title: Simultaneous determination of insulin and its analogues in pharmaceutical formulations by micellar electrokinetic chromatography.
abstract: A simple and efficient MEKC method was developed to simultaneously determine human insulin, its five analogues, the main degradation products and the excipients usually present in injection formulations. A very fast method with a total analysis time of 3 min was then successfully validated for the analysis of human insulin and the quality control of commercial formulations was carried out.
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lamalle
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Servais
authors.affiliation: Department of Diabetes, Nutrition and Metabolic disorders, CHU Sart Tilman, University of Liege, Belgium.
authors.forename: Régis P
authors.initials: RP
authors.lastname: Radermecker
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium.
authors.forename: Jacques
authors.initials: J
authors.lastname: Crommen
authors.affiliation: Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmacy, CIRM, University of Liege, CHU, B36, B-4000 Liege, Belgium. Electronic address: Marianne.fillet@ulg.ac.be.
authors.forename: Marianne
authors.initials: M
authors.lastname: Fillet
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods; D006801:Humans; D007328:Insulin / Q000737:chemistry*; D011786:Quality Control
chemical_list: D007328:Insulin
keywords: Counterfeit medicines; Insulin; Micellar electrokinetic chromatography; Quality control; Stability