title: Microfluidic toner-based analytical devices: disposable, lightweight, and portable platforms for point-of-care diagnostics with colorimetric detection.
abstract: This chapter describes the development of microfluidic toner-based analytical devices (μTADs) to perform clinical diagnostics using a scanner or cell-phone camera. μTADs have been produced in a platform composed of polyester and toner by the direct-printing technology (DPT) in a matter of minutes. This technology offers simplicity and versatility, and it does not require any sophisticated instrumentation. Toner-based devices integrate the current generation of disposable analytical devices along paper-based chips. The cost of one μTAD has been estimated to be lower than $0.10. In addition, these platforms are lightweight and portable thus enabling their use for point-of-care applications. In the last 5 years, great efforts have been dedicated to spread out the use of μTADs in bioassays. The current chapter reports the fabrication of printed microplates and integrated microfluidic toner-based devices for dengue diagnostics and rapid colorimetric assays with clinically relevant analytes including cholesterol, triglycerides, total proteins, and glucose. The use of μTADs associated with cell-phone camera may contribute to the health care, in special, to people housed in developing regions or with limited access to clinics and hospitals.
authors.affiliation: Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 131, Goiânia, GO, 74001-970, Brazil.
authors.forename: Karoliny Almeida
authors.initials: KA
authors.lastname: Oliveira
authors.forename: Fabrício Ribeiro
authors.initials: FR
authors.lastname: de Souza
authors.forename: Cristina Rodrigues
authors.initials: CR
authors.lastname: de Oliveira
authors.forename: Lucimeire Antonelli
authors.initials: LA
authors.lastname: da Silveira
authors.forename: Wendell Karlos Tomazelli
authors.initials: WK
authors.lastname: Coltro
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D001681:Biological Assay; D001786:Blood Glucose / Q000032:analysis; D040421:Cell Phone; D002784:Cholesterol / Q000097:blood; D003124:Colorimetry / Q000379:methods*; D003715:Dengue / Q000175:diagnosis / Q000821:virology; D003716:Dengue Virus / Q000302:isolation & purification*; D003906:Developing Countries; D004209:Disposable Equipment; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055616:Microtechnology; D010209:Paper; D019095:Point-of-Care Systems; D011091:Polyesters / Q000737:chemistry; D011327:Printing / Q000379:methods; D011506:Proteins / Q000032:analysis; D017216:Telemedicine / Q000295:instrumentation* / Q000379:methods; D014280:Triglycerides / Q000097:blood
chemical_list: D000914:Antibodies, Viral; D001786:Blood Glucose; D011091:Polyesters; D011506:Proteins; D014280:Triglycerides; D002784:Cholesterol