title: [Cannabinoid receptor 1 controls nerve growth in ectopic cyst in a rat endometriosis model].
abstract: OBJECTIVE
  To investigate whether cannabinoid receptor 1 (CB1R) is involved in nerve growth in endometriosis-associated ectopic cyst.
  
  
  METHODS
  The effect of CB1R agonist and antagonist on the expression of pan-neuronal marker protein gene product (PGP) 9.5 in ectopic cyst was examined by immunofluorescence and Western blot in endometriosis model of 18 rats.
  
  
  RESULTS
  Immunofluorescence revealed that PGP 9.5 was expressed in the nerve fibers and was mainly distributed in the cyst hilum. Western blot revealed that the protein density of either PGP 9.5 (2 week: 0.38 ± 0.05; 4 week: 0.63 ± 0.03; 8 week: 0.80 ± 0.07, P < 0.01) or CB1R (2 week: 0.48 ± 0.04; 4 week: 0.68 ± 0.01; 8 week: 0.80 ± 0.03, P < 0.01) in the ectopic cyst increased with cyst size. In addition, compared to control group (0.75 ± 0.01), PGP 9.5 expression in the ectopic cyst was promoted by CB1R agonist ACPA (0.81 ± 0.01, P < 0.05), and inhibited by CB1R antagonist AM251 (0.67 ± 0.03, P < 0.01).
  
  
  CONCLUSIONS
  CB1R was involved in the nerve growth of ectopic cyst associated with endometriosis.
authors.affiliation: Department of Pathology, Shanghai Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201999, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Pathology, Shanghai Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201999, China.
authors.forename: Xizi
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Pathology, Shanghai Third People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201999, China. E-mail: hxhan2004@hotmail.com.
authors.forename: Hongxiu
authors.initials: H
authors.lastname: Han
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003560:Cysts / Q000378:metabolism*; D004195:Disease Models, Animal; D004715:Endometriosis / Q000378:metabolism*; D005260:Female; D010525:Peripheral Nerves / Q000254:growth & development* / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D051381:Rats; D043884:Receptor, Cannabinoid, CB1 / Q000037:antagonists & inhibitors / Q000502:physiology*; D043222:Ubiquitin Thiolesterase / Q000378:metabolism*
chemical_list: D010880:Piperidines; D011720:Pyrazoles; D043884:Receptor, Cannabinoid, CB1; C103505:AM 251; C489034:UCHL1 protein, rat; D043222:Ubiquitin Thiolesterase