title: Gene expression microarray analysis of the spinal trigeminal nucleus in a rat model of migraine with aura.
abstract: Cortical spreading depression can trigger migraine with aura and activate the trigeminal vascular system. To examine gene expression profiles in the spinal trigeminal nucleus in rats following cortical spreading depression-induced migraine with aura, a rat model was established by injection of 1 M potassium chloride, which induced cortical spreading depression. DNA microarray analysis revealed that, compared with the control group, the cortical spreading depression group showed seven upregulated genes-myosin heavy chain 1/2, myosin light chain 1, myosin light chain (phosphorylatable, fast skeletal muscle), actin alpha 1, homeobox B8, carbonic anhydrase 3 and an unknown gene. Two genes were downregulated-RGD1563441 and an unknown gene. Real-time quantitative reverse transcription-PCR and bioinformatics analysis indicated that these genes are involved in motility, cell migration, CO2/nitric oxide homeostasis and signal transduction.
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ruozhuo
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Shengyuan
authors.initials: S
authors.lastname: Yu
authors.affiliation: Department of Neurology, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China.
authors.forename: Fengpeng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurology, the First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100037, China.
authors.forename: Enchao
authors.initials: E
authors.lastname: Qiu
keywords: cell migration; cortical spreading depression; enzyme; gene expression; migraine with aura; nervous system; neural regeneration; potassium chloride; regeneration; spinal nucleus of trigeminal nerve; ubiquitin degradation