title: Mesenchymal stem cells modified with nerve growth factor improve recovery of the inferior alveolar nerve after mandibular distraction osteogenesis in rabbits.
abstract: Distraction osteogenesis is widely used in the treatment of bony deformities and defects. However, injury to the inferior alveolar nerve is a concern. Our aim was to investigate the feasibility of using lentiviral-mediated human nerve growth factor beta (hNGFβ) of the inferior alveolar nerve in mandibular distraction osteogenesis in rabbits. To achieve this, mesenchymal stem cells (MSC) from the bone marrow of rabbit mandibles were isolated and genetically engineered using recombinant lentiviral vector containing hNGFβ. Twenty New Zealand white rabbits underwent mandibular distraction osteogenesis, and 5 million MSC transduced with hNGFβ-vector or control vector were transplanted around the nerve in the gap where the bone had been fractured during the operation (n=10 in each group). After gradual distraction, samples of the nerve were harvested for histological and histomorphometric analysis. We found that the genetically engineered MSC transduced by the lentiviral vector were able to secrete hNGFβ at physiologically relevant concentrations as measured by ELISA. Histological examination of the nerve showed more regenerating nerve fibres and less myelin debris in the group in which hNGFβ-modified MSC had been implanted than in the control group. Histomorphometric analysis of the nerve showed increased density of myelinated fibres in the group in which hNGFβ-modified MSC had been implanted than in the control group. The data suggest that implantation of hNGFβ-modified MSC can accelerate the morphological recovery of the inferior alveolar nerve during mandibular distraction osteogenesis in rabbits. The use of lentiviral-mediated gene treatment to deliver hNGFβ through MSC may be a promising way of minimising injury to the nerve.
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China. Electronic address: wanglzyh@fmmu.edu.cn.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Prosthodontics, Stomatology Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Oral and Maxillofacial Surgery, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Xi'an 710032, China. Electronic address: leidelin@fmmu.edu.cn.
authors.forename: D
authors.initials: D
authors.lastname: Lei
mesh_terms: D000818:Animals; D005240:Feasibility Studies; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D008297:Male; D008334:Mandible / Q000601:surgery*; D008340:Mandibular Nerve / Q000502:physiology* / Q000648:ultrastructure; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D009186:Myelin Sheath / Q000502:physiology / Q000648:ultrastructure; D009412:Nerve Fibers / Q000502:physiology / Q000648:ultrastructure; D009413:Nerve Fibers, Myelinated / Q000502:physiology / Q000648:ultrastructure; D020932:Nerve Growth Factor / Q000502:physiology*; D009416:Nerve Regeneration / Q000502:physiology; D019857:Osteogenesis, Distraction / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D011817:Rabbits; D011897:Random Allocation; D020127:Recovery of Function / Q000502:physiology; D014161:Transduction, Genetic / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D020932:Nerve Growth Factor
keywords: Bone marrow mesenchymal stem cells; Distraction osteogenesis; Gene therapy; Nerve growth factor; Nerve injury; Rabbit