title: New Export Pathway in Plasmodium falciparum-Infected Erythrocytes: Role of the Parasite Group II Chaperonin, PfTRiC.
abstract: The export of numerous proteins to the plasma membrane of its host erythrocyte is essential for the virulence and survival of the malaria parasite Plasmodium falciparum. The Maurer's clefts, membrane structures transposed by the parasite in the cytoplasm of its host erythrocyte, play the role of a marshal platform for such exported parasite proteins. We identify here the export pathway of three resident proteins of the Maurer's clefts membrane: the proteins are exported as soluble forms in the red cell cytoplasm to the Maurer's clefts membrane in association with the parasite group II chaperonin (PfTRIC), a chaperone complex known to bind and address a large spectrum of unfolded proteins to their final location. We have also located the domain of interaction with PfTRiC within the amino-terminal domain of one of these Maurer's cleft proteins, PfSBP1. Because several Maurer's cleft membrane proteins with different export motifs seem to follow the same route, we propose a general role for PfTRiC in the trafficking of malarial parasite proteins to the host erythrocyte.
authors.affiliation: University Montpellier II, CNRS UMR 5235, University Montpellier I, Dynamique des Interactions Membranaires Normales et Pathologiques, 34095, Montpellier Cedex 5, France.
authors.forename: Alassane
authors.initials: A
authors.lastname: Mbengue
authors.forename: Emilie
authors.initials: E
authors.lastname: Vialla
authors.forename: Laurence
authors.initials: L
authors.lastname: Berry
authors.forename: Gamou
authors.initials: G
authors.lastname: Fall
authors.forename: Nicolas
authors.initials: N
authors.lastname: Audiger
authors.forename: Edith
authors.initials: E
authors.lastname: Demettre-Verceil
authors.forename: David
authors.initials: D
authors.lastname: Boteller
authors.forename: Catherine
authors.initials: C
authors.lastname: Braun-Breton
mesh_terms: D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism / Q000469:parasitology*; D056385:Group II Chaperonins / Q000235:genetics / Q000378:metabolism*; D006790:Host-Parasite Interactions / Q000502:physiology*; D006801:Humans; D052940:Merozoites / Q000378:metabolism / Q000469:parasitology; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D021381:Protein Transport; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D056385:Group II Chaperonins
keywords: Plasmodium falciparum; TRiC group II chaperonin; erythrocyte; malaria; protein export