title: Construction and application of a photo-cross-linked chemical array.
abstract: Chemical array technology is a powerful tool for high-throughput screening of small-molecule ligand-protein interactions. A chemical array is a collection of small-molecule compounds spotted and immobilized on a glass slide surface, providing a multiplex platform to identify small-molecule compounds binding to a protein of interest in high-throughput screening. Several research groups have developed a variety of methods for the immobilization of small molecules onto a solid matrix. We have developed a unique photo-cross-linked chemical array for immobilizing small molecules in a functional-group-independent manner. In this chapter, we describe in detail a protocol for the construction of a photo-cross-linked chemical array and its application for ligand screening by using a tag-fused protein.
authors.affiliation: Antibiotics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Kondoh
authors.forename: Kaori
authors.initials: K
authors.lastname: Honda
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Osada
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods*; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods; D054852:Small Molecule Libraries
chemical_list: D054852:Small Molecule Libraries