title: [Study on tetrodotoxin detection and toxic puffer fish identification of roasted fish fillet at the retail in Beijing and Qingdao].
abstract: OBJECTIVE
  The roasted fish fillet sample at the retail collected in Beijing and Qingdao were detected for TTX, and the TTX positive samples was analyzed for fish species identification.
  
  
  METHODS
  TTX was tested by EUSA method and the cytochrome c oxidase I (COI) genome of TTX-positive samples was extracted and identified by DNA barcode.
  
  
  RESULTS
  Totally, 90 samples were tested by EUSA and 58 (64.4%) samples were positive for TTX with the levels ranging from 0.10 mg/kg to 63.81 mg/kg. Among the TTX positive samples, 24 (41.3%) were identified containing toxic puffer fish and 21 (87.5%) were Lagocephalus lunaris, the highly toxic puffer fish.
  
  
  CONCLUSIONS
  Some roasted fish fillet samples obtained from the retail in two cities were positive for TTX and contained toxic puffer fish. Based on these results, we suggest that roasted fish fillet producers should prevent toxic puffer fish from mixing in the raw material and the I regulators should strengthen the TTX surveillance and product labeling supervision of roasted fish fillet.
authors.affiliation: Medical College, Qingdao University, Qingdao 266021, Shandong Province. shenqing33@126.com
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wang
authors.forename: Chunhui
authors.initials: C
authors.lastname: Han
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Fengqin
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D003132:Commerce; D004247:DNA; D005396:Fish Products / Q000506:poisoning; D011042:Poisons / Q000032:analysis* / Q000633:toxicity; D016133:Polymerase Chain Reaction; D023721:Tetraodontiformes / Q000145:classification / Q000378:metabolism*; D013779:Tetrodotoxin / Q000235:genetics / Q000633:toxicity*; D014020:Tissue Extracts / Q000032:analysis* / Q000633:toxicity
chemical_list: D011042:Poisons; D014020:Tissue Extracts; D013779:Tetrodotoxin; D004247:DNA