title: High-speed multi-wavelength Fresnel diffraction imaging.
abstract: We demonstrate a compact lensless microscope which can capture video-rate phase contrast images of moving objects and allows numerical scanning of the focal distance after recording. Using only an RGB-detector and illumination from a single mode fiber, diffraction patterns at three wavelengths are recorded simultaneously, enabling high-speed data collection and reconstruction of phase and amplitude. The technique is used for imaging of a moving test target, beads in a flow cell, and imaging of Caenorhabditis elegans moving in a droplet of liquid.
authors.forename: Daniel W E
authors.initials: DW
authors.lastname: Noom
authors.forename: Dirk E
authors.initials: DE
authors.lastname: Boonzajer Flaes
authors.forename: Elias
authors.initials: E
authors.lastname: Labordus
authors.forename: Kjeld S E
authors.initials: KS
authors.lastname: Eikema
authors.forename: Stefan
authors.initials: S
authors.lastname: Witte
mesh_terms: D006696:Holography / Q000379:methods*; D008029:Lighting; D008858:Microscopy, Phase-Contrast / Q000379:methods*