title: The centrosomal component CEP161 of Dictyostelium discoideum interacts with the Hippo signaling pathway.
abstract: CEP161 is a novel component of the Dictyostelium discoideum centrosome which was identified as binding partner of the pericentriolar component CP250. Here we show that the amino acids 1-763 of the 1381 amino acids CEP161 are sufficient for CP250 binding, centrosomal targeting and centrosome association. Analysis of AX2 cells over-expressing truncated and full length CEP161 proteins revealed defects in growth and development. By immunoprecipitation experiments we identified the Hippo related kinase SvkA (Hrk-svk) as binding partner for CEP161. Both proteins colocalize at the centrosome. In in vitro kinase assays the N-terminal domain of CEP161 (residues 1-763) inhibited the kinase activity of Hrk-svk. A comparison of D. discoideum Hippo kinase mutants with mutants overexpressing CEP161 polypeptides revealed similar defects. We propose that the centrosomal component CEP161 is a novel player in the Hippo signaling pathway and affects various cellular properties through this interaction.
authors.affiliation: a Institute of Biochemistry I; Medical Faculty University of Cologne ; Köln , Germany.
authors.forename: Salil K
authors.initials: SK
authors.lastname: Sukumaran
authors.forename: Rosemarie
authors.initials: R
authors.lastname: Blau-Wasser
authors.forename: Meino
authors.initials: M
authors.lastname: Rohlfs
authors.forename: Christoph
authors.initials: C
authors.lastname: Gallinger
authors.forename: Michael
authors.initials: M
authors.lastname: Schleicher
authors.forename: Angelika A
authors.initials: AA
authors.lastname: Noegel
mesh_terms: D000595:Amino Acid Sequence; D002478:Cells, Cultured; D018385:Centrosome / Q000378:metabolism*; D004023:Dictyostelium / Q000378:metabolism*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D015800:Protozoan Proteins; D017346:Protein Serine-Threonine Kinases
keywords: CEP161; Dictyostelium discoideum; Hippo signaling; centrosome; hippo kinase