title: In vitro transport mechanism of psoralen in a human placental cell line (BeWo cells).
abstract: The mechanism by which psoralen is transported across the placenta was investigated in the BeWo human placental cell line derived from choriocarcinoma in a transwell assay system using liquid chromatography-mass spectrometry/mass spectrometry detection. Psoralen uptake by BeWo cells increased linearly over the concentration range of 0.01 µM to 100 µM (r (2) = 0.997) and was not saturable. Psoralen uptake by BeWo cells was not affected by temperature (4 °C, room temperature, and 37 °C; p > 0.05). Psoralen transport increased linearly over 180 min (r (2) = 0.988) with 3.08 ± 0.26 %, 5.47 ± 0.21 %, 7.54 ± 0.06 %, 9.40 ± 0.37 %, 11.49 ± 0.31 %, and 12.46 ± 0.61 % transferred from the apical chamber to the basolateral chamber in the transwell assays at 30, 60, 90, 120, 150, and 180 min, respectively. The rate of transport showed the same tendency, increasing linearly from 0.13 ± 0.01 pmol/s to 0.58 ± 0.03 pmol/s over the concentration range of 25 µM to 100 µM (r (2) = 0.989). The apparent permeability coefficient for psoralen (100 µM) was 5.62 ± 0.24 × 10(-6) cm/s and 5.53 ± 0.47 × 10(-6) cm/s before and after treatment with verapamil (100 µM), respectively (p > 0.05). The efflux value for psoralen was approximately 1. These data show that psoralen is well absorbed and crosses the placental barrier via passive diffusion in the BeWo cell line.
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Dianrong
authors.initials: D
authors.lastname: Song
authors.affiliation: Tianjin University of Traditional Chinese Medicine, Graduate School, Tianjin, P. R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Han
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, P. R. China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Du
mesh_terms: D001692:Biological Transport; D045744:Cell Line, Tumor; D005260:Female; D005363:Ficusin / Q000378:metabolism*; D006801:Humans; D010920:Placenta / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy
chemical_list: D005363:Ficusin