title: The activity of paraoxonase type 1 (PON-1) in boar seminal plasma and its relationship with sperm quality, functionality, and in vivo fertility.
abstract: Paraoxonase 1 (PON-1) is a hydrolytic enzyme present in body fluids, capable of protecting cells against oxidative stress. The hypothesis was hereby to test that PON-1, present in seminal plasma (SP), acts protecting boar spermatozoa when showing a reasonable high activity in the ejaculate. SP-PON-1 activity differed (p < 0.001) among boars (from 0.10 to 0.29 IU/mL). Intra-boar variability was also observed (p < 0.05), but only in two of the 15 boars. SP-PON-1 activity differed among ejaculate portions, showing the spermatozoa-peak portion of spermatozoa-rich ejaculate fraction the highest levels (0.35 ± 0.03 IU/mL, ranging from 0.12 to 0.69) and the post-sperm ejaculate fraction the lowest levels (0.12 ± 0.01 IU/mL, ranging from 0.03 to 0.21). SP-PON-1 activity was positively correlated with the percentage of spermatozoa with rapid and progressive movement (p < 0.01) and negatively correlated with the generation of intracellular reactive oxygen species (p < 0.01) in semen samples after 72 h of liquid storage. SP-PON-1 activity was highest (p < 0.01) in boars with highest farrowing rates. In conclusion, SP-PON-1 activity differed among boars and ejaculate fractions/portions. SP-PON-1 activity was positively correlated with sperm quality and functionality of liquid-stored semen samples and it evidenced a positive association with in vivo fertility.
authors.affiliation: Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Murcia, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Barranco
authors.forename: A
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.forename: C
authors.initials: C
authors.lastname: Perez-Patiño
authors.forename: D V
authors.initials: DV
authors.lastname: Alkmin
authors.forename: J J
authors.initials: JJ
authors.lastname: Ceron
authors.forename: E A
authors.initials: EA
authors.lastname: Martinez
authors.forename: H
authors.initials: H
authors.lastname: Rodriguez-Martinez
authors.forename: J
authors.initials: J
authors.lastname: Roca
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D005298:Fertility; D008297:Male; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa; D013552:Swine
chemical_list: D043303:Aryldialkylphosphatase
keywords: boar; paraoxonase type 1; semen storage; seminal plasma; sperm functionality; sperm quality