title: In vitro and in vivo identification of metabolites of magnoflorine by LC LTQ-Orbitrap MS and its potential pharmacokinetic interaction in Coptidis Rhizoma decoction in rat.
abstract: Magnoflorine, an important aporphine alkaloid in Coptidis Rhizoma, is increasingly attracting research attention because of its pharmacological activities. The in vivo and in vitro metabolism of magnoflorine was investigated by LC LTQ-Orbitrap MS. In vivo samples including rat urine, feces, plasma and bile were collected separately after both oral (50 mg kg(-1) ) and intravenous administration (10 mg kg(-1) ) of magnoflorine, along with in vitro samples prepared by incubating magnoflorine with rat intestinal flora and liver microsome. As a result, 12 metabolites were found in biological samples. Phase I metabolites were identified in all biological samples, while phase II metabolites were mainly detected in urine, plasma and bile. In a pharmacokinetic study, rats were not only dosed with magnoflorine via oral (15, 30 and 60 mg kg(-1) ) and intravenous administration (10 mg kg(-1) ) but also dosed with Coptidis Rhizoma decoction (equivalent to 30 mg kg(-1) of magnoflorine) by intragastric administration to investigate the interaction of magnoflorine with the rest of compounds in Coptidis Rhizoma. Studies showed that magnoflorine possessed lower bioavailability and faster absorption and elimination. However, pharmacokinetic parameters altered significantly (p < 0.05) when magnoflorine was administered in Coptidis Rhizoma decoction. Oral gavage of Coptidis Rhizoma decoction decreased the absorption and elimination rates of magnoflorine, which revealed that there existed pharmacokinetic interactions between magnoflorine and the rest of ingredients in Coptidis Rhizoma.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Baojuan
authors.initials: B
authors.lastname: Xue
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Miao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Miao
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Guixia
authors.initials: G
authors.lastname: Sun
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.|School of Pharmacy, Jiamusi University, Jiamusi,  154007, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Su
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Wei
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing,  100102,   China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001060:Aporphines / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics* / Q000652:urine; D000089042:Coptis chinensis; D004365:Drugs, Chinese Herbal / Q000378:metabolism* / Q000493:pharmacokinetics; D005243:Feces / Q000737:chemistry; D008297:Male; D013058:Mass Spectrometry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001060:Aporphines; D004365:Drugs, Chinese Herbal; C107393:Coptidis rhizoma extract; C001670:magnoflorine
keywords: Coptidis Rhizoma decoction; LC LTQ-Orbitrap MS; magnoflorine; metabolites; pharmacokinetic interaction