title: Studies on the antimicrobial potential and structural characterization of fatty acids extracted from Sydney rock oyster Saccostrea glomerata.
abstract: BACKGROUND
  The marine environment having vast resources of natural products with potential bioactivities. Among the marine natural products, fatty acids obtained from marine mollusks have broad range of biological activities including antimicrobial and antitumor activities. The present study aims to characterize the fatty acid derivatives from the Sydney rock oyster Saccostrea glomerata and its pharmacological activities.
  
  
  METHODS
  S. glomerata fleshes were serially extracted with hexane, ethyl acetate and methanol and studied the antimicrobial activities against pathogenic bacteria, fungi and virus. Based on the better result, the ethyl acetate extract was selected and purified through silica column chromatography and screened the fractions for antimicrobial and antitumor activities. Also the best active fraction (FV) was functionally and structurally characterized.
  
  
  RESULTS
  The ethyl acetate extract of S. glomerata effectively controlled the bacterial pathogens and formed of more than 15 mm of zone of inhibition and also effectively suppressed the fungal growth and inhibit the shrimp white spot syndrome virus (WSSV). The secondary screening results revealed that, the fraction (FV) had potential antimicrobial and antitumor activities. The FV concentration (100 μg/ml) effectively suppressed the tumor mammary epithelial carcinoma cell of 14.45%. The GC-MS analysis revealed that, eleven compounds including N-hexadecanoic acid, L-(+)-ascorbic acid 2,6-dihexadecanoate and 6-Octadecenoic acid were characterized.
  
  
  CONCLUSIONS
  The fatty acid derivatives isolated and characterized from S. glomerata extracts had the potent antimicrobial and antitumor activities. This basic research can help to develop the antimicrobial and anticancer drugs from the nutraceuticals in future.
authors.forename: Subbiahanadar Chelladurai
authors.initials: SC
authors.lastname: Karthikeyan
authors.forename: Subramanian
authors.initials: S
authors.lastname: Velmurugan
authors.forename: Mariathason Birdilla Selva
authors.initials: MB
authors.lastname: Donio
authors.forename: Mariavincent
authors.initials: M
authors.lastname: Michaelbabu
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari 629502, Tamilnadu, India. citarasu@gmail.com.
authors.forename: Thavasimuthu
authors.initials: T
authors.lastname: Citarasu
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology; D059001:Aquatic Organisms / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology; D002457:Cell Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002853:Chromatography, Liquid; D005227:Fatty Acids / Q000302:isolation & purification / Q000494:pharmacology*; D005658:Fungi / Q000187:drug effects*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008826:Microbial Sensitivity Tests; D010125:Ostreidae / Q000737:chemistry*; D046848:White spot syndrome virus 1 / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D001688:Biological Products; D002457:Cell Extracts; D005227:Fatty Acids