title: Characterization of a virulent dog-originated rabies virus affecting more than twenty fallow deer (Dama dama) in Inner Mongolia, China.
abstract: Rabies has emerged as a serious problem in the most recent years in northern China. A rabies virus (RABV) isolate, IMDRV-13, was recovered from brain samples of dog-bitten rabid fallow deer (Dama dama) in a farm in Hohhot, Inner Mongolia. We tested the susceptibility of mouse neuroblastoma (MNA) cells and BSR cells as well as that of adult mice to IMDRV-13. The isolate was found to be a virulent isolate with an equivalent pathogenicity index (0.12) and a slight lower neurotropism index (1.07) compared with those of challenge virus standard, CVS-24, which was 0.13 and 1.23, respectively. The complete genome of IMDRV-13 was determined subsequently and found to be 11,924 nucleotides (nt) in length with the same genomic organization as other RABVs. Phylogenetic tree based on complete genome sequences of 43 RABV isolates and strains indicated that IMDRV-13, along with other two isolates in Inner Mongolia, CNM1101C and CNM1104D, clustered within the dog-associated China I clade, which is also the dominant lineage in the current rabies epidemic in China. In addition, sequence analysis of the glycoprotein G identified an amino acid substitution (I338→T338) unique to the IMDRV-13 within antigenic sites III (330-338), this mutation also leads to an additional potential N-glycosylation site (N336), which may represent a useful model to study relationship of N-glycosylation in G protein and specific properties such as pathogenicity or host adaption of RABV.
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhu
authors.affiliation: China Institute of Veterinary Drugs Control, Beijing, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Xiqun
authors.initials: X
authors.lastname: Shao
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Hengxing
authors.initials: H
authors.lastname: Ba
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Fengxue
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
authors.forename: Hualei
authors.initials: H
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Na
authors.initials: N
authors.lastname: Sun
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China.
authors.forename: Jingqiang
authors.initials: J
authors.lastname: Ren
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China. Electronic address: tcscsp@126.com.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
authors.affiliation: State Key Laboratory for Molecular Biology of Special Economic Animal Sciences, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, China. Electronic address: wenyongjun@caas.cn.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Wen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D003670:Deer / Q000821:virology*; D004285:Dogs; D016679:Genome, Viral; D006031:Glycosylation; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D011818:Rabies / Q000662:veterinary*; D011820:Rabies virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014774:Virulence
chemical_list: D014764:Viral Proteins
keywords: Fallow deer; Genomic analysis; N-Glycosylation sites; Pathogenicity; Phylogeny; Rabies virus