title: Genomic analyses of metal resistance genes in three plant growth promoting bacteria of legume plants in Northwest mine tailings, China.
abstract: To better understand the diversity of metal resistance genetic determinant from microbes that survived at metal tailings in northwest of China, a highly elevated level of heavy metal containing region, genomic analyses was conducted using genome sequence of three native metal-resistant plant growth promoting bacteria (PGPB). It shows that: Mesorhizobium amorphae CCNWGS0123 contains metal transporters from P-type ATPase, CDF (Cation Diffusion Facilitator), HupE/UreJ and CHR (chromate ion transporter) family involved in copper, zinc, nickel as well as chromate resistance and homeostasis. Meanwhile, the putative CopA/CueO system is expected to mediate copper resistance in Sinorhizobium meliloti CCNWSX0020 while ZntA transporter, assisted with putative CzcD, determines zinc tolerance in Agrobacterium tumefaciens CCNWGS0286. The greenhouse experiment provides the consistent evidence of the plant growth promoting effects of these microbes on their hosts by nitrogen fixation and/or indoleacetic acid (IAA) secretion, indicating a potential in-site phytoremediation usage in the mining tailing regions of China.
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China; Molecular Microbiology, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), 06120, Germany. Electronic address: xiepin1985@126.com.
authors.forename: Pin
authors.initials: P
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiuli
authors.initials: X
authors.lastname: Hao
authors.affiliation: Molecular Microbiology, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), 06120, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Herzberg
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Molecular Microbiology, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), 06120, Germany.
authors.forename: Dietrich H
authors.initials: DH
authors.lastname: Nies
authors.affiliation: State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: weigehong@nwsuaf.edu.cn.
authors.forename: Gehong
authors.initials: G
authors.lastname: Wei
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002681:China; D007887:Fabaceae / Q000382:microbiology*; D029902:Medicago / Q000254:growth & development / Q000382:microbiology; D059128:Mesorhizobium / Q000235:genetics* / Q000502:physiology; D019216:Metals, Heavy / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000382:microbiology; D017434:Protein Structure, Tertiary; D031301:Robinia / Q000254:growth & development / Q000382:microbiology; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000502:physiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D019216:Metals, Heavy; D012989:Soil Pollutants
keywords: Legume–rhizobia symbiosis; Metal resistance gene; Plant growth promoting bacteria