title: Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent.
abstract: A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6(T), was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1-1.5 µm in length and 0.5 µm in width. Strain TB-6(T) grew between 45 and 70 °C (optimum 55-60 °C), 0 and 35 g NaCl l(-1) (optimum 20-30 g l(-1)) and pH 4.5 and 7.5 (optimum pH 5.5-6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6(T) occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-d-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6(T) showed that this organism branched separately from the three most closely related genera, Caminibacter , Nautilia and Lebetimonas , within the family Nautiliaceae . Strain TB-6(T) contained several unique fatty acids in comparison with other members of the family Nautiliaceae . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae , Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6(T) ( = DSM 27783(T) = JCM 19563(T)).
authors.affiliation: Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901, USA.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Ashley
authors.initials: A
authors.lastname: Grosche
authors.affiliation: Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Hema
authors.initials: H
authors.lastname: Sekaran
authors.affiliation: Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901, USA.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Ileana
authors.initials: I
authors.lastname: Pérez-Rodríguez
authors.affiliation: Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
authors.forename: Valentin
authors.initials: V
authors.lastname: Starovoytov
authors.affiliation: Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901, USA.|Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, NJ 08901, USA.
authors.forename: Costantino
authors.initials: C
authors.lastname: Vetriani
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D020565:Epsilonproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D060086:Hydrothermal Vents / Q000382:microbiology*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7