title: In vitro detection of circulating tumor cells compared by the CytoTrack and CellSearch methods.
abstract: Comparison of two methods to detect circulating tumor cells (CTC) CytoTrack and CellSearch through recovery of MCF-7 breast cancer cells, spiked into blood collected from healthy donors. Spiking of a fixed number of EpCAM and pan-cytokeratin positive MCF-7 cells into 7.5 mL donor blood was performed by FACSAria flow sorting. The samples were shipped to either CytoTrack or CellSearch research facilities within 48 h, where evaluation of MCF-7 recovery was performed. CytoTrack and CellSearch analyses were performed simultaneously. Recoveries of MCF-7 single cells, cells in clusters, and clusters were determined. The average numbers of MCF-7 cells/cells in clusters/clusters recovered from blood by the CytoTrack and CellSearch methods were 103 ± 5.9/27 ± 7.9/11 ± 3.5 (95 % CI) and 107 ± 4.4/20 ± 7.1/10 ± 3.5, respectively, with no difference between the two methods (p = 0.37/p = 0.23/p = 0.09). Overall, the recovery of CytoTrack and CellSearch was 68.8 ± 3.9 %/71.1 ± 2.9 %, respectively (p = 0.58). In spite of different methodologies, CytoTrack and CellSearch found similar number of CTCs, when spiking was performed with the EpCAM and pan cytokeratin-positive cell line MCF-7. The results suggest that CytoTrack and CellSearch have similar abilities to identify CTC in vitro.
authors.affiliation: Department of Clinical Biochemistry, Nordsjællands Hospital, Hillerød, University of Copenhagen, 3400, Hillerød, Denmark, thore.hillig@regionh.dk.
authors.forename: Thore
authors.initials: T
authors.lastname: Hillig
authors.forename: Peer
authors.initials: P
authors.lastname: Horn
authors.forename: Ann-Britt
authors.initials: AB
authors.lastname: Nygaard
authors.forename: Anastasiya S
authors.initials: AS
authors.lastname: Haugaard
authors.forename: Sarah
authors.initials: S
authors.lastname: Nejlund
authors.forename: Ivan
authors.initials: I
authors.lastname: Brandslund
authors.forename: György
authors.initials: G
authors.lastname: Sölétormos
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics*; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006801:Humans; D066298:In Vitro Techniques / Q000379:methods; D007633:Keratins / Q000737:chemistry / Q000235:genetics; D061986:MCF-7 Cells; D009360:Neoplastic Cells, Circulating; D011379:Prognosis
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D007633:Keratins