title: Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon.
abstract: Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Ruoyi
authors.initials: R
authors.lastname: Kang
authors.affiliation: Suzhou Academy of Agricultural Sciences, Suzhou 215155, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Qufu Normal University, Jining 273165, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address: yuzhifang@njau.edu.cn.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Yu
mesh_terms: D019659:Asteraceae / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism; D003521:Cyclopropanes / Q000737:chemistry / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D003521:Cyclopropanes; D009943:Organophosphorus Compounds; D010936:Plant Extracts; D010937:Plant Growth Regulators; D010940:Plant Proteins; C412563:1-methylcyclopropene; C005073:ethephon
keywords: 1-Methylcyclopropene; Ethephon; G. bicolor; Proteomics; Senescence