title: Topical microemulsion containing Punica granatum extract: its control over skin erythema and melanin in healthy Asian subjects.
abstract: BACKGROUND
  Punica granatum is apotent source of polyphenolic compounds with strong free radicals scavenging activity. The skin lightening effects of Punica granatum are assumed due to ellagic acid which acts by chelating copper at the active site of tyrosinase.
  
  
  OBJECTIVE
  To explore a topical microemulsion (O/W) of pomegranate (Punica granatum) extract for its control on skin erythema and melanin.
  
  
  METHODS
  Microemulsions were formulated using a polysorbate surfactant (Tween 80(®)) along with cosurfactant (propylene glycol) and were characterized regarding their stability. The placebo microemulsion (without extract) and the active microemulsion (containing Punica extract) were applied in a split face fashion by the volunteers (n = 11) for a period of 12 weeks. Skin erythema and melanin were measured at baseline and after every 15 days to determine any effect produced by these formulations.
  
  
  RESULTS
  Active formulation showed a significant impact on skin erythema and melanin (p < 0.05).
  
  
  CONCLUSIONS
  This study reveals that a suitable topical formulation like microemulsion could employ the Punica granatum extract for conditions where elevated skin melanin and erythema have significantly prone skin physiology.
authors.affiliation: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Rashida
authors.initials: R
authors.lastname: Parveen
authors.affiliation: Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Naveed
authors.initials: N
authors.lastname: Akhtar
authors.affiliation: Faculty of Pharmacy, University of Central Punjab, Lahore, Pakistan.
authors.forename: Tariq
authors.initials: T
authors.lastname: Mahmood
keywords: Punica granatum extract; antioxidant; erythema; melanin; microemulsion