title: A rapid and sensitive LC-MS/MS method for quantification of 3,29-dibenzoyl rarounitriol in rat plasma: application to a pharmacokinetic study.
abstract: A rapid, sensitive and high-throughput liquid chromatography-tandem mass spectrometry was established and validated to assay the concentrations of 3,29-dibenzoyl rarounitriol in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate and separated on a Hypersil Gold C18 column (50 × 4.6 mm, 3 µm) at an isocratic flow rate of 0.5 mL/min using methanol-10 mm ammonium acetate-formic acid (90:10:0.1, v/v/v) as mobile phase. The total run time was 5 min for each sample. MS/MS detection was accomplished in selected reaction monitoring mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.125-50 ng/mL with lower limit of quantification of 0.125 ng/mL. The intra- and inter-day precisions were <10.1% in terms of coefficient of variation, and the accuracy was within ±11.7% in terms of relative error. The developed method was successfully applied to a pharmacokinetic study of 3,29-dibenzoyl rarounitriol following intragastric administration of 3.65 mg/kg to Wistar rats.
authors.affiliation: Gastrointestinal Medicine, the First Hospital of Jilin University, Changchun, 130021, China.|Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Emergency Department, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
authors.forename: Youxin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
authors.forename: Naiqiang
authors.initials: N
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, 067000, China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
authors.forename: Changjian
authors.initials: C
authors.lastname: Liu
authors.affiliation: Gastrointestinal Medicine, the First Hospital of Jilin University, Changchun, 130021, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Meng
mesh_terms: D000818:Animals; D001565:Benzoates / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D059625:Liquid-Liquid Extraction / Q000379:methods; D008297:Male; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*; D029778:Trichosanthes / Q000737:chemistry; D014315:Triterpenes / Q000097:blood* / Q000737:chemistry
chemical_list: C000604165:3,29-dibenzoyl rarounitriol; D001565:Benzoates; D014315:Triterpenes
keywords: 3,29-dibenzoyl rarounitriol; Trichosanthes kirilowii; multiflorane triterpene esters; pharmacokinetics; rat plasma