title: Cytotoxicity of resin composites containing bioactive glass fillers.
abstract: OBJECTIVE
  To determine the in vitro cytotoxicity of dental composites containing bioactive glass fillers.
  
  
  METHODS
  Dental composites (50:50 Bis-GMA/TEGDMA resin: 72.5wt% filler, 67.5%Sr-glass and 5% OX50) containing different concentrations (0, 5, 10 and 15wt%) of two sol-gel bioactive glasses, BAG65 (65mole% SiO2, 31mole% CaO, 4mole% P2O5) and BAG61 (3mole% F added) were evaluated for cytotoxicity using Alamar Blue assay. First, composite extracts were obtained from 7 day incubations of composites in cell culture medium at 37°C. Undifferentiated pulp cells (OD-21) were exposed to dilutions of the original extracts for 3, 5, and 7 days. Then freshly cured composite disks were incubated with OD-21 cells (n=5) for 2 days. Subsequently, fresh composite disks were incubated in culture medium at 37°C for 7 days, and then the extracted disks were incubated with OD-21 cells for 2 days. Finally, fresh composites disks were light cured for 3, 5, and 20s and incubated with OD-21 cells (n=5) for 1, 3, 5, and 7 days. To verify that the three different curing modes produced different levels of degree of conversion (DC), the DC of each composite was determined by FTIR. Groups (n=5) were compared with ANOVA/Tukey's (α≤0.05).
  
  
  RESULTS
  Extracts from all composites significantly reduced cell viability until a dilution of 1:8 or lower, where the extract became equal to the control. All freshly-cured composites showed significantly reduced cell viability at two days. However, no reduction in cell viability was observed for any composite that had been previously soaked in media before exposure to the cells. Composites with reduced DC (3s vs. 20s cure), as verified by FTIR, showed significantly reduced cell viability.
  
  
  CONCLUSIONS
  The results show that the composites, independent of composition, had equivalent potency in terms of reducing the viability of the cells in culture. Soaking the composites for 7 days before exposing them to the cells suggested that the "toxic" components had been extracted and the materials were no longer cytotoxic. The results demonstrate that the cytotoxicity of composites with and without BAG must predominantly be attributed to the release of residual monomers, and not to the presence of the BAG.
authors.affiliation: Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA. Electronic address: salehi@ohsu.edu.
authors.forename: Satin
authors.initials: S
authors.lastname: Salehi
authors.affiliation: Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Gwinner
authors.affiliation: Midwestern University, Glendale, AZ, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Mitchell
authors.affiliation: Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA.
authors.forename: Carmem
authors.initials: C
authors.lastname: Pfeifer
authors.affiliation: Division of Biomaterials and Biomechanics, Department of Restorative, School of Dentistry, Oregon Health Science University, Portland, OR, USA.
authors.forename: Jack L
authors.initials: JL
authors.lastname: Ferracane
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003188:Composite Resins / Q000633:toxicity*; D003764:Dental Materials / Q000633:toxicity; D003782:Dental Pulp / Q000166:cytology*; D005898:Glass; D066298:In Vitro Techniques; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000633:toxicity; D011109:Polymethacrylic Acids / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D003188:Composite Resins; D003764:Dental Materials; D011109:Polymethacrylic Acids; C020946:triethylene glycol dimethacrylate; D011092:Polyethylene Glycols; D017438:Bisphenol A-Glycidyl Methacrylate
keywords: Bioactive glass; Cytotoxicity; Degree of Conversion; Dental composites; FTIR; Undifferentiated pulp cells (OD-21)