title: The effect of sex on humanin levels in healthy adults and patients with uncomplicated type 1 diabetes mellitus.
abstract: Diabetes mellitus (DM) is associated with a loss of renal and vascular protection in women compared with men, but the responsible mechanisms are unclear. Recent experimental work implicated humanin (HN) as a novel cytoprotective hormone in DM. Our goal was to measure sex-related differences in HN levels in uncomplicated type 1 DM patients (T1D) and healthy controls (HC), as well as the interaction between HN, circulating neurohormones, and vascular function. Plasma HN, cGMP and aldosterone, blood pressure (BP), glomerular filtration rate, and effective renal plasma flow (inulin and para-aminohippurate) were measured in HC (11 men, 10 women) and T1D (23 men and 18 women) during clamped euglycemia (4-6 mmol·L(-1)). Plasma HN levels were generally lower in HC men by comparison with the women, but the differences were not statistically significant. In contrast, levels in the T1D men were higher compared with the T1D women (p = 0.026) and HC men (p < 0.0001). In the HC men, but not the women, HN correlated negatively with BP, but not with renal function, cGMP, or aldosterone. In the T1D men, HN negatively correlated with plasma cGMP. In the T1D women, HN did not correlate with neurohormones or vascular function. Future work should determine the role of HN in the pathogenesis of sex-related vascular function differences in DM.
authors.affiliation: Division of Nephrology, University Health Network, University of Toronto, Toronto General Hospital, 585 University Avenue, Toronto, ON M5G 2N2, Canada., Department of Pharmacology and Toxicology, Faculty of Medicine University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada.
authors.forename: Yuliya
authors.initials: Y
authors.lastname: Lytvyn
authors.forename: Junxiang
authors.initials: J
authors.lastname: Wan
authors.forename: Vesta
authors.initials: V
authors.lastname: Lai
authors.forename: Pinchas
authors.initials: P
authors.lastname: Cohen
authors.forename: David Z I
authors.initials: DZ
authors.lastname: Cherney
mesh_terms: D000328:Adult; D000450:Aldosterone / Q000097:blood; D001794:Blood Pressure; D015331:Cohort Studies; D006152:Cyclic GMP / Q000097:blood; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000503:physiopathology; D004730:Endothelium, Vascular / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005919:Glomerular Filtration Rate; D015309:Glucose Clamp Technique; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood*; D008297:Male; D012189:Retrospective Studies; D012727:Sex Characteristics; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D000450:Aldosterone; D006152:Cyclic GMP; D006442:Glycated Hemoglobin; D047908:Intracellular Signaling Peptides and Proteins; C517652:hemoglobin A1c protein, human; C427211:humanin
keywords: GMPc; aldosterone; aldostérone; blood pressure; cGMP; débit plasmatique rénal effectif; effective renal plasma flow; glomerular filtration rate; pression sanguine; taux de filtration glomérulaire