title: Effect of ohmic heating of soymilk on urease inactivation and kinetic analysis in holding time.
abstract: To verify the effect of the ohmic heating on the urease activity in the soymilk, the ohmic heating methods with the different electrical field conditions (the frequency and the voltage ranging from 50 to 10 kHz and from 160 to 220 V, respectively) were employed. The results showed that if the value of the urease activity measured with the quantitative spectrophotometry method was lower than 16.8 IU, the urease activity measured with the qualitative method was negative. The urease activity of the sample ohmically heated was significantly lower than that of the sample conventionally heated (P < 0.01) at the same target temperature. It was concluded that the electrical field enhanced the urease inactivation. In addition, the inactivation kinetics of the urease in the soymilk could be described with a biphasic model during holding time at a target temperature. Thus, it was concluded that the urease in the soymilk would contain 2 isoenzymes, one is the thermolabile fraction, the other the thermostable fraction, and that the thermostable isoenzyme could not be completely inactivated when the holding time increased, whether the soymilk was cooked with the conventional method or with the ohmic heating method. Therefore, the electric field had no effect on the inactivation of the thermostable isoenzyme of the urease.
authors.affiliation: College of Mechanical and Electronic Engineering, Shandong Agricultural Univ, Tai'an, 271018, China; Shandong Provincial Key Laboratory of Horticultural Machinery and Equipment, Tai'an, 271018, China; College of Food Science and Engineering, Shandong Agricultural Univ, Tai'an, 271018, China.
authors.forename: Fa-De
authors.initials: FD
authors.lastname: Li
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Ren
authors.forename: Ranran
authors.initials: R
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
mesh_terms: D004560:Electricity; D005511:Food Handling / Q000379:methods*; D006361:Heating; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D011489:Protein Denaturation; D041942:Soy Milk / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry; D014510:Urease / Q000737:chemistry*
chemical_list: D014510:Urease
keywords: biphasic model; isoenzyme; ohmic heating; soymilk; urease activity