title: Kinetic analysis of the weak affinity interaction between tris and lysozyme.
abstract: The biosensor based on total internal reflection imaging ellipsometry (TIRIE), regarded as an automotive real-time research approach for biomolecular interaction, is introduced to analyze the kinetic process of the weak interaction between tris and lysozyme. The experiment is performed by delivering lysozyme solution diluted to different concentrations to the biosensor substrate interface immobilized with tris. By applying pseudo-first-order interaction kinetics model, we are able to obtain the kinetic parameters from fitting experimental data. The calculated association rate constant and dissociation rate constant of tris and lysozyme interaction are in 10(-2) mol(-1) s(-1) and 10(3)s(-1) magnitude, respectively. To further improve TIRIE's ability for kinetically characterizing biomolecular interaction, a theoretical method to deduce associate rate constant before experiment is proposed.
authors.affiliation: NML, Institution of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China; University of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100049, China.
authors.forename: Tengfei
authors.initials: T
authors.lastname: Kang
authors.affiliation: NML, Institution of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China; University of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100049, China.
authors.forename: Wenxin
authors.initials: W
authors.lastname: Hao
authors.affiliation: NML, Institution of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Niu
authors.affiliation: NML, Institution of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China.
authors.forename: Ziren
authors.initials: Z
authors.lastname: Luo
authors.affiliation: NML, Institution of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China. Electronic address: gajin@imech.ac.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D007700:Kinetics; D009113:Muramidase / Q000378:metabolism*; D011485:Protein Binding; D014325:Tromethamine / Q000378:metabolism*
chemical_list: D014325:Tromethamine; D009113:Muramidase
keywords: Association rate constant; Kinetic analysis; Pseudo-first-order interaction kinetics; TIRIE biosensor; Weak affinity interaction