title: Preparation of 17β-estradiol-imprinted material by surface-initiated atom transfer radical polymerization and its application.
abstract: A novel 17β-estradiol molecularly imprinted polymer was grafted onto the surface of initiator-immobilized silica by surface-initiated atom transfer radical polymerization. The resulting molecularly imprinted polymer was characterized by elemental analysis and thermogravimetric analysis. The binding property of molecularly imprinted polymer for 17β-estradiol was also studied with both static and dynamic methods. The results showed that the molecularly imprinted polymer possessed excellent recognition capacity for 17β-estradiol (180.65 mg/g at 298 K), and also exhibited outstanding selectivity for 17β-estradiol over the other competitive compounds (such as testosterone and progesterone). Then, the determination of trace 17β-estradiol in beef samples was successfully developed by using molecularly imprinted polymer solid-phase extraction coupled with high-performance liquid chromatography. The limit of detection was 0.25 ng/mL, and the amount of 17β-estradiol in beef samples was detected at 2.83 ng/g. This work proposed a sensitive, rapid, reliable, and convenient approach for the determination of trace 17β-estradiol in complicated beef samples.
authors.affiliation: School of Chemistry and Chemical Engineering, Beifang University of Nationalities, Yinchuan, China; Key Laboratory of Energy and Chemical Engineering, Ningxia University, Yinchuan, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Gong
authors.forename: Yuling
authors.initials: Y
authors.lastname: Niu
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Gong
authors.forename: Meihua
authors.initials: M
authors.lastname: Ma
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ren
authors.forename: Weihua
authors.initials: W
authors.lastname: Zhu
authors.forename: Ruiming
authors.initials: R
authors.lastname: Luo
authors.forename: Bolin
authors.initials: B
authors.lastname: Gong
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D004958:Estradiol / Q000737:chemistry*; D007700:Kinetics; D008461:Meat Products / Q000032:analysis; D058105:Polymerization; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013818:Thermogravimetry
chemical_list: D004958:Estradiol
keywords: 17β-Estradiol; Atom transfer radical polymerization; Beef samples; Molecularly imprinted polymers; Solid-phase extraction