title: MicroRNA 196B regulates FAS-mediated apoptosis in colorectal cancer cells.
abstract: Using miRNA microarray analysis, we identified 31 miRNAs that were significantly up-regulated or down-regulated in colon cancer tissues. We chose MIR196B, which was specifically up-regulated in colon cancer, for further study. We identified 18 putative MIR196B target genes by comparing between the mRNAs down-regulated in MIR196B-overexpressed cells and the assumed MIR196B target genes predicted by public bioinformatics tools. The association between MIR196B and FAS was verified in this study. FAS expression was constitutively elevated in normal human colorectal tissues. However, its expression was often reduced in human colorectal cancer. The decrease in FAS expression could be responsible for the reduction of apoptosis in colorectal cancer cells. In colorectal cancer tissue, we showed that MIR196B up-regulation was mutually followed by down regulation of FAS expression. We also showed that MIR196B directly repressed FAS expression in colorectal cells. Furthermore, anti-MIR196B up-regulated FAS expression and increased apoptosis in colorectal cancer cell lines. Our results suggest that the up-regulation of MIR196B modulates apoptosis in colorectal cancer cells by partially repressing FAS expression and that anti-MIR196B could be a potential candidate as an anti-cancer drug in colorectal cancer therapy.
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Mo
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Khondoker Jahengir
authors.initials: KJ
authors.lastname: Alam
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Kang
authors.affiliation: Department of Surgery, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Won Cheol
authors.initials: WC
authors.lastname: Park
authors.affiliation: Digestive Disease Research Institute, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Geom-Seog
authors.initials: GS
authors.lastname: Seo
authors.affiliation: Digestive Disease Research Institute, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Suck-Chei
authors.initials: SC
authors.lastname: Choi
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Hun-Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Hyung-Bae
authors.initials: HB
authors.lastname: Moon
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.|Digestive Disease Research Institute, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Ki-Jung
authors.initials: KJ
authors.lastname: Yun
authors.affiliation: Department of Pathology, School of Medicine, Wonkwang University, Iksan, Chonbuk, Republic of Korea.|Digestive Disease Research Institute, Wonkwang University, Iksan, Chonbuk, Republic of Korea.
authors.forename: Soo-Cheon
authors.initials: SC
authors.lastname: Chae
mesh_terms: D000368:Aged; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D016022:Case-Control Studies; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019295:Computational Biology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D019073:HT29 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction; D014162:Transfection; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
chemical_list: D000964:Antimetabolites, Antineoplastic; C491901:FAS protein, human; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; D019014:fas Receptor; C505407:CASP3 protein, human; C505520:CASP7 protein, human; D053148:Caspase 3; D053179:Caspase 7; D005472:Fluorouracil