title: Dhasingreoside: new flavonoid from the stems and leaves of Gaultheria fragrantissima.
abstract: A new flavonoid, dhasingreoside (1) and seven known compounds, quercetin 3-O-β-D-galacturonopyranoside (2), quercetin 3-O-β-D-galactopyranoside (3), quercetin 3-O-β-D-glucuronopyranoside (4), quercetin 3-O-α-L-rhamnopyranoside (5), (-)-epicatechin (6), salicylic acid (7) and gaultherin (8), have been isolated from the shade-dried stems and leaves of Gaultheria fragrantissima, commonly known as 'Dhasingre' in Nepal. The structures were elucidated on the basis of physical, chemical and spectroscopic methods. Among known compounds, five compounds (3-6 and 8) were isolated for the first time from G. fragrantissima. In vitro antioxidant activity of all the isolated compounds was evaluated by 1,1-diphenyl-2-picrylhydrazyl free radical-scavenging assay. Dhasingreoside (1) and other compounds (2-6) showed significant free radical-scavenging activity.
authors.affiliation: a Graduate School of Pharmaceutical Sciences, Kumamoto University , 5-1 Oe-honmachi, Chuo ku, Kumamoto 862-0973 , Japan.
authors.forename: Fei
authors.initials: F
authors.lastname: Cong
authors.forename: Khem Raj
authors.initials: KR
authors.lastname: Joshi
authors.forename: Hari Prasad
authors.initials: HP
authors.lastname: Devkota
authors.forename: Takashi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Shoji
authors.initials: S
authors.lastname: Yahara
mesh_terms: D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification; D031282:Gaultheria / Q000737:chemistry*; D009390:Nepal; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
chemical_list: D005419:Flavonoids; D016166:Free Radical Scavengers
keywords: DPPH; Dhasingre; Gaultheria fragrantissima; dhasingreoside; in vitro