title: Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle.
abstract: The glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) are key components in the regulation of the hypothalamic-pituitary-adrenal neuroendocrine axis and coordinate the physiological response to stress agents to reestablish homeostasis. Genetic variations of GR (NR3C1) and MR (NR3C2) genes could explain the alterations in animals to adapt to challenges, and therefore, their influence on production traits. The present study aimed to identify single-nucleotide polymorphisms (SNPs) in the bovine NR3C1 and NR3C2 genes and explore their associations to relevant traits of beef cattle production. Genotypes and phenotypes were collected from 241 male Nellore cattle (119 noncastrated and 122 castrated surgically) with an average of 24 ± 1.2 mo of age and live weight of 508 ± 39 kg. The traits evaluated were concentrations of plasma adrenocorticotropic hormone (ACTH) and cortisol, muscle glycogen and lactate content, and pH, color, cooking loss, and shear force of longissimus thoracis measured on the 1st, 7th, and 14th days postmortem. Five SNPs were identified, 2 in the NR3C1 gene and 3 in the NR3C2 gene. There was an associative relationship between the SNP NR3C1_1 g.3293A>G and postmortem plasma concentration of cortisol (P = 0.0008). The SNPs NR3C2_1 g.115T>C and NR3C2_2 g.570T>C were associated with muscle glycogen content (P = 0.0306 and P = 0.0158), postmortem plasma concentration of ACTH (P = 0.0118 and P = 0.0095), and cooking loss of the steak aged 1 d (P = 0.0398 and P = 0.0423). Haplotype analysis showed associations of GR haplotypes with postmortem plasma concentrations of cortisol and MR haplotypes with meat color, cooking losses, muscle glycogen content, and plasma concentrations of ACTH. The associations observed in the present study show that SNPs in GR and MR genes are related with changes of hypothalamic-pituitary-adrenal axis activity and metabolic profile in cattle, leading to individual variation in meat quality traits.
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil. Electronic address: mdpoleti@gmail.com.
authors.forename: M D
authors.initials: MD
authors.lastname: Poleti
authors.affiliation: Division of Medical Pharmacology, Leiden/Amsterdam Center for Drug Research, Leiden University Medical Center, Leiden, The Netherlands; Department of Psychiatry, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: R H
authors.initials: RH
authors.lastname: DeRijk
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Rosa
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil.
authors.forename: C T
authors.initials: CT
authors.lastname: Moncau
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil.
authors.forename: P S
authors.initials: PS
authors.lastname: Oliveira
authors.affiliation: Department of Animal Science, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Piracicaba, Sao Paulo 13418-900, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: Coutinho
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil.
authors.forename: J P
authors.initials: JP
authors.lastname: Eler
authors.affiliation: Department of Basic Sciences, College of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Sao Paulo 13635-900, Brazil.
authors.forename: J C C
authors.initials: JC
authors.lastname: Balieiro
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D002417:Cattle / Q000235:genetics*; D063427:Food Quality; D006003:Glycogen / Q000032:analysis*; D006239:Haplotypes; D006854:Hydrocortisone / Q000097:blood*; D008297:Male; D008460:Meat / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011965:Receptors, Glucocorticoid / Q000235:genetics; D018161:Receptors, Mineralocorticoid / Q000235:genetics; D011987:Receptors, Steroid / Q000235:genetics*
chemical_list: D011965:Receptors, Glucocorticoid; D018161:Receptors, Mineralocorticoid; D011987:Receptors, Steroid; D000324:Adrenocorticotropic Hormone; D006003:Glycogen; D006854:Hydrocortisone
keywords: Beef cattle; Cortisol; Glycogen; Hypothalamic-pituitary-adrenal axis; Single-nucleotide polymorphism