title: Rhodanobacter koreensis sp. nov., a bacterium isolated from tomato rhizosphere.
abstract: A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated THG-DD7(T), was isolated from tomato plant rhizosphere soil. Strain THG-DD7(T) grew optimally at 25-30 °C, at pH 7.0-7.5 and in the presence of 0.5% (w/v) NaCl. According to the results of 16S rRNA gene sequence comparisons, strain THG-DD7(T) was most closely related to Rhodanobacter umsongensis GR24-2(T) (98.2%), Rhodanobacter panaciterrae LnR5-47(T) (98.0%), Rhodanobacter soli DCY45(T) (97.9%), Rhodanobacter terrae GP18-1(T) (97.9%) and Dyella ginsengisoli Gsoil 3046(T) (97.7%). The DNA G+C content was 65.2 mol%. In DNA-DNA hybridization, the DNA relatedness levels between strain THG-DD7(T) and its closest phylogenetically neighbours were below 40.0%. The predominant isoprenoid quinone was ubiquinone Q-8. The major polar lipids were diphosphatidylglycerol, phosphtidylethanolamine, phosphatidyl-N-methylethanolamine and phosphatidylglycerol. The major fatty acids were iso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0), anteiso-C(15 : 0) and iso-C(17 : 1)ω9c and/or C(16 : 0) 10-methyl (summed feature 9). These data supported the affiliation of strain THG-DD7(T) to the genus Rhodanobacter . The results of physiological and biochemical tests enabled strain THG-DD7(T) to be differentiated genotypically and phenotypically from the species of the genus Rhodanobacter with validly published names. Therefore, the novel isolate represents a novel species, for which the name Rhodanobacter koreensis sp. nov. is proposed. The type strain is THG-DD7(T) ( = KACC 17650(T) = JCM 19614(T)).
authors.affiliation: College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: KyungHwa
authors.initials: K
authors.lastname: Won
authors.affiliation: College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Hina
authors.initials: H
authors.lastname: Singh
authors.affiliation: College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Hien T T
authors.initials: HTT
authors.lastname: Ngo
authors.affiliation: College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: HeungMin
authors.initials: H
authors.lastname: Son
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Genetic Engineering, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Ki-Young
authors.initials: KY
authors.lastname: Kim
authors.affiliation: College of Life science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Gyeonggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D018551:Solanum lycopersicum / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8