title: Inteins as traceless purification tags for unnatural amino acid proteins.
abstract: The expression of proteins containing unnatural amino acids through suppression of a stop codon can be limited by truncation due to competition with release factors. When the site of incorporation is near the C-terminus, it may not be feasible to separate the full-length unnatural amino acid protein from the truncated form. We report a simple, traceless procedure that allows one to isolate the desired protein using a C-terminal intein fusion.
authors.affiliation: Department of Chemistry, University of Pennsylvania , 231 South 34th Street, Philadelphia, Pennsylvania 19104, United States.
authors.forename: Solongo
authors.initials: S
authors.lastname: Batjargal
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Walters
authors.forename: E James
authors.initials: EJ
authors.lastname: Petersson
mesh_terms: D000596:Amino Acids; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D047668:Inteins; D008958:Models, Molecular; D011487:Protein Conformation
chemical_list: D000596:Amino Acids; D019843:Archaeal Proteins