title: Protective effect of extracts of Baliospermum montanum (Willd.) Muell.-Arg. against paracetamol-induced hepatotoxicity-an in vivo and in vitro study.
abstract: OBJECTIVE
  Evaluation of hepatoprotective effect of ethyl methyl ketone and methanol sub-fractions obtained from methanol fraction of total methanol extract (ME) was carried out both in vivo and in vitro using paracetamol-induced toxicity.
  
  
  METHODS
  Hepatoprotective activity in vivo was assessed by determining the serum levels of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), alkaline phosphatase, total bilirubin, total cholesterol, total protein (TPTN), and albumin in serum. The studies were supported by histopathological examination of liver sections. In vitro activity was assessed by determining the change in hepatocyte viability and other parameters such as GOT, GPT and TPTN.
  
  
  METHODS
  The ME of the roots of Baliospermum montanum was prepared and fractionated with chloroform and methanol to get activity guided fraction. The bio-active guided methanol fraction was further fractionated with ethyl methyl ketone and methanol. Both the fractions were evaluated for hepatoprotective activity against paracetamol-induced toxicity.
  
  
  METHODS
  For determining the significant intergroup difference each parameter was analyzed separately, and one-way analysis of variance was carried out and the individual comparisons of the group mean values were done using Dunnet's test.
  
  
  RESULTS
  Methanol sub-fraction prevented hepatic damage in vivo, induced by paracetamol, whereas both the sub-fractions showed hepatoprotective effect by restoring altered parameters in the selected in vitro model. The results were comparable with the standard hepatoprotective drug silymarin.
  
  
  CONCLUSIONS
  This study underlines the therapeutic potential of B. montanum as per claims in Ayurveda in liver disorders.
authors.affiliation: Department of Pharmacognosy and Phytochemistry, Creative Educational Society's College of Pharmacy, Chinnatekur, Kurnool, Andhra Pradesh, India.
authors.forename: S V Suresh
authors.initials: SV
authors.lastname: Kumar
authors.affiliation: Department of Pharmacy, The MS University of Baroda, Vadodara, Gujarat, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Mishra
keywords: Baliospermum montanum; methanol sub-fraction; paracetamol; silymarin