title: Kinetic study of Candida antarctica lipase B immobilization using poly(methyl methacrylate) nanoparticles obtained by miniemulsion polymerization as support.
abstract: With the objective to obtain immobilized Candida antarctica lipase B (CalB) with good activity and improved utilization rate, this study evaluated the influence of enzyme and crodamol concentrations and initiator type on the CalB enzyme immobilization in nanoparticles consisting of poly(methyl methacrylate) (PMMA) obtained by miniemulsion polymerization. The kinetic study of immobilized CalB enzyme in PMMA nanoparticles was evaluated in terms of monomer conversion, particle size, zeta potential, and relative activity. The optimum immobilization condition for CalB was compared with free enzyme in the p-NPL hydrolysis activity measurement. Results showed a higher CalB enzyme stability after 20 hydrolysis cycles compared with free CalB enzyme; in particular, the relative immobilized enzyme activity was maintained up to 40%. In conclusion, PMMA nanoparticles proved to be a good support for the CalB enzyme immobilization and may be used as a feasible alternative catalyst in industrial processes.
authors.affiliation: Chemical Engineering and Food Engineering Department, Federal University of Santa Catarina (UFSC), P.O. Box 476, Florianópolis, SC, 88040-900, Brazil, ale_valerio1@yahoo.com.br.
authors.forename: Alexsandra
authors.initials: A
authors.lastname: Valério
authors.forename: Gabrieli
authors.initials: G
authors.lastname: Nicoletti
authors.forename: Eliane P
authors.initials: EP
authors.lastname: Cipolatti
authors.forename: Jorge L
authors.initials: JL
authors.lastname: Ninow
authors.forename: Pedro H H
authors.initials: PH
authors.lastname: Araújo
authors.forename: Cláudia
authors.initials: C
authors.lastname: Sayer
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D002175:Candida / Q000201:enzymology; D002384:Catalysis; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D007700:Kinetics; D008049:Lipase / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D019904:Polymethyl Methacrylate / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D019904:Polymethyl Methacrylate; D008049:Lipase; C431349:lipase B, Candida antarctica