title: Rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos.
abstract: Plant viruses may affect the viability and development process of their herbivore vectors. Small brown planthopper (SBPH) is main vector of Rice stripe virus (RSV), which causes serious rice stripe disease. Here, we reported the effects of RSV on SBPH offspring by crossing experiments between viruliferous and non-viruliferous strains. The life parameters of offspring from different cross combinations were compared. The hatchability of F1 progeny from viruliferous parents decreased significantly, and viruliferous rate was completely controlled by viruliferous maternal parent. To better elucidate the underlying biological mechanisms, the morphology of eggs, viral propagation and distribution in the eggs and expression profile of embryonic development genes were investigated. The results indicated that RSV replicated and accumulated in SBPH eggs resulting in developmental stunt or delay of partial eggs; in addition, RSV was only able to infect ovum but not sperm. According to the expression profile, expression of 13 developmental genes was regulated in the eggs from viruliferous parents, in which two important regulatory genes (Ls-Dorsal and Ls-CPO) were most significantly down-regulated. In general, RSV exerts an adverse effect on SBPH, which is unfavourable for the expansion of viruliferous populations. The viewpoint is also supported by systematic monitoring of SBPH viruliferous rate.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Zi
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Shangshu
authors.initials: S
authors.lastname: Ge
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Zhaobang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Deng
authors.affiliation: National University of Singapore Suzhou Research Institute, Suzhou 215123, People's Republic of China.
authors.forename: Sek-Man
authors.initials: SM
authors.lastname: Wong
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences; Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, People's Republic of China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006430:Hemiptera / Q000235:genetics / Q000254:growth & development / Q000821:virology; D019476:Insect Proteins / Q000096:biosynthesis* / Q000235:genetics; D007303:Insect Vectors / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D012275:Oryza / Q000235:genetics / Q000821:virology; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D029364:Tenuivirus / Q000235:genetics* / Q000472:pathogenicity
chemical_list: D019476:Insect Proteins