title: Role of adenovirus-mediated retinoblastoma 94 in the treatment of human non-small cell lung cancer.
abstract: Non‑small cell lung cancer (NSCLC) remains the leading cause of cancer‑related mortality despite the fact that great advances have been made in therapeutic treatment methods. Therefore, in the present study, the role of adenovirus-mediated retinoblastoma 94 (Ad‑RB94) gene therapy in NSCLC was investigated. Following treatment with Ad‑RB94, the proportion of A549 cells in the G2/M phase was increased. In the mouse xenograft model, the overexpression of RB94 inhibited the tumor growth compared with the control group and the Ad-‑LacZ-treated group. In the transplanted tumors, the overexpression of RB94 induced the apoptosis of tumors as well as an increase in the mRNA levels of cyclinB1. In conclusion, the results of the present study suggested that RB94 may effectively inhibit NSCLC tumor cell growth by inducing G2/M cell cycle arrest and apoptosis, indicating that RB94 may be a promising candidate for adjuvant therapy with radiation or chemotherapy in NSCLC.
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
authors.forename: Qingbao
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Oncology, The People's Hospital of Binzhou City, Binzhou, Shandong 256610, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Ultrasonography, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250001, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D056744:Cyclin B1 / Q000235:genetics; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D016160:Retinoblastoma Protein / Q000737:chemistry / Q000235:genetics*; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
chemical_list: D056744:Cyclin B1; D012333:RNA, Messenger; D016160:Retinoblastoma Protein