title: Amaryllidaceae alkaloids from the bulbs of Lycoris radiata with cytotoxic and anti-inflammatory activities.
abstract: Four new Amaryllidaceae alkaloids, (+)-1-hydroxy-ungeremine (1), (+)-6β-acetyl-8-hydroxy-9-methoxy-crinamine (2), (+)-2-hydroxy-8-demethyl-homolycorine-α-N-oxide (3), (+)-N-methoxylcarbonyl-2-demethyl-isocorydione (4), together with two known compounds, (+)-6β-acetyl-crinamine (5) and 8-demethyl-homolycorine-α-N-oxide (6) were isolated from the ethanol extract of the bulbs of Lycoris radiata. Structural elucidation of all the compounds were performed by spectral methods such as 1D and 2D ((1)H-(1)H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. All the isolated alkaloids were in vitro evaluated for their cytotoxic activities against eight tumor cell lines (BEN-MEN-1, CCF-STTG1, CHG-5, SHG-44, U251, BGC-823, HepG2 and SK-OV-3) and anti-inflammatory activities against Cox-1 and Cox-2. As a result, alkaloids 1 and 4 exhibited significant cytotoxic activities against all tested tumor cell lines except against BEN-MEN-1. Additionally, alkaloids 1 and 4 possessed selective inhibition of Cox-2 comparable with the standard drug NS-398 (>90%).
authors.affiliation: Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Road, Changsha, Hunan 410011, China; The First People Hospital of Qujing, Qujing 655000, Yunnan Province, China. Electronic address: liuzhiming.cn@163.com.
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Liu
authors.affiliation: Qujing Medical College, Qujing 655000, Yunnan Province, China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Huang
authors.affiliation: The First People Hospital of Qujing, Qujing 655000, Yunnan Province, China.
authors.forename: Mao-Rong
authors.initials: MR
authors.lastname: Cui
authors.affiliation: The First People Hospital of Qujing, Qujing 655000, Yunnan Province, China.
authors.forename: Xiao-De
authors.initials: XD
authors.lastname: Zhang
authors.affiliation: The First People Hospital of Qujing, Qujing 655000, Yunnan Province, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Qujing Medical College, Qujing 655000, Yunnan Province, China.
authors.forename: Ben-Shou
authors.initials: BS
authors.lastname: Yang
authors.affiliation: Second Xiangya Hospital, Central South University, No. 139 Middle Renmin Road, Changsha, Hunan 410011, China.
authors.forename: Xiao-Kun
authors.initials: XK
authors.lastname: Zhao
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry*; D045744:Cell Line, Tumor; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry*; D006801:Humans; D007212:Indolizines / Q000737:chemistry*; D031426:Lycoris / Q000737:chemistry*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry
chemical_list: D047151:Amaryllidaceae Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D016861:Cyclooxygenase Inhibitors; D007212:Indolizines; C016175:ungeremine; C411129:crinamine
keywords: Alkaloids; Amaryllidaceae; Anti-inflammatory activity; Cytotoxicity; Lycoris radiata