title: Determination and pharmacokinetic study of pirfenidone in rat plasma by UPLC-MS/MS.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed and validated for the determination and pharmacokinetic investigation of pirfenidone in rat plasma. Sample preparation was accomplished through a simple one-step deproteinization procedure with 0.2 mL of acetonitrile to a 0.1 mL plasma sample. Plasma samples were separated by UPLC on an Acquity UPLC BEH C18 column using a mobile phase consisting of acetonitrile-0.1% formic acid in water with gradient elution. The total run time was 3.0 min and the elution of pirfenidone was at 1.39 min. The detection was performed on a triple quadrupole tandem mass spectrometer in the multiple reaction-monitoring (MRM) mode using the respective transitions m/z 186.2→92.1 for pirfenidone and m/z 237.1→194.2 for carbamazepine (IS), respectively. The calibration curve was linear over the range of 5-2000 ng/mL with a lower limit of quantitation (LLOQ) of 5 ng/mL. Mean recovery of pirfenidone in plasma was in the range of 80.4-84.3%. Intra-day and inter-day precision were both <12.1%. This method was successfully applied in pharmacokinetic study after oral administration of 10.0mg/kg pirfenidone in rats.
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Zhe-li
authors.initials: ZL
authors.lastname: Jiang
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Rui-min
authors.initials: RM
authors.lastname: Chen
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Ningbo Municipal Hospital of TCM, Ningbo 315010, China.
authors.forename: Wan-shu
authors.initials: WS
authors.lastname: Li
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China.
authors.forename: Shuo-min
authors.initials: SM
authors.lastname: Jiang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
authors.forename: Guo-xin
authors.initials: GX
authors.lastname: Hu
authors.affiliation: The Second Affiliated Hospital & Yuying Children' s Hospital of Wenzhou Medical University, Wenzhou 325027, China. Electronic address: crj@wzhealth.com.
authors.forename: Rui-jie
authors.initials: RJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D057230:Limit of Detection; D008297:Male; D011728:Pyridones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011728:Pyridones; C093844:pirfenidone
keywords: Pharmacokinetics; Pirfenidone; Rat plasma; UPLC–MS/MS