title: Whole-organism screening for modulators of fasting metabolism using transgenic zebrafish.
abstract: Organismal energy homeostasis is maintained by complex interorgan communications making the discovery of novel drugs against metabolic diseases challenging using traditional high-throughput approaches in vitro. Here, we describe a method that rapidly identifies small molecules with an impact on organismal energy balance in vivo. Specifically, we developed a whole-organism screen for modulators of fasting metabolism using transgenic bioluminescence-reporter zebrafish for the gluconeogenic gene phosphoenolpyruvate-carboxykinase 1 (pck1).
authors.affiliation: Nestlé Institute of Health Sciences S.A., EPFL Innovation Park, Bâtiments (Buildings) G & H, 1015, Lausanne, Switzerland, philipp.gut@ucsf.edu.
authors.forename: Philipp
authors.initials: P
authors.lastname: Gut
authors.forename: Didier Y R
authors.initials: DY
authors.lastname: Stainier
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004734:Energy Metabolism / Q000187:drug effects*; D005215:Fasting / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D054852:Small Molecule Libraries; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism*
chemical_list: D054852:Small Molecule Libraries