title: Inhibitory constituents of Sophora tonkinensis on nitric oxide production in RAW 264.7 macrophages.
abstract: Bioactivity-guided fractionation of the MeOH extract from the roots of Sophora tonkinensis resulted in the isolation of a new pterocarpan glycoside (1) together with nine known compounds, maackiain (2), sophoranone (3), sophoranochromene (4), pinoresinol (5), syringaresinol (6), medioresinol (7), 4',7-dihydroxyflavone (8), calycosin (9), and genistein (10). The structure of the new compound was determined on the basis of spectroscopic analysis including NMR and CD data in combination with acid hydrolysis. Compounds 1-4 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 13.6 to 33.0μM in RAW 264.7 macrophages.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea; Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeonbuk 580-185, Republic of Korea.
authors.forename: Chul
authors.initials: C
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Qinghao
authors.initials: Q
authors.lastname: Jin
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 362-763, Republic of Korea. Electronic address: byhwang@chungbuk.ac.kr.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D002460:Cell Line; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D029909:Sophora / Q000737:chemistry*
chemical_list: D009569:Nitric Oxide
keywords: Leguminosae; Nitric oxide production inhibitor; Pterocarpan; Sophora tonkinensis