title: Peanut protein sensitivity towards trace iron: a novel mode to ebb allergic response.
abstract: Peanut is a rich source of plant protein which is inexpensive and abundant in nature. The peanut proteins however cause hypersensitive immunogenic responses in certain individuals. A minute amount of contamination may cause strong allergic reactions and even death. Many chemical pretreatment procedures have been developed and prescribed earlier for removal of this allergenicity. In the present article we have observed trace level Fe(III) and Cu(II) complexation of the protein fractions of peanut at pH 4.8 using different spectral methods. Consequently we studied the allergic response of Fe(III) complex of the protein fractions using competitive enzyme linked immunosorbent assay (ELISA) technique and found that there were considerable losses in allergenicity of conarachin I and arachin fractions upon complexation. Immunoassay of Cu(II) complex was avoided keeping in view the Cu toxicity in living systems. The results bring up a new strategy towards reduction of allergenicity using an inexpensive and simple methodology.
authors.affiliation: Department of Chemistry, University of Calcutta, 92, APC Road, Kolkata 700 009, India.
authors.forename: Sumanta Kumar
authors.initials: SK
authors.lastname: Ghatak
authors.affiliation: Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India.
authors.forename: Dipanwita
authors.initials: D
authors.lastname: Majumdar
authors.affiliation: Department of Physics, Bose Institute, 93/1, Acharya Prafulla Chandra Road, Kolkata 700009, India.
authors.forename: Achintya
authors.initials: A
authors.lastname: Singha
authors.affiliation: Mugneeram Ramkowar Bangur Hospital, 241-Desapran Sasmal Road, Kolkata 700 033, India.
authors.forename: Souvik
authors.initials: S
authors.lastname: Sen
authors.affiliation: Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064, India.
authors.forename: Debashree
authors.initials: D
authors.lastname: Das
authors.affiliation: Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700 064, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
authors.affiliation: Department of Chemistry, University of Calcutta, 92, APC Road, Kolkata 700 009, India.
authors.forename: Chaitali
authors.initials: C
authors.lastname: Mukhopadhyay
authors.affiliation: Department of Chemistry, University of Calcutta, 92, APC Road, Kolkata 700 009, India. Electronic address: kamalchem.roy@gmail.com.
authors.forename: Kamalika
authors.initials: K
authors.lastname: Sen
mesh_terms: D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D010367:Arachis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005296:Ferrous Compounds / Q000276:immunology* / Q000378:metabolism; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D010940:Plant Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000485:Allergens; D005296:Ferrous Compounds; D010940:Plant Proteins; C000102:Arachin
keywords: ELISA; Immunoassay; Peanut proteins; Spectroscopy; Trace elements