title: [Functional analysis of cancer-derived immunoglobulin G whole molecule-interacting proteins identified by LC-MS/MS].
abstract: OBJECTIVE
  To identify cancer-derived immunoglobulin G (IgG) whole molecule-interacting proteins to provide important clues for studying IgG biological functions.
  
  
  METHODS
  HeLa cell lysate was immunoprecipitated with rabbit antihuman IgG whole molecule antibody and normal rabbit IgG. The immunocomplex underwent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and was detected with silver staining. Three prominently enhanced bands were subjected to protein identification with liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the MS data were analyzed with Swiss-Prot database. Cancer-derived IgG whole molecule-interacting proteins were screened and functionally annotated.
  
  
  CONCLUSIONS
  We identified 6 potential cancer-derived IgG whole molecule-interacting proteins with co-immunoprecipitation combined with LC-MS/MS, which provides valuable clues for studying the function of cancer-derived IgG.
authors.affiliation: Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Department of Pathology, Shantou University Medical College, Shantou 515041, China.E-mail: wjp0128@aliyun.com.
authors.forename: Ju-Ping
authors.initials: JP
authors.lastname: Wang
authors.forename: Han-Ying
authors.initials: HY
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
mesh_terms: D000912:Antibodies, Neoplasm / Q000276:immunology*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D053719:Tandem Mass Spectrometry
chemical_list: D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D011506:Proteins