title: MiR-376a and histone deacetylation 9 form a regulatory circuitry in hepatocellular carcinoma.
abstract: OBJECTIVE
  Our previous study has demonstrated that down-regulation of miR-376a might contribute to the development of hepatocellular carcinoma (HCC), but the mechanism underlying this down-regulation remains obscure.
  
  
  RESULTS
  histone deacetylase (HDAC) inhibitor increased the level of miR-376a in L02 and Huh7 cells by up-regulating the acetylation level of histone 3 at the Maternally expressed 3 (Meg3) differentially methylated region (DMR). Interestingly, HDAC9, a histone deacetylase responsible for deacetylating lysine 18 of histone 3 (H3K18), was identified as the target of miR-376a. In addition, HDAC9 siRNA increased the expression of miR-376a by up-regulating the global histone H3K18 acetylation level, with Meg3 DMR included. Finally, miR-376a and HDAC9 were inversely correlated in HCC.
  
  
  CONCLUSIONS
  HDAC9 plays an important role both as effects and targets of miR-376a.
authors.affiliation: Medical College, Jiaxing University, Jiaxing, China.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Zheng
authors.forename: Huan
authors.initials: H
authors.lastname: Chen
authors.forename: Manxiang
authors.initials: M
authors.lastname: Yin
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Ye
authors.forename: Guiqian
authors.initials: G
authors.lastname: Chen
authors.forename: Xinmei
authors.initials: X
authors.lastname: Zhou
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Chengwen
authors.initials: C
authors.lastname: Zhang
authors.forename: Baoyue
authors.initials: B
authors.lastname: Ding
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D000077209:Decitabine; D015536:Down-Regulation / Q000187:drug effects; D044127:Epigenesis, Genetic; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C573498:MIRN376C microRNA, human; D035683:MicroRNAs; D012097:Repressor Proteins; D000077209:Decitabine; C486544:HDAC9 protein, human; D006655:Histone Deacetylases; D001374:Azacitidine