title: Regulation of nutrition-associated receptors in blood monocytes of normal weight and obese humans.
abstract: Obesity, type 2 diabetes and associated metabolic diseases are characterized by low-grade systemic inflammation which involves interplay of nutrition and monocyte/macrophage functions. We suggested that some factors such as nutrient components, neuropeptides involved in the control of gastrointestinal functions, and gastrointestinal hormones might influence immune cell functions and in this way contribute to the disease pathogenesis. The aim of this study was to investigate the mRNA expression of twelve nutrition-associated receptors in peripheral blood mononuclear cells (PBMC), isolated monocytes and monocyte-derived macrophages and their regulation under the switching from the high-carbohydrate low-fat diet to the low-carbohydrate high-fat (LC/HFD) isocaloric diet in healthy humans. The mRNA expression of receptors for short chain fatty acids (GPR41, GPR43), bile acids (TGR5), incretins (GIPR, GLP1R), cholecystokinin (CCKAR), neuropeptides VIP and PACAP (VIPR1, VIPR2), and neurotensin (NTSR1) was detected in PBMC and monocytes, while GPR41, GPR43, GIPR, TGR5, and VIPR1 were found in macrophages. Correlations of the receptor expression in monocytes with a range of metabolic and inflammatory markers were found. In non-obese subjects, the dietary switch to LC/HFD induced the increase of GPR43 and VIPR1 expression in monocytes. No significant differences of receptor expression between normal weight and moderately obese subjects were found. Our study characterized for the first time the expression pattern of nutrition-associated receptors in human blood monocytes and its dietary-induced changes linking metabolic responses to nutrition with immune functions in health and metabolic diseases.
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany. Electronic address: olga.pivovarova@dife.de.
authors.forename: Olga
authors.initials: O
authors.lastname: Pivovarova
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
authors.forename: Silke
authors.initials: S
authors.lastname: Hornemann
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Weimer
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.
authors.forename: Ye
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.
authors.forename: Veronica
authors.initials: V
authors.lastname: Murahovschi
authors.affiliation: Almazov Federal Medical Research Centre, Saint-Petersburg, Russian Federation.
authors.forename: Sergei
authors.initials: S
authors.lastname: Zhuk
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
authors.forename: Anne-Cathrin
authors.initials: AC
authors.lastname: Seltmann
authors.affiliation: Almazov Federal Medical Research Centre, Saint-Petersburg, Russian Federation.
authors.forename: Anna
authors.initials: A
authors.lastname: Malashicheva
authors.affiliation: Almazov Federal Medical Research Centre, Saint-Petersburg, Russian Federation.
authors.forename: Anna
authors.initials: A
authors.lastname: Kostareva
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Kruse
authors.affiliation: HealthTwiSt GmbH, Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Busjahn
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.
authors.forename: Natalia
authors.initials: N
authors.lastname: Rudovich
authors.affiliation: Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; Department of Endocrinology, Diabetes and Nutrition, Campus Benjamin Franklin, Charité University Medicine, Berlin, Germany.
authors.forename: Andreas F H
authors.initials: AF
authors.lastname: Pfeiffer
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004041:Dietary Fats / Q000008:administration & dosage*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D054795:Incretins / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics / Q000378:metabolism; D009765:Obesity / Q000097:blood / Q000235:genetics*; D009928:Organ Specificity; D061251:Primary Cell Culture; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism; D018028:Receptors, Neurotensin / Q000235:genetics / Q000378:metabolism
chemical_list: D002766:Cholecystokinin; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D054795:Incretins; D009479:Neuropeptides; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D018028:Receptors, Neurotensin; C473103:FFAR2 protein, human; C106262:FFAR3 protein, human; C473772:GPBAR1 protein, human; C114036:neurotensin type 1 receptor
keywords: High-fat diet; Human nutrition-associated receptors; Monocytes; Obesity