title: Development of gemcitabine-adsorbed magnetic gelatin nanoparticles for targeted drug delivery in lung cancer.
abstract: Magnetic iron oxide nanoparticles (IONPs) were coated with gelatin type B by means of the two-step desolvation method. Drug loading by adsorption was studied under various conditions such as different temperature, contact time, pH, and initial gemcitabine concentration. Further, Langmuir isotherm curves were constracted and constants were calculated. According to the Langmuir isotherm, the Gibbs free energy of the adsorption process at 25°C was - 4.74 kJ/mol. On the other hand, this value at 37°C was - 7.86 kJ/mol. In vitro drug release was performed at pH levels of 5 and 7.4, with gemcitabine-loaded magnetic gelatin nanoparticles and free gemcitabine, and both the results were subsequently compared.
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Senay
authors.initials: S
authors.lastname: Hamarat Sanlıer
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Yasa
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Aslı Ozge
authors.initials: AO
authors.lastname: Cihnioglu
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Abdulhayoglu
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Habibe
authors.initials: H
authors.lastname: Yılmaz
authors.affiliation: a Department of Biochemistry , Faculty of Science, Ege University , Izmir , Turkey.
authors.forename: Güliz
authors.initials: G
authors.lastname: Ak
mesh_terms: D000818:Animals; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D000093542:Gemcitabine
chemical_list: D058185:Magnetite Nanoparticles; D003841:Deoxycytidine; D005780:Gelatin; D000093542:Gemcitabine
keywords: adsorption; drug delivery; gemcitabine; isotherm; kinetic modeling; magnetic gelatin nanoparticles