title: Magnetic field-guided cell delivery with nanoparticle-loaded human corneal endothelial cells.
abstract: To improve the delivery and integration of cell therapy using magnetic cell guidance for replacement of corneal endothelium, here we assess magnetic nanoparticles' (MNPs') effects on human corneal endothelial cells (HCECs) in vitro. Biocompatible, 50 nm superparamagnetic nanoparticles endocytosed by cultured HCECs induced no short- or long-term change in viability or identity. Assessment of guidance of the magnetic HCECs in the presence of different magnet shapes and field strengths showed a 2.4-fold increase in delivered cell density compared to gravity alone. After cell delivery, HCECs formed a functional monolayer, with no difference in tight junction formation between MNP-loaded and control HCECs. These data suggest that nanoparticle-mediated magnetic cell delivery may increase the efficiency of cell delivery without compromising HCEC survival, identity or function. Future studies may assess the safety and efficacy of this therapeutic modality in vivo. From the clinical editor: The authors show in this article that magnetic force facilitates the delivery of human corneal endothelial cells loaded by superparamagnetic nanoparticles to cornea, without changing their morphology, identity or functional properties. This novel idea can potentially have vast impact in the treatment of corneal endothelial dystrophies by providing self-endothelial cells after ex-vivo expansion.
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Stavros N
authors.initials: SN
authors.lastname: Moysidis
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Alvarez-Delfin
authors.affiliation: MD/PhD Program in Neuroscience University of Miami Miller School of Medicine, Miami, FL, USA; Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Veronica J
authors.initials: VJ
authors.lastname: Peschansky
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Enrique
authors.initials: E
authors.lastname: Salero
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Alejandra D
authors.initials: AD
authors.lastname: Weisman
authors.affiliation: Shiley Eye Center, University of California San Diego, La Jolla, CA, USA.
authors.forename: Alena
authors.initials: A
authors.lastname: Bartakova
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Gabriella A
authors.initials: GA
authors.lastname: Raffa
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Merkhofer
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA; Shiley Eye Center, University of California San Diego, La Jolla, CA, USA.
authors.forename: Karl E
authors.initials: KE
authors.lastname: Kador
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA; Shiley Eye Center, University of California San Diego, La Jolla, CA, USA; Emmetrope Ophthalmics LLC, Key Biscayne, FL, USA.
authors.forename: Noelia J
authors.initials: NJ
authors.lastname: Kunzevitzky
authors.affiliation: Bascom Palmer Eye Institute and Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA; Shiley Eye Center, University of California San Diego, La Jolla, CA, USA. Electronic address: jlgoldberg@ucsd.edu.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Goldberg
mesh_terms: D002478:Cells, Cultured; D003315:Cornea / Q000166:cytology / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism* / Q000637:transplantation; D006801:Humans; D060526:Magnetic Fields; D053758:Nanoparticles / Q000737:chemistry*
keywords: Cell therapy; Corneal edema; Human corneal endothelial cells; Magnetic nanoparticles