title: Isolation and expression of NAC genes during persimmon fruit postharvest astringency removal.
abstract: NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of "Mopan" persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed.
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 11016045@zju.edu.cn.
authors.forename: Ting
authors.initials: T
authors.lastname: Min
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. wmm@zju.edu.cn.
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Wuhan Polytechnic University, Changqing Campus, Wuhan 430023, China. hongxunwang1977@gmail.com.
authors.forename: Hongxun
authors.initials: H
authors.lastname: Wang
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 0013915@zju.edu.cn.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Liu
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. 21216047@zju.edu.cn.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. donald.grierson@nottingham.ac.uk.
authors.forename: Donald
authors.initials: D
authors.lastname: Grierson
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. xuerenyin@zju.edu.cn.
authors.forename: Xue-Ren
authors.initials: XR
authors.lastname: Yin
authors.affiliation: Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. akun@zju.edu.cn.
authors.forename: Kun-Song
authors.initials: KS
authors.lastname: Chen
mesh_terms: D000079:Acetaldehyde / Q000032:analysis / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002245:Carbon Dioxide / Q000494:pharmacology; D029788:Diospyros / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D005638:Fruit / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D012995:Solubility; D013634:Tannins / Q000302:isolation & purification; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D013634:Tannins; D002245:Carbon Dioxide; D000079:Acetaldehyde