title: Listeria phage and phage tail induction triggered by components of bacterial growth media (phosphate, LiCl, nalidixic acid, and acriflavine).
abstract: The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed the induction of phages and phage tails of Listeria spp. in these media and in two brain heart infusion (BHI) broths (BHIM [bioMérieux] and BHIK [Biokar]) to identify putative effectors. It appears that Na2HPO4 at concentrations ranging from 1 to 40 g/liter with an initial pH of 7.5 can induce phage or phage tail production of Listeria spp., especially with 10 g/liter of Na2HPO4 and a pH of 7.5, conditions present in half-Fraser and Fraser broths. Exposure to LiCl in BHIM (18 to 21 g/liter) can also induce phage and phage tail release, but in half-Fraser and Fraser broths, the concentration of LiCl is much lower (3 g/liter). Low phage titers were induced by acriflavine and/or nalidixic acid. We also show that the production of phages and phage tails can occur in half-Fraser and Fraser broths. This study points out that induction of phages and phage tails could be triggered by compounds present in enrichment media. This could lead to a false-negative result for the detection of L. monocytogenes in food products.
authors.affiliation: INRA, UMR1347 Agroécologie, Dijon, France Université de Bourgogne, UMR1347 Agroécologie, Dijon, France jplemait@u-bourgogne.fr.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lemaître
authors.affiliation: INRA, UMR1347 Agroécologie, Dijon, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Duroux
authors.affiliation: INRA, UMR1347 Agroécologie, Dijon, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Pimpie
authors.affiliation: Plateau Technique de Biologie, CHU Dijon, Dijon, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Duez
authors.affiliation: INRA, UMR1347 Agroécologie, Dijon, France.
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Milat
mesh_terms: D000167:Acriflavine / Q000378:metabolism; D001435:Bacteriophages / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry*; D008087:Listeria / Q000821:virology*; D018021:Lithium Chloride / Q000378:metabolism; D009268:Nalidixic Acid / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D017376:Viral Tail Proteins / Q000378:metabolism*; D014775:Virus Activation / Q000187:drug effects*
chemical_list: D003470:Culture Media; D010710:Phosphates; D017376:Viral Tail Proteins; D000167:Acriflavine; D009268:Nalidixic Acid; D018021:Lithium Chloride