title: [SNP-19 genotypic variants of CAPN10 gene and its relation to diabetes mellitus type 2 in a population of Ciudad Juarez, Mexico].
abstract: BACKGROUND
  Diabetes Mellitus (DM) type 2 is a common pathology with multifactorial etiology, which exact genetic bases remain unknown. Some studies suggest that single nucleotides polymorphisms (SNPs) in the CAPN10 gene (Locus 2q37.3) could be associated with the development of this disease, including the insertion/deletion polymorphism SNP-19 (2R→3R).
  
  
  OBJECTIVE
  The present study determined the association between the SNP-19 and the risk of developing DM type 2 in Ciudad Juarez population.
  
  
  METHODS
  For this study 107 participants were selected: 43 diabetics type 2 (cases) and 64 non diabetics with no family history of DM type 2 in first grade (control). Anthropometric studies were realized as well as lipids, lipoproteins and serum glucose biochemical profiles. The genotypification of SNP-19 was performed using peripheral blood lymphocytes DNA, polymerase chain reactions (PCR), and electrophoretic analysis in agarose gels. Once obtained the genotypic and allelic frequencies, the Hardy-Weinberg equilibrium test (GenAlEx 6.4) was also performed.
  
  
  RESULTS
  Using the X² analysis it was identified the genotypic differences between cases and control with higher frequency of the homozygous genotype 3R of SNP- 19 in the cases group (0.418) compared to control group (0.265). Also, it was observed an association between genotype 2R/3R with elevated weight, body mass index, and waist and hip circumferences, but only in the diabetic group (P=< 0.05).
  
  
  CONCLUSIONS
  The findings in this study suggest that SNP-19 in CAPN10 may participate in the development of DM type 2 in the studied population.
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Loya Méndez
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: Gilberto
authors.initials: G
authors.lastname: Reyes Leal
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: Adriana
authors.initials: A
authors.lastname: Sánchez González
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: Verónica
authors.initials: V
authors.lastname: Portillo Reyes
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: David
authors.initials: D
authors.lastname: Reyes Ruvalcaba
authors.affiliation: Laboratorio de Genética aplicada del Instituto de Ciencias Biomédicas de la Universidad Autónoma de Ciudad Juárez. México.. yloya@uacj.mx.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Bojórquez Rangel
mesh_terms: D000328:Adult; D000368:Aged; D002154:Calpain / Q000235:genetics*; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D002154:Calpain; C415197:calpain 10