title: Sensing and inhibition of amyloid-β based on the simple luminescent aptamer-ruthenium complex system.
abstract: Aggregation of amyloid-β (Aβ) peptide has been known to be pathologically associated with Alzheimer and dementia diseases. Amyloid-β fibrils serve as an important target for the drugs development and diagnosis of neurodegenerative diseases. Herein, we report a new [Ru(dmbpy)(dcbpy)dppz)] complex (dmbpy; 4,4'-dimethyl-2,2'-bipyridine, dcbpy; 4,4'-dicorboxy-2,2'-bipyridine, dppz; dipyridophenazine) intercalated aptamer based recognition of amyloid-β. Interestingly, aforementioned Ru(II) complex shows weak luminescence intensity in the aqueous medium but it shows strong luminescence intensity in the presence of RNA aptamer. Upon addition of amyloid-β monomers, the luminescence intensity of Ru(II) complex is reduced due to the strong interaction of aptamer with amyloid-β monomer/small oligomers. Furthermore, present study implies that our system has ability to inhibit the formation of amyloid-β fibrils, which is confirmed from the AFM morphological structures in the absence and presence of aptamer. This work may contribute the rapid diagnosis and inhibition of amyloid-β aggregation in the clinical applications.
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India; Department of Chemistry, VV College of Engineering, Tisaiyanvilai, Tamil Nadu, India.
authors.forename: Eththilu
authors.initials: E
authors.lastname: Babu
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India; Graduate School of EEWS, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
authors.forename: Paulpandian
authors.initials: P
authors.lastname: Muthu Mareeswaran
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India; Department of Physical Science, Bannari Amman Institute of Technology, Sathiyamangalam, Tamil Nadu, India.
authors.forename: Veerasamy
authors.initials: V
authors.lastname: Sathish
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
authors.forename: Subramanian
authors.initials: S
authors.lastname: Singaravadivel
authors.affiliation: Department of Physical Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu, India. Electronic address: rajagopalseenivasan@yahoo.com.
authors.forename: Seenivasan
authors.initials: S
authors.lastname: Rajagopal
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D049449:Luminescence; D018625:Microscopy, Atomic Force; D009942:Organometallic Compounds / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D052157:Aptamers, Nucleotide; D009942:Organometallic Compounds; D012428:Ruthenium
keywords: Amyloid-β; Amyloid-β fibrils; Aptamers; Biosensors; Luminescence; Ruthenium(II) complexes