title: Increased pre-surgical numbers of endothelial progenitor cells and circulating endothelial cells in colorectal cancer fail to predict outcome.
abstract: BACKGROUND
  The endothelium and angiogenesis are therapeutic targets in cancer. Response to treatment may be assessed by laboratory plasma markers such as circulating endothelial cells (CECs), endothelial progenitor cells (EPCs), von Willebrand factor (vWf), soluble E selectin, vascular endothelial growth factor (VEGF) and angiogenin. We hypothesised that these markers, obtained before surgery, would predict 2-year outcome after surgery with or without anti-angiogenic therapy for colorectal cancer (CRC).
  
  
  METHODS
  We recruited 154 patients with CRC, of whom 51 were treated with surgery alone, 74 were treated with standard chemotherapy (5-fluorouracil) and 29 were treated with standard chemotherapy plus anti-VEGF therapy (Avastin). Peripheral blood was taken before surgery. CD34(+)/CD45(-)/CD146(+) CECs and CD34(+)/CD45(-)/CD309 [KDR](+) EPCs were measured by flow cytometry and plasma markers by ELISA.
  
  
  RESULTS
  After a mean of 2.1 years follow-up (range 1.9-2.3 years), 52 of the patients (33.7 %) experienced a poor outcome (radiological and/or histological evidence of tumour spread or recurrence, or death [n = 26]). In univariate analysis, poor outcome was linked to Dukes' stage (p < 0.001), American Joint Committee on Cancer (AJCC) stage (p < 0.001), type of treatment (surgery alone, standard chemotherapy with or without anti-antigenic therapy) (p = 0.047), CECs (p < 0.02) and EPCs (p < 0.01). In subsequent binary logistic regression analysis, only Dukes' stage (hazard ratio 2.3, 95 % confidence interval 1.0-5.3, p = 0.047) and modified AJCC stage (4.62, 1.88-11.33, p < 0.001) predicted a poor outcome.
  
  
  CONCLUSIONS
  Endothelial cell markers (CECs, EPCs, vWf, soluble E selectin) and growth factors (VEGF and angiogenin), measured before surgery, have nothing extra to offer in predicting 2-year outcome in colorectal cancer when compared to Dukes' or AJCC stage.
authors.affiliation: Centre for Cardiovascular Sciences City Hospital, University of Birmingham, Dudley Road, Birmingham, B18 7QH, UK.
authors.forename: K S
authors.initials: KS
authors.lastname: Ramcharan
authors.forename: G Y H
authors.initials: GY
authors.lastname: Lip
authors.forename: P S
authors.initials: PS
authors.lastname: Stonelake
authors.forename: A D
authors.initials: AD
authors.lastname: Blann
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood*; D002452:Cell Count; D003106:Colon / Q000098:blood supply / Q000473:pathology; D015179:Colorectal Neoplasms / Q000097:blood / Q000473:pathology* / Q000601:surgery*; D042783:Endothelial Cells / Q000378:metabolism*; D066026:Endothelial Progenitor Cells / Q000473:pathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D057234:Preoperative Period; D012007:Rectum / Q000098:blood supply / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor