title: The g.841G>C SNP of FASN gene is associated with fatty acid composition in beef cattle.
abstract: The objective of the current study is to evaluate the association between fatty acid composition and fatty acid synthase gene polymorphisms as responsible mutations. For this purpose, we selected seven previously reported single nucleotide polymorphisms (SNPs) in FASN gene, including one within promoter region (g.841G>C) and six non-synonymous SNPs (g.8805C>T, g.13126C>T, g.15532A>C, g.16024A>G, g.16039C>T, g.17924A>G), and genotyped them in Japanese Black cattle. Genotyping results revealed that g.8805 C>T and g.17924 A>G were monomorphic loci. Genome-wide association analysis including the other five SNPs revealed that only g.841G>C showed significant associations with the percentages of C14:0, C14:1, C16:1 and C18:1 at 5% genome-wide significance level. In order to further evaluate the effect, we genotyped g.841G>C using additional three populations, including two Japanese Black populations and a Holstein cattle population. g.16024A>G was also genotyped and included in the analysis because it has been reported to be associated with fatty acid composition in Japanese Black cattle. In the result of analysis of variance, g.841G>C showed stronger effects on fatty acid percentage than those of g.16024A>G in all populations. These results suggested that g.841G>C would be a responsible mutation for fatty acid composition and contribute to production of high-grade beef as a selection marker in beef cattle.
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Kiri
authors.initials: K
authors.lastname: Hayakawa
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Sakamoto
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishii
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Keita
authors.initials: K
authors.lastname: Yamaji
authors.affiliation: National Livestock Breeding Center, Nishigo, Fukushima, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Uemoto
authors.affiliation: National Livestock Breeding Center, Nishigo, Fukushima, Japan.
authors.forename: Nanae
authors.initials: N
authors.lastname: Sasago
authors.affiliation: National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan.
authors.forename: Naohiko
authors.initials: N
authors.lastname: Kobayashi
authors.affiliation: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan.
authors.forename: Tamako
authors.initials: T
authors.lastname: Matsuhashi
authors.affiliation: Gifu Prefectural Livestock Research Institute, Takayama, Gifu, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Maruyama
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Matsumoto
authors.affiliation: Food Resources Education & Research Center, Kobe University, Kasai, Hyogo, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Oyama
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Mannen
authors.affiliation: Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Sasazaki
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002417:Cattle / Q000235:genetics*; D054890:Fatty Acid Synthase, Type I / Q000235:genetics*; D005227:Fatty Acids / Q000032:analysis*; D063427:Food Quality; D005819:Genetic Markers; D055106:Genome-Wide Association Study; D060005:Genotyping Techniques; D008460:Meat / Q000032:analysis; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D005227:Fatty Acids; D005819:Genetic Markers; D054890:Fatty Acid Synthase, Type I
keywords: FASN; GWAS; Holstein; Japanese Black cattle; fatty acid composition