title: High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues.
abstract: An imaging platform based on broadband coherent anti-Stokes Raman scattering (BCARS) has been developed which provides an advantageous combination of speed, sensitivity and spectral breadth. The system utilizes a configuration of laser sources that probes the entire biologically-relevant Raman window (500 cm-1to 3500 cm-1) with high resolution (< 10 cm-1). It strongly and efficiently stimulates Raman transitions within the typically weak "fingerprint" region using intrapulse 3-colour excitation, and utilizes the nonresonant background (NRB) to heterodyne amplify weak Raman signals. We demonstrate high-speed chemical imaging in two- and three-dimensional views of healthy murine liver and pancreas tissues and interfaces between xenograft brain tumours and the surrounding healthy brain matter.
authors.affiliation: Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: Charles H
authors.initials: CH
authors.lastname: Camp
authors.affiliation: Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: Young Jong
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Semiconductor and Dimensional Metrology Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: John M
authors.initials: JM
authors.lastname: Heddleston
authors.affiliation: Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hartshorn
authors.affiliation: Semiconductor and Dimensional Metrology Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: Angela R
authors.initials: AR
authors.lastname: Hight Walker
authors.affiliation: Department of Stem Cell Biology and Regenerative Medicine, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
authors.forename: Jeremy N
authors.initials: JN
authors.lastname: Rich
authors.affiliation: Department of Cellular and Molecular Medicine, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA.
authors.forename: Justin D
authors.initials: JD
authors.lastname: Lathia
authors.affiliation: Biosystems and Biomaterials Division, National Institute of Standards and Technology, 100 Bureau Dr, Gaithersburg, MD 20899, USA.
authors.forename: Marcus T
authors.initials: MT
authors.lastname: Cicerone