title: Characterization and stress-induced expression analysis of Alfin-like transcription factors in Brassica rapa.
abstract: The Alfin-like (AL) transcription factors (TFs) family is involved in many developmental processes, including the growth and development of roots, root hair elongation, meristem development, etc. However, stress resistance-related function and the regulatory mechanism of these TFs have yet to be elucidated. This study identified 15 Brassica rapa AL (BrAL) TFs from BRAD database, analyzed the sequences and profiled their expression first time in response to Fusarium oxysporum f. sp. conglutinans and Pectobacterium carotovorum subsp. carotovorum in fection, cold, salt and drought stresses in B. rapa. Structural and phylogenetic analyses of 15 BrAL TFs revealed four distinct groups (groups I-IV) with AL TFs of Arabidopsis thaliana. In the expression analyses, ten BrAL TFs showed responsive expression after F. oxysporum f. sp. conglutinans infection, while all BrAL TFs showed responses under cold, salt and drought stresses in B. rapa. Interestingly, ten BrAL TFs showed responses to both biotic and abiotic stress factors tested here. The differentially expressed BrAL TFs thus represent potential resources for molecular breeding of Brassica crops resistant against abiotic and biotic stresses. Our findings will also help to elucidate the complex regulatory mechanism of AL TFs in stress resistance and provide a foundation for further functional genomics studies and applications.
authors.affiliation: Department of Horticulture, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam, 540-950, South Korea.
authors.forename: Md Abdul
authors.initials: MA
authors.lastname: Kayum
authors.forename: Jong-In
authors.initials: JI
authors.lastname: Park
authors.forename: Nasar Uddin
authors.initials: NU
authors.lastname: Ahmed
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Jung
authors.forename: Gopal
authors.initials: G
authors.lastname: Saha
authors.forename: Jong-Goo
authors.initials: JG
authors.lastname: Kang
authors.forename: Ill-Sup
authors.initials: IS
authors.lastname: Nou
mesh_terms: D000595:Amino Acid Sequence; D001937:Brassica / Q000187:drug effects / Q000235:genetics* / Q000382:microbiology; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D003080:Cold Temperature; D055864:Droughts; D005091:Exons; D005670:Fusarium / Q000502:physiology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D016973:Pectobacterium carotovorum / Q000502:physiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000145:classification / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride