title: Carbon encapsulated iron oxide nanoparticles surface engineered with polyethylene glycol-folic acid to induce selective hyperthermia in folate over expressed cancer cells.
abstract: Carbon encapsulated iron oxide nanoparticles (CEIO-NPs) prepared by carbon arc method were successfully applied for in vitro magnetic hyperthermia. The CEIO-NPs were chemically oxidized and surface modified with PEG-FA for selective tumor localization in cancer cells that over expresses the folate receptors (FR(+)). The size, morphology, heating efficiency, biocompatibility and in vitro cell uptake of CEIO-PEG-FA NPs are extensively characterized. The as-prepared nanoparticles have generated quick heating (43-45°C) upon exposure to an alternating magnetic field (AMF) with the saturation magnetization of 25emu/g. The LDH cytotoxic assay demonstrated that the nanoparticle did not affect the viability of normal human fibroblast. The quantitative and cellular uptake studies by TEM confirmed the selective and increased uptake of CEIO-PEG-FA NPs when compared to the CEIO-nanoparticles. In conclusion, CEIO-PEG-FA NPs have the potential to induce magnetic hyperthermia in FR(+) cells via the receptor mediated endocytosis uptake mechanism.
authors.affiliation: Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Taiwan.
authors.forename: S
authors.initials: S
authors.lastname: Sadhasivam
authors.affiliation: Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chennai, India.
authors.forename: S
authors.initials: S
authors.lastname: Savitha
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Chun-Jen
authors.initials: CJ
authors.lastname: Wu
authors.affiliation: Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Taiwan; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: double@ntu.edu.tw.
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
authors.affiliation: Institute of Physical Chemistry, Polish Academy of Sciences Warsaw, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Stobiński
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002460:Cell Line; D004705:Endocytosis / Q000502:physiology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005492:Folic Acid / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D060526:Magnetic Fields; D058185:Magnetite Nanoparticles; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols; D002244:Carbon; D005492:Folic Acid
keywords: Carbon-arc; Folic acid; Hela cells; Hyperthermia; Iron oxide nanoparticles