title: Enhanced production of lovastatin by Omphalotus olearius (DC.) Singer in solid state fermentation.
abstract: BACKGROUND
  Although lovastatin production has been reported for different microorganism species, there is limited information about lovastatin production by basidiomycetes.
  
  
  OBJECTIVE
  The optimization of culture parameters that enhances lovastatin production by Omphalotus olearius OBCC 2002 was investigated, using statistically based experimental designs under solid state fermentation.
  
  
  METHODS
  The Plackett Burman design was used in the first step to test the relative importance of the variables affecting production of lovastatin. Amount and particle size of barley were identified as efficient variables. In the latter step, the interactive effects of selected efficient variables were studied with a full factorial design.
  
  
  RESULTS
  A maximum lovastatin yield of 139.47mg/g substrate was achieved by the fermentation of 5g of barley, 1-2mm particle diam., at 28°C.
  
  
  CONCLUSIONS
  This study showed that O. olearius OBCC 2002 has a high capacity for lovastatin production which could be enhanced by using solid state fermentation with novel and cost-effective substrates, such as barley.
authors.affiliation: Eskişehir Osmangazi University, Graduate School of Natural and Applied Sciences, 26480 Eskişehir, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Atlı
authors.affiliation: Eskişehir Osmangazi University, Faculty of Science and Arts, Department of Biology, 26480 Eskişehir, Turkey. Electronic address: myamac@ogu.edu.tr.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Yamaç
authors.affiliation: Eskişehir Osmangazi University, Faculty of Science and Arts, Department of Statistics, 26480 Eskişehir, Turkey.
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yıldız
authors.affiliation: Mushroom Biology and Fungal Biotechnology Laboratory, North Carolina A&T State University, Greensboro, NC, USA.
authors.forename: Omoanghe S
authors.initials: OS
authors.lastname: Isikhuemnen
mesh_terms: D000363:Agaricales / Q000378:metabolism*; D018554:Avena; D003470:Culture Media; D004043:Dietary Fiber; D005285:Fermentation; D001467:Hordeum; D007218:Industrial Microbiology / Q000379:methods*; D007785:Lactose / Q000494:pharmacology; D008148:Lovastatin / Q000096:biosynthesis*; D008954:Models, Biological; D009172:Mycology / Q000379:methods*; D012275:Oryza; D014131:Trace Elements / Q000494:pharmacology; D003313:Zea mays
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D014131:Trace Elements; D008148:Lovastatin; D007785:Lactose
keywords: Diseño estadístico; Fermentación en estado sólido; Lovastatin; Lovastatina; Omphalotus olearius; Optimización; Optimization; Solid state fermentation; Statistical modeling