title: Positional cloning in maize (Zea mays subsp. mays, Poaceae).
abstract: OBJECTIVE
  Positional (or map-based) cloning is a common approach to identify the molecular lesions causing mutant phenotypes. Despite its large and complex genome, positional cloning has been recently shown to be feasible in maize, opening up a diverse collection of mutants to molecular characterization. •
  
  
  RESULTS
  Here we outline a general protocol for positional cloning in maize. While the general strategy is similar to that used in other plant species, we focus on the unique resources and approaches that should be considered when applied to maize mutants. •
  
  
  CONCLUSIONS
  Positional cloning approaches are appropriate for maize mutants and quantitative traits, opening up to molecular characterization the large array of genetic diversity in this agronomically important species. The cloning approach described should be broadly applicable to other species as more plant genomes become available.
authors.affiliation: Waksman Institute of Microbiology, Rutgers University, Piscataway, New Jersey 08854-8020 USA ; Department of Plant Biology and Pathology, Rutgers University, New Brunswick, New Jersey 08901 USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Gallavotti
authors.affiliation: Department of Biology, Brigham Young University, Provo, Utah 84602 USA.
authors.forename: Clinton J
authors.initials: CJ
authors.lastname: Whipple
keywords: bulked segregant analysis; maize; mapping; positional cloning; simple sequence repeat (SSR) marker