title: [Establishment of a NOD/SCID mouse model with human immune reconstitution bearing human triple-negative breast cancer].
abstract: OBJECTIVE
  To establish a NOD/SCID mouse model with human immune reconstitution and observe its immune response to human triple-negative breast cancer xenograft.
  
  
  METHODS
  Twenty-four NOD/SCID mice without immune leakage were subjected to cyclophosphamide (CTX) treatment 3 days prior to immune reconstitution with human peripheral blood mononuclear cell (PBMC) injection and subcutaneous transplantation of human triple-negative breast cancer MDA-MB-231 cells, CTX treatment and PBMC injection without tumor cell transplantation, MDA-MB-231 cell transplantation only, or no treatments. The tumor growth and immune responses of the mice were observed at regular intervals.
  
  
  RESULTS
  Compared with the tumor-bearing mice, the tumor-bearing mice with immune reconstitution showed prolonged incubation period of tumor formation, slower tumor growth rate and increased survival rate. Human IgG and CD3(+) T cells were detected in the peripheral blood of the mice 1 week after human PBMC injection. The percentage of CD3(+) T cells in the spleen cells was 55.3% at 9 weeks in tumor-bearing mice with immune reconstitution and 52.7% in tumor-bearing mice without immune reconstitution. The spleen index of the tumor-bearing mice with immune reconstitution was much higher than that in mice with only immune reconstitution and the control mice (9.64 vs 3.82∓0.31 and 1.51∓0.14 mg/g).
  
  
  CONCLUSIONS
  A stable NOD/SCID mouse model with immune reconstitution has been established successfully, which shows immune responses to triple-negative breast cancer xenografts and allows studies of immunological therapy study of triple-negative breast cancer.
authors.affiliation: Department of Thyroid and Breast Surgery, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China.E-mail: zhangqiaochuaz@163.com.
authors.forename: Qiao-Chu
authors.initials: QC
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.forename: Rui-Lei
authors.initials: RL
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Ze-Nan
authors.initials: ZN
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Mi
authors.initials: M
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007963:Leukocytes, Mononuclear; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D013154:Spleen / Q000276:immunology; D064726:Triple Negative Breast Neoplasms / Q000276:immunology*