title: Chemical genetic screens using Arabidopsis thaliana seedlings grown on solid medium.
abstract: Genetic screening has been a powerful tool in identifying new genes in a pathway of interest (forward genetics) or attributing function to a particular gene via mutagenesis (reverse genetics). Small molecule-based chemical genetics is increasingly adapted in Arabidopsis research as a tool for similar purposes, i.e., to identify genes involved in certain biological processes and to dissect the biological roles of a gene. Chemical genetic screens have been successful in circumventing genetic redundancy to assign biological roles to a gene family as well as novel functions for well-known genes. Here, we describe how to screen Arabidopsis seedlings grown on solid medium with chemical compounds.
authors.affiliation: Department of Botany and Plant Sciences, Institute of Integrative Genome Biology, University of California, Riverside, CA, 92521, USA.
authors.forename: Thanh Theresa
authors.initials: TT
authors.lastname: Dinh
authors.forename: Xuemei
authors.initials: X
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media; D055808:Drug Discovery / Q000379:methods*; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017930:Genes, Reporter; D005820:Genetic Testing; D036226:Seedlings / Q000187:drug effects* / Q000502:physiology*; D054852:Small Molecule Libraries
chemical_list: D003470:Culture Media; D054852:Small Molecule Libraries