title: Toll-like receptor 9 gene polymorphism in chronic and aggressive periodontitis patients.
abstract: OBJECTIVE
  Periodontitis is a multifactorial disease, with microbial dental plaque as the primary etiological factor. However, the manifestation and progression of periodontitis is influenced by a wide variety of other determinants and factors such as social and behavioral factors, systemic factors, microbial composition of dental plaque, genetic, and many other emerging risk factors. The aim of this study was to analyze genetic polymorphisms in the toll-like receptor 9 (TLR9) gene at - 1237C/T and its association with chronic and generalized aggressive periodontitis (GAgP) in an Indian population.
  
  
  METHODS
  This study was carried out on 90 subjects, which included 30 GAgP and 30 chronic periodontitis patients and 30 healthy controls. Within the limitations of our study, only 30 subjects were included in each group due to the low prevalence of GAgP patients. Blood samples were drawn from the subjects and analyzed for TLR9 genetic polymorphism at - 1237C/T by using polymerase chain reaction-restriction fragment length polymorphism method.
  
  
  RESULTS
  No significant difference was found in genotype and allele frequency of TLR9 genetic polymorphism (- 1237C/T) in generalized aggressive and chronic periodontitis patients and healthy controls.
  
  
  CONCLUSIONS
  Toll-like receptor 9 genetic polymorphism at - 1237C/T may not be associated with GAgP and chronic periodontitis patients in Indian population.
authors.affiliation: Department of Periodontics, Alfarabi College of Dentistry, Riyadh, Saudi Arabia.
authors.forename: Nipun
authors.initials: N
authors.lastname: Ashok
authors.affiliation: Department of Oral and Maxillofacial Sciences, P.M.N.M Dental College and Hospital, Bagalkot, India.
authors.forename: Shivaraj
authors.initials: S
authors.lastname: Warad
authors.affiliation: Department of Oral and Maxillofacial Sciences, P.M.N.M Dental College and Hospital, Bagalkot, India.
authors.forename: Nagaraj Balasaheb
authors.initials: NB
authors.lastname: Kalburgi
authors.affiliation: Department of Oral and Maxillofacial Sciences, Rajarajeshwari Dental College and Hospital, Bengaluru, Karnataka, India.
authors.forename: Shivaprasad
authors.initials: S
authors.lastname: Bilichodmath
authors.affiliation: Department of Endodontics, Amritha Institute of Dental Science, Cochin, Kerala, India.
authors.forename: Prabath Singh Valiyaparambil
authors.initials: PS
authors.lastname: Prabhakaran
authors.affiliation: Department of Oral and Maxillofacial Sciences, Alfarabi College of Dentistry, Riyadh, Saudi Arabia.
authors.forename: Bassel
authors.initials: B
authors.lastname: Tarakji
keywords: Aggressive periodontitis; chronic periodontitis; genetic polymorphism; periodontal risk factor; polymerase chain reaction; toll-like receptor 9