title: Comparison of mitochondrial-related transcriptional levels of TFAM, NRF1 and MT-CO1 genes in single human oocytes at various stages of the oocyte maturation.
abstract: The aim of the current study was to assess the mRNA levels of two mitochondria-related genes, including nuclear-encoded NRF1 (nuclear respiratory factor 1), mitochondrial transcription factor A (TFAM), and mitochondrial-encoded cytochrome c oxidase subunit 1 (MT-CO1) genes in various stages of the human oocyte maturation.
  
  
  
  Oocytes were obtained from nine infertile women with male factor undergoing in vitro fertilization (IVF)/intra-cytoplasmic sperm injection protocol. Mitochondrial-related mRNA levels were performed by single-cell TaqMan real-time PCR.
  
  
  
  the expression level of the target genes was low at the germinal vesicle stage (P>0.05). Although the mRNA level of NRF1gene remained stable in metaphase I, the mRNA level of TFAM and MT-CO1 increased significantly (P<0.05).In metaphase II, the expression level of all genes increased compared to metaphase I (P<0.05).
  
  
  
  The overexpression levels of NRF1, TFAM, and MT-CO1 genes are related to the oocyte maturation. Therefore, the current study could be used clinically to improve the success rate of IVF.
authors.affiliation: Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
authors.forename: Marefat
authors.initials: M
authors.lastname: Ghaffari Novin
authors.affiliation: Dept. of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Noruzinia
authors.affiliation: Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
authors.forename: Azra
authors.initials: A
authors.lastname: Allahveisi
authors.affiliation: Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
authors.forename: Aboutaleb
authors.initials: A
authors.lastname: Saremi
authors.affiliation: Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
authors.forename: Fateme
authors.initials: F
authors.lastname: Fadaei Fathabadi
authors.affiliation: Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mastery Farahani
authors.affiliation: Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Dehghani Fard
authors.affiliation: Sarem Cell Research Center (SCRC), Sarem Women’s Hospital, Tehran, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Pooladi
authors.affiliation: Rastegar Central Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran,  Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Mazaherinezhad Fard
authors.affiliation: Dept. of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Yousefian
mesh_terms: D000328:Adult; D004272:DNA, Mitochondrial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D020869:Gene Expression Profiling; D006801:Humans; D007247:Infertility, Female; D008297:Male; D008677:Metaphase / Q000235:genetics; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000235:genetics*; D051266:Nuclear Respiratory Factor 1 / Q000096:biosynthesis / Q000235:genetics*; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D000077278:RNA, Mitochondrial; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D055815:Young Adult
chemical_list: D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; C495633:NRF1 protein, human; D051266:Nuclear Respiratory Factor 1; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C499631:TFAM protein, human; D014157:Transcription Factors; C029305:mitochondrial messenger RNA; D003576:Electron Transport Complex IV; C581860:cytochrome c oxidase subunit I, human
keywords: Transcription; Human oocyte; Real-time PCR