title: A novel modulation of structural and functional changes of mouse bone marrow derived dendritic cells (BMDCs) by interleukin-2(IL-2).
abstract: IL-2 is a pleiotropic cytokine produced by T cell after antigen activation of T cell and it is so called T cell growth factor. A large number of documents suggest that Il-2 plays pivotal roles in the immune response and now Il-2 is an approved drug being used for various kinds of diseases such as cancer and dermatitis. (1) The aim of present exploration was to look at effect of IL-2 on structural, phenotypic and functional maturation of murine BMDCs. The structural and phenotypic maturation of BMDCs under influence of IL-2 were evaluated by light microscope and flow cytometry (FCM). The functional maturation of BMDCs was confirmed by cytochemistry assay, FITC-dextran, acid phosphatase (ACP) activity, bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IL-2 up-regulated the expression of key surface markers such as: CD80, CD83, CD86, CD40 and MHC II molecules on BMDCs, down-regulated phagocytosis activity, induced more production of IL-12 and TNF-α secreted by BMDCs. Therefore it can be concluded that IL-2 effectively enhance the maturation of BMDCs. Our results provide direct evidence to support IL-2 would be used as a potent adjuvant in preparation of DC-based vaccines, as well as an immune remedy for cancer situation.
authors.affiliation: a Department of Clinical Detection ; General Hospital of Shenyang Military Command ; Shenyang , China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Yiming
authors.initials: Y
authors.lastname: Meng
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Hou
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects*; D003584:Cytological Techniques; D003713:Dendritic Cells / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology*; D005260:Female; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D018664:Interleukin-12 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D008853:Microscopy; D010587:Phagocytosis / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12
keywords: ACP, Acidic phosphatase; APCs, LPS; BMDCs, Bone marrow derived dendritic cells; Bone marrow derived dendritic cells; CTL, Cytotoxic lymphocyte; DAB 3 3′-diaminobenzidine; FCM, Fow cytometry; IL-2, Interlukin-2; Interlukin-2; Lipopolysaccharide; MACS, Magnetic activated cell sorting; MTT 3-(4 5-Dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide; TEM, Transmission electron microscopy; maturation; surface molecules