title: The Qiagen Investigator® Quantiplex HYres as an alternative kit for DNA quantification.
abstract: The Investigator® Quantiplex HYres kit was evaluated as a potential replacement for dual DNA quantification of casework samples. This kit was determined to be highly sensitive with a limit of quantification and limit of detection of 0.0049ng/μL and 0.0003ng/μL, respectively, for both human and male DNA, using full or half reaction volumes. It was also accurate in assessing the amount of male DNA present in 96 mock and actual casework male:female mixtures (various ratios) processed in this exercise. The close correlation between the male/human DNA ratios expressed in percentages derived from the Investigator® Quantiplex HYres quantification results and the male DNA proportion calculated in mixed AmpFlSTR® Profiler® Plus or AmpFlSTR® Identifiler® Plus profiles, using the Amelogenin Y peak and STR loci, allowed guidelines to be developed to facilitate decisions regarding when to submit samples to Y-STR rather than autosomal STR profiling. The internal control (IC) target was shown to be more sensitive to inhibitors compared to the human and male DNA targets included in the Investigator® Quantiplex HYres kit serving as a good quality assessor of DNA extracts. The new kit met our criteria of enhanced sensitivity, accuracy, consistency, reliability and robustness for casework DNA quantification.
authors.affiliation: Royal Canadian Mounted Police, Forensic Science and Identification Services, 1200 Vanier Parkway, Ottawa, ON K1G 3M8, Canada. Electronic address: chantal.fregeau@rcmp-grc.gc.ca.
authors.forename: Chantal J
authors.initials: CJ
authors.lastname: Frégeau
authors.affiliation: Royal Canadian Mounted Police, Forensic Science and Identification Services, 1200 Vanier Parkway, Ottawa, ON K1G 3M8, Canada.
authors.forename: Nancy
authors.initials: N
authors.lastname: Laurin
mesh_terms: D004247:DNA / Q000032:analysis*; D005260:Female; D006801:Humans; D057230:Limit of Detection; D008297:Male; D015203:Reproducibility of Results
chemical_list: D004247:DNA
keywords: Casework; DNA mixtures; Dual DNA quantification; Forensic science; Inhibition studies; Scorpion primer probe; “Direct to DNA” approach for sexual assault cases