title: Advanced methods of microscope control using μManager software.
abstract: μManager is an open-source, cross-platform desktop application, to control a wide variety of motorized microscopes, scientific cameras, stages, illuminators, and other microscope accessories. Since its inception in 2005, μManager has grown to support a wide range of microscopy hardware and is now used by thousands of researchers around the world. The application provides a mature graphical user interface and offers open programming interfaces to facilitate plugins and scripts. Here, we present a guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Arthur D
authors.initials: AD
authors.lastname: Edelstein
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Tsuchida
authors.affiliation: Luminous Point, LLC, 626 Prentiss St, San Francisco, CA 94110, USA.
authors.forename: Nenad
authors.initials: N
authors.lastname: Amodaj
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA ; Department of Pathology, University of California, San Francisco, 505 Parnassus Ave, San Francisco, CA 94143 USA.
authors.forename: Henry
authors.initials: H
authors.lastname: Pinkard
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA ; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Ronald D
authors.initials: RD
authors.lastname: Vale
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA ; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.
authors.forename: Nico
authors.initials: N
authors.lastname: Stuurman
keywords: Micro-Manager; high-content screening; high-speed imaging; localization microscopy; open-source microscopy