title: Cytotoxicity of synthesized Iron Oxide nanoparticles: toward novel biomarkers of colon cancer.
abstract: In this paper we present the preliminary results of a novel biological analysis platform for early colon cancer detection using magnetic separation of magnetized markers. The platform consists of a microfluidic structure integrated with biosensors. Super-Paramagnetic Iron Oxide nanoparticles (SPIO-NPs) were functionalized with purified DNA Aptamer and their synthesis is described. In this paper, we also present the physicochemical results of the synthesized SPIO/Au-NPs characterized by TEM and XRD. Toxicity of our synthesized biomarkers on HCT116 cell line is discussed. Based on our findings, a concentration of 1mg/ml of our biomarkers added to 5 × 105 cells per well has no effect the viability of the human cells even after 24 hours.
authors.forename: M Ahmadzadeh
authors.initials: MA
authors.lastname: Raji
authors.forename: M
authors.initials: M
authors.lastname: Amara
authors.forename: G
authors.initials: G
authors.lastname: Amoabediny
authors.forename: P
authors.initials: P
authors.lastname: Tajik
authors.forename: A
authors.initials: A
authors.lastname: Barin
authors.forename: S
authors.initials: S
authors.lastname: Magierowski
authors.forename: E
authors.initials: E
authors.lastname: Ghafar-Zadeh
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis*; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D010316:Particle Size
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D058185:Magnetite Nanoparticles; D052203:Ferrosoferric Oxide