title: An amplified electrochemical aptasensor based on hybridization chain reactions and catalysis of silver nanoclusters.
abstract: In the present study, based on the mimic oxidase catalytic character of nucleic-acid-stabilized silver nanoclusters (DNA/AgNCs) and hybridization chain reactions for signal amplification, the fabrication of a label-free sensitive "turn-on" electrochemical aptasensor for the amplified determination of lysozyme was demonstrated. First, the designed DNA duplex was modified on the electrode. With the specific binding of the target, lysozyme and its aptamer, the lysozyme-binding DNA sequence was liberated, exposing the induced DNA sequence, which in turn triggered the formation of the supersandwich DNA structure. Because the cytosine-rich sequence was designed ingeniously on the DNA sequence, DNA/AgNCs were formed on the supersandwich DNA structure. The peroxidase-like character of DNA/AgNCs produced detectable electrochemical signals for the lysozyme aptasensor, which showed a satisfying sensitive detection of lysozyme with a low detection limit of 42 pM and a wide linear range of 10(-10) M to 10(-5) M.
authors.affiliation: Key Laboratory of Chem-Biosensing, Anhui province; Key Laboratory of Functional Molecular Solids, Anhui province; College of Chemistry and Materials Science, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China. xjzhang@mail.ahnu.edu.cn wangyuz@mail.ahnu.edu.cn.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Sha
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Qiu
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques; D002138:Calibration; D002384:Catalysis; D004247:DNA / Q000737:chemistry; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D012834:Silver / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D001672:Biocompatible Materials; D009841:Oligonucleotides; D012834:Silver; D006046:Gold; D004247:DNA; D006861:Hydrogen Peroxide; D009113:Muramidase