title: Effects of (R)- and (S)-propranolol hydrochloride enantiomers on the resonance Rayleigh scattering spectra with erythrosine B as probe and their analytical applications.
abstract: Propranolol, a chiral drug with two configurations, i.e., (R)-propranolol hydrochloride (RPH) and (S)-propranolol hydrochloride (SPH), has racemes that can be used in clinical diagnosis due to their synergistic effects. SPH has a β-receptor blocking effect, and RPH has an antiarrhythmic effect. In pH 4.6 Britton-Robinson (BR) buffer solution, both RPH and SPH can react with erythrosine B to form 1:1 ion-association complexes. In the SPH-Ery B reaction system, a remarkable enhancement of the resonance Rayleigh scattering (RRS) signal located at 338 nm was observed. However, a similar phenomenon was not obvious and was unstable in the RPH-Ery B reaction system. Based on this result, a simple, novel and sensitive method for the determination of SPH was proposed based on the RRS technique. The linear range and limit of detection were 0.0680~4.0 µg mL(-1) and 20.6 ng mL(-1), respectively. Additionally, the spectroscopic approaches of frequency doubling scattering (FDS) and second-order scattering (SOS) were also proposed for SPH detection in this article. The interaction information regarding the mechanism of the reaction, suitable reaction conditions, influencing factors and the effects of mixed solutions were our investigation aims. The method had been applied to the determination of SPH in fresh serum and urine samples of healthy human subjects with satisfactory results.
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China; School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, P. R. China; School of Chemistry and Environment Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing 404100, P. R. China. Electronic address: sxyjd7600@sina.com.
authors.forename: Jidong
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
authors.forename: Ernv
authors.initials: E
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, P. R. China.
authors.forename: Shang
authors.initials: S
authors.lastname: Zhou
authors.affiliation: School of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing 408100, P. R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Yang
mesh_terms: D004923:Erythrosine / Q000737:chemistry*; D006801:Humans; D008027:Light; D011433:Propranolol / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D011433:Propranolol; D004923:Erythrosine
keywords: (R)-propranolol hydrochloride; (S)-propranolol hydrochloride; Erythrosine B; Resonance Rayleigh scattering