title: Quantitative histochemistry of the primate skin. III. Glyceraldehyde-3-phosphate dehydrogenase.
abstract: Glyceraldehyde-3-phosphate dehydrogenase activities were assayed with a fluorometric micromethod in various parts of the skin and its appendages. Enzyme activity was abundant, particularly in the epidermis and mucous membrane (4.2 to 11.3 moles/hr/kg dry wt.). The keratin layer of sole epidermis also contained a considerable amount of enzyme activity (2.6 moles/hr/kg dry wt.). A relatively low activity was found in the apocrine and sebaceous glands (1.8 to 3.6 moles/hr/kg dry wt.). The enzyme was influenced by various activators and inhibitors. The activators were 1-cysteine (200% increase), mercaptoethanol (+300%), EDTA (+300%), and the inhibitors, pCMB, Cu++ and Co++ (nearly 100% inhibition). Either arsenate or orthophosphate was an absolute requirement for the enzyme reaction. It is speculated that this dehydrogenase in skin and appendages may be a regulator of the Pasteur effect, hence of glycolysis, as it may be in muscle or in ascites tumor cells.
authors.forename: M J
authors.initials: MJ
authors.lastname: Im
authors.forename: S
authors.initials: S
authors.lastname: Yamasawa
authors.forename: K
authors.initials: K
authors.lastname: Adachi
mesh_terms: D000818:Animals; D001050:Apocrine Glands / Q000201:enzymology; D020536:Enzyme Activators / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005470:Fluorometry; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis / Q000502:physiology*; D008251:Macaca; D008253:Macaca mulatta; D009092:Mucous Membrane / Q000201:enzymology*; D012627:Sebaceous Glands / Q000201:enzymology; D012867:Skin / Q000201:enzymology*
chemical_list: D020536:Enzyme Activators; D004791:Enzyme Inhibitors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases