title: Effect of synthetic colloid administration on coagulation in healthy dogs and dogs with systemic inflammation.
abstract: BACKGROUND
  Synthetic colloids are often used during fluid resuscitation and affect coagulation.
  
  
  OBJECTIVE
  To compare the effects of an isotonic crystalloid and synthetic colloid on coagulation in healthy dogs and dogs with systemic inflammation.
  
  
  METHODS
  Sixteen adult purpose-bred Beagles.
  
  
  METHODS
  Randomized, placebo-controlled, blinded study. Dogs were randomized into one of two groups receiving fluid resuscitation with either 40 mL/kg IV 0.9% NaCl or tetrastarch after administration of lipopolysaccharide or an equal volume of placebo. After a 14-day washout period, the study was repeated such that dogs received the opposite treatment (LPS or placebo) but the same resuscitation fluid. Blood samples were collected at 0, 1, 2, 4, and 24 hours for measurement of coagulation variables.
  
  
  RESULTS
  Administration of either fluid to healthy dogs and dogs with systemic inflammation resulted in similar increases in prothrombin time and activated clotting time. In comparison to saline administration, tetrastarch administration resulted in significantly decreased R (P = .017) in healthy dogs, as well as significantly increased activated partial thromboplastin time (P ≤ .016), CL30% (P ≤ .016), and K (P < .001) and significantly decreased platelet count (P = .019), α (P ≤ .001), MA (P < .001), and von Willebrand factor antigen (P < .001) and collagen binding activity (P ≤ .003) in both healthy dogs and dogs with systemic inflammation.
  
  
  CONCLUSIONS
  Tetrastarch bolus administration to dogs with systemic inflammation resulted in a transient hypocoagulability characterized by a prolonged activated partial thromboplastin time, decreased clot formation speed and clot strength, and acquired type 1 von Willebrand disease.
authors.affiliation: Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Gauthier
authors.forename: M K
authors.initials: MK
authors.lastname: Holowaychuk
authors.forename: C L
authors.initials: CL
authors.lastname: Kerr
authors.forename: A M E
authors.initials: AM
authors.lastname: Bersenas
authors.forename: R Darren
authors.initials: RD
authors.lastname: Wood
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D003102:Colloids / Q000627:therapeutic use*; D004283:Dog Diseases / Q000139:chemically induced / Q000188:drug therapy*; D004285:Dogs; D005260:Female; D005440:Fluid Therapy / Q000662:veterinary; D007249:Inflammation / Q000139:chemically induced / Q000662:veterinary*; D007552:Isotonic Solutions / Q000627:therapeutic use; D008070:Lipopolysaccharides / Q000633:toxicity; D010952:Plasma Substitutes / Q000627:therapeutic use; D012151:Resuscitation / Q000662:veterinary
chemical_list: D003102:Colloids; D007552:Isotonic Solutions; D008070:Lipopolysaccharides; D010952:Plasma Substitutes
keywords: Lipopolysaccharide; Tetrastarch; Thromboelastography; von Willebrand factor