title: [Ursodeoxycholic acid induced apoptosis of human hepatoma cells HepG2 and SMMC-7721 bymitochondrial-mediated pathway].
abstract: OBJECTIVE
  To explore the effects and underlying mechanisms of ursodeoxycholic acid on human hepatoma cells.
  
  
  METHODS
  HepG2 and SMMC-7721 HCC cell lines were respectively treated with ursodeoxycholic acid. And cell proliferation, apoptosis and the expression of Bax/Bcl-2 gene were detected by methyl thiazolyl tetrazolium (MTT), inverted microscopy, fluorescent microscopy, flow cytometry and Western blot.
  
  
  RESULTS
  Ursodeoxycholic acid significantly inhibited the proliferation of human hepatoma cells in a concentration- and time-dependent manner. The half maximal inhibitory concentrations (IC50) of HepG2 and SMMC-7721 were 397.3 and 387.7 µg/ml respectively after a 48-hour treatment of 400 µg /ml ursodeoxycholic acid. And it also induced the apoptosis of HepG2 and SMMC-7721 cells, up-regulated Bax gene and down-regulated Bcl-2 gene.
  
  
  CONCLUSIONS
  Ursodeoxycholic acid inhibits the proliferation of hepatoma cells and induce apoptosis by mitochondrial-mediated pathway.
authors.affiliation: Department of Surgery, Peking 401 Hospital of China Nuclear Industry, Beijing 102413, China.
authors.forename: Duan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Hepatobiliary Surgery, Affiliated Zhongshan Hospital, Xiamen University, Diagnosis & Treatment of Digestive Diseases Center, Fujian Provincial Key Laboratory of Chronic Liver Disease & Hepatocellular Carcinoma, Xiamen, Fujian 361004, China. Email:zhoujianyin2000@sina.com.
authors.forename: Jianyin
authors.initials: J
authors.lastname: Zhou
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Yin
authors.forename: Pingguo
authors.initials: P
authors.lastname: Liu
authors.forename: Yilin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jianming
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D008113:Liver Neoplasms; D008928:Mitochondria; D015854:Up-Regulation; D014580:Ursodeoxycholic Acid
chemical_list: D014580:Ursodeoxycholic Acid