title: Spontaneous immortalization of mouse liver sinusoidal endothelial cells.
abstract: The spontaneous immortalization of cells in vitro is a rare event requiring genomic instability, such as alterations in chromosomes and mutations in genes. In the present study, we report a spontaneously immortalized liver sinusoidal endothelial cell (LSEC) line generated from mouse liver. These immortalized LSECs showed typical LSEC characteristics with the structure of transcellular fenestrations, the expression of von Willebrand factor (VWF) and the ability to uptake DiI-acetylated-low density lipoprotein (DiI-Ac-LDL). However, these immortalized LSECs lost the ability to form capillary-like structures, and showed clonal and multilayer growth without contact inhibition. Moreover, their proliferation rate increased with the increase in the number of passages. In addition, these cells obained the expression of CD31 and desmin, and showed an upregulation of p53 protein expression; however, their karyotype was normal, and they could not form colonies in soft agar or tumors in SCID mice. In conclusion, in the present study, we successfully established a spontaneously immortalized LSEC line.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, Xi'an, Shanxi 710004, P.R. China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Na
authors.initials: N
authors.lastname: Xiao
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Adult Stem Cells, Institute of Reproduction and Stem Cell Engineering, Central South University, Changsha, Hunan 410078, P.R. China.
authors.forename: Lamei
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000818:Animals; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D016922:Cellular Senescence; D042783:Endothelial Cells / Q000378:metabolism*; D059785:Karyotype; D008099:Liver / Q000166:cytology*; D051379:Mice; D010641:Phenotype; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D016159:Tumor Suppressor Protein p53