title: Bioassay-guided identification of bioactive molecules from traditional Chinese medicines.
abstract: Traditional Chinese medicines (TCMs) serve as a major source of a variety of drug lead compounds. In the process of natural products development, bioassay-guided isolation is a rapid and validated method for isolation of compounds with bioactivities. This chapter describes bioassay-guided separation and purification of compounds from the crude extracts of TCMs. Two approaches including size-exclusion chromatography (SEC) and high performance liquid chromatography (HPLC) are described in detail.
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Han
authors.forename: Jingyu
authors.initials: J
authors.lastname: Zhang
authors.forename: Wenni
authors.initials: W
authors.lastname: He
authors.forename: Pei
authors.initials: P
authors.lastname: Huang
authors.forename: Ayokunmi
authors.initials: A
authors.lastname: Oyeleye
authors.forename: Xueting
authors.initials: X
authors.lastname: Liu
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D001688:Biological Products; D004365:Drugs, Chinese Herbal