title: Expression and effects of B-lymphocyte stimulator and its receptors in T cell-mediated autoimmune arthritis.
abstract: The objective of this study was to determine the expression and effects of B-lymphocyte stimulator (BLyS) in T cell-mediated autoimmune arthritis, a rat model of human rheumatoid arthritis (RA). Rat adjuvant-induced arthritis (AA) was induced by intradermal injection of 0.1ml complete Freund's adjuvant. Arthritis was evaluated by the histopathological examination of joint ankle. The BLyS expression was detected by immunohistochemical analysis. The level of BLyS and interleukin (IL)-17 were assayed by enzyme-linked immunosorbent assay. The gene expression of BLyS and its receptors (TACI, BCMA and BAFF-R) were assessed by quantitative reverse-transcription polymerase chain reaction. The effect of BLyS on the function of T cell was investigated by transwell assay. Using an AA rat model, we detected dysregulated expression and level of BLyS and its receptors in the local joint synovium tissue, peripheral lymphoid organs (spleen) and immune cells (macrophage, dendritic cells (DC) and T cell) at the peak of inflammation. In vitro, BLyS-treated DC induced IL-17 producing T cells. Neutralization of BLyS by the TACI-Ig fusion protein attenuated these stimulating effects of BLyS. These data suggest that the overproduction of BLyS may contribute to T cell responses and may be an attractive target for control of autoimmune diseases, such as RA, that involves both T and B cells.
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China. Electronic address: changyan318@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Sun
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China.
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Jia
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei 230032, China. Electronic address: wwei@ahmu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000473:pathology; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology* / Q000473:pathology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology* / Q000473:pathology; D053264:B-Cell Activating Factor / Q000097:blood / Q000235:genetics / Q000276:immunology*; D053265:B-Cell Activation Factor Receptor / Q000235:genetics; D053301:B-Cell Maturation Antigen / Q000235:genetics; D003713:Dendritic Cells / Q000276:immunology; D015870:Gene Expression; D020381:Interleukin-17 / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000276:immunology; D008297:Male; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D053303:Transmembrane Activator and CAML Interactor Protein / Q000235:genetics
chemical_list: D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; D053301:B-Cell Maturation Antigen; D020381:Interleukin-17; D053303:Transmembrane Activator and CAML Interactor Protein
keywords: Adjuvant-induced arthritis; B-lymphocyte stimulator; Receptor; Rheumatoid arthritis; T cell