title: CDy6, a photostable probe for long-term real-time visualization of mitosis and proliferating cells.
abstract: Long-term real-time visualization of lysosomal dynamics has been challenging at the onset of mitosis due to the lack of fluorescent probes enabling convenient imaging of dividing cells. We developed a long-term real-time photostable mitotic or proliferating marker, CDy6, a BODIPY-derived compound of designation yellow 6, which labels lysosome. In long-term real-time, CDy6 displayed a sharp increase in intensity and change in localization in mitosis, improved photostability, and decreased toxicity compared with other widely used lysosomal and DNA markers, and the ability to label cells in mouse xenograft models. Therefore, CDy6 may open new possibilities to target and trace lysosomal contents during mitosis and to monitor cell proliferation, which can further our knowledge of the basic underlying biological mechanisms in the management of cancer.
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore.
authors.forename: Yun-Mi
authors.initials: YM
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Zhai
authors.initials: Z
authors.lastname: Duanting
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
authors.forename: Holger
authors.initials: H
authors.lastname: Hennig
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore.
authors.forename: Animesh
authors.initials: A
authors.lastname: Samanta
authors.affiliation: Department of Chemistry, National University of Singapore, Singapore 117543, Singapore.
authors.forename: Bikram Keshari
authors.initials: BK
authors.lastname: Agrawalla
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
authors.forename: Mark-Anthony
authors.initials: MA
authors.lastname: Bray
authors.affiliation: Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
authors.forename: Anne E
authors.initials: AE
authors.lastname: Carpenter
authors.affiliation: Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency of Science, Technology and Research, Singapore 138667, Singapore; Department of Chemistry, National University of Singapore, Singapore 117543, Singapore. Electronic address: chmcyt@nus.edu.sg.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D008938:Mitosis; D059008:Time-Lapse Imaging; D014183:Transplantation, Heterologous
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; C000602706:yellow 6