title: Microbial keratitis in los angeles: the doheny eye institute and the los angeles county hospital experience.
abstract: OBJECTIVE
  To evaluate the spectrum and antibiotic susceptibility panel of infectious keratitis at a major tertiary care referral eye center and a major county hospital in Southern California.
  
  
  METHODS
  Retrospective case series.
  
  
  METHODS
  All cultured infectious keratitis cases from July 1, 2008, through December 31, 2012, from the Doheny Eye Institute (DEI) and the Los Angeles County + University of Southern California Medical Center (LAC+USC) were evaluated.
  
  
  METHODS
  Microbiology records were reviewed retrospectively.
  
  
  METHODS
  Microbial isolates as well as antibiotic susceptibility patterns were analyzed.
  
  
  RESULTS
  One hundred eighty-four (63%) of 290 cases showed positive culture results at DEI and 152 (82%) of 186 cases showed positive culture results at LAC+USC. Gram-positive pathogens were found to be the most common at both DEI (70%) and LAC+USC (68%), with coagulase-negative Staphylococcus being the most common gram-positive organism (58% at DEI and 44% at LAC+USC). Pseudomonas aeruginosa was the most common gram-negative organism (57% at DEI and 43% at LAC+USC). Ciprofloxacin and levofloxacin susceptibility for all tested pathogens was 73% at DEI and 81% at LAC+USC (P = 0.16). Oxacillin-resistant Staphylococcus aureus (ORSA) was found in 42% of cases at DEI and in 45% of cases at LAC+USC (P = 1.00).
  
  
  CONCLUSIONS
  There is no significant difference in the spectrum of pathogens or antibiotic susceptibility of pathogens at DEI versus LAC+USC, and ORSA was found in approximately half of all S. aureus samples.
authors.affiliation: Department of Ophthalmology, Los Angeles County - University of Southern California Medical Center, Los Angeles, California.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sand
authors.affiliation: Department of Pathology, Keck Medical Center of the University of Southern California, Los Angeles, California.
authors.forename: Rosemary
authors.initials: R
authors.lastname: She
authors.affiliation: Department of Pathology, Keck Medical Center of the University of Southern California, Los Angeles, California; Department of Pathology, Los Angeles County - University of Southern California Medical Center, Los Angeles, California.
authors.forename: Ira A
authors.initials: IA
authors.lastname: Shulman
authors.affiliation: Keck School of Medicine, University of Southern California, Los Angeles, California.
authors.forename: David S
authors.initials: DS
authors.lastname: Chen
authors.affiliation: Keck School of Medicine, University of Southern California, Los Angeles, California.
authors.forename: Mathew
authors.initials: M
authors.lastname: Schur
authors.affiliation: Doheny Eye Centers, Department of Ophthalmology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California. Electronic address: HHsu@doheny.org.
authors.forename: Hugo Y
authors.initials: HY
authors.lastname: Hsu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use; D001419:Bacteria / Q000187:drug effects; D002648:Child; D002939:Ciprofloxacin / Q000494:pharmacology / Q000627:therapeutic use; D003320:Corneal Ulcer / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D015818:Eye Infections, Bacterial / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D005260:Female; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology; D006766:Hospitals, County / Q000706:statistics & numerical data; D006801:Humans; D064704:Levofloxacin / Q000494:pharmacology / Q000627:therapeutic use; D015141:Los Angeles / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D012189:Retrospective Studies
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D064704:Levofloxacin