title: Effects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goats.
abstract: Here we show the effects of repeated electroejaculation (EE) on mean values of motility, mitochondrial functionality, and expression of active caspases on goat sperm obtained by EE. Evaluations were done using CASA and flow cytometry. A strategy for identification of kinematic sperm subpopulations, when individual data of sperm are not provided by the CASA system, is provided. Fifty semen samples, five of each of ten adult creole goats, were obtained by electroejaculation. Mean values of total motility, progressive motility and flow cytometry evaluations were compared among EEs. Relationships among mean values of variables were investigated using Spearman correlation and principal component analysis (PCA). For identification of kinematic sperm subpopulations, PCA followed by hierarchical clustering was applied on data of the intervals provided automatically by the CASA system. Total motility does no change after repeated EE. Mean values of motility parameters and molecular markers were unrelated in multivariate space, but bivariate correlations were found. Values in upper and lower intervals defined clearly the sperm subpopulations, which had motility parameters changing over time. Taken together, our results show that repeated EE does not affect mean values of total motility, that molecular markers are not related with motility parameters, and that it is possible to identify kinematic sperm subpopulations when individual data, of motility parameters, are not provided by the CASA system.
authors.affiliation: Centro Universitario Temascaltepec, Universidad Autónoma del Estado de México, CP 50090 Toluca, Mexico.
authors.forename: A J F
authors.initials: AJ
authors.lastname: Vázquez
authors.affiliation: Centro Universitario Temascaltepec, Universidad Autónoma del Estado de México, CP 50090 Toluca, Mexico.
authors.forename: M J
authors.initials: MJ
authors.lastname: Cedillo
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
authors.forename: V J
authors.initials: VJ
authors.lastname: Quezada
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
authors.forename: A C
authors.initials: AC
authors.lastname: Rivas
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
authors.forename: E C L
authors.initials: EC
authors.lastname: Morales
authors.affiliation: Laboratorio de Pubertad, Facultad de Estudios Superiores Zaragoza, Universidad Nacional Autónoma de México, AP 9-020, CP 15000 Distrito Federal, Mexico.
authors.forename: E M E
authors.initials: EM
authors.lastname: Ayala
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
authors.forename: M J
authors.initials: MJ
authors.lastname: Hernández
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico.
authors.forename: R A
authors.initials: RA
authors.lastname: González
authors.affiliation: Laboratorio de Biología de la Reproducción, Facultad de Ingeniería y Ciencias, Universidad Autónoma de Tamaulipas, AP 337, CP 87149 Ciudad Victoria, Mexico. Electronic address: armandres@gmail.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Aragón
mesh_terms: D000818:Animals; D015415:Biomarkers; D004542:Ejaculation / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods*; D006041:Goats / Q000502:physiology*; D008297:Male; D008800:Mexico; D055101:Semen Analysis; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D015415:Biomarkers
keywords: Computer assisted semen analysis; Electroejaculation; Flow cytometry; Goat; Multivariate graphics; Multivariate statistics