title: Demonstration of the biphasic release of 0.1% halcinonide cream.
abstract: Halcinonide in Halog Cream is formulated in a unique cream base that allows a biphasic release of this Class II steroid. Halcinonide in this product exists in two phases--a solution phase that is released immediately and a suspension phase that has a delayed release over time. The goal of this study was to evaluate this biphasic release of halcinonide into the skin using a novel noninvasive method. A dermal tape stripping protocol was used to quantify halcinonide concentration at 6 sequential depths in the skin of 4 sites on the forearms of 5 subjects. D-Squame strips were sequentially applied with consistent pressure and removed at 1, 3, 6, and 9 hours after application of halcinonide. Halcinonide was extracted from the strips and quantified using liquid chromatography mass spectrometry. The highest concentration of halcinonide was detected in strip 1 from the superficial stratum corneum with subsequent reduction in deeper stratum corneum layers as expected. The concentration increased beyond the first hour after application and demonstrated a sustained release into hour 6 before beginning declining. Similarly, the average concentration of halcinonide in strips 1 to 6 peaked at hour 1 and remained elevated for 6 hours. Data collected from the tape strips demonstrated a biphasic release of halcinonide--immediate release within 1 hour of application from the solution phase and continued, sustained release from the suspension phase following the first hour of application.
authors.forename: Zoe Diana
authors.initials: ZD
authors.lastname: Draelos
mesh_terms: D000279:Administration, Cutaneous; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid; D003692:Delayed-Action Preparations; D065546:Drug Liberation; D006206:Halcinonide / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006801:Humans; D013058:Mass Spectrometry; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption; D063465:Skin Cream; D053832:Surgical Tape; D013997:Time Factors
chemical_list: D000893:Anti-Inflammatory Agents; D003692:Delayed-Action Preparations; D006206:Halcinonide