title: Neuroprotective effects of collagen matrix in rats after traumatic brain injury.
abstract: OBJECTIVE
  In previous studies, collagen based matrices have been implanted into the site of lesion in different models of brain injury. We hypothesized that semisynthetic collagen matrix can have neuroprotective function in the setting of traumatic brain injury.
  
  
  METHODS
  Rats were subjected to sham injury or controlled cortical impact. They either received extracellular matrix graft (DuraGen) over the injury site or did not receive any graft and underwent beam balance/beam walking test at post injury days 1-5 and Morris water maze at post injury days 14-18. Animals were sacrificed at day 18 for tissue analysis.
  
  
  RESULTS
  Collagen matrix implantation in injured rats did not affect motor function (beam balance test: p = 0.627, beam walking test: p = 0.921). However, injured group with collagen matrix had significantly better spatial memory acquisition (p < 0.05). There was a significant reduction in lesion volume, as well as neuronal loss in CA1 (p < 0.001) and CA3 (p < 0.05) regions of the hippocampus in injured group with collagen matrix (p < 0.05).
  
  
  CONCLUSIONS
  Collagen matrix reduces contusional lesion volume, neuronal loss, and cognitive deficit after traumatic brain injury. Further studies are needed to demonstrate the mechanisms of neuroprotection by collagen matrix.
authors.affiliation: Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Shin
authors.affiliation: Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Grandhi
authors.affiliation: Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Henchir
authors.affiliation: Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Hong Q
authors.initials: HQ
authors.lastname: Yan
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Stephen F
authors.initials: SF
authors.lastname: Badylak
authors.affiliation: Brain Trauma Research Center, University of Pittsburgh, Pittsburgh, Pennsylvania Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania Safar Center for Resuscitation Research, University of Pittsburgh, Pittsburgh, Pennsylvania Veterans Affairs Pittsburgh Healthcare System, Pittsburgh, Pennsylvania.
authors.forename: C Edward
authors.initials: CE
authors.lastname: Dixon
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D056547:CA1 Region, Hippocampal / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D056654:CA3 Region, Hippocampal / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D003094:Collagen / Q000494:pharmacology*; D004195:Disease Models, Animal; D008297:Male; D018782:Maze Learning; D009043:Motor Activity; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D019635:Neurosurgical Procedures; D017207:Rats, Sprague-Dawley; D065852:Spatial Memory; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D018696:Neuroprotective Agents; D003094:Collagen
keywords: Collagen; bioscaffold; matrix; neuroregeneration; neurorepair; traumatic brain injury