title: The Omp85-type outer membrane protein p36 of Arabidopsis thaliana evolved by recent gene duplication.
abstract: Proteins of the Omp85 family are involved in the insertion of β-barrel shaped outer membrane proteins in bacteria and mitochondria, and-at least-in the transfer of preproteins across the chloroplast outer envelope. In general these proteins consist of up to five N-terminal "polypeptide transport associated" (POTRA) domains and a C-terminal, membrane embedded β-barrel domain. In Arabidopsis thaliana two plastidic gene families coding for Omp85-like proteins exist, namely the Toc75-III and the Toc75-V/Oep80 sub-family. The latter is composed of three genes, of which two do not contain POTRA domains. These are annotated as P39 and P36. However, P36 resulted from a very recent gene duplication of P39 and appears to be specific to Arabidopsis thaliana. Furthermore, we show that P39 is specifically expressed in vein tissues, while P36 is expressed at early and late developmental stages. T-DNA insertion in P36 causes a mild phenotype with reduced starch accumulation in chloroplasts of sepals pointing towards a yet to be described plastid function.
authors.affiliation: Department of Molecular Cell Biology of Plants, Goethe University, Max-von-Laue Str. 9, 60438, Frankfurt, Germany, kerstin.nicolaisen@gmx.de.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Nicolaisen
authors.forename: Sandra
authors.initials: S
authors.lastname: Missbach
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Hsueh
authors.forename: Franziska
authors.initials: F
authors.lastname: Ertel
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Fulgosi
authors.forename: Maik S
authors.initials: MS
authors.lastname: Sommer
authors.forename: Enrico
authors.initials: E
authors.lastname: Schleiff
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D019143:Evolution, Molecular; D020440:Gene Duplication; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D018087:Plastids / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; C532780:OEP80 protein, Arabidopsis