title: Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) rapidly identified a critical missense mutation (P236T) of bovine ACADVL gene affecting growth traits.
abstract: Acyl-CoA dehydrogenase, very long chain (ACADVL), encoding ACADVL protein, targets the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway and plays an important role in body metabolism and oxidation of long chain fatty acid releasing energy. Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) is an easy-to-operate, rapid, inexpensive, and exact method for SNP genotyping. Herein, T-ARMS-PCR was carried out to detect a critical missense mutation (AC_000176:g.2885C>A; Pro236Thr) within the ACADVL gene in 644 individuals from two cattle breeds. In order to evaluate the accuracy of the T-ARMS-PCR at this locus, the genotype of the sampled individuals was also identified by PCR-RFLP. The concordance between these two methods was 98.76%. Statistical analysis showed that the bovine ACADVL gene had a significant effect on chest width (P<0.05), chest depth (P<0.05), and hip width (P<0.05) in the Qinchuan breed. The cattle with AA genotype had superior growth traits compared to cattle with AC and/or CC genotypes. The "A" allele had positive effects on growth traits. Therefore, T-ARMS-PCR can replace PCR-RFLP for rapid genotyping of this mutation, which could be used as a DNA marker for selecting individuals with superior growth traits in the Qinchuan breed. These findings contribute to breeding and genetics in beef cattle industry.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: sihuanzhang1990@163.com.
authors.forename: Sihuan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Dang
authors.affiliation: College of Life Sciences, Heze University, Heze, Shangdong 274015, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Heze University, Heze, Shangdong 274015, China.
authors.forename: Qiaomei
authors.initials: Q
authors.lastname: Qin
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China. Electronic address: lanxianyong79@nwsuaf.edu.cn.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
mesh_terms: D042964:Acyl-CoA Dehydrogenase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D060005:Genotyping Techniques; D020125:Mutation, Missense; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: D042964:Acyl-CoA Dehydrogenase
keywords: Acyl-CoA dehydrogenase, very long chain (ACADVL); Cattle; Growth traits; Single nucleotide polymorphism (SNP); Tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR)