title: Bacillus dabaoshanensis sp. nov., a Cr(VI)-tolerant bacterium isolated from heavy-metal-contaminated soil.
abstract: A Cr(VI)-tolerant, Gram-staining-positive, rod-shaped, endospore-forming and facultative anaerobic bacterium, designated as GSS04(T), was isolated from a heavy-metal-contaminated soil. Strain GSS04(T) was Cr(VI)-tolerant with a minimum inhibitory concentration of 600 mg l(-1) and was capable of reducing Cr(VI) under both aerobic and anaerobic conditions. Growth occurred with presence of 0-3 % (w/v) NaCl (optimum 1 %), at pH 5.5-10.0 (optimum pH 7.0) and 15-50 °C (optimum 30-37 °C). The main respiratory quinone was MK-7 and the major fatty acids were anteiso-C15:0 and iso-C15:0. The DNA G+C content was 41.1 mol%. The predominant polar lipid was diphosphatidylglycerol. Based on 16S rRNA gene sequence similarity, the closest phylogenetic relative was Bacillus shackletonii DSM 18868(T) (97.6 %). The DNA-DNA hybridization between GSS04(T) and its closest relatives revealed low relatedness (<70 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain GSS04(T) represents a novel species of the genus Bacillus, for which the name Bacillus dabaoshanensis sp. nov. is proposed. The type strain is GSS04(T) (=CCTCC AB 2013260(T) = KCTC 33191(T)).
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, 999 Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Cui
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Chang
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Zhuang
mesh_terms: D001407:Bacillus / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition / Q000235:genetics; D002308:Cardiolipins / Q000032:analysis; D002857:Chromium / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D005227:Fatty Acids / Q000032:analysis; D019216:Metals, Heavy; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism
chemical_list: D002308:Cardiolipins; D004269:DNA, Bacterial; D005227:Fatty Acids; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D002857:Chromium; C074702:chromium hexavalent ion