title: A study on the selection of chemiluminescence system for the flow injection determination of the total polyphenol index of plant-derived foods.
abstract: Different chemiluminescence systems based on luminol, permanganate, manganese(IV) and cerium(IV) reagents were compared regarding their sensitivity and selectivity to determine plant polyphenols. Among the seventeen systems tested, Mn(IV)-formaldehyde-hexametaphosphate was considered to be the most suitable for polyphenols detection. The developed flow injection method (FI-CL) based on enhancing effect of polyphenols on Mn(IV) chemiluminescence is characterised by low detection limit of gallic acid (0.02μgL(-1)) and high precision (RSD=1.7%). The calibration graph was linear from 0.1 to 100μgL(-1). The selectivity studies revealed that the FI-CL method ensures accurate determination of the total polyphenols content in food samples. The method was successfully applied to analysis of a variety of plant-derived foods (wine, tea, cereal coffee, fruit and vegetable juices, herbs and spices). The proposed method is superior to conventional spectrophotometric assays due to its higher sample throughput (195samplesh(-1)), simplicity, sensitivity and, above all, higher selectivity.
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Nalewajko-Sieliwoniuk
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland. Electronic address: j.malejko@uwb.edu.pl.
authors.forename: Julita
authors.initials: J
authors.lastname: Malejko
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Święczkowska
authors.affiliation: Department of Analytical Chemistry, Institute of Chemistry, University of Białystok, Hurtowa 1, 15-399 Białystok, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Kowalewska
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D008345:Manganese / Q000032:analysis; D010945:Plants, Edible / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D008345:Manganese; D008165:Luminol
keywords: Cerium(IV); Chemiluminescence; Flow injection analysis; Food; Luminol; Manganese(IV); Polyphenolic antioxidants; Potassium permanganate