title: The effects of different intraabdominal pressure protocols in laparoscopic procedures on oxidative stress markers and morphology in rat ovaries.
abstract: BACKGROUND
  To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model.
  
  
  OBJECTIVE
  The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model.
  
  
  METHODS
  Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Pp15, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated.
  
  
  RESULTS
  In Groups Pp10 and Pp15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Pp10 and IPp15.
  
  
  CONCLUSIONS
  Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than low-pressure pneumoperitoneum methods.
authors.affiliation: Fatsa Gynecology and Maternity Training and Research Hospital of TC Ministry of Health, Ordu, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Biler
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Sait
authors.initials: S
authors.lastname: Yucebilgin
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Sendag
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Akman
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Akdemir
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Utku
authors.initials: U
authors.lastname: Ates
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Yigit
authors.initials: Y
authors.lastname: Uyanikgil
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Yilmaz-Dilsiz
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Ege University, Bornova, İzmir, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Sezer
mesh_terms: D034841:Abdominal Cavity; D000818:Animals; D015415:Biomarkers / Q000097:blood; D002245:Carbon Dioxide / Q000008:administration & dosage; D005260:Female; D007327:Insufflation; D019194:Ischemic Preconditioning / Q000009:adverse effects / Q000379:methods; D010535:Laparoscopy / Q000009:adverse effects; D008315:Malondialdehyde / Q000097:blood; D010053:Ovary / Q000378:metabolism*; D018384:Oxidative Stress; D011028:Pneumoperitoneum, Artificial; D011312:Pressure; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D013997:Time Factors
chemical_list: D015415:Biomarkers; D002245:Carbon Dioxide; D008315:Malondialdehyde