title: Developing genome-wide microsatellite markers of bamboo and their applications on molecular marker assisted taxonomy for accessions in the genus Phyllostachys.
abstract: Morphology-based taxonomy via exiguously reproductive organ has severely limitation on bamboo taxonomy, mainly owing to infrequent and unpredictable flowering events of bamboo. Here, we present the first genome-wide analysis and application of microsatellites based on the genome of moso bamboo (Phyllostachys edulis) to assist bamboo taxonomy. Of identified 127,593 microsatellite repeat-motifs, the primers of 1,451 microsatellites were designed and 1,098 markers were physically mapped on the genome of moso bamboo. A total of 917 markers were successfully validated in 9 accessions with ~39.8% polymorphic potential. Retrieved from validated microsatellite markers, 23 markers were selected for polymorphic analysis among 78 accessions and 64 alleles were detected with an average of 2.78 alleles per primers. The cluster result indicated the majority of the accessions were consistent with their current taxonomic classification, confirming the suitability and effectiveness of the developed microsatellite markers. The variations of microsatellite marker in different species were confirmed by sequencing and in silico comparative genome mapping were investigated. Lastly, a bamboo microsatellites database (http://www.bamboogdb.org/ssr) was implemented to browse and search large information of bamboo microsatellites. Consequently, our results of microsatellite marker development are valuable for assisting bamboo taxonomy and investigating genomic studies in bamboo and related grass species.
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Hansheng
authors.initials: H
authors.lastname: Zhao
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Peng
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Yue
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Lou
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Dong
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo and Rattan, Beijing 100102, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Gao
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping; D002965:Classification; D018745:Genome, Plant; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D031787:Sasa / Q000145:classification / Q000235:genetics*