title: Biomolecular recognition ability of RecA proteins for DNA on single-walled carbon nanotubes.
abstract: We examined the biomolecular recognition ability of RecA proteins using single-walled carbon nanotubes (SWNTs) wrapped with a single-stranded DNA (ssDNA) molecule as a mimic for the usual ssDNA molecules. The ssDNA-SWNT hybrids showed larger diameters compared to those of the usual ssDNA molecules. As a result, RecA molecules bound to the ssDNA-SWNTs, as observed using atomic force microscopy and agarose gel electrophoresis. On the other hand, when carboxymethylcellulose (CMC) was used rather than ssDNA, the RecA molecules did not bind to the CMC-SWNT hybrids. Our results indicate that RecA molecules recognize ssDNA on SWNT surfaces as DNA molecules through their biomolecular recognition ability.
authors.affiliation: Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan. Electronic address: 1214608@ed.tus.ac.jp.
authors.forename: Shusuke
authors.initials: S
authors.lastname: Oura
authors.affiliation: Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ito
authors.affiliation: Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nii
authors.affiliation: Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Homma
authors.affiliation: Department of Physics, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 1628601, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Umemura
mesh_terms: D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004277:DNA, Single-Stranded; D037742:Nanotubes, Carbon; D011938:Rec A Recombinases
keywords: Agarose gel electrophoresis; Atomic force microscopy; Carbon nanotube; Molecular recognition; RecA proteins; Single-stranded DNA