title: Self-powered microneedle-based biosensors for pain-free high-accuracy measurement of glycaemia in interstitial fluid.
abstract: In this work a novel self-powered microneedle-based transdermal biosensor for pain-free high-accuracy real-time measurement of glycaemia in interstitial fluid (ISF) is reported. The proposed transdermal biosensor makes use of an array of silicon-dioxide hollow microneedles that are about one order of magnitude both smaller (borehole down to 4µm) and more densely-packed (up to 1×10(6)needles/cm(2)) than state-of-the-art microneedles used for biosensing so far. This allows self-powered (i.e. pump-free) uptake of ISF to be carried out with high efficacy and reliability in a few seconds (uptake rate up to 1µl/s) by exploiting capillarity in the microneedles. By coupling the microneedles operating under capillary-action with an enzymatic glucose biosensor integrated on the back-side of the needle-chip, glucose measurements are performed with high accuracy (±20% of the actual glucose level for 96% of measures) and reproducibility (coefficient of variation 8.56%) in real-time (30s) over the range 0-630mg/dl, thus significantly improving microneedle-based biosensor performance with respect to the state-of-the-art.
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa, via G. Caruso 16, 56122 Pisa, Italy.
authors.forename: L M
authors.initials: LM
authors.lastname: Strambini
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa, via G. Caruso 16, 56122 Pisa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Longo
authors.affiliation: Dipartimento di Chimica 'Ugo Schiff', Università di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Scarano
authors.affiliation: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche (CNR), via G. Moruzzi, 1, 56124 Pisa, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Prescimone
authors.affiliation: Dipartimento di Chimica 'Ugo Schiff', Università di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Palchetti
authors.affiliation: Dipartimento di Chimica 'Ugo Schiff', Università di Firenze, via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Minunni
authors.affiliation: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche (CNR), via G. Moruzzi, 1, 56124 Pisa, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Giannessi
authors.affiliation: Dipartimento di Ingegneria dell'Informazione, Università di Pisa, via G. Caruso 16, 56122 Pisa, Italy; Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche (CNR), via G. Moruzzi, 1, 56124 Pisa, Italy. Electronic address: g.barillaro@iet.unipi.it.
authors.forename: G
authors.initials: G
authors.lastname: Barillaro
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D003920:Diabetes Mellitus / Q000175:diagnosis* / Q000473:pathology; D045604:Extracellular Fluid / Q000737:chemistry; D006801:Humans; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D001786:Blood Glucose; D012822:Silicon Dioxide
keywords: Biosensors; Diabetes; Glycaemia; Microneedles; Pain-free