title: Establishment and ultrasound characteristics of atherosclerosis in rhesus monkey.
abstract: BACKGROUND
  Atherosclerosis is one of the main risk factors cause acute cerebral-cardio vascular diseases. It's of great significance to establish an atherosclerosis animal model that can mimic the characteristics and nature course of human patients. Therefore, a rhesus monkey model was induced by high-fat diet to monitor their lipid profile and intima-media thickness (IMT) of artery walls and study atherosclerosis progression.
  
  
  METHODS
  Fifty male rhesus monkeys were enrolled in this study. All of these monkeys were aged 7 to 14 years with BMI >30 kg/m2. They were fed with high-fat diet containing 10% of fat for the first 48 weeks. Use ultrasound to measure the IMT at bilateral common carotid arteries and their bifurcations and aorta (AO) of the monkeys, and screen out the individuals with thickened IMT for the next phase. In the next 48 weeks, some of these monkeys (n = 4) were fed with standard diet containing 3% fat. Meanwhile the other monkeys (n = 5) were fed with high-fat diet for another 48 weeks. Their serum lipid level was monitored and arterial IMT was also determined periodically.
  
  
  RESULTS
  Serum lipid level of all 50 monkeys elevated after fed with high-fat diet for the first 48 weeks. IMT thickening at right common carotid bifurcation and aorta (AO) was thickened in 9 monkeys. Furthermore, 4 of these 9 monkeys were fed with standard diet and other 5 monkeys were fed with high-fat diet in the following 48 weeks. The serum lipid level of the 4 monkeys recovered and their IMT at RBIF and AO did not progress. However, the lipid level of other 5 monkeys remained high, and their IMT thickening of AO progressed, and plaques and calcification focuses were found at the anterior wall of aorta near the bifurcation of common iliac artery.
  
  
  CONCLUSIONS
  After high-fat diet induction for 96 weeks, serum lipid levels of rhesus monkeys elevated significantly, which subsequently caused IMT thickening and plaques formation. When IMT thickening occurred, further vascular injury may be prevented by reducing diet fat content. Our study indicates that vascular injury of high-fat diet induced rhesus monkey is similar to that of human in position and progression.
authors.forename: Wen
authors.initials: W
authors.lastname: Zeng
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Wen
authors.forename: Li
authors.initials: L
authors.lastname: Gong
authors.forename: Jiayu
authors.initials: J
authors.lastname: Sun
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Jichun
authors.initials: J
authors.lastname: Liao
authors.forename: Can
authors.initials: C
authors.lastname: Qian
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Song
authors.forename: Fabao
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000000981:diagnostic imaging* / Q000209:etiology; D059168:Carotid Intima-Media Thickness; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D018450:Disease Progression; D008055:Lipids / Q000097:blood; D008253:Macaca mulatta; D008297:Male
chemical_list: D008055:Lipids