title: 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize.
abstract: In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.
authors.affiliation: REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Fátima Barroso
authors.affiliation: REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal; Dpto. Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Freitas
authors.affiliation: REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, Porto 4050-313, Portugal.
authors.forename: M Beatriz P P
authors.initials: MBPP
authors.lastname: Oliveira
authors.affiliation: Dpto. Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain. Electronic address: santosnoemi@uniovi.es.
authors.forename: Noemí
authors.initials: N
authors.lastname: de-Los-Santos-Álvarez
authors.affiliation: Dpto. Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Lobo-Castañón
authors.affiliation: REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Delerue-Matos
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D015342:DNA Probes / Q000138:chemical synthesis / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D030821:Plants, Genetically Modified; D013439:Sulfhydryl Reagents / Q000737:chemistry; D014050:Toluene / Q000031:analogs & derivatives / Q000737:chemistry; D003313:Zea mays / Q000235:genetics*
chemical_list: D015342:DNA Probes; D013439:Sulfhydryl Reagents; D014050:Toluene; D006046:Gold; C004848:dithiol
keywords: GMO; Genosensor; MON810 maize; Nanostructured 3D Au electrodes; Screen-printed electrodes