title: Specific aptamer-conjugated mesoporous silica-carbon nanoparticles for HER2-targeted chemo-photothermal combined therapy.
abstract: Tumor-specific therapeutic platforms designed for combined tumor therapy has recently received wide attention. In this work, a new HB5 aptamer-functionalized mesoporous silica-carbon based doxorubicin (DOX)-loaded system (MSCN-PEG-HB5/DOX) was successfully constructed and characterized for chemo-photothermal combined therapy of human epithelial growth factor receptor 2 (HER2)-positive breast cancer cells. The in vitro release result showed that MSCN-PEG-HB5/DOX exhibited pH-sensitive and NIR-triggered release manner. HB5-modified nanoparticles showed significant higher cellular uptake in HER2-positive breast cancer cells (SK-BR-3) but not in normal breast epithelial cells (MCF-10A), compared to unmodified counterparts. The intracellular uptake of functional nanoparticles was mainly based on the receptor-mediated mechanism which was energy-dependent. Cytotoxicity experiments demonstrated that combined therapy induced highest cell killing effect compared to chemotherapy and photothermal therapy alone. The combination index (CI) was 0.253 indicating the synergistic effect of chemotherapy and photothermal therapy. These findings suggested that MSCN-PEG-HB5/DOX was a potential chemo-photothermal therapeutic platform targeting to HER2-positive breast cancers.
authors.affiliation: School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Kaiyuan
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.affiliation: School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Meng
authors.affiliation: Center of Analysis and Measurement, Fudan University, Shanghai 200433, China. Electronic address: wangyifd@fudan.edu.cn.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China. Electronic address: xyyanucm@163.com.
authors.forename: Xueying
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China. Electronic address: rqhuang@fudan.edu.cn.
authors.forename: Rongqin
authors.initials: R
authors.lastname: Huang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003131:Combined Modality Therapy; D004317:Doxorubicin / Q000494:pharmacology; D005260:Female; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006801:Humans; D006979:Hyperthermia, Induced; D020128:Inhibitory Concentration 50; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010778:Photochemotherapy; D016062:Porosity; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D012822:Silicon Dioxide / Q000737:chemistry*; D055672:Static Electricity; D013696:Temperature
chemical_list: D052157:Aptamers, Nucleotide; D002244:Carbon; D004317:Doxorubicin; D016650:Fluorescein-5-isothiocyanate; D018719:Erb-b2 Receptor Tyrosine Kinases; D012822:Silicon Dioxide
keywords: Breast cancer; Chemo-photothermal therapy; HB5 aptamer; HER2-positive; Mesoporous silica–carbon nanoparticles