title: Genome-wide gene expression profiling of ischemia-reperfusion injury in rat kidney, intestine and skeletal muscle implicate a common involvement of MAPK signaling pathway.
abstract: The mechanisms of ischemia‑reperfusion (I/R) injury have not been fully elucidated to date. In order to determine the genetic involvement across different organs during I/R injury, a DNA microarray approach was used to analyze the gene expression profiles of the kidney, intestine, and skeletal muscle in a rat model of I/R injury. Fifteen male Lewis rats were divided randomly into three different organ groups; a sham operation (control group), 60‑min‑ischemia (Is group) only, and 60‑min‑ischemia plus 60‑min‑reperfusion (I/R group), respectively. The target genes were identified by DNA microarray and studied by quantitative polymerase chain reaction (qPCR). By comparing the I/R group with the control group, a 2‑fold upregulation of 467, 172, and 3932 and a 2‑fold downregulation of 437, 416, and 4203 genes were identified in the kidney, small intestine, and skeletal muscle, respectively. Several commonly upregulated genes associated with mitogen‑activated protein kinase (MAPK) pathways, including Jun, Atf3, junB, Fos, Adm and Dusp 1, were differentially expressed in the I/R group. The mRNA expression levels of the target genes were confirmed by qPCR. The present study hypothesized that the MAPK pathway may function in a common pathway of I/R injury and regulate the pathogenesis through activator protein 1. The findings of the present study contributed to the understanding of the molecular pathways associated with I/R injury.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou Medical Center, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Nai-Jen
authors.initials: NJ
authors.lastname: Chang
authors.affiliation: Department of Chemical Engineering and Biotechnology, and Graduate Institute of Biotechnology, National Taipei University of Technology, Taipei, Taiwan, R.O.C.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Weng
authors.affiliation: College of Medicine, Chang Gung University, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Kuo-Hsuan
authors.initials: KH
authors.lastname: Chang
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou Medical Center, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Eric Kar-Wai
authors.initials: EK
authors.lastname: Liu
authors.affiliation: College of Medicine, Chang Gung University, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Cheng-Keng
authors.initials: CK
authors.lastname: Chuang
authors.affiliation: Department of Pediatric Surgery, Taipei Medical University Hospital, Taipei, Taiwan, R.O.C.
authors.forename: Chih-Cheng
authors.initials: CC
authors.lastname: Luo
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou Medical Center, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Cheng-Hung
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Linkou Medical Center, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: Fu-Chan
authors.initials: FC
authors.lastname: Wei
authors.affiliation: College of Medicine, Chang Gung University, Tao‑Yuan, Taiwan, R.O.C.
authors.forename: See-Tong
authors.initials: ST
authors.lastname: Pang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D020935:MAP Kinase Signaling System; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D009928:Organ Specificity / Q000235:genetics; D051381:Rats; D015427:Reperfusion Injury / Q000235:genetics* / Q000378:metabolism*; D015203:Reproducibility of Results; D059467:Transcriptome