title: The three-dimensional structure of the cellobiohydrolase Cel7A from Aspergillus fumigatus at 1.5 Å resolution.
abstract: The enzymatic degradation of plant cell-wall cellulose is central to many industrial processes, including second-generation biofuel production. Key players in this deconstruction are the fungal cellobiohydrolases (CBHs), notably those from family GH7 of the carbohydrate-active enzymes (CAZY) database, which are generally known as CBHI enzymes. Here, three-dimensional structures are reported of the Aspergillus fumigatus CBHI Cel7A solved in uncomplexed and disaccharide-bound forms at resolutions of 1.8 and 1.5 Å, respectively. The product complex with a disaccharide in the +1 and +2 subsites adds to the growing three-dimensional insight into this family of industrially relevant biocatalysts.
authors.affiliation: Department of Chemistry, University of York, York Structural Biology Laboratory, York YO10 5DD, England.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Moroz
authors.affiliation: Novozymes Inc., 1443 Drew Avenue, Davis, CA 95618, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Maranta
authors.affiliation: Novozymes Inc., 1443 Drew Avenue, Davis, CA 95618, USA.
authors.forename: Tarana
authors.initials: T
authors.lastname: Shaghasi
authors.affiliation: Novozymes Inc., 1443 Drew Avenue, Davis, CA 95618, USA.
authors.forename: Paul V
authors.initials: PV
authors.lastname: Harris
authors.affiliation: Department of Chemistry, University of York, York Structural Biology Laboratory, York YO10 5DD, England.
authors.forename: Keith S
authors.initials: KS
authors.lastname: Wilson
authors.affiliation: Department of Chemistry, University of York, York Structural Biology Laboratory, York YO10 5DD, England.
authors.forename: Gideon J
authors.initials: GJ
authors.lastname: Davies
mesh_terms: D001232:Aspergillus fumigatus / Q000201:enzymology*; D020134:Catalytic Domain; D002475:Cellobiose / Q000737:chemistry; D002482:Cellulose / Q000737:chemistry; D043366:Cellulose 1,4-beta-Cellobiosidase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry*; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary
chemical_list: D005656:Fungal Proteins; D002475:Cellobiose; D002482:Cellulose; D043366:Cellulose 1,4-beta-Cellobiosidase
keywords: biofuels; carbohydrate-active enzyme; cellulase; thermal stability