title: Root canal disinfection of immature dog teeth with apical periodontitis: Comparison of three different protocols.
abstract: OBJECTIVE
  The present in vivo study was designed to assess the efficacy of 3 root canal disinfection protocols in immature dog teeth with apical periodontitis (AP).
  
  
  METHODS
  Forty immature premolars with pulp necrosis and AP of five Beagle dogs were used. Three experimental disinfection protocols were established. After irrigation with 40 ml 5.25% sodium hypochlorite using the Endovac system, in Group 1 canals were flushed with QMix solution; in Group 2, canals were flushed with QMix solution and 2% chlorhexidine gel dressing was placed for two weeks; and in Group 3, triantibiotic paste dressing was placed for two weeks. Canals were sampled after periapical lesions were radiographically visible (S1), after the first disinfection session (S2) and, in groups 2 and 3, after dressing (S3).
  
  
  RESULTS
  After the first session of the disinfection protocol (S2), there was significant (p < 0.05) bacterial reduction in the three experimental groups. Microorganisms were absent in 100% of S2 samples in groups 1 and 2, and in 75% of group 3 (p > 0.05). After dressing, 87.5% of the S3 samples showed increased bacterial count: in group 2, CFU counts (median = 891) were significantly higher than in group 3 (median = 18) (p = 0.03).
  
  
  CONCLUSIONS
  In immature dog teeth with AP, root canal irrigation using QMix solution, with or without chlorhexidine gel dressing, or a triantibiotic paste dressing, provides the same level of disinfection than irrigation with 5.25% sodium hypochlorite alone in only one session. Key words:Apical periodontitis, chlorhexidine, Endovac, immature teeth, QMix solution, root canal disinfection, triantibiotic paste.
authors.affiliation: Department of Stomatology (Endodontic Section), School of Dentistry, University of Sevilla, C/ Avicena s/n, 41009-Sevilla, Spain.
authors.forename: Soledad
authors.initials: S
authors.lastname: Rodríguez-Benítez
authors.affiliation: Department of Stomatology (Endodontic Section), School of Dentistry, University of Sevilla, C/ Avicena s/n, 41009-Sevilla, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Stambolsky Guelfand
authors.affiliation: Department of Stomatology (Endodontic Section), School of Dentistry, University of Sevilla, C/ Avicena s/n, 41009-Sevilla, Spain.
authors.forename: Milagros
authors.initials: M
authors.lastname: Martín-Jiménez
authors.affiliation: Department of Stomatology (Endodontic Section), School of Dentistry, University of Sevilla, C/ Avicena s/n, 41009-Sevilla, Spain.
authors.forename: Juan-José
authors.initials: JJ
authors.lastname: Segura-Egea