title: Protein immobilization onto electrochemically synthesized CoFe nanowires.
abstract: CoFe nanowires have been synthesized by the electrodeposition technique into the pores of a polycarbonate membrane with a nominal pore diameter of 50 nm, and the composition of CoFe nanowires varying by changing the source concentration of iron. The synthesized nanowire surfaces were functionalized with amine groups by treatment with aminopropyltriethoxysilane (APTES) linker, and then conjugated with streptavidin-Cy3 protein via ethyl (dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide coupling chemistry. The oxide surface of CoFe nanowire is easily modified with aminopropyltriethoxysilane to form an amine terminating group, which is covalently bonded to streptavidin-Cy3 protein. The physicochemical properties of the nanowires were analyzed through different characterization techniques such as scanning electron microscope, energy dispersive spectroscopy, and vibrating sample magnetometer. Fluorescence microscopic studies and Fourier transform infrared studies confirmed the immobilization of protein on the nanowire surface. In addition, the transmission electron microscope analysis reveals the thin protein layer which is around 12-15 nm on the nanowire surfaces.
authors.affiliation: Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea.
authors.forename: Sri Ramulu
authors.initials: SR
authors.lastname: Torati
authors.affiliation: Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea.
authors.forename: Venu
authors.initials: V
authors.lastname: Reddy
authors.affiliation: Department of Physics, Andong National University, Andong, South Korea.
authors.forename: Seok Soo
authors.initials: SS
authors.lastname: Yoon
authors.affiliation: Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea.
authors.forename: CheolGi
authors.initials: C
authors.lastname: Kim
mesh_terms: D002232:Carbocyanines; D003035:Cobalt / Q000737:chemistry*; D004595:Electroplating / Q000379:methods*; D005296:Ferrous Compounds / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry*; D053770:Nanowires / Q000737:chemistry*; D019809:Streptavidin
chemical_list: D002232:Carbocyanines; D005296:Ferrous Compounds; D055767:Immobilized Proteins; C089187:cyanine dye 3; C007717:ferrocobalt; D003035:Cobalt; D019809:Streptavidin
keywords: biofunctionalization; electrodeposition; streptavidin