title: Molecular characterization of a new wheat-Thinopyrum intermedium translocation line with resistance to powdery mildew and stripe rust.
abstract: A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. zhan030006@126.com.
authors.forename: Haixian
authors.initials: H
authors.lastname: Zhan
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. zxjemail@163.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. ligr28@uestc.edu.cn.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. pzh1990@aliyun.com.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. hujing56@126.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. leexinlee@aliyun.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. qiaoly1988@126.com.
authors.forename: Linyi
authors.initials: L
authors.lastname: Qiao
authors.affiliation: College of agronomy, Shanxi Agricultural University, Taigu 030801, China. jiajuqing@126.com.
authors.forename: Juqing
authors.initials: J
authors.lastname: Jia
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. zhanhaixian@aliyun.com.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Crop Science Institute, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China. wrczj@126.com.
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Chang
authors.affiliation: School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China. yangzujun@uestc.edu.cn.
authors.forename: Zujun
authors.initials: Z
authors.lastname: Yang
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D032461:Chromosomes, Plant / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005819:Genetic Markers; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D010935:Plant Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic / Q000235:genetics*; D014908:Triticum / Q000166:cytology / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*
chemical_list: D005819:Genetic Markers