title: Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system.
abstract: PorM is a membrane protein involved in the assembly of the type IX secretion system (T9SS) from Porphyromonas gingivalis, a major bacterial pathogen responsible for periodontal disease in humans. The periplasmic domain of PorM was overexpressed in Escherichia coli and purified. A fragment of the purified protein was obtained by limited proteolysis. Crystals of this fragment belonged to the tetragonal space group P4(3)2(1)2. Native and MAD data sets were recorded to 2.85 and 3.1 Å resolution, respectively, using synchrotron radiation.
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Stathopulos
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Cambillau
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), UMR 7255 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Cascales
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Roussel
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS et Aix-Marseille Université, Marseille, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leone
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D058947:Bacterial Secretion Systems; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D016966:Porphyromonas gingivalis; D017434:Protein Structure, Tertiary; D021122:Protein Subunits
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D021122:Protein Subunits
keywords: Porphyromonas gingivalis; T9SS