title: In search of pathogens: transcriptome-based identification of viral sequences from the pine processionary moth (Thaumetopoea pityocampa).
abstract: Thaumetopoea pityocampa (pine processionary moth) is one of the most important pine pests in the forests of Mediterranean countries, Central Europe, the Middle East and North Africa. Apart from causing significant damage to pinewoods, T. pityocampa occurrence is also an issue for public and animal health, as it is responsible for dermatological reactions in humans and animals by contact with its irritating hairs. High throughput sequencing technologies have allowed the fast and cost-effective generation of genetic information of interest to understand different biological aspects of non-model organisms as well as the identification of potential pathogens. Using these technologies, we have obtained and characterized the transcriptome of T. pityocampa larvae collected in 12 different geographical locations in Turkey. cDNA libraries for Illumina sequencing were prepared from four larval tissues, head, gut, fat body and integument. By pooling the sequences from Illumina platform with those previously published using the Roche 454-FLX and Sanger methods we generated the largest reference transcriptome of T. pityocampa. In addition, this study has also allowed identification of possible viral pathogens with potential application in future biocontrol strategies.
authors.affiliation: Department of Genetics, Universitat de València, Dr Moliner 50, 46100 Burjassot, Spain. agatajak@gmail.com.
authors.forename: Agata K
authors.initials: AK
authors.lastname: Jakubowska
authors.affiliation: Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey. zihni.demirbag@ktu.edu.tr.
authors.forename: Remziye
authors.initials: R
authors.lastname: Nalcacioglu
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" (IHSM-UMA-CSIC), Consejo Superior de Investigaciones Científicas, Estación Experimental "La Mayora", Algarrobo-Costa, 29750 Málaga, Spain. anabel@eelm.csic.es.
authors.forename: Anabel
authors.initials: A
authors.lastname: Millán-Leiva
authors.affiliation: Department of Genetics, Universitat de València, Dr Moliner 50, 46100 Burjassot, Spain. alexsanzc@gmail.com.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Sanz-Carbonell
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey. muratoglu@ktu.edu.tr.
authors.forename: Hacer
authors.initials: H
authors.lastname: Muratoglu
authors.affiliation: Department of Genetics, Universitat de València, Dr Moliner 50, 46100 Burjassot, Spain. sherrero@uv.es.
authors.forename: Salvador
authors.initials: S
authors.lastname: Herrero
authors.affiliation: Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkey. zihni.demirbag@ktu.edu.tr.
authors.forename: Zihni
authors.initials: Z
authors.lastname: Demirbag
mesh_terms: D000818:Animals; D019295:Computational Biology; D005814:Genes, Viral; D059014:High-Throughput Nucleotide Sequencing; D007814:Larva; D058977:Molecular Sequence Annotation; D009036:Moths / Q000821:virology; D010802:Phylogeny; D059467:Transcriptome; D014421:Turkey; D014780:Viruses / Q000145:classification / Q000235:genetics