title: [Expression and effect of microRNA-214 in advanced hypopharyngeal carcinoma].
abstract: OBJECTIVE
  To investigate the expression of microRNA-214(miR-214) in advanced hypopharyngeal carcinoma tissues and its effects on the invasion, migration and colone formation of FaDu cells.
  
  
  METHODS
  miR-214 expression in 30 cases of advanced hypopharyngeal carcinoma tissues and normal hypopharyngeal mucosa tissues was detected by real-time quantitative polymerase chain reaction (RT-qPCR). miR-214 was upregulated through transfecting the overexpression vector hsa-mir-214 into FaDu cells. The influences of miR-214 upregulation on the invasion, migration, clone formation and Twist expression were measured by Transwell invasion, Transwell migration, plate clone formation and Western blot assays, respectively.
  
  
  RESULTS
  The expression of miR-214 in advanced hypopharyngeal carcinoma tissues (0.311 ± 0.206) was significantly less than normal hypopharyngeal mucosa tissues (1.620 ± 1.394; t = 5.09, P < 0.05) . The expression of miR-214 was notably upregulated after tranfected with hsa-mir-214 compared with the negative control group (t = 6.347, P < 0.05). The migration and invasion ability of FaDu cells transfeced with hsa-mir-214 was decreased by comparison with negative control cells (t = 11.6, P < 0.01; t = 6.499, P < 0.05). There was no significant difference of the average clony number and the cloning efficiency between the experimental and negative control groups (t = 0.592, P > 0.05).
  
  
  RESULTS
  of Western blot assay showed that, Twist expression in the miR-214-overexpressed group was apparently decreased compared with that in the control group (t = 6.545, P < 0.05).
  
  
  CONCLUSIONS
  miR-214 is expressed at a low level in advanced hypopharyngeal carcinoma tissues, and can obviously inhibit the invasion and migration abilities of FaDu cells, possibly because of its inhibiting effect on Twist expression. Additionally, miR-214 plays no significant role in the proliferation of FaDu cells.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sa
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Yakui
authors.initials: Y
authors.lastname: Mu
authors.forename: Gaoying
authors.initials: G
authors.lastname: Sun
authors.affiliation: Email: xuwhns@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs