title: Novel insights into nickel import in Staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-type nickel chelator.
abstract: Staphylococcus aureus possesses two canonical ABC-importers dedicated to nickel acquisition: the NikABCDE and the CntABCDF systems, active under different growth conditions. This study reports on the extracytoplasmic nickel-binding components SaNikA and SaCntA. We showed by protein crystallography that SaNikA is able to bind either a Ni-(l-His)2 complex or a Ni-(l-His) (2-methyl-thiazolidine dicarboxylate) complex, depending on their availability in culture supernatants. Native mass spectrometry experiments on SaCntA revealed that it binds the Ni(ii) ion via a different histidine-dependent chelator but it cannot bind Ni-(l-His)2. In vitro experiments are consistent with in vivo nickel content measurements that showed that l-histidine has a high positive impact on nickel import via the Cnt system. These results suggest that although both systems may require free histidine, they use different strategies to import nickel.
authors.affiliation: Univ. Grenoble Alpes, Institut de Biologie Structurale (IBS), F-38044 Grenoble, France.
authors.forename: H
authors.initials: H
authors.lastname: Lebrette
authors.forename: E
authors.initials: E
authors.lastname: Borezée-Durant
authors.forename: L
authors.initials: L
authors.lastname: Martin
authors.forename: P
authors.initials: P
authors.lastname: Richaud
authors.forename: E
authors.initials: E
authors.lastname: Boeri Erba
authors.forename: C
authors.initials: C
authors.lastname: Cavazza
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D003461:Crystallography; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D013058:Mass Spectrometry; D009532:Nickel / Q000737:chemistry*; D011487:Protein Conformation; D013211:Staphylococcus aureus / Q000378:metabolism*; D053778:Thiazolidines / Q000737:chemistry*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002614:Chelating Agents; D053778:Thiazolidines; D006639:Histidine; D009532:Nickel