title: MiR-204-5p suppresses cell proliferation by inhibiting IGFBP5 in papillary thyroid carcinoma.
abstract: microRNAs (miRNAs) are frequently dysregulated in human malignancies. It was recently shown that miR-204-5p is downregulated in papillary thyroid carcinoma (PTC); however, the functional significance of this observation is not known. This study investigated the role of miR-204-5p in PTC. Overexpressing miR-204-5p suppressed PTC cell proliferation and induced cell cycle arrest and apoptosis. The results of a luciferase reporter assay showed that miR-204-5p can directly bind to the 3' untranslated region (UTR) of insulin-like growth factor-binding protein 5 (IGFBP5) mRNA, and IGFBP5 overexpression partially reversed the growth-inhibitory effects of miR-204-5p. These results indicate that miR-204-5p acts as a tumor suppressor in PTC by regulating IGFBP5 expression and that miR-204-5p can potentially serve as an antitumorigenic agent in the treatment of PTC.
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Lianyong
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Jingnan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Xiangqi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shi
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xi
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Jichen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Endocrine, Shanghai Pudong Gongli Hospital, Shanghai 200135, China. Electronic address: mjgugonglihos@yeah.net.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Gu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D002291:Carcinoma, Papillary; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018975:Insulin-Like Growth Factor Binding Protein 5 / Q000235:genetics*; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D020413:3' Untranslated Regions; D018975:Insulin-Like Growth Factor Binding Protein 5; C549404:MIRN204 microRNA, human; D035683:MicroRNAs
keywords: IGFBP5; Papillary thyroid carcinoma; Proliferation; miR-204-5p