title: Research of shRNAmir inhibitory effects towards focal adhesion kinase expression in the treatment of gastric cancer.
abstract: Gastric cancer is the fourth most common type of malignant tumor, with a poor prognosis. Focal adhesion kinase (FAK) mediates the crosslink of intracellular signaling networks, playing a key role in cell migration and invasion. The aim of the present study was to investigate the effects of FAK interference on the proliferation ability, invasion and metastasis of gastric cancer cells. The FAK-RNAi lentiviral vector was infected into SGC7901 gastric cancer cells in order to observe thein vivosituations of tumor growth and metastasis before and after the FAK interference. The growth of SGC7901 gastric cancer cells in the interference group was significantly inhibited compared with that of the negative control (P<0.05) and the blank control groups (P<0.05), and the FAK expression significantly decreased (P<0.05). Thein vitroinvasion and metastasis experiments showed that the cell invasion and metastasis abilities of the interference group significantly decreased when compared with those of the negative control (P<0.05) and blank control groups (P<0.05). In the nude mouse subcutaneous tumor transplantation model, the mean ± standard deviation tumor weight of the interference group (1.474±0.9840 g) was lower than that of the negative control (3.134±0.3299 g) and blank control (2.68±0.12 g) groups (P<0.05). In the nude mice, the liver and peritoneal metastasis rates of the interference group were significantly lower than those of the negative control (P<0.05) and the blank control groups (P<0.05), and the FAK mRNA of the interference group significantly reduced (P<0.05). In conclusion, FAK interference could effectively suppress the proliferation, invasion and metastasis of transfected SGC7901 gastric cancer cells, and could inhibit the growth and distant metastasis of gastric cancer in nude mice.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Su
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Fu-Xing
authors.initials: FX
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: He-Hui
authors.initials: HH
authors.lastname: Mao
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Zheng
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: Department of Ultrasound Diagnosis, The First Affiliated Hospital, Xiamen University, Xiamen, Fujian 361003, P.R. China.
authors.forename: Jing-Jun
authors.initials: JJ
authors.lastname: Su
keywords: RNAi; focal adhesion kinase; gastric cancer; invasion; metastasis; nude mouse