title: DENND1A gene variants in Bahraini Arab women with polycystic ovary syndrome.
abstract: Recent genome-wide association studies and replication analyses reported an association between variants of DENND1A gene and polycystic ovary syndrome (PCOS), mostly in Asians. We therefore examined whether the common DENND1A SNPs rs10818854, rs2479106, and rs10986105 are associated with PCOS in Bahraini Arab population. This case-control study involved 191 women with PCOS diagnosed according to the Rotterdam criteria, and 202 control women. SNP genotyping was performed by the allelic discrimination in real-time PCR. The outcome was that the minor allele frequencies of SNPs rs10818854, rs2479106, and rs10986105 were similar between women with PCOS and control women (P>0.05), even before correcting for multiple testing, and none of the tested DENND1A SNPs were associated with PCOS under co-dominant, dominant, or recessive genetic models. None of the tested DENND1A variants were associated with PCOS features (hirsutism, insulin sensitivity, menses pattern, free testosterone, and free androgen index). Taking common GTA haplotype as reference (OR=1.00), [rs10818854/rs2479106/rs10986105] 3-locus haplotype analysis demonstrated lack of association of any of the DENND1A haplotypes with PCOS, even before correcting for multiple testing. To conclude we demonstrated lack of association of DENND1A SNPs rs10818854, rs2479106, and rs10986105, previously associated with PCOS in Asians, with PCOS in Bahraini Arab women.
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Emily
authors.initials: E
authors.lastname: Gammoh
authors.affiliation: Department of Internal Medicine, Arabian Gulf University, Manama, Bahrain.
authors.forename: Mona R
authors.initials: MR
authors.lastname: Arekat
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: F Lisa
authors.initials: FL
authors.lastname: Saldhana
authors.affiliation: Department of Obstetrics and Gynecology, Salmaniya Medical Complex, Manama, Bahrain.
authors.forename: Samira
authors.initials: S
authors.lastname: Madan
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain.
authors.forename: Bashayer H
authors.initials: BH
authors.lastname: Ebrahim
authors.affiliation: Department of Medical Biochemistry, Arabian Gulf University, Manama, Bahrain. Electronic address: wassim@agu.edu.bh.
authors.forename: Wassim Y
authors.initials: WY
authors.lastname: Almawi
mesh_terms: D000328:Adult; D018912:Arabs / Q000235:genetics*; D001445:Bahrain / Q000453:epidemiology; D016022:Case-Control Studies; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D011085:Polycystic Ovary Syndrome / Q000453:epidemiology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D055815:Young Adult
chemical_list: C571984:DENND1A protein, human; D053418:Death Domain Receptor Signaling Adaptor Proteins; D020662:Guanine Nucleotide Exchange Factors
keywords: DENND1A variants; Haplotypes; Polycystic ovary syndrome; SNPs