title: The association of lipids with keratinous proteins of human callus.
abstract: 1. Small amounts of phospholipids and cho- lesterol bound to major callus protein fractions during extraction have been quantitated. 2. Extraction with chloroform/methanol abolishes the requirement of these protein fractions for prolonged acid hydrolysis in order to release completely certain amino acid residues. 3. The binding capacity of lipid-free, isolated, keratinous proteins for cholesterol has been studied. 4. A plausible (though unproven) role for phospholipids, cholesterol, and keratin in the barrier functions of epidermis is re-emphasized.
authors.affiliation: Department of Medicine, The Johns Hopkins University, Baltimore, MD, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Crounse
mesh_terms: D002146:Bony Callus / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007633:Keratins / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry
chemical_list: D008055:Lipids; D010743:Phospholipids; D007633:Keratins; D002784:Cholesterol