title: High-throughput detection of DNA double-strand breaks using image cytometry.
abstract: Assessment of γH2AX expression for studying DNA double-strand break formation is often performed by manual counting of foci using immunofluorescence microscopy, an approach that is laborious and subject to significant foci selection bias. Here we present a novel high-throughput method for detecting DNA double-strand breaks using automated image cytometry assessment of cell average γH2AX immunofluorescence. Our technique provides an expedient, high-throughput, objective, and cost-effective method for γH2AX analysis.
authors.affiliation: Departments of Medical Physics, School of Medicine and Public Health, University of Wisconsin, Madison, WI.
authors.forename: Tyler L
authors.initials: TL
authors.lastname: Fowler
authors.affiliation: Departments of Human Oncology, School of Medicine and Public Health, University of Wisconsin, Madison, WI.
authors.forename: Alison M
authors.initials: AM
authors.lastname: Bailey
authors.affiliation: Departments of Medical Physics, School of Medicine and Public Health, University of Wisconsin, Madison, WI; University of Wisconsin Carbone Comprehensive Cancer Center, School of Medicine and Public Health, University of Wisconsin, Madison, WI.
authors.forename: Bryan P
authors.initials: BP
authors.lastname: Bednarz
authors.affiliation: Departments of Human Oncology, School of Medicine and Public Health, University of Wisconsin, Madison, WI; University of Wisconsin Carbone Comprehensive Cancer Center, School of Medicine and Public Health, University of Wisconsin, Madison, WI.
authors.forename: Randall J
authors.initials: RJ
authors.lastname: Kimple
mesh_terms: D018929:Cell Culture Techniques; D053903:DNA Breaks, Double-Stranded; D004260:DNA Repair; D006367:HeLa Cells; D006801:Humans; D019044:Image Cytometry / Q000379:methods*
keywords: DNA; double-strand breaks; image cytometry; γH2AX