title: Immobilization of Aspergillus niger F7-02 Lipase in Polysaccharide Hydrogel Beads of Irvingia gabonensis Matrix.
abstract: The potential of polysaccharide Irvingia gabonensis matrix as enzyme immobilization support was investigated. Lipase of Aspergillus niger F7-02 was immobilized by entrapment using glutaraldehyde as the cross-linking agent and stabilized in ethanolic-formaldehyde solution. The pH and temperature stability and activity yield of the immobilized enzyme were determined. Such parameters as enzyme load, bead size, number of beads, and bead reusability were also optimized. Adequate gel strength to form stabilized beads was achieved at 15.52% (w/v) Irvingia gabonensis powder, 15% (v/v) partially purified lipase, 2.5% (v/v) glutaraldehyde, and 3 : 1 (v/v) ethanolic-formaldehyde solution. There was 3.93-fold purification when the crude enzyme was partially purified in two-step purification using Imarsil and activated charcoal. Optimum lipase activity 75.3 Ug(-1) was achieved in 50 mL test solution containing 15 beads of 7 mm bead size. Relative activity 80% was retained at eight repeated cycles. The immobilization process gave activity yield of 59.1% with specific activity of 12.3 Umg(-1) and stabilized at optimum pH 4.5 and temperature 55°C. Thus the effectiveness and cost-efficiency of I. gabonensis as a polymer matrix for lipase immobilization have been established.
authors.affiliation: Department of Microbiology, Federal University of Agriculture, PMB 2240, Abeokuta 110001, Ogun State, Nigeria.
authors.forename: Safaradeen Olateju
authors.initials: SO
authors.lastname: Kareem
authors.affiliation: Department of Microbiology, Federal University of Agriculture, PMB 2240, Abeokuta 110001, Ogun State, Nigeria.
authors.forename: Olayinka Quadri
authors.initials: OQ
authors.lastname: Adio
authors.affiliation: Department of Biological Sciences, McPherson University, Seriki-Sotayo, PMB 2094, Abeokuta 110116, Ogun State, Nigeria.
authors.forename: Michael Bamitale
authors.initials: MB
authors.lastname: Osho