title: B7-H1 and B7-H3 are independent predictors of poor prognosis in patients with non-small cell lung cancer.
abstract: B7-H1 and B7-H3, two members of the B7 family that are thought to regulate T-cell activation, are expressed in human non-small cell lung cancer (NSCLC). However, their prognostic significance is poorly understood. In the present study we reported that B7-H1 and B7-H3 were expressed in 96/128 (72.7%) and 89/128 (69.5%) samples, respectively. B7-H1 and B7-H3 expression and the number of infiltrating T-cell intracellular antigen-1+ and interferon-γ+ cells in NSCLC tissues were significantly higher than those in the adjacent tissues (p<0.01). High B7-H1 or B7-H3 expression was associated with lymph node metastasis and TNM stage (p<0.05, respectively). Sex, TNM stage, B7-H1, B7-H3, and T-cell intracellular antigen-1 expression remained significant prognostic factors after adjusting for other prognostic factors in a multivariate Cox proportional hazards regression model. In vitro studies revealed that knockdown of B7-H3 on tumor cells enhanced T-cell growth and interferon-γ secretion when stimulated by anti-CD3 and anti-CD28 monoclonal antibodies. Interferon-γ reduced CXCR4 expression on cancer cells and inhibited the CXCL12-induced cell migration.B7-H1 and B7-H3 are independent predictors of poorer survival in patients with NSCLC. Interference of the signal pathways of these negative regulatory molecules might be a new strategy for treating NSCLC.
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Pathology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Pathology, Punan Hospital, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yao
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Duan
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Rongrui
authors.initials: R
authors.lastname: Liang
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Soochow University, Suzhou, China.|Jiangsu Institute of Clinical Immunology, Suzhou, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tao
mesh_terms: D060887:B7 Antigens / Q000235:genetics / Q000378:metabolism*; D060890:B7-H1 Antigen / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000276:immunology / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002633:Chemotaxis; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008207:Lymphatic Metastasis; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D016016:Proportional Hazards Models; D019718:Receptors, CXCR4 / Q000378:metabolism; D012307:Risk Factors; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014162:Transfection; D015854:Up-Regulation
chemical_list: D060887:B7 Antigens; D060890:B7-H1 Antigen; D014408:Biomarkers, Tumor; C423236:CD274 protein, human; C423237:CD276 protein, human; C527276:CXCR4 protein, human; C573415:IFNG protein, human; D019718:Receptors, CXCR4; D007371:Interferon-gamma