title: Nanotube array method for studying lipid-induced conformational changes of a membrane protein by solid-state NMR.
abstract: Anodic aluminum oxide substrates with macroscopically aligned homogeneous nanopores of 80 nm in diameter enable two-dimensional, solid-state nuclear magnetic resonance studies of lipid-induced conformational changes of uniformly (15)N-labeled Pf1 coat protein in native-like bilayers. The Pf1 helix tilt angles in bilayers composed of two different lipids are not entirely governed by the membrane thickness but could be rationalized by hydrophobic interactions of lysines at the bilayer interface. The anodic aluminum oxide alignment method is applicable to a broader repertoire of lipids versus bicelle bilayer mimetics currently employed in solid-state nuclear magnetic resonance of oriented samples, thus allowing for elucidation of the role played by lipids in shaping membrane proteins.
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina.
authors.forename: Antonin
authors.initials: A
authors.lastname: Marek
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina.
authors.forename: Sergey
authors.initials: S
authors.lastname: Milikisiyants
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina. Electronic address: alex_nevzorov@ncsu.edu.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Nevzorov
authors.affiliation: Department of Chemistry, North Carolina State University, Raleigh, North Carolina. Electronic address: alex_smirnov@ncsu.edu.
authors.forename: Alex I
authors.initials: AI
authors.lastname: Smirnov
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry; D005240:Feasibility Studies; D016018:Least-Squares Analysis; D008051:Lipid Bilayers / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D043942:Nanotubes / Q000737:chemistry*; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D010715:Phosphatidylglycerols / Q000737:chemistry; D010759:Phosphorus Isotopes; D017433:Protein Structure, Secondary
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D009587:Nitrogen Isotopes; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010759:Phosphorus Isotopes; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol; C017251:1,2-oleoylphosphatidylcholine; D000537:Aluminum Oxide; D004134:Dimyristoylphosphatidylcholine