title: [CpG-ODN activation of TLR9 signaling pathways and NF-κB in STZ-induced diabetic rats].
abstract: OBJECTIVE
  To observe the mRNA and protein expression of TLR9, NF-κB and serum level of Th1/Th2 cytokines when stimulated with different concentration of CpG-ODN in STZ-induced diabetic rats.
  
  
  METHODS
  40 SD male rats were randomly divided into four groups:normal control group (Group I), type 1 diabetes control group (Group II), low-dose CpG-ODN treated type 1 diabetes group (Group III), high dose CpG-ODN treated type 1 diabetes group (Group IV). 60 µg /kg and 120 µg /kg CpG-ODN were injected to rats of Group III and IV when inducing type 1 diabetes model.mRNA and protein levels of TLR9 and NF-κB in spleen and pancreas were detected by fluorescence quantitative PCR and Western blotting.Serum TNF-α, IL-6 and IFN-γ were measured by ELISA.
  
  
  RESULTS
  TLR9 protein expression levels were much higher in spleen than in the pancreas among these four groups. In spleen, TLR9 and NF-κB mRNA and protein level were significantly higher in Group III and IV than those in Group I and II, and the expression level was higher in Group IV than in Group III. In pancreas, TLR9 and NF-κB protein level were significantly higher in Group III and IV than those in Group I and II, and the expression level was also higher in Group IV than Group III.Serum TNF-α and IFN-γ level in Group III and IV significantly higher than in Group I and II, and serum levels of TNF-α and IFN-γ in Group IV was higher than in Group III.
  
  
  CONCLUSIONS
  CpG-ODN activation of TLR9-MyD88 signaling pathways results in dose-effect NF-κB stimulation in STZ-induced diabetic rats.
authors.affiliation: Department of Endocrinology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou 325000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jinjue
authors.initials: J
authors.lastname: Zheng
authors.forename: Youjin
authors.initials: Y
authors.lastname: Pan
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Email: huyunliang66@163.com.
authors.forename: Yunliang
authors.initials: Y
authors.lastname: Hu
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D015850:Interleukin-6; D008297:Male; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D013154:Spleen; D013311:Streptozocin; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha
chemical_list: C408982:CPG-oligonucleotide; D015850:Interleukin-6; D016328:NF-kappa B; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; C489640:Tlr9 protein, rat; D051217:Toll-Like Receptor 9; D014409:Tumor Necrosis Factor-alpha; D013311:Streptozocin