title: The effects of fluoxetine on circulating oxidative damage parameters in rats exposed to aortic ischemia-reperfusion.
abstract: Oxidative stress and reperfusion injury may develop in different ischemia-reperfusion (IR) models. Growing evidence links altered lipid protein redox-homeostasis with IR. The effect of fluoxetine (FLX; N-methyl-3-[4-(trifluoromethyl) phenoxy] benzenepropanamine), on the lipid protein redox-homeostasis mechanisms in the rats exposed to aortic IR is unclear. We aimed to investigate the effects of FLX on circulating protein oxidation and lipid peroxidation parameters, such as ischemia modified albumin (IMA), lipid hydroperoxide (LOOH), prooxidant antioxidant balance (PAB), erythrocyte glutathione (GSH), CuZn-superoxide dismutase (CuZn-SOD), ferric reducing antioxidant power (FRAP), as potential IR biomarkers. Wistar rats were randomized into three groups (n=7/group): 1) Control (sham laparotomy); 2) IR without FLX, (60min ischemia and 120min reperfusion); 3) IR with FLX (FLX+IR) (FLX 20mg/kg/day, i.p. for three days before surgery). All of the aforementioned parameters (IMA, LOOH, PAB, GSH, CuZn-SOD, and FRAP) were measured spectrophotometrically. IMA, LOOH, and PAB levels in IR group were significantly higher than the control (P<0.01 respectively) and fluoxetine groups (P<0.01, P<0.01, and P<0.05 respectively), whereas CuZn-SOD activities, GSH and FRAP were significantly lower in IR groups. Fluoxetine group significantly reduced IMA when compared to IR group (P<0.001) and control group (P<0.01). With respect to IMA, LOOH and PAB, impaired redox homeostasis is substantially more prominent in aortic IR. The antidepressant FLX has profitable effects on circulating redox status in rats exposed to aortic IR. FLX administration before IR might decrease the surgery-enhanced free radical production; taken together, the antioxidant effects of FLX supplementation should be considered in future studies.
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Hayriye
authors.initials: H
authors.lastname: Erman
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Physiology, Istanbul, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Guner
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Physiology, Istanbul, Turkey.
authors.forename: Muhittin Onur
authors.initials: MO
authors.lastname: Yaman
authors.affiliation: Istanbul University, Istanbul Medical Faculty, Istanbul, Turkey.
authors.forename: Duygu Derya
authors.initials: DD
authors.lastname: Uzun
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Remise
authors.initials: R
authors.lastname: Gelisgen
authors.affiliation: Science Faculty, Department of Biology, Istanbul University, Istanbul, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Aksu
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Physiology, Istanbul, Turkey.
authors.forename: Nermin
authors.initials: N
authors.lastname: Yelmen
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Gulderen
authors.initials: G
authors.lastname: Sahin
authors.affiliation: Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey. Electronic address: huzun59@hotmail.com.
authors.forename: Hafize
authors.initials: H
authors.lastname: Uzun
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001012:Aorta, Abdominal / Q000378:metabolism / Q000601:surgery*; D001219:Aspartate Aminotransferases / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D005473:Fluoxetine / Q000494:pharmacology*; D005978:Glutathione / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D012709:Serum Albumin / Q000032:analysis
chemical_list: D000975:Antioxidants; D008054:Lipid Peroxides; D012709:Serum Albumin; D005473:Fluoxetine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D005978:Glutathione
keywords: Aortic ischemia–reperfusion; Ferric reducing antioxidant power; Fluoxetine; Ischemia modified albumin; Lipid hydroperoxide; Pro-oxidant–antioxidant balance