title: [Inhibitory effect of chloroquine on airway hyperresponsiveness in asthmatic mice].
abstract: OBJECTIVE
  To investigate the effect of chloroquine on airway hyperresponsiveness in asthmatic mice and explore the possible mechanism.
  
  
  METHODS
  Balb/c mouse models of asthma established using OVA received intraperitoneal injections of chloroquine, dexamethasone, or both prior to OVA challenge. Within 24 h after the final challenge, airway hyper- responsiveness (AHR) of the mice was assessed, and the total cell count and the counts of different cell populations in the bronchoalveolar lavage fluid (BALF) were determined under light microscopy. The severity of lung inflammation was evaluated using HE staining, and the concentrations of IL-6 and PGF2α in the BALF were detected by enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  Chloroquine pretreatment significantly decreased AHR (P<0.001) in the asthmatic mice and reduced the total cell count (P<0.01), eosinophils (P<0.001), neutrophils (P<0.01), and PGF2α levels in the BALF. Chloroquine combined with low-dose dexamethasone significantly lessened inflammations around the bronchioles (P<0.05) and blood vessels (P<0.01) in the lung tissue, and obviously lowered IL-6 (P<0.05) and PGF2α (P<0.001) in the BALF in the asthmatic mice.
  
  
  CONCLUSIONS
  Chloroquine can inhibit AHR in asthmatic mice and produce better anti-inflammatory effect when combined with dexamethasone for treatment of neutrophilic asthma.
authors.affiliation: Department of Respiratory Medicine, Children's Hospital Affiliated to Chongqing Medical University, Chongqing 400014, China.E-mail: 1103751123@qq.com.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Sun
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Hu
authors.forename: Li-Jia
authors.initials: LJ
authors.lastname: Wang
authors.forename: En-Mei
authors.initials: EM
authors.lastname: Liu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fu
mesh_terms: D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002738:Chloroquine / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D004804:Eosinophils / Q000166:cytology; D007249:Inflammation / Q000473:pathology; D015850:Interleukin-6 / Q000378:metabolism; D007958:Leukocyte Count; D008168:Lung / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology
chemical_list: D015850:Interleukin-6; D003907:Dexamethasone; D002738:Chloroquine; D015237:Dinoprost