title: Simple sequence repeat markers for the endangered species Clianthus puniceus and C. maximus (Fabaceae).
abstract: OBJECTIVE
  Microsatellite markers were developed for Clianthus puniceus using a shotgun sequencing library and tested for cross amplification in the closely related C. maximus to inform population management of these two endangered species. •
  
  
  RESULTS
  We constructed a shotgun sequencing library using a Roche 454 sequencer and searched the resulting data set for putative microsatellite regions. We optimized 12 of these regions to produce polymorphic markers for Clianthus. We tested these markers on four populations of C. maximus and on four C. puniceus individuals of known provenance. Alleles per locus ranged from two to nine, while observed and expected heterozygosities per locus ranged from 0.000 to 1.000 and 0.178 to 0.600, respectively. •
  
  
  CONCLUSIONS
  These markers will be valuable for ongoing monitoring of the genetic variation in naturally occurring populations of Clianthus and for the selection of individuals for revegetation projects in the species' former range.
authors.affiliation: Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Houliston
authors.affiliation: Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Ana
authors.initials: A
authors.lastname: Ramón-Laca
authors.affiliation: Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Reema
authors.initials: R
authors.lastname: Jain
authors.affiliation: Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Caroline M
authors.initials: CM
authors.lastname: Mitchell
authors.affiliation: Landcare Research, Gerald Street, Lincoln 7640, New Zealand.
authors.forename: Dagmar F
authors.initials: DF
authors.lastname: Goeke
keywords: Clianthus maximus; Clianthus puniceus; Fabaceae; simple sequence repeats (SSRs)