title: Isolation and characterization of six AP2/ERF transcription factor genes in Chrysanthemum nankingense.
abstract: The AP2/ERF family of plant transcription factors (TFs) regulate a variety of developmental and physiological processes. Here, we report the isolation of six AP2/ERF TF family genes from Chrysanthemum nankingense. On the basis of sequence similarity, one of these belonged to the Ethylene Responsive Factor (ERF) subfamily and the other five to the Dehydration Responsive Element Binding protein (DREB) subfamily. A transient expression experiment showed that all six AP2/ERF proteins localized to the nucleus. A yeast-one hybrid assay demonstrated that CnDREB1-1, 1-2 and 1-3 all function as transactivators, while CnERF1, CnDREB3-1 and 3-2 have no transcriptional activation ability. The transcription response of the six TFs in response to wounding, salinity and low temperature stress and treatment with abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) showed that CnERF1 was up-regulated by wounding and low temperature stress but suppressed by salinity stress. The transcription of CnDREB1-1, 1-2 and 1-3 was down-regulated by ABA and JA to varying degrees. CnDREB3-1 and 3-2 was moderately increased or decreased by wounding and SA treatment, suppressed by salinity stress and JA treatment, and enhanced by low temperature stress and ABA treatment.
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104110@njau.edu.cn.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Gao
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011204026@njau.edu.cn.
authors.forename: Peiling
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. aiping_song@aliyun.com.
authors.forename: Aiping
authors.initials: A
authors.lastname: Song
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. hb@njau.edu.cn.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2011104112@njau.edu.cn.
authors.forename: Yinjie
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2012204032@njau.edu.cn.
authors.forename: Liping
authors.initials: L
authors.lastname: Ren
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. 2012204028@njau.edu.cn.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Qi
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chenfd@njau.edu.cn.
authors.forename: Fadi
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. jiangjiafu@njau.edu.cn.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Jiang
authors.affiliation: College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. chensm@njau.edu.cn.
authors.forename: Sumei
authors.initials: S
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism; D031202:Chrysanthemum / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D019697:Onions / Q000166:cytology; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D019441:Plant Epidermis / Q000166:cytology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D010455:Peptides; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors