title: Label-free detection microarray for novel peptide ligands screening base on MS-SPRi combination.
abstract: Peptides ligands with high affinity and high specificity towards specific targets is catching a good deal of interests in biomedical field. Traditional peptide screening procedure involves selection, sequencing and characterization and each step is time-consuming and labor-intensive. The combination between different analytical methods could provide an integrated plan for efficient peptide screening. We report herein a label-free detection microarray system to facilitate the whole one-bead-one-compound (OBOC) peptide screening process. A microwell array chip with two identical units can trap the candidate peptide beads in one-well-one-bead manner. Peptides on beads were photo-released in situ in the well and partly transferred to two identical chips for Surface Plasmon Resonance imaging (SPRi), and peptide left in the bi-unit microwell array chip was remain for in situ single bead sequencing by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Using the bi-unit imprinted chip system, affinity peptides towards AD protein were efficiently screened out both qualitatively and quantitatively from 10(4) candidates. The method provides a universal solution for high efficiency and high throughput ligands screening.
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China. Electronic address: wangwz@nanoctr.cn.
authors.forename: Weizhi
authors.initials: W
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Zewen
authors.initials: Z
authors.lastname: Wei
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Xiangli
authors.initials: X
authors.lastname: Bu
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China.
authors.forename: Qiaojun
authors.initials: Q
authors.lastname: Fang
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience and Technology of China, Beijing 100190, China; Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China; Institute for Systems Biology, 401 Terry Avenue N, Seattle, WA 98109, USA. Electronic address: huzy@nanoct.cn.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Hu
mesh_terms: D008024:Ligands; D009842:Oligopeptides / Q000737:chemistry*; D040081:Protein Array Analysis / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D020349:Surface Plasmon Resonance
chemical_list: D008024:Ligands; D009842:Oligopeptides
keywords: Bi-unit; Label-free detection; MALDI-TOF-MS; Microarray; SPRi