title: Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex.
abstract: A dinuclear ruthenium(II) complex [(bpy)2Ru(L(1))Ru(bpy)2]Cl4 {bpy=2,2'-bipyridine, L(1)=1,6-bis(3-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)-9H-carbazol-9-yl)hexane} was synthesised and characterized. The calf thymus DNA (ct-DNA) binding properties of the complex were investigated by means of UV-Visible absorption and emission spectrophotometric titrations, ethidium bromide competitive binding, steady-state emission quenching with ferrocyanide, DNA viscosity measurements, and DNA thermal denaturation. The results indicated that the complex avidly binds to ct-DNA most probably through a threading bis-intercalative mode. The complex was also evidenced to act as an efficient DNA photocleaver, and an effective luminescent stain for cytoplasmic of HeLa cells with low cytotoxicity.
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials and College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Beijing Normal University, Beijing 100875, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials and College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials and College of Chemistry, Beijing Normal University, Beijing 100875, China.
authors.forename: Bao-Yan
authors.initials: BY
authors.lastname: Wu
authors.affiliation: Beijing Key Laboratory of Energy Conversion and Storage Materials and College of Chemistry, Beijing Normal University, Beijing 100875, China. Electronic address: kzwang@bnu.edu.cn.
authors.forename: Ke-Zhi
authors.initials: KZ
authors.lastname: Wang
mesh_terms: D015082:2,2'-Dipyridyl / Q000737:chemistry; D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D053837:DNA Cleavage / Q000187:drug effects* / Q000528:radiation effects; D004996:Ethidium / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D012428:Ruthenium / Q000737:chemistry*; D014783:Viscosity
chemical_list: D056831:Coordination Complexes; D015082:2,2'-Dipyridyl; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium