title: Molecular study of Trypanosoma caninum isolates based on different genetic markers.
abstract: Trypanosoma caninum is a parasite recently described in dogs, whose life cycle is rather unknown. Here, we performed a genetic study with T. caninum samples obtained in different Brazilian regions. The study was based on PCR assays target to small and large subunit ribosomal DNA (rDNA) (18S rDNA and 24Sα rDNA), cytochrome B (Cyt b), and internal transcribed spacer 1 rDNA (ITS1 rDNA) following by the sequence analysis. Additionally, we used primers for the variable regions of kinetoplast DNA (kDNA) minicircles and endonucleases restriction in the ITS1 rDNA amplification product. T. caninum samples displayed the same patterns. Tree construction confirmed the close relationship between T. caninum samples, regardless of the molecular target used and endonuclease restriction digestion revealed that all samples have the same restriction profile. Therefore, T. caninum seems to be a genetically homogeneous specie. In the kDNA assay, T. caninum possessed a different molecular size profile with respect to others trypanosomes, 330 and 350 bp. This study provides nucleotide sequences from different regions of the genome of T. caninum that certainly facilitate future studies.
authors.affiliation: Programa de Pós-Graduação em Pesquisa Clínica em Doenças Infecciosas, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil 4365, Rio de Janeiro, RJ, 21040-900, Brazil.
authors.forename: Juliana H S
authors.initials: JH
authors.lastname: Barros
authors.forename: Helena K
authors.initials: HK
authors.lastname: Toma
authors.forename: Maria
authors.initials: M
authors.lastname: de Fatima Madeira
mesh_terms: D000818:Animals; D001483:Base Sequence; D001938:Brazil; D045303:Cytochromes b / Q000235:genetics; D017931:DNA Primers; D018105:DNA, Kinetoplast / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D005819:Genetic Markers; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014345:Trypanosoma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014352:Trypanosomiasis / Q000469:parasitology / Q000662:veterinary
chemical_list: D017931:DNA Primers; D018105:DNA, Kinetoplast; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D021903:DNA, Ribosomal Spacer; D005819:Genetic Markers; D012337:RNA, Ribosomal, 18S; D045303:Cytochromes b