title: [Significance of Septin9 gene methylation detection of plasma circulation DNA in colorectal cancer screening].
abstract: OBJECTIVE
  To explore the role of detecting the methylation status of gene Septin9 (SEPT9) in plasma for colorectal cancer screening in Chinese population.
  
  
  METHODS
  Patients were collected from Beijing Military General Hospital since September 2013 to February 2014. The performance of SEPT9 assay was validated in a single-blind study of 80 cases with colonoscopy and pathologically verified colorectal cancer and 52 normal controls. The detection of Septin9 gene methylation in peripheral blood was performed by fluorescence quantitative polymerase chain reaction (PCR). And immunoassay fecal occult blood test was conducted to compare the superiority of methylated Septin9 for screening colorectal cancer.
  
  
  RESULTS
  The Septin9 assay successfully identified 75.0% (95%CI:64.7%-83.6%) of cancers at a specificity of 98.1% (95%CI:90.9%-99.9%). And it was superior to fecal occult blood screening for colorectal cancer (sensitivity 79.5% vs 53.8%, P < 0.05).
  
  
  CONCLUSIONS
  Determination of SEPT9 methylation status is an innovative non-invasive plasma screening test for colorectal cancer.
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Kang
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Hejuan
authors.initials: H
authors.lastname: An
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China. Email: jianqiu@263.net.
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Sheng
mesh_terms: D044466:Asian People; D003113:Colonoscopy; D015179:Colorectal Neoplasms; D004247:DNA; D019175:DNA Methylation; D055088:Early Detection of Cancer; D006801:Humans; D058112:Septins; D016037:Single-Blind Method
chemical_list: D004247:DNA; C474315:SEPTIN9 protein, human; D058112:Septins