title: Hsp90, Hsp60 and HSF-1 genes expression in muscle, heart and brain of thermally manipulated broiler chicken.
abstract: The effect of thermal manipulation (TM) during embryogenesis (ED 12-18) on mRNA expressions of heat shock proteins (Hsp90, Hsp60 and HSF-1) in muscle, heart and brain tissues during thermal challenge (TC) at post-hatching days 10 and 28 was investigated. Fertile chicken eggs were randomly divided into four groups: Control group (37.8 °C), TM1 (39 °C for 9 h), TM2 (39 °C for 12 h) and TM3 (39 °C for 18 h). At days 10 and 28 of age, chicks in TC groups were subjected to thermal challenge (TC) at 43.0 °C for 6 h while naïve chicks were kept under regular conditions. When compared with the control, TM resulted in a significant increase in mRNA levels of Hsp90, Hsp60 and HSF-1in muscle, heart and brain tissues during embryogenesis and during TC at days 10 and 28 post-hatching. These results indicate a long-term enhancement of Hsp90, Hsp60 and HSF-1 gene expressions associated with improved thermotolerance acquisition in thermally manipulated chicks.
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia. Electronic address: alzghoul@just.edu.jo.
authors.forename: Mohammad-Borhan
authors.initials: MB
authors.lastname: Al-Zghoul
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Zuhair Bani
authors.initials: ZB
authors.lastname: Ismail
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abd Elhafeed S
authors.initials: AE
authors.lastname: Dalab
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abdulla
authors.initials: A
authors.lastname: Al-Ramadan
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Thnaian A
authors.initials: TA
authors.lastname: Althnaian
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Saeed Y
authors.initials: SY
authors.lastname: Al-Ramadan
authors.affiliation: Department of Anatomy, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abdelhadi M
authors.initials: AM
authors.lastname: Ali
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Ibrahim F
authors.initials: IF
authors.lastname: Albokhadaim
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Khalid Ahmed
authors.initials: KA
authors.lastname: Al Busadah
authors.affiliation: Department of Physiology and Pharmacology, Faculty of Veterinary Medicine and Animal Resources, King Faisal University, Al-Hasa, Saudi Arabia.
authors.forename: Abdulhakeem
authors.initials: A
authors.lastname: Eljarah
authors.affiliation: Department of Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, Jordan.
authors.forename: Khaleel I
authors.initials: KI
authors.lastname: Jawasreh
authors.affiliation: Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, United States.
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Hannon
mesh_terms: D000818:Animals; D001831:Body Temperature / Q000502:physiology; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D018834:Chaperonin 60 / Q000235:genetics / Q000378:metabolism*; D002642:Chick Embryo / Q000254:growth & development / Q000378:metabolism; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006321:Heart / Q000196:embryology; D000076249:Heat Shock Transcription Factors; D006358:Hot Temperature; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013963:Thyroid Hormones / Q000097:blood; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D004268:DNA-Binding Proteins; D018841:HSP90 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; D012333:RNA, Messenger; D013963:Thyroid Hormones; D014157:Transcription Factors
keywords: Heat shock factor-1 (HSF-1); Heat shock protein 60 (Hsp60); Heat shock protein 90 (Hsp90); Thermal manipulation; Thermotolerance