title: Switch regulation of interleukin-1 beta in downstream of inflammatory cytokines induced by two micro-sized silica particles on differentiated THP-1 macrophages.
abstract: To investigate the regulated role of IL-1β in initiating and maintaining inflammation, PMA-differentiated THP-1 macrophages were exposed to two micro-sized crystalline silica particles (Si3-5μm and Si1μm) from 3h to 24h, respectively. Cytotoxicity and inflammatory cytokines (IL-1β, TNF-α and IL-6) expressions measured showed that they were induced by both silica particles in positive dose-dependent manners. The levels of inflammatory cytokines induced by Si1μm were higher than those induced by Si3-5μm at low concentration. When pretreated with anti-human IL-1β, not only the high levels of IL-1β but also elevated TNF-α and IL-6 induced by both silica particles were remarkably blocked, especially Si1μm particle. In addition, recombinant human IL-1β protein could induce macrophages to strikingly augment TNF-α and IL-6 expressions. Our data suggest that IL-1β could play a critical role of switching regulation in the downstream inflammation induced by micro-sized silica particles.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; School of Public Health, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Xiuqing
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Jiali
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Rong
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China. Electronic address: wchen@mails.tjmu.edu.cn.
authors.forename: Weihong
authors.initials: W
authors.lastname: Chen
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D000906:Antibodies; D016207:Cytokines; D012822:Silicon Dioxide
keywords: IL-1β; Inflammatory cytokines; Macrophages; Silica particles