title: Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa.
abstract: Inflammatory bowel diseases are chronic gastrointestinal pathologies causing great discomfort in both children and adults. The pathogenesis of inflammatory bowel diseases is not yet fully understood and their diagnosis and treatment are often challenging. Nanoparticle-based strategies have been tested in local drug delivery to the inflamed colon. Here, we have investigated the use of the novel avidin-nucleic acid nanoassembly (ANANAS) platform as a potential diagnostic carrier in an experimental model of inflammatory bowel diseases. Fluorescent- labeled ANANAS nanoparticles were administered to mice with chemically induced chronic inflammation of the large intestine. Localization of mucosal nanoparticles was assessed in vivo by dual-band confocal laser endomicroscopy. This technique enables characterization of the mucosal microvasculature and crypt architecture at subcellular resolution. Intravascular nanoparticle distribution was observed in the inflamed mucosa but not in healthy controls, demonstrating the utility of the combination of ANANAS and confocal laser endomicroscopy for highlighting intestinal inflammatory conditions. The specific localization of ANANAS in inflamed tissues supports the potential of this platform as a targeted carrier for bioactive moieties in the treatment of inflammatory bowel disease.
authors.affiliation: Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, Padova, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Buda
authors.affiliation: Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, Padova, Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Facchin
authors.affiliation: Department of Molecular Medicine, University of Padova, Padova, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Dassie
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Casarin
authors.affiliation: School of Biochemistry and Wolfson Bioimaging Facility, University of Bristol, Bristol, UK.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Jepson
authors.affiliation: Ludwig Demlig Endoscopic Center of Excellence, ESGE Endoscopy Training Center, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Neumann
authors.affiliation: Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, Padova, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Hatem
authors.affiliation: Veneto Institute of Oncology IOV-IRCCS, Padova, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Realdon
authors.affiliation: Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, Padova, Italy.
authors.forename: Renata
authors.initials: R
authors.lastname: D'Incà
authors.affiliation: Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, Padova, Italy.
authors.forename: Giacomo Carlo
authors.initials: GC
authors.lastname: Sturniolo
authors.affiliation: Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Morpurgo
mesh_terms: D000818:Animals; D001360:Avidin / Q000032:analysis / Q000737:chemistry; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D015212:Inflammatory Bowel Diseases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D055806:Microvessels / Q000737:chemistry*; D053758:Nanoparticles / Q000032:analysis / Q000737:chemistry; D009696:Nucleic Acids / Q000032:analysis / Q000737:chemistry
chemical_list: D005456:Fluorescent Dyes; D009696:Nucleic Acids; D001360:Avidin
keywords: avidin-nucleic acid nanoassembly; confocal laser endomicroscopy; dextran sodium sulfate; diagnostics; fluorescent nanoparticles; inflammatory bowel disease; ulcerative colitis