title: Detection of biomarkers for inflammatory diseases by an electrochemical immunoassay: the case of neopterin.
abstract: An electrochemical immunoassay for neopterin was developed using recently produced specific antibodies immobilized to protein A-coated magnetic beads in combination with differential pulse voltammetry and screen-printed array of electrodes. Neopterin-alkaline phosphatase conjugate was used as label in a competitive assay format. Multiplexed analysis of neopterin was demonstrated by replacing the traditional ELISA with electrochemical detection and the traditional plastic wells with screen-printed array of electrodes. The optimized electrochemical method, based on polyclonal antibodies, reached a limit of detection of 0.008 ng/mL with an average RSD %=10. Serum samples collected from patients with sepsis, healthy volunteers and other patients without a confirmed clinical diagnosis were also analyzed. The obtained results, compared with those of a commercial ELISA kit, had a significant correlation, showing the possibility to distinguish among the serum samples from ill or healthy subjects.
authors.affiliation: Università degli Studi di Firenze, Dipartimento di Scienze Biomediche, Sperimentali e Cliniche, Viale G. Pieraccini 6, 50139 Firenze, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Centi
authors.affiliation: Istituto di Fisica Applicata "Nello Carrara", Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, 50019 Sesto Fiorentino, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Tombelli
authors.affiliation: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors.forename: Mariarita
authors.initials: M
authors.lastname: Puntoni
authors.affiliation: Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Via G. Moruzzi 1, 56124 Pisa, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Domenici
authors.affiliation: Department of Biotechnology, Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic.
authors.forename: Milan
authors.initials: M
authors.lastname: Franek
authors.affiliation: Università degli Studi di Firenze, Dipartimento di Chimica, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy; Istituto di Biochimica delle Proteine, Via P. Castellino 111, 80131 Napoli, Italy. Electronic address: ilaria.palchetti@unifi.it.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Palchetti
mesh_terms: D000906:Antibodies / Q000276:immunology; D015415:Biomarkers; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D004566:Electrodes; D006801:Humans; D055767:Immobilized Proteins / Q000276:immunology; D007118:Immunoassay; D007249:Inflammation; D019798:Neopterin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D018805:Sepsis / Q000097:blood
chemical_list: D000906:Antibodies; D015415:Biomarkers; D055767:Immobilized Proteins; D019798:Neopterin
keywords: Electrochemical immunoassay; Magnetic beads; Neopterin; Screen-printed arrays; Serum samples