title: Identification of a novel human rhinovirus C type by antibody capture VIDISCA-454.
abstract: Causative agents for more than 30 percent of respiratory infections remain unidentified, suggesting that unknown respiratory pathogens might be involved. In this study, antibody capture VIDISCA-454 (virus discovery cDNA-AFLP combined with Roche 454 high-throughput sequencing) resulted in the discovery of a novel type of rhinovirus C (RV-C). The virus has an RNA genome of at least 7054 nt and carries the characteristics of rhinovirus C species. The gene encoding viral protein 1, which is used for typing, has only 81% nucleotide sequence identity with the closest known RV-C type, and, therefore, the virus represents the first member of a novel type, named RV-C54.
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. s.m.jazaerifarsani@amc.uva.nl.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Jazaeri Farsani
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. b.b.oudemunnink@amc.uva.nl.
authors.forename: Bas B
authors.initials: BB
authors.lastname: Oude Munnink
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. marta.canuti@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Canuti
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. m.deijs@amc.uva.nl.
authors.forename: Martin
authors.initials: M
authors.lastname: Deijs
authors.affiliation: Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK. mc13@sanger.ac.uk.
authors.forename: Matthew
authors.initials: M
authors.lastname: Cotten
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. m.f.jebbink@amc.uva.nl.
authors.forename: Maarten F
authors.initials: MF
authors.lastname: Jebbink
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. verhoevenjtp@googlemail.com.
authors.forename: Joost
authors.initials: J
authors.lastname: Verhoeven
authors.affiliation: Department of Virus Genomics, Wellcome Trust Sanger Institute, Hinxton CB10 1SA, UK. pk5@sanger.ac.uk.
authors.forename: Paul
authors.initials: P
authors.lastname: Kellam
authors.affiliation: Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. katherine.loens@uza.be.
authors.forename: Katherine
authors.initials: K
authors.lastname: Loens
authors.affiliation: Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. herman.goossens@uza.be.
authors.forename: Herman
authors.initials: H
authors.lastname: Goossens
authors.affiliation: Department of Medical Microbiology, Vaccine and Infectious Disease Institute, University Hospital, Antwerp B-2650, Belgium. greet.ieven@uza.be.
authors.forename: Margareta
authors.initials: M
authors.lastname: Ieven
authors.affiliation: Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center of the University of Amsterdam, Amsterdam 1105 AZ, the Netherlands. c.m.vanderhoek@amc.uva.nl.
authors.forename: Lia
authors.initials: L
authors.lastname: van der Hoek
mesh_terms: D000328:Adult; D000914:Antibodies, Viral; D016000:Cluster Analysis; D005260:Female; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D010850:Picornaviridae Infections / Q000821:virology; D012367:RNA, Viral / Q000235:genetics*; D012137:Respiratory System / Q000821:virology; D012141:Respiratory Tract Infections / Q000821:virology; D012229:Rhinovirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral