title: NLP-12 engages different UNC-13 proteins to potentiate tonic and evoked release.
abstract: A neuropeptide (NLP-12) and its receptor (CKR-2) potentiate tonic and evoked ACh release at Caenorhabditis elegans neuromuscular junctions. Increased evoked release is mediated by a presynaptic pathway (egl-30 Gαq and egl-8 PLCβ) that produces DAG, and by DAG binding to short and long UNC-13 proteins. Potentiation of tonic ACh release persists in mutants deficient for egl-30 Gαq and egl-8 PLCβ and requires DAG binding to UNC-13L (but not UNC-13S). Thus, NLP-12 adjusts tonic and evoked release by distinct mechanisms.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and.
authors.forename: Zhitao
authors.identifier: http://orcid.org/0000-0002-2948-3339
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Department of Communication Sciences and Disorders, Emerson College, Boston, Massachusetts 02116.
authors.forename: Amy B
authors.initials: AB
authors.lastname: Vashlishan-Murray
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and kaplan@molbio.mgh.harvard.edu.
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Kaplan
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D017173:Caenorhabditis elegans; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism; D043802:GTP-Binding Protein alpha Subunits, Gq-G11 / Q000378:metabolism; D009469:Neuromuscular Junction / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D054799:Phospholipase C beta / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; D002352:Carrier Proteins; D004075:Diglycerides; C479565:Egl-30 protein, C elegans; D009479:Neuropeptides; C083141:phorbol ester binding protein; C517632:Egl-8 protein, C elegans; D054799:Phospholipase C beta; D043802:GTP-Binding Protein alpha Subunits, Gq-G11; D000109:Acetylcholine
keywords: C. elegans; CCK; Munc13; NLP-12; UNC-13; neuropeptide