title: Lineage specificity of primary cilia in the mouse embryo.
abstract: Primary cilia are required for vertebrate cells to respond to specific intercellular signals. Here we define when and where primary cilia appear in the mouse embryo using a transgenic line that expresses ARL13B-mCherry in cilia and Centrin 2-GFP in centrosomes. Primary cilia first appear on cells of the epiblast at E6.0 and are subsequently present on all derivatives of the epiblast. In contrast, extraembryonic cells of the visceral endoderm and trophectoderm lineages have centrosomes but no cilia. Stem cell lines derived from embryonic lineages recapitulate the in vivo pattern: epiblast stem cells are ciliated, whereas trophoblast stem cells and extraembryonic endoderm (XEN) stem cells lack cilia. Basal bodies in XEN cells are mature and can form cilia when the AURKA-HDAC6 cilium disassembly pathway is inhibited. The lineage-dependent distribution of cilia is stable throughout much of gestation, defining which cells in the placenta and yolk sac are able to respond to Hedgehog ligands.
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue New York, New York 10065, USA.
authors.forename: Fiona K
authors.initials: FK
authors.lastname: Bangs
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue New York, New York 10065, USA.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schrode
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue New York, New York 10065, USA.
authors.forename: Anna-Katerina
authors.initials: AK
authors.lastname: Hadjantonakis
authors.affiliation: Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue New York, New York 10065, USA.
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Anderson
mesh_terms: D020727:ADP-Ribosylation Factors / Q000378:metabolism; D000818:Animals; D064652:Basal Bodies / Q000378:metabolism; D002460:Cell Line; D019070:Cell Lineage; D002645:Chickens; D002923:Cilia / Q000378:metabolism*; D010064:Embryo Implantation; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism*; D004707:Endoderm / Q000166:cytology / Q000378:metabolism; D005260:Female; D054262:Gastrulation; D051379:Mice; D011247:Pregnancy; D015017:Yolk Sac / Q000166:cytology / Q000378:metabolism
chemical_list: C560093:Arl13b protein, mouse; D020727:ADP-Ribosylation Factors