title: An optimized protocol for isolating lymphoid stromal cells from the intestinal lamina propria.
abstract: Mesenchymal stromal cells in lymphoid organs, also called lymphoid stromal cells (LSCs), play a pivotal role in immunity by forming specialized microenvironments that provide signals for leukocyte migration, positioning, and survival. Best characterized in lymphoid organs, LSCs are also abundant in the intestinal mucosa, which harbors a rich repertoire of immune cells. However, the lack of efficient procedures for isolation and purification of LSCs from the intestine has been a major limitation to their characterization. Here we report a new method to efficiently isolate, in addition to immune cells, viable lymphoid stromal cells and other stromal subsets from the intestinal lamina propria for subsequent phenotypic and functional analysis.
authors.affiliation: Institut Pasteur, Lymphoid Tissue Development Unit, Paris, France.
authors.forename: Igor
authors.initials: I
authors.lastname: Stzepourginski
authors.affiliation: Institut Pasteur, Lymphoid Tissue Development Unit, Paris, France.
authors.forename: Gérard
authors.initials: G
authors.lastname: Eberl
authors.affiliation: Institut Pasteur, Lymphoid Tissue Development Unit, Paris, France. Electronic address: lucie.peduto@pasteur.fr.
authors.forename: Lucie
authors.initials: L
authors.lastname: Peduto
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C490441:Pdpn protein, mouse; D000098853:Podoplanin
keywords: Flow cytometry; Intestinal lymphoid stromal cells; Mucosal immunity; Stromal cell viability/yield; Tissue dissociation