title: Membrane curvature enables N-Ras lipid anchor sorting to liquid-ordered membrane phases.
abstract: Trafficking and sorting of membrane-anchored Ras GTPases are regulated by partitioning between distinct membrane domains. Here, in vitro experiments and microscopic molecular theory reveal membrane curvature as a new modulator of N-Ras lipid anchor and palmitoyl chain partitioning. Membrane curvature was essential for enrichment in raft-like liquid-ordered phases; enrichment was driven by relief of lateral pressure upon anchor insertion and most likely affects the localization of lipidated proteins in general.
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Jannik Bruun
authors.initials: JB
authors.lastname: Larsen
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Martin Borch
authors.initials: MB
authors.lastname: Jensen
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Vikram K
authors.initials: VK
authors.lastname: Bhatia
authors.affiliation: 1] Department of Chemistry, University of Copenhagen, Denmark. [2] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Søren L
authors.initials: SL
authors.lastname: Pedersen
authors.affiliation: Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark.
authors.forename: Thomas
authors.initials: T
authors.lastname: Bjørnholm
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Lars
authors.initials: L
authors.lastname: Iversen
authors.affiliation: Department of Chemical Engineering, University of South Carolina, Columbia, South Carolina, USA.
authors.forename: Mark
authors.initials: M
authors.lastname: Uline
authors.affiliation: Department of Biomedical Engineering, Department of Chemistry and Chemistry of Life Processes Institute, Northwestern University, Evanston, Illinois, USA.
authors.forename: Igal
authors.initials: I
authors.lastname: Szleifer
authors.affiliation: 1] Department of Chemistry, University of Copenhagen, Denmark. [2] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Knud J
authors.initials: KJ
authors.lastname: Jensen
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Nikos S
authors.initials: NS
authors.lastname: Hatzakis
authors.affiliation: 1] Bio-Nanotechnology and Nanomedicine Laboratory, Nano-Science Center, University of Copenhagen, Denmark. [2] Department of Chemistry, University of Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Denmark.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Stamou
mesh_terms: D008051:Lipid Bilayers; D008081:Liposomes / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry; D008566:Membranes / Q000737:chemistry* / Q000648:ultrastructure; D020559:Monomeric GTP-Binding Proteins / Q000737:chemistry*; D019308:Palmitic Acid / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D019308:Palmitic Acid; D020559:Monomeric GTP-Binding Proteins; C017251:1,2-oleoylphosphatidylcholine