title: Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase.
abstract: OBJECTIVE
  The roots of Chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. The aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from Chlorophytum borivilianum (ISCB).
  
  
  METHODS
  The ISCB was screened using the carrageenan-induced paw edema, histamine-induced paw edema, cotton pellet-induced granuloma, and Freund's adjuvant-induced arthritis in rats at orally administered doses of 3, 10, and 30 mg/kg. Effect of ISCB on histone deacetylase (HDAC) level was measured by the HDAC assay at the highest dose (30 mg/kg).
  
  
  RESULTS
  The results showed that the ISCB significantly reduced carrageenan-induced inflammation, histamine-induced inflammation, cotton pellet-induced granuloma and Freund's adjuvant-induced arthritis in rats. The ISCB at a dose of 30 mg/kg significantly inhibited HDAC level in rat paw tissue.
  
  
  CONCLUSIONS
  It is concluded that saponins isolated from roots of C. borivilianum possess anti-inflammatory and anti-arthritic properties. ISCB may act by inhibiting histamine, prostaglandin and HDAC. This suggests that ISCBs have potential for therapeutic use in the treatment of inflammation and arthritis.
authors.affiliation: Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India.
authors.forename: Anirudha A
authors.initials: AA
authors.lastname: Lande
authors.affiliation: Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India; E-mail: shirishkumar77@yahoo.com.
authors.forename: Shirishkumar D
authors.initials: SD
authors.lastname: Ambavade
authors.affiliation: Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India.
authors.forename: Uma S
authors.initials: US
authors.lastname: Swami
authors.affiliation: Department of Pharmacology, JSPM's Jayawantrao Sawant College of Pharmacy and Research, University of Pune, Hadapasar, Pune-411028, India.
authors.forename: Prafulla P
authors.initials: PP
authors.lastname: Adkar
authors.affiliation: Haffkine Biopharmaceutical Corporation Ltd., Pimpri, Pune-411018, India.
authors.forename: Prashant D
authors.initials: PD
authors.lastname: Ambavade
authors.affiliation: Haffkine Biopharmaceutical Corporation Ltd., Pimpri, Pune-411018, India.
authors.forename: Arun B
authors.initials: AB
authors.lastname: Waghamare
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000188:drug therapy*; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000378:metabolism; D019668:Liliaceae / Q000737:chemistry*; D008297:Male; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use / Q000633:toxicity
chemical_list: D000893:Anti-Inflammatory Agents; D056572:Histone Deacetylase Inhibitors; D012503:Saponins; D006655:Histone Deacetylases