title: Emcibacter nanhaiensis gen. nov. sp. nov., isolated from sediment of the South China Sea.
abstract: A new aerobic, Gram stain-negative, oxidase- and catalase- positive, ovoid or rod-shaped bacterial strain, designated HTCJW17(T), was isolated from a sediment collected from the South China Sea and subjected to a polyphasic taxonomic characterization. The isolate forms small, creamy-white, opaque and circular colonies on agar plates. Growth occurs between 15 and 45 °C, 1-7 % (w/v) NaCl and pH 6-9. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HTCJW17(T) belongs to the family Kordiimonadaceae of the order Kordiimonadales, with highest sequence similarity to Kordiimonas gwangyangensis GW14-5(T) (91.1 %). The major fatty acids were identified to be summed feature 8 (C18:1 ω7c/ω6c; 56.6 %), C14:0 (8.5 %), C14:0 2-OH (8.4 %), summed feature 3 (C16:1 ω7c/C16:1 ω6c; 6.2 %), and C16:0 (5.2 %). The polar lipids were determined to be phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, six unidentified glycolipids and two unidentified phospholipids. The respiratory quinone was identified as ubiquinone Q-10. The genomic DNA G+C content was determined to be 56.3 mol%. On the basis of genotypic, chemotaxonomic and phenotypic distinctness, we propose a novel genus, Emcibacter gen. nov., with Emcibacter nanhaiensis sp. nov. as the type species. The type strain is HTCJW17(T) (=CGMCC 1.12471(T) = LMG 27419(T) = MCCC 1A06723(T)).
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, 361005, China.
authors.forename: Xiupian
authors.initials: X
authors.lastname: Liu
authors.forename: Guangyu
authors.initials: G
authors.lastname: Li
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.forename: Fengqin
authors.initials: F
authors.lastname: Sun
authors.forename: Yaping
authors.initials: Y
authors.lastname: Du
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000332:Aerobiosis; D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride