title: Polyclonality among clinical strains of non-pigmented rapidly growing mycobacteria: phenotypic and genotypic differences and their potential implications.
abstract: To investigate the potential implications (especially the implications in clinical significance and antimicrobial susceptibility) of polyclonality among rapidly growing mycobacteria, we performed random amplified polymorphic DNA analysis in 64 clinical isolates of which the clinical significance was established. Phenotypic characteristics (antimicrobial susceptibility test, colony morphology and growth rate) of each clone were studied. Polyclonality was detected in 13 of the isolates (20.3%). There was a relationship between monoclonality and clinical significance (p 0.0096). Monoclonal and polyclonal isolates showed different behaviour in antimicrobial susceptibility. There was a strong relationship between monoclonality and those species that are more pathogenic for humans, and also with clinical significance of the isolates.
authors.affiliation: Department of Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: García-Pedrazuela
authors.affiliation: Department of Microbiology, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Hospitalet de Llobregat, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Frutos
authors.affiliation: Department of Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Muñoz-Egea
authors.affiliation: Department of Microbiology, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Hospitalet de Llobregat, Spain; GEIM (Mycobacterial Infections Study Group), Spain.
authors.forename: F
authors.initials: F
authors.lastname: Alcaide
authors.affiliation: Department of Microbiology, Hospital Vall D'Hebron, Barcelona, Spain; GEIM (Mycobacterial Infections Study Group), Spain.
authors.forename: T
authors.initials: T
authors.lastname: Tórtola
authors.affiliation: Department of Microbiology, Hospital Clinico de Zaragoza, Zaragoza, Spain; GEIM (Mycobacterial Infections Study Group), Spain.
authors.forename: A
authors.initials: A
authors.lastname: Vitoria
authors.affiliation: Department of Genetics and Microbiology, UAB, Bellaterra, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Cortés
authors.affiliation: Department of Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain; GEIM (Mycobacterial Infections Study Group), Spain. Electronic address: jestebanmoreno@gmail.com.
authors.forename: J
authors.initials: J
authors.lastname: Esteban
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D058889:Molecular Typing; D009165:Mycobacterium Infections, Nontuberculous / Q000453:epidemiology* / Q000382:microbiology*; D009170:Nontuberculous Mycobacteria / Q000145:classification* / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification*; D010641:Phenotype; D010860:Pigments, Biological / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D000900:Anti-Bacterial Agents; D010860:Pigments, Biological
keywords: Genotypic; polyclonality; random amplified polymorphic DNA; rapidly growing mycobacteria; susceptibility