title: Novel genotype-phenotype associations in human cancers enabled by advanced molecular platforms and computational analysis of whole slide images.
abstract: Technological advances in computing, imaging, and genomics have created new opportunities for exploring relationships between histology, molecular events, and clinical outcomes using quantitative methods. Slide scanning devices are now capable of rapidly producing massive digital image archives that capture histological details in high resolution. Commensurate advances in computing and image analysis algorithms enable mining of archives to extract descriptions of histology, ranging from basic human annotations to automatic and precisely quantitative morphometric characterization of hundreds of millions of cells. These imaging capabilities represent a new dimension in tissue-based studies, and when combined with genomic and clinical endpoints, can be used to explore biologic characteristics of the tumor microenvironment and to discover new morphologic biomarkers of genetic alterations and patient outcomes. In this paper, we review developments in quantitative imaging technology and illustrate how image features can be integrated with clinical and genomic data to investigate fundamental problems in cancer. Using motivating examples from the study of glioblastomas (GBMs), we demonstrate how public data from The Cancer Genome Atlas (TCGA) can serve as an open platform to conduct in silico tissue-based studies that integrate existing data resources. We show how these approaches can be used to explore the relation of the tumor microenvironment to genomic alterations and gene expression patterns and to define nuclear morphometric features that are predictive of genetic alterations and clinical outcomes. Challenges, limitations, and emerging opportunities in the area of quantitative imaging and integrative analyses are also discussed.
authors.affiliation: 1] Department of Biomedical Informatics, Emory University, Atlanta, GA, USA [2] Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA [3] Winship Cancer Institute, Emory University, Atlanta, GA, USA.
authors.forename: Lee A D
authors.initials: LA
authors.lastname: Cooper
authors.affiliation: Department of Biomedical Informatics, Emory University, Atlanta, GA, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Kong
authors.affiliation: 1] Department of Biomedical Informatics, Emory University, Atlanta, GA, USA [2] Winship Cancer Institute, Emory University, Atlanta, GA, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Gutman
authors.affiliation: Department of Neurology, Emory University, Atlanta, GA, USA.
authors.forename: William D
authors.initials: WD
authors.lastname: Dunn
authors.affiliation: Department of Biomedical Informatics, Emory University, Atlanta, GA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Nalisnik
authors.affiliation: 1] Department of Biomedical Informatics, Emory University, Atlanta, GA, USA [2] Winship Cancer Institute, Emory University, Atlanta, GA, USA [3] Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Brat
mesh_terms: D005821:Genetic Techniques; D023281:Genomics; D006651:Histocytochemistry; D006801:Humans; D009369:Neoplasms / Q000737:chemistry / Q000175:diagnosis / Q000235:genetics / Q000473:pathology