title: A polyprotein-expressing salmonid alphavirus replicon induces modest protection in atlantic salmon (Salmo salar) against infectious pancreatic necrosis.
abstract: Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection.
authors.affiliation: Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, 0033 Oslo, Norway. azila.binti.abdullah@nmbu.no.
authors.forename: Azila
authors.initials: A
authors.lastname: Abdullah
authors.affiliation: Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, 0033 Oslo, Norway. christel.moraeus.olsen@nmbu.no.
authors.forename: Christel M
authors.initials: CM
authors.lastname: Olsen
authors.affiliation: MSD Animal Health Norway, Thormøhlensgate 55, N-5008 Bergen, Norway. kjartan.hodneland@merck.com.
authors.forename: Kjartan
authors.initials: K
authors.lastname: Hodneland
authors.affiliation: Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, P.O. Box 8146 Dep, 0033 Oslo, Norway. espen.rimstad@nmbu.no.
authors.forename: Espen
authors.initials: E
authors.lastname: Rimstad
mesh_terms: D000524:Alphavirus / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D004337:Drug Carriers; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D018061:Infectious pancreatic necrosis virus / Q000235:genetics / Q000276:immunology*; D007273:Injections, Intramuscular; D020815:Polyproteins / Q000235:genetics / Q000276:immunology*; D020317:Salmo salar; D016019:Survival Analysis; D016896:Treatment Outcome; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D004337:Drug Carriers; D020815:Polyproteins; D014614:Vaccines, Synthetic; D014764:Viral Proteins; D014765:Viral Vaccines