title: Production of lactate and acetate by Lactobacillus coryniformis subsp. torquens DSM 20004(T) in comparison with Lactobacillus amylovorus DSM 20531(T).
abstract: Lactobacillus coryniformis subsp. torquens DSM20004(T) is a d-lactate producer, with a portion of the d-lactate higher than 99.9% of total lactic acid produced. Acetate was identified as the second end-product that appeared at the end of the exponential growth phase in MRS medium when glucose concentration dropped to 38.41mM (6.92g/L). The acetate production was prolonged to the stationary phase, while the concentration of d-lactate remained constant. Other end-products were not identified by HPLC method. The known metabolic pathways of glucose fermentation in lactic acid bacteria do not produce the particular combination of these two end-products, but besides lactate and acetate also formate, ethanol and CO2 are produced. For comparison, the production of lactate and acetate by a d-/l-lactate producer Lactobacillus amylovorus DSM 20531(T) was also investigated. This strain produced equimolar quantities of d- and l-lactate in the MRS medium. Acetate was produced only when initial concentration of glucose was 55.51mM (10g/L) and production started in the exponential phase when concentration of glucose dropped to 35.52mM (6.40g/L). Similar behavior was observed with the initial concentration of maltose of 29.21mM (10g/L). An unstructured mathematical model was established for the bioprocess simulation.
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia. Electronic address: aslavica@pbf.hr.
authors.forename: Anita
authors.initials: A
authors.lastname: Slavica
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia.
authors.forename: Antonija
authors.initials: A
authors.lastname: Trontel
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia.
authors.forename: Nuša
authors.initials: N
authors.lastname: Jelovac
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia.
authors.forename: Željka
authors.initials: Ž
authors.lastname: Kosovec
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia.
authors.forename: Božidar
authors.initials: B
authors.lastname: Šantek
authors.affiliation: University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory of Biochemical Engineering, Industrial Microbiology, Malting and Brewing Technology, Zagreb, Croatia.
authors.forename: Srđan
authors.initials: S
authors.lastname: Novak
mesh_terms: D019342:Acetic Acid / Q000302:isolation & purification / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003470:Culture Media / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D019344:Lactic Acid / Q000302:isolation & purification / Q000378:metabolism*; D007778:Lactobacillus / Q000737:chemistry / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D008320:Maltose / Q000378:metabolism; D008954:Models, Biological
chemical_list: D003470:Culture Media; D019344:Lactic Acid; D008320:Maltose; D005947:Glucose; D019342:Acetic Acid
keywords: Acetate production; Glucose limitation; Lactobacillus amylovorus; Lactobacillus coryniformis; d-lactate production