title: Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation.
abstract: Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific signal by absorbing nonspecific fluorescence. Through in vitro tests, we confirmed that the AuFHAp probe was capable of detecting ALP levels related to osteoblast activity in living cells with high fluorescence intensity.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 305-806, Daejeon, Republic of Korea. jyjeong@kribb.re.kr chungbh@kribb.re.kr.
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Lim
authors.forename: Joo Oak
authors.initials: JO
authors.lastname: Keem
authors.forename: Hui-suk
authors.initials: HS
authors.lastname: Yun
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Jung
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007208:Indocyanine Green / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000201:enzymology*
chemical_list: D005456:Fluorescent Dyes; D006046:Gold; D017886:Durapatite; D000469:Alkaline Phosphatase; D007208:Indocyanine Green