title: Expression profiles of antiviral response genes in chicken bursal cells stimulated with Toll-like receptor ligands.
abstract: Cells of the adaptive immune system express Toll-like receptors (TLRs) and are able to respond to TLR ligands. With this in mind, the goal of the current study was to determine the expression of antiviral response genes in the cells of the chicken bursa of Fabricius (BF) to stimulation with TLR ligands. We investigated initially the response of bursal B cells to CpG-ODN, lipopolysaccharide (LPS) and poly(I:C) treatment. The expression level of type I interferons (IFNs) and interferon regulatory factor 7 (IRF7) did not differ between CpG-ODN and LPS treated groups compared to the non-stimulated cells. Poly(I:C) was the only TLR ligand, which has induced significant expression of antiviral innate immune response genes from bursal cells. Further in vitro and in vivo studies need to examine the efficacy of these antiviral responses against avian viruses.
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Tamiru Negash
authors.initials: TN
authors.lastname: Alkie
authors.affiliation: Immunology and Medical Biophysics, Faculty of Medicine, University of Toronto, Toronto, ON M5G 2M9, Canada.
authors.forename: Michael
authors.initials: M
authors.lastname: St Paul
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Neda
authors.initials: N
authors.lastname: Barjesteh
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Brisbin
authors.affiliation: Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address: shayan@uoguelph.ca.
authors.forename: Shayan
authors.initials: S
authors.lastname: Sharif
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D002060:Bursa of Fabricius / Q000166:cytology*; D002645:Chickens; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D011070:Poly I-C / Q000494:pharmacology*; D051193:Toll-Like Receptors; D059467:Transcriptome
chemical_list: C408982:CPG-oligonucleotide; D008024:Ligands; D008070:Lipopolysaccharides; D009838:Oligodeoxyribonucleotides; D051193:Toll-Like Receptors; D011070:Poly I-C
keywords: Antiviral genes; Bursal cells; Chicken; Toll-like receptor ligands