title: Temperature-sensitive fluorescent organic nanoparticles with aggregation-induced emission for long-term cellular tracing.
abstract: Temperature-sensitive organic nanoparticles with AIE effect were assembled in water from tetraphenylethene-based poly(N-isopropylacrylamide) (TPE-PNIPAM), which was synthesized by ATRP using TPE derivative as initiator. The size and fluorescence of TPE-PNIPAM nanoparticles can be tuned by varying the temperature. These nanoparticles can be internalized readily by HeLa cells and can be used as long-term tracer in live cells to be retained for as long as seven passages.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology , and National Engineering Research Center for Nanomedicine, Wuhan, Hubei 430074, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wang
authors.forename: Tu-Ying
authors.initials: TY
authors.lastname: Yong
authors.forename: Jiangshan
authors.initials: J
authors.lastname: Wan
authors.forename: Zi-He
authors.initials: ZH
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Zhao
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Zhao
authors.forename: Lu
authors.initials: L
authors.lastname: Gan
authors.forename: Xiang-Liang
authors.initials: XL
authors.lastname: Yang
authors.forename: Hui-Bi
authors.initials: HB
authors.lastname: Xu
authors.forename: Chun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D058948:Cell Tracking / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008422:Materials Testing; D008856:Microscopy, Fluorescence / Q000379:methods*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D009930:Organic Chemicals / Q000737:chemistry*; D010316:Particle Size; D013347:Subcellular Fractions / Q000648:ultrastructure*; D013696:Temperature
chemical_list: D005456:Fluorescent Dyes; D009930:Organic Chemicals
keywords: aggregation-induced emission (AIE); fluorescent organic nanoparticles; long-term cellular tracing; poly(N-isopropylacrylamide) (PNIPAM); tetraphenylethene (TPE)