title: Bioethanol production from the dry powder of Jerusalem artichoke tubers by recombinant Saccharomyces cerevisiae in simultaneous saccharification and fermentation.
abstract: It has been found that recombinant Saccharomyces cerevisiae 6525 can produce high concentration of ethanol in one-step fermentation from the extract of Jerusalem artichoke tubers or inulin. However, the utilization rate of raw materials was low and the fermentation process was costly and complicated. Therefore, in this study, after the optimum processing conditions for ethanol production in fed-batch fermentation were determined in flask, the recombinant S. cerevisiae 6525 was first used to produce ethanol from the dry powder of Jerusalem artichoke tubers in 5-L agitating fermentor. After 72 h of fermentation, around 84.3 g/L ethanol was produced in the fermentation liquids, and the conversion efficiency of inulin-type sugars to ethanol was 0.453, or 88.6 % of the theoretical value of 0.511. This study showed high feasibility of bioethanol industrial production from the Jerusalem artichoke tubers and provided a basis for it in the future.
authors.affiliation: College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
authors.forename: Yi-Zhou
authors.initials: YZ
authors.lastname: Wang
authors.forename: Shan-Mei
authors.initials: SM
authors.lastname: Zou
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: He
authors.forename: Chang-Hai
authors.initials: CH
authors.lastname: Wang
mesh_terms: D061249:Batch Cell Culture Techniques; D019149:Bioreactors; D000431:Ethanol / Q000378:metabolism* / Q000600:supply & distribution; D005240:Feasibility Studies; D005285:Fermentation; D006368:Helianthus / Q000737:chemistry / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000431:Ethanol; D007444:Inulin