title: Human umbilical cord blood-derived mesenchymal stem cells promote regeneration of crush-injured rat sciatic nerves.
abstract: Several studies have demonstrated that human umbilical cord blood-derived mesenchymal stem cells can promote neural regeneration following brain injury. However, the therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells in guiding peripheral nerve regeneration remain poorly understood. This study was designed to investigate the effects of human umbilical cord blood-derived mesenchymal stem cells on neural regeneration using a rat sciatic nerve crush injury model. Human umbilical cord blood-derived mesenchymal stem cells (1 × 10(6)) or a PBS control were injected into the crush-injured segment of the sciatic nerve. Four weeks after cell injection, brain-derived neurotrophic factor and tyrosine kinase receptor B mRNA expression at the lesion site was increased in comparison to control. Furthermore, sciatic function index, Fluoro Gold-labeled neuron counts and axon density were also significantly increased when compared with control. Our results indicate that human umbilical cord blood-derived mesenchymal stem cells promote the functional recovery of crush-injured sciatic nerves.
authors.affiliation: Department of Craniofacial Structure & Functional Biology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Sung
authors.affiliation: Department of Occupation and Environment, Postgraduate College of Medicine, Konkuk University, Choong-ju, Republic of Korea.
authors.forename: Hun Jong
authors.initials: HJ
authors.lastname: Jung
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea ; Department of Dentistry, Oral and Maxillofacial Surgery, Ajou University School of Medicine, Suwon, Republic of Korea.
authors.forename: Kang-Mi
authors.initials: KM
authors.lastname: Pang
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Yoo
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Alrashdan
authors.affiliation: Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
authors.forename: Soung-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Jahng
authors.affiliation: Department of Craniofacial Structure & Functional Biology, School of Dentistry, Seoul National University, Seoul, Republic of Korea ; Department of Oral & Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Republic of Korea ; Dental Research Institute, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
keywords: Fluoro Gold; crush injury; human umbilical cord blood-derived mesenchymal stem cells; neural regeneration; peripheral nerve regeneration; regeneration; sciatic nerve; stem cells