title: A method for high-throughput analysis of chronological aging in Schizosaccharomyces pombe.
abstract: The measurement of chronological life span (CLS) in Schizosaccharomyces pombe is traditionally performed by plating back aliquots of aging liquid cultures on solid medium and counting the number of colony-forming units (CFU). However, this method is labor and cost intensive and therefore not amenable to high-throughput screening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging.
authors.affiliation: Institut für Biologie, Humboldt-Universität Berlin, Chausseestr. 117, 10115, Berlin, Germany.
authors.forename: Jessica
authors.initials: J
authors.lastname: Stephan
authors.forename: Ann E
authors.initials: AE
authors.lastname: Ehrenhofer-Murray
mesh_terms: D057166:High-Throughput Screening Assays; D008136:Longevity / Q000235:genetics*; D012568:Schizosaccharomyces / Q000502:physiology*