title: Modification of nanocellulose by poly-lysine can inhibit the effect of fumonisin B1 on mouse liver cells.
abstract: Fumonisin B1 is an important mycotoxin, mainly produced by Fusarium verticillioides. It has toxic effects on liver, brain, and kidney cells. The first aim of this study was to synthesize nanocellulose modified with poly-lysine (NMPL), and the second aim was to evaluate the adsorption of fumonisin B1 by NMPL. As third aim, the function of mouse liver cells was investigated after exposure to fumonisin B1, and fumonisin B1+ NMPL. In this study, NMPL was prepared using cross-linker, and then incubated with fumonisin B1 at controlled conditions. After incubation, the adsorption and release of fumonisin B1 were evaluated in each condition. Next, mouse liver cells were separately exposed to fumonisin B1, NMPL, and (fumonisin B1+NMPL). Then, the level of aniline aminotransferase (ALT) and aspartate aminotransferase (AST) was evaluated. It was found that both adsorption and release of fumonisin B1 were not affected by temperature and incubation time, but affected by pH and concentration of NMPL. Also, this study showed NMPL could adsorb fumonisin B1 in different foodstuffs. Importantly, although the levels of ALT and AST were increased when the cells were treated with fumonisin B1 alone, they were not affected when exposed to NMPL or (fumonisin B1+NMPL). The authors suggest that NMPL is a good adsorbent to remove and inhibit fumonisin B1.
authors.affiliation: Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jebali
authors.affiliation: Department of Food Science, College of Agriculture, Islamic Azad University, Yazd, Iran. Electronic address: Yasisnia.yasini@gmail.com.
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Yasini Ardakani
authors.affiliation: Department of Food Science, College of Agriculture, Islamic Azad University, Yazd, Iran. Electronic address: hshahdadi.2014@gmail.com.
authors.forename: Hossein
authors.initials: H
authors.lastname: Shahdadi
authors.affiliation: Department of Food Science, College of Agriculture, Islamic Azad University, Yazd, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Balal Zadeh
authors.affiliation: Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Seyedhossein
authors.initials: S
authors.lastname: Hekmatimoghaddam
mesh_terms: D000327:Adsorption; D000818:Animals; D002482:Cellulose / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D037341:Fumonisins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D037341:Fumonisins; D011107:Polylysine; C056933:fumonisin B1; D002482:Cellulose
keywords: Adsorption; Fumonisin B1; Liver; Nanocellulose; Poly-lysine