title: Reproduction potentiated in nematodes (Caenorhabditis elegans) and guppy fish (Poecilia reticulata) by adding a synthetic peptide to their aqueous environment.
abstract: Ambient exposure to a short synthetic peptide has enhanced fecundity (number of offspring) in invertebrates and vertebrates, ostensibly by disinhibiting reproduction. In separate experiments, nematodes (Caenorhabditis elegans) and guppy fish (Poecilia reticulata) were exposed via their aqueous environment to a dissolved synthetic hexamer (6mer) peptide, IEPVFT (EPL036), at a concentration of 1 μmol l(-1). In the case of the worms, peptide was added to their aqueous buffer daily throughout the experiment (14 days); for the guppies, peptide administration was on the first 15 alternate days in a 50 week experiment. Fecundity rose by 79% among the worms. The number of descendants of the treated guppies was more than four times that of controls by week 26 (103 versus 25, including 72 juveniles versus 6), with 15.4% more estimated biomass in the test tank in total (i.e. including founders). It was deduced that treated females bred earlier, at a smaller size, and had larger brood sizes. The total number of fish in the control tank had caught up by termination, but biomass continued to lag the test tank. There were no overt signs of toxicity among either the worms or the fish. Bioinformatics has been unilluminating in explaining these results in terms, for example, of mimicry of an endogenous regulator. A mass spectrometric campaign to identify a receptor, using murine brain for expediency, proved inconclusive. Molecular modelling in silico indicated unexpectedly that the hexamer EPL036 might be acting as an antagonist, to pro-fecundity effect; that is, as a blocker of an inhibitor. This suggests that there awaits discovery an evolutionarily conserved reproductive inhibitor and its (anti-fecundity) receptor.
authors.affiliation: School of Life and Medical Sciences, University of Hertfordshire, College Lane, Hatfield AL10 9AB, UK k.davies@herts.ac.uk.
authors.forename: Keith G
authors.initials: KG
authors.lastname: Davies
authors.affiliation: Aquarium, Zoological Society of London (ZSL), Regent's Park, London NW1 4RY, UK.
authors.forename: Brian
authors.initials: B
authors.lastname: Zimmerman
authors.affiliation: Biochemistry Group, Institute of Life Sciences, Medical School, Swansea University, Swansea SA2 8PP, UK.
authors.forename: Ed
authors.initials: E
authors.lastname: Dudley
authors.affiliation: Biochemistry Group, Institute of Life Sciences, Medical School, Swansea University, Swansea SA2 8PP, UK.
authors.forename: Russell P
authors.initials: RP
authors.lastname: Newton
authors.affiliation: Endocrine Pharmaceuticals Ltd, Wilderness End, Tadley Common Road, Tadley, Hampshire RG26 3TA, UK.
authors.forename: John E
authors.initials: JE
authors.lastname: Hart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018533:Biomass; D001921:Brain / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000502:physiology*; D003198:Computer Simulation; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology*; D011036:Poecilia / Q000502:physiology*; D018000:Receptors, Peptide / Q000378:metabolism; D012098:Reproduction; D013045:Species Specificity
chemical_list: D009842:Oligopeptides; D018000:Receptors, Peptide
keywords: Aphrodisiac; Breeding; Fecundity; Fertility; Infertility; Reproductive stimulator