title: Potent anti-proliferative effects against oral and cervical cancers of Thai medicinal plants selected from the Thai/Lanna medicinal plant recipe database "MANOSROI III".
abstract: BACKGROUND
  Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including oral and cervical cancers.
  
  
  OBJECTIVE
  To investigate anti-proliferative activity on human cervical (HeLa) and oral (KB) cancer cell lines of medicinal plants selected from Thai/Lanna medicinal plant recipe database "MANOSROI III".
  
  
  METHODS
  Twenty-three methanolic plant crude extracts were tested for phytochemicals and anti-proliferative activity on HeLa and KB cell lines for 24 h by the sulforhodamine B (SRB) assay at the doses of 1 × 10(1)-1 × 10(-6 )mg/ml. The nine extracts with the concentrations giving 50% growth inhibition (GI50) lower than 100 µg/ml were further semi-purified by liquid/liquid partition in order to evaluate and enhance the anti-proliferative potency.
  
  
  RESULTS
  All extracts contained steroids/triterpenoids, but not xanthones. The methanolic extracts of Gloriosa superba L. (Colchinaceae) root and Albizia chinensis (Osbeck) Merr. (Leguminosae-Mimosoideae) wood gave the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.91 (6.0- and 0.31-fold of cisplatin and doxorubicin) and 0.16 µg/ml (28.78- and 82.29-fold of cisplatin and doxorubicin), respectively. Hexane and methanol-water fractions of G. superba exhibited the highest anti-proliferative activity on HeLa and KB cell lines with the GI50 values of 0.15 (37- and 1.9-fold of cisplatin and doxorubicin) and 0.058 µg/ml (77.45- and 221.46-fold of cisplatin and doxorubicin), respectively.
  
  
  CONCLUSIONS
  This study has demonstrated the potential of plants selected from MANOSROI III database especially G. superba and A. chinensis for further development as anti-oral and cervical cancer agents.
authors.affiliation: Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand .
authors.forename: Aranya
authors.initials: A
authors.lastname: Manosroi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Akazawa
authors.forename: Kassara
authors.initials: K
authors.lastname: Pattamapun
authors.forename: Worapong
authors.initials: W
authors.lastname: Kitdamrongtham
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akihisa
authors.forename: Worapaka
authors.initials: W
authors.lastname: Manosroi
authors.forename: Jiradej
authors.initials: J
authors.lastname: Manosroi
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D049109:Cell Proliferation / Q000187:drug effects*; D016208:Databases, Factual; D005260:Female; D006367:HeLa Cells; D006801:Humans; D007624:KB Cells; D009062:Mouth Neoplasms / Q000188:drug therapy / Q000473:pathology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal; D013785:Thailand; D016896:Treatment Outcome; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts
keywords: Anti-proliferative effects; cervical cancer; oral cancer; thai medicinal plants; “MANOSROI III” database