title: [Effects on expression of osteogenesisgene in the osteoblast with endoplasmic reticulum stress induced by fluoride].
abstract: OBJECTIVE
  To explore the gene expressions of endoplasmic reticulum stress and differentiation in osteoblast treated by excess fluoride.
  
  
  METHODS
  Using primary cultured human osteoblasts for fluorosis model in vitro, apoptosis was inspected by flow cytometer, and RNA was extracted for examination of the unfolded protein response and bone differentiation genes.
  
  
  RESULTS
  Fluoride could cause endoplasm reticulum stress in osteoblasts by 15 genes upregulated, 1 gene downregulated. These genes involved PERK, IRE1 and ATF6 signaling pathways of endoplasmic reticulum stress. Meanwhile 32 osteogenesis genes were upregulated, and 2 genes downregulated, involving collagen, matrix metalloproteinase, integrin, bone morphogenetic protein, vascular endothelial growth factor, and tumor necrosis factor gene.
  
  
  CONCLUSIONS
  Excess fluoride can cause endoplasmic stress in osteoblast, while have an impact on the gene expression of osteogenesis.
authors.affiliation: Department of histology and embryology of Preclinical Institute, Xinjiang Medical University, Urumqi 830002, China. 49669129@qq.com
authors.forename: Yalou
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaona
authors.initials: X
authors.lastname: Sun
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Shumei
authors.initials: S
authors.lastname: Feng
authors.forename: Libin
authors.initials: L
authors.lastname: Liao
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Feng
authors.initials: F
authors.lastname: Deng
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Qin
authors.forename: Jinjie
authors.initials: J
authors.lastname: Zhong
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D005458:Fluoride Poisoning; D005459:Fluorides / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000502:physiology*; D010710:Phosphates; D015398:Signal Transduction; D056811:Unfolded Protein Response / Q000187:drug effects* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A
chemical_list: D010710:Phosphates; D042461:Vascular Endothelial Growth Factor A; C012980:fluorophosphate; D005459:Fluorides