title: Distribution of serotonin and dopamine in the central nervous system of the female mud crab, Scylla olivacea (Herbst).
abstract: In crustaceans serotonin (5-HT) and dopamine (DA) are neurotransmitters that play roles in the modulation of numerous physiological functions, including reproduction. However, in the mud crab, Scylla olivacea, the distributions of 5-HT and DA in the CNS have not yet been investigated. The aim of our study was to map the distributions of these two neurotransmitters in the central nervous system (CNS) of the female of this crab during the late stage of ovarian development. We found 5-HT immunoreactivity (-ir) and DA-ir in many parts of the CNS, including the eyestalk, brain, and thoracic ganglia. In the eyestalk, 5-HT-ir was localized in the medulla terminalis (MT), hemi-ellipsoid body (HB), and protocerebral tract (PT), whereas DA-ir was present in neuronal cluster 1, the LG neuropils, and PT. In the brain, 5-HT-ir and DA-ir were detected in cells and fibers of neuronal clusters 6, 7, 8, 9, 10, 11, 14, and 15. In the ventral nerve cord, 5-HT-ir was present in neurons of the abdominal ganglia, whereas DA was only present in fibers. These spatial distributions of 5-HT and DA suggest that they may be involved in the neuromodulation of important physiological functions, including ovarian maturation, as shown in other non-crab decapods.
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand. Electronic address: kanjana5325@gmail.com.
authors.forename: Kanjana
authors.initials: K
authors.lastname: Khornchatri
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12121, Thailand.
authors.forename: Napamanee
authors.initials: N
authors.lastname: Kornthong
authors.affiliation: Department of Anatomy, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Jirawat
authors.initials: J
authors.lastname: Saetan
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand.
authors.forename: Yotsawan
authors.initials: Y
authors.lastname: Tinikul
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Mahidol University, Nakhonsawan Campus, Nakhonsawan 60130, Thailand.
authors.forename: Charoonroj
authors.initials: C
authors.lastname: Chotwiwatthanakun
authors.affiliation: Faculty of Science, Health and Education, University of the Sunshine Coast, QLD 4558, Australia.
authors.forename: Scott F
authors.initials: SF
authors.lastname: Cummins
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand; Pro Vice-Chancellor's Office, Faculty of Science, Engineering and Built Environment, Deakin University, Locked Bay 20000, Geelong, VIC 3220, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Hanna
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand. Electronic address: prasert.sob@mahidol.ac.th.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
mesh_terms: D000818:Animals; D003386:Brachyura / Q000378:metabolism*; D002490:Central Nervous System / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D018377:Neurotransmitter Agents / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents; D012701:Serotonin; D004298:Dopamine
keywords: CNS; Dopamine (DA); Immunocytochemistry; Neuronal clusters; Scylla olivacea; Serotonin (5-HT)