title: Fourteen polymorphic microsatellite markers for the threatened Arnica montana (Asteraceae).
abstract: OBJECTIVE
  Microsatellite markers were developed to investigate population genetic structure in the threatened species Arnica montana. •
  
  
  RESULTS
  Fourteen microsatellite markers with di-, tetra-, and hexanucleotide repeat motifs were developed for A. montana using 454 pyrosequencing without and with library-enrichment methods, resulting in 56,545 sequence reads and 14,467 sequence reads, respectively. All loci showed a high level of polymorphism, with allele numbers ranging from four to 11 in five individuals from five populations (25 samples) and an expected heterozygosity ranging from 0.192 to 0.648 across the loci. •
  
  
  CONCLUSIONS
  This set of microsatellite markers is the first one described for A. montana and will facilitate conservation genetic applications as well as the understanding of phylogeographic patterns in this species.
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany.
authors.forename: Virginia K
authors.initials: VK
authors.lastname: Duwe
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany.
authors.forename: Sascha A
authors.initials: SA
authors.lastname: Ismail
authors.affiliation: Ecogenics GmbH, Grabenstraße 11a, Schlieren, 8952 Zurich, Switzerland.
authors.forename: Andres
authors.initials: A
authors.lastname: Buser
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany.
authors.forename: Esther
authors.initials: E
authors.lastname: Sossai
authors.affiliation: Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195 Berlin, Germany ; Institut für Biologie-Botanik, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Altensteinstraße 6, 14195 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Borsch
authors.affiliation: Institut für Biologie-Botanik, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Altensteinstraße 6, 14195 Berlin, Germany.
authors.forename: Ludo A H
authors.initials: LA
authors.lastname: Muller
keywords: Arnica montana; microsatellites; population genetics; pyrosequencing