title: Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose.
abstract: In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.
authors.affiliation: Embrapa Cerrados, BR 020, Km 18, CEP 73310-970, Brasília, Distrito Federal, Brazil. Electronic address: carolgonzaless@gmail.com.
authors.forename: C G
authors.initials: CG
authors.lastname: Silva
authors.affiliation: Embrapa Cerrados, BR 020, Km 18, CEP 73310-970, Brasília, Distrito Federal, Brazil. Electronic address: ercunha@gmail.com.
authors.forename: E R
authors.initials: ER
authors.lastname: Cunha
authors.affiliation: Embrapa Cerrados, BR 020, Km 18, CEP 73310-970, Brasília, Distrito Federal, Brazil. Electronic address: gui_blume@hotmail.com.
authors.forename: G R
authors.initials: GR
authors.lastname: Blume
authors.affiliation: Embrapa Cerrados, BR 020, Km 18, CEP 73310-970, Brasília, Distrito Federal, Brazil. Electronic address: juaci.malaquias@embrapa.br.
authors.forename: J V
authors.initials: JV
authors.lastname: Malaquias
authors.affiliation: Universidade de Brasília, Departamento de Biologia Celular, Instituto de Ciências Biológicas, CEP 70919-970, Brasília, Distrito Federal, Brazil. Electronic address: snbao@unb.br.
authors.forename: S N
authors.initials: SN
authors.lastname: Báo
authors.affiliation: Embrapa Cerrados, BR 020, Km 18, CEP 73310-970, Brasília, Distrito Federal, Brazil. Electronic address: carlos.martins@embrapa.br.
authors.forename: C F
authors.initials: CF
authors.lastname: Martins
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D003059:Cocos / Q000737:chemistry*; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004126:Dimethylformamide / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D006801:Humans; D007785:Lactose / Q000737:chemistry; D008297:Male; D012661:Semen / Q000502:physiology; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology; D034421:Sus scrofa; D014199:Trehalose / Q000737:chemistry
chemical_list: D003451:Cryoprotective Agents; D004126:Dimethylformamide; D014199:Trehalose; D007785:Lactose; D005990:Glycerol
keywords: Artificial insemination; Conservation; Cryopreservation; Spermatozoa; Swine