title: In vivo and ex vivo magnetic resonance spectroscopy of the infarct and the subventricular zone in experimental stroke.
abstract: Ex vivo high-resolution magic-angle spinning (HRMAS) provides metabolic information with higher sensitivity and spectral resolution than in vivo magnetic resonance spectroscopy (MRS). Therefore, we used both techniques to better characterize the metabolic pattern of the infarct and the neural progenitor cells (NPCs) in the ipsilateral subventricular zone (SVZi). Ischemic stroke rats were divided into three groups: G0 (non-stroke controls, n = 6), G1 (day 1 after stroke, n = 6), and G7 (days 6 to 8 after stroke, n = 12). All the rats underwent MRS. Three rats per group were analyzed by HRMAS. The remaining rats were used for immunohistochemical studies. In the infarct, both techniques detected significant metabolic changes. The most relevant change was in mobile lipids (2.80 ppm) in the G7 group (a 5.53- and a 3.95-fold increase by MRS and HRMAS, respectively). In the SVZi, MRS did not detect any significant metabolic change. However, HRMAS detected a 2.70-fold increase in lactate and a 0.68-fold decrease in N-acetylaspartate in the G1 group. None of the metabolites correlated with the 1.37-fold increase in NPCs detected by immunohistochemistry in the G7 group. In conclusion, HRMAS improves the metabolic characterization of the brain in experimental ischemic stroke. However, none of the metabolites qualifies as a surrogate biomarker of NPCs.
authors.affiliation: Departament de Neurologia, Institut d'Investigació Biomèdica de Sant Pau (IIB), Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Jiménez-Xarrié
authors.affiliation: 1] Departament de Bioquímica i Biologia Molecular, Unitat de Biociències, Edifici C, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [2] Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [3] Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Cerdanyola del Vallès, Spain.
authors.forename: Myriam
authors.initials: M
authors.lastname: Davila
authors.affiliation: 1] Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, CIBERNED, Valencia, Spain [2] Unidad Mixta de Neurorregeneración, Fundación para la Investigación La Fe, Valencia, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Gil-Perotín
authors.affiliation: Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, CIBERNED, Valencia, Spain.
authors.forename: Andrés
authors.initials: A
authors.lastname: Jurado-Rodríguez
authors.affiliation: 1] Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Cerdanyola del Vallès, Spain [2] Departament de Bioquímica i Biologia Molecular, Unitat de Biociències, Edifici C, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [3] Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Candiota
authors.affiliation: Departament de Neurologia, Institut d'Investigació Biomèdica de Sant Pau (IIB), Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Delgado-Mederos
authors.affiliation: 1] Servei de RMN, Edifici C, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [2] Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Cerdanyola del Vallès, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Lope-Piedrafita
authors.affiliation: 1] Laboratorio de Neurobiología Comparada, Instituto Cavanilles, Universidad de Valencia, CIBERNED, Valencia, Spain [2] Unidad Mixta de Neurorregeneración, Fundación para la Investigación La Fe, Valencia, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: García-Verdugo
authors.affiliation: 1] Departament de Bioquímica i Biologia Molecular, Unitat de Biociències, Edifici C, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [2] Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain [3] Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Cerdanyola del Vallès, Spain.
authors.forename: Carles
authors.initials: C
authors.lastname: Arús
authors.affiliation: Departament de Neurologia, Institut d'Investigació Biomèdica de Sant Pau (IIB), Hospital de la Santa Creu i Sant Pau, Barcelona, Spain.
authors.forename: Joan
authors.initials: J
authors.lastname: Martí-Fàbregas
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D020520:Brain Infarction / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D020547:Lateral Ventricles / Q000378:metabolism* / Q000473:pathology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: D015415:Biomarkers