title: Complement activation and expression during chronic relapsing experimental autoimmune encephalomyelitis in the Biozzi ABH mouse.
abstract: Chronic relapsing experimental autoimmune encephalomyelitis (crEAE) in mice recapitulates many of the clinical and histopathological features of human multiple sclerosis (MS), making it a preferred model for the disease. In both, adaptive immunity and anti-myelin T cells responses are thought to be important, while in MS a role for innate immunity and complement has emerged. Here we sought to test whether complement is activated in crEAE and important for disease. Disease was induced in Biozzi ABH mice that were terminated at different stages of the disease to assess complement activation and local complement expression in the central nervous system. Complement activation products were abundant in all spinal cord areas examined in acute disease during relapse and in the progressive phase, but were absent in early disease remission, despite significant residual clinical disease. Local expression of C1q and C3 was increased at all stages of disease, while C9 expression was increased only in acute disease; expression of the complement regulators CD55, complement receptor 1-related gene/protein y (Crry) and CD59a was reduced at all stages of the disease compared to naive controls. These data show that complement is activated in the central nervous system in the model and suggest that it is a suitable candidate for exploring whether anti-complement agents might be of benefit in MS.
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: V
authors.initials: V
authors.lastname: Ramaglia
authors.affiliation: Neuroinflammation, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Jackson
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: T R
authors.initials: TR
authors.lastname: Hughes
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: J W
authors.initials: JW
authors.lastname: Neal
authors.affiliation: Neuroinflammation, Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
authors.forename: D
authors.initials: D
authors.lastname: Baker
authors.affiliation: Institute of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
authors.forename: B P
authors.initials: BP
authors.lastname: Morgan
mesh_terms: D000818:Animals; D003167:Complement Activation; D003176:Complement C3 / Q000235:genetics / Q000276:immunology; D003165:Complement System Proteins / Q000235:genetics / Q000276:immunology*; D004195:Disease Models, Animal; D018450:Disease Progression; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D038602:Mice, Biozzi; D020529:Multiple Sclerosis, Relapsing-Remitting
chemical_list: D003176:Complement C3; D003165:Complement System Proteins
keywords: chronic relapsing; complement; experimental autoimmune encephalomyelitis (EAE); multiple sclerosis