title: Two-layer Lab-on-a-chip (LOC) with passive capillary valves for mHealth medical diagnostics.
abstract: There is a new potential to address needs for medical diagnostics in Point-of-Care (PoC) applications using mHealth (Mobile computing, medical sensors, and communications technologies for health care), a mHealth based lab test will require a LOC to perform clinical analysis. In this work, we describe the design of a simple Lab-on-a-chip (LOC) platform for mHealth medical diagnostics. The LOC utilizes a passive capillary valve with no moving parts for fluid control using channels with very low aspect ratios cross sections (i.e., channel width ≫ height) achieved through transitions in the channel geometry via that arrest capillary flow. Using a CO2 laser in raster engraving mode, we have designed and fabricated an eight-channel LOC for fluorescence signal detection fabricated by engraving and combining just two polymer layers. Each of the LOC channels is capable of mixing two reagents (e.g., enzyme and substrate) for various assays. For mHealth detection, we used a mobile CCD detector equipped with LED multispectral illumination in the red, green, blue, and white range. This technology enables the development of low-cost LOC platforms for mHealth whose fabrication is compatible with standard industrial plastic fabrication processes to enable mass production of mHealth diagnostic devices, which may broaden the use of LOCs in PoC applications, especially in global health settings.
authors.affiliation: Division of Biology, Office of Science and Engineering, FDA, 10903 New Hampshire Avenue, Silver Spring, MD, 20993, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Balsam
authors.forename: Hugh Alan
authors.initials: HA
authors.lastname: Bruck
authors.forename: Avraham
authors.initials: A
authors.lastname: Rasooly
mesh_terms: D040421:Cell Phone; D003906:Developing Countries; D004798:Enzymes / Q000032:analysis; D004867:Equipment Design; D019793:Fluorescein / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D014943:Global Health; D006801:Humans; D056656:Lab-On-A-Chip Devices / Q000191:economics*; D054020:Lasers, Gas; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055616:Microtechnology; D055096:Optical Devices / Q000191:economics*; D019095:Point-of-Care Systems; D017216:Telemedicine
chemical_list: D004798:Enzymes; D005456:Fluorescent Dyes; D019793:Fluorescein