title: μ-Opioid receptor gene (OPRM1) polymorphism in patients with breast cancer.
abstract: Structure-dependent μ-opioid receptor (MOR) activity is an important element in cancer opioid analgesic effectiveness. It is widely accepted that guanine (G) substitution for adenine (A) at OPRM1 gene sequence position 118 changes receptor glycosylation pattern. This is associated with decreased binding ability in both exogenous and endogenous opioids, resulting in increased human pain resistance. The endogenous opioid system's function in body homeostasis maintenance is considered mainly regulatory, so its participation in breast tumor formation and progression is identified herein. We examine the association of the most frequent MOR (A118G) gene polymorphism on breast cancer risk in a Northeastern Polish population by PCR-RFLP comparison of A and G allele frequency at OPRM1 gene A118G polymorphic site in breast cancer-diagnosed patients with healthy control group frequencies. Our results highlight a strong association between G allele presence at μ-opioid receptor A118G and increased breast cancer incidence (OR = 3.3, 95 % CI 2.2-5.0, p < 0.0001) and female gender (OR = 2.0, 95 % CI 1.4-2.9, p = 0.0004). Consequently, OPRM1 G allele presence at that site is a highly significant risk factor in breast cancer development.
authors.affiliation: Department of Biochemistry, Faculty of Biology and Biotechnology, University of Warmia and Mazury, Oczapowskiego 1A Street, 10-719, Olsztyn, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Cieślińska
authors.forename: Edyta
authors.initials: E
authors.lastname: Sienkiewicz-Szłapka
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kostyra
authors.forename: Ewa
authors.initials: E
authors.lastname: Fiedorowicz
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Snarska
authors.forename: Konrad
authors.initials: K
authors.lastname: Wroński
authors.forename: Michał
authors.initials: M
authors.lastname: Tenderenda
authors.forename: Beata
authors.initials: B
authors.lastname: Jarmołowska
authors.forename: Michał
authors.initials: M
authors.lastname: Matysiewicz
mesh_terms: D000328:Adult; D000368:Aged; D000701:Analgesics, Opioid / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010146:Pain / Q000188:drug therapy / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017450:Receptors, Opioid, mu / Q000235:genetics*; D012307:Risk Factors
chemical_list: D000701:Analgesics, Opioid; C485764:OPRM1 protein, human; D017450:Receptors, Opioid, mu