title: Isotropic superresolution imaging for fluorescence emission difference microscopy.
abstract: Fluorescence emission diffraction microscopy (FED) has proven to be an effective sub-diffraction-limited imaging method. In this paper, we theoretically propose a method to further enhance the resolving capability of FED. Using a coated mirror and only one objective lens, this method achieves not only the same axial resolution as 4Pi microscopy but also a higher lateral resolution. The point spread function (PSF) of our method is isotropic. According to calculations, the full width at half-maximum (FWHM) of the isotropic FED's PSF is 0.17λ along all three spatial directions. Compared with confocal microscopy, the lateral resolution is improved 0.7-fold, and the axial resolution is improved 3.1-fold. Simulation tests also demonstrate this method's advantage over traditional microscopy techniques.
authors.forename: Shangting
authors.initials: S
authors.lastname: You
authors.forename: Cuifang
authors.initials: C
authors.lastname: Kuang
authors.forename: Zihao
authors.initials: Z
authors.lastname: Rong
authors.forename: Xu
authors.initials: X
authors.lastname: Liu
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Ding
mesh_terms: D016880:Anisotropy; D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D007909:Lenses; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity