title: Therapeutic effect of nerve growth factor on cerebral infarction in dogs using the hemisphere anomalous volume ratio of diffusion-weighted magnetic resonance imaging.
abstract: A model of focal cerebral ischemic infarction was established in dogs through middle cerebral artery occlusion of the right side. Thirty minutes after occlusion, models were injected with nerve growth factor adjacent to the infarct locus. The therapeutic effect of nerve growth factor against cerebral infarction was assessed using the hemisphere anomalous volume ratio, a quantitative index of diffusion-weighted MRI. At 6 hours, 24 hours, 7 days and 3 months after modeling, the hemisphere anomalous volume ratio was significantly reduced after treatment with nerve growth factor. Hematoxylin-eosin staining, immunohistochemistry, electron microscopy and neurological function scores showed that infarct defects were slightly reduced and neurological function significantly improved after nerve growth factor treatment. This result was consistent with diffusion-weighted MRI measurements. Experimental findings indicate that nerve growth factor can protect against cerebral infarction, and that the hemisphere anomalous volume ratio of diffusion-weighted MRI can be used to evaluate the therapeutic effect.
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Radiology, Hebei General Hospital, Shijiazhuang 050051, Hebei Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Computer Science, Institute of Basic Medicine, Hebei Medical University, Shijiazhuang 050031, Hebei Province, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Zuojun
authors.initials: Z
authors.lastname: Geng
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Huaijun
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Haiqing
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Song
authors.affiliation: Department of Radiology, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
keywords: brain; cerebral infarction; diffusion-weighted MRI; hemisphere anomalous volume ratio; nerve growth factor; neural regeneration; neuroprotection; regeneration; treatment