title: Interaction of a patterned amphiphilic polyphenylene dendrimer with a lipid monolayer: electrostatic interactions dominate.
abstract: Dendrimeric macromolecules with defined shape and size are promising candidates for delivering drug or DNA molecules into cells. In this work we study the influence of an amphiphilic polyphenylene dendrimer on a model cell membrane consisting of a condensed 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayer. A small surface pressure decrease is observed when the dendrimer solution is injected into the aqueous phase below the monolayer. X-ray reflectivity measurements show that the surface monolayer remains intact. The molecular-scale picture is obtained with sum-frequency generation spectroscopy. With this technique, we observe that the tails of the surfactant molecules become less ordered upon interaction with the amphiphilic polyphenylene dendrimer. In contrast, the water molecules below the DPPC layer become more ordered. Our observations suggest that electrostatic interactions between the negative charge of the dendrimer and the positively charged part of the DPPC headgroup keep the dendrimer located below the headgroup. No evidence of dendrimer insertion into the membrane has been observed. Apparently before entering the cell membrane the dendrimer can stick at the hydrophilic part of the lipids.
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Masanari
authors.initials: M
authors.lastname: Okuno
authors.forename: Markus
authors.initials: M
authors.lastname: Mezger
authors.forename: René
authors.initials: R
authors.lastname: Stangenberg
authors.forename: Martin
authors.initials: M
authors.lastname: Baumgarten
authors.forename: Klaus
authors.initials: K
authors.lastname: Müllen
authors.forename: Mischa
authors.initials: M
authors.lastname: Bonn
authors.forename: Ellen H G
authors.initials: EH
authors.lastname: Backus
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry*; D055672:Static Electricity; D013500:Surface Tension
chemical_list: D050091:Dendrimers; D011108:Polymers; D015060:1,2-Dipalmitoylphosphatidylcholine; C041325:polyphenylene sulfide