title: An ignored variable: solution preparation temperature in protein crystallization.
abstract: Protein crystallization is affected by many parameters, among which certain parameters have not been well controlled. The temperature at which the protein and precipitant solutions are mixed (i.e., the ambient temperature during mixing) is such a parameter that is typically not well controlled and is often ignored. In this paper, we show that this temperature can influence protein crystallization. The experimental results showed that both higher and lower mixing temperatures can enhance the success of crystallization, which follows a parabolic curve with an increasing ambient temperature. This work illustrates that the crystallization solution preparation temperature is also an important parameter for protein crystallization. Uncontrolled or poorly controlled room temperature may yield poor reproducibility in protein crystallization.
authors.affiliation: 1] Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China [2] Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi, People's Republic of China.
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Chen
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Lu
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Qing-Di
authors.initials: QD
authors.lastname: Cheng
authors.affiliation: Advanced Materials Test Center, School of Mechanics, Civil Engineering and Architecture, Northwestern Polytechnical University, Xi'an 710129, Shaanxi, People's Republic of China.
authors.forename: Liang-Bo
authors.initials: LB
authors.lastname: Ao
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Chen-Yan
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Hai
authors.initials: H
authors.lastname: Hou
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Liu
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Li
authors.affiliation: Institute for Special Environmental Biophysics, Key Lab for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
authors.forename: Da-Chuan
authors.initials: DC
authors.lastname: Yin
mesh_terms: D003460:Crystallization; D019286:Endopeptidase K / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D012996:Solutions / Q000737:chemistry; D013696:Temperature
chemical_list: D010940:Plant Proteins; D011506:Proteins; D012996:Solutions; C003427:thaumatin protein, plant; D009113:Muramidase; D019286:Endopeptidase K