title: Chemical genomic profiling via barcode sequencing to predict compound mode of action.
abstract: Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.
authors.affiliation: Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WEI4152, 1552 University Ave., Madison, WI, 53726, USA, jpiotrowski@wisc.edu.
authors.forename: Jeff S
authors.initials: JS
authors.lastname: Piotrowski
authors.forename: Scott W
authors.initials: SW
authors.lastname: Simpkins
authors.forename: Sheena C
authors.initials: SC
authors.lastname: Li
authors.forename: Raamesh
authors.initials: R
authors.lastname: Deshpande
authors.forename: Sean J
authors.initials: SJ
authors.lastname: McIlwain
authors.forename: Irene M
authors.initials: IM
authors.lastname: Ong
authors.forename: Chad L
authors.initials: CL
authors.lastname: Myers
authors.forename: Charlie
authors.initials: C
authors.lastname: Boone
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Andersen
mesh_terms: D055808:Drug Discovery / Q000379:methods*; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D009154:Mutation / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics*