title: Synthesis and cell-based screening of one-bead-one-compound peptide libraries.
abstract: Combinatorial one-bead-one-compound (OBOC) peptide library screening has proven to be a powerful tool for identification of small molecules, peptides, or peptidomimetics against a variety of specific targets such as cell surface receptors, protein kinases, proteases, and phosphatases. With each bead displaying many copies of a single chemical entity, millions of compounds can be rapidly synthesized and screened with whole-cell binding on-bead functional assays. Here we describe the methodology for the synthesis, screening, and sequence deconvolution of an OBOC peptide library analyzed for affinity to a cancer cell line.
authors.affiliation: Department of Chemistry, The University of Western Ontario, London, ON, Canada.
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Bononi
authors.forename: Leonard G
authors.initials: LG
authors.lastname: Luyt
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D019151:Peptide Library
chemical_list: D019151:Peptide Library