title: Enhancing Bidirectional Electron Transfer of Shewanella oneidensis by a Synthetic Flavin Pathway.
abstract: Flavins regulate the rate and direction of extracellular electron transfer (EET) in Shewanella oneidensis. However, low concentration of endogenously secreted flavins by the wild-type S. oneidensis MR-1 limits its EET efficiency in bioelectrochemical systems (BES). Herein, a synthetic flavin biosynthesis pathway from Bacillus subtilis was heterologously expressed in S. oneidensis MR-1, resulting in ∼25.7 times' increase in secreted flavin concentration. This synthetic flavin module enabled enhanced bidirectional EET rate of MR-1, in which its maximum power output in microbial fuel cells increased ∼13.2 times (from 16.4 to 233.0 mW/m(2)), and the inward current increased ∼15.5 times (from 15.5 to 255.3 μA/cm(2)).
authors.affiliation: ‡School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.|§Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.affiliation: §Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Yuanzhao
authors.initials: Y
authors.lastname: Ding
authors.affiliation: ‡School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457, Singapore.|§Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Yidan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: §Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.|∥School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 637798, Singapore.
authors.forename: Bin
authors.initials: B
authors.lastname: Cao
authors.affiliation: §Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Scott A
authors.initials: SA
authors.lastname: Rice
authors.affiliation: §Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.
authors.forename: Staffan
authors.initials: S
authors.lastname: Kjelleberg
authors.affiliation: †Key Laboratory of Systems Bioengineering (Ministry of Education), SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P.R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Song
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001674:Bioelectric Energy Sources; D055664:Electrochemical Techniques; D004579:Electron Transport; D005415:Flavins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D020592:Shewanella / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D005415:Flavins
keywords: Shewanella oneidensis MR-1; cofactor; electron shuttle; extracellular electron transfer; flavin; synthetic biology