title: Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries.
abstract: The volatile fractions of Cape gooseberry and blueberry were determined by headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (HS-SPME/GC×GC-TOFMS). The highest amount of alcohol (51.8%), ester (32.8%) and carboxylic acid (6.9%) was in blueberry in comparison with gooseberry and oppositely ketones (14.7%), aldehydes (9.9%) and terpenes (8%) were found in gooseberry. The bioactive compounds and antioxidant capacities were higher in blueberries than in gooseberries. Three dimensional fluorescence emission spectrometry (3D-FL) was applied to determine and to compare experimentally found binding parameters of berries extracts with human serum albumin (HSA). The fluorescence quenching of HSA by polyphenols from berries was a result of the formation of a polyphenol-HSA complex. The binding abilities of berries were highly correlated with the bioactivity of polyphenols and volatile substances. The cluster analysis (CA) and linear discriminant analysis (LDA) was applied to differentiate the berries samples according to their type.
authors.affiliation: Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, Gdańsk 80 952, Poland. Electronic address: tomasz.dymerski@gmail.com.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Dymerski
authors.affiliation: Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, Gdańsk 80 952, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Namieśnik
authors.affiliation: Faculty of Pharmacy, Srinakharinwirot University, Bangkok, Thailand.
authors.forename: Kann
authors.initials: K
authors.lastname: Vearasilp
authors.affiliation: Department of Basic Sciences, Universidad del Bio-Bio, P.O. Box 447, 3780000 Chillan, Chile.
authors.forename: Patricia
authors.initials: P
authors.lastname: Arancibia-Avila
authors.affiliation: Department of Basic Sciences, Universidad del Bio-Bio, P.O. Box 447, 3780000 Chillan, Chile.
authors.forename: Fernando
authors.initials: F
authors.lastname: Toledo
authors.affiliation: The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
authors.forename: Moshe
authors.initials: M
authors.lastname: Weisz
authors.affiliation: The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
authors.forename: Elena
authors.initials: E
authors.lastname: Katrich
authors.affiliation: The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: shela.gorin@mail.huji.ac.il.
authors.forename: Shela
authors.initials: S
authors.lastname: Gorinstein
mesh_terms: D029798:Blueberry Plants; D005470:Fluorometry; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D059808:Polyphenols / Q000737:chemistry; D031965:Ribes; D012709:Serum Albumin / Q000737:chemistry; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry
chemical_list: D059808:Polyphenols; D012709:Serum Albumin; D055549:Volatile Organic Compounds
keywords: Bioactivity; Three-dimensional fluorescence spectroscopy; Two-dimensional gas chromatography; Volatile substances