title: Fed-batch strategy for enhancing cell growth and C-phycocyanin production of Arthrospira (Spirulina) platensis under phototrophic cultivation.
abstract: The C-phycocyanin generated in blue-green algae Arthrospira platensis is gaining commercial interest due to its nutrition and healthcare value. In this study, the light intensity and initial biomass concentration were manipulated to improve cell growth and C-phycocyanin production of A.platensis in batch cultivation. The results show that low light intensity and high initial biomass concentration led to increased C-phycocyanin accumulation. The best C-phycocyanin productivity occurred when light intensity and initial biomass concentration were 300μmol/m(2)/s and 0.24g/L, respectively. The fed-batch cultivation proved to be an effective strategy to further enhance C-phycocyanin production of A.platensis. The results indicate that C-phycocyanin accumulation not only requires nitrogen-sufficient condition, but also needs other nutrients. The highest C-phycocyanin content (16.1%), production (1034mg/L) and productivity (94.8mg/L/d) were obtained when using fed-batch strategy with 5mM medium feeding.
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China; Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Energy, Xiamen University, Xiamen 361005, China; Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China.
authors.forename: Xianhai
authors.initials: X
authors.lastname: Zeng
authors.affiliation: College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China. Electronic address: jkj@xmu.edu.cn.
authors.forename: Keju
authors.initials: K
authors.lastname: Jing
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D008027:Light; D009584:Nitrogen / Q000378:metabolism; D052817:Phototrophic Processes; D010798:Phycocyanin / Q000096:biosynthesis*; D053545:Spirulina / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D010798:Phycocyanin; D009584:Nitrogen
keywords: Arthrospira platensis; C-phycocyanin (C-PC); Fed-batch; Initial biomass concentration; Light intensity