title: A simplified and accurate detection of the genetically modified wheat MON71800 with one calibrator plasmid.
abstract: With the increasing number of genetically modified (GM) events, unauthorized GMO releases into the food market have increased dramatically, and many countries have developed detection tools for them. This study described the qualitative and quantitative detection methods of unauthorized the GM wheat MON71800 with a reference plasmid (pGEM-M71800). The wheat acetyl-CoA carboxylase (acc) gene was used as the endogenous gene. The plasmid pGEM-M71800, which contains both the acc gene and the event-specific target MON71800, was constructed as a positive control for the qualitative and quantitative analyses. The limit of detection in the qualitative PCR assay was approximately 10 copies. In the quantitative PCR assay, the standard deviation and relative standard deviation repeatability values ranged from 0.06 to 0.25 and from 0.23% to 1.12%, respectively. This study supplies a powerful and very simple but accurate detection strategy for unauthorized GM wheat MON71800 that utilizes a single calibrator plasmid.
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Jae-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Saet-Byul
authors.initials: SB
authors.lastname: Park
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Hyo-Jeong
authors.initials: HJ
authors.lastname: Roh
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Sunghoon
authors.initials: S
authors.lastname: Park
authors.affiliation: Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju 363-700, Republic of Korea.
authors.forename: Min-Ki
authors.initials: MK
authors.lastname: Shin
authors.affiliation: Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju 363-700, Republic of Korea.
authors.forename: Gui Im
authors.initials: GI
authors.lastname: Moon
authors.affiliation: Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Cheongju 363-700, Republic of Korea.
authors.forename: Jin-Hwan
authors.initials: JH
authors.lastname: Hong
authors.affiliation: Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea. Electronic address: hykim@khu.ac.kr.
authors.forename: Hae-Yeong
authors.initials: HY
authors.lastname: Kim
mesh_terms: D002138:Calibration; D018744:DNA, Plant / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant
keywords: Genetically modified wheat; MON71800; Qualification; Quantification; Standard reference plasmid