title: Electroacupuncture of neiguan (PC 6) inhibits enhanced voltage-gated sodium currents in ischemic ventricular myocytes.
abstract: OBJECTIVE
  To examine the effect of electroacupuncture (EA) at bilateral Neiguan (PC 6) on voltage-gated Na+ currents (I(Na)) and channels (Na(v)) in ischemic ventricular myocytes.
  
  
  METHODS
  EA serum was prepared from six male adult Sprague-Dawley rats that had received EA at bilateral Neiguan (PC 6). Eighteen ventricular myocytes were prepared from six SD rats using an enzymolysis approach. Myocardial ischemia was mimicked by perfusion of ischemic solution. Whole-cell patch-clamping was used to record three currents evoked from isolated cells. The first current was the control, and recorded in absence of ischemic solution current. The second was the ischemic current, and recorded after perfusion of ischemic solution for 5 min, while the EA current was last, and recorded after perfusion of EA serum for 5 min. Na(v) kinetic curves were fitted using related formulas.
  
  
  RESULTS
  Compared with those in controls, in the presence of ischemic solution, peak amplitudes of I(Na) significantly increased from - 40 mV to +30 mV, and half-maximal inactivation potentials of Na(v) increased significantly, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Na(v) were unchanged. Compared with those in the ischemic solution, in the presence of EA serum, peak ischemic current amplitudes significantly reduced from - 40 mV to +40 mV, and half-maximal inactivation potentials were restored, while half-maximal activation potentials, slope factors and the recovery time from inactivation to activation of Na(v) were unchanged.
  
  
  CONCLUSIONS
  EA at bilateral Neiguan (PC 6) can reduce enhanced I(Na) via restoration of delayed Na(v) inactivation in ischemic ventricular myocytes.
authors.forename: Baoqiang
authors.initials: B
authors.lastname: Dong
authors.forename: Chunri
authors.initials: C
authors.lastname: Li
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Zhang
authors.forename: Shudong
authors.initials: S
authors.lastname: Wang
authors.forename: Zhedong
authors.initials: Z
authors.lastname: Cheng
authors.forename: Peijing
authors.initials: P
authors.lastname: Rong
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D015671:Electroacupuncture; D022062:Electrophysiologic Techniques, Cardiac; D006352:Heart Ventricles / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism / Q000503:physiopathology* / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D061566:Voltage-Gated Sodium Channels / Q000378:metabolism
chemical_list: D061566:Voltage-Gated Sodium Channels; D012964:Sodium