title: Differentiated embryonic chondrocytes 1 expression of periodontal ligament tissue and gingival tissue in the patients with chronic periodontitis.
abstract: OBJECTIVE
  To evaluate the DEC1 expression of periodontal ligament tissue and gingival tissue in the patients with chronic periodontitis.
  
  
  METHODS
  20 non-smoking patients with chronic periodontitis and 20 healthy individuals were enrolled. Periodontal ligament tissue and gingival tissue samples from healthy subjects were collected during teeth extraction for orthodontic reason or the third molar extraction. The parallel samples from patients with chronic periodontitis were obtained during periodontal flap operations or teeth extraction as part of periodontal treatment. The DEC1 expression and the alkaline phosphatase (ALP) activity of both the periodontal ligament tissue and gingival tissue were determined by Western blot, Immunohistochemistry and ALP Detection Kit.
  
  
  RESULTS
  The DEC1 expression of periodontal ligament tissue in the patients with chronic periodontitis decreased significantly along with the decreased ALP activity. On the contrary, the DEC1 expression of gingival tissue in the patients with chronic periodontitis increased significantly. Further study found that the DEC1 expression of gingival tissue increased mainly in the suprabasal layer of gingival epithelial cells but decreased in the gingival connective tissue of the patients with chronic periodontitis.
  
  
  CONCLUSIONS
  The DEC1 expression decreases in the periodontal ligament tissue which is related to the osteogenic capacity, whereas the DEC1 expression increases in the suprabasal layer of gingival epithelial cells which are involved in immune inflammatory response in the patients with chronic periodontitis. The findings provide a new target to explore the pathology and the therapy of periodontitis.
authors.affiliation: Department of Stomatology, Jinling Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Shenlin
authors.initials: S
authors.lastname: Hu
authors.affiliation: Department of Pharmacology, Nanjing Medicine University, Nanjing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shang
authors.affiliation: Department of Pharmacology, Nanjing Medicine University, Nanjing, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Yue
authors.affiliation: Department of Pharmacology, Nanjing Medicine University, Nanjing, China.
authors.forename: Ruini
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Stomatology, Jinling Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Pharmacology, Nanjing Medicine University, Nanjing, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Stomatology, Jinling Hospital, Nanjing Medical University, Nanjing, China. Electronic address: maozhao402@163.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Mao
authors.affiliation: Department of Pharmacology, Nanjing Medicine University, Nanjing, China. Electronic address: jianyang@njmu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D015153:Blotting, Western; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000601:surgery; D005260:Female; D005881:Gingiva / Q000166:cytology / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D013524:Surgical Flaps
chemical_list: D000469:Alkaline Phosphatase; D051792:Basic Helix-Loop-Helix Proteins; D018398:Homeodomain Proteins; C107989:BHLHE40 protein, human
keywords: Chronic periodontitis; Differentiated embryonic chondrocytes 1 (DEC1); Gingival epithelial cells; Gingival tissue; Periodontal ligament tissue