title: A sequential statistical approach towards an optimized production of a broad spectrum bacteriocin substance from a soil bacterium Bacillus sp. YAS 1 strain.
abstract: Bacteriocins, ribosomally synthesized antimicrobial peptides, display potential applications in agriculture, medicine, and industry. The present study highlights integral statistical optimization and partial characterization of a bacteriocin substance from a soil bacterium taxonomically affiliated as Bacillus sp. YAS 1 after biochemical and molecular identifications. A sequential statistical approach (Plackett-Burman and Box-Behnken) was employed to optimize bacteriocin (BAC YAS 1) production. Using optimal levels of three key determinants (yeast extract (0.48% (w/v), incubation time (62 hrs), and agitation speed (207 rpm)) in peptone yeast beef based production medium resulted in 1.6-fold enhancement in BAC YAS 1 level (470 AU/mL arbitrary units against Erwinia amylovora). BAC YAS 1 showed activity over a wide range of pH (1-13) and temperature (45-80 °C). A wide spectrum antimicrobial activity of BAC YAS 1 against the human pathogens (Clostridium perfringens, Staphylococcus epidermidis, Campylobacter jejuni, Enterobacter aerogenes, Enterococcus sp., Proteus sp., Klebsiella sp., and Salmonella typhimurium), the plant pathogen (E. amylovora), and the food spoiler (Listeria innocua) was demonstrated. On top and above, BAC YAS 1 showed no antimicrobial activity towards lactic acid bacteria (Lactobacillus bulgaricus, L. casei, L. lactis, and L. reuteri). Promising characteristics of BAC YAS 1 prompt its commercialization for efficient utilization in several industries.
authors.affiliation: Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, Egypt.
authors.forename: Amira M
authors.initials: AM
authors.lastname: Embaby
authors.affiliation: Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, Egypt.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Heshmat
authors.affiliation: Department of Biotechnology, Institute of Graduate Studies and Research, University of Alexandria, 163 Horreya Avenue, P.O. Box 832, Chatby 21526, Egypt.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Hussein
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, University of Alexandria, P.O. Box 832, Chatby 21526, Egypt ; University of Alberta, Department of Civil and Environmental Engineering, Edmonton, AB, Canada.
authors.forename: Heba S
authors.initials: HS
authors.lastname: Marey
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D003016:Clostridium perfringens / Q000187:drug effects; D006801:Humans; D012988:Soil Microbiology
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001430:Bacteriocins