title: Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings.
abstract: Developing molecular diagnostics in resource-poor settings is challenging. As such, we purpose-built a novel bridging flocculation assay for qualitative evaluation of isothermally amplified DNA by naked eye. The flocculation assay was dependent on pH, DNA polymer amounts and lengths. The method was first applied to the rapid and sensitive detection of important plant pathogens and subsequently extended to other pathogens across the animal kingdom to demonstrate the wide applications of our approach.
authors.affiliation: Centre for Personalized Nanomedicine, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, QLD 4072, Australia. m.trau@uq.edu.au.
authors.forename: E J H
authors.initials: EJ
authors.lastname: Wee
authors.forename: H Y
authors.initials: HY
authors.lastname: Lau
authors.forename: J R
authors.initials: JR
authors.lastname: Botella
authors.forename: M
authors.initials: M
authors.lastname: Trau
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000382:microbiology; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000191:economics; D005427:Flocculation; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics* / Q000302:isolation & purification; D019169:Jurkat Cells; D021141:Nucleic Acid Amplification Techniques / Q000191:economics / Q000379:methods*; D044224:Pseudomonas syringae / Q000235:genetics* / Q000302:isolation & purification; D052617:Solid Phase Microextraction / Q000379:methods
chemical_list: D004247:DNA