title: Evaluation of light-emitting diodes (LED) effect on skin biology (in vitro study).
abstract: BACKGROUND
  Interest in anti-aging approaches has grown significantly in recent years. The most popular are the non invasive methods to decrease the signs of aging. One such method is LED-based therapy.
  
  
  METHODS
  This study investigated the potential of two different wavelengths, 590 nm and 630 nm, combined or not, in the photobiomodulation of proteins involved in the slowdown of the skin aging.
  
  
  RESULTS
  These in vitro results on cell viability, cell shape, and mitochondrial function support and build on previous studies suggested that LED treatment is safe. Regarding its biological functions, our data indicated that the combination of two different wavelengths acted in synergy to enhance the impact of each irradiation alone. Combined, the LED wavelengths could improve in vitro the cell shape, the cell proliferation, and the level of major proteins involved in the healing process.
  
  
  CONCLUSIONS
  These benefits may lead to reinforcement of the skin organization and structure. This hypothesis will be checked in future clinical studies.
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: R
authors.initials: R
authors.lastname: Chabert
authors.affiliation: Dermatology Center, Nice, France.
authors.forename: L
authors.initials: L
authors.lastname: Fouque
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: S
authors.initials: S
authors.lastname: Pinacolo
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: N
authors.initials: N
authors.lastname: Garcia-Gimenez
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: M
authors.initials: M
authors.lastname: Bonnans
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: K
authors.initials: K
authors.lastname: Cucumel
authors.affiliation: Ashland Specialty Ingredients, Skin Research Center, Sophia Antipolis, France.
authors.forename: N
authors.initials: N
authors.lastname: Domloge
mesh_terms: D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000502:physiology* / Q000528:radiation effects; D008027:Light; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D011829:Radiation Dosage; D012666:Semiconductors; D012867:Skin / Q000166:cytology* / Q000528:radiation effects*; D012879:Skin Physiological Phenomena / Q000528:radiation effects*
keywords: chronological aging reverse; cumulative wavelengths; healing process; light-emitted diodes; proliferation