title: Proteomics. Tissue-based map of the human proteome.
abstract: Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden. Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2970 Hørsholm, Denmark. mathias.uhlen@scilifelab.se.
authors.forename: Mathias
authors.initials: M
authors.lastname: Uhlén
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Linn
authors.initials: L
authors.lastname: Fagerberg
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden. Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Björn M
authors.initials: BM
authors.lastname: Hallström
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lindskog
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Per
authors.initials: P
authors.lastname: Oksvold
authors.affiliation: Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Adil
authors.initials: A
authors.lastname: Mardinoglu
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Åsa
authors.initials: Å
authors.lastname: Sivertsson
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Caroline
authors.initials: C
authors.lastname: Kampf
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden. Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Evelina
authors.initials: E
authors.lastname: Sjöstedt
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Anna
authors.initials: A
authors.lastname: Asplund
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: IngMarie
authors.initials: I
authors.lastname: Olsson
authors.affiliation: Leibniz Research Centre for Working Environment and Human Factors (IfADo) at Dortmund TU, D-44139 Dortmund, Germany.
authors.forename: Karolina
authors.initials: K
authors.lastname: Edlund
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Emma
authors.initials: E
authors.lastname: Lundberg
authors.affiliation: Lab Surgpath, Mumbai, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Navani
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Cristina Al-Khalili
authors.initials: CA
authors.lastname: Szigyarto
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Jacob
authors.initials: J
authors.lastname: Odeberg
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Dijana
authors.initials: D
authors.lastname: Djureinovic
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Jenny Ottosson
authors.initials: JO
authors.lastname: Takanen
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Sophia
authors.initials: S
authors.lastname: Hober
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Tove
authors.initials: T
authors.lastname: Alm
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Per-Henrik
authors.initials: PH
authors.lastname: Edqvist
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Holger
authors.initials: H
authors.lastname: Berling
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Hanna
authors.initials: H
authors.lastname: Tegel
authors.affiliation: Science for Life Laboratory, Department of Neuroscience, Karolinska Institute, SE-171 77 Stockholm, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Mulder
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Rockberg
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Nilsson
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Jochen M
authors.initials: JM
authors.lastname: Schwenk
authors.affiliation: Department of Proteomics, KTH-Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
authors.forename: Marica
authors.initials: M
authors.lastname: Hamsten
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Kalle
authors.initials: K
authors.lastname: von Feilitzen
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Mattias
authors.initials: M
authors.lastname: Forsberg
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Lukas
authors.initials: L
authors.lastname: Persson
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Fredric
authors.initials: F
authors.lastname: Johansson
authors.affiliation: Science for Life Laboratory, KTH-Royal Institute of Technology, SE-171 21 Stockholm, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Zwahlen
authors.affiliation: Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: von Heijne
authors.affiliation: Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2970 Hørsholm, Denmark. Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden.
authors.forename: Jens
authors.initials: J
authors.lastname: Nielsen
authors.affiliation: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-751 85 Uppsala, Sweden·
authors.forename: Fredrik
authors.initials: F
authors.lastname: Pontén
mesh_terms: D017398:Alternative Splicing; D002460:Cell Line; D030562:Databases, Protein; D005260:Female; D005796:Genes; D005815:Genetic Code; D006801:Humans; D020407:Internet; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics / Q000378:metabolism; D040081:Protein Array Analysis; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D020033:Protein Isoforms; D020543:Proteome