title: Development and Evaluation of Transgenic Nude Mice Expressing Ubiquitous Green Fluorescent Protein.
abstract: OBJECTIVE
  Researchers had developed and characterized transgenic green/red fluorescent protein (GFP/RFP) nude mouse with ubiquitous RFP or GFP expression, but none has evaluated the level of immune cells and expression levels of GFP in this model.
  
  
  METHODS
  The nude GFP mice were evaluated by imaging, hematological indices, and flow cytometry to compare the proportion of immune T cells. Quantitative real-time PCR (qRT-PCR) was done for evaluating the relative expression of GFP transcripts in few organs of the nude GFP mice.
  
  
  RESULTS
  The hematological and immune cells of nude GFP were within the range of nude mice. However, the gene expression levels were relatively less in various tissues compared with B6 GFP mice.
  
  
  CONCLUSIONS
  These findings suggest that nude GFP is an ideal model resembling normal nude mice; however, GFP expression in various tissues by fluorescence should be considered, as the expression of GFP differs in various organs.
authors.affiliation: National Institute of Immunology, New Delhi, 110067, India.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Iyer
authors.forename: Shailendra
authors.initials: S
authors.lastname: Arindkar
authors.forename: Alaknanda
authors.initials: A
authors.lastname: Mishra
authors.forename: Kapil
authors.initials: K
authors.lastname: Manglani
authors.forename: Jerald Mahesh
authors.initials: JM
authors.lastname: Kumar
authors.forename: Subeer S
authors.initials: SS
authors.lastname: Majumdar
authors.forename: Pramod
authors.initials: P
authors.lastname: Upadhyay
authors.forename: Perumal
authors.initials: P
authors.lastname: Nagarajan
mesh_terms: D000818:Animals; D005260:Female; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008822:Mice, Transgenic; D057054:Molecular Imaging / Q000379:methods*; D012107:Research Design
chemical_list: D049452:Green Fluorescent Proteins