title: Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq.
abstract: The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience.
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: TaeHyun
authors.initials: T
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Chae-Seok
authors.initials: CS
authors.lastname: Lim
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-747, Korea.
authors.forename: Bong-Kiun
authors.initials: BK
authors.lastname: Kaang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002846:Chromatography, Affinity / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D009928:Organ Specificity / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*