title: Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.
abstract: We have developed a versatile new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization-dependent fluorescent proteins. We demonstrate the use of this strategy to construct both intermolecular and intramolecular ratiometric biosensors for qualitative imaging of caspase activity, Ca(2+) concentration dynamics and other second-messenger signaling activities.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, PKU (Peking University)-IDG (International Data Group)/McGovern Institute for Brain Research, Peking University, Beijing, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Jhon Ralph
authors.initials: JR
authors.lastname: Enterina
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Issan
authors.initials: I
authors.lastname: Zhang
authors.affiliation: Montana Molecular, Bozeman, Montana, USA.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Tewson
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Gary C H
authors.initials: GC
authors.lastname: Mo
authors.affiliation: Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Montana Molecular, Bozeman, Montana, USA.
authors.forename: Anne Marie
authors.initials: AM
authors.lastname: Quinn
authors.affiliation: 1] Montana Molecular, Bozeman, Montana, USA. [2] Department of Cell Biology and Neuroscience, Montana State University, Bozeman, Montana, USA.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Hughes
authors.affiliation: Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
authors.forename: Dusica
authors.initials: D
authors.lastname: Maysinger
authors.affiliation: 1] Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada. [2] Department of Bioengineering, Stanford University, Stanford, California, USA.
authors.forename: Spencer C
authors.initials: SC
authors.lastname: Alford
authors.affiliation: State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, PKU (Peking University)-IDG (International Data Group)/McGovern Institute for Brain Research, Peking University, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Robert E
authors.identifier: 000000030604092X
authors.initials: RE
authors.lastname: Campbell
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000378:metabolism; D053148:Caspase 3 / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods; D055503:Protein Multimerization; D000097573:Red Fluorescent Protein
chemical_list: D002118:Calcium; D002147:Calmodulin; D053148:Caspase 3; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein