title: Ultrasonic and free-radical degradation of mannan from Candida albicans.
abstract: Mannan from pathogenic Candida albicans serotype A was degraded by means of ultrasound and/or OH generated in situ by Fenton reaction. The kinetics of degradation was monitored by HPLC analysis and the weight-average molecular weights (Mw) and index of polydispersity (PDI) were compared. A well-defined low-molecular-weight mannan (∼ 30 kDa) with narrowed PDI of 1.8 was obtained after 120 min of ultrasonication. Similar or even lower Mw (up to 16 kDa) was achieved upon free-radical exposure depending on Fe(2+) concentration used; however, this was accompanied by overall broadening of PDI and distinct changes in polymer structure as indicated by NMR analysis.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: alzbeta.cizova@savba.sk.
authors.forename: Alžbeta
authors.initials: A
authors.lastname: Čížová
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Bystrický
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic.
authors.forename: Slavomír
authors.initials: S
authors.lastname: Bystrický
mesh_terms: D002176:Candida albicans / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry*; D007700:Kinetics; D008351:Mannans / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D005609:Free Radicals; D008351:Mannans
keywords: Candida albicans; Degradation; Fenton reaction; Mannan; Ultrasound