title: Glycerol salicylate-based containing α-tricalcium phosphate as a bioactive root canal sealer.
abstract: The use of bioactive materials instead of inert materials to fill the root canal space could be an effective approach to achieve a hermetic seal and stimulate the healing of periapical tissues. The purpose of this study was to develop and characterize an endodontic sealer based on a glycerol salicylate resin and α-tricalcium phosphate (αTCP) at physical and chemical properties. Different sealers were formulated using 70% of a glycerol salicylate resin and 30% of a mixture of calcium hydroxide and αTCP (0, 5, 10, or 15%, in weight). Sealers formulated were characterized based on setting time, in vitro degradation over time, pH, cytotoxicity, and mineral deposition. Sealers presented setting time ranging from 240 to 405 min, and basic pH over 8.21 after 28 days. Higher αTCP concentration leads to sealers with low solubility. Cell viability after 48 h in direct contact with sealers was similar to a commercial sealer used as reference. The 10% and 15% αTCP sealers exhibited a calcium-phosphate layer on the surface after immersion in water and SBF for 7 days. Glycerol salicylate sealers with 10% and 15% α-tricalcium phosphate showed reliable physical-chemical properties and apatite-forming ability.
authors.affiliation: Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fernando F
authors.initials: FF
authors.lastname: Portella
authors.affiliation: Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Fabrício M
authors.initials: FM
authors.lastname: Collares
authors.affiliation: Biomaterials Laboratory, Department of Materials, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Luís A
authors.initials: LA
authors.lastname: Dos Santos
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Bruno P
authors.initials: BP
authors.lastname: dos Santos
authors.affiliation: Laboratory of Stem Cells and Tissue Engineering, Universidade Luterana do Brasil, Canoas, RS, Brazil.
authors.forename: Melissa
authors.initials: M
authors.lastname: Camassola
authors.affiliation: Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Vicente C B
authors.initials: VC
authors.lastname: Leitune
authors.affiliation: Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Susana M W
authors.initials: SM
authors.lastname: Samuel
mesh_terms: D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005995:Glyceryl Ethers / Q000737:chemistry / Q000494:pharmacology*; D008422:Materials Testing; D051379:Mice; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000494:pharmacology*; D012459:Salicylates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002130:Calcium Phosphates; D005995:Glyceryl Ethers; D012387:Root Canal Filling Materials; D012459:Salicylates; C485828:alpha-tricalcium phosphate
keywords: Raman; bioactive sealers; glycerol; salicylate; α-tricalcium phosphate