title: The Expression and Significance of the Plasma Let-7 Family in Anti-N-methyl-D-aspartate Receptor Encephalitis.
abstract: The study aimed to investigate the expression and significance of the plasma let-7 family in anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis. Blood samples from 5 anti-NMDAR encephalitis patients and 5 negative controls were collected for microarray analysis. Blood samples from10 anti-NMDAR encephalitis patients, 10 anti-NMDAR encephalitis patients whose physical conditions have improved after 3 months of immunotherapy, 20 virus (meningitis) encephalitis patients, 20 tuberculosis (meningitis) encephalitis patients, 10 purulent (meningitis) encephalitis patients, 20 cerebral cysticercosis patients, 20 ischemic stroke patients, 20 intracerebral hemorrhage patients, 15 neuromyelitis optica patients, 15 multiple sclerosis patients, 15 moyamoya disease patients, and 20 negative controls were collected for real-time quantitative PCR (qRT-PCR) analysis. The expression levels of let-7a, let-7b, let-7d, and let-7f were significantly down-regulated in anti-NMDAR encephalitis compared with the negative controls (NC). The expression levels of let-7a, let-7d, and let-7f were significantly down-regulated in other nervous system diseases compared with the NC group while the expression level of let-7b was statistically insignificant in other nervous system diseases compared with the NC group. In addition, there was no significant dysregulation of let-7b in the anti-NMDAR encephalitis treatment group compared with the NC. Let-7b may be a potential diagnostic marker and an indicator that reflected the molecular mechanism of anti-NMDAR encephalitis.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, 1st Jian She East Road, Zhengzhou, People's Republic of China, 450052, zj1513645@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Xu
authors.forename: Shaoyun
authors.initials: S
authors.lastname: Zhao
authors.forename: Zhe
authors.initials: Z
authors.lastname: Gong
authors.forename: Peidong
authors.initials: P
authors.lastname: Liu
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Guan
authors.forename: Xia
authors.initials: X
authors.lastname: He
authors.forename: Tianshu
authors.initials: T
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Peng
authors.forename: Junfang
authors.initials: J
authors.lastname: Teng
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000328:Adult; D060426:Anti-N-Methyl-D-Aspartate Receptor Encephalitis / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C487931:mirnlet7 microRNA, human