title: Anti-hyperalgesic activity of crude extract and 7-methyljuglone of Diospyros lotus roots.
abstract: This study was designed to evaluate the antihyperalgesic effect of crude extract of Diospyros lotus followed by the isolation and characterisation of 7-methyljuglone in acetic acid and formalin tests. The pretreatment of crude extract evoked dose-dependent inhibition of noxious stimulation with maximum effect of 56.78% in acetic acid-induced writhing test, which were 51.89% and 60.69% in first and second phases, respectively, at 100 mg/kg i.p. The structure of 7-methyljuglone was confirmed by spectroscopic analysis. 7-Methyljuglone evoked profound increase in pain threshhold dose dependently; when it was studied in acetic acid-induced writhing test with 63.73% pain attenuation while 51.22% and 65.44% pain amelioration in first and second phases, respectively, at 100 mg/kg i.p. In conclusion, crude extract and 7-methyljuglone of D. lotus roots possessed both peripheral and central antinociceptive potential and thus could be a useful new therapeutic agent.
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors.forename: Abdur
authors.initials: A
authors.lastname: Rauf
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors.forename: Ghias
authors.initials: G
authors.lastname: Uddin
authors.affiliation: b Department of Pharmacy , Abdul Wali Khan University , Mardan 23200 , Pakistan.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: c H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi , Karachi 75270 , Pakistan.
authors.forename: Bina S
authors.initials: BS
authors.lastname: Siddiqui
authors.affiliation: a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , Pakistan.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arfan
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D029788:Diospyros / Q000737:chemistry*; D005260:Female; D008297:Male; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009285:Naphthoquinones / Q000302:isolation & purification / Q000494:pharmacology*; D010146:Pain / Q000188:drug therapy*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry
chemical_list: C517013:7-methyljuglone; D000700:Analgesics; D009285:Naphthoquinones; D010936:Plant Extracts
keywords: 7-methyljuglone; Diospyros lotus; antinociceptive activity; crude extract