title: Characterization of a new human plasma cell leukemia cell line UHKT-944.
abstract: OBJECTIVE
  A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia.
  
  
  RESULTS
  The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-kappa. VH3-9 region of IgVH genes was rearranged and somatically hypermutated. The UHKT-944 cells were found to be negative for most of tested B-cell, T-cell, and myeloid markers. According to cytogenetic analysis, the cells were classified as near tetraploid with several numerical and structural abnormalities including the t(14;20) involving IgH locus.
  
  
  CONCLUSIONS
  The established permanent plasma cell leukemia cell line is a suitable model for the study of cellular and molecular mechanisms of pathogenesis of this rare malignant disease.
authors.affiliation: Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic.|Department of Biochemistry, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Ilona
authors.initials: I
authors.lastname: Vyhlídalová
authors.affiliation: Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Uherková
authors.affiliation: Department of Immunology, Faculty of Science, Charles University, Prague, Czech Republic.
authors.forename: Markéta
authors.initials: M
authors.lastname: Pleschnerová
authors.affiliation: 1st Department of Internal Medicine, Division of Hematology, General Faculty Hospital, Charles University, Prague, Czech Republic.
authors.forename: Ivan
authors.initials: I
authors.lastname: Špička
authors.affiliation: Department of Cytogenetics, IHBT, Prague, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Březinová
authors.affiliation: Department of Cytogenetics, IHBT, Prague, Czech Republic.
authors.forename: Kyra
authors.initials: K
authors.lastname: Michalová
authors.affiliation: Leukemia PCR Diagnostics Laboratory, IHBT, Prague, Czech Republic.
authors.forename: Kamila
authors.initials: K
authors.lastname: Čermáková
authors.affiliation: Leukemia PCR Diagnostics Laboratory, IHBT, Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Polanská
authors.affiliation: Faculty of Science, Charles University, Prague, Czech Republic.|Faculty of Arts, Charles University, Prague, Czech Republic.
authors.forename: Petr L
authors.initials: PL
authors.lastname: Jedelský
authors.affiliation: Department of Experimental Virology, IHBT, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Hamšíková
authors.affiliation: Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic.
authors.forename: Kateřina
authors.initials: K
authors.lastname: Kuželová
authors.affiliation: Department of Cellular Biochemistry, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Stöckbauer
mesh_terms: D015415:Biomarkers; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D020732:Cytogenetic Analysis; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism; D016130:Immunophenotyping; D007952:Leukemia, Plasma Cell / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D007136:Immunoglobulins
keywords: human plasma cell line; interleukin-6 (IL-6); multiple myeloma; plasma cell; plasma cell leukemia