title: Purification, crystallization and preliminary X-ray crystallographic studies of Rv3899c from Mycobacterium tuberculosis.
abstract: Rv3899c, a hypothetical protein from Mycobacterium tuberculosis that is conserved within the mycobacteria, is predicted to be secreted and has been found in culture filtrates. Here, Rv3899c has been cloned, expressed in Escherichia coli and purified using standard chromatographic techniques. The hanging-drop vapour-diffusion method with PEG 3350 as a precipitant was used to crystallize the protein. N-terminal sequencing results showed that the amino-acid sequence of the crystallized protein began with GATAG, indicating that it is a fragment containing residues 184-410 of Rv3899c. Rv3899c184-410 crystals exhibited the symmetry of space group P2(1)2(1)2(1), with unit-cell parameters a=49.88, b=54.72, c=75.52 Å, α=β=γ=90°, and diffracted to a resolution of 1.90 Å.
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors.forename: Yingjia
authors.initials: Y
authors.lastname: Song
authors.affiliation: The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Laboratory of Biomacromolecules and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: De-Feng
authors.initials: DF
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors.forename: Honglin
authors.initials: H
authors.lastname: Li
authors.affiliation: The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology and the School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
authors.affiliation: National Laboratory of Biomacromolecules and Laboratory of Noncoding RNA, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
authors.forename: Da-Cheng
authors.initials: DC
authors.lastname: Wang
authors.affiliation: The Fourth People's Hospital Foshan, Foshan 528000, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Bi
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D009169:Mycobacterium tuberculosis
chemical_list: D001426:Bacterial Proteins
keywords: Mycobacterium tuberculosis; Rv3899c