title: COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae).
abstract: Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).
authors.affiliation: Department of Entomology,China Agricultural University,No. 2 Yuanmingyuan West Road,Beijing,China.
authors.forename: Z
authors.initials: Z
authors.lastname: Varadínová
authors.affiliation: Department of Entomology,China Agricultural University,No. 2 Yuanmingyuan West Road,Beijing,China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507,Prague,Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Kučerová
authors.affiliation: Department of Pest Control of Stored Products and Food Safety,Crop Research Institute,Drnovská 507,Prague,Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Stejskal
authors.affiliation: Department of Entomology and Plant Pathology,Oklahoma State University,127 Noble Research Center,Stillwater,Oklahoma,USA.
authors.forename: G
authors.initials: G
authors.lastname: Opit
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Academy of State Administration of Grain,No. 11 Baiwanzhuang Street,Beijing,China.
authors.forename: F J
authors.initials: FJ
authors.lastname: Li
authors.affiliation: Department of Entomology,China Agricultural University,No. 2 Yuanmingyuan West Road,Beijing,China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D001517:Coleoptera / Q000145:classification* / Q000235:genetics*; D058893:DNA Barcoding, Taxonomic; D017931:DNA Primers; D003576:Electron Transport Complex IV / Q000235:genetics; D005240:Feasibility Studies; D016266:Food Parasitology; D007814:Larva / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D003576:Electron Transport Complex IV
keywords: species-specific primer