title: Image-based prediction of drug target in yeast.
abstract: Discovering the intracellular target of drugs is a fundamental challenge in biomedical research. We developed an image-based technique with which we were able to identify intracellular target of the compounds in the yeast Saccharomyces cerevisiae. Here, we describe the rationale of the technique, staining of yeast cells, image acquisition, data processing, and statistical analysis required for prediction of drug targets.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg. FSB-101, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277-8562, Japan.
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Ohnuki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Okada
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Ohya
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008826:Microbial Sensitivity Tests; D008853:Microscopy / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents