title: Inhibition of abasic site cleavage in bubble DNA by multifunctional protein YB-1.
abstract: Y-box binding protein 1 (YB-1) is widely known to participate in a multiple DNA and RNA processing events in the living cell. YB-1 is also regarded as a putative component of DNA repair. This possibility is supported by relocalization of YB-1 into the nucleus following genotoxic stress. Increased affinity of YB-1 for damaged DNA, especially in its single-stranded form, and its functional interaction with proteins responsible for the initiation of apurinic/apyrimidinic (AP) site repair, namely, AP endonuclease 1 and DNA glycosylase NEIL1, suggest that YB-1 could be involved in the repair of AP sites as a regulatory protein. Here we show that YB-1 has a significant inhibitory effect on the cleavage of AP sites located in single-stranded DNA and in DNA bubble structures. Such interference may be considered as a possible mechanism to prevent single-stranded intermediates of DNA replication, transcription and repair from being converted into highly genotoxic DNA strand breaks, thus allowing the cell to coordinate different DNA processing mechanisms.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: Elizaveta E
authors.initials: EE
authors.lastname: Fomina
authors.forename: Pavel E
authors.initials: PE
authors.lastname: Pestryakov
authors.forename: Dmitry A
authors.initials: DA
authors.lastname: Kretov
authors.forename: Dmitry O
authors.initials: DO
authors.lastname: Zharkov
authors.forename: Lev P
authors.initials: LP
authors.lastname: Ovchinnikov
authors.forename: Patrick A
authors.initials: PA
authors.lastname: Curmi
authors.forename: Olga I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004249:DNA Damage; D045647:DNA Glycosylases / Q000737:chemistry* / Q000235:genetics; D004260:DNA Repair; D004261:DNA Replication; D004277:DNA, Single-Stranded; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D013379:Substrate Specificity; D051840:Y-Box-Binding Protein 1 / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D051840:Y-Box-Binding Protein 1; C070856:YBX1 protein, human; D004247:DNA; D045647:DNA Glycosylases; C473634:NEIL1 protein, human; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
keywords: AP endonuclease 1 (APE1); DNA repair; NEIL1; Y-box binding protein 1 (YB-1); abasic site (AP site)