title: Composite scaffolds for controlled drug release: role of the polyurethane nanoparticles on the physical properties and cell behaviour.
abstract: Localised delivery of appropriate biomolecule/drug(s) can be suitable to prevent postoperative infections and inflammation after scaffold implantation in vivo. In this study composite shell scaffolds, based on an internally produced bioactive glass and a commercial hydroxyapatite, were surface coated with a uniform polymeric layer, embedded with thermo-stable polyesterurethane (PU)-based nanoparticles (NPs), containing an anti-inflammatory drug (indomethacin; IDCM). The obtained functionalised scaffolds were subjected to physico-mechanical and biological characterisations. The results indicated that NPs incorporation into the gelatin coating of the composite scaffolds: 1) not changed significantly the micro-architecture of the scaffolds in terms of mean pore diameter and pore size distribution; 2) increased the compressive modulus; and 3) allowed to a sustained IDMC release (65-70% of the loaded-drug) within the first week of incubation in physiological solution. On the other hand, the NPs incorporation did not affect the biocompatibility of composite scaffolds, as evidenced by viability and alkaline phosphatase (ALP) activity of MG63 human osteoblast-like cells.
authors.affiliation: School of Clinical Dentistry, University of Sheffield, 19 Claremont Crescent, Sheffield S102TA, United Kingdom; Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Corso Duca degli Abruzzi 24, Turin 10129, Italy. Electronic address: p.gentile@sheffield.ac.uk.
authors.forename: Piergiorgio
authors.initials: P
authors.lastname: Gentile
authors.affiliation: University of Modena and Reggio Emilia, Department of Engineering "E. Ferrari", Via Pietro Vivarelli 10, Modena 41125, Italy.
authors.forename: Devis
authors.initials: D
authors.lastname: Bellucci
authors.affiliation: University of Modena and Reggio Emilia, Department of Engineering "E. Ferrari", Via Pietro Vivarelli 10, Modena 41125, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Sola
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Corso Duca degli Abruzzi 24, Turin 10129, Italy.
authors.forename: Clara
authors.initials: C
authors.lastname: Mattu
authors.affiliation: University of Modena and Reggio Emilia, Department of Engineering "E. Ferrari", Via Pietro Vivarelli 10, Modena 41125, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Cannillo
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Corso Duca degli Abruzzi 24, Turin 10129, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Ciardelli
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006801:Humans; D007213:Indomethacin / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D055585:Physical Phenomena; D011140:Polyurethanes / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D011140:Polyurethanes; D017886:Durapatite; D000469:Alkaline Phosphatase; D007213:Indomethacin
keywords: Bone tissue engineering; Composite scaffolds; Gelatin coating; Indomethacin; Nanoparticles