title: Bioactive fish collagen/polycaprolactone composite nanofibrous scaffolds fabricated by electrospinning for 3D cell culture.
abstract: One of the most challenging objectives of 3D cell culture is the development of scaffolding materials with outstanding biocompatibility and favorable mechanical strength. In this study, we fabricated a novel nanofibrous scaffold composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibrous scaffolds were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreased as FC content was increased in the FC/PCL composite nanofibers. The cytocompatibility of the FC/PCL scaffolds was evaluated by SEM, WST-1 assay, confocal microscopy, western blot, and RT-PCR. It was found that the scaffolds not only facilitated the adhesion, spreading, protrusions, and proliferation of thymic epithelial cells (TECs), but also stimulated the expression of genes and proteins involved in cell adhesion and T-cell development. Thus, these results suggest that the FC/PCL composite nanofibrous scaffolds will be a useful model of 3D cell culture for TECs and may have wide applicability in the future for engineering tissues or organs.
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Da Jeong
authors.initials: DJ
authors.lastname: Choi
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Seung Mi
authors.initials: SM
authors.lastname: Choi
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Hae Yeong
authors.initials: HY
authors.lastname: Kang
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Min
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Rira
authors.initials: R
authors.lastname: Lee
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ikram
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Fazli
authors.initials: F
authors.lastname: Subhan
authors.affiliation: Department of Mechanical Engineering, Korea Polytechnic University, Siheung 429-793, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Song Wan
authors.initials: SW
authors.lastname: Jin
authors.affiliation: Department of Mechanical Engineering, Kyungpook National University, Daegu 702-701, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Young Hun
authors.initials: YH
authors.lastname: Jeong
authors.affiliation: Department of Biochemistry, School of Medicine, Dong-A University, Busan 602-714, Republic of Korea; Pioneer Research Center, Republic of Korea.
authors.forename: Jong-Young
authors.initials: JY
authors.lastname: Kwak
authors.affiliation: Department of Anatomy, Pusan National University School of Medicine, Yangsan, Gyeongsangnam-do, 626-870, Republic of Korea; Pioneer Research Center, Republic of Korea. Electronic address: sikyoon@pusan.ac.kr.
authors.forename: Sik
authors.initials: S
authors.lastname: Yoon
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D004847:Epithelial Cells / Q000166:cytology; D005399:Fishes / Q000378:metabolism*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry*; D013950:Thymus Gland / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen
keywords: 3D cell culture; Composite scaffold; Electrospun nanofiber; Fish collagen; Thymic epithelial cells