title: Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction.
abstract: We have developed a new ligase chain reaction-based colorimetric assay for detection of DNA methylation with ultrahigh sensitivity and selectivity. Using the proposed assay, as low as 0.01 fM methylated DNA can be detected by visualization of color changes of gold nanoparticles with the naked eye.
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province, P. R. China. lzpbd@snnu.edu.cn.
authors.forename: Fengxia
authors.initials: F
authors.lastname: Su
authors.forename: Limei
authors.initials: L
authors.lastname: Wang
authors.forename: Yueying
authors.initials: Y
authors.lastname: Sun
authors.forename: Chenghui
authors.initials: C
authors.lastname: Liu
authors.forename: Xinrui
authors.initials: X
authors.lastname: Duan
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Li
mesh_terms: D003124:Colorimetry / Q000379:methods*; D018899:CpG Islands / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D019175:DNA Methylation; D016678:Genome; D006046:Gold / Q000737:chemistry*; D022521:Ligase Chain Reaction; D053768:Metal Nanoparticles / Q000737:chemistry*
chemical_list: D006046:Gold; D004247:DNA