title: Temporal-spatial variations of the physicochemical characteristics of air pollution Particulate Matter (PM2.5-0.3) and toxicological effects in human bronchial epithelial cells (BEAS-2B).
abstract: While the evidence for the health adverse effects of air pollution Particulate Matter (PM) has been growing, there is still uncertainty as to which constituents within PM are most harmful. Hence, to contribute to fulfill this gap of knowledge, some physicochemical characteristics and toxicological endpoints (i.e. cytotoxicity, oxidative damage, cytokine secretion) of PM2.5-0.3 samples produced during two different seasons (i.e. spring/summer or autumn/winter) in three different surroundings (i.e. rural, urban, or industrial) were studied, thereby expecting to differentiate their respective adverse effects in human bronchial epithelial cells (BEAS-2B). Physicochemical characteristics were closely related to respective origins and seasons of the six PM2.5-0.3 samples, highlighting the respective contributions of industrial and heavy motor vehicle traffic sources. Space- and season-dependent differences in cytotoxicity of the six PM2.5-0.3 samples could only be supported by considering both the physicochemical properties and the variance in air PM concentrations. Whatever spaces and seasons, dose- and even time-dependent increases in oxidative damage and cytokine secretion were reported in PM2.5-0.3-exposed BEAS-2B cells. However, the relationship between the chemical composition of each of the six PM2.5-0.3 samples and their oxidative or inflammatory potentials seemed to be very complex. These results supported the role of inorganic, ionic and organic components as exogenous source of Reactive Oxygen Species and, thereafter, cytokine secretion. Nevertheless, one of the most striking observation was that some inorganic, ionic and organic chemical components were preferentially associated with early oxidative events whereas others in the later oxidative damage and/or cytokine secretion. Taken together, these results indicated that PM mass concentration alone might not be able to explain the health outcomes, because PM is chemically nonspecific, and supported growing evidence that PM-size, composition and emission source, together with sampling season, interact in a complex manner to produce PM2.5-0.3-induced human adverse health effects.
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Mona
authors.initials: M
authors.lastname: Dergham
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Capucine
authors.initials: C
authors.lastname: Lepers
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Verdin
authors.affiliation: Université Lille Nord de France, Lille, France; Centre Commun de Mesures, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Cazier
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Billet
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Courcot
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France.
authors.forename: Pirouz
authors.initials: P
authors.lastname: Shirali
authors.affiliation: Université Lille Nord de France, Lille, France; EA 4492, Université du Littoral-Côte d'Opale, Dunkerque, France; EA4483, Université de Lille 2, Lille, France. Electronic address: guillaume.garcon@univ-lille2.fr.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Garçon
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D002460:Cell Line; D004784:Environmental Monitoring; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D015999:Multivariate Analysis; D010316:Particle Size; D052638:Particulate Matter / Q000633:toxicity*; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects*
chemical_list: D000393:Air Pollutants; D052638:Particulate Matter; D017382:Reactive Oxygen Species
keywords: Air pollution PM; BEAS-2B cells; Chemical determinant; Cytokine secretion; Lung toxicity; Oxidative stress; Physicochemical characterization; Temporal–spatial variations