title: Determination of acrylamide in Sudanese food by high performance liquid chromatography coupled with LTQ Orbitrap mass spectrometry.
abstract: A sample preparation method based on modified Quick, Easy, Cheap Effective, Rugged and Safe (QuEChERS) with aluminum oxide (Al2O3) as dispersive solid phase extraction (dSPE) material and high performance liquid chromatography-linear trap quadruple-Orbitrap-mass spectrometry (HPLC LTQ-Orbitrap MS) was established. The performance of two analytical columns namely Kinetex C18 and Rezex ROA-organic acid was compared for acrylamide separation. The method was validated in term of matrix effect, linear range (standard addition method), limit of detection (LOD), limit of quantification (LOQ), precision (RSD%) and recovery. Good linearity (r(2)>0.9979) was achieved using standard addition method in the concentration range 0-200μgkg(-1). The LOD is in the range from 2.91 to 4.04μgkg(-1) and 1.50 to 3.94μgkg(-1) for C18 and ROA columns, respectively. The precision of the method was ⩽7.3% and 5.6% for C18 and ROA columns, respectively. Recoveries of acrylamide ranging from 90% to 97%, (n=3) were obtained. The proposed Al2O3 dSPE method was successfully applied to the analysis of acrylamide in real food samples.
authors.affiliation: Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany; Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum 11115, Sudan; Central Laboratory, Ministry of Sciences & Technology, P.O. Box Office 7099, Khartoum, Sudan.
authors.forename: Mei Musa Ali
authors.initials: MM
authors.lastname: Omar
authors.affiliation: Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany; Department of Chemistry, Faculty of Science, University of Khartoum, Khartoum 11115, Sudan. Electronic address: aaelbashir@uofk.edu.
authors.forename: Abdalla Ahmed
authors.initials: AA
authors.lastname: Elbashir
authors.affiliation: Applied Analytical Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany. Electronic address: oliver.schmitz@uni-due.de.
authors.forename: Oliver J
authors.initials: OJ
authors.lastname: Schmitz
mesh_terms: D020106:Acrylamide / Q000032:analysis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D063427:Food Quality; D057230:Limit of Detection; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013397:Sudan
chemical_list: D020106:Acrylamide
keywords: Acrylamide; Dispersive solid phase extraction; HPLC LTQ-Orbitrap MS; QuEChERS