title: Effect of volatile oil from Blumea Balsamifera (L.) DC. leaves on wound healing in mice.
abstract: OBJECTIVE
  To assess the effectiveness of violate oil from Blumea Balsamifera (L.) DC. leaves (BB oil) on wound healing in mice.
  
  
  METHODS
  Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield BB oil1/5 and BB oil1/10 were applied to the wounded skin before wound healing conditions were assessed by healing rate, histopathology, and contents of collagen, hydroxyproline, and Neuropeptide Substance P (SP). All above results were compared with the efficacies of the control, pure olive oil, basic fibroblast growth factor (BFGF), and cream of Jing Wan Hong (JWH).
  
  
  RESULTS
  BB oil1/5 and BB oil1/10 improved wound contraction and closure. Histopathology study further confirmed a desirable histological organization of wound tissues. BB oil1/5 and BB oil1/10 reduced the number of inflammatory cells, increased wound-healing rates, and significantly increased the hydroxyproline content. Both BB oil1/5 and BB oil1/10 improved formation of collagen, and reduced the frequency of fibroblasts. Moreover, BB oil1/5 and BB oil1/10 markedly promoted SP expression. However, undiluted BB oil may induce skin thickening and hardening, inhibite collagen synthesis and delay complete skin wound healing.
  
  
  CONCLUSIONS
  The BB oil1/5 and BB oil1/10 promoted capillary regeneration, blood circulation, collagen deposition, granular tissue formation, epithelial deposition, and wound contraction. The mechanism underlying the action might be related to induction of SP secretion, and the proliferation and differentiation of mesenchymal cells.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Pang
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Wanjin
authors.initials: W
authors.lastname: Fu
authors.forename: Zuowang
authors.initials: Z
authors.lastname: Fan
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Chen
authors.forename: Fulai
authors.initials: F
authors.lastname: Yu
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D009822:Oils, Volatile / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology*
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D010938:Plant Oils; D003094:Collagen