title: Abiotic, biotic, and evolutionary control of the distribution of C and N isotopes in food webs.
abstract: Ecosystem functioning depends on nutrient cycles and their responses to abiotic and biotic determinants, with the influence of evolutionary legacies being generally overlooked in ecosystem ecology. Along a broad elevation gradient characterized by shifting climatic and grazing environments, we addressed clines of plant N and C∶N content and of δ(13)C and δ(15)N in producers (herbs) and in primary (grasshoppers) and secondary (birds) consumers, both within and between species in phylogenetically controlled scenarios. We found parallel and significant intra- and interspecific trends of isotopic variation with elevation in the three groups. In primary producers, nutrient and isotope distributions had a detectable phylogenetic signal that constrained their variation along the environmental gradient. The influence of the environment could not be ascribed to any single factor, and both grazing and climate had an effect on leaf stoichiometry and, thus, on the resources available to consumers. Trends in consumers matched those in plants but often became nonsignificant after controlling for isotopic values of their direct resources, revealing direct bottom-up control and little phylogenetic dependence. By integrating ecosystem and mechanistic perspectives, we found that nutrient dynamics in food webs are governed at the base by the complex interaction between local determinants and evolutionary factors.
authors.affiliation: Research Unit of Biodiversity (Universidad de Oviedo [UO], Consejo Superior de Investigaciones Científicas [CSIC], Principado de Asturias [PA]), Oviedo University, 33600 Mieres, Spain.
authors.forename: Paola
authors.initials: P
authors.lastname: Laiolo
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Illera
authors.forename: Leandro
authors.initials: L
authors.lastname: Meléndez
authors.forename: Amalia
authors.initials: A
authors.lastname: Segura
authors.forename: José Ramón
authors.initials: JR
authors.lastname: Obeso
mesh_terms: D000818:Animals; D005075:Biological Evolution; D001717:Birds; D057486:Carbon Cycle; D002247:Carbon Isotopes / Q000032:analysis; D002980:Climate; D020387:Food Chain; D006110:Grasshoppers / Q000737:chemistry; D060434:Herbivory; D058458:Nitrogen Cycle; D009587:Nitrogen Isotopes / Q000032:analysis; D010944:Plants / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes