title: Cell penetrating peptide conjugated liposomes as transdermal delivery system of Polygonum aviculare L. extract.
abstract: In this study, Polygonum aviculare L. extract, which has superior antioxidative and cellular membrane protective activity, was loaded onto cell penetrating peptide (CPP) conjugated liposomes to enhance transdermal delivery. The physical characteristics of typical liposomes and CPP-conjugated liposomes containing P. aviculare extract were evaluated. The particle sizes of both liposomes were approximately 150 nm. Whereas the zeta potential of typical liposomes was -45 mV, that of CPP-conjugated liposomes was +42 mV. The loading efficiency of P. aviculare extract in both liposomes was calculated to be about 83%. Fluorescent-labeled liposomes were prepared to evaluate cellular uptake and skin permeation efficiency. Using flow cytometry, we found that CPP-conjugated liposomes improved cellular uptake of the fluorescent dye as compared with the typical liposomes. In addition, the skin permeation of CPP-conjugated liposomes was proved higher than that of typical liposomes by confocal laser scanning microscopy studies and Franz diffusion cell experiments. The improved cellular uptake and skin permeation of the CPP-conjugated liposomes were due to the cationic arginine-rich peptide. In vivo studies also determined that the CPP-conjugated liposomes were more effective in depigmentation and anti-wrinkle studies than typical liposomes. These results indicate that the CPP-conjugated liposomes could be effective for transdermal drug delivery of antioxidant and anti-aging therapeutics.
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Soon Sik
authors.initials: SS
authors.lastname: Kwon
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Kim
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Bong Ju
authors.initials: BJ
authors.lastname: Kong
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Kyeong Jin
authors.initials: KJ
authors.lastname: Kim
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Geun Young
authors.initials: GY
authors.lastname: Noh
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Na Ri
authors.initials: NR
authors.lastname: Im
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Lim
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea.
authors.forename: Ji Hoon
authors.initials: JH
authors.lastname: Ha
authors.affiliation: R&D Unit, AMOREPACIFIC Co., Yongin-Si, Gyeonggi-Do 446-729, South Korea. Electronic address: junohpeterkim@gmail.com.
authors.forename: Junoh
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Fine Chemistry, College of Nature and Life Science & Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science and Technology, 232 Gongreung-ro, Nowon-gu, Seoul 139-743, South Korea. Electronic address: snpark@seoultech.ac.kr.
authors.forename: Soo Nam
authors.initials: SN
authors.lastname: Park
mesh_terms: D000818:Animals; D002470:Cell Survival; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D006801:Humans; D015603:Keratinocytes; D008081:Liposomes / Q000737:chemistry*; D051379:Mice; D008812:Mice, Hairless; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000493:pharmacokinetics*; D028502:Polygonum / Q000737:chemistry*; D012869:Skin Absorption; D015595:Skin Aging / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D057846:Cell-Penetrating Peptides; D008081:Liposomes; D010936:Plant Extracts
keywords: Anti-wrinkle; Cell penetrating peptide; Depigmentation; Liposome; Polygonumaviculare L.; Transdermal delivery