title: [Uterine autologous transplantation in cynomolgus monkeys: a preliminary report of 6 case].
abstract: OBJECTIVE
  To evaluate the surgical feasibility of uterine autologous transplantation in female cynomolgus monkeys and explore the effect of microsurgical technique.
  
  
  METHODS
  From May 2011 to March 2014, 6 female cynomolgus monkeys, aged 7 to 12 years, were randomly divided into 2 surgical groups. In group A, gross surgeries were performed with naked eyes. In group B, uterine re-transplantation was performed under 10-time-magnifying microscopy. Anatomical data and operative durations were recorded and analyzed. Viable uterine tissue and vascular patency were observed on trans-abdominal ultrasonography and second-look laparotomy after 3 months.
  
  
  RESULTS
  The average uterus retrieval time, average vascular anastomotic time, average perfusion time and average total operative time of group B were 88.7, 147.3 and 320.0 min versus 123.7, 180.7 and 393.7 min in group A. The average perfusion durations were 35.0 and 27.3 min. And there was no inter-group difference. A total of 12 successful vascular procedures (including 6 internal iliac arteries and 6 uterine-ovary veins) of vascular anastomosis were recorded in group B versus 4 cases (including 1 artery and 3 veins) in group A (vein, P > 0.05; artery, P < 0.05). In group A, there was only 1 successful operation but uterus turned necrotic after 4 weeks. In group B, the surgical success rate was 100%. However one animal died due to intestinal obstruction. And in another 2 animals, viable uterine tissue and vascular patency were observed on trans-abdominal ultrasonography and second-look laparotomy. Two survivors resumed cyclicity at days 17 and 50 respectively as a sign of re-established uterine function.
  
  
  CONCLUSIONS
  This study has demonstrated the feasibility of uterus transplantation by vascular anastomosis in cynomolgus monkeys. And assistance of microsurgical technique can significantly improve the success rate of arterial anastomosis during uterus transplantation.
authors.affiliation: Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. Email: wyf1988@163.com.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Ping
authors.initials: P
authors.lastname: Yu
authors.forename: Gaowen
authors.initials: G
authors.lastname: Chen
authors.forename: Baota
authors.initials: B
authors.lastname: Cai
authors.forename: Zhengguang
authors.initials: Z
authors.lastname: Zhang
authors.forename: Na
authors.initials: N
authors.lastname: Liu
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Lü
authors.forename: Juxiang
authors.initials: J
authors.lastname: Xiong
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhong
authors.forename: Junhua
authors.initials: J
authors.lastname: Rao
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal; D001158:Arteries; D005260:Female; D007083:Iliac Artery; D008252:Macaca fascicularis; D010053:Ovary; D010477:Perfusion; D014182:Transplantation, Autologous; D014599:Uterus; D014656:Vascular Surgical Procedures; D014680:Veins