title: (1)H, (15)N, (13)C resonance assignment of human osteopontin.
abstract: Osteopontin (OPN) is a 33.7 kDa intrinsically disordered protein and a member of the SIBLING family of proteins. OPN is bearing a signal peptide for secretion into the extracellular space, where it exerts its main physiological function, the control of calcium biomineralization. It is often involved in tumorigenic processes influencing proliferation, migration and survival, as well as the adhesive properties of cancer cells via CD44 and integrin signaling pathways. Here we report the nearly complete NMR chemical shift assignment of recombinant human osteopontin.
authors.affiliation: Max F. Perutz Laboratories, Department of Computational and Structural Biology, University of Vienna, Campus Vienna Biocenter 5, 1030, Vienna, Austria. gerald.platzer@univie.ac.at.
authors.forename: Gerald
authors.initials: G
authors.lastname: Platzer
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Szymon
authors.initials: S
authors.lastname: Żerko
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
authors.forename: Wiktor
authors.initials: W
authors.lastname: Koźmiński
authors.affiliation: Max F. Perutz Laboratories, Department of Computational and Structural Biology, University of Vienna, Campus Vienna Biocenter 5, 1030, Vienna, Austria. robert.konrat@univie.ac.at.
authors.forename: Robert
authors.initials: R
authors.lastname: Konrat
mesh_terms: D066241:Carbon-13 Magnetic Resonance Spectroscopy; D006801:Humans; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D053495:Osteopontin / Q000737:chemistry*; D017433:Protein Structure, Secondary; D066244:Proton Magnetic Resonance Spectroscopy
chemical_list: D009587:Nitrogen Isotopes; D053495:Osteopontin
keywords: Biomineralization; Extracellular matrix; Intrinsically disordered protein; Osteopontin