title: Selection for high and low oxygen consumption altered hepatic mitochondrial energy efficiency in mice.
abstract: Selection for high (H) and low (L) oxygen consumption (OC) as an indirect estimation of maintenance energy requirement was determined. Feed intake and body weight were measured and feed conversion ratio (FCR) of 4-8-week-old mice was calculated. Respiratory activity of hepatic mitochondria was measured at 12 weeks. Total feed intake (H: 103.74 g, L: 97.92 g, P < 0.01), daily feed intake (H: 3.70 g/day, L: 3.50 g/day, P < 0.01) and FCR (H: 18.79, L: 15.50, P < 0.01) were significantly different between lines. The line by sex interaction was significant for FCR. No line differences were observed in males; and the FCR of the H line was greater than in the L line in females. H line mice had the highest hepatic mitochondrial respiratory activity in state 2 (P < 0.01), the highest uncoupled respiratory rate of mitochondria in the presence of an uncoupling agent (P < 0.001), and the mitochondrial proton leak. The adenosine diphosphate/ O ratio was highest in the L line (P < 0.05). This suggests that the selection for high and low OC induced differences in basal mitochondrial respiration and basal metabolism, resulting in difference in FCR between H and L lines.
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Astrid
authors.initials: A
authors.lastname: Ardiyanti
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Motoi
authors.initials: M
authors.lastname: Kikusato
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shimazu
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Toyomizu
authors.affiliation: Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D001835:Body Weight; D004435:Eating / Q000502:physiology*; D004734:Energy Metabolism / Q000502:physiology*; D005260:Female; D008297:Male; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D011522:Protons
chemical_list: D011522:Protons; D000244:Adenosine Diphosphate
keywords: mice; mitochondria; oxygen consumption; selection