title: Iopromide in combination with IFN-γ induces the activation of HMC-1 cells via IL-4 and MCP-1 expression.
abstract: In this study, we investigated whether IFN-γ has a role in contrast-medium-induced adverse reactions. Iopromide, a nonionic iodinated contrast agent, slightly induced mast cell proliferation and significantly increased the expression of IL-4 and MCP-1 at low doses. The pretreatment of cells with IFN-γ dramatically increased the expression of iopromide-induced IL-4 and MCP-1. An evaluation of mast cell activator secretion revealed that IFN-γ- or IL-4-pretreated HMC-1 cells released dramatically increased levels of β-hexosaminidase and histamine when stimulated with iopromide. We also found that the migration of EoL-1 and THP-1 cells was significantly increased in culture conditions with iopromide-stimulated IL-4-pretreated HMC-1 cells. Taken together, our findings suggest that measuring IFN-γ or IL-4 levels in serum would be helpful as a potential biomarker of adverse patient reactions and that blocking IFN-γ or IL-4 may be crucial in preventing the delayed allergy-like reaction induced by contrast medium in patients with various diseases.
authors.affiliation: Advanced Research Center for Multiple Myeloma, Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 614-735, Republic of Korea.
authors.forename: Hae-Yun
authors.initials: HY
authors.lastname: Cho
authors.affiliation: Radiology, Busan Paik Hospital, Inje University College of Medicine, Busan 614-735, Republic of Korea.
authors.forename: Seok Jin
authors.initials: SJ
authors.lastname: Choi
authors.affiliation: Department of Oral and Maxillofacial Surgery, Haeundae Paik Hospital, Inje University, Busan 612-896, Republic of Korea.
authors.forename: Soo-Woon
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Emergency Medicine, Busan Paik Hospital, Inje University College of Medicine, Busan 612-896, Republic of Korea.
authors.forename: Yang Weon
authors.initials: YW
authors.lastname: Kim
authors.affiliation: Institute of Environmental and Occupational Medicine, Department of Occupational and Environmental Medicine, Busan Paik Hospital, Inje University, Busan 614-735, Republic of Korea.
authors.forename: Chae Kwan
authors.initials: CK
authors.lastname: Lee
authors.affiliation: Advanced Research Center for Multiple Myeloma, Department of Microbiology and Immunology, College of Medicine, Inje University, Busan 614-735, Republic of Korea; Bio-Marker Research Center for Personalized Therapy, College of Medicine, Inje University, Busan 614-735, Republic of Korea. Electronic address: swlee@inje.ac.kr.
authors.forename: Soo-Woong
authors.initials: SW
authors.lastname: Lee
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology*; D003287:Contrast Media / Q000008:administration & dosage / Q000494:pharmacology*; D006632:Histamine / Q000032:analysis / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology*; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology*; D007472:Iohexol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D012313:RNA / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D001619:beta-N-Acetylhexosaminidases / Q000032:analysis / Q000276:immunology
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D003287:Contrast Media; D015847:Interleukin-4; D007472:Iohexol; D012313:RNA; C038192:iopromide; D006632:Histamine; D007371:Interferon-gamma; D001619:beta-N-Acetylhexosaminidases
keywords: Cell migration; HMC-1 cells; IFN-γ; IL-4; Iopromide; MCP-1