title: High-throughput small-molecule screening in Caenorhabditis elegans.
abstract: Chemical compounds, which modulate enzymatic activities or those which induce specific phenotypes of interest, are valuable probes to study biological phenomena, as they allow modulation of enzymatic activities and temporal control of protein action. Here, we describe the methodology to conduct large-scale screens for chemical compounds that induce a desired phenotype in the roundworm Caenorhabditis elegans (C. elegans) using 96- or 384-well microtiter plates.
authors.affiliation: Department of Chemical Physiology, The Scripps Research Institute, 10550 N Torrey Pines Rd, Mail drop: MEM 268, La Jolla, CA, 92037, USA.
authors.forename: Sunitha
authors.initials: S
authors.lastname: Rangaraju
authors.forename: Gregory M
authors.initials: GM
authors.lastname: Solis
authors.forename: Michael
authors.initials: M
authors.lastname: Petrascheck
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057166:High-Throughput Screening Assays; D054852:Small Molecule Libraries
chemical_list: D054852:Small Molecule Libraries