title: A molecular pathway for CO₂ response in Arabidopsis guard cells.
abstract: Increasing carbon dioxide (CO₂) levels in the atmosphere have caused global metabolic changes in diverse plant species. CO₂ is not only a carbon donor for photosynthesis but also an environmental signal that regulates stomatal movements and thereby controls plant-water relationships and carbon metabolism. However, the mechanism underlying CO₂ sensing in stomatal guard cells remains unclear. Here we report characterization of Arabidopsis RESISTANT TO HIGH CO₂ (RHC1), a MATE-type transporter that links elevated CO₂ concentration to repression of HT1, a protein kinase that negatively regulates CO₂-induced stomatal closing. We also show that HT1 phosphorylates and inactivates OST1, a kinase which is essential for the activation of the SLAC1 anion channel and stomatal closing. Combining genetic, biochemical and electrophysiological evidence, we reconstituted the molecular relay from CO₂ to SLAC1 activation, thus establishing a core pathway for CO₂ signalling in plant guard cells.
authors.affiliation: 1] College of Life Sciences, Capital Normal University, Beijing 100048, China [2] Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: Wang
authors.initials: W
authors.lastname: Tian
authors.affiliation: 1] College of Life Sciences, Capital Normal University, Beijing 100048, China [2] Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: Congcong
authors.initials: C
authors.lastname: Hou
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Ren
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Jia
authors.affiliation: 1] College of Life Sciences, Capital Normal University, Beijing 100048, China [2] Plant Molecular Biology Institute and College of Life Sciences, Nanjing University, Nanjing 210097, China.
authors.forename: Haiwen
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Fenglin
authors.initials: F
authors.lastname: Bai
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: He
authors.affiliation: State key laboratory of chemo/Biosensing and chemometries, Hunan University, Changsha 410082, China.
authors.forename: Shenglian
authors.initials: S
authors.lastname: Luo
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing 100048, China.
authors.forename: Legong
authors.initials: L
authors.lastname: Li
authors.affiliation: 1] Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA [2] Plant Molecular Biology Institute and College of Life Sciences, Nanjing University, Nanjing 210097, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism; D010788:Photosynthesis / Q000502:physiology; D059828:Plant Cells / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D026901:Membrane Transport Proteins; D002245:Carbon Dioxide