title: Rapid fluorescence spectroscopic characterization of salivary DNA of normal subjects and OSCC patients using ethidium bromide.
abstract: Recently, deoxyribonucleic acid (DNA) based biomarker(s) detection has been employed for cancer diagnosis. Earlier reports have suggested the presence of more DNA in the saliva of oral squamous cell carcinoma (OSCC) than normal by electrophoresis technique. Based on these, steady state and excited state kinetics of salivary DNA has been performed with 27 normal subjects and 67 OSCC patients saliva using ethidium bromide as a probe to look for the possibility in discrimination between them. On statistical analysis the sensitivity and specificity of 88.9 and 94.0 % has been achieved from the fluorescence emission spectra and 88.9 and 92.5 % with that of fluorescence excitation.
authors.affiliation: Department of Medical Physics, Anna University, Chennai, 600 025, India.
authors.forename: Manoharan
authors.initials: M
authors.lastname: Yuvaraj
authors.forename: Prakasarao
authors.initials: P
authors.lastname: Aruna
authors.forename: Dornadula
authors.initials: D
authors.lastname: Koteeswaran
authors.forename: Palraj
authors.initials: P
authors.lastname: Tamilkumar
authors.forename: Singaravelu
authors.initials: S
authors.lastname: Ganesan
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell; D004247:DNA / Q000032:analysis / Q000737:chemistry*; D004996:Ethidium / Q000737:chemistry*; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms; D012463:Saliva / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D004247:DNA; D004996:Ethidium