title: N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery.
abstract: Polysaccharide-based device for oral delivery of heparin (HP) was successfully prepared. Previously synthesized N,N-dimethyl chitosan (DMC) (86% dimethylated by (1)H NMR spectroscopy) was complexed with HP by mixing HP and DMC aqueous solutions (both at pH 3.0). The polyelectrolyte complex (PEC) obtention was confirmed by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA/DTG) and wide-angle X-ray scattering (WAXS). In vitro controlled release assays of HP from PEC were investigated in the simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). The PEC efficiently protected the HP in SGF condition in which HP is degraded. On the other hand, in SIF PEC promoted the releasing of 80 ± 1.5% of loaded HP. The promissory results indicated that the PEC based on DMC/HP presented potential as drug-carrier matrix, since biological activity of HP was improved at pH close to physiological condition.
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Pedro V A
authors.initials: PV
authors.lastname: Bueno
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Souza
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Heveline D M
authors.initials: HD
authors.lastname: Follmann
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brazil.
authors.forename: Antonio G B
authors.initials: AG
authors.lastname: Pereira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil; Universidade Tecnológica Federal do Paraná (UTFPR), Estrada para Boa Esperança, CEP 85660-000 Dois Vizinhos, Paraná, Brazil. Electronic address: afmartins50@yahoo.com.br.
authors.forename: Alessandro F
authors.initials: AF
authors.lastname: Martins
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Adley F
authors.initials: AF
authors.lastname: Rubira
authors.affiliation: Grupo de Materiais Poliméricos e Compósitos, GMPC, Departamento de Química, Universidade Estadual de Maringá UEM, Av. Colombo 5790, CEP 87020-900 Maringá, Paraná, Brazil.
authors.forename: Edvani C
authors.initials: EC
authors.lastname: Muniz
mesh_terms: D001826:Body Fluids / Q000737:chemistry; D048271:Chitosan / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004573:Electrolytes / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry* / Q000494:pharmacology*; D008962:Models, Theoretical; D066244:Proton Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D004337:Drug Carriers; D004573:Electrolytes; C000603496:N,N-dimethyl chitosan; D006493:Heparin; D048271:Chitosan
keywords: Heparin; N,N-Dimethyl chitosan; Polyelectrolyte complex