title: Characterization of two thymosins as immune-related genes in common carp (Cyprinus carpio L.).
abstract: Prothymosin alpha (ProTα) and thymosin beta (Tβ) belong to thymosin family, which consists of a series of highly conserved peptides involved in stimulating immune responses. ProTα b and Tβ are still poorly studied in teleost. Here, the full-length cDNAs of ProTα b and Tβ-like (Tβ-l) were cloned and identified in common carp (Cyprinus carpio L.). The expressions of carp ProTα b and Tβ-l exhibited rise-fall pattern and then trended to be stable during early development. After spring viraemia of carp virus (SVCV) infection, the carp ProTα b and Tβ-l transcripts were significantly up-regulated in some immune-related organs. When transiently over-expressed carp ProTα b and Tβ-l in zebrafish, these two proteins up-regulated the expressions of T lymphocytes-related genes (Rag 1, TCR-γ, CD4 and CD8α). These results suggest that carp ProTα b and Tβ may ultimately enhance the immune response during viral infection and modulate the development of T lymphocytes in teleost.
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong. Electronic address: xzg555898@hotmail.com.
authors.forename: Zhangang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Feng
authors.affiliation: Key Laboratory of Aquatic Animal Diseases, Shenzhen Exit & Entry Inspection and Quarantine Bureau, Shenzhen 518001, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: huangrong@ihb.ac.cn.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
authors.forename: Qionglin
authors.initials: Q
authors.lastname: Guo
mesh_terms: D000818:Animals; D002347:Carps / Q000235:genetics* / Q000276:immunology; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology; D011498:Protein Precursors / Q000235:genetics* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013947:Thymosin / Q000031:analogs & derivatives* / Q000235:genetics / Q000276:immunology; D014452:Ubiquitins / Q000235:genetics* / Q000276:immunology; D015027:Zebrafish / Q000235:genetics
chemical_list: D029941:Fish Proteins; D011498:Protein Precursors; D014452:Ubiquitins; C040782:prothymosin alpha; C047192:thymosin beta(1); D013947:Thymosin
keywords: Common carp; Spring viraemia of carp virus (SVCV); beta thymosin (Tβ); prothymosin alpha (ProTα)