title: Pregnancy serum facilitates hepatitis E virus replication in vitro.
abstract: Hepatitis E virus (HEV) infection causes high mortality in pregnant women. However, the pathogenic mechanisms of HEV infection in pregnant women remain unknown. In this study, the roles of pregnancy serum in HEV infection were investigated using an efficient cell culture system. HEV infection was exacerbated by supplementing with pregnancy serum, especially theat in third trimester of pregnancy. Oestrogen receptors (ER-α and ER-β) were activated in cells supplemented with pregnancy serum and were significantly inhibited during HEV infection. Type I IFN, especially IFN-β, showed delayed upregulation in HEV-infected cells supplemented with the serum in the third trimester of pregnancy, which indicated that delayed IFN-β expression may facilitate viral replication. Results suggested that pregnancy serum accelerated HEV replication by suppressing oestrogen receptors and type I IFN in the early stage of infection.
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Chenchen
authors.initials: C
authors.lastname: Yang
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, PR China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Yu
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Xianchen
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, 935 Jiaoling Road, Kunming, PR China.
authors.forename: Zhanlong
authors.initials: Z
authors.lastname: He
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Shenrong
authors.initials: S
authors.lastname: Jing
authors.affiliation: Kunming General Hospital of Chengdu Military Region, Kunming, PR China.
authors.forename: Huixuan
authors.initials: H
authors.lastname: Wang
authors.affiliation: Kunming General Hospital of Chengdu Military Region, Kunming, PR China.|Medical Faculty, Kunming University of Science and Technology, 727 Jingming Road, Kunming, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
mesh_terms: D000328:Adult; D002648:Child; D003470:Culture Media / Q000737:chemistry; D005260:Female; D016752:Hepatitis E virus / Q000502:physiology; D006801:Humans; D007370:Interferon Type I / Q000378:metabolism; D011247:Pregnancy; D011960:Receptors, Estrogen / Q000378:metabolism; D044967:Serum / Q000821:virology*; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D003470:Culture Media; D007370:Interferon Type I; D011960:Receptors, Estrogen