title: The preparation of ethylenediamine-modified fluorescent carbon dots and their use in imaging of cells.
abstract: In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface-modified with ethylenediamine. The properties of as-prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet-visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti-(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as-prepared CDs in bioimaging and related fields.
authors.affiliation: Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.affiliation: ICU, Fengtian Hospital Affiliated to Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Siqi
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Experiment Center of Traditional Chinese Medicine Department, Shenyang Pharmaceutical University, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ge
authors.affiliation: Department of Chemistry, Shenyang Medical College, Shenyang, People's Republic of China.
authors.forename: Lili
authors.initials: L
authors.lastname: Sui
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000276:immunology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005029:Ethylenediamines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D055772:Green Chemistry Technology; D006801:Humans; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000737:chemistry / Q000494:pharmacology; D045663:Quantum Dots; D017550:Spectroscopy, Fourier Transform Infrared; D013274:Stomach Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D002272:Carcinoembryonic Antigen; D005029:Ethylenediamines; D005456:Fluorescent Dyes; D015335:Molecular Probes; C031234:ethylenediamine; D002244:Carbon
keywords: carbon dots; cell imaging; cytotoxicity; ethylenediamine