title: Changes of benthic bacteria and meiofauna assemblages during bio-treatments of anthracene-contaminated sediments from Bizerta lagoon (Tunisia).
abstract: Sediments from Bizerta lagoon were used in an experimental microcosm setup involving three scenarios for the bioremediation of anthracene-polluted sediments, namely bioaugmentation, biostimulation, and a combination of both bioaugmentation and biostimulation. In order to investigate the effect of the biotreatments on the benthic biosphere, 16S rRNA gene-based T-RFLP bacterial community structure and the abundance and diversity of the meiofauna were determined throughout the experiment period. Addition of fresh anthracene drastically reduced the benthic bacterial and meiofaunal abundances. The treatment combining biostimulation and bioaugmentation was most efficient in eliminating anthracene, resulting in a less toxic sedimentary environment, which restored meiofaunal abundance and diversity. Furthermore, canonical correspondence analysis showed that the biostimulation treatment promoted a bacterial community favorable to the development of nematodes while the treatment combining biostimulation and bioaugmentation resulted in a bacterial community that advantaged the development of the other meiofauna taxa (copepods, oligochaetes, polychaetes, and other) restoring thus the meiofaunal structure. The results highlight the importance to take into account the bacteria/meiofauna interactions during the implementation of bioremediation treatment.
authors.affiliation: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia. nourelimen@yahoo.fr.|Equipe Environnement et Microbiologie-MELODY Group-UMR CNRS IPREM 5254-IBEAS, Université de Pau et des Pays de l'Adour, Pau, France. nourelimen@yahoo.fr.|Faculty of Sciences of Bizerte, Laboratory of Environment Biomonitoring, Coastal Ecology and Ecotoxicology Unit, University of Carthage, 7021, Zarzouna, Tunisia. nourelimen@yahoo.fr.
authors.forename: Olfa
authors.initials: O
authors.lastname: Ben Said
authors.affiliation: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia.|Laboratoire Ecosystèmes Marins Côtiers, UMR 5119 CNRS-UM2-IFREMER-IRD-ECOSYM, Université Montpellier 2, Montpellier, France.
authors.forename: Hela
authors.initials: H
authors.lastname: Louati
authors.affiliation: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia.|Equipe Environnement et Microbiologie-MELODY Group-UMR CNRS IPREM 5254-IBEAS, Université de Pau et des Pays de l'Adour, Pau, France.
authors.forename: Amel
authors.initials: A
authors.lastname: Soltani
authors.affiliation: Laboratoire Chimie Analytique BioInorganique Environnement-UMR CNRS IPREM 5254-Helioparc, Université de Pau et des Pays de l'Adour, Pau, France.
authors.forename: Hugues
authors.initials: H
authors.lastname: Preud'homme
authors.affiliation: Equipe Environnement et Microbiologie-MELODY Group-UMR CNRS IPREM 5254-IBEAS, Université de Pau et des Pays de l'Adour, Pau, France.
authors.forename: Cristiana
authors.initials: C
authors.lastname: Cravo-Laureau
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR 5119 CNRS-UM2-IFREMER-IRD-ECOSYM, Université Montpellier 2, Montpellier, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Got
authors.affiliation: Laboratoire Ecosystèmes Marins Côtiers, UMR 5119 CNRS-UM2-IFREMER-IRD-ECOSYM, Université Montpellier 2, Montpellier, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Pringault
authors.affiliation: Laboratoire de Biosurveillance de l'Environnement, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisia.
authors.forename: Patricia
authors.initials: P
authors.lastname: Aissa
authors.affiliation: Equipe Environnement et Microbiologie-MELODY Group-UMR CNRS IPREM 5254-IBEAS, Université de Pau et des Pays de l'Adour, Pau, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Duran
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000378:metabolism*; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D017753:Ecosystem; D019015:Geologic Sediments / Q000382:microbiology; D009348:Nematoda; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014416:Tunisia; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000873:Anthracenes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C034020:anthracene
keywords: Anthracene; Bacteria diversity; Bioremediation; Bizerta lagoon; Community structure; Degradation; Meiofauna; Microcosms; PAH degraders