title: In vitro activity of 23 tea extractions and epigallocatechin gallate against Candida species.
abstract: In this study, we investigate the susceptibility of Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis, and Aspergillus fumigatus using the EUCAST microdilution minimum inhibitory concentration (MIC) method (final tea supernatant concentration range 5.0-0.005 mg/ml) to 23 different teas and tea catechins including epigallocatechin gallate (EGCG) isolated from green tea. All teas exhibited potent in vitro antifungal activity against C. glabrata. Six out of nine green teas and three of eight black teas had an MIC of 0.078 mg/ml, one white tea had an MIC of 0.156 mg/ml, and finally three of five oolong teas had an MIC of 0.156 mg/ml. Three teas exhibited activity against C. albicans (MIC 1.25 mg/ml), one green tea was active against C. parapsilosis (MIC 1.25 mg/ml), but none were effective against C. krusei, C. tropicalis or A. fumigatus at the concentrations tested. The MIC of EGCG was 0.3125 μg/ml against C. glabrata and 5.0 μg/ml against C. albicans and C. parapsilosis. The effect was fungicidal against C. glabrata at higher concentrations. In conclusion, EGCG and other yet undefined substances in tea have differential antifungal activity in vitro against C. glabrata, C. albicans and C. parapsilosis. These data indicate that components of tea and EGCG might be useful particularly for the treatment of C. glabrata infections and warrants further investigations.
authors.affiliation: Department of Clinical Microbiology, Rigshospitalet Unit of Mycology and Parasitology, Statens Serum Institut, Copenhagen Denmark ming.chen@rsyd.dk.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Clinical Microbiology, Rigshospitalet.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Unit of Mycology and Parasitology, Statens Serum Institut, Copenhagen Denmark.
authors.forename: Maiken Cavling
authors.initials: MC
authors.lastname: Arendrup
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001232:Aspergillus fumigatus / Q000187:drug effects; D028241:Camellia sinensis / Q000737:chemistry*; D002175:Candida / Q000187:drug effects*; D002392:Catechin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000935:Antifungal Agents; D010936:Plant Extracts; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Aspergillus; Candida; antifungal activity; epigallocatechin gallate (EGCG); tea