title: Prediction of the zeta potentials and ionic descriptors of a silica hydride stationary phase with mobile phases of different pH and ionic strength.
abstract: In this study, the zeta potentials of a silica hydride stationary phase (Diamond Hydride™) in the presence of different water-acetonitrile mixtures (from 0-80% (v/v) acetonitrile) of different ionic strengths (from 0-40mM) and pH values (from pH 3.0-7.0) have been investigated. Debye-Hückel theory was applied to explain the effect of changes in the pH and ionic strength of these aqueous media on the negative zeta potential of this stationary phase. The experimental zeta potentials of the Diamond Hydride™ particles as a function of acetonitrile content up to 50% (v/v) correlated (R(2)=0.998) with the predicted zeta potential values based on this established theory, when the values of the dissociation constant of all related species, as well as viscosity, dielectric constant and refractive index of the aqueous medium were taken into consideration. Further, the retention behavior of basic, acidic and neutral analytes was investigated under mobile phase conditions of higher pH and lower ionic strength. Under these conditions, the Diamond Hydride™ stationary phase surface became more negative, as assessed from the increasingly more negative zeta potentials, resulting in the ion exchange characteristics becoming more dominant and the basic analytes showing increasing retention. Ionic descriptors were derived from these chromatographic experiments based on the assumption that linear solvation energy relationships prevail. The results were compared with predicted ionic descriptors based on the different calculated zeta potential values resulting in an overall correlation of R(2)=0.888. These studies provide fundamental insights into the impact on the separation performance of changes in the zeta potential of the Diamond Hydride™ surface with the results relevant to other silica hydride and, potentially, to other types of stationary phase materials.
authors.affiliation: Australian Research Council Special Research Centre for Green Chemistry, School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors.forename: Chadin
authors.initials: C
authors.lastname: Kulsing
authors.affiliation: Australian Research Council Special Research Centre for Green Chemistry, School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.affiliation: Australian Research Council Special Research Centre for Green Chemistry, School of Chemistry, Monash University, Melbourne, VIC 3800, Australia.
authors.forename: Reinhard I
authors.initials: RI
authors.lastname: Boysen
authors.affiliation: Australian Research Council Special Research Centre for Green Chemistry, School of Chemistry, Monash University, Melbourne, VIC 3800, Australia. Electronic address: milton.hearn@monash.edu.
authors.forename: Milton T W
authors.initials: MT
authors.lastname: Hearn
mesh_terms: D000097:Acetonitriles / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007477:Ions / Q000737:chemistry*; D008956:Models, Chemical; D009994:Osmolar Concentration; D017640:Silicates / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D000097:Acetonitriles; D007477:Ions; D017640:Silicates; C487012:silica hydride; D014867:Water; C032159:acetonitrile
keywords: Aqueous normal phase chromatography; Debye–Hückel theory; Ionic descriptors; Linear solvation energy relationships; Silica hydride stationary phases; Zeta potential measurements