title: Target profiling of zerumbone using a novel cell-permeable clickable probe and quantitative chemical proteomics.
abstract: Zerumbone is a phytochemical with diverse biological activities ranging from anti-inflammatory to anti-cancer properties; however, to date the cellular targets of this important compound have remained elusive. Here we report the global protein target spectrum of zerumbone in living cancer cells using competitive activity-based protein profiling of a novel cell-permeable clickable probe, combined with quantitative mass spectrometry.
authors.affiliation: Department of Chemistry, Imperial College London, South Kensington Campus, Exhibition Road, London SW7 2AZ, UK. e.tate@imperial.ac.uk.
authors.forename: Karunakaran A
authors.initials: KA
authors.lastname: Kalesh
authors.forename: James A
authors.initials: JA
authors.lastname: Clulow
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
mesh_terms: D002463:Cell Membrane Permeability; D019295:Computational Biology; D006367:HeLa Cells; D006801:Humans; D013058:Mass Spectrometry; D015335:Molecular Probes / Q000737:chemistry*; D040901:Proteomics; D012717:Sesquiterpenes / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D012717:Sesquiterpenes; C403304:zerumbone