title: Escherichia coli-induced temporal and differential secretion of heat-shock protein 70 and interleukin-1β by human fetal membranes in a two-compartment culture system.
abstract: BACKGROUND
  Escherichia coli is recognized as an etiological bacteria associated with chorioamnionitis and the preterm premature rupture of fetal membranes. This pathological condition induces pro-inflammatory cytokines and degradative metalloproteinases, which are considered biological markers secreted in an acute stage of infection. Heat-shock proteins (HSPs) are an important component of the innate immunity response and are found in different pathological conditions. They have not been previously measured in human fetal membranes in response to infectious conditions. We hypothesized that the choriodecidual tissue and amniotic epithelium secreted temporal and differential Hsp-60, Hsp-70, and interleukin (IL)-1β mediated by E. coli infection.
  
  
  METHODS
  Fetal membranes were mounted in a two-compartment culture system and infected with two passes of live E. coli at different doses (10², 10⁴, 10⁵, and 10⁶ colony-forming units (CFU)/mL) and intervals of incubation (3, 6, and 24 h). The culture medium was collected, and Hsp-60, Hsp-70, and IL-1β were assessed using the enzyme-linked immunosorbent assay (ELISA) method.
  
  
  RESULTS
  After 3 and 6 h of infection, E. coli induced an increase in Hsp-70 secretion in the choriodecidual tissue. However, after 24 h of incubation, Hsp-70 was downregulated and we observed an increase in IL-1β secretion. By contrast, E. coli induced a lower Hsp-60 secretion in the amnion compared to Hsp-70.
  
  
  CONCLUSIONS
  Human fetal membranes responded actively to E. coli infection, with an increase in Hsp-70 during the first hours of infection. After 24 h, there was an increase in the liberation of IL-1β.
authors.affiliation: Department of Obstetrics and Gynecology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Montes Urales #800, Col. Lomas de Virreyes cp, 11000 Mexico City, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Osorio-Caballero
authors.affiliation: Department of Biochemistry and Molecular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: C
authors.initials: C
authors.lastname: Perdigón-Palacio
authors.affiliation: Department of Cellular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: G
authors.initials: G
authors.lastname: García-López
authors.affiliation: Department of Biochemistry, School of Medicine, UNAM. Apdo. Postal 70-159, Copilco, Coyoacán, Mexico City, Mexico.
authors.forename: O
authors.initials: O
authors.lastname: Flores-Herrera
authors.affiliation: Department of Biochemistry, School of Medicine, UNAM. Apdo. Postal 70-159, Copilco, Coyoacán, Mexico City, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Olvera-Sánchez
authors.affiliation: Department of Infectology and Immunology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Morales-Méndez
authors.affiliation: Department of Infectology and Immunology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: I
authors.initials: I
authors.lastname: Sosa-González
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas SouthWestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Acevedo
authors.affiliation: Department of Biochemistry and Molecular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: A M
authors.initials: AM
authors.lastname: Guzmán-Grenfell
authors.affiliation: Department of Cellular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Molina-Hernández
authors.affiliation: Department of Cellular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico.
authors.forename: N F
authors.initials: NF
authors.lastname: Díaz
authors.affiliation: Department of Biochemistry and Molecular Biology, National Institute of Perinatology "Isidro Espinosa de los Reyes", Mexico City, Mexico. Electronic address: h.flores@inper.mx.
authors.forename: H
authors.initials: H
authors.lastname: Flores-Herrera
mesh_terms: D000650:Amnion / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D018834:Chaperonin 60 / Q000378:metabolism; D002821:Chorioamnionitis / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D002823:Chorion / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D003656:Decidua / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D005321:Extraembryonic Membranes / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D005260:Female; D050957:HSP110 Heat-Shock Proteins / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007113:Immunity, Innate; D053583:Interleukin-1beta / Q000378:metabolism*; D007700:Kinetics; D024101:Mitochondrial Proteins / Q000378:metabolism; D011247:Pregnancy; D046509:Tissue Culture Techniques; D015854:Up-Regulation
chemical_list: D018834:Chaperonin 60; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C121993:HSPA4 protein, human; C060293:HSPD1 protein, human; C508585:IL1B protein, human; D053583:Interleukin-1beta; D024101:Mitochondrial Proteins
keywords: Amnion; Chorioamnionitis; Choriodecidual; Escherichia coli; Heat-shock protein; Interleukin-1β