title: Androctonus australis hector venom contributes to the interaction between neuropeptides and mast cells in pulmonary hyperresponsiveness.
abstract: Lung injury and respiratory distress syndrome are frequent symptoms observed in the most severe cases of scorpion envenomation. The uncontrolled transmigration of leukocyte cells into the lung interstitium and alveolar space and pulmonary edema may be the cause of death. Mast cells can release various inflammatory mediators known to be involved in the development of lung edema following scorpion venom injection. The present study was designed to determine the evidence of neurokinin 1 (NK1) receptor and the involvement of mast cell activation to induce pulmonary edema and to increase vascular permeability after Androctonus australis hector (Aah) venom administration. To this end, mast cells were depleted using compound 48/80 (C48/80). Furthermore, the involvement of tachykinin NK1 receptors expressed on mast cell membranes was elucidated by their blocking with an antagonist. On the other hand, the ability of Aah venom to increase vascular permeability and to induce edema was also assessed by measuring the amount of Evans blue dye (EBD) extravasation in bronchoalveolar lavage (BAL) fluid and in the lungs of mice. Pulmonary edema, as assessed by the levels of EBD extravasation, was completely inhibited in compound 48/80-treated animals. Depletion by stimuli non-immunological C48/80 component markedly reduced induced inflammatory response following the venom administration. The mast cells seem to play an important role in the development of lung injury and the increase of vascular permeability in mice following the subcutaneous administration of Aah scorpion venom through the NK1 receptor.
authors.affiliation: University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Algiers, Algeria.
authors.forename: Imène
authors.initials: I
authors.lastname: Chaïr-Yousfi
authors.affiliation: University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Algiers, Algeria. Electronic address: flaraba@hotmail.com.
authors.forename: Fatima
authors.initials: F
authors.lastname: Laraba-Djebari
authors.affiliation: University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, Laboratory of Cellular and Molecular Biology, Algiers, Algeria.
authors.forename: Djelila
authors.initials: D
authors.lastname: Hammoudi-Triki
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000276:immunology*; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D006801:Humans; D007211:Indoles / Q000008:administration & dosage; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D064729:Neurokinin-1 Receptor Antagonists / Q000008:administration & dosage; D010880:Piperidines / Q000008:administration & dosage; D011654:Pulmonary Edema / Q000139:chemically induced / Q000276:immunology*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D065008:Scorpion Stings / Q000276:immunology*; D012604:Scorpion Venoms / Q000008:administration & dosage* / Q000009:adverse effects; D012605:Scorpions / Q000276:immunology; D015320:Tachykinins / Q000378:metabolism
chemical_list: C096759:1-(N-(2-methoxybenzyl)acetylamino)-3-(1H-indol-3-yl)-2-(N-(2-(4-(piperidin-1-yl)piperidin-1-yl)acetyl)amino)propane; D007211:Indoles; D064729:Neurokinin-1 Receptor Antagonists; D010880:Piperidines; D018040:Receptors, Neurokinin-1; D012604:Scorpion Venoms; D015320:Tachykinins
keywords: Androctonus australis hector venom; IgE antibodies; Lung edema; Mast cells; NK1 receptor antagonist; Substance P