title: Antiproliferative and antioxidant properties of anthocyanin rich extracts from blueberry and blackcurrant juice.
abstract: The present study was aimed at evaluating the antiproliferative potential of anthocyanin-rich fractions (ARFs) obtained from two commercially available juices (blueberry and blackcurrant juices) on three tumor cell lines; B16F10 (murine melanoma), A2780 (ovarian cancer) and HeLa (cervical cancer). Individual anthocyanin determination, identification and quantification were done using HPLC-MS. Antioxidant activity of the juices was determined through different mechanism methods such as DPPH and ORAC. For biological testing, the juices were purified through C18 cartridges in order to obtain fractions rich in anthocyanins. The major anthocyanins identified were glycosylated cyanidin derivatives. The antiproliferative activity of the fractions was tested using the MTT assay. The antiproliferative potential of ARF was found to be associated with those bioactive molecules, anthocyanins due to their antioxidant potential. The results obtained indicated that both blueberry and blackcurrants are rich sources of antioxidants including anthocyanins and therefore these fruits are highly recommended for daily consumption to prevent numerous degenerative diseases.
authors.affiliation: Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. zorita.sconta@usamvcluj.ro.
authors.forename: Zoriţa
authors.initials: Z
authors.lastname: Diaconeasa
authors.affiliation: Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. loredana.leopold@usamvcluj.ro.
authors.forename: Loredana
authors.initials: L
authors.lastname: Leopold
authors.affiliation: Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. dumitrita.rugina@usamvcluj.ro.
authors.forename: Dumitriţa
authors.initials: D
authors.lastname: Rugină
authors.affiliation: Department of Food Engineering, Canakkale Onsekiz Mart University, Canakkale 17020, Turkey. ayvaz61@yahoo.com.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Ayvaz
authors.affiliation: Faculty of Food Science and Technology, University of Agricultural Science and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, Cluj-Napoca 400372, Romania. carmen.socaciu@usamvcluj.ro.
authors.forename: Carmen
authors.initials: C
authors.lastname: Socaciu
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001628:Beverages / Q000032:analysis*; D029798:Blueberry Plants / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D019073:HT29 Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins; D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D010936:Plant Extracts