title: Ophiopogon polysaccharide liposome can enhance the non-specific and specific immune response in chickens.
abstract: The purpose of this study is to investigate the immune-enhancing activity of ophiopogon polysaccharide liposome (OPL). In non-specific immune response experiment, the phagocytosis and cytokines secretion of peritoneal macrophages in vitro and in vivo were performed. In specific immune response experiment, the activity of OPL was measured on chickens which were vaccinated with Newcastle disease (ND) vaccine and then challenged with ND virus at 49-old-day. The results showed that OPL could significantly promote the phagocytosis of macrophages and induce the secretion of IL-2 and IL-6 in vitro; OPL at high and medium doses could significantly improve the phagocytosic index, promote lymphocyte proliferation, increase the proportion of T lymphocyte subpopulations (CD4(+) and CD8(+)), enhance antibody titer and improve the protective rate in vivo. Moreover, its efficacy was significantly better than ophiopogon polysaccharide (OP). These results indicated that the immune-enhancing activity of OP was significantly improved after encapsulated with liposome.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Medicinal Engineering Department of Henan University of Animal Husbandry and Economy, Zhengzhou 450011, Henan, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Ma
authors.affiliation: Wuhan Huisheng Biotechnology Group, Wuhan 430042, Hubei, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Hou
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Guo
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, PR China. Electronic address: dwtong@nwsuaf.edu.cn.
authors.forename: Dewen
authors.initials: D
authors.lastname: Tong
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens / Q000276:immunology* / Q000821:virology; D007109:Immunity / Q000187:drug effects; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008081:Liposomes / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009521:Newcastle Disease / Q000276:immunology / Q000821:virology; D009522:Newcastle disease virus / Q000187:drug effects; D031441:Ophiopogon / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000276:immunology*; D020011:Protective Agents / Q000494:pharmacology
chemical_list: D000906:Antibodies; D007376:Interleukin-2; D015850:Interleukin-6; D008081:Liposomes; D011134:Polysaccharides; D020011:Protective Agents
keywords: Immune-enhancing activity; Non-specific immune response; Ophiopogon polysaccharide liposome; Specific immune response