title: Synergistic effect of laminin and mesenchymal stem cells on tracheal mucosal regeneration.
abstract: Although several studies have been successfully undertaken of tracheal reconstruction in terms of the maintaining the framework of the graft, most cases of reconstruction failure have resulted from delayed mucosal regeneration. The purposes of this study were to evaluate whether laminin-coated asymmetrically porous membrane (APM) scaffold enhances mucosal regeneration, to compare the mucosalization capability with mesenchymal stem cell (MSC) seeded APM, and to determine whether laminin coating and MSC seeding has a synergistic effect on mucosal regeneration. We reconstructed the full-thickness anterior tracheal defect of 36 New Zealand White rabbits with the APM scaffold. MSCs were isolated from the rabbit's inguinal fat. The animals were divided into 4 groups by the presence of laminin coating on APM and application of MSC [Group I, -/- (laminin/MSC); Group II, -/+; Group III, +/-; Group IV, +/+]. Endoscopy and histologic evaluation were performed and the results were compared among the groups. The results showed that ciliated columnar epithelium was regenerated earlier in groups II and III than in group I. Furthermore, the application of laminin and MSC had synergistic effects on tracheal epithelial regeneration. These results demonstrate that tracheal reconstruction by laminin-coated APM seeded with MSCs is most effective in enhancing tracheal mucosalization, and appears to be promising strategy in the regenerative treatment of tracheal defects.
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Doh Young
authors.initials: DY
authors.lastname: Lee
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, Republic of Korea.
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Hee-Jin
authors.initials: HJ
authors.lastname: Ahn
authors.affiliation: Department of Nanobiomedical Science and WCU Research Center, Dankook University, Cheonan, Republic of Korea.
authors.forename: Se Heang
authors.initials: SH
authors.lastname: Oh
authors.affiliation: Department of Advanced Materials, Hannam University, Daejeon, Republic of Korea.
authors.forename: Tae Ho
authors.initials: TH
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
authors.forename: Hee-Bok
authors.initials: HB
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
authors.forename: Seok-Won
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Republic of Korea; Cancer Research Institute, Seoul National University Hospital, Seoul, Republic of Korea; Medical Research Center, Seoul National University Hospital, Seoul, Republic of Korea. Electronic address: otolarynx@snuh.org.
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D058948:Cell Tracking; D002923:Cilia / Q000648:ultrastructure; D004724:Endoscopy; D005455:Fluorescent Antibody Technique; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D009092:Mucous Membrane / Q000166:cytology / Q000502:physiology*; D011817:Rabbits; D012038:Regeneration / Q000187:drug effects*; D013194:Staining and Labeling; D014132:Trachea / Q000187:drug effects / Q000502:physiology*
chemical_list: D007797:Laminin
keywords: Laminin; Mesenchymal stem cell; Mucosal regeneration; Reconstruction; Trachea