title: Analysis of diffraction imaging in non-conjugate configurations.
abstract: Diffraction imaging of scattered light allows extraction of information on scatterer's morphology. We present a method for accurate simulation of diffraction imaging of single particles by combining rigorous light scattering model with ray-tracing software. The new method has been validated by comparison to measured images of single microspheres. Dependence of fringe patterns on translation of an objective based imager to off-focus positions has been analyzed to clearly understand diffraction imaging with multiple optical elements. The calculated and measured results establish unambiguously that diffraction imaging should be pursued in non-conjugate configurations to ensure accurate sampling of coherent light distribution from the scatterer.
authors.forename: Ran
authors.initials: R
authors.lastname: Pan
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Feng
authors.forename: Yu
authors.initials: Y
authors.lastname: Sa
authors.forename: Jun Q
authors.initials: JQ
authors.lastname: Lu
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Jacobs
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Hu