title: Simultaneous determination of isoflavones and resveratrols for adulteration detection of soybean and peanut oils by mixed-mode SPE LC-MS/MS.
abstract: To ensure authenticity of vegetable oils, isoflavones (genistein, genistin, daidzein and daidzin) and resveratrols (cis-resveratrol and trans-resveratrol) were selected as the putative markers for adulteration of soybean and peanut oils. Firstly, mixed mode solid-phase extraction coupled with liquid chromatography tandem mass spectrometry (mixed-mode SPE LC-MS/MS) method was developed to analyze isoflavones and resveratrols in vegetable oils. The concentration of marker compounds in vegetable oils were 0.08-1.47mgkg(-1) for daidzein, ND-78.9μgkg(-1) for daidzin, 0.40-5.89mgkg(-1) for genistein, 1.2-114.9μgkg(-1) for genistin, 3.1-85.0μgkg(-1) for trans-resveratrol and 1.9-51.0μgkg(-1) for cis-resveratrol, which are compatible with the raw materials for oil press. Additionally, the applicability of this method has been successfully tested in thirteen vegetable oils from the market. Mixed-mode SPE LC-MS/MS method can simultaneously detect isoflavones and resveratrols in vegetable oils and assess adulteration and quality of soybean and peanut oils.
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: mafeicpu@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China. Electronic address: peiwuli@oilcrops.cn.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Li
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Liangxiao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China.
authors.forename: Xiupin
authors.initials: X
authors.lastname: Wang
mesh_terms: D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination; D007529:Isoflavones / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D010938:Plant Oils / Q000737:chemistry*; D000077185:Resveratrol; D052616:Solid Phase Extraction / Q000379:methods*; D013025:Glycine max / Q000737:chemistry*; D013267:Stilbenes / Q000737:chemistry*
chemical_list: D007529:Isoflavones; D010938:Plant Oils; D013267:Stilbenes; D000077185:Resveratrol
keywords: Adulteration; Daidzein (Pubchem CID: 5281708); Daidzin (Pubchem CID: 107971); Genistein (Pubchem CID: 5280961); Genistin (Pubchem CID: 5281377); Isoflavones; Liquid chromatography–tandem mass spectrometry (LC–MS/MS); Mixed-mode solid-phase extraction (SPE); Resveratrols; cis-Resveratrol (Pubchem CID: 1548910); trans-Resveratrol (Pubchem CID: 445154)