title: Morphometric and high resolution scanning electron microscopy analysis of low-level laser therapy and latex protein (Hevea brasiliensis) administration following a crush injury of the sciatic nerve in rats.
abstract: This study evaluated the effect of low-level laser therapy (LLLT; 15 J/cm(2)) and a latex protein (F1) on a crush injury of the sciatic (ischiadicus) nerve. Seventy-two rats (male, 250 g) were divided into 6 groups: CG, control; EG, exposed nerve; IG, injured nerve without treatment; LG, injured nerve with LLLT; HG, injured nerve with F1; and LHG, injured nerve with LLLT and F1. After 4 or 8 weeks, the animals were euthanized and samples of the sciatic nerve were collected for morphometric and high-resolution scanning electron microscopy (HRSEM) analysis. After 4 weeks, the morphometry revealed improvements in the treated animals, and the HG appeared to be the most similar to the CG; after 8 weeks, the injured groups showed improvements compared to the previous period, and the results of the treatment groups were more similar to one another. At HRSEM after 4 weeks, the treated groups were similar and showed improvement compared to the IG; after 8 weeks, the LHG and HG had the best results. In conclusion, the treatments resulted in improvement after the nerve injury, and this recovery was time-dependent. In addition, the use of the F1 resulted in the best morphometric and ultrastructural findings.
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; Ribeirão Preto Medical School of the University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Dias
authors.affiliation: School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto , Brazil.
authors.forename: João Paulo M
authors.initials: JP
authors.lastname: Issa
authors.affiliation: Ribeirão Preto Medical School of the University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Coutinho-Netto
authors.affiliation: Ribeirão Preto Medical School of the University of São Paulo, Ribeirão Preto, Brazil.
authors.forename: Valéria P S
authors.initials: VP
authors.lastname: Fazan
authors.affiliation: School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto , Brazil.
authors.forename: Luiz Gustavo
authors.initials: LG
authors.lastname: Sousa
authors.affiliation: School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto , Brazil.
authors.forename: Mamie M
authors.initials: MM
authors.lastname: Iyomasa
authors.affiliation: School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
authors.forename: Paula C
authors.initials: PC
authors.lastname: Papa
authors.affiliation: Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. Electronic address: watanabe@icb.usp.br.
authors.forename: Ii-Sei
authors.initials: IS
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D028482:Hevea / Q000737:chemistry*; D007840:Latex / Q000008:administration & dosage* / Q000737:chemistry; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009409:Nerve Crush / Q000009:adverse effects; D008517:Phytotherapy; D028321:Plant Preparations; D010940:Plant Proteins / Q000008:administration & dosage / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000187:drug effects / Q000293:injuries* / Q000528:radiation effects / Q000648:ultrastructure; D020426:Sciatic Neuropathy / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D013997:Time Factors
chemical_list: D007840:Latex; D028321:Plant Preparations; D010940:Plant Proteins
keywords: High-resolution scanning electron microscopy; Latex protein — F1; Low-level laser therapy; Morphometric; Nerve regeneration; Ultrastructure