title: Ultrastructural features of human adipose-derived multipotent mesenchymal stromal cells.
abstract: Multipotent mesenchymal stromal cells (MMSCs) are plastic-adherent cells with a well-established phenotype. Equine, but not human, adipose MMSCs have been characterized ultrastructurally. The purpose of our study was to evaluate ultrastructurally the adipose-derived human MMSCs. Cell cultures were prepared from human lipoaspirate. The flow cytometry evaluation of surface markers of cultured cells confirmed the expected profile of MMSCs, that were positive for CD73, CD90 and CD105, and negative for CD34 and CD45. We examined these human adipose-derived MMSCs in transmission electron microscopy (TEM) by Epon en-face embedding the fixed MMSCs. The main ultrastructural features of MMSCs were the extremely rich content of endosomal/vesicular elements, long mitochondria, dilated RER (rough endoplasmic reticulum) cisternae, and abundant intermediate filaments and microtubules. We found two types of MMSCS prolongations: (a) thick processes, with opposite, vesicular and filaments-rich, sides and (b) slender processes (pseudopodes and filopodes), with occasional proximal dilated segments housing mitochondria, vesicles and secretory granules. These TEM features of MMSCs characterized an in vitro cell population and could use to distinguish between different cell types in culture.
authors.affiliation: Discipline of Anatomy, Faculty of Dental Medicine, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania; anatomon@gmail.com.
authors.forename: Claudiu Marius
authors.initials: CM
authors.lastname: Manea
authors.forename: Mugurel Constantin
authors.initials: MC
authors.lastname: Rusu
authors.forename: Daniel
authors.initials: D
authors.lastname: Constantin
authors.forename: Valentina Mariana
authors.initials: VM
authors.lastname: Mănoiu
authors.forename: Lucia
authors.initials: L
authors.lastname: Moldovan
authors.forename: Adelina Maria
authors.initials: AM
authors.lastname: Jianu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007091:Image Processing, Computer-Assisted; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000648:ultrastructure*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000648:ultrastructure*