title: Energy transfer studies between Trp residues of three lipocalin proteins family, α1-acid glycoprotein, (orosomucoid), β-lactoglobulin and porcine odorant binding protein and the fluorescent probe, 1-aminoanthracene (1-AMA).
abstract: Energy transfer studies between Trp residues of α(1)-acid glycoprotein, β-lactoglobulin and porcine odorant binding protein (OBP) and the fluorescent probe 1-aminoanthracene (1-AMA) were performed. 1-AMA binds to the hydrophobic binding sites of the three proteins inducing a decrease in the fluorescence intensity of the Trp residues accompanied by an increase of that of 1-AMA. Our results indicate that 1-AMA is in close contact with hydrophobic tryptophan residue of β-lactoglobulin (Trp 19) to the difference of its binding to OBP, where Trp residues are far from the pocket and to α(1)-acid glycoprotein where three Trp residues are present at different areas of the protein.
authors.affiliation: Laboratoire de Biophysique Moléculaire, Université Lille Nord de France, Université de Lille 1, Bâtiment C6, 59655, Villeneuve d'Ascq, France, Jihad-Rene.Albani@Univ-lille1.fr.
authors.forename: Jihad R
authors.initials: JR
authors.lastname: Albani
authors.forename: Loïc
authors.initials: L
authors.lastname: Bretesche
authors.forename: Julie
authors.initials: J
authors.lastname: Vogelaer
authors.forename: Daniel
authors.initials: D
authors.lastname: Kmiecik
mesh_terms: D000818:Animals; D000873:Anthracenes / Q000737:chemistry*; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D007782:Lactoglobulins / Q000737:chemistry*; D009961:Orosomucoid / Q000737:chemistry*; D013552:Swine; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D000873:Anthracenes; D005456:Fluorescent Dyes; D007782:Lactoglobulins; D009961:Orosomucoid; D014364:Tryptophan; C049062:1-aminoanthracene