title: Incidence of viral infection detected by PCR and real-time PCR in childhood community-acquired pneumonia: a meta-analysis.
abstract: Several studies examining the incidence of viral infection in childhood community-acquired pneumonia (CAP) utilizing polymerase chain reaction (PCR) or real-time PCR methods have been reported. We systematically searched Pubmed and Embase for studies reporting the incidence of respiratory viral infection in childhood CAP. The pooled incidences of viral infection were calculated with a random-effects model. Sources of heterogeneity were explored by subgroup analysis and a univariant metaregression analysis. We included 21 eligible reports in our study. We found significant heterogeneity on the incidence of viral infection in childhood CAP. The random effects pooled incidence was 57.4% (95% confidence interval (CI): 50.8-64.1). The pooled incidence of mixed infection was 29.3% (95%CI: 23.0-35.6) with considerable heterogeneity. The pooled incidence of mixed infection was 29.3% (95%CI: 23.0-35.6). Rhinovirus, respiratory syncytial virus (RSV) and bocavirus were found to be the three most common viruses in childhood CAP. We also demonstrated that respiratory viruses were detected in 76.1% of patients aged ≤ 1 year, 63.1% of patients aged 2-5 years and 27.9% of patients aged ≥ 6 years. We conclude that respiratory viruses are widely detected in paediatric patients with CAP by PCR or real-time PCR methods. More than half of viral infections are probably concurrent with bacterial infections. Rhinovirus, RSV and bocavirus are the three most frequent viruses identified in childhood CAP; the incidence of viral infection decreased with age.
authors.affiliation: Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Cai
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Wu
authors.forename: Ting
authors.initials: T
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.forename: Yi
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D060085:Coinfection; D017714:Community-Acquired Infections / Q000175:diagnosis / Q000453:epidemiology; D005260:Female; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D015994:Incidence; D008297:Male; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000453:epidemiology; D010850:Picornaviridae Infections / Q000175:diagnosis / Q000453:epidemiology; D011024:Pneumonia, Viral / Q000175:diagnosis / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000453:epidemiology; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012229:Rhinovirus / Q000302:isolation & purification*
keywords: child; community-acquired pneumonia; incidence; meta-analysis; respiratory virus