title: Sensitive detection of biothiols and histidine based on the recovered fluorescence of the carbon quantum dots-Hg(II) system.
abstract: In this paper, we presented a novel, rapid and highly sensitive sensor for glutathione (GSH), cysteine (Cys) and histidine (His) based on the recovered fluorescence of the carbon quantum dots (CQDs)-Hg(II) system. The CQDs were synthesized by microwave-assisted approach in one pot according to our previous report. The fluorescence of CQDs could be quenched in the presence of Hg(II) due to the coordination occurring between Hg(II) and functional groups on the surface of CQDs. Subsequently, the fluorescence of the CQDs-Hg(II) system was recovered gradually with the addition of GSH, Cys or His due to their stronger affinity with Hg(II). A good linear relationship was obtained from 0.10 to 20μmol L(-1) for GSH, from 0.20 to 45μmol L(-1) for Cys and from 0.50 to 60μmol L(-1) for His, respectively. This method has been successfully applied to the trace detection of GSH, Cys or His in human serum samples with satisfactory results. The proposed method was simple in design and fast in operation, which demonstrated great potential in bio-sensing fields.
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Hou
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Fengshuang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yan
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
authors.affiliation: College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China. Electronic address: dinglan@jlu.edu.cn.
authors.forename: Lan
authors.initials: L
authors.lastname: Ding
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003545:Cysteine / Q000097:blood*; D005978:Glutathione / Q000097:blood*; D006639:Histidine / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D008628:Mercury / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D006639:Histidine; D008628:Mercury; D005978:Glutathione; D003545:Cysteine
keywords: Bio-sensor; Biothiols; Carbon quantum dots; Fluorescence detection; Histidine