title: In NMR tube transcription for rapid screening of RNA conformation.
abstract: A simple method for rapid structure screening for RNA by NMR is proposed. Target RNA is transcribed in a NMR tube and its spectra are measured without purification. The proposed method, in NMR tube transcription or INTT, was applied for three RNAs for which NMR spectra have been measured by using the conventionally purified samples. By the real-time measuring, increase of imino proton signals and decrease of NTP signals can be observed. It was confirmed that INTT spectra are in general similar to those obtained by the conventional method. INTT can be used for the first-step screening of RNA folding.
authors.affiliation: a Department of Life and Environmental Sciences, Faculty of Engineering , Chiba Institute of Technology , Narashino , Chiba , Japan.
authors.forename: Saya
authors.initials: S
authors.lastname: Okui
authors.forename: Gota
authors.initials: G
authors.lastname: Kawai
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009690:Nucleic Acid Conformation; D011522:Protons; D012313:RNA / Q000737:chemistry*; D013053:Spectrophotometry; D013696:Temperature; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D008024:Ligands; D011522:Protons; D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases
keywords: NMR; RNA; screening; structure