title: Alteration or adaptation, the two roads for human gastric mucin glycosylation infected by Helicobacter pylori.
abstract: Helicobacter pylori is a Gram-negative bacterium that colonizes the mucus niche of the gastric mucosa and infects more than half of the world's human population. Chronic infection may cause gastritis, duodenal ulcer, intestinal metaplasia or gastric cancer. In the stomach, H. pylori interacts with O-glycans of gastric mucins but the mechanism by which the bacteria succeed in altering the mucosa remains mainly unknown. To better understand the physiopathology of the infection, inhibitory adhesion assays were performed with various O-glycans expressed by human gastric mucins, and topographic expression of gastric mucins MUC5AC and MUC6 was analyzed for healthy uninfected individuals, for infected asymptomatic individuals and for patients infected by H. pylori and having the incomplete type of intestinal metaplasia. The glycosylation of the gastric mucosa of asymptomatic individuals infected by H. pylori was determined and compared with the glycosylation pattern found for patients with the incomplete type of intestinal metaplasia. Results show that H. pylori manages to modulate host's glycosylation during the course of infection in order to create a favorable niche, whereas asymptomatic infected individuals seem to counteract further steps of infection development by adapting their mucus glycosylation.
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Joncquel Chevalier Curt
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Lecointe
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France UCLille, Lille F-59000, France Groupe Hospitalier de l'Institut Catholique Lillois/ Faculté Libre de Médecine, Service d'Anatomie Pathologie, Lille F-59000, France.
authors.forename: Adriana
authors.initials: A
authors.lastname: Mihalache
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Rossez
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France UCLille, Lille F-59000, France Groupe Hospitalier de l'Institut Catholique Lillois/ Faculté Libre de Médecine, Service d'Anatomie Pathologie, Lille F-59000, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Gosset
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France.
authors.forename: Renaud
authors.initials: R
authors.lastname: Léonard
authors.affiliation: Univ Lille Nord de France, Lille F-59000, France USTL, UGSF, IFR 147, Villeneuve d'Ascq F-59650, France CNRS, UMR 8576, Villeneuve d'Ascq F-59650, France catherine.robbe@univ-lille1.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Robbe-Masselot
mesh_terms: D005752:Gastric Mucins / Q000378:metabolism*; D006031:Glycosylation; D016481:Helicobacter Infections / Q000378:metabolism* / Q000382:microbiology; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans
chemical_list: D005752:Gastric Mucins
keywords: Helicobacter pylori; asymptomatic patients; glycosylation; human gastric mucins; intestinal metaplasia