title: Da-bu-yin-wan and qian-zheng-san to neuroprotect the mouse model of Parkinson's disease.
abstract: Da-Bu-Yin-Wan (DBYW) and Qian-Zheng-San (QZS), two classic traditional Chinese medicinal formulas, were clinically employed to treat Parkinson's disease (PD). Our previous studies demonstrated neuroprotective effects of them on mitochondrial function in PD mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The purpose of this research was to investigate their possible mechanisms in the light of mitochondrial ATP-sensitive potassium (mitoKATP) channels. The neuroprotective effect of DBYW and QZS on dopamine (DA) neurons in substantia nigra (SN) in the MPTP-induced PD mice was investigated by behavioral test (pole test) and immunohistochemistry. Adenosine triphosphate (ATP) level in the midbrain tissue was detected by firefly luciferase method. MitoKATP channel subunits SUR1 and Kir6.2 mRNA and protein expressions were tested by real-time PCR (RT-PCR) and Western blot. It was observed that DBYW and/or QZS served to ameliorate MPTP-induced behavioral impairment and prevent the loss of substantia nigra dopamine neurons, as well as increase ATP level in the midbrain tissue and downregulate SUR1 expression at mRNA and protein levels with no marked influence on Kir6.2. We concluded that DBYW and QZS exhibit neuroprotective effects probably through the regulation of ATP level and mitoKATP channel subunit expressions.
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Gong
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Hong-Mei
authors.initials: HM
authors.lastname: Sun
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center of Scientific Research, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Shu-Jing
authors.initials: SJ
authors.lastname: Zhang
authors.affiliation: Center of Scientific Research, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Yu-Shan
authors.initials: YS
authors.lastname: Gao
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.affiliation: Center of Scientific Research, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Jing-Hong
authors.initials: JH
authors.lastname: Hu
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Gai
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Guo
authors.affiliation: Center of Scientific Research, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Anatomy, School of Preclinical Medicine, Beijing University of Chinese Medicine, No. 11 N. 3rd Ring Eastern Road, Beijing 100029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ma