title: Import and export of bacterial protein toxins.
abstract: The paper provides a short overview of three investigated bacterial protein toxins, colicin M (Cma) of Escherichia coli, pesticin (Pst) of Yersinia pestis and hemolysin (ShlAB) of Serratia marcescens. Cma and Pst are exceptional among colicins in that they kill bacteria by degrading the murein (peptidoglycan). Both are released into the medium and bind to specific receptor proteins in the outer membrane of sensitive E. coli cells. Subsequently they are translocated into the periplasm by an energy-consuming process using the proton motive force. For transmembrane translocation the colicins unfold and refold in the periplasm. In the case of Cma the FkpA peptidyl prolyl cis-trans isomerase/chaperone is required. ShlA is secreted and activated through ShlB in the outer membrane by a type Vb secretion mechanism.
authors.affiliation: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany. Electronic address: volkmar.braun@tuebingen.mpg.de.
authors.forename: Volkmar
authors.initials: V
authors.lastname: Braun
authors.affiliation: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Helbig
authors.affiliation: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
authors.forename: Silke I
authors.initials: SI
authors.lastname: Patzer
authors.affiliation: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
authors.forename: Avijit
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: Max Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tübingen, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Römer
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D001430:Bacteriocins / Q000378:metabolism*; D003087:Colicins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D021381:Protein Transport; D012706:Serratia marcescens / Q000378:metabolism; D015010:Yersinia pestis / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001430:Bacteriocins; D003087:Colicins; D006460:Hemolysin Proteins; C089911:ShlA protein, Serratia marcescens; C107896:ShlB protein, Serratia marcescens; C011330:Pesticin
keywords: Activity; Colicin M; Export; Hemolysin; Import; Pesticin