title: Highly sensitive fluorescence biosensors for sparfloxacin detection at nanogram level based on electron transfer mechanism of cadmium telluride quantum dots.
abstract: A sensitive fluorescence biosensor for determining sparfloxacin (SPF) based on the electron transfer mechanism and the fluorescence quenching effect of SPF to cadmium telluride quantum dots (CdTe QDs) was developed. The mechanism of the interaction between SPF and CdTe QDs was investigated by UV/Vis absorption and fluorescence spectroscopy. The biosensor could be used for the determination of SPF with a high sensitivity. Under optimum conditions, the linear range was from 0.28 to 40 μg SPF ml(-1) with a correlation coefficient of 0.9983, and the detection limit (3δ/k) was 83.7 ng SPF ml(-1). Furthermore, this method has been applied to the determination of SPF in the synthetic environmental water samples and the spiked human serum samples with good results.
authors.affiliation: Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
authors.forename: Wanjun
authors.initials: W
authors.lastname: Liang
authors.forename: Shaopu
authors.initials: S
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Song
authors.forename: Chenxia
authors.initials: C
authors.lastname: Hao
authors.forename: Linlin
authors.initials: L
authors.lastname: Wang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Youqiu
authors.initials: Y
authors.lastname: He
mesh_terms: D000995:Antitubercular Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D005453:Fluorescence; D024841:Fluoroquinolones / Q000032:analysis*; D006801:Humans; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013691:Tellurium / Q000737:chemistry*
chemical_list: D000995:Antitubercular Agents; D019187:Cadmium Compounds; D024841:Fluoroquinolones; D013691:Tellurium; C061363:sparfloxacin; C028337:cadmium telluride