title: Regulated protonation of polyaspartamide derivatives bearing repeated aminoethylene side chains for efficient intracellular siRNA delivery with minimal cytotoxicity.
abstract: The effects of the repeated number (RN) of aminoethylene (AE) units in polyaspartamide side chains were investigated for polyion complex (PIC)-based siRNA delivery. Reduction of the apparent RN from 3 to 2 by thiourea introduction increased a protonatable amine fraction in AE units at endosomal pH, leading to the efficient endosomal escape of siRNA-loaded PICs.
authors.affiliation: School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin, 300384, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Gao
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Takemoto
authors.forename: Qixian
authors.initials: Q
authors.lastname: Chen
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Naito
authors.forename: Hirokuni
authors.initials: H
authors.lastname: Uchida
authors.forename: Xueying
authors.initials: X
authors.lastname: Liu
authors.forename: Kanjiro
authors.initials: K
authors.lastname: Miyata
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kataoka
mesh_terms: D001224:Aspartic Acid / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D006801:Humans; D008156:Luciferases / Q000235:genetics; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000737:chemistry*; D013890:Thiourea / Q000737:chemistry*
chemical_list: D010455:Peptides; D034741:RNA, Small Interfering; D001224:Aspartic Acid; D008156:Luciferases; D013890:Thiourea