title: Competitive host-guest interaction between β-cyclodextrin polymer and pyrene-labeled probes for fluorescence analyses.
abstract: We developed a novel homogeneous fluorescence analysis based on a novel competitive host-guest interaction (CHGI) mechanism between β-cyclodextrin polymer (polyβ CD) and pyrene-labeled probe for biochemical assay. Pyrene labeling with oligonucleotide strands can be recruited and reside in lipophilic cavities of polyβ CD. This altered lipophilic microenvironment provides favored polarity for enhanced quantum efficiencies and extraordinarily increases the luminescence intensity of pyrene. However, with addition of complementary DNA, the pyrene-labeled probe formed double-strand DNA to hinder pyrene from entering the cavities of polyβ CD. The release of pyrene from polyβ CD, which are followed by fluorescence extinguishing, will provide the clear signal turn-off in the presence of target DNA. We also introduced Exodeoxyribonuclease I (Exo I) and Exodeoxyribonuclease III (Exo III) to improve the sensitivity of this system, and the following product of cleavage reaction, pyrene-nucleotide, could more easily host-guest interact with polyβ CD and emit stronger fluorescence than pyrene-labeled probe. In addition, the successful detection of adenosine is also demonstrated by using the similar sensing scheme. Although this scheme might be easily interfered by some biomolecules in the real test sample, it holds promising potential for detecting a broad range of other types of aptamer-binding chemicals and biomolecules.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University , Changsha, Hunan 410082, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Shan
authors.initials: S
authors.lastname: Sun
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Guo
authors.forename: Xiaohai
authors.initials: X
authors.lastname: Yang
authors.forename: Jin
authors.initials: J
authors.lastname: Huang
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Jianbo
authors.initials: J
authors.lastname: Liu
authors.forename: Leiliang
authors.initials: L
authors.lastname: He
mesh_terms: D000241:Adenosine / Q000032:analysis*; D001667:Binding, Competitive; D002482:Cellulose / Q000737:chemistry* / Q000378:metabolism; D003505:Cyclodextrins / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D057230:Limit of Detection; D011721:Pyrenes / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003505:Cyclodextrins; D005456:Fluorescent Dyes; D011721:Pyrenes; C041953:cyclodextrin polymer; D002482:Cellulose; D004247:DNA; C030984:pyrene; D000241:Adenosine