title: Isolation of isochlorogenic acid isomers in flower buds of Lonicera japonica by high-speed counter-current chromatography and preparative high performance liquid chromatography.
abstract: An efficient method was established by HSCCC combined with prep-HPLC for separating isochlorogenic acid isomers from flower buds of Lonicera japonica. The partially purified sample from the methanol extract of flower buds of L. japonica by silica gel column was separated by HSCCC to result in a fraction containing two isochlorogenic acid isomers. The fraction was further isolated by prep-HPLC to yield isochlorogenic acid A and isochlorogenic acid C with purities of 98% and 96%, and the total recoveries at 80.1% and 79.8%, respectively. The chemical structures of isochlorogenic acid isomers were confirmed by electrospray ionization mass spectrometry (ESI-MS) and (1)H nuclear magnetic resonance (NMR).
authors.affiliation: Taishan Scholar-Distinguished Experts Position, Key Disciplines on Analysis of Traditional Chinese Medicine, Shandong Key Laboratory of Chinese Medicine Quality Standard Research, Shandong Academy of Chinese Medicine, 7 West of Yanzishan Street, Jinan, Shandong 250014, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Taishan Scholar-Distinguished Experts Position, Key Disciplines on Analysis of Traditional Chinese Medicine, Shandong Key Laboratory of Chinese Medicine Quality Standard Research, Shandong Academy of Chinese Medicine, 7 West of Yanzishan Street, Jinan, Shandong 250014, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Taishan Scholar-Distinguished Experts Position, Key Disciplines on Analysis of Traditional Chinese Medicine, Shandong Key Laboratory of Chinese Medicine Quality Standard Research, Shandong Academy of Chinese Medicine, 7 West of Yanzishan Street, Jinan, Shandong 250014, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Taishan Scholar-Distinguished Experts Position, Key Disciplines on Analysis of Traditional Chinese Medicine, Shandong Key Laboratory of Chinese Medicine Quality Standard Research, Shandong Academy of Chinese Medicine, 7 West of Yanzishan Street, Jinan, Shandong 250014, China.
authors.forename: Bonian
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Daijie
authors.initials: D
authors.lastname: Wang
authors.affiliation: Shandong Analysis and Test Center, Shandong Academy of Sciences, 19 Keyuan Street, Jinan, Shandong 250014, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Duan
authors.affiliation: Taishan Scholar-Distinguished Experts Position, Key Disciplines on Analysis of Traditional Chinese Medicine, Shandong Key Laboratory of Chinese Medicine Quality Standard Research, Shandong Academy of Chinese Medicine, 7 West of Yanzishan Street, Jinan, Shandong 250014, China. Electronic address: yuzys@sohu.com.
authors.forename: Zongyuan
authors.initials: Z
authors.lastname: Yu
mesh_terms: D002726:Chlorogenic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003377:Countercurrent Distribution / Q000379:methods*; D035264:Flowers / Q000737:chemistry*; D007536:Isomerism; D031239:Lonicera / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010936:Plant Extracts; C469310:isochlorogenic acid; D002726:Chlorogenic Acid
keywords: Flower buds of Lonicera japonica; High-speed counter-current chromatography; Isochlorogenic acid A; Isochlorogenic acid C; Preparative high performance liquid chromatography