title: Adverse effects of coal combustion related fine particulate matter (PM2.5) on nematode Caenorhabditis elegans.
abstract: The toxic effects of coal combustion related fine particulate matter (PM2.5), collected from Datong, Shanxi province, China, on nematode Caenorhabditis elegans were investigated. Exposure to PM2.5 resulted in deficits in development, reproduction, locomotion behavior, and lifespan, and induction of intestinal autofluorescence or reactive oxygen species (ROS) production. Prolonged exposure to PM2.5 led to more severe toxicity on nematodes than acute exposure. In addition, exposure to PM2.5 induced altered expression patterns of genes required for the control of oxidative stress. Reduction in mean defecation cycle length and developmental deficits in AVL and DVB neurons, which are involved in the control of defecation behavior, were also triggered by PM2.5 exposure. Thus, oxidative stress and abnormal defecation behavior may contribute greatly to the toxicity of coal combustion related PM2.5 in nematodes. The results also imply that the long-term adverse effects of coal combustion related PM2.5 on environmental organisms should be carefully considered.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
authors.forename: Lingmei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control Technology for Environmental & Food Safety, Tianjin 300050, China.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liao
authors.affiliation: Institute of Health and Environmental Medicine, Key Laboratory of Risk Assessment and Control Technology for Environmental & Food Safety, Tianjin 300050, China. Electronic address: zhugexi2003@sina.com.
authors.forename: Zhuge
authors.initials: Z
authors.lastname: Xi
authors.affiliation: Key Laboratory of Environmental Medicine Engineering in Ministry of Education, Medical School of Southeast University, Nanjing 210009, China. Electronic address: dayongw@seu.edu.cn.
authors.forename: Dayong
authors.initials: D
authors.lastname: Wang
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D002681:China; D003031:Coal / Q000633:toxicity*; D007422:Intestines; D008124:Locomotion / Q000187:drug effects; D018384:Oxidative Stress; D052638:Particulate Matter / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012098:Reproduction
chemical_list: D000393:Air Pollutants; D003031:Coal; D052638:Particulate Matter; D017382:Reactive Oxygen Species
keywords: Caenorhabditis elegans; Coal combustion related PM(2.5); Defecation behavior; Oxidative stress; Toxicity assessment