title: Proteomic analysis of tilapia Oreochromis niloticus Streptococcus agalactiae strains with different genotypes and serotypes.
abstract: Nine tilapia Oreochromis niloticus group B streptococcus (GBS) strains differing in serotype and genotype were selected and paired. Two-dimensional difference gel electrophoresis (2D DIGE) and matrix-assisted laser-desorption ionization time-of-flight-mass spectrometry (MALDI-TOF-MS) were used to analyse the protein profiles of the strain pairs. Forty-three proteins corresponding to 66 spots were identified, of which 35 proteins were found in the seven selected strain pairs that represented pairs differing in genotype and serotype. Among the 35 proteins, numbers of differentially expressed proteins in strains of different serotypes were greater than found in strains of different genotypes, suggesting that serotype plays a more essential role than genotype in the differential protein expression among GBS strains. No distinct pattern was found with respect to genotype and the protein expression profile of GBS strains. Several proteins were identified as surface-associated cytoplasmic proteins that possessed the typical immunity-eliciting characteristics of surface proteins. The identified proteins were found to be involved in 16 biological processes and seven Kyoto encyclopaedia of genes and genomes (KEGG) pathways. The data, for the first time, identified differentially expressed proteins in O. niloticus GBS strains of different serotypes, which play a major role in immunogenicity of O. niloticus GBS than does genotype, offering further information for design of a vaccine against O. niloticus GBS.
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: R
authors.initials: R
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: T
authors.initials: T
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: W
authors.initials: W
authors.lastname: Liang
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: H
authors.initials: H
authors.lastname: Luo
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: X
authors.initials: X
authors.lastname: Gan
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: W
authors.initials: W
authors.lastname: Huang
authors.affiliation: Institute of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530005, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: A
authors.initials: A
authors.lastname: Lei
authors.affiliation: Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021, China.
authors.forename: M
authors.initials: M
authors.lastname: Chen
keywords: 2D-DIGE; Group B; MALDI-TOF; differentially expressed proteins; immunogenicity