title: Red fluorescent zinc oxide nanoparticle: a novel platform for cancer targeting.
abstract: Multifunctional zinc oxide (ZnO) nanoparticles (NPs) with well-integrated multimodality imaging capacities have generated increasing research interest in the past decade. However, limited progress has been made in developing ZnO NP-based multimodality tumor-imaging agents. Here we developed novel red fluorescent ZnO NPs and described the successful conjugation of 64Cu (t1/2=12.7 h) and TRC105, a chimeric monoclonal antibody against CD105, to these ZnO NPs via well-developed surface engineering procedures. The produced dual-modality ZnO NPs were readily applicable for positron emission tomography (PET) imaging and fluorescence imaging of the tumor vasculature. Their pharmacokinetics and tumor-targeting efficacy/specificity in mice bearing murine breast 4T1 tumor were thoroughly investigated. ZnO NPs with dual-modality imaging properties can serve as an attractive candidate for future cancer theranostics.
authors.affiliation: Department of Radiology, University of Wisconsin-Madison , Madison, Wisconsin 53705-2275, United States.
authors.forename: Hao
authors.initials: H
authors.lastname: Hong
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Graves
authors.forename: Savo Bou Zein
authors.initials: SB
authors.lastname: Eddine
authors.forename: Yunan
authors.initials: Y
authors.lastname: Yang
authors.forename: Charles P
authors.initials: CP
authors.lastname: Theuer
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Nickles
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
authors.forename: Weibo
authors.initials: W
authors.lastname: Cai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000493:pharmacokinetics; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D003301:Copper Radioisotopes / Q000493:pharmacokinetics; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods; D064847:Multimodal Imaging / Q000379:methods*; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015034:Zinc Oxide
chemical_list: D000911:Antibodies, Monoclonal; D003301:Copper Radioisotopes; D005456:Fluorescent Dyes; D053769:Nanocapsules; D019275:Radiopharmaceuticals; D015034:Zinc Oxide; C579557:carotuximab
keywords: CD105; angiogenesis; drug delivery; imaging; positron emission tomography; zinc oxide