title: Influences of gestational obesity on associations between genotypes and gene expression levels in offspring following maternal gastrointestinal bypass surgery for obesity.
abstract: METHODS
  Whole-genome genotyping and gene expression analyses in blood of 22 BMS and 23 AMS offspring from 19 mothers were conducted using Illumina HumanOmni-5-Quad and HumanHT-12 v4 Expression BeadChips, respectively. Using PLINK we analyzed interactions between offspring gene variations and maternal surgical status on offspring gene expression levels. Altered biological functions and pathways were identified and visualized using DAVID and Ingenuity Pathway Analysis.
  
  
  RESULTS
  Significant interactions (p ≤ 1.22 x 10(-12)) were found for 525 among the 16,060 expressed transcripts: 1.9% of tested SNPs were involved. Gene function and pathway analysis demonstrated enrichment of transcription and of cellular metabolism functions and overrepresentation of cellular stress and signaling, immune response, inflammation, growth, proliferation and development pathways.
  
  
  CONCLUSIONS
  We suggest that impaired maternal gestational metabolic fitness interacts with offspring gene variations modulating gene expression levels, providing potential mechanisms explaining improved cardiometabolic risk profiles of AMS offspring related to ameliorated maternal lipid and carbohydrate metabolism.
authors.affiliation: Institute of Nutrition and Functional Foods (INAF) and Department of Food Science and Nutrition, Laval University, Quebec, Canada; Endocrinology and Nephrology, CHU de Quebec Research Center, Quebec, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Guénard
authors.affiliation: Quebec Heart and Lung Institute, Quebec, Canada.
authors.forename: Maxime
authors.initials: M
authors.lastname: Lamontagne
authors.affiliation: Quebec Heart and Lung Institute, Quebec, Canada; Department of Molecular Medicine, Laval University, Quebec, Canada.
authors.forename: Yohan
authors.initials: Y
authors.lastname: Bossé
authors.affiliation: Quebec Heart and Lung Institute, Quebec, Canada; Department of Medicine, Laval University, Quebec, Canada.
authors.forename: Yves
authors.initials: Y
authors.lastname: Deshaies
authors.affiliation: Quebec Heart and Lung Institute, Quebec, Canada; Department of Medicine, Laval University, Quebec, Canada.
authors.forename: Katherine
authors.initials: K
authors.lastname: Cianflone
authors.affiliation: Department of Surgery, SUNY Downstate Medical Center, Brooklyn, New York, United States of America.
authors.forename: John G
authors.initials: JG
authors.lastname: Kral
authors.affiliation: Quebec Heart and Lung Institute, Quebec, Canada; Department of Surgery, Laval University, Quebec, Canada.
authors.forename: Picard
authors.initials: P
authors.lastname: Marceau
authors.affiliation: Institute of Nutrition and Functional Foods (INAF) and Department of Food Science and Nutrition, Laval University, Quebec, Canada; Endocrinology and Nephrology, CHU de Quebec Research Center, Quebec, Canada.
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Vohl
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D009035:Mothers; D009765:Obesity / Q000235:genetics* / Q000601:surgery*; D011247:Pregnancy; D055815:Young Adult