title: Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations.
abstract: Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included. HNA-3 genotyping was performed using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The observed frequencies of the HNA-3a/3a, HNA-3a/3b, and HNA-3b/3b genotypes were 0.528, 0.380, and 0.092 in central Thais and 0.600, 0.350, and 0.050 in northern Thais, respectively. The frequencies were used to estimate HNA-3 incompatibility and risk of HNA-3a alloimmunization. The HNA-3 incompatibility in central Thais (33.28%) was higher than northern Thais (28.75%), corresponding to a significantly higher probability of HNA-3a alloimmunization (P<0.05) similar to Japanese and Chinese populations. This study showed the high risk of HNA-3 incompatibility and alloimmunization, especially in central Thai blood donors. A molecular-based identification of the HNA-3 genotype of female donors is suggested to reduce the risk of TRALI following plasma and whole blood allogeneic transfusion.
authors.affiliation: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand.
authors.forename: Oytip
authors.initials: O
authors.lastname: Nathalang
authors.affiliation: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand.
authors.forename: Kamphon
authors.initials: K
authors.lastname: Intharanut
authors.affiliation: Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathumtani, Thailand.
authors.forename: Kanokpol
authors.initials: K
authors.lastname: Siriphanthong
authors.affiliation: National Blood Centre, Thai Red Cross Society, Bangkok, Thailand.
authors.forename: Siriporn
authors.initials: S
authors.lastname: Nathalang
authors.affiliation: Blood Bank Section, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Nipapan
authors.initials: N
authors.lastname: Leetrakool
mesh_terms: D044466:Asian People / Q000208:ethnology / Q000235:genetics*; D001782:Blood Donors; D001787:Blood Group Incompatibility / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D060005:Genotyping Techniques / Q000379:methods; D006801:Humans; D007518:Isoantibodies / Q000276:immunology; D007519:Isoantigens / Q000235:genetics* / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000276:immunology; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D013785:Thailand / Q000453:epidemiology
chemical_list: C518134:HNA-3a antigen, human; D007518:Isoantibodies; D007519:Isoantigens; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C484524:SLC44A2 protein, human