title: Identification of secretaglobin Scgb2a1 as a target for developmental reprogramming by BPA in the rat prostate.
abstract: Secretoglobins are a superfamily of secreted proteins thought to participate in inflammation, tissue repair, and tumorigenesis. Secretoglobin family 2A member 1 (Scgb2a1) is a component of prostatein, a major androgen-binding protein secreted by the rat prostate. Using a rat model for developmental reprogramming of susceptibility to prostate carcinogenesis, we identified, by RNA-seq, that Scgb2a1 is significantly upregulated (>100-fold) in the prostate of adult rats neonatally exposed to bisphenol A (BPA), with increased gene expression confirmed by quantitative RT-PCR and chromatin immunoprecipitation for histone H3 lysine 9 acetylation. Bisulfite analysis of both CpG islands located within 10 kb of the Scgb2a1 promoter identified significant hypomethylation of the CpG island upstream of the transcription start site of this gene in the reprogrammed prostate. These data suggest that expression of Scgb2a1 in the adult prostate could be epigenetically reprogrammed by BPA exposure during prostate development, with potential implications for cancer risk and response to chemotherapeutics associated with prostatein binding.
authors.affiliation: a Center for Translational Cancer Research; Institute of Biosciences and Technology ; The Texas A&M University System Health Science Center ; Houston , TX USA.
authors.forename: Rebecca Lee Yean
authors.initials: RL
authors.lastname: Wong
authors.forename: Quan
authors.initials: Q
authors.lastname: Wang
authors.forename: Lindsey S
authors.initials: LS
authors.lastname: Treviño
authors.forename: Maarten C
authors.initials: MC
authors.lastname: Bosland
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Mario
authors.initials: M
authors.lastname: Medvedovic
authors.forename: Gail S
authors.initials: GS
authors.lastname: Prins
authors.forename: Kurunthachalam
authors.initials: K
authors.lastname: Kannan
authors.forename: Shuk-Mei
authors.initials: SM
authors.lastname: Ho
authors.forename: Cheryl Lyn
authors.initials: CL
authors.lastname: Walker
mesh_terms: D000107:Acetylation; D000818:Animals; D000831:Animals, Newborn; D001559:Benzhydryl Compounds / Q000633:toxicity*; D065150:Cellular Reprogramming / Q000187:drug effects*; D018899:CpG Islands / Q000187:drug effects; D019175:DNA Methylation / Q000187:drug effects; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D006657:Histones / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008297:Male; D060788:Mammaglobin B / Q000378:metabolism*; D010636:Phenols / Q000633:toxicity*; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011467:Prostate / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000139:chemically induced; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004968:Estrogens, Non-Steroidal; D006657:Histones; D008239:Lysine; D060788:Mammaglobin B; D010636:Phenols; C556468:Scgb2a1 protein, rat; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: BPA; BPA, Bisphenol A; ChIP, chromatin immunoprecipitation; DNA methylation; E, Estrogen; EDCs, Endocrine Disrupting Compounds; PIN, Prostatic Intraepithelial Neoplasia; RNA-seq, RNA-sequencing.; Scgb2a1; T, Testosterone; developmental reprogramming; prostate cancer; prostatein; qRT-PCR, quantitative real-time PCR