title: c-MYB regulates cell growth and DNA damage repair through modulating MiR-143.
abstract: Radiotherapy is the most successful nonsurgical treatment for nasopharyngeal carcinoma (NCP). Although NPCs initially respond well to a full course of radiation, recurrence and metastasis are frequent. In this study, we found that down-regulated c-MYB expression was associated with increased radiation resistance and DNA damage repair ability. Interestingly, c-MYB was over-expressed in cancer tissues but not in the adjacent tissues. Down-regulation of c-MYB expression inhibited cell proliferation, and led to cell cycle arrest at the M phase in NPC cells. Luciferase and chromatin immunoprecipitation assays demonstrated that c-MYB transactivated miR-143 through direct binding to its promoter. Based on these results, c-MYB might target miR-143 in order to regulate stem cell properties, cell growth, apoptosis, and DNA damage repair.
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Lung Cancer Research Institute and Cancer Center, Guangdong Provincial People's Hospital, Guangzhou 510080, China.
authors.forename: Sipei
authors.initials: S
authors.lastname: Wu
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 250117, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ji
authors.affiliation: Cancer Institute, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Kaitai
authors.initials: K
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China. Electronic address: hejx@vip.163.com.
authors.forename: Jianxing
authors.initials: J
authors.lastname: He
mesh_terms: D002277:Carcinoma; D002460:Cell Line; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000528:radiation effects; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs; D020598:Proto-Oncogene Proteins c-myb
keywords: Cancer stem cell; Nasopharyngeal carcinoma; Radio-resistivity; c-MYB; miR-143