title: The Effects of Hydroalcoholic Extract of Apium graveolens Leaf on the Number of Sexual Cells and Testicular Structure in Rat.
abstract: BACKGROUND
  Use of medicinal plants with high antioxidant properties could be effective to increase fertility and improvement of disorders such as hormonal imbalance, impotency, oligospermia and immotile sperm. Celery (Apium graveolens) is rich in antioxidant agents. The leaf and stems of celery contain phenols, furanocoumarin and luteolin. Apigenin is one of the main flavonoids of celery leaf.
  
  
  OBJECTIVE
  This study aimed to investigate the effects of hydroalcoholic extract of celery on histological properties of testis and number of sexual cells in male rats.
  
  
  METHODS
  Thirty-two male Wistar rats were divided into four groups of eight rats each. Control, did not receive any medication; sham, received normal saline; and two groups received celery extract orally in dosages of 100 and 200 mg/kg/BW once every two days for 60 days. At the end, animals were anesthetized, and caudal part of the right epididymis was used for sperm counting. After fixation of testis, tissue sections were prepared and studied microscopically to evaluate morphometric (lumen diameter, number of primary spermatocyte and sertoli cell) and histological changes. Data was analyzed by one-way ANOVA test using SPSS15 software. P < 0.05 was considered as statistically significant.
  
  
  RESULTS
  There was a significant increase in the number of sperms, sertoli cells, and primary spermatocyte (P < 0.05) in groups receiving extract; however, structural changes were not observed in the groups.
  
  
  CONCLUSIONS
  It seems that celery increases spermatogenesis in male rats, but has no destructive effects on testicular tissue.
authors.affiliation: Department of Immunology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, IR Iran ; Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, IR Iran.
authors.forename: Wesam
authors.initials: W
authors.lastname: Kooti
authors.affiliation: Cellular and Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Esrafil
authors.initials: E
authors.lastname: Mansouri
authors.affiliation: Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghasemiboroon
authors.affiliation: Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Harizi
authors.affiliation: Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran ; Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Damoon
authors.initials: D
authors.lastname: Ashtary-Larky
authors.affiliation: Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Afrisham
keywords: Apium graveolens; Extract; Rat; Testicular Structure