title: Rab27b is a potential predictor for metastasis and prognosis in colorectal cancer.
abstract: Objective. Rab27b is reported to correlate with cancer development and progression. However, the association between Rab27b expression and the clinical characteristics of colorectal cancer (CRC) is barely investigated. Methods. One-step quantitative reverse transcription-polymerase chain reaction (qPCR) test with 18 fresh-frozen CRC samples and immunohistochemistry (IHC) analysis in 113 CRC cases were performed to explore the relationship between Rab27b expression and the clinicopathological features of CRC. Cox regression and Kaplan-Meier survival analyses were executed to evaluate the prognosis of CRC. Results. The results demonstrated that the expression levels of Rab27b mRNA and protein were significantly higher in CRC tissues than that in matched noncancerous tissues (P < 0.05). Rab27b protein expression in CRC was statistically correlated with serum CEA level (P = 0.004), lymph node metastasis (P = 0.001), distant metastasis (P = 0.009), and TNM stage (P = 0.001). Cox multifactor analysis and Kaplan-Meier method suggested that higher Rab27b protein expression (P = 0.041) and tumor differentiation (P = 0.001) were significantly associated with the overall survival of CRC patients. Conclusions. The data indicated that higher expression of Rab27b was observed in CRC tissues and Rab27b may be identified as a useful predictor of metastasis and prognosis for CRC.
authors.affiliation: Department of Gastroenterology, Jiangsu Province Geriatric Hospital, Nanjing 210000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Bao
authors.affiliation: Department of Traumatic Surgery, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China.
authors.forename: Yijiang
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Intensive Care Medicine, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Pathology, Jiangsu Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing 210000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Anesthesiology, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ge
authors.affiliation: Department of Hepatobiliary and Laparoscopic Surgery, Yixing People's Hospital, The Affiliated Yixing Hospital of Jiangsu University, Yixing 214200, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhan
authors.affiliation: Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226000, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Pathology, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China.
authors.forename: Jiabi
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The Key Laboratory of Cancer Biomarkers, Prevention & Treatment Cancer Center, and The Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210000, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Gastrointestinal Surgery, Changzhou No. 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou 213000, China.
authors.forename: Liming
authors.initials: L
authors.lastname: Tang