title: Phage lytic proteins: biotechnological applications beyond clinical antimicrobials.
abstract: Most bacteriophages encode two types of cell wall lytic proteins: endolysins (lysins) and virion-associated peptidoglycan hydrolases. Both enzymes have the ability to degrade the peptidoglycan of Gram-positive bacteria resulting in cell lysis when they are applied externally. Bacteriophage lytic proteins have a demonstrated potential in treating animal models of infectious diseases. There has also been an increase in the study of these lytic proteins for their application in areas such as food safety, pathogen detection/diagnosis, surfaces disinfection, vaccine development and nanotechnology. This review summarizes the more recent developments, outlines the full potential of these proteins to develop new biotechnological tools and discusses the feasibility of these proposals.
authors.affiliation: a DairySafe Group, Department of Technology and Biotechnology of Dairy Products , Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Villaviciosa , Asturias , Spain and.
authors.forename: Lorena
authors.initials: L
authors.lastname: Rodríguez-Rubio
authors.affiliation: a DairySafe Group, Department of Technology and Biotechnology of Dairy Products , Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Villaviciosa , Asturias , Spain and.
authors.forename: Diana
authors.initials: D
authors.lastname: Gutiérrez
authors.affiliation: b Animal Biosciences and Biotechnology Laboratory , BARC, ARS, USDA , Beltsville , MD , USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Donovan
authors.affiliation: a DairySafe Group, Department of Technology and Biotechnology of Dairy Products , Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Villaviciosa , Asturias , Spain and.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Martínez
authors.affiliation: a DairySafe Group, Department of Technology and Biotechnology of Dairy Products , Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Villaviciosa , Asturias , Spain and.
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez
authors.affiliation: a DairySafe Group, Department of Technology and Biotechnology of Dairy Products , Instituto de Productos Lácteos de Asturias (IPLA-CSIC) , Villaviciosa , Asturias , Spain and.
authors.forename: Pilar
authors.initials: P
authors.lastname: García
mesh_terms: D000890:Anti-Infective Agents; D001435:Bacteriophages / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology; D010450:Endopeptidases; D059022:Food Safety; D009238:N-Acetylmuramoyl-L-alanine Amidase; D014764:Viral Proteins
chemical_list: D000890:Anti-Infective Agents; D014764:Viral Proteins; D010450:Endopeptidases; C001143:endolysin; D009238:N-Acetylmuramoyl-L-alanine Amidase
keywords: Biotechnology; disinfection; endolysins; food safety; nanotechnology; pathogen detection; vaccines; virion-associated peptidoglycan hydrolases