title: Isolation and characteristic analysis of a novel strain H7N9 of avian influenza virus A from a patient with influenza-like symptoms in China.
abstract: A novel H7N9 virus (A/Changsha/1/2013(H7N9)) identified through routine examination in the influenza network laboratory was analyzed retrospectively. The gene sequences of A/Changsha/1/2013(H7N9) were highly homologous to other viruses isolated in mainland China. Mutations of Q226L and G186V were found in the hemagglutinin protein (HA). Amino acid deletions were found at positions 69-73 of the neuraminidase protein (NA) and 218-230 of the non-structural protein (NS1). All viral genes except PB1 were essentially identical to the sequences of other Chinese influenza A H7N9 isolates. Overall, A/Changsha/1/2013(H7N9) is highly homologous to other H7N9 avian influenza viruses isolated in mainland China.
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Faming
authors.initials: F
authors.lastname: Chen
authors.affiliation: Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Li
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Biancheng
authors.initials: B
authors.lastname: Sun
authors.affiliation: Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Rusheng
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China. Electronic address: xhouteam@163.com.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Ou
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ye
authors.affiliation: Changsha Center for Disease Control and Prevention, Changsha, Hunan, 410001, China.
authors.forename: Jingfang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, China.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan, China.
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D002675:Child, Preschool; D002681:China; D004252:DNA Mutational Analysis; D005814:Genes, Viral; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D010802:Phylogeny; D017384:Sequence Deletion
keywords: Avian influenza virus; Genetic analysis; H7N9; Influenza surveillance; Mutations