title: Association of cholesteryl ester transfer protein genotypes with paraoxonase-1 activity, lipid profile and oxidative stress in type 2 diabetes mellitus: A study in San Luis, Argentina.
abstract: OBJECTIVE
  Diabetic dyslipidemia is common in type 2 diabetes. The TaqIB polymorphism in cholesteryl ester transfer protein (CETP; B1 and B2 alleles; rs708272) is associated with changes in enzyme activity and lipid concentrations. The aim of the present study was to assess associations of CETP genotypes with lipoprotein profile, oxidant/anti-oxidant status and the plasma activity of paraoxonase-1 (PON-1) in a population of diabetic patients living in San Luis, Argentina.
  
  
  METHODS
  For oxidative stress status parameters, thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) levels, and catalase and PON-1 activity were assessed in 40 patients with type 2 diabetes mellitus and 30 healthy participants. CETP polymorphism was analyzed by polymerase chain reaction-based methods.
  
  
  RESULTS
  Type 2 diabetes mellitus had significantly higher concentrations of oxidative stress parameters: TBARS (P < 0.0001) and catalase activity (P < 0.0001). PON-1 activity and NO levels were significantly lower in diabetics (P = 0.0002 and P = 0.0008, respectively). The CETP genotypes distribution among study groups was not significantly different. The B2 carriers of the TaqIB CETP polymorphism are associated with higher high-density lipoprotein cholesterol levels and PON-1 activity in control and type 2 diabetes mellitus patients. Linear regression analysis showed that there was a significant and positive correlation between the changes of PON-1 activity and high-density lipoprotein cholesterol levels in non-B1B1 (B2 carriers) in controls (r = 0.83, P < 0.0001) and diabetic patients (r = 0.39, P = 0.0003).
  
  
  CONCLUSIONS
  The results of the current study show that type 2 diabetes mellitus is characterized by intense oxidative stress, and that the alterations observed in the lipoprotein profile and PON-1 activity might be related to the higher CETP activity in diabetic patients as a consequence of insulin resistance.
authors.affiliation: National University of San Luis San Luis, Argentina.
authors.forename: Susana
authors.initials: S
authors.lastname: Siewert
authors.affiliation: National University of San Luis San Luis, Argentina.
authors.forename: Irma Ines
authors.initials: II
authors.lastname: Gonzalez
authors.affiliation: Juana Koslay Health Center San Luis, Argentina.
authors.forename: Roberto Osvaldo
authors.initials: RO
authors.lastname: Lucero
authors.affiliation: National University of San Luis San Luis, Argentina.
authors.forename: Marta Susana
authors.initials: MS
authors.lastname: Ojeda
keywords: Oxidative stress; Polymorphism; Type 2 diabetes mellitus