title: C-terminus of Sororin interacts with SA2 and regulates sister chromatid cohesion.
abstract: Sororin is a conserved protein required for accurate separation of sister chromatids in each cell cycle. Sororin is recruited to chromatin during DNA replication, protects sister chromatid cohesion in S and G2 phase, and regulates the resolution of sister chromatid cohesion in mitosis. Sororin binds to cohesin complex, but how Sororin and cohesin subunits interact remains unclear. Here we report that the C-terminus of Sororin, especially the last 12 amino acid (aa) residues, is important for Sororin to bind cohesin core subunit SA2. Deletion of the last 12aa residues not only inhibits the interactions between Sororin and SA2 but also causes precocious chromosome separation. Our data suggest that the C-terminus of Sororin functions as an anchor binding to SA2, which facilitates other conserved motifs on Sororin to interact with other proteins to regulate sister chromatid cohesion and separation.
authors.affiliation: a Texas Children's Cancer Center ; Department of Pediatric Hematology/Oncology ; Baylor College of Medicine ; Houston , TX USA.
authors.forename: Nenggang
authors.initials: N
authors.lastname: Zhang
authors.forename: Debananda
authors.initials: D
authors.lastname: Pati
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D002842:Chromatids / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D020090:Chromosome Segregation; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D021122:Protein Subunits / Q000378:metabolism*; D017384:Sequence Deletion; D013329:Structure-Activity Relationship; D000097722:Cohesins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D021122:Protein Subunits; D000097722:Cohesins; C500695:CDCA5 protein, human
keywords: SA2; Sororin; cell cycle; cohesin; sister chromatid cohesion