title: Direct solid sample analysis with graphite furnace atomic absorption spectrometry—a fast and reliable screening procedure for the determination of inorganic arsenic in fish and seafood.
abstract: Direct solid sample analysis with graphite furnace atomic absorption spectrometry (SS-GF AAS) was investigated initially with the intention of developing a method for the determination of total As in fish and other seafood. A mixture of 0.1% Pd+0.06% Mg+0.06% Triton X-100 was used as the chemical modifier, added in solution over the solid samples, making possible the use of pyrolysis and atomization temperatures of 1200 °C and 2400 °C, respectively. The sample mass had to be limited to 0.25 mg, as the integrated absorbance did not increase further with increasing sample mass. Nevertheless, the recovery of As from several certified reference materials was of the order of 50% lower than the certified value. Strong molecular absorption due to the phosphorus monoxide molecule (PO) was observed with high-resolution continuum source AAS (HR CS AAS), which, however, did not cause any spectral interference. A microwave-assisted digestion with HNO3/H2O2 was also investigated to solve the problem; however, the results obtained for several certified reference materials were statistically not different from those found with direct SS-GF AAS. Accurate values were obtained using inductively coupled plasma mass spectrometry (ICP-MS) to analyze the digested samples, which suggested that organic As compounds are responsible for the low recoveries. HPLC-ICP-MS was used to determine the arsenobetaine (AB) concentration. Accurate results that were not different from the certified values were obtained when the AB concentration was added to the As concentration found by SS-GF AAS for most certified reference materials (CRM) and samples, suggesting that SS-GF AAS could be used as a fast screening procedure for inorganic As determination in fish and seafood.
authors.affiliation: Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: arianezmozinski@gmail.com.
authors.forename: Ariane V
authors.initials: AV
authors.lastname: Zmozinski
authors.affiliation: Department of Analytical Chemistry, Universitat de Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain.
authors.forename: Toni
authors.initials: T
authors.lastname: Llorente-Mirandes
authors.affiliation: Faculdade Dom Bosco de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Isabel C F
authors.initials: ICF
authors.lastname: Damin
authors.affiliation: Department of Analytical Chemistry, Universitat de Barcelona, Martí i Franquès 1-11, E-08028 Barcelona, Spain.
authors.forename: José F
authors.initials: JF
authors.lastname: López-Sánchez
authors.affiliation: Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Instituto Nacional de Ciência e Tecnologia do CNPq, INCT de Energia e Ambiente, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil.
authors.forename: Maria Goreti R
authors.initials: MGR
authors.lastname: Vale
authors.affiliation: Instituto Nacional de Ciência e Tecnologia do CNPq, INCT de Energia e Ambiente, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil; Departamento de Química, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Welz
authors.affiliation: Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Instituto Nacional de Ciência e Tecnologia do CNPq, INCT de Energia e Ambiente, Universidade Federal da Bahia, 40170-115 Salvador, BA, Brazil.
authors.forename: Márcia M
authors.initials: MM
authors.lastname: Silva
mesh_terms: D000818:Animals; D001152:Arsenicals / Q000032:analysis*; D049872:Bivalvia / Q000737:chemistry*; D005396:Fish Products / Q000032:analysis*; D005399:Fishes / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry; D017830:Octoxynol / Q000737:chemistry; D010165:Palladium / Q000737:chemistry; D012015:Reference Standards; D017747:Seafood / Q000032:analysis*; D013054:Spectrophotometry, Atomic / Q000379:methods
chemical_list: D001152:Arsenicals; D010165:Palladium; D017830:Octoxynol; D008274:Magnesium; C038992:arsenobetaine
keywords: Arsenobetaine; Direct solid sample analysis; Graphite furnace AAS; Inorganic arsenic; Seafood