title: Protein behavior at surfaces: orientation, conformational transitions and transport.
abstract: Chromatography is the key technology in protein purification as well as in protein refolding. Taking the scientific development and technological innovation of protein chromatography as the objective, this article is devoted to an overview of protein behavior at chromatographic surfaces, including protein orientation, conformational transitions (unfolding and refolding), and protein transport. Recent advances achieved by using molecular simulations as well as theoretical and experimental investigations are elaborated and discussed with emphasis on their implications to the rational design of novel chromatographic surfaces or materials and mobile phase conditions for the development of high-performance protein chromatography.
authors.affiliation: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Linling
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biochemical Engineering and Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China. Electronic address: ysun@tju.edu.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D002845:Chromatography / Q000639:trends*; D011487:Protein Conformation; D058849:Protein Refolding; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: Conformational transition; Mass transport; Orientation; Protein chromatography; Surface behavior; Surface diffusion