title: miR-92a is upregulated in cervical cancer and promotes cell proliferation and invasion by targeting FBXW7.
abstract: MicroRNAs (miRNAs) are involved in the cervical carcinogenesis and progression. In this study, we investigated the role of miR-92a in progression and invasion of cervical cancer. MiR-92a was significantly upregulated in cervical cancer tissues and cell lines. Overexpression of miR-92a led to remarkably enhanced proliferation by promoting cell cycle transition from G1 to S phase and significantly enhanced invasion of cervical cancer cells, while its knockdown significantly reversed these cellular events. Bioinformatics analysis suggested F-box and WD repeat domain-containing 7 (FBXW7) as a novel target of miR-92a, and miR-92a suppressed the expression level of FBXW7 mRNA by direct binding to its 3'-untranslated region (3'UTR). Expression of miR-92a was negatively correlated with FBXW7 in cervical cancer tissues. Furthermore, Silencing of FBXW7 counteracted the effects of miR-92a suppression, while its overexpression reversed oncogenic effects of miR-92a. Together, these findings indicate that miR-92a acts as an onco-miRNA and may contribute to the progression and invasion of cervical cancer, suggesting miR-92a as a potential novel diagnostic and therapeutic target of cervical cancer.
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Chuanyi
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China. Electronic address: lfshen2008@163.com.
authors.forename: Liangfang
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Radiation Oncology, Yueyang Second People's Hospital, Yueyang 414000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Radiation Oncology, Yueyang Second People's Hospital, Yueyang 414000, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology, Yueyang Second People's Hospital, Yueyang 414000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Yueyang Second People's Hospital, Yueyang 414000, China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Yu
mesh_terms: D020413:3' Untranslated Regions; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D044783:F-Box Proteins / Q000235:genetics / Q000378:metabolism*; D000073979:F-Box-WD Repeat-Containing Protein 7; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells / Q000473:pathology; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D044767:Ubiquitin-Protein Ligases
keywords: Cervical cancer; FBXW7; Invasion; Proliferation; miR-92a