title: GJIC Enhances the phototoxicity of photofrin-mediated photodynamic treatment by the mechanisms related with ROS and Calcium pathways.
abstract: Despite initially positive responses, recurrences after Photodynamic treatment (PDT) can occur and there is need for improvement in the effectiveness of PDT. Our study uniquely showed that there was a significantly gap junctional intercellular communication (GJIC)-dependent PDT cytotoxicity. The presence of GJIC composed of Connexin 32 increased the PDT phototoxicity in transfected HeLa cells and in the xenograft tumors, and the enhanced phototoxicity of Photofrin-mediated PDT by GJIC was related with ROS and calcium pathways. Our study indicates the possibility that up-regulation or maintenance of gap junction functionality may be used to increase the efficacy of PDT. The phototoxicity effect of Photofrin was substantially greater in Dox-treated cells, which expressed the Cx32 and formed the GJ, than Dox-untreated.
authors.affiliation: Department of Pharmacology, Pharmacy School of Xuzhou Medical College, 221004, Xuzhou, P.R. China.|Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Pharmacy School of Xuzhou Medical College, 221004, Xuzhou, P.R. China.
authors.forename: Dengpan
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Gynaecology and Obstetrics, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, P.R. China.
authors.forename: Chengfang
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Cell & Systems Biology, University of Toronto, Ontario, M5S3G5, Canada.
authors.forename: Lucy
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China. wangqin6@mail.sysu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, 74 Zhongshan 2nd Road, Guangzhou, 510080, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Tao
mesh_terms: D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects* / Q000528:radiation effects; D002450:Cell Communication / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D017323:Dihematoporphyrin Ether / Q000378:metabolism / Q000494:pharmacology*; D017629:Gap Junctions / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000378:metabolism / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D017323:Dihematoporphyrin Ether; D002118:Calcium
keywords: calcium; gap junction intercellular communication; hela cell line; photodynamic treatment; photofrin; reactive oxygen species