title: Development of biomimetic tilapia collagen nanofibers for skin regeneration through inducing keratinocytes differentiation and collagen synthesis of dermal fibroblasts.
abstract: In this study, tilapia skin collagen sponge and electrospun nanofibers were developed for wound dressing. The collagen sponge was composed of at least two α-peptides, and its denaturation temperature was 44.99 °C. It did not change the number of spleen-derived lymphocytes in BALB/c mice, the ratio of CD4+/CD8+ lymphocytes, and the level of IgG or IgM in Sprague-Dawley rat. The contact angle, tensile strength, and weight loss temperature of collagen nanofibers were 21.2°, 6.72±0.44 MPa, and 300 °C, respectively. The nanofibers could promote the viabilities of human keratinocytes (HaCaTs) and human dermal fibroblasts (HDFs), inducing epidermal differentiation through the gene expression of involucrin, filaggrin, and type I transglutaminase of HaCaTs, and they could also accelerate migration of HaCaTs with the expression of matrix metalloproteinase-9 and transforming growth factor-β1 (TGF-β1). Besides, the nanofibers could upregulate the protien level of Col-I in HDFs both via a direct effect and TGF-β1 secreted from HaCaTs, thus facilitating the formation of collagen fibers. Furthermore, the collagen nanofibers stimulated the skin regeneration rapidly and effectively in vivo. These biological effects could be explained as the contributions from the biomimic extracellular cell matrix structure, hydrophilicity, and the multiple amino acids of the collagen nanofibers.
authors.affiliation: Shanghai Biomaterials Research & Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine , Shanghai 200023, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Zhou
authors.forename: Nanping
authors.initials: N
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xue
authors.forename: Tingting
authors.initials: T
authors.lastname: Ding
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
authors.forename: Jiao
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D001458:Bandages; D040761:Biomimetic Materials / Q000138:chemical synthesis; D002454:Cell Differentiation; D003094:Collagen / Q000737:chemistry*; D003357:Cosmetic Techniques; D005347:Fibroblasts / Q000166:cytology*; D000091344:Filaggrin Proteins; D015603:Keratinocytes / Q000166:cytology*; D022125:Lacerations / Q000628:therapy*; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D012867:Skin / Q000166:cytology; D017210:Tilapia
chemical_list: C000717249:FLG protein, human; D000091344:Filaggrin Proteins; D003094:Collagen
keywords: HaCaTs differentiation; TGF-β1; collagen synthesis; electrospinning tilapia collagen nanofibers; skin regeneration