title: Induction of a proton gradient across a gold-supported biomimetic membrane by electroenzymatic H2 oxidation.
abstract: Energy-transduction mechanisms in living organisms, such as photosynthesis and respiration, store light and chemical energy in the form of an electrochemical gradient created across a lipid bilayer. Herein we show that the proton concentration at an electrode/phospholipid-bilayer interface can be controlled and monitored electrochemically by immobilizing a membrane-bound hydrogenase. Thus, the energy derived from the electroenzymatic oxidation of H2 can be used to generate a proton gradient across the supported biomimetic membrane.
authors.affiliation: Instituto de Catáisis y Petroleoquímica, CSIC c/ Marie Curie 2, 28049 Madrid (Spain).
authors.forename: Óscar
authors.initials: Ó
authors.lastname: Gutiérrez-Sanz
authors.forename: Cristina
authors.initials: C
authors.lastname: Tapia
authors.forename: Marta C
authors.initials: MC
authors.lastname: Marques
authors.forename: Sonia
authors.initials: S
authors.lastname: Zacarias
authors.forename: Marisela
authors.initials: M
authors.lastname: Vélez
authors.forename: Inês A C
authors.initials: IA
authors.lastname: Pereira
authors.forename: Antonio L
authors.initials: AL
authors.lastname: De Lacey
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000378:metabolism*; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism*; D006864:Hydrogenase / Q000737:chemistry / Q000378:metabolism*; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011522:Protons
chemical_list: D008051:Lipid Bilayers; D010743:Phospholipids; D011522:Protons; D006046:Gold; D006859:Hydrogen; D006864:Hydrogenase
keywords: enzymatic electrodes; hydrogen; hydrogenase; phospholipids; proton gradients