title: Investigation of the monocyte diapedesis-related LFA-1 and JAM-A gene variants in Turkish coronary heart disease patients.
abstract: BACKGROUND
  LFA-1/JAM-A interaction plays a significant role in early steps of leukocyte transendothelial migration (diapedesis) which takes part in atherosclerosis pathogenesis. In this population-based case-control study, the frequencies of JAM-A rs790056 and LFA-1 rs8058823 gene polymorphisms in patients with coronary heart disease (CHD) and healthy subjects were investigated and the correlations between the different genotypes and cardiovascular risk factors were analyzed.
  
  
  METHODS
  The JAM-A and LFA-1 genotypes were determined in 153 patients with CHD and 124 controls by PCR-RFLP assay.
  
  
  RESULTS
  In CHD patient group, the frequency of JAM-A rs790056 TT genotype and the frequency of T allele were higher when compared with the control group (p = 0.03 and p = 0.007,respectively). In patient groups, the frequency of LFA-1 rs8058823 AA genotype was higher (p = 0.000), and the frequency of AG genotype was lower when compared with the control group (p = 0.031). In the control group, LFA-1 rs8058823 G allele carriers had higher SBP than subjects with AA genotype (p = 0.038), whereas in the CHD patient group, G allele carriers had lower DBP than subjects with AA genotype (p = 0.007). The multivariate logistic regression analysis confirmed that the JAM-A rs790056 TT genotype (OR = 2.472, p = 0.045) and LFA-1 rs8058823 AA genotype (OR = 6.751, p = 0.000) were risk factors for CHD development.
  
  
  CONCLUSIONS
  These results suggest that the wild type genotypes and alleles of JAM-A rs790056 (TT genotype and T allele) and LFA-1 rs8058823 (AA genotype and A allele) were found to be risk factors for CHD, whereas rare genotypes and alleles were found to be higher in healthy controls thus being protective.
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Bengu
authors.initials: B
authors.lastname: Tokat
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Kurt
authors.affiliation: Department of Cardiology, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkey.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Bugra
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Oguz
authors.initials: O
authors.lastname: Ozturk
authors.affiliation: Department of Molecular Medicine, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Yilmaz-Aydogan
keywords: 3′UTR, 3′-untranslated region; BMI, body mass index; CHD, coronary heart disease; CI, confidence interval; Coronary heart disease; DBP, diastolic blood pressure; Diapedesis; HDL-C, high density lipoprotein cholesterol; HWE, Hardy-Weinberg Equilibrium; JAM-A; JAM-A, junctional adhesion molecule-A; LDL-C, low density lipoprotein cholesterol; LFA-1; LFA-1, leukocyte function-associated antigen-1; LVH, left ventricular hypertrophy; OR, odds ratio; PCR-RFLP, polymerase chain reaction–restriction fragment length polymorphism; Polymorphism; SBP, systolic blood pressure; SNP, single nucleotide polymorphism; T2DM, type 2 diabetes mellitus; TC, total cholesterol; TG, triglyceride; VLDL-C, very low density lipoprotein cholesterol