title: VGLUT2 controls heat and punctuate hyperalgesia associated with nerve injury via TRPV1-Cre primary afferents.
abstract: Nerve injury induces a state of prolonged thermal and mechanical hypersensitivity in the innervated area, causing distress in affected individuals. Nerve injury-induced hypersensitivity is partially due to increased activity and thereby sustained release of neurotransmitters from the injured fibers. Glutamate, a prominent neurotransmitter in primary afferents, plays a major role in development of hypersensitivity. Glutamate is packed in vesicles by vesicular glutamate transporters (VGLUTs) to enable controlled release upon depolarization. While a role for peripheral VGLUTs in nerve injury-induced pain is established, their contribution in specific peripheral neuronal populations is unresolved. We investigated the role of VGLUT2, expressed by transient receptor potential vanilloid (TRPV1) fibers, in nerve injury-induced hypersensitivity. Our data shows that removal of Vglut2 from Trpv1-Cre neurons using transgenic mice abolished both heat and punctuate hyperalgesia associated with nerve injury. In contrast, the development of cold hypersensitivity after nerve injury was unaltered. Here, we show that, VGLUT2-mediated glutamatergic transmission from Trpv1-Cre neurons selectively mediates heat and mechanical hypersensitivity associated with nerve injury. Our data clarifies the role of the Trpv1-Cre population and the dependence of VGLUT2-mediated glutamatergic transmission in nerve injury-induced hyperalgesia.
authors.affiliation: Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Rogoz
authors.affiliation: Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Ludvig
authors.initials: L
authors.lastname: Stjärne
authors.affiliation: Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Klas
authors.initials: K
authors.lastname: Kullander
authors.affiliation: Department of Neuroscience, Uppsala University, Uppsala, Sweden.
authors.forename: Malin C
authors.initials: MC
authors.lastname: Lagerström
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006614:Hindlimb / Q000293:injuries*; D006358:Hot Temperature; D006930:Hyperalgesia / Q000209:etiology* / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009475:Neurons, Afferent / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries*; D050916:TRPV Cation Channels / Q000378:metabolism*; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics* / Q000378:metabolism*
chemical_list: C493477:Slc17a6 protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; D050599:Vesicular Glutamate Transport Protein 2; D018698:Glutamic Acid