title: Ichthyenterobacterium magnum gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from olive flounder (Paralichthys olivaceus).
abstract: A novel marine bacterium isolated from the intestine of cultured flounder (Paralichthys olivaceus) was studied by using a polyphasic taxonomic approach. The isolate was Gram-stain-negative, pleomorphic, aerobic, yellow and oxidase- and catalase-negative. Phylogenetic analysis of 16S rRNA gene sequences indicated that isolate Th6(T) formed a distinct branch within the family Flavobacteriaceae and showed 96.6% similarity to its closest relative, Bizionia hallyeonensis T-y7(T). The DNA G+C content was 29 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C(15 : 1) G, iso-C(15 : 0), iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and summed feature 3 (C(15 : 1)ω6c and/or C(16 : 1)ω7c). On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, the novel bacterium has been assigned to a novel species of a new genus for which the name Ichthyenterobacterium magnum gen. nov., sp. nov. is proposed. The type strain is Th6(T) ( = JCM 18636(T) = KCTC 32140(T)).
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Qismat
authors.initials: Q
authors.lastname: Shakeela
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Shehzad
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Kaihao
authors.initials: K
authors.lastname: Tang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005432:Flounder / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6