title: Dependence of brown adipose tissue function on CD36-mediated coenzyme Q uptake.
abstract: Brown adipose tissue (BAT) possesses the inherent ability to dissipate metabolic energy as heat through uncoupled mitochondrial respiration. An essential component of the mitochondrial electron transport chain is coenzyme Q (CoQ). While cells synthesize CoQ mostly endogenously, exogenous supplementation with CoQ has been successful as a therapy for patients with CoQ deficiency. However, which tissues depend on exogenous CoQ uptake as well as the mechanism by which CoQ is taken up by cells and the role of this process in BAT function are not well understood. Here, we report that the scavenger receptor CD36 drives the uptake of CoQ by BAT and is required for normal BAT function. BAT from mice lacking CD36 displays CoQ deficiency, impaired CoQ uptake, hypertrophy, altered lipid metabolism, mitochondrial dysfunction, and defective nonshivering thermogenesis. Together, these data reveal an important new role for the systemic transport of CoQ to BAT and its function in thermogenesis.
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Courtney M
authors.initials: CM
authors.lastname: Anderson
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Melissa
authors.initials: M
authors.lastname: Kazantzis
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Electrical Engineering and Computer Sciences, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Venkatraman
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA 94945, USA.
authors.forename: Renata L S
authors.initials: RL
authors.lastname: Goncalves
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA 94945, USA.
authors.forename: Casey L
authors.initials: CL
authors.lastname: Quinlan
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA 94945, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: Ng
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA 94945, USA.
authors.forename: Martin
authors.initials: M
authors.lastname: Jastroch
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Daniel I
authors.initials: DI
authors.lastname: Benjamin
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Biao
authors.initials: B
authors.lastname: Nie
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Candice
authors.initials: C
authors.lastname: Herber
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: An-Angela Ngoc
authors.initials: AA
authors.lastname: Van
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Park
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Dawee
authors.initials: D
authors.lastname: Yun
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Chan
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Yu
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Peter
authors.initials: P
authors.lastname: Vuong
authors.affiliation: Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2R3, Canada.
authors.forename: Maria
authors.initials: M
authors.lastname: Febbraio
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Daniel K
authors.initials: DK
authors.lastname: Nomura
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA.
authors.forename: Joseph L
authors.initials: JL
authors.lastname: Napoli
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA 94945, USA.
authors.forename: Martin D
authors.initials: MD
authors.lastname: Brand
authors.affiliation: Nutritional Sciences and Toxicology Department, University of California Berkeley, Berkeley, CA 94720, USA. Electronic address: astahl@berkeley.edu.
authors.forename: Andreas
authors.initials: A
authors.lastname: Stahl
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000818:Animals; D001259:Ataxia / Q000235:genetics / Q000378:metabolism; D018955:CD36 Antigens / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000378:metabolism; D028361:Mitochondrial Diseases / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000378:metabolism; D018908:Muscle Weakness / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism; D022722:Thermogenesis / Q000235:genetics / Q000502:physiology; D014451:Ubiquinone / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*
chemical_list: D018955:CD36 Antigens; D024101:Mitochondrial Proteins; D014451:Ubiquinone; D019308:Palmitic Acid