title: Design, construction and mechanical testing of digital 3D anatomical data-based PCL-HA bone tissue engineering scaffold.
abstract: The study aims to investigate the techniques of design and construction of CT 3D reconstructional data-based polycaprolactone (PCL)-hydroxyapatite (HA) scaffold. Femoral and lumbar spinal specimens of eight male New Zealand white rabbits were performed CT and laser scanning data-based 3D printing scaffold processing using PCL-HA powder. Each group was performed eight scaffolds. The CAD-based 3D printed porous cylindrical stents were 16 piece × 3 groups, including the orthogonal scaffold, the Pozi-hole scaffold and the triangular hole scaffold. The gross forms, fiber scaffold diameters and porosities of the scaffolds were measured, and the mechanical testing was performed towards eight pieces of the three kinds of cylindrical scaffolds, respectively. The loading force, deformation, maximum-affordable pressure and deformation value were recorded. The pore-connection rate of each scaffold was 100 % within each group, there was no significant difference in the gross parameters and micro-structural parameters of each scaffold when compared with the design values (P > 0.05). There was no significant difference in the loading force, deformation and deformation value under the maximum-affordable pressure of the three different cylinder scaffolds when the load was above 320 N. The combination of CT and CAD reverse technology could accomplish the design and manufacturing of complex bone tissue engineering scaffolds, with no significant difference in the impacts of the microstructures towards the physical properties of different porous scaffolds under large load.
authors.affiliation: Department of Orthopaedics, Nanjing Medical University Nanjing Hospital, Nanjing, 210006, China.
authors.forename: Qingqiang
authors.initials: Q
authors.lastname: Yao
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.forename: Chengzhe
authors.initials: C
authors.lastname: Jin
authors.forename: Xiaotao
authors.initials: X
authors.lastname: Du
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Junwei
authors.initials: J
authors.lastname: Yan
authors.forename: Wenhao
authors.initials: W
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhou
authors.forename: Yijin
authors.initials: Y
authors.lastname: Wang
authors.forename: Liming
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D018786:Bone Substitutes; D001842:Bone and Bones / Q000473:pathology*; D017886:Durapatite / Q000737:chemistry*; D005269:Femur / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D066330:Printing, Three-Dimensional; D011817:Rabbits; D011859:Radiography; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite