title: Subcutaneous vaccination with Porphyromonas gingivalis ameliorates periodontitis by modulating Th17/Treg imbalance in a murine model.
abstract: To date, Porphyromonas gingivalis (P. gingivalis) vaccination has been studied only in animals, and no effective prophylactic human periodontal vaccine has been developed, with the reason for the failure of prophylactic human periodontal vaccines unknown. T helper 17 cell (Th17)/regulatory T (Treg) cell responses play an important role in the development of periodontitis, and a Th17/Treg imbalance causes the pathogenesis of periodontitis. However, whether vaccination with P. gingivalis can prevent periodontitis through modulation of the Th17/Treg imbalance remains unknown. In this study, mice were subcutaneously vaccinated with formalin-killed P. gingivalis and then orally challenged with P. gingivalis. The vaccination protected the mice from alveolar bone resorption and inflammation. These protective effects might be ascribed to downregulation of Th17 cells and interleukin (IL)-17A production, upregulation of Treg and receptor activator of nuclear factor-kappa B ligand (RANKL)(+)CD4(+)T cells, and IL-10 and transforming growth factor-β1 production, and inhibition of lymphocyte proliferation. Our findings may provide a direction for the development of a vaccine or therapy against periodontitis by alteration of the Th17/Treg imbalance.
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China; Department of Periodontics, School of Stomatology of Liaoning Medical College, Jinzhou, Liaoning, China.
authors.forename: Linyuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Brain and Spinal Cord Injury Research, First Affiliated Hospital of Liaoning Medical College, Jinzhou, Liaoning, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Guan
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address: xiaoping_ba@126.com.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory of Congenital Malformation Research, Ministry of Health, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
mesh_terms: D016301:Alveolar Bone Loss / Q000209:etiology / Q000276:immunology / Q000517:prevention & control; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D016866:Bacteroidaceae Infections / Q000150:complications / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D056747:Immunomodulation; D007279:Injections, Subcutaneous; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D010518:Periodontitis / Q000209:etiology / Q000276:immunology / Q000517:prevention & control*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*; D058504:Th17 Cells / Q000276:immunology*; D014611:Vaccination
chemical_list: D001428:Bacterial Vaccines; D016207:Cytokines; D016328:NF-kappa B
keywords: Periodontitis; RANKL; Th17 cell; Treg cell; Vaccination