title: Effect of salivary contamination and decontamination on bond strength of two one-step self-etching adhesives to dentin of primary and permanent teeth.
abstract: OBJECTIVE
  To evaluate the effects of human saliva contamination and two decontamination procedures at different stages of the bonding procedure on the bond strength of two one-step self-etching adhesives to primary and permanent dentin.
  
  
  METHODS
  Extracted human primary and permanent molars (210 of each) were ground to mid-coronal dentin. The dentin specimens were randomly divided into 7 groups (n = 15/group/molar type) for each adhesive (Xeno V+ and Scotchbond Universal): no saliva contamination (control); saliva contamination before or after light curing of the adhesives followed by air drying, rinsing with water spray/air drying, or by rinsing with water spray/air drying/reapplication of the adhesives. Resin composite (Filtek Z250) was applied on the treated dentin surfaces. The specimens were stored at 37°C and 100% humidity for 24 h. After storage, shear bond strength (SBS) was measured and data analyzed with nonparametric ANOVA followed by exact Wilcoxon rank sum tests.
  
  
  RESULTS
  Xeno V+ generated significantly higher SBS than Scotchbond Universal when no saliva contamination occurred. Saliva contamination reduced SBS of Xeno V+, with the reduction being more pronounced when contamination occurred before light curing than after. In both situations, decontamination involving reapplication of the adhesive restored SBS. Saliva contamination had no significant effect on Scotchbond Universal. There were no differences in SBS between primary and permanent teeth.
  
  
  CONCLUSIONS
  Rinsing with water and air drying followed by reapplication of the adhesive restored bond strength to saliva-contaminated dentin.
authors.forename: Katharina
authors.initials: K
authors.lastname: Santschi
authors.forename: Anne
authors.initials: A
authors.lastname: Peutzfeldt
authors.forename: Adrian
authors.initials: A
authors.lastname: Lussi
authors.forename: Simon
authors.initials: S
authors.lastname: Flury
mesh_terms: D000268:Adhesiveness; D000388:Air; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry; D003799:Dental Stress Analysis / Q000295:instrumentation; D003804:Dentin / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D003890:Desiccation; D006801:Humans; D006813:Humidity; D055115:Light-Curing of Dental Adhesives; D008422:Materials Testing; D019279:Resin Cements / Q000737:chemistry; D012463:Saliva / Q000737:chemistry*; D033081:Shear Strength; D013314:Stress, Mechanical; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors; D014094:Tooth, Deciduous / Q000648:ultrastructure*; D014867:Water / Q000737:chemistry
chemical_list: D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D019279:Resin Cements; C575872:Xeno V; C533204:filtek Z350; D014867:Water; C041330:Scotchbond