title: Phytochemical profile and bioactivity of traditional ayurvedic decoctions and hydro-alcoholic macerations of Boerhaavia diffusa L. and Curculigo orchioides Gaertn.
abstract: Decoctions (DECs) and hydro-alcoholic extracts (HEs) prepared from roots of Boerhaavia diffusa L. (Nyctaginaceae) and Curculigo orchioides Gaertn. (Hypoxidaceae) were phytochemically characterised by HPLC-DAD and profiled for their antioxidant, antigenotoxic and cytotoxic activities. B. diffusa DEC was rich in ferulic acid and vanillin, while the HE also contained boeravinone B and eupalitin. Both C. orchioides HE and DEC displayed the main occurrence of orcinol-β-d-glucoside and curculigoside A. Antioxidant activity was assayed through spectrophotometric DPPH, ABTS and β-carotene bleaching test, and using (HP)TLC bioautographic strategies. For both crude drugs, HE was the best performing preparation. Properly modified SOS-Chromotest evidenced a 10% inhibition by phytocomplexes against 4-nitroquinoline-N-oxide, and a higher bioactivity for vanillin (36.60 ± 1.68%) and ferulic acid (35.09 ± 1.53%). C. orchioides HE was the preparation which showed higher cytotoxicity against drug-sensitive human T-lymphoblastoid cell line (CCRF-CEM) and multidrug-resistant leukaemia cell line (CEM/ADR5000), and eupalitin was the only pure compound to exhibit an IC50 value.
authors.affiliation: a Department of Life Sciences and Biotechnology (SVeB) , University of Ferrara , C.so Ercole I d'Este 32, 44121 Ferrara , Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Tacchini
authors.affiliation: a Department of Life Sciences and Biotechnology (SVeB) , University of Ferrara , C.so Ercole I d'Este 32, 44121 Ferrara , Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Spagnoletti
authors.affiliation: b Department of Food Science , University of Parma , Viale G.P. Usberti 95a, 43123 Parma , Italy.
authors.forename: Matteo
authors.initials: M
authors.lastname: Marieschi
authors.affiliation: b Department of Food Science , University of Parma , Viale G.P. Usberti 95a, 43123 Parma , Italy.
authors.forename: Augusta
authors.initials: A
authors.lastname: Caligiani
authors.affiliation: b Department of Food Science , University of Parma , Viale G.P. Usberti 95a, 43123 Parma , Italy.
authors.forename: Renato
authors.initials: R
authors.lastname: Bruni
authors.affiliation: c Department of Pharmaceutical Biology , Institute of Pharmacy and Biochemistry, Johannes Gutenberg University , Staudinger Weg 5, 55128 Mainz , Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Efferth
authors.affiliation: a Department of Life Sciences and Biotechnology (SVeB) , University of Ferrara , C.so Ercole I d'Este 32, 44121 Ferrara , Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sacchetti
authors.affiliation: a Department of Life Sciences and Biotechnology (SVeB) , University of Ferrara , C.so Ercole I d'Este 32, 44121 Ferrara , Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Guerrini
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D045744:Cell Line, Tumor; D031408:Curculigo / Q000737:chemistry*; D006801:Humans; D008515:Medicine, Ayurvedic; D039281:Nyctaginaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Boerhaavia diffusa; Curculigo orchioides; antioxidant activity; chemical fingerprinting; cytotoxicity; genotoxicity; traditional preparations