title: Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.
abstract: Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production.
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Javier A
authors.initials: JA
authors.lastname: Linares-Pastén
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Ramin
authors.initials: R
authors.lastname: Sabet-Azad
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Laura
authors.initials: L
authors.lastname: Pessina
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Roya R R
authors.initials: RR
authors.lastname: Sardari
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden; Chemistry of Natural and Microbial Products Department, National Research Centre, Al-Bohoos St., 12622 Cairo, Egypt.
authors.forename: Mohammad H A
authors.initials: MH
authors.lastname: Ibrahim
authors.affiliation: Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: Rajni.Hatti-Kaul@biotek.lu.se.
authors.forename: Rajni
authors.initials: R
authors.lastname: Hatti-Kaul
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D002861:Chromobacterium / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000378:metabolism*; D030781:Organisms, Genetically Modified; D011091:Polyesters / Q000378:metabolism*; D011407:Propane / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins
chemical_list: D000217:Acyltransferases; D000445:Aldehyde Oxidoreductases; D005985:Glyceraldehyde; D005990:Glycerol; D011091:Polyesters; D011407:Propane; D011994:Recombinant Proteins; C000600150:poly(3-hydroxypropionate); C047158:3-hydroxypropionaldehyde; C588134:coenzyme A-acylating propionaldehyde dehydrogenase, Lactobacillus reuteri; D001426:Bacterial Proteins; C077225:poly(3-hydroxyalkanoic acid) synthase
keywords: Coenzyme A-acylating propionaldehyde dehydrogenase; Lactobacillus reuteri; Poly(3-hydroxypropionate); Polyhydroxyalkanoate synthase; Reuterin