title: Plasticity of mesenchymal stem cells from mouse bone marrow in the presence of conditioned medium of the facial nerve and fibroblast growth factor-2.
abstract: A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.
authors.affiliation: Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
authors.forename: Eudes Euler de Souza
authors.initials: EE
authors.lastname: Lucena
authors.affiliation: Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
authors.forename: Fausto Pierdoná
authors.initials: FP
authors.lastname: Guzen
authors.affiliation: Laboratory of Experimental Neurology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
authors.forename: José Rodolfo Lopes de Paiva
authors.initials: JR
authors.lastname: Cavalcanti
authors.affiliation: Laboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
authors.forename: Maria Jocileide de Medeiros
authors.initials: MJ
authors.lastname: Marinho
authors.affiliation: Laboratory of Molecular Biology, Health Science Center, State University of Rio Grande do Norte, Avenida Atirador Miguel da Silva Neto, Airport, 59607-360 Mossoró-RN, Brazil.
authors.forename: Wogelsanger Oliveira
authors.initials: WO
authors.lastname: Pereira
authors.affiliation: Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil.
authors.forename: Carlos Augusto Galvão
authors.initials: CA
authors.lastname: Barboza
authors.affiliation: Laboratory of Neuroanatomy, Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil.
authors.forename: Miriam Stela Mariz de Oliveira
authors.initials: MS
authors.lastname: Costa
authors.affiliation: Laboratory of Neuroanatomy, Department of Morphology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil.
authors.forename: Expedito Silva
authors.initials: ES
authors.lastname: do Nascimento Júnior
authors.affiliation: Laboratory of Neurochemical Studies, Department of Physiology, Bioscience Center, Federal University of Rio Grande do Norte, University Campus, Lagoa Nova, 59078-900 Natal, RN, Brazil.
authors.forename: Jeferson Sousa
authors.initials: JS
authors.lastname: Cavalcante
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D005154:Facial Nerve / Q000254:growth & development / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D051381:Rats
chemical_list: D017077:Culture Media, Conditioned; D016222:Fibroblast Growth Factor 2