title: Aβ induces oxidative stress in senescence-accelerated (SAMP8) mice.
abstract: According to the amyloid hypothesis, amyloid β accumulates in brains with Alzheimer's disease (AD) and triggers cell death and memory deficit. Previously, we developed a rice Aβ vaccine expressing Aβ, which reduced brain Aβ levels in the Tg2576 mouse model of familial AD. We used senescence-accelerated SAMP8 mice as a model of sporadic AD and investigated the relationship between Aβ and oxidative stress. Insoluble Aβ and 4-hydroxynonenal (4-HNE) levels tended to be reduced in SAMP8 mice-fed the rice Aβ vaccine. We attempted to clarify the relationship between oxidative stress and Aβ in vitro. Addition of Aβ peptide to the culture medium resulted in an increase in 4-HNE levels in SH-SY5Y cells. Tg2576 mice, which express large amounts of Aβ in their brain, also exhibited increased 4-HNE levels; this increase was inhibited by the Aβ vaccine. These results indicate that Aβ induces oxidative stress in cultured cells and in the mouse brain.
authors.affiliation: a Department of Biological Sciences, Graduate School of Science , The University of Tokyo , Tokyo , Japan.
authors.forename: Kurara
authors.initials: K
authors.lastname: Takagane
authors.forename: Jun
authors.initials: J
authors.lastname: Nojima
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Mitsuhashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suo
authors.forename: Dai
authors.initials: D
authors.lastname: Yanagihara
authors.forename: Fumio
authors.initials: F
authors.lastname: Takaiwa
authors.forename: Yasuomi
authors.initials: Y
authors.lastname: Urano
authors.forename: Noriko
authors.initials: N
authors.lastname: Noguchi
authors.forename: Shoichi
authors.initials: S
authors.lastname: Ishiura
mesh_terms: D000375:Aging; D000447:Aldehydes / Q000378:metabolism; D000544:Alzheimer Disease / Q000378:metabolism* / Q000503:physiopathology / Q000517:prevention & control; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002021:Buffers; D006801:Humans; D008297:Male; D018782:Maze Learning; D051379:Mice; D008822:Mice, Transgenic; D012275:Oryza / Q000235:genetics; D018384:Oxidative Stress; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D014612:Vaccines / Q000235:genetics
chemical_list: D000447:Aldehydes; D016229:Amyloid beta-Peptides; D002021:Buffers; D010446:Peptide Fragments; D014612:Vaccines; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42); C027576:4-hydroxy-2-nonenal
keywords: 4-hydroxynonenal; Alzheimer’s disease; amyloid; edible vaccine; oxidative stress