title: Melghiribacillus thermohalophilus gen. nov., sp. nov., a novel filamentous, endospore-forming, thermophilic and halophilic bacterium.
abstract: A novel filamentous, endospore-forming, thermophilic and moderately halophilic bacterium designated strain Nari2A(T) was isolated from soil collected from an Algerian salt lake, Chott Melghir. The novel isolate was Gram-staining-positive, aerobic, catalase-negative and oxidase-positive. Optimum growth occurred at 50-55 °C, 7-10% (w/v) NaCl and pH 7-8. The strain exhibited 95.4, 95.4 and 95.2% 16S rRNA gene sequence similarity to Thalassobacillus devorans G19.1(T), Sediminibacillus halophilus EN8d(T) and Virgibacillus kekensis YIM-kkny16(T), respectively. The major menaquinone was MK-7. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three unknown phosphoglycolipids and two unknown phospholipids. The predominant cellular fatty acids were iso-C(15 : 0) and iso-C(17 : 0). The DNA G+C content was 41.9 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain Nari2A(T) is considered to represent a novel species of a new genus in the family Bacillaceae , order Bacillales , for which the name Melghiribacillus thermohalophilus gen. nov., sp. nov. is proposed. The type strain of Melghiribacillus thermohalophilus is Nari2A(T) ( = DSM 25894(T) = CCUG 62543(T)).
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire (Equipe de Microbiologie), Université des Sciences et de la Technologie Houari Boumediène, BP 32, El Alia 16111 Bab Ezzouar, Algiers, Algeria.|Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
authors.forename: Nariman Ammara
authors.initials: NA
authors.lastname: Addou
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
authors.forename: Wajdi
authors.initials: W
authors.lastname: Ben Hania
authors.affiliation: Laboratoire de Biologie Cellulaire et Moléculaire (Equipe de Microbiologie), Université des Sciences et de la Technologie Houari Boumediène, BP 32, El Alia 16111 Bab Ezzouar, Algiers, Algeria.
authors.forename: Hocine
authors.initials: H
authors.lastname: Hacene
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
authors.forename: Guy
authors.initials: G
authors.lastname: Fauque
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, MIO UM 110, 13288 Marseille, France.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000462:Algeria; D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7