title: Optimum design of amphiphilic polymers bearing hydrophobic groups for both cell surface ligand presentation and intercellular cross-linking.
abstract: Amphiphilic polymers bearing hydrophobic alkyl groups are expected to be applicable for both ligand presentation on the cell surface and intercellular crosslinking. To explore the optimum design for each application, we synthesized eight different acyl-modified dextrans with varying molecular weight, alkyl length, and alkyl modification degree. We found that the behenate-modified polymers retained on the cell surface longer than the palmitate-modified ones. Since the polymers were also modified with biotin, streptavidin can be presented on the cell surface through biotin-streptavidin recognition. The duration of streptavidin on the cell surface is longer in the behenate-modified polymer than the palmitate-modified one. As for the intercellular crosslinking, the palmitate-modified polymers were more efficient than the behenate-modified polymers. The findings in this research will be helpful to design the acyl-modified polymers for the cell surface engineering.
authors.affiliation: a Graduate School of System Life Science , Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395 , Japan.
authors.forename: Masafumi
authors.initials: M
authors.lastname: Takeo
authors.forename: Cuicui
authors.initials: C
authors.lastname: Li
authors.forename: Masayoshi
authors.initials: M
authors.lastname: Matsuda
authors.forename: Hiroko
authors.initials: H
authors.lastname: Nagai
authors.forename: Wataru
authors.initials: W
authors.lastname: Hatanaka
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kishimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D000818:Animals; D001710:Biotin / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008970:Molecular Weight; D010168:Palmitates / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D000067996:RAW 264.7 Cells; D019809:Streptavidin / Q000737:chemistry
chemical_list: D003911:Dextrans; D008024:Ligands; D008565:Membrane Proteins; D010168:Palmitates; D011108:Polymers; D001710:Biotin; D019809:Streptavidin
keywords: amphiphilic polymers; cell surface modification; cellular crosslinker; dextran