title: [Inhibition of sunitinib on the expressions of PD-L1 and PD-L2 of mouse bone marrow-derived dendritic cells].
abstract: OBJECTIVE
  To observe the changes of programmed death-1 ligand 1 (PD-L1) and PD-L2 expressions on mouse bone marrow-derived dendritic cells (DCs) stimulated by sunitinib.
  
  
  METHODS
  DCs were randomly divided into four groups which were treated with sunitinib (100, 200, 300 ng/mL) and dimethylsulfoxide (DMSO), respectively. After 48 hours, PD-L1 and PD-L2 expression levels were analyzed by flow cytometry.
  
  
  RESULTS
  Compared with the control group, the expression of PD-L1 on mature DCs (mDCs) and all DCs [including mature DCs and immature DCs (imDCs)] was significantly down-regulated in sunitinib treatment groups. The PD-L1 expression percentages of imDCs, mDCs and DCs were significantly reduced in sunitinib treatment groups; the percentage of mDCs expressing PD-L2 also dropped in all treatment groups, and the percentage of DCs expressing PD-L2 decreased in 100 and 300 ng/mL sunitinib treatment groups.
  
  
  CONCLUSIONS
  Sunitinib can significantly reduce the expressions of PD-L1 and PD-L2 on mouse DCs.
authors.affiliation: Department of Urology, Clinical College, China PLA 307 Hospital, Anhui Medical University, Beijing 100071, China.
authors.forename: Feilong
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Haixing
authors.initials: H
authors.lastname: Mai
authors.affiliation: Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Jinkai
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xuechao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Urology, Clinical College, China PLA 307 Hospital, Anhui Medical University, Department of Urology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D060890:B7-H1 Antigen / Q000235:genetics* / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D007211:Indoles / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000235:genetics* / Q000378:metabolism; D011758:Pyrroles / Q000494:pharmacology*; D000077210:Sunitinib
chemical_list: D000970:Antineoplastic Agents; D060890:B7-H1 Antigen; C498919:Cd274 protein, mouse; D007211:Indoles; C556978:Pdcd1lg2 protein, mouse; D061027:Programmed Cell Death 1 Ligand 2 Protein; D011758:Pyrroles; D000077210:Sunitinib