title: Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus.
abstract: BACKGROUND
  Type 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis.
  
  
  OBJECTIVE
  Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies from the same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles.
  
  
  RESULTS
  In the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95% CI = 0.8011-1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95% CI = 0.7585-1.667; p = 0.5606) respectively.
  
  
  CONCLUSIONS
  Our study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad 500068, India ; Department of Genetics, Vasavi Medical and Research Centre, Lakdikapool, Hyderabad 500004, India ; Department of Genetics and Biotechnology, Osmania University, Tarnaka, Hyderabad 500007, India.
authors.forename: Imran Ali
authors.initials: IA
authors.lastname: Khan
authors.affiliation: Department of Genetics, Vasavi Medical and Research Centre, Lakdikapool, Hyderabad 500004, India.
authors.forename: Sireesha
authors.initials: S
authors.lastname: Movva
authors.affiliation: Department of Genetic Medicine, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Noor Ahmad
authors.initials: NA
authors.lastname: Shaik
authors.affiliation: Department of Genetics, Vasavi Medical and Research Centre, Lakdikapool, Hyderabad 500004, India.
authors.forename: Srinivas
authors.initials: S
authors.lastname: Chava
authors.affiliation: Department of Genetics and Biotechnology, Osmania University, Tarnaka, Hyderabad 500007, India.
authors.forename: Parveen
authors.initials: P
authors.lastname: Jahan
authors.affiliation: Department of Nephrology, Kamineni Hospitals, LB Nagar, Hyderabad 500068, India.
authors.forename: Kamal Kiran
authors.initials: KK
authors.lastname: Mukkavali
authors.affiliation: Department of Gynecology, Kamineni Hospitals, LB Nagar, Hyderabad 500068, India.
authors.forename: Vasundhara
authors.initials: V
authors.lastname: Kamineni
authors.affiliation: Department of Genetics and Molecular Medicine, Kamineni Hospitals, LB Nagar, Hyderabad 500068, India ; Department of Genetics, Vasavi Medical and Research Centre, Lakdikapool, Hyderabad 500004, India.
authors.forename: Qurratulain
authors.initials: Q
authors.lastname: Hasan
authors.affiliation: Department of Biochemistry, Kamineni Hospitals, LB Nagar, Hyderabad 500068, India ; Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal 576104, Karnataka, India.
authors.forename: Pragna
authors.initials: P
authors.lastname: Rao
keywords: Asian Indians; CAPN10; Gestational Diabetes Mellitus (GDM); PCR–RFLP; Type 2 Diabetes Mellitus (T2DM)