title: Molecular cloning and characterization of a β-1,3-glucan recognition protein from Plutella xylostella (L.).
abstract: The β-glucan recognition proteins (βGRPs) play a significant role as important pattern recognition proteins (PRPs) for recognizing conserved surface determinants of pathogens and trigger complex signaling pathways in invertebrates. In the present study, a full-length cDNA 1793bp encoding 479 amino acids and βGRP1 was obtained from Plutella xylostella by reverse transcription PCR (RT-PCR) (designed as P×βGRP1) which showed significant similarities with other insect's βGRPs. The transcription level was constitutively expressed and upregulated by microbial induction in all life stages of P. xylostella. Tissue distribution showed P×βGRP1 to be mainly expressed in fat body as detected by quantitative real-time PCR (qRT-PCR) and immunohistochemistry. Subsequent to knock down the P×βGRP1 expression and the transcripts of Toll-like receptor, cecropin 1 and cecropin 2 decreased in P. xylostella. Meanwhile, the bacterial colonies increased and the expression of four AMP genes decreased on injection of anti-P×βGRP1 into Bombyx mori. The results demonstrated that P×βGRP1 can play a vital role in response to the expression of AMP genes in P. xylostella.
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Huang
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan.
authors.forename: Shoaib
authors.initials: S
authors.lastname: Freed
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Ren
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China. Electronic address: jflbang@scau.edu.cn.
authors.forename: Fengliang
authors.initials: F
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006458:Hemolymph / Q000378:metabolism; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D007915:Lepidoptera / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D034622:RNA Interference; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014018:Tissue Distribution
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D011994:Recombinant Proteins; C405332:beta-1,3-glucan recognition protein, insect