title: Detection of Mycobacterium tuberculosis by EasyNAT diagnostic kit in sputum samples from Tanzania.
abstract: The EasyNAT assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (TB) patients in an African high-TB and high-HIV setting. The sensitivity of the EasyNAT assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patients. The sensitivity was only 10% in the smear-negative and culture-positive patients.
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania fmhimbira@ihi.or.tz.
authors.forename: Francis A
authors.initials: FA
authors.lastname: Mhimbira
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland.
authors.forename: Maira
authors.initials: M
authors.lastname: Bholla
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sasamalo
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: William
authors.initials: W
authors.lastname: Mukurasi
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania.
authors.forename: Jerry J
authors.initials: JJ
authors.lastname: Hella
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland.
authors.forename: Levan
authors.initials: L
authors.lastname: Jugheli
authors.affiliation: Ifakara Health Institute, Bagamoyo, Tanzania Swiss Tropical and Public Health Institute, Basel, Switzerland University of Basel, Basel, Switzerland.
authors.forename: Klaus
authors.initials: K
authors.lastname: Reither
mesh_terms: D001431:Bacteriological Techniques; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013636:Tanzania / Q000453:epidemiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000453:epidemiology / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic