title: Screening peptide array library for the identification of cancer cell-binding peptides.
abstract: The identification of cancer cell-specific ligands is a key requirement for the targeted delivery of chemotherapeutic agents. Usually phage display system is employed to discover cancer-specific peptides through a biopanning process. Synthetic peptide array libraries can be used as a complementary method to phage display for screening and identifying cancer cell-specific ligands. Here, we describe a peptide array-whole cell binding assay to identify cancer cell-specific peptides. A peptide array library based on a lead dodecapeptide, p160, is synthesized on a functionalized cellulose membrane using solid phase chemistry and a robotic synthesizer. The relative binding affinity of the peptide library is evaluated by incubating the library with fluorescently labeled cancerous or non-cancerous cells. Thereby the assay allows picking peptides that show selective and high binding to cancerous cells. These peptides represent potential candidates for use in cancer-targeted drug delivery, imaging, and diagnosis.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, 2-142K Katz Group-Rexall Centre for Pharmacy & Health Research, University of Alberta, 11361-87 Ave, Edmonton, AB, Canada, T6G 2E1, kkaur@ualberta.ca.
authors.forename: Kamaljit
authors.initials: K
authors.lastname: Kaur
authors.forename: Sahar
authors.initials: S
authors.lastname: Ahmed
authors.forename: Rania
authors.initials: R
authors.lastname: Soudy
authors.forename: Sarfuddin
authors.initials: S
authors.lastname: Azmi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D019151:Peptide Library; D011485:Protein Binding
chemical_list: D009842:Oligopeptides; D019151:Peptide Library