title: IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans.
abstract: RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Anikó I
authors.initials: AI
authors.lastname: Nagy
authors.affiliation: Research Group in Molecular, Cellular and Genomic Biomedicine, Health Research Institute-La Fe, Valencia, Spain Centre for Biomedical Network Research on Rare Diseases (CIBERER), Valencia, Spain.
authors.forename: Rafael P
authors.initials: RP
authors.lastname: Vázquez-Manrique
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Marie
authors.initials: M
authors.lastname: Lopez
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Christo P
authors.initials: CP
authors.lastname: Christov
authors.affiliation: Research Group in Molecular, Cellular and Genomic Biomedicine, Health Research Institute-La Fe, Valencia, Spain Centre for Biomedical Network Research on Rare Diseases (CIBERER), Valencia, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Sequedo
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Mareike
authors.initials: M
authors.lastname: Herzog
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Anna E
authors.initials: AE
authors.lastname: Herlihy
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Maxime
authors.initials: M
authors.lastname: Bodak
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK.
authors.forename: Roxani
authors.initials: R
authors.lastname: Gatsi
authors.affiliation: Department of Zoology, University of Cambridge, Cambridge, UK hab28@cam.ac.uk.
authors.forename: Howard A
authors.initials: HA
authors.lastname: Baylis
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008954:Models, Biological; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*
chemical_list: D012330:RNA, Double-Stranded; D015544:Inositol 1,4,5-Trisphosphate
keywords: C. elegans; RNA interference; calcium signalling; enhanced RNAi; inositol 1,4,5‐trisphosphate