title: Does rat fetal DNA induce preeclampsia in pregnant rats?
abstract: Cell-free fetal DNA in maternal circulation is higher during preeclampsia. It is unclear whether it is the cause or the consequence of the disease. The aim of this study was to prove whether injected rat fetal DNA induces preeclampsia-like symptoms in pregnant Wistar rats. They received daily i.p. injections of water or rat fetal DNA (400 μg) from gestation day 14 to 18. Blood pressure, proteinuria, placental and fetal weight were measured at gestation day 19. Plasma DNase activity, proteinuria and creatinine clearance were assessed. There was no significant difference in any of the measured parameters. The results of this study do not confirm the hypothesis that fetal DNA might induce preeclampsia. This is in contrast to others using human fetal DNA in mice. Further studies should be focused on the effects of fetal DNA from the same species protected from DNase activity.
authors.affiliation: 1Institute of Molecular Biomedicine, Faculty of Medicine,Comenius University,Bratislava,Slovakia.
authors.forename: B
authors.initials: B
authors.lastname: Konečná
authors.affiliation: 1Institute of Molecular Biomedicine, Faculty of Medicine,Comenius University,Bratislava,Slovakia.
authors.forename: V
authors.initials: V
authors.lastname: Borbélyová
authors.affiliation: 1Institute of Molecular Biomedicine, Faculty of Medicine,Comenius University,Bratislava,Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Celec
authors.affiliation: 1Institute of Molecular Biomedicine, Faculty of Medicine,Comenius University,Bratislava,Slovakia.
authors.forename: B
authors.initials: B
authors.lastname: Vlková
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood / Q000652:urine; D004247:DNA / Q000302:isolation & purification / Q000633:toxicity*; D003851:Deoxyribonucleases / Q000097:blood; D005260:Female; D005333:Fetus; D007113:Immunity, Innate / Q000235:genetics; D008180:Lupus Erythematosus, Systemic / Q000150:complications; D011225:Pre-Eclampsia / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D011247:Pregnancy; D011507:Proteinuria / Q000652:urine; D051381:Rats
chemical_list: D004247:DNA; D003404:Creatinine; D003851:Deoxyribonucleases
keywords: extracellular DNA; fetal DNA; innate immunity; preeclampsia