title: Comparing antioxidant capacity of purine alkaloids: a new, efficient trio for screening and discovering potential antioxidants in vitro and in vivo.
abstract: The most commonly applied strategies for the evaluation of antioxidant capacity are the chemical- or cell-based approaches. However, the results obtained from these methods might not reflect the antioxidant ability of test samples within organisms. In this study, we propose a combination of experiments, including oxygen radical absorbance capacity (ORAC), cellular antioxidant activity assay (CAA), and the chick embryo model, as an efficient trio to evaluate antioxidant capacity of food components. Taking purine alkaloids as example, results demonstrate that chemical and cellular method might misinterpret their true ability on antioxidation. In chick embryo model, caffeine and theacrine can significantly improve vessel density on chorioallantoic membrane and myocardial apoptosis. The mechanism can be involving multiple targets within the organism. We believe that the trio proposed can be widely utilized in screening massive number of antioxidant in a cost-effective way. It will also help discovering new antioxidants that are easily being omitted due to their relatively poor in vitro activities.
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Bun
authors.initials: B
authors.lastname: Tsoi
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Ruo-Nan
authors.initials: RN
authors.lastname: Yi
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Ling-Fang
authors.initials: LF
authors.lastname: Cao
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Shan-Bing
authors.initials: SB
authors.lastname: Li
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Rui-Rong
authors.initials: RR
authors.lastname: Tan
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Xiao-Xiao
authors.initials: XX
authors.lastname: Li
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China. Electronic address: liyifangkele@sina.com.
authors.forename: Yi-Fang
authors.initials: YF
authors.lastname: Li
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kurihara
authors.affiliation: Anti-Stress and Health Research Center, Pharmacy College, Jinan University, Guangzhou 510632, China. Electronic address: rongronghe66@163.com.
authors.forename: Rong-Rong
authors.initials: RR
authors.lastname: He
mesh_terms: D000470:Alkaloids; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002110:Caffeine; D002642:Chick Embryo; D066298:In Vitro Techniques / Q000379:methods*; D010084:Oxidation-Reduction; D011687:Purines / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D000975:Antioxidants; D011687:Purines; D002110:Caffeine; C030985:purine
keywords: Antioxidant capacity; CAA; Caffeine; Chick embryo; ORAC; Purine alkaloids; Theacrine