title: Amperometric uric acid biosensor based on poly(vinylferrocene)-gelatin-carboxylated multiwalled carbon nanotube modified glassy carbon electrode.
abstract: In this study, a new uric acid biosensor was constructed based on ferrocene containing polymer poly(vinylferrocene) (PVF), carboxylated multiwalled carbon nanotubes (c-MWCNT) and gelatin (GEL) modified glassy carbon electrode (GCE). Uricase enzyme (UOx) was immobilized covalently through N-ethyl-N'-(3-dimethyaminopropyl) carbodiimide (EDC) and N-hydroxyl succinimide (NHS) chemistry onto c-MWCNT/GEL/PVF/GCE. The c-MWCNT/GEL/PVF composite was characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Various experimental parameters such as pH, applied potential, enzyme loading, PVF and c-MWCNT concentration were investigated in detail. Under the optimal conditions the dynamic linear range of uric acid was 2.0×10(-7) M-7.1×10(-4) M (R=0.9993) with the detection limit low to 2.3×10(-8) M. With good selectivity and sensitivity, the biosensor was successfully applied to determine the uric acid in human serum. The results of the biosensor were in good agreement with those obtained from standard method. Therefore, the presented biosensor could be a good promise for practical applications in real samples.
authors.affiliation: Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey. Electronic address: erdenpe@gmail.com.
authors.forename: Pınar Esra
authors.initials: PE
authors.lastname: Erden
authors.affiliation: Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
authors.forename: Ceren
authors.initials: C
authors.lastname: Kaçar
authors.affiliation: Department of Chemistry, Faculty of Science and Arts, Namık Kemal University, Tekirdağ, Turkey.
authors.forename: Funda
authors.initials: F
authors.lastname: Öztürk
authors.affiliation: Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
authors.forename: Esma
authors.initials: E
authors.lastname: Kılıç
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D037742:Nanotubes, Carbon / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D044967:Serum / Q000737:chemistry; D014503:Urate Oxidase / Q000737:chemistry; D014527:Uric Acid / Q000097:blood* / Q000737:chemistry
chemical_list: D005296:Ferrous Compounds; D037742:Nanotubes, Carbon; D011145:Polyvinyls; C451099:polyvinylferrocenium; D014527:Uric Acid; D002244:Carbon; D005780:Gelatin; D014503:Urate Oxidase
keywords: Amperometry; Biosensor; Carboxylated multiwalled carbon nanotube; Mediator; Poly(vinylferrocene); Uric acid