title: No detectable association of IGF2BP2 and SLC30A8 genes with type 2 diabetes in the population of Hyderabad, India.
abstract: Genome-wide association studies identified novel genes associated with T2DM which have been replicated in different populations. We try to examine here if certain frequently replicated SNPs of Insulin growth factor 2 m-RNA binding protein 2 (IGF2BP2) (rs4402960, rs1470579) and Solute Carrier family 30 member 8 (SLC30A8) (rs13266634) genes, known to be implicated in insulin pathway, are associated with T2DM in the population of Hyderabad, which is considered to be a diabetic capital of India. Genotyping of the 1379 samples, 758 cases and 621 controls, for the SNPs was performed on sequenom massarray platform. The logistic regression analysis was done using SPSS software and the post-hoc power of the study was estimated using G power. The allele and genotype frequencies were similar between cases and controls, both for SNPs of IGF2BP2 and SLC30A8 genes. Logistic regression did not reveal significant allelic or genotypic association of any of the three SNPs with T2DM. Despite large sample size and adequate power, we could not replicate the association of IGF2BP2 and SLC30A8 SNPs with T2DM in our sample from Hyderabad (A.P.), India, albeit another study based on much larger sample but from heterogeneous populations from the northern parts of India showed significant association of two of the above 3 SNPs, suggesting variable nature of susceptibility of these genes in different ethnic groups. Although the IGF2BP2 and SLC30A8 genes are important in the functional pathway of Insulin secretion, it appears that these genes do not play a significant role in the susceptibility to T2DM in this population.
authors.affiliation: Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.
authors.forename: Uma Jyothi
authors.initials: UJ
authors.lastname: Kommoju
authors.affiliation: Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.
authors.forename: Jayaraj
authors.initials: J
authors.lastname: Maruda
authors.affiliation: Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.
authors.forename: Subburaj
authors.initials: S
authors.lastname: Kadarkarai
authors.affiliation: Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.
authors.forename: Kumuda
authors.initials: K
authors.lastname: Irgam
authors.affiliation: JP Endocrine Center, Ashok Nagar, Hyderabad, India.
authors.forename: Jaya Prasad
authors.initials: JP
authors.lastname: Kotla
authors.affiliation: Department of Human Genetics, Andhra University, Visakhapatnam, India.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Velaga
authors.affiliation: Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.
authors.forename: Battini
authors.initials: B
authors.lastname: Mohan Reddy
keywords: ADA, American Diabetes Association; Association; BMI, Body Mass Index; C.I., Confidence Interval.; DBP, Diastolic Blood Pressure; FPG, Fasting Plasma Glucose; GWAS, Genome wide association studies; IGF2BP2, Insulin growth factor 2 m-RNA binding protein 2; IMP1, Insulin-like growth factor 2 mRNA-binding protein 1; India; LD, Linkage Disequilibrium; O.R., Odds Ratio; PCOS, Polycystic Ovarian Syndrome; PPG, Post-prandial Plasma Glucose; Population of Hyderabad; PyPop, Python for Population Genomics; RBG, Random Plasma Glucose; SBP, Systolic Blood Pressure; SLC30A8, Solute Carrier family 30 member 8; SNP, Single Nucleotide Polymorphism; SPSS, Statistical Package for Social Sciences; Single nucleotide polymorphism (SNP); T2DM, Type 2 diabetes mellitus; Type 2 diabetes mellitus (T2DM); WHR, Waist Hip Ratio; ZnT8, Zinc Transporter 8