title: Protective effects of Isofraxidin against lipopolysaccharide-induced acute lung injury in mice.
abstract: Acute lung injury (ALI) is a life-threatening disease characterized by serious lung inflammation and increased capillary permeability, which presents a high mortality worldwide. Isofraxidin (IF), a Coumarin compound isolated from the natural medicinal plants such as Sarcandra glabra and Acanthopanax senticosus, has been reported to have definite anti-bacterial, anti-oxidant, and anti-inflammatory activities. However, the effects of IF against lipopolysaccharide-induced ALI have not been clarified. The aim of the present study is to explore the protective effects and potential mechanism of IF against LPS-induced ALI in mice. In this study, We found that pretreatment with IF significantly lowered LPS-induced mortality and lung wet-to-dry weight (W/D) ratio and reduced the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2) in serum and bronchoalveolar lavage fluid (BALF). We also found that total cells, neutrophils and macrophages in BALF, MPO activity in lung tissues were markedly decreased. Besides, IF obviously inhibited lung histopathological changes and cyclooxygenase-2 (COX-2) protein expression. These results suggest that IF has a protective effect against LPS-induced ALI, and the protective effect of IF seems to result from the inhibition of COX-2 protein expression in the lung, which regulates the production of PGE2.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China. Electronic address: niuxf@mail.xjtu.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Niu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China. Electronic address: liwf@mail.xjtu.edu.cn.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Mu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Huani
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yao
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D003374:Coumarins / Q000494:pharmacology* / Q000627:therapeutic use*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D003374:Coumarins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha; C577686:interleukin-6, mouse; C008182:isofraxidin; C077623:Ptgs2 protein, mouse; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: Acute lung injury; Cyclooxygenase-2; Inflammatory cytokines; Isofraxidin; Prostaglandin E(2)