title: High-efficiency in vitro and in vivo detection of Zn2+ by dye-assembled upconversion nanoparticles.
abstract: Development of highly sensitive and selective sensing systems of divalent zinc ion (Zn(2+)) in organisms has been a growing interest in the past decades owing to its pivotal role in cellular metabolism, apoptosis, and neurotransmission. Herein, we report the rational design and synthesis of a Zn(2+) fluorescent-based probe by assembling lanthanide-doped upconversion nanoparticles (UCNPs) with chromophores. Specifically, upconversion luminescence (UCL) can be effectively quenched by the chromophores on the surface of nanoparticles via a fluorescence resonant energy transfer (FRET) process and subsequently recovered upon the addition of Zn(2+), thus allowing for quantitative monitoring of Zn(2+). Importantly, the sensing system enables detection of Zn(2+) in real biological samples. We demonstrate that this chromophore-UCNP nanosystem is capable of implementing an efficient in vitro and in vivo detection of Zn(2+) in mouse brain slice with Alzheimer's disease and zebrafish, respectively.
authors.affiliation: Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A* STAR), 138667, Singapore.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Peng
authors.forename: Wang
authors.initials: W
authors.lastname: Xu
authors.forename: Chai Lean
authors.initials: CL
authors.lastname: Teoh
authors.forename: Sanyang
authors.initials: S
authors.lastname: Han
authors.forename: Beomsue
authors.initials: B
authors.lastname: Kim
authors.forename: Animesh
authors.initials: A
authors.lastname: Samanta
authors.forename: Jun Cheng
authors.initials: JC
authors.lastname: Er
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Yuan
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D053758:Nanoparticles; D015032:Zinc / Q000032:analysis*
chemical_list: D004396:Coloring Agents; D015032:Zinc