title: Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae.
abstract: The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using tandem mass spectrometry in a global proteome study of hyphae and germinating cysts in P. pisi and P. sojae. In total 2775 proteins from P. pisi and 2891 proteins from P. sojae were identified. Fifty-eight orthologous proteins were more abundant in germinated cysts of both pathogens and thus identified as candidate proteins for the infective stage. Several of these proteins were associated with lipid transport and metabolism, and energy production. Twenty-three orthologous proteins were more abundant in hyphae of both pathogens and thus identified as candidate proteins for vegetative growth. Proteins uniquely present in germinating cysts of either P. pisi or P. sojae were considered as candidates for species-specific pathogenicity factors that may be involved in host specificity. Among these proteins were serine proteases, membrane transporters and a berberine-like protein. These results significantly expand the knowledge of the expressed proteome in P. pisi and P. sojae.
  
  
  CONCLUSIONS
  P. sojae and P. pisi are closely related species that specifically cause root rot on soybean and pea, respectively. The pathogenicity factors contributing to their host specificity remained unknown. We carried out a comparative large-scale proteome analysis of vegetative (hyphae) and infective (germinating cysts) life stages in P. pisi and P. sojae. This study provides knowledge of the common factors and mechanism involved in initiation of infection and species-specific proteins that may contribute to the host specificity of these pathogens. This knowledge will lead to a better understanding of the infection biology of these pathogens, allowing new possibilities towards developing alternative and effective plant protection measures.
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden. Electronic address: Sara.Hosseini@slu.se.
authors.forename: S
authors.initials: S
authors.lastname: Hosseini
authors.affiliation: Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102, SE-23053 Alnarp, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Resjö
authors.affiliation: BGI-Shenzhen, Main Building 11/F, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Durling
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Heyman
authors.affiliation: Bioinformatics Infrastructure for Life Sciences, Department of Immunotechnology, Lund University, Medicon Village, 22381 Lund, Sweden.
authors.forename: F
authors.initials: F
authors.lastname: Levander
authors.affiliation: BGI-Shenzhen, Main Building 11/F, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China.
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Elfstrand
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden.
authors.forename: D
authors.initials: D
authors.lastname: Funck Jensen
authors.affiliation: Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102, SE-23053 Alnarp, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Andreasson
authors.affiliation: Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7026, SE-75007 Uppsala, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Karlsson
mesh_terms: D010838:Phytophthora / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
chemical_list: D020543:Proteome
keywords: Effector; Germinated cyst; Lipid catabolism; Pathogenicity factors; Proteome; Vegetative growth