title: Evaluation of industrial Saccharomyces cerevisiae strains as the chassis cell for second-generation bioethanol production.
abstract: To develop a suitable Saccharomyces cerevisiae industrial strain as a chassis cell for ethanol production using lignocellulosic materials, 32 wild-type strains were evaluated for their glucose fermenting ability, their tolerance to the stresses they might encounter in lignocellulosic hydrolysate fermentation and their genetic background for pentose metabolism. The strain BSIF, isolated from tropical fruit in Thailand, was selected out of the distinctly different strains studied for its promising characteristics. The maximal specific growth rate of BSIF was as high as 0.65 h(-1) in yeast extract peptone dextrose medium, and the ethanol yield was 0.45 g g(-1) consumed glucose. Furthermore, compared with other strains, this strain exhibited superior tolerance to high temperature, hyperosmotic stress and oxidative stress; better growth performance in lignocellulosic hydrolysate; and better xylose utilization capacity when an initial xylose metabolic pathway was introduced. All of these results indicate that this strain is an excellent chassis strain for lignocellulosic ethanol production.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China.
authors.forename: Hongxing
authors.initials: H
authors.lastname: Li
authors.forename: Meiling
authors.initials: M
authors.lastname: Wu
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Jin
authors.initials: J
authors.lastname: Hou
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Bao
authors.forename: Yu
authors.initials: Y
authors.lastname: Shen
mesh_terms: D003470:Culture Media / Q000737:chemistry; D000431:Ethanol / Q000378:metabolism*; D005638:Fruit / Q000382:microbiology; D005947:Glucose / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009997:Osmotic Pressure; D018384:Oxidative Stress; D010429:Pentoses / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013785:Thailand
chemical_list: D003470:Culture Media; D010429:Pentoses; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D005947:Glucose