title: Immunofluorescence microtip sensor for point-of-care tuberculosis (TB) diagnosis.
abstract: A immunofluorescence microtip sensor was developed for specific detection of Mycobacterium cells in sputum samples by the combination of electric field, streaming flow, and immuno-affinity binding. The detection limit was 200 CFU/mL in human sputum, which was comparable to PCR but without requiring bacteriological culture, centrifugation, or nucleic acid amplification. In spite of the complex nature of physical, chemical, and biological mechanisms, the simple operation of "dipping and withdrawal" of tips will allow for screening by minimally trained personnel within 30 min. In addition, the minimal power requirement (5 W) combined with low assay cost is ideal for point-of-care (POC) screening in resource-limited settings.
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Kyong-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Gerard A
authors.initials: GA
authors.lastname: Cangelosi
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Chung
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004560:Electricity; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008839:Microelectrodes; D055616:Microtechnology / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D036103:Nanotechnology / Q000295:instrumentation*; D019095:Point-of-Care Systems; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate