title: [Differential expression of bone morphogenetic protein and activin membrane-bound inhibitor in mouse adipose tissues and primary preadipocytes].
abstract: OBJECTIVE
  To investigate the expression profiles of bone morphogenetic protein and activin membrane-bound inhibitor (BAMBI) during the development of mouse adipose tissue.
  
  
  METHODS
  The total RNA was extracted for real-time PCR for amplification of BAMBI mRNA from the suprascapular brown adipose tissue (BAT) and subcutaneous (inguinal) and visceral (gonadal) white adipose tissue (sWAT and vWAT, respectively) of mice at various embryonic and postnatal stages, as well as from isolated primary preadipocytes during differentiation.
  
  
  RESULTS
  In BAT, BAMBI mRNA levels exhibited a transient increase, peaking at day 0 (D0) and declined thereafter. sWAT and vWAT could be isolated from mice from postnatal D21 onwards, in which BAMBI mRNA levels were the highest and decreased at 8 weeks and 6 months. BAMBI mRNA levels were also significantly reduced in primary preadipocytes isolated from vWAT after induced differentiation. BAMBI mRNA expression level was higher in vWAT than in sWAT and BAT at the same developmental stages.
  
  
  CONCLUSIONS
  BAMBI is differentially expressed in different adipose tissues and developmental stages, which supports the hypothesis that BAMBI plays a pivotal role in the development of adipose tissues.
authors.affiliation: Department of Physiology and Pathophysiology; Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.E-mail: xluo@ mail.xjtu.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Luo
authors.forename: Ru
authors.initials: R
authors.lastname: Jia
authors.forename: Shuang-Yu
authors.initials: SY
authors.lastname: Wei
authors.forename: Ting
authors.initials: T
authors.lastname: Yao
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Wang
authors.forename: Chang
authors.initials: C
authors.lastname: Lu
authors.forename: Jonathan P Whitehead
authors.initials: JP
authors.lastname: Jonathan P Whitehead
authors.forename: Jian-Qun
authors.initials: JQ
authors.lastname: Yan
mesh_terms: D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D010766:Phosphorylation; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: C485672:Bambi protein, mouse; D019485:Bone Morphogenetic Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger