title: Increased accumulation of protein-bound N(ε)-(carboxymethyl)lysine in tissues of healthy rats after chronic oral N(ε)-(carboxymethyl)lysine.
abstract: In recent years, chronic diseases related to advanced glycation end products (AGEs) have attracted more attention. Because diet is an important exogenous source of AGEs, this study aimed to investigate the effects of chronic oral administration of pure N(ε)-(carboxymethyl)lysine (CML) (a major AGE) at 60 mg kg(-1) per day on healthy Sprague-Dawley rats. After administration for 12 weeks, the levels of protein-bound CML were increased to 202 ± 17, 167 ± 47, 217 ± 44, 107 ± 4, 144 ± 23, and 33 ± 7 μg/g dry matter in the kidneys, heart, liver, lungs, spleen, and pancreas, respectively, in comparison with control values of 98 ± 1, 90 ± 15, 140 ± 42, 76 ± 18, 115 ± 15, and 30 ± 4 μg/g dry matter. The difference was significant (p < 0.05) for the kidneys, heart, liver, and lungs, whereas no significant increase was seen in the spleen and pancreas. Furthermore, serum blood urea nitrogen (BUN), creatinine (CREA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values increased significantly (p < 0.05), as evidence of impaired kidney and liver function. Additionally, the rats' fasting blood glucose (FBG) levels remained within the normal range, indicating that chronic intake of CML does not promote a rise in blood glucose. These results clearly indicate that a CML-rich diet might be a potential health risk in humans, particularly with respect to kidney and liver function.
authors.affiliation: State Key Laboratory of Food Science and Technology, and ‡Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University , 1800 Lihu Road, Wuxi Jiangsu 214122, People's Republic of China.
authors.forename: Mei
authors.initials: M
authors.lastname: Li
authors.forename: Maomao
authors.initials: M
authors.lastname: Zeng
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: He
authors.forename: Zongping
authors.initials: Z
authors.lastname: Zheng
authors.forename: Fang
authors.initials: F
authors.lastname: Qin
authors.forename: Guanjun
authors.initials: G
authors.lastname: Tao
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000633:toxicity; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism
chemical_list: D011506:Proteins; C048496:N(6)-carboxymethyllysine; D008239:Lysine
keywords: Nε-(carboxymethyl)lysine; advanced glycation end products; fasting blood glucose; serum markers; tissues