title: In vitro expansion of tumour cells derived from blood and tumour tissue is useful to redefine personalized treatment in non-small cell lung cancer patients.
abstract: The clinical development of locally and advanced non-small cell lung cancer (NSCLC) suffers from a lack of biomarkers as a guide in the selection of optimal prognostic prediction. Circulating Tumour Cells (CTCs) are correlated to prognosis and show efficacy in cancer monitoring in patients. However, their enumeration alone might be inadequate; it might also be critical to understand the viability, the apoptotic state and the kinetics of these cells. Here, we report what we believe to be a new and selective approach to visually detect tumour specific CTCs. Firstly, using labelled human lung cancer cells, we detected a specific density interval in which NSCL-CTCs were concentrated. Secondly, to better characterize CTCs in respect to their heterogeneous composition and tumour reference, blood and tumour biopsy were performed on specimens taken from the same patient. The approach consisted in comparing phenotype profile of CTCs, and their progenitor Tumour Stem Cells, (TSCs). Moreover, NSCL-CTCs were cultivated in short-time human cultures to provide response to drug sensitivity. Our bimodal approach allowed to reveal two items. Firstly, that one part of a tumour, proximal to the bronchial structure, displays a predominance of CD133+. Secondly, specific NSCL-CTCs Epithelial Cell Adhesion Molecule (EpCAM)+CD29+ can be used as a negative prognostic factor as well the high expression of CTCs EpCAM+. These data were confirmed by drug-sensitivity tests, in vitro, and by the survival curves, in vivo.
authors.affiliation: Italian Institutes of Technology, Arnesano, Lecce, Italy.
authors.forename: N M
authors.initials: NM
authors.lastname: Malara
authors.affiliation: Thoracic Surgery, Cancer Centre of Excellence, University Magna Graecia of Catanzaro, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Givigliano
authors.affiliation: Cellular Toxicological Laboratory, Department of Health Science, Salvatore Venuta Campus, University Magna Graecia Catanzaro, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Trunzo
authors.affiliation: University of Medicine San Raffaele, Milano, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Macrina
authors.affiliation: Systems Biology, University College of Dublin, Belfied, Republic of Ireland.
authors.forename: C
authors.initials: C
authors.lastname: Raso
authors.affiliation: Department of Experimental and Clinical Medicine, Salvatore Venuta Campus, University Magna Graecia of Catanzaro, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Amodio
authors.affiliation: Association ExChanger: Share your Science,Catanzaro, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Aprigliano
authors.affiliation: Association ExChanger: Share your Science,Catanzaro, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Minniti
authors.affiliation: Cellular Toxicological Laboratory, Department of Health Science, Salvatore Venuta Campus, University Magna Graecia Catanzaro, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.affiliation: Oncology Surgery, Cancer Centre of Excellence, University Magna Graecia of Catanzaro, (Italy).
authors.forename: L
authors.initials: L
authors.lastname: Roveda
authors.affiliation: Bionem Laboratories, Department of Experimental and Clinical Medicine, Salvatore Venuta Campus, University Magna Graecia of Catanzaro, Italy.
authors.forename: M L
authors.initials: ML
authors.lastname: Coluccio
authors.affiliation: IRCCS San Raffaele Pisana, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.affiliation: Thoracic Surgery, Cancer Centre of Excellence, University Magna Graecia of Catanzaro, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Voci
authors.affiliation: Oncology Surgery, Cancer Centre of Excellence, University Magna Graecia of Catanzaro, (Italy).
authors.forename: U
authors.initials: U
authors.lastname: Prati
authors.affiliation: Physical Science and Engineering Division, King Abdullah University of Science and Technology, Kingdom of Saudi Arabia.
authors.forename: E
authors.initials: E
authors.lastname: Di Fabrizio
authors.affiliation: Interregional Research Center for Food Safety and Health (IRC-FSH), Catanzaro, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Mollace
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology* / Q000628:therapy; D002453:Cell Cycle; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology* / Q000628:therapy; D016246:Lymphocytes, Tumor-Infiltrating / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009360:Neoplastic Cells, Circulating; D057285:Precision Medicine