title: Multimodal imaging of cutaneous wound tissue.
abstract: Quantitative assessment of wound tissue ischemia, perfusion, and inflammation provides critical information for appropriate detection, staging, and treatment of chronic wounds. However, few methods are available for simultaneous assessment of these tissue parameters in a noninvasive and quantitative fashion. We integrated hyperspectral, laser speckle, and thermographic imaging modalities in a single-experimental setup for multimodal assessment of tissue oxygenation, perfusion, and inflammation characteristics. Algorithms were developed for appropriate coregistration between wound images acquired by different imaging modalities at different times. The multimodal wound imaging system was validated in an occlusion experiment, where oxygenation and perfusion maps of a healthy subject's upper extremity were continuously monitored during a postocclusive reactive hyperemia procedure and compared with standard measurements. The system was also tested in a clinical trial where a wound of three millimeters in diameter was introduced on a healthy subject's lower extremity and the healing process was continuously monitored. Our in vivo experiments demonstrated the clinical feasibility of multimodal cutaneous wound imaging.
authors.affiliation: University of Science and Technology of China, School of Engineering Science, Hefei 230026, ChinabThe Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210, United States.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The Ohio State University, Department of Surgery, Columbus, Ohio 43210, United States.
authors.forename: Surya
authors.initials: S
authors.lastname: Gnyawali
authors.affiliation: The Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210, United States.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Huang
authors.affiliation: University of Science and Technology of China, School of Engineering Science, Hefei 230026, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Ren
authors.affiliation: The Ohio State University, Department of Surgery, Columbus, Ohio 43210, United States.
authors.forename: Gayle
authors.initials: G
authors.lastname: Gordillo
authors.affiliation: The Ohio State University, Department of Surgery, Columbus, Ohio 43210, United States.
authors.forename: Chandan K
authors.initials: CK
authors.lastname: Sen
authors.affiliation: University of Science and Technology of China, School of Engineering Science, Hefei 230026, ChinabThe Ohio State University, Department of Biomedical Engineering, Columbus, Ohio 43210, United States.
authors.forename: Ronald
authors.initials: R
authors.lastname: Xu
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D006801:Humans; D064847:Multimodal Imaging / Q000379:methods*; D010100:Oxygen / Q000032:analysis; D055420:Perfusion Imaging / Q000379:methods; D012867:Skin / Q000293:injuries* / Q000473:pathology*; D013817:Thermography / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D010100:Oxygen