title: New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal pH metry in extraesophageal gastroesophageal reflux disease.
abstract: OBJECTIVE
  To determine the laryngeal H+K+-ATPase and pharyngeal pH in patients with laryngopharyngeal reflux (LPR)-symptoms as well as to assess the symptom scores during PPI therapy.
  
  
  METHODS
  Endoscopy was performed to exclude neoplasia and to collect biopsies from the posterior cricoid area (immunohistochemistry and PCR analysis). Immunohistochemical staining was performed with monoclonal mouse antibodies against human H+K+-ATPase. Quantitative real-time RT-PCR for each of the H+K+-ATPase subunits was performed. The pH values were assessed in the aerosolized environment of the oropharynx (DxpH Catheter) and compared to a subsequently applied combined pH/MII measurement.
  
  
  RESULTS
  Twenty patients with LPR symptoms were included. In only one patient, the laryngeal H+K+-ATPase was verified by immunohistochemical staining. In another patient, real-time RT-PCR for each H+K+-ATPase subunit was positive. Fourteen out of twenty patients had pathological results in DxpH, and 6/20 patients had pathological results in pH/MII. Four patients had pathological results in both functional tests. Nine out of twenty patients responded to PPIs.
  
  
  CONCLUSIONS
  The laryngeal H+K+-ATPase can only be sporadically detected in patients with LPR symptoms and is unlikely to cause the LPR symptoms. Alternative hypotheses for the pathomechanism are needed. The role of pharyngeal pH-metry remains unclear and its use can only be recommended for patients in a research study setting.
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Valentin
authors.initials: V
authors.lastname: Becker
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Romina
authors.initials: R
authors.lastname: Drabner
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Graf
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Schlag
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Simon
authors.initials: S
authors.lastname: Nennstiel
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Buchberger
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Roland M
authors.initials: RM
authors.lastname: Schmid
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Saur
authors.affiliation: Valentin Becker, Romina Drabner, Simone Graf, Christoph Schlag, Simon Nennstiel, Roland M Schmid, Dieter Saur, Monther Bajbouj, II. Medizinische Klinik, Klinikum rechts der Isar, Technische Universität München, 81675 München, Germany.
authors.forename: Monther
authors.initials: M
authors.lastname: Bajbouj
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D005260:Female; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007150:Immunohistochemistry; D057045:Laryngopharyngeal Reflux / Q000175:diagnosis* / Q000188:drug therapy / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D007828:Laryngoscopy; D007830:Larynx / Q000187:drug effects / Q000201:enzymology* / Q000503:physiopathology; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D010614:Pharynx / Q000187:drug effects / Q000378:metabolism* / Q000503:physiopathology; D011446:Prospective Studies; D054328:Proton Pump Inhibitors / Q000627:therapeutic use; D060888:Real-Time Polymerase Chain Reaction; D016896:Treatment Outcome
chemical_list: D054328:Proton Pump Inhibitors; D017506:H(+)-K(+)-Exchanging ATPase
keywords: Gastroesophageal reflux disease; Laryngopharyngeal reflux; Pathomechanism; Proton pump inhibitor