title: Determination of mutagenicity of the precipitate formed by sodium hypochlorite and chlorhexidine using the Ames test.
abstract: The aim of this study was to determine the direct mutagenic potential of any precipitate formed by combining sodium hypochlorite (NaOCl) and chlorhexidine (CHX). The precipitates formed by NaOCl and CHX were dissolved in 100% dimethyl sulfoxide and cultured with mutant Salmonella Typhimurium strains. The cells were observed for reverse mutation. The numbers of positive/mutated wells were statistically compared with those in the background plates using the two-sample proportion independent t-test. The precipitates were not found to be significantly more mutagenic than the background plates. Within the limitations of this study, the results suggest that the precipitates formed when sodium hypochlorite and chlorhexidine contact did not show mutagenic (and are therefore carcinogenic) potential.
authors.affiliation: Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, USA.
authors.forename: Pranali
authors.initials: P
authors.lastname: Patil
authors.affiliation: Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, USA.
authors.forename: Anita
authors.initials: A
authors.lastname: Aminoshariae
authors.affiliation: Department of Biostatistics, Case Western Reserve University, Cleveland, Ohio, USA.
authors.forename: Jarrod
authors.initials: J
authors.lastname: Harding
authors.affiliation: Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Montagnese
authors.affiliation: Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, USA.
authors.forename: Andre
authors.initials: A
authors.lastname: Mickel
mesh_terms: D002710:Chlorhexidine / Q000633:toxicity*; D009153:Mutagens; D012388:Root Canal Irrigants / Q000633:toxicity*; D012486:Salmonella typhimurium; D012973:Sodium Hypochlorite / Q000633:toxicity*
chemical_list: D009153:Mutagens; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; D002710:Chlorhexidine
keywords: Carcinogenicity; chlorhexidine; endodontic; irrigants; mutagenicity; root canal