title: Interleukin-13 promotes expression of Alix to compromise renal tubular epithelial barrier function.
abstract: The epithelial barrier dysfunction plays a critical role in a number of kidney diseases. The mechanism is unclear. Alix is a protein involving in protein degradation in epithelial cells. This study aims to investigate that interleukin (IL)-13 inhibits Alix to compromise the kidney epithelial barrier function. In this study, the murine collecting duct cell line (M-1) was cultured in Transwell inserts to investigate the significance of Alix in compromising the epithelial barrier functions. T cell (Teff cells) proliferation assay was employed to assess the antigenicity of ovalbumin (OVA) that was transported across the M-1 monolayer barrier. The results showed that M-1 cells express Alix. Exposure to interleukin (IL)-13 markedly decreased the expression of Alix in M-1 cells, which compromised the M-1 monolayer barrier functions by showing the increases in the permeability to OVA. Over-expression of Alix abolished the IL-13-induced M-1 monolayer barrier dysfunction. Knockdown of Alix significantly increased M-1 monolayer permeability. The OVA collected from the Transwell basal chambers induced the OVA-specific T cell proliferation. We conclude that IL-13 compromises M-1 epithelial barrier functions via inhibiting Alix expression.
authors.affiliation: Department of Nephrology, Beijing Chao-Yang Hospital Affiliated to Capital Medical University, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.forename: Guangdong
authors.initials: G
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Qianmei
authors.initials: Q
authors.lastname: Sun
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Tong
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D017097:Electric Impedance; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D018793:Interleukin-13 / Q000494:pharmacology*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010047:Ovalbumin / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics; D019459:Urothelium / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D018793:Interleukin-13; C492144:Pdcd6ip protein, mouse; D010047:Ovalbumin
keywords: barrier function; biological transport; epithelial cells; kidney; renal tubules