title: InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis Meyen in burn injuries.
abstract: Therapies that accelerate the healing of burn injuries, improving the quality of life of the patient and reducing the cost of treatment are important. This study evaluated the effects of InGaP 670-nm laser therapy combined with a hydroalcoholic extract of Solidago chilensis leaves on burn wound healing in rats. Seventy-two rats were divided randomly into four groups: control untreated (C), treated with InGaP 670-nm laser with power density of 0.41 W/cm(2) and energy density of 4.93 J/cm(2) (L), treated with S. chilensis extract (S) and treated with S. chilensis extract and laser (LS). Second-degree burns were produced on the back of the animals with metal plate. Wound samples were collected on days 7, 14 and 21 of treatment for structural analysis, morphometry and Western blotting to quantify the expression of transforming growth factor beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF). The results showed that InGaP laser irradiation at 670 nm alone and combined with extract of S. chilensis promoted significant tissue repair responses in this experimental model, increasing the number of fibroblasts, collagen fibres and newly formed blood vessels throughout the experimental period and decreasing the number of granulocytes in burn wounds of second degree in all treated groups. Exclusive treatment of burn wounds with the hydroalcoholic extract of S. chilensis provided similar quantitative results to those seen in the untreated group throughout the experimental period. Therefore, it was observed in the L and LS groups different responses in the expression of TGF-β1 and VEGF. The application of 670-nm laser alone or combined with the extract of S. chilensis promoted favourable responses in tissue repair of second-degree burns in this experimental model.
authors.affiliation: Graduate Program in Biomedical Sciences, Herminio Ometto University Center (UNIARARAS), Avenida Maximiliano Baruto, 500, Jardim Universitário, CEP 13607-339, Araras, SP, Brazil, helenreinhart@uol.com.br.
authors.forename: Helen Reinhart Camargo
authors.initials: HR
authors.lastname: Catarino
authors.forename: Natália Pereira
authors.initials: NP
authors.lastname: de Godoy
authors.forename: Nayara Kastem
authors.initials: NK
authors.lastname: Scharlack
authors.forename: Lia Mara Grosso
authors.initials: LM
authors.lastname: Neves
authors.forename: Fernanda Oliveira de Gaspari
authors.initials: FO
authors.lastname: de Gaspi
authors.forename: Marcelo Augusto Marreto
authors.initials: MA
authors.lastname: Esquisatto
authors.forename: Maria Esméria Corezola
authors.initials: ME
authors.lastname: do Amaral
authors.forename: Fernanda Aparecida Sampaio
authors.initials: FA
authors.lastname: Mendonça
authors.forename: Gláucia Maria Tech
authors.initials: GM
authors.lastname: dos Santos
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism / Q000628:therapy*; D003131:Combined Modality Therapy; D005347:Fibroblasts / Q000378:metabolism; D028022:Low-Level Light Therapy; D008297:Male; D018919:Neovascularization, Physiologic / Q000528:radiation effects; D000073718:Pancreatitis-Associated Proteins; D010936:Plant Extracts / Q000627:therapeutic use*; D017208:Rats, Wistar; D012867:Skin / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects; D036624:Solidago / Q000737:chemistry*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D000073718:Pancreatitis-Associated Proteins; D010936:Plant Extracts; C000612141:REG3A protein, human; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A