title: A new simulated plasma for assessing the solubility of mineral trioxide aggregate.
abstract: BACKGROUND
  Solubility of mineral trioxide aggregate (MTA) is an important characteristic that affects other properties such as microleakage and biocompatibility. Distilled water (DW) has previously been used for solubility tests. This experimental study compared the solubility of MTA in DW, synthetic tissue fluid (STF) and new simulated plasma (SP).
  
  
  METHODS
  In this study, 36 samples of tooth-colored ProRoot MTA were prepared and divided into three groups (n=12) to be immersed in three different solutions (DW, STF, and SP). Solubility tests were conducted at 2, 5, 9, 14, 21, 30, 50, and 78-day intervals. The unequal variance F-test (Welch test) was utilized to determine the effect of solubility media and Games-Howell analysis was used for pairwise comparisons. The repeated-measures ANOVA was used to assess the importance of immersion duration.
  
  
  RESULTS
  Welch test showed significant differences in solubility rates of samples between all the different solubility media at all the study intervals (P<0.05) except for the 14-day interval (P=0.094). The mixed repeated-measures ANOVA revealed a significant difference in solubility rate of MTA in three different solutions at all time-intervals (P=0.000). Games-Howell post-hoc test revealed that all pairwise comparisons were statistically significant at all time-intervals (P=0.000).
  
  
  CONCLUSIONS
  Based on the findings of this study, the long-term solubility of MTA in simulated plasma was less than that in synthetic tissue fluid and distilled water.
authors.affiliation: Department of Endodontics, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Mohammad
authors.initials: M
authors.lastname: Samiei
authors.affiliation: Department of Endodontics, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Shahriar
authors.initials: S
authors.lastname: Shahi
authors.affiliation: Department of Pediatric Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Naser
authors.initials: N
authors.lastname: Aslaminabadi
authors.affiliation: Department of Pharmaceutics, Tabriz University of Medical Sciences, Tabriz, Iran;
authors.forename: Hadi
authors.initials: H
authors.lastname: Valizadeh
authors.affiliation: Department of Oral Medicine, Tabriz University of Medical Sciences;
authors.forename: Zahra
authors.initials: Z
authors.lastname: Aghazadeh
authors.affiliation: Student Research Committee, Department of Prosthodontics, Tabriz University of Medical Sciences.
authors.forename: Seyyed Mahdi Vahid
authors.initials: SM
authors.lastname: Pakdel
keywords: Blood Substitute; MTA; Mineral Trioxide Aggregate; Plasma; Solubility