title: [Mitochondrial estrogen receptor β inhibits non-small cell lung cancer cell apoptosis via interaction with Bad].
abstract: OBJECTIVE
  To explore the molecular mechanisms by which mitochondrial estrogen receptor β (ERβ) suppresses non-small cell lung cancer cell apoptosis induced by apoptotic stimulations.
  
  
  METHODS
  The mitochondrial localization of ERβ in non-small cell lung cancer cell lines A549 and 201T was determined using immunofluorescence and Western blotting. The changes of apoptosis of the cells with mitochondrial ERβ overexpression or knockdown in response to cisplatin and STS treatments were assessed, and mitochondrial ERβ interaction with the pro-apoptotic protein Bad was detected using co-immunoprecipitation and Western blotting.
  
  
  RESULTS
  ERβ was localized in the mitochondria in A549 and 201T cells. ERβ overexpression significantly reduced while ERβ knockdown increased Bax activation and cell apoptosis induced by cisplatin and STS. Mitochondrial ERβ interaction with pro-apoptotic protein Bad may suppress Bax activation and its translocation to the mitochondria.
  
  
  CONCLUSIONS
  Mitochondrial ERβ can suppress apoptosis of non-small cell lung cancer cells induced by cisplatin or STS through interaction with Bad, suggesting the value of mitochondrial ERβ as a new therapeutic target for treatment of non-small cell lung cancer.
authors.affiliation: Armed Police Corps Hospital of Guangdong Province, Guangzhou 510507 China.E-mail: doctorxie@126.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xie
authors.forename: Zuoping
authors.initials: Z
authors.lastname: Huang
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology*; D045744:Cell Line, Tumor; D002945:Cisplatin; D047629:Estrogen Receptor beta / Q000378:metabolism*; D006801:Humans; D024101:Mitochondrial Proteins / Q000378:metabolism*; D051025:bcl-Associated Death Protein / Q000378:metabolism*
chemical_list: C494819:BAD protein, human; C000600034:ESR2 protein, human; D047629:Estrogen Receptor beta; D024101:Mitochondrial Proteins; D051025:bcl-Associated Death Protein; D002945:Cisplatin