title: An ultrasensitive electrochemical DNA biosensor based on graphene/Au nanorod/polythionine for human papillomavirus DNA detection.
abstract: An ultrasensitive electrochemical DNA biosensor for human papillomavirus (HPV) detection was developed by electrochemical impedance spectroscopy and differential pulse voltammetry. A capture probe was immobilized on a glassy carbon electrode modified with graphene/Au nanorod/polythionine (G/Au NR/PT). Two auxiliary probes were designed and used to long-range self-assemble DNA nanostructure. The target DNA can connect DNA structure to the capture probe on the electrode surface. [Ru(phen)3](2+) was selected as a redox indicator for amplifying electrochemical signal significantly. Enhanced sensitivity was obtained through combining the excellent electric conductivity of G/Au NR/PT architecture and the long-range self-assembly DNA nanostructure with the multi-signal amplification. The DNA biosensor displayed excellent performance for HPV DNA detection over the range from 1.0×10(-13) to 1.0×10(-10) mol/L with a detection limit of 4.03×10(-14) mol/L. Furthermore, the proposed method can also be used for the detection of HPV DNA in human serum samples and provides a potential application of DNA detection in clinic research.
authors.affiliation: Shaanxi Academy of Environmental Science, Xi'an 710061, China. Electronic address: huayu.huang@hotmail.com.
authors.forename: Huayu
authors.initials: H
authors.lastname: Huang
authors.affiliation: Shaanxi Academy of Environmental Science, Xi'an 710061, China; School of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Wanqiao
authors.initials: W
authors.lastname: Bai
authors.affiliation: Department of Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Changxun
authors.initials: C
authors.lastname: Dong
authors.affiliation: School of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Guo
authors.affiliation: Shaanxi Academy of Environmental Science, Xi'an 710061, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D006801:Humans; D043942:Nanotubes / Q000737:chemistry*; D027383:Papillomaviridae / Q000302:isolation & purification* / Q000472:pathogenicity; D010640:Phenothiazines / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D010640:Phenothiazines; D006046:Gold; D006108:Graphite; C009469:thionine
keywords: Au nanorod; Electrochemical biosensor; Graphene; Human papillomavirus