title: Effects of a lyophilized aqueous extract of Feretia apodanthera Del. (Rubiaceae) on pentylenetetrazole-induced kindling, oxidative stress, and cognitive impairment in mice.
abstract: Feretia apodanthera Del. (Rubiaceae) is extensively used in ethnomedicine in Cameroon and Nigeria for epilepsy, febrile convulsions, and rheumatic pains and for enhancing cognitive performance. The aim of the present study was to examine the effects of a lyophilized aqueous extract of F. apodanthera on the course of kindling development, kindling-induced learning deficit, oxidative stress markers, and cholinesterase activity in pentylenetetrazole (PTZ)-kindled mice. Pentylenetetrazole, 30mg/kg, induced kindling in mice after 30.00±1.67days. The aqueous extract of F. apodanthera showed dose-dependent antiseizure effects. Feretia apodanthera (150-200mg/kg) significantly increased the latency to myoclonic jerks, clonic seizures, and generalized tonic-clonic seizures. The extract also improved the seizure score and decreased the number of myoclonic jerks. Pentylenetetrazole kindling induced significant oxidative stress and cognitive impairment which were reversed by pretreatment with F. apodanthera in a dose-dependent manner. The significant decrease in cholinesterase activity observed in the PTZ-kindled mice was reversed by pretreatment with the F. apodanthera extract. The results indicated that pretreatment with the aqueous extract of F. apodanthera antagonizes seizures, oxidative stress, and cognitive impairment in PTZ-kindled mice. The aqueous extract of F. apodanthera also showed anxiolytic activities, but the inhibition of memory impairment was not attributed to the anxiolytic activities of the plant. These results thus suggest the potential of F. apodanthera as an adjuvant in epilepsy both to prevent seizures as well as to protect against seizure-induced oxidative stress and memory impairment.
authors.affiliation: Department of Zoology and Animal Physiology, Faculty of Sciences, University of Buea, P.O. Box 63, Buea, Cameroon.
authors.forename: G S
authors.initials: GS
authors.lastname: Taiwe
authors.affiliation: Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.
authors.forename: F C O
authors.initials: FC
authors.lastname: Moto
authors.affiliation: Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.
authors.forename: E R M
authors.initials: ER
authors.lastname: Ayissi
authors.affiliation: Department of Animal Biology, Faculty of Sciences, University of Dschang, P.O. Box 67, Dschang, Cameroon.
authors.forename: G T
authors.initials: GT
authors.lastname: Ngoupaye
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.
authors.forename: J S K
authors.initials: JS
authors.lastname: Njapdounke
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.
authors.forename: G C N
authors.initials: GC
authors.lastname: Nkantchoua
authors.affiliation: Department of Zoology and Animal Physiology, Faculty of Sciences, University of Buea, P.O. Box 63, Buea, Cameroon.
authors.forename: N
authors.initials: N
authors.lastname: Kouemou
authors.affiliation: Department of Biological Sciences, Higher Teachers' Training College, University of Yaoundé I, P.O. Box 47, Yaoundé, Cameroon.
authors.forename: J P O
authors.initials: JP
authors.lastname: Omam
authors.affiliation: Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: A K
authors.initials: AK
authors.lastname: Kandeda
authors.affiliation: Department of Zoology and Animal Physiology, Faculty of Sciences, University of Buea, P.O. Box 63, Buea, Cameroon.
authors.forename: S
authors.initials: S
authors.lastname: Pale
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon.
authors.forename: D
authors.initials: D
authors.lastname: Pahaye
authors.affiliation: Department of Biological Sciences, Faculty of Science, University of Ngaoundéré, P.O. Box 454, Ngaoundéré, Cameroon. Electronic address: eli_bum@yahoo.fr.
authors.forename: E
authors.initials: E
authors.lastname: Ngo Bum
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001007:Anxiety / Q000523:psychology; D003071:Cognition / Q000187:drug effects*; D003072:Cognition Disorders / Q000188:drug therapy* / Q000523:psychology*; D003292:Convulsants; D005612:Freeze Drying; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D010433:Pentylenetetrazole; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011597:Psychomotor Performance / Q000187:drug effects; D019666:Rubiaceae / Q000737:chemistry*; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control / Q000523:psychology
chemical_list: D003292:Convulsants; D010936:Plant Extracts; D000110:Acetylcholinesterase; D010433:Pentylenetetrazole
keywords: Cholinesterase activity; Cognitive impairment; Feretia apodanthera; Kindling development; Oxidative stress