title: Magnetic molecularly imprinted polymer for the selective extraction of quercetagetin from Calendula officinalis extract.
abstract: A new magnetic molecularly imprinted polymers (MMIPs) for quercetagetin was prepared by surface molecular imprinting method using super paramagnetic core-shell nanoparticle as the supporter. Acrylamide as the functional monomer, ethyleneglycol dimethacrylate as the crosslinker and acetonitrile as the porogen were applied in the preparation process. Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and Vibrating sample magnetometer (VSM) were applied to characterize the MMIPs, and High performance liquid chromatography (HPLC) was utilized to analyze the target analytes. The selectivity of quercetagetin MMIPs was evaluated according to their recognition to template and its analogues. Excellent binding for quercetagetin was observed in MMIPs adsorption experiment, and the adsorption isotherm models analysis showed that the homogeneous binding sites were distributed on the surface of the MMIPs. The MMIPs were employed as adsorbents in solid phase extraction for the determination of quercetagetin in Calendula officinalis extracts. Furthermore, this method is fast, simple and could fulfill the determination and extraction of quercetagetin from herbal extract.
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
authors.forename: Run-Tian
authors.initials: RT
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemistry of Northwestern Plant Resources of CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China. Electronic address: shiyp@licp.cas.cn.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Shi
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D020106:Acrylamide / Q000737:chemistry; D000327:Adsorption; D020838:Calendula; D002867:Chromones / Q000032:analysis* / Q000737:chemistry; D047309:Flavones; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D010936:Plant Extracts / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000097:Acetonitriles; D002867:Chromones; D047309:Flavones; D058185:Magnetite Nanoparticles; D008689:Methacrylates; D010936:Plant Extracts; D011108:Polymers; D020106:Acrylamide; D012822:Silicon Dioxide; C004919:ethylene dimethacrylate; C079163:quercetagetin; C032159:acetonitrile
keywords: Magnetic molecularly imprinted polymers; Quercetagetin; Solid phase extraction; Surface imprinting method; Traditional Chinese medicine