title: Antitumor effects of a dual cancer-specific oncolytic adenovirus on colorectal cancerin vitroandin vivo.
abstract: The efficacy and specificity of treatment are major challenges for cancer gene therapy. Oncolytic virotherapy is an attractive drug delivery platform for cancer gene therapy. In the present study, the dual-specific antitumor oncolytic adenovirus, Ad-Apoptin-hTERT-E1a, was used to infect SW1116 human colorectal carcinoma (CRC) cell lines and CT26 mouse-CRC-cell bearing BALB/c mouse models for testing antitumor effectsin vitroandin vivo. Thein vitroassays revealed that infection with Ad-Apoptin-hTERT-E1a induced a significant cytotoxic effect on the CRC cell line, SW1116; however, the normal human cell line, GES, was only slightly inhibited by the recombinant adenovirus. Acridine orange and ethidium bromide staining and an annexin V assay indicated that infection of SW1116 cells with Ad-Apoptin-hTERT-E1a resulted in a significant induction of apoptosis. Furthermore, western blotting and flow cytometry revealed a decrease in the mitochondrial membrane potential (MMP), the release of cytochromecand the activation of caspase 3, 6 and 7 in Ad-Apoptin-hTERT-E1a-infected SW1116 cells. In the animal models, Ad-Apoptin-hTERT-E1a was shown to significantly inhibit tumor growth and extend the survival times of the animals. Therefore, the experimental results indicated that Ad-Apoptin-hTERT-E1a has potential for application in tumor gene therapy.
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China ; Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Jilin Province Qianwei Hospital, Changchun, Jilin 130031, P.R. China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Head and Neck Surgery, Jilin Province Tumor Hospital, Changchun, Jilin 130001, P.R. China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Hu
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sheng
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Jilin Province Qianwei Hospital, Changchun, Jilin 130031, P.R. China.
authors.forename: Dexing
authors.initials: D
authors.lastname: Chen
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin 130122, P.R. China ; Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, Jilin 130021, P.R. China.
authors.forename: Ningyi
authors.initials: N
authors.lastname: Jin
keywords: GES cell line; SW1116 cell line; apoptin; colorectal carcinoma; oncolytic adenovirus