title: Stromal derived factor-1α in hippocampus radial glial cells in vitro regulates the migration of neural progenitor cells.
abstract: Stromal derived factor-1α (SDF-1α), a critical chemokine that promotes cell homing to target tissues, was presumed to be involved in the traumatic brain injury cortex. In this study, we determined the expression of SDF-1α in the hippocampus after transection of the fimbria fornix (FF). Realtime PCR and ELISA showed that mRNA transcription and SDF-1α proteins increased significantly after FF transection. In vitro, the expression of SDF-1α in radial glial cells (RGCs) incubated with deafferented hippocampus extracts was observed to be greater than in those incubated with normal hippocampus extracts. The co-culture of neural progenitor cells (NPCs) and RGCs indicated that the extracts of deafferented hippocampus induced more NPCs migrating toward RGCs than the normal extracts. Suppression or overexpression of SDF-1α in RGCs markedly either decreased or increased, respectively, the migration of NPCs. These results suggest that after FF transection, SDF-1α in the deafferented hippocampus was upregulated and might play an important role in RGC induction of NPC migration; therefore, SDF-1α is a target for additional research for determining new therapy for brain injuries.
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Jin
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Lin-Qing
authors.initials: LQ
authors.lastname: Zou
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Zhang
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Hao-Ming
authors.initials: HM
authors.lastname: Li
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Xue-Lei
authors.initials: XL
authors.lastname: Tao
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Jian-Bing
authors.initials: JB
authors.lastname: Qin
authors.affiliation: Department of Anatomy and Neurobiology, The Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, 226001, People's Republic of China.
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tian
mesh_terms: D000818:Animals; D002465:Cell Movement; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D063928:Ependymoglial Cells / Q000378:metabolism*; D005260:Female; D005453:Fluorescence; D020712:Fornix, Brain / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D006624:Hippocampus / Q000166:cytology*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D054377:Chemokine CXCL12; D012333:RNA, Messenger
keywords: SDF-1α; hippocampus; migration; neural progenitor cells; radial glia cells