title: Chip-based free-flow electrophoresis with integrated nanospray mass-spectrometry.
abstract: Free-flow electrophoresis is an ideal tool for preparative separations in continuous microflow. With the approach presented herein for coupling free-flow electrophoresis and mass spectrometry it is now also possible to trace non-fluorescent compounds and identify them by means of mass spectrometry. The functionality of the method and its potential as an integrated separation unit for microflow synthesis is demonstrated by application to a multicomponent [3+2]-cycloannulation.
authors.affiliation: Institut für Analytische Chemie, Universität Leipzig, Linnéstrasse 3, 04103 Leipzig (Germany); LIFE-Leipzig Research Center for Civilization Diseases, Universität Leipzig (Germany).
authors.forename: Christian
authors.initials: C
authors.lastname: Benz
authors.forename: Michael
authors.initials: M
authors.lastname: Boomhoff
authors.forename: Johannes
authors.initials: J
authors.lastname: Appun
authors.forename: Christoph
authors.initials: C
authors.lastname: Schneider
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D001547:Benzaldehydes / Q000138:chemical synthesis / Q000737:chemistry; D003500:Cyclization; D004586:Electrophoresis / Q000379:methods*; D013058:Mass Spectrometry; D015394:Molecular Structure; D036103:Nanotechnology; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry; D062367:ortho-Aminobenzoates / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D001547:Benzaldehydes; D017646:Organosilicon Compounds; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; C032175:benzaldehyde
keywords: continuous micro flow-through; cycloaddition; electrophoresis; mass spectrometry; microfluidics