title: [Effects of electro-acupuncture on expression of triggering receptor expressed on myeloid cells 1 in ankle joint synovial tissue of acute gouty arthritis rats].
abstract: OBJECTIVE
  To investigate the effects of electro-acupuncture (EA) on the expression of triggering receptor expressed on myeloid cell (TREM)l in ankle joint synovial tissue of acute gouty arthritis (AGA) rats.
  
  
  METHODS
  Forty male SD rats were randomly divided into 4 groups: normal, AGA, medication and EA group, 10 rats in each group. AGA model was established by induced monosodium urate (MSU) method, except the normal group. Tow days before AGA model was established, normal and AGA groups were lavaged with normal saline (20 ml/kg), medication group was lavaged with colchicine solution (20 ml/kg), EA(1.5-2 Hz, D.-D.wave, 9v; 1-3 rnA) was applied to "Sanyinjiao" (SP6), "jiexi" (ST41) and "Kunlun" (BL60) for 20 min, once daily;continuously for 9 days. Then observed the changes in dysfunction, and the content of TNF-α and IL-lβ detected by ELISA, the expression of TREM-l detected by immunohistochemistry and western blot.
  
  
  RESULTS
  Compared to the normal group, the AGA group of the dysfunction index increased significantly (P<0.01), the content of TNF-α and IL-lβ increased significantly (P<0.05), the expression of TREM-l in synovial tissue increased significantly (P<0.05); the medication and EA groups compared to the AGA group, the dysfunction index decreased significantly (P<0.01), the content of TNF-α and IL-lβ decreased significantly (P<0.05), the expression of TREM-l in synovial tissue decreased significantly (P<0.05); there were not statistically significant between the medication and EA group (P>0.05).
  
  
  CONCLUSIONS
  EA treating AGA may be through down-regulating the expression of TREM -1 in synovial tissue.
authors.affiliation: College of TCM, Chongqing Medical University, Chongqing 401331, China.
authors.forename: Chao-Nan
authors.initials: CN
authors.lastname: Zhang
authors.forename: Xue-Kuan
authors.initials: XK
authors.lastname: Huang
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Juan
authors.initials: J
authors.lastname: Jiang
authors.forename: Lei
authors.initials: L
authors.lastname: Wan
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000378:metabolism / Q000473:pathology*; D015210:Arthritis, Gouty / Q000378:metabolism* / Q000628:therapy; D015671:Electroacupuncture; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic / Q000378:metabolism*; D013583:Synovial Membrane / Q000378:metabolism; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D011971:Receptors, Immunologic; C547511:TREM1 protein, rat; D000073871:Triggering Receptor Expressed on Myeloid Cells-1; D014409:Tumor Necrosis Factor-alpha