737:chemistry*; D004548:Elasticity; D004549:Elastin / Q000737:chemistry*; D005782:Gels / Q000138:chemical synthesis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D055668:Photochemical Processes; D017550:Spectroscopy, Fourier Transform Infrared; D014413:Tuna / Q000378:metabolism
chemical_list: D001672:Biocompatible Materials; D005782:Gels; D010455:Peptides; D002482:Cellulose; D004549:Elastin