title: Association between CYP1B1 gene polymorphisms and risk factors and susceptibility to laryngeal cancer.
abstract: BACKGROUND
  The aim of this study was to investigate the association between polymorphism of the cytochrome P450 1B1 (CYP1B1) gene, a metabolic enzyme gene, and the susceptibility to laryngeal cancer among the Chinese Han population.
  
  
  METHODS
  In a case-control study, we investigated polymorphisms in the CYP1B1 gene (rs10012, rs1056827, and rs1056836) with a real-time quantitative polymerase chain reaction (PCR) assay (TaqMan). The study was conducted with 300 Chinese Han patients with laryngeal cancer and 300 healthy Chinese Han subjects in a control group. We also studied the interactions between genetic polymorphism and risk factors such as smoking and alcohol consumption in the pathogenesis of laryngeal cancer.
  
  
  RESULTS
  There were statistically significant differences in the distributions of the rs1056827 and rs1056836 genotypes between the 2 groups. Regarding rs1056827, carriers of the T allele had a significantly higher risk of laryngeal cancer than the G-allele carriers (OR=1.4339, 95% CI: 1.1268-1.8247; P=0.0034). The difference was still statistically significant after adjusting for factors such as age, sex, smoking, and drinking (adjusted OR=1.743, 95% CI: 1.124-3.743, P<0.001). However, regarding rs1056836, the G allele carriers had a significantly lower risk of laryngeal cancer than the C allele carriers (OR=0.5557, 95% CI: 0.3787-0.8154; P=0.0027). The difference was statistically significant even after adjusting for factors such as age, sex, smoking, and drinking (adjusted OR=0.5641, 95% CI: 0.3212-0.8121, P=0.001). Subjects who carry the C-T-C haplotype have a significantly increased incidence of laryngeal cancer. We also found that CYP1B1 rs1056827 polymorphism had synergistic effects with smoking or alcohol consumption regarding the risk of laryngeal cancer.
  
  
  CONCLUSIONS
  CYP1B1 gene polymorphism is closely related to the onset of laryngeal cancer. There is a mutually synergistic effect between smoking, alcohol consumption, and CYP1B1 gene polymorphisms regarding laryngeal cancer.
authors.affiliation: Department of Otolaryngology, Jiangsu Province Academy of Traditional Chinese Medicine, Kunshan, Jiangsu, China (mainland).
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Yu
authors.affiliation: Operation Room, Jiangsu Province Academy of Traditional Chinese Medicine, Kunshan, Jiangsu, China (mainland).
authors.forename: Wei-Guan
authors.initials: WG
authors.lastname: Chen
authors.affiliation: Department of Tumor Surgical, Jiangsu Province Academy of Traditional Chinese Medicine, Kunshan, Jiangsu, China (mainland).
authors.forename: Quan-Lin
authors.initials: QL
authors.lastname: Feng
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Jiangsu Province Academy of Traditional Chinese Medicine, Kunshan, Jiangsu, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, Jiangsu Province Academy of Traditional Chinese Medicine, Kunshan, Jiangsu, China (mainland).
authors.forename: Man-Jie
authors.initials: MJ
authors.lastname: Jiang
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, China Jiangsu Province Traditional Chinese Medicine Hospital, Nanjing, Jiangsu, China (mainland).
authors.forename: Ze-Qing
authors.initials: ZQ
authors.lastname: Li
mesh_terms: D000368:Aged; D000428:Alcohol Drinking; D000483:Alleles; D016022:Case-Control Studies; D002681:China; D065633:Cytochrome P-450 CYP1B1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007822:Laryngeal Neoplasms / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D011110:Polymorphism, Genetic; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects
chemical_list: C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1