title: Use of plant colonizing bacteria as chassis for transfer of N₂-fixation to cereals.
abstract: Engineering cereal crops that are self-supported by nitrogen fixation has been a dream since the 1970s when nitrogenase was transferred from Klebsiella pneumoniae to Escherichia coli. A renewed interest in this area has generated several new approaches with the common aim of transferring nitrogen fixation to cereal crops. Advances in synthetic biology have afforded the tools to rationally engineer microorganisms with traits of interest. Nitrogenase biosynthesis has been a recent target for the application of new synthetic engineering tools. Early successes in this area suggest that the transfer of nitrogenase and other supporting traits to microorganisms that already closely associate with cereal crops is a logical approach to deliver nitrogen to cereal crops.
authors.affiliation: Department of Plant Sciences, Oxford University, Oxford OX1 3RB, United Kingdom.
authors.forename: Barney A
authors.initials: BA
authors.lastname: Geddes
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Min-Hyung
authors.initials: MH
authors.lastname: Ryu
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA.
authors.forename: Florence
authors.initials: F
authors.lastname: Mus
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA.
authors.forename: Amaya
authors.initials: A
authors.lastname: Garcia Costas
authors.affiliation: Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Peters
authors.affiliation: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Voigt
authors.affiliation: Department of Plant Sciences, Oxford University, Oxford OX1 3RB, United Kingdom. Electronic address: philip.poole@plants.ox.ac.uk.
authors.forename: Philip
authors.initials: P
authors.lastname: Poole
mesh_terms: D018556:Crops, Agricultural / Q000378:metabolism; D002523:Edible Grain / Q000378:metabolism* / Q000382:microbiology*; D056426:Genetic Loci; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D058615:Synthetic Biology
chemical_list: D009584:Nitrogen