title: Construction of Escherichia Coli Cell Factories for Production of Organic Acids and Alcohols.
abstract: Production of bulk chemicals from renewable biomass has been proved to be sustainable and environmentally friendly. Escherichia coli is the most commonly used host strain for constructing cell factories for production of bulk chemicals since it has clear physiological and genetic characteristics, grows fast in minimal salts medium, uses a wide range of substrates, and can be genetically modified easily. With the development of metabolic engineering, systems biology, and synthetic biology, a technology platform has been established to construct E. coli cell factories for bulk chemicals production. In this chapter, we will introduce this technology platform, as well as E. coli cell factories successfully constructed for production of organic acids and alcohols.
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Area, Tianjin, 300308, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Area, Tianjin, 300308, China.
authors.forename: Xinna
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Area, Tianjin, 300308, China.|University of Chinese Academy of Sciences, Tianjin, China.
authors.forename: Zaigao
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. zhang_xl@tib.cas.cn.|Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Area, Tianjin, 300308, China. zhang_xl@tib.cas.cn.
authors.forename: Xueli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000438:Alcohols / Q000378:metabolism*; D002264:Carboxylic Acids / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*
chemical_list: D000438:Alcohols; D002264:Carboxylic Acids
keywords: Bulk chemicals; Cell factories; Escherichia coli; Metabolic engineering; Synthetic biology