title: In vivo effect of flucloxacillin in experimental endocarditis caused by mecC-positive staphylococcus aureus showing temperature-dependent susceptibility in vitro.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene (mecC-MRSA) exhibited at 37°C MICs of oxacillin close to those of methicillin-susceptible S. aureus (MSSA). We investigated whether at this temperature, mecC-MRSA strains respond to flucloxacillin treatment like MSSA strains, using a rat model of endocarditis. Flucloxacillin (human-like kinetics of 2 g intravenously every 6 h) cured 80 to 100% of aortic vegetations infected with five different mecC-MRSA strains. These results suggest that mecC-MRSA infections may successfully respond to treatment with β-lactams.
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Stefano
authors.initials: S
authors.lastname: Mancini
authors.affiliation: National Reference Centre for Staphylococci, Hospices Civils de Lyon, International Center of Infectious Research, INSERM U1111, University of Lyon, Lyon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laurent
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Tiago R
authors.initials: TR
authors.lastname: Veloso
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Marlyse
authors.initials: M
authors.lastname: Giddey
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Jacques
authors.initials: J
authors.lastname: Vouillamoz
authors.affiliation: National Reference Centre for Staphylococci, Hospices Civils de Lyon, International Center of Infectious Research, INSERM U1111, University of Lyon, Lyon, France.
authors.forename: François
authors.initials: F
authors.lastname: Vandenesch
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Philippe
authors.initials: P
authors.lastname: Moreillon
authors.affiliation: Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland jose.entenza@unil.ch.
authors.forename: José M
authors.initials: JM
authors.lastname: Entenza
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001011:Aorta / Q000382:microbiology; D002440:Cefoxitin / Q000494:pharmacology; D020374:Chromatography, Micellar Electrokinetic Capillary; D004697:Endocarditis, Bacterial / Q000188:drug therapy* / Q000382:microbiology; D005436:Floxacillin / Q000008:administration & dosage / Q000627:therapeutic use*; D007260:Infusion Pumps; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010068:Oxacillin / Q000494:pharmacology; D051381:Rats; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents; D005436:Floxacillin; D002440:Cefoxitin; D010068:Oxacillin