title: Multifunctional assembly of micrometer-sized colloids for cell sorting.
abstract: Compared to the extensively studied nanometer-sized colloids, less attention has been paid to the assembly of micrometer-sized colloids with multifunctional characteristics. To address this need, a bottom-up approach is developed for constructing self-assemblies of micrometer-sized magnetic colloids possessing multifunctionality, including magnetic, optical, and biological activities. Biotinylated oligo (p-phenylene vinylene) (OPV) derivatives are designed to mediate the self-assembly of streptavidin-modified magnetic beads. The optical element OPV derivatives provide a fluorescence imaging ability for tracing the assembly process. Target cells can be recognized and assembled by the colloidal assembly with bioactive element antibodies. The colloidal assembly reveals better cell isolation performance by its amplified magnetic response in comparison to monodisperse colloids. The self-assembly of micrometer-sized magnetic colloids through a combination of different functional ingredients to realize multifunction is conceptually simple and easy to achieve.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Chenyao
authors.initials: C
authors.lastname: Nie
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Jiangyan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D019921:Biotinylation; D002469:Cell Separation / Q000379:methods*; D003102:Colloids; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D061986:MCF-7 Cells; D008280:Magnetics; D018613:Microscopy, Confocal; D010316:Particle Size; D011145:Polyvinyls / Q000737:chemistry; D019809:Streptavidin
chemical_list: D003102:Colloids; D011145:Polyvinyls; C530822:oligo(p-phenylene vinylene); D019809:Streptavidin
keywords: cell sorting; colloids; oligo (p-phenylene vinylene); self-assembly