title: FcUni-RLuc: an engineered Renilla luciferase with Fc binding ability and light emission activity.
abstract: A novel and advanced Fc-binding probe – FcUni-RLuc namely – has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence – HWRGWV – was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.
authors.affiliation: Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran. Sci_rahman@yahoo.com r.emamzadeh@sci.ui.ac.ir.
authors.forename: A
authors.initials: A
authors.lastname: Farzannia
authors.forename: R
authors.initials: R
authors.lastname: Roghanian
authors.forename: S H
authors.initials: SH
authors.lastname: Zarkesh-Esfahani
authors.forename: M
authors.initials: M
authors.lastname: Nazari
authors.forename: R
authors.initials: R
authors.lastname: Emamzadeh
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000235:genetics / Q000378:metabolism*; D001681:Biological Assay / Q000379:methods; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000235:genetics / Q000378:metabolism*; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D015335:Molecular Probes; D011485:Protein Binding; D015202:Protein Engineering; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D000068878:Trastuzumab; D014407:Tumor Cells, Cultured
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D007141:Immunoglobulin Fc Fragments; D049410:Luciferases, Renilla; D015335:Molecular Probes; D018719:Erb-b2 Receptor Tyrosine Kinases; D000068878:Trastuzumab; C508053:ERBB2 protein, human