title: Essential role of TNF-alpha in development of spleen fibroblastic reticular cells.
abstract: TNF-alpha plays an important role in the development of secondary lymphoid tissues. Earlier studies showed that fibroblastic reticular cells express TNF-alpha receptor, suggesting that TNF-alpha may affect the development of FRCs. To test this, we analyzed the development and function of FRCs in wild-type or TNF-alpha knockout mice. We found that GP38 expression was down-regulated in the spleen of TNF-alpha knockout mice. Chemokines, mainly secreted by GP38(+) FRCs, were also down-regulated. Additionally, we found that absence of TNF-alpha decreased the homing ability to direct T cells to the spleen. However, absence of TNF-alpha did not affect the development of lymph nodes FRCs. These data reveal that TNF-alpha plays an important role in the development of spleen FRCs. Absence of TNF-alpha could cause abnormality of spleen FRCs, thereby weakening the homing ability of T cells to localize to the spleen T cell zone.
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: chaoren72@126.com.
authors.forename: Lintao
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: yangoncology@163.com.
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: lln372@126.com.
authors.forename: Lina
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: nwgjb@163.com.
authors.forename: Jianbao
authors.initials: J
authors.lastname: Gao
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China; Department of Immunology, Third Military Medical University, Chongqing, China. Electronic address: guobomail@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Guo
authors.affiliation: Institution of Oncology, Xinqiao Hospital, Third Military Medical University, Chongqing, China. Electronic address: b.davis.zhu@gmail.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D018925:Chemokines / Q000235:genetics / Q000276:immunology; D015536:Down-Regulation / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D012313:RNA / Q000737:chemistry / Q000235:genetics; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*; D000098853:Podoplanin
chemical_list: D018925:Chemokines; D008562:Membrane Glycoproteins; D012313:RNA; D014409:Tumor Necrosis Factor-alpha; C490441:Pdpn protein, mouse; D000098853:Podoplanin
keywords: Fibroblastic reticular cells; GP38; Spleen; TNF-alpha