title: Sendai virus particle production: a more detailed role of F and HN through, namely, their association with M.
abstract: The Paramyxovirus membrane associated proteins are composed of two integral membrane glycoproteins, HN (H, G) and F, and of a matrix protein (M) carpeting the membrane inner layer. For Sendai virus (SeV), F and M have been proposed to form a complex at the endoplasmic reticulum that further migrates to the cell periphery where it represents a nucleation site for viral assembly completion (Essaidi-Laziosi et al., 2013). HN is recruited in the assembly complex once expressed at the cell surface. In contrast to F and M, HN appears dispensable for virus particle production. However, upon F suppression, concomitant HN suppression restricts viral particle production much more severely than F suppression alone, suggesting that HN plays a role as well. In this study, we demonstrate that the transmembrane and cytoplasmic regions of F are sufficient to promote virus particle production and incorporation of a foreign protein in viral particles. We further identify in the F cytoplasmic tail the site of interaction with M. We next confirm HN participation in viral particle production and we provide genetic evidence for a participation of M in the process. We finally derive observations that may provide a mechanism by which the viral C protein participates in viral particle production by mediating HN-M interaction.
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland. Electronic address: shevtsova.anastasia@gmail.com.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Shevtsova-Horoz
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland. Electronic address: essaidimanel@gmail.com.
authors.forename: Manel
authors.initials: M
authors.lastname: Essaidi-Laziosi
authors.affiliation: Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland. Electronic address: laurent.roux@unige.ch.
authors.forename: Laurent
authors.initials: L
authors.lastname: Roux
mesh_terms: D000818:Animals; D002460:Cell Line; D015707:HN Protein / Q000378:metabolism*; D011485:Protein Binding; D060066:Protein Interaction Maps; D029082:Sendai virus / Q000502:physiology*; D014760:Viral Fusion Proteins / Q000378:metabolism*; D014763:Viral Matrix Proteins / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D015707:HN Protein; C097020:M protein, Sendai virus; D014760:Viral Fusion Proteins; D014763:Viral Matrix Proteins
keywords: Deep sequencing; Matrix protein interactions; Mutant virus and phenotypic reversion; Sendai virus; Virus particle assembly