title: Effect of aldosterone-producing adenoma on endothelial function and Rho-associated kinase activity in patients with primary aldosteronism.
abstract: The purpose of this study was to evaluate vascular function and activity of Rho-associated kinases (ROCKs) in patients with primary aldosteronism. Vascular function, including flow-mediated vasodilation (FMD) and nitroglycerine-induced vasodilation, and ROCK activity in peripheral leukocytes were evaluated in 21 patients with aldosterone-producing adenoma (APA), 23 patients with idiopathic hyperaldosteronism (IHA), and 40 age-, sex-, and blood pressure-matched patients with essential hypertension (EHT). FMD was significantly lower in the APA group than in the IHA and EHT groups (3.2±2.0% versus 4.6±2.3% and 4.4±2.2%; P<0.05, respectively), whereas there was no significant difference in FMD between the IHA and EHT groups. There was no significant difference in nitroglycerine-induced vasodilation in the 3 groups. ROCK activity was higher in the APA group than in the IHA and EHT groups (1.29±0.57 versus 1.00±0.46 and 0.81±0.36l; P<0.05, respectively), whereas there was no significant difference in ROCK activity between the IHA and EHT groups. FMD correlated with age (r=-0.31; P<0.01), plasma aldosterone concentration (r=-0.35; P<0.01), and aldosterone:renin ratio (r=-0.34; P<0.01). ROCK activity correlated with age (r=-0.24; P=0.04), plasma aldosterone concentration (r=0.33; P<0.01), and aldosterone:renin ratio (r=0.46; P<0.01). After adrenalectomy, FMD and ROCK activity were restored in patients with APA. APA was associated with both endothelial dysfunction and increased ROCK activity compared with those in IHA and EHT. APA may have a higher risk of future cardiovascular events.
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Kenji
authors.initials: K
authors.lastname: Oki
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Masato
authors.initials: M
authors.lastname: Kajikawa
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Ayumu
authors.initials: A
authors.lastname: Nakashima
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Maruhashi
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Iwamoto
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Akimichi
authors.initials: A
authors.lastname: Iwamoto
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Nozomu
authors.initials: N
authors.lastname: Oda
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hidaka
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Yasuki
authors.initials: Y
authors.lastname: Kihara
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Nobuoki
authors.initials: N
authors.lastname: Kohno
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Chayama
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Chikara
authors.initials: C
authors.lastname: Goto
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Aibara
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: Kensuke
authors.initials: K
authors.lastname: Noma
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.).
authors.forename: James K
authors.initials: JK
authors.lastname: Liao
authors.affiliation: From the Departments of Cardiovascular Medicine (T. Matsumoto, M.K., T. Maruhashi, Y.I., A.I., N.O., T.H., Y.K.) and Molecular and Internal Medicine (K.O., N.K.), Graduate School of Biomedical and Health Sciences, Department of Gastroenterology and Metabolism, Institute of Biomedical and Health Sciences (K.C.), and Department of Cardiovascular Regeneration and Medicine, Research Institute for Radiation Biology and Medicine (Y.A., K.N., Y.H.), Hiroshima University, Hiroshima, Japan; Division of Regeneration and Medicine, Medical Center for Translational and Clinical Research, Hiroshima University Hospital, Hiroshima, Japan (A.N., Y.H.); Department of Physical Therapy, Hiroshima International University, Hiroshima, Japan (C.G.); and Section of Cardiology, University of Chicago Medical Center, IL (J.K.L.). yhigashi@hiroshima-u.ac.jp.
authors.forename: Yukihito
authors.initials: Y
authors.lastname: Higashi
mesh_terms: D000236:Adenoma / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D000328:Adult; D000450:Aldosterone; D001794:Blood Pressure / Q000502:physiology*; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D006929:Hyperaldosteronism / Q000097:blood / Q000150:complications / Q000503:physiopathology*; D006973:Hypertension / Q000097:blood / Q000209:etiology / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D014664:Vasodilation / Q000502:physiology*; D054460:rho-Associated Kinases / Q000097:blood*
chemical_list: D000450:Aldosterone; D054460:rho-Associated Kinases
keywords: Rho-associated kinases; aldosterone; hyperaldosteronism; hypertension