title: Neuronal cell reconstruction with umbilical cord blood cells in the brain hypoxia-ischemia.
abstract: Brain hypoxia-ischemia is a human neonatal injury that is considered a candidate for stem cell therapy.
  
  
  
  The possible therapeutic potential of human umbilical cord blood (HUCB) stem cells was evaluated in 14-day-old rats subjected to the right common carotid occlusion, a model of neonatal brain hypoxia-ischemia. Seven days after hypoxia-ischemia, rats received either saline solution or 4 × 105 HUCB cells i.v. Rats in control group did not receive any injection. After two weeks, rats were assessed using two motor tests. Subsequently, rats were scarified for histological and immunohistochemical analyses.
  
  
  
  Our immunohistochemical findings demonstrated selective migration of the injected HUCB cells to the ischemic area as well as reduction in infarct volume. Seven days after surgery, we found significant recovery in the behavioral performance in the test group (12.7 +/- 0.3) compared to the sham group (10.0 +/-0.05), a trend which continued to day 14 (15.3 ± 0.3 vs. 11.9 ± 0.5, P<0.05). Postural and motor asymmetries at days 7 and 14 in the test group showed a significant decrease in the percentage of right turns in comparison to the sham group (75% and 59% vs. 97% and 96%, P<0.05).
  
  
  
  The results show the potential of HUCB stem cells in reduction of neurologic deficits associated with neonatal hypoxia-ischemia.
authors.affiliation: Dept. of Pediatrics, Medical School, Zabol Medical Science University, Zabol, Iran.
authors.forename: Hossein Ali
authors.initials: HA
authors.lastname: Ghaffaripour
authors.affiliation: Dept. of Anatomy and Cell Biology, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Jalali
authors.affiliation: Dept. of Anatomy and Cell Biology, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nikravesh
authors.affiliation: Dept. of Anatomy and Cell Biology, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Seghatoleslam
authors.affiliation: Dept. of Biochemistry and Immunology, Medical School, Zabol Medical Science University, Zabol, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Sanchooli
mesh_terms: D000328:Adult; D000818:Animals; D000831:Animals, Newborn; D001522:Behavior, Animal / Q000502:physiology; D001921:Brain / Q000166:cytology* / Q000473:pathology; D002465:Cell Movement; D036101:Cord Blood Stem Cell Transplantation; D004195:Disease Models, Animal; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D053686:Fetal Stem Cells / Q000166:cytology / Q000637:transplantation*; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000473:pathology* / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D055815:Young Adult
keywords: Hypoxia-Ischemia; Nerve cell; Umbilical Cord Blood