title: The anti-osteoporosis and antioxidant activities of chemical constituents from Chrysanthemum indicum flowers.
abstract: Two new compounds, chrysinoneside A (1) and (-)-trans-chrysanthenol-6-O-β-D-glucopyranoside (2), along with 17 known compounds (3-19) were isolated from Chrysanthemum indicum flowers. The total phenolic and flavonoid contents of various fractions were determined. The EtOAC fraction had the highest total phenolic content (525.84 ± 23.51 mg GAE/g DR) and the total flavonoid content (63.49 ± 3.32 mg QE/g DR). The EtOAc and water fractions showed the greatest peroxyl radical-scavenging capacity and the ability to reduce Cu(I) ions, with ORAC and CUPRAC values ranging from 24.00 ± 0.44 to 28.06 ± 1.35 and 16.90 ± 0.51 to 49.77 ± 0.97 μM, respectively. Compounds 5-11, 18, and 19 displayed strong effects in both peroxyl radical-scavenging and reducing capacity assays at a concentration of 10 μM. The anti-osteoporosis activity of these compounds was also evaluated. Compounds 10, 13, and 19 exhibited the most potent tartrate-resistant acid phosphatase activity in receptor activator of nuclear factor-κB ligand-induced osteoclastic RAW 264.7 cells with values of 105.95 ± 1.18, 110.32 ± 3.95, and 112.58 ± 6.42%, respectively.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Bui Thi Thuy
authors.initials: BT
authors.lastname: Luyen
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
authors.forename: Hae Dong
authors.initials: HD
authors.lastname: Jang
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D031202:Chrysanthemum / Q000737:chemistry*; D005419:Flavonoids / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D010010:Osteoclasts / Q000187:drug effects* / Q000378:metabolism; D010024:Osteoporosis; D010084:Oxidation-Reduction; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D053245:RANK Ligand / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D007527:Isoenzymes; D010636:Phenols; D010936:Plant Extracts; D053245:RANK Ligand; C505689:Tnfsf11 protein, mouse; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: (−)-trans-Chrysanthenol-6-O-β-D-glucopyranoside; Chrysanthemum indicum; anti-osteoporosis activity; antioxidant activity; chrysinoneside A