title: Salvianolic Acid B Protects Normal Human Dermal Fibroblasts Against Ultraviolet B Irradiation-Induced Photoaging Through Mitogen-Activated Protein Kinase and Activator Protein-1 Pathways.
abstract: Exposure to ultraviolet (UV) light causes increased matrix metalloproteinase (MMP) activity and decreased collagen synthesis, leading to skin photoaging. Salvianolic acid B (SAB), a polyphenol, was extracted and purified from salvia miltiorrhiza. We assessed effects of SAB on UVB-induced photoaging and investigated its molecular mechanism of action in UVB-irradiated normal human dermal fibroblasts. Our results show that SAB significantly inhibited the UVB-induced expression of metalloproteinases-1 (MMP-1) and interleukin-6 (IL-6) while promoting the production of type I procollagen and transforming growth factor β1 (TGF-β1). Moreover, treatment with SAB in the range of 1-100 μg/mL significantly inhibited UVB-induced extracellular signal-regulated kinase (ERK), Jun N-terminal kinase (JNK) and p38 phosphorylation, which resulted in decreasing UVB-induced phosphorylation of c-Fos and c-Jun. These results indicate that SAB downregulates UV-induced MMP-1 expression by inhibiting Mitogen-activated protein kinase (MAPK) signaling pathways and activator protein-1 (AP-1) activation. Our results suggest a potential use for SAB in skin photoprotection.
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, Yongin-si, Gyeonggi-do, Korea.|College of Biotechnology, Dalian Polytechnic University, Ganjingzi-Qu, Dalian, China.
authors.forename: Zhengwang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, Yongin-si, Gyeonggi-do, Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, Yongin-si, Gyeonggi-do, Korea.
authors.forename: Eunson
authors.initials: E
authors.lastname: Hwang
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, Yongin-si, Gyeonggi-do, Korea.
authors.forename: Mengyang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Biotechnology, Dalian Polytechnic University, Ganjingzi-Qu, Dalian, China.
authors.forename: Fengxie
authors.initials: F
authors.lastname: Jin
authors.affiliation: College of Basic Medicine, Beijing University of Chinese Medicine, Chaoyang-Qu, Beijing, China.
authors.forename: Baochun
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, Yongin-si, Gyeonggi-do, Korea.
authors.forename: Tae Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D001572:Benzofurans / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D053630:Depsides
chemical_list: D001572:Benzofurans; D020928:Mitogen-Activated Protein Kinases; D018808:Transcription Factor AP-1; C076944:salvianolic acid B; D053630:Depsides