title: Determination of phosphatidylserine in milk-based nutritional products using online derivatization high-performance liquid chromatography.
abstract: Phosphatidylserine (PS) has received interest for its ability to improve cognitive abilities and behaviors. A new method for determining PS in milk-based nutritional products has been developed. The method includes a quick and simple sample preparation procedure, followed by high-performance liquid chromatography (HPLC) fluorescence detection (FLD) with an on-line 9-fluorenylmethyloxycarbonyl (FMOC) derivatization. The method allows PS to be determined in raw materials, milk powder and liquid milk products. The day-to-day (n=3 days) average recovery of over spike-in (at 100% PS content level) was 100%, and the method quantification limit is 53 mg per kg milk powder.
authors.affiliation: Abbott Nutrition Research and Development, 20 Biopolis Way #09-01/02, 138668, Singapore. Electronic address: qi.lin@abbott.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Abbott Nutrition Research and Development, 20 Biopolis Way #09-01/02, 138668, Singapore.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Abbott Nutrition Research and Development, 20 Biopolis Way #09-01/02, 138668, Singapore.
authors.forename: Weijie
authors.initials: W
authors.lastname: Pei
authors.affiliation: Abbott Nutrition Research and Development, 20 Biopolis Way #09-01/02, 138668, Singapore.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Abbott Nutrition Research and Development, 20 Biopolis Way #09-01/02, 138668, Singapore.
authors.forename: Jia Le
authors.initials: JL
authors.lastname: Yew
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003611:Dairy Products / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D010718:Phosphatidylserines / Q000032:analysis*
chemical_list: D010718:Phosphatidylserines
keywords: Fluorescence detection; Liquid chromatography; Milk; Online derivatization; Phosphatidylserine