title: Long noncoding RNA GAS5: a novel marker involved in glucocorticoid response.
abstract: Glucocorticoids (GCs) exert their effects through regulation of gene expression after activation in the cytoplasm of the glucocorticoid receptor (GR) encoded by NR3C1 gene. A negative feedback mechanism resulting in GR autoregulation has been demonstrated through the binding of the activated receptor to intragenic sequences called GRE-like elements, contained in GR gene. The long noncoding RNA growth arrest-specific transcript 5 (GAS5) interacts with the activated GR suppressing its transcriptional activity. The aim of this study was to evaluate the possible role of GAS5 and NR3C1 gene expression in the antiproliferative effect of methylprednisolone in peripheral blood mononuclear cells and to correlate the expression with individual sensitivity to GCs. Subjects being poor responders to GCs presented higher levels of GAS5 and NR3C1 in comparison with good responders. We suggest that abnormal levels of GAS5 may alter GC effectiveness, probably interfering with the mechanism of GR autoregulation.
authors.forename: M
authors.initials: M
authors.lastname: Lucafo
authors.forename: S
authors.initials: S
authors.lastname: De Iudicibus
authors.forename: A
authors.initials: A
authors.lastname: Di Silvestre
authors.forename: M
authors.initials: M
authors.lastname: Pelin
authors.forename: L
authors.initials: L
authors.lastname: Candussio
authors.forename: S
authors.initials: S
authors.lastname: Martelossi
authors.forename: A
authors.initials: A
authors.lastname: Tommasini
authors.forename: E
authors.initials: E
authors.lastname: Piscianz
authors.forename: A
authors.initials: A
authors.lastname: Ventura
authors.affiliation: University of Trieste, Via Fleming 22, Trieste 34127, Italy. decorti@units.it.
authors.forename: G
authors.initials: G
authors.lastname: Decorti
mesh_terms: D000328:Adult; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects; D008297:Male; D008775:Methylprednisolone / Q000008:administration & dosage; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
chemical_list: C561567:GAS5 long non-coding RNA, human; D005938:Glucocorticoids; C497713:NR3C1 protein, human; D062085:RNA, Long Noncoding; D011965:Receptors, Glucocorticoid; D008775:Methylprednisolone