title: Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation.
abstract: Enzymatic catalytic processes possess great potential in chemical manufacturing, including pharmaceuticals, fuel production and food processing. However, the engineering of enzymes is severely hampered due to their low operational stability and difficulty of reuse. Here, we develop a series of stable metal-organic frameworks with rationally designed ultra-large mesoporous cages as single-molecule traps (SMTs) for enzyme encapsulation. With a high concentration of mesoporous cages as SMTs, PCN-333(Al) encapsulates three enzymes with record-high loadings and recyclability. Immobilized enzymes that most likely undergo single-enzyme encapsulation (SEE) show smaller Km than free enzymes while maintaining comparable catalytic efficiency. Under harsh conditions, the enzyme in SEE exhibits better performance than free enzyme, showing the effectiveness of SEE in preventing enzyme aggregation or denaturation. With extraordinarily large pore size and excellent chemical stability, PCN-333 may be of interest not only for enzyme encapsulation, but also for entrapment of other nanoscaled functional moieties.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Dawei
authors.initials: D
authors.lastname: Feng
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Tian-Fu
authors.initials: TF
authors.lastname: Liu
authors.affiliation: Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Jie
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Bosch
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Zhangwen
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Wei
authors.initials: W
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fujian, Fuzhou 350002, P. R. China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: Yuan
authors.affiliation: 1] Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA [2] Department of Materials Science and Engineering, Texas A&M University, College Station, Texas 77842, USA.
authors.forename: Ying-Pin
authors.initials: YP
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Xizhen
authors.initials: X
authors.lastname: Lian
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Zhi-Yuan
authors.initials: ZY
authors.lastname: Gu
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA.
authors.forename: Jihye
authors.initials: J
authors.lastname: Park
authors.affiliation: Berzelii Centre EXSELENT on Porous Materials and Inorganic and Structural Chemistry, Department of Materials and Environmental Chemistry, Stockholm University, 106 91 Stockholm, Sweden.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zou
authors.affiliation: 1] Department of Chemistry, Texas A&M University, College Station, Texas 77843-3255, USA [2] Department of Materials Science and Engineering, Texas A&M University, College Station, Texas 77842, USA.
authors.forename: Hong-Cai
authors.initials: HC
authors.lastname: Zhou
mesh_terms: D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods
chemical_list: D004800:Enzymes, Immobilized