title: Dysbiosis of fungal microbiota in the intestinal mucosa of patients with colorectal adenomas.
abstract: The fungal microbiota is an important component of the human gut microbiome and may be linked to gastrointestinal disease. In this study, the fungal microbiota of biopsy samples from adenomas and adjacent tissues was characterized by deep sequencing. Ascomycota, Glomeromycota and Basidiomycota were identified as the dominant phyla in both adenomas and adjacent tissues from all subjects. Among the 60 genera identified, the opportunist pathogens Phoma and Candida represented an average of 45% of the fungal microbiota. When analyzed at the operational taxonomic unit (OTU) level, however, a decreased diversity in adenomas was observed, and three OTUs differed significantly from the adjacent tissues. Principal Component Analysis (PCA) revealed that the core OTUs formed separate clusters for advanced and non-advanced adenomas for which the abundance of four OTUs differed significantly. Moreover, the size of adenomas and the disease stage were closely related to changes in the fungal microbiota in subjects with adenomas. This study characterized the fungal microbiota profile of subjects with adenomas and identified potential diagnostic biomarkers closely related to different stages of adenomas.
authors.affiliation: 1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Chunguang
authors.initials: C
authors.lastname: Luan
authors.affiliation: Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Xie
authors.affiliation: 1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China [2] University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Chinese National Human Genome Center, Beijing, 100176, China; SinoGenoMax Co. Ltd, Beijing. 100176, China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Miao
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
authors.forename: Na
authors.initials: N
authors.lastname: Lv
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Xiao
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China.
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Na
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Yuanmin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: 1] CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; Beijing Key Laboratory of Microbial Drug Resistance and Resistome, Beijing 100101, China [2] Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000236:Adenoma / Q000382:microbiology* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000382:microbiology* / Q000473:pathology; D064806:Dysbiosis / Q000382:microbiology*; D005260:Female; D005658:Fungi / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D007422:Intestines / Q000382:microbiology* / Q000473:pathology; D008297:Male; D064307:Microbiota; D008875:Middle Aged