title: Genetic diversity and relationship of Hedychium from Northeast India as dissected using PCA analysis and hierarchical clustering.
abstract: Molecular genetic fingerprints of eleven Hedychium species from Northeast India were developed using PCR based markers. Fifteen inter-simple sequence repeats (ISSRs) and five amplified fragment length polymorphism (AFLP) primers produced 547 polymorphic fragments. Positive correlation (r = 0.46) was observed between the mean genetic similarity and genetic diversity parameters at the inter-species level. AFLP and ISSR markers were able to group the species according to its altitude and intensity of flower aroma. Cophenetic correlation coefficients between the dendrogram and the original similarity matrix were significant for ISSR (r = 0.89) compared to AFLP (r = 0.83) markers. This genetic characterization of Hedychium from Northeast India contributes to the knowledge of genetic structure of the species and can be used to define strategies for their conservation and management.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781 039, India.
authors.forename: Supriyo
authors.initials: S
authors.lastname: Basak
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781 039, India.
authors.forename: Aadi Moolam
authors.initials: AM
authors.lastname: Ramesh
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781 039, India.
authors.forename: Vigya
authors.initials: V
authors.lastname: Kesari
authors.affiliation: M S Swaminathan Research Foundation, 3rd Cross Street, Taramani Institutional area, Chennai 600 113, India.
authors.forename: Ajay
authors.initials: A
authors.lastname: Parida
authors.affiliation: Department of Environmental Science, Tezpur University, Assam 784 028, India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Mitra
authors.affiliation: Department of Biotechnology, Indian Institute of Technology, Guwahati, Assam 781 039, India.
authors.forename: Latha
authors.initials: L
authors.lastname: Rangan
keywords: AFLP; AFLP, amplified fragment length polymorphism; DNA, deoxyribonucleic Acid; GUBH, Gauhati university botanical herbarium; Genetic diversity; HCA, hierarchical clustering; Hedychium; ISSR, inter- simple sequence repeat; MI, marker index; NE, North east; NTSYS, numerical taxonomy system; Northeast India; PCA; PCA, principal component analysis; PCR, polymerase chain reaction; PIC, polymorphic information content; UPGMA, unweighted pair-group arithmetic average.