title: Protein kinase CK2 enables regulatory T cells to suppress excessive TH2 responses in vivo.
abstract: The quality of the adaptive immune response depends on the differentiation of distinct CD4(+) helper T cell subsets, and the magnitude of an immune response is controlled by CD4(+)Foxp3(+) regulatory T cells (Treg cells). However, how a tissue- and cell type-specific suppressor program of Treg cells is mechanistically orchestrated has remained largely unexplored. Through the use of Treg cell-specific gene targeting, we found that the suppression of allergic immune responses in the lungs mediated by T helper type 2 (TH2) cells was dependent on the activity of the protein kinase CK2. Genetic ablation of the β-subunit of CK2 specifically in Treg cells resulted in the proliferation of a hitherto-unexplored ILT3(+) Treg cell subpopulation that was unable to control the maturation of IRF4(+)PD-L2(+) dendritic cells required for the development of TH2 responses in vivo.
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Ulges
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Klein
authors.affiliation: Department of Pulmonary Medicine, III. Medical Clinic of the University Medical Center, Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Reuter
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Bastian
authors.initials: B
authors.lastname: Gerlitzki
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Hoffmann
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Grebe
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Valérie
authors.initials: V
authors.lastname: Staudt
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Natascha
authors.initials: N
authors.lastname: Stergiou
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Toszka
authors.initials: T
authors.lastname: Bohn
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Till-Julius
authors.initials: TJ
authors.lastname: Brühl
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Muth
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Hajime
authors.initials: H
authors.lastname: Yurugi
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Krishnaraj
authors.initials: K
authors.lastname: Rajalingam
authors.affiliation: Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Iris
authors.initials: I
authors.lastname: Bellinghausen
authors.affiliation: Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Tuettenberg
authors.affiliation: Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Hahn
authors.affiliation: Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Reißig
authors.affiliation: Department of Immunology and Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Irma
authors.initials: I
authors.lastname: Haben
authors.affiliation: Department of Neurology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Frauke
authors.initials: F
authors.lastname: Zipp
authors.affiliation: Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Ari
authors.initials: A
authors.lastname: Waisman
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Hans-Christian
authors.initials: HC
authors.lastname: Probst
authors.affiliation: Experimentelle Stammzelltransplantation der Medizinische Klinik und Poliklinik II, Zentrum für Experimentelle Molekulare Medizin, Julius-Maximilians-Universität Würzburg, Würzburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Beilhack
authors.affiliation: INSERM, U823, Université Joseph Fourier-Grenoble1, Institut Albert Boniot, Faculté de Médecine, Domaine de la Merci, La Tronche, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Buchou
authors.affiliation: INSERM U1036, Institute de Recherches en Technologies et Sciences pour le Vivant/Biologie du Cancer et de l'Infection, Commissariat à l'Énergie Atomique et aux Énerigies Alternatives Grenoble, Grenoble, France.
authors.forename: Odile
authors.initials: O
authors.lastname: Filhol-Cochet
authors.affiliation: KinaseDetect, Aarslev, Denmark.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Boldyreff
authors.affiliation: Department of Immunology and Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Minka
authors.initials: M
authors.lastname: Breloer
authors.affiliation: Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Jonuleit
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Schild
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Edgar
authors.initials: E
authors.lastname: Schmitt
authors.affiliation: Institute for Immunology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Bopp
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000201:enzymology / Q000276:immunology; D047390:Casein Kinase II / Q000276:immunology*; D002454:Cell Differentiation / Q000276:immunology; D048708:Cell Growth Processes / Q000276:immunology; D002460:Cell Line; D003713:Dendritic Cells / Q000201:enzymology / Q000276:immunology; D051858:Forkhead Transcription Factors / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D050835:Interferon Regulatory Factors / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011956:Receptors, Cell Surface / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000201:enzymology / Q000276:immunology*; D018418:Th2 Cells / Q000201:enzymology / Q000276:immunology*; D000099218:Interferon Regulatory Factor-4
chemical_list: D047390:Casein Kinase II; D051858:Forkhead Transcription Factors; D050835:Interferon Regulatory Factors; D011956:Receptors, Cell Surface; D000099218:Interferon Regulatory Factor-4