title: High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96.
abstract: Vitamin D deficiency or insufficiency is an independent risk factor for diabetic peripheral neuropathy (DPN), but the relationship between 1,25(OH)2D3 and DPN remains unknown. We found that 1,25(OH)2D3 stimulated the secretion of nerve growth factor (NGF) in rat Schwann cell line RSC96, but ability of 1,25(OH)2D3 to increase NGF protein was impaired under high glucose conditions. High glucose upregulated the expression of CYP24A1 protein, which catalyzes the conversion of 1,25(OH)2D3 into inactive products, further impairing the ability of 1,25(OH)2D3 to upregulate NGF secretion in Schwann cells. Inhibition of CYP24A1 protein expression ameliorated the secretion of NGF in response to 1,25(OH)2D3. The findings of this study suggest that CYP24A1 protein plays an important role in the relationship between DPN and 1,25(OH)2D3.
authors.affiliation: Department of Endocrinology and Metabolism, First People's Hospital of Yunnan Province (The Kunhua Affiliated Hospital of Kunming University of Science and Technology), Kunming, Yunnan, China.
authors.forename: Yi-Kun
authors.initials: YK
authors.lastname: Zhou
authors.affiliation: Department of Information Center, First People's Hospital of Yunnan Province (The Kunhua Affiliated Hospital of Kunming University of Science and Technology), Kunming, Yunnan, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Liang
authors.affiliation: The State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Chongqing Center for Biomedical Research and Equipment Development, Chongqing Academy of Science and Technology, Chongqing, China. Electronic address: zht79571@163.com.
authors.forename: Hua-Tang
authors.initials: HT
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, First People's Hospital of Yunnan Province (The Kunhua Affiliated Hospital of Kunming University of Science and Technology), Kunming, Yunnan, China. Electronic address: hua64482@sina.com.
authors.forename: Kun-Hua
authors.initials: KH
authors.lastname: Wang
mesh_terms: D000818:Animals; D002117:Calcitriol / Q000493:pharmacokinetics* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003929:Diabetic Neuropathies / Q000201:enzymology; D019833:Genistein / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D015854:Up-Regulation; D065668:Vitamin D3 24-Hydroxylase / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D020932:Nerve Growth Factor; D019833:Genistein; C585551:Cyp24a1 protein, rat; D065668:Vitamin D3 24-Hydroxylase; D002117:Calcitriol; D005947:Glucose
keywords: 1,25(OH)(2)D(3); CYP24A1; High glucose