title: Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.
abstract: We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
authors.affiliation: Department of Biomedical Engineering, UNC-Chapel Hill, NC, USA. ssoper@email.unc.edu.
authors.forename: Soumya V
authors.initials: SV
authors.lastname: Nair
authors.forename: Małgorzata A
authors.initials: MA
authors.lastname: Witek
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Jackson
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Lindell
authors.forename: Sally A
authors.initials: SA
authors.lastname: Hunsucker
authors.forename: Travis
authors.initials: T
authors.lastname: Sapp
authors.forename: Caroline E
authors.initials: CE
authors.lastname: Perry
authors.forename: Mateusz L
authors.initials: ML
authors.lastname: Hupert
authors.forename: Victoria
authors.initials: V
authors.lastname: Bae-Jump
authors.forename: Paola A
authors.initials: PA
authors.lastname: Gehrig
authors.forename: Weiya Z
authors.initials: WZ
authors.lastname: Wysham
authors.forename: Paul M
authors.initials: PM
authors.lastname: Armistead
authors.forename: Peter
authors.initials: P
authors.lastname: Voorhees
authors.forename: Steven A
authors.initials: SA
authors.lastname: Soper
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D018952:Antigens, CD34 / Q000276:immunology; D000951:Antigens, Neoplasm / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology; D045744:Cell Line, Tumor; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D010450:Endopeptidases; D000071858:Epithelial Cell Adhesion Molecule; D018093:Gelatinases / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D009360:Neoplastic Cells, Circulating / Q000737:chemistry; D012697:Serine Endopeptidases / Q000276:immunology; D014498:Uracil / Q000737:chemistry*; D051981:Uracil-DNA Glycosidase / Q000737:chemistry*; D000099167:Fibroblast Activation Protein Alpha
chemical_list: D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D004277:DNA, Single-Stranded; D004706:Endodeoxyribonucleases; D010450:Endopeptidases; D000071858:Epithelial Cell Adhesion Molecule; D018093:Gelatinases; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D014498:Uracil; D051981:Uracil-DNA Glycosidase; D000099167:Fibroblast Activation Protein Alpha; C499379:EPCAM protein, human; C035571:endodeoxyribonuclease VII