title: Requirement for Fc effector mechanisms in the APOBEC3/Rfv3-dependent neutralizing antibody response.
abstract: Antiretroviral neutralizing antibody (NAb) responses are often evaluated in the absence of Fc-dependent immune effectors. In murine Friend retrovirus infection, Apobec3/Rfv3 promotes a potent polyclonal NAb response. Here, we show that the Apobec3/Rfv3-dependent NAb response correlated with virus-specific IgG2 titers and that the in vivo neutralization potency of Apobec3/Rfv3-resistant antisera was dependent on activating Fcγ receptors but not complement. The data strengthen retroviral vaccine strategies aimed at eliciting NAbs that activate specific Fcγ receptors.
authors.affiliation: Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA Department of Immunology and Microbiology, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: Kalani
authors.initials: K
authors.lastname: Halemano
authors.affiliation: Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: Bradley S
authors.initials: BS
authors.lastname: Barrett
authors.affiliation: Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: Karl J
authors.initials: KJ
authors.lastname: Heilman
authors.affiliation: Department of Immunology and Microbiology, University of Colorado Denver, Aurora, Colorado, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Morrison
authors.affiliation: Department of Medicine, University of Colorado Denver, Aurora, Colorado, USA Department of Immunology and Microbiology, University of Colorado Denver, Aurora, Colorado, USA mario.santiago@ucdenver.edu.
authors.forename: Mario L
authors.initials: ML
authors.lastname: Santiago
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D003564:Cytidine Deaminase / Q000378:metabolism*; D005622:Friend murine leukemia virus / Q000276:immunology*; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007074:Immunoglobulin G / Q000276:immunology; D008810:Mice, Inbred C57BL
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; C503504:Apobec3 protein, mouse; D003564:Cytidine Deaminase