title: Solid-liquid separation method governs the in vitro bioaccessibility of metals in contaminated soil-like test materials.
abstract: An in vitro gastrointestinal model was used to explore the role of solid-liquid separation method on the bioaccessibility of trace elements in a smelter-impacted soil (NIST-2711) from Helena, MT and a mine overburden from an open-pit gold and silver mine in Mount Nansen, YK (YK-OVB). Separation methods studied included centrifugation (5,000 g, 12,000 g), syringe microfiltration (0.45 μm), and ultrafiltration (1,000 kDa, 50 kDa, 30 kDa, 10 kDa, 3 kDa). Results indicated that the use of syringe microfiltration generally yields the same bioaccessibility as the use of centrifugation and that the speed of centrifugation does not typically affect metal bioaccessibility. However, ultrafiltration consistently yields a significantly lower bioaccessibility than the use of centrifugation and syringe microfiltration. There are rarely any differences between bioaccessibility estimates generated using a low-resistance (1,000 kDa) and a high-resistance (3 kDa) ultrafiltration membrane; therefore, under the in vitro gastrointestinal conditions modeled herein, negligible quantities of trace elements are complexed to small molecules between 3 and 1,000 kDa. The primary exceptions to these trends were observed for Pb in NIST-2711 (5,000 g>12,000 g>0.45 μm>ultrafiltration) and for Tl in NIST-2711 and YK-OVB (5000 g∼12,000 g>0.45 μm>ultrafiltration). These results provide valuable information to researchers attempting to expand the use of in vitro bioaccessibility beyond soil Pb and As.
authors.affiliation: School of Public Health and Health Systems, University of Waterloo, Waterloo, Ontario, Canada. Electronic address: brian.laird@uwaterloo.ca.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Laird
authors.affiliation: Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Blake
authors.initials: B
authors.lastname: Weiseth
authors.affiliation: School of Public Health and Health Systems, University of Waterloo, Waterloo, Ontario, Canada.
authors.forename: Sara R
authors.initials: SR
authors.lastname: Packull-McCormick
authors.affiliation: Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Derek
authors.initials: D
authors.lastname: Peak
authors.affiliation: Royal Roads University, Victoria, British Columbia, Canada.
authors.forename: Matt
authors.initials: M
authors.lastname: Dodd
authors.affiliation: Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Siciliano
mesh_terms: D001682:Biological Availability; D004784:Environmental Monitoring / Q000379:methods*; D041981:Gastrointestinal Tract / Q000378:metabolism; D006801:Humans; D008670:Metals / Q000378:metabolism* / Q000493:pharmacokinetics; D008962:Models, Theoretical; D012989:Soil Pollutants / Q000378:metabolism* / Q000493:pharmacokinetics
chemical_list: D008670:Metals; D012989:Soil Pollutants
keywords: Bioavailability; Centrifugation; IVG; Microfiltration; PBET; Ultrafiltration