title: Miraflow, Soft Contact Lens Cleaner: Activity Against Acanthamoeba Spp.
abstract: OBJECTIVE
  Miraflow is a cleaner for soft contact lens which contains 20% isopropyl alcohol. The purpose of this study was to determine the activity against Acanthamoeba trophozoites and cysts for Miraflow. In addition, to determine the activity of combined Miraflow and multipurpose solutions (MPSs) against Acanthamoeba cysts.
  
  
  METHODS
  Two simulated-use studies were conducted. The significance in the log reduction in the number of trophozoites and cysts of A. castellanii strains ATCC 50514 and ATCC 50370 or A. polyphaga ATCC 30461 after exposure to Miraflow alone was determined by the Spearman-Karber method. To examine the activity against Acanthamoeba of combined Miraflow and an MPS, the log reduction in the number of cysts after a 1-min exposure to Miraflow followed by a 4-hr exposure to MPS (ReNu fresh) was also determined.
  
  
  RESULTS
  Short-time exposure of 30 sec to Miraflow demonstrated activity against the Acanthamoeba trophozoites. However, a 1-min treatment was only relatively effective (1.1 log reduction) against the cysts of A. castellanii ATCC 50514, but no statistically significant reduction was observed for the cysts of the other 2 strains. The combined use with Miraflow and MPS demonstrated activity against the cysts, and a 3.0, 1.0, or 1.5 log reduction in the numbers was obtained for A. castellanii ATCC 50514, A. castellanii ATCC 50370, and A. polyphaga ATCC 30461, respectively.
  
  
  CONCLUSIONS
  Exposure to combined Miraflow and MPS resulted in reductions in the number of Acanthamoeba cysts.
authors.affiliation: Departments of Ophthalmology and Regenerative Medicine (T.K.), and Ophthalmology (N.H.-W., A.S., T.U., Y.O.), Ehime University School of Medicine, Toon, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Narumi
authors.initials: N
authors.lastname: Higuchi-Watanabe
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shiraishi
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Uno
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D000048:Acanthamoeba / Q000187:drug effects* / Q000302:isolation & purification; D015823:Acanthamoeba Keratitis / Q000517:prevention & control*; D000818:Animals; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D004202:Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D006801:Humans; D053058:Trophozoites / Q000187:drug effects
chemical_list: D018639:Contact Lens Solutions; D004202:Disinfectants