title: Differentially expressed genes in the fat body of Bombyx mori in response to phoxim insecticide.
abstract: The silkworm, Bombyx mori, is an economically important insect. However, poisoning of silkworms by organophosphate pesticides causes tremendous loss to the sericulture. The fat body is the major tissue involved in detoxification and produces antimicrobial peptides and regulates hormones. In this study, a microarray system comprising 22,987 oligonucluotide 70-mer probes was employed to examine differentially expressed genes in the fat body of B. mori exposed to phoxim insecticide. The results showed that a total of 774 genes were differentially expressed upon phoxim exposure, including 500 up-regulated genes and 274 down-regulated genes. The expression levels of eight detoxification-related genes were up-regulated upon phoxim exposure, including six cytochrome P450s and two glutathione-S-transferases. It was firstly found that eight antimicrobial peptide genes were down-regulated, which might provide important references for studying the larvae of B. mori become more susceptible to microbial infections after phoxim treatment. In addition, we firstly detected the expression level of metamorphosis-related genes after phoxim exposure, which may lead to impacted reproduction. Our results may facilitate the overall understanding of the molecular mechanism of multiple pathways following exposure to phoxim insecticide in the fat body of B. mori.
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: F C
authors.initials: FC
authors.lastname: Li
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: B B
authors.initials: BB
authors.lastname: Wang
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: K Z
authors.initials: KZ
authors.lastname: Xu
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: M
authors.initials: M
authors.lastname: Ni
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: W D
authors.initials: WD
authors.lastname: Shen
authors.affiliation: School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, China. Electronic address: lib@suda.edu.cn.
authors.forename: B
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics*; D005216:Fat Body / Q000187:drug effects* / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D017344:Genes, Insect / Q000187:drug effects*; D007306:Insecticides / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*
chemical_list: D007306:Insecticides; D009946:Organothiophosphorus Compounds; C003135:phoxim
keywords: Fat body; Gene expression profiles; Phoxim; Silkworm