title: Temporal and spatial expression analysis of peripheral myelin protein 22 (Pmp22) in developing Xenopus.
abstract: Peripheral myelin protein 22 (Pmp22), a member of the junction protein family Claudin/EMP/PMP22, contributes to the formation and maintenance of myelin sheaths in the peripheral nervous system. Apart from the establishment and maintenance of peripheral nerves, Pmp22 and its family member have also participated in a broad range of more general processes including cell cycle regulation and apoptosis during development. Pmp22 has been identified from several vertebrate species including mouse, human and zebrafish. However, Pmp22 has not been identified from Xenopus embryos yet. In this paper, we cloned Pmp22 from Xenopus laevis and evaluated its expression during embryogenesis. We found that Pmp22 was initially expressed in the mesoderm and cement gland during the neurula stage. At early tailbud stage, strong expression of Pmp22 was detected in the trigeminal and profundal ganglia as well as developing somites and branchial arches. Later in development, Pmp22 was expressed specifically in cranio-facial cartilage, roof plate and floor plate of the developing brain, otic vesicle and lens. Pmp22 is also strongly expressed in the developing trachea and lungs. Based on its expression in facial tissues, we propose that Pmp22 may be involved in the formation of head structure in addition to the maintenance of functional peripheral nerves in Xenopus embryos.
authors.affiliation: Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, 1 Hallymdaehak-gil, Chunchon 200-702, South Korea.
authors.forename: Hyun-Jin
authors.initials: HJ
authors.lastname: Tae
authors.affiliation: Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, 567 Baekje-Daero, Jeonju 561-756, Republic of Korea.
authors.forename: Md Mahfujur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, 567 Baekje-Daero, Jeonju 561-756, Republic of Korea. Electronic address: parkb@jbnu.ac.kr.
authors.forename: Byung-Yong
authors.initials: BY
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002356:Cartilage / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D009185:Myelin Proteins / Q000737:chemistry / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014981:Xenopus / Q000254:growth & development*; D029867:Xenopus Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009185:Myelin Proteins; D029867:Xenopus Proteins; C000602194:peripheral myelin protein 22, Xenopus
keywords: Cranio-facial cartilages; Otic vesicle; Pmp22; Trigeminal ganglia; Xenopus