title: Analysis of gene expression profiles reveals the regulatory network of cold-inducible RNA-binding protein mediating the growth of BHK-21 cells.
abstract: Cold-inducible RNA-binding protein (Cirp), the first cold-shock protein identified in mammals, is a sensor protein whose expression increases in response to stress. Recent reports have shown that Cirp is involved in cell proliferation, development, circadian modulation under physiological conditions, and tumor formation and progression. However, the molecular mechanisms underlying the activities of Cirp in the mammalian kidney cells remain unclear. In this study, we constructed BHK-21cells overexpressing Cirp (Cirp + BHK-21) knockdown BHK-21 cells (Cirp - BHK-21) to investigate the function of Cirp in cell proliferation. We analyzed the gene expression of Cirp - BHK-21 cells using genome-wide expression microarrays to explore the molecular mechanism of Cirp action. We found that (1) Cirp overexpression significantly enhanced cell proliferation, whereas Cirp knockdown dramatically reduced cell proliferation, suggesting that Cirp is a positive regulator of BHK-21 cell proliferation. (2) Differentially expressed genes in Cirp - BHK-21 and control cells were shown to be involved in many biological processes. (3) Pathway analysis showed that five enriched pathways, namely, Focal adhesion, Mapk, Wnt, Apoptosis, and Cancer-related signaling pathways, were identified as central pathway networks regulated by Cirp. These results can provide new insights into the molecular mechanisms of Cirp function.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Tang
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Yuanwei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Daoliang
authors.initials: D
authors.lastname: Lan
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Peiting
authors.initials: P
authors.lastname: Nie
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu, 610041, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yue
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D002874:Chromosome Mapping; D006224:Cricetinae; D020869:Gene Expression Profiling; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D051379:Mice; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: C106176:Cirbp protein, mouse; D016601:RNA-Binding Proteins
keywords: BHK21 cell; cell division; cold-inducible RNA-binding protein; gene expression; regulatory network