title: Heterosis and combining ability estimates in isoflavone content using different parental soybean accessions: wild soybean, a valuable germplasm for soybean breeding.
abstract: Isoflavone, a group of secondary metabolites in soybean, is beneficial to human health. Improving isoflavone content in soybean seeds has become one of the most important breeding objectives. However, the narrow genetic base of soybean cultivars hampered crop improvement. Wild soybean is an extraordinarily important gene pool for soybean breeding. In order to select an optimal germplasm for breeding programs to increase isoflavone concentration, 36 F1 soybean progenies from different parental accessions (cultivars, wild, Semi-wild and Interspecific) with various total isoflavone (TIF) concentration (High, Middle, Low) were analyzed for their isoflavone content. Results showed that male parents, except for Cultivars, showed positive GCA effects. In particular, wild soybean had higher positive GCA effects for TIF concentration. Both MP and BP heterosis value declined in the hybrid in which male parents were wild soybean, semi-wild soybean, interspecific offspring and cultivar in turn. In general, combining ability and heterosis in hybrids which had relative higher TIF concentration level parents showed better performance than those which had lower TIF concentration level parents. These results indicated characteristics of isoflavone content were mainly governed by additive type of gene action, and wild relatives could be utilized for breeding of soybean cultivars with this trait. A promising combination was found as the best potential hybrid for isoflavone content improvement.
authors.affiliation: Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Northeast Forestry University Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China; Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Yingdong
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Jialei
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Institute of Crops Breeding, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Soybean, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Luan
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Bixian
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Food Processing, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Xie
authors.affiliation: Life Science and Technology College, Harbin Normal University, No.1 South Normal University Road, Limin Economic and Technological Development Zone, Harbin, 150025, China.
authors.forename: Donglin
authors.initials: D
authors.lastname: Guo
authors.affiliation: Institute of Crops Tillage and Cultivation, Heilongjiang Academy of Agricultural Sciences, 368 Xuefu Road, Harbin, 150086, China.
authors.forename: Yongcai
authors.initials: Y
authors.lastname: Lai
mesh_terms: D003433:Crosses, Genetic; D006801:Humans; D006823:Hybrid Vigor / Q000502:physiology*; D000069600:Plant Breeding; D013025:Glycine max / Q000235:genetics*