title: Wheat bran biorefinery--an insight into the process chain for the production of lactic acid.
abstract: The present study investigates a wheat bran biorefinery including major processing steps pre-extraction, pre-treatment and lactic acid fermentation. Concerning the dry mass distribution, during the pre-extraction 32% of the feedstock was recovered, offering some perspectives for applications in food area. The pre-treatment (hydrothermal/enzymatic hydrolysis) of the remaining cake solubilized 34% of dry mass and led to a fermentable sugar concentration of 21g/L. The fermentation resulted in a lactic acid yield of 0.73g/g substrate. Concentrating the fermentation feed via nanofiltration did not improve the lactic acid productivity. Taking into account that Lactobacillus pentosus, a heterofermentative microorganism was used, the dry mass balance revealed a product yield of 47% (32% extract, 15% lactic acid). Based on a theoretical consideration involving a cellulolytic enzyme production (10% feedstock allocation) and lignin utilization, under optimized conditions a maximum product yield of around 80% (35% extract, 39% lactic acid, 6% lignin) could be expected.
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Muthgasse 18, 1190 Vienna, Austria. Electronic address: oezge.tirpanalan@boku.ac.at.
authors.forename: Özge
authors.initials: Ö
authors.lastname: Tirpanalan
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Reisinger
authors.affiliation: Institute for Environmental Biotechnology, Department for Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Konrad Lorenz Str. 20, 3430 Tulln, Austria.
authors.forename: Marina
authors.initials: M
authors.lastname: Smerilli
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Huber
authors.affiliation: Institute for Environmental Biotechnology, Department for Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Konrad Lorenz Str. 20, 3430 Tulln, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Neureiter
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kneifel
authors.affiliation: Department of Food Science and Technology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria; Christian Doppler Research Laboratory for Innovative Bran Biorefinery, Muthgasse 18, 1190 Vienna, Austria.
authors.forename: Senad
authors.initials: S
authors.lastname: Novalin
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004043:Dietary Fiber; D005285:Fermentation; D005374:Filtration / Q000379:methods; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism; D008031:Lignin / Q000378:metabolism
chemical_list: D004043:Dietary Fiber; C036909:lignocellulose; D019344:Lactic Acid; D008031:Lignin
keywords: Biorefinery; Fermentation; Lactic acid; Lignocellulose; Wheat bran