title: Mass spectrometry detection of minor new meridianins from the Antarctic colonial ascidians Aplidium falklandicum and Aplidium meridianum.
abstract: Taking into account the broad biological activities found in the meridianin indole alkaloids isolated to date, we have re-examined the organic extracts of an Antarctic collection of the tunicates Aplidium meridianum and A. falklandicum (Chordata: Ascidiacea) by HPLC in conjunction with a high-resolution mass spectrometer (HPLC-MS). A new set of analogs of meridianins A-G has been detected, and their structures are proposed on the basis of the molecular formulae identified by LC-HRMS analysis using a C18 column with a gradient of water/acetonitrile and an LTQ-FT-MS Orbitrap detector. Remarkably, dimers derived from meridianin A and from meridianin B or E were also detected. Our findings provide further evidence of the broad variability within the meridianin-like derivatives of this highly bioactive alkaloid family.
authors.affiliation: Department of Animal Biology (Invertebrates) & Biodiversity Research Institute (IrBio), Faculty of Biology, University of Barcelona, Av. Diagonal 643, ES-08028, Barcelona, Catalonia, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Núñez-Pons
authors.forename: Rosa María
authors.initials: RM
authors.lastname: Nieto
authors.forename: Conxita
authors.initials: C
authors.lastname: Avila
authors.forename: Carlos
authors.initials: C
authors.lastname: Jiménez
authors.forename: Jaime
authors.initials: J
authors.lastname: Rodríguez
mesh_terms: D000818:Animals; D000864:Antarctic Regions; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019281:Dimerization; D026121:Indole Alkaloids / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D015394:Molecular Structure; D011743:Pyrimidines / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014561:Urochordata / Q000737:chemistry*
chemical_list: D026121:Indole Alkaloids; D011743:Pyrimidines; C560347:meridianin A; C522699:meridianin G
keywords: A. falklandicum; Aplidium meridianum; FTMS-Orbitrap; LC-MS; indole alkaloids; marine natural products; meridianin-dimers; meridianins