title: Structural changes of linear DNA molecules induced by cisplatin.
abstract: Interaction between long DNA molecules and activated cisplatin is believed to be crucial to anticancer activity. However, the exact structural changes of long DNA molecules induced by cisplatin are still not very clear. In this study, structural changes of long linear double-stranded DNA (dsDNA) and short single-stranded DNA (ssDNA) induced by activated cisplatin have been investigated by atomic force microscopy (AFM). The results indicated that long DNA molecules gradually formed network structures, beads-on-string structures and their large aggregates. Electrostatic and coordination interactions were considered as the main driving forces producing these novel structures. An interesting finding in this study is the beads-on-string structures. Moreover, it is worth noting that the beads-on-string structures were linked into the networks, which can be ascribed to the strong DNA-DNA interactions. This study expands our knowledge of the interactions between DNA molecules and cisplatin.
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China. Electronic address: cn.zguoliu@yahoo.com.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Ruisi
authors.initials: R
authors.lastname: Liu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
authors.affiliation: State Engineering Laboratory of Bio-Resource Eco-Utilization, Harbin 150040, People's Republic of China; Engineering Research Center of Forest Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China; Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, People's Republic of China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry* / Q000648:ultrastructure; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000648:ultrastructure; D018625:Microscopy, Atomic Force; D009690:Nucleic Acid Conformation / Q000187:drug effects*
chemical_list: D000970:Antineoplastic Agents; D004277:DNA, Single-Stranded; D004247:DNA; D002945:Cisplatin
keywords: Atomic force microscopy (AFM); Cisplatin; DNA; HOPG