title: Triazophos-induced oxidative stress and histomorphological changes in ovary of female Wistar rats.
abstract: Triazophos (TZ), a non-systemic broad spectrum organophosphate (OP), is being extensively used against a wide range of pests in agricultural practices. The present study was carried out to investigate the toxic effects of triazophos (TZ) in female Wistar rats. Three sub-chronic dose levels of TZ corresponding to 1/10th, 1/20th and 1/40th of LD50 were given for 30 days to adult female Wistar rats through oral intubation. During the treatment period estrous cycle was significantly altered. Activity levels of different oxidative stress (OS) parameters viz. catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were differentially altered in the ovary of treated rats. Estradiol levels were significantly high while progesterone levels were significantly reduced in plasma of 1/10th and 1/20th of LD50 TZ-treated rats. Histomorphological studies of ovary revealed increased follicular atresia and increased ovarian surface epithelial height in 1/10th and 1/20th of LD50 TZ-treated rats. Enhanced apoptosis and necrosis were also observed in ovarian granulosa cells at dose-dependent manner. Results infer that TZ exposure may lead to the number of pathophysiological conditions in female rats and severity increases at high doses.
authors.affiliation: Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India.
authors.forename: Dharmender
authors.initials: D
authors.lastname: Sharma
authors.affiliation: Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India. Electronic address: sanghagk@hotmail.com.
authors.forename: Gurinder Kaur
authors.initials: GK
authors.lastname: Sangha
authors.affiliation: Department of Zoology, Punjab Agricultural University, Ludhiana 141004, India.
authors.forename: Kuldeep Singh
authors.initials: KS
authors.lastname: Khera
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D004958:Estradiol / Q000097:blood; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D063086:Organothiophosphates / Q000633:toxicity*; D010053:Ovary / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D011374:Progesterone / Q000097:blood; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*
chemical_list: D007306:Insecticides; D063086:Organothiophosphates; D014230:Triazoles; D011374:Progesterone; D004958:Estradiol; C012584:triazophos; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005982:Glutathione Transferase
keywords: Antioxidant enzymes; Apoptosis; Ovary; Oxidative stress; Reactive oxygen species; Triazophos