title: Interplay of H2A deubiquitinase 2A-DUB/Mysm1 and the p19(ARF)/p53 axis in hematopoiesis, early T-cell development and tissue differentiation.
abstract: Monoubiquitination of core histone 2A (H2A-K119u) has a critical role in gene regulation in hematopoietic differentiation and other developmental processes. To explore the interplay of histone H2A deubiquitinase Myb-like SWIRM and MPN domain containing1 (2A-DUB/Mysm1) with the p53 axis in the sequential differentiation of mature lymphocytes from progenitors, we systematically analyzed hematopoiesis and early T-cell development using Mysm1(-/-) and p53(-/-)Mysm1(-/-) mice. Mysm1(-/-) thymi were severely hypoplastic with <10% of wild-type cell numbers as a result of a reduction of early thymocyte progenitors in context with defective hematopoietic stem cells, a partial block at the double-negative (DN)1-DN2 transition and increased apoptosis of double-positive thymocytes. Increased rates of apoptosis were also detected in other tissues affected by Mysm1 deficiency, including the developing brain and the skin. By quantitative PCR and chromatin immunoprecipitation analyses, we identified p19(ARF), an important regulator of p53 tumor suppressor protein levels, as a potential Mysm1 target gene. In newly generated p53(-/-)Mysm1(-/-) double-deficient mice, anomalies of Mysm1(-/-) mice including reduction of lymphoid-primed multipotent progenitors, reduced thymocyte numbers and viability, and interestingly defective B-cell development, growth retardation, neurological defects, skin atrophy, and tail malformation were almost completely restored as well, substantiating the involvement of the p53 pathway in the alterations caused by Mysm1 deficiency. In conclusion, this investigation uncovers a novel link between H2A deubiquitinase 2A-DUB/Mysm1 and suppression of p53-mediated apoptotic programs during early lymphoid development and other developmental processes.
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Gatzka
authors.affiliation: Institute of Immunology, University of Ulm, Ulm 89091, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Tasdogan
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hainzl
authors.affiliation: Institute of Immunology, University of Ulm, Ulm 89091, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Allies
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wilms
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wlaschek
authors.affiliation: Department of Dermatology and Allergic Diseases, University of Ulm, Ulm 89091, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Scharffetter-Kochanek
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D050764:Cyclin-Dependent Kinase Inhibitor p19 / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D020869:Gene Expression Profiling; D006410:Hematopoiesis / Q000502:physiology*; D006657:Histones / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013601:T-Lymphocytes / Q000166:cytology* / Q000378:metabolism; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D064570:Ubiquitin-Specific Proteases
chemical_list: D050764:Cyclin-Dependent Kinase Inhibitor p19; D006657:Histones; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53; C475961:gamma-H2AX protein, mouse; D010450:Endopeptidases; C570173:MYSM1 protein, mouse; D064570:Ubiquitin-Specific Proteases