title: Electrospun polycaprolactone matrices with tensile properties suitable for soft tissue engineering.
abstract: The extracellular environment is a complex network of functional and structural components that impart chemical and mechanical stimuli that affect cellular function and fate. Cell differentiation on three dimensional scaffolds is also determined by the modulus of the substrate. Electrospun PCL nanofibers, which mimic the extra cellular matrix, have been developed with a wide variety of solvents and their combinations. The various studies have revealed that the solvents used influence the physical and mechanical properties, resulting in scaffolds with Young's modulus in the range of 1.8-15.4 MPa, more suitable for engineering of hard tissue like bone. The current study describes the use of benign binary solvent-generated fibrous scaffolds with a Young's modulus of 36.05 ± 13.08 kPa, which is almost 50 times lower than that of scaffolds derived from the commonly used solvents, characterized with myoblast, which can be further explored for applications in muscle and soft tissue engineering.
authors.affiliation: a Cell and Tissue Engineering Laboratory, Sri Ramachandra University , Chennai , India.|f Department of Human Genetics , Sri Ramachandra University , Chennai , India.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Elamparithi
authors.affiliation: a Cell and Tissue Engineering Laboratory, Sri Ramachandra University , Chennai , India.|b Centre for Regenerative Medicine and Stem Cell Research, Sri Ramachandra University , Chennai , India.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Punnoose
authors.affiliation: a Cell and Tissue Engineering Laboratory, Sri Ramachandra University , Chennai , India.|c Department of Pathology , Sri Ramachandra Medical Centre , Chennai , India.|d Department of Pathology , the Madras Medical Mission , Chennai , India.
authors.forename: Sarah
authors.initials: S
authors.lastname: Kuruvilla
authors.affiliation: f Department of Human Genetics , Sri Ramachandra University , Chennai , India.
authors.forename: Maddaly
authors.initials: M
authors.lastname: Ravi
authors.affiliation: e Department of Periodontology , Sri Ramachandra University , Chennai , India.
authors.forename: Suresh
authors.initials: S
authors.lastname: Rao
authors.affiliation: f Department of Human Genetics , Sri Ramachandra University , Chennai , India.
authors.forename: Solomon F D
authors.initials: SF
authors.lastname: Paul
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000737:chemistry*; D032448:Myoblasts, Skeletal / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011091:Polyesters; C016240:polycaprolactone
keywords: 3D scaffolds; electrospinning; matrix modulus; polycaprolactone