title: PFKFB4 controls embryonic patterning via Akt signalling independently of glycolysis.
abstract: How metabolism regulators play roles during early development remains elusive. Here we show that PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), a glycolysis regulator, is critical for controlling dorsal ectoderm global patterning in gastrulating frog embryos via a non-glycolytic function. PFKFB4 is required for dorsal ectoderm progenitors to proceed towards more specified fates including neural and non-neural ectoderm, neural crest or placodes. This function is mediated by Akt signalling, a major pathway that integrates cell homeostasis and survival parameters. Restoring Akt signalling rescues the loss of PFKFB4 in vivo. In contrast, glycolysis is not essential for frog development at this stage. Our study reveals the existence of a PFKFB4-Akt checkpoint that links cell homeostasis to the ability of progenitor cells to undergo differentiation, and uncovers glycolysis-independent functions of PFKFB4.
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Caterina
authors.initials: C
authors.lastname: Pegoraro
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Ana Leonor
authors.initials: AL
authors.lastname: Figueiredo
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Frédérique
authors.initials: F
authors.lastname: Maczkowiak
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Celio
authors.initials: C
authors.lastname: Pouponnot
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Eychène
authors.affiliation: 1] Université Paris Sud, Batiment 110, F-91 405 Orsay cedex, France [2] Institut Curie, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [3] CNRS UMR3347, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France [4] INSERM U1021, Centre Universitaire, Batiment 110, F-91 405 Orsay cedex, France.
authors.forename: Anne H
authors.initials: AH
authors.lastname: Monsoro-Burq
mesh_terms: D000818:Animals; D004625:Embryo, Nonmammalian / Q000196:embryology* / Q000201:enzymology*; D006019:Glycolysis / Q000235:genetics / Q000502:physiology; D051058:Oncogene Protein v-akt / Q000235:genetics / Q000378:metabolism*; D025481:Phosphofructokinase-2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D025481:Phosphofructokinase-2; D051058:Oncogene Protein v-akt