title: Sexual recombination in Aspergillus tubingensis.
abstract: Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.
authors.affiliation: Center for Integrated Fungal Research, Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Olarte
authors.affiliation: National Peanut Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Dawson, Georgia 39842.
authors.forename: Bruce W
authors.initials: BW
authors.lastname: Horn
authors.affiliation: Center for Integrated Fungal Research, Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695.
authors.forename: Rakhi
authors.initials: R
authors.lastname: Singh
authors.affiliation: Center for Integrated Fungal Research, Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695 icarbon@ncsu.edu.
authors.forename: Ignazio
authors.initials: I
authors.lastname: Carbone
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D049770:Genes, Mating Type, Fungal; D006239:Haplotypes; D011995:Recombination, Genetic
chemical_list: D005656:Fungal Proteins
keywords: Aspergillus niger; mating type; multilocus sequence typing; sexual reproduction