title: Neutralization of staphylococcal enterotoxin B by an aptamer antagonist.
abstract: Staphylococcal enterotoxin B (SEB) is a major virulence factor for staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T lymphocytic population, releasing proinflammatory cytokines. Blocking SEB-initiated toxicity may be an effective strategy for treating TSS. Using a process known as systematic evolution of ligands by exponential enrichment (SELEX), we identified an aptamer that can antagonize SEB with nanomolar binding affinity (Kd = 64 nM). The aptamer antagonist effectively inhibits SEB-mediated proliferation and cytokine secretion in human peripheral blood mononuclear cells. Moreover, a PEGylated aptamer antagonist significantly reduced mortality in a "double-hit" mouse model of SEB-induced TSS, established via sensitization with d-galactosamine followed by SEB challenge. Therefore, our novel aptamer antagonist may offer potential therapeutic efficacy against SEB-mediated TSS.
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Longjie
authors.initials: L
authors.lastname: Gan
authors.affiliation: Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Xianhui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Xiangyue
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Institute of Laboratory Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, People's Republic of China Department of Laboratory Medicine, Dongfang Hospital, Xiamen University, Fuzhou, People's Republic of China Department of Laboratory Medicine, Fuzong Clinical College, Fujian Medical University, Fuzhou, People's Republic of China lanxp@sina.com.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Lan
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003001:Cloning, Molecular; D019295:Computational Biology; D016207:Cytokines / Q000096:biosynthesis; D004353:Drug Evaluation, Preclinical; D004768:Enterotoxins / Q000037:antagonists & inhibitors*; D005260:Female; D005688:Galactosamine / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000187:drug effects; D052156:SELEX Aptamer Technique; D012772:Shock, Septic / Q000188:drug therapy / Q000382:microbiology
chemical_list: D016207:Cytokines; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D005688:Galactosamine