title: Impairment of preimplantation porcine embryo development by histone demethylase KDM5B knockdown through disturbance of bivalent H3K4me3-H3K27me3 modifications.
abstract: KDM5B (JARID1B/PLU1) is a H3K4me2/3 histone demethylase that is implicated in cancer development and proliferation and is also indispensable for embryonic stem cell self-renewal, cell fate, and murine embryonic development. However, little is known about the role of KDM5B during preimplantation embryo development. Here we show that KDM5B is critical to porcine preimplantation development. KDM5B was found to be expressed in a stage-specific manner, consistent with demethylation of H3K4me3, with the highest expression being observed from the 4-cell to the blastocyst stages. Knockdown of KDM5B by morpholino antisense oligonucleotides injection impaired porcine embryo development to the blastocyst stage. The impairment of embryo development might be caused by increased expression of H3K4me3 at the 4-cell and blastocyst stages, which disturbs the balance of bivalent H3K4me3-H3K27me3 modifications at the blastocyst stage. Decreased abundance of H3K27me3 at blastocyst stage activates multiple members of homeobox genes (HOX), which need to be silenced for faithful embryo development. Additionally, the histone demethylase KDM6A was found to be upregulated by knockdown of KDM5B, which indicated it was responsible for the decreased abundance of H3K27me3 at the blastocyst stage. The transcriptional levels of Ten-Eleven Translocation gene family members (TET1, TET2, and TET3) are found to be increased by knockdown of KDM5B, which indicates cross talk between histone modifications and DNA methylation. The studies above indicate that KDM5B is required for porcine embryo development through regulating the balance of bivalent H3K4me3-H3K27me3 modifications.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Hongyong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xianlong
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Swine Resource and Research Center & Division of Animal Science, University of Missouri, Columbia, Missouri Department of Biology, Northeastern University, Boston, Massachusetts.
authors.forename: Kyle B
authors.initials: KB
authors.lastname: Dobbs
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Guosong
authors.initials: G
authors.lastname: Qin
authors.affiliation: National Swine Resource and Research Center & Division of Animal Science, University of Missouri, Columbia, Missouri.
authors.forename: Kristin
authors.initials: K
authors.lastname: Whitworth
authors.affiliation: National Swine Resource and Research Center & Division of Animal Science, University of Missouri, Columbia, Missouri.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Walters
authors.affiliation: National Swine Resource and Research Center & Division of Animal Science, University of Missouri, Columbia, Missouri Pratherr@missouri.edu.
authors.forename: Randall S
authors.initials: RS
authors.lastname: Prather
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China Zhaojg@ioz.ac.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics / Q000502:physiology*; D005260:Female; D017353:Gene Deletion; D055785:Gene Knockdown Techniques; D005801:Genes, Homeobox / Q000235:genetics / Q000502:physiology; D056466:Histone Demethylases / Q000235:genetics / Q000502:physiology*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000235:genetics / Q000502:physiology*; D008745:Methylation; D008969:Molecular Sequence Data; D013552:Swine / Q000196:embryology* / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D056466:Histone Demethylases; D056484:Jumonji Domain-Containing Histone Demethylases
keywords: H3K4me3; KDM5B; embryonic development; histone demethylase; pig