title: Antiangiogenic-Like Properties of Fermented Extracts of Ayurvedic Medicinal Plants.
abstract: The three ayurvedic medicinal plants, Withania somnifera, Emblica officinalis, and Bacopa monnieri, were extracted by high-pressure static extraction using the Zippertex(®) technology. The extracts were mixed to reach quantifiable amounts of active compounds identified by high-pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis. The mixture of extracts was incubated with resting cells of the fungus Beauveria bassiana ATCC 7159. The fermentation promoted the fluidization of the starting dense mixture, while HPLC monitoring evidenced the disappearance of glucogallin from E. officinalis extract and the concomitant increase in gallic acid content. Topical exposure of the chick embryo chorioallantoic membrane (CAM) to the nonfermented extract led to the extensive necrosis and destruction of the treated membrane. However, the fermented extract was shown to be free of any toxicity. Furthermore, compared with the untreated CAM, the fermented sample reduced CAM vascularization, suggesting its antiangiogenic potency. The innocuity of the fermented extract was demonstrated using the in vivo LD50 test, the morphological examination of internal organs of treated rats, as well as the evaluation of blood biomarkers of liver damage (aspartate aminotransferase and alanine aminotransferase). The fermented extract was developed as a nutraceutical antiangiogenic treatment of age-related macular degeneration and commercialized in an oral form named Ethnodyne-Visio™.
authors.affiliation: 1 Ethnodyne, Paris, France .
authors.forename: Chérif
authors.initials: C
authors.lastname: Rabhi
authors.affiliation: 2 Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Gif-sur-Yvette, France .
authors.forename: Guillaume
authors.initials: G
authors.lastname: Arcile
authors.affiliation: 1 Ethnodyne, Paris, France .
authors.forename: Léon
authors.initials: L
authors.lastname: Cariel
authors.affiliation: 2 Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Gif-sur-Yvette, France .
authors.forename: Christine
authors.initials: C
authors.lastname: Lenoir
authors.affiliation: 2 Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Gif-sur-Yvette, France .
authors.forename: Jérome
authors.initials: J
authors.lastname: Bignon
authors.affiliation: 2 Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Gif-sur-Yvette, France .
authors.forename: Joanna
authors.initials: J
authors.lastname: Wdzieczak-Bakala
authors.affiliation: 2 Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles ICSN, Centre National de la Recherche Scientifique CNRS, Gif-sur-Yvette, France .
authors.forename: Jamal
authors.initials: J
authors.lastname: Ouazzani
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D034681:Bacopa; D052982:Beauveria / Q000378:metabolism; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000187:drug effects; D019587:Dietary Supplements; D005260:Female; D005285:Fermentation; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008268:Macular Degeneration / Q000188:drug therapy / Q000473:pathology*; D008297:Male; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D029842:Phyllanthus emblica; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D032341:Withania
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts
keywords: Bacopa monnieri; Beauveria bassiana; Emblica officinalis; Withania somnifera; age-related macular degeneration; angiogenesis; fermentation