title: Anti-inflammatory and antiatherogenic effects of the NLRP3 inflammasome inhibitor arglabin in ApoE2.Ki mice fed a high-fat diet.
abstract: BACKGROUND
  This study was designed to evaluate the effect of arglabin on the NLRP3 inflammasome inhibition and atherosclerotic lesion in ApoE2Ki mice fed a high-fat Western-type diet.
  
  
  RESULTS
  Arglabin was purified, and its chemical identity was confirmed by mass spectrometry. It inhibited, in a concentration-dependent manner, interleukin (IL)-1β and IL-18, but not IL-6 and IL-12, production in lipopolysaccharide and cholesterol crystal-activated cultured mouse peritoneal macrophages, with a maximum effect at ≈50 nmol/L and EC50 values for both cytokines of ≈ 10 nmol/L. Lipopolysaccharide and cholesterol crystals did not induce IL-1β and IL-18 production in Nlrp3(-/-) macrophages. In addition, arglabin activated autophagy as evidenced by the increase in LC3-II protein. Intraperitoneal injection of arglabin (2.5 ng/g body weight twice daily for 13 weeks) into female ApoE2.Ki mice fed a high-fat diet resulted in a decreased IL-1β plasma level compared with vehicle-treated mice (5.2±1.0 versus 11.7±1.1 pg/mL). Surprisingly, arglabin also reduced plasma levels of total cholesterol and triglycerides to 41% and 42%, respectively. Moreover, arglabin oriented the proinflammatory M1 macrophages into the anti-inflammatory M2 phenotype in spleen and arterial lesions. Finally, arglabin treatment markedly reduced the median lesion areas in the sinus and whole aorta to 54% (P=0.02) and 41% (P=0.02), respectively.
  
  
  CONCLUSIONS
  Arglabin reduces inflammation and plasma lipids, increases autophagy, and orients tissue macrophages into an anti-inflammatory phenotype in ApoE2.Ki mice fed a high-fat diet. Consequently, a marked reduction in atherosclerotic lesions was observed. Thus, arglabin may represent a promising new drug to treat inflammation and atherosclerosis.
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Amna
authors.initials: A
authors.lastname: Abderrazak
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Dominique
authors.initials: D
authors.lastname: Couchie
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Dler Faieeq Darweesh
authors.initials: DF
authors.lastname: Mahmood
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Rima
authors.initials: R
authors.lastname: Elhage
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Cécile
authors.initials: C
authors.lastname: Vindis
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Muriel
authors.initials: M
authors.lastname: Laffargue
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Véronique
authors.initials: V
authors.lastname: Matéo
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Berthold
authors.initials: B
authors.lastname: Büchele
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Monica Rubio
authors.initials: MR
authors.lastname: Ayala
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Menna
authors.initials: M
authors.lastname: El Gaafary
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Tatiana
authors.initials: T
authors.lastname: Syrovets
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Mohamed-Naceur
authors.initials: MN
authors.lastname: Slimane
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Bertrand
authors.initials: B
authors.lastname: Friguet
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Tamas
authors.initials: T
authors.lastname: Fulop
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Thomas
authors.initials: T
authors.lastname: Simmet
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.).
authors.forename: Khadija
authors.initials: K
authors.lastname: El Hadri
authors.affiliation: From Sorbonne Universités, UPMC Université Paris 06, Biological Adaptation and Ageing-IBPS, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); CNRS-UMR 8256, Paris, France (A.A., D.C., D.F.D.M., R.E.H., F.B., K.E.H., M.R.); Inserm U1164, Paris, France (A.A., D.C., D.F.D.M., R.E.H., B.F., K.E.H., M.R.); INSERM-UPS UMR 1048, Institut des Maladies Métaboliques et Cardiovasculaires, Toulouse, France (C.V., M.L.); Centre de Recherche d'Immunologie et Maladies Infectieuses, CIMI-Paris UPMC UMRS CR7, INSERM U-1135, CNRS ERL 8255, Hôpital Pitié-Salpêtrière, Paris, France (V.M.); Ulm University, Institute of Pharmacology of Natural Products and Clinical Pharmacology, Ulm, Germany (B.B., M.R.A., M.E.G., T.S., T.S.); Biochemistry Laboratory, Faculty of Medicine, TN-Monastir, Tunisia (M.-N.S.); and Centre de Recherche sur le Vieillissement, Service Gériatrique, Département de Médecine, Université de Sherbrooke, Sherbrooke, QC, Canada (T.F.). mustapha.rouis@snv.jussieu.fr.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Rouis
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use; D053329:Apolipoprotein E2 / Q000172:deficiency*; D050197:Atherosclerosis / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D059305:Diet, High-Fat / Q000009:adverse effects*; D005260:Female; D058847:Inflammasomes / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D012717:Sesquiterpenes / Q000494:pharmacology / Q000627:therapeutic use*; D045789:Sesquiterpenes, Guaiane; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D053329:Apolipoprotein E2; D002352:Carrier Proteins; D058847:Inflammasomes; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C492716:Nlrp3 protein, mouse; D012717:Sesquiterpenes; D045789:Sesquiterpenes, Guaiane; C083346:arglabin
keywords: arglabin-dimethylaminohydrochloride; atherosclerosis; cytokines; inflammasomes; inflammation