title: Biosynthetic origin of E-resveratrol accumulation in grape canes during postharvest storage.
abstract: Grape canes are vineyard waste products containing valuable phytochemicals of medicine and agriculture interest. Grape canes storage is critical for the accumulation of these bioactive compounds. In the present study, we investigated the changes in stilbenoid phytochemical composition during grape cane storage and the influence of the temperature on final concentrations. A strong increase in the concentration of the monomer E-resveratrol (approximately 40-fold) was observed during the first 6 weeks of storage at 20 °C in eight different grape varieties without any change in oligomer concentrations. The E-resveratrol accumulation was temperature-dependent with an optimal range at 15-20 °C. A 2 h heat-shock treatment aiming at protein denaturation inhibited E-resveratrol accumulation. The constitutive expression of key genes involved in the stilbene precursor biosynthesis along with an induction of stilbene synthase (STS) expression during the first weeks of storage contribute to a de novo biosynthesis of E-resveratrol in pruned wood grapes.
authors.affiliation: Biomolécules et Biotechnologies Végétales, EA 2106, Université François-Rabelais de Tours , F-37200 Tours, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Houillé
authors.forename: Sébastien
authors.initials: S
authors.lastname: Besseau
authors.forename: Vincent
authors.initials: V
authors.lastname: Courdavault
authors.forename: Audrey
authors.initials: A
authors.lastname: Oudin
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Glévarec
authors.forename: Guillaume
authors.initials: G
authors.lastname: Delanoue
authors.forename: Laurence
authors.initials: L
authors.lastname: Guérin
authors.forename: Andrew John
authors.initials: AJ
authors.lastname: Simkin
authors.forename: Nicolas
authors.initials: N
authors.lastname: Papon
authors.forename: Marc
authors.initials: M
authors.lastname: Clastre
authors.forename: Nathalie
authors.initials: N
authors.lastname: Giglioli-Guivarc'h
authors.forename: Arnaud
authors.initials: A
authors.lastname: Lanoue
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis / Q000096:biosynthesis*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry* / Q000378:metabolism; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis / Q000378:metabolism*; D013696:Temperature; D027843:Vitis / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D014866:Waste Products / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D013267:Stilbenes; D014866:Waste Products; D000217:Acyltransferases; C022674:stilbene synthase; D000077185:Resveratrol
keywords: grape cane; postharvest storage; stilbene synthase; stilbenoids