title: A fast and effective routine method based on HS-SPME-GC-MS/MS for the analysis of organotin compounds in biota samples.
abstract: This work validated an automated, fast, and low solvent- consuming methodology suited for routine analysis of tributyltin (TBT) and degradation products (dibutyltin, DBT; monobutyltin, MBT) in biota samples. The method was based on the headspace solid-phase microextraction methodology (HS-SPME), coupled with gas chromatographic separation and tandem mass-spectrometry (GC-MS/MS). The effectiveness of the matrix-matched signal ratio external calibration was tested for quantification purposes. The exclusion of matrix influences in the calibration curves proved the suitability of this versatile quantification method. The method detection limits obtained were of 3 ng Sn g(-1) dw for all the analytes. The analysis of references materials showed satisfying accuracy under optimum calibration conditions (% recovery between 87-111%; |Z-scores|<2). The repeatability RSD% and intra-laboratory reproducibility RSD% were lower than 9.6% and 12.6%, respectively. The work proved the remarkable analytical performances of the method and its high potential for routine application in monitoring organotin compounds (OTC).
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy. Electronic address: seta.noventa@isprambiente.it.
authors.forename: Seta
authors.initials: S
authors.lastname: Noventa
authors.affiliation: Department of Civil and Environmental Engineering, University of Padua, via Loredan 20, 35131 Padova, Italy.
authors.forename: Jvan
authors.initials: J
authors.lastname: Barbaro
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Formalewicz
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Gion
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Rampazzo
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy.
authors.forename: Rossella Boscolo
authors.initials: RB
authors.lastname: Brusà
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), via Vitaliano Brancati 48, 00144 Roma, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Gabellini
authors.affiliation: National Institute for Environmental Protection and Research (ISPRA), Loc. Brondolo, 30015 Chioggia, Venice, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Berto
mesh_terms: D000818:Animals; D058448:Biota; D049872:Bivalvia / Q000737:chemistry*; D002138:Calibration; D004784:Environmental Monitoring / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D009947:Organotin Compounds / Q000032:analysis*; D012015:Reference Standards; D052617:Solid Phase Microextraction; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D009947:Organotin Compounds; D014874:Water Pollutants, Chemical
keywords: Biota; Calibration; Organotin; Routine; Solid phase microextraction; Tributyltin