title: Inhibition effect of graphene oxide on the catalytic activity of acetylcholinesterase enzyme.
abstract: Variations in the enzyme activity of acetylcholinesterase (AChE) in the presence of the nano-material, graphene oxide (GO), were investigated with the use of molecular spectroscopy UV-visible and fluorescence methods. From these studies, important kinetic parameters of the enzyme were extracted; these were the maximum reaction rate, Vm , and the Michaelis constant, Km . A comparison of these parameters indicated that GO inhibited the catalytic activity of the AChE because of the presence of the AChE-GO complex. The formation of this complex was confirmed with the use of fluorescence data, which was resolved with the use of the MCR-ALS chemometrics method. Furthermore, it was found that the resonance light-scattering (RLS) intensity of AChE changed in the presence of GO. On this basis, it was demonstrated that the relationship between AChE and GO was linear and such models were used for quantitative analyses of GO.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China.|Department of Chemistry, Nanchang University, Nanchang, 330031, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang, 330031, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Gu
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China.|Department of Chemistry, Nanchang University, Nanchang, 330031, China.
authors.forename: Yongnian
authors.initials: Y
authors.lastname: Ni
authors.affiliation: School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology, Brisbane, 4001, Australia.
authors.forename: Serge
authors.initials: S
authors.lastname: Kokot
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D055162:Biocatalysis / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006108:Graphite / Q000737:chemistry / Q000494:pharmacology*; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002800:Cholinesterase Inhibitors; D010087:Oxides; D006108:Graphite; D000110:Acetylcholinesterase
keywords: acetylcholinesterase; catalysis; graphene oxide; inhibition; molecular spectroscopy