title: Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment.
abstract: The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), production of reactive oxygen species (ROS), and total antioxidant status (TAS) in porcine ovarian granulosa cells after quercetin and T-2 toxin exposure in vitro. Porcine ovarian granulosa cells were incubated with quercetin and T-2 toxin separately or in mutual combination at the doses of 1 ng/mL, 10 ng/mL, 100 ng/mL, and the control group without any additions for 24 h. In this study T-2 toxin developed stress reaction in porcine ovarian granulosa cells and increased generation of ROS. Quercetin had no effect in elimination of ROS generation induced by T-2 toxin, but was effective in maintaining and increasing of TAS, activities of SOD and GPx in porcine granulosa cells in vitro. These results contribute towards the understanding of cellular stress and its response.
authors.affiliation: a Slovak University of Agriculture in Nitra , Faculty of Biotechnology and Food Sciences, Department of Animal Physiology , Nitra, Slovak Republic.
authors.forename: Marcela
authors.initials: M
authors.lastname: Capcarova
authors.forename: Peter
authors.initials: P
authors.lastname: Petruska
authors.forename: Katarina
authors.initials: K
authors.lastname: Zbynovska
authors.forename: Adriana
authors.initials: A
authors.lastname: Kolesarova
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Sirotkin
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D010053:Ovary / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects; D011794:Quercetin / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine; D013605:T-2 Toxin / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D011794:Quercetin; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D013605:T-2 Toxin
keywords: Porcine granulosa cells; T-2 toxin; antioxidants; quercetin; reactive oxygen species