title: Assembly-line manipulation of droplets in microfluidic platform for fluorescence encoding and simultaneous multiplexed DNA detection.
abstract: In this article, a new mode of droplets manipulation is presented and applied for simultaneous multiplexed DNA detection. We call this droplets manipulation, "assembly-line manipulation of droplets (ALMD)". Firstly, multiple droplets containing the same target mixtures are generated in the microchannel, and then fused with later generated different droplets containing corresponding probes, respectively. Finally, all the fused droplets were fluorescence imaged on-line and real-time. The successful implementation of droplets fluorescence encoding based on ALMD shows the reproducibility and accuracy of this manipulation mode. As a proof-of-concept application, the simultaneous multiplexed DNA detection was carried out through the model of human immunodeficiency virus (HIV) gene sequence and variola virus (small pox, VV) gene sequence based on ALMD in the microfluidic system. It is proved that this method achieves simultaneous multiplexed DNA measurements with a significantly time-saving way and without different dye-labelled probes or complex operation procedures. In addition, it reveals the possibility of high-throughput biosensing with simple chip design and detection equipment.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Jinyang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Tai
authors.initials: T
authors.lastname: Ye
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Xia
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
authors.forename: Xinghu
authors.initials: X
authors.lastname: Ji
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China. Electronic address: zhkhe@whu.edu.cn.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
mesh_terms: D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D004867:Equipment Design; D005453:Fluorescence; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D009693:Nucleic Acid Hybridization; D045663:Quantum Dots / Q000737:chemistry; D015203:Reproducibility of Results; D012901:Variola virus / Q000235:genetics*
chemical_list: D015342:DNA Probes; D004279:DNA, Viral
keywords: Assembly-line manipulation; Encoding; Microdroplet; Quantum dots; Simultaneous detection