title: [Application of polyclonal break-apart probes in the diagnosis of Xp11.2 translocation renal cell carcinoma].
abstract: OBJECTIVE
  To explore the value of self-designed fluorescent in situ hybridization (FISH) polyclonal break-apart probes specific for TFE3 gene in the diagnosis of Xp11.2 translocation renal cell carcinoma.
  
  
  METHODS
  All tissue samples were collected from 2006 to 2013, including Xp11.2 translocation renal cell carcinoma (n = 10), renal clear cell carcinoma (n = 10) and renal papillary cell carcinoma (n = 10). FISH was conducted for paraffin-embedded tumor tissue sections with probes. The types of fluorescence were observed by fluorescent microscopy to determine the existence or non-existence of translocated TFE3 gene.
  
  
  RESULTS
  All sections were successfully probed. The split red and green signals within a single nucleus were detected simultaneously in 9 cases of Xp11.2 translocation renal cell carcinoma as diagnosed by traditional pathological and immunohistochemical methods. And it was consistent with the initial diagnosis. Detection of fusion signal in 1/10 and negative FISH result did not conform to the initial diagnosis. The fluorescent types of renal clear cell carcinoma and renal papillary cell carcinoma were all fusion signals. FISH tests were negative for renal clear and papillary cell carcinomas.
  
  
  CONCLUSIONS
  Xp11.2 translocation renal cell carcinomas diagnosed by traditional pathological and immunohistochemical methods are sometimes misdiagnosed. Detecting the translocation of TFE3 gene with FISH polyclonal break-apart probes is both accurate and reliable for diagnosing Xp11.2 translocation renal cell carcinoma.
authors.affiliation: Department of Urology, Affiliated Drum Tower Hospital, College of Medicine, Nanjing University, Nanjing 210008, China.
authors.forename: Xiancheng
authors.initials: X
authors.lastname: Chen
authors.affiliation: Email: gwd@nju.edu.cn.
authors.forename: Weidong
authors.initials: W
authors.lastname: Gan
authors.forename: Qing
authors.initials: Q
authors.lastname: Ye
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Hongqian
authors.initials: H
authors.lastname: Guo
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
mesh_terms: D002292:Carcinoma, Renal Cell; D041321:Chromosomes, Human, X; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007680:Kidney Neoplasms; D014178:Translocation, Genetic