title: Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA.
abstract: A recent upgrade to the optics configuration of a thermal source FTIR microscope equipped with a focal plane array detector has enabled rapid acquisition of high magnification spectrochemical images, in transmission, with an effective geometric pixel size of ∼1 × 1 μm(2) at the sample plane. Examples, including standard imaging targets for scale and accuracy, as well as biomedical tissues and microorganisms, have been imaged with the new system and contrasted with data acquired at normal magnification and with a high magnification multi-beam synchrotron instrument. With this optics upgrade, one can now conduct rapid biodiagnostic ex vivo tissue imaging in-house, with images collected over larger areas, in less time (minutes) and with comparable quality and resolution to the best synchrotron source FTIR imaging capabilities.
authors.affiliation: Department of Chemistry, University of Manitoba, Winnipeg MB, Canada R3T2N2. Kathleen.Gough@umanitoba.ca.
authors.forename: C R
authors.initials: CR
authors.lastname: Findlay
authors.forename: R
authors.initials: R
authors.lastname: Wiens
authors.forename: M
authors.initials: M
authors.lastname: Rak
authors.forename: J
authors.initials: J
authors.lastname: Sedlmair
authors.forename: C J
authors.initials: CJ
authors.lastname: Hirschmugl
authors.forename: Jason
authors.initials: J
authors.lastname: Morrison
authors.forename: C J
authors.initials: CJ
authors.lastname: Mundy
authors.forename: M
authors.initials: M
authors.lastname: Kansiz
authors.forename: K M
authors.initials: KM
authors.lastname: Gough
mesh_terms: D000369:Aged, 80 and over; D016616:Cryoultramicrotomy; D017377:Diatoms / Q000166:cytology; D005260:Female; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors