title: Elongator, a conserved complex required for wobble uridine modifications in eukaryotes.
abstract: Elongator is a 6 subunit protein complex highly conserved in eukaryotes. The role of this complex has been controversial as the pleiotropic phenotypes of Elongator mutants have implicated the complex in several cellular processes. However, in yeast there is convincing evidence that the primary and probably only role of this complex is in formation of the 5-methoxycarbonylmethyl (mcm(5)) and 5-carbamoylmethyl (ncm(5)) side chains on uridines at wobble position in tRNA. In this review we summarize the cellular processes that have been linked to the Elongator complex and discuss its role in tRNA modification and regulation of translation. We also describe additional gene products essential for formation of ncm(5) and mcm(5) side chains at U34 and their influence on Elongator activity.
authors.affiliation: a Department of Molecular Biology ; Umeå University; Umeå , Sweden.
authors.forename: Tony
authors.initials: T
authors.lastname: Karlsborn
authors.forename: Hasan
authors.initials: H
authors.lastname: Tükenmez
authors.forename: A K M Firoj
authors.initials: AK
authors.lastname: Mahmud
authors.forename: Fu
authors.initials: F
authors.lastname: Xu
authors.forename: Hao
authors.initials: H
authors.lastname: Xu
authors.forename: Anders S
authors.initials: AS
authors.lastname: Byström
mesh_terms: D000818:Animals; D000926:Anticodon / Q000737:chemistry / Q000378:metabolism; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism; D003062:Codon / Q000737:chemistry / Q000378:metabolism; D005815:Genetic Code; D051548:Histone Acetyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D051379:Mice; D012323:RNA Processing, Post-Transcriptional; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D014529:Uridine / Q000235:genetics / Q000378:metabolism*
chemical_list: D000926:Anticodon; D003062:Codon; D007527:Isoenzymes; D016601:RNA-Binding Proteins; D012343:RNA, Transfer; D051548:Histone Acetyltransferases; D014529:Uridine
keywords: KTI genes; Kluveromyces lactis γ-toxin; SAP genes; SIT4; elongator complex; tRNA wobble uridine modifications,; translation