title: Candida dubliniensis and Candida albicans differentiation by colony morphotype in Sabouraud-triphenyltetrazolium agar.
abstract: BACKGROUND
  Candida dubliniensis is a germ tube and chlamydoconidia producing Candida species that may be misidentified as Candida albicans. Molecular-based methods are the most reliable techniques for C. albicans and C. dubliniensis differentiation. However, accurate, quick and inexpensive phenotypic tests are needed to be used in low-complexity mycology laboratories.
  
  
  OBJECTIVE
  To evaluate colony morphotypes on Sabouraud-triphenyltetrazolium agar as a tool for C. dubliniensis and C. albicans differentiation.
  
  
  METHODS
  The morphology of 126 C. albicans and C. dubliniensis strains was evaluated and compared with their identification by molecular methods.
  
  
  RESULTS
  The method showed 100% sensitivity and specificity when color and the presence or absence of large white mycelial halo was evaluated.
  
  
  CONCLUSIONS
  Colony morphotype on Sabouraud-triphenyltetrazolium agar should be considered as a new tool to differentiate C. dubliniensis and C. albicans.
authors.affiliation: Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Soledad
authors.initials: S
authors.lastname: Gamarra
authors.affiliation: Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Mancilla
authors.affiliation: Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Catiana
authors.initials: C
authors.lastname: Dudiuk
authors.affiliation: Laboratorio de Micología y Diagnóstico Molecular, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CONICET, Santa Fe, Argentina; Catedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: ggarcia@unl.edu.ar.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Garcia-Effron
mesh_terms: D002175:Candida / Q000254:growth & development*; D002176:Candida albicans / Q000254:growth & development; D003116:Color; D003470:Culture Media; D025282:Mycelium / Q000648:ultrastructure; D016533:Mycological Typing Techniques; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013778:Tetrazolium Salts
chemical_list: D003470:Culture Media; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium
keywords: Candida albicans; Candida dubliniensis; Sales de trifeniltetrazolio; Triphenyltetrazolium salt