title: Two novel NAC transcription factors regulate gene expression and flowering time by associating with the histone demethylase JMJ14.
abstract: The histone demethylase JMJ14 catalyzes histone demethylation at lysine 4 of histone 3 and is involved in transcriptional repression and flowering time control in Arabidopsis. Here, we report that JMJ14 is physically associated with two previously uncharacterized NAC transcription factors, NAC050 and NAC052. The NAC050/052-RNAi plants and the CRISPR-CAS9-mediated nac050/052 double mutant plants show an early flowering phenotype, which is similar to the phenotype of jmj14, suggesting a functional association between JMJ14 and NAC050/052. RNA-seq data indicated that hundreds of common target genes are co-regulated by JMJ14 and NAC50/052. Our ChIP analysis demonstrated that JMJ14 and NAC050 directly bind to co-upregulated genes shared in jmj14 and NAC050/052-RNAi, thereby facilitating H3K4 demethylation and transcriptional repression. The NAC050/052 recognition DNA cis-element was identified by an electrophoretic mobility shift assay at the promoters of its target genes. Together, our study identifies two novel NAC transcription repressors and demonstrates that they are involved in transcriptional repression and flowering time control by associating with the histone demethylase JMJ14.
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Ning
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Ze-Yang
authors.initials: ZY
authors.lastname: Ma
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Huan-Wei
authors.initials: HW
authors.lastname: Huang
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Mo
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Ting-ting
authors.initials: TT
authors.lastname: Zhao
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cai
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: She
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Ligeng
authors.initials: L
authors.lastname: Ma
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China hexinjian@nibs.ac.cn.
authors.forename: Xin-Jian
authors.initials: XJ
authors.lastname: He
mesh_terms: D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D035264:Flowers / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D056466:Histone Demethylases / Q000378:metabolism*; D013058:Mass Spectrometry; D014157:Transcription Factors / Q000502:physiology*; D020798:Two-Hybrid System Techniques
chemical_list: D014157:Transcription Factors; D056466:Histone Demethylases