title: Crucial role of the protein corona for the specific targeting of nanoparticles.
abstract: OBJECTIVE
  We aimed to investigate the physicochemical effects of superparamagnetic iron oxide nanoparticles (SPIONs) on the composition of the protein corona and their correspondence toxicological issues.
  
  
  METHODS
  SPIONs of different sizes and surface charges were exposed to fetal bovine serum. The structure/composition and biological effects of the protein corona-SPION complexes were probed.
  
  
  CONCLUSIONS
  The affinity and level of adsorption of specific proteins is strongly dependent on the size and surface charge of the SPIONs. In vivo experiments on the mouse blood-brain barrier model revealed that nontargeted SPIONs containing specific proteins will enter the brain endothelial barrier cells.
  
  
  CONCLUSIONS
  Some commercially available nanoparticles used for target-specific applications may have unintended uptake in the body (e.g., brain tissue) with potential cytotoxity.
authors.affiliation: Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Morteza
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Sara
authors.initials: S
authors.lastname: Sheibani
authors.forename: Abbas S
authors.initials: AS
authors.lastname: Milani
authors.forename: Farhad
authors.initials: F
authors.lastname: Rezaee
authors.forename: Maxime
authors.initials: M
authors.lastname: Gauberti
authors.forename: Rassoul
authors.initials: R
authors.lastname: Dinarvand
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Vali
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000378:metabolism; D002417:Cattle; D003287:Contrast Media / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D011506:Proteins; C065507:ferumoxides
keywords: LC-MS/MS; SPIONs; protein corona; surface charge