title: Physiological and biochemical characteristics of laboratory induced mutants of Botrytis cinerea with resistance to fluazinam.
abstract: Botrytis cinerea is a necrotrophic and filamentous fungus with a high risk of developing resistance to fungicides. The pyridinamine fungicide fluazinam has been reported to have excellent activity against B. cinerea and better effect on controlling gray mold. In this study, the physiological and biochemical characteristics of laboratory-induced mutants of B. cinerea with resistance to fluazinam has been investigated. Compared to the wild-type strains, the fluazinam-resistant mutants had a significant decrease in respiratory rate, glycerol, oxalate, and ATP contents, and an increase in ATPase activity and sensitivity to osmotic pressure, but did not differ in cell membrane permeability. Sequencing indicated that two parental strains and four resistant mutants were identical in the nucleotide sequence of F-ATPase gene. These results will enrich our understanding of the resistance mechanism of B. cinerea to fluazinam.
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wenyong
authors.initials: W
authors.lastname: Shao
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Weichao
authors.initials: W
authors.lastname: Ren
authors.affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: changjun-chen@njau.edu.cn.
authors.forename: Changjun
authors.initials: C
authors.lastname: Chen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics; D000255:Adenosine Triphosphate / Q000378:metabolism; D000631:Aminopyridines; D001483:Base Sequence; D020171:Botrytis / Q000235:genetics / Q000378:metabolism; D002463:Cell Membrane Permeability; D004271:DNA, Fungal / Q000235:genetics; D025141:Drug Resistance, Fungal / Q000235:genetics / Q000502:physiology; D005656:Fungal Proteins / Q000235:genetics; D005659:Fungicides, Industrial; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D009154:Mutation; D019815:Oxalic Acid / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D000631:Aminopyridines; D004271:DNA, Fungal; D005656:Fungal Proteins; D005659:Fungicides, Industrial; C075780:fluazinam; D000255:Adenosine Triphosphate; D019815:Oxalic Acid; D000251:Adenosine Triphosphatases; D005947:Glucose; D005990:Glycerol
keywords: Botrytis cinerea; Characteristics; Fluazinam; Resistance