title: Non-invasive in vivo characterization of human carotid plaques with acoustic radiation force impulse ultrasound: comparison with histology after endarterectomy.
abstract: Ischemic stroke from thromboembolic sources is linked to carotid artery atherosclerotic disease with a trend toward medical management in asymptomatic patients. Extent of disease is currently diagnosed by non-invasive imaging techniques that measure luminal stenosis, but it has been suggested that a better biomarker for determining risk of future thromboembolic events is plaque morphology and composition. Specifically, plaques that are composed of mechanically soft lipid/necrotic regions covered by thin fibrous caps are the most vulnerable to rupture. An ultrasound technique that non-invasively interrogates the mechanical properties of soft tissue, called acoustic radiation force impulse (ARFI) imaging, has been developed as a new modality for atherosclerotic plaque characterization using phantoms and atherosclerotic pigs, but the technique has yet to be validated in vivo in humans. In this preliminary study, in vivo ARFI imaging is presented in a case study format for four patients undergoing clinically indicated carotid endarterectomy and compared with histology. In two type Va plaques, characterized by lipid/necrotic cores covered by fibrous caps, mean ARFI displacements in focal regions were high relative to the surrounding plaque material, suggesting soft features were covered by stiffer layers within the plaques. In two type Vb plaques, characterized by heavy calcification, mean ARFI peak displacements were low relative to the surrounding plaque and arterial wall, suggesting stiff tissue. This pilot study illustrates the feasibility and challenges of transcutaneous ARFI for characterizing the material and structural composition of carotid atherosclerotic plaques via mechanical properties, in humans, in vivo.
authors.affiliation: Joint Department of Biomedical Engineering, University of North Carolina and North Carolina State University, Chapel Hill, North Carolina, USA.
authors.forename: Tomasz J
authors.initials: TJ
authors.lastname: Czernuszewicz
authors.affiliation: Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA; McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Jonathon W
authors.initials: JW
authors.lastname: Homeister
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Melissa C
authors.initials: MC
authors.lastname: Caughey
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Farber
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Fulton
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Ford
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Marston
authors.affiliation: Department of Surgery, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Raghuveer
authors.initials: R
authors.lastname: Vallabhaneni
authors.affiliation: Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina, USA; Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA.
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Nichols
authors.affiliation: McAllister Heart Institute, University of North Carolina, Chapel Hill, North Carolina, USA; Department of Electrical and Computer Engineering, North Carolina State University, Raleigh, North Carolina, USA; Biomedical Research Imaging Center, University of North Carolina, Chapel Hill, North Carolina, USA. Electronic address: cmgallip@bme.unc.edu.
authors.forename: Caterina M
authors.initials: CM
authors.lastname: Gallippi
mesh_terms: D000368:Aged; D002343:Carotid Artery, Internal / Q000000981:diagnostic imaging*; D016893:Carotid Stenosis / Q000000981:diagnostic imaging* / Q000601:surgery*; D054459:Elasticity Imaging Techniques / Q000379:methods*; D016894:Endarterectomy, Carotid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000000981:diagnostic imaging*; D011237:Predictive Value of Tests; D018570:Risk Assessment; D012307:Risk Factors; D020521:Stroke / Q000517:prevention & control; D016896:Treatment Outcome
keywords: Acoustic radiation force impulse; Atherosclerosis; Carotid endarterectomy; Plaque characterization; Stroke; Ultrasound