title: NADPH oxidase p22phox gene expression in ulcerative colitis.
abstract: OBJECTIVE
  Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, which catalyzes the formation of reactive oxygen species (ROS) in phagocytic cells, has five subunits: p67phox ("phox"refers to "phagocyte oxidase"), p47phox, p40phox, p22phox, and gp91phox (catalytic subunit). Oxidative stress resulting from the accumulation of ROS and/or defective removal of ROS by antioxidants has detrimental effects on cellular functions and may contribute to chronic inflammation. Disruption of the colonic mucosa due to the dysregulation of antioxidants or transformation enzymes may play a role in the pathogenesis of ulcerative colitis (UC) and influence the clinical features of this disease. In this study, we examined the expression of the gene encoding NADPH oxidase subunit p22phox cytochrome b-245, alphapolypeptidein the colonic mucosa to test its possible contribution in the pathogenesis of UC.
  
  
  METHODS
  Expression levels of mRNA in the inflamed and non-inflamed colonic mucosa (determined using colonoscopy)of 22 patients with UC and in the normal mucosa of 22 healthy controls were analyzed using real-time polymerase chain reaction.
  
  
  RESULTS
  Expression levels of mRNA were not significantly different between patients with inflamed and non-inflamed colonic mucosa (p>0.05) and betweenpatients with inflamed colonicmucosa and healthy controls (p>0.05).
  
  
  CONCLUSIONS
  Although our data suggest that expression of the gene encoding p22phox is not associated with chronic inflammation in patients with UC, other mechanisms can affect oxidative stress in these patients.
authors.affiliation: Department of Internal Medicine, Nizip State Hospital, Gaziantep, Turkey. drnesebulbul@yahoo.com.tr.
authors.forename: Neşe
authors.initials: N
authors.lastname: Bülbül
authors.forename: Elif
authors.initials: E
authors.lastname: Pala
authors.forename: Yusuf Ziya
authors.initials: YZ
authors.lastname: İğci
authors.forename: Bülent
authors.initials: B
authors.lastname: Göğebakan
authors.forename: Serdar
authors.initials: S
authors.lastname: Öztuzcu
authors.forename: Beyhan
authors.initials: B
authors.lastname: Cengiz
authors.forename: Recep
authors.initials: R
authors.lastname: Bayraktar
authors.forename: Muhammet Sait
authors.initials: MS
authors.lastname: Dağ
authors.forename: Musa
authors.initials: M
authors.lastname: Aydınlı
mesh_terms: D000328:Adult; D000368:Aged; D003093:Colitis, Ulcerative / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D055815:Young Adult
chemical_list: D019255:NADPH Oxidases; C086986:CYBA protein, human