title: Genetic diversity in three bovine-like human G8P[14] and G10P[14] rotaviruses suggests independent interspecies transmission events.
abstract: The group A rotavirus (RVA) P[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. The present study describes the full-length genome analysis of two G8P[14] and one G10P[14] human RVAs detected in Italy. The strains possessed the typical bovine-like I2-R2-C2-M2-A3/A11-N2-T6-E2-H3 genotype constellation. All the segments of the two G8P[14] RVAs were most closely related to bovine(-like) strains but were relatively distant to each other, suggesting two independent interspecies transmission events. Likewise, the G10P[14] RVA gene segments were most similar to bovine(-like) RVAs but distinct from the G8 strains. The history of these strains probably involved the interspecies transmission of these viruses to humans from an as-yet-unidentified animal host, without evidence of reassortment events involving human RVAs. These results reinforce the potential of animal viruses to cross the host-species barrier, causing disease and increased viral genetic diversity in humans.
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Medici
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Tummolo
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Melisa Berenice
authors.initials: MB
authors.lastname: Bonica
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Laboratory for Clinical and Epidemiological Virology, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Heylen
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Laboratory for Clinical and Epidemiological Virology, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
authors.forename: Mark
authors.initials: M
authors.lastname: Zeller
authors.affiliation: Unit of Microbiology and Virology, Department of Clinical and Experimental Medicine, University of Parma, Parma, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Calderaro
authors.affiliation: KU Leuven - University of Leuven, Department of Microbiology and Immunology, Laboratory for Clinical and Epidemiological Virology, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
authors.forename: Jelle
authors.initials: J
authors.lastname: Matthijnssens
mesh_terms: D000818:Animals; D002675:Child, Preschool; D016000:Cluster Analysis; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D007223:Infant; D007558:Italy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000635:transmission* / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015047:Zoonoses / Q000635:transmission* / Q000821:virology
chemical_list: D012367:RNA, Viral