title: Surface enhanced vibrational spectroscopic evidence for an alternative DNA-independent redox activation of endonuclease III.
abstract: Surface enhanced vibrational spectro-electrochemistry of endonuclease III provides direct evidence that the [4Fe-4S] cluster is responsible for the enzyme redox activity, and that this process is not exclusively DNA-mediated, as currently proposed. We report the first surface enhanced resonance Raman spectrum of a [4Fe-4S](2+) cluster containing enzyme.
authors.affiliation: Instituto de Tecnologia Quimica e Biologica (ITQB), Universidade Nova de Lisboa, Av. da Republica (EAN), 2780-157 Oeiras, Portugal. smilja@itqb.unl.pt.
authors.forename: Elin
authors.initials: E
authors.lastname: Moe
authors.forename: Murat
authors.initials: M
authors.lastname: Sezer
authors.forename: Peter
authors.initials: P
authors.lastname: Hildebrandt
authors.forename: Smilja
authors.initials: S
authors.lastname: Todorovic
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D034301:Deinococcus / Q000201:enzymology; D004563:Electrochemistry; D004720:Endonucleases / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman; D013455:Sulfur / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D013455:Sulfur; D007501:Iron; D004720:Endonucleases