title: Impact of Donor Age on Corneal Endothelium-Descemet Membrane Layer Scroll Formation.
abstract: OBJECTIVE
  To correlate corneal endothelium-Descemet membrane (EDM) layer parameters of scroll tightness with donor age, endothelial cell density (ECD), and history of diabetes.
  
  
  METHODS
  Endothelium-Descemet membrane layer scrolls were harvested from 26 corneoscleral buttons using the SCUBA technique by a cornea-fellowship trained ophthalmologist masked to donor age. Two independent outcome parameters were used to characterize the scrolling severity of successfully harvested tissue: scroll width and tendency for EDM scroll formation (referred to as scroll rating on a 1-4 scale: incomplete scroll formation to tightly scrolled).
  
  
  RESULTS
  Mean donor age was 59 ± 17 (15-69) years. Mean ECD of EDM scroll was 2,451 ± 626 (range: 1,307-3,195) cells per square millimeter. Using stepwise linear regression, a significant correlation was found between scroll width and donor age (R=0.497, P<0.05). Additionally, a significant inverse correlation was found between scroll width and ECD (R=-0.605, P<0.05). There was no statistically significant correlation between a donor history of diabetes and the parameters of scrolling tendency.
  
  
  CONCLUSIONS
  Our data suggest that using older donors reduces EDM scroll tightness.
authors.affiliation: Department of Ophthalmology (A.B., W.M.P., V.V.M.), UT Southwestern, Dallas, TX; Department of Ophthalmology (S.M.), Alexandria Faculty of Medicine, Alexandria, Egypt; and Transplantation Services (D.D., H.D.C., J.P.M.), UT Southwestern, Dallas, TX.
authors.forename: Adam
authors.initials: A
authors.lastname: Bennett
authors.forename: Shahira
authors.initials: S
authors.lastname: Mahmoud
authors.forename: Donna
authors.initials: D
authors.lastname: Drury
authors.forename: H Dwight
authors.initials: HD
authors.lastname: Cavanagh
authors.forename: James P
authors.initials: JP
authors.lastname: McCulley
authors.forename: W Matthew
authors.initials: WM
authors.lastname: Petroll
authors.forename: V Vinod
authors.initials: VV
authors.lastname: Mootha
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001696:Biomechanical Phenomena; D002452:Cell Count; D055954:Corneal Endothelial Cell Loss / Q000473:pathology*; D003886:Descemet Membrane / Q000502:physiology* / Q000601:surgery; D057111:Descemet Stripping Endothelial Keratoplasty; D003920:Diabetes Mellitus; D004728:Endothelium, Corneal / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D014019:Tissue Donors; D055815:Young Adult