title: Different signal processing techniques of ratio spectra for spectrophotometric resolution of binary mixture of bisoprolol and hydrochlorothiazide; a comparative study.
abstract: Five signal processing techniques were applied to ratio spectra for quantitative determination of bisoprolol (BIS) and hydrochlorothiazide (HCT) in their binary mixture. The proposed techniques are Numerical Differentiation of Ratio Spectra (ND-RS), Savitsky-Golay of Ratio Spectra (SG-RS), Continuous Wavelet Transform of Ratio Spectra (CWT-RS), Mean Centering of Ratio Spectra (MC-RS) and Discrete Fourier Transform of Ratio Spectra (DFT-RS). The linearity of the proposed methods was investigated in the range of 2-40 and 1-22 μg/mL for BIS and HCT, respectively. The proposed methods were applied successfully for the determination of the drugs in laboratory prepared mixtures and in commercial pharmaceutical preparations and standard deviation was less than 1.5. The five signal processing techniques were compared to each other and validated according to the ICH guidelines and accuracy, precision, repeatability and robustness were found to be within the acceptable limit.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Eman S
authors.initials: ES
authors.lastname: Elzanfaly
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: said.hassan@pharma.cu.edu.eg.
authors.forename: Said A
authors.initials: SA
authors.lastname: Hassan
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Maissa Y
authors.initials: MY
authors.lastname: Salem
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt.
authors.forename: Badr A
authors.initials: BA
authors.lastname: El-Zeany
mesh_terms: D000959:Antihypertensive Agents / Q000032:analysis*; D017298:Bisoprolol / Q000032:analysis*; D004338:Drug Combinations; D005583:Fourier Analysis; D006852:Hydrochlorothiazide / Q000032:analysis*; D057230:Limit of Detection; D013053:Spectrophotometry / Q000379:methods; D058067:Wavelet Analysis
chemical_list: D000959:Antihypertensive Agents; D004338:Drug Combinations; D006852:Hydrochlorothiazide; D017298:Bisoprolol
keywords: Bisoprolol; Fourier Transform; Hydrochlorothiazide; Mean Centering; Savitsky–Golay; Wavelet Transform