title: Enhanced bio-decolorization of 1-amino-4-bromoanthraquinone-2-sulfonic acid by Sphingomonas xenophaga with nutrient amendment.
abstract: Bacterial decolorization of anthraquinone dye intermediates is a slow process under aerobic conditions. To speed up the process, in the present study, effects of various nutrients on 1-amino-4-bromoanthraquinone-2-sulfonic acid (ABAS) decolorization by Sphingomonas xenophaga QYY were investigated. The results showed that peptone, yeast extract and casamino acid amendments promoted ABAS bio-decolorization. In particular, the addition of peptone and casamino acids could improve the decolorization activity of strain QYY. Further experiments showed that l-proline had a more significant accelerating effect on ABAS decolorization compared with other amino acids. l-Proline not only supported cell growth, but also significantly increased the decolorization activity of strain QYY. Membrane proteins of strain QYY exhibited ABAS decolorization activities in the presence of l-proline or reduced nicotinamide adenine dinucleotide, while this behavior was not observed in the presence of other amino acids. Moreover, the positive correlation between l-proline concentration and the decolorization activity of membrane proteins was observed, indicating that l-proline plays an important role in ABAS decolorization. The above findings provide us not only a novel insight into bacterial ABAS decolorization, but also an l-proline-supplemented bioaugmentation strategy for enhancing ABAS bio-decolorization.
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: lvhonghj@163.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Xiaofan
authors.initials: X
authors.lastname: Guan
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China. Electronic address: jwangcn@sina.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Jiti
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China.
authors.forename: Haikun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism*; D013058:Mass Spectrometry; D020578:Sphingomonas / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: C527671:1-amino-4-bromoanthraquinone-2-sulfonic acid; D000880:Anthraquinones; D004396:Coloring Agents; D014874:Water Pollutants, Chemical
keywords: 1-Amino-4-bromoanthraquinone-2-sulfonic acid; Decolorization; Enhancement; Nutrients; Sphingomonas xenophaga