title: Ultrasound-mediated method for rapid delivery of nano-particles into cells for intracellular surface-enhanced Raman spectroscopy and cancer cell screening.
abstract: Surface-enhanced Raman spectroscopy (SERS) is a powerful technology for providing finger-printing information of cells. A big challenge has been the long time duration and inefficient uptake of metal nano-particles into living cells as substrate for SERS analysis. Herein, a simple method (based on ultrasound) for the rapid transfer of silver nanoparticles (NPs) into living cells for intracellular SERS spectroscopy was presented. In this study, the ultrasound-mediated method for NP delivery overcame the shortcoming of 'passive uptake', and achieved quick acquisition of reproducible SERS spectra from living human nasopharyngeal carcinoma cell lines (C666 and CNE1) and normal nasopharyngeal cell line (NP69). Tentative assignment of the Raman bands in the measured SERS spectra showed cancer cell specific biomolecular differences, including significantly lower DNA concentrations and higher protein concentrations in cancerous nasopharyngeal cells as compared to those of normal cells. Combined with PCA-LDA multivariate analysis, ultrasound-mediated cell SERS spectroscopy differentiated the cancerous cells from the normal nasopharyngeal cells with high diagnostic accuracy (98.7%), demonstrating great potential for high-throughput cancer cell screening applications.
authors.affiliation: Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, People's Republic of China.
authors.forename: Shangyuan
authors.initials: S
authors.lastname: Feng
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Li
authors.forename: Guannan
authors.initials: G
authors.lastname: Chen
authors.forename: Duo
authors.initials: D
authors.lastname: Lin
authors.forename: Shaohua
authors.initials: S
authors.lastname: Huang
authors.forename: Zufang
authors.initials: Z
authors.lastname: Huang
authors.forename: Yongzeng
authors.initials: Y
authors.lastname: Li
authors.forename: Juqiang
authors.initials: J
authors.lastname: Lin
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.forename: Haishan
authors.initials: H
authors.lastname: Zeng
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods*; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis; D009369:Neoplasms / Q000648:ultrastructure; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000379:methods*; D000069453:Ultrasonic Waves
chemical_list: D012834:Silver