title: Production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde by recombinant Escherichia coli co-expressing Lactobacillus reuteri propanediol utilization enzymes.
abstract: 3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from 3-HPA. 3-HP was the sole product with resting cells, while growing cells produced 1,3-propanediol as co-product. 3-HP production from glycerol was achieved with a yield of 0.68mol/mol by feeding recombinant E. coli with 3-HPA produced by L. reuteri and recovered using bisulfite-functionalized resin.
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden. Electronic address: ramin.sabet_azad@biotek.lu.se.
authors.forename: Ramin
authors.initials: R
authors.lastname: Sabet-Azad
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Roya R R
authors.initials: RR
authors.lastname: Sardari
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Javier A
authors.initials: JA
authors.lastname: Linares-Pastén
authors.affiliation: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
authors.forename: Rajni
authors.initials: R
authors.lastname: Hatti-Kaul
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000449:Aldehyde Reductase / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005985:Glyceraldehyde / Q000031:analogs & derivatives* / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D019344:Lactic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D030781:Organisms, Genetically Modified; D010709:Phosphate Acetyltransferase / Q000235:genetics / Q000378:metabolism; D017851:Phosphotransferases (Carboxyl Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011407:Propane / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins
chemical_list: D000445:Aldehyde Oxidoreductases; D000449:Aldehyde Reductase; D029968:Escherichia coli Proteins; D005985:Glyceraldehyde; D005990:Glycerol; D019344:Lactic Acid; D010709:Phosphate Acetyltransferase; D017851:Phosphotransferases (Carboxyl Group Acceptor); D011407:Propane; D011994:Recombinant Proteins; C047158:3-hydroxypropionaldehyde; C031601:hydracrylic acid; C528732:YqhD protein, E coli; C588134:coenzyme A-acylating propionaldehyde dehydrogenase, Lactobacillus reuteri; D001426:Bacterial Proteins; C111066:TdcD protein, E coli
keywords: 3-Hydroxypropionic acid; Lactobacillus reuteri; Propanediol utilization pathway; Recombinant Escherichia coli; Two-step biotransformation