title: 2,2'-Bis(monoacylglycero) PO4 (BMP), but Not 3,1'-BMP, increases membrane curvature stress to enhance α-tocopherol transfer protein binding to membranes.
abstract: Previous work revealed that α-tocopherol transfer protein (α-TTP) co-localizes with bis(monoacylglycero)phosphate (BMP) in late endosomes. BMP is a lipid unique to late endosomes and is believed to induce membrane curvature and support the multivesicular nature of this organelle. We examined the effect of BMP on α-TTP binding to membranes using dual polarization interferometry and vesicle-binding assay. α-TTP binding to membranes is increased by the curvature-inducing lipid BMP. α-TTP binds to membranes with greater affinity when they contain the 2,2'-BMP versus 3,1'-BMP isomers.
authors.affiliation: Chemistry and Centre for Biotechnology, Brock University, St. Catharines, Canada.
authors.forename: Matilda
authors.initials: M
authors.lastname: Baptist
authors.forename: Candace
authors.initials: C
authors.lastname: Panagabko
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Nickels
authors.forename: John
authors.initials: J
authors.lastname: Katsaras
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Atkinson
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000502:physiology*; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D007368:Interferometry / Q000379:methods; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D002352:Carrier Proteins; D010710:Phosphates; C017763:alpha-tocopherol transfer protein