title: RBPJ inhibition impairs the growth of lung cancer.
abstract: The exact effects of the modulation of Notch signaling pathway on cell growth have been shown to depend on tumor cell type. Recombination signal-binding protein Jκ (RBPJ) is a key transcription factor downstream of receptor activation in Notch signaling pathway. Here, we evaluated the effects of RBPJ inhibition on the growth of lung cancer cells. We found that a short hairpin interfering RNA (shRNA) for RBPJ efficiently inhibited RBPJ expression in lung cancer cells, resulting in a significant decrease in the cell growth. Further analyses showed that RBPJ inhibition altered the levels of its downstream targets, including p21, p27, CDK2, Hes1, Bcl-2, and SKP2, to prevent the cells from growing. Our data thus suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human lung cancer.
authors.affiliation: Department of Pulmonary Medicine, The Affiliated Hospital of Hangzhou Normal University, 126 Wenzhou Road, 310015, Hangzhou, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Lv
authors.forename: Ronglin
authors.initials: R
authors.lastname: Shen
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
mesh_terms: D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D058990:Molecular Targeted Therapy; D009363:Neoplasm Proteins / Q000096:biosynthesis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D034622:RNA Interference; D051880:Receptors, Notch / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics
chemical_list: D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D009363:Neoplasm Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C497570:RBPJ protein, human; D051880:Receptors, Notch