title: Patterned, but not tonic, optogenetic stimulation in motor thalamus improves reaching in acute drug-induced Parkinsonian rats.
abstract: High-frequency deep brain stimulation (DBS) in motor thalamus (Mthal) ameliorates tremor but not akinesia in Parkinson's disease. The aim of this study was to investigate whether there are effective methods of Mthal stimulation to treat akinesia. Glutamatergic Mthal neurons, transduced with channelrhodopsin-2 by injection of lentiviral vector (Lenti.CaMKII.hChR2(H134R).mCherry), were selectively stimulated with blue light (473 nm) via a chronically implanted fiber-optic probe. Rats performed a reach-to-grasp task in either acute drug-induced parkinsonian akinesia (0.03-0.07 mg/kg haloperidol, s.c.) or control (vehicle injection) conditions, and the number of reaches was recorded for 5 min before, during, and after stimulation. We compared the effect of DBS using complex physiological patterns previously recorded in the Mthal of a control rat during reaching or exploring behavior, with tonic DBS delivering the same number of stimuli per second (rate-control 6.2 or 1.8 Hz, respectively) and with stimulation patterns commonly used in other brain regions to treat neurological conditions (tonic 130 Hz, theta burst (TBS), and tonic 15 Hz rate-control for TBS). Control rats typically executed >150 reaches per 5 min, which was unaffected by any of the stimulation patterns. Acute parkinsonian rats executed <20 reaches, displaying marked akinesia, which was significantly improved by stimulating with the physiological reaching pattern or TBS (both p < 0.05), whereas the exploring and all tonic patterns failed to improve reaching. Data indicate that the Mthal may be an effective site to treat akinesia, but the pattern of stimulation is critical for improving reaching in parkinsonian rats.
authors.affiliation: Departments of Physiology.
authors.forename: Sonja
authors.initials: S
authors.lastname: Seeger-Armbruster
authors.affiliation: Anatomy, and.
authors.forename: Clémentine
authors.initials: C
authors.lastname: Bosch-Bouju
authors.affiliation: Anatomy, and.
authors.forename: Shane T C
authors.initials: ST
authors.lastname: Little
authors.affiliation: Departments of Physiology.
authors.forename: Roseanna A
authors.initials: RA
authors.lastname: Smither
authors.affiliation: Biochemistry, Otago School of Medical Science, Brain Health Research Centre, University of Otago, Dunedin 9054, New Zealand.
authors.forename: Stephanie M
authors.initials: SM
authors.lastname: Hughes
authors.affiliation: Departments of Physiology.
authors.forename: Brian I
authors.initials: BI
authors.lastname: Hyland
authors.affiliation: Anatomy, and louise.parr-brownlie@otago.ac.nz.
authors.forename: Louise C
authors.initials: LC
authors.lastname: Parr-Brownlie
mesh_terms: D000818:Animals; D046690:Deep Brain Stimulation / Q000379:methods*; D008297:Male; D009043:Motor Activity / Q000502:physiology*; D062308:Optogenetics / Q000379:methods*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000503:physiopathology* / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D013788:Thalamus / Q000503:physiopathology*
keywords: Parkinson's disease; motor thalamus; optogenetic stimulation; patterns of stimulation; reaching task; theta burst stimulation