title: Water soluble polysaccharides from Spirulina platensis: extraction and in vitro anti-cancer activity.
abstract: Polysaccharides from Spirulina platensis algae (SP) were extracted by ultrasound-assisted extraction procedure. The optimal conditions for ultrasonic extraction of SP were determined by response surface methodology. The four parameters were, extraction time (X1), extraction temperature (X2), ultrasonic power (X3) and the ratio of water to raw material (X4), respectively. The experimental data obtained were fitted to a second-order polynomial equation. The optimum conditions were extraction time of 25 min, extraction temperature 85°C, ultrasonic power 90 W and ratio of water to raw material 20 mL/g. Under these optimal conditions, the experimental yield was 13.583±0.51%, well matched with the predicted models with the coefficients of determination (R2) of 0.9971. Then, we demonstrated that SP polysaccharides had strong scavenging activities in vitro on DPPH and hydroxyl radicals. Overall, SP may have potential applications in the medical and food industries.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran. Electronic address: forouzan_kord@yahoo.com.
authors.forename: Forouzan
authors.initials: F
authors.lastname: Kurd
authors.affiliation: Department of Food Science & Technology, Ramin Agricultural & Natural Resources University, Ahvaz, Iran. Electronic address: vsamavati@ut.ac.ir.
authors.forename: Vahid
authors.initials: V
authors.lastname: Samavati
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D005591:Chemical Fractionation / Q000379:methods; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D008962:Models, Theoretical; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology; D053545:Spirulina / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D016166:Free Radical Scavengers; D011135:Polysaccharides, Bacterial
keywords: Optimization; Polysaccharides extraction; Response surface methodology (RSM); Spirulina platensis