title: [Effect of traditional Chinese medicines with different properties on thermoregulation and temperature-sensitive transient receptor potentialion channel protein of rats with yeast-induced fever].
abstract: OBJECTIVE
  To compare the intervention effects of four traditional Chinese medicines (TCMs) with typical cold or hot property on body temperature and temperature-sensitive transient receptor potential ion channel proteins (TRPs) of rats with yeast-induced fever.
  
  
  METHODS
  The pyrexia model was induced by injecting yeast suspension subcutaneously. Totally 108 male SD rats were randomly divided into the normal group, the model group, the Rhei Radix et Rhizoma treated group, the Coptidis Rhizoma treated group, the Euodiae Fructus treated group, and the Alpiniae Officinarum Rhizoma treated group, with 18 rats in each group. At the 4 h, 8 h and 12 h after injection of yeast, the rats were sacrificed to collect their hypothalamus and dorsal root ganglion. The expressions of TRPV1 and TRPM8 were detected by immunohistochemistry and Western blot method.
  
  
  RESULTS
  Compared with the normal group, after injection of yeast, the temperature of rats in the model group notably increased, and reached the peak at 8 h (P < 0.01). The TRPV1 level in hypothalamus and dorsal root ganglia (DRG) of the model group significantly increased, whereas the TRPM8 level significantly reduced. Compared with the model group, the Rhei Radix et Rhizoma group and the Coptidis Rhizoma group showed significant decrease in the high body temperature of rats caused by yeast, down-regulation in the expression of TRPV1, and up-regulation in the expression of TRPM8 (P < 0.05 or P < 0.01). Euodiae Fructus and Alpiniae Officinarum Rhizoma had no significant effect on either temperature or TRPs of fever rats.
  
  
  CONCLUSIONS
  Rhei Radix et Rhizoma and Coptidis Rhizoma, both are TCMs with cold property, can reduce the temperature of fever rats induced by yeast, which may be related to their effective regulation of TRPV1 and TRPM8 in hypothalamus and DRG, while Euodiae Fructus and Alpiniae Officinarum Rhizoma had no relevant effect.
authors.forename: Hong-Ye
authors.initials: HY
authors.lastname: Wan
authors.forename: Xiang-Ying
authors.initials: XY
authors.lastname: Kong
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Li
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Zhu
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Su
authors.forename: Na
authors.initials: N
authors.lastname: Lin
mesh_terms: D000818:Animals; D058633:Antipyretics / Q000008:administration & dosage* / Q000737:chemistry; D001833:Body Temperature Regulation / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000737:chemistry*; D005334:Fever / Q000188:drug therapy* / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012441:Saccharomyces cerevisiae / Q000276:immunology*; D050053:TRPM Cation Channels / Q000235:genetics* / Q000276:immunology; D050916:TRPV Cation Channels / Q000235:genetics* / Q000276:immunology
chemical_list: D058633:Antipyretics; D004365:Drugs, Chinese Herbal; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; C498947:Trpm8 protein, rat; C493922:Trpv1 protein, rat