title: AC5 Surgical Hemostat™ as an effective hemostatic agent in an anticoagulated rat liver punch biopsy model.
abstract: Intra-operative and postoperative bleeding is a major concern in surgical procedures for patients taking anticoagulant medications, or where anticoagulants are used to prevent potential life-threatening embolic complications. Heparin is the anticoagulant used most frequently and has an immediate effect on blood clotting, lasting 4 to 6h. Although synthetic self-assembling peptides have been shown to achieve rapid hemostasis in small animals, none have adequately addressed the potential for hemostasis in the presence of anticoagulant therapy in-vivo. Our goal was to investigate the hemostatic activity of a known synthetic self-assembling peptide in animals treated and untreated with heparin anticoagulation therapy. Using a rat liver puncture model, animals were treated with known synthetic peptide AC5 Surgical Hemostatic Device™, or saline controls. Time-to-hemostasis and coagulation times were recorded in both heparinized and non-heparinized animals. Here we show that AC5™ was able to achieve rapid hemostasis equivalently in both heparinized and non-heparinized animals.
  
  
  BACKGROUND
  Intra-operative and postoperative bleeding is a major concern in surgical procedures for patients taking anticoagulant medications. In this work the effective hemostasis was demonstrated both in heparinized and non-heparinized animals using self-assembling peptides.
authors.affiliation: Department of Surgical Research and Techniques, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
authors.forename: Domokos
authors.initials: D
authors.lastname: Csukas
authors.affiliation: Nanomedicine Research and Education Center, Semmelweis University, Budapest, Hungary; SeroScience Ltd., Budapest, Hungary.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Urbanics
authors.affiliation: Department of Psychology, University of Mannheim, Mannheim, Germany.
authors.forename: Annie
authors.initials: A
authors.lastname: Moritz
authors.affiliation: Department of Ophthalmology, Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany; Department of Brain & Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: rutledge@medma.uni-heidelberg.de.
authors.forename: Rutledge
authors.initials: R
authors.lastname: Ellis-Behnke
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000627:therapeutic use*; D001706:Biopsy; D001777:Blood Coagulation / Q000187:drug effects*; D016063:Blood Loss, Surgical / Q000517:prevention & control; D004195:Disease Models, Animal; D006470:Hemorrhage / Q000188:drug therapy*; D006487:Hemostasis / Q000187:drug effects*; D006490:Hemostatics / Q000627:therapeutic use*; D006493:Heparin / Q000627:therapeutic use*; D008099:Liver / Q000293:injuries; D008297:Male; D010455:Peptides / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000925:Anticoagulants; D006490:Hemostatics; D010455:Peptides; D006493:Heparin
keywords: AC5™; Anticoagulant; Hemostasis; Heparin; Rat hepatic bleeding model