title: Ultrasound-assisted aqueous two-phase extraction of phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems.
abstract: An efficient ultrasound-assisted aqueous two-phase extraction and enrichment process for phenylethanoid glycosides from Cistanche deserticola Y. C. Ma stems was developed in this work. An ethanol/ammonium sulfate system was chosen for the aqueous two-phase system due to its fine partitioning and recycling behaviors. Single-factor experiments and response surface methodology were used to optimize the process parameters of the ultrasound-assisted aqueous two-phase extraction. The optimal conditions were as follows: a salt concentration of 23.5%, an ethanol concentration of 20%, an extraction time of 37 min, an extraction temperature of 30°C, a liquid/solid ratio of 30:1 w/w, and an ultrasound power of 300 W. Under the above conditions, the extraction yields of echinacoside and acteoside (the main components of phenylethanoid glycosides) reached 5.35 and 6.22 mg/g dry material weight, respectively. The contents of echinacoside and acteoside in the extracts reached 27.56 and 30.23 mg/g, respectively, which were 2.46- and 2.58-fold higher than the amounts obtained in ultrasound-assisted extraction. In conclusion, ultrasound-assisted aqueous two-phase extraction was an efficient, ecofriendly, and economical method, and it may be a promising technique for extracting and enriching bioactive components from plants.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR China; School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, PR China.
authors.forename: Beitao
authors.initials: B
authors.lastname: Dong
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Yuan
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Zhao
authors.forename: Qingming
authors.initials: Q
authors.lastname: Feng
authors.forename: Boyan
authors.initials: B
authors.lastname: Liu
authors.forename: Yuanheng
authors.initials: Y
authors.lastname: Guo
authors.forename: Bing
authors.initials: B
authors.lastname: Zhao
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031671:Cistanche / Q000737:chemistry*; D006027:Glycosides / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D013696:Temperature; D014465:Ultrasonics; D014867:Water
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D014867:Water
keywords: Acteosides; Cistanche deserticola; Echinacosides; Phenylethanoid glycosides