title: Genetic polymorphism of the human organic solute carrier protein 1 (hOSCP1) gene in Japanese patients with non-viral liver carcinoma.
abstract: Human organic solute carrier protein 1 (hOSCP1) is a Na(+)-independent multispecific organic solute transporter. To date, several studies have revealed that gene mutations of the transporters are likely to be associated with some diseases; however, there are no data concerning the genetic polymorphism of the hOSCP1 gene in Japanese patients with non-viral liver carcinoma (LC). In the present study, we isolated genomic DNA from a normal portion of LC, and analyzed 41 single nucleotide polymorphisms (SNPs) chosen from a database of SNPs (dbSNPs). We found genotype frequencies for 2 non-synonymous SNPs [rs34409118 (Thr(131) → Ala) and rs1416840 (Ile(219) → Thr)] and 1 synonymous SNP [rs16822954 (Ser(193) → Ser)] to be statistically significant when compared with dbSNPs. No statistical significance was observed in rs2275477 (Gly(307) → Arg) in the hOSCP1 gene. With respect to the allele frequency, we also observed rs34409118 to be statistically significant. Interestingly, we found that non-viral LC patients do not carry heterozygous mutations in rs1416840 (A/G) and rs16822954 (A/G), suggesting that a non-carrier of heterozygous mutations in these two SNPs might be a biomarker for susceptibility for non-viral LC in Japanese. Further analyses of patients with hOSCP1 variants may elucidate the relationship between the hOSCP1 gene and susceptibility of non-viral LC in Japanese patients.
authors.affiliation: Department of Pharmacotherapeutics, Division of Clinical Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Toda
authors.affiliation: Department of Pharmacotherapeutics, Division of Clinical Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Yasuna
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: School of Medicine, Department of Gastroenterological and General Surgery, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Tomotake
authors.initials: T
authors.lastname: Koizumi
authors.affiliation: School of Medicine, Department of Pathology, Division of Pathology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Saito
authors.affiliation: Department of Pharmacotherapeutics, Division of Clinical Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ohbayashi
authors.affiliation: Department of Pharmacotherapeutics, Division of Clinical Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Kohyama
authors.affiliation: School of Medicine, Department of Gastroenterological and General Surgery, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Aoki
authors.affiliation: School of Medicine, Department of Gastroenterological and General Surgery, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Murakami
authors.affiliation: School of Pharmacy, 2nd Department of Pharmacology, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Yasuhara
authors.affiliation: Department of Pharmacotherapeutics, Division of Clinical Pharmacy, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Yamamoto
keywords: AGC2, aspartate glutamate carrier 2; ALT, alanine aminotransferase; AST, aspartate aminotransferase; DNA, deoxyribonucleic acid; Genetic polymorphism; HCC, hepatocellular carcinoma; HCV, hepatitis C virus; HWE, Hardy–Weinberg equilibrium; ICC, intrahepatic cholangiocarcinoma; ICG, indocyanine green test; LC, liver carcinoma; LDH, lactate dehydrogenase; MDR1, multidrug-resistance 1; NAFLD, non-alcoholic fatty liver disease; Non-viral liver carcinoma; OAT, organic anion transporter; OATP, organic anion transporting polypeptide; PCR, polymerase chain reaction; SLC/Slc, solute carrier; SNPs, single nucleotide polymorphisms; Transporter; cSNPs, coding single nucleotide polymorphisms; hOSCP1; hOSCP1, human organic solute carrier protein 1; hURAT1, urate transporter 1; γ-GTP, γ-glutamyltranspeptidase