title: Evaluation of antitumor activity and antioxidant status of Alternanthera brasiliana against Ehrlich ascites carcinoma in Swiss albino mice.
abstract: OBJECTIVE
  The main objective of the present study was to explore the antitumor activity of the ethyl acetate extract of the Alternanthera brasiliana (EAAB) and its antioxidant status against Ehrlich ascites carcinoma (EAC) in Swiss albino mice.
  
  
  METHODS
  Based on the preliminary in vitro cytotoxicity studies, EAAB was selected for anti-tumor and antioxidant effects. Anticancer activity of EAAB was evaluated against EAC in Swiss albino mice at the doses of 200 and 400 mg/kg. EAAB was administered for 14 consecutive days after induction of cancer. After 24 h of the last dose and 18 h of fasting, half of the mice were sacrificed and rest were kept alive for assessing any increase in life span. The antitumor effect of EAAB was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological and biochemical parameters of EAC bearing host. Furthermore, the antioxidant and histopathological parameters were evaluated.
  
  
  RESULTS
  EAAB treatment has shown significant decrease in tumor volume, viable cell count, tumor weight and elevated the life span of EAC tumor bearing mice in a dose dependent manner. In hematological profile count of RBC, hemoglobin, and WBC were found reverted to normal. EAAB also significantly decreased the levels of lipid peroxidation and significantly increased the levels of GSH, SOD and Catalase.
  
  
  CONCLUSIONS
  From the above results it may be concluded that EAAB has potent dose dependent antitumor activity and is comparable to that of 5-flourouracil.
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research- Guwahati (NIPER Guwahati), India.
authors.forename: Pavan Kumar
authors.initials: PK
authors.lastname: Samudrala
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research- Guwahati (NIPER Guwahati), India.
authors.forename: Bibin Baby
authors.initials: BB
authors.lastname: Augustine
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research- Guwahati (NIPER Guwahati), India.
authors.forename: Eshvendar Reddy
authors.initials: ER
authors.lastname: Kasala
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research- Guwahati (NIPER Guwahati), India.
authors.forename: Lakshmi Narendra
authors.initials: LN
authors.lastname: Bodduluru
authors.affiliation: College of Veterinary Sciences (CVSc), Khanapara, India.
authors.forename: Chandana
authors.initials: C
authors.lastname: Barua
authors.affiliation: Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research- Guwahati (NIPER Guwahati), India ; Pharmacology, Gauhati Medical College, Bhangagarh, Guwahati, India.
authors.forename: Mangala
authors.initials: M
authors.lastname: Lahkar
keywords: 5-Flourouracil; alternanthera brasiliana; antioxidant; antitumor; ehrlich ascites carcinoma