title: α-Hemoglobin-stabilizing Protein: An Effective Marker for Erythroid Precursors in Bone Marrow Biopsy Specimens.
abstract: Accurate analysis of the erythroid lineage is essential in evaluating bone marrow biopsies and can be particularly challenging in settings of dyserythropoiesis. α-Hemoglobin-stabilizing protein (AHSP) is an erythroid-specific chaperone protein and represents a potential specific marker for erythroid elements. This study defines the immunohistochemical profile of AHSP, as compared with an established erythroid marker CD71, in 101 bone marrow biopsies including normal marrows and cases of acute pure erythroid leukemia, acute erythroid/myeloid leukemia, other types of acute myeloid leukemia, myelodysplastic syndrome, chronic myelogenous leukemia, other types of myeloproliferative neoplasm, chronic myelomonocytic leukemia, acute lymphoblastic leukemia, plasma cell neoplasm, and metastatic carcinoma. In acute pure erythroid leukemia, blasts in 7 of 11 cases showed similar reactivity for CD71 and AHSP, whereas less extensive reactivity was observed for AHSP as compared with CD71 in the remaining 4 cases. In normal marrows and other various disorders, reactivity for AHSP was similar to CD71 and was restricted to the erythroid lineage. Mature erythrocytes were negative for AHSP as were myeloblasts, lymphoblasts, nonerythroid hematopoietic marrow elements, plasma cells, and carcinoma cells. AHSP is an effective marker for detection of normal or abnormal erythroid precursors in bone marrow biopsies and is a useful addition to an immunohistochemical panel for assessment of neoplastic cells of possible erythroid derivation.
authors.affiliation: *Department of Pathology, UMass Memorial Medical Center and University of Massachusetts Medical School, Worcester †Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.forename: Jack L
authors.initials: JL
authors.lastname: Pinkus
authors.forename: Geraldine S
authors.initials: GS
authors.lastname: Pinkus
mesh_terms: D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D044963:Biopsy, Fine-Needle; D001798:Blood Proteins / Q000235:genetics* / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D041905:Erythroid Cells / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D019337:Hematologic Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000378:metabolism / Q000473:pathology; D018832:Molecular Chaperones / Q000235:genetics* / Q000378:metabolism; D009196:Myeloproliferative Disorders / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011990:Receptors, Transferrin / Q000235:genetics* / Q000378:metabolism
chemical_list: C468768:AHSP protein, human; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D001798:Blood Proteins; C068126:CD71 antigen; D018832:Molecular Chaperones; D011990:Receptors, Transferrin