title: Electrochemical determination of the anticancer drug taxol at a ds-DNA modified pencil-graphite electrode and its application as a label-free electrochemical biosensor.
abstract: In this study a novel biosensor for determination of taxol is described. The interaction of taxol with salmon-sperm double-stranded DNA (ds-DNA) based on the decreasing of the oxidation signals of guanine and adenine bases was studied electrochemically with a pencil-graphite electrode (PGE) using a differential pulse voltammetry (DPV) method. The decreases in the intensity of the guanine and adenine oxidation signals after interaction with taxol were used as indicator signals for the sensitive determination of taxol. DPV exhibits a linear dynamic range of 2.0×10(-7)-1.0×10(-5) M for taxol with a detection limit of 8.0×10(-8) M. Finally, this modified electrode was used for determination of taxol in some real samples.
authors.affiliation: Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran; Young Researchers Society, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Tajik
authors.affiliation: Department of Chemistry, Shahid Bahonar University of Kerman, P.O. Box 76175-133, Kerman, Iran.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Taher
authors.affiliation: Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. Electronic address: h.beitollahi@yahoo.com.
authors.forename: Hadi
authors.initials: H
authors.lastname: Beitollahi
authors.affiliation: Biotechnology Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
authors.forename: Mosoud
authors.initials: M
authors.lastname: Torkzadeh-Mahani
mesh_terms: D000225:Adenine / Q000737:chemistry; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite; D006147:Guanine / Q000737:chemistry; D006801:Humans; D010084:Oxidation-Reduction; D017239:Paclitaxel / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000652:urine
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D006147:Guanine; D006108:Graphite; D004247:DNA; D000225:Adenine; D017239:Paclitaxel
keywords: Anticancer drug determination; DNA biosensor; Double-stranded DNA; Pencil-graphite electrode; Taxol