title: devR PCR for the diagnosis of intraocular tuberculosis.
abstract: OBJECTIVE
  To compare the efficacy of devR and MPB64 PCR in the diagnosis of intraocular tuberculosis.
  
  
  METHODS
  Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of Mycobacterium tuberculosis (MTB) to detect sensitivity and specificity.
  
  
  RESULTS
  devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively.
  
  
  CONCLUSIONS
  devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.
authors.affiliation: Advanced Eye Center, Post Graduate Institute of Medical Education and Research , Chandigarh , India .
authors.forename: Pankaj
authors.initials: P
authors.lastname: Kataria
authors.forename: Abiraj
authors.initials: A
authors.lastname: Kumar
authors.forename: Reema
authors.initials: R
authors.lastname: Bansal
authors.forename: Aman
authors.initials: A
authors.lastname: Sharma
authors.forename: Vishali
authors.initials: V
authors.lastname: Gupta
authors.forename: Amod
authors.initials: A
authors.lastname: Gupta
authors.forename: Ramandeep
authors.initials: R
authors.lastname: Singh
authors.forename: Kusum
authors.initials: K
authors.lastname: Sharma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001082:Aqueous Humor / Q000821:virology; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D014392:Tuberculosis, Ocular / Q000175:diagnosis* / Q000382:microbiology; D014605:Uveitis / Q000175:diagnosis* / Q000382:microbiology; D014822:Vitreous Body / Q000821:virology; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: MPB64 PCR; devR PCR; polymerase chain reaction; retinal vasculitis; tubercular vasculitis