title: Dark fermentation of complex waste biomass for biohydrogen production by pretreated thermophilic anaerobic digestate.
abstract: The Biohydrogen Potential (BHP) of six different types of waste biomass typical for the Campania Region (Italy) was investigated. Anaerobic sludge pre-treated with the specific methanogenic inhibitor sodium 2-bromoethanesulfonic acid (BESA) was used as seed inoculum. The BESA pre-treatment yielded the highest BHP in BHP tests carried out with pre-treated anaerobic sludge using potato and pumpkin waste as the substrates, in comparison with aeration or heat shock pre-treatment. The BHP tests carried out with different complex waste biomass showed average BHP values in a decreasing order from potato and pumpkin wastes (171.1 ± 7.3 ml H2/g VS) to buffalo manure (135.6 ± 4.1 ml H2/g VS), dried blood (slaughter house waste, 87.6 ± 4.1 ml H2/g VS), fennel waste (58.1 ± 29.8 ml H2/g VS), olive pomace (54.9 ± 5.4 ml H2/g VS) and olive mill wastewater (46.0 ± 15.6 ml H2/g VS). The digestate was analyzed for major soluble metabolites to elucidate the different biochemical pathways in the BHP tests. These showed the H2 was produced via mixed type fermentation pathways.
authors.affiliation: Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Di Biasio 43, 03043 Cassino, FR, Italy; Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Anish
authors.initials: A
authors.lastname: Ghimire
authors.affiliation: Department of Mathematics and Applications Renato Caccioppoli, University of Naples Federico II, via Cintia, Monte S. Angelo, I-80126 Naples, Italy. Electronic address: luigi.frunzo@unina.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Frunzo
authors.affiliation: Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Di Biasio 43, 03043 Cassino, FR, Italy; Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Ludovico
authors.initials: L
authors.lastname: Pontoni
authors.affiliation: Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: d'Antonio
authors.affiliation: UNESCO-IHE Institute for Water Education, Westvest 7, 2611 AX Delft, The Netherlands.
authors.forename: Piet N L
authors.initials: PN
authors.lastname: Lens
authors.affiliation: Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Di Biasio 43, 03043 Cassino, FR, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Esposito
authors.affiliation: Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Pirozzi
mesh_terms: D000383:Agriculture; D017738:Alkanesulfonic Acids / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D052918:Environmental Restoration and Remediation / Q000379:methods*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D007220:Industrial Waste / Q000032:analysis*; D007558:Italy
chemical_list: D017738:Alkanesulfonic Acids; D007220:Industrial Waste; C018853:2-bromoethanesulfonic acid; D006859:Hydrogen
keywords: Biofuels; Biohydrogen; Dark fermentation; Waste biomass; Waste valorization