title: A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well.
abstract: This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
authors.affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. caroline.mille.etu@univ-lemans.fr.
authors.forename: Caroline
authors.initials: C
authors.lastname: Mille
authors.affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. dominique.debarnot@univ-lemans.fr.
authors.forename: Dominique
authors.initials: D
authors.lastname: Debarnot
authors.affiliation: Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, n°1 avenue de l'Hôpital, 4000 Liège, Belgium. willy.zorzi@ulg.ac.be.
authors.forename: Willy
authors.initials: W
authors.lastname: Zorzi
authors.affiliation: Centre de Recherche sur les Protéines Prion, Institut de Pharmacie, B36, n°1 avenue de l'Hôpital, 4000 Liège, Belgium. b.elmoualij@ulg.ac.be.
authors.forename: Benaissa El
authors.initials: BE
authors.lastname: Moualij
authors.affiliation: Centre Mémoire de Ressources et Recherche, Laboratoire des Maladies à Prions, Groupement Hospitalier Est; Hôpitaux de Lyon 59 bd Pinel, 69677 Bron cedex, France CTTM, 20 rue Thalès de Milet 72000 Le Mans, France. isabelle.quadrio@chu-lyon.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Quadrio
authors.affiliation: Centre Mémoire de Ressources et Recherche, Laboratoire des Maladies à Prions, Groupement Hospitalier Est; Hôpitaux de Lyon 59 bd Pinel, 69677 Bron cedex, France CTTM, 20 rue Thalès de Milet 72000 Le Mans, France.
authors.forename: Armand
authors.initials: A
authors.lastname: Perret-Liaudet
authors.affiliation: CTTM, 20 rue Thalès de Milet 72000 Le Mans, France. acoudreuse@cttm-lemans.com.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Coudreuse
authors.affiliation: CTTM, 20 rue Thalès de Milet 72000 Le Mans, France. gilbert.legeay@neuf.fr.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Legeay
authors.affiliation: Université, UMR Université du Maine, CNRS n°6283, Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, av. O. Messiaen, 72085 Le Mans, France. fabienne.poncin-epaillard@univ-lemans.fr.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Poncin-Epaillard