title: Investigating the CFH Gene Polymorphisms as a Risk Factor for Age-related Macular Degeneration in an Iranian Population.
abstract: Age-related macular degeneration (AMD) is a complex disorder which results in irreversible vision loss and progressive impairment of central vision. Disease susceptibility is influenced by multiple genetic and environmental factors. Single nucleotide polymorphisms (SNP) in the complement factor H gene are the most important genetic risk factors. We conducted a case-control study to investigate the association four SNPs (dbSNP ID: rs800292, rs1061170, rs2274700 and rs3753395) of CFH gene with AMD in the Iranian population.
  
  
  
  We recruited 100 AMD patients and 100 age- and sex-matched normal controls. Direct sequencing for three SNPs (rs800292, rs2274700 and rs3753395) and restriction fragment length polymorphism utilized for rs1061170. Allele and genotype frequencies of SNPs were calculated and tested for departure from Hardy-Weinberg equilibrium using the Chi-square test. An allelic and genotypic association was compared by logistic regression analysis using the SNPassoc.
  
  
  
  According to our results, the frequencies of risk allele for all SNPs (G, G, A, and C alleles of rs800292, rs2274700, rs3753395 and rs1061170, respectively) were significantly higher in AMD patients (p value < 0.001). AMD individuals who had at least one copy of the C allele of rs1061170 had an increased risk of disease compared with cases with the T allele. Other studied polymorphisms showed the same association.
  
  
  
  Our results suggest the contribution of all four predicted CFH polymorphisms in AMD susceptibility among the Iranian population. This association with CFH may lead to early detection and new strategies for prevention and treatment of AMD.
authors.affiliation: a Genetic Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran .
authors.forename: Mojgan
authors.initials: M
authors.lastname: Babanejad
authors.affiliation: b Ophthalmic Research Center and Ophthalmology Department , Shahid Beheshti University of Medical Sciences , Tehran , Iran .
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Moein
authors.affiliation: c Women's College Research Institute, Women's College Hospital, University of Toronto , Toronto , Canada , and.
authors.forename: Mohammad R
authors.initials: MR
authors.lastname: Akbari
authors.affiliation: a Genetic Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran .
authors.forename: Azadeh
authors.initials: A
authors.lastname: Badiei
authors.affiliation: d Department of Epidemiology and Biostatistics , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Yaseri
authors.affiliation: b Ophthalmic Research Center and Ophthalmology Department , Shahid Beheshti University of Medical Sciences , Tehran , Iran .
authors.forename: Masoud
authors.initials: M
authors.lastname: Soheilian
authors.affiliation: a Genetic Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran .
authors.forename: Hossein
authors.initials: H
authors.lastname: Najmabadi
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D017242:Complement Factor H / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D057092:Geographic Atrophy / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D057135:Wet Macular Degeneration / Q000175:diagnosis / Q000235:genetics*
chemical_list: C493495:CFH protein, human; D017242:Complement Factor H
keywords: Age-related macular degeneration; CFH gene; Iran; association study; polymorphisms; susceptibility