title: Characterization of the bovine gene LIPE and possible influence on fatty acid composition of meat.
abstract: LIPE is an intracellular neutral lipase, which is capable of hydrolyzing a variety of esters and plays a key role in the mobilization of fatty acids from diacylglycerols. The objectives of this study were to characterize the genetic polymorphism of bovine LIPE gene and to evaluate the possible association between three SNPs in the coding regions of this gene with the fatty acid composition of meat in a cattle population. Forty-three unrelated animals from different cattle breeds were re-sequenced and 21 SNPs were detected over approximately 2600 bp, five of these SNPs were novel. Three SNPs were selected, on the basis of evolutionary conservation, to perform validation and association studies in a crossbred cattle population. Our results may suggest a possible association of SNP1 with contents of oleic acid and total monounsaturated fatty acids (p < 0.01), and SNP2 and SNP3 with Heneicosylic acid content (p < 0.01), may be helpful to improve the quality of meat and improve health.
authors.affiliation: IGEVET, CCT LA PLATA CONICET, FCV, UNLP, La Plata B1900AVW, CC 296, Argentina ; Fellow of the Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Argentina.
authors.forename: Daniel Estanislao
authors.initials: DE
authors.lastname: Goszczynski
authors.affiliation: Unidad Integrada INTA Balcarce - FCA, UNMdP, Argentina.
authors.forename: Juliana Papaleo
authors.initials: JP
authors.lastname: Mazzucco
authors.affiliation: IGEVET, CCT LA PLATA CONICET, FCV, UNLP, La Plata B1900AVW, CC 296, Argentina.
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Ripoli
authors.affiliation: Unidad Integrada INTA Balcarce - FCA, UNMdP, Argentina.
authors.forename: Edgardo Leopoldo
authors.initials: EL
authors.lastname: Villarreal
authors.affiliation: IGEVET, CCT LA PLATA CONICET, FCV, UNLP, La Plata B1900AVW, CC 296, Argentina.
authors.forename: Andrés
authors.initials: A
authors.lastname: Rogberg-Muñoz
authors.affiliation: Unidad Integrada INTA Balcarce - FCA, UNMdP, Argentina.
authors.forename: Carlos Alberto
authors.initials: CA
authors.lastname: Mezzadra
authors.affiliation: Unidad Integrada INTA Balcarce - FCA, UNMdP, Argentina.
authors.forename: Lilia Magdalena
authors.initials: LM
authors.lastname: Melucci
authors.affiliation: IGEVET, CCT LA PLATA CONICET, FCV, UNLP, La Plata B1900AVW, CC 296, Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Giovambattista
keywords: ALBP, adipocyte lipid binding protein; Bovine; C18:1c9, oleic acid; C21:0, heneicosylic acid; Ct, C-terminal; F1, first filial; F2, second filial; GNRHR, gonadotropin-releasing hormone receptor; HWE, Hardy–Weinberg equilibrium; INTA, National Institute of Agricultural Technology; LIPE; LIPE, hormone-sensitive lipase; Lipid content; MUFA, total monounsaturated fatty acids; Nt, N-terminal; PCR, polymerase chain reaction; Polymorphism; R, regulatory module; SNP, single nucleotide polymorphism; he, unbiased expected heterozygosity; ho, observed heterozygosity; n, number of samples; p, p-value; pb, base pairs