title: Uroplakin II (UPII), GATA3, and p40 are Highly Sensitive Markers for the Differential Diagnosis of Invasive Urothelial Carcinoma.
abstract: Distinguishing between invasive urothelial carcinoma from other genitourinary lesions such as prostatic and renal carcinomas can be difficult, and may require highly sensitive immunohistochemical markers. GATA-binding protein 3 (GATA3) has been reported in a high percentage of urothelial and breast carcinomas. Mouse monoclonal uroplakin II (UPII) and p40 antibodies have recently been developed and demonstrated high specificity in urothelial carcinoma. This study evaluated the immunohistochemical staining sensitivities of UPII, GATA3, p40, and p63 in the detection of invasive urothelial carcinoma. UPII, GATA3, and p40 were further tested for specificity in lung, breast, colon, kidney, and prostate cancers. In all invasive urothelial carcinoma cases, UPII, GATA3, p40, and p63 exhibited sensitivities of 77.7%, 83.5%, 85.4%, and 80.6%, respectively. The combination of UPII, GATA3, and p40 antibodies stained 94.2% (97/103) of all invasive urothelial carcinoma cases, including 92.2% (71/77) of grade 2-3 urothelial carcinomas. In addition, GATA3 and UPII showed negative staining in lung squamous cell carcinomas and p40 showed negative staining in breast infiltrating ductal carcinomas. The combination of UPII, GATA3, and p40 showed negative staining in lung adenocarcinoma, colon adenocarcinoma, and renal carcinomas. In conclusion, UPII, GATA3, and p40, when used in combination, are highly sensitive in the differential diagnosis of invasive urothelial carcinoma.
authors.affiliation: *Department of Research and Development, Biocare Medical LLC, Concord, CA †Mercy Hospital & Trauma Center, Mercy Health System, Janesville, WI ‡Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Hoang
authors.forename: David
authors.initials: D
authors.lastname: Tacha
authors.forename: Ryan E
authors.initials: RE
authors.lastname: Bremer
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Haas
authors.forename: Liang
authors.initials: L
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D014408:Biomarkers, Tumor / Q000276:immunology*; D003937:Diagnosis, Differential; D005260:Female; D050990:GATA3 Transcription Factor / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D014157:Transcription Factors / Q000276:immunology*; D025521:Tumor Suppressor Proteins / Q000276:immunology*; D014571:Urologic Neoplasms / Q000276:immunology / Q000473:pathology; D060170:Uroplakin II / Q000276:immunology*; D019459:Urothelium / Q000276:immunology / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D050990:GATA3 Transcription Factor; C494749:GATA3 protein, human; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C554658:UPK2 protein, human; D060170:Uroplakin II