title: Anodic stripping voltammetry of gold nanoparticles at boron-doped diamond electrodes and its application in immunochromatographic strip tests.
abstract: Anodic stripping voltammetry (ASV) of colloidal gold-nanoparticles (AuNPs) was investigated at boron-doped diamond (BDD) electrodes in 50 mM HClO4. A deposition time of 300 s at-0.2 V (vs. Ag/AgCl) was fixed as the condition for the ASV. The voltammograms showed oxidation peaks that could be attributed to the oxidation of gold. These oxidation peaks were then investigated for potential application in immunochromatographic strip tests for the selective and quantitative detection of melamine, in which AuNPs were used as the label for the antibody of melamine. Linear regression of the oxidation peak currents appeared in the concentration range from 0.05-0.6 μg/mL melamine standard, with an estimated LOD of 0.069 μg/mL and an average relative standard deviation of 8.0%. This indicated that the method could be considered as an alternative method for selective and quantitative immunochromatographic applications. The validity was examined by the measurements of melamine injected into milk samples, which showed good recovery percentages during the measurements.
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI; Department of Chemistry, Keio University 3-14-1 Hiyoshi, Yokohama 223-8522, Japan.
authors.forename: Tribidasari A
authors.initials: TA
authors.lastname: Ivandini
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI.
authors.forename: Wiyogo P
authors.initials: WP
authors.lastname: Wicaksono
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI.
authors.forename: Endang
authors.initials: E
authors.lastname: Saepudin
authors.affiliation: Department of Chemistry, Faculty of Mathematics and Science, University of Indonesia, Kampus UI.
authors.forename: Bakhadir
authors.initials: B
authors.lastname: Rismetov
authors.affiliation: Department of Chemistry, Keio University 3-14-1 Hiyoshi, Yokohama 223-8522, Japan; JST-CREST, 3-14-1 Hiyoshi, Yokohama 223-8522, Japan. Electronic address: einaga@chem.keio.ac.jp.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Einaga
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D001895:Boron / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D018130:Diamond / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D014227:Triazines / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D014227:Triazines; D006046:Gold; D018130:Diamond; C011907:melamine; D001895:Boron
keywords: Anodic stripping voltammetry; Boron-doped diamond electrode; Gold nanoparticles; Immunochromatographic strip tests; Melamine