title: Novel multiplex format of an extended multilocus variable-number-tandem-repeat analysis of Clostridium difficile correlates with tandem repeat sequence typing.
abstract: Subtyping of Clostridium difficile is crucial for outbreak investigations. An extended multilocus variable-number tandem-repeat analysis (eMLVA) of 14 variable number tandem repeat (VNTR) loci was validated in multiplex format compatible with a routine typing laboratory and showed excellent concordance with tandem repeat sequence typing (TRST) and high discriminatory power.
authors.affiliation: Department of Clinical Microbiology, Slagelse Hospital, Ingemannsvej 18, DK-4200 Slagelse, Denmark; Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark. Electronic address: mibf@regionsjaelland.dk.
authors.forename: Mie Birgitte Frid
authors.initials: MB
authors.lastname: Jensen
authors.affiliation: Department of Clinical Microbiology, Slagelse Hospital, Ingemannsvej 18, DK-4200 Slagelse, Denmark. Electronic address: jheg@regionsjaelland.dk.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Engberg
authors.affiliation: Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark. Electronic address: jlr@ssi.dk.
authors.forename: Jonas T
authors.initials: JT
authors.lastname: Larsson
authors.affiliation: Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark. Electronic address: keo@ssi.dk.
authors.forename: Katharina E P
authors.initials: KE
authors.lastname: Olsen
authors.affiliation: Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen, Denmark. Electronic address: mtd@ssi.dk.
authors.forename: Mia
authors.initials: M
authors.lastname: Torpdahl
mesh_terms: D016360:Clostridioides difficile / Q000145:classification* / Q000235:genetics*; D004269:DNA, Bacterial; D018598:Minisatellite Repeats; D058885:Multilocus Sequence Typing / Q000379:methods*; D010802:Phylogeny; D021521:Ribotyping / Q000379:methods*; D020080:Tandem Repeat Sequences
chemical_list: D004269:DNA, Bacterial
keywords: Clostridium difficile; Molecular subtyping; Multilocus variable-number tandem-repeat analysis; Tandem repeat sequence typing