title: Piper betleextracts exhibit antitumor activity by augmenting antioxidant potential.
abstract: The present study was conducted to evaluate the methanolic extract ofPiper betleleaves (MPBL) and its organic fractions with regard to antitumor activity against Ehrlich ascites carcinoma (EAC) in Swiss albino mice and to confirm their antioxidant activities. At 24 h post-intraperitoneal inoculation of tumor cells into mice, extracts were administered at 25, 50 and 100 mg/kg body weight for nine consecutive days. The antitumor effects of the extracts were then assessed according to tumor volume, packed cell count, viable and non-viable tumor cell count, median survival time and increase in life span of EAC-bearing mice. Next, hematological profiles and serum biochemical parameters were calculated, and antioxidant properties were assessed by estimating lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels. MPBL and the ethylacetate fraction (EPBL) at a dose of 100 mg/kg induced a significant decrease in tumor volume, packed cell volume and viable cell count and increased the life span of the EAC-bearing mice (P<0.05). Hematological and serum biochemical profiles were restored to normal levels in the extract-treated mice compared with the EAC control mice. MPBL and EPBL treatment significantly decreased lipid peroxidation (P<0.05) and restored GSH, SOD and CAT levels towards normal compared with the EAC control. Taken together, the results of the present study demonstrated thatPiper betleextracts exhibit significant antitumor activity, which may be attributed to the augmentation of endogenous antioxidant potential.
authors.affiliation: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea ; Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
authors.forename: Badrul
authors.initials: B
authors.lastname: Alam
authors.affiliation: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
authors.forename: Rajib
authors.initials: R
authors.lastname: Majumder
authors.affiliation: Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh.
authors.forename: Shahina
authors.initials: S
authors.lastname: Akter
authors.affiliation: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea ; Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Sang-Han
authors.initials: SH
authors.lastname: Lee
keywords: Ehrlich ascites carcinoma; Piper betle; antioxidant; antitumor