title: Erianthus arundinaceus HSP70 (EaHSP70) overexpression increases drought and salinity tolerance in sugarcane (Saccharum spp. hybrid).
abstract: Heat shock proteins (HSPs) have a major role in stress tolerance mechanisms in plants. Our studies have shown that the expression of HSP70 is enhanced under water stress in Erianthus arundinaceus. In this paper, we evaluate the effects of overexpression of EaHSP70 driven by Port Ubi 2.3 promoter in sugarcane. The transgenic events exhibit significantly higher gene expression, cell membrane thermostability, relative water content, gas exchange parameters, chlorophyll content and photosynthetic efficiency. The overexpression of EaHSP70 transgenic sugarcane led to the upregulation of stress-related genes. The transformed sugarcane plants had better chlorophyll retention and higher germination ability than control plants under salinity stress. Our results suggest that EaHSP70 plays an important role in sugarcane acclimation to drought and salinity stresses and its potential for genetic engineering of sugarcane for drought and salt tolerance.
authors.affiliation: Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India.
authors.forename: Sruthy Maria
authors.initials: SM
authors.lastname: Augustine
authors.affiliation: Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India.
authors.forename: J Ashwin
authors.initials: JA
authors.lastname: Narayan
authors.affiliation: Indian Grass and Fodder Research Institute Regional Station, Avikanagar, Rajasthan, India.
authors.forename: Divya P
authors.initials: DP
authors.lastname: Syamaladevi
authors.affiliation: Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Appunu
authors.affiliation: Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India.
authors.forename: M
authors.initials: M
authors.lastname: Chakravarthi
authors.affiliation: Department of Rice, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Ravichandran
authors.affiliation: Sugarcane Breeding Institute (ICAR), Coimbatore, Tamil Nadu, India. Electronic address: monian_52@yahoo.com.
authors.forename: N
authors.initials: N
authors.lastname: Subramonian
mesh_terms: D055864:Droughts; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000378:metabolism / Q000502:physiology; D031786:Saccharum / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D055049:Salt Tolerance / Q000235:genetics*; D014867:Water / Q000378:metabolism*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D014867:Water
keywords: Drought tolerance; Erianthus arundinaceus; HSP70; Salinity tolerance; Sugarcane; Transformation