title: Extracts of endophytic fungus xkc-s03 fromPrunella vulgarisL. spica inhibit gastric cancerin vitroandin vivo.
abstract: Prunella vulgarisL. belongs to thePrunellagenus and has been proven effective in the treatment of gastric cancer, however, the therapeutic activity of the endophytic fungi is not yet well understood. The results of the present study suggest that the ethyl acetate extract (S03-EA) of the endophytic fungus XKC-S03, isolated fromPrunella vulgarisL. spica, is a potent anticancer agent with the potential to treat gastric cancer. In the present study, the effects of S03-EA on gastric cancerin vitroandin vivowere determined using the 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay and the human gastric cancer SGC 7901 cell xenograft model. The tumor tissue was fixed with 10% formaldehyde solution and the levels of the apoptotic proteins, B-cell lymphoma protein-2 (Bcl-2), Bcl-2-associated X protein (Bax) and pro-angiogenic vascular endothelial growth factor (VEGF), were measured by immunohistochemistry. The results indicated that treating SGC 7901 cells with petroleum ether (S03-PE), ethyl acetate (S03-EA) or dichloromethane (S03-DM) extracts from the XKC-S03 fermentation broth inhibited cell proliferation. S03-EA demonstrated the best activity, with a half maximal inhibitory concentration of 25.89 μg/ml and dose-dependent suppression of the SGC 7901 tumor cellsin vivo,without any evident adverse effects. In addition, the 100-mg/kg/day S03-EA-treated tumor tissue revealed a downregulation of Bcl-2 and VEGF expression and an upregulation of Bax expression. In conclusion, the S03-EA extract of XKC-S03, isolated fromPrunella vulgarisL. spica, exhibits a growth-suppressive activity on gastric cancerin vitroandin vivo.
authors.affiliation: Department of Surgery, Second Hospital of Zhongnan University, Changsha, Hunan 410011, P.R. China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Tan
authors.affiliation: Department of Surgery, Second Hospital of Zhongnan University, Changsha, Hunan 410011, P.R. China.
authors.forename: Haizhi
authors.initials: H
authors.lastname: Qi
authors.affiliation: Department of Surgery, Second Hospital of Zhongnan University, Changsha, Hunan 410011, P.R. China.
authors.forename: Jiangdon
authors.initials: J
authors.lastname: Ni
keywords: Prunella vulgaris L.; endophytic fungi; ethyl acetate extract; gastric cancer