title: Control of Root Rot and Wilt Diseases of Roselle under Field Conditions.
abstract: Roselle (Hibiscus sabdariffa L.) is one of the most important medicinal crops in many parts of the world. In this study, the effects of microelements, antioxidants, and bioagents on Fusarium oxysporum, F. solani, and Macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. Preliminary studies were carried out in vitro in order to select the most effective members to be used in field control trials. Our results showed that microelements (copper and manganese), antioxidants (salicylic acid, ascorbic acid, and EDTA), a fungicide (Dithane M45) and biological control agents (Trichoderma harzianum and Bacillus subtilis) were significantly reduced the linear growth of the causal pathogens. Additionally, application of the previous microelements, antioxidants, a fungicide and biological control agents significantly reduced disease incidence of root rot and wilt diseases under field conditions. Copper, salicylic acid, and T. harzianum showed the best results in this respect. In conclusion, microelements, antioxidants, and biocontrol agents could be used as alternative strategies to fungicides for controlling root rot and wilt diseases in roselle.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, Gifu 501-1193, Japan. ; Department of Plant Pathology, Faculty of Agriculture, South Valley University, Qena 83523, Egypt.
authors.forename: Naglaa
authors.initials: N
authors.lastname: Hassan
authors.affiliation: Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt.
authors.forename: Mohsen Mohamed
authors.initials: MM
authors.lastname: Elsharkawy
authors.affiliation: Laboratory of Plant Pathology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: Laboratory of Plant Pathology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
authors.forename: Mitsuro
authors.initials: M
authors.lastname: Hyakumachi
keywords: Biological control; Hibiscus; Root rot disease; Wilt disease