title: Robust polydiacetylene-based colorimetric sensing material developed with amyloid fibrils of α-synuclein.
abstract: Robust polydiacetylene-based colorimetric sensing material has been developed with amyloid fibrils of α-synuclein in the presence of 10,12-pentacosadiynoic acid (PCDA) by taking advantage of the specific fatty acid interaction of α-synuclein and structural regularity of the self-assembled product of amyloid fibrils. PCDA facilitated not only self-oligomerization of α-synuclein but also its fibrillation into the fibrils with increased thickness. Upon UV irradiation, the PCDA-containing amyloid fibrils (AF-PCDAs) turned blue, which then became red following heat treatment. The blue-to-red color transition was also observed with other stimuli of pH and ethanol. AF-PCDAs were demonstrated to be mechanically stable since not only the individual colors of blue and red but also their colorimetric transition were not affected by a number of sonications which readily disrupted the polydiaceylene (PDA) vesicles with the instant loss of color. Therefore, AF-PCDA can be considered to be a novel PDA-based colorimetric sensing material with high mechanical strength, which has the potential to be employed in various areas involving advanced sensing technologies.
authors.affiliation: School of Chemical and Biological Engineering, Institute of Chemical Processes, College of Engineering, Seoul National University , Seoul 151-744, Korea.
authors.forename: Jee Eun
authors.initials: JE
authors.lastname: Yang
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Park
authors.forename: Eunae
authors.initials: E
authors.lastname: Cho
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
authors.forename: Seung R
authors.initials: SR
authors.lastname: Paik
mesh_terms: D000682:Amyloid / Q000737:chemistry*; D003116:Color; D003124:Colorimetry / Q000379:methods*; D000431:Ethanol / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary; D013314:Stress, Mechanical; D014466:Ultraviolet Rays; D051844:alpha-Synuclein / Q000737:chemistry*
chemical_list: C493048:10, 12-pentacosadiynoic acid; D000682:Amyloid; D005231:Fatty Acids, Unsaturated; D000078789:Polyacetylene Polymer; D011108:Polymers; D051844:alpha-Synuclein; D053279:Polyynes; C082361:polydiacetylene; D000431:Ethanol