title: The many faces of packed column supercritical fluid chromatography--a critical review.
abstract: Packed column sub- and supercritical fluid chromatography (SFC) is a versatile separation method: on the one hand the number of parameters acting on the quality of a separation is very large, and the effects of these parameters can be complex (and not always fully understood). But on another hand, due to numerous advantageous properties of CO2-based mobile phases, method development is a fast task. This paper is a review of the main features of SFC, focusing essentially on achiral separations. However, several fundamental aspects discussed here are also relevant to chiral SFC separations. This is not intended to be an extensive review, as the way to practice SFC has somewhat evolved with time. We rather wished to provide an expert opinion on the characteristics of the method, pointing at the sources of difficulty and displaying the wide possibilities that it offers. A large number of selected applications concerning several different areas are also presented.
authors.affiliation: University of Orleans, Institute of Organic and Analytical Chemistry (ICOA), CNRS UMR 7311, Pôle de Chimie, rue de Chartres, BP 6759, 45067 Orléans, France. Electronic address: eric.lesellier@univ-orleans.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Lesellier
authors.affiliation: University of Orleans, Institute of Organic and Analytical Chemistry (ICOA), CNRS UMR 7311, Pôle de Chimie, rue de Chartres, BP 6759, 45067 Orléans, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: West
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000592:standards*; D025924:Chromatography, Supercritical Fluid
keywords: Carbon dioxide; Elution strength; Fluid properties; Retention mechanisms; Subcritical fluid chromatography; Supercritical fluid chromatography