title: The accessible cellulose surface influences cellulase synergism during the hydrolysis of lignocellulosic substrates.
abstract: Effective enzymatic hydrolysis of insoluble cellulose requires the synergistic action of a suite of cellulase components. Most previous studies have only assessed cellulase synergism on model cellulosic substrates. When the actions of individual and combinations of cellulases (Cel7A, Cel6A, Cel7B, Cel5A) were assessed on various pretreated lignocellulosic substrates, Cel7A was shown to be the major contributor to overall cellulose hydrolysis, with the other enzymes synergistically enhancing its hydrolytic efficiency, at least partially, by facilitating Cel7A desorption (assessed by a double-sandwich enzyme-linked immunosorbent assay). When the influences of various substrate physicochemical characteristics on the effectiveness of enzyme synergism were assessed, a strong relationship was observed between cellulose accessibility (as determined by the cellulose binding module technique) and the degree of synergism, with greater synergy observed on the more disorganized/accessible cellulose surface.
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, Department of Wood Science, The University of British Columbia, 2424 Main Mall, Vancouver BC (Canada).
authors.forename: Jinguang
authors.initials: J
authors.lastname: Hu
authors.forename: Keith
authors.initials: K
authors.lastname: Gourlay
authors.forename: Valdeir
authors.initials: V
authors.lastname: Arantes
authors.forename: J S
authors.initials: JS
authors.lastname: Van Dyk
authors.forename: Amadeus
authors.initials: A
authors.lastname: Pribowo
authors.forename: Jack N
authors.initials: JN
authors.lastname: Saddler
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D044602:Cellulases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D013499:Surface Properties; D014242:Trichoderma / Q000201:enzymology
chemical_list: C036909:lignocellulose; D008031:Lignin; D044602:Cellulases
keywords: biomass; cellulose; enzymes; hydrolysis; reaction mechanisms