title: Proteomics approach reveals mechanism underlying susceptibility of loquat fruit to sunburn during color changing period.
abstract: The objective of this work was to investigate why loquat fruit peels are more sensitive to high temperature and strong sunlight, making them highly susceptible to sunburn, during the color changing period (CCP). Two dimensional gel electrophoresis (2-DE) of the fruit peel proteins was performed over three developmental periods, namely green fruit period (GFP), color changing period and yellow ripening period (YRP). Fifty-five protein spots with at least 2-fold differences in abundance were successfully identified by MALDI-TOF-TOF/MS. The identified proteins were divided into categories related to heat-shock response, stress response and defense, energy metabolism, photosynthesis and protein biosynthesis. The results showed that expression of proteins related to anaerobic respiration and photorespiration were increased while the proteins related to ROS scavenging, polyamine biosynthesis, defense pathogens and photosynthesis were decreased during CCP under heat stress. Our findings provide new insights into the molecular mechanism of loquat fruit susceptible to sunburn during CCP.
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China; Fujian Breeding Engineering Technology Center for Longan & Loquat, Fuzhou 350013, China.
authors.forename: Ji-Mou
authors.initials: JM
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Yong-Xiang
authors.initials: YX
authors.lastname: Lin
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Yi-Yong
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China; Fujian Breeding Engineering Technology Center for Longan & Loquat, Fuzhou 350013, China.
authors.forename: Chao-Jun
authors.initials: CJ
authors.lastname: Deng
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Hui-Wen
authors.initials: HW
authors.lastname: Gong
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China; Fujian Breeding Engineering Technology Center for Longan & Loquat, Fuzhou 350013, China.
authors.forename: Qi-Zhi
authors.initials: QZ
authors.lastname: Xu
authors.affiliation: Fruit Research Institute of Fujian Academy of Agriculture Science, Fuzhou 350013, China; Fujian Breeding Engineering Technology Center for Longan & Loquat, Fuzhou 350013, China. Electronic address: zsq333555@163.com.
authors.forename: Shao-Quan
authors.initials: SQ
authors.lastname: Zheng
authors.affiliation: School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Fujian Provincial Key Laboratory of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: weichen909@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D031983:Eriobotrya / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D018506:Gene Expression Regulation, Plant; D006358:Hot Temperature; D010940:Plant Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013471:Sunburn / Q000382:microbiology*
chemical_list: D010940:Plant Proteins
keywords: Heat stress; Loquat fruit; MALDI-TOF–TOF/MS; Proteome; Sunburn