title: Long-lasting insecticide-treated house screens and targeted treatment of productive breeding-sites for dengue vector control in Acapulco, Mexico.
abstract: BACKGROUND
  Long-lasting insecticidal net screens (LLIS) fitted to domestic windows and doors in combination with targeted treatment (TT) of the most productive Aedes aegypti breeding sites were evaluated for their impact on dengue vector indices in a cluster-randomised trial in Mexico between 2011 and 2013.
  
  
  METHODS
  Sequentially over 2 years, LLIS and TT were deployed in 10 treatment clusters (100 houses/cluster) and followed up over 24 months. Cross-sectional surveys quantified infestations of adult mosquitoes, immature stages at baseline (pre-intervention) and in four post-intervention samples at 6-monthly intervals. Identical surveys were carried out in 10 control clusters that received no treatment.
  
  
  RESULTS
  LLIS clusters had significantly lower infestations compared to control clusters at 5 and 12 months after installation, as measured by adult (male and female) and pupal-based vector indices. After addition of TT to the intervention houses in intervention clusters, indices remained significantly lower in the treated clusters until 18 (immature and adult stage indices) and 24 months (adult indices only) post-intervention.
  
  
  CONCLUSIONS
  These safe, simple affordable vector control tools were well-accepted by study participants and are potentially suitable in many regions at risk from dengue worldwide.
authors.affiliation: Servicios de Salud de Yucatán, Gobierno del Estado de Yucatán, Mérida C.P. 97000, Mexico.
authors.forename: Azael
authors.initials: A
authors.lastname: Che-Mendoza
authors.affiliation: Universidad Autónoma de Yucatán, Mérida C.P. 97000, Mexico.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Guillermo-May
authors.affiliation: Universidad Autónoma de Yucatán, Mérida C.P. 97000, Mexico.
authors.forename: Josué
authors.initials: J
authors.lastname: Herrera-Bojórquez
authors.affiliation: Universidad Autónoma de Yucatán, Mérida C.P. 97000, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Barrera-Pérez
authors.affiliation: Servicios Estatales de Salud de Guerrero, Chilpancingo C.P. 39090, Mexico.
authors.forename: Felipe
authors.initials: F
authors.lastname: Dzul-Manzanilla
authors.affiliation: Servicios Estatales de Salud de Guerrero, Chilpancingo C.P. 39090, Mexico.
authors.forename: Cipriano
authors.initials: C
authors.lastname: Gutierrez-Castro
authors.affiliation: Centro Nacional de Programas Preventivos y Control de Enfermedades, Secretaria de Salud, Mexico.
authors.forename: Juan I
authors.initials: JI
authors.lastname: Arredondo-Jiménez
authors.affiliation: Centro Nacional de Programas Preventivos y Control de Enfermedades, Secretaria de Salud, Mexico.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sánchez-Tejeda
authors.affiliation: Department of Environmental Sciences, Emory University, Atlanta, GA, 30322, USA.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Vazquez-Prokopec
authors.affiliation: Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
authors.forename: Hilary
authors.initials: H
authors.lastname: Ranson
authors.affiliation: US Centers for Disease Control and Prevention, Atlanta, GA30329, USA.
authors.forename: Audrey
authors.initials: A
authors.lastname: Lenhart
authors.affiliation: Special Programme for Research and Training in Tropical Diseases (TDR), World Health Organization, 1211 Geneva 27, Switzerland.
authors.forename: Johannes
authors.initials: J
authors.lastname: Sommerfeld
authors.affiliation: Vector Biology Department, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: McCall
authors.affiliation: Special Programme for Research and Training in Tropical Diseases (TDR), World Health Organization, 1211 Geneva 27, Switzerland.
authors.forename: Axel
authors.initials: A
authors.lastname: Kroeger
authors.affiliation: Universidad Autónoma de Yucatán, Mérida C.P. 97000, Mexico pablo_manrique2000@hotmail.com.
authors.forename: Pablo
authors.initials: P
authors.lastname: Manrique-Saide
mesh_terms: D000330:Aedes / Q000254:growth & development*; D000818:Animals; D016000:Cluster Analysis; D003153:Community Health Services / Q000458:organization & administration*; D003715:Dengue / Q000517:prevention & control*; D003716:Dengue Virus / Q000302:isolation & purification / Q000472:pathogenicity*; D004197:Disease Reservoirs / Q000469:parasitology; D007722:Health Knowledge, Attitudes, Practice; D006801:Humans; D057148:Insecticide-Treated Bednets; D007306:Insecticides / Q000494:pharmacology*; D008800:Mexico / Q000453:epidemiology; D009032:Mosquito Control / Q000458:organization & administration*; D010342:Patient Acceptance of Health Care; D012621:Seasons; D018571:Sentinel Surveillance; D014504:Urban Health; D014881:Water Supply
chemical_list: D007306:Insecticides
keywords: Aedes aegypti; Control; Dengue; LLIS; Mexico; Targeted treatment