title: MiR-155 targets TP53INP1 to regulate liver cancer stem cell acquisition and self-renewal.
abstract: In liver cancer, miR-155 up-regulation can regulate cancer-cell invasion. However, whether miR-155 expression is associated with liver cancer stem cells (CSCs) remains unknown. Here, we show that miR-155 expression is up-regulated in tumor spheres. Knock-down of miR-155 resulted in suppression of tumor sphere formation, through a decrease in the proportion of CD90(+) and CD133(+) CSCs and in the expression of Oct4, whereas miR-155 overexpression had the opposite effect. TP53INP1 was determined to be involved in the CSCs-like properties that were regulated by miR-155. Thus, miR-155 may play an important role in promoting the generation of stem cell-like cells and their self-renewal by targeting the gene TP53INP1.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Fengchao
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Chongqing Medical University, Chongqing, China. Electronic address: g6j6@sohu.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms; D035683:MicroRNAs / Q000502:physiology*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D034622:RNA Interference; D018800:Thy-1 Antigens / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D002352:Carrier Proteins; D006023:Glycoproteins; D006360:Heat-Shock Proteins; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; C000606023:PROM1 protein, human; D010455:Peptides; C429767:TP53INP1 protein, human; D018800:Thy-1 Antigens
keywords: Liver cancer; Liver cancer stem cell; MiR-155; Tumor Protein 53-Induced Nuclear Protein 1; Tumor sphere