title: Egg yolks inhibit activation of NF-κB and expression of its target genes in adipocytes after partial delipidation.
abstract: How composition of egg yolk (EY) influences NF-κB, a key transcription pathway in inflammation, remains unclear. We performed partial delipidation of EY that removed 20-30% of cholesterol and triglycerides. The resulting polar and nonpolar fractions were termed EY-P and EY-NP. NF-κB activation in response to EY from different suppliers and their fractions was examined in 3T3-L1 adipocytes using a NF-κB response element reporter assay and by analyzing expression of 248 inflammatory genes. Although EY-P and EY contained similar level of vitamins, carotenoids, and fatty acids, only delipidated EY-P fraction suppressed NF-κB via down-regulation of toll like receptor-2 and up-regulation of inhibitory toll interacting protein (Tollip) and lymphocyte antigen 96 (Ly96). Our data suggest that anti-inflammatory activity of lutein and retinol were blunted by nonpolar lipids in EY, likely via crosstalk between SREBP and NF-κB pathways in adipocytes. Thus, moderate delipidation may improve the beneficial properties of regular eggs.
authors.affiliation: Department of Human Sciences, The Ohio State University , 1787 Neil Avenue, 331A Campbell Hall, Columbus, Ohio 43210, United States.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Shen
authors.forename: Ken M
authors.initials: KM
authors.lastname: Riedl
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Cole
authors.forename: Christopher
authors.initials: C
authors.lastname: Lehman
authors.forename: Lu
authors.initials: L
authors.lastname: Xu
authors.forename: Hansjuerg
authors.initials: H
authors.lastname: Alder
authors.forename: Martha A
authors.initials: MA
authors.lastname: Belury
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Schwartz
authors.forename: Ouliana
authors.initials: O
authors.lastname: Ziouzenkova
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D002645:Chickens; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004530:Egg Yolk / Q000737:chemistry* / Q000378:metabolism; D005511:Food Handling; D005786:Gene Expression Regulation; D007249:Inflammation / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D008055:Lipids / Q000737:chemistry*; D014975:Lutein / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D014801:Vitamin A / Q000378:metabolism
chemical_list: D016207:Cytokines; D008055:Lipids; D016328:NF-kappa B; D014801:Vitamin A; D014975:Lutein
keywords: NF-κB; delipidation; egg yolk; inflammation; lipids; vitamins