title: Characterization and tissue incorporation of cross-linked human acellular dermal matrix.
abstract: Here, we describe a novel human acellular dermal matrix (ADM) cross-linked using electron beam irradiation. Structural and biomechanical characteristics of the human ADM were assessed by infrared spectrometry and uni-axial tensile testing. Electron beam irradiation affects collagen secondary structure, which can be detected in the amide I spectral region (1660 cm(-1) and 1690 cm(-1)). At doses exceeding 25 kGy, cross-linking of the collagen matrix results in a denser, more stratified appearance and parallel arrangement, with significantly increased tensile strength and elastic modulus. In a micropig model, the implanted ADM elicits rapid host cell infiltration and extracellular matrix deposition; however, the delayed remodeling resulted in long-term structural integrity. Furthermore, mean densities of collagen and elastin, expression of extracellular matrix proteins, and microvessel formation within the implanted ADM increased significantly, whereas the thickness of the implanted ADM did not decrease during the course of the study. Compared with normal adjacent tissue, type I collagen mRNA levels in the ADM increased 12-fold at 3 months after implantation, and transforming growth factor-β mRNA levels increased 3.3-fold at 2 months. Matrix metalloproteinase (MMP)-1 and MMP-9 mRNA levels were also elevated. Collectively, these results demonstrate that the structural and biomechanical properties of this novel cross-linked human ADM are adequate for use as a biologic tissue substitute.
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea. Electronic address: juhee@yuhs.ac.
authors.forename: Ju Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: L&C BIO Co./R&D Center, SK Techno Park Bizcenter 2F 201, 190-2, Sangdaewon-dong, Joongwon-gu, Seongnam-Si, Gyeonggi-do, Republic of Korea. Electronic address: harrykim@lncbio.co.kr.
authors.forename: Hyung Goo
authors.initials: HG
authors.lastname: Kim
authors.affiliation: Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, Republic of Korea. Electronic address: pswjlee@yuhs.ac.
authors.forename: Won Jai
authors.initials: WJ
authors.lastname: Lee
mesh_terms: D062088:Acellular Dermis; D000818:Animals; D002196:Capillaries / Q000187:drug effects / Q000254:growth & development; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects; D017550:Spectroscopy, Fourier Transform Infrared; D034421:Sus scrofa; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects
chemical_list: D003432:Cross-Linking Reagents
keywords: Acellular dermal matrix; Biomechanical property; Cross-linked; Micropig implantation