title: First finding of Trichinella pseudospiralis in the Neotropical region.
abstract: Prior to this study, only encapsulated species of Trichinella had been found in South America, i.e., T. spiralis and T. patagoniensis. Here we report the molecular identification of a non-encapsulated isolate of Trichinella from a domestic pig in Argentina. The multiplex PCR technique and the analysis of mitochondrial and nuclear DNA sequences revealed that it belongs to T. pseudospiralis, which parasitises birds and mammals from Australian, Nearctic, and Palaearctic regions. Interestingly, the isolate is closely related to the Palaearctic population. This is the first report of a non-encapsulated species of Trichinella from the Neotropical region.
authors.affiliation: Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563 (1281), Buenos Aires, Argentina. Electronic address: silkri@anlis.gov.ar.
authors.forename: Silvio J
authors.initials: SJ
authors.lastname: Krivokapich
authors.affiliation: Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563 (1281), Buenos Aires, Argentina.
authors.forename: Cinthia L Gonzalez
authors.initials: CL
authors.lastname: Prous
authors.affiliation: Departamento de Parasitología, Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud, "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563 (1281), Buenos Aires, Argentina.
authors.forename: Graciana M
authors.initials: GM
authors.lastname: Gatti
authors.affiliation: Hospital Dr. José Formenti, Julio Roca 1487(9405), El Calafate, Santa Cruz, Argentina.
authors.forename: Luisa
authors.initials: L
authors.lastname: Saldía
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology*; D014234:Trichinella / Q000145:classification*; D014235:Trichinellosis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D016054:DNA, Protozoan
keywords: Argentina; Cytochrome c-oxidase subunit I; D3 domain of nuclear rDNA; Domestic pig; Expansion segment V; Trichinella pseudospiralis