title: A reproducible method for the expansion of mouse CD8+ T lymphocytes.
abstract: Murine adoptive CD8+ T-cell immunotherapy studies require the generation of large numbers of high viability CD8+ cells. Here we report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.
authors.affiliation: Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Lewis
authors.affiliation: Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Evy
authors.initials: E
authors.lastname: de Leenheer
authors.affiliation: Laboratory of Immunology, MIGAL Galilee Research Institute, Kiryat Shmona 11016, Israel; Department of Biotechnology, Tel-Hai College, Upper Galilee 12210, Israel.
authors.forename: Sigal
authors.initials: S
authors.lastname: Fishman
authors.affiliation: Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
authors.forename: Lai Khai
authors.initials: LK
authors.lastname: Siew
authors.affiliation: Laboratory of Immunology, MIGAL Galilee Research Institute, Kiryat Shmona 11016, Israel; Department of Biotechnology, Tel-Hai College, Upper Galilee 12210, Israel.
authors.forename: Gideon
authors.initials: G
authors.lastname: Gross
authors.affiliation: Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK. Electronic address: wongfs@cardiff.ac.uk.
authors.forename: F Susan
authors.initials: FS
authors.lastname: Wong
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000637:transplantation*; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005434:Flow Cytometry; D016219:Immunotherapy, Adoptive / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD
keywords: Activation; CD8+ T cells; Immunotherapy