title: MALDI-TOF MS for quality control of high protein content sport supplements.
abstract: High protein content sport nutritional supplements are found as powder products containing, as ingredients, amino acids and proteins with important nutritional values as milk, soy and egg proteins. An EU Food Supplements Directive (2002) requires that supplements should be safe, both in dosages and in purity. It is important, then, to develop rapid and sensitive methods to be employed for the quality control of these substances. In this work, we apply, for the first time, matrix-assisted laser desorption ionization-mass spectrometry as a fast, reproducible and sensitive method for the quality control of sport nutritional supplements based on proteins. To this aim, several commercial egg- and/or milk-based powder products have been processed by in gel or in solution digestion and analyzed in comparison to pure standard products. This strategy allowed to assess the reliability of the indications on proteins (as caseins, whey proteins and ovalbumin) declared in the label of several sport nutritional supplements.
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona, 4, 70126 BARI, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: De Ceglie
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona, 4, 70126 BARI, Italy. Electronic address: cosimadamiana.calvano@uniba.it.
authors.forename: Cosima D
authors.initials: CD
authors.lastname: Calvano
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Bari "Aldo Moro", Via Orabona, 4, 70126 BARI, Italy; Centro Interdipartimentale di Ricerca "S.M.A.R.T.", Università degli Studi di Bari "Aldo Moro", Via Orabona, 4, 70126 BARI, Italy.
authors.forename: Carlo G
authors.initials: CG
authors.lastname: Zambonin
mesh_terms: D000818:Animals; D054874:Athletic Performance; D019587:Dietary Supplements; D008892:Milk / Q000737:chemistry*; D011208:Powders; D011786:Quality Control; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013177:Sports
chemical_list: D011208:Powders
keywords: Egg; High quality proteins; MALDI; Milk; Sport supplements