title: Testicular hypofunction caused by activating p53 expression induced by reactive oxygen species in varicocele rats.
abstract: We investigated the mechanism of the testicular hypofunction induced by hypoxia in varicocele rats. Varicocele was induced by partial ligation of the left renal vein. Seven weeks later, left testis mass index was measured. The sperm counts and motility were tested by CASA. The change of seminiferous tubule tissue was observed by HE staining. The reactive oxygen species (ROS) level in left testicular tissue was measured by ELISA, and the expressions of HIF-1α and p53 were detected by immunohistochemistry and Western blot. The left testis mass index and the sperm motility were significantly lower in surgery group. By HE staining, the left seminiferous epithelial cell arrangement was incompact, disordered and vacuolated in surgery group. The ROS level in surgery group was significantly higher than the other groups. The results of immunohistochemistry and Western blot indicated that the expressions of HIF-1α and p53 increased significantly in surgery group. Our study demonstrated that varicocele caused hypoxia that could cause the rise of ROS level to induce the increase of p53 expression, leading to the decrease of testis mass index and changes of seminiferous tubules, which would reduce sperm motility and result in male infertility eventually.
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, China.|Reproductive Medical Center, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: M
authors.initials: M
authors.lastname: Liang
authors.affiliation: Reproductive Medical Center, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, China.
authors.forename: J
authors.initials: J
authors.lastname: Wen
authors.affiliation: National Health and Family Planning Commission of the People's Republic of China, Beijing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Reproductive Medicine Research Center, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
authors.forename: L-G
authors.initials: LG
authors.lastname: Zhao
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, China.
authors.forename: B-K
authors.initials: BK
authors.lastname: Shi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013076:Sperm Count; D013081:Sperm Motility; D013737:Testis / Q000503:physiopathology*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism / Q000502:physiology*; D014646:Varicocele / Q000503:physiopathology*
chemical_list: D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53
keywords: Hypoxia-inducible factor-1 alpha subunit; reactive oxygen species; testicular hypofunction; tumour suppressor protein p53; varicocele