title: Novel exons and splice variants in the human antibody heavy chain identified by single cell and single molecule sequencing.
abstract: Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
authors.affiliation: Dept. of Bioengineering, Stanford University, Stanford, California, United States of America.
authors.forename: Christopher
authors.initials: C
authors.lastname: Vollmers
authors.affiliation: Dept. of Bioengineering, Stanford University, Stanford, California, United States of America.
authors.forename: Lolita
authors.initials: L
authors.lastname: Penland
authors.affiliation: Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America.
authors.forename: Jad N
authors.initials: JN
authors.lastname: Kanbar
authors.affiliation: Dept. of Bioengineering, Stanford University, Stanford, California, United States of America; Dept. of Applied Physics, Stanford University, Stanford, California, United States of America; Howard Hughes Medical Institute, Chevy Chase, Maryland, United States of America.
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Quake
mesh_terms: D001402:B-Lymphocytes; D005091:Exons; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D012326:RNA Splicing; D017423:Sequence Analysis, RNA
chemical_list: D007143:Immunoglobulin Heavy Chains