title: Complete chloroplast genome of the orchid Cattleya crispata (Orchidaceae:Laeliinae), a Neotropical rupiculous species.
abstract: A partial genome dataset was sequenced for the orchid Cattleya crispata using both Illumina and 454 technologies. The chloroplast genome was assembled using iterative runs of MIRA software that yielded a circular molecule with 148,343 bp in length and deposited in GenBank database (Accession Number KP168671). The plastid genome conserved the quadripartite structure present in most Orchidaceae chloroplasts and was composed by 79 protein-coding genes, 39 tRNAs and 8 rRNAs. Genome structure, gene order and orientation were similar to previously described chloroplasts for Cymbidium orchids, differing in gene order for petN and psbM genes. Data described here contain the first report of a complete chloroplast for the Neotropical subtribe Laeliinae and may contribute to improve the phylogenetic resolution and allow the development of new molecular markers for population genetic studies of orchids.
authors.affiliation: a Laboratório de Genômica e Biodiversidade , Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.|b Myleus Biotechnology , Belo Horizonte , Brazil.
authors.forename: Violeta
authors.initials: V
authors.lastname: da Rocha Perini
authors.affiliation: c Departamento de Ecologia , Universidade Estadual Paulista , Rio Claro , São Paulo , and.
authors.forename: Bruno
authors.initials: B
authors.lastname: Leles
authors.affiliation: d Division of Genetics , Instituto Nacional do Câncer , Rio de Janeiro , Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Furtado
authors.affiliation: a Laboratório de Genômica e Biodiversidade , Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Francisco
authors.initials: F
authors.lastname: Prosdocimi
mesh_terms: D002736:Chloroplasts / Q000235:genetics*; D030541:Databases, Genetic; D023061:Gene Order / Q000235:genetics; D054628:Genome, Chloroplast / Q000235:genetics*; D029595:Orchidaceae / Q000235:genetics*; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics
chemical_list: D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: Espinhaço; next-generation-sequencing; orchid; plastid genome; rock outcrop