title: The bioactive composite film prepared from bacterial cellulose and modified by hydrolyzed gelatin peptide.
abstract: The hydrolyzed gelatin peptides, obtained from the hydrolysis of Tilapia nilotica skin gelatin with alcalase and pronase E, were fractionated using an ultrafiltration system into hydrolyzed gelatin peptides-a (10 kDa membrane), hydrolyzed gelatin peptides-b1, and hydrolyzed gelatin peptides-b2 (5 kDa membrane) fractions. The highest oxygen radical absorbance capacity was observed in hydrolyzed gelatin peptides-b2, which contained more nonpolar amino acids than the other hydrolyzed gelatin peptides. Hydrolyzed gelatin peptides-b2 at a concentration of 12.5 mg/ml exhibited the highest proliferation ability and increased the expression of Type I procollagen mRNA, which indicated an enhanced collagen synthesis. Hydrolyzed gelatin peptides protected Detroit 551 cells from 2,2'-azobis(2-amidinopropane) dihydrochloride-induced oxidative damage and increased cell viability. Hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm were less eligible for Detroit 551 cell proliferation than bacterial cellulose. The release of hydrolyzed gelatin peptides in bacterial cellulose film was slower than that in hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm; thus, bacterial cellulose film and hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm are suitable candidates for applications in delayed release type and rapid release type biofilms, respectively.
authors.affiliation: Department of Food Science, National Ilan University, Ilan City, Taiwan, R.O.C.
authors.forename: Shih-Bin
authors.initials: SB
authors.lastname: Lin
authors.affiliation: Department of Food Science, National Ilan University, Ilan City, Taiwan, R.O.C.
authors.forename: Chia-Che
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Food Science, National Ilan University, Ilan City, Taiwan, R.O.C.
authors.forename: Li-Chen
authors.initials: LC
authors.lastname: Chen
authors.affiliation: Department of Food Science, National Ilan University, Ilan City, Taiwan, R.O.C hhchen@niu.edu.tw.
authors.forename: Hui-Huang
authors.initials: HH
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001684:Biological Dressings; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D002482:Cellulose / Q000737:chemistry*; D023681:Cichlids; D024042:Collagen Type I / Q000235:genetics; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010455:Peptides / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D010455:Peptides; D012333:RNA, Messenger; D005780:Gelatin; D002482:Cellulose
keywords: Hydrolyzed gelatin peptide; antioxidant; bacterial cellulose; cell bioactivity; fibroblast