title: 5-Sulfosalicylic acid dihydrate-based pretreatment for the modification of enzyme-linked immunoassay of fluoroquinolones in fishery products.
abstract: A simple, rapid sample extraction method for the determination of FQs was developed. Fishery samples were extracted with 2% of 5-sulfosalicylic acid dihydrate and the extracts were analyzed directly without any further purification or clean-up procedures. The FQs were determined with standards of 2% of 5-sulfosalicylic acid dihydrate in the concentration range of 0.1-25.6 μg L(-1), and the limit of detection (LOD) was 0.1 μg L(-1). The matrix interference originated from fishery samples was eliminated by 2% of 5-sulfosalicylic acid dihydrate and did not interact with horseradish peroxidase (HRP) labeled IgG in western blotting. No significant matrix interference was observed as samples extracted with 2% of 5-sulfosalicylic acid dihydrate. Recoveries of FQs in fishery muscle were between 72.37-94.35% in the concentrations range of 10-50 μg kg(-1).This extraction procedure was much rapider and simpler to conventional ELISA extraction procedure and could be used as a time-saving and cost-effective method for FQs monitoring in fishery samples.
authors.affiliation: a Food Safety Laboratory , Ocean University of China , Qingdao , China.
authors.forename: Mengqi
authors.initials: M
authors.lastname: Cui
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.forename: Xiudan
authors.initials: X
authors.lastname: Wang
authors.forename: Limin
authors.initials: L
authors.lastname: Cao
authors.forename: Jianxin
authors.initials: J
authors.lastname: Sui
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005398:Fisheries; D024841:Fluoroquinolones / Q000032:analysis*; D057230:Limit of Detection; D009132:Muscles / Q000737:chemistry; D012459:Salicylates / Q000737:chemistry*
chemical_list: D001557:Benzenesulfonates; D024841:Fluoroquinolones; D012459:Salicylates; C003366:sulfosalicylic acid
keywords: 5-sulfosalicylic acid dihydrate; ci-ELISA; extraction; fluoroquinolones