title: Electrical detection of dengue biomarker using egg yolk immunoglobulin as the biological recognition element.
abstract: Nonstructural protein 1 (NS1) is secreted by dengue virus in the first days of infection and acts as an excellent dengue biomarker. Here, the direct electrical detection of NS1 from dengue type 2 virus has been achieved by the measurement of variations in open circuit potential (OCP) between a reference electrode and a disposable Au electrode containing immobilized anti-NS1 antibodies acting as immunosensor. Egg yolk immunoglobulin (IgY) was utilized for the first time as the biological recognition element alternatively to conventional mammalian antibodies in the detection of dengue virus NS1 protein. NS1 protein was detected in standard samples in a 0.1 to 10 µg.mL(-1) concentration range with (3.2 ± 0.3) mV/µg.mL(-1) of sensitivity and 0.09 µg.mL(-1) of detection limit. Therefore, the proposed system can be extended to detect NS1 in real samples and provide an early diagnosis of dengue.
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Figueiredo
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Nirton C S
authors.initials: NC
authors.lastname: Vieira
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Juliana F
authors.initials: JF
authors.lastname: dos Santos
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Bruno C
authors.initials: BC
authors.lastname: Janegitz
authors.affiliation: DNAPTA Biotecnologia, 15035-470, São José do Rio Preto - SP, Brasil.
authors.forename: Sergio M
authors.initials: SM
authors.lastname: Aoki
authors.affiliation: DNAPTA Biotecnologia, 15035-470, São José do Rio Preto - SP, Brasil.
authors.forename: Paulo P
authors.initials: PP
authors.lastname: Junior
authors.affiliation: DNAPTA Biotecnologia, 15035-470, São José do Rio Preto - SP, Brasil.
authors.forename: Rodrigo L
authors.initials: RL
authors.lastname: Lovato
authors.affiliation: Departamento de Doenças Infecciosas e Parasitárias, FAMERP, 15090-000, São José do Rio Preto - SP, Brasil.
authors.forename: Maurício L
authors.initials: ML
authors.lastname: Nogueira
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Valtencir
authors.initials: V
authors.lastname: Zucolotto
authors.affiliation: Nanomedicine and Nanotoxicology Group, Instituto de Física de São Carlos, Universidade de São Paulo, CP 369, 13560-970, São Carlos - SP, Brasil.
authors.forename: Francisco E G
authors.initials: FE
authors.lastname: Guimarães
mesh_terms: D000914:Antibodies, Viral / Q000276:immunology* / Q000302:isolation & purification; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology; D015415:Biomarkers / Q000737:chemistry; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D004530:Egg Yolk / Q000276:immunology*; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay; D007136:Immunoglobulins / Q000302:isolation & purification; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D015415:Biomarkers; D007136:Immunoglobulins; C082515:NS1 protein, dengue-1 virus; D017361:Viral Nonstructural Proteins