title: Sonic hedgehog elevates N-myc gene expression in neural stem cells.
abstract: Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Du
authors.affiliation: Chinese Academy of Medical Sciences, Department of Neurosurgery, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Medical Sciences, Department of Neurosurgery, Peking Union Medical College Hospital, Beijing 100730, China.
authors.forename: Renzhi
authors.initials: R
authors.lastname: Wang
keywords: N-myc gene; neural regeneration; neural stem cells; proliferation; sonic hedgehog signal pathway; stem cells; target gene