title: Evaluation of medicine effects on the interaction of myoglobin and its aptamer or antibody using atomic force microscopy.
abstract: The effects of medicine on the biomolecular interaction have been given increasing attention in biochemistry and affinity-based analytics since the environment in vivo is complex especially for the patients. Herein, myoglobin, a biomarker of acute myocardial infarction, was used as a model, and the medicine effects on the interactions of myoglobin/aptamer and myoglobin/antibody were systematically investigated using atomic force microscopy (AFM) for the first time. The results showed that the average binding force and the binding probability of myoglobin/aptamer almost remained unchanged after myoglobin-modified gold substrate was incubated with promazine, amoxicillin, aspirin, and sodium penicillin, respectively. These parameters were changed for myoglobin/antibody after the myoglobin-modified gold substrate was treated with these medicines. For promazine and amoxicillin, they resulted in the change of binding force distribution of myoglobin/antibody (i.e., from unimodal distribution to bimodal distribution) and the increase of binding probability; for aspirin, it only resulted in the change of the binding force distribution, and for sodium penicillin, it resulted in the increase of the average binding force and the binding probability. These results may be attributed to the different interaction modes and binding sites between myoglobin/aptamer and myoglobin/antibody, the different structures between aptamer and antibody, and the effects of medicines on the conformations of myoglobin. These findings could enrich our understanding of medicine effects on the interactions of aptamer and antibody to their target proteins. Moreover, this work will lay a good foundation for better research and extensive applications of biomolecular interaction, especially in the design of biosensors in complex systems.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha, Hunan 410082, P. R. China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Nandi
authors.initials: N
authors.lastname: Chen
authors.forename: Chenchen
authors.initials: C
authors.lastname: Zhou
authors.forename: Bianxia
authors.initials: B
authors.lastname: Luo
authors.forename: Shasha
authors.initials: S
authors.lastname: Du
mesh_terms: D000658:Amoxicillin / Q000737:chemistry / Q000494:pharmacology; D000906:Antibodies / Q000737:chemistry* / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D001241:Aspirin / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D015415:Biomarkers / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D018625:Microscopy, Atomic Force; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism; D010400:Penicillin G / Q000737:chemistry / Q000494:pharmacology; D011395:Promazine / Q000737:chemistry / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects
chemical_list: D000906:Antibodies; D052157:Aptamers, Nucleotide; D015415:Biomarkers; D009211:Myoglobin; D006046:Gold; D000658:Amoxicillin; D011395:Promazine; D010400:Penicillin G; D001241:Aspirin