title: In situ synthesis of self-assembled three-dimensional graphene-magnetic palladium nanohybrids with dual-enzyme activity through one-pot strategy and its application in glucose probe.
abstract: The self-assembled three-dimensional graphene nanohybrids with in situ-formed Fe3O4 and Pd nanoparticles on it (3DRGO_Fe3O4-Pd) are first synthesized by the one-pot solvothermal method, which have intrinsic peroxidase-like and oxidase-like activity. The catalytic mechanism is analyzed by the electron spin resonance (ESR), fluorescence, and electrochemical methods. The mimic enzyme catalytic activity of 3DRGO_Fe3O4-Pd is much higher than those of monometallic loaded nanohybrids and their physical mixture, probably caused by synergistic effect between Pd and Fe3O4 nanoparticles. The 3DRGO_Fe3O4-Pd nanohybrids was reproducible, stable, and reusable. After 10 cycles, the catalytic activity was still higher than 90%, and the morphology and structure were basically unchanged. Based on its high peroxidase-like activity, especially the enhanced affinity toward H2O2, a new colorimetric detection method for reduced glutathione (GSH) and glucose has been designed using H2O2 as an intermediary, which provides a simple, sensitive, and selective way to detect urine glucose of diabetes with a wide linear range and low detection limit.
authors.affiliation: National Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Zheng
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.forename: Huiqing
authors.initials: H
authors.lastname: Song
authors.forename: Xinrui
authors.initials: X
authors.lastname: Zhao
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Hongli
authors.initials: H
authors.lastname: Chen
authors.forename: Xingguo
authors.initials: X
authors.lastname: Chen
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002384:Catalysis; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry*; D010165:Palladium / Q000737:chemistry*; D009195:Peroxidase / Q000737:chemistry*
chemical_list: D010165:Palladium; D006108:Graphite; D009195:Peroxidase; D005947:Glucose; D052203:Ferrosoferric Oxide
keywords: colorimetry; dual-enzyme activity; glucose; one-pot; three-dimensional graphene nanohybrids