title: Prevalence of Acinetobacter baumannii harboring ISAba1/bla OXA-23-like family in a burn center.
abstract: BACKGROUND
  Refractory carbapenem resistant Acinetobacter baumannii (CRAB) isolates increase mortality and morbidity rates among patients with underlying disorders, especially in patients with burns. The aim of the current study was to understand the resistant determinants of CRAB isolates and their clonal relatedness collected from referral Burn center.
  
  
  METHODS
  CRAB isolates were initially characterized and then antimicrobial susceptibility testing was assessed by E-test. Resistance determinants were investigated by PCR. Repetitive extragenic palindromic elements-PCR (REP-PCR) was used for clonality relatedness among isolates.
  
  
  RESULTS
  Thirty CRAB isolates were collected during the study. Colistin was the most effective antibiotic, but, all of the isolates were resistant to carbapenems. intI1 was detected in two isolates and MBLs and gene cassettes were not detected. ISAba1, blaOXA-51, blaOXA-23 and ISAba1/blaOXA23-like were detected in all, while blaOXA-24-like were present in 73% and blaOXA-58&OXA-143 were not present in isolates. REP-PCR demonstrated three clusters, with the dominant B cluster, which contained 16 subgroups.
  
  
  CONCLUSIONS
  CRAB harboring ISAba1/blaOXA-23-like family is widely disseminated in the studied Burn ward setting and the emergence of infection control measures should be regarded to limit refractory CRABs.
authors.affiliation: Student Research Committee, Department of Microbiology & Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Microbiology and Virology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran; Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Himen
authors.initials: H
authors.lastname: Salimizand
authors.affiliation: Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Noori
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: meshkatz@mums.ac.ir.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Meshkat
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Kiarash
authors.initials: K
authors.lastname: Ghazvini
authors.affiliation: Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Saeid Jamehdar
authors.initials: SJ
authors.lastname: Amel
mesh_terms: D000151:Acinetobacter Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D002053:Burn Units; D002056:Burns / Q000150:complications / Q000382:microbiology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016056:Immunodominant Epitopes; D007223:Infant; D020071:Interspersed Repetitive Sequences; D055029:Inverted Repeat Sequences; D007492:Iran / Q000453:epidemiology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D055815:Young Adult; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D016056:Immunodominant Epitopes; C466679:beta-lactamase OXA-23; D001618:beta-Lactamases; C410345:beta-lactamase OXA-24; C577373:beta-lactamase OXA-51, Acinetobacter baumannii
keywords: Acinetobacter baumannii; Burn; ISAba1/blaOXA23-like; REP-PCR