title: Structural elucidation, in vitro antioxidant and photoprotective capacities of a purified polyphenolic-enriched fraction from a saltmarsh plant.
abstract: In temperate saltmarshes, halophytic plants have to daily protect their internal tissues against sunlight and UV rays. Consequently, they develop adaptive responses such as the synthesis of secondary metabolites, including polyphenols. The present study focused on the biological activities of fractions enriched in polyphenols from Salicornia ramosissima. Three different extracts were obtained by purification processes to concentrate polyphenols: a crude hydroalcoholic extract, and two purified fractions: an ethyl acetate fraction (EAF) and an aqueous fraction. Phenolic and flavonoid contents, antioxidant (DPPH radical-scavenging activity, reducing activity, β-carotene linoleic acid system and the ORAC method) and sunscreen properties (Sun Protection Factor and UVA-Protection Factor) were assessed by in vitro tests. The purification process was effective in increasing phenolic and flavonoid contents as well as antioxidant and sunscreen capacities of the EAF. The EAF appeared to be a broad spectrum UV absorber. The chemical structure of 10 EAF polyphenols was elucidated using 2D NMR and mass spectrometry spectra. Furthermore, a correlation was observed between phenolic composition and biological activity. These findings are encouraging for the future use of S. ramosissima as a potential source of antioxidant and photoprotectant molecules for industrial applications.
authors.affiliation: LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France. Electronic address: gwladys.lediouron@univ-brest.fr.
authors.forename: Gwladys
authors.initials: G
authors.lastname: Surget
authors.affiliation: LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Stiger-Pouvreau
authors.affiliation: LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Klervi
authors.initials: K
authors.lastname: Le Lann
authors.affiliation: RMN-RPE-MS, Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), 6 avenue, Victor-Le-Gorgeu-CS93837, 29238 Brest Cedex 3, France.
authors.forename: Nelly
authors.initials: N
authors.lastname: Kervarec
authors.affiliation: Faculty of Pharmacy, Université de Nantes, Nantes Atlantique Universités, LPiC, MMS, EA2160, 1 rue G. Veil BP 53508, 44 000 Nantes, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Couteau
authors.affiliation: Faculty of Pharmacy, Université de Nantes, Nantes Atlantique Universités, LPiC, MMS, EA2160, 1 rue G. Veil BP 53508, 44 000 Nantes, France.
authors.forename: Laurence J M
authors.initials: LJ
authors.lastname: Coiffard
authors.affiliation: Plateforme de spectrométrie de masse FR 2424, CNRS UPMC INSU Station Biologique de Roscoff, Place Georges Teissier, BP 74, F-29682 Roscoff Cedex, France.
authors.forename: Fanny
authors.initials: F
authors.lastname: Gaillard
authors.affiliation: Plateforme de spectrométrie de masse FR 2424, CNRS UPMC INSU Station Biologique de Roscoff, Place Georges Teissier, BP 74, F-29682 Roscoff Cedex, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Cahier
authors.affiliation: LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Guérard
authors.affiliation: LEMAR UMR 6539 UBO CNRS Ifremer IRD, European Institute of Marine Studies (IUEM), Université de Bretagne Occidentale (UBO), European University of Brittany (UEB), Technopôle Brest-Iroise, 29280 Plouzané, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Poupart
mesh_terms: D000085:Acetates / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D019663:Chenopodiaceae / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000085:Acetates; D000975:Antioxidants; D059808:Polyphenols; D011837:Radiation-Protective Agents; C007650:ethyl acetate