title: MiR-511 inhibits growth and metastasis of human hepatocellular carcinoma cells by targeting PIK3R3.
abstract: MicroRNAs (miRNAs) are critical for cancer development and progression. Elucidating the underlying mechanism of miRNAs in carcinogenesis may lead to novel diagnostic and therapeutic strategies for malignancy. In this study, we found that miR-511 expression was markedly downregulated in hepatocellular carcinoma (HCC) cell lines and tissues. Phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) was identified as a direct target of miR-511 and miR-511 expression inversely correlated with PIK3R3 mRNA expression in clinical HCC tissues. We further demonstrated that miR-511 suppressed HCC cell proliferation, migration, and invasion by repressing PIK3R3 expression. Investigations of possible mechanisms underlying these results revealed that miR-511 inhibited the phosphorylation of AKT and mTOR, which are key participants in the AKT/mTOR pathway. Taken together, our findings provide new insights into tumor suppression by miR-511 by negatively regulating the PIK3R3/AKT/mTOR signaling pathway.
authors.affiliation: Department of Radiology and Nuclear Medicine, Shanghai Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Cao
authors.forename: Weihua
authors.initials: W
authors.lastname: Dong
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Meng
authors.forename: Hongchao
authors.initials: H
authors.lastname: Liu
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Liao
authors.forename: Shiyuan
authors.initials: S
authors.lastname: Liu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000235:genetics; D019869:Phosphatidylinositol 3-Kinases / Q000096:biosynthesis*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics
chemical_list: D035683:MicroRNAs; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C578471:MIRN511 microRNA, human; C546842:MTOR protein, human; C523442:PIK3R3 protein, human