title: Full genome sequence analysis of a wild, non-MLV-related type 2 Hungarian PRRSV variant isolated in Europe.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is a widespread pathogen of pigs causing significant economic losses to the swine industry. The expanding diversity of PRRSV strains makes the diagnosis, control and eradication of the disease more and more difficult. In the present study, the authors report the full genome sequencing of a type 2 PRRSV strain isolated from piglet carcasses in Hungary. Next generation sequencing was used to determine the complete genome sequence of the isolate (PRRSV-2/Hungary/102/2012). Recombination analysis performed with the available full-length genome sequences showed no evidence of such event with other known PRRSV. Unique deletions and an insertion were found in the nsp2 region of PRRSV-2/Hungary/102/2012 when it was compared to the highly virulent VR2332 and JXA-1 prototype strains. The majority of amino acid alterations in GP4 and GP5 of the virus were in the known antigenic regions suggesting an important role for immunological pressure in PRRSV-2/Hungary/102/2012 evolution. Phylogenetic analysis revealed that it belongs to lineage 1 or 2 of type 2 PRRSV. Considering the lack of related PRRSV in Europe, except for a partial sequence from Slovakia, the ancestor of PRRSV-2/Hungary/102/2012 was most probably transported from North-America. It is the first documented type 2 PRRSV isolated in Europe that is not related to the Ingelvac MLV.
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Szent István University, István u. 2, H-1078 Budapest, Hungary. Electronic address: balka.gyula@aotk.szie.hu.
authors.forename: Gyula
authors.initials: G
authors.lastname: Balka
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
authors.forename: Xiong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Olasz
authors.affiliation: National Food Chain Safety Office Veterinary Diagnostic Directorate, Tábornok u. 2, H-1143 Budapest, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Bálint
authors.affiliation: Ceva-Phylaxia Veterinary Biologicals Co. Ltd., Szállás u. 5, H-1107 Budapest, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Kiss
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Bányai
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Szent István University, István u. 2, H-1078 Budapest, Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Rusvai
authors.affiliation: Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, ul. Nowoursynowska 159c, 02-776 Warsaw, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Stadejek
authors.affiliation: Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Marthaler
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Avenue, St. Paul, MN 55108, USA.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Murtaugh
authors.affiliation: Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Zádori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005060:Europe; D016679:Genome, Viral; D005838:Genotype; D059014:High-Throughput Nucleotide Sequencing; D006814:Hungary; D008969:Molecular Sequence Data; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Full genome; Next generation sequencing; PRRSV; Phylogeny; Swine; Type 2