title: Square-edge intraocular lenses and epithelial lens cell proliferation: implications on posterior capsule opacification in an in vitro model.
abstract: BACKGROUND
  To evaluate lens epithelial cell (LEC) proliferation with two different designs (one-piece or three-piece) of hydrophobic acrylic IOLs with 360° square optic edge using an in vitro culture model of posterior capsule opacification (PCO).
  
  
  METHODS
  This experimental study was conducted at the Department of NEUROFARBA, Section of Pharmacology, University of Florence, Italy. Human LECs were seeded and cultured in transwell cell culture inserts coated with a type-IV collagen membrane on which an IOL (one-piece Tecnis-1 or three-piece AR40E, Abbott Medical Optics Inc.) had been previously placed. As control, cells were plated on the insert membrane without an IOL. At day six (cells confluent in controls) IOLs were removed and cell counting, viability and cell density under and outside the IOLs were evaluated.
  
  
  RESULTS
  No statistically significant difference in the number of cells (p>0.05) between inserts with the one-piece and three-piece IOLs was found. Cell density in the area under each IOL was significantly lower than in the area outside of it (p<0.05), or in the control insert. (p<0.05). Cell density under the single-piece IOL was not significantly different from that under the three-piece IOL (p>0.05).
  
  
  CONCLUSIONS
  A 360° sharp-edge played a crucial role in avoiding LEC migration under the IOL and preventing the formation of PCO after cataract surgery. Long term clinical evaluation is necessary to estimate functional results.
authors.affiliation: Department of Surgery and Translational Medicine - Eye Clinic, University of Florence, Florence, Italy. rita.mencucci@unifi.it.
authors.forename: Rita
authors.initials: R
authors.lastname: Mencucci
authors.affiliation: Department of Surgery and Translational Medicine - Eye Clinic, University of Florence, Florence, Italy. elefavuzza@virgilio.it.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Favuzza
authors.affiliation: Department of Surgery and Translational Medicine - Eye Clinic, University of Florence, Florence, Italy. carlottachok@live.it.
authors.forename: Carlotta
authors.initials: C
authors.lastname: Boccalini
authors.affiliation: Department of Ophthalmology, Poitiers University Hospital, Poitiers, Cedex, France. gicquelophtha@aol.com.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Gicquel
authors.affiliation: Department of NEUROFARBA, section of Pharmacology, University of Florence, Florence, Italy. laura.raimondi@unifi.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Raimondi
mesh_terms: D058442:Capsule Opacification / Q000517:prevention & control*; D002452:Cell Count; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D006801:Humans; D066298:In Vitro Techniques; D007910:Lenses, Intraocular; D008954:Models, Biological; D057852:Posterior Capsule of the Lens / Q000166:cytology*; D011183:Postoperative Complications / Q000517:prevention & control*; D011474:Prosthesis Design