title: [Application of hepatocellular carcinoma targeted multifunctional nanocarriers].
abstract: OBJECTIVE
  To synthesize the RGD-modified magnetic resonance imaging (MRI)-visible gene transfer nanocarrier and assess its gene delivery ability and MRI visibility for hepatocellular carcinoma.
  
  
  METHODS
  The multifunctional nanocarrier RGD-PEG-PEI-SPION was constructed. And the degree of binding between nanocarrier and siRNA was determined by agarose gel electrophoresis. The zeta potential and particle size of cationic polymer vectors were measured with a Zeta-Plus instrument. Immunocytochemical assay was performed for detecting the expression of α(v)β(3) in Bel-7402 cells. The active targeting ability of nanocarrier to Bel-7402 cells was evaluated by flow cytometry and laser confocal microcopy. The MRI visibility of nanocarrier to Bel-7402 cells was assessed.
  
  
  RESULTS
  RGD-PEG-PEI-SPION could condense siRNA entirely at a N/P ratio of 2.8; the membranes of Bel-7402 cells possessed an enrichment of α(v)β(3) receptors. At a nitrogen/phosphate ratio of 10, the particle size of RGD-PEG-PEI-SPION/siRNA attained a constant size of 85.2 ± 5.6 nm, the zeta potential reached +12.4 ± 1.2 mV, the gene transfection efficiency of nanocarrier to Bel-7402 cells attained 71.2% ± 2.1% and the cells showed significantly stronger RGD-PEG-PEI-SPION (red) and siRNA (green) fluorescence under laser confocal microcopy. The cells incubated with RGD-PEG-PEI-SPION exhibited significantly lower normalized MR T(2)(*)WI signal intensity.
  
  
  CONCLUSIONS
  A RGD-modified MRI-visible gene delivery nanocarrier RGD-PEG-PEI-SPION has been successfully synthesized.It has a higher transfection efficiency of transferring siRNA into Bel-7402 cells and could sensitively detect hepatocellular carcinoma with MRI.
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Linglan
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Zaibo
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Kangshun
authors.initials: K
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China.
authors.forename: Shouhai
authors.initials: S
authors.lastname: Guan
authors.affiliation: Department of Radiology, Third Affiliated Hospital; Insitute of Interventional Radiology, Sun Yat-sen University, Guangzhou 510630, China. Email: shanhong@mail.sysu.edu.cn.
authors.forename: Hong
authors.initials: H
authors.lastname: Shan
mesh_terms: D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008113:Liver Neoplasms; D008279:Magnetic Resonance Imaging; D053758:Nanoparticles / Q000008:administration & dosage*; D010316:Particle Size; D011092:Polyethylene Glycols; D011094:Polyethyleneimine / Q000031:analogs & derivatives; D034741:RNA, Small Interfering; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); D011092:Polyethylene Glycols; D011094:Polyethyleneimine