title: [Blood sampling using "dried blood spot": a clinical biology revolution underway?].
abstract: Blood testing using the dried blood spot (DBS) is used since the 1960s in clinical analysis, mainly within the framework of the neonatal screening (Guthrie test). Since then numerous analytes such as nucleic acids, small molecules or lipids, were successfully measured on the DBS. While this pre-analytical method represents an interesting alternative to classic blood sampling, its use in routine is still limited. We review here the different clinical applications of the blood sampling on DBS and estimate its future place, supported by the new methods of analysis as the LC-MS mass spectrometry.
authors.affiliation: Institut de recherche en biothérapie, Hôpital Saint Eloi, CHU Montpellier, France ; Laboratoire de biochimie clinique de la protéomique et CCBHM, Université Montpellier 1, Montpellier, France ; Inserm, F-34000 U1040, Montpellier, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Hirtz
authors.affiliation: Institut de recherche en biothérapie, Hôpital Saint Eloi, CHU Montpellier, France ; Laboratoire de biochimie clinique de la protéomique et CCBHM, Université Montpellier 1, Montpellier, France ; Inserm, F-34000 U1040, Montpellier, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Lehmann
mesh_terms: D001798:Blood Proteins / Q000032:analysis; D001800:Blood Specimen Collection / Q000379:methods* / Q000639:trends*; D002241:Carbohydrates / Q000032:analysis / Q000097:blood; D059788:Dried Blood Spot Testing / Q000379:methods / Q000639:trends; D023281:Genomics / Q000379:methods; D006801:Humans; D008055:Lipids / Q000032:analysis / Q000097:blood; D009696:Nucleic Acids / Q000032:analysis / Q000302:isolation & purification; D010455:Peptides / Q000032:analysis / Q000097:blood; D015262:Xenobiotics / Q000032:analysis / Q000097:blood
chemical_list: D001798:Blood Proteins; D002241:Carbohydrates; D008055:Lipids; D009696:Nucleic Acids; D010455:Peptides; D015262:Xenobiotics
keywords: ELISA; PCR; dried blood spot; mass spectrometry; multiplex; pre-analytical