title: Sequence-specific label-free nucleic acid biosensor for the detection of the hepatitis C virus genotype 1a using a disposable pencil graphite electrode.
abstract: In this paper, we demonstrate a simple, sensitive, inexpensive, disposable and label-free electrochemical nucleic acid biosensor for the detection of the hepatitis C virus genotype 1a (HCV1a). The nucleic acid biosensor was designed with the amino-linked inosine-substituted 20-mer probes, which were immobilized onto a disposable pencil graphite electrode (PGE) by covalent linking. The proposed nucleic acid biosensor was linear in the range of 0.05 and 0.75 μM, exhibiting a limit of detection of 54.9 nM. The single-stranded synthetic PCR product analogs of HCV1a were also detected with satisfactory results under optimal conditions, showing the potential application of this biosensor.
authors.affiliation: a Department of Chemistry , Faculty of Science and Art, Nevsehir Haci Bektas Veli University , Nevsehir , Turkey.
authors.forename: Soner
authors.initials: S
authors.lastname: Donmez
authors.affiliation: b Department of Chemistry , Faculty of Science, Gazi University , Ankara , Turkey.
authors.forename: Fatma
authors.initials: F
authors.lastname: Arslan
authors.affiliation: b Department of Chemistry , Faculty of Science, Gazi University , Ankara , Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Arslan
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D015342:DNA Probes / Q000737:chemistry*; D004566:Electrodes; D005838:Genotype; D060005:Genotyping Techniques / Q000295:instrumentation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D016174:Hepacivirus / Q000737:chemistry / Q000235:genetics*
chemical_list: D015342:DNA Probes; D006108:Graphite
keywords: hepatitis C virus; inosine; nucleic acid biosensor; pencil graphite electrode; square wave voltammetry