title: Type I collagen gel protects murine fibrosarcoma L929 cells from TNFα-induced cell death.
abstract: Murine fibrosarcoma L929 cells have been used to test efficacy of proinflammatory cytokine TNFα. In the present study, we reported on protective effect of type I collagen gel used as L929 cell culture. L929 cell grew and proliferated well on collagen gel. However, the L929 cells exhibited cobblestone-like morphology which was much different from the spread fusiform shape when cultured on conventional cell dishes as well as the cells tended to aggregate. On conventional cell culture dishes, the cells treated with TNFα became round in shape and eventually died in a necroptotic manner. The cells cultured on collagen gel, however, were completely unaffected. TNFα treatment was reported to induce autophagy in L929 cells on the plastic dish, and therefore we investigated the effect of collagen gel on induction of autophagy. The results indicated that autophagy induced by TNFα treatment was much reduced when the cells were cultured on collagen gel. In conclusion, type I collagen gel protected L929 cell from TNFα-induced cell death.
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Hong-Ju
authors.initials: HJ
authors.lastname: Wang
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Wen-Qi
authors.initials: WQ
authors.lastname: He
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Chen
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Liu
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Xia
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Nippi Research Institute of Biomatrix, Toride, Ibaraki 302-0017, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Fujisaki
authors.affiliation: Nippi Research Institute of Biomatrix, Toride, Ibaraki 302-0017, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.affiliation: Institute for Clinical and Biomedical Sciences, Kyoto 603-8072, Japan.
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Tashiro
authors.affiliation: Department of Clinical and Pharmaceutical Sciences, Showa Pharmaceutical University, Tokyo 194-8543, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Onodera
authors.affiliation: China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: ikejimat@vip.sina.com.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ikejima
mesh_terms: D000818:Animals; D001343:Autophagy / Q000187:drug effects; D018929:Cell Culture Techniques; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D024042:Collagen Type I / Q000494:pharmacology*; D005354:Fibrosarcoma / Q000188:drug therapy / Q000276:immunology; D005782:Gels / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D020011:Protective Agents / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology*
chemical_list: D024042:Collagen Type I; D005782:Gels; D020011:Protective Agents; D014409:Tumor Necrosis Factor-alpha
keywords: Autophagy; Collagen gel; L929; Necroptosis; TNFα