title: Lack of association between MIF gene -173G>C polymorphism with multiple sclerosis.
abstract: OBJECTIVE
  The aim of the present study was to investigate a possible association between the MIF -173G>C polymorphism and MS in Turkish patients.
  
  
  METHODS
  The study included 153 MS-patients and 210 controls. Genomic DNA was isolated and genotyped using PCR-RFLP assay for the MIF -173G>C promoter polymorphism (rs755622).
  
  
  RESULTS
  There was no statistically significant difference in allele and genotype frequencies between MS-patients and controls (p=0.227 and p=0.157, respectively). Accordingly, no association was observed when the patients were compared against controls in terms of GG versus GC+CC genotypes and GG+GC versus CC genotypes (p=0.324 and p=0.179, respectively). Also, there was no statistically significant association between MIF-173G>C polymorphism and clinical and demographic characteristics of MS-patients. Conlusion: The results of the present study suggest no relation between MS susceptibility and MIF gene - 173G>C polymorphism in the examined Turkish population.
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Neurology, Tokat, Turkey cevikbetul78@hotmail.com.
authors.forename: Betul
authors.initials: B
authors.lastname: Cevik
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Serbulent
authors.initials: S
authors.lastname: Yigit
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Nevin
authors.initials: N
authors.lastname: Karakus
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Neurology, Tokat, Turkey.
authors.forename: Durdane
authors.initials: D
authors.lastname: Aksoy
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Medical Biology, Tokat, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Ates
authors.affiliation: Gaziosmanpasa University Faculty of Medicine, Department of Neurology, Tokat, Turkey.
authors.forename: Semiha
authors.initials: S
authors.lastname: Kurt
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors
chemical_list: D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C515598:MIF protein, human
keywords: -173G>C gene polymorphism; Multiple sclerosis; macrophage migration inhibitory factor (MIF); promoter polymorphism; susceptibility