title: Quantification of plasma microRNAs in a group of healthy smokers, ex-smokers and non-smokers and correlation to biomarkers of tobacco exposure.
abstract: The stability of circulating miRNAs, their non-invasive sampling techniques and deregulation in diseases make them potential candidate biomarkers of biological effect. Here, we profiled the level of 84 plasma miRNAs in 30 smokers, 20 non-smokers and 20 ex-smokers. A robust statistical strategy was applied with replicate samples to account for reproducibility of the results. We identified differential expression of miR-124 and let-7a between the smoking and control groups. We further explored the dose-response relationship of miR-124 and let-7a with two biomarkers of tobacco exposure and found that this relationship was affected by adjustments based on age, pack-year and gender.
authors.affiliation: BAT, Group Research and Development , Southampton , UK.
authors.forename: Anisha
authors.initials: A
authors.lastname: Banerjee
authors.forename: David
authors.initials: D
authors.lastname: Waters
authors.forename: Oscar M
authors.initials: OM
authors.lastname: Camacho
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Minet
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D009538:Nicotine / Q000652:urine; D025341:Principal Component Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012907:Smoking; D014633:Valine / Q000031:analogs & derivatives / Q000032:analysis; D055815:Young Adult
chemical_list: D015415:Biomarkers; C509207:MIRN124 microRNA, human; D035683:MicroRNAs; C091192:N-(2-cyanoethyl)valine; C487931:mirnlet7 microRNA, human; D009538:Nicotine; D014633:Valine
keywords: Biomarkers; TNEQ; microRNA; plasma; smoking