title: The therapeutic impact of HNP-1 in condyloma acuminatum.
abstract: BACKGROUND
  Condyloma acuminatum is one of the most commonly occurring sexually transmitted diseases. HNP1 is a small antimicrobial peptide that has been reported to have antiviral activities.
  
  
  OBJECTIVE
  Using the condyloma acuminatum tissue culture to resemble the situation more closely in vivo, we investigate the therapeutic effect of a recombinant plasmid encoding HNP1 gene in condyloma acuminatum tissue.
  
  
  METHODS
  Recombinant plasmid DNA carrying HNP1 cDNA was constructed and identified. Then the recombinant plasmid was transfected into a condyloma acuminatum tissue fragment, and the HNP1 expression was determined on these tissue fragments by immunohistochemistry. TUNEL staining and flow cytometry techniques were used to examine cell apoptosis of condyloma acuminatum tissue. Relative real-time polymerase chain reaction was used to validate antihuman papillomavirus therapeutics of the treatment groups.
  
  
  RESULTS
  Transfected HNP1 gene was expressed mainly in the cytoplasmic granules of the condyloma acuminatum tissues. Positive apoptotic cells were observed in condyloma acuminatum tissues transfected with the HNP1 gene. In addition, the HPV expression was lower in the HNP1 treatment tissues as compared to their corresponding control tissues.
  
  
  CONCLUSIONS
  The results indicate that HNP1 can directly promote condyloma acuminatum cell apoptosis and play an antivirus role in the condyloma acuminatum tissue by limiting viral replication. These observations suggest a possible application for human HNP1 on condyloma acuminatum therapy.
authors.affiliation: Biotherapy Laboratory of Gynecological Oncology, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Anatomy, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Hong Ying
authors.initials: HY
authors.lastname: Zhou
authors.affiliation: Biotherapy Laboratory of Gynecological Oncology, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Li
authors.affiliation: Biotherapy Laboratory of Gynecological Oncology, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.affiliation: Biotherapy Laboratory of Gynecological Oncology, Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis / Q000235:genetics; D003218:Condylomata Acuminata / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D003594:Cytoplasmic Granules / Q000737:chemistry; D005260:Female; D015316:Genetic Therapy; D052140:Human papillomavirus 11 / Q000502:physiology; D006801:Humans; D010957:Plasmids; D012871:Skin Diseases / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D046509:Tissue Culture Techniques; D014162:Transfection; D014779:Virus Replication; D023084:alpha-Defensins / Q000032:analysis / Q000235:genetics*
chemical_list: D023084:alpha-Defensins; C406413:human neutrophil peptide 1