title: Accuracy of the Thermo Fisher Scientific (Sensititre™) dry-form broth microdilution MIC product when testing ceftaroline.
abstract: Ceftaroline, the active metabolite of the ceftaroline fosamil pro-drug, was the first advanced-spectrum cephalosporin with potent activity against methicillin-resistant Staphylococcus aureus to be approved by the US Food and Drug Administration for acute bacterial skin and skin structure infections. After 4 years of clinical use, few ceftaroline commercial susceptibility testing devices other than agar diffusion methods (disks and stable gradient) are available. Here, we validate a broth microdilution product (Sensititre™; Thermo Fisher Scientific, Cleveland, OH, USA) that achieved 99.2% essential agreement (manual and automated reading) and 95.3-100.0% categorical agreement, with high reproducibility (98.0-100.0%). Sensititre™ MIC values for ceftaroline, however, were slightly skewed toward an elevated value (0.5 × log2 dilution step), greatest when testing for streptococci and Enterobacteriaceae.
authors.affiliation: JMI Laboratories, North Liberty, IA, USA. Electronic address: ronald-jones@jmilabs.com.
authors.forename: Ronald N
authors.initials: RN
authors.lastname: Jones
authors.affiliation: ThermoFisher Scientific, Cleveland, OH, USA.
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Holliday
authors.affiliation: Cerexa, Inc, Oakland, CA, USA.
authors.forename: Ian A
authors.initials: IA
authors.lastname: Critchley
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002511:Cephalosporins / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D014481:United States; D000097583:Ceftaroline
chemical_list: D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D000097583:Ceftaroline
keywords: Ceftaroline; Sensititre®; Susceptibility testing; Validation