title: Pseudomonas aeruginosa activates the quorum sensing LuxR response regulator through secretion of 2-aminoacetophenone.
abstract: In this study we identify a volatile compound produced by Pseudomonas aeruginosa, which can specifically activate the LuxR quorum-sensing response regulator of Vibrio fischeri. Comparative gas-chromatography analysis between P. aeruginosa wild type and a ΔlasR mutant strain implied that the active volatile is 2-aminoacetophenone. The use of synthetic 2-aminoacetophenone and in silico docking analyses verified the activity of the molecule and provided putative interacting residues within the binding site.
authors.affiliation: Department of Plant Pathology and Microbiology, The Robert H Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel. yael.helman@mail.huji.ac.il.
authors.forename: I
authors.initials: I
authors.lastname: Kviatkovski
authors.forename: L
authors.initials: L
authors.lastname: Chernin
authors.forename: T
authors.initials: T
authors.lastname: Yarnitzky
authors.forename: I
authors.initials: I
authors.lastname: Frumin
authors.forename: N
authors.initials: N
authors.lastname: Sobel
authors.forename: Y
authors.initials: Y
authors.lastname: Helman
mesh_terms: D000098:Acetophenones / Q000378:metabolism*; D048908:Aliivibrio fischeri / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015374:Biosensing Techniques; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D053038:Quorum Sensing; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D000098:Acetophenones; D001426:Bacterial Proteins; D012097:Repressor Proteins; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; C055495:2-aminoacetophenone