title: Development and validation of sensitive kinetic spectrophotometric method for the determination of moxifloxacin antibiotic in pure and commercial tablets.
abstract: New, accurate, sensitive and reliable kinetic spectrophotometric method for the assay of moxifloxacin hydrochloride (MOXF) in pure form and pharmaceutical formulations has been developed. The method involves the oxidative coupling reaction of MOXF with 3-methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH) in the presence of Ce(IV) in an acidic medium to form colored product with lambda max at 623 and 660 nm. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 623 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity range was found to be 1.89-40.0 μg mL(-1) for initial rate and fixed time methods. The limit of detection for initial rate and fixed time methods is 0.644 and 0.043 μg mL(-1), respectively. Molar absorptivity for the method was found to be 0.89×10(4) L mol(-1) cm(-1). Statistical treatment of the experimental results indicates that the methods are precise and accurate. The proposed method has been applied successfully for the estimation of moxifloxacin hydrochloride in tablet dosage form with no interference from the excipients. The results are compared with the official method.
authors.affiliation: Department of Chemistry, Faculty of Sciences, University of Aleppo, Aleppo, Syria. Electronic address: profashour2010@myway.com.
authors.forename: Safwan
authors.initials: S
authors.lastname: Ashour
authors.affiliation: Department of Pharmaceutical Chemistry, Pharmacy Program, Batterjee Medical College, Jeddah, Saudi Arabia.
authors.forename: Roula
authors.initials: R
authors.lastname: Bayram
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D052160:Benzothiazoles / Q000737:chemistry*; D024841:Fluoroquinolones / Q000032:analysis*; D006835:Hydrazones / Q000737:chemistry*; D057230:Limit of Detection; D000077266:Moxifloxacin; D058451:Oxidative Coupling; D013053:Spectrophotometry / Q000379:methods; D013607:Tablets
chemical_list: D000900:Anti-Bacterial Agents; D052160:Benzothiazoles; D024841:Fluoroquinolones; D006835:Hydrazones; D013607:Tablets; C002536:3-methyl-2-benzothiazolone hydrazone; D000077266:Moxifloxacin
keywords: 3-Methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate (MBTH); Kinetic spectrophotometry; Moxifloxacin; Tablets