title: Magnetic-activated cell sorting (MACS) can be used as a large-scale method for establishing zebrafish neuronal cell cultures.
abstract: Neuronal cell cultures offer a crucial tool to mechanistically analyse regeneration in the nervous system. Despite the increasing importance of zebrafish (Danio rerio) as an in vivo model in neurobiological and biomedical research, in vitro approaches to the nervous system are lagging far behind and no method is currently available for establishing enriched neuronal cell cultures. Here we show that magnetic-activated cell sorting (MACS) can be used for the large-scale generation of neuronal-restricted progenitor (NRP) cultures from embryonic zebrafish. Our findings provide a simple and semi-automated method that is likely to boost the use of neuronal cell cultures as a tool for the mechanistic dissection of key processes in neuronal regeneration and development.
authors.affiliation: 1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
authors.forename: Georg
authors.initials: G
authors.lastname: Welzel
authors.affiliation: 1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
authors.forename: Daniel
authors.initials: D
authors.lastname: Seitz
authors.affiliation: 1] Department of Animal Physiology, University of Bayreuth, 95440 Bayreuth, Germany [2] Friedrich-Baur BioMed Center, 95448 Bayreuth.
authors.forename: Stefan
authors.initials: S
authors.lastname: Schuster
mesh_terms: D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002469:Cell Separation; D002478:Cells, Cultured; D004625:Embryo, Nonmammalian / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D009415:Nerve Net / Q000187:drug effects / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000378:metabolism
chemical_list: D029961:Zebrafish Proteins; D014212:Tretinoin