title: Characterization of co-purified acellular pertussis vaccines.
abstract: Whole-cell pertussis vaccines (WPVs) have been completely replaced by the co-purified acellular vaccines (APVs) in China. To date few laboratory studies were reported for co-purified APVs in terms of their antigenic composition and protective immune responses. To further understand the antigenic composition in co-purified APVs, in the present study 2-dimensional gel electrophoresis-based proteomic technology was used to analyze the composition of co-purified APVs. The results showed that besides the main antigens pertussis toxin (PT) and filamentous hemagglutinin (FHA), co-purified APVs also contained pertactin (PRN), fimbriae (FIM) 2and3 and other minor protein antigens. Of the 9 proteins identified, 3 were differentially presented in products from manufacturer 1 and manufacturer 2. Compared with WPVs and purified APVs, co-purified APVs induced a mixed Th1/Th2 immune response with more toward to a Th1 response than the purified APVs in this study. These results hint that different immune mechanisms might be involved in protection induced by co-purified and purified APVs.
authors.affiliation: a Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products ; National Institutes for Food and Drug Control ; Beijing , PR China ;
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Xu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Tan
authors.forename: Catpagavalli
authors.initials: C
authors.lastname: Asokanathan
authors.forename: Shumin
authors.initials: S
authors.lastname: Zhang
authors.forename: Dorothy
authors.initials: D
authors.lastname: Xing
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis*; D002681:China; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D051379:Mice; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D020543:Proteome / Q000032:analysis; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D014917:Whooping Cough / Q000517:prevention & control*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D010567:Pertussis Vaccine; D020543:Proteome; D022282:Vaccines, Acellular
keywords: 2-DE, 2-dimensional gel electrophoresis; APVs, acellular pertussis vaccines; BipA, putative outer membrane ligand binding protein; Co-purified acellular pertussis vaccines; FHA, filamentous hemagglutinin; FIM, fimbriae; PRN, pertactin; PT, pertussis toxin; SHD, single human dose; Sbp, sulfate-binding protein precursor; WPVs, whole-cell pertussis vaccines; bvg, Bordetella virulence regulon; immune responses; protection; proteomic analysis; sphB1, autotransporter subtilisin-like protease