title: Purification and characterization of a novel exopolysaccharides produced by Lactobacillus sp. Ca6.
abstract: This study was undertaken to investigate the ability of ten lactic acid bacterial strains to produce exopolysaccharides (EPS) on MRS broth containing 4% sucrose. A maximum EPS production yield of 2.4 g/l was obtained by strain Lactobacillus sp. Ca6. The results from thin layer chromatography (TLC) and high performance chromatography (HPLC) analyses showed that the EPS produced was a polymer of glucose. Further FTIR spectroscopic analysis revealed the presence of carboxyl, hydroxyl and amide groups corresponding to a typical EPS. In addition to EPS production, Lactobacillus sp. Ca6 displayed good probiotic properties (antimicrobial activities and sensitivity to several antibiotics) and resistance to acidic condition (pH 2) and 5% bile bovine. Overall, the findings indicate that this strain has a number of promising properties that make it a potential promising candidate for future application as a food additive.
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Trabelsi
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Sirine Ben
authors.initials: SB
authors.lastname: Slima
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia.
authors.forename: Hela
authors.initials: H
authors.lastname: Chaabane
authors.affiliation: Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address: riadh_fss@yahoo.fr.
authors.forename: Ben Salah
authors.initials: BS
authors.lastname: Riadh
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification* / Q000494:pharmacology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000900:Anti-Bacterial Agents; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Exopolysaccharide (EPS); FTIR spectroscopy; Probiotics