title: Complexed soluble IL-7 receptor α and IL-7 increase IL-7-mediated proliferation and viability of CD8⁺ T-cells in vitro.
abstract: Many soluble cytokine receptors inhibit cytokine bioactivity, while others prolong ligand activity. The biological role of an endogenous soluble form of IL-7Rα, or its therapeutic effects on CD8(+) T-cells are unknown. We demonstrate that recombinant IL-7Rα-Fc, when pre-incubated with IL-7, enhances IL-7-induced CD8(+) T-cell proliferation and viability of human or murine CD8(+) T-cells. Receptor blocking experiments confirmed IL-7-specific activity. These data demonstrate that exogenous soluble IL-7Rα significantly enhances CD8(+) T-cell responses to IL-7 in vitro and paves the way for future research to determine its therapeutic potential to restore impaired CD8(+) T-cell function in disease.
authors.affiliation: Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada.
authors.forename: Sandra
authors.initials: S
authors.lastname: Côté
authors.affiliation: Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Matte
authors.affiliation: University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada.
authors.forename: Subash
authors.initials: S
authors.lastname: Sad
authors.affiliation: Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada; Ottawa Hospital, Division of Infectious Diseases, Ottawa, ON, Canada.
authors.forename: Jonathan B
authors.initials: JB
authors.lastname: Angel
authors.affiliation: Ottawa Hospital Research Institute, Chronic Disease Program, Ottawa, ON, Canada; University of Ottawa, Department of Biochemistry, Microbiology and Immunology, Ottawa, ON, Canada. Electronic address: acrawley@ohri.ca.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Crawley
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D015851:Interleukin-7 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D020395:Receptors, Interleukin-7 / Q000276:immunology*
chemical_list: D015851:Interleukin-7; D020395:Receptors, Interleukin-7; C415350:interleukin-7 receptor, alpha chain
keywords: CD8(+) T-cells; Cell proliferation; Human; IL-7 receptor; Murine