title: A novel ionic liquid-tolerant Fusarium oxysporum BN secreting ionic liquid-stable cellulase: consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid.
abstract: In this study, microbial communities from chemicals polluted microhabitats were cultured with the addition of imidazolium-based ionic liquid (IL) to enrich for IL-tolerant microbes. A strain of Fusarium oxysporum BN producing cellulase from these enrichments was capable of growing in 10% (w/v) 1-ethyl-3-methylimidazolium phosphinate, much higher than the normal IL concentrations in the lignocellulose regenerated from ILs. Cellulase secreted by the strain showed high resistance to ILs based on phosphate and sulfate radicals, evidencing of a high conformational stability in relevant media. Gratifyingly, F. oxysporum BN can directly convert IL-pretreated rice straw to bioethanol via consolidated bioprocessing (I-CBP). At optimum fermentation condition, a maximum ethanol yield of 0.125 g ethanol g(-1) of rice straw was finally obtained, corresponding to 64.2% of the theoretical yield.
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Jiaxing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China; Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University, Huaian 223300, China.
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Hu
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xia
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, 111 Changjiangxi Road, Huaian 223300, China.
authors.forename: Benlin
authors.initials: B
authors.lastname: Dai
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, 30 Puzhunan Road, Nanjing 210000, China. Electronic address: wubin1977@njtech.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002480:Cellulase / Q000378:metabolism*; D004795:Enzyme Stability / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism; D005670:Fusarium / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D007093:Imidazoles / Q000633:toxicity; D052578:Ionic Liquids / Q000633:toxicity*; D008031:Lignin / Q000378:metabolism*; D012275:Oryza / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D014866:Waste Products
chemical_list: D007093:Imidazoles; D052578:Ionic Liquids; D014866:Waste Products; C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D002480:Cellulase; C518739:1-ethyl-3-methylimidazolium
keywords: Bioethanol; Cellulase; Fusarium oxysporum; Ionic liquid tolerance; Lignocellulose