title: Ripple structure-generated hybrid electrokinetics for on-chip mixing and separating of functionalized beads.
abstract: We present an electrokinetics-based microfluidic platform that is capable of on-chip manipulating, mixing, and separating microparticles through adjusting the interrelated magnitudes of dielectrophoresis and AC electroosmosis. Hybrid electrokinetic phenomenon is generated from an electric field-induced micro-ripple structure made of ultraviolet-curable glue. Size-dependent particle separation and selective removal over the ripple structure is demonstrated successfully. Varying the waveform from sine-wave to square-wave allows generating a fluid convection at specific positions to mix the antibody-functionalized beads and antigen. Potential application in the bead-based immunoassay was also demonstrated for immuno-reaction and subsequently separating the bead-bead aggregate and non-binding beads on-chip.
authors.affiliation: National Nano Device Laboratories, National Applied Research Laboratories , Tainan, Taiwan.
authors.forename: I-Fang
authors.initials: IF
authors.lastname: Cheng
authors.affiliation: Department of Biomedical Engineering, National Cheng Kung University , Tainan, Taiwan.
authors.forename: Sheng-Chuan
authors.identifier: 0000-0001-8645-3534
authors.initials: SC
authors.lastname: Chiang
authors.affiliation: Institute of Nanotechnology and Microsystems Engineering, National Cheng Kung University , Tainan, Taiwan.
authors.forename: Cheng-Che
authors.initials: CC
authors.lastname: Chung
authors.affiliation: Medical Laboratory Science and Biotechnology, National Cheng Kung University , Tainan, Taiwan.
authors.forename: Trai-Ming
authors.initials: TM
authors.lastname: Yeh
authors.forename: Hsien-Chang
authors.initials: HC
authors.lastname: Chang