title: Micro-computed tomography and histomorphometric analysis of the effects of platelet-rich fibrin on bone regeneration in the rabbit calvarium.
abstract: OBJECTIVE
  The present study aimed to investigate the effectiveness of platelet-rich fibrin (PRF) on bone regeneration when used alone or in combination with hydroxyapatite (HA)/beta-tricalcium phosphate (βTCP).
  
  
  METHODS
  In this study, 20 New Zealand white rabbits were used and four calvarial defects were prepared in each animal. PRF, Straumann(®) Bone Ceramic (SBC), or PRF+SBC was applied to the defects; one defect was left untreated as a control. Ten rabbits were sacrificed at week 4 (T1) and 10 at week 8 (T2). After micro-computed tomography (micro-CT) scanning, the samples were sent for histological and histomorphometric analysis to evaluate and compare the volume and area of regenerated bone.
  
  
  RESULTS
  Histomorphometric and micro-CT analysis showed that both PRF and SBC significantly increased bone regeneration at T1 and T2 (P<0.01). When PRF was used in combination with HA/βTCP, a further significant increase in new bone formation was observed at T1 and T2 compared with that when PRF or SBC was used alone (P<0.01).
  
  
  CONCLUSIONS
  PRF has a positive effect on bone formation when used alone and in combination with HA/βTCP.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, İstanbul, Turkey. Electronic address: ahmethuseyinacar@gmail.com.
authors.forename: Ahmet Hüseyin
authors.initials: AH
authors.lastname: Acar
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Inonu University, Malatya,Turkey.
authors.forename: Ümit
authors.initials: Ü
authors.lastname: Yolcu
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Inonu University, Malatya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gül
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Inonu University, Malatya, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Keleş
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Marmara University, İstanbul, Turkey.
authors.forename: Necip Fazıl
authors.initials: NF
authors.lastname: Erdem
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, Ankara, Turkey.
authors.forename: Sevil
authors.initials: S
authors.lastname: Altundag Kahraman
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D018786:Bone Substitutes / Q000494:pharmacology*; D005337:Fibrin / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000494:pharmacology*; D011817:Rabbits; D012886:Skull / Q000000981:diagnostic imaging* / Q000601:surgery*; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D005337:Fibrin
keywords: Beta-tricalcium phosphate; Calvarial defect; Hydroxyapatite; Platelet-rich fibrin; Rabbit