title: Production of bioactive peptide hydrolysates from deer, sheep and pig plasma using plant and fungal protease preparations.
abstract: Plasma separated from deer, sheep and pig blood, obtained from abattoirs, was hydrolysed using protease preparations from plant (papain and bromelain) and fungal (FP400 and FPII) sources. Antioxidant and antimicrobial activities of the peptide hydrolysates obtained after 1, 2, 4 and 24h of hydrolysis, were investigated. The release of trichloroacetic acid-soluble peptides over the hydrolysis period was monitored using the o-phthaldialdehyde (OPA) assay, while the hydrolysis profiles were visualised using SDS-PAGE. The major plasma proteins in the animal plasmas were identified using MALDI-TOF-TOF MS. Hydrolysates of plasma generated with fungal proteases exhibited higher DPPH radical-scavenging, oxygen radical-scavenging capacity (ORAC) and ferric reducing antioxidant power (FRAP) than those generated with plant proteases for all three animal plasmas. No antimicrobial activity was detected in the hydrolysates. The results indicated that proteolytic hydrolysis of animal blood plasmas, using fungal protease preparations in particular, produces hydrolysates with high antioxidant properties.
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand. Electronic address: clara.bah@otago.ac.nz.
authors.forename: Clara S F
authors.initials: CS
authors.lastname: Bah
authors.affiliation: Department of Food Science, University of Otago, Dunedin, New Zealand.
authors.forename: Alaa El-Din A
authors.initials: Ael-D
authors.lastname: Bekhit
authors.affiliation: Department of Biochemistry, University of Otago, Dunedin, New Zealand.
authors.forename: Alan
authors.initials: A
authors.lastname: Carne
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Michelle A
authors.initials: MA
authors.lastname: McConnell
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D003670:Deer / Q000097:blood*; D005658:Fungi / Q000201:enzymology*; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000737:chemistry*; D010944:Plants / Q000201:enzymology; D011492:Protein Hydrolysates / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D012756:Sheep / Q000097:blood*; D013552:Swine / Q000097:blood*
chemical_list: D000975:Antioxidants; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases
keywords: Animal blood plasma; Antioxidant activity; Fungal protease; Hydrolysis; Plant protease