title: Temperature dependence of production of structured triacylglycerols in the alga Trachydiscus minutus.
abstract: This study describes the identification of regioisomers and enantiomers of triacylglycerols of C20 polyunsaturated fatty acids (PUFAs) in the alga Trachydiscus minutus cultivated at different temperatures using reversed- and chiral-phase liquid chromatography-mass spectrometry. The use of the two different phases contributes to ready identification, both qualitative and semiquantitative, of regioisomers and enantiomers of triacylglycerols containing eicosapentaenoic and arachidonic in the molecule. The ratio of regioisomers and enantiomers of triacylglycerols (TAG) depends on the temperature of cultivation; with lowering temperature the proportion of the achiral TAG increases and the enantiomer ratio diverges from 1:1.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Řezanka
authors.affiliation: Institute of Botany, Academy of Sciences of the Czech Republic, Biorefinery Res. Centre of Competence, Dukelská 135, 379 82 Třeboň, Czech Republic.
authors.forename: Jaromír
authors.initials: J
authors.lastname: Lukavský
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Sigler
authors.affiliation: Department of Ecology, Faculty of Science, Charles University in Prague, Viničná 7, 128 44 Prague 2, Czech Republic.
authors.forename: Linda
authors.initials: L
authors.lastname: Nedbalová
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Cell Cycles of Algae, Centre Algatech, Opatovický mlýn 237, 379 81 Třeboň, Czech Republic.
authors.forename: Milada
authors.initials: M
authors.lastname: Vítová
mesh_terms: D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D013237:Stereoisomerism; D058009:Stramenopiles / Q000737:chemistry*; D013696:Temperature; D014280:Triglycerides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D005231:Fatty Acids, Unsaturated; D014280:Triglycerides
keywords: Alga; Chiral-LC/APCI-MS; Eustigmatophyceae; Structured triacylglycerols; Temperature; Trachydiscus minutus