title: Evaluation of a dansyl-based amino acid DNSBA as an imaging probe for apoptosis detection.
abstract: Imaging agents that enable direct detection of apoptosis are highly desirable in the field of monitoring chemotherapeutic response as well as early diagnosis and disease monitoring. Previous work demonstrated that the dansyled amino acid DNSBA is used to specifically and selectively detect apoptotic cancer cells at the both early and late stages, but the mechanism remains unclear. In this work, we evaluated DNSBA as a tool for monitoring cell apoptosis in CNE1 tumor cell models both in vitro and ex vivo after its in vivo administration, which was confirmed by other assays. The ability of DNSBA to detect multiple pathways and different stages of apoptosis leading to cell death may be advantageous in the evaluation of cancer treatment indicative of a positive therapeutic outcome. The uptake change of molecular probes DNSBA in CNE1 cells represented the changes of apoptotic rate in a caspase-dependent manner. However, the accumulation of DNSBA in apoptotic cells did not increase with the enhanced membrane permeability. Furthermore, ex vivo study demonstrated DNSBA has a similar pattern as the TUNEL-positive cells. In conclusion, DNSBA cellular imaging is useful for the early assessment of treatment-induced apoptosis, and thus may act as a substitute for Annexin V for assessing treatment response.
authors.affiliation: Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha, 410078, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Huang
authors.forename: Xinxian
authors.initials: X
authors.lastname: Weng
authors.forename: Lifang
authors.initials: L
authors.lastname: Yang
authors.forename: Meihui
authors.initials: M
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Wang
authors.forename: Jinghe
authors.initials: J
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.forename: Wenbin
authors.initials: W
authors.lastname: Zeng
authors.forename: Lunquan
authors.initials: L
authors.lastname: Sun
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D017304:Annexin A5 / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D001692:Biological Transport; D002277:Carcinoma; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002463:Cell Membrane Permeability; D003619:Dansyl Compounds / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D020287:In Situ Nick-End Labeling; D007279:Injections, Subcutaneous; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D015335:Molecular Probes / Q000737:chemistry / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009368:Neoplasm Transplantation; D061848:Optical Imaging / Q000379:methods*; D017239:Paclitaxel / Q000494:pharmacology; D010666:Phenylpropionates / Q000737:chemistry / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: C551923:4-(5-dimethylaminonaphthalene-1-sulfonamido)-3-(4-iodophenyl)butanoic acid; D017304:Annexin A5; D003619:Dansyl Compounds; D015335:Molecular Probes; D010666:Phenylpropionates; D014409:Tumor Necrosis Factor-alpha; D020169:Caspases; D017239:Paclitaxel