title: Transgene expression and differentiation of baculovirus-transduced adipose-derived stem cells from dystrophin-utrophin double knock-out mouse.
abstract: In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China ; Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Qiuling
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Qiongxiang
authors.initials: Q
authors.lastname: Zhai
authors.affiliation: Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Geng
authors.affiliation: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510085, Guangdong Province, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Kong
authors.affiliation: Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
keywords: Duchenne muscular dystrophy; adipose-derived stem cells; baculovirus; gene therapy; microdystrophin; myogenesis; neural regeneration; β-catenin