title: Determination of tiopronin based on the enhancement of Ru(bpy)₅²⁺ co-reactant electrochemiluminescence.
abstract: It was found that tiopronin could strongly enhance the electrochemiluminescence of tris(2,2'-bipyridine) ruthenium(II) (Ru(bpy)3(2+)) in alkaline solution on a bare Pt electrode, based on which a sensitive, simple and rapid method for the determination of tiopronin was established. Under the optimal conditions, the logarithm of ECL enhancement has a linear relationship with the logarithm of tiopronin concentration in the range from 2.0×10(-7) to 2.0×10(-4) mol L(-1) with a detection limit of 1.0×10(-8) mol L(-1) (S/N= 3), and the relative standard deviation of 1.6% (n=7, c=5.0×10(-6) mol L(-1)). The proposed method has been applied to the determination of tiopronin in pharmaceutical preparations and the results were satisfactory with recoveries of 91.7±1.7%, 98.3±1.0% and 100.8±0.5%, respectively, for three different concentration levels (0.61 μmol L(-1), 6.1 μmol L(-1) and 12.2 μmol L(-1)). According to the study of electrochemical behavior, ECL behavior and ECL emission spectrum of Ru(bpy)3(2+)/tiopronin system, a possible ECL mechanism was proposed.
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fujian, 350108, China; Department of Applied Chemistry, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Dexian
authors.initials: D
authors.lastname: Kong
authors.affiliation: Department of Applied Chemistry, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Applied Chemistry, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Lin
authors.affiliation: Department of Applied Chemistry, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Applied Chemistry, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China. Electronic address: lql3388@126.com.
authors.forename: Qinglu
authors.initials: Q
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fujian, 350108, China. Electronic address: qhw76@fzu.edu.cn.
authors.forename: Qiaohua
authors.initials: Q
authors.lastname: Wei
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fujian, 350108, China.
authors.forename: Yuwu
authors.initials: Y
authors.lastname: Chi
authors.affiliation: MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Institute of Nanomedicine and Nanobiosensing, College of Chemistry, Fuzhou University, Fujian, 350108, China.
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
mesh_terms: D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D049449:Luminescence; D009942:Organometallic Compounds / Q000737:chemistry*; D010984:Platinum; D008625:Tiopronin / Q000032:analysis* / Q000737:chemistry
chemical_list: D009942:Organometallic Compounds; C103599:bis(bipyridyl)ruthenium(II); D010984:Platinum; D015082:2,2'-Dipyridyl; D008625:Tiopronin
keywords: Co-reactant; Electrochemiluminescence; Ru(bpy)(3)(2+); Tiopronin