title: The phenanthroimidazole-based dizinc(II) complex as a fluorescent probe for the pyrophosphate ion as generated in polymerase chain reactions and pyrosequencing.
abstract: A highly selective and sensitive phenanthroimidazole tagged Mannich base type dizinc(II) fluorescent probe (R-Zn²⁺) has been developed for the pyrophosphate ion (PPi) with a very low limit of detection (LOD) of 0.25 ppm; this also assesses PPi from DNA polymerization chain reaction (PCR).
authors.affiliation: Centro de Química Estrutural, Complexo I, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal. bioinorg_anbu@yahoo.com pombeiro@tecnico.ulisboa.pt.
authors.forename: Sellamuthu
authors.initials: S
authors.lastname: Anbu
authors.forename: Subban
authors.initials: S
authors.lastname: Kamalraj
authors.forename: Anup
authors.initials: A
authors.lastname: Paul
authors.forename: Chelliah
authors.initials: C
authors.lastname: Jayabaskaran
authors.forename: Armando J L
authors.initials: AJ
authors.lastname: Pombeiro
mesh_terms: D004271:DNA, Fungal / Q000032:analysis; D011756:Diphosphates / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013098:Xylariales / Q000235:genetics; D015032:Zinc / Q000737:chemistry*
chemical_list: D004271:DNA, Fungal; D011756:Diphosphates; D005456:Fluorescent Dyes; D007093:Imidazoles; C107241:diphosphoric acid; D015032:Zinc