title: microRNA-214 functions as a tumor suppressor in human colon cancer via the suppression of ADP-ribosylation factor-like protein 2.
abstract: microRNAs (miRNAs/miRs) are a conserved class of endogenous, short non-coding RNAs that post-transcriptionally regulate the expression of genes involved in diverse cellular processes. miR-214 has been reported to be associated with several cancers, including human colon cancer. However, the function of miR-214 in colon cancer development is poorly understood. In the current study, miR-214 was demonstrated to be downregulated in colon cancer tissues compared with healthy colon tissues. Functional studies showed that miR-214 overexpression results in the inhibition of cell viability, colony formation and proliferation, and the induction of cell apoptosis. ADP-ribosylation factor-like protein 2 (ARL2) is predicted to be a target candidate of miR-214. A luciferase reporter assay, western blot analysis and quantitative polymerase chain reaction were performed, which revealed that miR-214 negatively regulates ARL2 expression by targeting its 3' untranslated region directly. In conclusion, the results of the present study revealed that miR-214 suppresses colon cancer cell growth via the suppression of ARL2, and indicated that miR-214 may present a significant potential therapeutic target for colon cancer.
authors.affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Long
authors.affiliation: Department of Oncology, 421 Hospital of the People's Liberation Army, Guangzhou, Guangdong 510318, P.R. China.
authors.forename: Ben-Fu
authors.initials: BF
authors.lastname: He
authors.affiliation: Department of Emergency, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Huang
authors.affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Guo
authors.affiliation: Department of Geriatrics, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: You-Shuo
authors.initials: YS
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.
authors.forename: Ji-Rong
authors.initials: JR
authors.lastname: Huo
keywords: ADP-ribosylation factor-like protein 2; apoptosis; human colon cancer; miR-214; proliferation