title: [Tetrachlorodibenzo-p-dioxin-induced cleft palate because of partial loss of cell polarity to interfere with apoptosis during early developmental stage].
abstract: OBJECTIVE
  In this study, folic acid (FA) was tested for antiteratogenic effects on Tetrachlorodibenzo-p-dioxin (TCDD)-induced cleft palate in fetal mice.
  
  
  METHODS
  In the present study, pregnant mice were dosed with TCDD 24 µg/kg and with or without FA 5 mg/kg body weight on gestation day 10. Control group mice received sesame oil 50 ml/kg body weight on gestation day(GD)10. The mice were sacrificed on GD12.5, GD13.5, GD14.5, GD15.5 and GD16.5. From each pregnant mouse on GD16.5, embryos were obtained to examine under a dissecting microscope, and routine histology was performed for detection and classification of palatal clefts. The fetuses were prepared for histologic examination, scanning electron microscope and TdT-mediated X-dUTP nick and labeling (TUNEL). On GD12.5, GD13.5, GD14.5 and GD15.5. Meanwhile, real-time (RT)-PCR was employed to detect the mRNA expression levels about arylhydrocarbon receptor (AHR) and transforming growth factor (TGF)β3 in this animal model.
  
  
  RESULTS
  Total frequencies of clefts were 70.2% in TCDD group(group B) and 66.3% in TCDD+FA group(group C) in relation to control fetuses(group A). Filopodia disappeared completely at the medial edge epithelia surface on GD15.5 (group A), GD12.5 (group B) and GD14.5 (group C). the RT-PCR results showed that TGF -β3 expression was down-regulated on GD13.5 and GD14.5 compared to the control.
  
  
  CONCLUSIONS
  It is found that folic acid has no protects agaist 2.3.7.8-TCDD-indued cleft palate in the experiment. Meanwhile, TCDD repressed the TGF-β3 expression during the palatal development. Anormal apoptosis was induced by 2, 3, 7, 8-TCDD at the medial edge epithelia (MEE) during the early development stage.
authors.affiliation: Department of Cleft Lip and Palate Surgery, West China School of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Chenghao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Cleft Lip and Palate Surgery, West China School of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Cleft Lip and Palate Surgery, West China School of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Meng
authors.affiliation: Department of Cleft Lip and Palate Surgery, West China School of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Cleft Lip and Palate Surgery, West China School of Stomatology, State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China. Email: shibingcn@sina.com.
authors.forename: Bing
authors.initials: B
authors.lastname: Shi
mesh_terms: D000014:Abnormalities, Drug-Induced / Q000209:etiology* / Q000517:prevention & control; D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000032:analysis; D016764:Cell Polarity; D002972:Cleft Palate / Q000139:chemically induced* / Q000517:prevention & control; D004195:Disease Models, Animal; D005260:Female; D005333:Fetus; D005492:Folic Acid / Q000627:therapeutic use*; D051379:Mice; D008810:Mice, Inbred C57BL; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000032:analysis / Q000235:genetics; D013723:Teratogens / Q000633:toxicity*; D053782:Transforming Growth Factor beta3 / Q000032:analysis* / Q000235:genetics
chemical_list: D015415:Biomarkers; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D018336:Receptors, Aryl Hydrocarbon; D013723:Teratogens; D053782:Transforming Growth Factor beta3; D005492:Folic Acid