title: [miR-25 promotes cell proliferation by targeting RECK in human cervical carcinoma HeLa cells].
abstract: OBJECTIVE
  To investigate the effect of miR-25 on the proliferation of human cervical carcinoma HeLa cells and its association with reversion-inducing cysteine-rich protein with Kazal motifs (RECK).
  
  
  METHODS
  The recombinant plasmids of pcDNATM6.2-GW-pre-miR-25, pmirGLO-RECK-WT, pmirGLO-RECK-MT and anti-miR-25 were constructed, and their transfection efficiencies into HeLa cells were identified by real-time quantitative PCR (qRT-PCR). The potential proliferation-stimulating function of miR-25 was analyzed by MTT assay in HeLa cells. Furthermore, the target effect of miR-25 on the RECK was determined by dual-luciferase reporter assay system, qRT-PCR and Western blotting.
  
  
  RESULTS
  Sequence analysis demonstrated that the recombinant plasmids of pcDNATM6.2-GW-pre-miR-25 and pmirGLO-RECK-WT, pmirGLO-RECK-MT were successfully constructed, and qRT-PCR revealed that the transfection efficiencies of pre-miR-25 and anti-miR-25 were desirable in HeLa cells. MTT assay showed that miR-25 over-expression promoted the proliferation of HeLa cells. In addition, the luciferase activity was significantly reduced in HeLa cells cotransfected with pre-miR-25 and RECK-WT. The qRT-PCR and Western blotting indicated that the expression level of RECK was up-regulated in HeLa cells transfected with anti-miR-25 at the transcriptional and posttranscriptional levels.
  
  
  CONCLUSIONS
  miR-25 could promote cell proliferation by targeting RECK in HeLa cells.
authors.affiliation: Second Department of Oncology, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Second Department of Oncology, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Baoshuan
authors.initials: B
authors.lastname: Fang
authors.affiliation: Third Department of Oncology, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Guohong
authors.initials: G
authors.lastname: Xin
authors.affiliation: Depatment of Nuclear Medicine, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Second Department of Geriatrics, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Xiaoye
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Depatment of Nuclear Medicine, Hebei General Hospital, Shijiazhuang 050051, China.
authors.forename: Dayong
authors.initials: D
authors.lastname: Wu
mesh_terms: D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
chemical_list: D058851:GPI-Linked Proteins; C543351:MIRN25 microRNA, human; D035683:MicroRNAs; C115460:RECK protein, human