title: Previous exposure to Cyclosporine A and periodontal breakdown in rats.
abstract: OBJECTIVE
  The aim of this study was to evaluate the influence of previous exposure to Cyclosporine A (CsA) on experimental periodontitis in rats.
  
  
  METHODS
  Forty rats were divided into 4 groups: Control (CON); Cyclosporine A (CsA), which received daily doses of 10mg/kg CsA; Ligature (LIG), which received an insertion of a cotton ligature around the mandibular 1st molar at day 30; and Ligature and CsA (CsAL), which were treated with CsA and the cotton ligature. At day 60 of the experiment, animals were sacrificed, and groups were compared with regards to Alkaline Phosphatase (AP) activity, gingival overgrowth, periodontal bone support (PBS), bone resorption at furcation ligament area (LA) and TRAP+ cells. Data were analyzed by ANOVA/Tukey and Kruskal-Wallis and were considered to be statistically significant at 5% level.
  
  
  RESULTS
  CsA and LIG groups showed similar gingival area, which was higher than that in the CON and lower than in the CsAL group (p=0.001). The ratio between epithelial area and connective area for the CON group was similar to the CsA group and higher than that for the CsAL and LIG groups (p=0.0334). Mean percentage of PBS for the CON group was similar to that for the CsAL group and higher than that of the CsA and LIG groups (p=0.0007). No difference was observed regarding AP (p=0.2806) and TRAP+ cells (p=0.3995) among experimental groups. Mean values for LA of CON were similar to CsA, and both were statistically lower than the CsAL and LIG groups (p=0.0172).
  
  
  CONCLUSIONS
  Based on these results, we posit that previous exposure to CsA may influence gingival overgrowth, but not bone loss, in rats with experimental periodontitis.
authors.affiliation: University of Taubaté, Rua dos Operários, 09 Taubaté, SP, Brazil. Electronic address: felipe.periodontia@hotmail.com.
authors.forename: Felipe
authors.initials: F
authors.lastname: da Silva Peralta
authors.affiliation: Implantology Department, University of Santo Amaro - UNISA, R. Prof. Enéas de Siqueira Neto, 312 - São Paulo, SP, Brazil; Periodontics Department, Dentistry Department of University of Taubaté, Taubaté, SP, Brazil. Electronic address: dpallos@netpoint.com.br.
authors.forename: Debora
authors.initials: D
authors.lastname: Pallos
authors.affiliation: Dentistry Departament of University Veiga de Almeida - UVA, Rua Ibituruna, 108 - Maracanã, Rio de Janeiro - RJ 20271-020, Brazil. Electronic address: celsoq@yahoo.com.
authors.forename: Celso
authors.initials: C
authors.lastname: Silva Queiroz
authors.affiliation: Private office, Pindamonhangaba, SP, Brazil. Electronic address: lumello1@uol.com.br.
authors.forename: Lucilene Hernandes
authors.initials: LH
authors.lastname: Ricardo
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D016301:Alveolar Bone Loss / Q000000981:diagnostic imaging; D000818:Animals; D001862:Bone Resorption / Q000000981:diagnostic imaging; D016572:Cyclosporine / Q000633:toxicity*; D004195:Disease Models, Animal; D019214:Gingival Overgrowth / Q000139:chemically induced*; D007527:Isoenzymes / Q000378:metabolism; D008026:Ligation; D008297:Male; D008334:Mandible; D008963:Molar; D010518:Periodontitis / Q000378:metabolism*; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D016572:Cyclosporine; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
keywords: Alveolar bone loss; Animal experimentation; Cyclosporine A; Gingival overgrowth; Osteoporosis