title: Expression of poly(ADP-ribose) polymerase in bone regeneration.
abstract: Poly(ADP-ribose) polymerase (PARP) is a 116kDa enzyme catalysing the synthesis of ADP-ribose polymers from NAD+. PARP is activated in response to DNA strand breaks and plays a critical role in the maintenance of genomic integrity. However, considering its role also in transcription, proliferation as well as apoptosis in biological process, in the present study the role of PARP in bone regeneration was evaluated, in particular in bone cell proliferation and differentiation processes. Thus, formalin fixed paraffin embedded specimens of 10 human bone samples after sinus lift were collected and investigated by immunohistochemistry using a mouse monoclonal anti-human PARP antibody. PARP was expressed in cells with morphological features of osteoblasts in the areas of new bone formation at the junction between mineralized and unmineralized tissue, between osteoid tissue and bone. Few osteoclasts were observed and showed only focal nuclear expression of PARP, while osteocytes showed no positivity for PARP. Our data showed an overall involvement of PARP enzyme in human bone tissues, in particular during bone regeneration process.
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lo Muzio
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Pannone
authors.affiliation: Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Santarelli
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lo Russo
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: A
authors.initials: A
authors.lastname: De Lillo
authors.affiliation: Department of Pathologic Anatomy and Histopathology, Marche Polytechnic University, Ancona, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Rubini
authors.affiliation: Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Bambini
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Bufo
authors.affiliation: Department of Department of Clinical and Experimental Medicine, Foggia University, Foggia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Dioguardi
authors.affiliation: Department of Clinic Specialistic and Stomatological Sciences, Marche Polytechnic University, Ancona, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Procaccini
mesh_terms: D017209:Apoptosis; D001861:Bone Regeneration; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D010006:Osteoblasts / Q000201:enzymology; D011065:Poly(ADP-ribose) Polymerases / Q000032:analysis* / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D011065:Poly(ADP-ribose) Polymerases