title: Growth suppression and cell death by pyridoxal is dependent on p53 in the human breast cancer cell line MCF-7.
abstract: Vitamin B6 compound, pyridoxine (PN), has shown antitumor action. Our previous experiments showed that PN induces expression of insulin-like growth factor binding protein-3 to arrest proliferation and induce cell death. This induction is inhibited by the p53-specific inhibitor pifithrin-α. Here, we report that another B6 compound, pyridoxal (PL), strongly inhibited MCF-7 cell growth compared to PN. PL induced the G0/G1 arrest and the accumulation of subG1 population. Although p53 mRNA was not changed by PL, 0.5 mM PL increased the protein level in MCF-7 cells. The cell growth suppression by 0.5 mM PL did not occur when p53 expression was knocked down using siRNA. Together, these data suggest that PL accumulate p53 and PL-induced cell growth suppression is dependent on p53 in MCF-7 breast cancer cells.
authors.affiliation: a Joint Faculty of Veterinary Medicine, Department of Veterinary Pathobiology , Kagoshima University , Kagoshima , Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Minamino
authors.forename: Tatsuzo
authors.initials: T
authors.lastname: Oka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kanouchi
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000235:genetics / Q000378:metabolism; D061986:MCF-7 Cells; D011730:Pyridoxal / Q000494:pharmacology*; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014803:Vitamin B Complex / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; C515497:IGFBP3 protein, human; D018972:Insulin-Like Growth Factor Binding Protein 3; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D016159:Tumor Suppressor Protein p53; D014803:Vitamin B Complex; D011730:Pyridoxal
keywords: MCF-7; insulin-like growth factor binding protein-3 (IGFBP-3); p53; pyridoxal; vitamin B6