title: Dual functional graphene derivative-based electrochemical platforms for detection of the TP53 gene with single nucleotide polymorphism selectivity in biological samples.
abstract: Novel disposable electrochemical DNA sensors were prepared for the detection of a target DNA sequence on the p53 tumor suppressor (TP53) gene. The electrochemical platform consisted of screen-printed carbon electrodes (SPCEs) functionalized with a water-soluble reduced graphene oxide-carboxymethylcellulose (rGO-CMC) hybrid nanomaterial. Two different configurations involving hairpin specific capture probes of different length covalently immobilized through carbodiimide chemistry on the surface of rGO-CMC-modified SPCEs were implemented and compared. Upon hybridization, a streptavidin-peroxidase (Strep-HRP) conjugate was employed as an electrochemical indicator. Hybridization was monitored by recording the amperometric responses measured at -0.10 V (vs an Ag pseudo-reference electrode) upon the addition of 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator and H2O2 as an enzyme substrate. The implemented DNA platforms allow single nucleotide polymorphism (SNP) discrimination in cDNAs from human breast cancer cell lines, which makes such platforms excellent as new diagnosis tools in clinical analysis.
authors.affiliation: Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid , E-28040 Madrid, Spain.
authors.forename: Berta
authors.initials: B
authors.lastname: Esteban-Fernández de Ávila
authors.forename: Elena
authors.initials: E
authors.lastname: Araque
authors.forename: Susana
authors.initials: S
authors.lastname: Campuzano
authors.forename: María
authors.initials: M
authors.lastname: Pedrero
authors.forename: Berna
authors.initials: B
authors.lastname: Dalkiran
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Barderas
authors.forename: Reynaldo
authors.initials: R
authors.lastname: Villalonga
authors.forename: Esma
authors.initials: E
authors.lastname: Kiliç
authors.forename: José M
authors.initials: JM
authors.lastname: Pingarrón
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry; D010087:Oxides / Q000737:chemistry; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D010087:Oxides; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D006108:Graphite; D002266:Carboxymethylcellulose Sodium