title: Anthocyanins and antioxidant capacities of six Chilean berries by HPLC-HR-ESI-ToF-MS.
abstract: The HPLC profiles of six fruits endemic of the VIII region of Chile were investigated using high resolution mass analysis (HR-ToF-ESI-MS). The anthocyanin fingerprints generated for the fruits were compared and the antioxidant capacities measured by the scavenging of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and correlated with the inhibition of lipid peroxidation in human erythrocytes (LP) and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Several anthocyanins were identified, including 3-O-glycosides derivatives of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloyl-quinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity. However, the highest LP activity was found for Chilean blueberries (>95%) followed by calafate fruits (91.27%) and luma (83.4%).
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta, Chile.
authors.forename: Javier E
authors.initials: JE
authors.lastname: Ramirez
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta, Chile.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Zambrano
authors.affiliation: Departamento de Ciencias Químicas, Universidad Andrés Bello, Campus Viña del Mar, Los Fresnos N 52, Viña del Mar 2520000, Chile.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sepúlveda
authors.affiliation: Department of Biological Sciences, Lehman College and The Graduate Center, The City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, United States.
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Kennelly
authors.affiliation: Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias Básicas, Universidad de Antofagasta, Av. Coloso S-N, Antofagasta, Chile. Electronic address: mario.simirgiotis@uantof.cl.
authors.forename: Mario J
authors.initials: MJ
authors.lastname: Simirgiotis
mesh_terms: D000872:Anthocyanins / Q000737:chemistry* / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D002677:Chile; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D027822:Myrtaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D010936:Plant Extracts
keywords: Anthocyanins; Antioxidant capacity; Chilean berries; HPLC–MS healthy edible fruits; Phenolics