title: Bovine Papillomavirus Type 13 Expression in the Urothelial Bladder Tumours of Cattle.
abstract: Bovine papillomavirus type 13 (BPV-13), a novel Deltapapillomavirus, has been found associated with urothelial tumours of the urinary bladder of cattle grazing on lands infested with bracken fern. BPV-13 was detected in 28 of 39 urothelial tumours. Diagnosis was based on sequencing of L1 and E5 amplicons from tumour samples. The nucleotide sequences generated from these amplicons showed a 100% homology with the sequences of BPV-13 L1 and E5 DNA found in Brazil from a fibropapilloma of the ear in a cow and from equine sarcoids in two horses. GenBank accession number of our representative BPV-13 sequences is JQ798171.1. Furthermore, mRNA encoding BPV-13 E5 oncoprotein was also documented, and its expression was also shown by immunohistochemistry and immunofluorescence in the basal and suprabasal urothelial tumour cells. In twenty-three tumours, BPV-13 was simultaneously found with BPV-2, a Deltapapillomavirus genus, species 4. The latter virus was detected by amplifying and sequencing a 154-bp-sized DNA fragment of BPV-2 E5. In addition, BPV-13 by itself was seen to be expressed in five BPV-2-negative urothelial tumours. This study shows that BPV-13 is present in urothelial tumour cells thus sharing biological properties with BPV-1 and BPV-2. Although further studies are needed, BPV-13 appears to be another worldwide infectious agent responsible for a distressing disease causing severe economic losses in cattle industry.
authors.affiliation: Settore Malattie Infettive, Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy. sante.roperto@unina.it.
authors.forename: S
authors.initials: S
authors.lastname: Roperto
authors.affiliation: Settore Patologia Generale, Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Russo
authors.affiliation: Dipartimento di Medicina Veterinaria, Università di Perugia, Perugia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Leonardi
authors.affiliation: Settore Patologia Generale, Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Martano
authors.affiliation: Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (NA), Italy.
authors.forename: F
authors.initials: F
authors.lastname: Corrado
authors.affiliation: Settore Patologia Generale, Dipartimento di Medicina Veterinaria e Produzioni Animali, Università di Napoli Federico II, Napoli, Italy.
authors.forename: M G
authors.initials: MG
authors.lastname: Riccardi
authors.affiliation: Dipartimento di Biologia, Università di Napoli Federico II, Napoli, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Roperto
mesh_terms: D000818:Animals; D001483:Base Sequence; D052688:Bovine papillomavirus 1 / Q000235:genetics / Q000302:isolation & purification*; D001938:Brazil; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D001743:Urinary Bladder / Q000821:virology; D001749:Urinary Bladder Neoplasms / Q000662:veterinary* / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Bovine papillomavirus type 13; Deltapapillomavirus; cattle; urinary bladder; urothelial tumours