title: [Clinical analysis of subpopulation of peripheral T and B lymphocytes in Chinese Parkinson's disease patients].
abstract: OBJECTIVE
  To explore the variations of subpopulation of peripheral lymphocytes in Parkinson's disease (PD) and locate its potential biomarkers for clinical evaluations.
  
  
  METHODS
  The methods of direct immunostaining and flow cytometry were employed to test the percentages of CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD19(+) lymphocytes in blood samples of 77 PD patients and 82 healthy controls. And the percentages of CD3(+)CD4(+) and CD3(+)CD8(+) lymphocytes and the parameters of patients and health controls were analyzed.
  
  
  RESULTS
  Compared to controls, the percentages of CD3(+), CD3(+)CD4(+) lymphocytes significantly decreased in PD patients ((62 ± 12)% vs (66 ± 9) %, P = 0.04; (35 ± 9) % vs (38 ± 7) %, P = 0.02), especially in males ((66 ± 9)% vs (61 ± 13) %, P = 0.02; (38 ± 10) % vs (33 ± 9) %, P = 0.01)) . Furthermore, the percentage of CD3(+) lymphocytes had a positive correlation with the course of PD in male patients (r = 0.329, P = 0.013, β = 1.423). And a negative correlation existed between the percentage of CD3(+)CD4(+) lymphocytes and the course of PD and there was a positive correlation with NMSS scale in female PD patients (r = -0.309, P = 0.045, β = -0.354; r = 0.370, P = 0.020, β = 0.486).
  
  
  CONCLUSIONS
  The variants in subpopulation of peripheral lymphocytes in PD patients may serve as a potential biomarker for diagnosing PD and predicting its clinical course.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China.
authors.forename: Siyun
authors.initials: S
authors.lastname: Zhang
authors.forename: Congcong
authors.initials: C
authors.lastname: Sun
authors.forename: Limin
authors.initials: L
authors.lastname: Zhang
authors.forename: Luan
authors.initials: L
authors.lastname: Cen
authors.forename: Mingshu
authors.initials: M
authors.lastname: Mo
authors.forename: Zhuolin
authors.initials: Z
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Feiqi
authors.initials: F
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Kang
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518036, China. Email: yilitj@hotmail.com.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: Email: pingyixujd@163.com.
authors.forename: Pingyi
authors.initials: P
authors.lastname: Xu
mesh_terms: D001402:B-Lymphocytes; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D010300:Parkinson Disease