title: High content screening for modulators of cardiac differentiation in human pluripotent stem cells.
abstract: Chemical genomics has the unique potential to expose novel mechanisms of complex cellular biology through screening of small molecules in in vitro assays of a biological phenotype of interest, followed by target identification. In the case of disease-specific assays, the cellular proteins identified might constitute novel drug targets, and the small molecules themselves might be developed as drug leads. In cardiovascular biology, a chemical genomics approach to study the formation of cardiomyocyte, vascular endothelial, and smooth muscle lineages might contribute to therapeutic regeneration. Here, we describe methods used to develop high content screening assays implementing multipotent cardiovascular progenitors derived from human pluripotent stem cells and have identified novel compounds that direct cardiac differentiation.
authors.affiliation: Muscle Development and Regeneration Program, Sanford-Burnham Medical Research Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA.
authors.forename: Sean
authors.initials: S
authors.lastname: Spiering
authors.forename: Herman
authors.initials: H
authors.lastname: Davidovics
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Bushway
authors.forename: Mark
authors.initials: M
authors.lastname: Mercola
authors.forename: Erik
authors.initials: E
authors.lastname: Willems
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000187:drug effects*; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D020796:Receptor, Platelet-Derived Growth Factor alpha; C000589015:MESP1 protein, human