title: Transcription-coupled recruitment of human CHD1 and CHD2 influences chromatin accessibility and histone H3 and H3.3 occupancy at active chromatin regions.
abstract: BACKGROUND
  CHD1 and CHD2 chromatin remodeling enzymes play important roles in development, cancer and differentiation. At a molecular level, the mechanisms are not fully understood but include transcriptional regulation, nucleosome organization and turnover.
  
  
  RESULTS
  Here we show human CHD1 and CHD2 enzymes co-occupy active chromatin regions associated with transcription start sites (TSS), enhancer like regions and active tRNA genes. We demonstrate that their recruitment is transcription-coupled. CHD1 and CHD2 show distinct binding profiles across active TSS regions. Depletion of CHD1 influences chromatin accessibility at TSS and enhancer-like chromatin regions. CHD2 depletion causes increased histone H3 and reduced histone variant H3.3 occupancy.
  
  
  CONCLUSIONS
  We conclude that transcription-coupled recruitment of CHD1 and CHD2 occurs at transcribed gene TSSs and at intragenic and intergenic enhancer-like sites. The recruitment of CHD1 and CHD2 regulates the architecture of active chromatin regions through chromatin accessibility and nucleosome disassembly.
authors.affiliation: Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge, 141 83 Sweden.
authors.forename: Lee
authors.initials: L
authors.lastname: Siggens
authors.affiliation: Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge, 141 83 Sweden.
authors.forename: Lina
authors.initials: L
authors.lastname: Cordeddu
authors.affiliation: Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge, 141 83 Sweden.
authors.forename: Michelle
authors.initials: M
authors.lastname: Rönnerblad
authors.affiliation: Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge, 141 83 Sweden.
authors.forename: Andreas
authors.initials: A
authors.lastname: Lennartsson
authors.affiliation: Department of Biosciences and Nutrition, NOVUM, Karolinska Institutet, Huddinge, 141 83 Sweden.
authors.forename: Karl
authors.initials: K
authors.lastname: Ekwall
keywords: CHD1; CHD2; DNase; ENCODE; H3; H3.3; chromatin remodeling