title: Synthesis of silver nanoparticles using Solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line MCF 7.
abstract: In the present study, we have synthesized silver nanoparticles by a simple and eco-friendly method using unripe fruits of Solanum trilobatum. The aqueous silver ions when exposed to unripe fruits extract were reduced and stabilized over long time resulting in biosynthesis of surface functionalized silver nanoparticles. The bio-reduced silver nanoparticles were characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX) and X-ray diffraction (XRD). These biologically synthesized silver nanoparticles were tested for its antibacterial activity against few human pathogenic bacteria including Gram-positive (Streptococcus mutans, Enterococcus faecalis) and Gram-negative (Escherichia coli, Klebsiella pneumoniae) bacteria. In addition, we also demonstrated anticancer activity of these nanoparticles in vitro against human breast cancer cell line (MCF 7) using MTT, nuclear morphology assay, Western blot and RT-PCR expression. These results taken together show the potential applications of biosynthesized silver nanoparticles using S. trilobatum fruits.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: manikandanramar@yahoo.co.in.
authors.forename: Manikandan
authors.initials: M
authors.lastname: Ramar
authors.affiliation: Department of Biochemistry, Annai Veilankanni's College for Women, Chennai 600 015, India.
authors.forename: Beulaja
authors.initials: B
authors.lastname: Manikandan
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Prabhu Narayanan
authors.initials: PN
authors.lastname: Marimuthu
authors.affiliation: Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, India.
authors.forename: Thiagarajan
authors.initials: T
authors.lastname: Raman
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Anjugam
authors.initials: A
authors.lastname: Mahalingam
authors.affiliation: Department of Animal Health and Management, Alagappa University, Karaikudi 630 003, India.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Subramanian
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Karthick
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India. Electronic address: marumugam7@hotmail.com.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Munusamy
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001940:Breast / Q000187:drug effects / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005638:Fruit / Q000737:chemistry; D055772:Green Chemistry Technology; D006801:Humans; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000627:therapeutic use; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D032322:Solanum / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D012834:Silver
keywords: Antibacterial activity; Anticancer; Biological synthesis