title: Fat/vessel-derived secretory protein (Favine)/CCDC3 is involved in lipid accumulation.
abstract: We previously identified a novel gene encoding Favine/CCDC3 (NCBI protein entry NP_083080), a possible secretory factor, the mRNA of which is highly expressed in adipose tissue and the aorta. The Favine mRNA levels are increased in the course of differentiation of rat primary adipocytes and are more elevated in the adipose tissue of genetically obese and diet-induced obese mice than in lean mice. However, its biological function has not yet been elucidated until now. Here, we tested the hypothesis that Favine is involved in lipid metabolism in adipocytes. We found that overexpression of Favine promoted 3T3-L1 adipocyte differentiation. To further investigate the function of Favine in vivo, we generated Favine knock-out (KO) mice. Favine KO mice exhibited a lean phenotype as they aged. The weights of white adipose tissue and liver were less, and adipocyte size was smaller in Favine KO mice compared with wild-type littermates (WT). Expression levels of lipogenic genes, such as fatty-acid synthase (FAS), acetyl-CoA carboxylase α (ACC1), and diacylglycerol O-acyltransferase-2 (Dgat2), were decreased in adipose tissue of Favine KO mice. In 1-year-old mice, Favine deficiency decreased the number of inflammatory cells in white adipose tissue and diminished hepatic steatosis. In vitro, deficiency of Favine attenuated differentiation of primary adipocytes. Taken together, these data demonstrate that Favine has adipogenic and lipogenic effects on adipocytes.
authors.affiliation: From the Departments of Metabolic Medicine and.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: From the Departments of Metabolic Medicine and fukuhara@endmet.med.osaka-u.ac.jp.
authors.forename: Atsunori
authors.initials: A
authors.lastname: Fukuhara
authors.affiliation: From the Departments of Metabolic Medicine and.
authors.forename: Michio
authors.initials: M
authors.lastname: Otsuki
authors.affiliation: the Departments of Organ Network and Metabolism and.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Suganami
authors.affiliation: Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-545 Yushima, Bunkyo-ku, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ogawa
authors.affiliation: Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka and.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Morii
authors.affiliation: From the Departments of Metabolic Medicine and.
authors.forename: Iichiro
authors.initials: I
authors.lastname: Shimomura
mesh_terms: D041721:3T3-L1 Cells; D000256:Adenoviridae / Q000235:genetics; D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D050154:Adiposity; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002454:Cell Differentiation; D017931:DNA Primers; D005234:Fatty Liver / Q000517:prevention & control; D050356:Lipid Metabolism; D051379:Mice; D018345:Mice, Knockout; D011506:Proteins / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D012190:Retroviridae / Q000235:genetics
chemical_list: C000598213:CCDC3 protein, mouse; D017931:DNA Primers; D011506:Proteins
keywords: Adipogenesis; Adipose Tissue; Coiled-coil Domain Containing 3 Gene; Favine Gene; Hepatic Steatosis; Lipogenesis; Liver; Obesity