title: Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival.
abstract: OBJECTIVE
  To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC).
  
  
  METHODS
  A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods.
  
  
  RESULTS
  The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.
  
  
  CONCLUSIONS
  The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.
authors.affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.
authors.forename: Dmitriy Sergeevich
authors.initials: DS
authors.lastname: Kobyakov
authors.affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.
authors.forename: Ashot Merudzhanovich
authors.initials: AM
authors.lastname: Avdalyan
authors.affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.
authors.forename: Aleksandr Fedorovich
authors.initials: AF
authors.lastname: Lazarev
authors.affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.
authors.forename: Elena Leonidovna
authors.initials: EL
authors.lastname: Lushnikova
authors.affiliation: 1 Budget Institution, Kogalym City Hospital, Kogalym 628484, Russia ; 2 Laboratory of Molecular Diagnostics of Altai Branch of Russian N.N. Blokhin Cancer Research Center, Barnaul 656049, Russia ; 3 Federal State Budget Institution Research Institute of Regional Pathology and Pathomorphology of Siberian Branch of Russian Academy of Medical Sciences, Novosibirsk 630117, Russia.
authors.forename: Lev Moiseevich
authors.initials: LM
authors.lastname: Nepomnyashchikh
keywords: Argyrophilic nucleolar organizer region (AgNOR); MIB-1; non-small cell lung cancer (NSCLC); survival