title: Enhancement on reactive oxygen species and COX-1 mRNA levels modulate the vascular relaxation induced by sodium nitroprusside in denuded mice aorta.
abstract: This study aimed to investigate the modulation of nitric oxide/reactive oxygen species in sodium nitroprusside relaxation in mice aorta. Sodium nitroprusside induced relaxation in endothelium-intact (e+) and endothelium-denuded (e-) aortas with greater potency in e+ than in e-. The nitric oxide synthase inhibitor did not alter the sodium nitroprusside relaxation in both e+ and e- aortas. However, the superoxide anion scavenger abolished the difference in sodium nitroprusside potency between e+ and e-. Sodium nitroprusside reduced dihydroethidium-derived fluorescent products in both groups; however, the difference between intact and denuded mice aorta remains. The glutathione levels and basal antioxidant activity of superoxide dismutase were reduced in e- aorta when compared with e+, and these values were not altered by sodium nitroprusside. Confirming these results, the levels of lipid peroxidation in e+ were significantly lower when compared to e-, and these values were not altered by sodium nitroprusside. The sodium nitroprusside potency in the presence of a nonselective COX inhibitor or the EP/DP prostaglandin receptor antagonist in endothelium denuded was similar to that in intact mice aorta. Based on these results, we performed the COX-1 and COX-2 mRNA level studies, and in denuded mice aorta, there was an upregulation in COX-1 mRNA levels. Taken together, our findings show that in the absence of endothelium, there is an enhancement of superoxide levels, leading to GSH consumption and higher levels of lipid peroxidation, showing an intense redox status. Furthermore, in denuded mice aorta, there was an upregulation of COX-1 mRNA expression, leading to vasoconstrictor prostanoids synthesis. The interaction of vasoconstrictor prostanoids with its receptors EP/DP negatively modulates the vascular relaxation induced by SNP in denuded mice aorta.
authors.affiliation: Departamento de Farmacologia, ICB, Universidade Federal de Minas Gerais, 31.270-901, Belo Horizonte-MG, Brazil.
authors.forename: Lucas M
authors.initials: LM
authors.lastname: Kangussu
authors.forename: Vania C
authors.initials: VC
authors.lastname: Olivon
authors.forename: Raquel D do N
authors.initials: RD
authors.lastname: Arifa
authors.forename: Natália
authors.initials: N
authors.lastname: Araújo
authors.forename: Daniela
authors.initials: D
authors.lastname: Reis
authors.forename: Marieta T de A
authors.initials: MT
authors.lastname: Assis
authors.forename: Frederico M
authors.initials: FM
authors.lastname: Soriani
authors.forename: Daniele da G
authors.initials: Dda G
authors.lastname: de Souza
authors.forename: Lusiane M
authors.initials: LM
authors.lastname: Bendhack
authors.forename: Daniella
authors.initials: D
authors.lastname: Bonaventura
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects / Q000378:metabolism*; D051545:Cyclooxygenase 1 / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D051379:Mice; D008810:Mice, Inbred C57BL; D009599:Nitroprusside / Q000494:pharmacology*; D009924:Organ Culture Techniques; D012333:RNA, Messenger / Q000096:biosynthesis*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D008565:Membrane Proteins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014665:Vasodilator Agents; D009599:Nitroprusside; D051545:Cyclooxygenase 1; C496512:Ptgs1 protein, mouse
keywords: Sodium nitroprusside; cyclo-oxygenase; mice aorta; reactive oxygen species; vascular endothelium