title: PmrD is required for modifications to escherichia coli endotoxin that promote antimicrobial resistance.
abstract: In Salmonella enterica, PmrD is a connector protein that links the two-component systems PhoP-PhoQ and PmrA-PmrB. While Escherichia coli encodes a PmrD homolog, it is thought to be incapable of connecting PhoPQ and PmrAB in this organism due to functional divergence from the S. enterica protein. However, our laboratory previously observed that low concentrations of Mg(2+), a PhoPQ-activating signal, leads to the induction of PmrAB-dependent lipid A modifications in wild-type E. coli (C. M. Herrera, J. V. Hankins, and M. S. Trent, Mol Microbiol 76:1444-1460, 2010, http://dx.doi.org/10.1111/j.1365-2958.2010.07150.x). These modifications include phosphoethanolamine (pEtN) and 4-amino-4-deoxy-l-arabinose (l-Ara4N), which promote bacterial resistance to cationic antimicrobial peptides (CAMPs) when affixed to lipid A. Here, we demonstrate that pmrD is required for modification of the lipid A domain of E. coli lipopolysaccharide (LPS) under low-Mg(2+) growth conditions. Further, RNA sequencing shows that E. coli pmrD influences the expression of pmrA and its downstream targets, including genes coding for the modification enzymes that transfer pEtN and l-Ara4N to the lipid A molecule. In line with these findings, a pmrD mutant is dramatically impaired in survival compared with the wild-type strain when exposed to the CAMP polymyxin B. Notably, we also reveal the presence of an unknown factor or system capable of activating pmrD to promote lipid A modification in the absence of the PhoPQ system. These results illuminate a more complex network of protein interactions surrounding activation of PhoPQ and PmrAB in E. coli than previously understood.
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Erica J
authors.initials: EJ
authors.lastname: Rubin
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Carmen M
authors.initials: CM
authors.lastname: Herrera
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Crofts
authors.affiliation: Department of Molecular Biosciences, The University of Texas at Austin, Austin, Texas, USA Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas, USA Center for Infectious Disease, The University of Texas at Austin, Austin, Texas, USA strent@austin.utexas.edu.
authors.forename: M Stephen
authors.initials: MS
authors.lastname: Trent
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular; D024881:Drug Resistance, Bacterial / Q000187:drug effects / Q000235:genetics*; D004731:Endotoxins / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D008050:Lipid A / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D004731:Endotoxins; D029968:Escherichia coli Proteins; D008050:Lipid A; D008070:Lipopolysaccharides; D010714:Phosphatidylethanolamines; C523605:PmrD protein, E coli; D012329:RNA, Bacterial; C483858:phosphatidylethanolamine; C044101:endotoxin, Escherichia coli; D008274:Magnesium; D011112:Polymyxin B