title: Enhancement of thermal reversibility and stability of human carbonic anhydrase II by mesoporous nanoparticles.
abstract: Aminopropyl functionalized PEGylated mesoporous silica nanoparticles [H2N-Pr@PEGylated SBA-15] were synthesized and evaluated as a promising biocompatible additive to study the activity and thermal reversibility and stability of human carbonic anhydrase II (HCA II). For this purpose, the additive was prepared by covalent amino propyl functionalization of mesoporous silica nanoparticles (MSNs) bearing PEG moiety as linker. The MSNs was fully characterized using different techniques including transmission electron microscopy, N2 adsorption-desorption measurements, thermal gravimetric analysis, Fourier transform infrared spectroscopy and dynamic light scattering. The average particle size of [H2N-Pr@PEGylated SBA-15] was about 80 nm and showed high loading capacity for HCA II at pH 7.75 as a target protein. The efficiency of [H2N-Pr@PEGylated SBA-15] in improving reversibility of HCA II was investigated by various techniques including UV-vis, 1,8-Anilinonaphtalene Sulfonate (ANS) fluorescence, circular dichroism (CD), and differential scanning calorimetry. Our results showed that [H2N-Pr@PEGylated SBA-15] can increase the protein thermal reversibility and stability. Herein, kinetic studies were applied to confirm the ability of [H2N-Pr@PEGylated SBA-15] in increasing the activity of HCA II at high temperatures. Together our results present the [H2N-Pr@PEGylated SBA-15] as a water-dispersible and efficient additive for improving the activity, and thermal reversibility and stability of enzyme.
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khatibi
authors.affiliation: Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14176, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Ma'mani
authors.affiliation: Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Khodarahmi
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran 14176, Iran; Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Maghami
authors.affiliation: Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi 110025, India.
authors.forename: Faizan
authors.initials: F
authors.lastname: Ahmad
authors.affiliation: Departments of Ophthalmology and Visual Sciences, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
authors.forename: Nader
authors.initials: N
authors.lastname: Sheibani
authors.affiliation: Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran; Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran. Electronic address: moosavi@ut.ac.ir.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Moosavi-Movahedi
mesh_terms: D002152:Calorimetry, Differential Scanning; D024402:Carbonic Anhydrase II / Q000378:metabolism*; D002942:Circular Dichroism; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D016062:Porosity; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D004800:Enzymes, Immobilized; D024402:Carbonic Anhydrase II
keywords: Aminopropyl functionalized PEGylated mesoporous silica nanoparticles; Human carbonic anhydrase; Thermal reversibility and stability