title: Ectopic expression of myo-inositol 3-phosphate synthase induces a wide range of metabolic changes and confers salt tolerance in rice.
abstract: Salt stress is an important factor that limits crop production worldwide. The salt tolerance of plants is a complex biological process mediated by changes in gene expression and metabolite composition. The enzyme myo-inositol 3-phosphate synthase (MIPS; EC 5.5.1.4) catalyzes the first step of myo-inositol biosynthesis, and overexpression of the MIPS gene enhances salt stress tolerance in several plant species. In this study, we performed metabolite profiling of both MIPS-overexpressing and wild-type rice. The enhanced salt stress tolerance of MIPS-overexpressing plants was clear based on growth and the metabolites under salt stress. We found that constitutive overexpression of the rice MIPS gene resulted in a wide range of metabolic changes. This study demonstrates for the first time that overexpression of the MIPS gene increases various metabolites responsible for protecting plants from abiotic stress. Activation of both basal metabolism, such as glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle, and inositol metabolism is induced in MIPS-overexpressing plants. We discuss the relationship between the metabolic changes and the improved salt tolerance observed in transgenic rice.
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Kusuda
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Koga
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Miyako
authors.initials: M
authors.lastname: Kusano
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan; Faculty of Agriculture, Yamagata University, Tsuruoka-shi, Yamagata, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Oikawa
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan; Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
authors.forename: Masami Yokota
authors.initials: MY
authors.lastname: Hirai
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. Electronic address: ayosida@mail.ecc.u-tokyo.ac.jp.
authors.forename: Kaoru T
authors.initials: KT
authors.lastname: Yoshida
mesh_terms: D016166:Free Radical Scavengers / Q000378:metabolism; D019753:Intramolecular Lyases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D055442:Metabolome; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D055049:Salt Tolerance; D013312:Stress, Physiological
chemical_list: D016166:Free Radical Scavengers; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D019753:Intramolecular Lyases; C105497:D-myo-inositol-3-phosphate synthase
keywords: Abiotic stress; Abscisic acid; Metabolomics; Oryza sativa L.; ROS scavenger