title: Soluble insulin receptor as a source of insulin resistance and cognitive impairment in HIV-seropositive women.
abstract: Insulin resistance occurs in HIV-infected individuals and is associated with HIV-associated neurocognitive disorders (HAND). However, the mechanisms involved are not well understood. Previously, we showed a correlation between soluble insulin receptor (sIR) and HAND. Here, we investigated if binding of free insulin to sIR and soluble insulin-like growth factor-1 receptor (sIGF1-R) levels are associated with sIR in HAND. Thirty-four (34) HIV-seropositive women stratified by cognitive status and five HIV-seronegative women were evaluated. In a subgroup of 20 HIV positive and 5 donors, binding of plasma insulin to sIR was determined by ELISA assay of residual insulin levels in plasma immuno-depleted with anti-IR-monoclonal antibody-Sepharose beads. sIR and sIGF1-R levels were determined by ELISA. Nonparametric statistics were used. Higher percentages of insulin bound to sIR significantly correlated with sIR levels and were associated with HAND status. Higher levels of plasma sIGF1-R had a positive correlation with sIR levels (p = 0.011) and were associated with HAND (p = 0.006). No correlations were observed with age, viral-immune profile, antiretroviral therapy, or TNF. This study suggests that changes in sIGF1-R levels and insulin binding to sIR may contribute to HAND.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy and NeuroAIDS Program, Specialized Neuroscience Research Program (SNRP), University of Puerto Rico, Medical Sciences Campus, PO Box 365067, San Juan, PR, 00936-5067, USA.
authors.forename: Yamil
authors.initials: Y
authors.lastname: Gerena
authors.forename: Raissa
authors.initials: R
authors.lastname: Menéndez-Delmestre
authors.forename: Richard L
authors.initials: RL
authors.lastname: Skolasky
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Hechavarria
authors.forename: Sebastián
authors.initials: S
authors.lastname: Pérez
authors.forename: Claudia
authors.initials: C
authors.lastname: Hilera
authors.forename: Claribel
authors.initials: C
authors.lastname: González
authors.forename: Avindra
authors.initials: A
authors.lastname: Nath
authors.forename: Valerie
authors.initials: V
authors.lastname: Wojna
mesh_terms: D015526:AIDS Dementia Complex / Q000097:blood / Q000150:complications*; D000328:Adult; D003072:Cognition Disorders / Q000097:blood / Q000209:etiology*; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D008875:Middle Aged; D017526:Receptor, IGF Type 1 / Q000097:blood; D011972:Receptor, Insulin / Q000097:blood*; D012189:Retrospective Studies
chemical_list: D017526:Receptor, IGF Type 1; D011972:Receptor, Insulin