title: DNA-based vaccination against hepatitis B virus using dissolving microneedle arrays adjuvanted by cationic liposomes and CpG ODN.
abstract: DNA vaccines are simple to produce and can generate strong cellular and humoral immune response, making them attractive vaccine candidates. However, a major shortcoming of DNA vaccines is their poor immunogenicity when administered intramuscularly. Transcutaneous immunization (TCI) via microneedles is a promising alternative delivery route to enhance the vaccination efficacy. A novel dissolving microneedle array (DMA)-based TCI system loaded with cationic liposomes encapsulated with hepatitis B DNA vaccine and adjuvant CpG ODN was developed and characterized. The pGFP expression in mouse skin using DMA was imaged over time. In vivo immunity tests in mice were performed to observe the capability of DMA to induce immune response after delivery of DNA. The results showed that pGFP could be delivered into skin by DMA and expressed in skin. Further, the amount of expressed GFP was likely to peak at day 4. The immunity tests showed that the DMA-based DNA vaccination could induce effective immune response. CpG ODN significantly improved the immune response and achieved the shift of immune type from predominate Th2 type to a balance Th1/Th2 type. The cationic liposomes could further improve the immunogenicity of DNA vaccine. In conclusion, the novel DMA-based TCI system can effectively deliver hepatitis B DNA vaccine into skin, inducing effective immune response and change the immune type by adjuvant CpG ODN.
authors.affiliation: a Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Haidian , Beijing , China , and.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: a Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Haidian , Beijing , China , and.
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.affiliation: a Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Haidian , Beijing , China , and.
authors.forename: Suohui
authors.initials: S
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Haidian , Beijing , China , and.
authors.forename: Bai
authors.initials: B
authors.lastname: Xu
authors.affiliation: a Key Laboratory of Photochemical Conversion and Optoelectronic Materials , Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , Haidian , Beijing , China , and.
authors.forename: Yunhua
authors.initials: Y
authors.lastname: Gao
authors.affiliation: b 302 Military Hospital of China , Fengtai , Beijing , China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Hu
authors.affiliation: b 302 Military Hospital of China , Fengtai , Beijing , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Hou
authors.affiliation: b 302 Military Hospital of China , Fengtai , Beijing , China.
authors.forename: Bingke
authors.initials: B
authors.lastname: Bai
authors.affiliation: b 302 Military Hospital of China , Fengtai , Beijing , China.
authors.forename: Honghui
authors.initials: H
authors.lastname: Shen
authors.affiliation: b 302 Military Hospital of China , Fengtai , Beijing , China.
authors.forename: Panyong
authors.initials: P
authors.lastname: Mao
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage* / Q000737:chemistry; D000279:Administration, Cutaneous; D000818:Animals; D002412:Cations / Q000737:chemistry*; D004247:DNA / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D017325:Hepatitis B Vaccines / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology; D006515:Hepatitis B virus / Q000737:chemistry* / Q000378:metabolism; D056724:Immunity, Humoral / Q000187:drug effects*; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D012867:Skin / Q000187:drug effects*
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D002412:Cations; D017325:Hepatitis B Vaccines; D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D004247:DNA
keywords: Adjuvants; DNA vaccine; dissolving microneedle arrays; hepatitis B virus; transcutaneous immunization