title: Recommendations to design environmental monitoring in the European bullhead, Cottus sp., based on reproductive cycle and immunomarker measurement.
abstract: European bullhead is a relevant fish species to assess adverse effects of environmental stress on wild fish. Nevertheless, their complex reproductive cycle is very different between sites and could interfere with many physiological processes. Thus, prior to use biomarker to statute on environmental quality of rivers, we wanted to characterize reproductive profile (spawn number, GSI, gonad development). The major results demonstrated that the two types of reproductive cycle shown were strongly correlated to water temperature variation. In a second time, even if innate immunomarkers are highly relevant on biomonitoring program, hormonal variation seems to impact severely their responses. Thus, the link between reproductive status and immune activity (leucocyte distribution, cellular mortality, respiratory burst, phagocytosis activity) must also be study. Nonetheless, in the present work, immune capacities seems to be more correlated with season and environmental factors than reproduction.
authors.affiliation: Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 (INERIS-Université Reims Champagne-Ardenne, Université du Havre) SEBIO Stress environnementaux et Biosurveillance des milieux aquatiques, B.P. 2, 60550 Verneuil-en-Halatte, France; Université Reims Champagne-Ardenne, UMR-I 02 SEBIO, Moulin de la Housse, B.P. 1039, 51687 Reims, France. Electronic address: anne.bado-nilles@ineris.fr.
authors.forename: Anne
authors.initials: A
authors.lastname: Bado-Nilles
authors.affiliation: Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 (INERIS-Université Reims Champagne-Ardenne, Université du Havre) SEBIO Stress environnementaux et Biosurveillance des milieux aquatiques, B.P. 2, 60550 Verneuil-en-Halatte, France; Université du Havre, UMR-I 02 SEBIO, Université du Havre, BP540, 76058 Le Havre, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Villeret
authors.affiliation: Université Reims Champagne-Ardenne, UMR-I 02 SEBIO, Moulin de la Housse, B.P. 1039, 51687 Reims, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Geffard
authors.affiliation: Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 (INERIS-Université Reims Champagne-Ardenne, Université du Havre) SEBIO Stress environnementaux et Biosurveillance des milieux aquatiques, B.P. 2, 60550 Verneuil-en-Halatte, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Palluel
authors.affiliation: Office National de l'Eau et des Milieux Aquatiques (ONEMA), DiR1 Nord Ouest, 2rue de Strasbourg, 60200 Compiègne, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Blanchard
authors.affiliation: Office National de l'Eau et des Milieux Aquatiques (ONEMA), DiR1 Nord Ouest, 2rue de Strasbourg, 60200 Compiègne, France.
authors.forename: Cindy
authors.initials: C
authors.lastname: Le Rohic
authors.affiliation: Office National de l'Eau et des Milieux Aquatiques (ONEMA), DiR1 Nord Ouest, 2rue de Strasbourg, 60200 Compiègne, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Besson
authors.affiliation: Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 (INERIS-Université Reims Champagne-Ardenne, Université du Havre) SEBIO Stress environnementaux et Biosurveillance des milieux aquatiques, B.P. 2, 60550 Verneuil-en-Halatte, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Porcher
authors.affiliation: Université du Havre, UMR-I 02 SEBIO, Université du Havre, BP540, 76058 Le Havre, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Minier
authors.affiliation: Institut National de l'Environnement Industriel et des Risques (INERIS), UMR-I 02 (INERIS-Université Reims Champagne-Ardenne, Université du Havre) SEBIO Stress environnementaux et Biosurveillance des milieux aquatiques, B.P. 2, 60550 Verneuil-en-Halatte, France.
authors.forename: Wilfried
authors.initials: W
authors.lastname: Sanchez
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D005060:Europe; D010473:Perciformes / Q000502:physiology*; D012098:Reproduction; D045483:Rivers; D012621:Seasons; D013696:Temperature; D014876:Water Pollution / Q000032:analysis* / Q000706:statistics & numerical data
chemical_list: D015415:Biomarkers
keywords: Biomonitoring; Fish; Immune fluctuation; Reproduction status