title: In silico peptide prediction for antibody generation to recognize 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in genetically modified organisms.
abstract: For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.
authors.affiliation: CENPAT-CONICET, Centro Nacional Patagónico, Consejo Nacional de Investigaciones Científicas y Técnicas, Puerto Madryn, Argentina.
authors.forename: Mariela M
authors.initials: MM
authors.lastname: Marani
authors.forename: Joana
authors.initials: J
authors.lastname: Costa
authors.forename: Isabel
authors.initials: I
authors.lastname: Mafra
authors.forename: Maria Beatriz P P
authors.initials: MB
authors.lastname: Oliveira
authors.forename: Silvia A
authors.initials: SA
authors.lastname: Camperi
authors.forename: José Roberto de Souza Almeida
authors.initials: JR
authors.lastname: Leite
mesh_terms: D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase / Q000276:immunology*; D000906:Antibodies / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D010940:Plant Proteins / Q000276:immunology*; D030821:Plants, Genetically Modified / Q000276:immunology*; D013025:Glycine max / Q000276:immunology*
chemical_list: D000906:Antibodies; D000939:Epitopes; D010940:Plant Proteins; D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase
keywords: CP4-EPSPS; GMO; bioinformatics; in silico epitope prediction