title: Two-dimensional liquid chromatography with tandem mass spectrometry-based proteomic characterization of endometrial luminal epithelial surface proteins responsible for embryo implantation.
abstract: OBJECTIVE
  To identify endometrial epithelial cell surface proteins essential for blastocysts implantation.
  
  
  METHODS
  Isolation of cell-surface labeled prereceptive (pregnancy day 1) and receptive (pregnancy day 4) mouse endometrial proteins coupled to two-dimensional liquid chromatography with tandem mass spectrometry.
  
  
  METHODS
  University research laboratory.
  
  
  METHODS
  Sexually mature female imprinting control region (ICR) mice.
  
  
  METHODS
  Labeling, purification, and identification of endometrial luminal surface proteins with differentially expressing proteins determined by significant analysis of a microarray algorithm and selected differentially expressed proteins verified by immunohistochemistry and functional assay.
  
  
  METHODS
  Investigation in endometrial luminal surface proteome of prereceptive and receptive endometria of the expression of four of the differentially expressed proteins and functional analysis of aminopeptidase N in a three-dimensional blastocyst-endometrial coculture model.
  
  
  RESULTS
  We identified 104 cell surface proteins from prereceptive and receptive pregnant mouse endometria and found that 27 were statistically significantly up-regulated and 18 were statistically significantly down-regulated in the receptive endometrium. Immunohistochemical analysis of four of the differentially expressed proteins in the endometrium showed concordant results. Functional assay showed that blastocyst attachment was statistically significantly reduced upon inhibition of aminopeptidase N.
  
  
  CONCLUSIONS
  The luminal cell surface proteome of the prereceptive and receptive endometria differs, and aminopeptidase N is potentially involved in embryo attachment.
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong Shenzhen Hospital, The University of Hong Kong, Shenzhen, People's Republic of China.
authors.forename: Tian-Min
authors.initials: TM
authors.lastname: Ye
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong Shenzhen Hospital, The University of Hong Kong, Shenzhen, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China.
authors.forename: Ronald T K
authors.initials: RT
authors.lastname: Pang
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China.
authors.forename: Carmen O N
authors.initials: CO
authors.lastname: Leung
authors.affiliation: Open Laboratory for Tumor Biology, Department of Biochemistry, Shantou University College of Medicine, Shantou, People's Republic of China.
authors.forename: Jen-Fu
authors.initials: JF
authors.lastname: Chiu
authors.affiliation: Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China; Shenzhen Key Laboratory of Fertility Regulation, The University of Hong Kong Shenzhen Hospital, The University of Hong Kong, Shenzhen, People's Republic of China; Centre for Reproduction, Development and Growth, The University of Hong Kong, Pokfulam, Hong Kong, People's Republic of China. Electronic address: wsbyeung@hku.hk.
authors.forename: William S B
authors.initials: WS
authors.lastname: Yeung
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods; D010064:Embryo Implantation; D004717:Endometrium / Q000737:chemistry / Q000378:metabolism*; D004847:Epithelial Cells / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D008565:Membrane Proteins
keywords: Aminopeptidase N; embryo implantation; endometrial surface proteome; integrin-β1