title: Molecular characterization and RNA interference analysis of vitellogenin receptor from Nilaparvata lugens (Stål).
abstract: Vitellogenin receptors (VgRs), members of the low-density lipoprotein receptor (LDLR) superfamily, are responsible for taking vitellogenin (Vg) into developing oocytes. Here the first full-length VgR cDNA from a hemipteran insect, the brown planthopper (Nilaparvata lugens), was cloned and sequenced. The complete mRNA sequence was 6174 bp in length with an open reading frame (ORF) of 5796 bp encoding 1931 amino acid residues. N. lugens VgR (NlVgR) contained two ligand-binding domains with five LDLR Class A cysteine-rich repeats in the first domain and eight in the second domain, which was similar to other insect VgRs. NlVgR was specifically expressed in the ovary, and the mRNA level started to increase after adult female emergence, with a peak on day 7 in the adult stage, and then declined. Western blot analysis of NlVgR protein revealed an ovary-specific expression pattern, which was consistent with NlVgR transcript detection. Injection with NlVgR double-stranded RNA (dsRNA) significantly disturbed NlVgR, which led to a decrease in NlVg protein content in the ovaries, an accumulation of NlVg protein in the hemolymph, the arrested development of ovaries, and the failure of insects to reproduce. Besides, NlVgR expression was significantly upregulated after the topical application of juvenile hormone (JH) III. These results suggest that VgR is critical for Vg uptaking of oocytes and it plays an important role in insect fecundity.
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Shu
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Mingxiao
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China. Electronic address: lsszhou@mail.sysu.edu.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Biocontrol and Institute of Entomology, Sun-Yat-sen University, Guangzhou 510275, China.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D018076:DNA, Complementary / Q000032:analysis; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005819:Genetic Markers; D006430:Hemiptera / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D010802:Phylogeny; D034622:RNA Interference; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D004527:Egg Proteins; D005819:Genetic Markers; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor
keywords: Juvenile hormone; Low density lipoprotein receptor; Nilaparvata lugens; RNAi; Reproduction; Vitellogenin receptor