title: Investigations of blue light-induced reactive oxygen species from flavin mononucleotide on inactivation of E. coli.
abstract: The micronutrients in many cellular processes, riboflavin, flavin mononucleotide (FMN), and flavin adenine dinucleotide (FAD) are photo-sensitive to UV and visible light for generating reactive oxygen species (ROS). Produced from phosphorylation of riboflavin, FMN is more water-soluble and rapidly transformed into free riboflavin after ingestion. This study investigated the application of visible blue light with FMN to development of an effective antimicrobial treatment. The photosensitization of bacterial viability with FMN was investigated by light quality, intensity, time, and irradiation dosage. The blue light-induced photochemical reaction with FMN could inactivate Escherichiacoli by the generated ROS in damaging nucleic acids, which was validated. This novel photodynamic technique could be a safe practice for photo-induced inactivation of environmental microorganism to achieve hygienic requirements in food processing.
authors.affiliation: Department of Biotechnology, Ming-Chuan University, Taoyüan 33343, Taiwan.
authors.forename: Ji-Yuan
authors.initials: JY
authors.lastname: Liang
authors.affiliation: Department of Biotechnology, Ming-Chuan University, Taoyüan 33343, Taiwan; Department of Restaurant and Institutional Management, Shih-Chien University, Taipei 10462, Taiwan.
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Cheng
authors.affiliation: Department of Biotechnology, Ming-Chuan University, Taoyüan 33343, Taiwan.
authors.forename: Chin-Hao
authors.initials: CH
authors.lastname: Yu
authors.affiliation: Department of Biotechnology, Ming-Chuan University, Taoyüan 33343, Taiwan. Electronic address: loknath@mail.mcu.edu.tw.
authors.forename: Liang-Yü
authors.initials: LY
authors.lastname: Chen
mesh_terms: D003116:Color; D004269:DNA, Bacterial / Q000737:chemistry; D004278:DNA, Superhelical / Q000737:chemistry; D003666:Decontamination; D003855:Deoxyribose / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology* / Q000528:radiation effects*; D005486:Flavin Mononucleotide / Q000378:metabolism*; D008027:Light; D050296:Microbial Viability / Q000528:radiation effects*; D010782:Photolysis; D010957:Plasmids / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D004278:DNA, Superhelical; D017382:Reactive Oxygen Species; D003855:Deoxyribose; D005486:Flavin Mononucleotide