title: Ozonation of sunflower oils: impact of experimental conditions on the composition and the antibacterial activity of ozonized oils.
abstract: Ozone can react with vegetable oils to produce ozonized oils which have antimicrobial properties and can be used in dermatology. The aim of this study was to evaluate the influence of ozonation conditions and of the initial fatty acid composition on iodine index (II), peroxide index (IP), acidity value (AV) of ozonized sunflower oils. The antibacterial activity of these products against the three bacterial strains that are more often involved in mastitis (Staphylococcus aureus, Escherichia coli and Streptococcus uberis) was also evaluated. In that purpose, two different sunflower oils have been studied: a "classical" oil (55% linoleic acid, 35% oleic acid) and a "high oleic" oil (90% oleic acid). Both were ozonized with or without water during different times (from 1 to 7 h). Results show that the addition of water has a direct impact on the increase in IP (up to 2600 meq of active oxygen/kg of oil with water and 430 without) and AV but does not influence the kinetic of the decrease in II. Minimal inhibitory concentrations were ranging from 1.25 to 40 mg/mL and the antibacterial activity of oils ozonized with water was better than the one of oils ozonized alone. These results are an open door to new applications of ozonized oils.
authors.affiliation: Université de Toulouse, INP-Ecole d'Ingénieurs de Purpan, Laboratoire de Chimie Agro-Industrielle, 75, voie du TOEC, BP 57611, 31076 Toulouse Cedex 03, France; INRA, UMR 1010 CAI, 31030 Toulouse, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Moureu
authors.affiliation: Université de Toulouse, INP-Ecole d'Ingénieurs de Purpan, Laboratoire de Chimie Agro-Industrielle, 75, voie du TOEC, BP 57611, 31076 Toulouse Cedex 03, France; INRA, UMR 1010 CAI, 31030 Toulouse, France. Electronic address: frederic.violleau@purpan.fr.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Violleau
authors.affiliation: Université de Toulouse, INP-Ecole d'Ingénieurs de Purpan, Equipe Systèmes de Productions Agricoles, 75, voie du TOEC, BP 57611, 31076 Toulouse Cedex 03, France.
authors.forename: Djamila
authors.initials: D
authors.lastname: Ali Haimoud-Lekhal
authors.affiliation: Université de Toulouse, INP-Ecole d'Ingénieurs de Purpan, Laboratoire de Chimie Agro-Industrielle, 75, voie du TOEC, BP 57611, 31076 Toulouse Cedex 03, France; INRA, UMR 1010 CAI, 31030 Toulouse, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Calmon
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008413:Mastitis / Q000188:drug therapy / Q000382:microbiology*; D010126:Ozone / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects; D000074242:Sunflower Oil
chemical_list: D000900:Anti-Bacterial Agents; D010938:Plant Oils; D000074242:Sunflower Oil; D010126:Ozone
keywords: Antibacterial activity; Fatty acid composition; Mastitis; Ozonation conditions; Ozonized sunflower oil