title: Pharmacokinetics of Gd(DO3A-Lys) and MR imaging studies in an orthotopic U87MG glioma tumor model.
abstract: Pharmacokinetics of Gd(DO3A-Lys), a macrocyclic gadolinium-based magnetic resonance imaging (MRI) contrast agent functionalized with a lysine derivative, was studied in Wistar rats. Kinetic data were fitted using a two-compartment model and revealed Gd(DO3A-Lys) to have a distribution half-life, t1/2 (α), of 1.3 min, an elimination half-life, t1/2 (β), of 24.9 min and a large volume of distribution, VD , of 0.49 L/kg indicative of the agent being able to rapidly distribute into tissues and organs. Contrast-enhanced magnetic resonance angiography (CE-MRA) in an orthotopic U87MG glioma mouse model demonstrated considerable enhancement of both the tumor and surrounding vasculature after intravenous administration of Gd(DO3A-Lys). Applying dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the glioma of different sizes further showed distinct uptake characteristics and patterns of enhancement, which suggests the potential for differentiating changes at different stages of tumor growth. Our results indicate that Gd(DO3A-Lys) could be a promising candidate for glioma MR imaging.
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.
authors.forename: Prashant
authors.initials: P
authors.lastname: Chandrasekharan
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.|The Lee Kong Chian School of Medicine, Nanyang Technological University, 50 Nanyang Drive, Singapore, 637553.
authors.forename: Chang-Tong
authors.initials: CT
authors.lastname: Yang
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.
authors.forename: Fatima Ali
authors.initials: FA
authors.lastname: Nasrallah
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.
authors.forename: Hui Chien
authors.initials: HC
authors.lastname: Tay
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.|Clinical Imaging Research Centre, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive #B1-01, Singapore, 117599.
authors.forename: Kai-Hsiang
authors.initials: KH
authors.lastname: Chuang
authors.affiliation: Laboratory of Molecular Imaging, Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios, Singapore, 138667.|Clinical Imaging Research Centre, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive #B1-01, Singapore, 117599.
authors.forename: Edward G
authors.initials: EG
authors.lastname: Robins
mesh_terms: D000818:Animals; D003287:Contrast Media / Q000737:chemistry / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D005682:Gadolinium / Q000737:chemistry / Q000493:pharmacokinetics*; D005910:Glioma / Q000098:blood supply* / Q000175:diagnosis; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry / Q000493:pharmacokinetics*; D008239:Lysine / Q000737:chemistry; D018810:Magnetic Resonance Angiography / Q000379:methods*; D008297:Male; D009942:Organometallic Compounds / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar
chemical_list: C520644:1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid; D003287:Contrast Media; D006573:Heterocyclic Compounds, 1-Ring; D009942:Organometallic Compounds; D005682:Gadolinium; D008239:Lysine
keywords: Gadolinium chelate; contrast agent; magnetic resonance imaging (MRI); orthotopic glioma model; pharmacokinetics; tumor diagnosis