title: Novel Nanomonitor ultra-sensitive detection of troponin T.
abstract: BACKGROUND
  Troponin is the preferred biomarker for diagnosing myocardial infarction. Point of care devices have not matched the sensitivity of laboratory-based methods for measuring troponin. The Nanomonitor is a novel point-of-care device that uses the change in electrical impedance that occurs when a biomarker binds to its antibody, which is then correlated to the concentration of the target biomarker.
  
  
  METHODS
  Performance characteristics of the Nanomonitor were evaluated and compared to a standard laboratory-based method.
  
  
  RESULTS
  The limit of detection of the Nanomonior for troponin T was 0.0088ng/l. Total imprecission was 2.38% and 0.85% at troponin T concentrations of 73ng/l and 1800ng/l. The functional sensitivity (10% coeffecient of variation) was 0.329ng/l. The linear regression had a slope of 0.996 (95% confidence interval, 0.991, 1.002), r=1.00, and an intercept of 15.88ng/l (95% confidence interval, -68.39ng/l, 100.15ng/l). The mean difference between the assays was -7.54ng/l, determined by Bland-Altman analysis.
  
  
  CONCLUSIONS
  The Nanomonitor preliminary results have favorable performance characteristics for detecting troponin T in patient blood, provide results in 15min, and are portable. More research is needed.
authors.affiliation: Portland Veterans Affairs Medical Center, and Oregon Health & Science University, Division of Hospital Medicine, Portland, OR, United States.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Barrett
authors.affiliation: University of Texas at Dallas, Department of Bioengineering, Richardson, TX, United States.
authors.forename: Nandhinee
authors.initials: N
authors.lastname: Radha Shanmugam
authors.affiliation: University of Texas at Dallas, Department of Bioengineering, Richardson, TX, United States.
authors.forename: Anjan Panneer
authors.initials: AP
authors.lastname: Selvam
authors.affiliation: Oregon Health & Science University, Department of Pathology, Portland, OR, United States.
authors.forename: Steven C
authors.initials: SC
authors.lastname: Kazmierczak
authors.affiliation: University of Texas at Dallas, Department of Bioengineering, Richardson, TX, United States. Electronic address: Shalini.Prasad@utdallas.edu.
authors.forename: Shalini
authors.initials: S
authors.lastname: Prasad
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D036103:Nanotechnology / Q000379:methods*; D020107:Troponin T / Q000097:blood*
chemical_list: D020107:Troponin T
keywords: Functional sensitivity; Nanotechnology; Point-of-care; Troponin T