title: Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting.
abstract: Inspired by microvesicle-mediated intercellular communication, we propose a hybrid vector for magnetic drug delivery. It consists of macrophage-derived microvesicles engineered to enclose different therapeutic agents together with iron oxide nanoparticles. Here, we investigated in vitro how magnetic nanoparticles may influence the vector effectiveness in terms of drug uptake and targeting. Human macrophages were loaded with iron oxide nanoparticles and different therapeutic agents: a chemotherapeutic agent (doxorubicin), tissue-plasminogen activator (t-PA) and two photosensitizers (disulfonated tetraphenyl chlorin-TPCS2a and 5,10,15,20-tetra(m-hydroxyphenyl)chlorin-mTHPC). The hybrid cell microvesicles were magnetically responsive, readily manipulated by magnetic forces and MRI-detectable. Using photosensitizer-loaded vesicles, we showed that the uptake of microvesicles by cancer cells could be kinetically modulated and spatially controlled under magnetic field and that cancer cell death was enhanced by the magnetic targeting. From the clinical editor: In this article, the authors devised a biogenic method using macrophages to produce microvesicles containing both iron oxide and chemotherapeutic agents. They showed that the microvesicles could be manipulated by magnetic force for targeting and subsequent delivery of the drug payload against cancer cells. This smart method could provide a novel way for future fight against cancer.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, France; Inserm, U1148, Cardiovascular Bio-Engineering, X. Bichat Hospital, Université Paris Diderot Paris, France; Université Paris 13, Sorbonne Paris Cité, France.
authors.forename: Amanda K A
authors.initials: AK
authors.lastname: Silva
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Luciani
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, France.
authors.forename: Kelly
authors.initials: K
authors.lastname: Aubertin
authors.affiliation: Laboratoire Jean Perrin-CNRS FRE3231, Université Pierre et Marie Curie-Paris 6, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Bonneau
authors.affiliation: Inserm, U1148, Cardiovascular Bio-Engineering, X. Bichat Hospital, Université Paris Diderot Paris, France; Université Paris 13, Sorbonne Paris Cité, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Chauvierre
authors.affiliation: Inserm, U1148, Cardiovascular Bio-Engineering, X. Bichat Hospital, Université Paris Diderot Paris, France; Université Paris 13, Sorbonne Paris Cité, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot, France. Electronic address: claire.wilhelm@univ-paris-diderot.fr.
authors.forename: Claire
authors.initials: C
authors.lastname: Wilhelm
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000903:Antibiotics, Antineoplastic; D058185:Magnetite Nanoparticles; D004317:Doxorubicin
keywords: Cell microvesicles; Drug targeting; Magnetic manipulation; Magnetic nanoparticles; Magnetofection