title: The C1473G polymorphism in the Tryptophan hydroxylase-2 gene: involvement in ethanol-related behavior in mice.
abstract: Tryptophan hydroxylase-2 (Tph2) is the rate limiting enzyme of serotonin synthesis in the brain. The functional (C1473G) polymorphism in the mouse Tph2 gene affecting the enzymatic activity was suspected to be involved in behavioral actions of ethanol (EtOH). Congenic B6-1473C (C/C) and B6-1473G (G/G) lines bred from C57BL/6 mice were not different in EtOH-induced sleep time and hypothermia. B6-1473C mice displayed increased EtOH preference on the second and third days compared to that of the first day, but no differences in this parameter was found across genotypes. Both lines demonstrated the same responsiveness to hypothermic and hypnotic effect of acute EtOH treatment after repeated alcohol exposure. However, acute EtOH administration led to reduction of locomotor activity in B6-1473C, but not in B6-1473G animals and to increase of time spent in the center of open-field arena in B6-1473G, but not in B6-1473C mice. Thus, the present study indicates the involvement of C1473G polymorphism in mTph2 gene in the regulation of EtOH-induced effects on locomotor activity and anxiety-like behavior in mice.
authors.affiliation: Laboratory of Behavioral Neurogenomics, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk 630090, Russia. Electronic address: daryabazovkina@gmail.com.
authors.forename: Darya V
authors.initials: DV
authors.lastname: Bazovkina
authors.affiliation: Laboratory of Population Ethnogenetics, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk 630090, Russia. Electronic address: daria_lichman@bionet.nsc.ru.
authors.forename: Daria V
authors.initials: DV
authors.lastname: Lichman
authors.affiliation: Center for Genetic Resources of Laboratory Animals, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Avenue, Novosibirsk 630090, Russia. Electronic address: akulikov@ngs.ru.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Kulikov
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D003433:Crosses, Genetic; D000431:Ethanol / Q000494:pharmacology*; D005838:Genotype; D008124:Locomotion / Q000187:drug effects*; D008297:Male; D008810:Mice, Inbred C57BL; D011110:Polymorphism, Genetic; D012890:Sleep / Q000187:drug effects*; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol; C487802:Tph2 protein, mouse; D014365:Tryptophan Hydroxylase
keywords: C1473G polymorphism; Ethanol-related behavior; Mice; Tryptophan hydroxylase-2 gene