title: Evaluation of immunological interaction between spermatozoa and fallopian tube epithelial cells.
abstract: Toll-like receptors (TLR) are one of the major compartments of innate immune system. It was revealed that the TLR have relevance in ovulation, sperm capacitation and fertilisation. So, in this study, the expression of TLR, their adaptor molecules and cytokines in human fallopian tube cell line under the effect of human normal spermatozoa was evaluated. TLR mRNA and protein were evaluated in OE-E6/E7 cell line. Semen samples from 10 donors were collected and co-incubated with OE-E6/E7 cell line and used as sperm group, and cell line without spermatozoa was used as control group. Afterwards, the level of TLR, their adaptor molecule and cytokine mRNA expression was compared using qPCR in sperm and control groups, and supernatant was used for ELISA. To determine whether elevated cytokine reaction to spermatozoa in OE-E6/E7 cell line is mediated via TLR, TLR3 function-blocking antibody was used. OE-E6/E7 cell line expressed TLR1-6 genes and proteins. TLR expressions, especially TLR3 and TLR5, in OE-E6/E7 cell line under the effect of spermatozoa were significantly higher. Also, levels of adaptor molecules and cytokine production were increased in sperm group than in control group (P < 0.05). So, it may be hypothesised that TLR are essential for spermatozoa and fallopian tube immunological interaction and for preparing safe environment for important events in fallopian tube.
authors.affiliation: Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Zandieh
authors.affiliation: Obstetrics and Gynecology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Ashrafi
authors.affiliation: Anatomy Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: B
authors.initials: B
authors.lastname: Jameie
authors.affiliation: Valie-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Amanpour
authors.affiliation: Department of Immunology, Faculty of Medicine, Shaheed Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: N
authors.initials: N
authors.lastname: Mosaffa
authors.affiliation: Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Salman Yazdi
authors.affiliation: Academic Unit of Reproductive and Developmental Medicine, University of Sheffield, Sheffield, UK.
authors.forename: A
authors.initials: A
authors.lastname: Pacey
authors.affiliation: Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Aflatoonian
mesh_terms: D016207:Cytokines / Q000502:physiology; D004848:Epithelium / Q000276:immunology / Q000502:physiology; D005187:Fallopian Tubes / Q000276:immunology* / Q000502:physiology; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000502:physiology; D015850:Interleukin-6 / Q000502:physiology; D016209:Interleukin-8 / Q000502:physiology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D013094:Spermatozoa / Q000276:immunology* / Q000502:physiology; D051193:Toll-Like Receptors / Q000502:physiology; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D051193:Toll-Like Receptors; D014409:Tumor Necrosis Factor-alpha; D016899:Interferon-beta
keywords: Fallopian tube; innate immunity; spermatozoa; toll-like receptor