title: Real-time electrochemical detection of hydrogen peroxide secretion in live cells by Pt nanoparticles decorated graphene-carbon nanotube hybrid paper electrode.
abstract: In this work, we develop a new type of flexible and lightweight electrode based on highly dense Pt nanoparticles decorated free-standing graphene-carbon nanotube (CNT) hybrid paper (Pt/graphene-CNT paper), and explore its practical application as flexible electrochemical biosensor for the real-time tracking hydrogen peroxide (H2O2) secretion by live cells. For the fabrication of flexible nanohybrid electrode, the incorporation of CNT in graphene paper not only improves the electrical conductivity and the mechanical strength of graphene paper, but also increases its surface roughness and provides more nucleation sites for metal nanoparticles. Ultrafine Pt nanoparticles are further decorated on graphene-CNT paper by well controlled sputter deposition method, which offers several advantages such as defined particle size and dispersion, high loading density and strong adhesion between the nanoparticles and the substrate. Consequently, the resultant flexible Pt/graphene-CNT paper electrode demonstrates a variety of desirable electrochemical properties including large electrochemical active surface area, excellent electrocatalytic activity, high stability and exceptional flexibility. When used for nonenzymatic detection of H2O2, Pt/graphene-CNT paper exhibits outstanding sensing performance such as high sensitivity, selectivity, stability and reproducibility. The sensitivity is 1.41 µA µM(-1) cm(-2) with a linear range up to 25 µM and a low detection limit of 10 nM (S/N=3), which enables the resultant biosensor for the real-time tracking H2O2 secretion by live cells macrophages.
authors.affiliation: School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China; School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Sun
authors.affiliation: School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China.
authors.forename: Kui
authors.initials: K
authors.lastname: He
authors.affiliation: School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China.
authors.forename: Zefen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Material Science and Engineering, Wuhan Institute of Technology, Wuhan, Hubei 430073, People's Republic of China. Electronic address: zhouaijun3@sina.com.
authors.forename: Aijun
authors.initials: A
authors.lastname: Zhou
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore. Electronic address: hduan@ntu.edu.sg.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Duan
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis* / Q000378:metabolism; D057230:Limit of Detection; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D037742:Nanotubes, Carbon; D010984:Platinum; D006108:Graphite; D006861:Hydrogen Peroxide
keywords: Cell tracking; Flexible electrochemical biosensor; Graphene–carbon nanotube hybrid paper; Nonenzymatic hydrogen peroxide detection; Pt nanoparticles; Sputter deposition