title: Association of leishmaniasis with TNF alpha promoter and SLC11A1 gene polymorphisms in patients of two endemic areas in Mexico.
abstract: Some Single Nucleotide Polymorphisms (SNPs) of interleukins and other modulatory molecules of the immune response play an important role in susceptibility to infectious diseases, particularly those involving intracellular parasites. In this study, we evaluated allele, genotype and haplotype associations of two SNPs of the TNF-α promoter and seven of the SLC11A1 gene in 79 patients with localized cutaneous leishmaniasis (CL) and 15 with visceral leishmaniasis (VL), compared with 127 and 89 locality paired controls, respectively, from two endemic areas of Chiapas State, Mexico. None of the TNF-α alleles and genotypes was associated either to CL or to VL. Alleles rs2276631-C (P = 0.02; OR [95%CI] = 2.11 [1.16-3.86]) and rs2279015-G (P = 0.005; OR [95%CI] = 2.42 [1.33-4.41]) of SLC11A1, were associated with susceptibility to VL, whereas genotypes rs2276631 C/C (P = 0.003; OR [95%CI] = 2.65 [1.41-5.00]) and rs2279015 G/G (P = 0.018; OR [95%CI] = 2.05 [1.15-3.64]) were significantly increased in CL and VL patients, respectively. Complete haplotypes involved in susceptibility were CGCCGDins with VL and CGCCADins with CL. CGCCA was the minimal susceptibility haplotype for CL and CCG for VL. Our data suggest that SLC11A1 gene polymorphisms might have a relevant role in the pathology of leishmaniasis, directing towards susceptibility outcome of this disease in residents of an endemic area.
authors.affiliation: Departamento de Biología Molecular e Histocompatibilidad, Hospital General Dr. Manuel Gea González, México City, Mexico.
authors.forename: Aaxin
authors.initials: A
authors.lastname: Ortiz-Flores
authors.affiliation: Departamento de Parasitología, Instituto de Diagnóstico y Referencia Epidemiológicos, México City, Mexico.
authors.forename: Gabriela
authors.initials: G
authors.lastname: De la Rosa-López
authors.affiliation: Departamento de Biología Molecular e Histocompatibilidad, Hospital General Dr. Manuel Gea González, México City, Mexico.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Zavaleta-Villa
authors.affiliation: Departamento de Parasitología, Instituto de Diagnóstico y Referencia Epidemiológicos, México City, Mexico.
authors.forename: Susana
authors.initials: S
authors.lastname: Chávez-López
authors.affiliation: Departamento de Enfermedades Transmitidas por Vector, Secretaría de Salud de Chiapas, Tuxtla Gutiérrez, Chiapas, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Pastor-Santiago
authors.affiliation: Departamento de Parasitología, Instituto de Diagnóstico y Referencia Epidemiológicos, México City, Mexico.
authors.forename: Carmen
authors.initials: C
authors.lastname: Guzmán-Bracho
authors.affiliation: Departamento de Biología Molecular e Histocompatibilidad, Hospital General Dr. Manuel Gea González, México City, Mexico.
authors.forename: Mirza
authors.initials: M
authors.lastname: Romero-Valdovinos
authors.affiliation: Departamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, México City, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez-Hernández
authors.affiliation: Departamento de Biología Molecular e Histocompatibilidad, Hospital General Dr. Manuel Gea González, México City, Mexico. Electronic address: aolivod@yahoo.com.
authors.forename: Angélica
authors.initials: A
authors.lastname: Olivo-Díaz
mesh_terms: D016022:Case-Control Studies; D027682:Cation Transport Proteins / Q000235:genetics* / Q000276:immunology; D020022:Genetic Predisposition to Disease / Q000453:epidemiology*; D006801:Humans; D016773:Leishmaniasis, Cutaneous / Q000453:epidemiology / Q000235:genetics*; D007898:Leishmaniasis, Visceral / Q000453:epidemiology / Q000235:genetics*; D008800:Mexico / Q000453:epidemiology; D011110:Polymorphism, Genetic / Q000235:genetics / Q000276:immunology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D014409:Tumor Necrosis Factor-alpha; D000099173:Natural Resistance-associated Macrophage Protein 1; C439524:TNF protein, human
keywords: DNA typing; Genetic susceptibility; Leishmaniasis; Polymorphisms; SLC11A1; TNF-α