title: A ratiometric fluorescent probe for gasotransmitter hydrogen sulfide based on a coumarin-benzopyrylium platform.
abstract: A ratiometric fluorescent probe for H2S was developed based on a coumarin- benzopyrylium platform. The ratiometric sensing is realized by a selective conversion of acyl azide to the corresponding amide, which subsequently undergoes an intramolecular spirocyclization to alter the large π-conjugated system of CB fluorophore. Compared with the traditional azide-based H2S probes, the proposed probe utilizes the acyl azide as the recognition moiety and exhibits a rapid response (∼1min) towards H2S, which is superior to most of the azide-based H2S probes. Preliminary fluorescence imaging experiments show that probe 1 has potential to track H2S in living cells.
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Duan
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, PR China. Electronic address: xfyang@nwu.edu.cn.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Yaogang
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, PR China. Electronic address: guoyuan@nwu.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Life Sciences, Northwest University, Xi'an 710069, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
mesh_terms: D001578:Benzopyrans / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D003374:Coumarins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D064426:Gasotransmitters / Q000032:analysis*; D055166:Hepatic Stellate Cells / Q000737:chemistry / Q000166:cytology; D006801:Humans; D006862:Hydrogen Sulfide / Q000032:analysis*; D061848:Optical Imaging
chemical_list: D001578:Benzopyrans; D003374:Coumarins; D005456:Fluorescent Dyes; D064426:Gasotransmitters; C030123:coumarin; D006862:Hydrogen Sulfide
keywords: Acyl azide; Benzopyrylium; Hydrogen sulfide; Ratiometric; Spirocyclization