title: Guided bone regeneration of mandibles using chitosan scaffold seeded with characterized uncultured omental adipose-derived stromal vascular fraction: an animal study.
abstract: OBJECTIVE
  The aim of this study was to assess the effect of characterized uncultured omental adipose-derived stromal vascular fraction (SVF) on bone regeneration in a circular mandibular bone defect. A chitosan scaffold was used to maintain the cells in situ.
  
  
  METHODS
  Seventy-five healthy male white Wistar rats were randomized into three experimental groups. In the control group, a surgical bone defect was created in the body of the mandible. In the scaffold group, a chitosan scaffold was placed into the defect. In the scaffold/SVF group, a scaffold was implanted and seeded with 10-μL SVF aliquots (2.19 ± 0.43 x 10(7) cells).
  
  
  RESULTS
  Microscopic and histomorphometric analyses showed improved and earlier regeneration of bone defects in the scaffold/SVF group compared to the scaffold-only group.
  
  
  CONCLUSIONS
  In this animal model, the use of a chitosan scaffold with SVF aliquots resulted in significantly earlier regeneration of bone than the use of the scaffold alone.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
authors.forename: Keyvan
authors.initials: K
authors.lastname: Amini
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000502:physiology*; D048271:Chitosan / Q000627:therapeutic use*; D003106:Colon / Q000166:cytology; D004195:Disease Models, Animal; D008297:Male; D008334:Mandible / Q000601:surgery*; D010012:Osteogenesis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017154:Stromal Cells; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan