title: The recombinant maize ribosome-inactivating protein transiently reduces viral load in SHIV89.6 infected Chinese Rhesus Macaques.
abstract: Ribosome inactivating proteins (RIPs) inhibit protein synthesis by depurinating the large ribosomal RNA and some are found to possess anti-human immunodeficiency virus (HIV) activity. Maize ribosome inactivating protein (RIP) has an internal inactivation loop which is proteolytically removed for full catalytic activity. Here, we showed that the recombinant active maize RIP protected chimeric simian-human immunodeficiency virus (SHIV) 89.6-infected macaque peripheral blood mononuclear cells from lysis ex vivo and transiently reduced plasma viral load in SHIV89.6-infected rhesus macaque model. No evidence of immune dysregulation and other obvious side-effects was found in the treated macaques. Our work demonstrates the potential development of maize RIP as an anti-HIV agent without impeding systemic immune functions.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. wangrr1980@163.com.
authors.forename: Rui-Rui
authors.initials: RR
authors.lastname: Wang
authors.affiliation: Center for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, China. aaky73@yahoo.com.hk.
authors.forename: Ka-Yee
authors.initials: KY
authors.lastname: Au
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. hongyzheng@163.com.
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Zheng
authors.affiliation: Division of HIV/AIDS and STI Control, Yuxi Centers for Disease Control and Prevention, Yuxi 653100, Yunnan, China. glmcharge2012@gmail.com.
authors.forename: Liang-Min
authors.initials: LM
authors.lastname: Gao
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. snoopykm@126.com.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. luorh@mail.kiz.ac.cn.
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Luo
authors.affiliation: Center for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, China. sue.suelaw@gmail.com.
authors.forename: Sue Ka-Yee
authors.initials: SK
authors.lastname: Law
authors.affiliation: Center for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, China. amandamak@gmail.com.
authors.forename: Amanda Nga-Sze
authors.initials: AN
authors.lastname: Mak
authors.affiliation: Center for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, China. kbwong@cuhk.edu.hk.
authors.forename: Kam-Bo
authors.initials: KB
authors.lastname: Wong
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. mingxu2008-2009@163.com.
authors.forename: Ming-Xu
authors.initials: MX
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. pangw@mail.kiz.ac.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Pang
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. zhanggh@mail.kiz.ac.cn.
authors.forename: Gao-Hong
authors.initials: GH
authors.lastname: Zhang
authors.affiliation: Center for Protein Science and Crystallography, School of Life Sciences, the Chinese University of Hong Kong, Shatin N.T. 999077, Hong Kong, China. pcshaw@cuhk.edu.hk.
authors.forename: Pang-Chui
authors.initials: PC
authors.lastname: Shaw
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China. zhengyt@mail.kiz.ac.cn.
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000627:therapeutic use*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D008253:Macaca mulatta; D008297:Male; D011994:Recombinant Proteins / Q000627:therapeutic use; D054788:Ribosome Inactivating Proteins / Q000627:therapeutic use*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000097:blood / Q000188:drug therapy* / Q000821:virology; D015302:Simian Immunodeficiency Virus; D019562:Viral Load / Q000187:drug effects*; D003313:Zea mays
chemical_list: D044966:Anti-Retroviral Agents; D011994:Recombinant Proteins; D054788:Ribosome Inactivating Proteins