title: Evaluation of Mycobacterium bovis double knockout mce2-phoP as candidate vaccine against bovine tuberculosis.
abstract: In this study, a Mycobacterium bovis knockout strain in phoP-phoR and mce2 operons was tested as an antituberculosis experimental vaccine in animal models. The double mutant strain was significantly more attenuated than the wild type strain in inmunocompetent and inmunodeficient mice. Vaccination with the double mutant protected mice against challenge with a virulent M. bovis strain.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: García
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Bianco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: María José
authors.initials: MJ
authors.lastname: Gravisaco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Rosana V
authors.initials: RV
authors.lastname: Rocha
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina.
authors.forename: Federico C
authors.initials: FC
authors.lastname: Blanco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina. Electronic address: bigi.fabiana@inta.gob.ar.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002417:Cattle; D015169:Colony Count, Microbial; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D055786:Gene Knockout Techniques; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009163:Mycobacterium bovis / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity; D018417:Th1 Cells / Q000276:immunology; D032581:Tuberculosis Vaccines / Q000235:genetics* / Q000276:immunology / Q000633:toxicity; D014380:Tuberculosis, Bovine / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000633:toxicity; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D032581:Tuberculosis Vaccines; D014613:Vaccines, Attenuated; C443482:mce2 protein, Mycobacterium tuberculosis; C061064:PhoP protein, Bacteria
keywords: Bovine tuberculosis; Mycobacerium bovis; Vaccine; phoP