title: Metagenomic insights into the effects of fructo-oligosaccharides (FOS) on the composition of fecal microbiota in mice.
abstract: Fructo-oligosaccharides (FOS) are usually regarded as a type of prebiotic, favorably stimulating the growth of bifidobacteria and lactobacilli. However, they are not the specific substrates for these target species, and other bacteria, such as Streptococcus, Escherichia, and Clostridium, have been shown to be able to utilize FOS. Previous studies have mainly investigated only a few bacteria groups, and few reports analyzed the global effects of FOS on intestinal microbial communities. In this study the effects of FOS on gut bacteria in mice were investigated through a 16S rRNA metagenomic analysis. In the FOS-low group, the abundance of Actinobacteria significantly increased and that of Bacteroidetes decreased after FOS diet (5%) for 3 weeks. In the FOS-high group, Enterococcus was promoted and levels of Bifidobacterium and Olsenella both notably increased after FOS diet (25%) and the microbiota tended to revert to initial structure 2 weeks after FOS treatment ceased. The most striking observation was that Olsenella became a dominant genus comparable with Bifidobacterium after FOS treatment, and one strain of Olsenella, isolated from mice feces, was confirmed, for the first time, to be capable of using FOS. The results indicated that metagenomic analysis was helpful to reveal the FOS effects on the global composition of gut communities and new target for future studies.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University , Wuxi 214122, People's Republic of China.
authors.forename: Bingyong
authors.initials: B
authors.lastname: Mao
authors.forename: Dongyao
authors.initials: D
authors.lastname: Li
authors.forename: Jianxin
authors.initials: J
authors.lastname: Zhao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Liu
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Gu
authors.forename: Yong Q
authors.initials: YQ
authors.lastname: Chen
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D039903:Actinobacteria / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001644:Bifidobacterium / Q000187:drug effects / Q000254:growth & development; D004269:DNA, Bacterial / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008297:Male; D056186:Metagenomics; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000187:drug effects*; D009844:Oligosaccharides / Q000494:pharmacology*; D056692:Prebiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D009844:Oligosaccharides; D056692:Prebiotics; D012336:RNA, Ribosomal, 16S; C116580:fructooligosaccharide
keywords: 16S rRNA metagenomic; Olsenella; fructo-oligosaccharides; intestinal microbiota; prebiotic