title: Structural insight into the active site of a Bombyx mori unclassified glutathione transferase.
abstract: Glutathione transferases (GSTs) are major detoxification enzymes that play central roles in the defense against various environmental toxicants as well as oxidative stress. Here, we identify amino acid residues of an unclassified GST from Bombyx mori, bmGSTu-interacting glutathione (GSH). Site-directed mutagenesis of bmGSTu mutants indicated that amino acid residues Asp103, Ser162, and Ser166 contribute to catalytic activity.
authors.affiliation: a Faculty of Agriculture , Kyushu University Graduate School , Fukuoka , Japan.
authors.forename: Md Tofazzal
authors.initials: MT
authors.lastname: Hossain
authors.forename: Kohji
authors.initials: K
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D020134:Catalytic Domain; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation
chemical_list: D005982:Glutathione Transferase; D005978:Glutathione
keywords: Lepidoptera; glutathione; glutathione transferase; site-directed mutagenesis