title: The perivascular phenotype and behaviors of dedifferentiated cells derived from human mature adipocytes.
abstract: Derived from mature adipocytes, dedifferentiated fat (DFAT) cells represent a special group of multipotent cells. However, their phenotype and cellular nature remain unclear. Our study found that human DFAT cells adopted perivascular characteristics and behaviors. Flow cytometry and immunofluorescent staining revealed that human DFAT cells positively expressed markers highly related to perivascular cell lineages, such as CD140b, NG2 and desmin, but were negative for common endothelial markers, including CD31, CD34, and CD309. Furthermore, DFAT cells displayed vascular network formation ability in Matrigel, and they noticeably promoted and stabilized the vessel structures formed by human umbilical vascular endothelial cells (HUVECs) in vitro. These results provide novel evidence on the pericyte nature of human DFAT cells, further supporting the recent model for the perivascular origin of adult stem cells, in which tissue-specific progenitor cells in mesenchymal tissues associate with blood vessels, exhibiting perivascular characteristics and functions.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Song
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Kou
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Lu
authors.affiliation: School of Life Dentistry at Niigata, Nippon Dental University, Niigata 951-8580, Japan.
authors.forename: Atsunori
authors.initials: A
authors.lastname: Sugawara
authors.affiliation: School of Life Dentistry at Niigata, Nippon Dental University, Niigata 951-8580, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shimizu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Min-Ke
authors.initials: MK
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Li
authors.initials: L
authors.lastname: Du
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Life Dentistry at Niigata, Nippon Dental University, Niigata 951-8580, Japan.
authors.forename: Soh
authors.initials: S
authors.lastname: Sato
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Si Chuan 610041, China; School of Life Dentistry at Niigata, Nippon Dental University, Niigata 951-8580, Japan. Electronic address: shenjiefei@scu.edu.cn.
authors.forename: Jie-Fei
authors.initials: JF
authors.lastname: Shen
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D051929:CD146 Antigen / Q000378:metabolism; D054337:Cell Dedifferentiation; D002478:Cells, Cultured; D018920:Coculture Techniques; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D018919:Neovascularization, Physiologic; D010641:Phenotype; D011509:Proteoglycans / Q000378:metabolism; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000378:metabolism; D000099216:Chondroitin Sulfate Proteoglycan 4
chemical_list: D000941:Antigens; D015703:Antigens, CD; D051929:CD146 Antigen; D011509:Proteoglycans; D020797:Receptor, Platelet-Derived Growth Factor beta; D000099216:Chondroitin Sulfate Proteoglycan 4; C497902:MCAM protein, human
keywords: Angiogenesis; Dedifferentiated fat cell; Endothelial cell; Pericyte; Perivascular behavior