title: Pyrosequencing analysis of a bacterial community associated with lava-formed soil from the Gotjawal forest in Jeju, Korea.
abstract: In this study, we analyzed the bacterial diversity in soils collected from Gyorae Gotjawal forest, where globally unique topography, geology, and ecological features support a forest grown on basalt flows from 110,000 to 120,000 years ago and 40,000 to 50,000 years ago. The soils at the site are fertile, with rocky areas, and are home to endangered species of plants and animals. Rainwater penetrates to the groundwater aquifer, which is composed of 34% organic matter containing rare types of soil and no soil profile. We determined the bacterial community composition using 116,475 reads from a 454-pyrosequencing analysis. This dataset included 12,621 operational taxonomic units at 3% dissimilarity, distributed among the following groups: Proteobacteria (56.2%) with 45.7% of α-Proteobacteria, Actinobacteria (25%), Acidobacteria (10.9%), Chloroflexi (2.4%), and Bacteroidetes (0.9%). In addition, 16S rRNA gene sequences were amplified using polymerase chain reaction and domain-specific primers to construct a clone library based on 142 bacterial clones. These clones were affiliated with the following groups: Proteobacteria (56%) with 51% of α-Proteobacteria, Acidobacteria (7.8%), Actinobacteria (17.6%), Chloroflexi (2.1%), Bacilli (1.4%), Cyanobacteria (2.8%), and Planctomycetes (1.4%). Within the phylum Proteobacteria, 56 of 80 clones were tentatively identified as 12 unclassified genera. Several new genera and a new family were discovered within the Actinobacteria clones. Results from 454-pyrosequencing revealed that 57% and 34% of the sequences belonged to undescribed genera and families, respectively. The characteristics of Gotjawal soil, which are determined by lava morphology, vegetation, and groundwater penetration, might be reflected in the bacterial community composition.
authors.affiliation: Gyeongbuk Institute for Marine Bioindustry, Uljin, 767-813, Korea.
authors.forename: Jong-Shik
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Research Institute for Hallasan, Jeju Special Self-Governing Province, 690-816, Korea.
authors.forename: Dae-Shin
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Research Institute for Hallasan, Jeju Special Self-Governing Province, 690-816, Korea.
authors.forename: Suk-Hyung
authors.initials: SH
authors.lastname: Ko
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Korea.
authors.forename: Man-Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 361-763, Korea.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
authors.affiliation: Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
keywords: 16S rRNA gene; Gotjawal forest soil; bacterial diversity; pyrosequencing