title: Reactive short-chain leaf volatiles act as powerful inducers of abiotic stress-related gene expression.
abstract: Abiotic stresses cause serious damage to plants; therefore, plants undergo a complicated stress response through signal transduction originating from environmental stimuli. Here we show that a subset of short-chain leaf volatiles with an α, β-unsaturated carbonyl bond in their structure (reactive short-chain leaf volatiles, RSLVs) like (E)-2-hexenal and (E)-2-butenal can act as signal chemicals that strongly induce the gene expression of abiotic-related transcription factors, such as heat stress-related transcription factors (HSFA2, MBF1c) and other abiotic stress-related transcription factors (DREB2A, ZATs). RSLV-induced expression of HSFA2 and MBF1c was eliminated in HSFA1s-, known as heat stress response master regulators, knockout mutant, whereas those of DREB2A and ZATs were not, suggesting that the RSLV signaling pathway is composed of HSFA1-dependent and -independent pathways. RSLV treatment induced production of chaperon proteins, and the RSLV-treated Arabidopsis thus demonstrated enhanced abiotic stress tolerance. Because oxidative stress treatment enhanced RSLV production, we concluded that commonly found RSLVs produced by environmental stresses are powerful inducer of abiotic stress-related gene expression as oxidative stress signals.
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada, Hyogo 657-8501, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada, Hyogo 657-8501, Japan.
authors.forename: Mikiko
authors.initials: M
authors.lastname: Kunishima
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada, Hyogo 657-8501, Japan.
authors.forename: Masaharu
authors.initials: M
authors.lastname: Mizutani
authors.affiliation: Graduate School of Agricultural Science, Kobe University, Rokkodai 1-1, Nada, Hyogo 657-8501, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Sugimoto
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics / Q000502:physiology; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics; D018869:Heat-Shock Response / Q000235:genetics; D018384:Oxidative Stress / Q000235:genetics*; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000447:Aldehydes; D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C114579:DREB2A protein, Arabidopsis; C000620134:HSFA2 protein, Arabidopsis; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; C491466:MBF1 protein, Arabidopsis; D010940:Plant Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C519175:Zat7 protein, Arabidopsis; C010463:n-hexanal; C012796:2-butenal