title: Genotypic variations in the dynamics of metal concentrations in poplar leaves: a field study with a perspective on phytoremediation.
abstract: Poplar is commonly used for phytoremediation of metal polluted soils. However, the high concentrations of trace elements present in leaves may return to soil upon leaf abscission. To investigate the mechanisms controlling leaf metal content, metal concentrations and expression levels of genes involved in metal transport were monitored at different developmental stages on leaves from different poplar genotypes growing on a contaminated field. Large differences in leaf metal concentrations were observed among genotypes. Whereas Mg was remobilized during senescence, Zn and Cd accumulation continued until leaf abscission in all genotypes. A positive correlation between Natural Resistance Associated Macrophage Protein 1 (NRAMP1) expression levels and Zn bio-concentration factors was observed. Principal component analyses of metal concentrations and gene expression levels clearly discriminated poplar genotypes. This study highlights a general absence of trace element remobilization from poplar leaves despite genotype specificities in the control of leaf metal homeostasis.
authors.affiliation: CNRS, Institut des Sciences du Végétal, UPR 2355, Saclay Plant Sciences, Avenue de la Terrasse, Bât 23A, F-91198 Gif Sur Yvette, France. Electronic address: mathieu.pottier@icloud.com.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pottier
authors.affiliation: Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas, Serrano 115-bis, 28006 Madrid, Spain.
authors.forename: Vanesa S
authors.initials: VS
authors.lastname: García de la Torre
authors.affiliation: CNRS, Institut des Sciences du Végétal, UPR 2355, Saclay Plant Sciences, Avenue de la Terrasse, Bât 23A, F-91198 Gif Sur Yvette, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Victor
authors.affiliation: CNRS, Institut des Sciences du Végétal, UPR 2355, Saclay Plant Sciences, Avenue de la Terrasse, Bât 23A, F-91198 Gif Sur Yvette, France.
authors.forename: Laure C
authors.initials: LC
authors.lastname: David
authors.affiliation: Université de Franche-Comté, Laboratoire Chrono-Environnement, 4 place Tharradin, BP 71427, 25 211 Montbeliard, France; Université de Lorraine, Faculté des Sciences & Technologies, 54506 Vandoeuvre-les-Nancy cedex, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Chalot
authors.affiliation: CNRS, Institut des Sciences du Végétal, UPR 2355, Saclay Plant Sciences, Avenue de la Terrasse, Bât 23A, F-91198 Gif Sur Yvette, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Thomine
mesh_terms: D001673:Biodegradation, Environmental; D002104:Cadmium / Q000378:metabolism; D004787:Environmental Pollution / Q000032:analysis; D008670:Metals / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D032107:Populus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012987:Soil; D012989:Soil Pollutants / Q000032:analysis*; D014131:Trace Elements / Q000032:analysis; D015032:Zinc / Q000032:analysis
chemical_list: D008670:Metals; D012987:Soil; D012989:Soil Pollutants; D014131:Trace Elements; D002104:Cadmium; D015032:Zinc
keywords: Cadmium; Copper; HMA; NRAMP; Zinc