title: High resolution LC-ESI-TOF-mass spectrometry method for fast separation, identification, and quantification of 12 isoflavones in soybeans and soybean products.
abstract: A high resolution LC-ESI-TOF-MS analytical method was established for the rapid isolation, identification, and quantification of 12 isoflavones in soybean and soybean products. Individual isoflavones were identified on the basis of the accurate mass data of their respective protonated mass ions, Na or K adduct ions, fragment ions, and isotope ion patterns. The protonated mass ions of isoflavones were extracted for their quantification in soybean products. Twelve different isoflavones in the soybean products were fully separated, identified and quantified within 12min separation time. The established LC-TOF/MS was an effective analytical method for the simultaneous characterization and quantification of isoflavones with exceptionally short analytical time, high selectivity, a high linearity (r(2)>0.992) in a wide range, low LOD and LOQ, high precision, inter-and intra-day repeatability, and no significant matrix effect. Furthermore, it requires simple sample preparation procedure (solvent extraction, dilution, and syringe filtration).
authors.affiliation: College of Food Science, Woosuk University, Samrea-Up, Wanju-Kun, Jeonbuk Province 565-701, Republic of Korea.
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Lee
authors.affiliation: Department of Applied Life Science, College of Life and Environment Science, Konkuk University, Seoul, Republic of Korea.
authors.forename: Ill-Min
authors.initials: IM
authors.lastname: Chung
authors.affiliation: Food Research R&D Center, Nongshim Food Co., Seoul, Republic of Korea.
authors.forename: Hunjung
authors.initials: H
authors.lastname: Kim
authors.affiliation: College of Food Science, Woosuk University, Samrea-Up, Wanju-Kun, Jeonbuk Province 565-701, Republic of Korea. Electronic address: munjung@woosuk.ac.kr.
authors.forename: Mun Yhung
authors.initials: MY
authors.lastname: Jung
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007529:Isoflavones / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D013025:Glycine max / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D007529:Isoflavones
keywords: High performance liquid chromatography; Isoflavones; Soybean; Time-of-flight mass spectrometry