title: Whole genome sequencing of clinical isolates of Giardia lamblia.
abstract: Clinical isolates from protozoan parasites such as Giardia lamblia are at present practically impossible to culture. By using simple cyst purification methods, we show that Giardia whole genome sequencing of clinical stool samples is possible. Immunomagnetic separation after sucrose gradient flotation gave superior results compared to sucrose gradient flotation alone. The method enables detailed analysis of a wide range of genes of interest for genotyping, virulence and drug resistance.
authors.affiliation: Department of Clinical Science, University of Bergen, Bergen, Norway; National Center for Tropical Infectious Diseases, Department of Medicine, Bergen, Norway. Electronic address: kurt.hanevik@med.uib.no.
authors.forename: K
authors.initials: K
authors.lastname: Hanevik
authors.affiliation: Department of Clinical Science, University of Bergen, Bergen, Norway.
authors.forename: R
authors.initials: R
authors.lastname: Bakken
authors.affiliation: Department of Clinical Science, University of Bergen, Bergen, Norway; Center for Medical Genetics and Molecular Medicine, Bergen, Norway.
authors.forename: H R
authors.initials: HR
authors.lastname: Brattbakk
authors.affiliation: Department of Clinical Science, University of Bergen, Bergen, Norway.
authors.forename: C S
authors.initials: CS
authors.lastname: Saghaug
authors.affiliation: Department of Clinical Science, University of Bergen, Bergen, Norway; Department of Medicine, Haukeland University Hospital, Bergen, Norway.
authors.forename: N
authors.initials: N
authors.lastname: Langeland
mesh_terms: D005243:Feces / Q000469:parasitology; D018503:Genome, Protozoan; D016829:Giardia lamblia / Q000235:genetics* / Q000302:isolation & purification; D005873:Giardiasis / Q000469:parasitology*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D010274:Parasitology / Q000379:methods*; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000379:methods*
keywords: Clinical isolates; Giardia; immunomagnetic separation; sucrose gradient flotation; whole genome sequencing