title: Polymerase chain reaction-based serotyping of pathogenic bacteria in food.
abstract: Serotyping analysis of bacterial pathogens in food products is important for foodborne disease surveillance and outbreak investigations. Traditional immunological techniques are labor-intensive and time-consuming, whereas polymerase chain r eaction (PCR)-based techniques are more robust, consistent and rapid. PCR-based methods also provide easier standardization and better reproducibility. Here, we summarize some recent developments and applications of PCR-based serotyping for common foodborne pathogens, and provide a list of available bioinformatics tools for developing PCR-based serotyping assays.
authors.affiliation: Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park, IL, USA. Electronic address: Joelle.Salazar@fda.hhs.gov.
authors.forename: Joelle K
authors.initials: JK
authors.lastname: Salazar
authors.affiliation: Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park, IL, USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Resource and Environmental Engineering, Jiangxi Key Laboratory of Mining and Metallurgy Environmental Pollution Control, Jiangxi University of Science and Technology, Ganzhou, Jiangxi, China.
authors.forename: Shuijing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Key Laboratory of Food Safety Research, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Food Science & Nutrition, Illinois Institute of Technology, Bedford Park, IL, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
mesh_terms: D004269:DNA, Bacterial; D019453:Escherichia coli O157 / Q000145:classification* / Q000235:genetics; D005516:Food Microbiology; D008089:Listeria monocytogenes / Q000145:classification* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*; D012984:Software
chemical_list: D004269:DNA, Bacterial
keywords: E. coli; Food contamination; Listeria; PCR; Salmonella; Serotyping