title: Unravelling plant molecular machineries through affinity purification coupled to mass spectrometry.
abstract: Rather than functioning independently, proteins tend to work in concert with each other and with other macromolecules to form macromolecular complexes. Affinity purification coupled to mass spectrometry (AP-MS) can lead to a better understanding of the cellular functions of these complexes. With the development of easy purification protocols and ultra-sensitive MS, AP-MS is currently widely used for screening co-complex membership in plants. Studying complexes in their developmental context through the isolation of specific organs and tissues has now become feasible. Besides, the tagged protein can be employed for probing other interactions like protein-DNA and protein-RNA interactions. With the tools at hand, protein-centred interaction studies will greatly improve our knowledge of how plant cells wire their functional components in relation to their function.
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium; CropDesign N.V., Technologiepark 21, B-9052 Ghent, Belgium.
authors.forename: Maarten
authors.initials: M
authors.lastname: Dedecker
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium.
authors.forename: Jelle
authors.initials: J
authors.lastname: Van Leene
authors.affiliation: Department of Plant Systems Biology, VIB, Technologiepark 927, B-9052 Ghent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium. Electronic address: geert.dejaeger@psb.vib-ugent.be.
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
mesh_terms: D002846:Chromatography, Affinity; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins