title: Sirt1 protects pig oocyte against in vitro aging.
abstract: Sirtuins have been widely reported to be involved in multiple biological processes. However, their function during pig oocyte aging has not been reported yet. Here, we first identify that sirt1 expression is dramatically reduced in pig in vitro-aged oocytes. Furthermore, by confocal scanning and quantitative analysis, we find the increased frequency of spindle defects and chromosome misalignment, disturbed redistribution of cortical granules and mitochondria during oocyte in vitro-aging. Importantly, these aging-associated defective phenotypes can be ameliorated through resveratrol (sirt1 activator) treatment during pig oocyte maturation, providing the evidence for the hypothesis that decreased sirt1 is one of a number of factors contributing to oocyte in vitro-aging. In summary, our data indicate a role for sirt1 in pig oocytes and uncover a striking beneficial effect of sirt1 expression on aged oocytes.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Rujun
authors.initials: R
authors.lastname: Ma
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Rui
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics* / Q000502:physiology*; D002869:Chromosome Aberrations; D003594:Cytoplasmic Granules / Q000473:pathology; D005260:Female; D015870:Gene Expression; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000473:pathology; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000172:deficiency / Q000378:metabolism / Q000502:physiology*; D008941:Spindle Apparatus / Q000473:pathology; D013267:Stilbenes / Q000494:pharmacology; D013552:Swine
chemical_list: D013267:Stilbenes; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: oocyte aging; resveratrol; sirt1