title: Registered report: transcriptional amplification in tumor cells with elevated c-Myc.
abstract: The Lin et al. (2012), published in Cell in 2012. The experiments that will be replicated are those reported in Figures 3E and 3F. In these experiments, elevated levels of c-Myc in the P493-6 cell model of Burkitt's lymphoma results in an increase of the total level of RNA using UV/VIS spectrophotometry (Figure 3E; Lin et al., 2012) and on the mRNA levels/cell for a large set of genes using digital gene expression technology (Figure 3F; Lin et al., 2012). The Reproducibility Project: Cancer Biology is a collaboration between the eLife.
authors.affiliation: Bioexpression and Fermentation Facility, University of Georgia, Athens, Georgia.
authors.forename: David
authors.initials: D
authors.lastname: Blum
authors.affiliation: JHMI Deep Sequencing and Microarray Core Facility, Johns Hopkins University, Baltimore, Maryland.
authors.forename: Haiping
authors.initials: H
authors.lastname: Hao
authors.affiliation: University of Oxford, Oxford, United Kingdom.
authors.forename: Michael
authors.initials: M
authors.lastname: McCarthy
mesh_terms: D000704:Analysis of Variance; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D015203:Reproducibility of Results; D014158:Transcription, Genetic
chemical_list: D016271:Proto-Oncogene Proteins c-myc
keywords: Reproducibility Project: Cancer Biology; biochemistry; c-Myc; gene expression; human; methodology