title: Multidimensional electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) for quantitative analysis of the proteome and phosphoproteome in clinical and biomedical research.
abstract: Quantitative proteomics and phosphoproteomics have become key disciplines in understanding cellular processes. Fundamental research can be done using cell culture providing researchers with virtually infinite sample amounts. In contrast, clinical, pre-clinical and biomedical research is often restricted to minute sample amounts and requires an efficient analysis with only micrograms of protein. To address this issue, we generated a highly sensitive workflow for combined LC-MS-based quantitative proteomics and phosphoproteomics by refining an ERLIC-based 2D phosphoproteomics workflow into an ERLIC-based 3D workflow covering the global proteome as well. The resulting 3D strategy was successfully used for an in-depth quantitative analysis of both, the proteome and the phosphoproteome of murine cytomegalovirus-infected mouse fibroblasts, a model system for host cell manipulation by a virus. In a 2-plex SILAC experiment with 150 μg of a tryptic digest per condition, the 3D strategy enabled the quantification of ~75% more proteins and even ~134% more peptides compared to the 2D strategy. Additionally, we could quantify ~50% more phosphoproteins by non-phosphorylated peptides, concurrently yielding insights into changes on the levels of protein expression and phosphorylation. Beside its sensitivity, our novel three-dimensional ERLIC-strategy has the potential for semi-automated sample processing rendering it a suitable future perspective for clinical, pre-clinical and biomedical research.
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Otto-Hahn-Straße 6b, 44227 Dortmund, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Loroch
authors.affiliation: Heinrich-Pette-Institut-Leibniz-Institut für Experimentelle Virologie, Martinistraße 52, 20251 Hamburg, Germany.
authors.forename: Tim
authors.initials: T
authors.lastname: Schommartz
authors.affiliation: Heinrich-Pette-Institut-Leibniz-Institut für Experimentelle Virologie, Martinistraße 52, 20251 Hamburg, Germany.
authors.forename: Wolfram
authors.initials: W
authors.lastname: Brune
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Otto-Hahn-Straße 6b, 44227 Dortmund, Germany.
authors.forename: René Peiman
authors.initials: RP
authors.lastname: Zahedi
authors.affiliation: Leibniz-Institut für Analytische Wissenschaften-ISAS-e.V., Otto-Hahn-Straße 6b, 44227 Dortmund, Germany; University of Aberdeen, School of Natural & Computing Sciences, Meston Building, Meston Walk, Old Aberdeen, AB24 3UE, Germany; Medizinische Fakultät, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany. Electronic address: albert.sickmann@isas.de.
authors.forename: Albert
authors.initials: A
authors.lastname: Sickmann
mesh_terms: D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D057927:Hydrophobic and Hydrophilic Interactions; D051379:Mice; D041681:NIH 3T3 Cells; D010750:Phosphoproteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D055672:Static Electricity; D053719:Tandem Mass Spectrometry / Q000379:methods; D057188:Workflow
chemical_list: D010750:Phosphoproteins; D020543:Proteome
keywords: Clinical research; ERLIC; Multidimensional separation; Phosphoproteomics; Proteomics