title: The role of miR-205 in the VEGF-mediated promotion of human ovarian cancer cell invasion.
abstract: OBJECTIVE
  Our objective was to investigate a miRNA pathway that acts downstream of VEGF-induced invasion of ovarian cancer cells.
  
  
  METHODS
  We used two paired high and low metastatic serous ovarian cancer cells to demonstrate the role of miR-205 in VEGF-induced invasion of ovarian cancer cells and to investigate the gene targets of miR-205.
  
  
  RESULTS
  Our previous comparative proteomics studies showed that VEGF decreased the expression of Ezrin and Lamin A/C, and this result was validated in the present study using qPCR and Western blotting. Then we found that VEGF enhanced the invasiveness of and inhibited apoptosis in ovarian cancer cells as assessed by transwell invasion assays and Annexin V-FITC immunostaining, respectively. VEGFR was also expressed in ovarian cancer cells, as assessed by immunocytochemical staining. Furthermore, using the dual-luciferase report assay system, we demonstrated that miR-205 targeted Ezrin and Lamin A/C. MiR-205 was up-regulated in ovarian cancer cells exposed to VEGF, as determined by miRNA microarray analysis and verified by qPCR. MiR-205 promoted the invasion and proliferation of ovarian cancer cells.
  
  
  CONCLUSIONS
  Our data reveal a new potential pathway in which VEGF promotes the invasion of ovarian cancer cells, partially via the down-regulation of Ezrin and Lamin A/C caused by increased expression of miR-205.
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Juanni
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Microarray Core Facility, University of Texas Southwestern Medical Center, 75070, USA.
authors.forename: Zexia
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Gong
authors.affiliation: Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China.
authors.forename: Puxiang
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Hailing
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Junpu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha 410008, China; Department of Pathology, School of Basic Medical Science, Central South University, Changsha 410013, China. Electronic address: xyw2007@csu.edu.cn.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000502:physiology; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D003598:Cytoskeletal Proteins / Q000096:biosynthesis / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D034904:Lamin Type A / Q000096:biosynthesis / Q000378:metabolism; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D034904:Lamin Type A; D035683:MicroRNAs; D042461:Vascular Endothelial Growth Factor A; D040281:Vascular Endothelial Growth Factor Receptor-1; D000099149:Ezrin; C494847:LMNA protein, human; C534425:MIR205, human; C467484:VEGFA protein, human; C501162:FLT1 protein, human
keywords: Ezrin; Invasion; Lamin A/C; MiR-205; Ovarian cancer; VEGF