title: Annexin A2 is involved in the production of classical swine fever virus infectious particles.
abstract: Annexin A2 (ANXA2) is an important host factor regulating several key processes in many viruses. To evaluate the potential involvement of ANXA2 in the life cycle of classical swine fever virus (CSFV), an RNA interference (RNAi) approach was utilized. Knockdown of ANXA2 did not impair CSFV RNA replication but significantly reduced CSFV production. A comparable reduction of extracellular and intracellular infectivity levels was detected, indicating that ANXA2 might play a role in CSFV assembly rather than in genome replication and virion release. Furthermore, ANXA2 was found to bind CSFV NS5A, an essential replicase component. Amino acids R338, N359, G378 of NS5A were revealed to be pivotal for the ANXA2-NS5A interaction. Substitutions of these amino acids had no effect on viral RNA replication but substantially reduced CSFV production, which might partly be due to these mutations destroying the ANXA2-NS5A interaction. These results suggested that ANXA2 might participate in CSFV production process by binding NS5A.
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Chun
authors.initials: C
authors.lastname: Sheng
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Xiaoxiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Qiuyue
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Chenhao
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.affiliation: College of Life and Environment Sciences, Shanghai Normal University, Shanghai, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Xiao
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000502:physiology*; D004252:DNA Mutational Analysis; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D011485:Protein Binding; D025941:Protein Interaction Mapping; D034622:RNA Interference; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D019065:Virus Assembly; D057074:Virus Release; D014779:Virus Replication
chemical_list: D017306:Annexin A2; D017361:Viral Nonstructural Proteins