title: [Distribution of human papillomavirus genotypes in early-stadge cervical adenocarcinoma and its clinical significance].
abstract: OBJECTIVE
  To explore the distribution of high-risk HPV-genotypes in early-stage cervical adenocarcinoma and understand the clinical significance of HPV genotyping.
  
  
  METHODS
  From June 2000 to May 2010, a total of 101 paraffin surgical specimens of cervical adenocarcinoma were genotyped by nested polymerase chain reaction (nested PCR). The associations of HPV18 with clinicopathological parameters and survival were further analyzed.
  
  
  RESULTS
  DNA extraction was successfully performed for 96 samples. The HPV-positive rate of early-stage cervical adenocarcinoma was 95.8% (92/96). Two common HPV types were HPV16 (59.4%) and HPV18 (60.4%). The prevalence rates of single, double and multiple HPV infections were 22.9%, 32.3% and 40.6% respectively. The positive rates of lymph node metastasis and vascular involvement with HPV18 infection were 27.6% and 22.4% versus 7.9% and 7.9% for those without HPV18 infection.Univariate analysis showed that positive surgical margin, uterine corpus invasion, lymph node metastasis and HPV18 infection were the predictive factors for poor prognosis of patients with cervical adenocarcinoma. Multivariate analysis revealed that lymph node metastasis was an independent prognostic factor for both disease-free and overall survivals. And uterine corpus invasion was an independent prognostic factor of overall survival for early-stage cervical adenocarcinoma patients.
  
  
  CONCLUSIONS
  HPV16 and HPV18 are major types responsible for early-stage cervical adenocarcinoma.Infection with HPV18 is prone to lymph node metastasis and vascular involvement.However, there is no correlation with disease-free survival or overall survival.
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Junfen
authors.initials: J
authors.lastname: Xu
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Xie
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. Email: lwg@hzcnc.com.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Lü
mesh_terms: D000230:Adenocarcinoma; D004279:DNA, Viral; D018572:Disease-Free Survival; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16; D052161:Human papillomavirus 18; D006801:Humans; D008207:Lymphatic Metastasis; D009367:Neoplasm Staging; D030361:Papillomavirus Infections; D016133:Polymerase Chain Reaction; D015995:Prevalence; D002583:Uterine Cervical Neoplasms
chemical_list: D004279:DNA, Viral