title: Non-destructive detection of fish spoilage using a wireless basic volatile sensor.
abstract: A hydrogel-pH-electrode based near-field passive volatile sensor is described for real-time monitoring of fish spoilage. The sensor employs a varactor-based LC resonator that can be interrogated remotely using inductive coupling. The sensor's resonant frequency varies in response to the basic volatile spoilage compounds (total volatile basic nitrogen, TVB-N) in the headspace of packaged fish. The sensor is shown to have a linear response to logarithm of the ammonia gas concentration with a detection limit of 0.001 mg L(-1) (1.5 ppm). Trials on tilapia at 24 °C and 4 °C, employing direct comparison of sensor measurements with microbial analysis, indicate that the sensor response is correlated with the bacterial growth pattern in fish samples. It is shown that the sensor can distinctly identify when the product rejection level (10(7) cfu g(-1) bacterial population) occurs for both 24 °C and 4 °C storage conditions. This demonstrates a potential for real-time monitoring of fish spoilage. The wireless sensor is suited to embedding in packaging material and does not require an integrated circuit, making it amenable to inexpensive mass production using printed electronic technology.
authors.affiliation: Department of Electrical and Computer Engineering, University of Manitoba, 75 Chancellor's Circle, Winnipeg, MB, Canada R3T 5V6.
authors.forename: Sharmistha
authors.initials: S
authors.lastname: Bhadra
authors.affiliation: Department of Food Science, University of Manitoba, 13 Freedman Crescent, Winnipeg, MB, Canada R3T 2N2.
authors.forename: Claudia
authors.initials: C
authors.lastname: Narvaez
authors.affiliation: Department of Electrical and Computer Engineering, University of Manitoba, 75 Chancellor's Circle, Winnipeg, MB, Canada R3T 5V6.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Thomson
authors.affiliation: Department of Electrical and Computer Engineering, University of Manitoba, 75 Chancellor's Circle, Winnipeg, MB, Canada R3T 5V6. Electronic address: Gregory.Bridges@umanitoba.ca.
authors.forename: Greg E
authors.initials: GE
authors.lastname: Bridges
mesh_terms: D000641:Ammonia / Q000737:chemistry; D000818:Animals; D058491:Bacterial Load; D004566:Electrodes; D005399:Fishes / Q000382:microbiology*; D005513:Food Inspection / Q000379:methods*; D005516:Food Microbiology / Q000379:methods*; D020100:Hydrogels / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry; D011549:Pseudomonas / Q000302:isolation & purification; D012834:Silver / Q000737:chemistry; D018030:Silver Compounds / Q000737:chemistry
chemical_list: D020100:Hydrogels; D018030:Silver Compounds; D012834:Silver; D000641:Ammonia; C037548:silver chloride; D009584:Nitrogen
keywords: Ammonia vapor; Fish spoilage; Food freshness; Inductive coupling; LC resonator; Passive RFID; TVB-N; Volatiles; Wireless sensor; pH-electrode