title: BEACH-domain proteins act together in a cascade to mediate vacuolar protein trafficking and disease resistance in Arabidopsis.
abstract: Membrane trafficking to the protein storage vacuole (PSV) is a specialized process in seed plants. However, this trafficking mechanism to PSV is poorly understood. Here, we show that three types of Beige and Chediak-Higashi (BEACH)-domain proteins contribute to both vacuolar protein transport and effector-triggered immunity (ETI). We screened a green fluorescent seed (GFS) library of Arabidopsis mutants with defects in vesicle trafficking and isolated two allelic mutants gfs3 and gfs12 with a defect in seed protein transport to PSV. The gene responsible for the mutant phenotype was found to encode a putative protein belonging to group D of BEACH-domain proteins, which possess kinase domains. Disruption of other BEACH-encoding loci in the gfs12 mutant showed that BEACH homologs acted in a cascading manner for PSV trafficking. The epistatic genetic interactions observed among BEACH homologs were also found in the ETI responses of the gfs12 and gfs12 bchb-1 mutants, which showed elevated avirulent bacterial growth. The GFS12 kinase domain interacted specifically with the pleckstrin homology domain of BchC1. These results suggest that a cascade of multiple BEACH-domain proteins contributes to vacuolar protein transport and plant defense.
authors.affiliation: Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Ooi-kock
authors.initials: OK
authors.lastname: Teh
authors.affiliation: Research Centre for Cooperative Projects, Hokkaido University, Kita-ku, Sapporo 060-8638, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Hatsugai
authors.affiliation: Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Tamura
authors.affiliation: Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Fuji
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Tabata
authors.affiliation: Functional Genomics Facility, National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Katsushi
authors.initials: K
authors.lastname: Yamaguchi
authors.affiliation: Functional Genomics Facility, National Institute for Basic Biology, Okazaki 444-8585, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Shingenobu
authors.affiliation: Department of Biology and IGSP Center for Systems Biology, Duke University, Durham, NC 27708, USA.
authors.forename: Masashi
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Division of Evolutionary Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan; School of Life Science, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan.
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Hasebe
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sawa
authors.affiliation: Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Shimada
authors.affiliation: Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: ihnishi@gr.bot.kyoto-u.ac.jp.
authors.forename: Ikuko
authors.initials: I
authors.lastname: Hara-Nishimura
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D060467:Disease Resistance; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014617:Vacuoles / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins
keywords: Arabidopsis thaliana; BEACH-domain protein; plant immunity; protein storage vacuoles; vacuolar protein transport