title: PON1 status evaluation in patients with chronic arterial occlusion of lower limbs due to atherosclerosis obliterans.
abstract: BACKGROUND
  Human paraoxonase (PON1) is a calcium-dependent enzyme physically associated with HDL, and it is believed to contribute to the atheroprotective effect of HDL. The aim of the study was to evaluate PON1 status in patients with atherosclerosis obliterans as an effect of ischemia regarding its activity and phenotype distribution.
  
  
  METHODS
  The study group consisted of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis obliterans (AO). The patients were divided into two groups according to the degree of ischemia: moderate (MI), and critical (CI). The ratio of the hydrolysis of salt-stimulated PON1 activity to the hydrolysis of phenylacetate was used to assign individuals to one of three possible phenotypes (low activity - A, medium activity - AB, high activity - B). It was observed that PON1 arylesterase activity was affected by ischemia of the lower limbs depending on its degree.
  
  
  RESULTS
  The odds ratio and the relative risk analysis showed that the patients with moderate ischemia are much more often characterized by phenotype A than by phenotype B. The low activity phenotype A occurs over twice as often in patients with chronic ischemia of the lower limbs as in individuals from the control group (OR = 2.125; 1.96 to 3.776, p = 0.0143).
  
  
  CONCLUSIONS
  This study presents the low activity phenotype A in relation to the risk of ischemia of the lower limbs due to atherosclerosis and shows the potentially important role of PON1 in conclusion of the process leading to intensification of ischemia degree.
authors.affiliation: Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Magdalena P
authors.initials: MP
authors.lastname: Kasprzak
authors.affiliation: Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Iskra
authors.affiliation: Department of General and Vascular Surgery, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Wacław
authors.initials: W
authors.lastname: Majewski
authors.affiliation: Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Budzyń-Napierała
authors.affiliation: Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Bogna
authors.initials: B
authors.lastname: Gryszczyńska
authors.affiliation: Department of General Chemistry, Chair of Chemistry and Clinical Biochemistry, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Strzyżewski
authors.affiliation: Department of Obstetrics and Gynecology with Pregnancy Pathology, Raszeja Hospital, Poznan, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kasprzak
keywords: arylesterase; low activity phenotype; paraoxonase activity; risk of ischemia