title: Hydrogen sulfide donor protects porcine oocytes against aging and improves the developmental potential of aged porcine oocytes.
abstract: Porcine oocytes that have matured in in vitro conditions undergo the process of aging during prolonged cultivation, which is manifested by spontaneous parthenogenetic activation, lysis or fragmentation of aged oocytes. This study focused on the role of hydrogen sulfide (H2S) in the process of porcine oocyte aging. H2S is a gaseous signaling molecule and is produced endogenously by the enzymes cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MPST). We demonstrated that H2S-producing enzymes are active in porcine oocytes and that a statistically significant decline in endogenous H2S production occurs during the first day of aging. Inhibition of these enzymes accelerates signs of aging in oocytes and significantly increases the ratio of fragmented oocytes. The presence of exogenous H2S from a donor (Na2S.9H2O) significantly suppressed the manifestations of aging, reversed the effects of inhibitors and resulted in the complete suppression of oocyte fragmentation. Cultivation of aging oocytes in the presence of H2S donor positively affected their subsequent embryonic development following parthenogenetic activation. Although no unambiguous effects of exogenous H2S on MPF and MAPK activities were detected and the intracellular mechanism underlying H2S activity remains unclear, our study clearly demonstrates the role of H2S in the regulation of porcine oocyte aging.
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Tereza
authors.initials: T
authors.lastname: Krejcova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Smelcova
authors.affiliation: Institute of Animal Science, Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Petr
authors.affiliation: Université Lille1, Sciences et Technologies, Laboratoire de Régulation des Signaux de Division - EA 4479, Villeneuve d´Ascq, France.
authors.forename: Jean-Francois
authors.initials: JF
authors.lastname: Bodart
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Sedmikova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Nevoral
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Dvorakova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Vyskocilova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Ivona
authors.initials: I
authors.lastname: Weingartova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Kucerova-Chrpova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Chmelikova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Tumova
authors.affiliation: Czech University of Life Sciences in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Prague, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jilek
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D003541:Cystathionine beta-Synthase / Q000378:metabolism*; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006862:Hydrogen Sulfide / Q000378:metabolism / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010312:Parthenogenesis / Q000187:drug effects; D013466:Sulfurtransferases / Q000378:metabolism*; D013552:Swine
chemical_list: D004791:Enzyme Inhibitors; D013466:Sulfurtransferases; C021550:3-mercaptopyruvate sulphurtransferase; D003541:Cystathionine beta-Synthase; D003542:Cystathionine gamma-Lyase; D006862:Hydrogen Sulfide