title: Improving Leishmania species identification in different types of samples from cutaneous lesions.
abstract: The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliable protocol for species identification.
authors.affiliation: Laboratorio de Parasitología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Mónica L
authors.initials: ML
authors.lastname: Cruz-Barrera
authors.affiliation: Centro Dermatológico Federico Lleras Acosta, Bogotá, Colombia.
authors.forename: Clemencia
authors.initials: C
authors.lastname: Ovalle-Bracho
authors.affiliation: Centro Dermatológico Federico Lleras Acosta, Bogotá, Colombia.
authors.forename: Viviana
authors.initials: V
authors.lastname: Ortegon-Vergara
authors.affiliation: Hospital Militar Central, Bogotá, Colombia Departamento de Medicina Interna, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.
authors.forename: Jairo E
authors.initials: JE
authors.lastname: Pérez-Franco
authors.affiliation: Laboratorio de Parasitología, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia mcecheverryg@unal.edu.co.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Echeverry
mesh_terms: D016054:DNA, Protozoan / Q000235:genetics*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007891:Leishmania / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D013045:Species Specificity
chemical_list: D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins