title: [Establishment of a cell model of insulin-resistant 3T3-L1 adipocytes].
abstract: OBJECTIVE
  To investigate the optimal conditions for establishing insulin-resistant 3T3-L1 adipocytes.
  
  
  METHODS
  Dexamethason (DEX), 3-isobutyl-methylxanthine (IBMX) and different concentrations of insulin (10(-8), 10(-7), and 10(-6) mol·L(-1)) were used to induce 3T3-L1 preadipocytes into mature adipocytes identified by oil red O staining. We established insulin- resistant 3T3-L1 adipocytes cell model (IR-3T3-L1) by exposing the cells to 1µmol·L(-1) DEX, and the changes of glucose concen- tration in the cell culture were determined by glucose oxidase-peroxidase (GOD-POD) assay.
  
  
  RESULTS
  Treatment of 3T3-L1 cells with DEX, IBMX and 10(-6) mol·L(-1)) insulin for 9 days resulted in the differentiation of >90% of the cells into mature adipocytes. IR-3T3-L1 cells cultured for 96 h in the culture media containing 1 µmol·L(-1) DEX showed significantly increased glucose consumption (P=0.0003) as compared with the control group at 36 h (P<0.001).
  
  
  CONCLUSIONS
  3T3-L1 cells can be induced into mature adipocytes by exposure to 1 µmol·L(-1) DEX, 0.5 mmol·L(-1) IBMX and 10(-6) mol·L(-1)) insulin. A 96 h exposure to 1 µmol·L(-1) DEX can induce 3T3-L1 adipocytes to acquire insulin resistance that can be maintained for 36 h.
authors.affiliation: School of Pharmacy, Zunyi Medical University, Zunyi 563000, China.E-mail: niexuqiang@126.com.
authors.forename: Xu-Qiang
authors.initials: XQ
authors.lastname: Nie
authors.forename: Jian-Wen
authors.initials: JW
authors.lastname: Yang
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Shi
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Zhang
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Zhang
authors.forename: Ka
authors.initials: K
authors.lastname: Bian
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000737:chemistry; D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media / Q000737:chemistry; D003907:Dexamethasone / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D007328:Insulin / Q000737:chemistry; D007333:Insulin Resistance; D051379:Mice
chemical_list: D003470:Culture Media; D007328:Insulin; D003907:Dexamethasone; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine