title: VCP/Cdc48 rescues the growth defect of a GPI10 mutant in yeast.
abstract: We identified a yeast mutant with temperature-sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhibited increased unfolded protein response, which was partially rescued by VCP or Cdc48, and showed sensitivity against cell-wall stressors, which were not rescued by VCP. These results suggest a potential link between VCP/Cdc48 and Gpi10 functions in the control of cell growth.
authors.affiliation: Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies, Kyoto 606-8501, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ohnuma
authors.affiliation: Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies, Kyoto 606-8501, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Takata
authors.affiliation: Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Kawawaki
authors.affiliation: Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies, Kyoto 606-8501, Japan; Department of Anatomy and Neurobiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8523, Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yasuda
authors.affiliation: Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo 156-8506, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Laboratory of Protein Metabolism, Tokyo Metropolitan Institute of Medical Science, Setagaya-ku, Tokyo 156-8506, Japan; Department of Applied Biological Chemistry, Graduate School of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address: kimura.yoko@shizuoka.ac.jp.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Kimura
authors.affiliation: Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies, Kyoto 606-8501, Japan. Electronic address: kakizuka@lif.kyoto-u.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Kakizuka
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008364:Mannosyltransferases / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D013696:Temperature; D000074405:Valosin Containing Protein
chemical_list: D018797:Cell Cycle Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C484448:GPI10 protein, S cerevisiae; D008364:Mannosyltransferases; D000251:Adenosine Triphosphatases; C000614177:CDC48 protein, S cerevisiae; D000074405:Valosin Containing Protein
keywords: Cdc48; Endoplasmic reticulum; GPI-anchor; Gpi10; Temperature-sensitivity; VCP