title: Conditioned medium of dental pulp cells stimulated by Chinese propolis show neuroprotection and neurite extension in vitro.
abstract: The purpose of this study was to clarify the effect of Chinese propolis on the expression level of neurotrophic factors in dental pulp cells (DPCs). We also investigated that the effects of the conditioned medium (CM) of DPCs stimulated by the propolis against oxidative and endoplasmic reticulum (ER) stresses in human neuroblastoma SH-SY5Y cells, and on neurite extensions in rat adrenal pheochromocytoma PC12 cells. To investigate the effect of the propolis on the levels of neurotrophic factors in DPCs, we performed a qRT-PCR experiment. As results, NGF, but not BDNF and NT-3, in DPCs was significantly elevated by the propolis in a concentration-dependent manner. H2O2-induced cell death was significantly inhibited by the treatment with the CM of DPCs. In addition, the treatment with the propolis-stimulated CM of DPCs had a more protective effect than that with the CM of DPCs. We also examine the effect of the propolis-stimulated CM of DPCs against a tunicamycin-induced ER stress. The treatment with the propolis-stimulated CM as well as the CM of DPCs significantly inhibited tunicamycin-induced cell death. Moreover, the treatment with the propolis-stimulated CM of DPCs significantly induced neurite outgrowth from PC12 cells than that with the CM of DPCs. These results suggest that the CM of DPCs as well as DPCs will be an efficient source of new treatments for neurodegenerative diseases and that the propolis promote the advantage of the CM of DPCs via producing neurotrophic factors.
authors.affiliation: Lab Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ.,1-25-4 Daigaku-nishi, 1-1-1, Gifu 501-1196, Japan.
authors.forename: Daichi
authors.initials: D
authors.lastname: Kudo
authors.affiliation: Lab Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ.,1-25-4 Daigaku-nishi, 1-1-1, Gifu 501-1196, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Inden
authors.affiliation: Lab Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ.,1-25-4 Daigaku-nishi, 1-1-1, Gifu 501-1196, Japan.
authors.forename: Shin-Ichiro
authors.initials: S
authors.lastname: Sekine
authors.affiliation: Department of Oral and Maxillofacial Sciences, Gifu Univ. School of Medicine, Gifu, Japan.
authors.forename: Naritaka
authors.initials: N
authors.lastname: Tamaoki
authors.affiliation: Department of Oral and Maxillofacial Sciences, Gifu Univ. School of Medicine, Gifu, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Iida
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu Univ., Gifu, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Naito
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu Univ., Gifu, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Veterinary Medicine, Faculty Applied Biological Sciences, Gifu Univ., Gifu, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kamishina
authors.affiliation: Department of Oral and Maxillofacial Sciences, Gifu Univ. School of Medicine, Gifu, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Lab Medical Therapeutics and Molecular Therapeutics, Gifu Pharmaceutical Univ.,1-25-4 Daigaku-nishi, 1-1-1, Gifu 501-1196, Japan. Electronic address: hozumi@gifu-pu.ac.jp.
authors.forename: Isao
authors.initials: I
authors.lastname: Hozumi
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000378:metabolism / Q000494:pharmacology*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004285:Dogs; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D006801:Humans; D020932:Nerve Growth Factor / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism*; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D011429:Propolis / Q000494:pharmacology*; D051381:Rats
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D017077:Culture Media, Conditioned; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D011429:Propolis; D020932:Nerve Growth Factor
keywords: Conditioned medium; Dental pulp cells; Neurodegenerative disease; Propolis