title: Plant cytokinesis: a tale of membrane traffic and fusion.
abstract: Cytokinesis separates the forming daughter cells. Higher plants have lost the ability to constrict the plasma membrane (PM) in the division plane. Instead, trans-Golgi network (TGN)-derived membrane vesicles are targeted to the centre of the division plane and generate, by homotypic fusion, the partitioning membrane named cell plate (CP). The CP expands in a centrifugal fashion until its margin fuses with the PM at the cortical division site. Mutant screens in Arabidopsis have identified a cytokinesis-specific syntaxin named KNOLLE and an interacting Sec1/Munc18 (SM) protein named KEULE both of which are required for vesicle fusion during cytokinesis. KNOLLE is only made during M-phase, targeted to the division plane and degraded in the vacuole at the end of cytokinesis. Here we address mechanisms of KNOLLE trafficking and interaction of KNOLLE with different soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) partners and with SM-protein KEULE, ensuring membrane fusion in cytokinesis.
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Jürgens
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Misoon
authors.initials: M
authors.lastname: Park
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Richter
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Touihri
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Krause
authors.affiliation: *ZMBP, Developmental Genetics, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Farid
authors.initials: F
authors.lastname: El Kasmi
authors.affiliation: †ZMBP, Microscopy, University of Tübingen, Auf der Morgenstelle 32, 72076 Tübingen, Germany.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Mayer
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000166:cytology*; D029681:Arabidopsis Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D048749:Cytokinesis; D006801:Humans; D008561:Membrane Fusion; D021381:Protein Transport
chemical_list: D029681:Arabidopsis Proteins