title: Molecular and cellular profiles of the resolution phase in a damage-associated molecular pattern (DAMP)-mediated peritonitis model and revelation of leukocyte persistence in peritoneal tissues.
abstract: Models of microbe-elicited peritonitis have been invaluable to identify mechanisms underlying inflammation resolution, but whether resolution mechanisms differ from an inflammatory agent to another has not been determined. Thus, we analyzed the cellular and molecular components of the resolution phase of non-microbe-induced inflammation. In thioglycollate (TG)-induced peritonitis, resolution started at 12 h (Tmax) and displayed a 22 h resolution interval (Ri). During resolution, lipoxin A4, resolvin (Rv) D1 and RvD2, protectin D1 (PD1), and maresin 1 (MaR1) were transiently produced while RvD5 was continually generated. In addition, docosahexaenoic acid (DHA)-derived mediators were produced to a higher extent than in microbial peritonitis. We also investigated leukocyte infiltration and clearance in peritoneal tissues surrounding the inflammatory site. In the omentum, resolution parameters, neutrophil apoptosis, and efferocytosis were similar to those of the peritoneal cavity. However, we noticed long-term persistence of M2-polarized macrophages and B-lymphocytes in the omentum after TG administration, whereas zymosan injection caused M1/M2-macrophage and T-lymphocyte persistence regardless of the magnitude of the inflammatory response. Our study indicates that some aspects of resolution are shaped in a stimulus-specific manner, and it ultimately argues that the tissues surrounding the inflammatory site must also be considered to address the inflammatory response globally.
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Lastrucci
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Baillif
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Annie
authors.initials: A
authors.lastname: Behar
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Talal
authors.initials: T
authors.lastname: Al Saati
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Dubourdeau
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France isabelle.maridonneau-parini@ipbs.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Maridonneau-Parini
authors.affiliation: *Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale (IPBS), Département Tuberculosis and Infection Biology, Toulouse, France; Université de Toulouse; Université Paul Sabatier, UPS, IPBS, Toulouse, France; Ambiotis-SAS, Toulouse, France; and INSERM/UPS-US006/CREFRE, Service d'Histopathologie, CHU Purpan, Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Cougoule
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D002478:Cells, Cultured; D004281:Docosahexaenoic Acids / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D007124:Immunoenzyme Techniques; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D007962:Leukocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008055:Lipids / Q000032:analysis; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D009852:Omentum / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism*; D010587:Phagocytosis / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013864:Thioglycolates / Q000633:toxicity*; D015054:Zymosan / Q000633:toxicity
chemical_list: D008055:Lipids; D012333:RNA, Messenger; D013864:Thioglycolates; C487679:protectin D1; C518399:resolvin D1; D004281:Docosahexaenoic Acids; D015054:Zymosan
keywords: bioactive lipid mediators; macrophage polarization; omentum