title: Design, synthesis, and application of OB2C combinatorial peptide and peptidomimetic libraries.
abstract: The "one-bead two-compound" (OB2C) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. An OB2C library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. When live cells are incubated with such OB2C libraries, every bead will be coated with a monolayer of cells. The cell membranes of the captured cells facing the bead surface are exposed to the library compounds tethered to each bead. A specific biochemical or cellular response can be detected with an appropriate reporter system. The OB2C method enables investigators to rapidly discover synthetic molecules that not only interact with cell-surface receptors but can also stimulate or inhibit downstream cell signaling. To demonstrate this powerful method, one OB2C peptide library and two OB2C peptidomimetic libraries were synthesized and screened against Molt-4 lymphoma cells to discover "death ligands." Apoptosis of the bead-bound cells was detected with immunocytochemistry using horseradish peroxidase (HRP)-conjugated anti-cleaved caspase-3 antibody and 3,3'-diaminobenzidine as a substrate. Two novel synthetic "death ligands" against Molt-4 cells were discovered using this OB2C library approach.
authors.affiliation: Department of Biochemistry & Molecular Medicine, University of California Davis, Sacramento, CA, 95817, USA.
authors.forename: Ruiwu
authors.initials: R
authors.lastname: Liu
authors.forename: Tsung-Chieh
authors.initials: TC
authors.lastname: Shih
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Deng
authors.forename: Lara
authors.initials: L
authors.lastname: Anwar
authors.forename: Sara
authors.initials: S
authors.lastname: Ahadi
authors.forename: Pappanaicken
authors.initials: P
authors.lastname: Kumaresan
authors.forename: Kit S
authors.initials: KS
authors.lastname: Lam
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D019151:Peptide Library; D057786:Peptidomimetics / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D019151:Peptide Library; D057786:Peptidomimetics