title: Marine fish oils are not equivalent with respect to B-cell membrane organization and activation.
abstract: We previously reported that docosahexaenoic-acid (DHA)-enriched fish oil (DFO) feeding altered B-cell membrane organization and enhanced B-cell function. The purpose of this study was to evaluate whether menhaden oil (MO) and eicosapentaenoic-acid (EPA)-enriched fish oil (EFO) alters B-cell function/phenotype similarly. Mice were fed control (CON), MO, EFO or DFO diets for 5weeks. We evaluated the fatty acid composition of B-cell phospholipids, membrane microdomain organization, ex vivo B-cell functionality and in vivo B-cell subsets. Red blood cells and B cells were found to be strongly (r>0.85) and significantly (P<.001) correlated for major n-3 and n-6 long-chain polyunsaturated fatty acids (LCPUFAs). Compared to CON, MO and DFO resulted in decreased clustering of membrane microdomains, whereas EFO increased clustering. All fish oil treatments had 1.12-1.60 times higher CD40 expression following stimulation; however, we observed 0.86 times lower major histocompatibility complex class II expression and 0.7 times lower interleukin (IL)-6 production from EFO, but 3.25 times higher interferon-γ from MO and 1.5 times higher IL-6 from DFO. By 90min of incubation, MO had 1.11 times higher antigen uptake compared to CON, whereas EFO was 0.86 times lower. All fish oil treatments resulted in decreasingly mature splenic and bone marrow B-cell subsets. We conclude that diets high in n-3 LCPUFAs may elicit similar B-cell phenotypes but different organizational and functional outcomes. More specifically, these data suggest that the EPA and DHA content of a diet influences immunological outcomes, highlighting the importance of understanding how specific n-3 LCPUFAs modulate B-cell development and function.
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824.
authors.forename: Eric A
authors.initials: EA
authors.lastname: Gurzell
authors.affiliation: Department of Biochemistry & Molecular Biology, Department of Microbiology & Immunology, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834.
authors.forename: Heather
authors.initials: H
authors.lastname: Teague
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824.
authors.forename: David
authors.initials: D
authors.lastname: Duriancik
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Clinthorne
authors.affiliation: Department of Biochemistry & Molecular Biology, Department of Microbiology & Immunology, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834.
authors.forename: Mitchel
authors.initials: M
authors.lastname: Harris
authors.affiliation: Department of Biochemistry & Molecular Biology, Department of Microbiology & Immunology, East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834.
authors.forename: Saame Raza
authors.initials: SR
authors.lastname: Shaikh
authors.affiliation: Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824; College of Osteopathic Medicine, Michigan State University, East Lansing, MI 48824. Electronic address: imigjeni@msu.edu.
authors.forename: Jenifer I
authors.initials: JI
authors.lastname: Fenton
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D019013:CD40 Antigens / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects*; D004281:Docosahexaenoic Acids / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D015118:Eicosapentaenoic Acid / Q000737:chemistry*; D043371:Fatty Acids, Omega-6 / Q000737:chemistry; D005260:Female; D005395:Fish Oils / Q000737:chemistry*; D005399:Fishes; D005802:Genes, MHC Class II; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008297:Male; D021962:Membrane Microdomains; D051379:Mice; D018345:Mice, Knockout; D010743:Phospholipids / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism
chemical_list: D019013:CD40 Antigens; D043371:Fatty Acids, Omega-6; D005395:Fish Oils; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010743:Phospholipids; C033266:Menhaden oil; D004281:Docosahexaenoic Acids; D007371:Interferon-gamma; D015118:Eicosapentaenoic Acid
keywords: B cell; Fish oil; Immunomodulation; Inflammation; Membrane