title: Protocols for the routine screening of drug sensitivity in the human parasite Trichomonas vaginalis.
abstract: Trichomonas vaginalis is a sexually transmitted protozoan parasite of humans. Treatment of trichomoniasis is almost completely dependent on the old drug metronidazole and is hampered by resistance. New drug development, like routine screening for drug resistance, has however been hampered by the lack of reliable screening protocols with sufficient throughput. Here we report on two separate in vitro protocols that use fluorescent dyes and allow for standardized drug sensitivity testing on the required scale.
authors.affiliation: Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, 120 University Place, Glasgow, G12 8TA, UK.
authors.forename: Manal J
authors.initials: MJ
authors.lastname: Natto
authors.forename: Anthonius A
authors.initials: AA
authors.lastname: Eze
authors.forename: Harry P
authors.initials: HP
authors.lastname: de Koning
mesh_terms: D000994:Antitrichomonal Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D021261:Parasitic Sensitivity Tests / Q000379:methods*; D014246:Trichomonas vaginalis / Q000187:drug effects*
chemical_list: D000994:Antitrichomonal Agents