title: Oleuropein suppresses LPS-induced inflammatory responses in RAW 264.7 cell and zebrafish.
abstract: Oleuropein is one of the primary phenolic compounds present in olive leaf. In this study, the anti-inflammatory effect of oleuropein was investigated using lipopolysaccharide (LPS)-stimulated RAW 264.7 and a zebrafish model. The inhibitory effect of oleuropein on LPS-induced NO production in macrophages was supported by the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). In addition, our enzyme immunoassay showed that oleuropein suppressed the release of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Oleuropein inhibited the translocation of p65 by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Oleuropein also decreased activation of ERK1/2 and JNK, which are associated with LPS-induced inflammation, and its downstream gene of AP-1. Furthermore, oleuropein inhibited LPS-stimulated NO generation in a zebrafish model. Taken together, our results demonstrated that oleuropein could reduce inflammatory responses by inhibiting TLR and MAPK signaling, and may be used as an anti-inflammatory agent.
authors.affiliation: Department of Biomedical Science, CHA University , Kyonggi 463-836, South Korea.
authors.forename: Su-Jung
authors.initials: SJ
authors.lastname: Ryu
authors.forename: Hyeon-Son
authors.initials: HS
authors.lastname: Choi
authors.forename: Kye-Yoon
authors.initials: KY
authors.lastname: Yoon
authors.forename: Ok-Hwan
authors.initials: OH
authors.lastname: Lee
authors.forename: Kui-Jin
authors.initials: KJ
authors.lastname: Kim
authors.forename: Boo-Yong
authors.initials: BY
authors.lastname: Lee
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000276:immunology; D007249:Inflammation / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D057888:Iridoid Glucosides; D039823:Iridoids / Q000008:administration & dosage*; D008070:Lipopolysaccharides / Q000009:adverse effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D031658:Olea / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry; D015027:Zebrafish
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D057888:Iridoid Glucosides; D039823:Iridoids; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; C002769:oleuropein; D051546:Cyclooxygenase 2
keywords: NF-κB(p-65); RAW 264.7 cell; anti-inflammation; oleuropein; zebrafish