title: Association between p53 codon 72 (Arg72Pro) polymorphism and primary open-angle glaucoma in Iranian patients.
abstract: Glaucomatous neuropathy is a type of cell death due to apoptosis. The p53 gene is one of the regulatory genes of apoptosis. Recently, the association between the p53 gene encoding for proline at codon 72 and primary open-angle glaucoma (POAG) has been studied in some ethnic groups. This study is the first association analysis of POAG and p53 codon 72 polymorphism in Iranian patients.
  
  
  
  A cohort of 65 unrelated patients with POAG (age range from 12-62 years, mean ± SD of 40.16 ± 17.51 years) and 65 unrelated control subjects (without glaucoma, age range of 14-63 years, mean ± SD of 35.64 ± 13.61 years) were selected. In Iranian POAG patients and normal healthy controls, the p53 codon 72 polymorphism in exon 4 was amplified using polymerase chain reaction. The amplified DNA fragments were digested with the BstUI restriction enzyme, and the digestion patterns were used to identify the alleles for the polymorphic site.
  
  
  
  Comparisons revealed significant differences in allele and genotype frequencies of Pro72Arg between POAG patients and control group. A higher risk of POAG was associated with allele Pro (OR = 2.1, 95% CI = 1.2-3.4) and genotype Pro/Pro (OR = 3.9, 95% CI = 0.13-12.7).
  
  
  
  The p53 Pro72 allele was more frequent in Iranian POAG patients than in the control group (P<0.05). The present findings show that the individuals with the Pro/Pro genotype may be more likely to develop POAG. However, additional studies are necessary to confirm this association.
authors.affiliation: Hematology, Oncology and Genetic Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Neamatzadeh
authors.affiliation: Dept. of Ophthalmology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd; Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Soleimanizad
authors.affiliation: Dept. of Microbial Biotechnology, Payam Noor University, Tehran, Iran.
authors.forename: Aref
authors.initials: A
authors.lastname: Atefi
authors.affiliation: Dept. of Medical Genetics, Shahid Sadoughi University of Medical Sciences and Health Services, 
  Yazd, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Zare-Shehneh
authors.affiliation: Dept. of Medical Genetics, Shahid Sadoughi University of Medical Sciences and Health Services, 
  Yazd, Iran.
authors.forename: Saba
authors.initials: S
authors.lastname: Gharibi
authors.affiliation: Dept. of Medical Genetics, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Shekari
authors.affiliation: Dept. of Hematology, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Amir Bahman
authors.initials: AB
authors.lastname: Rahimzadeh
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000235:genetics; D001483:Base Sequence; D002648:Child; D004247:DNA / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D005901:Glaucoma / Q000235:genetics*; D006801:Humans; D007492:Iran; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D055815:Young Adult
chemical_list: D016159:Tumor Suppressor Protein p53; D004247:DNA
keywords: Primary open-angle glaucoma; Glaucoma; p53; Iran