title: Chromatographic adsorption of serum albumin and antibody proteins in cryogels with benzyl-quaternary amine ligands.
abstract: The preparation and characterization of mixed-mode adsorbents for a typical separation purpose are of great importance in bioseparation areas. In this work, we prepared a new monolithic cryogel with a combination of ion-exchange and hydrophobic functions by employing benzyl-quaternary amine groups. The fundamental cryogel properties, protein equilibrium adsorption isotherm and chromatographic adsorption in the cryogel were measured experimentally. The results showed that, by using bovine serum album as the model protein, the dual functional cryogel has protein binding capability even in salt solution and the buffer with pH close or below the protein isoelectric point due to both the electrostatic and hydrophobic interactions. A capillary-based adsorption model was developed, which provided satisfied insights of the microstructure, axial dispersion, mass transfer as well as protein adsorption characteristics within the cryogel bed. The chromatographic isolation of bioactive proteins from rabbit blood serum was carried out by the cryogel. Immunoglobulin G antibody with a purity of 98.2% and albumin with a purity of 96.8% were obtained, indicating that the cryogel could be an interesting and promising adsorbent in bioseparation areas.
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China.
authors.forename: Junxian
authors.initials: J
authors.lastname: Yun
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China.
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Cheng
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Ye
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China.
authors.forename: Shaochuan
authors.initials: S
authors.lastname: Shen
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China.
authors.forename: Gensheng
authors.initials: G
authors.lastname: Yang
authors.affiliation: State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Changwang Road 18, Hangzhou 310032, China. Electronic address: yaokj@zjut.edu.cn.
authors.forename: Kejian
authors.initials: K
authors.lastname: Yao
authors.affiliation: Department of Biotechnology, Lund University, P.O. Box 124, SE-22100 Lund, Sweden.
authors.forename: Harald
authors.initials: H
authors.lastname: Kirsebom
authors.affiliation: Department of Chemical and Biological Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, China.
authors.forename: Dong-Qiang
authors.initials: DQ
authors.lastname: Lin
authors.affiliation: Department of Chemical and Biological Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Guan
authors.affiliation: Department of Chemical and Biological Engineering, Zhejiang University, Zheda Road 38, Hangzhou 310027, China. Electronic address: yaosj@zju.edu.cn.
authors.forename: Shan-Jing
authors.initials: SJ
authors.lastname: Yao
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D059985:Cryogels / Q000138:chemical synthesis / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D007074:Immunoglobulin G / Q000737:chemistry*; D008024:Ligands; D008970:Molecular Weight; D011137:Polystyrenes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D011817:Rabbits; D012709:Serum Albumin / Q000737:chemistry*; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D059985:Cryogels; D007074:Immunoglobulin G; D008024:Ligands; D011137:Polystyrenes; D000644:Quaternary Ammonium Compounds; D012709:Serum Albumin; D000099166:Polyacrylamides; C114826:poly(vinylbenzyltrimethylammonium chloride)
keywords: Adsorption; Bioseparation; Chromatography; Cryogel; Mathematical modeling