title: A methyltransferase required for proper timing of the vernalization response in Arabidopsis.
abstract: Prolonged exposure to winter cold enables flowering in many plant species through a process called vernalization. In Arabidopsis, vernalization results from the epigenetic silencing of the floral repressor flowering locus C (FLC) via a Polycomb Repressive Complex 2 (PRC2)-mediated increase in the density of the epigenetic silencing mark H3K27me3 at FLC chromatin. During cold exposure, a gene encoding a unique, cold-specific PRC2 component, vernalization insensitive 3 (VIN3), which is necessary for PRC2-mediated silencing of FLC, is induced. Here we show that set domain group 7 (SDG7) is required for proper timing of VIN3 induction and of the vernalization process. Loss of SDG7 results in a vernalization-hypersensitive phenotype, as well as more rapid cold-mediated up-regulation of VIN3. In the absence of cold, loss of SDG7 results in elevated levels of long noncoding RNAs, which are thought to participate in epigenetic repression of FLC. Furthermore, loss of SDG7 results in increased H3K27me3 deposition on FLC chromatin in the absence of cold exposure and enhanced H3K27me3 spreading during cold treatment. Thus, SDG7 is a negative regulator of vernalization, and loss of SDG7 creates a partially vernalized state without cold exposure.
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706; and.
authors.forename: Joohyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706; and.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Yun
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg 67084, France.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, Strasbourg 67084, France.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Shen
authors.affiliation: Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706; and amasino@biochem.wisc.edu.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Amasino
mesh_terms: D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant; D008780:Methyltransferases / Q000378:metabolism*; D009154:Mutation
chemical_list: D008780:Methyltransferases
keywords: SET DOMAIN GROUP 7; flowering time; vernalization