title: Enhanced camptothecin production by ethanol addition in the suspension culture of the endophyte, Fusarium solani.
abstract: Ethanolic extract of a non-camptothecin producing plant, Catharanthus roseus when added in the suspension culture of the endophyte Fusarium solani known to produce camptothecin, resulted in enhanced production of camptothecin by 10.6-fold in comparison to that in control (2.8 μg/L). Interestingly, addition of pure ethanol (up to 5% v/v) in the suspension culture of F. solani resulted in maximum enhancement in camptothecin production (up to 15.5-fold) from that obtained in control. In the presence of ethanol, a reduced glucose uptake (by ∼ 40%) and simultaneous ethanol consumption (up to 9.43 g/L) was observed during the cultivation period (14 days). Also, the total NAD level and the protein content in the biomass increased by 3.7- and 1.9-fold, respectively, in comparison to that in control. The study indicates a dual role of ethanol, presumably as an elicitor and also as a carbon/energy source, leading to enhanced biomass and camptothecin production.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
authors.forename: Aarthi
authors.initials: A
authors.lastname: Venugopalan
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India. Electronic address: smita@iitm.ac.in.
authors.forename: Smita
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D018533:Biomass; D019149:Bioreactors; D002166:Camptothecin / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D060026:Endophytes / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry*; D005670:Fusarium / Q000378:metabolism*; D005947:Glucose / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D007218:Industrial Microbiology / Q000379:methods; D009243:NAD / Q000737:chemistry
chemical_list: D003470:Culture Media; D009243:NAD; D000431:Ethanol; D002244:Carbon; D005947:Glucose; D005990:Glycerol; D002166:Camptothecin
keywords: Camptothecin; Carbon/energy source; Elicitor; Ethanol; Fusarium solani