title: Sequence and regulation of the porcine FSHR gene promoter.
abstract: Follicle-stimulating hormone (FSH) plays a crucial role in animal reproduction and exerts its physiological functions by interacting with the FSH receptor (FSHR). The FSHR is exclusively expressed in granulose cells in the ovary and its expression level is closely related to granulose cell differentiation and follicle maturation. In mammal, most of the follicles undergo atresia, while follicle atresia is mainly caused by granulosa cell apoptosis. However, knowledge on the transcriptional regulatory mechanisms of the porcine FSHR gene in granulosa cell is still limited. In this study, approximately 2.1kb of the proximal promoter sequence of the porcine FSHR gene were obtained by genome walking, and the regulatory elements and transcription factors in the porcine FSHR promoter sequence were predicted. Furthermore, the core promoter region (-1195/-598) of the porcine FSHR gene was identified using a luciferase assay. Subsequently, the relationship between expression levels of the porcine FSHR gene and histone H3K9 acetylation levels around the core promoter region (-787/-572) in vivo and invitro were analyzed. Our results showed that an increased FSHR gene expression level was accompanied with an increase in histone H3K9 acetylation levels, suggesting that histone H3K9 acetylation could regulate the expression of the porcine FSHR gene.
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: wuwangjun2009@hotmail.com.
authors.forename: Wangjun
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: linzog@yahoo.com.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2011105017@njau.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: Jinbi.zhang@auckland.ac.nz.
authors.forename: Jinbi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012105021@njau.edu.cn.
authors.forename: Bojiang
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liuzequn0806@hotmail.com.
authors.forename: Zequn
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2012105020@njau.edu.cn.
authors.forename: Kaiqing
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liqifa@njau.edu.cn.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: owwa@njau.edu.cn.
authors.forename: Zengxiang
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: jiechen@njau.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: liuhonglin@njau.edu.cn.
authors.forename: Honglin
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D016386:Chromosome Walking; D003001:Cloning, Molecular; D005640:Follicle Stimulating Hormone / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D006657:Histones / Q000378:metabolism; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism*; D013552:Swine / Q000235:genetics / Q000378:metabolism
chemical_list: D006657:Histones; D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone
keywords: FSHR; Granulosa cells; Histone acetylation; Pig; Transcription