title: Effects of methionine supplementation on the expression of oxidative stress-related genes in acute heat stress-exposed broilers.
abstract: The aim of the present study was to evaluate the effects of heat stress (HS) and methionine supplementation on the markers of stress and on the gene expression levels of uncoupling proteins (UCP), betaine-homocysteine methyltransferase (BHMT), cystathionine β-synthase (CBS), glutathione synthetase (GSS) and glutathione peroxidase 7 (GPx7). Broilers from 1 to 21 d and from 22 to 42 d of age were divided into three treatment groups related to methionine supplementation: without methionine supplementation (MD); recommended level of methionine supplementation (DL1); excess methionine supplementation (DL2). The broilers were either kept at a comfortable thermal temperature or exposed to HS (38°C for 24 h). During the starter period, we observed the effects of the interaction between diet and environment on the gene expression levels of UCP, BHMT and GSS. Higher gene expression levels of UCP and BHMT were observed in broilers that were maintained at thermal comfort conditions and received the MD diet. HS broilers fed the DL1 and DL2 diets had the highest expression level of GSS. The expression levels of the CBS and GPx7 genes were influenced by both the environment and methionine supplementation. During the grower period, the gene expression levels of BHMT, CBS, GSS and GPx7 were affected by the diet × environment interaction. A higher expression level of BHMT was observed in broilers maintained at thermal comfort conditions and on the MD diet. HS induced higher expression levels of CBS, GSS and GPx7 in broilers that received the DL1 and DL2 diets. The present results suggest that under HS conditions, methionine supplementation could mitigate the effects of stress, since methionine contributed to the increased expression levels of genes related to antioxidant activity.
authors.affiliation: Department of Animal Science,Universidade Estadual de Maringá (UEM),Avenida Colombo,5790Maringá,Paraná,Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Del Vesco
authors.affiliation: Department of Animal Science,Universidade Estadual de Maringá (UEM),Avenida Colombo,5790Maringá,Paraná,Brazil.
authors.forename: Eliane
authors.initials: E
authors.lastname: Gasparino
authors.affiliation: Department of Animal Science,Universidade Estadual de Maringá (UEM),Avenida Colombo,5790Maringá,Paraná,Brazil.
authors.forename: Daiane de Oliveira
authors.initials: Dde O
authors.lastname: Grieser
authors.affiliation: Department of Animal Science,Universidade Estadual de Maringá (UEM),Avenida Colombo,5790Maringá,Paraná,Brazil.
authors.forename: Vittor
authors.initials: V
authors.lastname: Zancanela
authors.affiliation: Department of Genetics,Universidade Federal Rural do Rio de Janeiro (UFRRJ), Seropédica,Rio de Janeiro,Brazil.
authors.forename: Maria Amélia Menck
authors.initials: MA
authors.lastname: Soares
authors.affiliation: Evonik-Degussa,Guarulhos,SP07222-000,Brazil.
authors.forename: Adhemar Rodrigues de Oliveira
authors.initials: AR
authors.lastname: Neto
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D050940:Betaine-Homocysteine S-Methyltransferase / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001715:Bird Diseases / Q000178:diet therapy / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002645:Chickens; D004032:Diet / Q000662:veterinary*; D002149:Energy Intake; D018507:Gene Expression Regulation, Developmental; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D005981:Glutathione Synthase / Q000235:genetics / Q000378:metabolism; D018882:Heat Stress Disorders / Q000178:diet therapy / Q000378:metabolism / Q000517:prevention & control / Q000662:veterinary*; D006710:Homocysteine / Q000097:blood; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000008:administration & dosage / Q000627:therapeutic use*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D010369:Pectoralis Muscles / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D000071256:Uncoupling Protein 1; D015430:Weight Gain
chemical_list: D000975:Antioxidants; D030161:Avian Proteins; D015415:Biomarkers; D007473:Ion Channels; D007527:Isoenzymes; D024101:Mitochondrial Proteins; D000071256:Uncoupling Protein 1; D006710:Homocysteine; D008715:Methionine; D005979:Glutathione Peroxidase; D050940:Betaine-Homocysteine S-Methyltransferase; D005981:Glutathione Synthase
keywords: Oxidative stress