title: Atrioventricular node ablation in Langendorff-perfused porcine hearts using carbon ion particle therapy: methods and an in vivo feasibility investigation for catheter-free ablation of cardiac arrhythmias.
abstract: BACKGROUND
  Particle therapy, with heavy ions such as carbon-12 ((12)C), delivered to arrhythmogenic locations of the heart could be a promising new means for catheter-free ablation. As a first investigation, we tested the feasibility of in vivo atrioventricular node ablation, in Langendorff-perfused porcine hearts, using a scanned 12C beam.
  
  
  RESULTS
  Intact hearts were explanted from 4 (30-40 kg) pigs and were perfused in a Langendorff organ bath. Computed tomographic scans (1 mm voxel and slice spacing) were acquired and (12)C ion beam treatment planning (optimal accelerator energies, beam positions, and particle numbers) for atrioventricular node ablation was conducted. Orthogonal x-rays with matching of 4 implanted clips were used for positioning. Ten Gray treatment plans were repeatedly administered, using pencil beam scanning. After delivery, positron emission tomography-computed tomographic scans for detection of β(+) ((11)C) activity were obtained. A (12)C beam with a full width at half maximum of 10 mm was delivered to the atrioventricular node. Delivery of 130 Gy caused disturbance of atrioventricular conduction with transition into complete heart block after 160 Gy. Positron emission computed tomography demonstrated dose delivery into the intended area. Application did not induce arrhythmias. Macroscopic inspection did not reveal damage to myocardium. Immunostaining revealed strong γH2AX signals in the target region, whereas no γH2AX signals were detected in the unirradiated control heart.
  
  
  CONCLUSIONS
  This is the first report of the application of a (12)C beam for ablation of cardiac tissue to treat arrhythmias. Catheter-free ablation using 12C beams is feasible and merits exploration in intact animal studies as an energy source for arrhythmia elimination.
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.). helge.i.lehmann@gmail.com.
authors.forename: H Immo
authors.initials: HI
authors.lastname: Lehmann
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Daniel
authors.initials: D
authors.lastname: Richter
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Hannah
authors.initials: H
authors.lastname: Prokesch
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Christian
authors.initials: C
authors.lastname: Graeff
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Matthias
authors.initials: M
authors.lastname: Prall
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Palma
authors.initials: P
authors.lastname: Simoniello
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Claudia
authors.initials: C
authors.lastname: Fournier
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Julia
authors.initials: J
authors.lastname: Bauer
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Robert
authors.initials: R
authors.lastname: Kaderka
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Alexander
authors.initials: A
authors.lastname: Weymann
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Gábor
authors.initials: G
authors.lastname: Szabó
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Karin
authors.initials: K
authors.lastname: Sonnenberg
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Anna M
authors.initials: AM
authors.lastname: Constantinescu
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Susan B
authors.initials: SB
authors.lastname: Johnson
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Juna
authors.initials: J
authors.lastname: Misiri
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Takami
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Robert C
authors.initials: RC
authors.lastname: Miller
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Michael G
authors.initials: MG
authors.lastname: Herman
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Asirvatham
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Stephan
authors.initials: S
authors.lastname: Brons
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Oliver
authors.initials: O
authors.lastname: Jäkel
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Thomas
authors.initials: T
authors.lastname: Haberer
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Jürgen
authors.initials: J
authors.lastname: Debus
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Marco
authors.initials: M
authors.lastname: Durante
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Christoph
authors.initials: C
authors.lastname: Bert
authors.affiliation: From the Mayo Clinic Translational Interventional Electrophysiology Laboratory (H.I.L., S.B.J., J.M., M.T., S.J.A., D.L.P.) and Department of Radiation Oncology (R.C.M., M.G.H.), Mayo Clinic, Rochester, MN; Department of Biophysics, GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany (D.R., C.G., M.P., P.S., C.F., R.K., A.M.C., M.D., C.B.); Department of Radiation Oncology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany (D.R., C.B.); Heidelberg Ion-Beam Therapy Center (HIT), Heidelberg, Germany (H.P., J.B., S.B., T.H., J.D.); Department of Experimental Cardiothoracic Surgery, University of Heidelberg, Heidelberg, Germany (A.W., G.S., K.S.); and German Cancer Research Center (DKFZ), Heidelberg, Germany (O.J.).
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Packer
mesh_terms: D055011:Ablation Techniques / Q000009:adverse effects / Q000295:instrumentation; D000818:Animals; D001283:Atrioventricular Node / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology / Q000528:radiation effects*; D022062:Electrophysiologic Techniques, Cardiac; D005240:Feasibility Studies; D057918:Fiducial Markers; D006339:Heart Rate / Q000528:radiation effects; D063193:Heavy Ion Radiotherapy / Q000009:adverse effects / Q000295:instrumentation; D006657:Histones / Q000378:metabolism; D023421:Models, Animal; D064847:Multimodal Imaging; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D010477:Perfusion; D049268:Positron-Emission Tomography; D011879:Radiotherapy Dosage; D011880:Radiotherapy Planning, Computer-Assisted; D011881:Radiotherapy, Computer-Assisted; D034421:Sus scrofa; D014057:Tomography, X-Ray Computed
chemical_list: D006657:Histones
keywords: atrial fibrillation; atrioventricular node; carbon particle therapy; catheter-free ablation; tachycardia, ventricular