title: Multiple oral carcinomas associated with a novel papillomavirus in a dog.
abstract: Papillomaviruses (PVs) are well recognized to cause human oral squamous cell carcinomas (SCCs). However, there is currently little evidence that PVs similarly cause oral cancer in dogs. In the present case, a dog developed an invasive SCC and multiple in situ carcinomas within the mouth. Cell changes consistent with PV infection were prominent within the neoplasms and the surrounding gingiva. Immunohistochemical staining revealed PV antigens and intense p16(CDKN2A) protein (p16) immunostaining within the invasive SCC. Papillomaviral DNA sequences were amplified from the invasive and in situ carcinomas. Sequencing revealed that the DNA was from a novel PV that appears most closely related to canine PV-2 and -7. To the authors' knowledge, multiple carcinomas have not been previously reported in the mouth of a dog. Additionally, the current study describes PV cytopathology in a canine oral SCC. Whether the PV infection influenced neoplasm development cannot be definitively determined in this case. However, the presence of p16 immunostaining and the development of multiple oral carcinomas support a role of the PV in tumorigenesis in this dog.
authors.affiliation: Institute of Veterinary, Animal, and Biomedical Sciences, Massey University, Palmerston North, New Zealand (Munday)Auckland Veterinary Dentistry and Oral Surgery, Auckland, New Zealand (Tucker)Department of Pathobiology and Diagnostic Investigation/Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI (Kuipel)New Zealand Veterinary Pathology Ltd, Auckland, New Zealand (Harvey) j.munday@massey.ac.nz.
authors.forename: John S
authors.initials: JS
authors.lastname: Munday
authors.affiliation: Institute of Veterinary, Animal, and Biomedical Sciences, Massey University, Palmerston North, New Zealand (Munday)Auckland Veterinary Dentistry and Oral Surgery, Auckland, New Zealand (Tucker)Department of Pathobiology and Diagnostic Investigation/Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI (Kuipel)New Zealand Veterinary Pathology Ltd, Auckland, New Zealand (Harvey).
authors.forename: Russell S
authors.initials: RS
authors.lastname: Tucker
authors.affiliation: Institute of Veterinary, Animal, and Biomedical Sciences, Massey University, Palmerston North, New Zealand (Munday)Auckland Veterinary Dentistry and Oral Surgery, Auckland, New Zealand (Tucker)Department of Pathobiology and Diagnostic Investigation/Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI (Kuipel)New Zealand Veterinary Pathology Ltd, Auckland, New Zealand (Harvey).
authors.forename: Matti
authors.initials: M
authors.lastname: Kiupel
authors.affiliation: Institute of Veterinary, Animal, and Biomedical Sciences, Massey University, Palmerston North, New Zealand (Munday)Auckland Veterinary Dentistry and Oral Surgery, Auckland, New Zealand (Tucker)Department of Pathobiology and Diagnostic Investigation/Diagnostic Center for Population and Animal Health, Michigan State University, East Lansing, MI (Kuipel)New Zealand Veterinary Pathology Ltd, Auckland, New Zealand (Harvey).
authors.forename: Catherine J
authors.initials: CJ
authors.lastname: Harvey
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000662:veterinary*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D007150:Immunohistochemistry / Q000662:veterinary; D008297:Male; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000662:veterinary*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004279:DNA, Viral
keywords: Canine; carcinogenesis; dogs; neoplasia; oral; papillomavirus