title: [Rapid PCR authentication Lonicera japanica].
abstract: To simply and rapid authenticate Lonicera japanica. Rapid allele-specific PCR primer was designed base on trnL-trnF 625 G/T Single nucleotide polymorphism and the PCR reaction systems including annealing temperature was optimized; optimized results were performed to authenticate L. japanica and its 9 adulterants. When 100 x SYBR Green I was added in the PCR product of 87 degrees C initial denatured 1 min; 87 degrees C denatured 5 s, 68 degrees C annealing 5 s, 30 cycle; L. japanica visualize strong green fluorescence under 365 nm UV lamp whereas adulterants without. The results indicate rapid allele-specific PCR could authenticate L. japanica and its adulterants rapidly and simply.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Hou
authors.forename: Lu-Qi
authors.initials: LQ
authors.lastname: Huang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000483:Alleles; D017931:DNA Primers / Q000235:genetics; D004340:Drug Contamination / Q000517:prevention & control; D031239:Lonicera / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control
chemical_list: D017931:DNA Primers