title: Differential modulation of macrophage response elicited by glycoinositolphospholipids and lipophosphoglycan from Leishmania (Viannia) shawi.
abstract: In this work, some aspects of the glycobiology of Leishmania shawi were examined, as it is a causative agent of cutaneous leishmaniasis in the New World. Additionally, the interaction of L. shawi's main glycoconjugates [lipophosphoglycan (LPG) and glycoinositolphospholipids (GIPLs)] with macrophages was evaluated in vitro. L. shawi LPG was devoid of side-chains in its repeat units, whereas monosaccharide analysis showed that GIPLs were suggestive of mannose-rich (type I or hybrid). In order to evaluate the biological roles of those molecules, BALB/c resident peritoneal macrophages were incubated with these glycoconjugates for 24h, and the levels of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-12p70 and IL-10, were determined. In general, the GIPLs exhibited a greater proinflammatory role than the LPGs did. However, for the first time, the GIPLs from this species were able to trigger the production of IL-10, an anti-inflammatory cytokine. In conclusion, L. shawi glycoconjugates were able to interact with the innate immune compartment. These data reinforce the role of parasite glycoconjugates during parasite and host cell interactions.
authors.affiliation: Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: felipepassero@yahoo.com.br.
authors.forename: Luiz F D
authors.initials: LF
authors.lastname: Passero
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Rafael R
authors.initials: RR
authors.lastname: Assis
authors.affiliation: Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Thays N F
authors.initials: TN
authors.lastname: da Silva
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Nogueira
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Diego H
authors.initials: DH
authors.lastname: Macedo
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Natália L
authors.initials: NL
authors.lastname: Pessoa
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Marco A
authors.initials: MA
authors.lastname: Campos
authors.affiliation: Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Márcia D
authors.initials: MD
authors.lastname: Laurenti
authors.affiliation: Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima, 1715, 30190-002 Belo Horizonte, MG, Brazil.
authors.forename: Rodrigo P
authors.initials: RP
authors.lastname: Soares
mesh_terms: D000818:Animals; D006001:Glycoconjugates / Q000276:immunology*; D006028:Glycosphingolipids / Q000737:chemistry / Q000276:immunology*; D006790:Host-Parasite Interactions; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D007891:Leishmania / Q000737:chemistry* / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D010716:Phosphatidylinositols / Q000737:chemistry / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D006001:Glycoconjugates; D006028:Glycosphingolipids; C556062:IL10 protein, mouse; D010716:Phosphatidylinositols; D014409:Tumor Necrosis Factor-alpha; C008290:lipophosphonoglycan; D016753:Interleukin-10; D018664:Interleukin-12; D009569:Nitric Oxide
keywords: Glycoinositolphospholipids; Interaction; Leishmania (Viannia) shawi; Lipophosphoglycan