title: Light acclimation optimizes leaf functional traits despite height-related constraints in a canopy shading experiment.
abstract: Within-canopy gradients of leaf functional traits have been linked to both light availability and vertical gradients in leaf water potential. While observational studies can reveal patterns in leaf traits, within-canopy experimental manipulations can provide mechanistic insight to tease apart multiple interacting drivers. Our objectives were to disentangle effects of height and light environment on leaf functional traits by experimentally shading branches along vertical gradients within a sugar maple (Acer saccharum) forest. Shading reduced leaf mass per area (LMA), leaf density, area-based leaf nitrogen (N(area)), and carbon:nitrogen (C:N) ratio, and increased mass-based leaf nitrogen (N(mass)), highlighting the importance of light availability on leaf morphology and chemistry. Early in the growing season, midday leaf water potential (Ψ(mid)), LMA, and N(area) were driven primarily by height; later in the growing season, light became the most important driver for LMA and Narea. Carbon isotope composition (δ(13)C) displayed strong, linear correlations with height throughout the growing season, but did not change with shading, implying that height is more influential than light on water use efficiency and stomatal behavior. LMA, leaf density, N(mass), C:N ratio, and δ(13)C all changed seasonally, suggesting that leaf ageing effects on leaf functional traits are equally as important as microclimatic conditions. Overall, our results indicate that: (1) stomatal sensitivity to vapor pressure deficit or Ψ(mid) constrains the supply of CO2 to leaves at higher heights, independent of light environment, and (2) LMA and N(area) distributions become functionally optimized through morphological acclimation to light with increasing leaf age despite height-related constraints.
authors.affiliation: School of Forest Resources and Environmental Science, Michigan Technological University, U.J. Noblet Building, 1400 Townsend Dr., Houghton, MI, 49931, USA, apcoble@mtu.edu.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Coble
authors.forename: Molly A
authors.initials: MA
authors.lastname: Cavaleri
mesh_terms: D000064:Acclimatization; D031002:Acer / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000378:metabolism; D003624:Darkness; D008027:Light; D009584:Nitrogen / Q000378:metabolism; D010641:Phenotype; D010788:Photosynthesis; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D013312:Stress, Physiological; D014197:Trees / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D014867:Water / Q000502:physiology
chemical_list: D002247:Carbon Isotopes; D014867:Water; D002245:Carbon Dioxide; D002244:Carbon; D009584:Nitrogen