title: Collagen XVII expression correlates with the invasion and metastasis of colorectal cancer.
abstract: Collagen XVII has a well-established role as an adhesion molecule and a cell surface receptor located in the type I hemidesmosome of stratified epithelia. Its ectodomain is constitutively shed from the cell surface and suggested to regulate the adhesion, migration, and signaling of cutaneous epithelial cells. Collagen XVII was not previously thought to be expressed by colon epithelial cells. Immunohistochemical analysis of tissue microarray samples of 141 cases of colorectal carcinoma showed that collagen XVII is expressed in normal human colonic mucosa and colorectal carcinoma. In colorectal carcinoma, increased collagen XVII expression was significantly associated with higher TNM stage. It also correlated with infiltrative growth pattern and tumor budding as well as lymph node and distant metastasis. Increased collagen XVII expression was associated with decreased disease-free and cancer-specific survival. Immunofluorescence staining of collagen XVII and its well-known binding partner laminin γ2 chain demonstrated a partial colocalization in normal and tumor tissue. In vitro, the overexpression of murine collagen XVII promoted the invasion of CaCo-2 colon carcinoma cells through Matrigel (BD Biosciences; Bedford, MA). To conclude, this study reports for the first time the expression of collagen XVII in colon epithelium and the association of increased collagen XVII immunoexpression with poor outcome in colorectal carcinoma.
authors.affiliation: Department of Dermatology and Oulu Center for Cell-Matrix Research, University of Oulu, FIN-90220, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland.
authors.forename: Jyri M
authors.initials: JM
authors.lastname: Moilanen
authors.affiliation: Department of Dermatology and Oulu Center for Cell-Matrix Research, University of Oulu, FIN-90220, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland.
authors.forename: Nina
authors.initials: N
authors.lastname: Kokkonen
authors.affiliation: Department of Dermatology, University Medical Center Freiburg and Freiburg Institute of Advanced Studies, University of Freiburg, D-79104, Freiburg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Löffek
authors.affiliation: Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland; Department of Pathology, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland.
authors.forename: Juha P
authors.initials: JP
authors.lastname: Väyrynen
authors.affiliation: Department of Pathology, Kainuu Central Hospital, FIN-87140, Kajaani, Finland.
authors.forename: Erkki
authors.initials: E
authors.lastname: Syväniemi
authors.affiliation: Department of Dermatology and Oulu Center for Cell-Matrix Research, University of Oulu, FIN-90220, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland.
authors.forename: Tiina
authors.initials: T
authors.lastname: Hurskainen
authors.affiliation: Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland; Department of Pathology, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland.
authors.forename: Markus
authors.initials: M
authors.lastname: Mäkinen
authors.affiliation: Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland; Department of Surgery, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland.
authors.forename: Kai
authors.initials: K
authors.lastname: Klintrup
authors.affiliation: Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland; Department of Surgery, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland.
authors.forename: Jyrki
authors.initials: J
authors.lastname: Mäkelä
authors.affiliation: Department of Pathology, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland; Biocenter Oulu, FIN-90220, Oulu, Finland.
authors.forename: Raija
authors.initials: R
authors.lastname: Sormunen
authors.affiliation: Department of Dermatology, University Medical Center Freiburg and Freiburg Institute of Advanced Studies, University of Freiburg, D-79104, Freiburg, Germany.
authors.forename: Leena
authors.initials: L
authors.lastname: Bruckner-Tuderman
authors.affiliation: Department of Pathology, University of Oulu and Oulu University Hospital, FIN-90220, Oulu, Finland.
authors.forename: Helena
authors.initials: H
authors.lastname: Autio-Harmainen
authors.affiliation: Department of Dermatology and Oulu Center for Cell-Matrix Research, University of Oulu, FIN-90220, Oulu, Finland; Medical Research Center Oulu, Oulu University Hospital and University of Oulu, FIN-90220, Oulu, Finland. Electronic address: kaisa.tasanen@oulu.fi.
authors.forename: Kaisa
authors.initials: K
authors.lastname: Tasanen
mesh_terms: D000368:Aged; D001324:Autoantigens / Q000032:analysis*; D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis*; D018938:Caco-2 Cells / Q000737:chemistry / Q000473:pathology; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D018572:Disease-Free Survival; D005260:Female; D005455:Fluorescent Antibody Technique; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology*; D009367:Neoplasm Staging; D018307:Neoplasms, Squamous Cell / Q000737:chemistry* / Q000401:mortality / Q000473:pathology / Q000556:secondary* / Q000628:therapy; D024041:Non-Fibrillar Collagens / Q000032:analysis*; D012372:ROC Curve; D016019:Survival Analysis; D016896:Treatment Outcome; D000097805:Collagen Type XVII
chemical_list: D001324:Autoantigens; D014408:Biomarkers, Tumor; D024041:Non-Fibrillar Collagens; D000097805:Collagen Type XVII
keywords: Adhesion; BP180; Basement membrane; Collagen XVII; Colorectal cancer; Hemidesmosome; Invasion