title: Development and characterizations of a miniature capillary electrophoresis mass spectrometry system.
abstract: A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.
authors.affiliation: Department of Biomedical Engineering, Beijing Institute of Technology , Beijing 100081, China.
authors.forename: Muyi
authors.initials: M
authors.lastname: He
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Xue
authors.forename: Yinna
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zejian
authors.initials: Z
authors.lastname: Huang
authors.forename: Xiang
authors.initials: X
authors.lastname: Fang
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Ouyang
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D001274:Atmospheric Pressure; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D013058:Mass Spectrometry / Q000295:instrumentation*; D010455:Peptides / Q000032:analysis*
chemical_list: D010455:Peptides