title: Auraptene has the inhibitory property on murine T lymphocyte activation.
abstract: Auraptene, a citrus fruit-derived coumarin, has been reported to exert valuable pharmacological properties as anti-tumor, anti-inflammatory, and anti-oxidant agent. However, little is known about auraptene on immune responses. In this study, we conducted an investigation to evaluate auraptene as an anti-T lymphocyte proliferation agent using CD3/CD28-activated lymphocytes isolated from C57BL/6 mice. We found that administration of auraptene inhibited CD3/CD28-activated lymphocyte proliferation in a dose dependent manner, but the inhibition at a wide range of doses used in this study did not induce cytotoxicity or apoptosis. In addition, auraptene dose dependently decreased the CD3/CD28-activated T lymphocyte secreting T helper (Th)1 cytokines (interleukin (IL)-2 and interferon (IFN)-γ); whereas, auraptene could decrease Th2 cytokine (IL-4) at a higher level (40µM) but had not at lower levels (10 and 20µM). Further mechanistic study demonstrated that auraptene doses dependently suppressed T cell early and middle/late activation marker CD69 and CD25 expression, respectively. Finally, auraptene could suppress cell cycle progression which contributes to inhibiting T cell proliferation and cell division. These findings indicate that auraptene exhibits anti-inflammatory properties via inhibiting T cell proliferation and their inflammatory cytokine secretion that may mediate the interaction between T cells and autoimmune disorders, suggesting that auraptene is a potential food-derived compound with a benefit to those with abnormally over-activation T cell mediated response and chronic inflammation such as autoimmune and inflammatory diseases.
authors.affiliation: College of Life Science, Henan University, Kaifeng 475004, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Niu
authors.affiliation: College of Nursing, Henan University, Kaifeng 475004, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Translational Medicine Center of Huaihe Hospital, Henan University, Kaifeng 475004, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Translational Medicine Center of Huaihe Hospital, Henan University, Kaifeng 475004, China.
authors.forename: Xuequn
authors.initials: X
authors.lastname: Ren
authors.affiliation: Translational Medicine Center of Huaihe Hospital, Henan University, Kaifeng 475004, China; Institute of Molecular Medicine, Henan University, Kaifeng 475004,China. Electronic address: jpwangchina@henu.edu.cn.
authors.forename: Junpeng
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D018106:CD28 Antigens / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003374:Coumarins / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism; D005260:Female; D008070:Lipopolysaccharides / Q000009:adverse effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; D003374:Coumarins; D016207:Cytokines; D008070:Lipopolysaccharides; C105832:aurapten
keywords: Auraptene; Cell cycle; Cytokines; Inflammation; T cells