title: GPR39 marks specific cells within the sebaceous gland and contributes to skin wound healing.
abstract: G protein-coupled receptors (GPCRs) mediate multiple key biological processes in the body. The orphan receptor GPR39 has been reported to be involved in various pathophysiological events. However, the function of GPR39 in skin biology remains unknown. Using a genetically engineered mouse strain in which lacZ expression faithfully replaced endogenous Gpr39 expression, we discovered a unique expression pattern of Gpr39 in the sebaceous gland (SG). Using various methods, we confirmed that GPR39 marked a specific cell population at the opening of the SG and colocalised with the SG stem cell marker Blimp1. Further investigations showed that GPR39 was spatiotemporally expressed during skin wound repair. Although it was dispensable for skin development and homeostasis, GPR39 contributed positively to skin wound healing: its loss led to a delay in wound healing during the intermediate stage. The present study reveals a novel role of GPR39 in both dermatology and stem cell biology that has not been previously recognised.
authors.affiliation: 1] State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China [2] University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Huashan
authors.initials: H
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, China.
authors.forename: Jingqiao
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Department of Histology&Embryology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
authors.forename: Shoubing
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Huishan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1] State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China [2] University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Ning
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Animal Science and Technology, Beijing University of Agriculture, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Enkui
authors.initials: E
authors.lastname: Duan
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D000074462:Positive Regulatory Domain I-Binding Factor 1; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*; D012627:Sebaceous Glands / Q000378:metabolism* / Q000473:pathology; D013234:Stem Cells / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014945:Wound Healing
chemical_list: D000943:Antigens, Differentiation; C512928:GPR39 protein, mouse; C513258:Prdm1 protein, mouse; D043562:Receptors, G-Protein-Coupled; D014157:Transcription Factors; D000074462:Positive Regulatory Domain I-Binding Factor 1