title: Casein kinase 1 promotes initiation of clathrin-mediated endocytosis.
abstract: In budding yeast, over 60 proteins functioning in at least five modules are recruited to endocytic sites with predictable order and timing. However, how sites of clathrin-mediated endocytosis are initiated and stabilized is not well understood. Here, the casein kinase 1 (CK1) Hrr25 is shown to be an endocytic protein and to be among the earliest proteins to appear at endocytic sites. Hrr25 absence or overexpression decreases or increases the rate of endocytic site initiation, respectively. Ede1, an early endocytic Eps15-like protein important for endocytic initiation, is an Hrr25 target and is required for Hrr25 recruitment to endocytic sites. Hrr25 phosphorylation of Ede1 is required for Hrr25-Ede1 interaction and promotes efficient initiation of endocytic sites. These observations indicate that Hrr25 kinase and Ede1 cooperate to initiate and stabilize endocytic sites. Analysis of the mammalian homologs CK1δ/ε suggests a conserved role for these protein kinases in endocytic site initiation and stabilization.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Yutian
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Grassart
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Lu
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Catherine C L
authors.initials: CC
authors.lastname: Wong
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: John
authors.initials: J
authors.lastname: Yates
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
authors.forename: Georjana
authors.initials: G
authors.lastname: Barnes
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: drubin@berkeley.edu.
authors.forename: David G
authors.initials: DG
authors.lastname: Drubin
mesh_terms: D000818:Animals; D047389:Casein Kinase I / Q000378:metabolism*; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis / Q000502:physiology*; D006801:Humans; D010766:Phosphorylation / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D002966:Clathrin; C510360:Ede1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D047389:Casein Kinase I; C070775:HRR25 protein, S cerevisiae