title: Probing conformational stability and dynamics of erythroid and nonerythroid spectrin: effects of urea and guanidine hydrochloride.
abstract: We have studied the conformational stability of the two homologous membrane skeletal proteins, the erythroid and non-erythroid spectrins, in their dimeric and tetrameric forms respectively during unfolding in the presence of urea and guanidine hydrochloride (GuHCl). Fluorescence and circular dichroism (CD) spectroscopy have been used to study the changes of intrinsic tryptophan fluorescence, anisotropy, far UV-CD and extrinsic fluorescence of bound 1-anilinonapthalene-8-sulfonic acid (ANS). Chemical unfolding of both proteins were reversible and could be described as a two state transition. The folded erythroid spectrin and non-erythroid spectrin were directly converted to unfolded monomer without formation of any intermediate. Fluorescence quenching, anisotropy, ANS binding and dynamic light scattering data suggest that in presence of low concentrations of the denaturants (up-to 1M) hydrogen bonding network and van der Waals interaction play a role inducing changes in quaternary as well as tertiary structures without complete dissociation of the subunits. This is the first report of two large worm like, multi-domain proteins obeying twofold rule which is commonly found in small globular proteins. The free energy of stabilization (ΔGuH20) for the dimeric spectrin has been 20 kcal/mol lesser than the tetrameric from.
authors.affiliation: Chemistry Department, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Malay
authors.initials: M
authors.lastname: Patra
authors.affiliation: Chemistry Department, University of Calcutta, Kolkata, West Bengal, India.
authors.forename: Chaitali
authors.initials: C
authors.lastname: Mukhopadhyay
authors.affiliation: Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, Kolkata, West Bengal, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Chakrabarti
mesh_terms: D000818:Animals; D041905:Erythroid Cells / Q000378:metabolism*; D019791:Guanidine / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D055503:Protein Multimerization; D055550:Protein Stability / Q000187:drug effects; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D013049:Spectrin / Q000737:chemistry* / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology*
chemical_list: D013049:Spectrin; D014508:Urea; D019791:Guanidine