title: Carboxylic group-induced synthesis and characterization of selenium nanoparticles and its anti-tumor potential on Dalton's lymphoma cells.
abstract: Carboxylic group-induced synthesis of selenium nanoparticles (SeNPs) was achieved using sodium selenosulphate as a precursor. The particles were stabilized and capped with 0.01% polyvinyl alcohol under ambient conditions. This is a simple and easy method of producing SeNPs in a size range from 35 to 105 nm. The synthesized SeNPs were purified by centrifugation at 11,500 × g for 20 min and characterized by UV-visible spectroscopy, FTIR spectroscopy, XRD, DSC and TEM. It was observed that the synthesized SeNPs showed differences in their absorption spectra, phase composition and crystal structure, thermodynamic behaviour, size and shape. Further, to confirm anti-tumour potential of the synthesized SeNPs induced by the carboxylic group of acetic acid, pyruvic acid and benzoic acid, cell viability assay, nuclear morphology testing and DNA fragmentation assay were carried out using Dalton's lymphoma (DL) cells. DL cells treated with the SeNPs showed reduced cell viability, altered nuclear morphology, typical apoptotic DNA ladder and apoptosis. Therefore, these SeNPs may have therapeutic relevance to treat this type of cancer.
authors.affiliation: Centre of Advance Study, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Centre of Advance Study, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India.
authors.forename: Munendra Singh
authors.initials: MS
authors.lastname: Tomar
authors.affiliation: Centre of Advance Study, Department of Zoology, Faculty of Science, Banaras Hindu University, Varanasi 221005, UP, India. Electronic address: acharya@bhu.ac.in.
authors.forename: Arbind
authors.initials: A
authors.lastname: Acharya
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D008223:Lymphoma / Q000188:drug therapy* / Q000473:pathology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D012643:Selenium / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D012643:Selenium
keywords: Apoptosis; Dalton's lymphoma; SeNPs