title: Genetic polymorphism of glutathione S-transferase P1 (GSTP1) in Delhi population and comparison with other global populations.
abstract: Glutathione S-transferases (GSTs) belong to a super family of phase II detoxification enzymes, which play an important role in protecting cells from damage caused by endogenous and exogenous compounds by conjugating reactive intermediates with glutathione to produce less reactive water-soluble compounds. In the present study, we determined the frequencies of two polymorphisms in exon 5 and exon 6 of GSTP1 gene in 500 normal individuals from Delhi. GSTP1 polymorphism was analysed by PCR-RFLP using amplification refractory mutation system (ARMS) assay. Two polymorphic sites in GSTP1 (Ile105 → Val105; Ala114 → Val114) have been analysed simultaneously, which results in four alleles: GSTP1*A (wild-type Ile105; Ala114), GSTP1*B (Val105; Ala114), GSTP1*C (Val105; Val114) and GSTP1*D (Ile105; Val114). The GSTP1 allele frequency in Delhi population was 0.663, 0.248, 0.069, and 0.020 for GSTP1*A, GSTP1*B, GSTP1*C, and GSTP1*D respectively. The frequency of Ile105 and Val105 allele was 0.683 and 0.317 respectively and it was calculated for the purpose of comparison with published data where all the four alleles were not analysed. GSTP1 alleles from Delhi population were compared with reported frequencies from all over India, and from other ethnic groups worldwide. This study would provide a basic database for future genetic studies.
authors.affiliation: Division of Molecular Diagnostics, Institute of Cytology and Preventive Oncology (ICMR), I-7, sector 39, NOIDA-201301, India.
authors.forename: Anita
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Centre for Biotechnology, University of Allahabad, Allahabad- 211001, India ; Division of Molecular Oncology, Institute of Cytology and Preventive Oncology (ICMR), I-7, NOIDA, India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Pandey
authors.affiliation: Division of Epidemiology and Biostatistics, India.
authors.forename: Shashi
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Department of Life Science, Central University of Tamilnadu, Thiruvarur-610101, India.
authors.forename: Indranil
authors.initials: I
authors.lastname: Chatterjee
authors.affiliation: Institute of Cytology and Preventive Oncology (ICMR), I-7, Sector 39, NOIDA-201301, Uttar Pradesh, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Mehrotra
authors.affiliation: Division of Epidemiology and Biostatistics, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Sehgal
authors.affiliation: Division of Molecular Diagnostics, Institute of Cytology and Preventive Oncology (ICMR), I-7, sector 39, NOIDA-201301, India.
authors.forename: Joginder K
authors.initials: JK
authors.lastname: Sharma
keywords: ARMS assay; GST polymorphism; GSTP1; Glutathione S-transferase; North Indians