title: Synthesis and evaluation of a new Rhodamine B and Di(2-picolyl)amine conjugate as a highly sensitive and selective chemosensor for Al3+ and its application in living-cell imaging.
abstract: A new Rhodamine B derivative (RBDPA), namely, N(1)-(2-(3',6'-bis(diethylamino)-3-oxospiro[isoindoline-1,9'-xanthen]-2-yl)ethyl)-N(4),N(4)-bis(pyridin-2-ylmethyl)succinamide, was designed, synthesized and structurally characterized to develop a chemosensor. The studies show that RBDPA exhibits high sensitivity and selectivity toward Al(3+) among many other metal cations in an ethanol/H2O (1:1, v/v, pH=7.2, HEPES buffer, 0.1mM) solution. Fluorescence microscopy experiments further demonstrate that RBDPA can be used as a fluorescent probe to detect Al(3+) in living cells.
authors.affiliation: Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China. Electronic address: baoxiaofeng@mail.njust.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Bao
authors.affiliation: Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
authors.forename: Qiansheng
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Jiangsu Key Laboratory of Drug Screen, New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yazhou
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Drug Screen, New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yuanxue
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of Drug Screen, New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Chemistry, School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Chemistry, School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
authors.forename: Baojing
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of Drug Screen, New Drug Screening Center, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Pang
authors.affiliation: Department of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, PR China. Electronic address: zhujing1227@hotmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000535:Aluminum / Q000032:analysis*; D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry*; D002412:Cations / Q000032:analysis; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000166:cytology; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D061848:Optical Imaging; D010848:Picolinic Acids / Q000138:chemical synthesis / Q000737:chemistry*; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: C501847:2,2'-dipicolylamine; D000588:Amines; D002412:Cations; D005456:Fluorescent Dyes; D010848:Picolinic Acids; D012235:Rhodamines; D000535:Aluminum; C029773:rhodamine B
keywords: Al(3+); Cell imaging; Chemosensor; Fluorescent probe; Rhodamine B derivative