title: PSTK is a novel gene associated with early lung injury in Paraquat Poisoning.
abstract: OBJECTIVE
  Paraquat Poisoning (PQ) can cause illness and death, and its main causes of mortality are acute respiratory failure and lung fibrosis. Early recognition of this condition and early treatment are vital. Thus, it is of importance to target the key genes controlling pathogenesis in the early stage of PQ.
  
  
  METHODS
  C57BL/6 mice were used for Paraquat intragastric administration as a model of PQ. Following a gene chip-based screening, the change of gene expression in the lung was further validated by bioinformatic analyses, co-expression network construction and real-time RT-PCR, Western blot and immunofluorescence assays.
  
  
  RESULTS
  2287 genes with differential expression were identified at the very early stage of PQ. From these, 76 genes that were linked to mitochondrion function were further pursued. Among these genes, PSTK was a phosphorylase kinase which serves a protective role in oxidative stress lung damage. PSTK was the central gene in a 30-gene network that is important for mitochondrial complex I assembly, mitochondrial apoptosis and mitochondrial fatty acid beta-oxidation, suggesting that they could conceivably be related to the pathogenesis of PQ induced lung damage. Lastly, we confirmed that PSTK was lowered in rodent lungs following PQ.
  
  
  CONCLUSIONS
  PSTK emerges as a central gene in a network of mitochondrial function genes in PQ exposed mice. The functional role of PSTK in PQ induced lung injury warrants further examination.
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Changbin
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Cang
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Yaxiang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Chandra
authors.initials: C
authors.lastname: Mohan
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. Electronic address: hdy455@hotmail.com.
authors.forename: Dayong
authors.initials: D
authors.lastname: Hu
authors.affiliation: Department of Nephrology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China. Electronic address: pengai@tongji.edu.cn.
authors.forename: Ai
authors.initials: A
authors.lastname: Peng
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D016000:Cluster Analysis; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D063990:Gene Ontology; D053263:Gene Regulatory Networks / Q000235:genetics*; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D010269:Paraquat / Q000008:administration & dosage / Q000506:poisoning*; D010764:Phosphorylase Kinase / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: C000596816:PSTK protein, mouse; D010764:Phosphorylase Kinase; D010269:Paraquat
keywords: Microarray; PSTK; Paraquat Poisoning