title: Droplet electrochemical study of the pH dependent redox behavior of novel ferrocenyl-carborane derivatives and its application in specific cancer cell recognition.
abstract: Novel ferrocenyl based carboranes (FcCBs) and their distinguish behavior for cancer cell recognition have been explored in this contribution. The voltammetric study in a droplet of 10μL placed on the surface of a glassy carbon electrode demonstrates the excellent electrochemical behavior of FcCBs, which could be further exploited for establishing the promising and sensitive biosensors. The FcCBs' redox behavior is examined in a wide pH range, and square wave voltammetry revealed the reversible and irreversible nature of first and second anodic peaks. The obvious shifts in peak potentials corresponding with the change of pH values demonstrate the abstraction of electrons to be accompanied with the transfer of protons. By using the droplet electrochemical technique, FcCBs can be employed to distinguish normal and cancer cells with a linear range from 1.0×10(3) to 3.0×10(4)cells mL(-1) and the limit of detection at 800cells mL(-1). The novel carborane derivatives could be utilized as important potential molecular probes for specific recognition of cancer cells like leukemia cells from normal cells.
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, PR China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Afzal
authors.initials: A
authors.lastname: Shah
authors.affiliation: State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China.
authors.forename: Hongde
authors.initials: H
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Hematology, the Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing 210009, PR China.
authors.forename: Baoan
authors.initials: B
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing 210096, PR China. Electronic address: xuewang@seu.edu.cn.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, PR China. Electronic address: hyan1965@nju.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry / Q000633:toxicity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D020014:K562 Cells / Q000187:drug effects; D007938:Leukemia / Q000175:diagnosis*; D007962:Leukocytes / Q000737:chemistry / Q000473:pathology; D057230:Limit of Detection; D015336:Molecular Probe Techniques; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011522:Protons; D012016:Reference Values; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005296:Ferrous Compounds; D015335:Molecular Probes; D011522:Protons
keywords: Cancer cell recognition; Droplet electrochemistry; Ferrocenyl based carboranes; Leukemia