title: Cytoarchitecture of steroid dependent target tissues after testosterone administration compared to nandrolone decanoate in castrated rats in the aim of Hershberger bio test.
abstract: The objective was the cytoarchitecture evaluation of known steroid dependent target tissues after administering of testosterone, compared to action of its more active ester, nortestosterone (nandrolone decanoate) in castrated rat males in the aim of Hershberger bio test. Study was performed on 30 castrated male Wistar rats, aged between 35 and 39 days, in peripubertal period, divided into five groups. Androgen doses administration begun at the rats' age of 49 days. Animals were injected i.m., daily, for 10 consecutive days as follows: Aquatest (Balkan Pharmaceuticals Ltd., Moldova) testosterone aqueous solution: Testosterone I group (0.4 mg/animal); Testosterone II (0.8 mg/animal); (Deca-Durabolin, Balkan Pharmaceuticals); nandrolone decanoate oily solution: Nortestosterone I (1.5 mg/kg body weight); Nortestosterone II (7.5 mg/kg body weight) and Control (White sesame oil, Manicos, Romania, 0.1 mL/animal). Gonadectomy (GDX) induced modifications of target tissues wet weight accompanied by important modifications in cytoarchitecture. Changes following exogenous administration of testosterone and nortestosterone decanoate were found in: liver (granular dystrophy, mega-mitochondria, tubular intumescences), prostate (increasing of the structural elements), seminal vesicles (hyalinosis, thickening of cell walls and the hyaline presence), levator ani-bulbo-cavernosus muscle (muscle fibbers dilacerations), bulbourethral glands (muscular fibbers rarefaction by fluid accumulation) demonstrating the disruptor activity especially for overdosed nandrolone decanoate.
authors.affiliation: Department of Pharmacology and Pharmacy, Faculty of Veterinary Medicine, Banat's University of Agriculture and Veterinary Medicine, Timisoara, Romania; rtcristina@yahoo.com.
authors.forename: Romeo Teodor
authors.initials: RT
authors.lastname: Cristina
authors.forename: Flavia
authors.initials: F
authors.lastname: Hanganu
authors.forename: Diana
authors.initials: D
authors.lastname: Brezovan
authors.forename: Eugenia
authors.initials: E
authors.lastname: Dumitrescu
authors.forename: Florin
authors.initials: F
authors.lastname: Muselin
authors.forename: Viorica
authors.initials: V
authors.lastname: Chiurciu
authors.forename: Adrian Constantin
authors.initials: AC
authors.lastname: Stancu
authors.forename: Marius Cristian
authors.initials: MC
authors.lastname: Pentea
authors.forename: Andrei Gheorghe Marius
authors.initials: AG
authors.lastname: Motoc
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002369:Castration; D008297:Male; D009277:Nandrolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077603:Nandrolone Decanoate; D009929:Organ Size / Q000187:drug effects; D009928:Organ Specificity / Q000187:drug effects*; D017208:Rats, Wistar; D013739:Testosterone / Q000008:administration & dosage* / Q000494:pharmacology*
chemical_list: D013739:Testosterone; D009277:Nandrolone; D000077603:Nandrolone Decanoate