title: Gene sequence analysis and screening of feature genes in spinal cord injury.
abstract: The aim of the present study was to screen for feature genes associated with spinal cord injury (SCI), in order to identify the underlying pathogenic mechanisms. Differentially expressed genes were screened for using pre‑processing data. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis was performed to analyze and identify the genes involved in pathways associated with SCI. Subsequently, Gene Ontology enrichment analysis and Uniprot tissue analysis were used to screen out genes specifically expressed in spinal cord tissue. In addition, a protein‑protein interaction network was used to demonstrate possible associations among SCI‑associated feature genes. Finally, a link was identified between feature genes and SCI by analyzing protein domains in coding areas of the three feature genes. The cytochrome c oxidase subunit Va, adenosine triphosphate (ATP) synthase, H+ transporting, mitochondrial F1 complex, α subunit 1 and cardiac muscle and mitochondrial β‑F1‑ATPase may be downregulated in SCI, resulting in destruction of the mitochondrial electron transport chain and membrane‑bound enzyme complexes/ion transporters, thus, affecting the normal function of nerves. The three screened feature genes have the potential to become candidate target molecules to monitor, diagnose and treat SCI and may be beneficial for the early diagnosis and therapeutic control of the condition.
authors.affiliation: Department of Spine Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Zongde
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Anesthesia, Fuzhou General Hospital of Nanjing Military Command Affiliated to Fujian Medical University, Fuzhou, Fujian 350025, P.R. China.
authors.forename: Ren
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Spine Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Spine Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Yinchuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Spine Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Spine Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D004195:Disease Models, Animal; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D051381:Rats; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome