title: Practical strategies for small-molecule probe development in chemical biology.
abstract: The effective identification, selection, and implementation of small molecules for the interrogation of biological systems require an intricate understanding of the chemical principles underlying their cellular activities. While much has been published regarding the use of screening techniques in forward chemical genetics platforms and on small-molecule target identification, less emphasis has been placed on detailed strategies for evaluating, selecting, and optimizing screening hits. This chapter provides practical tools for identifying and developing promising screening hit compounds into effective tools for biological discovery.
authors.affiliation: Department of Medicine - Cardiovascular Medicine, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN, USA, jonathan.e.hempel@vanderbilt.edu.
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: Hempel
authors.forename: Charles C
authors.initials: CC
authors.lastname: Hong
mesh_terms: D055808:Drug Discovery / Q000379:methods* / Q000592:standards; D053823:Hedgehog Proteins / Q000378:metabolism; D006801:Humans; D008024:Ligands; D015336:Molecular Probe Techniques; D015335:Molecular Probes; D011485:Protein Binding; D015398:Signal Transduction / Q000187:drug effects; D054852:Small Molecule Libraries
chemical_list: D053823:Hedgehog Proteins; D008024:Ligands; D015335:Molecular Probes; D054852:Small Molecule Libraries