title: Stereospecific phosphorylation by the central mitotic kinase Cdk1-cyclin B.
abstract: The cis vs trans conformation, or shape, of phosphoserine-proline (pSer-Pro), a prevalent motif in cell cycle proteins, may play a significant role in regulating mitosis. We demonstrate that Cdk1-cyclin B, the central mitotic kinase, is specific for the trans conformation, not cis, of synthetic, locked Ser-Pro 11-residue peptide substrates, using LC-MSMS detection and sequencing of phosphorylated products. This substrate stereospecificity may contribute an additional level of mitotic regulation.
authors.affiliation: Virginia Tech, Department of Chemistry, Blacksburg, Virginia 24061, United States.
authors.forename: Felicia A
authors.initials: FA
authors.lastname: Etzkorn
authors.affiliation: Virginia Tech, Department of Chemistry, Blacksburg, Virginia 24061, United States.
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
mesh_terms: D016203:CDC2 Protein Kinase; D056744:Cyclin B1 / Q000737:chemistry* / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000737:chemistry* / Q000378:metabolism*; D008938:Mitosis; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D019696:Peptidylprolyl Isomerase / Q000378:metabolism; D010766:Phosphorylation; D011487:Protein Conformation; D060327:Solid-Phase Synthesis Techniques; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: C534699:CCNB1 protein, human; D056744:Cyclin B1; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D010455:Peptides; D016203:CDC2 Protein Kinase; C528899:CDK1 protein, human; D018844:Cyclin-Dependent Kinases; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase