title: Expression of DAB2IP in human trophoblast and its role in trophoblast invasion.
abstract: OBJECTIVE
  DAB2IP is a growth inhibitor present in many types of cancer cells and is associated with epigenetic regulations controlling tumor development. The primary objective of this study is to determine whether DAB2IP participates in the invasion and migration of trophoblasts during placental development.
  
  
  METHODS
  The expressions of DAB2IP in human placentas (10 villi, 18 term placentas and 20 pre-eclampsia placentas) were determined by immunohistochemistry, Western blotting and quantitative RT-PCR. HTR8/SVneo cells were treated with hypoxia-reoxygenation (H/R) to test how DAB2IP expression would affect the invasion and migration of trophoblasts. JEG-3 andHTR8/SVneo cells were treated with 5-aza-2-deoxycytidine (5-aza-dC) to study the role of DAB2IP promoter methylation in trophoblasts.
  
  
  RESULTS
  DAB2IP was strongly expressed in human villi and extravillous trophoblasts as well as in HTR8/SVneo cells, but not in pre-eclampsia placentas. DAB2IP expression increased after H/R treatment, but the invasive and migratory abilities of trophoblasts were reduced. DAB2IP expression in JEG-3 cells also increased after treatment with 5-aza-dC.
  
  
  CONCLUSIONS
  These findings strongly suggest that DAB2IP is an important negative regulator at the maternal-fetal interface during early pregnancy. Excessive oxidative stress can increase DAB2IP expression in trophoblasts. The mechanism of DNA methylation may involve in its function during the development of pathologic pregnancy.
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Nan
authors.initials: N
authors.lastname: Shan
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: Xiao
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Luo
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Nanlin
authors.initials: N
authors.lastname: Yin
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Qinyin
authors.initials: Q
authors.lastname: Deng
authors.affiliation: a Department of Obstetrics and Gynecology , The First Affiliated Hospital of Chongqing Medical University , Chongqing , People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Qi
mesh_terms: D000328:Adult; D002460:Cell Line; D005260:Female; D006801:Humans; D010929:Placentation; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D014327:Trophoblasts / Q000378:metabolism*; D020703:ras GTPase-Activating Proteins / Q000378:metabolism*
chemical_list: C462952:DAB2IP protein, human; D020703:ras GTPase-Activating Proteins
keywords: DAB2IP; DNA methylation; oxidative stress; pre-eclampsia; trophoblast invasion