title: Discovery of a new family of Dieckmann cyclases essential to tetramic acid and pyridone-based natural products biosynthesis.
abstract: Bioinformatic analyses indicate that TrdC, SlgL, LipX2, KirHI, and FacHI belong to a group of highly homologous proteins involved in biosynthesis of actinomycete-derived tirandamycin B, streptolydigin, α-lipomycin, kirromycin, and factumycin, respectively. However, assignment of their biosynthetic roles has remained elusive. Gene inactivation and complementation, in vitro biochemical assays with synthetic analogues, point mutations, and phylogenetic tree analyses reveal that these proteins represent a new family of Dieckmann cyclases that drive tetramic acid and pyridone scaffold biosynthesis.
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences , 164 West Xingang Road, Guangzhou 510301, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Gui
authors.forename: Qinglian
authors.initials: Q
authors.lastname: Li
authors.forename: Xuhua
authors.initials: X
authors.lastname: Mo
authors.forename: Xiangjing
authors.initials: X
authors.lastname: Qin
authors.forename: Junying
authors.initials: J
authors.lastname: Ma
authors.forename: Jianhua
authors.initials: J
authors.lastname: Ju
mesh_terms: D000190:Actinomyces / Q000201:enzymology*; D000617:Aminoglycosides / Q000378:metabolism; D001688:Biological Products / Q000737:chemistry* / Q000378:metabolism; D003500:Cyclization; D005798:Genes, Bacterial / Q000502:physiology; D006027:Glycosides / Q000378:metabolism; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D019761:Phosphorus-Oxygen Lyases / Q000378:metabolism*; D010802:Phylogeny; D011090:Polyenes / Q000378:metabolism; D011728:Pyridones / Q000378:metabolism; D011760:Pyrrolidinones / Q000737:chemistry*
chemical_list: D000617:Aminoglycosides; D001688:Biological Products; D006027:Glycosides; D011090:Polyenes; D011728:Pyridones; D011760:Pyrrolidinones; C548829:alpha-lipomycin; C009435:tetramic acid; C021492:tirandamycin B; C100307:streptolydigin; C037260:factumycin; D019761:Phosphorus-Oxygen Lyases; C008536:mocimycin