title: Experimental study on the effects of massive bowel resection on liver function and hepatocyte apoptosis.
abstract: OBJECTIVE
  The effects of short-bowel syndrome on liver function and liver morphology independent of parenteral nutrition have not been thoroughly investigated. Our aim was to investigate the effects of massive bowel resection on hepatocyte apoptosis and liver function in rats.
  
  
  METHODS
  A total of 37 female Sprague-Dawley rats were randomly assigned to five groups: Control (no procedure); Sham 1 [laparotomy (LT)/enterotomy (ET); evaluated on postoperative day (POD) 1]; Sham 2 (LT/ET; evaluated on POD7; Group 1 (80% bowel resection after LT/ET; POD1); and Group 2 (80% bowel resection; POD7). Blood samples were obtained for measuring aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase levels. For assessing hepatocyte apoptosis, liver tissue samples from the median lobe were obtained and used for a terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling assay.
  
  
  RESULTS
  Aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase levels showed statistically significant differences among the five groups. Apoptotic hepatocyte counts there were statistically significant differences among groups for counts made in 20 consecutive high-power fields. However, liver sinusoidal cell apoptosis rates among groups showed statistically significant differences for counts made in 20 consecutive high-power fields, particularly on POD7 in rats undergoing massive bowel resection.
  
  
  CONCLUSIONS
  Parenteral nutrition is not the only factor involved in liver dysfunction after massive bowel resection. Massive bowel resection alone can cause liver abnormalities. Rats undergoing massive small intestinal resection show significant temporal increases in liver sinusoidal cell apoptosis rates.
authors.affiliation: Department of General Surgery, Ankara Numune Teaching Hospital, Ankara, Turkey. baris104@yahoo.com.
authors.forename: Akın
authors.initials: A
authors.lastname: Bostanoğlu
authors.forename: Taner
authors.initials: T
authors.lastname: Oruğ
authors.forename: Barış Doğu
authors.initials: BD
authors.lastname: Yıldız
authors.forename: Sevil
authors.initials: S
authors.lastname: Işık
authors.forename: Neslihan İnci
authors.initials: Nİ
authors.lastname: Zengin
authors.forename: Ebru
authors.initials: E
authors.lastname: Evren
authors.forename: Gül Sevim
authors.initials: GS
authors.lastname: Saydam
mesh_terms: D000818:Animals; D017209:Apoptosis; D013505:Digestive System Surgical Procedures / Q000009:adverse effects; D005260:Female; D022781:Hepatocytes / Q000473:pathology*; D008099:Liver / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012778:Short Bowel Syndrome / Q000473:pathology* / Q000503:physiopathology*