title: MicroRNA-10b is upregulated and has an invasive role in colorectal cancer through enhanced Rhoc expression.
abstract: We found that the difference in miR‑10b expression between the tumor tissue and adjacent non‑tumor tissue was significant. Outer periphery and portal vein serum miR‑10b concentrations were significantly higher than those of the control. However, the outer periphery vein miR‑10b concentrations were not significant when compared with the portal vein serum concentration in colorectal cancer. The expression levels of miR‑10b were associated with higher‑grade colorectal cancer. MiR‑10b levels were markedly elevated in lymph node metastasis-positive tumor tissue compared with those in lymph node metastasis-free tumor tissue, and were correlated with a downregulation in Hoxd10 expression. Rhoc protein expression in tumor tissue was significantly amplified when compared to that of the control tissue group. An inverse correlation between Hoxd10 and Rhoc in immunohistochemistry and western blot analysis was observed (P<0.05). MiR-10b expression was also inversely correlated with Hoxd10 protein expression (P<0.05). Thus, miR‑10b is potentially involved in the invasion of colorectal cancer.
authors.affiliation: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.affiliation: Central Experiment Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Xu-Hong
authors.initials: XH
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Zuo
authors.affiliation: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 201620, P.R. China.
authors.forename: Zhi-Hai
authors.initials: ZH
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D018398:Homeodomain Proteins / Q000096:biosynthesis*; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics*; D011169:Portal Vein / Q000378:metabolism; D014157:Transcription Factors / Q000096:biosynthesis*; D020741:rho GTP-Binding Proteins / Q000096:biosynthesis*; D000075024:rhoC GTP-Binding Protein
chemical_list: D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; C547183:MIRN10 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors; C072064:HOXD10 protein, human; C066690:RHOC protein, human; D020741:rho GTP-Binding Proteins; D000075024:rhoC GTP-Binding Protein