title: Acupuncture activates signal transduction pathways related to brain-tissue restoration after ischemic injury.
abstract: A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method. Rats were treated with acupuncture at either Dazhui (DU14), Renzhong (DU26), Baihui (DU20), or a non-meridian point. Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture. Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group, while only two were activated in the acupuncture- control group. In contrast, analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group, whereas four pathways were activated in the acupuncture-control group. Furthermore, the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group, but unlike the acupuncture-control group, the majority of activated proteins were key proteins in the signaling pathways. Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury, and that this is based on a combination of effects resulting from multiple pathways, targets, and means.
authors.affiliation: Key Laboratory of Meridians and Viscera, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
authors.forename: Haomei
authors.initials: H
authors.lastname: Tian
authors.affiliation: Key Laboratory of Meridians and Viscera, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou 545001, Guangxi Zhuang Autonomous Region, China.
authors.forename: Junbao
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Ningxiang Hospital of Traditional Chinese Medicine, Ningxiang 410600, Hunan Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Postgraduate College, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
authors.forename: Hening
authors.initials: H
authors.lastname: Cai
authors.affiliation: Postgraduate College, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Laboratory of Acupuncture Bioinformatics, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.
authors.forename: Chutao
authors.initials: C
authors.lastname: Chen
keywords: Baihui; Dazhui; Renzhong; acupuncture; brain injury; cerebral ischemia; ischemic injury; nerve injury; neural regeneration; protein-chip technology; signal-transduction pathways