title: Bottled water: analysis of mycotoxins by LC-MS/MS.
abstract: The presence of mycotoxins in food samples has been widely studied as well as its impact in human health, however, information about its distribution in the environment is scarce. An analytical method comprising a solid phase extraction procedure followed by liquid chromatography tandem mass spectrometry analysis was implemented and validated for the trace analysis of mycotoxins in drinking bottled waters. Limits of quantification achieved for the method were between 0.2ngL(-1) for aflatoxins and ochratoxin, and 2.0ngL(-1) for fumonisins and neosolaniol. The method was applied to real samples. Aflatoxin B2 was the most frequently detected mycotoxin in water samples, with a maximum concentration of 0.48±0.05ngL(-1) followed by aflatoxin B1, aflatoxin G1 and ochratoxin A. The genera Cladosporium, Fusarium and Penicillium were the fungi more frequently detected. These results show that the consumption of these waters does not represent a toxicological risk for an adult.
authors.affiliation: iMED, Faculdade de Farmácia Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal.
authors.forename: A T
authors.initials: AT
authors.lastname: Mata
authors.affiliation: iMED, Faculdade de Farmácia Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal.
authors.forename: J P
authors.initials: JP
authors.lastname: Ferreira
authors.affiliation: IBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal.
authors.forename: B R
authors.initials: BR
authors.lastname: Oliveira
authors.affiliation: iMED, Faculdade de Farmácia Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal.
authors.forename: M C
authors.initials: MC
authors.lastname: Batoréu
authors.affiliation: IBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, EAN, 2780-157 Oeiras, Portugal.
authors.forename: M T
authors.initials: MT
authors.lastname: Barreto Crespo
authors.affiliation: IBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, EAN, 2780-157 Oeiras, Portugal.
authors.forename: V J
authors.initials: VJ
authors.lastname: Pereira
authors.affiliation: iMED, Faculdade de Farmácia Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-019 Lisboa, Portugal; IBET - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, EAN, 2780-157 Oeiras, Portugal. Electronic address: mrbronze@ff.ul.pt.
authors.forename: M R
authors.initials: MR
authors.lastname: Bronze
mesh_terms: D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods*; D060766:Drinking Water / Q000032:analysis / Q000382:microbiology*; D006801:Humans; D009183:Mycotoxins / Q000032:analysis*; D052616:Solid Phase Extraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D060766:Drinking Water; D009183:Mycotoxins
keywords: Aflatoxin B1 (PubChem CID: 14403); Aflatoxin B2 (PubChem CID: 2724360); Aflatoxin G1 (PubChem CID: 14421); Aflatoxin G2 (PubChem CID: 2724362); Cladosporium; Drinking water; Fumonisin B1 (PubChem CID: 3431); Fumonisin B2 (PubChem CID: 2733489); Fumonisin B3 (PubChem CID: 3034751); Fusarium; LC-ESI-MS/MS; Mycotoxins; Neosolaniol (PubChem CID: 13818797); Ochratoxin A (PubChem CID: 442530); Penicillium