title: Evaluation of the formation of a junctional DNA nanostructure through annealing curve analysis.
abstract: During the self-assembly of different numbers of oligonucleotides comprising junctional DNA nanostructures, a change in environmental variables (e.g., temperature or salt concentration) has a substantial influence on the final products. Further, distinctive annealing temperatures of oligonucleotides are observed depending on the state of hybridization. Here, we present an evaluation of the annealing characteristics of oligonucleotides for the formation of a simple junctional DNA nanostructure using an annealing curve analysis. This method may be useful for analyzing the formation of complex junctional DNA nanostructures.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea. Electronic address: seung3885@skku.edu.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea. Electronic address: kspcharlie@skku.edu.
authors.forename: Kyung Soo
authors.initials: KS
authors.lastname: Park
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, South Korea; SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 440-746, South Korea. Electronic address: sh.um@skku.edu.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D004247:DNA / Q000737:chemistry*; D008958:Models, Molecular; D049329:Nanostructures / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry; D012492:Salts / Q000737:chemistry; D013696:Temperature
chemical_list: D009841:Oligonucleotides; D012492:Salts; D004247:DNA
keywords: DNA; DNA nanostructure; Junctional DNA; qRT-PCR