title: Evaluation of mold exposure in cystic fibrosis patients' dwellings and allergic bronchopulmonary risk.
abstract: Very few studies have been conducted on cystic fibrosis (CF) patients' exposure to the indoor environment and, to our knowledge, there are no studies dealing with the link between specific fungal environmental exposure at home and fungal colonization resulting in allergic bronchopulmonary aspergillosis (ABPA). Fungal exposure of CF adult patients with ABPA (n=4) with fungal sensitization (n=7) and with no ABPA (n=5) was assessed in 16 homes by dust sampling with electrostatic dust fall collectors (EDCs). Aspergillus fumigatus was specifically quantified by real-time quantitative polymerase chain reactions (qPCRs), and A. fumigatus DNA concentrations were significantly higher in homes of ABPA patients (p<0.001). Results indicate that indoor fungal contamination could be a factor favoring ABPA and suggest that environmental surveys could help in preventing fungal risk in CF patients.
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, University Hospital, Besançon, France.
authors.forename: Steffi
authors.initials: S
authors.lastname: Rocchi
authors.affiliation: Pneumology Department, University Hospital, Besançon, France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Richaud-Thiriez
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, University Hospital, Besançon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Barrera
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, University Hospital, Besançon, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Grenouillet
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Pneumology Department, University Hospital, Besançon, France.
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Dalphin
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, University Hospital, Besançon, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Millon
authors.affiliation: Chrono-Environnement UMR 6249 CNRS, Franche-Comté University, Besançon, France; Parasitology-Mycology Department, University Hospital, Besançon, France. Electronic address: gabriel.reboux@univ-fcomte.fr.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Reboux
mesh_terms: D000328:Adult; D016902:Air Pollution, Indoor / Q000009:adverse effects / Q000032:analysis / Q000517:prevention & control; D000946:Antigens, Fungal / Q000032:analysis; D001229:Aspergillosis, Allergic Bronchopulmonary / Q000150:complications / Q000175:diagnosis / Q000382:microbiology / Q000517:prevention & control; D001232:Aspergillus fumigatus / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology; D004271:DNA, Fungal / Q000032:analysis; D004784:Environmental Monitoring / Q000379:methods; D005260:Female; D005602:France; D006801:Humans; D019570:Inhalation Exposure / Q000009:adverse effects / Q000032:analysis / Q000517:prevention & control; D008297:Male
chemical_list: D000946:Antigens, Fungal; D004271:DNA, Fungal
keywords: Allergic bronchopulmonary aspergillosis (ABPA); Aspergillus fumigatus; Cystic fibrosis (CF); Dwellings; Real-time quantitative polymerase chain reactions (qPCRs)