title: Identification and function of adenosine A3 receptor in afferent arterioles.
abstract: Adenosine plays an important role in regulation of renal microcirculation. All receptors of adenosine, A1, A2A, A2B, and A3, have been found in the kidney. However, little is known about the location and function of the A3 receptor in the kidney. The present study determined the expression and role of A3 receptors in mediating the afferent arteriole (Af-Art) response and studied the interaction of A3 receptors with angiotensin II (ANG II), A1 and A2 receptors on the Af-Art. We found that the A3 receptor expressed in microdissected isolated Af-Art and the mRNA levels of A3 receptor were 59% of A1. In the isolated microperfused Af-Art, A3 receptor agonist IB-MECA did not have a constrictive effect. Activation of A3 receptor dilated the preconstricted Af-Art by norepinephrine and blunted the vasoconstrictive effect of both adenosine A1 receptor activation and ANG II on the Af-Art, respectively. Selective A2 receptor antagonist (both A2A and A2B) had no effect on A3 receptor agonist-induced vasodilation, indicating that the dilatory effect of A3 receptor activation is not mediated by activation of A2 receptor. We conclude that the A3 receptor is expressed in the Af-Art, and activation of the A3 receptor dilates the Af-Art.
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida; Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi; ylu2@umc.edu.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Pharmacology, University of Mississippi Medical Center, Jackson, Mississippi; and.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Carlstrom
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Shaohui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Yiling
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Jin
authors.initials: J
authors.lastname: Wei
authors.affiliation: Department of Pharmacology, University of Mississippi Medical Center, Jackson, Mississippi; and.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Roman
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
authors.forename: Xichun
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida; Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi;
authors.forename: Ruisheng
authors.initials: R
authors.lastname: Liu
mesh_terms: D058909:Adenosine A3 Receptor Agonists / Q000494:pharmacology; D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001160:Arterioles / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000098:blood supply*; D008297:Male; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000378:metabolism; D020239:Receptor Cross-Talk; D043682:Receptor, Adenosine A1 / Q000378:metabolism; D043684:Receptor, Adenosine A3 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D043683:Receptors, Adenosine A2 / Q000378:metabolism; D012079:Renal Circulation / Q000187:drug effects; D015398:Signal Transduction; D014664:Vasodilation / Q000187:drug effects
chemical_list: D058909:Adenosine A3 Receptor Agonists; D012333:RNA, Messenger; D043682:Receptor, Adenosine A1; D043684:Receptor, Adenosine A3; D043683:Receptors, Adenosine A2; D000804:Angiotensin II
keywords: A3 receptor; Af-Art; adenosine; tubuloglomerular feedback