title: Genes for biosynthesis of butenolide-like signalling molecules inStreptomyces ghanaensis, their role in moenomycin production.
abstract: Moenomycins (Mm) - phosphoglycolipid compounds produced byStreptomyces ghanaensisATCC14672 - are considered a promising model for development of novel class of antibiotics. In this regard it is important to generate Mm overproducing strains which would be a basis for economically justified production of this antibiotic. In this work a set of genes for synthesis and reception of low-molecular weight signaling molecules (LSM) in ATCC14672 were described and their significance for Mm production was studied. The ATCC14672 genome carries structural and regulatory genes for production of LSMs of avenolide and γ-butyrolactone families. Additional copies of LSM biosynthetic genesssfg_07848andssfg_07725did not alter the Mm production level. ATCC14672 LSMs are not capable of restoring the sporulation of butyrolactone-nonproducing mutant ofS. griseus. Likewise, while the heterologous hostS. lividans1326 produced Mm, its mutant M707 (deficient in the butyrolactone synthase genescbA) did not. Thus, while the natural level of LSMs production by ATCC14672 does not limit Mm synthesis, the former is essential for the synthesis of moenomycins.
authors.affiliation: Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Hrushevskoho st. 4, Lviv 79005, Ukraine.
authors.forename: H
authors.initials: H
authors.lastname: Mutenko
authors.affiliation: Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Hrushevskoho st. 4, Lviv 79005, Ukraine.
authors.forename: R
authors.initials: R
authors.lastname: Makitrinskyy
authors.affiliation: Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Hrushevskoho st. 4, Lviv 79005, Ukraine.
authors.forename: O
authors.initials: O
authors.lastname: Tsypik
authors.affiliation: Harvard Medical School, Department of Microbiology and Immunobiology, 4 Blackfan street, Boston, MA 02115, USA.
authors.forename: S
authors.initials: S
authors.lastname: Walker
authors.affiliation: Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Hrushevskoho st. 4, Lviv 79005, Ukraine.
authors.forename: B
authors.initials: B
authors.lastname: Ostash
authors.affiliation: Ivan Franko National University of Lviv, Department of Genetics and Biotechnology, Hrushevskoho st. 4, Lviv 79005, Ukraine.
authors.forename: V
authors.initials: V
authors.lastname: Fedorenko
keywords: A-factor; Streptomyces ghanaensis; avenolide; moenomycin