title: Structural basis of a temporin 1b analogue antimicrobial activity against Gram negative bacteria determined by CD and NMR techniques in cellular environment.
abstract: We here report an original approach to elucidate mechanisms of action of antimicrobial peptides and derive crucial structural requirements for the design of novel therapeutic agents. The high resolution structure of TB_KKG6A, an antimicrobial peptide designed to amplify the spectrum of action of Temporin B, bound to E. coli is here determined by means of CD and NMR methodologies. We have also defined, through STD analysis, the residues in closer proximity to the bacterial membrane.
authors.affiliation: †Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Malgieri
authors.affiliation: ‡Diagnostica e Farmaceutiche Molecolari Scarl, Via Mezzocannone 16,80134 Napoli, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Avitabile
authors.affiliation: †Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Maddalena
authors.initials: M
authors.lastname: Palmieri
authors.affiliation: §Istituto di Biostrutture e Bioimmagini, CNR, Via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Luca Domenico
authors.initials: LD
authors.lastname: D'Andrea
authors.affiliation: †Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Isernia
authors.affiliation: ∥Dipartimento di Farmacia, Università di Napoli Federico II, Via Mezzocannone 16, 80134 Napoli, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
authors.affiliation: †Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università degli studi di Napoli, Via Vivaldi 43, 81100 Caserta, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Fattorusso
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002942:Circular Dichroism; D015195:Drug Design; D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D023181:Antimicrobial Cationic Peptides; D011506:Proteins; C104501:temporin