title: LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo.
abstract: LukS-PV, a component of Panton-Valentine leukocidin (PVL) secreted by Staphylococcus aureus, has been shown to inhibit proliferation and induce apoptosis in acute myeloid leukemia (AML) THP-1 cells. Here we investigated anti-leukemia activities of LukS-PV in HL-60 cells, using in vitro assays to assess the ability of LukS-PV to mediate cell viability, apoptosis and differentiation; and developing a Severe Combined Immunodeficiency (SCID) mouse model of disseminated AML with the HL-60 cells to examine in vivo anti-leukemia activity. LukS-PV inhibited viability and induced differentiation and apoptosis in the HL-60 AML cell line. In the SCID mice, LukS-PV potently inhibited tumor growth, decreased tumor cell infiltration into peripheral blood and tissues, and significantly increased mean survival time. No severe adverse effects, such as death, weight loss, or pathological changes in livers or spleens were observed in the toxicity test group. These results indicate that LukS-PV may be a novel and effective chemotherapeutic agent against AML.
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Wulin
authors.initials: W
authors.lastname: Shan
authors.affiliation: Clinical Laboratory Center, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Bu
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Chengfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: School of Medicine, Shandong University, Jinan, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Bangsheng
authors.initials: B
authors.lastname: Ding
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Chang
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Anhui Provincial Laboratories of Pathogen Biology and Zoonoses, Department of Microbiology and Parasitology, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Jilong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Laboratory Medicine, Anhui Provincial Hospital, Anhui Medical University, Hefei 230032, PR China. Electronic address: xiaolingma@126.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001427:Bacterial Toxins / Q000378:metabolism* / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D005098:Exotoxins / Q000378:metabolism* / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D066298:In Vitro Techniques; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007956:Leukocidins / Q000378:metabolism* / Q000494:pharmacology*; D008297:Male; D051379:Mice; D016513:Mice, SCID; D021122:Protein Subunits; D011897:Random Allocation; D023041:Xenograft Model Antitumor Assays
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D021122:Protein Subunits
keywords: Acute myeloid leukemia; Apoptosis; Bacteriotoxin; Differentiation; Panton-Valentine leukocidin