title: Lack of association between glutathione peroxidase1 (GPx1) activity, Pro198Leu polymorphism and stenosis of coronary arteries: A population-based prediction.
abstract: BACKGROUND
  We studied the association between erythrocyte glutathione peroxidase1 (GPx1) activity and rs1050450 (Pro198Leu) site with the stenosis of coronary arteries and, evaluated the Pro/Leu position within the predicted tertiary structure.
  
  
  METHODS
  Subjects were recruited from who underwent coronary angiography (controls; n = 55, Stenosis < 5% and Patients; n = 95, Stenosis ≥ 50%). The GPx1 activity and rs1050450 C/T variants were determined using enzyme assay and RFLP-PCR techniques, respectively. The conserved regions and GPx1 tertiary structure were predicted using bioinformatics tools.
  
  
  RESULTS
  We did not find significant association between GPx1 activity (P = 0.96), rs1050450 genotype distribution and coronary artery disease (adjusted OR = 0.79; 95%CI 0.28-2.2, P = 0.6). The polymorphic variants were not located at the predicted structural and functional domains so that it had not the significant role on the GPx1stability and function.
  
  
  CONCLUSIONS
  In agreement with the results predicted from bioinformatics tools, we suggested that the GPx1 activity and rs1050450 (Pro198Leu) site are not involved in the development of stenosis of coronary arteries in the study population.
authors.affiliation: Cellular and Molecular Research Center, Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Najafi
authors.affiliation: Biochemistry Department, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Hassan
authors.initials: H
authors.lastname: Ghasemi
authors.affiliation: Biochemistry Department, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Abazar
authors.initials: A
authors.lastname: Roustazadeh
authors.affiliation: Cellular and Molecular Research Center, School of Medical Biotechnology, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Farajollahi
keywords: Bioinformatics; Coronary artery disease; Glutathione peroxidase1 (GPx1); rs1050450 (Pro198Leu)