title: Determination of acrolein in serum by high-performance liquid chromatography with fluorescence detection after pre-column fluorogenic derivatization using 1,2-diamino-4,5-dimethoxybenzene.
abstract: Acrolein is a major unsaturated aldehyde that is generated during the lipid peroxidation process. The measurement of acrolein in biological samples should be useful to estimate the degree of lipid peroxidation and to evaluate the effect of hazardous properties of acrolein on human health. In this study, a highly sensitive and selective high-performance liquid chromatography with fluorescence detection method was developed for the determination of acrolein in human serum. The proposed method involves the pre-column fluorogenic derivatization of acrolein with 1,2-diamino-4,5-dimethoxybenzene (DDB) as a reagent. The fluorescent derivative of acrolein could be detected clearly without any interfering reagent blank peaks because DDB does not have intrinsic fluorescence itself, and the detection limit was 10 nM (signal-to-noise ratio = 3). The proposed method could selectively detect acrolein in human serum with a simple protein precipitation treatment.
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.|Sasebo Chuo Hospital, Sasebo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Imazato
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Kanematsu
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Hino
authors.affiliation: Sasebo Chuo Hospital, Sasebo, Japan.
authors.forename: Yukitaka
authors.initials: Y
authors.lastname: Ueki
authors.affiliation: Showa University Fujigaoka Hospital, Yokohama, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Maehata
authors.affiliation: Department of Environmental and Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D000171:Acrolein / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D010655:Phenylenediamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D010655:Phenylenediamines; C047826:1,2-diamino-4,5-dimethoxybenzene; D000171:Acrolein
keywords: 1,2-diamino-4,5-dimethoxybenzene; acrolein; fluorescence detection; fluorogenic derivatization; high-performance liquid chromatography; lipid peroxidation