title: Plant phosphorus acquisition in a common mycorrhizal network: regulation of phosphate transporter genes of the Pht1 family in sorghum and flax.
abstract: In a preceding microcosm study, we found huge differences in phosphorus (P) acquisition in sorghum (Sorghum bicolor) and flax (Linum usitatissimum) sharing a common mycorrhizal network (CMN). Is the transcriptional regulation of arbuscular mycorrhizal (AM)-induced inorganic orthophosphate (Pi) transporters responsible for these differences? We characterized and analyzed the expression of Pi transporters of the Pht1 family in both plant species, and identified two new AM-inducible Pi transporters in flax. Mycorrhizal Pi acquisition was strongly affected by the combination of plant and AM fungal species. A corresponding change in the expression of two AM-inducible Pht1 transporters was noticed in both plants (SbPT9, SbPT10, LuPT5 and LuPT8), but the effect was very weak. Overall, the expression level of these genes did not explain why flax took up more Pi from the CMN than did sorghum. The post-transcriptional regulation of the transporters and their biochemical properties may be more important for their function than the fine-tuning of their gene expression.
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.|Plant-Soil-Interactions, Agroscope Institute of Sustainability Science, Reckenholzstrasse 191, CH-8046, Zürich, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Walder
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
authors.forename: Daphnée
authors.initials: D
authors.lastname: Brulé
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
authors.forename: Sally
authors.initials: S
authors.lastname: Koegel
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
authors.forename: Andres
authors.initials: A
authors.lastname: Wiemken
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Boller
authors.affiliation: Department of Environmental Sciences, Botany, Zurich-Basel Plant Science Center, University of Basel, Hebelstrasse 1, CH-4056, Basel, Switzerland.
authors.forename: Pierre-Emmanuel
authors.initials: PE
authors.lastname: Courty
mesh_terms: D019597:Flax / Q000235:genetics* / Q000382:microbiology*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D038821:Mycorrhizae / Q000502:physiology*; D009928:Organ Specificity / Q000235:genetics; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000382:microbiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D045868:Sorghum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D028061:Phosphate Transport Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D010758:Phosphorus
keywords: arbuscular mycorrhizal symbiosis; common mycorrhizal network (CMN); flax (Linum usitatissimum); gene expression; phosphate Pht1 transporters; sorghum (Sorghum bicolor)