title: Continuous lactose fermentation by Clostridium acetobutylicum--assessment of solventogenic kinetics.
abstract: This work reports the results of a series of tests on the specific butanol production rate by Clostridium acetobutylicum continuous cultures. The tests were carried out using lactose as carbon source to mimic cheese-whey. A continuous stirred tank reactor equipped with a microfiltration unit was used. The dilution rate (D) ranged between 0.02 and 0.15h(-1) and the ratio R of the permeate stream rate to the stream fed to the reactor ranged between 14% and 95%. For each set of D and R values, the continuous cultures were characterized in terms of concentration of cells, acids and solvents. Results were processed to assess the concentration of acidogenic cells, solventogenic cells, spores and the specific butanol production rate. The max butanol productivity was 0.5gL(-1)h(-1) at D=0.1h(-1) and R=95%. The butanol productivity referred to solventogenic cells was expressed as a function of concentration of lactose, acids and butanol.
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Procentese
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Raganati
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy; Bioprocess Engineering, AlgaePARC, Wageningen University, PO Box 16, 6700AA Wageningen, The Netherlands. Electronic address: giolivie@unina.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Olivieri
authors.affiliation: Istituto di Ricerche sulla Combustione, Consiglio Nazionale delle Ricerche, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Maria Elena
authors.initials: ME
authors.lastname: Russo
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Salatino
authors.affiliation: Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Marzocchella
mesh_terms: D018533:Biomass; D000440:Butanols / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D004867:Equipment Design; D005285:Fermentation; D005374:Filtration / Q000295:instrumentation; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D008962:Models, Theoretical; D012997:Solvents
chemical_list: D000440:Butanols; D012997:Solvents; D002244:Carbon; D007785:Lactose
keywords: Butanol; Clostridium acetobutylicum; Kinetic model; Microfiltration; Solventogenesis