title: Associative chemosensing by fluorescent macrocycle-dye complexes – a versatile enzyme assay platform beyond indicator displacement.
abstract: A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.
authors.affiliation: Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany. frankbiedermann@daad-alumni.de w.nau@jacobs-university.de.
authors.forename: Frank
authors.initials: F
authors.lastname: Biedermann
authors.forename: Denisa
authors.initials: D
authors.lastname: Hathazi
authors.forename: Werner M
authors.initials: WM
authors.lastname: Nau
mesh_terms: D001952:Bridged-Ring Compounds / Q000737:chemistry* / Q000378:metabolism; D004789:Enzyme Activation; D057075:Enzyme Assays; D004798:Enzymes / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007093:Imidazoles / Q000737:chemistry* / Q000378:metabolism; D047028:Macrocyclic Compounds / Q000737:chemistry* / Q000378:metabolism; D015394:Molecular Structure; D000099246:Cucurbit(n)urils
chemical_list: D001952:Bridged-Ring Compounds; D004798:Enzymes; D005456:Fluorescent Dyes; D007093:Imidazoles; D047028:Macrocyclic Compounds; C507198:cucurbit(8)uril; D000099246:Cucurbit(n)urils