title: [The associations of ulcerative colitis with vascular endothelial growth factor (-2578C/A) and (+936C/T) single nucleotide polymorphisms].
abstract: OBJECTIVE
  To investigate the association of (-2578C/A) and (+936C/T) single nucleotide polymorphism(SNPs) of vascular endothelial growth factor (VEGF) gene with the susceptibility to ulcerative colitis (UC).
  
  
  METHODS
  A total of 373 UC patients and 503 healthy controls were recruited. The (-2578C/A) and (+936C/T) polymorphism of VEGF gene were detected using a mini-sequencing technique.
  
  
  RESULTS
  By an unconditional logistic regression analysis, the frequencies of the mutant allele T and genotype CT+TT of VEGF gene (+936C/T) were significantly decreased in patients with severe UC compared to the controls (10.4% vs 19.3%, OR = 0.487, 95%CI 0.248-0.954, P = 0.036; 18.8% vs 33.8%, OR = 0.452, 95%CI 0.214-0.955, P = 0.037, respectively). Moreover, patients with severe UC had significant lower rates of mutant allele T and genotype CT+TT compared with patients with mild and moderate UC (10.4% vs 20.5%, OR = 0.452, 95%CI 0.229-0.894, P = 0.022; 18.8% vs 36.9%, OR = 0.394, 95%CI 0.185-0.842, P = 0.016, respectively). The frequencies of mutant allele A and genotype CA+AA of VEGF (-2578C/A) gene were not statistically different between UC patients and the controls. Moreover, they were not significantly associated with the clinicopathologic features in UC patients.
  
  
  CONCLUSIONS
  The mutation of VEGF (+936C/T) gene is correlated with the severity of UC. However, the polymorphism of VEGF (-2578C/A) gene is not significantly related to the susceptibility to UC.
authors.forename: Liqin
authors.initials: L
authors.lastname: Yu
authors.forename: Shenglong
authors.initials: S
authors.lastname: Xia
authors.forename: Bo
authors.initials: B
authors.lastname: Zheng
authors.forename: Maodong
authors.initials: M
authors.lastname: Guo
authors.forename: Xinxin
authors.initials: X
authors.lastname: Lin
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Lin
authors.forename: Lijia
authors.initials: L
authors.lastname: Jiang
authors.forename: Ran
authors.initials: R
authors.lastname: Ding
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Email: wzjiangyi@yeah.net.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000483:Alleles; D003093:Colitis, Ulcerative / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D009154:Mutation; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D042461:Vascular Endothelial Growth Factor A