title: Molecular and cellular approach in the study of antioxidant/pro-oxidant properties of Micromeria croatica (Pers.) Schott.
abstract: Phytochemical composition and antioxidant activity of three wild populations of endemic Illyric-Balcanic species Micromeria croatica (Pers.) Schott have been evaluated with respect to plant organ and growing location. Multivariate analysis (principal component analysis) was performed to visualise (dis-)similarity among samples and identify the correlations between phytochemical variables that explain the most variability. The tested leaf extract from BaČić kuk locality exhibited protective effects against reactive oxygen species-induced damage of DNA and inhibition of lipid peroxidation, while it caused oxidative degradation of protein in the bovine serum albumin assay at higher concentrations. This extract also exhibited cytotoxic activity and facilitated the formation of reactive oxygen species in the HEp2 cell line, in a dose-dependent manner.
authors.affiliation: a Department of Molecular Biology , Institute 'Ruđer Bošković , BijeniČka c. 54, P.O. Box 180, 10000 Zagreb , Croatia.
authors.forename: Dunja
authors.initials: D
authors.lastname: Šamec
authors.forename: Jiri
authors.initials: J
authors.lastname: Gruz
authors.forename: Ksenija
authors.initials: K
authors.lastname: Durgo
authors.forename: Dario
authors.initials: D
authors.lastname: Kremer
authors.forename: Ivan
authors.initials: I
authors.lastname: Kosalec
authors.forename: Lidija Valek
authors.initials: LV
authors.lastname: Žulj
authors.forename: Sanja
authors.initials: S
authors.lastname: Martinez
authors.forename: Branka
authors.initials: B
authors.lastname: Salopek-Sondi
authors.forename: Jasenka
authors.initials: J
authors.lastname: Piljac-Žegarac
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D045744:Cell Line, Tumor; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D017382:Reactive Oxygen Species
keywords: Micromeria croatica (Pers.) Schott; antioxidant; cytotoxicity; polyphenols; pro-oxidant