title: Association between polymorphisms of interleukin 10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis.
abstract: OBJECTIVE
  The aim of the present study was to examine the association between polymorphisms of IL-10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis (RA).
  
  
  METHODS
  We examined IL-10 rs1800872 A/C polymorphisms in 615 RA patients, and 839 controls, in an East Chinese Han population. Genotyping was performed using a custom-by-design 48-Plex SNP scan TM Kit. The blood plasma concentration of IL-10 was measured using an Iodine [(125)I] IL-10 Radioimmunoassay Kit, in 90 RA patients and 90 controls.
  
  
  RESULTS
  IL-10 rs1800872 A/C polymorphisms were associated with risk of RA. Following stratified analysis, an increased risk of RA was associated with the CC genotype among male, older, C-reactive protein-positive, anti-cyclic citrullinated peptide antibody-positive, and rheumatoid factor-positive-patients, and among patients with a DAS28 of≥3.20 or an erythrocyte sedimentation rate of≥25, and in functional class I and II patients. The average plasma concentration of IL-10 was significantly higher in RA patients compared with controls. RA patients positive for the homozygote CC were characterized by significantly higher levels of IL-10 compared with patients with the heterozygote AC. We also found that there were significant relationships between the single nucleotide polymorphisms in the human IL-10 rs1800872 A/C and production of IL-10.
  
  
  CONCLUSIONS
  Our results suggest that the IL-10 rs1800872 A/C allele might increase the risk of RA. The IL-10 rs1800872 A/C allele might also impact the inflammatory reaction of IL-10 in patients with RA.
authors.affiliation: Department of Orthopaedics, Changzhou First People's Hospital, Changzhou 213003, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ge
authors.affiliation: Department of Orthopaedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou 213003, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Orthopaedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou 213003, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopaedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou 213003, China; Central Laboratory, Changzhou Second People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. Electronic address: lrp216@sina.com.
authors.forename: Ruiping
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Orthopaedic Trauma, Affiliated Hospital of Nanjing Medical University, Changzhou Second People's Hospital, Changzhou 213003, China. Electronic address: xunanweimeta@hotmail.com.
authors.forename: Nanwei
authors.initials: N
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D015415:Biomarkers; D005260:Female; D060005:Genotyping Techniques; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide
chemical_list: D015415:Biomarkers; C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Interleukin 10; Molecular epidemiology; Polymorphisms; Rheumatoid arthritis