title: Single-nucleotide polymorphism identification and genotyping inCamelina sativa.
abstract: Camelina sativa, a largely relict crop, has recently returned to interest due to its potential as an industrial oilseed. Molecular markers are key tools that will allowC. sativato benefit from modern breeding approaches. Two complementary methodologies, capture of 3' cDNA tags and genomic reduced-representation libraries, both of which exploited second generation sequencing platforms, were used to develop a low density (768) Illumina GoldenGate single nucleotide polymorphism (SNP) array. The array allowed 533 SNP loci to be genetically mapped in a recombinant inbred population ofC. sativa. Alignment of the SNP loci to theC. sativagenome identified the underlying sequenced regions that would delimit potential candidate genes in any mapping project. In addition, the SNP array was used to assess genetic variation among a collection of 175 accessions ofC. sativa, identifying two sub-populations, yet low overall gene diversity. The SNP loci will provide useful tools for future crop improvement ofC. sativa.
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada ; School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu, 180 009 JK India.
authors.forename: Ravinder
authors.initials: R
authors.lastname: Singh
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Venkatesh
authors.initials: V
authors.lastname: Bollina
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Erin E
authors.initials: EE
authors.lastname: Higgins
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Wayne E
authors.initials: WE
authors.lastname: Clarke
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Christina
authors.initials: C
authors.lastname: Eynck
authors.affiliation: National Research Council Canada, 110 Gymnasium Place, Saskatoon, S7N 0W9 Canada.
authors.forename: Christine
authors.initials: C
authors.lastname: Sidebottom
authors.affiliation: Plant Gene Resources Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Richard
authors.initials: R
authors.lastname: Gugel
authors.affiliation: Department of Plant Breeding, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
authors.forename: Rod
authors.initials: R
authors.lastname: Snowdon
authors.affiliation: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, S7N 0X2 Canada.
authors.forename: Isobel A P
authors.initials: IA
authors.lastname: Parkin
keywords: Camelina sativa; Diversity; Genetic mapping; Polyploidy; Reduced representation; SNP