title: Regulated genes in mesenchymal stem cells and gastric cancer.
abstract: OBJECTIVE
  To investigate the genes regulated in mesenchymal stem cells (MSCs) and diffuse-type gastric cancer (GC), gene expression was analyzed.
  
  
  METHODS
  Gene expression of MSCs and diffuse-type GC cells were analyzed by microarray. Genes related to stem cells, cancer and the epithelial-mesenchymal transition (EMT) were extracted from human gene lists using Gene Ontology and reference information. Gene panels were generated, and messenger RNA gene expression in MSCs and diffuse-type GC cells was analyzed. Cluster analysis was performed using the NCSS software.
  
  
  RESULTS
  The gene expression of regulator of G-protein signaling 1 (RGS1) was up-regulated in diffuse-type GC cells compared with MSCs. A panel of stem-cell related genes and genes involved in cancer or the EMT were examined. Stem-cell related genes, such as growth arrest-specific 6, musashi RNA-binding protein 2 and hairy and enhancer of split 1 (Drosophila), NOTCH family genes and Notch ligands, such as delta-like 1 (Drosophila) and Jagged 2, were regulated.
  
  
  CONCLUSIONS
  Expression of RGS1 is up-regulated, and genes related to stem cells and NOTCH signaling are altered in diffuse-type GC compared with MSCs.
authors.affiliation: Shihori Tanabe, Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Shihori
authors.initials: S
authors.lastname: Tanabe
authors.affiliation: Shihori Tanabe, Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Aoyagi
authors.affiliation: Shihori Tanabe, Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yokozaki
authors.affiliation: Shihori Tanabe, Division of Safety Information on Drug, Food and Chemicals, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Sasaki
keywords: Epithelial-mesenchymal transition; Gastric cancer; Gene; Mesenchymal stem cells; Stem cells