title: Up-regulation of miR-9 target CBX7 to regulate invasion ability of bladder transitional cell carcinoma.
abstract: Bladder urothelial carcinoma is the most common genitourinary system cancer in China. The objective of this study was to investigate whether the miR-9 can regulate the invasion ability of human bladder transitional cell carcinoma cells by down-regulation of CBX7.
  
  
  
  The expression of miR-9 was detected by quantitative real-time PCR in bladder transitional cell carcinomas (TCC) and normal bladder transitional cell (NBTC) samples. Bioinformatics software was used to predict some potential target genes of miR-9. T24 cells were transfected with pre-miR-9, and the CBX7 protein expression was detected by Western blot. Luciferase activities assay was selected to verify that CBX7 was a direct and specific gene of miR-9. T24 cells were transfected with pcDNA-CBX7, and the expression of CBX7 gene was detected. Then, the transwell assay was used to detect the invasion ability of T24 cells with CBX7 over-expression.
  
  
  
  The expression of miR-9 increased significantly in human TCC specimens compared to that in NBTC specimens. TargetScan and PicTar software programs predicted CBX7 gene was a target gene of miR-9. The pre-miR-9 could up-regulate the miR-9 expression and down-regulate CBX7 protein expression. The luciferase activities assay verified that CBX7 gene was a direct and specific target gene of miR-9. The pcDNA-CBX7 transfection could up-regulate the CBX7 protein expression, and the invasion ability of T24 cells with CBX7 over-expression decreased significantly.
  
  
  
  Aberrantly expressed miR-9 contributes to T24 cells invasion, partly through directly down-regulating CBX7 protein expression in TCC. This miRNA signature offers a new potential therapeutic target for TCC.
authors.affiliation: Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Dalong
authors.initials: D
authors.lastname: Xie
authors.affiliation: Department of Neurobiology, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Chao
authors.initials: C
authors.lastname: Shang
authors.affiliation: Department of Urinary Surgery, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Central Laboratory, School of Stomatology, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, Liaoning, China (mainland).
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Tong
mesh_terms: D020413:3' Untranslated Regions; D002295:Carcinoma, Transitional Cell / Q000378:metabolism*; D002681:China; D019295:Computational Biology; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D001743:Urinary Bladder / Q000378:metabolism* / Q000473:pathology; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; C503627:CBX7 protein, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1