title: Elevated glucose-6-phosphate dehydrogenase expression in the cervical cancer cases is associated with the cancerigenic event of high-risk human papillomaviruses.
abstract: The most important etiologic agent in the pathogenesis of cervical cancers (CCs) is human papillomavirus (HPV), while the mechanisms underlying are still not well known. Glucose-6-phosphate dehydrogenase (G6PD) is reported to elevate in various tumor cells. However, no available references elucidated the correlation between the levels of G6PD and HPV-infected CC until now. In the present study, we explored the possible role of G6PD in the pathology of CC induced by HPV infection. Totally 48 patients with HPV + CC and another 63 healthy women enrolled in the clinical were employed in the present study. Overall, prevalence of cervical infection with high-risk-HPV (HR-HPV) type examined was HPV-16, followed by HPV-18. The expressions of G6PD in CC samples were also detected by immunohistochemistry (IHC), qRT-PCR, and Western blot. Regression analysis showed elevated G6PD level was positively correlated with the CC development in 30-40 aged patients with HR-HPV-16/18 infection. The HPV16 + Siha, HPV18 + Hela, and HPV-C33A cell lines were employed and transfected with G6PD deficient vectors developed in vitro. MTT and flow cytometry were also employed to determine the survival and apoptosis of CC cells after G6PD expressional inhibition. Our data revealed that G6PD down-regulation induced poor proliferation and more apoptosis of HPV18 + Hela cells, when compared with that of HPV16 + Siha and HPV-C33A cells. These findings suggest that G6PD expressions in the HR-HPV + human CC tissues and cell lines play an important role in tumor growth and proliferation.
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China.
authors.forename: Ya-Shan
authors.initials: YS
authors.lastname: Li
authors.affiliation: Experiment Center for Medical Science Research, Kunming Medical University, Kunming 650500, Yunnan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China.
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Chang
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China.
authors.forename: Guang-Cai
authors.initials: GC
authors.lastname: Liu
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming 650011, Yunnan, China.
authors.forename: Hong-Lan
authors.initials: HL
authors.lastname: Chen
authors.affiliation: Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan, China xiyang_neuro@126.com.
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Xiyang
mesh_terms: D000328:Adult; D052685:Alphapapillomavirus / Q000302:isolation & purification*; D017209:Apoptosis; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology; D012044:Regression Analysis; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000473:pathology* / Q000821:virology
chemical_list: D005954:Glucosephosphate Dehydrogenase
keywords: Glucose-6-phosphate dehydrogenase; apoptosis; cell proliferation; cervical cancer; high-risk human papillomavirus