title: The susceptibility of primate lentiviruses to nucleosides and Vpx during infection of dendritic cells is regulated by CA.
abstract: The block toward human immunodeficiency virus type 1 (HIV-1) infection of dendritic cells (DCs) can be relieved by Vpx (viral protein X), which degrades sterile alpha motif-hydroxylase domain 1 (SAMHD1) or by exogenously added deoxynucleosides (dNs), lending support to the hypothesis that SAMHD1 acts by limiting deoxynucleoside triphosphates (dNTPs). This notion has, however, been questioned. We show that while dNs and Vpx increase the infectivity of HIV-1, only the latter restores the infectivity of a simian immunodeficiency virus of macaques variant, SIVMACΔVpx virus. This distinct behavior seems to map to CA, suggesting that species-specific CA interactors modulate infection of DCs.
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Barateau
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France.
authors.forename: Xuan-Nhi
authors.initials: XN
authors.lastname: Nguyen
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Bourguillault
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Berger
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Cordeil
authors.affiliation: Centre International de Recherche en Infectiologie (CIRI), Lyon, France INSERM, U1111, Lyon, France Ecole Normale Supérieure de Lyon, Lyon, France CNRS, UMR5308, Lyon, France University of Lyon, Lyon I, UMS3444/US8 BioSciences Gerland, Lyon, France acimarel@ens-lyon.fr.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cimarelli
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000821:virology*; D015497:HIV-1 / Q000254:growth & development / Q000502:physiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008251:Macaca; D009705:Nucleosides / Q000378:metabolism*; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000502:physiology*; D054334:Viral Regulatory and Accessory Proteins / Q000378:metabolism*
chemical_list: D036022:Capsid Proteins; D009705:Nucleosides; C513059:VPX protein, Simian immunodeficiency virus; D054334:Viral Regulatory and Accessory Proteins