title: Construction of two Listeria ivanovii attenuated strains expressing Mycobacterium tuberculosis antigens for TB vaccine purposes.
abstract: Bacillus Calmette-Guerin (BCG) has failed in complete control of tuberculosis (TB), thus, novel tuberculosis vaccines are urgently needed. We have constructed several TB vaccine candidates, which are characterized by the use of Listeria ivanovii (LI) strain as an antigen delivery vector. Two L. ivanovii attenuated recombinant strains L. ivanovii△actAplcB-Rv0129c and L. ivanovii△actAplcB-Rv3875 were successfully screened. Results from genome PCR and sequencing showed that the Mycobacterium tuberculosis antigen gene cassette coding for Ag85C or ESAT-6 protein respectively had been integrated into LI genome downstream of mpl gene. Western blot confirmed the secretion of Ag85C or ESAT-6 protein from the recombinant LI strains. These two recombinant strains showed similar growth curves as wide type strain in vitro. In vivo, they transiently propagated in mice spleen and liver, and induced specific CD8(+) IFN-γ secretion. Therefore, in this paper, two novel LI attenuated strains expressing specific TB antigens were successfully constructed. The promising growth characteristics in mice immune system and the capability of induction of IFN-γ secretion make them of potential interest for development of TB vaccines.
authors.affiliation: Department of Public Health Laboratory Sciences, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, West China School of Public Health, Sichuan University, 16#, Section 3, Ren Min Nan Road, Chengdu, Sichuan 610041, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Public Health Laboratory Sciences, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, West China School of Public Health, Sichuan University, 16#, Section 3, Ren Min Nan Road, Chengdu, Sichuan 610041, PR China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Public Health Laboratory Sciences, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, West China School of Public Health, Sichuan University, 16#, Section 3, Ren Min Nan Road, Chengdu, Sichuan 610041, PR China.
authors.forename: Zongkai
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA; Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors.forename: Asma
authors.initials: A
authors.lastname: Khanniche
authors.affiliation: Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA; Shanghai Institute of Immunology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Department of Public Health Laboratory Sciences, Food Safety Monitoring and Risk Assessment Key Laboratory of Sichuan Province, West China School of Public Health, Sichuan University, 16#, Section 3, Ren Min Nan Road, Chengdu, Sichuan 610041, PR China. Electronic address: wangchuan@scu.edu.cn.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D005260:Female; D007114:Immunization / Q000379:methods; D007371:Interferon-gamma / Q000378:metabolism; D008087:Listeria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D008099:Liver / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000276:immunology*; D032581:Tuberculosis Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014613:Vaccines, Attenuated / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D032581:Tuberculosis Vaccines; D014613:Vaccines, Attenuated; C072681:Mycobacterium tuberculosis antigens; D007371:Interferon-gamma
keywords: Antigen delivery vector; Listeria ivanovii; TB vaccine