title: Role of seaweed laminaran from Saccharina longicruris on matrix deposition during dermal tissue-engineered production.
abstract: Our laboratory has developed a technique to reconstruct in vitro tissue from human cells using the self-assembly tissue-engineering method, which utilizes the ability of fibroblasts to deposit the matrix they secrete. The time necessary for tissue construction, several weeks, is a drawback for many clinical uses. We hypothesized that the addition of laminaran can increase the deposition of matrix, speeding up the production of the tissue. Laminaran was isolated from the brown seaweed Saccharina longicruris harvested in Canada and its structure was evaluated. Laminaran is a small molecular weight polysaccharide composed of linear glucose chains. Monolayer-cultured human skin fibroblasts were cultured in the presence of laminaran with ascorbate for 7 or 35 days to produce a dermis. Treatment did not induce any variation in the growth rate or alpha smooth muscle actin content but it did increase the deposition of collagen I in a dose-dependent manner. After 35 days, the reconstructed dermal thickness was increased when laminaran was added, and collagen I deposition and MMP activity were also significantly increased. Thus, laminaran can be used to increase the rate of production of reconstructed self-assembled dermis and can also potentially be used in cosmetic or therapeutic creams to stimulate matrix production.
authors.affiliation: Centre de recherche en organogenese experimentale de l'Universite Laval/LOEX, Division of Regenerative Medicine, CHU de Quebec research center/FRQS, Faculty of Medicine, Universite Laval, Quebec city, Canada.
authors.forename: Akram
authors.initials: A
authors.lastname: Ayoub
authors.affiliation: Centre de recherche en organogenese experimentale de l'Universite Laval/LOEX, Division of Regenerative Medicine, CHU de Quebec research center/FRQS, Faculty of Medicine, Universite Laval, Quebec city, Canada.
authors.forename: Jadson Moreira
authors.initials: JM
authors.lastname: Pereira
authors.affiliation: Institute on Nutrition and Functional Foods, Department of Food Science, Universite Laval, Quebec city, Canada.
authors.forename: Laurie-Eve
authors.initials: LE
authors.lastname: Rioux
authors.affiliation: Institute on Nutrition and Functional Foods, Department of Food Science, Universite Laval, Quebec city, Canada.
authors.forename: Sylvie L
authors.initials: SL
authors.lastname: Turgeon
authors.affiliation: Rimouski, Quebec city, Canada.
authors.forename: Martin
authors.initials: M
authors.lastname: Beaulieu
authors.affiliation: Centre de recherche en organogenese experimentale de l'Universite Laval/LOEX, Division of Regenerative Medicine, CHU de Quebec research center/FRQS, Faculty of Medicine, Universite Laval, Quebec city, Canada. Electronic address: Veronique.moulin@fmed.ulaval.ca.
authors.forename: Véronique J
authors.initials: VJ
authors.lastname: Moulin
mesh_terms: D000199:Actins / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D002452:Cell Count; D024042:Collagen Type I / Q000378:metabolism; D020405:Dermis / Q000187:drug effects / Q000502:physiology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005936:Glucans / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D012624:Seaweed / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D055815:Young Adult
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D005936:Glucans; D053773:Transforming Growth Factor beta1; C008247:laminaran; D020782:Matrix Metalloproteinases
keywords: Fibroblast; Laminaran; Tissue-engineering method