title: Functionalized gold nanoparticle-polypyrrole nanobiocomposite with high effective surface area for electrochemical/pH dual stimuli-responsive smart release of insulin.
abstract: A novel functionalized gold nanoparticle-polypyrrole-nanobiocomposite (PPy-FGNP-NBC) with large effective surface area was fabricated for electrical/pH dual stimuli-responsive local delivery of insulin. The fabrication method involves simple electrodeposition and immobilization processes without use of organic solvents. Release studies based on the nature of insulin-surface binding indicated that release was promoted for PPy-FGNP-NBC. Kinetics analysis showed that release of insulin strongly affected by applying external potential stimuli. Also, the insulin release was under influence of pH and was slowed down under lower pH. This pH-sensitivity was remarkably increased by applying potential. Based on in vitro release study under applied potential condition, insulin release in the artificial gastric juice is significantly slower than that in the artificial intestinal fluid. Circular dichroism analysis showed that insulin retained its original conformation during electrochemically stimulated release.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Shamaeli
authors.affiliation: Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran. Electronic address: alizaden@modares.ac.ir.
authors.forename: Naader
authors.initials: N
authors.lastname: Alizadeh
mesh_terms: D002942:Circular Dichroism; D065546:Drug Liberation; D055664:Electrochemical Techniques; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000008:administration & dosage / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D007328:Insulin; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D006046:Gold
keywords: Dual stimuli-responsive; Functionalized gold nanoparticle; Insulin; Polypyrrole; Release