title: Marginal Leakage of Class V Composite Restorations Assessed Using Microcomputed Tomography and Scanning Electron Microscope.
abstract: OBJECTIVE
  The aim of the study was to compare in Class V composite restorations marginal leakage measurements obtained with microcomputed tomography (micro-CT) and scanning electron microscopy (SEM) observations.
  
  
  METHODS
  Class V cavities were prepared on 10 human molars and restored using Optibond FL (Kerr, Orange, CA, USA) and Premise Flowable (Kerr). Sealing ability was evaluated by assessing silver-nitrate penetration depth along enamel and dentin margins. Leakage was quantified using a scoring system. Micro-CT analysis provided 502 cross-sectional images for each tooth. Microleakage evaluation was performed first on three cross-sections corresponding to the sections examined by SEM, then on all 502 of the obtained micro-CT images. SEM observations were performed first at 20× magnification, then, if showing a zero score, at 80× magnification. Enamel and dentin microleakage scores assigned to corresponding sections through micro-CT and SEM (20×) were compared (Wilcoxon signed-rank test, α=0.05).
  
  
  RESULTS
  No statistically significant difference in leakage scores emerged between micro-CT and 20×-magnification SEM. Eight tooth sections that were given a zero score under SEM at 20× magnification showed to be infiltrated at the higher magnification (80×). For five teeth a higher score was assigned following scanning of 502 cross-sections than based on the observation of three sections.
  
  
  CONCLUSIONS
  Micro-CT presents as a valid, nondestructive in vitro method to quantitatively evaluate marginal leakage of adhesive restorations.
authors.forename: C
authors.initials: C
authors.lastname: Rengo
authors.forename: C
authors.initials: C
authors.lastname: Goracci
authors.forename: G
authors.initials: G
authors.lastname: Ametrano
authors.forename: N
authors.initials: N
authors.lastname: Chieffi
authors.forename: G
authors.initials: G
authors.lastname: Spagnuolo
authors.forename: S
authors.initials: S
authors.lastname: Rengo
authors.forename: M
authors.initials: M
authors.lastname: Ferrari
mesh_terms: D003188:Composite Resins; D003731:Dental Caries / Q000628:therapy; D003737:Dental Cavity Preparation; D003743:Dental Enamel; D003763:Dental Leakage / Q000000981:diagnostic imaging*; D018772:Dental Marginal Adaptation; D003793:Dental Restoration, Permanent; D003804:Dentin; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008963:Molar / Q000033:anatomy & histology / Q000000981:diagnostic imaging; D019279:Resin Cements; D055114:X-Ray Microtomography
chemical_list: D003188:Composite Resins; C469607:OptiBond FL; D019279:Resin Cements