title: GhCFE1A, a dynamic linker between the ER network and actin cytoskeleton, plays an important role in cotton fibre cell initiation and elongation.
abstract: Fibre cell initiation and elongation is critical for cotton fibre development. However, little is known about the regulation of initiation and elongation during fibre cell development. Here, the regulatory role of a novel protein GhCFE1A was uncovered. GhCFE1A is preferentially expressed at initiation and rapid elongation stages during fibre development; in addition, much higher expression of GhCFE1A was detected at the fibre initiation stage in ﬁbreless cotton mutants than in the fibre-bearing TM-1 wild-type. Importantly, overexpression of GhCFE1A in cotton not only delayed fibre cell elongation but also significantly reduced the density of lint and fuzz fibre initials and stem trichomes. Yeast two-hybrid assay showed that GhCFE1A interacted with several actin proteins, and the interaction was further confirmed by co-sedimentation assay. Interestingly, a subcellular localization assay showed that GhCFE1A resided on the cortical endoplasmic reticulum (ER) network and co-localized with actin cables. Moreover, the density of F-actin filaments was shown to be reduced in GhCFE1A-overexpressing fibres at the rapid elongation stage compared with the wild-type control. Taken together, the results demonstrate that GhCFE1A probably functions as a dynamic linker between the actin cytoskeleton and the ER network, and plays an important role in fibre cell initiation and elongation during cotton fibre development.
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fenni
authors.initials: F
authors.lastname: Lv
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Haihai
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Libo
authors.initials: L
authors.lastname: Han
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yinping
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhidi
authors.initials: Z
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhaosheng
authors.initials: Z
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics & Germplasm Enhancement, Hybrid Cotton R & D Engineering Research Center, MOE, Nanjing Agricultural University, Nanjing 210095, China moelab@njau.edu.cn.
authors.forename: Wangzhen
authors.initials: W
authors.lastname: Guo
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism*; D000199:Actins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D032561:Cotton Fiber; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018547:Plant Stems / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D017422:Sequence Analysis, DNA; D064201:Trichomes / Q000235:genetics / Q000254:growth & development
chemical_list: D000199:Actins; D010940:Plant Proteins
keywords: Actin cytoskeleton; GhCFE1A.; cotton fibre; endoplasmic reticulum; fibre elongation; fibre initiation