title: Ssn6 has dual roles in Candida albicans filament development through the interaction with Rpd31.
abstract: Ssn6 is a crucial regulator of morphological transition and virulence in the fungal pathogen Candida albicans. Ssn6 has previously been reported to act in complex with the transcriptional repressor Tup1. Here, we report that Ssn6 also interacts with the histone deacetylase Rpd31, independently of Tup1. The ssn6/rpd31 double mutant strain formed elongated filaments, but failed to form filament extension, and this coincided with the down-regulation of the filament extension gene UME6. Occupancy patterns of Ssn6 and Rpd31 differed at the promoters of UME6 and the metabolic gene INO1. These findings indicate that, in C. albicans, Ssn6 has dual roles in filament development, depending on the interaction with Rpd31.
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Jang-Hyun
authors.initials: JH
authors.lastname: Oh
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: MyungHee
authors.initials: M
authors.lastname: Ku
authors.affiliation: Department of Molecular Biosciences, College of Biomedical Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 200-701, Republic of Korea.
authors.forename: Jueun
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Molecular Biosciences, College of Biomedical Sciences, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon-si, Gangwon-do 200-701, Republic of Korea. Electronic address: jungshinlee@kangwon.ac.kr.
authors.forename: Jung-Shin
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, Republic of Korea. Electronic address: kangsaou@snu.ac.kr.
authors.forename: Sa-Ouk
authors.initials: SO
authors.lastname: Kang
mesh_terms: D002176:Candida albicans / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005656:Fungal Proteins / Q000502:physiology*; D015966:Gene Expression Regulation, Fungal; D006655:Histone Deacetylases / Q000378:metabolism*; D025301:Hyphae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012097:Repressor Proteins / Q000502:physiology*; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D012097:Repressor Proteins; D006655:Histone Deacetylases
keywords: Candida albicans; Filamentous growth; Rpd31; Ssn6; Transcriptional repressor