title: [Non-modified magnetic beads coupled with multiple real-time PCR for detection and quantification of mycotoxigenic fungi in paprika samples].
abstract: OBJECTIVE
  To establish a method for detecting 3 common toxigenic molds (Aspergillus, Penicillium, and Fusarium) based on non-modified magnetic beads coupled with multiple real-time PCR (NMB-multiple qPCR).
  
  
  METHODS
  The primers and genus-specific probes were designed based on the rDNA sequences to develop a multiple real-time PCR using non-modified magnetic bead to enrichment of fungal spores. The sensitivity, specificity and repeatability of this assay were evaluated.
  
  
  RESULTS
  The detection limit of this assay for spiked samples was 10(4) CFU/g, demonstrating a 10-fold greater detection sensitivity of this assay than that of real-time PCR. The NMB-multiple qPCR assay also showed good specificity and reproducibility and yielded comparable results with those by traditional colony counting method for spiked samples (P>0.05).
  
  
  CONCLUSIONS
  NMB-multiple qPCR assay we established allows rapid and sensitive detection of common mycotoxigenic fungi in paprika.
authors.affiliation: Department of Public Health Laboratory Sciences, West China School of Public Health, Sichuan University, Chengdu 610041, China.E-mail: edrollanca@163.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Su-Yuan
authors.initials: SY
authors.lastname: Wang
authors.forename: Zheng-Mao
authors.initials: ZM
authors.lastname: Ye
authors.forename: Li-Shi
authors.initials: LS
authors.lastname: Zhang
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Pei
mesh_terms: D001230:Aspergillus; D002212:Capsicum / Q000382:microbiology*; D017931:DNA Primers; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D005658:Fungi / Q000302:isolation & purification*; D005670:Fusarium; D060328:Magnetic Phenomena; D010407:Penicillium; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers