title: Effect of combination of nerve fragments with nerve growth factor in autologous epineurium small gap coaptation on peripheral nerve injury repair.
abstract: The aim of this study was to explore the effect of the combination of nerve fragments with nerve growth factor (NGF) on the repair of peripheral nerve injury through autologous epineurium small gap coaptation. A total of 150 male Sprague-Dawley rats weighing 200-250 g were divided into five groups randomly with 30 rats per group, including the following: a control group that was subjected to traditional end-to-end neuroanastomosis; an autologous epineurium small gap group that received autologous epineurium small gap coaptation suture; a nerve fragments group in which nerve fragments were added to the small gap; an NGF group in which NGF was added to the small gap; and an NGF combined with nerve fragments group in which both NGF and nerve fragments were added to the small gap. All groups were examined at 4, 6, and 8 weeks after the operation, respectively; furthermore, electroneurophysiological detection and histological observation were performed at 8 weeks. Autonomic activities and root ulcers recovered sooner in rats in the NGF combined with nerve fragments group than the other groups. Moreover, the numbers of regenerated nerve fibers were greater and nerve conduction velocity was faster in the NGF combined with nerve fragments group than the other groups. Therefore, the combination of NGF with nerve fragments plays a significant role in the repair of peripheral nerve injury through autologous epineurium small gap coaptation. Therefore, compared with the other four methods, the combination of nerve fragments with NGF on the repair of peripheral nerve injury through autologous epineurium small gap coaptation has a better effect.
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China. bofengdoc@126.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Feng
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: He
authors.initials: H
authors.lastname: Hu
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Youming
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Niu
authors.affiliation: Department of Osteology, The Third Clinical Medical College of Inner Mongolia Medical University, No. 20 Shaoxian Road, Kudunlun District, Baotou, 014010, Inner Mongolia, China.
authors.forename: Ligong
authors.initials: L
authors.lastname: Lin
mesh_terms: D000818:Animals; D003131:Combined Modality Therapy / Q000295:instrumentation / Q000379:methods; D004343:Drug Implants / Q000008:administration & dosage; D004867:Equipment Design; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D008297:Male; D009414:Nerve Growth Factors / Q000008:administration & dosage*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D059348:Peripheral Nerve Injuries / Q000175:diagnosis / Q000503:physiopathology* / Q000628:therapy*; D010525:Peripheral Nerves / Q000637:transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000637:transplantation*; D013536:Suture Techniques / Q000295:instrumentation; D054457:Tissue Scaffolds; D014182:Transplantation, Autologous / Q000295:instrumentation / Q000379:methods; D016896:Treatment Outcome
chemical_list: D004343:Drug Implants; D009414:Nerve Growth Factors
keywords: Nerve debris; Nerve growth factor; Nerve repair; Small gap bridging