title: Neuroglobin expression in rats after traumatic brain injury.
abstract: In this study, we used a rat model of severe closed traumatic brain injury to explore the relationship between neuroglobin, brain injury and neuronal apoptosis. Real-time PCR showed that neuroglobin mRNA expression rapidly increased in the rat cerebral cortex, and peaked at 30 minutes and 48 hours following traumatic brain injury. Immunohistochemical staining demonstrated that neuroglobin expression increased and remained high 2 hours to 5 days following injury. The rate of increase in the apoptosis-related Bax/Bcl-2 ratio greatly decreased between 30 minutes and 1 hour as well as between 48 and 72 hours post injury. Expression of neuroglobin and the anti-apoptotic factor Bcl-2 greatly increased, while that of the proapoptotic factor decreased, in the cerebral cortex post severe closed traumatic brain injury. It suggests that neuroglobin might protect neurons from apoptosis after traumatic injury by regulating Bax/Bcl-2 pathway.
authors.affiliation: Department of Surgery, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Traumatic-Aesthetic Surgery, Huangsi Aesthetic Surgery Hospital, Beijing 100120, China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Division of Surgery, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Aijia
authors.initials: A
authors.lastname: Shang
authors.affiliation: Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Yazhuo
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Cardiology, Division of Nanlou, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Suyan
authors.initials: S
authors.lastname: Bian
authors.affiliation: Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
authors.forename: Zhongfeng
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Division of Surgery, General Hospital of Chinese PLA, Beijing 100853, China.
authors.forename: Dingbiao
authors.initials: D
authors.lastname: Zhou
keywords: Bax; Bcl-2; apoptosis; cerebral cortex; neural regeneration; neuroglobin; neuron; traumatic brain injury