title: Relationships between interleukin-12B and interleukin-10 gene polymorphisms and hepatitis C in Chinese Han hemodialysis patients.
abstract: OBJECTIVE
  Hepatitis C virus (HCV) commonly causes a chronic infection but portion of hemodialysis patients are able to resistant to HCV infection, even clear the virus naturally. Interleukin-10 (IL-10) and interleukin-12 (IL-12) are crucial regulators of the immune response to T-helper 1 (Th1) or T-helper 2 (Th2) categories and play a role in autoimmune and infectious diseases. This study was undertaken to investigate the possible association of genetic polymorphisms of 3' untranslated region (3'UTR) of the IL-12B (-1188) and IL-10 (-592 and -819) and hepatitis C in Chinese Han hemodialysis (HD) patients.
  
  
  METHODS
  The genotyping of IL-12B 3'UTR and IL-10 -592 and -819 were performed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method.
  
  
  RESULTS
  Compared with the IL-12B-AA genotype, CC and combined CC/AC genotypes were associated with a significant decreased risk of HCV infection in Chinese hemodialysis patients (p < 0.001). However, the IL-10-592 and -819 genotypes were not found significant difference both between the anti-HCV (+) and anti-HCV (-) patients (p > 0.05) and the persistent infection and viral clearance HD patients (p > 0.05).
  
  
  CONCLUSIONS
  The present study indicated that the polymorphisms of IL-12B 3'UTR might contribute to the susceptibility of HCV infection in Chinese HD population.
authors.affiliation: Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu , China and.
authors.forename: Bei
authors.initials: B
authors.lastname: Zhu
authors.forename: Cuiyu
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: He
authors.forename: Jianqing
authors.initials: J
authors.lastname: Wu
authors.forename: Rongbin
authors.initials: R
authors.lastname: Yu
authors.forename: Weihong
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease; D016174:Hepacivirus / Q000302:isolation & purification / Q000502:physiology; D006526:Hepatitis C / Q000150:complications / Q000235:genetics / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics*; D053762:Interleukin-12 Subunit p40 / Q000235:genetics*; D007676:Kidney Failure, Chronic / Q000150:complications / Q000628:therapy; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D006435:Renal Dialysis / Q000379:methods*
chemical_list: C508609:IL10 protein, human; C500879:IL12B protein, human; D053762:Interleukin-12 Subunit p40; D016753:Interleukin-10
keywords: Hepatitis C; IL-10 gene; Interleukin-12B gene; hemodialysis; polymorphisms