title: Molecularly imprinted sol-gel nanofibers based solid phase microextraction coupled on-line with high performance liquid chromatography for selective determination of acesulfame.
abstract: Sol-gel based molecularly imprinted polymer (MIP) nanofiber was successfully fabricated by electrospinning technique on the surface of a stainless steel bar. The manufactured tool was applied for on-line selective solid phase microextraction (SPME) and determination of acesulfame (ACF) as an artificial sweetener with high performance liquid chromatography (HPLC). The selective ability of method for the extraction of ACF was investigated in the presence of some selected sweeteners such as saccharine (SCH), aspartame (ASP) and caffeine (CAF). Electrospinning of MIP sol-gel solution on the stainless steel bar provided an unbreakable sorbent with high thermal, mechanical, and chemical stability. Moreover, application of the MIP-SPME tool revealed a unique approach for the selective microextraction of the analyte in beverage samples. In this work, 3-(triethoxysilyl)-propylamine (TMSPA) was chosen as a precursor due to its ability to imprint the analyte by hydrogen bonding, Van der Walls, and dipole-dipole interactions. Nylon 6 was also added as a backbone and support for the precursor in which sol could greatly growth during the sol-gel process and makes the solution electrospinable. Various effective parameters in the extraction efficiency of the MIP-SPME tool such as loading time, flow rate, desorption time, selectivity, and the sample volume were evaluated. The linearity for the ACF in beverage sample was in the range of 0.78-100.5 ng mL(-1). Limit of detection (LOD) and quantification (LOQ) were 0.23 and 0.78 ng mL(-1) respectively. The RSD values (n=5) were all below 3.5%at the 20 ng mL(-1) level.
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Hafez, Tehran, Iran.
authors.forename: Mohammad Mahdi
authors.initials: MM
authors.lastname: Moein
authors.affiliation: Department of Chemistry, Amirkabir University of Technology, Hafez, Tehran, Iran. Electronic address: mehranjavanbakht@gmail.com.
authors.forename: Mehran
authors.initials: M
authors.lastname: Javanbakht
authors.affiliation: Department of Textile engineering, Amirkabir University of Technology, Hafez, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Karimi
authors.affiliation: Food and Drug Laboratory Research Center, Food and Drug Department, Ministry of Health and Medical Education, Tehran, Iran.
authors.forename: Behrouz
authors.initials: B
authors.lastname: Akbari-Adergani
mesh_terms: D000327:Adsorption; D001628:Beverages / Q000032:analysis*; D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis; D005782:Gels / Q000737:chemistry*; D054802:Molecular Imprinting; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D052617:Solid Phase Microextraction; D013549:Sweetening Agents / Q000032:analysis* / Q000737:chemistry; D013843:Thiazines / Q000032:analysis* / Q000737:chemistry
chemical_list: D005782:Gels; D011108:Polymers; D013549:Sweetening Agents; D013843:Thiazines; C009916:nylon 6; D002209:Caprolactam; C006362:acetosulfame
keywords: Acesulfame; Electrospinning; Molecularly imprinted sol-gel; Solid phase microextraction; Unbreakable