title: Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide ofLarimichthys croceaswim bladder.
abstract: Larimichthys croceaswim bladder is a traditional food and medicine widely used in China. Thein vitroanticancer effects of polysaccharide ofL. croceaswim bladder (PLCSB) in HCT-116 human colon cancer cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 μg/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration-dependent manner. In particular, 400 μg/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6-diamidino-2-phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT-116 cancer cells, the expression of apoptosis and metastasis-associated genes was analyzed by reverse transcription-polymerase chain reaction and western blot analysis. A total of 400 μg/ml PLCSB significantly induced apoptosis in HCT-116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase-3, -8, and -9, as well as Fas and the downregulation of B-cell lymphoma 2 (Bcl-2), Bcl-extra large and Fas ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT-116 colon cancer cells,in vitro.
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, P.R. China.
authors.forename: Huayi
authors.initials: H
authors.lastname: Suo
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, P.R. China ; Department of Food Science and Nutrition, Pusan National University, Busan 609735, Republic of Korea.
authors.forename: Jia-LE
authors.initials: JL
authors.lastname: Song
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Yalin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Science and Technology Administration, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China.
authors.forename: Zhenhu
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Food Science and Nutrition, Pusan National University, Busan 609735, Republic of Korea.
authors.forename: Ruokun
authors.initials: R
authors.lastname: Yi
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhu
authors.affiliation: College of Food Science, Southwest University, Chongqing 400715, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China ; Institute of Functional Ecological Food, Chongqing University of Education, Chongqing 400067, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
keywords: HCT-116 human colon cancer cells; Larimichthys crocea swim bladder; apoptosis; gene; polysaccharide