title: Single-cell analysis of G-protein signal transduction.
abstract: The growing use of fluorescent biosensors to directly probe the spatiotemporal dynamics of biochemical processes in living cells has revolutionized the study of intracellular signaling. In this review, we summarize recent developments in the use of biosensors to illuminate the molecular details of G-protein-coupled receptor (GPCR) signaling pathways, which have long served as the model for our understanding of signal transduction, while also offering our perspectives on the future of this exciting field. Specifically, we highlight several ways in which biosensor-based single-cell analyses are being used to unravel many of the enduring mysteries that surround these diverse signaling pathways.
authors.affiliation: From the Department of Pharmacology and Molecular Sciences and.
authors.forename: Terri
authors.initials: T
authors.lastname: Clister
authors.affiliation: From the Department of Pharmacology and Molecular Sciences and.
authors.forename: Sohum
authors.initials: S
authors.lastname: Mehta
authors.affiliation: From the Department of Pharmacology and Molecular Sciences and The Solomon H. Snyder Department of Neuroscience and Department of Oncology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 jzhang32@jhmi.edu.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer / Q000295:instrumentation / Q000379:methods; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D015398:Signal Transduction; D059010:Single-Cell Analysis / Q000295:instrumentation / Q000379:methods*
chemical_list: D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins
keywords: Biosensor; Cell Signaling; Fluorescence Resonance Energy Transfer (FRET); G Protein; Live-cell Imaging; Signal Transduction