title: Identifying reactive peptides from phage-displayed libraries.
abstract: Phage display enables the synthesis, selection, and screening of large, polypeptide libraries (>1 × 10(10) different members). Selections from such libraries can identify binding partners to essentially any desired target (Sarikaya et al., Annu Rev Mater Res 34:373-408, 2004; Deutscher, Chem Rev 110:3196-3211, 2010). Peptides with affinity or reactivity to small molecule probes are attractive for numerous uses including the targeted, site-specific labeling of proteins. Here, we describe selection and screening protocols for the identification of short peptides that can selectively bind to and/or react with small molecules.
authors.affiliation: Science and Math Division, West Valley College, Saratoga, CA, USA.
authors.forename: Glenn M
authors.initials: GM
authors.lastname: Eldridge
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Weiss
mesh_terms: D000818:Animals; D019020:Directed Molecular Evolution / Q000379:methods*; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics*; D019151:Peptide Library
chemical_list: D009842:Oligopeptides; D019151:Peptide Library