title: Controlled assembly of artificial protein-protein complexes via DNA duplex formation.
abstract: DNA-protein conjugates have found a wide range of applications. This study demonstrates the formation of defined, non-native protein-protein complexes via the site specific labeling of two proteins of interest with complementary strands of single-stranded DNA in vitro. This study demonstrates that the affinity of two DNA-protein conjugates for one another may be tuned by the use of variable lengths of DNA allowing reversible control of complex formation.
authors.affiliation: †School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom.
authors.forename: Eliza
authors.initials: E
authors.lastname: Płoskoń
authors.affiliation: †School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom.
authors.forename: Sara C
authors.initials: SC
authors.lastname: Wagner
authors.affiliation: ‡Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, United States.
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Ellington
authors.affiliation: §Chemical and Biological Engineering, Chemistry of Life Processes Institute, Northwestern University Evanston, Illinois 60208, United States.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Jewett
authors.affiliation: ∥Department of Chemistry, University of Warwick, Coventry, West Midlands CV4 7AL, United Kingdom.
authors.forename: Rachel
authors.initials: R
authors.lastname: O'Reilly
authors.affiliation: †School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, United Kingdom.|⊥Department of Chemistry, King's College London, 7 Trinity Street, London SE1 1DB, United Kingdom.
authors.forename: Paula J
authors.initials: PJ
authors.lastname: Booth
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism*; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism*; D054730:Protein Interaction Domains and Motifs / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D008164:Luminescent Proteins; C527701:enhanced cyan fluorescent protein; C054562:yellow fluorescent protein, Bacteria; D049452:Green Fluorescent Proteins; D004247:DNA