title: The dopamine D3 receptor regulates the effects of methamphetamine on LPS-induced cytokine production in murine mast cells.
abstract: Previous studies have demonstrated that methamphetamine (METH) alter inflammatory and anti-inflammatory cytokine production in the periphery. However, the effect of METH on lipopolysaccharide (LPS)-induced immune responses and its underlying mechanism of action remains unclear. The dopamine D3 receptor (D3R) plays an important role in METH addiction, indicating that the D3R may regulate METH-mediated immune responses. In this study, we examined the effect of METH on mast cell released cytokines in the lungs and thymi of mice stimulated by LPS, and on LPS-induced murine bone marrow-derived mast cells (BMMCs). Moreover, we used D3R-deficient mice to investigate the effect of this receptor on LPS-stimulated mast cell released cytokine production after METH treatment in the lungs and thymi. The effects of a D3R agonist and antagonist on LPS-induced cytokine production after METH treatment in murine BMMCs were also evaluated. METH suppressed LPS-induced cytokine production in the lungs and thymi of wild-type (WT) mice and BMMCs. However, METH did not alter LPS-induced cytokine production in the lungs and thymi of D3R-deficient mice. When BMMCs were treated with the D3R receptor antagonist, NGB2904 hydrochloride (NGB-2904), METH did not alter LPS-induced cytokine production. However, treatment with the D3R agonist, 7-hydroxy-(di-n-propylamino) tetralin (7-OH-DPAT), significantly enhanced the effects of METH on LPS-induced cytokine production. Our results suggest that METH regulates mast cell released cytokines production in an LPS-induced mouse model via the D3R.
authors.affiliation: Department of Immunology and Pathogenic Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China; Forensic Medicine College of Xi'an Jiaotong University, Key Laboratory of the Health Ministry for Forensic Medicine, Key Laboratory of the Ministry of Education for Environment and Genes Related to Diseases, Xi'an 710061, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xue
authors.affiliation: VIP Internal Medicine Department, Lanzhou University Second Hospital, Lanzhou 730030, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Immunology and Pathogenic Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Hui-Xun
authors.initials: HX
authors.lastname: Ren
authors.affiliation: Graduate Teaching and Experiment Centre, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Cardiovascular Surgery, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Immunology and Pathogenic Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Internal Medicine, The First Affiliated Hospital of Medical College of Xi'an Jiao Tong University, Xi'an 710061, China.
authors.forename: Fang-Yuan
authors.initials: FY
authors.lastname: Chen
authors.affiliation: Department of Immunology and Pathogenic Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
authors.forename: Wei-Ying
authors.initials: WY
authors.lastname: Cheng
authors.affiliation: School of Public Security, Northwest University of Politics and Law, Xi'an 710063, China.
authors.forename: Ju-Ping
authors.initials: JP
authors.lastname: Li
authors.affiliation: Department of Immunology and Pathogenic Biology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China. Electronic address: chenyanjiong@126.com.
authors.forename: Yan-Jiong
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Forensic Medicine College of Xi'an Jiaotong University, Key Laboratory of the Health Ministry for Forensic Medicine, Key Laboratory of the Ministry of Education for Environment and Genes Related to Diseases, Xi'an 710061, China. Electronic address: chenteng@mail.xjtu.edu.cn.
authors.forename: Teng
authors.initials: T
authors.lastname: Chen
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology*; D008168:Lung / Q000276:immunology / Q000378:metabolism; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism*; D008694:Methamphetamine / Q000494:pharmacology*; D051379:Mice; D018345:Mice, Knockout; D050637:Receptors, Dopamine D3 / Q000235:genetics / Q000378:metabolism*; D018418:Th2 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D050637:Receptors, Dopamine D3; D008694:Methamphetamine
keywords: Cytokines; Dopamine D3 receptor; LPS; Mast cells; Methamphetamine