title: miR-196a expression in human and canine osteosarcomas: a comparative study.
abstract: Osteosarcoma (OS) is the most common primary malignant bone tumour in dogs and humans. MicroRNAs are short non-coding RNA molecules involved in post-transcriptional gene expression. Here, we compared the effects of miR-196a deregulation in human and canine OS cells after having observed a more uniform distribution and stronger down-expression in the human specimens. Cell response to miR-196a transfection was different in human and canine OS. A decreased proliferation rate was seen in human MG63 and 143B OS cells, while no appreciable changes occurred in canine DAN cells. Transient decrease of motility was highly remarkable and longer in MG63, concomitant with decreased levels of annexin1, a target of miR-196a promoting cell migration and invasion. In conclusion, the effects of miR-196a over-expression on tumour cell response may be strictly related to species and cell type. Further studies are needed to define the impact of miRNA deregulation on OS development.
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy. Electronic address: laura.pazzaglia@ior.it.
authors.forename: Laura
authors.initials: L
authors.lastname: Pazzaglia
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Leonardi
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Amalia
authors.initials: A
authors.lastname: Conti
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Novello
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Irene
authors.initials: I
authors.lastname: Quattrini
authors.affiliation: Department of Paediatrics, University Hospital of Parma, Via Gramsci 14, 43126 Parma, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Montanini
authors.affiliation: Department of Biology, University "Federico II" of Napoli, Via Delpino 1, 80126 Napoli, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Roperto
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine - Louisiana State University, 1909 Skip Bertman Drive, Baton Rouge, LA 70803, USA.
authors.forename: Fabio
authors.initials: F
authors.lastname: Del Piero
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Viale Crucioli, 122, 64100 Teramo, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Di Guardo
authors.affiliation: Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126 Perugia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Piro
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Piero
authors.initials: P
authors.lastname: Picci
authors.affiliation: Laboratory of Experimental Oncology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136 Bologna, Italy.
authors.forename: Maria Serena
authors.initials: MS
authors.lastname: Benassi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D004283:Dog Diseases / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D014162:Transfection
chemical_list: C542543:MIRN196 microRNA, human; D035683:MicroRNAs
keywords: Migration; Osteosarcoma specimens; Real-Time PCR; Target gene; miR-196a transfection