title: A study of ER stress in rat model of cerebral venous sinus thrombosis.
abstract: Cerebral venous sinus thrombosis (CVST) is a rare form of stroke. The role of endoplasmic reticulum (ER) stress markers is well documented in arterial stroke but has not been evaluated in venous stroke. The present study has been undertaken to investigate the role of ER stress in rodent model of CVST. For inducing CVST, a cranial window was made to expose superior sagittal sinus (SSS). A strip of filter paper soaked with 40% ferric chloride was applied on exposed cranial window while in sham operated control 0.9% saline was used. Clinical evaluations were done on day 1, 2, and 7 for neurological deficit. Rota rod test and brain infarction volume were also measured. Brain tissue was collected from infarcted portion for further analysis using real time polymerase chain reaction and western blot technique for ER stress markers. Augmented expression of ER stress markers and up regulation of apoptotic genes were found in infarcted tissue. These markers improved on day 7. It is concluded that ER markers are up regulated at an early stage of CVST and may be important in pathophysiology.
authors.affiliation: Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareily Road, Lucknow, U.P. 226014, India.
authors.forename: Hari Shanker
authors.initials: HS
authors.lastname: Tiwari
authors.affiliation: Department of Endocrinology, Central Drug Research Institute, Lucknow, U.P. 226014, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Tripathi
authors.affiliation: Department of Endocrinology, Central Drug Research Institute, Lucknow, U.P. 226014, India.
authors.forename: Durga Prasad
authors.initials: DP
authors.lastname: Mishra
authors.affiliation: Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareily Road, Lucknow, U.P. 226014, India.
authors.forename: Jayantee
authors.initials: J
authors.lastname: Kalita
authors.affiliation: Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Raebareily Road, Lucknow, U.P. 226014, India. Electronic address: drukmisra@rediffmail.com.
authors.forename: Usha Kant
authors.initials: UK
authors.lastname: Misra
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D020520:Brain Infarction / Q000473:pathology; D059865:Endoplasmic Reticulum Stress / Q000235:genetics; D015870:Gene Expression; D008297:Male; D009043:Motor Activity; D017207:Rats, Sprague-Dawley; D012851:Sinus Thrombosis, Intracranial / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D013997:Time Factors
chemical_list: D015415:Biomarkers
keywords: Cerebral venous sinus thrombosis; Endoplasmic reticulum associated degradation; Endoplasmic reticulum stress; Stroke; Unfolded protein response