title: [Proliferation inhibition and apoptosis induction of Juglone on human cervical cancer Caski cells].
abstract: OBJECTIVE
  To explore the effects of Juglone on proliferation and apoptosis of human cervical cancer Caski cells, and to further study the related mechanism of cell apoptosis.
  
  
  METHODS
  Cultured Caski cells were incubated with 20, 40, 60, 80 and 100 μmol/L juglone for 24 h. The proliferation of Caski cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The cell apoptosis were detected by transmission electron microscope. The expression of Bcl-2 and Bax were detected by Western blot.
  
  
  RESULTS
  MTT results showed that in different doses of juglone groups, the Caski cell growth was greatly inhibited (P < 0.05, P < 0.01) and showed dose dependent when compared with control group except 20 μmol/L. The IC50 of juglone was 42.4 μmol/L. After treatment on Caski cells with 40 μmol/L juglone, typical apoptosis characteristics was observed by transmission electronmicro scope. The expression of Bcl-2 was decreased while the expression of Bax was increased significantly when compared with control group (P < 0.05).
  
  
  CONCLUSIONS
  Juglone significantly inhibits the proliferation and induces the apoptosis of Caski cells in vitro.
authors.affiliation: Jilin Medical College, Jilin 132013, China. jlmmczw@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Luo
authors.forename: Xingyu
authors.initials: X
authors.lastname: Zhao
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.forename: Wenhe
authors.initials: W
authors.lastname: Zhu
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Jiang
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002453:Cell Cycle / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009285:Naphthoquinones / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; D009285:Naphthoquinones; D051028:bcl-2-Associated X Protein; C005134:juglone