title: Identification of plant compounds that disrupt the insect juvenile hormone receptor complex.
abstract: Insects impact human health through vector-borne diseases and cause major economic losses by damaging crops and stored agricultural products. Insect-specific growth regulators represent attractive control agents because of their safety to the environment and humans. We identified plant compounds that serve as juvenile hormone antagonists (PJHANs). Using the yeast two-hybrid system transformed with the mosquito JH receptor as a reporter system, we demonstrate that PJHANs affect the JH receptor, methoprene-tolerant (Met), by disrupting its complex with CYCLE or FISC, formation of which is required for mediating JH action. We isolated five diterpene secondary metabolites with JH antagonist activity from two plants: Lindera erythrocarpa and Solidago serotina. They are effective in causing mortality of mosquito larvae at relatively low LD50 values. Topical application of two diterpenes caused reduction in the expression of Met target genes and retardation of follicle development in mosquito ovaries. Hence, the newly discovered PJHANs may lead to development of a new class of safe and effective pesticides.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea;
authors.forename: Seok-Hee
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Industrial Bio-Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea; hwoh@kribb.re.kr alexander.raikhel@ucr.edu btrus@snu.ac.kr shiwoongg@gmail.com.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Oh
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea;
authors.forename: Ying
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea;
authors.forename: Saes-Byeol
authors.initials: SB
authors.lastname: An
authors.affiliation: Microbiological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea;
authors.forename: Doo-Sang
authors.initials: DS
authors.lastname: Park
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 363-883, Republic of Korea;
authors.forename: Hyuk-Hwan
authors.initials: HH
authors.lastname: Song
authors.affiliation: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang 363-883, Republic of Korea;
authors.forename: Sei-Ryang
authors.initials: SR
authors.lastname: Oh
authors.affiliation: National Institute of Biological Resources, Incheon 404-708, Republic of Korea;
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Applied Entomology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-100, Republic of Korea;
authors.forename: Seonghyun
authors.initials: S
authors.lastname: Kim
authors.affiliation: Applied Entomology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-100, Republic of Korea;
authors.forename: Namjung
authors.initials: N
authors.lastname: Kim
authors.affiliation: Department of Entomology, University of California, Riverside, CA 92521; and hwoh@kribb.re.kr alexander.raikhel@ucr.edu btrus@snu.ac.kr shiwoongg@gmail.com.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Raikhel
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea hwoh@kribb.re.kr alexander.raikhel@ucr.edu btrus@snu.ac.kr shiwoongg@gmail.com.
authors.forename: Yeon Ho
authors.initials: YH
authors.lastname: Je
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Republic of Korea; hwoh@kribb.re.kr alexander.raikhel@ucr.edu btrus@snu.ac.kr shiwoongg@gmail.com.
authors.forename: Sang Woon
authors.initials: SW
authors.lastname: Shin
mesh_terms: D000818:Animals; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D060434:Herbivory / Q000187:drug effects*; D019476:Insect Proteins / Q000378:metabolism*; D007313:Insecta / Q000187:drug effects* / Q000254:growth & development; D007605:Juvenile Hormones / Q000037:antagonists & inhibitors*; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D027443:Lindera / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036624:Solidago / Q000737:chemistry*; D020798:Two-Hybrid System Techniques
chemical_list: D004224:Diterpenes; D019476:Insect Proteins; D007605:Juvenile Hormones
keywords: endocrine disruptor; juvenile hormone; receptor