title: The detoxification process, bioaccumulation and damage effect in juvenile white shrimp Litopenaeus vannamei exposed to chrysene.
abstract: This study aimed to evaluate the effect of chrysene (CHR) on detoxification enzymes, bioaccumulation and effect of CHR on biomolecule damage in different organs of the juvenile white shrimp Litopenaeus vannamei. In this study, juvenile white shrimp L. vannamei were exposed to CHR for 21 days at four different concentrations as 0, 0.3, 2.1 and 14.7 μg/L. Results showed that CHR bioaccumulation increased rapidly at first then reached a plateau. The activities of aryl hydrocarbon hydroxylase (AHH), 7-ethoxyresorufin O-deethylase (EROD), epoxide hydrolase (EH), glutathione-S-transferase (GST), sulfotransferase (SULT) and uridinediphosphate glucuronyltransferase (UGT) were induced and then became stable gradually. Moreover, 2.1 and 14.7 μg/L CHR treatments increased activity of superoxide dismutase (SOD) in gills and hepatopancreas, while total antioxidant capacity (T-AOC) and GSH/GSSG were suppressed after CHR exposure. Additionally, lipid peroxidation (LPO) levels, protein carbonyl (PC) contents and DNA damage were induced throughout the exposure period, and different trends were detected with time of exposure. Overall, these novel findings of CHR bioaccumulation and resulted toxicity demonstrate that CHR could affect the physical status of L. vannamei. This study will form a solid basis for a realistic extrapolation scientific data for aquaculture water monitoring and food security.
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xianyun
authors.initials: X
authors.lastname: Ren
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
authors.affiliation: Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D017756:Aquaculture; D002911:Chrysenes / Q000493:pharmacokinetics / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage; D005880:Gills / Q000187:drug effects* / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D043143:Hepatopancreas / Q000187:drug effects* / Q000378:metabolism; D015227:Lipid Peroxidation; D010084:Oxidation-Reduction; D033561:Penaeidae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D002911:Chrysenes; D014874:Water Pollutants, Chemical; C031180:chrysene; D019363:Cytochrome P-450 CYP1A1; D013482:Superoxide Dismutase; D005982:Glutathione Transferase
keywords: Bioaccumalation; Chrysene; Damage effect; Detoxification enzymes; Litopenaeus vannamei