title: MicroRNA expression profiling of lactating mammary gland in divergent phenotype swine breeds.
abstract: MicroRNA (miRNA) plays a key role in development and specific biological processes, such as cell proliferation, differentiation, and apoptosis. Extensive studies of mammary miRNAs have been performed in different species and tissues. However, little is known about porcine mammary gland miRNAs. In this study, we report the identification and characterization of miRNAs in the lactating mammary gland in two distinct pig breeds, Jinhua and Yorkshire. Many miRNAs were detected as significantly differentially expressed between the two libraries. Among the differentially expressed miRNAs, many are known to be related to mammary gland development and lactation by interacting with putative target genes in previous studies. These findings suggest that miRNA expression patterns may contribute significantly to target mRNA regulation and influence mammary gland development and peak lactation performance. The data we obtained provide useful information about the roles of miRNAs in the biological processes of lactation and the mechanisms of target gene expression and regulation.
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. pennypeng1987@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. zjs4939@126.com.
authors.forename: Jun-Sheng
authors.initials: JS
authors.lastname: Zhao
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. 06dwkxsyf@zju.edu.cn.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Shen
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. maohaiguang@163.com.
authors.forename: Hai-Guang
authors.initials: HG
authors.lastname: Mao
authors.affiliation: College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. nyxu@zju.edu.cn.
authors.forename: Ning-Ying
authors.initials: NY
authors.lastname: Xu
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D013552:Swine
chemical_list: D035683:MicroRNAs