title: Application of mixed cloud point extraction for the analysis of six flavonoids in Apocynum venetum leaf samples by high performance liquid chromatography.
abstract: A simple, inexpensive and efficient method based on the mixed cloud point extraction (MCPE) combined with high performance liquid chromatography was developed for the simultaneous separation and determination of six flavonoids (rutin, hyperoside, quercetin-3-O-sophoroside, isoquercitrin, astragalin and quercetin) in Apocynum venetum leaf samples. The non-ionic surfactant Genapol X-080 and cetyl-trimethyl ammonium bromide (CTAB) was chosen as the mixed extracting solvent. Parameters that affect the MCPE processes, such as the content of Genapol X-080 and CTAB, pH, salt content, extraction temperature and time were investigated and optimized. Under the optimized conditions, the calibration curve for six flavonoids were all linear with the correlation coefficients greater than 0.9994. The intra-day and inter-day precision (RSD) were below 8.1% and the limits of detection (LOD) for the six flavonoids were 1.2-5.0 ng mL(-1) (S/N=3). The proposed method was successfully used to separate and determine the six flavonoids in A. venetum leaf samples.
authors.affiliation: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department Clinical Laboratory, No. 23 Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Jiang Bing
authors.initials: JB
authors.lastname: Sun
authors.affiliation: Department of Traditional Chinese Medicine, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Xin Yu
authors.initials: XY
authors.lastname: Xu
authors.affiliation: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Zhao Hui
authors.initials: ZH
authors.lastname: Cheng
authors.affiliation: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.affiliation: Department of Chemistry, Fourth Military Medical University, Xian, Shanxi 710032, China.
authors.forename: Feng Qiao
authors.initials: FQ
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China. Electronic address: sjbzj415@163.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D030010:Apocynum / Q000737:chemistry*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005419:Flavonoids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D044949:Kaempferols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry; D012431:Rutin / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D002593:Cetrimonium Compounds; D005419:Flavonoids; D044949:Kaempferols; D010936:Plant Extracts; D013501:Surface-Active Agents; C016527:isoquercitrin; C055545:quercetin-3-O-sophoroside; D011092:Polyethylene Glycols; D012431:Rutin; C021304:hyperoside; C116996:genapol X 080; D011794:Quercetin; C001579:astragalin; D000077286:Cetrimonium
keywords: Apocynum venetum; CTAB; Flavonoids; Genapol X-080; Mixed cloud point extraction