title: Cytotoxic activity and protein binding through an unusual oxidative mechanism by an iridium(I)-NHC complex.
abstract: A new NHC iridium(I) complex (1) showing significant antiproliferative properties in vitro is described here. Its crystal structure, solution behaviour and interactions with the model proteins cytochrome c (cyt c) and lysozyme were investigated. High resolution ESI-MS measurements suggest that this iridium(i) complex acts as a prodrug and binds cyt c tightly through an unusual "oxidative" mechanism. Eventually, an iridium(III)-NHC fragment is found associated to the protein.
authors.affiliation: Inorganic Chemistry I - Bioinorganic Chemistry, Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstrasse 150, 44801 Bochum, Germany. nils.metzler-nolte@rub.de.
authors.forename: Y
authors.initials: Y
authors.lastname: Gothe
authors.forename: T
authors.initials: T
authors.lastname: Marzo
authors.forename: L
authors.initials: L
authors.lastname: Messori
authors.forename: N
authors.initials: N
authors.lastname: Metzler-Nolte
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D045304:Cytochromes c / Q000737:chemistry; D057809:HEK293 Cells; D019073:HT29 Cells; D006571:Heterocyclic Compounds / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D007495:Iridium / Q000737:chemistry* / Q000494:pharmacology; D061986:MCF-7 Cells; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D009113:Muramidase / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D056831:Coordination Complexes; D006571:Heterocyclic Compounds; C030011:carbene; D007495:Iridium; D045304:Cytochromes c; D009113:Muramidase; D008697:Methane