title: A pilot evaluation of external quality assessment of GenoType MTBDRplus versions 1 and 2 using dried culture spot material.
abstract: Dried culture spots (DCS) of inactivated Mycobacteria strains designed as part of an external quality assessment (EQA) program for the GeneXpert system has applications to other molecular tuberculosis (TB) diagnostic platforms. DCS tested on the GenoType MTBDRplus and Mycobacterium CM assays performed well with MTBDRplus version 2 but require increased bacterial concentration for use with version 1.
authors.affiliation: Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa natasha.gous@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Gous
authors.affiliation: National Health Laboratory Service, Johannesburg, South Africa.
authors.forename: L E
authors.initials: LE
authors.lastname: Isherwood
authors.affiliation: National Health Laboratory Service, Johannesburg, South Africa.
authors.forename: A
authors.initials: A
authors.lastname: David
authors.affiliation: Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa National Health Laboratory Service, Johannesburg, South Africa.
authors.forename: W
authors.initials: W
authors.lastname: Stevens
authors.affiliation: Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
authors.forename: L E
authors.initials: LE
authors.lastname: Scott
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods* / Q000592:standards; D005838:Genotype; D006801:Humans; D025202:Molecular Diagnostic Techniques; D009161:Mycobacterium / Q000235:genetics* / Q000302:isolation & purification*; D010865:Pilot Projects; D012680:Sensitivity and Specificity; D013045:Species Specificity