title: [The spiral ganglion degeneration and the expression of EFR3A in the cochlea of the deaf mice induced by co-administration of kanamycin and furosemide].
abstract: OBJECTIVE
  To investigate the spiral ganglion degeneration and the expression of EFR3A in the cochlea of the deaf mice induced by co-administration of kanamycin and furosemide.
  
  
  METHODS
  Eight weeks old C57BL/6J mice were administered with a single dose of kanamycin followed by furosemide, then fluorescent immunohistochemistry staining and transmission electron microscopy were applied to observe the SGNs' degeneration process and extent characteristics at 1, 5, 15, 30 and 60 days following treatment. We detected the expression of EFR3A during the degeneration of SGNs via fluorescent immunohistochemistry and western blotting.
  
  
  RESULTS
  Co-administration of kanamycin and furosemide quickly induced cochlear hair cell death in mice, and then caused progressive degeneration of SGNs. Our results showed that the abnormal morphology of SGNs occurredat day 5 following administration, and the number of SGNs began to decrease at day 15. Compared to the control group, it was found the remarkable increase of the EFR3A protein at the fifth day after co-administration, then decreased to the nearly normal at 15 days following treatment, and no further significant changes thereafter.
  
  
  CONCLUSIONS
  The changes of the EFR3A protein expression in the spiral ganglion of the cochlea in mice are coincidence with the time of the SGNs degeneration to happen, which imply that EFR3A may play an important role in the occurrence of the SGNs' degeneration in the cochlea in mice following hair cells loss.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Nie
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China. Email: mingliangxiang@163.com.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Xiang
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Chenling
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200092, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
mesh_terms: D000818:Animals; D003051:Cochlea / Q000378:metabolism*; D005665:Furosemide; D006198:Hair Cells, Auditory; D007612:Kanamycin; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D013136:Spiral Ganglion / Q000378:metabolism / Q000473:pathology*
chemical_list: C534332:EFR3 protein, S cerevisiae; C000604140:EFR3A protein, mouse; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D007612:Kanamycin; D005665:Furosemide