title: Development of gold nanoparticle based colorimetric method for quantitatively studying the inhibitors of Cu(2+)/Zn(2+) induced β-amyloid peptide assembly.
abstract: In this paper, a kind of gold nanoparticle (GNP)-based colorimetric assay has been developed for studying the reversible interaction of β-amyloid peptide (Aβ) with Cu(2+) and Zn(2+), and quantitatively analyzing four inhibitors (i.e., EDTA, EGTA, histidine and clioquinol) of Cu(2+)/Zn(2+) induced Aβ assembly. The inhibition efficiencies (e.g., half maximal inhibitory concentration, IC50 value) of these inhibitors could be measured in this work. As far as we know, these IC50 values were reported at the first time. In this assay, the streptavidin conjugated GNPs (SA-GNPs) were employed as indicators to monitor the Cu(2+)/Zn(2+) induced aggregating/disaggregating behaviors of biotin modified β-amyloid 1-16 peptides (Aβ1-16(biotin)). Because of high affinity of streptavidin (SA) with biotin, the aggregating/disaggregating of Aβ1-16(biotin) results in the significant color change of SA-GNPs. Furthermore, we demonstrate that the assay can be used as an effective tool for designing anti-dementia drugs through quantitative analysis of the interactions of four representative inhibitors with Cu(2+)/Zn(2+) induced Aβ assembly.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Wang
authors.affiliation: Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: wangkun@ujs.edu.cn.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: wangzx@ciac.ac.cn.
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000345:Affinity Labels; D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry; D001710:Biotin; D002712:Chlorides / Q000494:pharmacology*; D002942:Circular Dichroism; D007464:Clioquinol / Q000494:pharmacology; D003124:Colorimetry / Q000379:methods*; D019327:Copper Sulfate / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D006046:Gold / Q000737:chemistry*; D006639:Histidine / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D066329:Protein Aggregates / Q000187:drug effects*; D019809:Streptavidin; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D000345:Affinity Labels; D016229:Amyloid beta-Peptides; D002712:Chlorides; D010446:Peptide Fragments; D066329:Protein Aggregates; D017967:Zinc Compounds; C433241:amyloid beta-protein (1-16); D006639:Histidine; D004533:Egtazic Acid; D001710:Biotin; D006046:Gold; D007464:Clioquinol; C016837:zinc chloride; D019809:Streptavidin; D004492:Edetic Acid; D019327:Copper Sulfate
keywords: Alzheimer’s disease; Colorimetric assay; Cu(2+)/Zn(2+); Inhibitor; β-Amyloid