title: Fluorescent cellular assay for screening agents inhibiting Pseudomonas aeruginosa adherence.
abstract: Antibodies against Pseudomonas aeruginosa (PA) lectin, PAIIL, which is a virulence factor mediating the bacteria binding to epithelium cells, were prepared in chickens and purified from egg yolks. To examine these antibodies as a prophylactic agent preventing the adhesion of PA we developed a well plate assay based on fluorescently labeled bacteria and immortalized epithelium cell lines derived from normal and cystic fibrosis (CF) human lungs. The antibodies significantly inhibited bacteria adhesion (up to 50%) in both cell lines. In agreement with in vivo data, our plate assay showed higher susceptibility of CF cells towards the PA adhesion as compared to normal epithelium. This finding proved the reliability of the developed experimental system.
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. noskova.libuse@seznam.cz.
authors.forename: Libuše
authors.initials: L
authors.lastname: Nosková
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. bojkaku@gmail.com.
authors.forename: Božena
authors.initials: B
authors.lastname: Kubíčková
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. luci.vaskova@gmail.com.
authors.forename: Lucie
authors.initials: L
authors.lastname: Vašková
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. bara.blahova@volny.cz.
authors.forename: Barbora
authors.initials: B
authors.lastname: Bláhová
authors.affiliation: Central European Institute of Technology, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic. michaw@chemi.muni.cz.
authors.forename: Michaela
authors.initials: M
authors.lastname: Wimmerová
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. stiborov@natur.cuni.cz.
authors.forename: Marie
authors.initials: M
authors.lastname: Stiborová
authors.affiliation: Department of Biochemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 128 40 Prague 2, Czech Republic. hodek@natur.cuni.cz.
authors.forename: Petr
authors.initials: P
authors.lastname: Hodek
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001681:Biological Assay / Q000379:methods*; D015153:Blotting, Western; D002138:Calibration; D002645:Chickens; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D037102:Lectins / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D037102:Lectins