title: A dansyl-rhodamine ratiometric fluorescent probe for Hg2+ based on FRET mechanism.
abstract: Based on resonance energy transfer (FRET) from dansyl to rhodamine 101, a new fluorescent probe (compound 1) containing rhodamine 101 and a dansyl unit was synthesized for detecting Hg(2+) through ratiometric sensing in DMSO aqueous solutions. This probe shows a fast, reversible and selective response toward Hg(2+) in a wide pH range. Hg(2+) induced ring-opening reactions of the spirolactam rhodamine moiety of 1, leading to the formation of fluorescent derivatives that can serve as the FRET acceptors. Very large stokes shift (220 nm) was observed in this case. About 97-fold increase in fluorescence intensity ratio was observed upon its binding with Hg(2+).
authors.affiliation: College of Sciences, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China, phxie2013@163.com.
authors.forename: Puhui
authors.initials: P
authors.lastname: Xie
authors.forename: Fengqi
authors.initials: F
authors.lastname: Guo
authors.forename: Lingyu
authors.initials: L
authors.lastname: Wang
authors.forename: Sen
authors.initials: S
authors.lastname: Yang
authors.forename: Denghui
authors.initials: D
authors.lastname: Yao
authors.forename: Guoyu
authors.initials: G
authors.lastname: Yang
mesh_terms: D003619:Dansyl Compounds / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D013997:Time Factors
chemical_list: D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; D008628:Mercury