title: Karyotype, C-banding and AgNORs of two endemic leuciscine fish, Pseudophoxinuscrassus (Ladiges, 1960) and P.hittitorum Freyhof & Özulug, 2010 (Teleostei, Cyprinidae).
abstract: The genus Pseudophoxinus Bleeker, 1860 is found in a wide range of habitats in central Anatolia, but it is not well known from a cytogenetic aspect. In this study the first karyotypic description of the spring minnows Pseudophoxinuscrassus (Ladiges, 1960) and Pseudophoxinushittitorum Freyhof & Özulug, 2010 by means of conventional methods (Giemsa staining, C-banding, silver nitrate impregnation (Ag-NORs)) was performed. Both species are endemic and have restricted distributions in Central Anatolia. Pseudophoxinuscrassus and Pseudophoxinushittitorum have the same diploid chromosome number, 2n = 50, patterns of distribution of constitutive heterochromatin (CH), and localization of nucleolus organizer regions (NORs), but differ in their karyotypic formulae (KFs). The C-banding technique revealed clear pericentromeric blocks of CH in many chromosomes; Ag-NORs treatment revealed consistent positive signals at the end of the short arms of a submetacentric chromosome pair, likely homologous in both species. The karyotypic differences found between these species can be used for their taxonomical study.
authors.affiliation: Gazi University, Science Faculty, Department of Biology, Ankara, Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Unal
authors.affiliation: Ahi Evran University, Science and Art Faculty, Department of Biology, Kırşehir, Turkey.
authors.forename: Muhammet
authors.initials: M
authors.lastname: Gaffaroğlu
authors.affiliation: Ahi Evran University, Science and Art Faculty, Department of Biology, Kırşehir, Turkey.
authors.forename: Muradiye Karasu
authors.initials: MK
authors.lastname: Ayata
authors.affiliation: Gazi University, Science Faculty, Department of Biology, Ankara, Turkey.
authors.forename: Eşref
authors.initials: E
authors.lastname: Yüksel
keywords: C-banding; Karyotype; Leuciscinae; NOR-phenotype; cytotaxonomy