title: Amino acids substitutions in the PB2 protein of H7N9 influenza A viruses are important for virulence in mammalian hosts.
abstract: We tested the biological significance of two amino acid mutations in the PB2 protein (glutamic acid to lysine at position 627 and aspartic acid to asparagine at position 701) of A(H7N9) viruses for mammalian adaptation. Mutants were assessed for their viral polymerase activities, growth kinetics in mammalian and avian cells, and pathogenicity in mice. We found that lysine at position 627 and asparagine at position 701 in PB2 are essential for mammalian adaptation of A(H7N9) viruses.
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Yamayoshi
authors.affiliation: 1] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuyama
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yamada
authors.affiliation: 1] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.
authors.forename: Dongming
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Murakami
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Uraki
authors.affiliation: 1] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.
authors.forename: Tokiko
authors.initials: T
authors.lastname: Watanabe
authors.affiliation: 1] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Tomita
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI, USA.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Neumann
authors.affiliation: 1] Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan [2] ERATO Infection-Induced Host Responses Project, Japan Science and Technology Agency, Saitama, Japan [3] Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan [4] Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 575 Science Drive, Madison, WI, USA.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kawaoka
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001835:Body Weight; D002460:Cell Line; D004285:Dogs; D005260:Female; D017930:Genes, Reporter; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000378:metabolism* / Q000472:pathogenicity; D008168:Lung / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D009296:Nasal Cavity / Q000821:virology; D014764:Viral Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics*; D014779:Virus Replication
chemical_list: C502893:PB2 protein, influenza virus; D014764:Viral Proteins