title: Simultaneous determination of monosaccharides and oligosaccharides in dates using liquid chromatography-electrospray ionization mass spectrometry.
abstract: Ultra performance liquid chromatography coupled to mass spectrometry was used for the simultaneous separation and determination of reducing monosaccharides (fructose and glucose), a non-reducing disaccharide (sucrose) and oligosaccharides (kestose and nystose) in HILIC mode. The chromatographic separation of all saccharides was performed on a BEH amide column using an acetonitrile-water gradient elution. The detection was carried out using selected ion recording (SIR) acquisition mode. The validation of the proposed method showed that the limit of detection and limit of quantification values for the five analyzed compounds were in the range of 0.25-0.69μg/mL and 0.82-3.58μg/mL, respectively; while the response was linear in the range of 1-50μg/mL. The developed method showed potential usefulness for a rapid and sensitive analysis of underivatized saccharides and was used for determination of sugars in three date samples (Sefri, Mabroom, Ghassab) which were soxhlet extracted by ethanol.
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia.
authors.forename: Ayman A
authors.initials: AA
authors.lastname: Ghfar
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia. Electronic address: swabaidur@ksu.edu.sa.
authors.forename: Saikh M
authors.initials: SM
authors.lastname: Wabaidur
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia.
authors.forename: A Yacine Badjah Hadj
authors.initials: AY
authors.lastname: Ahmed
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Alothman
authors.affiliation: Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, 11451 Riyadh, Saudi Arabia.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Khan
authors.affiliation: Department of Chemistry, College of Science, Princess Nora Bint Abdulrahman University, P.O. Box 84428, 11671 Riyadh, Saudi Arabia.
authors.forename: Nora H
authors.initials: NH
authors.lastname: Al-Shaalan
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D009005:Monosaccharides / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides
keywords: Liquid chromatography; Mass spectrometry; Monosaccharides; Oligosaccharides; Palm dates