title: Clinical and tumor significance of tropomyosin-1 expression levels in renal cell carcinoma.
abstract: Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults and has been described as one of the deadliest of cancers affecting the genitourinary tract. Tropomyosin is a two-stranded α-helical coiled coil protein found in cell cytoskeletons. One of its isoforms, tropomyosin-1 (TPM1) has been reported as a novel tumor-suppressor gene and is downregulated in many solid tumors. However the expression level and function of TPM1 in RCC have not yet been determined. In the present study, we evaluated the TPM1-4 mRNA and TPM1 protein levels in RCC tissue samples. TPM1-overexpressing OSRC-2 and 786-O cell lines were also used to investigate the impact of TPM1 on RCC cells. We found that TPM1 was significantly and specifically downregulated in the RCC tissues. TPM1 expression was associated with tumor size, smoking status, Fuhrman grade and the prognosis of RCC patients. After TPM1 transfection, the migratory and invasive abilities of the OSRC-2 and 786-O cell lines were both reduced when compared to the control groups. Meanwhile, apoptosis was also enhanced in these two RCC cell lines following TPM1 transfection. Taken together, TPM1 exhibits characteristics of a tumor-suppressor gene while being overexpressed in RCC cell lines.
authors.affiliation: Department of Urology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Guan
authors.affiliation: Department of Urology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Epigenetics Laboratory, School of Life Science, Jilin University, Changchun, Jilin 130012, P.R. China.
authors.forename: Jingji
authors.initials: J
authors.lastname: Jin
authors.affiliation: Epigenetics Laboratory, School of Life Science, Jilin University, Changchun, Jilin 130012, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Urology, The First Bethune Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
authors.forename: Chunxi
authors.initials: C
authors.lastname: Wang
authors.affiliation: Epigenetics Laboratory, School of Life Science, Jilin University, Changchun, Jilin 130012, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection / Q000379:methods; D014335:Tropomyosin / Q000096:biosynthesis / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; C490775:TPM1 protein, human; C518659:TPM2 protein, human; C498783:TPM3 protein, human; C501213:TPM4 protein, human; D014335:Tropomyosin; D025521:Tumor Suppressor Proteins