title: A convection-driven long-range linear gradient generator with dynamic control.
abstract: We developed a novel gradient generator to achieve long range and linear chemical gradients with a dynamic control function. The length of the gradient can be on the centimetre scale. The gradient profile can be tuned by changing the flow rates. The device can work in both high flow rate regimes with large shear stress and low flow rate regimes with minimum shear stress. The drug screening function was demonstrated by the viability test of PC-9 cancer cells.
authors.affiliation: Department of Electrical and Computer Engineering and Singapore Institute for Neurotechnology (SINAPSE), National University of Singapore, Singapore 117576. elelc@nus.edu.sg.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Chia-Hung
authors.initials: CH
authors.lastname: Chen
authors.forename: Zhuolin
authors.initials: Z
authors.lastname: Xiang
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.forename: Chengkuo
authors.initials: C
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival; D018481:Convection; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*