title: Romboutsia sedimentorum sp. nov., isolated from an alkaline-saline lake sediment and emended description of the genus Romboutsia.
abstract: A Gram-stain-positive, spore-forming, obligately anaerobic bacterium, designated LAM201(T), was isolated from sediment samples from an alkaline-saline lake located in Daqing oilfield, Daqing City, PR China. Cells of strain LAM201(T) were non-motile and straight or spiral rod-shapes. Strain LAM201(T) was able to utilize glucose, fructose, maltose, trehalose and sorbitol as the sole carbon source. Acetic acid, ethanol, iso-butanoic acid and iso-valeric acid were the main products of glucose fermentation. The major fatty acids of LAM201(T) were C(16 : 0) (26.7%) and C(18 : 0) (11.2%). The main polar lipids were four unknown glycolipids and five unknown phospholipids. The predominant cell-wall sugars were ribose and galactose. The cell-wall peptidoglycan of strain LAM201(T) contained alanine, glycine, glutamic acid and aspartic acid. Sodium sulfite was used as the electron acceptor. The G+C content of the genomic DNA was 32±0.8 mol%, as determined by the T(m) method. Analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Romboutsia and was most closely related to Romboutsia lituseburensis DSM 797(T) and Romboutsia ilealis CRIB(T) with 97.3% and 97.2% similarities, respectively. The DNA-DNA hybridization values between strain LAM201(T) and the two reference strains were 37% and 31%, respectively. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM201(T) is suggested to represent a novel species within the genus Romboutsia , for which the name Romboutsia sedimentorum sp. nov. is proposed. The type strain is LAM201(T) ( = ACCC 00717(T) = JCM 19607(T)).
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Yanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Song
authors.affiliation: Agricultural Engineering Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Winclove Probiotics, Hulstweg 11, 1032 LB Amsterdam, The Netherlands.|Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
authors.forename: Jacoline
authors.initials: J
authors.lastname: Gerritsen
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Bingqiang
authors.initials: B
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Microbial Resources (Ministry of Agriculture, PR China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, PR China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Ruan
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology; D006017:Glycolipids / Q000737:chemistry; D016937:Gram-Positive Endospore-Forming Rods / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S