title: NMR resonance assignments of the lantibiotic immunity protein NisI from Lactococcus lactis.
abstract: The lantibiotic nisin is a small antimicrobial peptide which acts against a wide range of Gram-positive bacteria. Nisin-producing Lactococcus lactis strains express four genes for self-protection against their own antimicrobial compound. This immunity system consists of the lipoprotein NisI and the ABC transporter NisFEG. NisI is attached to the outside of the cytoplasmic membrane via a covalently linked diacylglycerol anchor. Both the lipoprotein and the ABC transporter are needed for full immunity but the exact immunity mechanism is still unclear. To gain insights into the highly specific immunity mechanism of nisin producing strains on a structural level we present here the backbone resonance assignment of NisI (25.8 kDa) as well as the virtually complete (1)H,(15)N,(13)C chemical shift assignments for the isolated 12.7 kDa N-terminal and 14.6 kDa C-terminal domains of NisI.
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-Universität Frankfurt/M., 60438, Frankfurt, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Hacker
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-Universität Frankfurt/M., 60438, Frankfurt, Germany.
authors.forename: Nina Alexandra
authors.initials: NA
authors.lastname: Christ
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-Universität Frankfurt/M., 60438, Frankfurt, Germany.
authors.forename: Elke
authors.initials: E
authors.lastname: Duchardt-Ferner
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Sophie
authors.initials: S
authors.lastname: Korn
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Lucija
authors.initials: L
authors.lastname: Berninger
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Kötter
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.
authors.forename: Karl-Dieter
authors.initials: KD
authors.lastname: Entian
authors.affiliation: Institut für Molekulare Biowissenschaften, Johann-Wolfgang-Goethe-Universität Frankfurt/M., Max-von-Laue-Str. 9, 60438, Frankfurt, Germany. woehnert@bio.uni-frankfurt.de.|Center for Biomolecular Magnetic Resonance (BMRZ), Johann-Wolfgang-Goethe-Universität Frankfurt/M., 60438, Frankfurt, Germany. woehnert@bio.uni-frankfurt.de.
authors.forename: Jens
authors.initials: J
authors.lastname: Wöhnert
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001430:Bacteriocins / Q000737:chemistry*; D007109:Immunity; D013294:Lactococcus lactis / Q000378:metabolism*; D008074:Lipoproteins / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D008074:Lipoproteins; D008565:Membrane Proteins; C094909:NisI protein, Lactococcus lactis
keywords: Lanthipeptide; Lantibiotic; Lantibiotic immunity; NMR-assignments; NisI; Nisin; Triple resonance experiments