title: Nanobiocomposite of poly(lactide-co-glycolide)/chitosan electrospun scaffold can promote proliferation and transdifferentiation of Schwann-like cells from human adipose-derived stem cells.
abstract: The transdifferentiation of human adipose-derived stem cells (ADSCs) into Schwann-like cells on biocomposite scaffolds may be a critical issue in nerve regeneration medicine. In this study, tissue-engineered scaffold with chitosan (CS) nanopowders and poly(lactide-co-glycolide) (PLGA) was investigated for its potential Schwann cells (SCs) transdifferentiation. The differentiation of human ADSCs into S-like cells was induced with different CS content and direction of nanofibers on PLGA/CS scaffolds. Cell morphology and proliferation of differentiated cells were investigated by scanning electron microscopy and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay respectively. For assessment efficiency of transdifferentiation, the expression of SC markers (glial fibrillary acidic protein and S100), and myelinogenic marker (myelin basic protein) was investigated in different nanochitosan content and direction of nanofibers scaffolds, using immunocytochemistry technique. The nanochitosan can significantly promote cell proliferation of differentiated cells (p < 0.05). The mean percentage of S-like cells on greater CS content nanofibers scaffold was significantly higher than others (p < 0.05). In addition, the align orientation of nanofibers in scaffolds guided the differentiation of ADSCs toward myelinating S-like cells on the constructs. Overall, we found that high CS content and aligned-orientation of nanofibers in biocomposite scaffold (70/30A) can promote differentiation and myelinogenic capacity of S-like cells induced from human ADSCs.
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran.
authors.forename: Shahnaz
authors.initials: S
authors.lastname: Razavi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Isfahan, 81746-73441, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Zarkesh-Esfahani
authors.affiliation: Department of Textile Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Morshed
authors.affiliation: Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran.|Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Vaezifar
authors.affiliation: Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 15875-4413, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Karbasi
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Golozar
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D001672:Biocompatible Materials; D002454:Cell Differentiation; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053761:Nanocomposites; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012583:Schwann Cells / Q000166:cytology*; D013234:Stem Cells / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan
keywords: ADSCs; PLGA/CS; Schwann-like cell; nanocomposite; transdifferentiation