title: Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds.
abstract: The broad application of electrospun nanofibrous scaffolds in tissue engineering is limited by their small pore size, which has a negative influence on cell migration. This disadvantage could be significantly improved through the combination of nano- and microfibrous structure. To accomplish this, different nano/microfibrous scaffolds were produced by hybrid electrospinning, combining solution electrospinning with melt electrospinning, while varying the content of the nanofiber. The morphology of the silk fibroin (SF)/poly(ε-caprolactone) (PCL) nano/microfibrous composite scaffolds was investigated with field-emission scanning electron microscopy, while the mechanical and pore properties were assessed by measurement of tensile strength and mercury porosimetry. To assay cell proliferation, cell viability, and infiltration ability, human mesenchymal stem cells were seeded on the SF/PCL nano/microfibrous composite scaffolds. From in vivo tests, it was found that the bone-regenerating ability of SF/PCL nano/microfibrous composite scaffolds was closely associated with the nanofiber content in the composite scaffolds. In conclusion, this approach of controlling the nanofiber content in SF/PCL nano/microfibrous composite scaffolds could be useful in the design of novel scaffolds for tissue engineering.
authors.affiliation: Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea ; Bone Cell Biotech, Daejeon, South Korea.
authors.forename: Beom Su
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, South Korea ; Central Research Institute, Humedix, Anyang, South Korea.
authors.forename: Ko Eun
authors.initials: KE
authors.lastname: Park
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, South Korea.
authors.forename: Min Hee
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Periodontology, School of Dentistry, Wonkwang University, Iksan, South Korea.
authors.forename: Hyung Keun
authors.initials: HK
authors.lastname: You
authors.affiliation: Wonkwang Bone Regeneration Institute, Wonkwang University, Iksan, South Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Advanced Organic Materials and Textile System Engineering, Chungnam National University, Daejeon, South Korea.
authors.forename: Won Ho
authors.initials: WH
authors.lastname: Park
mesh_terms: D001861:Bone Regeneration / Q000187:drug effects*; D002478:Cells, Cultured; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011091:Polyesters; C016240:polycaprolactone; D005349:Fibroins
keywords: bone regeneration; composite scaffolds; microfibers; nanofibers; poly(ε-caprolactone) (PCL); silk fibroin (SF)