title: [Tetramethoxystilbene, a selective CYP1B1 inhibitor, suppresses adipogenesis of C3H10T1/2 pluripotent stem cells].
abstract: OBJECTIVE
  To investigate the inhibitory effects of tetramethoxystilbene, a selective CYP1B1 inhibitor, on adipogenic differentiation of C3H10T1/2 multi-potent mesenchymal cells.
  
  
  METHODS
  In vitro cultured C3H10T1/2 cells at full confluence were induced by adipogenic agents (10 µg/ml insulin, 2 µmol/L dexamethasone and 0.5 mmol/L 3-isobutyl-1-methylxanthine) and exposed simultaneously to TMS at the final concentrations of 1.0, 2.0 or 4.0 µg/ml. Oil Red-O staining was used to observe the cell differentiation. The expression of peroxisome proliferator-activated receptor gamma (PPARγ) and its target genes cluster of differentiation 36 (CD36) and fatty acid binding protein 4 (FABP4) were quantified by real-time RT-PCR and Western blotting.
  
  
  RESULTS
  Oil Red-O staining and TG contents revealed that TMS suppressed induced differentiation of C3H10T1/2 cells. TMS exposure of the cells dose-dependently decreased both mRNA and protein expressions of PPARγ, a key nuclear transcription factor during adipogenesis, and also lowered the mRNA expressions of PPARγ target genes CD36 and FABP4.
  
  
  CONCLUSIONS
  TMS can suppress adipogenic differentiation of C3H10T1/2 cells by inhibiting PPARγ
authors.affiliation: Department of Obstetrics , Renmin Hospital, Wuhan University, Wuhan 430071, China.E-mail: 359568292@qq.com.
authors.forename: Cui-Fang
authors.initials: CF
authors.lastname: Fan
authors.forename: An-Na
authors.initials: AN
authors.lastname: Zhu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Huang
authors.forename: Lu
authors.initials: L
authors.lastname: Li
authors.forename: Su-Qing
authors.initials: SQ
authors.lastname: Wang
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D065633:Cytochrome P-450 CYP1B1; D065607:Cytochrome P-450 Enzyme Inhibitors / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D008809:Mice, Inbred C3H; D047495:PPAR gamma / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D047495:PPAR gamma; D012333:RNA, Messenger; D013267:Stilbenes; C585498:Cyp1b1 protein, mouse; D065633:Cytochrome P-450 CYP1B1