title: Alantolactone Induces Apoptosis and Cell Cycle Arrest on Lung Squamous Cancer SK-MES-1 Cells.
abstract: Alantolactone, a sesquiterpene lactone compound, has variety of pharmacological properties, including anti-inflammatory and antineoplastic effects. In our study, alantolactone inhibited cancer cell proliferation. To explore the mechanisms underlying its antitumor action, we further examined apoptotic cells and cell cycle distribution using flow cytometry analysis. Alantolactone triggered apoptosis and induced cell cycle G1/G0 phase arrest. Furthermore, the expressions of caspases-8, -9, -3, PARP, and Bax were significantly upregulated, while antiapoptotic factor Bcl-2 expression was inhibited. In addition, the expressions of cyclin-dependent kinase 4 (CDK4), CDK6, cyclin D3, and cyclin D1 were downregulated by alantolactone. Therefore, our findings indicated that alantolactone has an antiproliferative role on lung squamous cancer cells, and it may be a promising chemotherapeutic agent for squamous lung cancer SK-MES-1 cells.
authors.affiliation: Department of Anesthesiology, The Second Hospital of Jilin University, Changchun, Jilin, 130041, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Zhenxiang
authors.initials: Z
authors.lastname: Pan
authors.forename: Yungang
authors.initials: Y
authors.lastname: Luo
authors.forename: Leilei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Guangxin
authors.initials: G
authors.lastname: Zhang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ranji
authors.initials: R
authors.lastname: Cui
authors.forename: Mei
authors.initials: M
authors.lastname: Sun
authors.forename: Xingyi
authors.initials: X
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007783:Lactones / Q000008:administration & dosage*; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D045787:Sesquiterpenes, Eudesmane / Q000008:administration & dosage*
chemical_list: D007783:Lactones; D009363:Neoplasm Proteins; D045787:Sesquiterpenes, Eudesmane; C004363:alantolactone
keywords: Alantolactone; Apoptosis; Cell Cycle; SK-MES-1 Cell