title: [Primary fabrication of a tissue-engineered mesh for pelvic floor reconstruction and research in vivo].
abstract: OBJECTIVE
  To explore the fabrication of a tissue-engineered mesh fabricated with autologous adipose-derived mesenchymal stem cells (ADSC) and silk fibroin scaffold for female pelvic reconstruction.
  
  
  METHODS
  Sprague-Dawley rat adipose tissue was obtained from inguinal region. Mesenchymal stem cells were isolated and proliferated in vitro. The sericin of silk was removed after knitting in to web. Then a compound scaffold freeze-dried of silk fibroin web and silk fibroin solution was prepared for ADSC seeding. after 1-week culturing, the cellular morphology and extracellular matrix secretion on scaffold were observed by scanning electronic microscopy. The tissue-engineered meshes were implanted subcutaneously into the homologous rats while silk fibroin scaffolds served as control. Then the biomechanics, collagen matrix and local tissue response to tissue-engineered meshes in vivo with that to silk fibroin scaffolds were estimated.
  
  
  RESULTS
  ADSC were fibroblast-like and proliferated well in vitro. SEM showed that the scaffold had an interwoven structure with a smooth surface and the silk sponges in scaffold had a uniform and porous structure. ADSC adhered firmly to the scaffold, secreted extracellular matrix and formed cell sheets on scaffold. In vivo studies showed that the tissue matrix of tissue-engineered meshes was better organized than silk fibroin scaffolds. They also had higher failure force (3.081 ± 0.121 vs 2.167 ± 0.148 N) and Young's modulus (3.191 ± 0.146 vs 2.263 ± 0.213 MPa) and more collagen content (18.648 ± 0.867 vs 14.123 ± 0.989 µg/mg) than silk fibroin scaffolds (P < 0.05).
  
  
  CONCLUSIONS
  As a suitable cell type for tissue engineering, ADSC may be successfully isolated and stably proliferated in vitro. The tissue-engineered meshes have an excellent biocompatibility and appropriate properties for pelvic floor reconstruction.
authors.affiliation: Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.forename: Haifeng
authors.initials: H
authors.lastname: Liu
authors.affiliation: Email: wjianliu@netease.com.
authors.forename: Jianliu
authors.initials: J
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Xie
authors.forename: Lihui
authors.initials: L
authors.lastname: Wei
mesh_terms: D000273:Adipose Tissue; D000818:Animals; D049109:Cell Proliferation; D003094:Collagen; D005109:Extracellular Matrix; D005260:Female; D005349:Fibroins; D059630:Mesenchymal Stem Cells; D017773:Pelvic Floor; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures; D047011:Silk; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D047011:Silk; D003094:Collagen; D005349:Fibroins