title: Dopamine D1 and opioid receptor antagonist-induced reductions of fructose and saccharin intake in BALB/c and SWR inbred mice.
abstract: Sugar and fat intake in rodents are mediated in part by brain dopamine (DA) and opioid neurotransmitter systems although important strain differences exist. Thus, whereas sucrose intake of BALB/c and SWR mice was reduced by DA D1 (SCH23390: SCH) receptor antagonism, opioid (naltrexone: NTX) receptor antagonism reduced intake only in BALB/c mice. Both SCH and NTX reduced fat (Intralipid) intake in SWR, but not BALB/c mice. The present study extended this pharmacological analysis to caloric and non-caloric sweeteners by examining whether fructose (8%) or saccharin (0.2%) intakes were differentially suppressed in BALB/c and SWR mice by SCH (50-1600nmol/kg) or NTX (0.01-5mg/kg) over a 5- to 120-min time course. SCH significantly reduced fructose (200-1600nmol/kg) and saccharin (50-1600nmol/kg) intakes in both strains as did NTX (0.1-5mg/kg). Antagonist ID40 potencies were <50nmol/kg for SCH and 0.9mg/kg for NTX in inhibiting saccharin intake, and 1234nmol/kg for SCH and 5mg/kg for NTX in inhibiting fructose intake in BALB/c mice. For SWR mice, the ID40 potencies were <50nmol/kg for SCH and 0.02mg/kg for NTX in inhibiting saccharin intake, and 298nmol/kg for SCH and 2.6mg/kg for NTX in inhibiting fructose intake. Thus, saccharin intake was similarly reduced by SCH and NTX in BALB/c and SWR mice, but greater potencies of opioid (1.9-fold) and DA D1 (4-fold) receptor antagonism of fructose intake were observed in SWR relative to BALB/c mice, indicating strong strain differences.
authors.affiliation: Behavioral and Cognitive Neuroscience Cluster of the Psychology Doctoral Program, The Graduate Center, City University of New York, New York, NY USA.
authors.forename: Tamar T
authors.initials: TT
authors.lastname: Kraft
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Elona
authors.initials: E
authors.lastname: Natanova
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Lolier
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Yakov
authors.initials: Y
authors.lastname: Yakubov
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Sam
authors.initials: S
authors.lastname: La Magna
authors.affiliation: Department of Psychology, Queens College, City University of New York, New York, NY USA.
authors.forename: Deena
authors.initials: D
authors.lastname: Warshaw
authors.affiliation: Behavioral and Cognitive Neuroscience Cluster of the Psychology Doctoral Program, The Graduate Center, City University of New York, New York, NY USA; Department of Psychology, Brooklyn College, City University of New York, New York, NY USA.
authors.forename: Anthony
authors.initials: A
authors.lastname: Sclafani
authors.affiliation: Behavioral and Cognitive Neuroscience Cluster of the Psychology Doctoral Program, The Graduate Center, City University of New York, New York, NY USA; Department of Psychology, Queens College, City University of New York, New York, NY USA. Electronic address: richard.bodnar@qc.cuny.edu.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Bodnar
mesh_terms: D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005632:Fructose / Q000008:administration & dosage*; D008297:Male; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology*; D017447:Receptors, Dopamine D1 / Q000037:antagonists & inhibitors*; D011957:Receptors, Opioid / Q000187:drug effects; D012439:Saccharin / Q000008:administration & dosage*; D013045:Species Specificity; D013549:Sweetening Agents / Q000008:administration & dosage*
chemical_list: D001552:Benzazepines; D004040:Dietary Carbohydrates; D009292:Narcotic Antagonists; D017447:Receptors, Dopamine D1; D011957:Receptors, Opioid; C534628:SCH 23390; D013549:Sweetening Agents; D005632:Fructose; D009271:Naltrexone; D012439:Saccharin
keywords: Naltrexone; Palatability; SCH23390