title: Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay.
abstract: This manuscript describes synthesis and followed application of silica-coated liposomes loaded with quantum dots as a perspective label for immunoaasay. The hollow spherical structure of liposomes makes them an attractive package material for encapsulation of multiple water-insoluble quantum dots and amplifying the analytical signal. Silica coverage ensures the stability of the loaded liposomes against fusion and internal leakage during storage, transporting, application and also provides groups for bioconugation. For the first time these nanostructures were employed for the sensitive multiplex immunochemical determination of two analytes. As a model system mycotoxins zearalenone and aflatoxin B1 were detected in cereals. For simplification of multiassay results' evaluation the silanized liposomed loaded with QDs of different colors were used. The IC50 values for the simultaneous determination of zearalenone and aflatoxin B1 were 16.2 and 18 µg kg(-1) for zearalenone and 2.2 and 2.6 µg kg(-1) for aflatoxin B1 in wheat and maize, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.
authors.affiliation: Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium. Electronic address: beloglazovanv@gmail.com.
authors.forename: N V
authors.initials: NV
authors.lastname: Beloglazova
authors.affiliation: Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Goryacheva
authors.affiliation: Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: E S
authors.initials: ES
authors.lastname: Speranskaya
authors.affiliation: Ghent University, Department of Inorganic and Physical Chemistry, Krijgslaan 281 S3, 9000 Ghent, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Aubert
authors.affiliation: OJSC CSRIT Technomash, "Neuronet", 121108 Moscow, Russia.
authors.forename: P S
authors.initials: PS
authors.lastname: Shmelin
authors.affiliation: OJSC CSRIT Technomash, "Neuronet", 121108 Moscow, Russia.
authors.forename: V R
authors.initials: VR
authors.lastname: Kurbangaleev
authors.affiliation: Saratov State University, Chemistry Faculty, Department of General and Inorganic Chemistry, Astrakhanskaya 83, 410012 Saratov, Russia.
authors.forename: I Yu
authors.initials: IY
authors.lastname: Goryacheva
authors.affiliation: Ghent University, Faculty of Pharmaceutical Sciences, Laboratory of Food Analysis, Ottergemsesteenweg 460, 9000 Ghent, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: De Saeger
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D002853:Chromatography, Liquid; D055767:Immobilized Proteins / Q000276:immunology; D007118:Immunoassay; D007074:Immunoglobulin G / Q000276:immunology; D008081:Liposomes / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D015025:Zearalenone / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
chemical_list: D055767:Immobilized Proteins; D007074:Immunoglobulin G; D008081:Liposomes; D015025:Zearalenone; D012822:Silicon Dioxide; D016604:Aflatoxin B1
keywords: Cereals; Liposomes; Multiplex immunoasay; Mycotoxin; Quantum dots; Silica-coated liposomes