title: Inubosins A, B, and C are acridine alkaloids isolated from a culture of Streptomyces sp. IFM 11440 with Ngn2 promoter activity.
abstract: Three new acridine alkaloids, inubosins A (1), B (2), and C (3), were isolated from an extract of a culture of Streptomyces sp. IFM 11440 using bioassay-guided fractionation. Neurogenin2 (Ngn2) is an activator-type basic helix-loop-helix transcription factor that promotes neural stem cell differentiation. Using cell-based Ngn2 promoter activity-guided screening, Streptomyces sp. IFM 11440 was found to induce Ngn2 promoter activity. The structures of 1-3 were established using spectroscopic methods, including 1D- and 2D-NMR measurements. Inubosin B (2) showed potent Ngn2 promoter activity. Moreover, inubosin B (2) increased mRNA expression of genes related to neural stem cell differentiation.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Chiba University , 1-8-1 Inohana, Chuo-ku, Chiba 260-8675, Japan.
authors.forename: Midori A
authors.initials: MA
authors.lastname: Arai
authors.forename: Kazune
authors.initials: K
authors.lastname: Koryudzu
authors.forename: Masami
authors.initials: M
authors.lastname: Ishibashi
mesh_terms: D000166:Acridines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051792:Basic Helix-Loop-Helix Proteins / Q000187:drug effects*; D002454:Cell Differentiation; D007564:Japan; D015394:Molecular Structure; D009419:Nerve Tissue Proteins / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry*
chemical_list: D000166:Acridines; D000470:Alkaloids; D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; C102938:Neurog2 protein, mouse; C000600273:inubosin A; C000600274:inubosin B; C000600275:inubosin C