title: Registered report: senescence surveillance of pre-malignant hepatocytes limits liver cancer development.
abstract: The Kang et al. (2011), published in Nature in 2011. The experiments that will be replicated are those reported in Figures 3B, 3C, 3E, and 4A. In these experiments, Kang et al. (2011) demonstrate the phenomenon of oncogene-induced cellular senescence and immune-mediated clearance of senescent cells after intrahepatic injection of NRAS (Figures 2I, 3B, 3C, and 3E). Additionally, Kang et al. (2011) show the specific necessity of CD4+ T cells for immunoclearance of senescent cells (Figure 4A). The Reproducibility Project: Cancer Biology is a collaboration between the eLife.
authors.affiliation: Mouse Biology Program, University of California-Davis, Davis, California.
authors.forename: Samrrah
authors.initials: S
authors.lastname: Raouf
authors.affiliation: Reveal Biosciences, San Diego, California.
authors.forename: Claire
authors.initials: C
authors.lastname: Weston
authors.affiliation: Stanford University, Stanford, California.
authors.forename: Nora
authors.initials: N
authors.lastname: Yucel
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D063646:Carcinogenesis / Q000473:pathology*; D016922:Cellular Senescence; D005260:Female; D022781:Hepatocytes / Q000473:pathology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D008810:Mice, Inbred C57BL; D015203:Reproducibility of Results
keywords: CD4+ T-cells; Reproducibility Project: Cancer Biology; cell biology; human biology; immune-mediated clearance; medicine; methodology; mouse; oncogene-induced senescence