title: Sphingobium endophyticus sp. nov., isolated from the root of Hylomecon japonica.
abstract: A yellow-pigmented bacterium, designated strain GZGR-4(T), was isolated from the root of Hylomecon japonica (Thunb.) Prantl et Kündig collected from Taibai Mountain in Shaanxi Province, north-west China. Cells of strain GZGR-4(T) were Gram-negative, rod-shaped, strictly aerobic, non-endospore-forming and non-motile. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GZGR-4(T) is a member of the genus Sphingobium, exhibiting the highest sequence similarity to Sphingobium aromaticiconvertens DSM 12677(T) (97.3 %). 16S rRNA gene sequence similarities between strain GZGR-4(T) and the type strains of other Sphingobium species with validly published names ranged from 93.4-96.5 %. The predominant respiratory quinone of strain GZGR-4(T) was ubiquinone-10 (Q-10) and the major cellular fatty acids were summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C14:0 2-OH. Spermidine was the major polyamine. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified phosphoglycolipid, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The DNA G+C content was 63.6 mol%. DNA-DNA relatedness for strain GZGR-4(T) with respect to its closest phylogenetic relative S. aromaticiconvertens DSM 12677(T) was 22.6 ± 5.3 %. On the basis of the polyphasic taxonomic data presented, strain GZGR-4(T) is considered to represent a novel species of the genus Sphingobium, for which the name Sphingobium endophyticus sp. nov. is proposed. The type strain is GZGR-4(T) (=CCTCC AB 2013305(T) = KCTC 32447(T)).
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Zhu
authors.forename: Kaiyun
authors.initials: K
authors.lastname: Xin
authors.forename: Chaoqiong
authors.initials: C
authors.lastname: Chen
authors.forename: Changfu
authors.initials: C
authors.lastname: Li
authors.forename: Meiru
authors.initials: M
authors.lastname: Si
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Xu
authors.initials: X
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xihui
authors.initials: X
authors.lastname: Shen
mesh_terms: D000332:Aerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D028101:Papaveraceae / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000032:analysis; D018517:Plant Roots / Q000382:microbiology*; D011073:Polyamines / Q000032:analysis; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D042301:Sphingomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011073:Polyamines; D011809:Quinones; D012336:RNA, Ribosomal, 16S