title: Overexpression, purification, crystallization and preliminary X-ray crystallographic analysis of SF173 from Shigella flexneri.
abstract: Shigella flexneri is a Gram-negative, anaerobic bacterium in the genus Shigella that can cause diarrhoea in humans. SF173, a hypothetical protein from S. flexneri 5a strain M90T, has been cloned, overexpressed, purified and crystallized as a part of laboratory-scale structural genomics project. The SF173 protein was crystallized using the sitting-drop vapour-diffusion method in the presence of 0.8 M succinic acid pH 7.0 at 293 K. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 1.47 Å resolution and belonged to space group I432, with unit-cell parameters a=b=c=110.245 Å.
authors.affiliation: Department of Molecular Science andTechnology, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Ha-Neul
authors.initials: HN
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Jeong-Gi
authors.initials: JG
authors.lastname: An
authors.affiliation: Department of Molecular Science andTechnology, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Yoo-Sup
authors.initials: YS
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Seung-Hyeon
authors.initials: SH
authors.lastname: Seok
authors.affiliation: College of Pharmacy, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Hee-Seop
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Department of Molecular Science andTechnology, Ajou University, Suwon, Kyeonggi 443-749, Republic of Korea.
authors.forename: Min-Duk
authors.initials: MD
authors.lastname: Seo
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D014176:Protein Biosynthesis; D012763:Shigella flexneri
chemical_list: D001426:Bacterial Proteins
keywords: SF173; Shigella flexneri