title: [Effect of repeated hypoxic preconditioning on renal ischemia-reperfusion-induced hepatic dysfunction in rats].
abstract: OBJECTIVE
  To explore the effect of repeated hypoxic preconditioning (RHP) on renal ischemia-reperfusion-induced hepatic dysfunction in rats and the underlying mechanism.
  
  
  METHODS
  A total of 120 normal SD rats were randomly divided into 4 groups (n=40), namely RHP surgical group, RHP sham-operated (RHPS) group, nonhypoxic surgical group (IRI group), and nonhypoxic sham-operated group (S group). The rats in the hypoxic groups were exposed to hypoxia in a hypoxic chamber for 5 days prior to establishment of renal ischemia-reperfusion model by resection of the right kidney and clamping the left renal hilum. Serum alanine aminotransferase (ALT), IL-17 A, TNF-a, liver superoxide dismutase (SOD) and nitric oxide (NO) were detected at 2, 8 and 24h after reperfusion, and Western blotting was used to determine the expression of p-PI3K and p-AKT;HE staining was used to observe the structural changes in the liver.
  
  
  RESULTS
  Compared with IRI group, RHP group showed significantly milder hepatic damage, lower ALT levels and higher NO levels at 2, 8, and 24 after reperfusion (P<0.05); TNF-a levels were lowered at 24 h (P<0.05) and SOD increased at 8 h after the reperfusion (P<0.05). Compared with S group, IRI group and RHP group showed significantly higher IL-17A levels (P<0.05) but without significant difference between the latter two groups (P>0.05). The expressions of p-PI3K and P-Al<t in RHP group were significantly higher than those in IRI group (P<0.05), especially at 8 h after reperfusion (P<0.05).
  
  
  CONCLUSIONS
  Repeated hypoxic preconditioning can attenuate hepatic injury induced by renal ischemia-reperfusion injury in rats.
authors.affiliation: Anesthesia and Operation Center, Chinese PLA General Hospital, Beijing 100853, China. yanna871106@163.com
authors.forename: Na
authors.initials: N
authors.lastname: Yan
authors.forename: Ze-Guo
authors.initials: ZG
authors.lastname: Feng
authors.forename: Guang-Tao
authors.initials: GT
authors.lastname: Yan
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Yue
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Geng
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000860:Hypoxia; D020381:Interleukin-17 / Q000097:blood; D019194:Ischemic Preconditioning; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000503:physiopathology; D008099:Liver / Q000503:physiopathology*; D009569:Nitric Oxide / Q000097:blood; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D013482:Superoxide Dismutase / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D020381:Interleukin-17; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D013482:Superoxide Dismutase; D000410:Alanine Transaminase; D019869:Phosphatidylinositol 3-Kinases