title: Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases.
abstract: The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening 'typical' as well as 'atypical' Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of 'typical' and 'atypical' EAEC pathotype.
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India. Electronic address: deepak.rawool@yahoo.com.
authors.forename: Deepak B
authors.initials: DB
authors.lastname: Rawool
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Jess
authors.initials: J
authors.lastname: Vergis
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Deepthi
authors.initials: D
authors.lastname: Vijay
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Dhaka
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Mamta
authors.initials: M
authors.lastname: Negi
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Manesh
authors.initials: M
authors.lastname: Kumar
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Amruta
authors.initials: A
authors.lastname: Nair
authors.affiliation: Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur 440 006, India.
authors.forename: Krupali V
authors.initials: KV
authors.lastname: Poharkar
authors.affiliation: Department of Veterinary Pathology, Nagpur Veterinary College, MAFSU, Nagpur 440 006, India.
authors.forename: Nitin V
authors.initials: NV
authors.lastname: Kurkure
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, 243 122, India.
authors.forename: S V S
authors.initials: SV
authors.lastname: Malik
authors.affiliation: National Institute of Biotic Stress Management, Raipur, Chhattisgarh 492 012, India.
authors.forename: S B
authors.initials: SB
authors.lastname: Barbuddhe
mesh_terms: D000818:Animals; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D036781:Fimbriae Proteins / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D014774:Virulence / Q000235:genetics
chemical_list: D029968:Escherichia coli Proteins; C063055:fimbrillin; D036781:Fimbriae Proteins
keywords: Diarrhoea; EAEC; HEp-2; PCR; fimA