title: Thirst driving and suppressing signals encoded by distinct neural populations in the brain.
abstract: Thirst is the basic instinct to drink water. Previously, it was shown that neurons in several circumventricular organs of the hypothalamus are activated by thirst-inducing conditions. Here we identify two distinct, genetically separable neural populations in the subfornical organ that trigger or suppress thirst. We show that optogenetic activation of subfornical organ excitatory neurons, marked by the expression of the transcription factor ETV-1, evokes intense drinking behaviour, and does so even in fully water-satiated animals. The light-induced response is highly specific for water, immediate and strictly locked to the laser stimulus. In contrast, activation of a second population of subfornical organ neurons, marked by expression of the vesicular GABA transporter VGAT, drastically suppresses drinking, even in water-craving thirsty animals. These results reveal an innate brain circuit that can turn an animal's water-drinking behaviour on and off, and probably functions as a centre for thirst control in the mammalian brain.
authors.affiliation: 1] Department of Biochemistry and Molecular Biophysics, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA [2] Department of Neuroscience, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Oka
authors.affiliation: 1] Department of Biochemistry and Molecular Biophysics, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA [2] Department of Neuroscience, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Ye
authors.affiliation: 1] Department of Biochemistry and Molecular Biophysics, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA [2] Department of Neuroscience, Columbia College of Physicians and Surgeons, Howard Hughes Medical Institute, Columbia University, New York, New York 10032, USA.
authors.forename: Charles S
authors.initials: CS
authors.lastname: Zuker
mesh_terms: D000818:Animals; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D003681:Dehydration / Q000503:physiopathology; D004326:Drinking; D004327:Drinking Behavior / Q000502:physiology*; D060766:Drinking Water; D007834:Lasers; D051379:Mice; D062308:Optogenetics; D012528:Satiety Response; D013356:Subfornical Organ / Q000166:cytology* / Q000502:physiology*; D013894:Thirst / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D060766:Drinking Water; C490641:Etv1 protein, mouse; D014157:Transcription Factors; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2