title: Quantitative determination of three pentacyclic triterpenes from five Swertia L. species endemic to Western Ghats, India, using RP-HPLC analysis.
abstract: Aim of this study was to identify pentacyclic triterpenoids betulinic acid (BA), oleanolic acid (OA) and ursolic acid (UA) from five Swertia species endemic to Western Ghats, which are used as substitutes as well as adulterants to Swertia chirayita. Our results indicate that the concentration of active compound vary largely among and within the species from different localities. OA was found in a higher amount in all species compared to BA and UA. From the results, it was clear that BA, OA and UA are present in the endemic species collected from Western Ghats, thus advocating the use of these species as alternate sources to S. chirayita. This in due course may release pressure of exploitation from natural resources of S. chirayita and help to bring it out from an endangered category from conservation point of view.
authors.affiliation: a Laboratory of Cytogenetics and Plant Breeding, Department of Botany , Shivaji University , Kolhapur 416 004 , MS , India.
authors.forename: Parthraj R
authors.initials: PR
authors.lastname: Kshirsagar
authors.forename: Sandeep R
authors.initials: SR
authors.lastname: Pai
authors.forename: Mansingraj S
authors.initials: MS
authors.lastname: Nimbalkar
authors.forename: Nikhil B
authors.initials: NB
authors.lastname: Gaikwad
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007194:India; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry* / Q000302:isolation & purification; D053978:Pentacyclic Triterpenes; D010946:Plants, Medicinal / Q000737:chemistry; D013045:Species Specificity; D029967:Swertia / Q000737:chemistry* / Q000145:classification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification; D000094062:Betulinic Acid; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid; D000097245:Ursolic Acid
keywords: RP-HPLC; Swertia; betulinic acid; oleanolic acid; ursolic acid