title: Molecular detection and characterization of BK and JC polyomaviruses in urine samples of renal transplant patients in Southern Brazil.
abstract: The human polyomaviruses JC (JCPyV) and BK (BKPyV) are widespread in the human population. Following the primary infection, virus reactivation may lead to nephropathy and graft rejection in renal transplant patients. This study was carried out to access the presence of BKPyV and JCPyV DNA in urine samples collected from renal transplant patients (n = 92) and healthy individuals (n = 88) in Porto Alegre, Rio Grande do Sul. The samples were submitted to a nested PCR. A significantly higher frequency (P < 0.001) of BKPyV was found in renal transplant patients (65.2%) in comparison to the control group (32.9%). JCPyV was detected equally in both groups. Phylogenetic analysis of both BKPyV and JCPyV amplicons demonstrates the presence of the BKPyV subtypes I and II, whereas for JCPyV, four different groups are found (1, 2, 3, and 4).
authors.affiliation: Virology Laboratory, Microbiology, Immunology, and Parasitology Department, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Comerlato
authors.forename: Fabrício Souza
authors.initials: FS
authors.lastname: Campos
authors.forename: Martha Trindade
authors.initials: MT
authors.lastname: Oliveira
authors.forename: Samuel Paulo
authors.initials: SP
authors.lastname: Cibulski
authors.forename: Lilian
authors.initials: L
authors.lastname: Corrêa
authors.forename: Marcos Iuri Roos
authors.initials: MI
authors.lastname: Kulmann
authors.forename: Thalita Souza
authors.initials: TS
authors.lastname: Arantes
authors.forename: Lucas Peretti
authors.initials: LP
authors.lastname: Hentges
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001739:BK Virus / Q000302:isolation & purification*; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D007223:Infant; D007577:JC Virus / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000453:epidemiology / Q000821:virology*; D015995:Prevalence; D066027:Transplant Recipients; D014412:Tumor Virus Infections / Q000453:epidemiology / Q000821:virology*; D014556:Urine / Q000821:virology*; D055815:Young Adult
keywords: BKPyV; JCPyV; nested PCR; viruria