title: Ferrocene-functionalized 4-(2,5-Di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline: a novel design in conducting polymer-based electrochemical biosensors.
abstract: Herein, we report a novel ferrocenyldithiophosphonate functional conducting polymer and its use as an immobilization matrix in amperometric biosensor applications. Initially, 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)amidoferrocenyldithiophosphonate was synthesized and copolymerized with 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine at graphite electrodes. The amino groups on the polymer were utilized for covalent attachment of the enzyme glucose oxidase. Besides, ferrocene on the backbone was used as a redox mediator during the electrochemical measurements. Prior to the analytical characterization, optimization studies were carried out. The changes in current signals at +0.45 V were proportional to glucose concentration from 0.5 to 5.0 mM. Finally, the resulting biosensor was applied for glucose analysis in real samples and the data were compared with the spectrophotometric Trinder method.
authors.affiliation: Chemistry Department, Faculty of Art and Science, Pamukkale University, 20070 Denizli, Turkey. rukiye.yagiz@gmail.com.
authors.forename: Rukiye
authors.initials: R
authors.lastname: Ayranci
authors.affiliation: Biochemistry Department, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey. dilek.odaci.demirkol@ege.edu.tr.
authors.forename: Dilek Odaci
authors.initials: DO
authors.lastname: Demirkol
authors.affiliation: Chemistry Department, Faculty of Art and Science, Pamukkale University, 20070 Denizli, Turkey. metinak@pau.edu.tr.
authors.forename: Metin
authors.initials: M
authors.lastname: Ak
authors.affiliation: Biochemistry Department, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey. suna.timur@ege.edu.tr.
authors.forename: Suna
authors.initials: S
authors.lastname: Timur
mesh_terms: D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D005947:Glucose / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D000075163:Metallocenes; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013876:Thiophenes / Q000138:chemical synthesis / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry
chemical_list: C000600614:4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)aniline; D000814:Aniline Compounds; D004800:Enzymes, Immobilized; D005296:Ferrous Compounds; D000075163:Metallocenes; D011108:Polymers; D011758:Pyrroles; D013876:Thiophenes; D017971:Tin Compounds; C109984:indium tin oxide; D005947:Glucose; C004998:ferrocene