title: Investigation of the population structure of Mycobacterium abscessus complex strains using 17-locus variable number tandem repeat typing and the further distinction of Mycobacterium massiliense hsp65 genotypes.
abstract: Mycobacterium abscessus complex is a significant pathogen in patients with non-cystic fibrosis (non-CF). Nevertheless, there is little description of the genetic diversity of this species. The aims of this study were to investigate the distribution of M. abscessus complex isolated from respiratory specimens by variable number tandem repeat (VNTR) typing. The results of 104 clinical isolates from 104 non-CF patients were compared using PFGE, hsp65 genotypes and clarithromycin susceptibility. The allelic diversity (Hunter-Gaston Discriminatory Index) of the 17 loci examined by VNTR typing was high (0.977). We determined that C28 sequevar erm(41) genotypes and clarithromycin-acquired resistance isolates were scattered in the minimum spanning tree. Intriguingly, VNTR typing and PFGE were highly congruent and revealed that there were clear examples of grouping of isolates from different individuals amongst both M. abscessus and M. massiliense, and showed five clusters of distinct identical isolates. Within these clusters, M. massiliense hsp65 type I formed three different clusters. Although the distribution of M. massiliense hsp65 type II-1 was low (9.3 %), M. massiliense hsp65 type II-1 isolates separated from clusters contained hsp65 type I isolates. Thus, M. massiliense hsp65 genotypes could be discriminated by analysing VNTRs with sufficient genetic distance for intra-species-level discrimination.
authors.affiliation: Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku Sakai, Osaka 591-8555, Japan.
authors.forename: Shiomi
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: Department of Microbiology, Kobe Institute of Health, 4-6 Minatojima-nakamachi, Chuo-ku Kobe, Hyogo 650-0046, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Arikawa
authors.affiliation: Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku Sakai, Osaka 591-8555, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Tsuyuguchi
authors.affiliation: Clinical Research Advisor, Japan Anti-Tuberculosis Association, Fukujuji Hospital, 3-1-24 Matsuyama, Kiyose, Tokyo 204-0022, Japan.
authors.forename: Atsuyuki
authors.initials: A
authors.lastname: Kurashima
authors.affiliation: Center for Respiratory Diseases, Japan Community Health Care Organization Hokkaido Hospital, 8-3-18 Ichijyo, Nakanoshima, Toyohira-ku Sapporo, Hokkaido 062-8618, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Harada
authors.affiliation: Center for Respiratory Diseases, National Hospital Organization Tokyo National Hospital, 3-1-2 Takeoka, Kiyose, Tokyo 204-8585, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagai
authors.affiliation: Internal Medicine, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku Sakai, Osaka 591-8555, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Department of Microbiology, Kobe Institute of Health, 4-6 Minatojima-nakamachi, Chuo-ku Kobe, Hyogo 650-0046, Japan.
authors.forename: Tomotada
authors.initials: T
authors.lastname: Iwamoto
authors.affiliation: Internal Medicine, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku Sakai, Osaka 591-8555, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Hayashi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D018834:Chaperonin 60 / Q000235:genetics*; D017291:Clarithromycin / Q000494:pharmacology; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D018598:Minisatellite Repeats / Q000235:genetics*; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000382:microbiology; D017422:Sequence Analysis, DNA
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004269:DNA, Bacterial; C074119:heat-shock protein 65, Mycobacterium; D017291:Clarithromycin