title: A Long Noncoding RNA on the Ribosome Is Required for Lifespan Extension.
abstract: The biogenesis of ribosomes and their coordination of protein translation consume an enormous amount of cellular energy. As such, it has been established that the inhibition of either process can extend eukaryotic lifespan. Here, we used next-generation sequencing to compare ribosome-associated RNAs from normal strains of Caenorhabditis elegans to those carrying the life-extending daf-2 mutation. We found a long noncoding RNA (lncRNA), transcribed telomeric sequence 1 (tts-1), on ribosomes of the daf-2 mutant. Depleting tts-1 in daf-2 mutants increases ribosome levels and significantly shortens their extended lifespan. We find tts-1 is also required for the longer lifespan of the mitochondrial clk-1 mutants but not the feeding-defective eat-2 mutants. In line with this, the clk-1 mutants express more tts-1 and fewer ribosomes than the eat-2 mutants. Our results suggest that the expression of tts-1 functions in different longevity pathways to reduce ribosome levels in a way that promotes life extension.
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Paul B
authors.initials: PB
authors.lastname: Essers
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Julie
authors.initials: J
authors.lastname: Nonnekens
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Yvonne J
authors.initials: YJ
authors.lastname: Goos
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Marco C
authors.initials: MC
authors.lastname: Betist
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Marjon D
authors.initials: MD
authors.lastname: Viester
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Britt
authors.initials: B
authors.lastname: Mossink
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Nico
authors.initials: N
authors.lastname: Lansu
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands.
authors.forename: Hendrik C
authors.initials: HC
authors.lastname: Korswagen
authors.affiliation: Centre of Microbial and Plant Genetics, KU Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium.
authors.forename: Rob
authors.initials: R
authors.lastname: Jelier
authors.affiliation: Section Metabolic Diseases, Department of Molecular Cancer Research, Wilhelmina Children's Hospital, University Medical Center Utrecht, 3508 AB Utrecht, the Netherlands.
authors.forename: Arjan B
authors.initials: AB
authors.lastname: Brenkman
authors.affiliation: Hubrecht Institute, KNAW and University Medical Center Utrecht, 3584 CT Utrecht, the Netherlands. Electronic address: a.macinnes@sanquin.nl.
authors.forename: Alyson W
authors.initials: AW
authors.lastname: MacInnes