title: Ca(2+) movement and apoptosis induced by deltamethrin in Madin-Darby canine kidney canine renal tubular cells.
abstract: This study explored the effect of deltamethrin, a pesticide, on free Ca(2+) concentration [Ca(2+)]i, viability, and apoptosis in Madin-Darby canine kidney (MDCK) canine renal tubular cells. Deltamethrin at concentrations between 10μM and 40μM evoked [Ca(2+)]i rises in a concentration-dependent manner. The Ca(2+) entry was inhibited by nifedipine, econazole, phorbol 12-myristate 13-acetate, and SKF96365. Treatment with the endoplasmic reticulum Ca(2+) pump inhibitor 2,5-di-tert-butylhydroquinone (BHQ) in a Ca(2+)-free medium abolished deltamethrin-induced [Ca(2+)]i rise. Treatment with deltamethrin also abolished BHQ-induced [Ca(2+)]i rise. Inhibition of phospholipase C (PLC) activity with U73122 abolished deltamethrin-evoked [Ca(2+)]i rise. Deltamethrin killed cells at 30-60μM in a concentration-dependent manner. The cytotoxic effect of deltamethrin was not reversed by prechelating cytosolic Ca(2+) with the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid. Annexin V/propidium iodide staining data suggest that 30-50μM deltamethrin induced apoptosis. Together, in MDCK renal tubular cells, deltamethrin induced [Ca(2+)]i rises that involved Ca(2+) entry through protein kinase C-mediated store-operated Ca(2+) channels, and PLC-dependent Ca(2+) release from the endoplasmic reticulum. Deltamethrin also induced Ca(2+)-independent cell death that might involve apoptosis.
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Fang-Jin
authors.initials: FJ
authors.lastname: Liu
authors.affiliation: Department of Nursing, Division of Basic Medical Sciences, Chang Gung University of Science and Technology, Chia-Yi, Taiwan; Chronic Diseases and Health Promotion Research Center, Chang Gung University of Science and Technology, Chia-Yi, Taiwan.
authors.forename: Chiang-Ting
authors.initials: CT
authors.lastname: Chou
authors.affiliation: Department of Medicine, Kaohsiung Veterans General Hospital, Tainan, Taiwan.
authors.forename: Jin Shiung
authors.initials: JS
authors.lastname: Cheng
authors.affiliation: Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
authors.forename: Hong-Tai
authors.initials: HT
authors.lastname: Chang
authors.affiliation: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
authors.forename: Wei-Zhe
authors.initials: WZ
authors.lastname: Liang
authors.affiliation: Department of Nursing, Tzu Hui Institute of Technology, Pingtung, Taiwan.
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Kuo
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Soong-Yu
authors.initials: SY
authors.lastname: Kuo
authors.affiliation: Department of Pharmacy, Tajen University, Pingtung, Taiwan.
authors.forename: Daih-Huang
authors.initials: DH
authors.lastname: Kuo
authors.affiliation: Department of Pharmacy, Tajen University, Pingtung, Taiwan.
authors.forename: Pochuen
authors.initials: P
authors.lastname: Shieh
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.affiliation: Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. Electronic address: crjan@isca.vghks.gov.tw.
authors.forename: Chung-Ren
authors.initials: CR
authors.lastname: Jan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D007684:Kidney Tubules / Q000166:cytology* / Q000378:metabolism; D061985:Madin Darby Canine Kidney Cells; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*
chemical_list: D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D002118:Calcium
keywords: Apoptosis; Ca(2+); Deltamethrin; Madin–Darby canine kidney; Renal cells