title: Photocoagulation of human retinal pigment epithelium in vitro: unravelling the effects on ARPE-19 by transcriptomics and proteomics.
abstract: OBJECTIVE
  Despite the extensive use of retinal photocoagulation for ischaemia and vascular leakage in retinal vascular disease, the molecular mechanisms behind its clinical beneficial effects are still poorly understood. One important target of laser irradiation is the retinal pigment epithelium (RPE). In this study, we aimed at identifying the isolated effects of photocoagulation of RPE at both the mRNA and protein expression levels.
  
  
  METHODS
  Human ARPE-19 cells were exposed to photocoagulation. Gene expression and protein expression were compared to untreated cells using microarray and liquid chromatography-mass spectrometry analysis. Genes and proteins queried by microarray and mass spectrometry were subjected to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database pathway analyses.
  
  
  RESULTS
  Laser irradiation resulted in an induction of the cytoprotective heat-shock protein subfamily Hsp70 as well as in a suppression of the vascular permeability factor carbonic anhydrase 9 (CA9). These expression patterns were evident at both the mRNA and protein levels. KEGG pathway analyses revealed genes and proteins involved in cellular turnover, repair and inflammation.
  
  
  CONCLUSIONS
  By characterizing the transcriptional and translational effects of laser coagulation on the RPE cells in culture, we have revealed responses, which might contribute to some of the beneficial effects obtained by photocoagulation for ischaemia and vascular leakage in retinal vascular disease.
authors.affiliation: Unit on Vascular Diabetic Complications, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Poya
authors.initials: P
authors.lastname: Tababat-Khani
authors.affiliation: Proteomics Laboratory, Vall d'Hebron Institute of Oncology (VHIO), Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Carolina
authors.initials: C
authors.lastname: de la Torre
authors.affiliation: Proteomics Laboratory, Vall d'Hebron Institute of Oncology (VHIO), Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Canals
authors.affiliation: Unit on Diabetes and Celiac Disease, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Hedvig
authors.initials: H
authors.lastname: Bennet
authors.affiliation: Diabetes and Metabolism Research Unit and CIBERDEM, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Simo
authors.affiliation: Diabetes and Metabolism Research Unit and CIBERDEM, Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Hernandez
authors.affiliation: Unit on Diabetes and Celiac Disease, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Fex
authors.affiliation: Unit on Vascular Diabetic Complications, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Carl-David
authors.initials: CD
authors.lastname: Agardh
authors.affiliation: Unit on Diabetes & Endocrinology, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Ola
authors.initials: O
authors.lastname: Hansson
authors.affiliation: Unit on Vascular Diabetic Complications, Department of Clinical Sciences, Lund University Diabetes Center, Malmö, Sweden.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Agardh
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D005136:Eye Proteins / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D017075:Laser Coagulation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D040901:Proteomics; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D055213:Retinal Pigment Epithelium / Q000378:metabolism / Q000601:surgery*; D053719:Tandem Mass Spectrometry; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D005136:Eye Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: microarray; photocoagulation; proteomics; retinal pigment epithelium