title: An in vitro model of tissue boundary formation for dissecting the contribution of different boundary forming mechanisms.
abstract: During development and in adult tissues separation of phenotypically distinct cell populations is necessary to ensure proper organization and function of tissues and organs. Various phenomena, such as differential adhesion, differential mechanical tension and cell-cell repulsion, are proposed to cause boundary formation. Moreover, emerging evidence suggests that interplay between multiple such phenomena can underlie boundary formation. Boundary-forming mechanisms are commonly studied in vivo in complex embryo models or in vitro using simple model systems not reflective of in vivo boundary complexity. To better elucidate the interplay between multiple boundary formation mechanism, there is therefore a need for more relevant in vitro model systems that allow quantitative and concomitant studies of the multiple changes in cell/tissue behaviour that lead to boundary establishment. Here, we develop such a model using patterned co-cultures of two cell populations. Using a set of quantitative tools, we demonstrate that our approach allows us to study the mechanisms underlying boundary formation. We demonstrate that in our specific system differential mechanical tension and modulation of migratory behavior of cells accompany boundary formation. The design of our in vitro model system will allow researchers to obtain quantitative, integrative mechanistic data facilitating a faster and more thorough understanding of the fundamental principles underlying boundary formation.
authors.affiliation: University of Toronto, Department of Chemical Engineering and Applied Chemistry, 200 College St., Toronto, ON M5S 3E5, Canada. alison.mcguigan@utoronto.ca.
authors.forename: Sahar
authors.initials: S
authors.lastname: Javaherian
authors.forename: Elisa
authors.initials: E
authors.lastname: D'Arcangelo
authors.forename: Benjamin
authors.initials: B
authors.lastname: Slater
authors.forename: Teresa
authors.initials: T
authors.lastname: Zulueta-Coarasa
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Fernandez-Gonzalez
authors.forename: Alison P
authors.initials: AP
authors.lastname: McGuigan
mesh_terms: D002448:Cell Adhesion / Q000502:physiology; D049108:Cell Enlargement; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D048429:Cell Size; D018920:Coculture Techniques / Q000379:methods; D004847:Epithelial Cells / Q000166:cytology* / Q000502:physiology*; D022001:Focal Adhesions / Q000502:physiology*; D006801:Humans; D008560:Membrane Fluidity / Q000502:physiology*; D008954:Models, Biological; D009024:Morphogenesis / Q000502:physiology*; D013314:Stress, Mechanical