title: Association between periodontal condition and subgingival microbiota in women during pregnancy: a longitudinal study.
abstract: OBJECTIVE
  In this study, the gingival conditions and the quantitative detection for Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in pregnant women were determined.
  
  
  METHODS
  Quantitative determinations of periodontal bacteria by using a SyBr green system in women during pregnancy were performed. Women at the 2nd and 3rd trimesters of pregnancy and non-pregnant women were included in this study. A. actinomycetemcomitans was observed in high numbers in women at the 2nd and 3rd trimesters of pregnancy with a significant difference (p<0.05). F. nucleatum and P. intermedia were also observed in high levels.
  
  
  CONCLUSIONS
  Our results show that pregnant women are more susceptible to gingivitis, and the presence of A. actinomycetemcomitans in subgingival biofilm might be taken into account for the treatment of periodontal disease.
authors.affiliation: Dentistry Clinic, Federal University of Espírito Santo, Vitória, ES, Brazil.
authors.forename: Priscila Viola
authors.initials: PV
authors.lastname: Borgo
authors.affiliation: Anaerobe Laboratory, Department of Microbiology, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Viviane Aparecida Arenas
authors.initials: VA
authors.lastname: Rodrigues
authors.affiliation: Dentistry Clinic, Federal University of Espírito Santo, Vitória, ES, Brazil.
authors.forename: Alfredo Carlos Rodrigues
authors.initials: AC
authors.lastname: Feitosa
authors.affiliation: Dentistry Clinic, Federal University of Espírito Santo, Vitória, ES, Brazil.
authors.forename: Karla Correa Barcelos
authors.initials: KC
authors.lastname: Xavier
authors.affiliation: Anaerobe Laboratory, Department of Microbiology, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Mario Julio
authors.initials: MJ
authors.lastname: Avila-Campos
mesh_terms: D000293:Adolescent; D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification*; D058491:Bacterial Load; D018441:Biofilms / Q000254:growth & development; D005260:Female; D016967:Fusobacterium nucleatum / Q000302:isolation & purification*; D005881:Gingiva / Q000382:microbiology*; D006801:Humans; D008137:Longitudinal Studies; D010510:Periodontal Diseases / Q000382:microbiology; D010512:Periodontal Index; D010519:Periodontium / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D018713:Prevotella intermedia / Q000302:isolation & purification*; D055815:Young Adult