title: Luteal activity of Abadeh ecotype does in summer and winter and the effect of kisspeptin-10 on luteinizing hormone secretion in the anestrus does.
abstract: The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 goats in summer (n = 6) and winter (n = 4) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P4 concentration ≥1 ng mL(-1), at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P4 measurement 20 and 10 days before the kisspeptin injection in five female Abadeh ecotype goats (4 to 5 years old). The goats were given a single IV injection of saline (2 mL) as control group and the same goats (1 hr after the last blood sampling) were given kisspeptin (1 μg kg(-1)) as treatment group. The blood samples were collected at -60, -40, -20 and 0 min (before injection), and 10, 20, 30, 40, 50, 60, 80, 100, 120 and 140 min after the injection and LH concentration were measured. A single IV injection of 1 µg kg(-1) of kisspeptin-10 did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arjmand
authors.affiliation: Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
authors.forename: Abdolah
authors.initials: A
authors.lastname: Mirzaei
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Jafarzadeh Shirazi
authors.affiliation: Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
authors.forename: Amin
authors.initials: A
authors.lastname: Tamadon
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Mohammad Saied
authors.initials: MS
authors.lastname: Salehi
authors.affiliation: Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;
authors.forename: Mehdi
authors.initials: M
authors.lastname: Saeb
authors.affiliation: Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences and Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Namavar
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zandi
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Hojatollah
authors.initials: H
authors.lastname: Shahheidari
authors.affiliation: Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran;
authors.forename: Sara
authors.initials: S
authors.lastname: Moradi
keywords: Anestrus; Goat; Kisspeptin; Luteal activity; Luteinizing hormone