title: [Role of RAGE in lipopolysaccharide-induced cytoskeletal changes in mouse pulmonary microvascular endothelial cells].
abstract: OBJECTIVE
  To investigate lipopolysaccharide (LPS)-induced changes of cytoskeletal filamentous actin in primary isolated pulmonary microvascular endothelial cells (PMVECs) from wild-type and RAGE knock-out mouse.
  
  
  METHODS
  The lungs of wild-type and RAGE knock-out mice were digested with collagenase type I to obtain endothelial cells purified by anti-CD31-coupled magnetic beads. The PMVEC identified by factor VIII labeling were stimulated with LPS at different concentrations and the changes of filamentous actin were observed by confocal microscopy.
  
  
  RESULTS
  The cultured primary cells showed typical endothelial cell phenotype as examined with factor VIII labeling. LPS stimulation caused rearrangement of the cytoskeletal filament F-actin in wild-type mouse PMVECs with stress fiber formation, but such changes were not obvious in RAGE knock-out mouse PMVECs.
  
  
  CONCLUSIONS
  Mouse PMVECs of a high purity can be obtained by immune magnetic beads. RAGE is involved in LPS-induced destruction of mouse PMVEC cytoskeletons.
authors.affiliation: Department of Pathophysiology of Southern Medical University, Key Laboratory of Shock and Microcirculation of Guangdong Province, Guangzhou 510515, China.E-mail: 602502708@qq.com.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Wei-Jin
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Qiao-Bing
authors.initials: QB
authors.lastname: Huang
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Guo
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D042783:Endothelial Cells / Q000166:cytology*; D008070:Lipopolysaccharides; D008168:Lung / Q000166:cytology*; D051379:Mice; D018345:Mice, Knockout; D055806:Microvessels / Q000166:cytology; D010641:Phenotype; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; D008070:Lipopolysaccharides; D000067759:Receptor for Advanced Glycation End Products; D011971:Receptors, Immunologic