title: Monitoring the distribution of surfactants in the stratum corneum by combined ATR-FTIR and tape-stripping experiments.
abstract: Combined ATR-FTIR (attenuated total reflection-Fourier transform infrared) spectroscopy and tape-stripping experiments in vitro on porcine ear skin were used to investigate the spatial distribution of different surfactants in the stratum corneum (SC). To reveal a possible connection between the size of the formed micelles and skin penetration, dynamic light-scattering measurements of the aqueous surfactant solutions were also taken. Compared to an alkyl polyglycoside and sucrose laurate, a deeper skin penetration of the anionic surfactants sodium dodecyl sulfate (SDS) und sodium lauryl ether sulfate (SLES) could be related to a smaller size of the formed micelles. Beside the differences in spatial distribution, a link between the physical presence of anionic surfactants in the SC and a decrease of skin hydration was found. Furthermore, the incorporation of SDS and SLES into the SC, even after a brief, consumer-orientated washing procedure with commercially available hair shampoos, was confirmed.
authors.affiliation: Research Platform 'Characterisation of Drug Delivery Systems on Skin and Investigations of Involved Mechanisms', University of Vienna, Vienna, Austria.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Hoppel
authors.forename: Evelyn
authors.initials: E
authors.lastname: Holper
authors.forename: Dieter
authors.initials: D
authors.lastname: Baurecht
authors.forename: Claudia
authors.initials: C
authors.lastname: Valenta
mesh_terms: D000269:Adhesives; D000818:Animals; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism*; D005936:Glucans / Q000494:pharmacology*; D006203:Hair Preparations; D006801:Humans; D008823:Micelles; D012869:Skin Absorption; D012967:Sodium Dodecyl Sulfate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*; D013552:Swine
chemical_list: D000269:Adhesives; D005936:Glucans; D006203:Hair Preparations; D008823:Micelles; C558491:Plantacare 810; D013501:Surface-Active Agents; C018152:sucrose monolaurate; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; C408148:sodium laureth sulfate