title: Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS.
abstract: A twoplex method using (12)C6 and (13)C6 stable isotope analogues (Δmass = 6 Da) of 2-aminobenzoic acid (2-AA) is described for quantitative analysis of N-glycans present on monoclonal antibodies and other glycoproteins using ultra performance liquid chromatography with sequential fluorescence and accurate mass tandem quadrupole time of flight (QToF) mass spectrometric detection.
authors.affiliation: Characterisation and Comparability Laboratory, NIBRT - The National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co., Dublin, Ireland. jonathan.bones@nibrt.ie.
authors.forename: Silvia
authors.initials: S
authors.lastname: Millán Martín
authors.forename: Cédric
authors.initials: C
authors.lastname: Delporte
authors.forename: Amy
authors.initials: A
authors.lastname: Farrell
authors.forename: Natalia
authors.initials: N
authors.lastname: Navas Iglesias
authors.forename: Niaobh
authors.initials: N
authors.lastname: McLoughlin
authors.forename: Jonathan
authors.initials: J
authors.lastname: Bones
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002250:Carbon Radioisotopes; D002853:Chromatography, Liquid / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D011134:Polysaccharides / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D062367:ortho-Aminobenzoates / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D002250:Carbon Radioisotopes; D006023:Glycoproteins; D011134:Polysaccharides; D062367:ortho-Aminobenzoates; C031385:anthranilic acid