title: Identification of optimal protein binders through the use of large genetically encoded display libraries.
abstract: The use of large genetically encoded binder libraries in co-operation with display technologies has matured over the past 25 years, and is now one of the primary methods used for selection of protein binders. Display technology has proven to be a robust and versatile method for generating binders to almost any antigen of interest. The evolution of this technology beyond antibody phage display has opened up new aspects for the concept of designer biologics. The ability to construct large populations of eukaryotic cells, including mammalian cells, where each cell expresses an individual antibody, peptide or engineered protein has added great value in identifying binders with desired properties. Here we review the evolution of display technology and highlight how it is being used today to generate binders with exquisite specificity, selectivity, affinity and developability characteristics.
authors.affiliation: IONTAS Ltd., Babraham Research Campus, Cambridge CB22 3AT, UK. Electronic address: jmc@iontas.co.uk.
authors.forename: John
authors.initials: J
authors.lastname: McCafferty
authors.affiliation: MedImmune, Milstein Building, Granta Park, Cambridge CB21 6GH, UK.
authors.forename: Darren
authors.initials: D
authors.lastname: Schofield
mesh_terms: D001665:Binding Sites; D055808:Drug Discovery; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D015335:Molecular Probes / Q000737:chemistry; D019151:Peptide Library; D011485:Protein Binding; D015202:Protein Engineering; D057127:Single-Chain Antibodies / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry*; D014169:Transformation, Bacterial
chemical_list: D015335:Molecular Probes; D019151:Peptide Library; D057127:Single-Chain Antibodies; D054852:Small Molecule Libraries