title: Tween 20-stabilized gold nanoparticles combined with adenosine triphosphate-BODIPY conjugates for the fluorescence detection of adenosine with more than 1000-fold selectivity.
abstract: This study describes the development of a simple, enzyme-free, label-free, sensitive, and selective system for detecting adenosine based on the use of Tween 20-stabilized gold nanoparticles (Tween 20-AuNPs) as an efficient fluorescence quencher for boron dipyrromethene-conjugated adenosine 5'-triphosphate (BODIPY-ATP) and as a recognition element for adenosine. BODIPY-ATP can interact with Tween 20-AuNPs through the coordination between the adenine group of BODIPY-ATP and Au atoms on the NP surface, thereby causing the fluorescence quenching of BODIPY-ATP through the nanometal surface energy transfer (NSET) effect. When adenosine attaches to the NP surface, the attached adenosine exhibits additional electrostatic attraction to BODIPY-ATP. As a result, the presence of adenosine enhances the efficiency of AuNPs in fluorescence quenching of BODIPY-ATP. The AuNP-induced fluorescence quenching of BODIPY-ATP progressively increased with an increase in the concentration of adenosine; the detection limit at a signal-to-noise ratio of 3 for adenosine was determined to be 60nM. The selectivity of the proposed system was more than 1000-fold for adenosine over any adenosine analogs and other nucleotides. The proposed system combined with a phenylboronic acid-containing column was successfully applied to the determination of adenosine in urine.
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Taiwan.
authors.forename: Szu-Ying
authors.initials: SY
authors.lastname: Hung
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Taiwan.
authors.forename: Ya-Chen
authors.initials: YC
authors.lastname: Shih
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-sen University, Taiwan; Center for Stem Cell Research, Kaohsiung Medical University, Taiwan. Electronic address: tsengwl@mail.nsysu.edu.tw.
authors.forename: Wei-Lung
authors.initials: WL
authors.lastname: Tseng
mesh_terms: D000241:Adenosine / Q000032:analysis* / Q000652:urine; D000255:Adenosine Triphosphate / Q000737:chemistry*; D000328:Adult; D001896:Boron Compounds / Q000737:chemistry*; D005260:Female; D005456:Fluorescent Dyes; D006046:Gold; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D012680:Sensitivity and Specificity; D059629:Signal-To-Noise Ratio; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D011136:Polysorbates; D006046:Gold; D000255:Adenosine Triphosphate; D000241:Adenosine
keywords: Adenosine; Fluorescence; Nanoparticles; Sensor