title: A rapid amplification/detection assay for analysis of Mycobacterium tuberculosis using an isothermal and silicon bio-photonic sensor complex.
abstract: Global tuberculosis (TB) control is hampered by cost and slow or insensitive diagnostic methods to be used for TB diagnosis in clinic. Thus, TB still remains a major global health problem. The failure to rapidly and accurately diagnose of TB has posed significant challenges with consequent secondary resistance and ongoing transmission. We developed a rapid Mycobacterium tuberculosis (MTB) amplification/detection method, called MTB isothermal solid-phase amplification/detection (MTB-ISAD), that couples isothermal solid-phase amplification and a silicon biophotonics-based detection sensor to allow the simultaneous amplification and detection of MTB in a label-free and real-time manner. We validated the clinical utility of the MTB-ISAD assay by detecting MTB nucleic acid in sputum samples from 42 patients. We showed the ability of the MTB-ISAD assay to detect MTB in 42 clinical specimens, confirming that the MTB-ISAD assay is fast (<20 min), highly sensitive, accurate (>90%, 38/42), and cost-effective because it is a label-free method and does not involve thermal cycling. The MTB-ISAD assay has improved time-efficiency, affordability, and sensitivity compared with many existing methods. Therefore, it is potentially adaptable for better diagnosis across various clinical applications.
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Agampodi Promoda
authors.initials: AP
authors.lastname: Perera
authors.affiliation: Department of Laboratory Medicine, Tan Tock Seng Hospital (TTSH), 11 Jalan Tan Tock Seng, Singapore 308433, Singapore.
authors.forename: Wen Ying
authors.initials: WY
authors.lastname: Tang
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Dong Liang
authors.initials: DL
authors.lastname: Fu
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Jack Kee
authors.initials: JK
authors.lastname: Sheng
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Gu
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Tae Yoon
authors.initials: TY
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Tan Tock Seng Hospital (TTSH), 11 Jalan Tan Tock Seng, Singapore 308433, Singapore.
authors.forename: Timothy
authors.initials: T
authors.lastname: Barkham
authors.affiliation: Institute of Microelectronics, Agency for Science, Technology and Research (A(⁎)STAR), 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
authors.forename: Mi
authors.initials: M
authors.lastname: Kyoung Park
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004867:Equipment Design; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012825:Silicon
keywords: DNA amplification/detection; Infectious disease; Mycobacterium tuberculosis; Silicon biophotonic sensor